LOCUS       DAVGJL010000001        50236 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.001, whole genome shotgun sequence.
ACCESSION   DAVGJL010000001 DAVGJL010000000
VERSION     DAVGJL010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 50236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50236
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.001"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(125..583)
                     /locus_tag="VVE42_000001"
     CDS             complement(125..583)
                     /locus_tag="VVE42_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HEU3072279.1"
                     /translation="MKKQVFHDAAAGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWAAIGMLFNFGNRLFSRDWSMLRATLTHFFLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREAKKVDHINQLLEHRK"
     gene            complement(580..1020)
                     /locus_tag="VVE42_000002"
     CDS             complement(580..1020)
                     /locus_tag="VVE42_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU3072280.1"
                     /translation="MKVELQISETYEEEKLIVQAPQPTDKVQKVIEFAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVLKLLPSNFIQISQSE
                     IINIDSISHLKLTPNGLVEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            1376..3325
                     /gene="mutL"
                     /locus_tag="VVE42_000003"
     CDS             1376..3325
                     /gene="mutL"
                     /locus_tag="VVE42_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEU3072281.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKVQITDNGHGIAHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVENLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLVSA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMALVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVEQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            complement(3651..4118)
                     /locus_tag="VVE42_000004"
     CDS             complement(3651..4118)
                     /locus_tag="VVE42_000004"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEU3072282.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIEVAWVPGAFEIPLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(4119..5324)
                     /locus_tag="VVE42_000005"
     CDS             complement(4119..5324)
                     /locus_tag="VVE42_000005"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEU3072283.1"
                     /translation="MEYRKIQEALEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERNGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLVERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKA
                     MKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAQMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRM
                     GHILNMEEK"
     gene            complement(5344..5979)
                     /gene="ribE"
                     /locus_tag="VVE42_000006"
     CDS             complement(5344..5979)
                     /gene="ribE"
                     /locus_tag="VVE42_000006"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEU3072284.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSVNGRLGGHVVTGHI
                     DGTGKISSIKKDDNAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTLEQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNGF"
     gene            complement(5964..7064)
                     /gene="ribD"
                     /locus_tag="VVE42_000007"
     CDS             complement(5964..7064)
                     /gene="ribD"
                     /locus_tag="VVE42_000007"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEU3072285.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAVIVKDNHIIGQGYHE
                     FFGGPHAERNALKNCRESPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMSMDGK
                     IATKTNQSKWITEEEARKHVHQLRHHVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKIFGGSAKFPVGGEGISL
                     PNDAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     regulatory      complement(7198..7312)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            7469..8062
                     /gene="ruvA"
                     /locus_tag="VVE42_000008"
     CDS             7469..8062
                     /gene="ruvA"
                     /locus_tag="VVE42_000008"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEU3072286.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQEA
                     QIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDDLPAKVAVQASAENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     gene            8072..8635
                     /locus_tag="VVE42_000009"
     CDS             8072..8635
                     /locus_tag="VVE42_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEU3072287.1"
                     /translation="MTKRCSWVKMTNPLYIAYHDEEWGQPLHDDQALFELLCMETYQA
                     GLSWETVLNKRQAFREAFHSYQIHSVAEMTDTELEAMLENPAIIRNRAKIFATRANAQ
                     AFLQLQAEYGSFDAYLWSFVEGKTVVNDVPDYRQAPAKTPLSEKLAKDLKKRGFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWKGLK"
     gene            8632..9309
                     /locus_tag="VVE42_000010"
     CDS             8632..9309
                     /locus_tag="VVE42_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU3072288.1"
                     /translation="MMSNKNKGILIFAILYTVLFVFDGVKLLASLMPSAIANYLVYVV
                     LALYGSFLFKDRLIQQWKEIRKTKRKFFFGVLTGWLFLILMTVVFEFVSEMLKQFVGL
                     DGQGLNQSNIQSTFQEQPLLIAVFACVIGPLVEELFFRQVLLHYLQERLSGLLSIILV
                     GLVFALTHMHSLALSEWIGAVGYLGGGLAFSIIYVKEKENIYYPLLVHMLSNSLSLII
                     LAISIVK"
     gene            complement(9446..10477)
                     /locus_tag="VVE42_000011"
     CDS             complement(9446..10477)
                     /locus_tag="VVE42_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEU3072289.1"
                     /translation="MVSTIGIVSLSSGIIGEDFVKHEVDLGIQRLKDLRLNPIFLPHS
                     LKGLDFIKDHPEARAEDLIHAFSDDSIDMILCAIGGDDTYRLLPYLFENDQLQKVIKQ
                     KIFLGFSDTTMNHLMLHKLGIKTFYGQSFLADICELDKEMLAYSLHYFKELIETGRIS
                     EIRPSDIWYEERTDFSPTALGTPRVSHTNTGFDLLQGSAQFEGKILGGCLESLYDIFD
                     NSRYADSTELCQKYKLFPDLSDWEGKILLLETSEEKPKPEDFKKMLLTLKDTGIFAVI
                     NGLLVGKPMDETFHDDYKEALLDIIDSNIPIVYNLNVGHATPRAIVPFGVHAHVDAQE
                     QVILFDYNK"
     gene            complement(10732..11415)
                     /locus_tag="VVE42_000012"
     CDS             complement(10732..11415)
                     /locus_tag="VVE42_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEU3072290.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPQ
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGPDMDRSQRPPFWK
                     SVLVILASMFLWLLVFFATSFLPASLNPVLDPLPLAIIGAALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
     gene            complement(11427..12371)
                     /locus_tag="VVE42_000013"
     CDS             complement(11427..12371)
                     /locus_tag="VVE42_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEU3072291.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            12503..15334
                     /gene="uvrA"
                     /locus_tag="VVE42_000014"
     CDS             12503..15334
                     /gene="uvrA"
                     /locus_tag="VVE42_000014"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEU3072292.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAIKASSVEQIVDKVLELPERQRLQILAPVIRKKK
                     GQHKSVIEKIQKDGYVRVRVDGEVYDVTEVLELSKSRQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALRIAEGYVIIDTMDDSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPDASKTLREGALAPWNPISSNYYPNMLEQAMKVFGVAMDKP
                     FEDLSEEDKNLILYGSDGKEFHFHYENEFGGVRDIDIPFEGVINNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCQGYRLNDQALSVRVGGQQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVIGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDDGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            15327..16388
                     /locus_tag="VVE42_000015"
     CDS             15327..16388
                     /locus_tag="VVE42_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEU3072293.1"
                     /translation="MNKRVQAFLAKMQEKELDGIIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMGLTRIGFEDEISVSYY
                     HRMQAAFAGLNLLPQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASGLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKAGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITEKGCELLTLAPKELIVI"
     gene            16520..16918
                     /gene="spx"
                     /locus_tag="VVE42_000016"
     CDS             16520..16918
                     /gene="spx"
                     /locus_tag="VVE42_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEU3072294.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
     gene            16984..17553
                     /locus_tag="VVE42_000017"
     CDS             16984..17553
                     /locus_tag="VVE42_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEU3072295.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGPATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLTIQQKRPVSDELKTYIDQHGVE
                     ETQKALLEAEEKDKSIIEARKLAGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGLENLLKNEPSKYISDRLANGATEQ"
     gene            17640..17906
                     /locus_tag="VVE42_000018"
     CDS             17640..17906
                     /locus_tag="VVE42_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEU3072296.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            17910..18329
                     /gene="ruvX"
                     /locus_tag="VVE42_000019"
     CDS             17910..18329
                     /gene="ruvX"
                     /locus_tag="VVE42_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEU3072297.1"
                     /translation="MRIMGLDVGSKTVGVAISDPFGFTAQGLEIIQINEEQGQFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPVDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            18345..18650
                     /locus_tag="VVE42_000020"
     CDS             18345..18650
                     /locus_tag="VVE42_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEU3072298.1"
                     /translation="MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFMEE"
     gene            18908..20158
                     /locus_tag="VVE42_000021"
     CDS             18908..20158
                     /locus_tag="VVE42_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEU3072299.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRIQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLADQVKE
                     MEKTLLQTPDQLSFFELLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLVCQKKAESLAVDLYQA
                     GQDFSVLNGDFSSSLLNLSQLNIGLEGAYQQENAALALQTFLLFMGERKEAIDEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTRLVEFIKEKEQEGYRPQILFGSLKRKD
                     YQGMLGYLTENLPQVELKMTGFDYQGSLDETDVTGYHVIPSYREFISSFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            20155..20697
                     /locus_tag="VVE42_000022"
     CDS             20155..20697
                     /locus_tag="VVE42_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin/fatty acid-binding family protein"
                     /protein_id="HEU3072300.1"
                     /translation="MTPFFELVILGELLVRENFSKLVAERKFIMKLKRFTLSLASLAS
                     FSLLVACSQRAQQVQQPVAQQVQQPTQQNTNTANAGGNQNQAAPVQNQPVAQPTDIDG
                     TYTGQDDGDRITLVVTGTTGTWTELESDGDQKVKQVTFDSANQRMIIGDDVKIYTVNG
                     NQIVVDDMDRDPSDQIVLTK"
     gene            20843..22375
                     /gene="cls"
                     /locus_tag="VVE42_000023"
     CDS             20843..22375
                     /gene="cls"
                     /locus_tag="VVE42_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEU3072301.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIREIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            22633..24171
                     /locus_tag="VVE42_000024"
     CDS             22633..24171
                     /locus_tag="VVE42_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEU3072302.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWALHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     FFFAIFEWLALVAGGFFLFRSADTLTEQGDQAGHLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSLLFIAVVSLGLLVFNLG
                     HRRFVHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSLGLGFVVCIFLGLFQSKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRSARRSRRETPVSLWSRFFKGNLYLLVFGFVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDAIYAWDDTATLYRKSERLSPSAILS
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVVVWSEVETLLKENYQQVKTDYSEFKV
                     YKIK"
     gene            24291..26498
                     /gene="nrdD"
                     /locus_tag="VVE42_000025"
     CDS             24291..26498
                     /gene="nrdD"
                     /locus_tag="VVE42_000025"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEU3072303.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALHKAADKVMD
                     VTPLVEKRLNDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKWEDYAWK
                     KTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKDENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHHLGKEESVDQLFKNRRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTN"
     gene            26510..26650
                     /locus_tag="VVE42_000026"
     CDS             26510..26650
                     /locus_tag="VVE42_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3072304.1"
                     /translation="MGKYQLDDKGRAQVTRYHEKHSKGGAGKKERLLSFREQFLNKNK
                     KK"
     gene            26694..27200
                     /locus_tag="VVE42_000027"
     CDS             26694..27200
                     /locus_tag="VVE42_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU3072305.1"
                     /translation="MELRRPRLADKKAVLDMMTEFEKFQSPHDGGFWDTENFVYEDWL
                     ESNQEQEMGINLPEGWVPAIQLVAFSEKGQAVGFLNLRLRLSNFLLEEGGHIGYSIRP
                     SERGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNPASRAVILANGGIFEDARNGVER
                     YWIEVANE"
     gene            27193..27783
                     /gene="nrdG"
                     /locus_tag="VVE42_000028"
     CDS             27193..27783
                     /gene="nrdG"
                     /locus_tag="VVE42_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEU3072306.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVSGCMF
                     HCEGCYNVATWSFNAGIPYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELPDKDIWSWTGYTWEEMMLETPDKLELLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKESYEQVKRE"
     gene            27780..28397
                     /locus_tag="VVE42_000029"
     CDS             27780..28397
                     /locus_tag="VVE42_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HEU3072307.1"
                     /translation="MKKKDLVDQLVSEIETGKVRTLGIYGHGASGKSTFAQELYQALD
                     STTVNLLETDPYITSGRHLVVPKDAPNQKVTASLPVAHELESLQRDILALQAGMDVLT
                     IEEPWKASEVLSGAKPILIVEGMSVGFLPKELFEKTICFYTDEETELKRRLARDTTVR
                     NRDASFILASHQMRREQYLRYYKETESKADILVDQSENKFDVKRT"
     gene            28663..28971
                     /gene="rpsJ"
                     /locus_tag="VVE42_000030"
     CDS             28663..28971
                     /gene="rpsJ"
                     /locus_tag="VVE42_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEU3072308.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            29188..29814
                     /gene="rplC"
                     /locus_tag="VVE42_000031"
     CDS             29188..29814
                     /gene="rplC"
                     /locus_tag="VVE42_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEU3072309.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            29839..30462
                     /gene="rplD"
                     /locus_tag="VVE42_000032"
     CDS             29839..30462
                     /gene="rplD"
                     /locus_tag="VVE42_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEU3072310.1"
                     /translation="MANVTLFDQTGKEAGQVVLSDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQAAISKIEEVLA"
     gene            30462..30758
                     /locus_tag="VVE42_000033"
     CDS             30462..30758
                     /locus_tag="VVE42_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEU3072311.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            30776..31609
                     /gene="rplB"
                     /locus_tag="VVE42_000034"
     CDS             30776..31609
                     /gene="rplB"
                     /locus_tag="VVE42_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEU3072312.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            31713..31994
                     /gene="rpsS"
                     /locus_tag="VVE42_000035"
     CDS             31713..31994
                     /gene="rpsS"
                     /locus_tag="VVE42_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEU3072313.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            32006..32350
                     /gene="rplV"
                     /locus_tag="VVE42_000036"
     CDS             32006..32350
                     /gene="rplV"
                     /locus_tag="VVE42_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEU3072314.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIAILTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            32363..33016
                     /gene="rpsC"
                     /locus_tag="VVE42_000037"
     CDS             32363..33016
                     /gene="rpsC"
                     /locus_tag="VVE42_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEU3072315.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            33020..33433
                     /gene="rplP"
                     /locus_tag="VVE42_000038"
     CDS             33020..33433
                     /gene="rplP"
                     /locus_tag="VVE42_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEU3072316.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            33443..33649
                     /locus_tag="VVE42_000039"
     CDS             33443..33649
                     /locus_tag="VVE42_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEU3072317.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            33674..33934
                     /gene="rpsQ"
                     /locus_tag="VVE42_000040"
     CDS             33674..33934
                     /gene="rpsQ"
                     /locus_tag="VVE42_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEU3072318.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            33960..34328
                     /gene="rplN"
                     /locus_tag="VVE42_000041"
     CDS             33960..34328
                     /gene="rplN"
                     /locus_tag="VVE42_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEU3072319.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            34406..34711
                     /gene="rplX"
                     /locus_tag="VVE42_000042"
     CDS             34406..34711
                     /gene="rplX"
                     /locus_tag="VVE42_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEU3072320.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            34735..35277
                     /gene="rplE"
                     /locus_tag="VVE42_000043"
     CDS             34735..35277
                     /gene="rplE"
                     /locus_tag="VVE42_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEU3072321.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNVKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            35295..35564
                     /gene="rpsN"
                     /locus_tag="VVE42_000044"
     CDS             35295..35564
                     /gene="rpsN"
                     /locus_tag="VVE42_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEU3072322.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            35849..36247
                     /gene="rpsH"
                     /locus_tag="VVE42_000045"
     CDS             35849..36247
                     /gene="rpsH"
                     /locus_tag="VVE42_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEU3072323.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGPNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            36439..36975
                     /gene="rplF"
                     /locus_tag="VVE42_000046"
     CDS             36439..36975
                     /gene="rplF"
                     /locus_tag="VVE42_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEU3072324.1"
                     /translation="MSRIGNKVIVLPAGVELANNDNVVTVKGPKGELTREFSKDIEIR
                     VEGTEITLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            37059..37415
                     /gene="rplR"
                     /locus_tag="VVE42_000047"
     CDS             37059..37415
                     /gene="rplR"
                     /locus_tag="VVE42_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEU3072325.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            37433..37927
                     /gene="rpsE"
                     /locus_tag="VVE42_000048"
     CDS             37433..37927
                     /gene="rpsE"
                     /locus_tag="VVE42_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEU3072326.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            37899..38123
                     /gene="rpmD"
                     /locus_tag="VVE42_000049"
     CDS             37899..38123
                     /gene="rpmD"
                     /locus_tag="VVE42_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEU3072327.1"
                     /translation="MVFQFLIWHKKGDKMAQIKITLTKSPIGRIPSQRKTVVALGLGK
                     LNSSVIKEDNAAIRGMITAVSHLVTVEEVN"
     gene            38225..38665
                     /gene="rplO"
                     /locus_tag="VVE42_000050"
     CDS             38225..38665
                     /gene="rplO"
                     /locus_tag="VVE42_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEU3072328.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            38678..39988
                     /gene="secY"
                     /locus_tag="VVE42_000051"
     CDS             38678..39988
                     /gene="secY"
                     /locus_tag="VVE42_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEU3072329.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRTE"
     gene            40139..40777
                     /locus_tag="VVE42_000052"
     CDS             40139..40777
                     /locus_tag="VVE42_000052"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEU3072330.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAHALDKTLA
                     ELGIELEGIINIEVNPDSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQDINDVFSDIEKVLTNLK"
     gene            40894..41112
                     /gene="infA"
                     /locus_tag="VVE42_000053"
     CDS             40894..41112
                     /gene="infA"
                     /locus_tag="VVE42_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEU3072331.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
     gene            41137..41253
                     /gene="rpmJ"
                     /locus_tag="VVE42_000054"
     CDS             41137..41253
                     /gene="rpmJ"
                     /locus_tag="VVE42_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEU3072332.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            41271..41636
                     /gene="rpsM"
                     /locus_tag="VVE42_000055"
     CDS             41271..41636
                     /gene="rpsM"
                     /locus_tag="VVE42_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEU3072333.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            41654..42037
                     /gene="rpsK"
                     /locus_tag="VVE42_000056"
     CDS             41654..42037
                     /gene="rpsK"
                     /locus_tag="VVE42_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEU3072334.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            42080..43015
                     /locus_tag="VVE42_000057"
     CDS             42080..43015
                     /locus_tag="VVE42_000057"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEU3072335.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            43027..43413
                     /gene="rplQ"
                     /locus_tag="VVE42_000058"
     CDS             43027..43413
                     /gene="rplQ"
                     /locus_tag="VVE42_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEU3072336.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            43678..43944
                     /locus_tag="VVE42_000059"
     CDS             43678..43944
                     /locus_tag="VVE42_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEU3072337.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAVVSSDEKQDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            43954..45291
                     /locus_tag="VVE42_000060"
     CDS             43954..45291
                     /locus_tag="VVE42_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEU3072338.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPDINRAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALTETDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGAAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGREGDMIEFGGLLGTAPVMKVNGASSVDFISRGGQIPAPIHSFKN"
     gene            45535..46227
                     /locus_tag="VVE42_000061"
     CDS             45535..46227
                     /locus_tag="VVE42_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU3072339.1"
                     /translation="MVKVRLYLVRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGKIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEMIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSVTILEYEDGQFRVEVVGDRSYR
                     ELGREKMEEGSI"
     gene            complement(46330..47965)
                     /locus_tag="VVE42_000062"
                     /pseudo
     CDS             complement(46330..47965)
                     /locus_tag="VVE42_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            complement(47955..49046)
                     /locus_tag="VVE42_000063"
                     /pseudo
     CDS             complement(47955..49046)
                     /locus_tag="VVE42_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289361.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(49059..50180)
                     /locus_tag="VVE42_000064"
                     /pseudo
     CDS             complement(49059..50180)
                     /locus_tag="VVE42_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931989.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
BASE COUNT        15111 a         9491 c        11006 g        14628 t
ORIGIN      
        1 tagaaatcga tttgactgtc ctgatcgatt tatcctgttc ttatttcatt tcactatagt
       61 aaggtgcagc aggataaaga aaaaccgaga agtttctttc tcggatttgc ttattatgag
      121 gttcctattt tctatgctcc aagagttgat tgatgtgatc tactttttta gcctctcttt
      181 tatagagaat agcccagatg atgagataga caatcgcaaa ctcgataatg agctggaggt
      241 agaaaatcca gtggaaaggg aaccaaccag caagagttgc tagtgggaca aagccagcca
      301 gcataaggaa gaaatgagtc agagtggcac gaagcatgct ccagtcacgg ctgaataagc
      361 ggttgccaaa gttgaagagc ataccgatgg ctgcccagat aagtgtgcag tagagcaaga
      421 ccagggcacc gtgaacctga tgctgatcca tcacttggcc gatgagagag tagggattta
      481 gtggtgcgta ggtatttggt gcataaatga gtgaaaagag gatggagagg atgaggccga
      541 taagaacacc ggcagctgca tcgtgaaata cttgtttttt catagttcta atttctcctt
      601 gatggttttt agataacggc gtgaagagta ggtgaagctt tcgtttttca agaaaatttc
      661 taccagaccg tttggcgtga gcttgaggtg agagatggaa tcgatattga tgatttctga
      721 ttgggaaatt tggataaaat tgcttggcaa gagtttaaga acctgataga gtcgcaaatc
      781 aatgctgtag gtctgactcg cggtttctgc tagaaccttc cgattctcga tatagaagcg
      841 ctgaatcttg ccaatctcaa ctagatagac ctgatcatcg attttccctt tgattttttc
      901 tctttggtcc agattttctg cgaactcgat gactttctgg actttatctg tcggctgagg
      961 tgcttggaca atcagctttt cctcctcgta agtctcacta atctgtagtt ctactttcat
     1021 aaatttctct ccttgttttt catacaaaat taatctcact tcttgtacac ctttattaat
     1081 actcttcaaa aatctcttca aaccacgtca gcgtcgtctt gccgtaggat agttgttgac
     1141 tttgtcagtt ttatctacaa cctcaaaaca gtgttttgaa ctgtctgcgc ctagctttct
     1201 agtttgctct ttgattttta ttgagtatac cactatttta ctccctctag caagggactt
     1261 tgtctatgtg gagggattgg gctcctatgt ggtggagctt ttctgttctt tctgaaatat
     1321 ggtataatag cactaattaa tttctaggaa aatagataca gaaaggggct gaaagatgtc
     1381 tcatattatt gaattgccag agatgctggc aaaccaaatc gcagctggag aggtcattga
     1441 acgtcctgcc agtgtggtca aagagttggt agaaaatgcc attgacgcgg gctctagtca
     1501 gattatcatt gagattgagg aagctggtct caagaaggtt caaatcacgg ataacggtca
     1561 tggaattgcc cacgatgagg tggagttggc cctgcgtcgc catgcgacca gtaagataaa
     1621 aaatcaagca gatctctttc ggattcggac gcttggtttt cgtggtgaag ccttgccttc
     1681 tattgcgtct gttagtgtct tgactctgtt aacggcggtg gatggtgcta gtcatggaac
     1741 caagttagtc gcgcgtgggg gtgaagttga ggaagtcatc ccagcgacta gtcctgtggg
     1801 aaccaaggtt tgtgtggaga atctcttttt caacacgcct gcccgtctca agtatatgaa
     1861 gagccagcaa gcggagttgt ctcatatcat tgatattgtc aaccgtctgg gcttggccca
     1921 tcctgagatt tcttttagct tgattagtga tggcaaggaa atgacgcgga cagcagggac
     1981 tggtcaactg cgccaagcaa tcgcagggat ttacggtttg gtcagtgcca agaagatgat
     2041 tgaaattgag aactctgacc tagatttcga aatttcaggt tttgtgtcct tgcctgagtt
     2101 gactcgggct aaccgcaatt atatcagtct cttcatcaat ggccgttata tcaagaactt
     2161 cctgctcaat cgtgctattt tggatggttt cggaagcaag cttatggttg gacgttttcc
     2221 actggctgtc attcacatcc atatcgaccc ttatctagcg gatgtcaatg tacatccaac
     2281 caagcaagag gtgcggattt ccaaggaaaa agaactgatg gcgctggtct cagaagctat
     2341 tgcaaatagt ctcaaggaac aaaccttgat tccagatgcc ttggaaaatc ttgccaaatc
     2401 gaccgtgcgc aatcgtgaga aggtggagca aactattctc ccactcaaag aaaatacgct
     2461 ctactatgag aaaactgagc cgtcaagacc tagtcaaact gaagtagctg attatcaggt
     2521 agaattgact gatgaagggc aggatttgac cctgtttgcc aaggaaacct tggaccgatt
     2581 gaccaagcca gcaaaactgc attttgcaga gagaaagcct gctaactacg accagctaga
     2641 ccatccagag ttagatcttg ctagcatcga taaggcttat gacaaactgg agcgagaaga
     2701 agcatccagc ttcccagagt tggagttttt cggacaaatg cacgggactt atctctttgc
     2761 ccaagggcga gatggacttt acatcataga tcagcacgct gctcaggaac gggtcaagta
     2821 cgaggagtac cgtgaaagca ttggcaatgt tgaccagagc cagcagcaac tcctagtgcc
     2881 ctatatcttt gaatttcctg cggatgatgc cttgcgtctc aaggaaagaa tgcctctctt
     2941 agaggaagtg ggcgtctttc tagcagagta cggagaaaat caatttattc tacgtgaaca
     3001 tcctatttgg atggcagaag aagagattga atcaggcatc tatgagatgt gcgacatgct
     3061 ccttttgacc aaggaagttt ctatcaagaa ataccgagca gagctggcta tcatgatgtc
     3121 ttgcaagcga tctatcaagg ccaatcatcg tattgatgat cattcagcta gacaactcct
     3181 ctatcagctt tctcaatgtg acaatcccta taactgtcct cacggacgtc ctgttttggt
     3241 gcattttacc aagtcggata tggaaaagat gttccgacgt attcaggaaa atcacaccag
     3301 tctccgtgag ttggggaaat attaaaagta taaaaaagtc tgggaaaaat tttcaaaatc
     3361 agaaaaacgc ataaaatcag gtgttcaaaa accttgattt ttatgcgttt tatcatggaa
     3421 atagttactt cattttttcc taattctttt cgaaactctt tttaaacgac gtcagtttta
     3481 tcagtaatct caaaacagtg ttttgagcta attttgccag ttttgtctgt aacatcgaag
     3541 ttgtgtttta ccactctgcg actggtttcc tagtttgctc tatgattttc acagagtatt
     3601 aaattgcgat tttgccaagt ttctttattc gtctaaaagt agagtctgtt ctatgcgtct
     3661 aatgtacgaa ttaggttgac catttcaata gctccttgtg cacactcaga acccttattt
     3721 cctgctttgg taccagctcg ttctatggct tgttcaattg tatctgtcgt tagcacacca
     3781 aacataacag gaatttcgct atttaaactg atttgggcga ttcccttaga tacctcgcta
     3841 catacataat cataatgact tgtattccct ctaatgacag ctcccaagca gataattgca
     3901 tcatattttt tactttttgc catttttgat gcaatcagtg gtatttcaaa agctcctgga
     3961 acccaggcta cctctatatc tttctcgttt acattctctc ttttgagatt atctagtgct
     4021 ccagataata attttgaagt tataaattca ttaaatctcg ctacaacaat acctatttta
     4081 atattgtttg ctactaaatt accttcataa gtgttcattt atttttcctc catatttaaa
     4141 atgtgaccca ttcgattttt ctttgtttct aaataaaaac tatcgtaagg attggcttct
     4201 atttcgattg atattctact ggaaatggta attccatatt tttctaactg ttcaaccttg
     4261 tcaggattat ttgtcagtaa atgaagtgac tgaagtccca gatctttaag catttgtgct
     4321 ccaatatgat attctcttaa atcaccttca aagcctaatg caagattagc atcaagcgta
     4381 tccatgcctt gatcttgtaa atgataggct tttaatttat tgataagtcc aattcctcgt
     4441 ccctcctgtc gcaagtaaag taagacaccc gaaccattct caacaatcat tttcatagct
     4501 ttatcgaatt gctgtccaca atcgcaacgt aaagagccta aaacatctcc tgttaaacat
     4561 tcggagtgga cccgacataa tacattggct tcatcctcta tgtttcccat aataagagca
     4621 agatgatgtt ccccatttag tttatctata tagctaattg ctttgaaatt accgtatcta
     4681 gtaggcatat tgacagttga aactcgttct accagctgat catatacttt tctatattct
     4741 tgtaattctt tgatggtaat tagtggaatg ttgtgtttct tcgagaactg aattaaatca
     4801 tctgttctca tcattttgcc atcatgattc attatttcac aacataggcc acactctttt
     4861 agtccagcta attttaataa atcaacagtt gcttctgtgt gtccatttct ttctagaaca
     4921 ccaccttttt ttgcaattaa aggaaacatg tgtcctggcc tgcgaaaatc agagggtgtt
     4981 atatcttcag ctacacacat acgtgcggtc agtcctcttt cctcggcaga aatacctgtg
     5041 gtcgtttctt tataatcaat tgaaactgta aaagcagtct tatgattatc tgtattgttt
     5101 tcaaccatag gtgaaagcat taattgatta gctaaacttt cgctcatagg catacaaatt
     5161 aatcctttgg cataagtagc cataaaatta acattttctg ttgtagctgc ttgtgcagaa
     5221 caaattaagt ctccttcatt ttctctatcc ttgtcgtcta taacaagaac aagtcgtccc
     5281 ttctgcaatg cttctaatgc ttcttgtatt tttcgatatt ccattgactg attatccttt
     5341 ctgctaaaat ccattttgat ataatagttc cttcgatatt tctgattttg gagagttatc
     5401 catcagtttt tgcacatatt tacctaagat atcattttca agatttactg tactcccgac
     5461 ttgtttactc ttaagaatgg tttgttccaa ggtatgaggg ataacagata ctgaaaagtt
     5521 tactttggag actttagcga cagtcagact aatgccgtca attgtaatag atcctttttc
     5581 aactattaaa tctaaaattt ctttttgtgt gttgatttga taccatacag cattatcatc
     5641 tttttttatt gacgagattt ttcctgtacc atcaatgtgt cctgtaacga cgtgaccccc
     5701 aagtcgaccg ttgacagata aggctctttc tagattcacc tcacttccat gttttaatag
     5761 agtaagagct gttcgactcc atgtttcatt cattacatca actgtaaagg attgatgatt
     5821 gaaatgagta actgtaagac agataccatt tactgctata ctatcgccta aatggatatc
     5881 cgttaatatt tttgaggctt taattgatag tttacaatta cgagagtctt tctgtattct
     5941 ttcaactttt ccgatttctt caattattcc tgtgaacatg gataaatcac ttcactttct
     6001 atgagatagt catttcctat ttgagaaaaa gcataaggtt tcaatctaat agcgtcattt
     6061 ggcaaagaaa tgccttcacc tccgacagga aacttggcac tgcctccaaa aatttttggt
     6121 gcaatatata ttttcagctc atcaacaatt tgttgttcca aagcactcca attcattagg
     6181 ctgccccctt ctaggactag gctatcaatc tgcatgtttc ctagatgttg cattaaactc
     6241 gataagtcta tatgattgcc ttttttcttt atggaaagta tttcacagcc atgattttga
     6301 tatagcttca ttttattttt gtcttcagag gaagtggcaa tgtaagtttt aatatcattt
     6361 gctgttttta cgattttaga ggtaagagga gttcgtaaat gtgtatcgca tatgatacgg
     6421 ataggatttt tcccttcctc caatctacat gtcagcaaag gatcgtcttg aataacagta
     6481 ttgactccca ccataattgc actaacatgg tgtcgtaact gatgcacatg ctttcttgct
     6541 tcttcttcag taatccattt ggattgattt gttttagtgg ctatttttcc atccattgac
     6601 attgcatatt tcataaaaac atagggtaca tgctgggtaa tatactttct aaaacttttt
     6661 attaagttaa gacactcatt ttctaaaatt ccaacagtaa cttgaagatt attttcctca
     6721 agtatcttta ctccttttcc agatacaata ggattacagt ctaggcttcc aatgactact
     6781 cttgtaatac cactatcgat tatagcatct atacagggag gtgttttccc gaagtgacaa
     6841 cagggttcaa gtgttacata aagcgtcgct ccgacagggg attctctaca gtttttaaga
     6901 gcatttctct cagcatgtgg gccaccaaaa aactcatgat aaccttgtcc gatgatgtga
     6961 ttatctttta caataactgc gccgaccata ggattgggat tgacgtaacc agcccctttt
     7021 tgtgccagtt ttattgctaa tttcatatat tttgaatcgc tcatctcact acctccaaaa
     7081 aaatatacct tgaataaggg actactcaag gcatacaaaa gaaaacttat gcgattaaca
     7141 aaaatactct gaaatgacaa gtaatcattt cagagcacgc aaaaagcaca aatatacttt
     7201 tatcttcttt catccagact atactgtcgg ctttggaatt tcaccaaatc atgcctttcg
     7261 gctcgtgggc tataccaccg gtagggaatt gcaccctgcc ctgaagatag ttattcaatt
     7321 acagatgatt atagtactta attttgaata tgtcaacaga taaataccga ttgtttttga
     7381 tatactgtat ttgtgataat cgattctcgc tcctcggata aagaaaatat gatatactag
     7441 ataaacgaaa taagagagaa ggaacactat gtacgcatat ttaaaaggaa tcattaccaa
     7501 aattactgcc aaatacattg ttcttgaaac caatggtatt ggttatatcc tgcatgtggc
     7561 caatccttat gcctattcag gtcaggttaa tcaggaggct cagatttatg tgcatcaggt
     7621 tgtgcgtgag gacgcccatt tgctttatgg atttcgctca gaggatgaga aaaagctctt
     7681 tcttagtcta atttcggtct ctgggattgg tcctgtatca gctcttgcta ttatcgctgc
     7741 tgatgacaat gctggcttgg ttcaagccat tgaaaccaag aacatcacct acttgaccaa
     7801 gttccctaaa attggcaaga aaacagccca gcagatggtg ctggacttgg aaggcaaggt
     7861 agtagttgca ggagatgacc ttcctgccaa ggtcgcagtg caagcaagtg ctgaaaacca
     7921 agaattggaa gaagctatgg aagccatgtt ggctctgggc tacaaggcaa cagagctcaa
     7981 gaaaatcaag aaattctttg aaggaacgac agatacagct gagaactata tcaagtcggc
     8041 ccttaaaatg ttggtcaaat aggagcagag aatgacaaaa cgttgttcgt gggtcaagat
     8101 gaccaaccca ctctacatcg cctatcatga tgaggagtgg ggccagcccc tccatgatga
     8161 ccaagcgttg tttgagttgt tgtgtatgga aacctatcag gcaggcctgt cttgggaaac
     8221 ggtactcaac aaacgccaag ctttccgaga agcctttcat agctatcaaa ttcactcagt
     8281 cgcagagatg actgacactg aattggaagc catgctggag aatccagcta tcattcgaaa
     8341 tagagccaag atttttgcta cacgcgctaa cgcccaagcc tttctacagt tacaggcaga
     8401 gtacggctct tttgatgcct atctttggtc ttttgttgag gggaaaactg tcgttaacga
     8461 tgttcctgat tatcgccaag ccccagctaa aacaccctta tctgagaaat tagccaaaga
     8521 tctcaaaaaa cgaggcttca agttcacagg cccagtcgcc gtattgtctt ttctacaggc
     8581 tgcagggcta gttgatgacc acgagaatga ttgtgagtgg aaaggtctta aatgatgtct
     8641 aacaaaaata agggaattct gatttttgcg attctctata cagtcctctt tgtgtttgat
     8701 ggcgttaaat tgctggcttc tttaatgcca tctgccattg caaattatct tgtttatgta
     8761 gttttagctt tatatggctc cttcttgttc aaggatagat tgatccaaca atggaaggag
     8821 attagaaaga ctaaaagaaa attcttcttt ggagtcttaa caggatggct ctttctcatt
     8881 ctgatgactg ttgtctttga atttgtatca gagatgttga agcagtttgt gggactagat
     8941 ggacaaggtc taaatcagtc taatattcaa agtacctttc aagaacaacc actactgata
     9001 gctgtttttg cttgtgtcat tggacctctg gtagaagaat tatttttccg tcaggtctta
     9061 ttgcattact tgcaggaacg gttgtcaggt ttactaagca ttattctggt aggacttgtt
     9121 tttgctctga ctcatatgca cagtttggct ctatcagagt ggattggtgc agttggttac
     9181 ttaggtggag gccttgcctt ttctattatt tatgtgaaag aaaaagagaa tatctactat
     9241 cccctacttg ttcacatgtt aagcaacagc ctctccttaa tcattttagc tatcagtata
     9301 gtaaaatgaa atgagaacag gacaaatcga tttctaacaa tgttttagaa gtagaggtgt
     9361 actattctag tttcaatata ctgtaatatg tgatgaaaat gccagtaatg ataccgagaa
     9421 aaaagctgag aaacttttcc cagctttatt tgttatagtc aaagagaatg acttgttcct
     9481 gtgcatctac atgagcatgg accccaaagg gtacaattgc tcttggagtt gcgtggccga
     9541 cattcagatt atagacaatc gggatattgc tgtcaatgat atccaatagt gcctctttat
     9601 agtcgtcatg gaaagtttca tccataggtt ttccgaccaa gagtccattg atgaccgcga
     9661 atatgccagt gtcctttaaa gttagcaaca tctttttgaa gtcttctggc ttaggctttt
     9721 cttcgcttgt ttcgagcaag aggattttcc cttcccagtc tgacaagtca gggaaaagtt
     9781 tgtatttttg gcagagctcc gtgctatctg cgtatcgaga gttgtcaaag atatcgtaga
     9841 gggattcgag gcaaccaccg aggattttcc cctcgaactg ggcacttcct tgcaacaagt
     9901 caaaacctgt atttgtatga ctgacacgag gtgttcccag ggccgtggga ctaaaatcag
     9961 ttcgttcctc ataccaaatg tcactagggc ggatttctga aattcttccc gtctcaatca
    10021 attctttaaa gtagtgaagg ctataggcta gcatttcttt gtctaattca caaatgtctg
    10081 ctaaaaagga ttgaccataa aaagtcttga ttcctaattt atgcaacatg aggtggttca
    10141 tggttgtatc cgagaagcca agaaaaattt tttgtttgat aaccttttgg agttggtcat
    10201 tttcaaaaag ataaggtagc aagcgatagg tatcgtctcc accgatggca cataggatca
    10261 tgtcgatgct atcatcagaa aaggcatgaa tcaaatcctc tgcacgagct tcaggatggt
    10321 ccttgataaa gtctaatcct tttaacgaat ggggcaaaaa gatgggattg agtctcagat
    10381 ccttgagacg ttggataccc aagtccactt cgtgtttgac aaaatcctct ccgataatgc
    10441 cactagataa actaacaata ccaatagtag aaaccatatc tcatcctcct agaaatagat
    10501 tgagcctatt ttatcacaaa atataagaga aagctatgtg ggatttcaga agaatacttt
    10561 taatactctt cgaaaatctc ttcaaaccac gtcagtttta tttgcaacct caaagctgtg
    10621 ctttgagcaa cctgcagcta gcttcctagt ttgctctttg attttcattg aatataactc
    10681 aaaaaagtaa gtgaaaaagc aaccgcacct gcagttgctt ttatcgtttt cttattcttg
    10741 atagcgtgtt ggtcctgcac ttgcactacg gatattcaag cgtttcttga gatagaagcg
    10801 aagggctagg agggctgctc caataatagc tagtggcaat ggatccagta ctgggttaag
    10861 gctagctggt aggaagcttg ttgcaaagaa gacaagcaac caaaggaaca tagaagctag
    10921 gataactagt acagatttcc agaaaggtgg acgttgactg cgatccatat ctggtccata
    10981 gtattggtaa acaaagtagt acatcaagta gaaggcaaat ccaccaacca gtccaactaa
    11041 tagaagagta atcaatccat agccgaaagc ttggtctgcc gcaaagaagg ttgtgagggc
    11101 gctgacaagg gcaaagaggc tagtgatgaa aagagctgag tccataatca tcagttttgg
    11161 gtcatcattt tcttttggat gctctttttc gtactgctct ttgacagtga agctatgagc
    11221 ccaatgagtt ggtgcgccgt atagggaacg ggcagttgta cctttagatt gctcctcaag
    11281 gatttgggga atgacttcct caaaaatagt ctggatttca gcgtctgttt tcccatcttt
    11341 gatgaattgt tgggtagcaa tgtggacaaa ctcttggttt ttcttagtta atttttgtag
    11401 atcaatttga gacataggaa ctcctcttag aaccattttt tatggatgag atagagagtg
    11461 agcgagacac tcatagcaaa ggcgataaag acgattaacc agaaggcatt tggctctccg
    11521 tttaggggga tttcattatc cttaaagttc atcccgtagg cagaaaagac catggttggg
    11581 atggacatga cgatggtcac aagggccaag gttttcatga tgttgttctg gttgttagaa
    11641 atgatagagg caaaggtctc tgtcatagaa tgcaagacgt ttccataaat atctgccatc
    11701 tcgatggcct gttgggtttc aatcagggtg tcttcaagca ggtcttcgtc ctcaaggtat
    11761 ttcttgatat tgctggttga actggtcaat ttcttaatca cgcgctcatt tgttttgagg
    11821 gaggccttga aatagacgat agttttttcc aattccatga gctcaatcaa ttcttcatta
    11881 cgagttgatt gatgcagttg actttcgatt tgttcactct tgcggtcgat tgaacgaagg
    11941 gctgttaggt aaagctctgc attgcgataa agaatttgaa agataaaacg tgaacgcatg
    12001 aaggtataga aattacgcaa tcgacggttg ataaagacat caaggacagg tagtggttcc
    12061 aaacacgtag tgataatggt ttcctcagtg atgataatac caagtgggat ggttacgtag
    12121 taggtgcggt tatttctttc ctccgtgacc ggcacgtcta cgataatcag ggtatactcg
    12181 tcttcaatgg taatacgaga catttcttcc gcatcgagcg gtgctcgaag gtcggcaata
    12241 tcaatatcga aggtgttggc gatttcgagt gattcatttt gagtcggatt gacgagattg
    12301 atccaagtac ccggttcaag cgtatcgatc tctttaaatt cagttgttgt agagagaaaa
    12361 acttgtttca tatcccttat cctttctcat ttttcagatt ttttcacact gtactattat
    12421 actacaaaat cggccttttg ggtaatagaa tctcactttt tttggtataa tggtaagcaa
    12481 taatggacta gaaagaacaa agatgcaaga taaaattgtc attcatgggg cgcgtgccca
    12541 taatttaaaa aatattgatg tggagattcc gcgagacaag ttggttgtcg tgactggctt
    12601 gtcaggttca gggaagtcca gtctggcttt tgataccctc tatgcggagg gacaacgtcg
    12661 ctatgtagag agtttgtccg cctacgctcg tcagttcttg ggaaatatgg aaaaaccaga
    12721 tgtagatgcc attgatggtc tcagcccagc tatttctatc gaccagaaaa cgactagcaa
    12781 aaaccctcgc tcgacggtgg gaacaacgac tgaaatcaat gactatctgc gtctcctcta
    12841 cgcgcgtgtg gggacgcctt actgtatcaa cgggcatggg gctatcaagg cttcttctgt
    12901 ggagcaaatc gttgataagg ttttggagtt acctgaacgc cagcgcctgc agattttggc
    12961 ccctgtcatc cgtaagaaaa aaggacagca taagagcgtt atcgagaaga ttcagaaaga
    13021 tgggtatgtt cgtgtccgtg tggatgggga agtctatgat gtgaccgaag tgctagagtt
    13081 gtccaagagc agacaacaca atattgatgt cgtagttgac cgtattgtca tcaaggaggg
    13141 tattcgtagc cgtctctttg attccattga agctgccctt cgtattgcag aaggttatgt
    13201 cattatcgac accatggacg attcggagtt gctcttctct gagcattatg cctgtccagt
    13261 ttgtggtttt actgtcccag agttagagcc tcgtctcttc tctttcaatg ctccttttgg
    13321 ctcttgtagt gagtgtgacg gcttgggcat caagctggag gtggatactg atttggtagt
    13381 gccagatgcc agcaaaacct tacgtgaggg agcgctagca ccttggaatc ctatctcatc
    13441 caactactat ccaaacatgt tagagcaggc catgaaagtc tttggagtgg ctatggataa
    13501 gccttttgag gacctgtcag aagaagataa gaacttgatt ctctatgggt cagatggtaa
    13561 ggaattccat ttccactatg agaatgaatt tggtggcgtg cgcgatatcg acattccgtt
    13621 tgagggagtt atcaataata tcaaacgtcg ttaccatgaa accaatagcg attacactcg
    13681 cactcagatg cgtctctaca tgaatgagct gacctgcgga acctgtcaag gctatcgtct
    13741 caatgaccag gccttgtctg tccgtgtggg cggccagcaa gggccacata ttggagaaat
    13801 ctcagacctg tctatcgctg accacttgga cttggtgagc cagttaactt tgtctgaaaa
    13861 tgaagccatc attgctcgtc ccattctcaa ggaaatcaag gatcgtttga ccttccttaa
    13921 taatgtgggt cttaactatc taaccctgtc acgttcagca ggaacccttt caggtgggga
    13981 aagtcagcgc attcgtttgg caacccagat tggttccaac ctatcaggcg tcctctatat
    14041 cctagacgag ccgtcaatcg gtcttcacca gagggacaat gaccgcctga ttgccagtct
    14101 gaaaaagatg cgtgacttgg gcaatactct tatcgtggtg gaacacgacg aagataccat
    14161 gcgtgaagct gattatctga ttgacgttgg tcctggtgcc ggtgtttttg gtggtgagat
    14221 tgttgcagca ggtacaccta agcaagtggc tcgcaacagc aagtctatca caggtcagta
    14281 cttgtcaggc aaacgtgtca tcccagtacc agaagagcgc cgtgtcggaa atggtcgttt
    14341 tattgaagtg ataggagcgc gtgagaacaa cttgcaaaat gtcacggctc gctttccact
    14401 aggaaaattt atcgcagtga caggtgtgtc gggttcaggg aaatcgactc taatcaacag
    14461 cattctcaaa aaagccattg cccagaagct caaccgtaat tcagacaaac ctggtaagtt
    14521 taagactatt acagggattg agcatgtcga ccgtttgatt gatattgacc agagccctat
    14581 cggacggacg ccgaggtcta acccagctac ctatacagga gtttttgacg atatacgtga
    14641 cctttttgct cagacaaatg aagccaagat tcgaggctac aagaaggggc gcttcagttt
    14701 caacgtcaag ggaggtcgct gtgaagcttg ctcaggtgac gggattatca agattgagat
    14761 gcacttcttg ccagatgttt atgtggcttg tgaagtttgc cacggaaccc gctataacag
    14821 tgaaacccta gaagttcact acaaggaaaa gaatatttcg caggtcttgg atatgacggt
    14881 caacgatgcg gtagaatttt tccaacacat tccgaaaatt caacgcaaac tccagaccat
    14941 caaggatgta ggtctaggct atgtgacctt gggacagcca gctaccaccc tttctggggg
    15001 agaagcccaa cgtatgaagt tagctagcga actccacaaa cgctcgacag gaaaatcttt
    15061 ctacattctg gatgagccga cgacagggct tcatacagag gacattgctc gcctgctcaa
    15121 ggttttagct cgctttgtag acgacggcaa tacagtcctc gtcatcgagc acaatctgga
    15181 tgttatcaag acggcagacc atatcattga cttgggacct gagggcggtg tcggtggtgg
    15241 aaccatcatc gtaacaggaa ctccagaaga agtagctgcc aacgaagcca gctatacagg
    15301 acactatttg aaaggaaagt tacatcatga ataaacgcgt acaagcattt ctagctaaaa
    15361 tgcaagaaaa agaactagat ggtatcatca tcaacaatct taaaaacgtc tattatttga
    15421 ctggtttttg gggctcaaac ggaacagtct ttatcagccg tgaccgtcag gtcttagtga
    15481 cagactctcg ctatatcatc gcagctaagc aagaaaccag tggttttgag attgtggctg
    15541 atcgtgatga attggctgtc attgcaggaa ttgttaagga catgggcttg actcgtatcg
    15601 gttttgaaga tgagatttca gtgtcttatt accaccgtat gcaggcagct tttgcaggtt
    15661 tgaacttgct tccacaaact cagtttgtgg aaggtcttcg tatgattaag gatgaggcag
    15721 agattgcagc gattcgcaag gcttgttcta tctcagacca agctttccgc gatgcgcttg
    15781 actttatcaa accaggaaaa actgaaattg agattgccaa cttccttgat ttccgcatgc
    15841 gtgagttggg agcatctggc ttatcttttg atacgatcct agctagcggt atcaattctt
    15901 ctaaacccca tgcccatcca atgcacaaac cagtggagtt gggagaagcc attaccatgg
    15961 acttcggctg tctctatgac cactatgtca gtgatatgac ccggactatc tatctagggc
    16021 atgttagcga tgagcaggca gagatttaca atacggttct aaaagctaac caagccttga
    16081 ttgaccaagc taaggcaggc ttaggtttcc gtgactttga caaaatccct cgtgatatta
    16141 tcattgaggc aggttatggt gactacttta ctcacggcat tggccacggt attggtctgg
    16201 atatccatga ggaaccctac tttagtcaga cttctacaga aactattaag acaggtatgg
    16261 ccttgaccga tgaaccaggt atctatatcg aaggcaaata tggcgttcgt atcgaggatg
    16321 atatcctgat tacagagaaa ggttgtgaat tattgaccct agctccaaaa gagttgatag
    16381 tcatttagtt gtttgtcaag aaaaaagtac gaaaatgacg aaaaaagtca aaaaaaatta
    16441 aaaataggtc gcaagtcgga tgttttttat ggtataatag actaaactga tagtaacatg
    16501 tagcgaaagg ggtaggtaca tgattaaaat ttatacagtc tcaagttgta ctagctgtaa
    16561 aaaagcaaaa acctggctca atgcccacca gttaagttat aaagaacaaa accttggtaa
    16621 agaaggaatt acgagagaag aattactgga tattctaacc aaaacagata acggaatagc
    16681 cagcatcgtt tcgtctaaaa atcgctatgc caaagccctt ggagtggata ttgaagattt
    16741 gagtgtcaat gaagttctca atctgattat ggaaacaccg agaattttaa agagcccaat
    16801 ccttgtagat gaaaaacgcc tgcaagttgg ctacaaggaa gacgatattc gtgccttcct
    16861 accacgctct gtccgtaatg tagaaaatgc agaagcacgt ttgcgtgcag ctctataaac
    16921 atcaaggctg ggagcacttc ccagtcttat tctattttaa tttcaaaaag aaagaagaaa
    16981 gaaatgaaaa aaatagttct tgttagtcta gctttccttt ttgtcctggt tggttgcgga
    17041 cagaaaaaag aaactggacc agctacaaaa acagaaaaag atacgcttca gtcggcattg
    17101 ccagttattg aaaatgctga gaagaataca gttgtaacta agactttggt cttgcccaag
    17161 tcagatgatg gtagccagca gacacaaaca attacttaca aagacaagac ttttttgagt
    17221 ctaactatcc aacaaaaacg tccagtctct gatgagttga agacttatat tgaccaacat
    17281 ggagtggagg aaactcaaaa agctcttctt gaagcggagg agaaggataa gtctatcatt
    17341 gaagctcgta aattggcagg tttcaaactt gaaacaaaac tattgagcgc aacggaactt
    17401 caaacaacga ctagttttga ttttcaagtt ctggatgtca agaaggcttc ccagttggaa
    17461 catctgaaga atattggttt ggaaaatctt ttgaaaaatg aaccaagcaa atatatttca
    17521 gatagattgg caaatggcgc gacagaacaa tagaaaatcc aaataaatag actgcctgaa
    17581 ttgtagaagg taaggaatta tgctataatg gtactattct aaggaaagag ggtgttagaa
    17641 tgggatttac tgaagaaaca gtacgtttta aattggacga ttccaataaa aaagaaatta
    17701 gcgaaacttt gacagatgtt tatgcttcgt tgaacgataa gggttacaac ccaattaacc
    17761 aaatcgtagg ttacgtattg agtggagacc ctgcctacgt tcctcgttat aataatgcac
    17821 gaaatcaaat ccgtaagtat gagcgtgatg aaatcgttga ggaattggtt cgctactacc
    17881 tcaaaggaca aggagtcgat ctataaccta tgagaattat gggattggac gtcggttcaa
    17941 aaacggtagg ggtggcgatt agcgatccgt ttggttttac agctcaaggg cttgaaatca
    18001 tccagataaa tgaagaacaa ggccaatttg gttttgaccg cgttaaggaa ttggttgata
    18061 cttacaaggt ggaacgattt gtagtgggct tgcctaaaaa catgaacaat acaagtggac
    18121 cgcgcgtaga agctagtcaa gcctacggag caaagctaga agagtttttt ggtttaccag
    18181 tagactatca ggatgaacgc ttgacaacag tggctgctga gcgcatgttg attgaacaag
    18241 cagatatcag tcgcaataag cgcaagaaag tcattgataa gttagcagct cagctgattt
    18301 tacaaaatta tttagataga aaattttaat ataaaggaga ggctatgtca cacgatcata
    18361 accacgacca cgaagaacgt gaactaatca cactagtaga tgagcaagga aatgaaacct
    18421 tgtttgaaat ccttttgaca attgatggaa aagaagaatt tggtaaaaac tatgttcttc
    18481 tagtaccagt taacgcagaa gaagacgaag acggacaagt tgaaatccaa gcttactcat
    18541 tcattgaaaa tgaagatgga acagaaggcg aattgcaacc aatcccagaa gactcagaag
    18601 acgaatggaa catgattgaa gaagtcttca acagctttat ggaggagtga tacagtctgg
    18661 gagactgtat cagcccaact ccagaaatgg ggaagagttg gaacatccag tggatgtttt
    18721 tagcccagtt cctttaaata agagagaact ggtaggttcg ggggacgttt ttagcccacg
    18781 ttcacattca tagtagctag ttattagcta accaggtaag aggtcgggac gaaaatcctg
    18841 gccttgtttt tattagtaac tatactttaa tgcgataatt gattggactt ttgtcaagga
    18901 gatgtgtatg tttgaagtag aagaatggct tcatagtcgg attggtttga attttcgatc
    18961 aggtttgggc cgaatacagc aagcggtgga tttgttagga aatcctgagc agtcttaccc
    19021 tattatccac gtaacaggga ctaatgggaa aggatctacc attgctttta tgagggaatt
    19081 atttatgggg catggcaaaa aagttgcgac ctttacctcc cctcatatcg tatccatcaa
    19141 tgaccgaatt tgcattaatg ggcaacctat agcagacgca gactttatcc gtttggctga
    19201 tcaggtaaag gagatggaga aaacgcttct gcaaactcct gaccagttgt ccttttttga
    19261 attgctgacc ttggttgctt ttctttattt tagggagcag gaggtggatt tggttttatt
    19321 agaagtggga attggtggct tacttgacac gaccaatgtg gtaactggag agcttgctgt
    19381 catcacctcc attgggcttg accatcaaga aaccttgggt gatagtctag aagcaattgc
    19441 agagcagaaa gctggtattt tcaaggctgg taaaaaggca gtgattgcga aattgcctcc
    19501 agaagctaga cttgtctgtc agaaaaaagc cgaatcttta gctgttgacc tttatcaggc
    19561 aggtcaagat ttttcagtgc tgaatggtga tttttcaagc tctttactaa atctttcaca
    19621 gctgaacata ggcttagaag gagcctatca gcaggagaat gcggccttgg cgttgcaaac
    19681 ttttcttctt tttatgggag aaagaaagga agctattgat gaacaggctg taagaaaggc
    19741 cttggaacag acccattggg ctggtcgctt ggagcgtatt cgcccacaga tttacttgga
    19801 tggtgctcat aacctccctg ccttgactcg cttggttgag tttatcaaag aaaaagagca
    19861 ggaaggctat cgacctcaaa tcctctttgg atccttgaaa cgtaaggatt atcaagggat
    19921 gttgggttat ctgactgaaa atttacctca agtggaactc aaaatgaccg gctttgacta
    19981 tcaagggtct ttggacgaga cagatgtaac aggttaccat gtaattccct cttaccgaga
    20041 atttatcagc agtttcgaag aaagggcaga cgctcaagac ttgctgttcg ttacagggtc
    20101 tctctatttt atctcagaag tacggggcta cctgctggac cgtgagcaga taaattgacc
    20161 cccttttttg aacttgttat actaggggaa ttgctggtta gagaaaattt ctctaaattg
    20221 gtagcagaaa ggaaattcat catgaaatta aaaagattca cactttctct tgcttctcta
    20281 gcaagtttta gtctcttagt agcttgttca caaagagctc aacaggttca acagcctgtt
    20341 gctcagcagg tccaacagcc tactcaacag aataccaata ctgcaaatgc aggaggtaac
    20401 caaaatcaag cggctccagt acaaaaccaa cctgttgctc aaccgaccga tattgatggg
    20461 acttatactg gtcaggatga cggagaccgt atcactttag tggtaactgg aacgactggt
    20521 acatggactg agctcgaatc tgacggggat cagaaagtca aacaggttac atttgattca
    20581 gcaaatcaac gcatgattat tggcgatgat gtcaaaattt acactgtaaa cggtaatcaa
    20641 atcgtcgtag atgatatgga tagagaccca tcggaccaaa tcgttttaac taaataagac
    20701 tatgcaagta gaaagggctg gatttttcag cctttttact tttacctaga aaataatcat
    20761 aaatacttgc cttctaccga atacctgcgt tatactagta tcaattactt gtttttagca
    20821 ttcaaactat caaggagggg atatgaaata taggaaattt caattattga tgtccaagta
    20881 tggctttagt ctttcgatta tgctacttga actttgtctt gtttttggtc tctttcttta
    20941 tttaggacgc atggctccca ttttatggat tactgtcctc attctactga gtatcatcac
    21001 aatcatttcg atagtcaacc gtaatacgac tcctgagaat aaggtaacct ggttgttagt
    21061 agcctttgtg ccagtatttg gtcccttgct ctatctgatg tttggtgaaa ggcgattgtc
    21121 caaaaaagaa atcaaacaac tgaagaagct aggctctatg catttccaag aagcaaatag
    21181 ccagctacta aaagagaaat taaaagaaag tgacaaggca gcttatggag tcatcaagtc
    21241 cttattgagt atggatacca atgctgacat ctatgatcaa actgcctcta cattttttcc
    21301 taacggagaa gctatgtgga aaaagatggt agaagatctt aaaaaggctg agaaatttat
    21361 tttcttggaa tattacatta tagaagaagg tttgatgtgg aatcgcatac tagatatact
    21421 agagcaaaag gtagctcagg gtgtagaggt taagatgctc tatgatgata tcggctgtat
    21481 ggctacttta acaggagatt atgcacatcg acttcgtcag ctgggcatcg aggcccataa
    21541 attcaataaa gttattcctc gtttgacagt ggcttataat aacagagatc atagaaaaat
    21601 attgattgtt gatggtcaga tagcctatac tggtggggtc aatctggcag atgagtacat
    21661 taaccacgtc gagagatttg gttattggaa ggatagtgga attcgcttag acggactagc
    21721 agtaaaagct ctgacacgct tatttttgac cacttggtac attaatcgag gagaaattag
    21781 tgattttgat caatatcatt tagaaaatca ttctatcccg agtgacggtt taaccattcc
    21841 atacggaagt ggacccaagc caatttttcg agcgcaggta gggaaaaaag tttatcagag
    21901 tttaatcaat caagcaacag aatcggtcta tattacgaca ccttatttga ttatagatta
    21961 tgatttaaca gagacaatca aaaatgcagc tatgagaggg gtcgatgttc gaattatcac
    22021 cccttacata ccagataaga agttcattca gttagtcacg agaggagctt atcccgacct
    22081 tctttctgct ggtgttcgga tttatgagta tagtccaggt tttattcata gtaagcagat
    22141 gttggtagac gaagattttg cggtggtggg gacaatcaat ctcgactacc ggagcttggt
    22201 acaccattat gaaaatgcag tcttactcta taaaactcct tctataaggg aaatcgcccg
    22261 agattttcga aatatatttg cagattctca ggaagtctat cctcattcta tcaaaacgag
    22321 ctggtatcaa aagcttgtaa aagaaatcgc ccagctattc gcccctatct tataagaaat
    22381 ctagaactta ggatacaaac cagacctatt tttcttgtct tttacgaata gaaagaaaga
    22441 ggaggggaat tcatgctaac taaggaagaa gtgaatgata tgattgagtt caaacagaca
    22501 catttacacc gctttcggga aattgaaacc tttgtgaaac tatgcaaaaa atcactcaaa
    22561 cagccatcgc aagctgacaa tcctaggact ttttgatata ctaagacaga taaattgaac
    22621 aggagaaacg atatgagtgt gtatggtaga gtagaagaag ttcataagga gaatcgtgaa
    22681 cctctagaat accaaatcga acaagaatcg catcatcgtg aatcaagtcg tcttcctttg
    22741 gtgaaaattt tactatggag tacgcttgta acggggataa ctctaggagt accgctatta
    22801 ctcgacttaa tgagtgcaca agaagtgcag gatttttatg caggttgggc gcttcatcag
    22861 acagggaaga tttacagcga ctattatgga agtcaaggtt tgctttatta tttgctgact
    22921 tacgtgagtc agggcggatt tttctttgcc atctttgagt ggttagcctt ggtagcagga
    22981 ggatttttcc tttttagatc agcggacacc ttgacagagc aaggagacca agctggacat
    23041 ctggtgacta ttttttacat gctagttaca ggtcttgctt ttggtggagg ctatgcgact
    23101 cttttagcgc ttcctttctt attcgcagcc tttagtttag ttgcggctta cctaagcaat
    23161 ccaagccatg ataagggatt tgtacggatt gggctagctt tggcaggcgg atttttcttt
    23221 gctcccttat catcgctcct gtttattgct gtagtgagtt taggcttgtt ggtctttaac
    23281 cttgggcata gacgttttgt gcatgggttt tatcagtttc ttgcagtggc tttaggtttt
    23341 tcacttgtct tttatccaac tgcctactat agtgctgcaa caggaagttt tggggatgcg
    23401 attagtggta ttcgttatcc tattgacagt attcgctttg attttacttc taaaatttta
    23461 gagaatatgt ttttttatgg cttgttgtcc cttggtttgg gatttgtggt ttgtatcttt
    23521 ttaggtctct ttcaatccaa accctttaaa ctatacgtca tttcggttcc tgcaagtctt
    23581 gtggtaattt taggtttgat tttacttttc ttttcacaag agcctctgca cgcttcttat
    23641 ttgatggtcg tcttccctgt tttcctactt ttattggtaa ccaatattaa gagtcaacag
    23701 agggggcgta gtgctagaag aagccgaaga gaaacgccag ttagcctatg gagtcgtttc
    23761 ttcaaaggaa atctatatct gctagttttt gggtttgtct atcttttgtc tgttcctttt
    23821 ttgatgaagt ttgtccttta tccagtacct tatcaagaac gtaatcgtct tgctgatttg
    23881 gtaaaagagg agacaaatac ggaagatgct atctatgcat gggatgatac tgcgactctt
    23941 tatcgtaaga gtgagcgctt gtcgccatcg gcgattttgt ccccgttgca ctatacagca
    24001 actgaggaaa atcgtaataa gttacttaat gacttgaaag aaaaacaacc taaggtgatt
    24061 gtggtaaatg acaaggtggt agtctggtct gaagtggaaa cactcttaaa agaaaattac
    24121 caacaagtaa agactgatta ctcagagttt aaagtctata aaattaaata accaaatcaa
    24181 tatcttgtgt atttttaaaa attttaggat ttttaacaca agatattgat ttttcttttt
    24241 agagtggtat aatacttttt agaaagaaca ttttagaaaa gagcatgcgt atgattgcac
    24301 tagaagaaaa aattacaatt ttgccaactc tcttcgtcga gaaacgagat gggagacgtg
    24361 ttgtatttga tgtggacaag attgacaagg ctctccacaa ggcggctgac aaggttatgg
    24421 atgtgacacc cctggttgaa aaacgcctca atgatctgac tgagcgaatt attacagaaa
    24481 ttcatagtcg ctttccacag ggaattaaga tttacgaaat tcaaaatatc gtagaacatg
    24541 aactccttga agccaaagaa tatgcgctgg ctgaggagta tattacttat cggacacaga
    24601 gggattttga gcgctcaaaa gcgacggata tcaactttag tattcataaa cttctcaaca
    24661 aagaccagac agttgtcaat gaaaatgcta ataaagacag tgatgtcttt aacactcagc
    24721 gtgatttgac agcagggatt gttgggaaat caatcggact gcaaatgctt cctaagcacg
    24781 tagccaatgc ccaccaaaag ggggatatcc actatcacga tttggactac agtccctata
    24841 cccctatgac caactgctgt ttgattgatt ttaagggtat gttggaaaat ggttttaaga
    24901 ttggaaatgc agaggtagag agtcctaagt ctatccagac tgcgacagca cagatttctc
    24961 aaatcattgc caacgttgct tctagccagt acggtggctg ttcagctgac cgtatcgatg
    25021 aaattttggc gccttatgca gagaagaact atcaaaaaca tctcaaagat gcagaagagt
    25081 gggtattgcc tgaaaaatgg gaagattacg cttggaagaa aacgcaaaag gacatctacg
    25141 atgccatgca atctcttgag tatgaaatca atactctctt cacttcaaat ggacaaacac
    25201 cttttacttc gctaggtttt ggtctgggaa ccagtcgttt tgaacgagaa attcaaaaag
    25261 ctattttaaa cattcgcatc aagggtcttg gttcagaaca ccgtacggct atctttccta
    25321 aacttatttt cactctgaaa agaggcctta atttagagga aggaactccc aactacgaca
    25381 tcaagcagtt ggctcttgag tgtgcaacca aacggatgta tccagatgtt ttatcttacg
    25441 ataagattgt tgacttgaca ggatccttca aggtgcctat gggttgccgt tctttcctcc
    25501 aaggatggaa ggatgaaaat ggtgtagaag tcaattcagg tcgcatgaat ctgggtgttg
    25561 tgacggttaa tctgcctcgt attgctcttg agtctgaagg tgatatgaat aagttctggg
    25621 aaatcttcaa cgagcgaatg aatatcgcag aagatgctct tgtttaccgt gtcgaacgca
    25681 ctaaagaggc gacaccagcg aatgctccta tcctttatca gtacggtgct tttggccatc
    25741 atctaggtaa agaagaaagt gttgaccagc tctttaagaa tcgtcgtgcg accgtttcgc
    25801 tgggctatat cggcttgtat gaagtagcga cagttttctt tggtaacagc tgggaaagta
    25861 atccagatgc taaggaattc acgctagaca tcattcacga tatgaaacgc cgtgtagaag
    25921 agtggtcaga ccaatatggc taccatttct ctatctactc aacaccatcc gaaagtctga
    25981 cagaccgttt ctgccgacta gatatagaca agtttggctc tattcctgat atcacagaca
    26041 aggaatacta caccaactct ttccactacg atgttcgtaa aaatccaaca ccgtttgaaa
    26101 aattggactt tgagaaagtc tatccagaag caggtgcgtc aggtggtttc atccattatt
    26161 gtgagtatcc agtccttcag caaaatccaa aggccttgga agctgtctgg gattatgctt
    26221 atgaccgtgt aggctatcta ggcaccaata ctccgattga ccgttgctac aagtgtgact
    26281 ttgaagggga ttttgaacca actgagagag ggtttgcttg tccaaactgt ggcaatagcg
    26341 accctaaaac agtagatgtg gtgaagcgaa cttgtggcta cctaggtaat cctcaagcaa
    26401 gaccgatggt caacgggcgt cacaaggaaa tcgctgcgcg tgtcaaacat atgaatggtt
    26461 caacgattaa aatagctggg catcaagtaa caaattagaa agaaatgaaa tgggaaaata
    26521 tcaactagac gataaggggc gcgcacaagt gacccgttat cacgagaaac actctaaagg
    26581 tggagctggt aagaaagaac gcttgcttag cttcagagaa caatttttaa acaagaacaa
    26641 gaaaaaataa aagtgagagc cagctctcgc ttttctcata gtgggaggta aggatggaat
    26701 tacgcagacc aagattagcg gataagaaag ctgttttaga tatgatgaca gagtttgaaa
    26761 aatttcagtc gcctcacgac ggcggtttct gggatacaga gaactttgtg tatgaagact
    26821 ggttagaaag caatcaggaa caggaaatgg ggattaatct gcctgaagga tgggttcctg
    26881 caattcagtt agtggctttt tctgagaaag gtcaagcagt tggatttctt aatctccggt
    26941 tgcgcctcag taacttttta ctagaagaag gtgggcacat tggctactcc atccgtccat
    27001 ctgaaagagg caagggttat gcaaaagaga ctctccgtca gggcttgcaa gttgctaagg
    27061 aaaagaacat caagaaagct ctggtgacct gtagcgtgaa taatcctgct agcagagcag
    27121 tcattctagc aaatggtgga atatttgagg atgctcgcaa tggagtcgag cgttattgga
    27181 tagaggtagc gaatgaataa tccaaaacca caagaatgga aaagcgagga acttagtcaa
    27241 ggtcgtatca ttgactacaa ggcctttaac tttgttgatg gcgaaggcgt gcgtaactct
    27301 ctctatgtat caggctgtat gtttcactgc gagggatgtt ataatgttgc gacttggtct
    27361 tttaatgctg gcattcccta tacagcagaa ttagaagagc agatcatggc agaccttgcc
    27421 caaccctatg ttcaaggctt gactttgctg ggaggggagc cttttctcaa tactggcatt
    27481 ctcttgcctc tagttaaacg catccgaaag gaattgccag acaaggacat ttggtcctgg
    27541 acgggctaca cttgggaaga aatgatgttg gaaactccag ataaactgga actcttatca
    27601 ttgattgaca ttcttgtcga tggacgatac gatcgaacta agagaaatct catgctccag
    27661 tttcgaggtt cgtccaacca acgaattatc gatgtgcaaa aatcgctcaa aagtgggcaa
    27721 gtagtgattt gggacaagct caatgacgga aaagaaagct atgaacaggt gaagagagaa
    27781 tgaagaaaaa agacttagta gaccaactag tctcagagat cgagacgggg aaagtcagga
    27841 cactgggaat atacggtcat ggagcttcag gtaaatcaac ctttgcacag gaattgtacc
    27901 aagctttaga ttctactaca gtaaatttgc tagagacaga tccttatatc acctcaggac
    27961 gccatctggt agtacccaag gacgcgccga atcaaaaggt gacagccagt ctgccagtgg
    28021 cgcatgaact ggagagtttg cagagagata tccttgcctt gcaggcgggt atggatgtct
    28081 tgacaattga agaaccttgg aaggctagtg aggtcttgtc tggagccaaa ccaattttga
    28141 ttgtcgaagg gatgtctgtt ggctttctac ccaaggaact ctttgaaaaa accatctgtt
    28201 tctacacgga tgaggagacc gaattaaagc gacgccttgc tagagatacg actgtgagaa
    28261 atcgcgatgc atcctttata ttggctagcc atcagatgag acgggagcag tatctgcgat
    28321 actataaaga aactgagtct aaggcggata tcttagtgga ccaatcagaa aataaatttg
    28381 atgtcaagag gacttaactt atgtatagaa gaaatataga agaaaatccc taattttaga
    28441 aagaaaaagt aggaaaacag tttcatccca aaaaaacgaa aaaacctaac aaatcccttg
    28501 caatcgcagg ggatttgtgt tattctatta tggtgctgta aattacagcc ttagttttga
    28561 tgcaagaggt tgcgacacgc tcggttgcat tgccacgcaa caccgcgtcg gttttcttgt
    28621 ggagctagcc tattatctta aatagacgaa aaggagaaaa agatggcaaa caaaaaaatc
    28681 cgtatccgtt tgaaagctta cgaacaccgt acgcttgaca cagcggctgc aaaaatcgta
    28741 gaatcagcta ctcgtacagg tgcacaagtt gcgggtccaa tcccacttcc aactgaacgt
    28801 agcctctaca caatcattcg tgcgactcac aaatacaaag actctcgcga acaatttgaa
    28861 atgcgtacac acaaacgttt gatcgatatc gttaacccaa ctcaaaaaac agttgatgcc
    28921 ttgatgaaat tggatcttcc aagtggtgta aacgtagaaa tcaaacttta atctaaaata
    28981 taaaagagca gaggctggtg tttcaatcta attgaacacg ggctaaactc ggtgtgaaaa
    29041 agataaactt cctagtgtct gctagacact gcgtcagttt cctattttca cgttgagttt
    29101 gacgcccttt gtatcttaga cttgagcata aaaaacgctc gttaaaaact ttttgaataa
    29161 aaaatataga aaaggaacta ttttctcatg acaaaaggaa tcttagggaa aaaagtggga
    29221 atgactcaaa tcttcactga agctggcgaa ttgatccctg taacagttat tgaagcaact
    29281 ccaaacgttg ttcttcaagt taaaactgtt gaaacagacg gatacaacgc tatccaagtt
    29341 ggtttcgatg acaaacgcga agtattgagc aacaaacctg ctaaaggaca tgtagcgaaa
    29401 gctaacacgg ctcctaagcg cttcattcgt gaattcaaaa acgttgaagg cttggaagtt
    29461 ggtgctgaaa ttacagttga aacattcgca gctggagacg ttgttgacgt aactggtact
    29521 tctaaaggta aaggtttcca aggtgttatc aaacgccacg gacaatcacg tggaccaatg
    29581 gctcacggtt ctcgttacca ccgtcgtcca ggttctatgg gacctgttgc acctaaccgc
    29641 gtattcaaag gtaaaaacct tgcagggcgt atgggtggcg accgcgtaac aattcaaaac
    29701 cttgaagttg tacaagttgt tccagaaaag aacgttatcc ttatcaaagg taacgtacca
    29761 ggtgctaaga aatctcttat cactatcaaa tcagcagtta aagctggtaa ataataaaga
    29821 aaggggaaat cagtcacaat ggcaaacgta acattatttg accaaactgg taaagaagct
    29881 ggccaagttg ttcttagcga tgcagtattt ggtatcgaac caaatgaatc agttgtgttt
    29941 gatgtaatca tcagccaacg cgcaagcctt cgtcaaggaa cacacgctgt taaaaaccgc
    30001 tctgcagtat caggtggtgg acgcaaacca tggcgtcaaa aaggaactgg acgtgctcgt
    30061 caaggttcta tccgctcacc acaatggcgt ggtggtggtg ttgtcttcgg accaactcca
    30121 cgttcatacg gctacaaact tccacaaaaa gttcgtcgcc tagctcttaa atcagtttac
    30181 tctgaaaaag ttgctgaaaa caaattcgta gctgtagacg ctctttcatt tacagctcca
    30241 aaaactgctg aatttgcaaa agttcttgca gcattgagca tcgattctaa agttcttgtt
    30301 atccttgaag aaggaaatga attcgcagct ctttcagctc gtaaccttcc aaacgtgaaa
    30361 gttgcaactg ctacaactgc aagtgttctt gacatcgcaa atagcgacaa acttcttgtc
    30421 acacaagcag ctatctctaa aatcgaggag gttcttgcat aatgaatttg tatgatgtta
    30481 tcaaaaaacc tgtcatcact gaaagctcaa tggctcaact tgaagcaggg aaatatgtat
    30541 ttgaagttga cactcgtgca cacaaacttt tgatcaagca agctgttgaa gctgctttcg
    30601 aaggtgttaa agttgccaat gttaacacaa tcaacgtaaa accaaaagct aaacgtgttg
    30661 gacgttacac tggttttact aacaaaacta aaaaagctat catcacactt acagctgatt
    30721 ctaaagcaat cgagttgttt gctgctgaag ctgaataatc taaggaggaa atatcgtggg
    30781 aattcgtgtt tataaaccaa caacaaacgg tcgccgtaat atgacttctt tggatttcgc
    30841 tgaaatcaca acaagcactc ctgaaaaatc attgcttgtt gcattgaaga gcaaggctgg
    30901 tcgtaacaac aacggtcgta tcacagttcg tcaccaaggt ggtggacaca aacgtttcta
    30961 ccgtttggtt gacttcaaac gtaataaaga caacgttgaa gcagttgtta aaacaatcga
    31021 gtacgatcca aaccgttctg caaacatcgc tcttgtacac tacactgacg gtgtgaaagc
    31081 atacatcatc gctccaaaag gtcttgaagt aggtcaacgt atcgtttcag gtccagaagc
    31141 agatatcaaa gtcggaaacg ctcttccact tgctaacatc ccagttggta ctttgattca
    31201 caacatcgag ttgaaaccag gtcgtggtgg tgaattggta cgtgctgctg gtgcatctgc
    31261 tcaagtattg ggttctgaag gtaaatatgt tcttgttcgt cttcaatcag gtgaagttcg
    31321 tatgattctt ggaacttgcc gtgctacagt tggtgttgtc ggaaacgaac aacatggact
    31381 tgtaaacctt ggtaaagcag gacgtagccg ttggaaaggt atccgcccaa cagttcgtgg
    31441 ttctgtaatg aaccctaacg atcacccaca cggtggtggt gaaggtaaag caccagttgg
    31501 tcgtaaagca ccatctactc catggggcaa acctgctctt ggtcttaaaa ctcgtaacaa
    31561 gaaagcgaaa tctgacaaac ttatcgttcg tcgtcgcaac gagaaataat attaaactag
    31621 tcgcttaagc aactagtaaa tccgccagct cggtagcgct ccataggagt gcaagccgct
    31681 gtggtacaac atttaaagga gaaaatataa aaatgggacg cagtcttaaa aaaggacctt
    31741 tcgtcgatga gcatttgatg aaaaaagttg aagctcaagc taacgacgaa aagaaaaaag
    31801 ttattaaaac ttggtcacgt cgttcaacga tcttcccaag tttcattggt tacactattg
    31861 cagtttatga cggacgtaaa cacgtacctg tttacatcca agaagacatg gtaggccaca
    31921 aacttggtga atttgcacca actcgtactt acaaaggtca cgctgcagac gacaagaaaa
    31981 cacgtagaaa ataaggagaa cataaatggc agaaattact tcagctaaag caatggctcg
    32041 tacagtacgt gtttcacctc gtaaatcacg tcttgttctt gataacatcc gtggtaaaag
    32101 cgtagccgat gcaatcgcaa tcttgacatt cactccaaac aaagctgctg aaatcatctt
    32161 gaaagttttg aactcagctg tagctaacgc tgaaaacaac tttggtttgg ataaagctaa
    32221 cttggtagta tctgaagcat tcgcaaacga aggaccaact atgaaacgtt tccgtccacg
    32281 tgcgaaaggt tcagcttcac caatcaacaa acgtacagct cacatcactg tagctgttgc
    32341 agaaaaataa ggaggtaaaa tcgtgggtca aaaagtacat ccaattggta tgcgtgtcgg
    32401 catcatccgt gattgggatg ccaaatggta tgctgaaaaa gaatacgcgg attaccttca
    32461 tgaagatctt gcaatccgta aattcgttca aaaagaactt gctgacgcag cagtttcaac
    32521 tattgaaatc gaacgcgcag taaacaaagt taacgtttca cttcacactg ctaaaccagg
    32581 tatggttatc ggtaaaggtg gtgctaacgt tgatgcactc cgtgcaaaac ttaacaaatt
    32641 gactggaaaa caagtacaca tcaacatcat cgaaatcaaa caacctgatt tggatgctca
    32701 ccttgtaggt gaaggaattg ctcgtcaatt ggagcaacgt gttgctttcc gtcgtgcaca
    32761 aaaacaagca atccaacgtg caatgcgtgc tggagctaaa ggaatcaaaa ctcaagtatc
    32821 aggtcgtttg aacggtgcag atatcgcccg tgctgaagga tactctgaag gaactgttcc
    32881 gcttcacaca cttcgtgcag atatcgatta cgcttgggaa gaagcagata ctacatacgg
    32941 taaacttggt gttaaagtat ggatctaccg tggtgaagtt cttccagctc gtaaaaacac
    33001 taaaggaggt aaataaccaa tgttagtacc taaacgtgtt aaacaccgtc gtgaattccg
    33061 tggaaaaatg cgcggtgaag caaaaggtgg aaaagaagta gcattcggtg aatacggtct
    33121 tcaagctaca actagccact ggatcactaa ccgccaaatc gaagctgctc gtatcgccat
    33181 gactcgttac atgaaacgtg gtggtaaagt ttggattaaa atcttcccac acaaatcata
    33241 cactgctaaa gctatcggtg tgcgtatggg atctggtaaa ggggcacctg aaggttgggt
    33301 agcaccagtt aaacgtggta aagtgatgtt cgaaatcgct ggtgtatctg aagagattgc
    33361 acgtgaagcg cttcgacttg ctagccacaa attgccagtt aaatgtaaat tcgtaaaacg
    33421 tgaagcagaa taaggagaag gcatgaaact taatgaagta aaagaatttg ttaaagaact
    33481 tcgtggtctt tctcaagaag aactcgcgaa gcgcgaaaac gaattgaaaa aagaattgtt
    33541 tgaacttcgt ttccaagctg ctactggtca attggaacaa acagctcgct tgaaagaagt
    33601 taaaaaacaa atcgctcgta tcaaaacagt tcaatctgaa gcgaaataat agactaggga
    33661 aggagaaatt tcaatggaac gcaataatcg taaagttctt gttggacgtg ttgtatctga
    33721 caaaatggac aagacaatca cagttgtagt tgaaacaaaa cgtaaccacc cagtctatgg
    33781 taaacgtatt aactactcta aaaaatacaa agctcatgat gaaaacaatg ttgccaaaga
    33841 aggcgatatc gtacgtatca tggaaactcg cccgctttca gctacaaaac gtttccgtct
    33901 tgtagaagtt gttgaagaag cggtcatcat ctaatcaaac ctgaaaggag aaaactgaaa
    33961 tgattcaaac agaaactcgt ttgaaagtcg cagacaacag cggtgctcgc gaaatcttga
    34021 ctatcaaagt tcttggtggt tcaggacgta aatttgcaaa catcggtgat gttatcgtgg
    34081 catctgtaaa acaagctact cctggtggtg cggttaaaaa aggtgacgtt gttaaagcag
    34141 ttatcgttcg tactaaatca ggtgctcgtc gtgctgatgg ttcatacatc aaatttgacg
    34201 aaaacgcagc agttatcatc cgtgaagaca aaactcctcg cggaacacgt atctttggcc
    34261 cagttgcacg tgaattgcgt gaaggtggct tcatgaagat cgtgtcactt gctccagaag
    34321 tactttaatt tttaggaaca aactagtccc ctagcttcaa gctagggtgc ccttatgggc
    34381 gtaagaaaaa tcaaggagaa acctaatgtt tgtaaaaaaa ggcgacaaag ttcgcgtaat
    34441 cgctggtaaa gataagggaa cagaagctgt tgtccttact gcccttccaa aagtaaacaa
    34501 agttatcgtt gaaggtgtta acattgttaa gaaacaccaa cgtccaacta acgagcttcc
    34561 tcaaggtggt atcatcgaga aagaagcagc tatccacgta tcaaacgttc aagttttgga
    34621 caaaaatggt gtagctggtc gtgttggtta caaatttgta gacggtaaaa aagttcgcta
    34681 caacaaaaaa tcaggcgaag tgcttgatta atcacgaagg aaaggagaag tataatggca
    34741 aatcgtttaa aagaaaaata tcttaatgaa gtagttcctg ctttgacaga acaattcaac
    34801 tactcatcag tgatggctgt gcctaaagta gataagattg ttttgaacat gggtgttggt
    34861 gaagctgtat caaacgttaa aagccttgaa aaagctgctg aagaattggc acttatctca
    34921 ggtcaaaaac cacttatcac taaagctaaa aaatcaatcg ccggcttccg tcttcgtgaa
    34981 ggtgttgcga tcggtgcaaa agttaccctt cgtggtgaac gtatgtacga attcttggat
    35041 aaattggtat cagtttcact tccacgtgta cgtgacttcc acggtgttcc aacaaaatca
    35101 tttgatggac gcgggaacta cacacttggt gtgaaagaac aattaatctt cccagaaatc
    35161 aacttcgatg acgttgacaa aactcgtggt cttgacatcg ttatcgtaac aactgctaac
    35221 actgacgaag agtcacgtgc attgcttaca ggccttggaa tgccttttgc aaaataatat
    35281 aggaggtaaa tctaatggct aaaaaatcaa tggtagctag agaggctaaa cgccaaaaaa
    35341 ttgttgaccg ttatgctgaa aaacgtgctg cattaaaggc ggcaggggac tacgaaggtt
    35401 tatctaaatt acctcgcaac gcctcaccga ctcgtttaca taatcgttgt agagttacgg
    35461 ggcgcccaca ttcagtttac cgcaaatttg gtctgagtcg tatcgctttt cgcgaacttg
    35521 cgcataaagg tcaaattcct ggtgtaacaa aagcatcttg gtaatttaag atatcaagag
    35581 cgtcaaaact ccaagtaaaa ataggaaact tgacgaagaa actaaagttt ctaggaaagt
    35641 ttatcttttt cacacagagt ttagcccggg ttcaattggg cttgccaatt tgaacacgag
    35701 ctacagcttt ggcaaaaaag accaatttgc tttggagcat tgcttctgca ttaaattgtc
    35761 tatttttgct cgtgctgtta cgctctttgt atcatgtatt aactagcaag tgcaacttgc
    35821 aaactactag taagaggaga aaaacaaaat ggttatgact gacccaatcg cagacttcct
    35881 aactcgtatt cgtaatgcta accaagctaa acacgaagta cttgaagtac ctgcatcaaa
    35941 catcaaaaaa gggattgctg aaatccttaa acgcgaaggt tttgtaaaaa acgttgaaat
    36001 cattgaagat gacaaacaag gcgtcatccg tgtatttctt aaatacggac caaatggtga
    36061 gaaagttatc actaacttga aacgtgtttc taaaccagga cttcgtgtct acaaaaaacg
    36121 tgaagacctt ccaaaagttc ttaacggact tggaattgcc atcctttcaa cttctgaagg
    36181 tttgcttact gataaagaag cacgccaaaa gaatgttggt ggtgaggtta tcgcttacgt
    36241 ttggtaaaat caagatacaa agctcgtaaa gaacaaagca aaattaggaa gttggagaag
    36301 tttgtttaca aacaagccaa cttatctatt ttgcacagtt cttagagcgt gttcagttca
    36361 gctcttgaac taaataagta tctgaacccc gtgaaaactg gccgttctgg cctgacaatt
    36421 taacaggaga aaataaacat gtcacgtatt ggtaataaag ttatcgtgtt gcctgctggt
    36481 gttgaactcg ctaacaatga caacgttgta actgtaaaag gacctaaagg agaacttact
    36541 cgtgagttct caaaagatat tgaaatccgt gtggaaggta ctgaaataac tcttcaccgt
    36601 ccaaacgatt caaaagaaat gaaaactatc cacggaacta ctcgtgccct tttgaacaac
    36661 atggttgttg gtgtatcaga aggattcaag aaagaacttg aaatgcgtgg ggttggttac
    36721 cgtgcacagc ttcaaggatc taaacttgtt ttggctgttg gtaaatctca tccagacgaa
    36781 gttgaagctc cagaaggaat tacttttgaa cttccaaacc caacaacaat cgttgttagc
    36841 ggaatttcaa aagaagtagt tggtcaaaca gctgcttacg tacgtagcct tcgttcacca
    36901 gaaccatata aaggtaaagg tatccgttac gttggtgaat tcgttcgccg taaagaaggt
    36961 aaaacaggta aataatgttg agtggttgat catcaaccac caacctattt tccaactttg
    37021 tgcatagcac acgatttaaa actaaagagg tgaaaactgt gatttcaaaa ccagataaaa
    37081 acaaactccg ccaaaaacgc caccgtcgcg ttcgcggaaa actctctgga actgctgatc
    37141 gcccacgttt gaacgtattc cgttctaata caggcatcta cgctcaagtg attgatgacg
    37201 tagcgggtgt aacgctcgca agtgcttcaa ctcttgataa agaagtttca aaaggaacta
    37261 aaactgaaca agccgttgct gtcggtaaac tcgttgcaga acgtgcaaac gctaaaggta
    37321 tttcagaagt ggtgttcgac cgcggtggat atctatatca cggacgtgtg aaagctttgg
    37381 ctgatgcagc tcgtgaaaac ggattgaaat tctaatagga ggacactaga aaatggcatt
    37441 taaagacaat gcagttgaat tagaagaacg cgtagttgct gtcaaccgtg ttacaaaagt
    37501 tgttaaaggt ggacgtcgtc ttcgtttcgc agctcttgtt gttgttggtg accacaatgg
    37561 tcgcgtagga tttggtactg gtaaagctca agaagttcca gaagcaatcc gtaaagcagt
    37621 agatgatgct aagaaaaact tgatcgaagt tcctatggtt ggaacaacaa tcccacacga
    37681 agttctttca gaattcggtg gagctaaagt attgttgaaa cctgctgtag aaggttctgg
    37741 agttgccgct ggtggtgcag ttcgtgccgt tgtggaattg gcaggtgtgg cagatattac
    37801 atctaaatca cttggttcta acactccaat caacattgtt cgtgcaactg ttgaaggttt
    37861 gaaacaattg aaacgcgctg aagaaattgc tgcccttcgt ggtatttcag tttctgattt
    37921 ggcataagaa aggggataaa atggctcaaa ttaaaattac tttgactaag tctccaatcg
    37981 gacgcattcc atcacaacgt aaaactgttg tagcacttgg acttggcaaa ttgaacagct
    38041 ctgttattaa agaagataac gctgctatcc gtggtatgat cacagcagta tctcacttag
    38101 taacagttga agaagtaaac tagatatagt catctatgat gacatcgtat aggcgagttg
    38161 atgggggaga caaccttttc tcccttatcg gcgctagcat tttacaaaag aggagaaaat
    38221 aaaaatgaaa cttcatgaat tgaaacctgc agaaggttct cgtaaagtac gtaaccgcgt
    38281 tggtcgtggt acttcatcag gtaacggtaa aacatctggt cgtggtcaaa aaggtcaaaa
    38341 agctcgtagc ggtggcggag ttcgccttgg ttttgaaggt ggacaaactc cattgttccg
    38401 tcgtcttcca aaacgtggat tcactaacat caacgctaaa gaatacgcaa ttgtgaacct
    38461 tgaccaattg aacgtctttg aagatggtgc tgaagtaact ccagttgttc ttatcgaagc
    38521 aggaattgtt aaagctgaaa agtcaggtat taaaattctt ggtaacggtg agttgactaa
    38581 gaaattgact gtgaaagcag ctaaattctc taaatcagct gaagaagcta tcactgctaa
    38641 aggtggttca gtagaagtca tctaagagag gtgacctatg ttttttaaat tattaagaga
    38701 agctcttaaa gtcaagcagg ttcgatcaaa aattttattt acaattttta tcgttttggt
    38761 ctttcgtatc ggaactagca ttacagttcc tggtgtgaat gccaatagct tgaatgcttt
    38821 aagtggatta tccttcttaa acatgttgag cttggtgtcg gggaatgccc taaaaaactt
    38881 ttcgattttt gccctaggag ttagtcccta tatcaccgct tctattgttg tccaactctt
    38941 gcaaatggat attttaccca agtttgtaga gtggggtaaa caaggggaag taggtcgaag
    39001 aaaattgaat caagctactc gttatattgc tctagttctc gcttttgtgc aatctatcgg
    39061 gattacagct ggttttaata ccttggctgg agctcaattg attaaaactg ctttaactcc
    39121 acaagttttt ctgacgattg gtatcatctt aacagctggt agtatgattg tcacttggtt
    39181 gggtgagcaa attacagata agggatacgg aaacggtgtt tccatgatta tctttgccgg
    39241 gattgtttcc tcaattccag agatgattca gggcatctat gtggactact ttgtgaacgt
    39301 cccaagtagc cgtatcactt catctatcat tttcgtaatc attttgatta ttactgtatt
    39361 gttgattatt tactttacaa cttatgttca acaagcagaa tacaaaattc caatccaata
    39421 tactaaggtt gcacaaggtg ctccatctag ctcttacctt ccgttaaagg taaatcctgc
    39481 tggagttatc cctgttatct ttgccagttc gattactgca gcgcctgcgg ctattcttca
    39541 gtttttgagt gccacaggtc atgattgggc ttgggtaagg gtagcacaag agatgttggc
    39601 aactacttct ccaactggta ttgccatgta tgctttgttg attattctct ttacattctt
    39661 ctatacgttt gtacagatta atcctgaaaa agcagcagag agcctacaaa agagtggtgc
    39721 ctatatccat ggagttcgtc ctggtaaagg tacagaagaa tatatgtcta aacttcttcg
    39781 tcgtcttgca actgttggtt ccctcttcct tggtgtgatt tccattttac cgattgcagc
    39841 taaagatgta tttggtcttt ctgatgttgt tgcctttggt ggaacaagtc tcttgatcat
    39901 tatctctaca ggtatcgaag gaatcaagca attggaaggt tacctattga aacgtaagta
    39961 tgttggtttc atggacagaa cagaataaaa gtatttactg aatcagtaaa tactgaggga
    40021 gtggaggttt aaactctgac atttgtaaga gttggatctc ccctcttcta ttttgttttt
    40081 aaatcggggt gaaaaaactt tttgcttcta tttaaaaaca aaataaggag atcaaatcat
    40141 gaatcttttg attatgggct tacctggtgc aggtaaggga actcaagcag caaaaatcgt
    40201 agaacaattc catgttgcac atatctcaac aggtgatatg ttccgcgctg caatggcaaa
    40261 tcaaactgaa atgggtgttc ttgctaagtc atatattgac aagggtgaat tggttcctga
    40321 cgaagttaca aatggaatcg taaaagaacg cctttcacaa gatgatatta aagaaacagg
    40381 attcttattg gatggttacc cacgtacaat tgaacaagct catgccttgg acaaaacatt
    40441 ggctgaactt ggcattgaac tagaaggtat tatcaatatt gaagtgaacc ctgacagcct
    40501 cttggaacgt ttgagtggcc gtatcatcca ccgcgtaact ggagaaactt tccacaaggt
    40561 ctttaaccca ccagttgact ataaagaaga agattactac caacgtgaag atgataagcc
    40621 tgagacagta aaacgtcgtt tggatgttaa tattgctcaa ggagaaccaa tcattgctca
    40681 ctaccgtgcc aaaggtttgg ttcatgacat cgaaggtaat caagatatca atgatgtctt
    40741 ctcagatatt gaaaaagtat tgacaaattt gaaataaagc gtttttcaca cttgcaaaaa
    40801 tccgctacaa atgttatact gaaatagtct gacttataat tgttgtctct gtgtctagag
    40861 gcatcgaatc gaaatttatg gaggtgcttt tgcgtggcaa aagacgatgt gattgaagtt
    40921 gaaggcaaag tagttgatac aatgccgaat gcaatgttta cggttgaact tgaaaatgga
    40981 catcagattt tagcaacagt ttctggtaaa attcgtaaaa actatattcg tattttagcg
    41041 ggagatcgtg ttactgtcga aatgagtcca tatgacttga cacgtggacg tatcacttac
    41101 cgctttaaat aatcgaaaaa cttggaggga taagaaatga aagtaagacc atcggtcaaa
    41161 ccaatttgcg aatactgtaa agttattcgt cgtaatggtc gtgttatggt aatttgccca
    41221 gcaaatccaa aacacaaaca acgtcaagga taagatagaa aggagaaaac atggctcgta
    41281 ttgctggagt tgatattcca aatgacaaac gcgtagtaat ctcattgact tatgtttatg
    41341 gtatcggact tgcaacatct aagaaaattt tggctgctgc tggaatctca gaagatgttc
    41401 gtgtacgtga tcttacatca gatcaagaag atgctatccg tcgtgaagtg gatgcaatca
    41461 aagttgaagg tgaccttcgt cgtgaagtaa acttgaacat caaacgtttg atggaaatcg
    41521 gttcataccg tggtatccgt caccgtcgtg gacttcctgt ccgtggacaa aatactaaaa
    41581 acaacgctcg cactcgtaaa ggtaaagctg ttgcgattgc tggtaagaaa aaataatata
    41641 ggaggtaaaa gtcttggcta aaccaacacg taaacgtcgt gtgaaaaaga atatcgaatc
    41701 tggtattgct catattcacg ctacatttaa taacactatt gttatgatta ctgatgtgca
    41761 tggtaatgca attgcttggt catcagctgg tgctcttggt ttcaaaggtt ctcgtaaatc
    41821 tacaccattc gctgctcaaa tggcttctga agctgctgct aaatctgcac aagaacacgg
    41881 tcttaaatca gttgaagtta ctgtaaaagg tccaggttct ggtcgtgagt cagctattcg
    41941 tgcgcttgct gccgctggtc ttgaagtaac agcaattcgt gatgtgactc cagtgccaca
    42001 caatggtgct cgtcctccaa aacgtcgccg tgtataatca tcgcattaca ctgcttttcg
    42061 tttaagaggg agtaactaaa tgatcgagtt tgaaaaacca aatataacaa aaattgatga
    42121 aaataaagat tatggcaagt ttgtaatcga accacttgaa cgtggctacg gtacaactct
    42181 tggtaactct cttcgtcgtg tacttctagc ttctctacca ggagcagctg tgacatctat
    42241 caacattgat ggtgtgttac atgagtttga cacagttcca ggtgttcgtg aagacgtgat
    42301 gcaaatcatt ctgaacatta aaggaattgc agtgaaatcg tacgttgaag acgaaaaaat
    42361 catcgaactg gatgttgaag gtcctgctga agtaacagct ggtgacattt tgacagatag
    42421 cgatattgaa attgtaaatc cagatcatta tctctttaca atcggtgaag gttcttctct
    42481 aaaagcgact atgactgtta acagtggtcg tggatatgta cctgctgacg aaaataaaaa
    42541 ggataatgca ccagttggaa cacttgctgt agattctatt tatacaccag ttacaaaagt
    42601 caactatcaa gtggaacctg ctcgtgtagg tagcaatgat ggtttcgaca aattaaccct
    42661 tgaaatcttg acaaatggaa caattattcc agaagatgct ttagggcttt cagcacgtat
    42721 tttgacagaa catcttgatt tgtttacaaa tcttactgag attgctaagt caactgaagt
    42781 gatgaaagaa gctgatactg aatctgacga ccgtatttta gatcgtacga ttgaggaact
    42841 ggacttgtct gtgcgttcat acaactgttt aaaacgtgcc ggtatcaata ctgtgcatga
    42901 tttgacagaa aaatctgaag cagagatgat gaaagtacga aatcttggac gcaagagttt
    42961 ggaagaagtg aaactcaaac tcattgattt gggtcttgga ttaaaagata aataaaggag
    43021 gaatacatgg cttaccgtaa actaggacgc actagctcac aacgtaaagc aatgcttcgc
    43081 gatttgacaa ctgacctttt gatcaacgaa tcaatcgtga caactgaagc tcgtgctaaa
    43141 gaaatccgta aaactgttga aaaaatgatt actctaggta aacgtggtga tttgcatgca
    43201 cgtcgtcaag cagctgcttt cgtacgtaat gaaattgcat ctgaaaacta tgatgaagca
    43261 actgataagt acacttctac tacagcactt caaaaattgt tctcagaaat cgcacctcgt
    43321 tatgctgaac gtaacggtgg atacactcgt atccttaaaa ctgaaccacg tcgtggtgat
    43381 gcagcgccaa tggcgatcat cgaattagta taaaatcatc aattttgttg agtgttatga
    43441 tgatggagtc ttgtgctctt agtctagctc tggtctaccg ctaggatttc ggtcctagcg
    43501 ggaacactca tcataagttg ggatagtaga cgcttgttta cgaaattgtt tttttcttaa
    43561 gaacaacttc gtaagcaggc gtttttgagt attttcgtta gaattatgct atactatttg
    43621 aaaagaatcc tgtttaatgt taaggtttct tattttaaga agaattggag tttacttatg
    43681 aaagccatta taactgttgt tggtaaagat aaatctggaa ttgttgcagg tgtttctggt
    43741 aaaattgcag aattaggatt gaatattgac gatatctctc aaactgtctt ggatgaatat
    43801 tttacgatga tggctgttgt atctagtgat gaaaagcaag attttaccta tcttcgtaat
    43861 gaatttgaag cttttgggca aactttgaat gtaaaaatca atattcagag tgcagcgatt
    43921 ttcgaagcta tgtataatat ctaggaggtc atcatggata ttagacaagt tactgaaacc
    43981 atcgccatga ttgaggagca aaacttcgat attagaacca ttaccatggg gatttctctt
    44041 ttggactgta tagatccaga tatcaatcgt gctgcggaga aaatctatca aaaaattacg
    44101 acaaaggcgg ctaatttagt agctgttggt gatgaaattg cggctgagtt gggaattcct
    44161 atcgttaata agcgtgtatc ggtgacacct atttctctga ttggggcagc gacagatgcg
    44221 acggactacg tggttctggc aaaagcgctt gataaggctg cgaaagagat tggtgtggac
    44281 tttattggtg gtttttctgc cttagtacaa aaaggttatc aaaagggaga tgagattctc
    44341 atcaattcca ttcctcgcgc tttgactgag acggataagg tctgctcgtc agtcaatatc
    44401 ggctcaacca agtctggtat taatatgacg gctgtggcag atatgggacg aattatcaag
    44461 gaaacggcaa atctttcaga tatgggagcg gccaagttgg ttgtattcgc taatgctgtt
    44521 gaggacaatc catttatggc gggtgccttt catggtgttg gggaagcaga tgttatcatc
    44581 aatgtcggag tttctggtcc tggtgtggtg aaacgtgctt tggaaaaagt tcgtggacag
    44641 agctttgatg tagtagccga aacagttaag aaaactgcct ttaaaatcac tcgtatcggt
    44701 caattggttg gtcaaatggc cagtgagaga ctgggtgtgg agtttggtat tgtggacttg
    44761 agtttggcac caacccctgc ggttggagac tctgtggcac gtgtccttga ggaaatgggg
    44821 ctagaaacag ttggcacgca tggaacgacg gctgccttgg ccctcttgaa cgaccaagtt
    44881 aaaaagggtg gagtgatggc ctgcaaccaa gtcggtggtt tatctggcgc ctttatccct
    44941 gtttctgagg atgaaggaat gattgctgca gtgcaaaatg gctctcttaa tttagaaaaa
    45001 ctagaagcta tgacggctat ctgttctgtt ggattggata tgattgccat cccagaagat
    45061 acgcctgctg aaaccattgc ggctatgatt gcggatgaag cagcaatcgg tgttatcaac
    45121 atgaaaacaa cagctgttcg tatcattccc aaaggaagag aaggcgatat gattgagttt
    45181 ggtggtctat taggaactgc acctgttatg aaggttaatg gggcttcgtc tgtcgacttc
    45241 atctctcgcg gtggacaaat cccagcacca attcatagtt ttaaaaatta agaaaatagg
    45301 agaaatttta agttctattt aagattagat gtgtatacta taatcattaa ataaagacct
    45361 cctaatatta tttgaaacag ataacactga attagtttga atttgatttt catctaatat
    45421 ctttatttaa tgaactccta aactttttca taataatctc cttcaaaagt cgcctgtatg
    45481 ggtggctttt attttatcat tcatgatata atagaagcaa acggaggacg gaaaatggta
    45541 aaagtacgat tgtatttggt acgtcatggc aagaccatgt ttaacacgat tggtcgcgcg
    45601 caaggttgga gcgatactcc cttaactgct gaaggtgaac gagggattca agagttagga
    45661 atcggtttgc gagaatctga tctacagttt gagcgcgctt attcgagtga ttctggtcgt
    45721 accattcaga ccatgggaat tatccttgaa gaacttggct tgcaggggaa aatcccttat
    45781 cgcatggaca agcgtatcag agaatggtgt ttcggtagtt ttgatggagc ctatgatggc
    45841 gatcttttta tgggcattat tcctcgtatc tttaatgtgg accacgttca ccaattgtct
    45901 tatgctgaac tggctgaggg cttggtagag gtcgatacag ctggttgggc tgaaggctgg
    45961 gaaaaactca gtggccgaat caaggaaggc tttgaaatga ttgcaaaaga aatggaagat
    46021 caaggtggag gtaacgccct tgttgtcagc catggaatga ctattggaac cattgtttat
    46081 ctgattaatg gcatgcatcc gcatggtcta gataatggta gcgtgacgat tcttgaatat
    46141 gaggatggtc agtttagggt tgaagttgtc ggtgaccgta gttaccgaga gctaggacgt
    46201 gagaagatgg aagaaggctc tatttaatca gtctagactt gcttgccatg agctagggat
    46261 ttgataagaa tatcaagata agaaaaaaca gccgagggta ctcctttcgg ctgtttttga
    46321 tgtggaaaac taaagtgtaa tgctattgct tttagagatt ttcataaaca agagcaagga
    46381 acctactgtt agaacagtca ggatagttga caaggctgcg gctacaccat aatttcctct
    46441 gagaacctct gtataaatag ctacagtcat tgttcttgtt ttgacattgt agaggaggat
    46501 agaagtagag agttttgaaa tcattgtgac ccaagataag atggctccag aaatgatacc
    46561 agatagcatc attggagttg taatcttagc aaaggtattg agacgactac ttcctaagct
    46621 ttcagcagct tcttcaatac ttggtgctat ttgttgtaag ctagcaacag atgagcgaat
    46681 agtataaggt aatcttctga cagatagaga cataatcaag atgaaagcag tccctgtaat
    46741 cataagaaat ccacttccaa atagaccaga gaaatgaagg caatccctag aacggttcct
    46801 ggtacaatat aaggtaccat actgaggctg tcaattaagt ttgtaaacaa attccgtttt
    46861 ctaacggcta ggtaggagat aaatgtcgca aatagaacaa ctagaactaa ggcaatcaaa
    46921 gggatacgaa tggtattgaa aatagcagat cccatacgat tgaaagctag cttgtaactg
    46981 tttggagaat aacctttaac aaataccata cctgatgttt ttaggaaaga ggtataaatt
    47041 aagtagattt gaggtaaaac agagataaag ataattccgt agactgttgc ataaatggca
    47101 gccatttttc cttttgtagt ttttttaggc tcaattggat ggagcagatt catgctgaaa
    47161 ctgtagcggt ttgcaatgtg tttttggata aggaaaattg ccaaggcaat gataatcgcc
    47221 ataattgcaa aagcagaatt tcctccaacc tcgctaataa attgggtata aatcaggaca
    47281 gggaaagtcc gatatccttc gccaatcaac ataggcgttc caaagtctga gaatgctctc
    47341 ataaatacaa gcaaggagct gctagtaagg ttggaactag gagaggtaaa acaaccgtta
    47401 cgataggttt aaatccgaag gaccccatgc tttcagctgc ttcaagtaga gaattgtcaa
    47461 tactgttcat tgttccagca acatatagaa ataccagtgg gaatagttgc agtgtaaaga
    47521 caagtacaat tcctttgaat ccataaatat cgatagctgg aagatgaagg gcatttgtca
    47581 aaaatttagt gatgacctca tttcgtctca gcaagagaat ccaggagtag gctcctacga
    47641 aaggagttga catggaagca atgataatca atatttgtag aaatttcttc cccttgaagt
    47701 catacataga gaagagataa gctaataggg ttcctacaac taaggaagtg acagtagcgg
    47761 taatggaaac cctgaaactg ttgactagtg tctcagagta gtaggtttta ctaaagaaag
    47821 tgacaaaatt agctagtgag aattatcctt catgtataag tgcttacttg agcacggtaa
    47881 cgataggata aacgagaaag acaagatagg taagaaagat gaagaaagag gaggctgtcc
    47941 aaatatttag ttttttacgt tccatggttg actcctttta tcaggttttg ggaaccatct
    48001 gcagaaaaga tgtttaattt ttgcgtattg attcgtagac gaatacgatt gtctttttgt
    48061 agatcttctt caaaagttga ttcttcacta acttgaattt ttgaggcaaa acctgtctca
    48121 atgaaatact ccgtatttag tccaagatag acgctatcgc taatagttcc ttcgatatct
    48181 ccagattcat ctttgataaa ctcttcggga cgaatgctta catgaatagc ttgctcctca
    48241 acctgatcaa gagctagcat tcgaagggca tagccatctg aaaagacgat ataagcgccg
    48301 tcgctccgtt tttcaagatt ggcagggata atatttgtgc gtccgataaa ggttgccaca
    48361 aactcattag ctggtttatg atagagttct tttggtcggc cgatttgttg gatcacccca
    48421 tctttcataa cagcaatttg gtctgaaata gccatggctt cttcttggtc gtgggttaca
    48481 taaacagttg taattcccac ttcgtgttgg atttctcgga tggtttgacg catatccaag
    48541 cgaagtttgg cctccagatt actaagtggc tcgtccatga ggagaacact tggattaacc
    48601 gctaaggcgc atgccaaggt gacacgttgt tgttgtccac cactgacttt atcgggcttt
    48661 caatccgcat attgagcaat ttgcatgagt tcaagatact tgttggtctg ttgaatcaat
    48721 tcttcttttg gaaccttctt ttgcataaga ccaaaagcaa cgttgtctcg gacagtcaaa
    48781 tgtgggaaaa tagcgtagtt ttggaaaacc atcccgatat tgcgtttgct gggttccata
    48841 ttattgattt ttgtatcatc gaagtaaaat tctccatctt tgatactgtt gaaacctgca
    48901 atcatacgaa gaagggtcgt tttcccacat cctgaagctc caagaagggt aaagagactt
    48961 ccttttggaa ttgtaatgtt caaattctca ataacaggga catcgtggta gatttttttg
    49021 gcgttaataa tttcgatctc actcatagtg aacctctttt actgtttaga ttggatatct
    49081 gtaaagactt cgttgtattt cttaacgata tctgatttat tcttgatgac ataatcataa
    49141 tcttcagtga gtgttttgat tttgtcaatt ggtttcatgt tttcgcttgt tttagcattt
    49201 ttacgaacag gacggttagt agtggttgta ccaagtgtat cttgtacttc ttgagagata
    49261 ataaaatcga taaatttctt ggcattttcc atatttttag cttttttaac gatagcagca
    49321 ctagcaggta ggaagacggt tccttctttt ggatagacta ccttaatgtt agctccgtca
    49381 tttaagagtt taactgctgg atcctcataa gagagaccaa cagccatttc tccatcagcg
    49441 actgctttat agacaccaga tgaacttaaa ccgattttat agtatattga aactagaata
    49501 gtacacatct acttctaaaa cattgttaga aatcgatttg actgtcctga ttaatttgtc
    49561 ctgttcttat ttcattttac tataccatca ataagtgtga aaagatcttt tacataagac
    49621 caagccttat catctttgta accaccttga gcttgtagca tatttgttaa ttgagcaaag
    49681 gcgctagaag agtttgctgg gtcggcagtt gcgatttttc cttttagttc aggtttgaga
    49741 agatcgttat atccttcgat gttcatgcct ttagttaaat cagggttgac gattaaaaca
    49801 ctaccatcta gtgtataagg agtagagtag ccagttgtgt tttgatattc tttgataaca
    49861 ttatcatttt cttttgaagt atagttttca aagagttctc cgtgggtagc atattgtgta
    49921 taagaaccac caaagataac atcagctaca ggaacttctt tttctgactc tagttttttg
    49981 aaaagttctc cagtaccagc ttgaatcagt tctactttga taccatattt ttcttcaaag
    50041 gcaggaatag ttgctccaat taagtcctct gagtttggtc aataaacgac tagcgaacca
    50101 ccgtctcctt tatcagatga actgtcatcg gcagattcat tagaagaaca agcagcataa
    50161 tacatccatt tctttttcat gatgggatat ctccgttgtg ttatttaagt ttattttaaa
    50221 acctacaatt ctattc
//
LOCUS       DAVGJL010000002        49992 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.002, whole genome shotgun sequence.
ACCESSION   DAVGJL010000002 DAVGJL010000000
VERSION     DAVGJL010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 49992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49992
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.002"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(<1..392)
                     /locus_tag="VVE42_000065"
     CDS             complement(<1..392)
                     /locus_tag="VVE42_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU3072340.1"
                     /translation="MNTLAEKFRLKRKELRLSQQTLAEGICEQSQISKIERGHFIPSA
                     DLLFKLSQRLEVPLDYFFNEQIEIKSNLSNFKQLSARLLDDRNYDDLEYIYRIEIERS
                     TFLTLEDRTYLEWIKAIIDFYQYDSKCEA"
     gene            896..2911
                     /locus_tag="VVE42_000066"
     CDS             896..2911
                     /locus_tag="VVE42_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU3072341.1"
                     /translation="MKKLFILISNLLASLFFVWVFTIWTDTYVSHYYPNVVVHDSSPE
                     TTFQHVATRLEKLAEETDSFIAIQHQDPNSEGTTVFSYTTFGDGKLPDGLQEKKLEDA
                     QSSSVETNYFVFDGHLDIHLLREELSQLGLTNMNLTIPSKLSTLMAIFSNGFQLISLL
                     IFILTFVALTLISQISQLRSSGIRLISGEKRWSIFLRPVGEDLKGIAVGFSLAGVLAI
                     LMQKILSLPTQSLMTIGEGLLSYNLILLSISLFFAQLFAVGIKKIHLMQIIKGQVPVR
                     GIISLILIGQLLAIIIVTLGIGSSLKYSQAWQQHRIGQEAWSQERQLITLSISREGTS
                     PGFDEQAQRKLRTWYQLMDLAVSEQKAFLSRHQLIDRTLQNGMASSKNLITSTEWHDY
                     NPNGNVLIVTPQYLERQNIPVDTTIEQKMNHLNVGEFVLLLPEHLRSEEEHYKSVFED
                     DLTSRMSSQDERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQ
                     SILFWIDAVQNYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVM
                     LAGAVLGIATSILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLG
                     FVASVFLQVEIGVAFLVLLLFTGLSLLQLHVQMQKENKMSILVLKGG"
     gene            2913..3545
                     /locus_tag="VVE42_000067"
     CDS             2913..3545
                     /locus_tag="VVE42_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3072342.1"
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLM
                     NMIGKLEPYDGTIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLIG
                     QKLSRSEQRLRQKQALEQVGLAYLDLDKRIFELSGGESQRVALAKIILKNPPFILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVFTMDHLK"
     gene            complement(4473..4886)
                     /gene="ndk"
                     /locus_tag="VVE42_000068"
     CDS             complement(4473..4886)
                     /gene="ndk"
                     /locus_tag="VVE42_000068"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEU3072343.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIDQHYQDLVGQSFYLPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEEALPGTI
                     RGDFAKAAGENEVIQNVVHGSDSEESAKREIALWF"
     gene            complement(4997..8674)
                     /gene="rpoC"
                     /locus_tag="VVE42_000069"
     CDS             complement(4997..8674)
                     /gene="rpoC"
                     /locus_tag="VVE42_000069"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEU3072344.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMAYRNGYVHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKIL
                     FNDIMPEGLPYLQEPNNANLTEGVPAKYFLPLGGDIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKAEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGQVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPHAVNEEEYLNPPVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            complement(8707..12318)
                     /gene="rpoB"
                     /locus_tag="VVE42_000070"
     CDS             complement(8707..12318)
                     /gene="rpoB"
                     /locus_tag="VVE42_000070"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEU3072345.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFTDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVVAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSRLNEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
     gene            12734..12804
                     /locus_tag="VVE42_000071"
     tRNA            12734..12804
                     /locus_tag="VVE42_000071"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12766..12768,aa:Cys,seq:gca)
     gene            complement(13002..14333)
                     /locus_tag="VVE42_000072"
     CDS             complement(13002..14333)
                     /locus_tag="VVE42_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEU3072346.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTDTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRTAPVIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVHQIGEDTYIVQGTMTLNDFNNYFDVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELVSQNKQFILTNDKVKNGRVTKVKVQITEVEIEEETE"
     gene            complement(14624..15448)
                     /gene="endA"
                     /locus_tag="VVE42_000073"
     CDS             complement(14624..15448)
                     /gene="endA"
                     /locus_tag="VVE42_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HEU3072347.1"
                     /translation="MNKKTRQTLIGLLVLLLLSAGSYYIKQNELGNQAAKTKMSQKKQ
                     APEAPSQELAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            complement(15487..15675)
                     /locus_tag="VVE42_000074"
     CDS             complement(15487..15675)
                     /locus_tag="VVE42_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEU3072348.1"
                     /translation="MNKKSGYVIKRLLLVILVLILGLLALGIGLMVGYGILGKGQDPW
                     AILSPAKWQELIHKFTGN"
     gene            complement(15668..16951)
                     /gene="murA"
                     /locus_tag="VVE42_000075"
     CDS             complement(15668..16951)
                     /gene="murA"
                     /locus_tag="VVE42_000075"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEU3072349.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEANDEDE"
     gene            complement(16975..17205)
                     /locus_tag="VVE42_000076"
     CDS             complement(16975..17205)
                     /locus_tag="VVE42_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEU3072350.1"
                     /translation="MVQLLFTLSSHMLFIYVSFYLLKDLVRWEKVLKVTDDNTRKVRL
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            complement(17263..18300)
                     /locus_tag="VVE42_000077"
     CDS             complement(17263..18300)
                     /locus_tag="VVE42_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEU3072351.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     VNDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKDIELKYFGVYVLNVTDNSTFKGILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFAPDNPVSEEEQKAHPDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            complement(18284..18772)
                     /gene="coaD"
                     /locus_tag="VVE42_000078"
     CDS             complement(18284..18772)
                     /gene="coaD"
                     /locus_tag="VVE42_000078"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEU3072352.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDMIERASRLFDKLYVGIFFNPHKQ
                     GFLPLENRKRGLEKALGHLENVEVVASHDKLVVDVAKRLEATFLVRGLRNAADLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGQDIACHVPESILEEIRNEK
                     KD"
     gene            complement(18762..19301)
                     /gene="rsmD"
                     /locus_tag="VVE42_000079"
     CDS             complement(18762..19301)
                     /gene="rsmD"
                     /locus_tag="VVE42_000079"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEU3072353.1"
                     /translation="MKIVSGIYGGRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEVGR
                     VLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQMTKEVGKFQLLKMDAER
                     ALEQVSGEFDLVFLDPPYAKEQIVADIEKMAERELFSEDVMVVCETDKAVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            complement(19366..20358)
                     /locus_tag="VVE42_000080"
     CDS             complement(19366..20358)
                     /locus_tag="VVE42_000080"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEU3072354.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTPKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            complement(20470..21153)
                     /locus_tag="VVE42_000081"
     CDS             complement(20470..21153)
                     /locus_tag="VVE42_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEU3072355.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYFLMQGRLWLTIATFAELALVFVASSMASRNSPAALPVFLLYSVLNGFTLGFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            complement(21209..21949)
                     /locus_tag="VVE42_000082"
     CDS             complement(21209..21949)
                     /locus_tag="VVE42_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEU3072356.1"
                     /translation="MTIITSKANSVVKNAKKLHQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFALENYRNQLAAFPQTVWISEDILLDLADSQTPQGIVAVVQKEEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSQALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVAAGILIFHLS"
     gene            21983..22261
                     /locus_tag="VVE42_000083"
     CDS             21983..22261
                     /locus_tag="VVE42_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEU3072357.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYPDFKIAN"
     gene            22339..23265
                     /gene="yidC"
                     /locus_tag="VVE42_000084"
     CDS             22339..23265
                     /gene="yidC"
                     /locus_tag="VVE42_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEU3072358.1"
                     /translation="MKSIKRFALSAMGVAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPILLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVREELAKNPSKASAFST
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(23340..24134)
                     /gene="pflA"
                     /locus_tag="VVE42_000085"
     CDS             complement(23340..24134)
                     /gene="pflA"
                     /locus_tag="VVE42_000085"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEU3072359.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTSQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKQLMDTESYQDYMKRVHG"
     gene            complement(24260..25510)
                     /locus_tag="VVE42_000086"
     CDS             complement(24260..25510)
                     /locus_tag="VVE42_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEU3072360.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERAAGIPEIISCRYNPGGVFELGTDIMDNPGEAK
                     FGMTKDQLFEAFAILKEKGAKTFGIHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     ISLDFINLSGGIGVNYRPDQEPNDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEAVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGK
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            complement(25577..26404)
                     /gene="purR"
                     /locus_tag="VVE42_000087"
     CDS             complement(25577..26404)
                     /gene="purR"
                     /locus_tag="VVE42_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEU3072361.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLREFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK"
     gene            complement(26671..27612)
                     /locus_tag="VVE42_000088"
     CDS             complement(26671..27612)
                     /locus_tag="VVE42_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEU3072362.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVVHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMSNKIFAMDNRSFYKPDLD"
     gene            complement(27602..28858)
                     /locus_tag="VVE42_000089"
     CDS             complement(27602..28858)
                     /locus_tag="VVE42_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEU3072363.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVAVSDRLQEVRIELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEEAYETGDKDEIERCRKSLLASVKRFAKDIKSKYI
                     APPRTTNFGILFVPTEGLYSEIVRNPVFFDDLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLHFQENEEEYED"
     gene            complement(28860..29522)
                     /locus_tag="VVE42_000090"
     CDS             complement(28860..29522)
                     /locus_tag="VVE42_000090"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEU3072364.1"
                     /translation="MSEYKLSENNWTRVAVFAGGNRGHYRTDFDAFVGVDRGSLWVLE
                     EDLPLALAVGDFDSVTEEERQVIQKRAQYFVQARPEKDDTDLELALLTIFEQNPQAQV
                     TIFGALGGRIDHMLANVFLPSNPKLAPYMRQIEIEDGQNLIAYCSEGTSQLEPRSDYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            complement(29485..30141)
                     /locus_tag="VVE42_000091"
     CDS             complement(29485..30141)
                     /locus_tag="VVE42_000091"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEU3072365.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDSHFVP
                     QISFGAGVVESLRPHSKMVFDCHLMVSNPEHHLEDFARAGADIISIHVEATPHIHGAL
                     QKIRSLGVKPSVVINPGTPVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALREEKGLNFEIEVDGGIDDQTIAQAKEAGATVFVAGSYVFKGEVNERVQTLRKQLD
                     "
     gene            complement(30152..31030)
                     /gene="rsgA"
                     /locus_tag="VVE42_000092"
     CDS             complement(30152..31030)
                     /gene="rsgA"
                     /locus_tag="VVE42_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEU3072366.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDRGELDFYQQTYGDIGYDFVTSKEELLSLLTGKVTVFMGQTGV
                     GKSTLLNKIAPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVS
                     RAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGVIATFRFDNYLQFLSEI
                     ENRRETYKKVSKKIPK"
     gene            complement(31032..31904)
                     /gene="rsmA"
                     /locus_tag="VVE42_000093"
     CDS             complement(31032..31904)
                     /gene="rsmA"
                     /locus_tag="VVE42_000093"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEU3072367.1"
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRLVPILVDTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPNLPIKVVANLPYYITTPILMHLIESGIPFCEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLA
                     EFAGLADALKGQGL"
     gene            complement(32035..32343)
                     /locus_tag="VVE42_000094"
     CDS             complement(32035..32343)
                     /locus_tag="VVE42_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3072368.1"
                     /translation="MCEKIRIRRVSDYPSARGGLEDILIMENMTNHLLLVQIRVHGYL
                     LDFASIEGQRQKHYRLKNLPQTVELTVDDVEEDVDLTLPENRSYQEADFFERMFRENC
                     "
     gene            complement(32336..32977)
                     /locus_tag="VVE42_000095"
     CDS             complement(32336..32977)
                     /locus_tag="VVE42_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3072369.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            complement(32979..35009)
                     /locus_tag="VVE42_000096"
     CDS             complement(32979..35009)
                     /locus_tag="VVE42_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU3072370.1"
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMTN
                     QPVASREEVTEALSHLAVEHNSLIARRIVEPNEAGETRFTYATYGEGELPEGLTISSK
                     ESAETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLAATPMVLL
                     SLAIFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGIALRPVLEDVRQLICSVLVSSL
                     LGLGILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVYLLGLQENSLVDLLKGK
                     LPLKRMMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQSSDRYRLSFGW
                     SSAFADEEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYTP
                     SGNVIYVSPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSVYYQGLFTDYL
                     QNFSSESVEVTSQKHYLPQVRLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQAT
                     GTRPVAGMLWGTTANSALKLDRYGDSITALKEQGLYHKVSYLVKSQLFFAKVLNDKRV
                     EFYSLLIGTILTLSTAILLFDSMNLLYFEQFRRELMIKRLAGMTIYELHGKYLLAQGG
                     VLLLGLVLSSILTRDGLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            complement(35086..35382)
                     /locus_tag="VVE42_000097"
     CDS             complement(35086..35382)
                     /locus_tag="VVE42_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEU3072371.1"
                     /translation="MKQTVKKLALVASIAATLGGSVAVASAAVQYPEGGVWTYGSGNG
                     GAYSNYYHPSKYHSSTVVSRKTGSSDKGYAGAGGTSRAWIRTSWGEKVAFYYNV"
     gene            complement(35702..36601)
                     /locus_tag="VVE42_000098"
     CDS             complement(35702..36601)
                     /locus_tag="VVE42_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU3072372.1"
                     /translation="MLIGQKIKEIRIEKGVSRPDFCGDEQELTVRQLSRIESGASQPS
                     LPKLDYIARRLGVPVYSLMPDFSALPSAYLELKYQILREPIYGKEEEYDKKEACLEEI
                     YKTYFDNLPKEEQLACEVLQACLDTSRTRRPEYAELILEEHMPQIIEKEAYSINDMLL
                     IRLFFYQMLIRKDLAKFINQIEKLMLFLLEQKKVTKLENFFIIRDTLISGMCCLEKVG
                     VTDCFNDYLSCLQEIMDKTQDYQKKPLVFMFLWKQALREERDFSLAESFYQSSKTFAQ
                     LIGDEFLVKKLTEEWQEDVKKYL"
     gene            complement(36673..37233)
                     /gene="rnmV"
                     /locus_tag="VVE42_000099"
     CDS             complement(36673..37233)
                     /gene="rnmV"
                     /locus_tag="VVE42_000099"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEU3072373.1"
                     /translation="MKEKISQVIVVEGRDDTVNLKRYFDVETYETRGSAINDQDIERI
                     QRLHQRHGVIVFTDPDFNGERIRRMIMTAIPTVQHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            complement(37233..38006)
                     /locus_tag="VVE42_000100"
     CDS             complement(37233..38006)
                     /locus_tag="VVE42_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEU3072374.1"
                     /translation="MIFDTHTHLNVEEFAGREAEEIALADEMGVTQMNIVGFDKPTIE
                     HALELVDEYEQLYATIGWHPTEAGTYTEEVEAYLLDKLKHSKVVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMTISFSGVVTFKKATDLQEAAKELPLDKMLVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIADLRGMTTEELAAATTANAERIFGLDSK"
     gene            complement(38311..38976)
                     /locus_tag="VVE42_000101"
                     /pseudo
     CDS             complement(38311..38976)
                     /locus_tag="VVE42_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153795.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
     gene            complement(39239..39373)
                     /gene="rpmH"
                     /locus_tag="VVE42_000102"
     CDS             complement(39239..39373)
                     /gene="rpmH"
                     /locus_tag="VVE42_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEU3072375.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
     gene            complement(39518..40732)
                     /locus_tag="VVE42_000103"
     CDS             complement(39518..40732)
                     /locus_tag="VVE42_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU3072376.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSDSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKELLLEIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            complement(40888..41181)
                     /locus_tag="VVE42_000104"
     CDS             complement(40888..41181)
                     /locus_tag="VVE42_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3072377.1"
                     /translation="MMKNSFQKSNFLYYGIILVSVLVEVIMIWQLESLVPLLYPDFIG
                     FLVFHVLYHLILFLVAKRSGRWDYLMIWGLFLMFNLLYDSFLGLLFLGLSFGM"
     gene            41343..41795
                     /locus_tag="VVE42_000105"
     CDS             41343..41795
                     /locus_tag="VVE42_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU3072378.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIAHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            complement(41830..43218)
                     /locus_tag="VVE42_000106"
     CDS             complement(41830..43218)
                     /locus_tag="VVE42_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU3072379.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKVVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     AASPSEEDFGQAVSQLHQALDKAAGKVAKKTPAQQDLIGQVDALIDTLYFAYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELQRQL
                     KAYERHKERNKS"
     gene            43284..44246
                     /locus_tag="VVE42_000107"
     CDS             43284..44246
                     /locus_tag="VVE42_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEU3072380.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLIVMNDEIHAAKYVTKTH
                     TTNVSTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAKKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKDYMEG"
     gene            complement(44275..44613)
                     /locus_tag="VVE42_000108"
     CDS             complement(44275..44613)
                     /locus_tag="VVE42_000108"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3072381.1"
                     /translation="MNTIERTRRLVKGCATHCFEVVDRTDEVSSKHCFEVADRTDEVS
                     SKHVFEVVDETNEVSSKHCFEVADETDEVSSKHCFEVADETDEVSSKHVFEVVDETDE
                     VSNHTYGKAT"
     gene            complement(44677..45687)
                     /gene="ccpA"
                     /locus_tag="VVE42_000109"
     CDS             complement(44677..45687)
                     /gene="ccpA"
                     /locus_tag="VVE42_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEU3072382.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLAKENERIAFVSGPLVDDINGKVRLVGYKETLKKAGITYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
     gene            complement(46112..46711)
                     /locus_tag="VVE42_000110"
     CDS             complement(46112..46711)
                     /locus_tag="VVE42_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU3072383.1"
                     /translation="MKVLVAEDQSMLRDAMCQLLTLQPDVESVLQAKNGQEAIQLLEK
                     ESVDIAILDVEMPVKTGLEVLEWIRSERLETKVVVVTTFKRAGYFERAVKAGVDAYVL
                     KERSIADLMQTLHTVLEGRKEYSPELMEVVMTRPNPLTEQEIAVLKGIARGLSNQEIA
                     DQLYLSNGTIRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            complement(46713..47810)
                     /locus_tag="VVE42_000111"
     CDS             complement(46713..47810)
                     /locus_tag="VVE42_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU3072384.1"
                     /translation="MLERLKSIHYMFWASLIFMLFPILPVVIGELPAWHLLVDILFVV
                     TYLGVLITKNQRLSWLFWGIMLVYVAGNTAFVAVNYIWFFFFLSNLLSYHFGVRSLKS
                     LHVWTFLLAQVLVVGRLLIFQRIEVEFLVYMLGILTFVDLMTLGSVRIRLVEDLKEAQ
                     VKQNTQINLLLAENERNRIGQDLHDSLGHTFAMLSVKTDLALQLFQMQAYPQVEKELR
                     EIQQISKESMCEVRTIVENLKSRTLTSELETVKKMLEIAGIEVETDNQLDTASLTQEL
                     DSMASMILLELVTNIIKHAKASKAYLKLERTEKELILTVSDDGCGFAFLKGDELHTVR
                     DRVFPFSGEVSVISQKHPTEVQVRLPYKERN"
     gene            complement(47810..48547)
                     /locus_tag="VVE42_000112"
     CDS             complement(47810..48547)
                     /locus_tag="VVE42_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU3072385.1"
                     /translation="MKQWIALNKIEFLLTKRQSIYYLLSVGMPTAFYLFFSGMYQNTP
                     GGPANFMRDYLISMTAFSMMSTAIFSFPAVLHTDKINNWQKTLRHSPVNMVEYYLSKI
                     TSMLVDYMVSILVVFSVGHFVRGVDMSLGNWIGAALLLILGSIAFVAIGLTLTLLPTS
                     QLMSVVGNLLYLGLAVLGGLWMPVSLFSDWMQAVGKCLPTYQLMELLKTFLNEGGINL
                     SATVYLLVFSAVLFGLTIYLQGHKENA"
     gene            complement(48549..49433)
                     /locus_tag="VVE42_000113"
     CDS             complement(48549..49433)
                     /locus_tag="VVE42_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3072386.1"
                     /translation="MTVVKVEKLSKKIKDKEILRNISFEINDGECVALIGPNGAGKTT
                     LLDCLLGDKLVTSGQVSIQGLPVTSSKLDYTRAYLPQENVIVQKLKVKELIAFFQRIY
                     PNPLSNQEIDQLLQFVKQQKEQLAEKLSGGQKRLFSFVLTLIGRPKIVFLDEPTVSMD
                     TSTRQRFWEIVQELKAQGVTILYSSHYIEEVEHTADRILLLNKGELIRDTTPLAMRSE
                     EIEKHFILPIAYKEVVEQSNLVENWTLKQDSLQVVTREADAFWELLAQAGCRMQEIEV
                     NNRSLLDTIFEETQKGDD"
     gene            complement(49420..49614)
                     /locus_tag="VVE42_000114"
     CDS             complement(49420..49614)
                     /locus_tag="VVE42_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3072387.1"
                     /translation="MKRGIIYFFIGLSLLVWLVEMFTDWFDQALLRQFIHGALGFGFM
                     IFVVFPMGMEWLKGESHDCG"
     gene            complement(49611..49817)
                     /locus_tag="VVE42_000115"
     CDS             complement(49611..49817)
                     /locus_tag="VVE42_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3072388.1"
                     /translation="MGEEEMRNKMIIAMSLVVAGVMTYLMFSGLDEDFYHFPWKVFAG
                     FGIMSWLVREGLKLVRDVKKEFEE"
BASE COUNT        14391 a        11411 c         9525 g        14665 t
ORIGIN      
        1 gcctcacact tactgtcata ttgatagaag tcaataatag ctttaatcca ttcaaggtaa
       61 gttcggtctt ctagtgttag aaaagtactt cgttcaatct ctattctata aatatattcc
      121 aaatcgtcat aatttctatc gtctaatagt cgagcagaca attgcttgaa attagagagg
      181 ttagatttaa tttcaatttg ttcattaaaa aaataatcta atggtacttc aagtcgttga
      241 gagagtttga acaaaaggtc tgcggaggga atgaaatgcc ctctctcaat tttactaatc
      301 tgactttgtt cacaaattcc ttctgcaaga gtttgttggg agagtctcaa ctcttttctt
      361 ttcaatctga atttctctgc gagtgtattc attaaagata ataaaccttc ctatttgctt
      421 aattgcatta taaaattttt agttcaaaat agcaagttaa aggaataaat aattctttat
      481 ggtaataaac taaaaaaagt caaaaattac ataaaaatgc ttggctaaat aaaatatatc
      541 tgttaaaata aggataatga aaaagaaaag ggtttcacga atgaagaaaa atcgtggaat
      601 tcaaaaatta gctatattag tattactagg tgtttttatg tttagtaata caattcctta
      661 ccaacagttt attcagaaga atagacaatt ggagattcga gtgcaatcgc aaaagaagtc
      721 caatggtctt gatgttggga aggctgattg atttcgtatt actttaaaat tcaactattt
      781 agagaaaggt attctgatga gagcttgtaa caataccaga tagatattag taagcttgtt
      841 agtttataga gggcaggggt atttctgccc tctgttttag aataaggaat gcactatgaa
      901 aaaattattt atactcatat ccaacctgct tgctagtttg ttctttgttt gggtatttac
      961 catttggact gatacatatg tctcccatta ttaccccaac gttgttgtac atgattcttc
     1021 ccctgaaaca acttttcagc atgttgctac acgcttggaa aaactagcag aagaaacaga
     1081 tagctttatt gcgattcaac accaagatcc taactcagaa ggtactacag ttttttctta
     1141 tacgaccttt ggggacggaa agttgcctga tgggcttcag gaaaaaaaac tagaagatgc
     1201 tcaaagtagt agtgttgaaa caaactattt tgtattcgat ggacacctag atattcactt
     1261 gctaagggag gagctaagtc aacttggctt gactaacatg aacctgacaa ttccatctaa
     1321 actatctacg ttaatggcta tctttagtaa cggatttcag ttaatcagtc tattgatttt
     1381 catcttaacc tttgtagcac tgactttaat tagtcaaatt agccaattac ggtcgtcggg
     1441 cattcgcctc atttcaggag agaaacggtg gtccattttc cttagaccag ttggtgagga
     1501 tttgaaaggg atagctgttg gtttcagttt agctggcgtg ctcgccattc ttatgcaaaa
     1561 aattctgtcg cttccaacgc aatccctaat gaccatagga gaaggcttgc tcagttataa
     1621 tctcatcttg ttgtcgatct ccctgttttt cgctcaactc ttcgcagttg ggataaaaaa
     1681 gatacacctg atgcagatta taaaagggca agtgcctgtc agaggaatca ttagtttgat
     1741 tttaatcggt caactattag cgattattat tgtaacgctg ggaataggga gtagcttaaa
     1801 gtattcccaa gcctggcaac agcatcggat tggacaagag gcttggagtc aggaaaggca
     1861 actgattacc ctatcaatca gccgtgaagg aacgagtcct ggttttgatg aacaagctca
     1921 aaggaaactc agaacttggt atcaattgat ggatctggct gtttcagaac aaaaggcttt
     1981 cttatctaga caccagttaa ttgaccgtac tttgcaaaat ggcatggctt cctccaaaaa
     2041 cttgataacc tctacagaat ggcacgatta caacccgaat ggcaatgtcc ttatcgtcac
     2101 accgcaatac ttggagcgtc aaaacattcc tgtagataca actattgagc aaaagatgaa
     2161 tcaccttaat gtaggggagt ttgtcttatt gctacctgaa cacctccgtt cagaggaaga
     2221 acattataaa tctgtttttg aagacgactt aaccagtcgc atgtctagtc aagatgaacg
     2281 acagcaaatg actgctacgg taggttattt agaatcaggt caggatcgtt ttgtgtataa
     2341 tacgacccct atttcttacc agcagttttt gaaagatcca atcatcattg ttataacacc
     2401 ccaatcaact ggtccacagt ccattttgtt ttggatagac gcagtacaga actacgttct
     2461 ctttaatcaa ttgtctgatg cccaggagct tatccagaga caaggcattg aaaattgggt
     2521 ctcagaaatg caaacaggtt accacaacta catcacatta ttggataata tccagaggga
     2581 acgttgggta atgctagcag gagctgtgct tgggattgca acttcaatct tgttgtttaa
     2641 cactatgaat aggctctact ttgaagaatt tagacgtgcc atttttatca aacgcattgc
     2701 aggtctcagg ttcttagaaa tccatcgcac ttatctcttt gctcaactgg gtgtgttttt
     2761 actgggattt gttgcgagtg tatttcttca ggtagagata ggagttgctt tcttagtctt
     2821 gttactcttt actggtctat ctcttttaca gttacatgtc caaatgcaga aagaaaacaa
     2881 gatgtccatt cttgttttga agggaggtta atatgattga acttaaacag gtgagtaaat
     2941 cttttggaga acgagagtta ttttcgaatc tttcaatgac atttgaggct ggaaaagtct
     3001 atgccttaat tggttcaagt ggtagcggaa aaacaacctt gatgaacatg attgggaaat
     3061 tagaacctta tgatgggacg attttttacc gaggtaaaga cttggccaat tataaatcga
     3121 gtgatttttt ccgtcacgaa ttgggctacc tcttccagaa ctttggctta attgaaaacc
     3181 aaagtattga agaaaacctt aagctaggtc tcattggtca aaagttgagt cggtcggaac
     3241 agcggttgag gcagaagcag gctttagaac aggtcggcct ggcttatctt gacctagata
     3301 agcgcatctt tgagttatcg ggcggagaat cgcaacgggt tgccttggca aaaattatct
     3361 taaagaatcc accctttatt ctggcagatg agccaacagc ttcaatagac ccagcaacct
     3421 ctcagttgat tatggagatt ttgctatctc ttcgagatga taataggcta atcattatcg
     3481 caacacataa tccggcaatt tgggagatgg ctgatgaagt gttcacgatg gatcatctga
     3541 aataaaaatc cttgttttta attgcacgat gagttactga aatattatca tgaatcaaga
     3601 attggagtta atttagaatt gtactttatt aatattgagg taactttttc ttgataaagg
     3661 aagaaataat ggagaggaag ttagaatgaa aaaattcgac aattatatta ttgagaagcc
     3721 ttgcgattct aattcagata aactgcaaaa aatcttaata attgaaagtt tggtagatga
     3781 tattttgcaa ttttctctca gaatcaataa tagtgtagga gagattttcc tcctacaacc
     3841 gttttaaaag aaaactatct ttattccatg ttattttgag gaagatattg tgaaagtcaa
     3901 agatgatgat aaagttgagt ggaatttgtt agaatttcaa aaatttagag catttttggc
     3961 ttagtaatct gtgttgaagg ctcaaaacct atggtaaaaa agtagctttg aaaacgtatt
     4021 gcctccaaag atttagttaa ataatgattt aacacaaaaa gaaattattg aagttctgga
     4081 aagatgttgt ttcagtattg agaaaaggtg ggaaaaactt gcgattttca cagagaaagg
     4141 aagaaaaagt atagaaatat agtcaattga aacaagaaca ggataaaaga accttttgtg
     4201 ccatattttt atcctttcgc tttacaattg gattgaacac ctttattgta tcgcgtttgg
     4261 agtttttttg gtataacctt cgacgcgcac ccgcatagcg ggtgtttttt tgtctcgcac
     4321 ctaacggagc gagacaaact aatagtcact taatcaaaaa acgcaccata tcaaaaacta
     4381 aaaagtttga tatcatgcgt catgtcttaa actaattgac tatactttct attcaaatga
     4441 gcttttaacc aattgattga accaatccac tcttaaaacc aaagagcaat ttctcgctta
     4501 gctgactctt ctgaatctga accatgtaca acattttgga taacctcatt ttctccagca
     4561 gcttttgcaa agtcacctcg aatagtgcct ggtaaagctt cttctggacg agttgcaccc
     4621 atcatggtcc gccaagtttc gattactttg ggaccagaaa tgacacccac aagaactgga
     4681 cctgaagtca tgaattcacg aatcggtagg taaaaactct gaccaaccaa gtcctgatag
     4741 tgctggtcaa tcaactcttc tgaaacctgt gaacgaaact ccaatttttc gattgtaaat
     4801 ccacgttgtt cgatgcgctt taacacttca cccactagcc ctctttttac accatctggt
     4861 ttgatgataa agaatgtttg ttccataccc gtctcctttg tcagcttctt tcttttattt
     4921 taccacattt cgtggaaaaa tggagaaagt tttcagaaga gagaatgaga gaacccgagg
     4981 gttctctcat tctctcttat tctactgttt cttccacagt ttcaacggca gtatccacaa
     5041 ctacttctgt tgtttcttca tttccttctt cctctactgg aggattaagg tattcttctt
     5101 cgttgacagc atgtggttca aggttacggt aacgggccat accagtacca gctgggatga
     5161 tcttaccgat gataacattt tctttaagtc caaggagatg gtctttctta ccacggatag
     5221 ctgcgtcagt aaggacacga gttgtttcct ggaaggaagc cgctgacaag aaactgtttg
     5281 tttcaagtga ggctttggta attcccataa ggactgggcg acctgtcgct ggaactccac
     5341 ctgcgataag gacatctttg ttggcatctg taaagtcatt gatatccatg agggtaccca
     5401 tgagaagatc tgtatcacct ggatccatga cacggacttt acggatcatt tgacgaacca
     5461 ttacctcgat gtgtttgtca ccgatttcta ccccttggct acggtaaact ttttgtactt
     5521 caccgagaag gtacgtttca actgacaaga catcacgaac tgcaaggaga cgttttggtt
     5581 ggatagaacc ttctgtcaga gcagcaccac gcgctacttg gcccccaact tcgacacgca
     5641 tacgagctgt aaatggaacg acatattcac cttcgccagt ttcaccctta acaaagactt
     5701 tcttggtacg agttgatgca tcttcttcga tagcagtaac ttgtccttta acctctgtga
     5761 taaccgcttc ccctttagga ttgcgggctt caaagatttc ttggacacga ggaagaccct
     5821 gagtgatatc ggtatttgag gcaaccccac ctgtgtggaa ggtacgcatt gtaagctgtg
     5881 taccaggttc cccgatagat tgggcagcga ttgtaccaac tgcttcacca acttcaaccg
     5941 catcaccagt cgccaagttg ataccgtaac agtgacggca gacaccgtga cgagtgttac
     6001 atgtaaatac agaacggata gtcacttctt ccacaccagc attgacaatt tcacgcgcct
     6061 tgtcttctgt aatcaattca tttggaccaa taatcactgc accagtttct ggatgtttaa
     6121 cagttttctt agtgtaacga ccgttgagac gctcttcgag agactcgatc atctcttttc
     6181 cttctgcgat agaacggatc aagagaccac ggtcagttcc acagtcgtcc tcacggatga
     6241 taacgtcttg ggcaacgtcg accaaacgac gagtcaagta acctgagtcg gctgtcttaa
     6301 gggccgtatc ggtcatacct ttacgagcac cgtgagttga gaagaacatt tccaataccg
     6361 acaaaccttc gcggaagttt gaaaggattg gcaattccat gatacgtcca ttcggagcag
     6421 ccatcagacc acgcataccg gcaagctgtg agaagtttga gatgttacca cgggctccag
     6481 agtccatcat cataacgatt gggttcttag gatcttggtt agcaatcaag cgtttctcaa
     6541 gtttttcacg ggcagcacgc cattcagctg taacagcatt gtaacgctcg tcgtctgtga
     6601 tcataccacg acggaattgt ttggtgattt gttcgacacg tttgtgtgat tcttcaatga
     6661 tttcagcctt gtcatcaacg actgggatat cggcaatacc cactgtcaat cctgcaagag
     6721 ttgagtggtg gtaaccgagg ttcttcatgc ggtcaagtag ggcagaagtt tctgtcgtac
     6781 ggaaacgttt gaagatttca gcgatgatat ttccgaggtt tttcttcttg aatggagggt
     6841 tgagctcaag attgctgata gcttccttga tatctccacc aagtggcaag aagtatttag
     6901 ctggaacacc ttctgtcaag ttggcattgt ttggttcttg caagtatggt agcccctctg
     6961 gcatgatatc gttgaagaga attttaccaa ctgttgtaag caagacctta tgtctttgct
     7021 cttctgtcca aggcttgttg aggctgtctg ttgcgatacc aacacgtgag tggaggtgaa
     7081 cataaccatt gcggtaagcc ataaccgctt cgtcacggtc tttgaagacc attccttcac
     7141 cttcgcgacc agcttcttcc atggtcaagt agtagttacc caaaaccatg tcctgagatg
     7201 gagtaactac cggtttccca tctttcgggt tcaagatgtg ctcagcagct agcatgagga
     7261 tacgagcttc tgcttgtgct tcttctgaaa gtggtacgtg gatggccatt tggtccccgt
     7321 caaagtcagc attgtaggct tcacagacaa gtgggtgcaa gcgaagagcc ttaccatcaa
     7381 tcaagactgg ctcgaaggct tggataccca aacggtgaag ggtcggtgcg cggttcaaaa
     7441 gcactgggtg ttctttaatc acttcttcaa ggatatccca gatacgctca tctccgcgtt
     7501 ccaccaagcg tttagctgct ttgacgtttt gcacgatatc acgggcaacg atttcacgca
     7561 tgacaaatgg tttaaagagt tcaatcgcca tttcacgcgg cacaccacat tggtacatct
     7621 taagagttgg accaacggcg ataacggaac gtcctgagaa gtcaacacgt ttaccgagca
     7681 agttttgacg gaagcgtccc tgtttacctt taagcatgtg gctcaatgat ttcaatggac
     7741 ggctacctgg tcctgtgatt ggacgaccac gacgaccatt gtcaatcaaa gcgtcaactg
     7801 cttcttgaag catacgcttc tcattttgaa cgatgatacc tggtgcattt aactcaagca
     7861 aacgagccaa acggttgtta cggttgataa cacggcggta aaggtcattc aagtcagatg
     7921 aggcaaaacg gccaccatcc aactgcaaca ttggacgaag atctggtggg ataaccggaa
     7981 ggatgttaag aatcatccat tcaggtttgt ttccagactt gtaaaaggca tccaaaacat
     8041 ccaaacgacg gatggctttg acacgttttt gtccagtagc tgttttcaat tcttctttga
     8101 gttcagcaat ttctttttca agatctactt gcttcaaaag gtcttggatg gcttccgcac
     8161 ccatcttggc aacaaatgaa ccataaccat attcacgcaa gcgctctcgg tattcgcgct
     8221 ctgtcatgat agacttgtgc tcaagtggtg tatccttagg atcaatcacc acataagccg
     8281 caaagtagat aacttcctcg agggcacgag ggctcatatc aagggtcaag cccatacggc
     8341 ttggaatccc cttgaagtac cagatgtgag atacaggagc tttcaattcg atatgtccca
     8401 tacgctcacg acgaactttc gtacgcgtta cttcaacccc acagcggtca caaacaattc
     8461 ctctgtaacg aatgcgtttg tacttaccac aagcacattc ccagtctttt gtaggaccaa
     8521 agatcacttc atcaaagagt ccttcacgtt ctggtttcaa ggtacgataa ttgattgttt
     8581 caggtttttt gacttctcca taagaccatg aacggacttt acttggagaa gctagggtga
     8641 tttgcatact tttaaaacga tttacatcaa ccactatttc ttccctttct attctaagtg
     8701 aactgcttat tcttgttcag cagcttcttc tgttgcttcc gcttttgttg ctttctcagc
     8761 ttcttcagct tcaaaggctg ctttagcctc ttgggctgct ttttcgcggg ctttttcaag
     8821 gtcatctacg tggatgacat cttcgtccat tccttcatcc aagtcgcgaa gttccacttc
     8881 ttggtcatct tcgtctagga cacgcatgtc aagaccaaga gattgcaatt ctttgacaag
     8941 aactcggaag gattctggaa cacctggttt tggaattggt ttgccttttg taatagcttc
     9001 ataggctttc aaacgtccgt tgatatcgtc agacttgtaa gtcaagattt cttgaaggac
     9061 atttgacgca ccgtaggctt caagagccca aacctccatc tcaccgaaac gttgtccacc
     9121 aaactgagct ttacctccga gtggttgttg ggtaacagtt gaataaggtc cgactgaacg
     9181 cgcgtgcaat ttatcgtcaa ccatgtggtg gagtttgatc atgtacatga ctccaacaga
     9241 aacacggtta tcaaatggtt cacctgtacg tccatcgtaa aggattgtct tggcatcgct
     9301 atccatacct gcttctttaa cagttgacca aagatcttca gaacttgctc catcaaagac
     9361 tggtgtcgca atgtgaatac caagagtacg agctgccata ccaaggtgaa gctccataac
     9421 ctgaccgata ttcatacgtg atggcacccc aagtgggttc aacatgatgt cgactggagt
     9481 tccgtctgga aggtaaggca tgtcttctac aggaacgata cgagagacaa cccctttgtt
     9541 tccgtgacgt ccggccattt tatctccgac cttaatctta cgtttttgag cgatgtaaac
     9601 acgaaccaac atgttaacac ctgattgcaa ctcatctcca tttacacgtg taaagatctt
     9661 aacatcacga acgacaccat cggcaccgtg tggtacacga agagaagtat cacgcacttc
     9721 acgagacttg tctccaaaga tagcgtgcaa gagacgttct tcagctgaaa gatctttctc
     9781 acccttaggt gttactttac ctacaagaat atcaccttct ttaacctcag caccaatacg
     9841 gataatcccc atttcgtcaa ggtctttgag ggcatcttca ccaacgtttg gaatttcgcg
     9901 agtgatttct tcaggcccaa gctttgtatc gcgcgtttct gattcatatt cttcaaggtg
     9961 aacagatgtg tagacatcgt ccttcaccaa gcgttcgctc atgataacgg catcctcgaa
    10021 gttgtaacct tcccaagtca tgtaggcaac gattgggttt tgtccaagcg ccatttctcc
    10081 attttccata gaaggtccgt cagcgatgaa atcgcctttt tcaacgacat caccaacttt
    10141 tacgagagtg cgttggttgt aagcagtacc tgagtttgaa cgacggaatt tttggatgtg
    10201 gtaaacatcc aatgaaccat cttcacgacg aacttctacc ttgtcagcat ctgcgtaagt
    10261 aactttacca tcatactgag caatcacagc cgcaccagaa tcgtgggctg cttggtattc
    10321 cataccagta ccaacgtaag gtgcctgagg attaatcaat ggcacagcct gacgttgcat
    10381 attggctccc atgagggcac ggttggagtc atcgttttcc aagaaaggaa tacatgctgt
    10441 cgcaacggca actacctgtt ttggtgaaac gtccatgtag tcaacaatat tagctggata
    10501 ctcttggttg accccttggt gacgtcccat gacaatcttc tcagcaaagg ttccatcttc
    10561 attcagacga gagttagcct gagctacagt atattcatct tcttcatcag ctgtcaacca
    10621 aacaatttcg ttcgtgacaa cacctgtttc acggtcaacc ttacggtatg gtgtttgaac
    10681 aaaaccatat ttgttcaagt gtccgtaaga tgacaagtta ttgatcaaac cgatgttagg
    10741 tccttcaggt gtctcgattg gacacatacg accatagtga gtgtagtgca cgtcacgtac
    10801 ttcatatccg gcacggtcac gagtcaaacc accaggtcct aaggctgaca aacggcgttt
    10861 gtgagacaac tcagaaagcg ggttgtgttg gtccatgaac tgtgacaact gtgatgaacc
    10921 aaagaattct ttaaccgcag ctgttacagg acggatattg ataatttgtt gtggtgtcaa
    10981 gacttcattg tcctgaacag acatacgttc acggacatta cgttccatac gagaaagtcc
    11041 caaacgtact tggttggcaa gcaattcacc aaccgcacgg atacgacgat ttccaaggtg
    11101 gtcgatatca tctacacggc caagtccttc agccaagttg aggaagtagc tcatctcagc
    11161 aaggatatct gcaggagtca ccgtacgaac cttgtcatct gggttagcat taccaatgat
    11221 cgttacgacg cgatctggat cagttggagc aacaaccttg aatttttgaa gaacaacagg
    11281 ctcagtcaca acggctgcat cgtttgggat gtagacaatc ttgttcaagt cgccatccaa
    11341 atggctttca atgctttcaa tcacgctacg agtcataatc gtaccagctt ctaccaagat
    11401 ttctccagtt tcagggtcta ccaatggctc tgcaatggtt tggttgagca aacgtgtttt
    11461 aacattgagt tttttattga ttttgtaacg accaactgct gccaagtcat agcgacgtgg
    11521 gtcaaagaag cgagccacaa gcaagctacg tgagctttca gctgtcttag gctcacctgg
    11581 acgaaggcgt tcgtaaattt ctttcaaggc ttcgtctgta cgagagtcca ttggattctt
    11641 gtggatatct ttttcaacag tgttgcgaac caattcgctg tcaccaaaga tatcaaagat
    11701 ttcatcatca cctgagaaac caagagcacg aaccaaggtt gtaaatggaa tcttacgagt
    11761 acggtcgata cgagtgtagg tgatatcttt tgagtcgctt tcaagttcca accaagctcc
    11821 acggttaggg ataacagttg aaccatagcc caccttacca tttttgtcta ctttgtcgtt
    11881 aaagtaaaca cctggtgagc ggaccaactg agaaacgata atacgttcac caccattgat
    11941 gatgaaagta cccatttctg tcatgattgg gaaatcacca aagaaaactt cttgggtctt
    12001 gatttcgcct gtttctttat tgatcaaacg gaaggttaca aaaattggtg ctgagtagct
    12061 agcatcgtgg atacgagctt cttctagcgt gtattttggt tccttgattt catatccaac
    12121 aaattccaac tccattgtgt ctgtgaagtt tgaaattggc aatacatctt caaacacttc
    12181 cttaagaccg tggtctagga aagctttgaa tgagtcagtt tgaatttcaa tcaaatttgg
    12241 taagtcaaga acttctttga ttcttgaaaa actacgacgg gtacgatgtt tcccgtattg
    12301 aacgtcatgt cctgccaaga tgattctcct ttgtaaataa gttccaagcc ttgtcaatca
    12361 ggcttttcta atcgtcatat ggttgtaaac cccttatcac cgtgtcctct tgacgaattt
    12421 tcagaatctt taagcctctg ttacaaatgc tcataatctt gaaaaaaagc acaaaaagag
    12481 cagctaaatc tgactttttc agaagattta actgctgtga gccttgtctg gacaatattt
    12541 cagacaaaac ctacgacaaa tgattaccca tattataccc tatttagcta gatttttcaa
    12601 ggggtttcag taggtttttt gtaaattttt tcccatagaa aacttggcat cacattcgaa
    12661 tcacgctatg gtacaaaaaa ctgaaaaaac tattgactga aaatcatttt caaggtataa
    12721 taataaacgt taaggcggta tagccaagtg gtaaggcacg gctctgcaaa agcttgatcg
    12781 tcggttcaaa tccgtctacc gccttctata acttgattta tcaggtttca aatgaacaga
    12841 aagcccaatt tgaagggctt ttcttatttt ccctcgaata aatacgtata actttaaaaa
    12901 cttttggagc gagtttgtgg cagagttctt tccatggcat aattcccttt tgaaatcaat
    12961 tgaaaaaagc ttcgtcacgc ctggtgacaa aagcctctag tttattctgt ttcttcttct
    13021 atttcgactt ctgtgatttg gacttttacc ttggtaacac gtccattctt cactttatca
    13081 ttggttaaga taaactgttt gttttggctg accaattcat agctgagttt ctcagtcgtt
    13141 ggaatggttc ccactcctgt caaataataa ccagcgatgg tatcaacgtc atcactttcc
    13201 agttcaacat caaagtaatt attgaaatca ttgagggtca tagttccttg aacaatgtag
    13261 gtatcctcgc caatctgatg aacatctatt gctgctttgt ccgtttcatc atcaatttca
    13321 ccaacaattt cctctaagag gtcttccaaa gtcaccaaac cagccatacc accgtattca
    13381 tccagcaaga ttgccatttg tctttgggta tttcgcaatt cttttagcaa gtcatccaca
    13441 aaaatagttt caggtacaaa aagtggatct tgtaaaattc tcttccaaac aatattgtca
    13501 aaaccgtcca caaagcctgc cttaaggaga ctcttggtgt gaatgattcc aattacattg
    13561 tccttatccc catcataaac cgggatacga gaataatttt gttttaaaat actttggata
    13621 atggcttgac tatcatcctg aatatccacc ataaaggcat ccgttcgagg aaccataacc
    13681 tctcgtgcca tcagttcatc gagcgaaaag acaccttgta gcatctcaat ctcatcagca
    13741 tccaatgttt cttcactatt tgtcagcata taggcaattt catcacgggt catcttttca
    13801 tccgcatcat cgaatgacat aggagtcaaa tggctcaaga aattggtcga agcagctaaa
    13861 agccaaacaa aaggactgac tagttttccg atcccaatga taaccggcgc tgtacgaatt
    13921 gccaaggcat cctttagatt aagagcgatt ctcttaggat ataattcccc aaaaacgatg
    13981 gaaatatagg tcaaaaatgc caaggataga aaagttgcca cggcttgtgc tgtttcgcca
    14041 ttcccaagcc aagaggcaat cacacgtcct agagtatcag ttaaactcgc ccctgataag
    14101 attgtaatca gggtgattcc tacctgaatg gttgataaaa agtggttagg attttctagt
    14161 accttcagca ggcggatgta gcgtctgtct ccttcttccg ccttttgttc aactcgggca
    14221 cgattaagag aaaccatggc catttctgtg gctgagaaaa aggcatttaa taccgtcaag
    14281 ataaacaata aaacaaattg tagcaacaaa ttctgactac ttgggtcttc catagttctt
    14341 ctcctaaaat agtatcttat cctttattat atcacaaaat ttaatccaat acatattatt
    14401 tttttcttag cgtaacttcc atctctctcc caaaactgga agttagtcta agacggattt
    14461 ccactgattt cacgcgtttt tctcatacta aaagctaggt tgtgggaaac tggaagttag
    14521 ttttagaagt tactattttt tcttagtacc tgttctagcc ctgcttaaac tagtaaaaag
    14581 aactcctaca tccataagtg acataggagt ttaggcttat cttttactga gttacagtta
    14641 cttctccagt tcggtaatcc agttgaagtc ccttttgaac attgggaact agaacattga
    14701 attccaattc tccatccgaa gacttggctt caatctgtga agctgaggga actaaatcct
    14761 cgtttgaagc gtagtaaagg gttacacggt aacggacacg cttgttttgg tccaaggctt
    14821 tacgcacctt gctttcatag tagttttgac cagtcgaata ctcggcttgt gcctgatttg
    14881 cccaggctgt ctgaacagca atgtttttag gattgcttgt tgaggcatca aaaccatcca
    14941 aaccaccgat taaggcatag cctaacaaat gacctctatc gactgcatgg gtataagagc
    15001 cctttagatt cttgacctga tgccaacctg gaggagtcca agaagttgaa ccattcccag
    15061 tttctttacg attcttgtac tgacgagtgg ccttagacaa gagggcatta gctacggttg
    15121 gaacagtttc cttgcccact gtctttgttt tattgtcagc gtagggctta cttgaaacct
    15181 tggcatctag atttgtttta ttaccattga cgataaaagc acctgagcca ttccactcca
    15241 gactcccctt tatttgactc ttgactgcgt ctgttaagac actctctgcc aattcttgac
    15301 taggagcttc aggcgcttgt tttttctgac tcatttttgt cttagcagcc tgatttccta
    15361 gttcattctg cttaatataa tagctccctg cagacaaaag caataacact agcagtccaa
    15421 tcagtgtctg tcttgttttt ttgttcatat ttctccttat ctttataaaa aggctggtct
    15481 cccagcctaa tttcctgtaa atttatgaat caattcctgc cattttgccg gagacaggat
    15541 agcccacgga tcttgaccct tgcccaagat tccatagcct accattaaac cgattcctaa
    15601 ggctagaagg cctaaaatca gtaccagaat gactaaaagt aaacgcttga ttacgtaacc
    15661 tgatttctta ttcatcttca tcatttgcct caatccgctg aatcttagca cctagctgcg
    15721 ccaacttctc atggaaaccg tagtaacctc tatccaagtg aaccaattta ccgaccacag
    15781 tttctccctg tgctaccaaa cctgtcaaaa tcaaggccgc actggcacga aggtcagttg
    15841 aaagaacttc tgctccctgc aaaggctgtc caccaacaat acgagctgta tcacggataa
    15901 tctcagaatg caagcccatg cggcgcatct cttctaggtg ttggaaacga ttttcaaaaa
    15961 ctgtctccac catggttgat tcgccttttg caactgtcat caaggctgta aattgagcct
    16021 gcatatctgt tggaaatcct gggtggggca aggttttcac atgaacagct tttagatttt
    16081 ctagttgaga acgaacacga attccttcgt cttcttcaat tacttcaaca cccatttcaa
    16141 gtaacttggc aatcaaggga cggttgtgct cccagacagc gtctcgaatc aagacatcac
    16201 caccagtcat ggcagcagct accataaagg ttcctgcttc gatacggtct tggactacat
    16261 tgtgagtcgt accatgaagt ttctcaacac cagtaatggt tatagtctct gtaccagcac
    16321 ctttgacctt ggctcccatt tcattaagga gaatggctaa gtcaacaatc tcaggctcac
    16381 gcgcagcatt ctcaatcact gtcaccccat cagccagagt cgctgccatc atcaagttct
    16441 gcgttgcacc aacacttgga aagtccatat agatatgagc gccatgcaag cgttctgcct
    16501 tggcttcgat gtaaccagct gtctgactaa tcttaacccc catagcttcc agacctttca
    16561 aatgaagatc aataggacga ctaccaatcg tacaaccacc tggcatggat accttggcat
    16621 gacccacacg ggcaaggatt ggccctaata caacgatgga ggcgcgcatc ttgctgacat
    16681 acttgtaagg ggcttcctca gtgatgtcgc cagtagcatc caccttgaca agatgagctt
    16741 cctcatcaaa gtcaaccttg gcattcaaac caccaactac ctgattcata ataaagacat
    16801 ccgacaaaat cggaacattc tgcaagacgg tctttccttc acttgctaga atagtcgctg
    16861 ccaacaaggg taagactgca ttttttgctc cctcgatcgt cacgcttcct accagacgat
    16921 tatcgccacc ttgaactaca attttatcca tactcgtttc ctttactctt gattttataa
    16981 taatcctcta agcgcttctt gccacaaatg atacaagcta ataaagaaag aactcaggat
    17041 gtagcctatg acaatgctaa aaaaggctac taataaacga acttttcttg tattatcatc
    17101 tgtcactttt aaaacctttt cccatctaac aagatccttt aaaaggtaaa aactcacata
    17161 aataaagagc atatgactac ttagagtgaa taataattga accatttgac tattatacca
    17221 tcttctctgt ttgtgcgcta gtttggaaac ttcactcaaa aattagggat tgtttttcaa
    17281 gtaatcaatc gcatcttgta gggttttaac gggcacgatt ttcatatccg tcttgattgt
    17341 tttagccgct tctagggctg tttggtaatt gtttttcgcg tccggatgcg ccttttgttc
    17401 ttcttcgcta acagggttat caggagcaaa gaaaatagca gcacctgccc tagccgaagc
    17461 tacaaccttc ttatcaatac ctccaatgtc tcccacatta ccatcgcggt caatggtacc
    17521 tgtaccggca acaatacgac cattacgaag atctgggtga gctatttgag tatagatagc
    17581 tagactaaac atgagaccag cacttggacc gccaatacca gctgttgaaa agctaattgg
    17641 gacattgctg attacctctg tacggtcaat caagccgatt ccaattccat ttttgccatt
    17701 ttccaaggtg ataatctttc cttctgcaga cttggtttgc ccatcctctt cataggtgac
    17761 cttgacggaa tcccctaatt tttgagaact gacgtaatca atcaagtctt tggaactatc
    17821 aaaggtctga tcattgactg ctgtgactgt atcagagata ttgagaatcc ctttaaaggt
    17881 tgaattatcc gtcacattca aaacataaac tccaaagtac ttgagttcga tatccttacc
    17941 agctgttttt agtccttgat acttggccat attttgcgat gtttgcatgt agaattgatt
    18001 gattcgcata aattcaacat cggaagaacc acctgtagtc tcctgagcac tacgaatatc
    18061 tgtaaaaggt gtcaaccaag cataaatcat atgagctaaa gtggcatgtt gaacaccaac
    18121 cgtaacgaat tgataagcac cagcttcctt atcttctgtg tcatttactt taaggacttg
    18181 gcgaatatct tccgaaccac ctggaacctc tatataataa ggcaagggca ctacaaatgc
    18241 caagaaagtc acaatcaagg ccgcaatgac gtataagggc catctaatct tttttttcat
    18301 ttcttatttc ctccaaaata ctctcgggaa catggcaggc aatatcctga ccaaacttca
    18361 aaagctctcg aacacctgat gaactgatat agagatgttc aggccggcta tgtaaataaa
    18421 tagtctctat atcagaagac agctgatgat tgtagtaatc aaaactagct tcatattgca
    18481 aatccgccgc attcctcaac ccccgtacta ggaaagtagc ctccagtctt tttgcaacat
    18541 cgaccaccaa tttatcatga gaagccacga cttcaacatt ttccaaatgt cccaaagcct
    18601 tttctagccc ccgtttacga ttttcaagag ggagaaatcc ttgtttgtgg ggattaaaaa
    18661 aaatacccac ataaagctta tcaaaaagtc tgctcgcccg ttcaatcata tccagatgcc
    18721 catttgtcat cggatcaaat gagcctgtga ataagccaat cttatctgac atagactgtc
    18781 accttactaa ttccataaat cttttccttc cagataccca gacaggcaat ttcttctgga
    18841 agttcaacgg ctttatccgt ctcgcacaca accataacat cttcagaaaa aagctctctc
    18901 tcagccattt tttcaatatc tgctacgatt tgttccttgg catagggagg gtctaagaaa
    18961 acgaggtcaa attccccaga tacctgttcc aatgcccttt ctgcatccat cttgaggagt
    19021 tgaaattttc caacttcctt ggtcatctgg atattttcag ccacgatggt ctgagcctta
    19081 cggtctcgct ccaccaaaac agcactggac atgccacgcg atactgcttc gatagataaa
    19141 ccaccactac ctgcataaag gtccaagact cgtcccactt caaagtaggg accaatcatg
    19201 ttaaaaatgg ctcccctaac cttatccgaa gtaggtcttg tcgtcttgcc ttctagtgtc
    19261 ttgaggggac gtcccccata gattcctgat acgattttca tactgtttat tataccaaat
    19321 tatagacaaa aagagaaaga aaaccgaacc ttgcggttcg attctctaca aaatattttc
    19381 gtaagtatcg cggacttctt gaggccaaac acttgtttgc acttctccga tgtgtctctt
    19441 gcgaagtagg aacatggcca tacgagattg tccaattcct ccaccgattg tcaatgggaa
    19501 taggccattc aacaaagact tgtgccattc caattccaag cggtcttcat cacctgtaat
    19561 ttccacctga cgtctaagag tttcttcatc tacacgaatt cccatagaag acaactcaaa
    19621 ggctccacct aaagactcat tccagacaag aatatcacca tttagaccct tgtagccatt
    19681 ctcagactcg cttgtccagt catcatagtc tggtgcacgt ccatcgtgcg gtttaccatc
    19741 tggcaactcg ccaccgatac caatcaaaaa gacggctcca aattctttac aaatcgcatt
    19801 ttcacgttct ttcggtgtca agtctgggta gcgttctacc aattcttctg tatggataaa
    19861 ggtgatttgt tttggcaaga tagactcgat gtcatagcgg gcttcaacag ctagctcagt
    19921 caggcgaata gccttataaa tcttctcaac tgtttctttt agataaacga tgttacgctt
    19981 accatttggg ataaccttct cccagtccca ctggtcaaca taaacagagt gggttgcatc
    20041 caaggaatcc tcatctggac gaagggcttt catgtggaca aagagaccct ctccttcacc
    20101 aaagccaaaa cgagccaagg tgtggcgttt ccatttagca agtgagtgca ccacttcata
    20161 agtagcatca gggatttgga gaaccttgac cgatactgga ttttccacac cagacaggtt
    20221 gtcctgcatt ccgtcaccaa ccttactcaa gataggacct tgaacttcga caacttctag
    20281 cttatctttc aaatactggg taaaagtgtt tttgacaaag gaaatttctt cttgttgatg
    20341 gataaaactt ttcttcatag acaactcctc aaaaatttaa taaacgtatt atacacctat
    20401 ttccaagaaa agaaaagaga aaatttaaaa aaaatcagtg tcactcaaaa cactgatttc
    20461 atagaccttt tagtcattgc gaccaaagat acgtagaata cttaggaaaa gattgataaa
    20521 atcaagatag atgctgagag ccattgacac cacccagcct gtcgctacac gaccttgtga
    20581 ttgttcatag gcgaggcgaa tcttttggtt atcccaagca atcaaccctg aaaaaaccaa
    20641 aaccatggct acgctaatca tataatcaaa gaagccacta gccaagaaaa tattgactac
    20701 catagcaatc agtaaaccaa tgagagctgc catcattgct cgaccaatcc cactcaagtc
    20761 tttcttggta aagataccaa ctgccgccat gacaaagaag agaagggcgc tcgatacaaa
    20821 ggcagataaa actgtacccg gagtatagaa agccaccaca aaaccaaggg taaagccgtt
    20881 taaaacggag taaagtaaaa atactggaag agcagctgga ctatttcttg aagccatgct
    20941 actggcaacg aaaaccaaag ccaactccgc aaaagtagca atggtcaacc agagacgccc
    21001 ctgcatcaaa aagtaaacca actgagactg aaagaccgtc aacataaggc ctgataccaa
    21061 agcagatagt ccgattccca gaccaacaaa ggcataaacc ttagcgtaaa attgattgag
    21121 acctgcacgg tcatgtataa tagtgtgatt catccatttc tccttttctg tgaacatctt
    21181 tccttgatta taacaaaaaa agttaaaatt agcttaaatg gaagattaaa ataccggctg
    21241 caaccgcaac attcaaactc tcggcctgcc ccttcatgct aatatggacc aactggtctg
    21301 cactttcagc cataagggga ctaattcctt gaccctcatt tcccataact agtacaaaat
    21361 tttctataga aggcagttct ctgtaatcaa cagaatcttt agatagggtt gttgctagca
    21421 ctgggatagc taccttttta gtttcgtcaa gaagcgcttg actagtcatc cggtaaatgg
    21481 gcagatggaa atgactacct tgcatggaac gtagggtttt gagactgtag atgtctgccg
    21541 acttatctga aacaatcact cctgtaaaac ctgctgcatc cgcagttcga atgatagttc
    21601 ctacattacc aggatcttgc acatcttcca aaaacaagaa cttgccctga ctcaaatcag
    21661 cttgtcctac ttcttctttt tgaaccacgg caacaattcc ctgtggagtc tgagaatccg
    21721 ccaaatctag caaaatatcc tctgaaatcc agacagtttg cggaaaagca gctaactgat
    21781 ttcgataatt ttctagggca aagattttct caatcgtcac tccagcttgg acagcttctt
    21841 caaacaagtg ccagccttca atcaaatagg cagacttgcg gtattttttt tggtgtaatt
    21901 tcttagcatt ttttaccaca gaattggctt ttgaggttat aatagtcata gaaatattat
    21961 aacacaatca aaggggtttg gtatgcaaaa ggttagaatg attgcccaag gtagggtgca
    22021 gggagtcggc tttcgttggg gtgtttatag cctggccctt gaaatcggtg gcatcacagg
    22081 tcgggtatgg aataacgacg atggcacagt ggaaatctta gcccaagcag actcatcagc
    22141 tattatggca aaatttatcc aagaaatccg aaaaggaccg acaccttttt caaaagtcag
    22201 ttacttagat gtcaaactga gcaactttcc tccctaccct gactttaaaa tcgcaaatta
    22261 ggtctccaga actattgtat attttgtaaa aaaacagtag aatagaaagg tataattttt
    22321 aaagaaggaa taaaaacagt gaaatctatt aaacgttttg cactctcggc tatgggagtg
    22381 gctatgttgc ttgtcttgac tggctgtgtc aatgtcgata aaaccacagg tcagccaaca
    22441 ggatttattt ggaatacgat cggagcgcct atggctgaag ccatcaagta cttcgctact
    22501 gataaaggtc taggctttgg tgtcgctatc attatcgtaa ccattatcgt acgcttgatt
    22561 atcttaccac ttggtatcta ccaatcatgg aaggcaacgc ttcactctga aaagatgaac
    22621 gccctcaagc acgtccttga gccacaccaa acgcgtctca aagaagcgac tactcaagaa
    22681 gaaaaactcg aagcccaaca agccctcttt gccgctcaaa aagagcacgg tatcagcatg
    22741 tttggcggtg taggatgttt ccctatcctc cttcaaatgc ctttcttctc tgctatctac
    22801 tttgctgccc aacatactga aggggttgct caagcaagct acctaggcat tcctctaggt
    22861 tctccaagta tgattttggt tgcctgtgct ggtgtccttt actatcttca atcgctcctt
    22921 tcacttcacg gagtagaaga tgaaatgcaa agagaacaaa tcaagaaaat gatttacatg
    22981 agcccactca tgatcgtcgt cttctccctc ttctcaccag ctagtgtcac actttactgg
    23041 gttgtcggtg gtttcatgat gattctccag caatttatcg tcaactatat cgttcgtcca
    23101 aaacttcgca aaaaagtccg tgaagaactg gcaaagaacc catcaaaagc aagtgctttc
    23161 tcaacaccaa gtggacgaaa agacgttacc cctgaacaac caactgctat cacaagcaag
    23221 aaaaaacaca aaaatcgcaa cgctggaaaa caacgttcga gataagaaga aaaaggctta
    23281 gctgatttgc taagtctttt tgcaagtcaa aagcccgatg tttccatcag gcttcttttc
    23341 tatccatgta cacgtttcat ataatcttga taactttcgg tatccatgag ttgtttagcg
    23401 ttcttgacgc gatctgctgt tggtggtttg actccttcga gggaatatgg aattccaagt
    23461 tcacgccact tgaactcacc catggtgtga taaggtagaa tttcaaactt atcaacattt
    23521 ttgagggtct tgacgaactt accaagttca atcaagtcat catctctgtc tgtcaatcct
    23581 ggaactagca cgtggcgaat ccagacaggt tttccaatat ctgatagata ctgggcacaa
    23641 gccaagatat ttttattggt ttggctagtg acaatcttgt gctgttcttc gttgatttcc
    23701 ttgatatcca aaagaaccaa gtcagtgaca gccatgagtt tgtcaaactt ctcaaggtaa
    23761 cgcggtttat tacggaaagg aagagcacag gtgtccaagg tacagtggat tccttgttcc
    23821 ttagccttgg tgaagagagc aatcaggaaa tcaatctgca agagagcttc tcctccactg
    23881 actgtaatcc cacccttatt tccccagaaa ccacggtagc gcaaggcctc tgtcaagaca
    23941 tcatctaccg tccgttcacg tgacttattg gactccatag cccaagtgtc tgggttgtgg
    24001 caatactggc aacgcatgtg acagccctgc aaaaagacaa taaagcgaat accaggccca
    24061 tctactgacc caaagctttc tgtcgaatgc accattcctg tcacttgtcc ataatcaatt
    24121 gtttcttcag acatatcgtt accttccttg aaaacgtttt atagttttat tatatcacga
    24181 cttgaaggaa aaacaagatt tttgcctatt ttttagaaag caatctgttt ttcaatttca
    24241 ttttcaatct attatcagat tattcttcaa aatcaaagcc atataaggtt gcaaaatagt
    24301 cctcagggcg ctctgcacgg cggatttgac gggctttacc ttcttcggta tagaggattt
    24361 ccgcagaacg taatttggca ttatactggt agcccattga aaatccgtgg gcacctgtat
    24421 catgaatgac cagcaaatca ccgatttctg tatgaggcag ttcgcgattc actgcaaatt
    24481 tatcattgtt ttcacagagt gaaccaacca catctaccgc ttcagctggt ccatctggat
    24541 gggtcacgtt gctaatatga tggtaagctc cgtacatagc tggacgcatg aggttgactg
    24601 ctgaggcatc cacacctaga taggtacggt aggtttcctt cttatgagtg actcttgtga
    24661 ctagagcacc gtgaggtgcc agcataaaac gacccaattc ggtgaaaatc ttgacctgac
    24721 caagacctgc tgacgtaaga acttcttcat acaccttacg aactccctca ccaatcaagg
    24781 cgatatcgtt cggctcctgg tctggacgat aattaacacc aataccgcca gaaagattga
    24841 taaagtctag cgaaatgccc aacttttcct tgatttcaac agccagttca aagagctgac
    24901 gagccaactc tggataatag agatgggtca cggtattgga cgctaggaag gagtgaatcc
    24961 caaaagtctt ggctcctttt tccttcaaga tagcaaaggc ttcaaagagc tggtccttgg
    25021 tcatgccaaa cttagcctcc ccaggattgt ccataatgtc tgtccccagt tcaaaaacgc
    25081 ctccaggatt ataacgacaa gagatgattt ctggaatacc tgctgctctc tccagatgtt
    25141 caatatcttc aaaggcatcc aagttaatgg tcgcacccaa ttcacgtgca taggcgtatt
    25201 ccttgtctgg cgtgttgttg gaagagaaca taatctcaga acccagaaag tccagtttat
    25261 ggctcatcaa aagctctaca taactagagc agtccacacc acaaccttct tcttggagaa
    25321 ttttcaaaat agctggagtt ggagtagcct taactgcaaa atattcctta aagcccttgt
    25381 tccacgaaaa agcttggttg acggctcttg ccttctcacg aatccccttc tcatcataca
    25441 agtgaaaggg agtcgggaac tcggcaacaa tcgcttctaa ctcttctcta ttgataaatg
    25501 gtgttttcat aagcttcctt ctattctgtt tgcaaagaaa ggctgggaca atcgttccaa
    25561 cctttagttc tatctcttat ttatcttcgt caaagatatt gccaacctca acatcgatgg
    25621 cttggttctt gacatcaata ttggttacct tcaagagtga cttgtagtca aactgctttt
    25681 cacgttcttc ttgggcattg tccgcaaaga ccgctacacc tgccagttct gagtcgaact
    25741 cgcgcaagag actaatcata ccattgaccg ttccgccacc tttcaagaag tcatccacaa
    25801 tcaagacacg gctgcctgcc ttaagactac gttttgaaag gaacattttc tcgatacggt
    25861 caccacttga acctgaaaca tagttgacgc taacagttga accttcggta attttcaggt
    25921 cacggcgcac aatgacaaaa gagacattga ggacattggc aactgcattt gcaagtggca
    25981 cacccttagt tgctacggtc ataaccgcgt caattttttg gtccataaag cttttggcaa
    26041 taatacgacc aatatttttc aagatggctg gtgtgctaag caaatcagac agatagatat
    26101 aaccacctgg caagatacgg tcactttctg acaacttggt acgcaagtct tcaaccattt
    26161 ccttagcatc ctgactcgaa atagacggtg tgaagatgac acctccgcca gccccagtca
    26221 ctgtctggat atgaccgatt tcaatttcct caaaggcgcg tttgataatg acgatatctt
    26281 ctgagatgga tgatttagca gactcatact tttcagcaaa agtattgaga ctagttagtt
    26341 tataaggatt attaatcaaa tagttggaaa tgacaaccat ccgatcactt cttcttaatt
    26401 tcatttctat ttcctattcc atttttattc aaaaaatcaa aaagcaaact agaaagctgg
    26461 tcgctggtgg ttcaaaacac tgttttgaga ttgtcaatag aactgacaaa ccctgtaata
    26521 tacctgcata tatacatacg acaaggcgat actaccctag tttgaagaga ttttcgaaga
    26581 gtattcattt ttgtctttta cttattatac catattcaca taaaaaaacg aacattctta
    26641 tcctaaaaaa tgctcatttt tcttaaatta ttaatctaaa tctggtttat agaaggaacg
    26701 attatccata gcgaagattt tattggacat ctctccttta tccaccaaag ccagagctgt
    26761 tgacatcatc atcatgcttg catccagatt gtcaatcata tggataatct ctgcctccat
    26821 aatacgtgga cggactggac ttccatattc aagcaagccg tggtgactga ggatgacatg
    26881 acgaagcaaa acgacttctt ccttggtatc atcgatgccg agttccataa ctgtcttggt
    26941 aatttcgcta tcaatcagag cgatatgtcc aagaagattg cctctgaccg tgtactcggt
    27001 ctggtctggt cccgtcaact cgatgacctt agccaagtcg tgcaacataa tccccgcata
    27061 gagcagactc ttattgagct gaggataaac ttcgctaata gcgtctgcca aacgtaccat
    27121 ggtcgccgta tgataagcca accccgtttc aaaggcatga tggttggtct tggcagctgg
    27181 ataggagtag aattccttat catacttggt gtagagattt cggacaatcc gttgccagac
    27241 aggattttca attttgaaaa tcatttgcga catgtagtca cgaatttcct tgacatcaac
    27301 tggtgacttg accttgaaat cggctgggtc attgggttca ccagcttgag gcaggcggag
    27361 agtaatttga ttgacttgag gggtattgtt ataaacttct cggcgtcctt tcatgtggac
    27421 aaccttacct gcggtaaagg cctcaatgtt atgaggttgg gcatcccaga gcttcccatc
    27481 aatctcgcca ctatcatctt ggaaggtaaa ggctaggtag tttttcccag ctcgagtttg
    27541 cctcaggtca gctgatttga ttaggtaaaa gccttcaaat agctcatctt ttttcatgtg
    27601 actaatcttc atattcttcc tcattttctt gaaaatggag tagatcaagc gcaggctcgc
    27661 cttctgacaa ctcaatgtga cggagcgtcc gctcgatagc tatggtacga cggtttaata
    27721 attcatcaat attgccagag gcatgttgga gatgtttttg tgccttgacc agaataccac
    27781 caaacttgcc aaactcggtc ttgacactgg caagagtctt gctgatatgg tcggcactct
    27841 tttggatatt aagggtcttg aaaccaactg atagggagtt aagaagggct gatagggtac
    27901 ttggtcctgc aacgataatc tgttcttccc gtctcaaatc atcaaagaag accggattgc
    27961 gcacaatctc tgaataaaga ccttctgtcg gaacaaacaa gattccaaaa ttggttgtcc
    28021 gaggcggtgc tatgtacttg ctcttgatat ccttggcaaa gcgcttgacg cttgctagga
    28081 gtgacttacg acagcgttca atctcatcct tgtcacctgt ctcataggct tcttccaagc
    28141 ggtaataatc tgccagtgga aacttagagt caattggcag atagacgtat tcttggtcgc
    28201 cttgtccggg taacttgatg gcatactcca ctcgttcact agagttttca accgttgcgt
    28261 attctcgttc gtactgggca ggtgtcatga tgtcttcaat aatttgcccc agttgcaatt
    28321 ctcccagaat acctcgcgtc ttggttccag agagaacctt gttaagagct ccgacatcac
    28381 gggcaactgt ctgcatttct ccaaggccac gattgacaga ctccagttgt ttagaaactg
    28441 tttcaaagga agcctgtaag cgtgtctgca aggtcttttc tagtttttcc tcgaccgtct
    28501 ggcgcatttg ttccaaacgt tgctcatttg attcctgcaa ggcttggaga cgttggtctg
    28561 tcttgtctct agtttggagg agattatctg tcatttcttg acggacttgg gtcaggcctt
    28621 ggtgcaattc aatccgcact tcttgcaaac ggtcgctgac agccacttcc aaatcttttt
    28681 ggtctaactg gctggcttgt ctggcttggt caaagcggta atccaactgg tctgacaaat
    28741 gatctgcctg atcctccaag ctcttactta agtgtttctc ctgcctatcc tgcctttgcc
    28801 aaatcagaaa gagaccagct aggttggcaa ttaataatag aataagtaaa ctttccatcc
    28861 tacctcctgt ccttgctatg cagtacgacc acataaccat ctgggcaagt taccgacact
    28921 tccctatcta tatattcgtt agaagcgtac acttttttaa agaaaaaatt ctcctctgtc
    28981 aactcatact tagctccaag aatagtcagc tggctatccc gaactggcat aaaggctaga
    29041 tagtcgtagt ctgaacgagg ttctagctga ctggtccctt ctgaacaata ggcaatcaaa
    29101 ttttgcccat cctcaatttc tatttgacgc atatagggtg ccaacttagg attgctaggt
    29161 agaaagacat tggccaacat atggtcaata cggccaccca aggcaccgaa aatagtgacc
    29221 tgagcctgag gattttgttc aaagatggtt aagagagcca attccagatc tgtatcatcc
    29281 ttttctggtc gggcttggac aaaatactgg gcacgttttt gaatcacctg tcgctcttct
    29341 tccgtcacag aatcaaaatc tccgactgct agagcaagag gtaagtcttc ttccaagacc
    29401 cagagcgagc ctcgatccac cccaacaaaa gcatcaaaat ctgtccgata atgaccgcgg
    29461 tttccgcctg caaaaactgc aaccctagtc cagttgtttt ctgagagttt gtactcgctc
    29521 attgacttct cccttaaaga cataggaacc tgctacaaaa acagtcgcac cagcttcttt
    29581 ggcttgagca atagtttggt catcaatccc accatccact tcgatttcaa agttcaaacc
    29641 tttttcctca cgaagagcaa ccaactcacg aaccttatcc atggtttctg gcagaaaggc
    29701 ttgcccacca aaacctggat taaccgtcat gactaaaact tggtcaacta gatgaaggac
    29761 gtgcttgatg gcttcaactg gtgtgccagg attgataacg actgaaggct taactccgag
    29821 tgaacgaatt ttttggaggg cgccatgaat atgaggtgtt gcttctacat ggatactgat
    29881 gatgtctgca cctgcacgcg caaaatcttc cagatgatgc tcagggtttg acaccatcaa
    29941 gtggcaatcg aaaaccatct tactatgagg acgaaggctc tcgaccacac ctgcaccaaa
    30001 actgatttgc ggtacaaaat gactgtccat gatatcgata tgggcatatt ctgccccagt
    30061 tgcttctaga cgtttgattt cacgttcaaa gttggcataa tctgctgcca gaattgacgg
    30121 agcaatcttg tattgagaca taggtttctc cttattttgg aatttttttg ctgacttttt
    30181 tataggtttc tctacgattt tcaatttcac taaggaattg caggtaattg tcaaaacgga
    30241 aggttgcaat aacaccctct tcaacagctg gtttgacggc acaagacggc tcatgggtat
    30301 gggtacaagt acggaactta caatctcggc taacagtagc aatctctggg aaagcctgat
    30361 tgaggtcttc agcccttgat acttcatagt ccaaggatga aaatcctggt gtatctgcga
    30421 ttttaccccc gttgagattg taaaaactaa cagctcgagt ggtatggcga ccgcgaccta
    30481 gactgtctga aatttctccc gtttcaagat tgaggtctgg tgcgatttta ttgagaagag
    30541 ttgacttccc aacacctgtc tgccccataa agaccgtaac cttgcctgtt aacaaagaca
    30601 ggagttcctc tttactggtc acaaagtcat agccgatgtc accataggtc tgctggtaaa
    30661 aatccagttc ccccctatct tccaacaaat ccattttgga aatatagaca atgggatgga
    30721 tgcccttgtg ctccaaaaga accaagaaac gatccagcaa attgctgtta aaatcaggtt
    30781 ccttgacgga catgattact acagcttgat cgatattgac aataggcgga cgaaccagac
    30841 tgtttttccg ttcgtgaatt ttgaggatat agccttctga attttcctcg gcagagaaat
    30901 ctacccagtc cccaacataa ggggtatggc cttttttacg gaaattccca cgcgcgcgtg
    30961 tttgataaac ctggccatca ctctccacat agtagaaacc tgccaaggct ttaatgattt
    31021 gtccctgcat cttagagtcc ttgcccttta agtgcgtctg ctagaccggc aaattctgcc
    31081 aagctgagag cttccccacg cacacttggt gacaagcctg cctggtccaa agccttggtc
    31141 agcttgtcct tgacctcttc agtcttacca aagtaacctg tcaagttatt ccacaaggtc
    31201 ttgcggcgat gggtaaaact agccttggaa accttaaaga aaaagttctc atcttctact
    31261 gctacggctg gctctggacg acgcaccatt ttcaagatgg ctgaatccac atttggcgct
    31321 ggcacaaaga ccgtacgagg cacgataaag gcaaccttgg ctgtcatgta atactgcacg
    31381 gcgatagaca agctaccgta agccttggtg ttaggctggg ctgaaatgcg gtccgctact
    31441 tctttctgca tcatgaccac aaactcacaa aaaggaatgc cactctcaat caagtgcatg
    31501 agaataggcg tcgtgatgta gtaaggcaaa ttagccacta ccttgattgg caggttagga
    31561 tttttaaaat tttggatatg ttgcgccaaa tcaaccttga gaatatcttc gttaactaca
    31621 gtcacattat caaaatcacg cagggtatct accaaaattg gcaccaaacg gtggtcaatc
    31681 tcaaaagcca tgacttgggc tgcacgctca gccagaaact ccgtcaaggc accaatacct
    31741 ggcccgattt cgatgacatt gacctggtca tcaatttcag ccgtatccac aattttttga
    31801 aggatattgg tatccgtcag gaaattttgc ccgaaggact ttttaaaggt aaaaccgtga
    31861 cgctccagca ctgccttggt cacgctataa tctgcaattc tcatctattc tcttttctat
    31921 tatctgttac tactattgta acacattccc cttgtatttt gggaatgaaa atcataccaa
    31981 aaaactatct ttcctcatga agaaagatag tcttggaaat ctttaaaagt ggccttaaca
    32041 gttctctcga aacatgcgtt caaaaaaatc agcttcttga taacttcgat tttcaggtag
    32101 ggtcaaatcc acatcctcct ccacatcatc cactgtcagt tcaaccgtct gaggtaaatt
    32161 tttcaaacga taatgctttt gcctttgccc ttcaatacta gcaaaatcaa gcaaatagcc
    32221 atgcactcgg atttgaacca aaaggagatg attggtcata ttttccatga tgaggatatc
    32281 ttctaaacca cctctggcac taggataatc agataccctg cgaatacgga ttttttcaca
    32341 catgagcaag tttcctcata tcaatgattt catcagcctt attccagact aggggattat
    32401 gggtcgcaat gatgataatt cgattttcat ctttcaaatc caccaagaga ttcataacct
    32461 cctctgaatt ttcaggatca agagctgctg ttggttcatc tgccaaaatc aagggtggat
    32521 ttttcaaaat agtcttagca agggcaactc gttgggcctc tcccccagat aaagtataaa
    32581 ttttttgttc taaatccaaa taccctagat taactttttc taaagccccc acttgccttt
    32641 ccaaacgttc tacttttgag attttctgac caacaaaacc caaatccaaa ttttctttga
    32701 ttgattggtt ttctaagagg ccaaaatttt gaaagagata gcccatctga tctcgaaaat
    32761 actcacgagt gggaatggtt tttaaatctt tcccctgata gagaaccctt cctccatcta
    32821 tcttttctag ctttcccaag atattcagca gcgtcgtctt tccgcttcca ctctttccga
    32881 ttaaggcata aaccttgccc ttctccagct tgagattcaa accagagaaa atcgcgcggt
    32941 cattaaattt cttttccaat ccttgaatat caatcatctt attttccttt caatactgcc
    33001 atgctaccag cttcttcttt tttgtcctgc cttactaaaa tcaagagggc gttaagcgta
    33061 aacaaagcta caactagagc gctaatcaaa ccatctcttg tcaaaatact agataggact
    33121 aggccaagca agagaactcc tccttgcgcc agtaaatact tgccatgaag ctcatagatt
    33181 gtcataccag caagacgttt aatcataagc tcccgtctga actgctcaaa atagagaaga
    33241 ttcatggaat caaataacaa gatagccgta gacagggtca aaatcgtccc aataaggaga
    33301 gagtaaaact ccacccgttt gtcatttagt accttggcaa aaaatagctg gctttttacc
    33361 aagtaagaaa ccttgtgata cagaccttgc tcttttagag ctgtgatgct gtctccatat
    33421 cgatctagtt tcaaggcact attagccgta gttccccaca acatccctgc aacaggtctt
    33481 gttccagtcg cttgcggcgt tagaactaca ataatcggat cttttaggta ctggcgtgtt
    33541 gtcttgtacc catcattata gaggaaacgt tcctgtcctg tttctgtaaa agctagcctt
    33601 acctgtggga ggtagtgttt ctgactcgtc acttctacac tttcagatga aaagttttgc
    33661 aggtaatctg taaacaatcc ttggtagtag acagactgct ctcgcaagct ctcaggcaag
    33721 atcagcccaa actctccctc agacaagttt tgcatcttgt ccataaactc tgaagaaacg
    33781 gtaatctttt cttctatcag atagcgtggt gagacataga taacattccc tgacggtgtg
    33841 tagtcactga gacgattgcc atctaggtcc acttctgctc catctgagaa attgtcaaca
    33901 ttgctcataa tataaaaaga gtctgtattg gctaaccgtt cttcagtaaa tgtctgccac
    33961 tcacgattat ccttacgcgt tccttcttca tcggcaaatg cactagacca accaaaggat
    34021 agacggtaac ggtctgagga ctggctccat ttattgctag ctctctccat ttcctgcatt
    34081 tcacggtagt ggggtaggag agctgtcgca ctcgatccga ccaccaatac agctaagagt
    34141 tgccccacca tcatcaatgt catcatacgt ttgagaggga gtttcccttt caatagatcc
    34201 accagactat tttcctgtaa accaagtaga tagacgacac ttagtaaggt agaaatccct
    34261 gccaaggtca atccataaag tagaagagca atgatgacca gttgcaccgt tgccataaac
    34321 aaggcacctt gataccagag aatccccaat cccaaaagac tggataccag cactgagcag
    34381 ataagctgtc tcacatcttc taacactggt ctgagagcaa ttccaaacaa gctctcacca
    34441 gctattaagc gaatccctgc ctgacgaagg gatttgatcc gataaatcag ggtcagactc
    34501 ataaaggtca gcagaaaaat agctaaactc agtagcacca taggggtggc cgccaatagt
    34561 aagactatcg aaaatggatc ttctccattc gaaacaaagc cttgatagcc aagttctttc
    34621 aaggtggtct gtaagctcac tccatccaaa cttcctgaca caatcaagta agaccctaat
    34681 aaatcactcg tttctgcact ctccttggag gaaatggtca gaccttctgg aagctctccc
    34741 tcaccataag tggcataggt aaagcgtgtt tctccagctt catttggctc aacgattcga
    34801 cgggcgatga gactattatg ctctaccgcc aagtgactca aagcctctgt cacctcttcg
    34861 cgactagcta ctggttgatt tgtcatcatt cctgtcacat caacagaagg atagttacca
    34921 aataaaatct cttctcgatg gtcaacggaa gttatcacca tataaagcga taccaatacc
    34981 attgaaatca aaataaataa acgtttcatc atttcctcca aaaagttact tggtcactta
    35041 gtgagaaggt gaccaagtaa agctaaacaa tagggctata ccattctaaa cattatagta
    35101 gaatgcaact ttctctcccc aagaagtacg aatccatgca cgagaagtcc ctccagcacc
    35161 agcatatccc ttgtcagatg aaccagtttt tctgctaaca actgttgaac tatggtattt
    35221 tgaagggtga tagtagtttg agtaagcacc accgttacct gaaccataag tccaaacacc
    35281 accttctggg tattgaacgg ctgcagaggc aactgctaca cttccaccta atgttgctgc
    35341 aatgctcgct acaagagcta attttttaac tgtttgtttc atattgatac tcctctattt
    35401 ttgattacaa gattgttgag ttacttgctc ctctttccat tatcaacgtt gatactatta
    35461 tattaccaaa tgatattcta aaattatttg tttttctcta aattgtcaaa atagcaccct
    35521 gaaatgaact tttatagtca ttatactcca tctttatcac actatcaagg acatcaatgt
    35581 ccttaaaatc taactatttc atattatttt tagcttatat ctaaaaaaac agttctaaag
    35641 acgaacttta gaactgtttt gatacgagga caaggacatt tttgtcactg attcactatg
    35701 tttataaata ttttttgaca tcctcttgcc attcctctgt caatttcttt actagaaatt
    35761 catctccaat tagctgcgca aatgttttag aagactgata aaatgattca gctaaactaa
    35821 aatctctttc ttctcttaat gcttgcttcc acaaaaacat aaatacaaga ggtttctttt
    35881 gataatcttg agttttatcc ataatttctt gtaaacacga tagataatca ttaaaacaat
    35941 cagttactcc taccttttca agacaacaca ttcctgaaat aagagtatct ctaattataa
    36001 agaagttttc taatttagtt acctttttct gttccaaaag aaagagcatt agcttttcga
    36061 tttgatttat aaatttggca agatcttttc taatgagcat ttgataaaaa aacaaacgaa
    36121 tcaacaacat atcatttatt gaataagctt ctttttctat aatctgaggc atatgttcct
    36181 caagtattaa ctctgcatat tcaggccttc tagttctaga agtatccaaa cacgcctgca
    36241 atacttcaca tgctaattgt tcttctttag gaagattatc aaagtatgtt ttataaatct
    36301 cttccaaaca cgcttccttc ttatcgtact cctcttcttt accatagatt ggttcacgta
    36361 aaatctggta tttcaattct aaataagcag aaggaagagc tgaaaaatcc ggcataaggc
    36421 tataaactgg aactcctagc cggcgagcaa tatagtctaa cttgggcaaa ctcggttgcg
    36481 aagctccact ttcaattcgc gacagttgac gaactgtcag ttcttgctca tctccacaaa
    36541 aatctggacg actaactcct ttttctatcc gaatctcttt aattttttga ccaattaaca
    36601 tcagacgcct cctcttctac ctgcttcgaa aaatactgtg aaacttaaaa aattgtaacg
    36661 gctacatctt tcctactcat aagatttcat agcttcttcc acttctgcca aagtaacccc
    36721 aaacaactct aggcgtttga ggagttgctt gccgttagaa tagccgattc ggagagtctc
    36781 tccgagatat tctctacgct tacggctgtc tgcccctgct agaaaaccaa ggcgaatcaa
    36841 atcgctacga ctaatgtcaa actgactctc atgttcaaat tgttctgtca cctgagctag
    36901 agccgttttc aggtcttcat agctggcatg ctcaattccc agagaacgcc ccttggtctt
    36961 ggacttggga acagcttcat ctcgcttgag aaaggcatgc tgaactgttg gaatggccgt
    37021 cataatcatg cgccgaatcc gctccccatt aaaatctggg tctgtaaaga caatgactcc
    37081 atgacgttgg tgtagacgct gaattcgctc tatatcctga tcattgatgg cagaacctcg
    37141 tgtctcatag gtctccacat cgaaataacg cttgagattg accgtatcat cgcgaccttc
    37201 aaccacgata acttgagaaa ttttctcttt cattacttgc tgtccaatcc aaaaatgcgt
    37261 tctgcatttg cagtcgttgc cgccgctagc tcttctgtcg tcataccacg caagtcagcg
    37321 ataaagtcca ccacatagcg agtataggct gttttatttt cacggccacg cttgggtaca
    37381 ggtgctaagt aaggcgcatc tgtttccacc aacatcttgt ccaaaggtaa ctctttagct
    37441 gcttcttgga ggtcagttgc cttcttgaaa gtcaccactc ctgagaagga aatggtcata
    37501 ccaagatcca caaacttctc tgcccactca agcgtccctg aaaatgaatg catgatacca
    37561 ccacgaggac caacgccctc actcttgata atctcatagg tatcttccag cgcatcacgg
    37621 gtatggacaa caaaaggcaa atccaagtcc ttagatagct gaatctgacg gcgaaaaacc
    37681 tgctcctgca cctctttggg cgctgtcatc caatggtagt ctaagccaat ttcacctaaa
    37741 gccacaacct tggaatgttt taacttatcc aacaagtaag cctcaacttc ctctgtataa
    37801 gtaccagctt ctgtaggatg ccaaccaata gtcgcataga gctgctcata ctcatctacc
    37861 aactccaagg catgctcaat cgtcggttta tcaaaaccaa caatattcat ctgtgtcaca
    37921 cccatctcat cagccaaggc aatttcttct gcctcacgac ctgcaaattc ttctacattc
    37981 aagtgtgtat gtgtatcaaa aatcatctct tctaacctcg ttttctatct tctattatac
    38041 caaaaaagag gaggggcacc taatttttcg gtttcccctc ctctcttcaa tagggagcta
    38101 ttctgctatc ttttctatcc gatattgccc atctcctatt ccacagttag agacagaaga
    38161 gattggctac attgatagaa tattcctatt cagctaaagt atgtgattat cttgggttct
    38221 cacttttctc acccatcact aggattgatg acttctcaaa atccctctta taatctaact
    38281 ttaagtagag aatagaattt tctagctgtt ctattttcta cgtctagtct ttcttcctag
    38341 caaagcaaaa ccacttgttg ccaccactag accaagacta gcaagtgctg cacttgatgc
    38401 ttaacctgtg ttaggtaatt gtttgccagc aggtgactgg taggtaacat cttgcattgt
    38461 tcctgcagtt gcttgcggtg cacttgttgt cttaggagca gcattaggag ccactggtct
    38521 cttaggaact gtaaccatgg caatggcttg cactccaatc gtttctgctt ccttcacatc
    38581 ttccatagtt ttcgcatttt caatctcttt gagagctgct tgtttttctg cttccactct
    38641 tgctaaaagt tctgcttttt ctttatcaga aagcggtgcg cctttgattt catcctgctt
    38701 gttcttggct gctttttcaa tggcattttt agctgattct ttttcagtag ctaattgctt
    38761 agaagcttgt gattgagctg agttttgtga acctgtgtga gctgatccac ccgcctggct
    38821 accaccgcct tcattcgcat tagcatctga gcttccagta ttgcctccct tatctccacc
    38881 tggtgtattt tctagcttat actctggtgt agcttcttga gttacagatt cacctttcgc
    38941 agattgttga actgtatctt ttcctagaat cgtcactact gaaccatctg ggaatgtgat
    39001 tgtcgctgtg ccatctccag ctacttcaac tttatagtaa aataaaataa taacaggaca
    39061 aatcgatcag gacagtcaaa tcgatttcta acaatgtttt agaaacagag gtgtactatt
    39121 ctagtttcaa tctactataa catcgcttcc gaatccattt gcatctactg aactttttta
    39181 caaaaataaa aatcagtaga ctcgagttcc tactgatttt tgatagaatt cgtttggatt
    39241 atgcagccaa aactttgcgt cctttacgac gacgagctgc caatacgcga cgaccatttt
    39301 tagttgacat acggttacgg aatccgtgtt tgcgcgcacg acgaagttta cttggttgat
    39361 aagtacgttt cacgatgaat acctcctcat agattttgta ttcgtttagc cggctataaa
    39421 gtgatcagtt actaaacata ctttactatt ctatctgatt ctttcacttt tgtcaatagc
    39481 tctaggcaaa tgtttccagc tttttcgaat gcaagcccta tctacgatac tgtttcaaga
    39541 aacgagtcat cttttcttgg atttgggcta actcatcaac acgaggaagg taaacgatac
    39601 ggaagtggtc tggttcctgc cagttaaagc ctcgaccatg aaccaagaga accttttcct
    39661 gcttcaagaa atcaaggaca aactgctcat catcatcgat acggtacata ttgcggtcga
    39721 tttttgggaa gatatagagc cccgccttgg gtttaacggc agacaaacct ggaatatctt
    39781 gaatggcatt atagatgaaa tttctttgct cgtagattcg tccaccagga agaagcaatt
    39841 catcgactga ttggtgaccc cccaaggaag tttgtacgac ttgttgggcc aaaacgttag
    39901 agcaaaggcg catattggac agcatattga gcccttcgat atagccctta acatgagtct
    39961 tagggccaga caagaccatc caacccacac ggaaacctgc tatgcggtgg gattttgaca
    40021 gaccattcat gctgacacag aagacatctg gtgccaagct cgccacaggc gtatgcacat
    40081 gtccgtccat taccatgcgg tcatagattt catccgcaaa gataatcaaa tcgttttgac
    40141 gggcaatctc aataatctcc agcaagagtt ccttaggata aagggctcca gttgggttat
    40201 ttggattgat aaggacgatt gccttggtat tggaagtgat ttttgacttg atatcgtcaa
    40261 tatctgggta ccattcaaca gcttcatcac agatatagtg aacggcattt cctccagcta
    40321 ggctgacagc cgccgtccat agaggatagt ctggcattgg cactaagacc tcatccccat
    40381 tgtccaaaag cccctgcatg gacatgacaa tcaactcact gacaccattt ccaaggtaaa
    40441 tatcatcaat atctacgttt gggaatttct tcagctggca atactgcatg atggccttac
    40501 gggctgagaa aatccctttg gagtcagaat agccttcact atcacgcgca ttcataatca
    40561 agtcatgaat gacctcgtct ggcgctgtaa agccaaattc tgctggattt cctgtattca
    40621 gacgtaaaat cttttctcca ttcgctcgca tccgcatggc ttcttccaat acaggaccac
    40681 ggatatcata agcaacatgc tctaacttac tagacttgtt atattctttc atcttgtttc
    40741 ctcaattcat caggatagta acattatacc actagaaaga gaatgcgaca agtttactga
    40801 aatgtgttac tagtttcgta tagaagaaaa accttgctaa aggcgaggtt aagaacgtag
    40861 agatagcagg tattttgcaa agacacatca cataccaaaa gataaaccta gaaaaagcaa
    40921 gcctagaaag gagtcataaa gaagattaaa catgaggaaa agcccccata tcatcaagta
    40981 atcccaacgc ccacttcgtt ttgcaactag aaataagatt agatggtaga gtacatgaaa
    41041 gactaaaaat ccaataaaat ctggataaag aagcggtacc agactctcta attgccagat
    41101 catgatcact tctaccaaga ctgaaactag gataattcca taataaagga aatttgattt
    41161 ttgaaaagag tttttcatca ttctcccttc cttcacttct ttcctgctat cctttctttt
    41221 attttatcat atatttatat agattttaag ccctaatatt cttttgaaaa atactttttt
    41281 tgctttactt ctatagtgaa aaggtttaca attatagtaa gaaaatttca ggaggtttca
    41341 ttatggcaca acgttaccaa aatatcatgg tcgcaatcga tggttctaag gaagcggact
    41401 tggcttttgt caagggagtt cattctgctc tacgaaacga cgctaaactc accatcgcac
    41461 atgtcattga cacacgcgct ctccaaagcg tatccacctt tgatgctgaa gtttacgaag
    41521 aactccaagt cgacgctgaa agtctgatga aagagtacga aaaacgtgct aaagatgctg
    41581 gggtagcaga tgttcatatc gtcattgaaa tgggaaatcc aaagaccctg ctagcacgta
    41641 ctattccaga tgccgaggaa gtggacctca tcctcgttgg cgcaactggt ctcaacgcct
    41701 ttgaacgcct cttggtcggc tcttcatctg aatacatact ccgccatgct aaggtcgatt
    41761 tgctggttgt gagagaacaa gaaaaaacct tataatcaca aagaaaagga gcccctagct
    41821 cctttttgtt tacgatttat ttctctcttt atggcgttcg taagccttga gctggcgctg
    41881 cagttccttt ttaatagcag gttctggagc atatttttct tcccaattat ctggttttaa
    41941 gattttatgg gtcactggat caaaatgagc cttgccatct ggaaaaattt tccccatatt
    42001 ggcctgatgg acaatatcaa aaatacgttc tgggtccacc cccatcaaga caaaactgcc
    42061 gtaggcgaag taaagcgtgt caatcaaggc atccacttgc cctatcaaat cttgctgagc
    42121 aggtgtcttc ttggctactt tacctgctgc cttatcaagg gcctgatgaa gttgcgatac
    42181 agcttgacca aaatcttctt cagaaggact ggctgctcga acaaactcca ccaattcttc
    42241 tattttaaaa ccagccctat gggttgcacc ctctaaatcc caagctcgag gttcttcttg
    42301 ggttcgttca tccatcatgt ggtggaaggt cttgacctta ttgaaatgat agtcacggct
    42361 gacaaagact ttttctgaag acaaaacacc aaaatgttcc aaggccttgt ggatcccatc
    42421 ttgttgattg ctagtggtaa tgtgcttggc agcttccttg acactgctac taccatttcc
    42481 catagcgacc gacataccga cgccagccag catttcaagg tcattatctg agtcaccaaa
    42541 agccatgact tggttgaggt caaagccata ttctttccca actcggcgaa tgccttctaa
    42601 tttagaattt ccctgattga tgacatccga tgcaaaagga ttgctacgtg tcaatttcaa
    42661 gtcttcaaaa tcagctgccg ccttctcaga ttcttctggt gtcatcagca tcaaaacttg
    42721 gtagataggc tgattcatca ggtgaagcag gtcctcttcc ttttggggaa caaccttgct
    42781 gaccatgcga ttaaaagact gactcaccgt ccgagttaaa acagagggaa cgaagcgact
    42841 aattcgttgg gaaaaagaac ccagaccaaa ggacatgatt ttagaaccca acatggcatc
    42901 cttggtccct agagcaatct ccgtgccctc ttttttagca tagctaatta gatggcgcaa
    42961 atgtaacttg gaaatagggc tcgtgaacaa gactctgtct ttactaaaga tatactggcc
    43021 attataggtt accgcaaaat ccagatccaa atcgtccatc aattccttaa caaaaaaagg
    43081 tcctcgccct gtcgctacgc caactagtac cccttgttct ttgacaatct taatcgcgtc
    43141 cttagtggat ttcaaaacac tcttgcgatt gttgaccaag gttccatcga tatcaaaaaa
    43201 aacagctttg acttccatcc tattccaatc tccccttttg tgatacaatg attataccac
    43261 atttcagaaa gagttagtaa atcatgccta agaaaatcct tgttttacat acgggtggaa
    43321 ctatttccat gcaggccgat gcttctggcg ctgttgtgac gagttcagat aatcccatga
    43381 accatgtgtc caacccactt gaaggaatcc aagtccacgc cttggacttt tttaaccttc
    43441 caagtcccca tatcaaaccc aaacatatgc tggtcctcta ccagaaaatt aaagaggaag
    43501 cagataacta cgatggagtg gtgatcacac acggaaccga tactttagag gaaacagcct
    43561 atttccttga taccatggaa gttccccata tgcctatcgt tctaacagga gccatgcgta
    43621 gctccaatga actcggtagt gatggtgttt ataattacct aagtgcttta cgagtggcca
    43681 gcgatgacag ggctgctgac aaaggagttt tgatcgttat gaacgatgaa atccacgctg
    43741 ccaagtatgt caccaaaaca catacgacta atgtcagcac cttccagact ccaacacatg
    43801 gccccctcgg tctcatcatg aaacaggaaa tcctctactt caaaacagct gaacctcgtg
    43861 ttcgctttga ccttgatcac atacaaggtt tagtccctat catctcggct tatgctggta
    43921 tgacagatga gctgattgat atgctggatt tagaacactt ggacggtttg attatccaag
    43981 ccttcggagc tggtaatatt cccaaagaaa cggctaaaaa attagaaagc cttctgcaaa
    44041 aaggaatccc agtcgctctg gtatcacgat gctttaacgg tattgccgag cctgtttatg
    44101 cctaccaggg tgggggcgta cagttgcaaa aagcaggcgt tttctttgtt aaagaactca
    44161 acgcccaaaa agcccgcttg aaactcctca tcgccctcaa tgccggacta acaggacagg
    44221 ctttgaaaga ctatatggaa ggctaatact cttcgaaaat ctctgcaaac cacgtcacgt
    44281 cgccttaccg tatgtatggt tactgacttc gtcagtttca tccacaacct caaaaacatg
    44341 ttttgagctg acttcgtcag tttcatctgc aacctcaaag cagtgctttg agctgacttc
    44401 gtcagtttca tctgcaacct caaagcagtg ctttgagctg acttcgttag tttcatctac
    44461 aacctcaaaa acatgttttg agctgacttc gtcagttcta tctgcaacct caaagcagtg
    44521 ctttgagctg acttcgtcag ttctatctac aacctcaaaa cagtgtgttg cgcagccttt
    44581 aaccagccgc ctagtccgct ctatggtatt cattaagtca acatctcttg tttaagagca
    44641 caaaatcagg aaatcttctc gattccctga ttttttctat ttacgttttc gtgttgagct
    44701 acgttctgtc aaaccatgag gtaagagaac ttcacgttct tccaactctt ccttatgcat
    44761 aatcttggtc aacatacgca tactaatggc accaaggtca taaagaggtt gggcaatcgt
    44821 tgtcaagttt ggacgggtaa agcgtgagat ttgtgaatca tcactagtaa taatttcaaa
    44881 atcttctggc acagaaacac ccttatcagc caaaccgttc aagactcctg ctgccaactc
    44941 atcacctgtc acaactgctg cagttgcatt tgatgaaatc aaacgctctg ctaaggcgta
    45001 accatcatca tagctatatt tagattcaaa taccaaaccc tcactataag tgattcctgc
    45061 ttttttcaag gtttccttgt agccaactaa acgaacctta ccattgatgt catccactag
    45121 cggaccgcta acgaaagcaa tacgctcatt ttctttagca aggtaactca ctgcatcaat
    45181 tgttgcttgc ttatagtcaa tattgacact gggcaactgg tgctcaacat cgacagttcc
    45241 tgcgagaaca atcggagtac gtgaacgcga aaattctgag cgaattttat ctgtcaagtg
    45301 ataacccata tagataatgc catctacctg ctttgaaaag agggtattga caacagaaac
    45361 ttctttctcg ttatcttcat cgctattagc taggacaata ttgtacttgt acatttctgc
    45421 aatatcatca atccccttag ccaaactcga aaaataacca ttggtaatat ttggaatcac
    45481 gacaccgaca gtggttgtct ttttacttgc aagaccacgc gcaactgcat ttggacgata
    45541 atccaaacga tcaattacct ctagcacttt tttacgggta ttctctttta catttttatt
    45601 gccattgacc acacggctga ccgtcgccat ggaaacacct gcttcacgag cgacatcata
    45661 aatggttact gtatcatctg cattcattcc ttttcctgtc ctttctatct cacacattct
    45721 tttacaagta gaggtactga ttgaagctct atatctactt acaaaagtga agatgtgaaa
    45781 atttcgtttt catatttcta cttattccat tctatcacta attgtaaaca ctttcaagtg
    45841 ttttttgaag attgattgaa aaaatttcat agaaaaccta ggtttagctc cttgctacca
    45901 ccttagacta aacaaaaagg aggaaactaa gccctcctaa agttatagta aaatgaaata
    45961 agaacaggat aaatcgatca ggacagtcaa atcgatttct aacaatgttt tagaagtaga
    46021 ggtgtactat tctagtttca atctactata ggtattgttc cattcaatcc catcaatttt
    46081 agcacataat ggttttcgag atatgatagt attacaacca accagattct tttgcgatat
    46141 tagctgcctc tgttcgatta cctgcatcta gtttcgaaag aatattagtg acatagtttc
    46201 gaatagttcc gtttgagagg taaagctgat ctgcgatttc ttggttggat aagccccggg
    46261 caattccctt taagacagca atctcttgtt ctgtcaacgg attgggacgg gtcatcacca
    46321 cttccatcaa ttcaggtgaa tactccttgc gtccttcgag gacggtgtgc aaggtttgca
    46381 tgaggtctgc aatgcttcgt tcctttaata cataagcatc cactccagcc ttgaccgcac
    46441 gttcaaaata cccagcacgc ttgaaggtcg tcaccacaac cacctttgtt tcaagtcttt
    46501 ctgatcgtat ccactccaag acttcaagac ctgtcttaac aggcatttct acgtcaagga
    46561 tggcgatatc tacagactcc ttttctaata gttggattgc ttcttgccca ttcttggctt
    46621 gaaggacaga ctccacatct ggttgaagcg tgagcaattg acacatggca tctcgcaaca
    46681 tactttgatc ttctgcgact aatactttca tcttagtttc tctccttata aggtagtcga
    46741 acttgcactt ccgttggatg tttctgactg attacactta cttctcctga aaatggaaaa
    46801 acacgatctc ggactgtatg gagctcatct ccttttagaa aagcaaagcc gcagccatca
    46861 tcacttactg ttaaaatgag ttccttctct gtccgttcta attttaagta agctttagac
    46921 gctttggcat gtttgatgat attggtcacc aactcaagca aaatcataga agccattgaa
    46981 tccaattctt gagtaaggct agcagtatct agttggttat ctgtttccac ctcaattcca
    47041 gcaatttcta acatcttttt cacagtctct agttcggatg tcaaagttct agacttaaga
    47101 ttttccacaa tggttcgaac ttcacacatt gattctttgc taatttgctg tatttctctt
    47161 aattcctttt ccacttgtgg ataagcctgc atttgaaata gctgcaaggc tagatctgtc
    47221 ttgacactaa gcatagcaaa ggtatgtccc agactatcat gcaaatcctg accgatacga
    47281 ttgcgctcat tttctgctag caatagattt atctgggtat tttgcttgac ctgagcttct
    47341 ttcaaatcct cgacaaggcg aatccgaacc gagcccaaag tcatcaaatc gacaaaagta
    47401 agaattccaa gcatatagac tagaaactca acttcgattc tctgaaaaat caaaagtcgc
    47461 cccacaacaa ggacttgagc aaggagaaat gtccatacat gtaaagactt taaactacgt
    47521 acgccaaaat gataacttaa gagattggaa agaaaaaaga aaaaccagat ataattcaca
    47581 gcaacaaagg cggtattccc agctacatag accagcatga tcccccagaa aagccaagat
    47641 aggcgctggt tcttagttat taaaacaccc aaatacgtca ctacaaatag aatatcaacc
    47701 aataaatgcc aagcaggaag ctccccaatc actacaggta ggatgggaaa aagcataaaa
    47761 atcaaactgg cccaaaacat atagtgtatg cttttcagtc tttcaagcat taagcattct
    47821 ccttatgacc ttgaaggtaa atagtcaaac caaataaaac tgctgaaaaa acaagtaaat
    47881 aaactgtggc tgatagattg atgccaccct cgtttaagaa ggtcttgagc aactccatca
    47941 attgataggt cgggagacac ttaccaactg cttgcatcca gtctgaaaat aaagagacgg
    48001 gcatccagag tccacctaaa acagccaagc caaggtaaag aagattgccc acgacagaca
    48061 tcaactgact agttggtaag agcgtcaagg tcaaaccaat cgctacaaat gcaatacttc
    48121 ccaaaatcag caagagcgcc gccccaatcc aatttccaag agacatgtcc acacctctta
    48181 caaaatgccc aactgagaaa acaaccagaa ttgagaccat ataatcaacc agcatacttg
    48241 ttatctttga tagataatat tctaccatat ttacagggct atgacgcaat gttttctgcc
    48301 aattgttgat cttgtcggta tgtaagacag ctggaaatga gaagatagct gtcgacatca
    48361 tggaaaaagc cgtcatggag ataagataat cacgcataaa attcgcgggt ccacctggtg
    48421 tgttctggta catgccagaa aagaatagat agaaggctgt cggcatccct acggataata
    48481 aataatagat tgattgccgt ttggtcaata gaaattctat cttatttaat gctatccatt
    48541 gtttcatctt agtcatctcc cttctgagtt tcttcaaaga ttgtatccaa caaactacga
    48601 ttattaactt cgatttcttg catcctacat cctgcttggg ctaatagttc ccagaaagca
    48661 tctgcttctc gagtgactac ttgtaaagaa tcttgcttta gggtccagtt ttcaaccaaa
    48721 tttgactgct ctacgacttc cttgtaagct ataggaagga taaagtgctt ttctatttcc
    48781 tcgctacgca tagctagagg cgtcgtatct cgaatcaact ctcccttatt taagagcaaa
    48841 atgcggtctg cagtgtgttc cacttcctca atataatggg acgaatagag aatggtgact
    48901 ccttgcgctt ttaactcctg gacaatttcc caaaaacgtt gacgagttga ggtatccatg
    48961 gacacagtag gctcatctaa aaaaacaatc tttggtcgcc caattaaggt caagacgaaa
    49021 gaaaagagac gcttttgacc gcctgataat ttttctgcca actgttcttt ttgttgcttg
    49081 acaaactgca atagttgatc aatttcctga ttgctcaaag gatttggata gatacgttga
    49141 aagaaagcaa tcaactcttt aacttttaat ttctgaacga ttacattttc ttgagggagg
    49201 taagccctag tatagtctaa cttagaactc gtcactggta aaccttgaat agatacttga
    49261 ccgcttgtga ccagtttatc tccaagcaaa caatctaaga gtgttgtctt tccggcacca
    49321 tttggaccaa ttaaggcaac acattcacca tcgttgattt caaaggagat gttccgcaaa
    49381 atttccttgt cttttatttt cttactcaat ttttcaactt taaccacagt catgagactc
    49441 tcctttcaac cactccattc ccatagggaa aacgacgaaa atcataaatc caaaccccaa
    49501 agcaccatga atgaattggc gaagcaaggc ttgatcaaac caatcagtaa acatttccac
    49561 taaccatacc aagagtgaca ggccgataaa gaaatagatg atccctctct tcattcttca
    49621 aactcctttt tcacatctct gactaatttc aaaccttctc taacaagcca agacatgatt
    49681 ccaaagccag caaagacctt ccaaggaaaa tggtagaaat cctcatccaa tcccgaaaac
    49741 atgagatagg tcataactcc tgctactact aaactcattg caataatcat tttatttctc
    49801 atctcttctt cccccatttc atactacaat tatagtcttt taaaatcaga ggaggtagaa
    49861 gcttctgtca ctagaaaata tgacaaatgt cataaaaaat tctgttcaaa acaagcaaga
    49921 tacactatac aataaaacac aatcagaaaa atctaaggca acttcctcaa aagagatatc
    49981 aaactcaatt ca
//
LOCUS       DAVGJL010000003        45786 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.003, whole genome shotgun sequence.
ACCESSION   DAVGJL010000003 DAVGJL010000000
VERSION     DAVGJL010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 45786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45786
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.003"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            80..1024
                     /locus_tag="VVE42_000116"
     CDS             80..1024
                     /locus_tag="VVE42_000116"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HEU3072389.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWVNHAVYQETPFDWRKIEDDASAYGYL"
     gene            1033..1710
                     /locus_tag="VVE42_000117"
     CDS             1033..1710
                     /locus_tag="VVE42_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEU3072390.1"
                     /translation="MTYLDAFKSGTLVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEMSPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELFFDASLALERL
                     DDLFGAVRSSSDQLTPQNQLKDLVETFQQELGRLLTPFEIEDLTKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFRN
                     AMDLWKD"
     gene            1808..2566
                     /locus_tag="VVE42_000118"
     CDS             1808..2566
                     /locus_tag="VVE42_000118"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEU3072391.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVASKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNLKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLTAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            2604..4109
                     /gene="lytC"
                     /locus_tag="VVE42_000119"
     CDS             2604..4109
                     /gene="lytC"
                     /locus_tag="VVE42_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
                     /protein_id="HEU3072392.1"
                     /translation="MSVTFFIGEERLKIKIGLASICLLGLATSHVAANETEVAKTSQD
                     TTTASSSSEQNQSSNKTQTSAEVQTNAAAHWDGDYYVKDDGSKAQSEWIFDNYYKAWF
                     YINSDGRYSQNEWHGNYYLKSGGYMAQNEWIYDSNYKSWFYLKSDGAYAHQEWQLIGN
                     KWYYFKKWGYMAKSQWQGSYFLNGQGAMMQNEWLYDPAYSAYFYLKSDGTYANQEWQK
                     VGGKWYYFKKWGYMARNEWQGNYYLTGSGAMATDEVIMDGARYIFAASGELKEKKDLN
                     VGWVHRDGKRYFFNNREEQVGTEHAKKIIDISEHNGRINDWKKVIDENEVDGVIVRLG
                     YSGKEDKELAHNIKELNRLGIPYGVYLYTYAENETDAENDAKQTIELIKKYNMNLSYP
                     IYYDVENWEYVNKSKRAPSDTDTWVKIINKYMDTMKQAGYQNVYVYSYRSLLQTRLKH
                     PDILKHVNWVAAYTNALEWENPYYSGEKGWQYTSSEYMKGIQGRVDVSVWY"
     gene            4605..5123
                     /locus_tag="VVE42_000120"
     CDS             4605..5123
                     /locus_tag="VVE42_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEU3072393.1"
                     /translation="MVELKKEAVKDVTSLTKAAPVALAKTKEVLNQAVADLYVAHVAL
                     HQVHWYMHGRGFLVWHPKMDEYMEALDGQLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEAGEYRNVEESLERVLVIYRYLSELFQKGLDVTDEEGDDVTNGIFAGAKTETDKTIW
                     MLAAELGQAPGL"
     gene            5253..5759
                     /locus_tag="VVE42_000121"
     CDS             5253..5759
                     /locus_tag="VVE42_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEU3072394.1"
                     /translation="MTKKIVAIWAQDEEGVIGKENRLPWHLPAELQHFKETTLNHAIL
                     MGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATFQDVQSVLDWYQDQEKNLYIIGG
                     KQIFQAFEPYLDEVIVTHIHARVEGDTYFPKELDLSLFETVSSKFYAKDEKNPYDFTI
                     QYRKRKEV"
     gene            5759..5929
                     /locus_tag="VVE42_000122"
     CDS             5759..5929
                     /locus_tag="VVE42_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3072395.1"
                     /translation="MERSLFGLFTAFLCFICFLAGAQAFRKKRYGLSILLWLNAFTNL
                     VNSIHAFYMTLF"
     gene            5961..7193
                     /gene="clpX"
                     /locus_tag="VVE42_000123"
     CDS             5961..7193
                     /gene="clpX"
                     /locus_tag="VVE42_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEU3072396.1"
                     /translation="MSTNRKNDMMVYCSFCGKNQEEVQKIIAGNNAFICNECVELAQE
                     IIREELVEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKSENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELEFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            7202..7789
                     /locus_tag="VVE42_000124"
     CDS             7202..7789
                     /locus_tag="VVE42_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEU3072397.1"
                     /translation="MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHDPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL"
     gene            7800..8180
                     /locus_tag="VVE42_000125"
     CDS             7800..8180
                     /locus_tag="VVE42_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU3072398.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            8232..9122
                     /gene="rapZ"
                     /locus_tag="VVE42_000126"
     CDS             8232..9122
                     /gene="rapZ"
                     /locus_tag="VVE42_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEU3072399.1"
                     /translation="MTKKQLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKRLVQDLSKN
                     WSVNEGHRDKDHRKETVNRS"
     gene            9119..10096
                     /locus_tag="VVE42_000127"
     CDS             9119..10096
                     /locus_tag="VVE42_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEU3072400.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDFRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGTEVAGESHIVDHRGIIDNV
                     YVTNALNDDTPLASRRVVQTILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQEYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            10093..11004
                     /gene="whiA"
                     /locus_tag="VVE42_000128"
     CDS             10093..11004
                     /gene="whiA"
                     /locus_tag="VVE42_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEU3072401.1"
                     /translation="MSFTVAVKEEILGQHHLSWHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHFYEIKSEIRHHQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAILSDEEAGRAYLCGAFLANGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(11038..12006)
                     /locus_tag="VVE42_000129"
     CDS             complement(11038..12006)
                     /locus_tag="VVE42_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU3072402.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEEFAITTS
                     KGSHLTKTVIIAMGGGAFKPRPLELEGVEGYENIHYHVSNIQQYAGKKVTILGGGDSA
                     VDWALAFEKIAPTTLVHRRDNFRALEHSVQALQESSVTIKTPFAPSQLLGNGKTLDKL
                     EITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYA
                     IGDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL"
     gene            12142..12999
                     /locus_tag="VVE42_000130"
     CDS             12142..12999
                     /locus_tag="VVE42_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEU3072403.1"
                     /translation="MNFQQLSNLQYWTSLFASPWTIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYTSTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERL
                     LGGWKHEKK"
     gene            12986..13765
                     /locus_tag="VVE42_000131"
     CDS             12986..13765
                     /locus_tag="VVE42_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEU3072404.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSTSARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEVSITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            13789..15141
                     /locus_tag="VVE42_000132"
     CDS             13789..15141
                     /locus_tag="VVE42_000132"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEU3072405.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSAIGLAFDGDSDRLIAVDENGDIVDGDKIMYIIGKYLSEKGQLA
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAVQLTKIMKETGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KAIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTTEEVDYYVDTITDVVRAEIGID
                     "
     gene            15215..15589
                     /locus_tag="VVE42_000133"
     CDS             15215..15589
                     /locus_tag="VVE42_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEU3072406.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWENENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPSELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            15591..16439
                     /locus_tag="VVE42_000134"
     CDS             15591..16439
                     /locus_tag="VVE42_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU3072407.1"
                     /translation="MKLAVFTDSSAYLSAETLQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASELPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNAPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            16716..17483
                     /locus_tag="VVE42_000135"
     CDS             16716..17483
                     /locus_tag="VVE42_000135"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEU3072408.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLTDPDLDLVAVLDPFESESEWQ
                     GIPVFKDKADLAGFEADVWVDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRAQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEVRESIQQGAADEEELIAGARGADFDGMRIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            17495..18679
                     /locus_tag="VVE42_000136"
     CDS             17495..18679
                     /locus_tag="VVE42_000136"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEU3072409.1"
                     /translation="MPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHSPIHDVDIA
                     TSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSAVSFVR
                     SLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDALRIMR
                     GFRFQASLGFALEPETFKAMKTLTPLLEKISVERTFVEFDKLLLAPFWRRGLASMIES
                     QAYDYLPDMASSQDKLNRLFDLETDFTFESSEQAWAALLWALEIENAQSFLKSWKTSR
                     QFAKQVQDLLIILALRENGELSKRDCYRFDIDLLLQAENLRQAQGKEVNPQAITEKYQ
                     SLTIHDKKEIQINGGILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIHAYLRE
                     KK"
     gene            18676..20547
                     /locus_tag="VVE42_000137"
     CDS             18676..20547
                     /locus_tag="VVE42_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU3072410.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKNDYQIGYLTQDPDFDDRKTVLDTVLSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLSKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELDRAGLTEYQGNYQDYVRLKAEQDERDAALLHKKEQLYKQELAWMRRQPQAR
                     ATKQQARINRFHDLKKKVSGSSAETDLTMNFETSRIGKKVIEFQAVSFAYENKPILQD
                     FNLLVQAKDRIGIVGDNGVGKSTLLNLIAGSLEPTKGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDGKIRPFFGHYTDYLDEKAFETDMANQVQKAEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIETLEKRIAAIEEEMQANGSDFGKLATLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(20690..21874)
                     /gene="mntE"
                     /locus_tag="VVE42_000138"
     CDS             complement(20690..21874)
                     /gene="mntE"
                     /locus_tag="VVE42_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HEU3072411.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLAAGHLLHSSSLVAD
                     GFNNVSDIIGNVALLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDVLRD
                     TIQKILSREETVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSKALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLEERF
                     GVFDTDVHIEPAPIPEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            22178..24874
                     /locus_tag="VVE42_000139"
     CDS             22178..24874
                     /locus_tag="VVE42_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEU3072412.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQAVDATEQGLSSSEAEKRLAEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LLEANSLKIEEAALTGESVPVEKDLSVELATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILVIALVTFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVVLANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFDSDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDIAPIDEKVTNLIHTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEDHVLTGAELNELSDEDFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            24887..25678
                     /gene="yghU"
                     /locus_tag="VVE42_000140"
     CDS             24887..25678
                     /gene="yghU"
                     /locus_tag="VVE42_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HEU3072413.1"
                     /translation="MSAYQLPTVWQDEASNQGAFTGLNRPTVGARFEQNLPKGEQAFQ
                     LYSLGTPNGVKVTILLEELLEAGFKEAAYDLYKIAIMDGDQFGSDFVKLNPNSKIPAL
                     LDQSGTEDVRVFESAHILLYLAEKFGAFLPSNPVEKVEVLNWLFWQAGAAPFLGGGFG
                     HFFNYAPEKLEYPINRFTMEVKRQLDLLDKELAQKPYIAGNDYTIADIAIWSWYGQLV
                     QGNLYQGSAKFLDASSYQNLVKWAEKIANRPAVKRGLEVTYTEIK"
     gene            25794..26204
                     /locus_tag="VVE42_000141"
     CDS             25794..26204
                     /locus_tag="VVE42_000141"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU3072414.1"
                     /translation="MEKKIVKDILFLSQVSQPASQEDLYLARDLQDTLLANHDTCVGL
                     AANMIGVQKRVIIFNLGLVPVVMFNPVLLSFEGSYEAEEGCLSLVGVRSTKRYETIRV
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
     gene            26216..27811
                     /locus_tag="VVE42_000142"
     CDS             26216..27811
                     /locus_tag="VVE42_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3072415.1"
                     /translation="MKRIVFELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPRSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVDDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRIYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTK
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESINGVVADLKSAVIKGDTVYFFKVDGKIYKVKASVSDDLPY
                     LENGKTFEGQVGKDNYLKTFKLR"
     gene            27878..28042
                     /locus_tag="VVE42_000143"
     CDS             27878..28042
                     /locus_tag="VVE42_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HEU3072416.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKEKKDKFLSILTSLAGTALV
                     LVAVWLGWPK"
     gene            28121..28849
                     /gene="yaaA"
                     /locus_tag="VVE42_000144"
     CDS             28121..28849
                     /gene="yaaA"
                     /locus_tag="VVE42_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEU3072417.1"
                     /translation="MKILIPTAKEMNTDLPSIEAISLKPESQAVLDALALYSASQLES
                     FYKVSAEKAAEEFQNIQALKRQTTQHYPALKLFDGLMYRNIKRDKLTEAEQDYLENHV
                     FITSALYGVVPALSPMAPHRLDFLMKLKVAGKTLKSHWKAVYDEALKKEEVIFSLLSS
                     EFETVFSKEIRAKMVTFKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
     gene            complement(29126..29674)
                     /locus_tag="VVE42_000145"
     CDS             complement(29126..29674)
                     /locus_tag="VVE42_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU3072418.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALAGKAEVSYLDYSALP
                     LFSQDLEVPTHPAVAAAREAVLAADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGASALQDKFVTVSSVANAGHDQLFAIYKDLLPFIRTQGVGDFTAARVNDSAWADGKL
                     VLEETVLNSLEKQAQDLVEAIK"
     gene            29995..30483
                     /locus_tag="VVE42_000146"
     CDS             29995..30483
                     /locus_tag="VVE42_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEU3072419.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISASFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEQADQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGVSQEKAETVSKEKGAGEIDKIIFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GL"
     gene            30480..31667
                     /locus_tag="VVE42_000147"
     CDS             30480..31667
                     /locus_tag="VVE42_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU3072420.1"
                     /translation="MKLSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAVVNSYFERFYGYSVAPNQVTVAAGAKY
                     SLYTFFMAVVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSAKEDNHFKVTVEQLEAA
                     RTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEKRLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVTDFTTAILEEAEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            31660..32025
                     /locus_tag="VVE42_000148"
     CDS             31660..32025
                     /locus_tag="VVE42_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU3072421.1"
                     /translation="MIDHFEIKVKDLQISEGFYRSFLAPLDYKLAFKTSSLISFLSPN
                     SPHPGGDFWLTQGTQDPVHFAFLAENKEEVQACYEAGIEAGGRDNGVPGYRSEHPIYY
                     AAFMIDLDGNNIEVVCHKE"
     gene            32041..33387
                     /gene="asnS"
                     /locus_tag="VVE42_000149"
     CDS             32041..33387
                     /gene="asnS"
                     /locus_tag="VVE42_000149"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEU3072422.1"
                     /translation="MMTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVMNYPAAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            33540..33830
                     /locus_tag="VVE42_000150"
     CDS             33540..33830
                     /locus_tag="VVE42_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEU3072423.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            33842..34312
                     /gene="ssbA"
                     /locus_tag="VVE42_000151"
     CDS             33842..34312
                     /gene="ssbA"
                     /locus_tag="VVE42_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HEU3072424.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRIFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LESRSVREGHTGGAYSAPTANYSAPTNSVPDFSRNENPFGATNPLDISDDDLPF"
     gene            34344..34583
                     /locus_tag="VVE42_000152"
     CDS             34344..34583
                     /locus_tag="VVE42_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEU3072425.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            34878..36278
                     /locus_tag="VVE42_000153"
     CDS             34878..36278
                     /locus_tag="VVE42_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HEU3072426.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRNSKTGFIPTTIPTVFKQLRE
                     KGILTGIASGRGIFGVVPEIRDLKPDFFVTLNGAYIEDKKGQVIYQHQIEKSYVEEYI
                     SWAKQEGIEYGLVGSHDAKLSTRTDMMSEAINPIYPDLDVDPDFHEKEDIYQMWTFED
                     KGDDLHLPDSLSDKLRMVRWHQHSSDIVPISGSKATGVEKVVEHLGLKPEKVMVFGDG
                     LNDLELFDYAGISVAMGISHDKIKEKADYITKTLEEDGIFDALEVFGMVEKELHFPQV
                     DIETVEGPLATIKTNHGDLRIKLFPEHAPKTVANFVSLSKDGYYDGVIFHRIIKDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNIRGALSMANAGPNTNGSQFFIVQNQHLP
                     YSKKEITRGGWPEPIAEIYANQGGTPHLDRRHTVFGQLADEASYAVLDVIAAVETGAM
                     DKPVEDVVIETIEIED"
     gene            36280..36639
                     /locus_tag="VVE42_000154"
     CDS             36280..36639
                     /locus_tag="VVE42_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEU3072427.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEEKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHHLKKKK"
     gene            36693..37442
                     /locus_tag="VVE42_000155"
     CDS             36693..37442
                     /locus_tag="VVE42_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEU3072428.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVDPYSNLNESEHYLLARHEITTNLEEICHSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            37432..37731
                     /locus_tag="VVE42_000156"
     CDS             37432..37731
                     /locus_tag="VVE42_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEU3072429.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDMRRRLAIHNSGKGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALFKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            37802..38737
                     /locus_tag="VVE42_000157"
     CDS             37802..38737
                     /locus_tag="VVE42_000157"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEU3072430.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRVITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            38824..39336
                     /locus_tag="VVE42_000158"
     CDS             38824..39336
                     /locus_tag="VVE42_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEU3072431.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGVVCQEPADFFESNHLYDLYVYPEMDIQSVKERACRHLGAPYNASFYPDAAGFYCSQ
                     YIAEILPIFETIPMKFGDGEQEISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERNLHDSDF"
     gene            39373..39968
                     /locus_tag="VVE42_000159"
                     /pseudo
     CDS             39373..39968
                     /locus_tag="VVE42_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809149.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
     gene            40069..40263
                     /locus_tag="VVE42_000160"
     CDS             40069..40263
                     /locus_tag="VVE42_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEU3072432.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVAKAKDVV
                     EDAKGAVEGAVEGLKNVFTK"
     gene            complement(40309..41754)
                     /locus_tag="VVE42_000161"
     CDS             complement(40309..41754)
                     /locus_tag="VVE42_000161"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEU3072433.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVIFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWYVAEKDYEVDIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKLLAFTGTKGKTTATYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALT
                     TPESIDLFDMMNQAVQNDRTHLIMEVSSQAYLVHRVYGLTFDVGVFLNITPDHIGPIE
                     HPSFEDYFYHKRLLMENSRAVIINSDMDHFSVLKEQVEDQDHDFYGSQFDNQIENSKA
                     FSFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLN
                     QHPEIQVFLTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGADCYQIIQGKKESYPGDTAVAENYL"
     gene            41860..43482
                     /locus_tag="VVE42_000162"
     CDS             41860..43482
                     /locus_tag="VVE42_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEU3072434.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEHSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRVFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLALGLFVFAVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLWSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKAQADRLRAKFKLS"
     gene            43685..45643
                     /locus_tag="VVE42_000163"
     CDS             43685..45643
                     /locus_tag="VVE42_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU3072435.1"
                     /translation="MKKSKSKYLTLAGLVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRAATSDIVANLVDGLLENDQYGNIIPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTVGV
                     KALDDQTVQYTLVKPELYWNSKTLATILFPVNADFLKSKGDDFGKADPSSILYNGPFL
                     MKALVSKSAIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQESLERNFTAGAYTTARLFP
                     NSSSYEGIKEKYKNNIIYSMQNSTSYFFNFNLDRKSYNYTSKTSDIEKKSTQEAVLNK
                     NFRQAINFAFDRTSYGAQSEGKEGATKILRNLVVPPNFVSIKGKDFGEVVASKMVNYG
                     KEWQGINFADGQDPYYNPEKAKAKFAEAKKELEAKGVQFPIHLDKTVEVTDKVGIQGV
                     SSIKQSIESVLGSDNVVIDIQQLTSDEFDSSGYFAQTAAQKDYDLYHGGWGPDYQDPS
                     TYLDIFNTNSGGVLQNLGLEPGEANDKAKAVGLDVYTQMLEEANKEQDPAKRYEKYAD
                     IQAWLIDSSLVLPSVSRGGTPSLRRTVPFAAAYGLTGTKGVESYKYLKVQDKIVTTDE
                     YAKAREKWLKEKEESNKKAQEELAKHVK"
BASE COUNT        13420 a         8610 c        10531 g        13225 t
ORIGIN      
        1 taataaaagg actaagtgtt tgagatttgt cttcaaacat atgcaattat tcctgaaaga
       61 gtacagttag gagagggtta tgccgattcg aattgataaa aaattgccag ctgttgagat
      121 tttacggaca gagaatatct ttgtcatgga tgatcaacgt gctgcccacc aagatatccg
      181 tcctttgaag attttaattt taaatctcat gccacagaaa atggtcacag agacccagtt
      241 gttgcgccac ttggctaata cacccctaca actggatatt gattttctct atatggagag
      301 ccaccgttct aaaacaactc gttcagagca catggagacc ttctataaaa cttttcctga
      361 agtcaaggat gagtattttg atgggatgat catcacgggt gctccagttg agcatttacc
      421 atttgaggaa gtggactatt gggaggaatt tagacagatg cttgagtggt ctaagactca
      481 tgtctattcg acccttcata tctgttgggg ggctcaggct gggctttatc tgcgctatgg
      541 tgtagaaaaa taccagatgg acagtaagct atcaggtatt tatcctcagg acaccctaaa
      601 agagggtcac cttctattta gaggctttga tgatagctat gtatcccctc attcacggca
      661 cacggagatt tctaaggaag aggtcttaaa caagaccaat ctcgagattt tatcagaagg
      721 acctcaggtt ggggtttcta ttttggccag tcgtgattta cgagaaattt atagttttgg
      781 tcatttggag tatgaccgtg ataccttggc aaaagagtat tttcgagatc gtgatgcagg
      841 tttcgaccca catattccag aaaattactt taaagatgat gatgttaatc aggtaccttg
      901 tctttgttgg tcttcatctg cagccctctt tttcagtaat tgggtaaacc atgcggtcta
      961 tcaggagacg ccttttgatt ggagaaagat agaagatgat gcatctgcat atgggtattt
     1021 ataagaggaa ttatgacata tttagacgct tttaaatcag gtaccttggt tttaccgagt
     1081 gccctgctct tgcattttaa ggaactcttt ccttctagcg acgattttct ggtttggcaa
     1141 tttttctatt tgcaaaatac gacaggctta gaagaaatgt cgccaagcca gattgctgaa
     1201 aggattggca aggaaatttc ggatgtcaac cagtccattt ctaatctgac ggaaagggga
     1261 ctgctccagt atcgtactat cgaattaaat ggcgaaattg aattgttctt tgatgctagt
     1321 ttggccttgg aacgtttgga tgacctgttt ggagcagttc gttcaagttc agaccagcta
     1381 acacctcaaa accagctcaa ggatttggtg gaaaccttcc agcaggagtt gggacgattg
     1441 ttgacgcctt ttgagattga ggatttgacc aagacactaa aggaagatgg aaccagtgct
     1501 gacttgatta aggaggctct tcgtgaagct gttttgaatg gaaaaccaaa ctggaagtac
     1561 attcaggcaa ttttgagaaa ctggcgccat gaaggaatca agagtgtggc tcaaattgag
     1621 gccaagagag cagaaagaga agcaagcaat cctcagttga cacaggtatc tgcagatttc
     1681 agaaatgcca tggatctctg gaaggattaa tccatgcaag taggcttgaa atccgagtaa
     1741 gatttgcaag ctgtgtataa ttgtgataga ataaatagaa aataaattga aaaaagaggt
     1801 atgtgaaatg tcacgtaaac catttatcgc tggtaactgg aaaatgaaca aaaatccaga
     1861 agaagctaaa gcattcgttg aagcagttgc atcaaaactt ccttcatcag atcttgttga
     1921 agcaggtatc gctgctccag ctcttgattt gacaactgtt cttgctgttg caaaaggctc
     1981 gaaccttaaa gttgctgctc aaaactgcta ctttgaaaat gcaggtgctt tcactggtga
     2041 aactagccca caagttttga aagaaatcgg tactgattac gttgttatcg gtcactcaga
     2101 acgccgtgac tacttccatg aaactgacga agatatcaac aaaaaagcaa aagcaatctt
     2161 tgcgaacggt atgcttccaa tcatctgttg tggtgaatca cttgaaactt acgaagctgg
     2221 taaagctgct gaattcgtag gtgctcaagt atctgctgca ttggctggat tgactgctga
     2281 acaagttgct gcctcagtta tcgcttatga gccaatctgg gctatcggta ctggtaaatc
     2341 agcttcacaa gacgatgcac aaaaaatgtg taaagttgtt cgtgacgttg tagctgctga
     2401 ctttggtcaa gaagtcgcag acaaagttcg tgttcaatac ggtggttctg ttaaacctga
     2461 aaatgttgct tcatacatgg cttgcccaga cgttgacggt gcccttgtag gtggtgcgtc
     2521 acttgaagct gaaagcttct tggctttgct tgactttgta aaataatcag taagtagcaa
     2581 aagctaggtg gaacagcatt cagatgtctg ttacattttt tataggagag gaaagattga
     2641 aaataaaaat tggattagca agtatctgtt tactaggctt ggcaactagt catgtcgctg
     2701 caaatgaaac tgaagtagca aaaacttcgc aggatacaac gacagcttca agtagttcag
     2761 agcaaaatca gtcttctaat aaaacgcaaa cgagcgcaga agtacagact aatgctgctg
     2821 cccactggga tggggattat tatgtaaagg atgatggttc taaagctcaa agtgaatgga
     2881 tttttgacaa ctactataag gcttggtttt atattaattc agatggtcgt tactcgcaga
     2941 atgaatggca tggaaattac tacctgaaat caggtggata tatggcccaa aacgagtgga
     3001 tctatgacag taattacaag agttggtttt atctcaagtc agatggggct tatgctcatc
     3061 aagaatggca attgattgga aataagtggt actacttcaa gaagtggggt tacatggcta
     3121 aaagccaatg gcaaggaagt tatttcttga atggtcaagg agctatgatg caaaatgaat
     3181 ggctctatga tccagcctat tctgcttatt tttatctaaa atccgatgga acttatgcta
     3241 accaagagtg gcaaaaagtg ggtggcaaat ggtactattt caagaagtgg ggctatatgg
     3301 ctcggaatga gtggcaaggc aactactatt tgactggaag tggtgccatg gcgactgacg
     3361 aagtgattat ggatggtgct cgctatatct ttgcggcttc tggtgagctc aaagaaaaga
     3421 aagatttgaa tgtcggctgg gttcacagag atggtaaacg ctatttcttt aataatagag
     3481 aagaacaagt gggaaccgaa catgctaaga aaatcattga tattagtgag cacaatggtc
     3541 gtatcaatga ttggaaaaag gttattgatg agaacgaggt ggatggtgtc attgttcgtc
     3601 taggttatag cggtaaagaa gacaaggaat tggcgcataa cattaaggag ttaaaccgtc
     3661 tgggaattcc ttatggtgtc tatctctata cctatgctga aaatgagacc gatgctgaga
     3721 atgacgctaa acagaccatt gaacttataa agaaatacaa tatgaacctg tcttacccta
     3781 tctattatga tgttgagaac tgggaatatg taaataagag caagagagct ccaagtgata
     3841 cagacacttg ggttaaaatc atcaacaagt acatggacac gatgaagcag gcgggttatc
     3901 aaaatgtgta tgtctatagc tatcgtagtt tattacagac gcgtttaaaa cacccagata
     3961 ttttaaaaca tgtaaactgg gtagcggcct atacgaatgc tttagaatgg gaaaaccctt
     4021 attattcagg agaaaaaggt tggcaatata cctcttctga atacatgaaa ggaatccaag
     4081 ggcgcgtaga tgtcagcgtt tggtattaag cgatgatttg aaagagggat gtgatagtag
     4141 caccctcttt ttctttgttt tatgatagtt catcctcgag taaattcaag atcttgctcg
     4201 gaaatgaagc ttatatagta gattgaatat agacaaatac cttgtgattg gtaaaacatt
     4261 ttagaaattc atttaccttt cctaatcgac ttggtttcat cttatttcaa tctattatag
     4321 tattggggaa tttcttcaaa ccacatcagc ttggtcagtt ctacctgcga cctcaaaact
     4381 tgtgctttgg tcaagctggg tttagtttcc tagtttgctg atggatttcc attgactata
     4441 agcatccaac cctctttttg tcttctaaag aattcttaaa ttatcagtct attgcaactt
     4501 ttctcatata agttctttgt cttgctattg gttttcctta gtagtatact aaggtagtaa
     4561 tcattaagaa gtggttacaa aaaataatga atgaggtaaa gaaaatggta gaattgaaaa
     4621 aagaagcagt aaaagacgta acatcattga caaaagcagc gccagtagca ttggcaaaaa
     4681 caaaggaagt cttgaaccaa gctgttgctg atttgtatgt agctcacgtt gctttgcacc
     4741 aagtgcactg gtatatgcat ggtcgtggtt tccttgtatg gcatccaaaa atggatgagt
     4801 acatggaagc tcttgacggt caattggatg aaatcagcga acgcttgatt acactcggtg
     4861 gaagcccatt ctctacattg acagagttcc ttcaaaatag tgaaatcgaa gaagaagctg
     4921 gtgaataccg taatgttgaa gaaagcttgg aacgtgttct tgttatctac cgttacttgt
     4981 cagaactttt ccaaaaaggt ttggatgtca ctgatgaaga aggtgacgat gtgacaaacg
     5041 gtatctttgc aggcgctaaa actgaaacag ataaaacaat ttggatgctt gcagccgaac
     5101 ttggacaagc acctggtttg taagaaacaa ggaaacatag ataaatctgt aggaaatttc
     5161 ttacagattt tttctatttt gtaagctatt ccaaaccagt ctttttttga gttttttgat
     5221 aaaatagtac tatcagtgaa aaggatggaa gcatgactaa gaaaatcgta gctatttggg
     5281 cccaggatga agagggtgtg attggtaagg aaaatcgtct gccttggcat ttgccagcag
     5341 aattgcagca ctttaaagaa acaactctga atcatgctat cttgatgggg cgtgtgacct
     5401 ttgatgggat ggggcgtcgc ttgcttccaa aacgggaaac cctgattttg acgcgtaatc
     5461 cggaagaaaa gatagatggg gttgctactt ttcaggacgt ccagtctgtt cttgactggt
     5521 atcaggatca agaaaagaat ctctacatta tcggtgggaa gcaaattttt caggcttttg
     5581 aaccttacct tgatgaagtg attgtcactc acattcatgc tcgggtggaa ggagatacct
     5641 atttccctaa agaacttgac ttgtctcttt ttgagacagt ttcaagcaaa ttttacgcca
     5701 aagatgagaa gaatccttat gattttacca tccaataccg caagagaaag gaagtctaat
     5761 ggaacggagt ttatttggtc tattcacggc ttttctgtgt ttcatctgct ttctggcagg
     5821 agcacaagct tttcgtaaaa agcgttacgg gctttctatc ctcctttggt taaatgcctt
     5881 taccaatctg gtcaatagta ttcatgcttt ttacatgacc ttattttaga tagaatgaca
     5941 gtatagaatg gaaggaaatc atgtctacaa atagaaaaaa tgatatgatg gtttattgct
     6001 cattttgtgg caaaaaccaa gaagaagtac aaaaaataat tgctggcaac aatgctttta
     6061 tttgtaatga atgcgtggag ttagctcagg aaatcattcg agaagaattg gttgaggaag
     6121 tcttggcaga cttgtctgag gtgccaaaac caattgaact cctccatatc ttgaaccact
     6181 atgtaattgg tcaagatcgt gccaagcgtg ccttggcagt ggcggtttat aaccactaca
     6241 aacgcatcaa tttccacgat acacgcgaag agtcagaaga tgtagatttg cagaagtcaa
     6301 acattttgat gattggccca actggttcag ggaaaacttt ccttgcccag accttggcta
     6361 agagcttgaa tgtacctttt gctattgcgg atgcgacagc tctgacggag gctggttatg
     6421 tgggtgagga cgtggaaaat atcctcctca aactcttgca ggttgctgac tttaacatcg
     6481 aacgtgcaga gcgtggcatt atctatgtgg atgaaattga caagattgcc aagaagagtg
     6541 agaatgtgtc tatcacacgt gatgtttctg gtgaaggggt gcaacaagcc cttctcaaga
     6601 ttattgaggg aactgttgct agcgtgccgc ctcaaggtgg acgcaaacat ccacaacaag
     6661 agatgattca agtggataca aaaaatatcc tcttcatcgt gggtggtgct tttgatggta
     6721 ttgaagaaat tgtcaaacaa cgtctgggtg aaaaagtcat cggatttggt caaaataata
     6781 aggcgattga cgaaaacagc tcatatatgc aagaaatcat cgctgaagac attcaaaaat
     6841 ttggtattat ccctgagttg attggacgct tgcctgtttt tgcggctctt gagcaattga
     6901 ccgttgatga cttggttcgc atcttgaaag agccaagaaa tgccttggtg aaacaatacc
     6961 aaactttgct ttcttatgat gatgttgagt tggaatttga cgacgaagcc cttcaagaga
     7021 ttgctaataa agcaatcgaa cggaagacag gggcgcgtgg acttcgctcc attatcgaag
     7081 aaaccatgct agatgttatg tttgaggtgc cgagtcagga aaatgtgaaa ttggttcgca
     7141 tcactaaaga aactgtcgat ggaacggata aaccgatcct agaaacagcc tagaggtgac
     7201 tatggaactt aatacacaca atgctgaaat cttgctcagt gcagctaata agtcccacta
     7261 tccgcaggat gaactgccag agattgccct agcagggcgt tcaaatgttg gtaaatccag
     7321 ctttatcaac actatgttga accgtaagaa tctcgcccgt acatcaggaa aacctggtaa
     7381 aacccagctc ctgaactttt ttaacattga tgacaagatg cgctttgtgg atgtgcctgg
     7441 ttatggctat gctcgtgttt ctaaaaagga acgtgaaaag tgggggtgca tgattgagga
     7501 gtacttaacg actcgggaaa atctccgtgc ggttgtcagt ctagttgacc ttcgtcatga
     7561 cccgtcagca gatgatgtgc agatgtacga atttctcaag tattatgaga ttccagtcat
     7621 cattgtggcg accaaggcgg acaagattcc tcgtggtaaa tggaacaagc atgaatcagc
     7681 aatcaaaaag aaattaaact ttgacccgag tgacgatttc atcctctttt catctgtcag
     7741 caaggcaggg atggatgagg cttgggatgc aatcttagaa aaattgtgag gaaaagaaaa
     7801 tggcaaaaac aattcataca gataaggccc caaaggctat cggaccctat gttcaaggaa
     7861 aaatcgttgg caaccttttg tttgctagcg gtcaagttcc cctatcccct gaaactgggg
     7921 aaattgtagg agagaatatc caagaacaga cagagcaagt cttgaaaaac atcggtgcta
     7981 ttttggcaga agcaggaaca gactttgacc atgttgtcaa aacaacttgt ttcttgagcg
     8041 atatgaacga ctttgttcct tttaatgagg tttaccaaac ggccttcaaa gaggaattcc
     8101 cagctcgttc agctgtggag gtagctcgtc ttcctcgtga tgtaaaagtc gaaattgaag
     8161 tcatcgcaga gattggataa gctagttgaa gtttggtgtt gccaaacttc ttttgatata
     8221 aggagaaaaa gatgacaaag aaacaacttc acttggtgat tgtgacaggg atgagtggcg
     8281 cagggaaaac tgtagccatt cagtccttcg aggatctagg ttatttcacc attgataata
     8341 tgccgccagc tctcttgcct aagtttttgc agctggttga aattaaggaa gacaatccta
     8401 agttggcctt ggtagtggat atgcgtagcc gttctttctt ttcagagatt caagctgttt
     8461 tggatgagtt ggaaaatcaa gatggtttgg atttcaaaat cctctttttg gatgcggctg
     8521 ataaggaatt ggtcgctcgt tacaaggaaa ccagacggag tcacccacta gcagcagacg
     8581 gtcgtatttt agatggaatc aagttggaac gtgaactctt ggcacctttg aaaaatatga
     8641 gccaaaatgt ggtggatacg actgaactca ctccacgtga gctgcgcaaa acccttgcag
     8701 agcagttttc agaccaagaa caagcccagt ctttccgtat cgaagtcatg tctttcggat
     8761 ttaagtatgg aatcccgatt gatgcggact tggtctttga tgtccgtttc ttgccaaatc
     8821 cctattattt accagaactg agaaaccaaa cgggtgtgga tgaacctgtt tatgattatg
     8881 tcatgaacca tcctgagtca gaagactttt atcaacattt attggccttg attgagccga
     8941 ttctgccaag ttaccaaaag gaaggtaagt ccgttttgac cattgccatg ggatgtacgg
     9001 gtggacaaca ccgtagtgtg gcatttgcta aacgcttggt gcaggactta tccaagaatt
     9061 ggtctgttaa tgaagggcat cgcgacaaag accacagaaa ggaaacggta aaccgttcat
     9121 gagaaaacca aagataacgg tgattggtgg agggactgga attcccgtca ttctaaaaag
     9181 tctgcgggaa aaagatgtgg aaatcgcagc tatcgtgacg gtggcagatg atggtggttc
     9241 ttcaggtgaa ctccgaaaaa atatgcaaca gttgacaccg ccaggtgatt ttcgtaatgt
     9301 ccttgtggcc atgtcggata tgcctaagtt ttatgagaag gtctttcagt atcggttctc
     9361 tgaggatgcc ggagcctttg ctggccatcc attgggaaat ctcatcattg ctggcttgtc
     9421 agaaatgcag ggttcaacct ataatgccat gcagttattg agcaaatttt tccatacaac
     9481 agggaaaatt tatccttcca gtgaccatcc tttgaccctt catgcagtct ttcaggatgg
     9541 gacagaagtg gctggagaga gtcatattgt agaccatcga ggcataattg acaatgtcta
     9601 tgtgaccaat gccctaaacg atgatacgcc tctggccagc cgtcgagtag tgcagaccat
     9661 ccttgaaagt gacatgattg tcctagggcc aggttccctc tttacctcta ttttgcccaa
     9721 tatcgtgatt aaggaaattg ggcgggctct tttggaaacc aaggcagaaa ttgcctatgt
     9781 ctgcaatatc atgacccaac gtggggagac ggaacacttt acagatagcg accacgtgga
     9841 agtcttgcat cgtcaccttg gtcgcccttt tatcgacact gtcttggtga atattgaaaa
     9901 agtgcctcag gaatacatga attccaaccg ttttgatgaa tacttggtgc aagtggaaca
     9961 cgattttgta ggtctttgta agcaagtttc gcgcgtgatt tcatctaact tccttcgtct
    10021 ggaaaatggc ggtgccttcc acgatggaga tttgattgtg gacgagttga tgcgcattat
    10081 acaggtgaaa aaatgagttt cacagtagca gtaaaagaag aaatcctggg ccaacaccat
    10141 ctgagctggc atgaattatc tgccattatc aaaatgtctg gtagcatcgg tctctcgact
    10201 tcgggcttga ctttgtcagt tgtgacagaa aatgccaaac tggcccgtca cctctatgag
    10261 tcctttctcc atttctatga aatcaaatct gaaattcgtc accaccaacg gagcaatctt
    10321 cgcaagaatc gcgtctatac cgtttttaca gatgaaaagg tgcaggattt gttaagtgat
    10381 ttgcacttgg cagactcttt ctttggcctg gaaacaggta ttgatgaggc gattttatcg
    10441 gacgaggaag caggtcgtgc ctatctctgt ggcgctttct tggcaaatgg gagcattcgt
    10501 gaccctgagt caggcaagta ccagttggaa atcagttctg tttatctgga ccacgcgcaa
    10561 ggaattgcct cccttctcca acagttttta ctggatgcca aggtgcttga gcgcaagaag
    10621 ggggctgtga cctatctcca gcgagcagaa gacattatgg acttcttgat agtaatcgga
    10681 gccatgcagg cacgtgatga ttttgagcgg gttaagattt tgcgagaaac ccgtaacgat
    10741 ctcaatcggg ctaataatgc cgagacagct aatatcgctc ggacagtttc tgccagcatg
    10801 aagaccatta acaatatcag taaaataaaa gatatcatgg gcttagaaaa tttgccagta
    10861 gatttgcagg aagtggcgca actgaggatt cagcacccag actactctat ccaacagttg
    10921 gcagatagcc tcagcacccc tctgaccaaa agtggtgtca atcacagact cagaaaaatc
    10981 aataagatag cggatgaatt ataaaaccac gaatcctatg tgactcgtgg ttctttttta
    11041 taaactggta gagtgttttg gttgtacttt ttgttcaggg tcaatgtagt tgatagcgtt
    11101 gttgacagca gttggagctt ctccgaggcc tgtcgcaatc agatcaattt ttccgtcata
    11161 gtagcagcag tcaccgatag catagatacc tgcttggctg gattcctgtt tgctgttgac
    11221 gataatcttg tgacggttga ggtcgagccc ccagttttta aggttaccga cagaagattt
    11281 gaaaccatag ttgacaaaga ggtggtctag atcaatggtt tcagtttcat cagatttgac
    11341 ttttgtgatt tcaagtttat caagtgtttt tccatttcca aggagttggc taggggcgaa
    11401 tggtgtcttg atggttacag atgattcttg caaggcttga acactgtgtt ccaaggcacg
    11461 gaaattatct ctgcggtgaa caagggtagt tggtgcgatt ttttcaaaag ccaaagccca
    11521 atccacagcc gagtctcccc caccaagaat cgtcactttc ttaccagcgt attgctgaat
    11581 gttagaaacg tggtagtgga tattttcata gccctcaacc ccttcaagtt ccagcggacg
    11641 tggtttgaag gcaccgccac ccatagcgat gataactgtt ttagtcaggt gacttccttt
    11701 agaagttgtg atggcaaatt cttcttcttg tttgtcaatc tcaagaaccg tttcattgag
    11761 atgaataggg gtatcaaatc catttagctg ttcaatcaag cggttagtca actcttctcc
    11821 agtcaggttt gggaagcctg gtacgtctag gatttccttt tcagggtaga gaatagcagg
    11881 ttgtccacct agctggggaa gagagtcgat gatttgaacc ttggcttggc gtaggtgggc
    11941 ataaaaggct gcaaaaagcc cgacaggacc accacccaca atggtaatat catagagttg
    12001 agacatagtt tctcctttgt tttttctagt cagtttattt tatcatattt ttgttcaatg
    12061 taaaatggac taggataggg aaaaaaatgc cagaaaatgg tataatagga cgaaagtgtt
    12121 ttgataggga ggatatcagg gatgaatttt caacaattat ccaatctgca atattggacc
    12181 agtttgtttg caagtccatg gacgatagct atcaatctga ttgatatttt gattgttgct
    12241 tatattttat accattttac aaaagctatt gcaggaacca agattatgat tttggtacgt
    12301 ggagttttgg tgtttatttt agctcaaatc cttgcaaata tgattggttt gactacgatt
    12361 tcttggttaa tcaatcaaat tattacttat ggggttattg cggcggttgt tatcttctct
    12421 ccagagattc ggactggttt ggaacgtttg ggaagagcga cagatttctt ttccaatgcc
    12481 cctattagtg ctgaggaaca gatgattcgt gcctttgtta agtctgttga atacatgagt
    12541 cctcgtaaaa tcggggcctt ggttgctatt cagcgtgtac gtaccttgca ggagtatact
    12601 tcgacaggaa ttcccttgga tgctaagatt tctgcagaac ttctcattaa catttttatt
    12661 cccaacactc ccctacatga tggtgcggtg attatcaaag aagaacgtat cgctgtgacg
    12721 tctgcctatc tgcccttgac aaaaaacaca gggatttcca aggaatttgg gaccagacac
    12781 cgggcggcta tcggtttatc agaagtctca gatgccttga cttttgtcgt atcagaggaa
    12841 acgggaggaa tttcgataac ctataatgga aggtttaagc acaacctaac acttgatgaa
    12901 tttgaaacag aattacgtga aatcttactt ccaaaagagg aagtgggtct tagttttaaa
    12961 gaacgattgc taggaggatg gaaacatgaa aaaaaatagt ttatatatca tatcctcact
    13021 cttttttgct tgtgtcttat ttgtctatgc tacggcgacg aattttcaaa acagtaccag
    13081 tgctaggcag gtaaaaacgg aaacctatac taatacagta acaaatgtcc ctattgacat
    13141 acgctataat agtgataagt attttattag cggttttgct tcagaagtat cagtggtctt
    13201 gactggtgca aatcgcctat cgctagctag tgaaatgcaa gaaagtacac gtaaattcaa
    13261 ggttactgct gacctaacag atgctggtgt tggaacgatt gaagttcctt tgagcattga
    13321 agatttaccc aatgggctga ccgctgtggc gactccgcaa aaaattacag tcaagattgg
    13381 taagaaggct cagaaggata aggtaaagat tgtaccagag attgacccta gtcaaattga
    13441 tagtcgggta caaattgaaa atgtcatggt gtcagataaa gaagtgtcga ttacgagtga
    13501 ccaagagaca ttggatagaa ttgataagat tatcgctgtt ttgccaacta gcgaacgtat
    13561 aacaggtaat tacagtggtt cagtaccttt gcaggcaatc gaccgcaatg gtgttgtctt
    13621 accggcagtt atcactccgt ttgatacaat aatgaaggtg actacaaaac cagtagcacc
    13681 aagttcaagc acatcaaatt caagtacaag cagttcatcg gagacatctt cgtcaacgaa
    13741 agcaactagt tcaaaaacga attaaaaata gaaaaggatt ttataacaat gggtaaatat
    13801 tttgggactg atggagtccg tggagaagct aacctagaac taacaccaga attagccttt
    13861 aaactaggac gttttggagg ctatgttctt agtcaacatg aaacggaagc gccgaaagtc
    13921 tttgtaggac gtgacacacg tatttcaggg gaaatgttgg aatcggcctt ggtggcaggt
    13981 ctcctttcag tagggattca cgtatacaaa cttggtgtcc ttgcaacacc agcagtagct
    14041 tacttggttg aaactgaagg agcaagtgcc ggtgtcatga tttctgctag ccacaaccca
    14101 gcccttgata acggaatcaa gttctttggc ggtgatggct tcaaactaga tgatgaaaaa
    14161 gaagcagaaa ttgaagcctt gctagatgct gaggaagaca ctcttcctcg tccaagtgca
    14221 gaaggcttag gaattttggt agattatcca gaaggcttgc gtaagtatga aggatacctt
    14281 gtttcaactg gaactcctct tgatggaatg aaggttgcct tggatacagc taatggagca
    14341 gcttctacca gtgcccgtca aatctttgca gaccttggtg cccaattgac ggttatcggg
    14401 gaaacaccag acggtcttaa catcaacctt aatgttggtt caacacatcc agaagccctt
    14461 caagaagtgg tcaaagaaag tgggtcagct attggtttgg cctttgatgg agacagtgac
    14521 cgcttgattg ctgttgatga gaatggtgac atcgtcgatg gtgacaagat tatgtacatc
    14581 atcggaaaat acctttctga aaaaggacaa ttggctcaaa atacaattgt gacaactgtt
    14641 atgtctaacc ttggtttcca caaggccttg aatcgcgaag gtattaacaa ggcagttact
    14701 gcagttggtg accgctacgt tgttgaagaa atgagaaaat caggttacaa ccttggtggt
    14761 gaacagtctg gtcacgttat cttgatggat tacaatacca caggtgatgg tcaattatca
    14821 gcagttcaat tgactaaaat catgaaggaa actggtaaga gcttatcaga gttggcggca
    14881 gaagtaacga tttatccaca aaaattagtt aatatccgag tggaaaacgt catgaaggaa
    14941 aaggccatgg aagtgccagc tatcaaggcc atcatcgaga agatggaaga agaaatggcg
    15001 gggaacggcc gtatccttgt tcgtccaagt ggaacagagc ccctcttgcg tgttatggca
    15061 gaagcgccta caacagaaga agtggactac tatgttgata ctatcacaga tgtagttcgt
    15121 gctgaaattg ggattgacta aatcctagaa aactagatga aaataaaaaa ttatggtaca
    15181 atagcttata atattgtagc cgatggagaa agacatgaat cttaaacgtg aacaagaatt
    15241 tgttagtcag tatcattttg atgcgcgtaa ttttgaatgg gaaaatgaaa atggagctcc
    15301 tgaaaccaag gtggatgtga atttccaatt gctccaacat gaccaagaaa atcaagtgac
    15361 ttccttgatt gtcatcttga gctttatgat tgtatttgat aagtttgtca ttagtggaac
    15421 aatctcacaa gtaaaccata tcgatggccg tattgtcaat gaaccaagtg aattgaacca
    15481 agaagaggtg gaaactttgg ctcgtccatg tttgaacatg ctcaatcgtt tgacttacga
    15541 agtgactgaa attgcattag acttaccagg aattaatttg gagttctaat atgaaattag
    15601 ctgttttcac agattcctct gcttatctca gtgcagagac cttgcaaaga gaagacttgt
    15661 ttgtcttgga tattcctgtc aatattgatg gtgaggaata tgtcgaaggc atcaatctgt
    15721 ctgctgagga attttaccaa aaaatggctc aggcttctga attgcctaag accagtcaac
    15781 caagtattgc caagttagat gagatcttaa cttcgctcaa agaacaaggc tatacacatg
    15841 ccttggggct tttcctatct tctggaattt caggttttta ccaaaatatc cagtatatgg
    15901 tcgatgacta tgagggctta accattgcgt tcccagacac tttgattaca agtgctccct
    15961 taggcatcat ggttgaaagc gtctttaatt ggcgtgatca gggcgatgat tttgccagca
    16021 ttcaggataa gctagccatt caaatcagcc gtacgtcagc ctttatcatg gtagatgact
    16081 tggatcattt agtgaaaggt ggacgccttt caaatggggc tgccattttg ggcaatttgc
    16141 ttagcattaa gccaatcctt tattttaacg atcaaggtgt gattgaagtt tacgaaaaag
    16201 ttcgtactga aaagaaggcc accaagcgct taattgaaat tatcaaggaa acaacggctt
    16261 caggtcaata ccgggtcatt gtcattcatg ggaatgctcc tgaaaaggct gaagaattgc
    16321 gtcagcattt gcttgatttt ggcttgggtt cggatgtttc acttgctaca tttggtagtg
    16381 tcattggaac gcacctagga gcaggaagta ttgctctggg ttatattcca gtgatttagt
    16441 tggcgcttct aggctagtta agatgccttg tatcttagca attgaacacg gactacctgc
    16501 ctcttgaaaa aagatgtctg tcttgccttt cgtggaaagt cagtgccatt ccctattttt
    16561 cagaggcagc taaggtcctt tgtatcttga taattgaaca cgcctggaac cctgtgtgaa
    16621 aaagatagtt cttccaagaa gtagacactt cttgatcaga attcctattt tcactttgtg
    16681 ttcttacagg ctttgtatct tagacaggag tagagatgag tattcgagta attattgccg
    16741 gttttaaggg aaagatgggc caggctgctt gtcagatggt attgactgat ccagacttgg
    16801 acttggtagc agttttggat ccttttgagt ctgagtcaga atggcagggt attcctgttt
    16861 tcaaggataa ggctgattta gctggttttg aagcggatgt ctgggtagat tttactactc
    16921 cagctgttgc ctacgaaaat acacgttttg ctcttgaaaa tggctttgct ccagtagttg
    16981 gaacgactgg tttcacgagt gaagaaattg cagagctaaa agaattttct cgtgcccaag
    17041 acttgggtgg cctgattgcc cctaactttg ccttgggtgc tgtcttactc atgcaatttg
    17101 cgacgcaggc tgccaaatat ttcccaaatg tggaaattat tgagctccat catgacaaga
    17161 aaaaggatgc tccgagtgga acagccatta aaacagctga gttgatggca gaggttcgag
    17221 agtctattca gcaaggtgca gcagatgagg aagagctgat tgctggtgct cgtggtgctg
    17281 actttgatgg tatgcgcatc cactcagttc gtttgccagg cttggtagct catcaggaag
    17341 tcatctttgg caatcaggga gaagggttga ccctccgtca tgactcctat gatcgcatct
    17401 ccttcatgac aggagtcaat ttgggaatta aagaagttgt caagcgtcat gagcttgtct
    17461 atggattaga acacttatta tgagattaac gcaaatgcct tctgaatttc agaaggcttt
    17521 accagtatta gaaaaaatta aagaagcagg ctttgaggct tattttgttg ggggctctgt
    17581 tcgagatgcc cttctccata gccctataca cgatgtggat attgcgacgt cttcttatcc
    17641 agaagagacc aagcagattt ttccgcgaac agccgatatc ggaatcgagc atggaaccgt
    17701 cttggtctta gatggggatg aggagtatga ggtaacaacc tttcggacag aggatgtcta
    17761 tgtggactat cgcagaccca gtgcggtttc ctttgtgcgc tcgctagaag aagacctcaa
    17821 acgccgtgat ttcacagtca acgcctttgc cttggatgag acaggagaaa tcgttgactt
    17881 gttccatggt ttagaagatt tggaaaagca agtcttgcga gcagttggag tggctagtga
    17941 gcgtttcaat gaagatgctt tacggattat gcgtggtttc cgttttcagg ctagtcttgg
    18001 ttttgcactt gagccagaaa catttaaagc tatgaagacc ttgacgccgc ttttggagaa
    18061 aatttctgta gagcgtacct tcgtcgaatt tgataaactc ttgctggctc cattttggag
    18121 aaggggcttg gcttccatga ttgagagtca agcttatgac tatctccctg atatggcatc
    18181 tagccaggac aagctcaaca gactgtttga tttggagact gattttactt ttgaatcctc
    18241 tgaacaagcc tgggcggctt tactatgggc tttggagatt gaaaatgcgc agtcattttt
    18301 gaaatcttgg aagacctcac gccagtttgc caagcaagtt caggatttgc tgattatttt
    18361 ggctctgcgt gaaaatggag aattgagcaa gcgagattgt tatcgctttg acatagattt
    18421 gcttttacag gctgaaaatc ttcgtcaagc tcaaggaaaa gaagtcaacc cacaagccat
    18481 cacagaaaaa taccaaagct tgactatcca tgacaagaaa gagattcaga ttaatggcgg
    18541 tattttgatc aaggaatatg gctatcagcc aggcccagac ttgggagaga ttttaacaga
    18601 gattgagttt gccattgtcg atggagaatt ggagaataat cgtgaagcca tccatgctta
    18661 cctgagggag aaaaaatgag tgattttatc gttgaaaaac taagtaaatc cgttggtgac
    18721 aagaccgttt ttagggatat ttcctttatt atccatgact tagacagaat tggtttaatc
    18781 ggtgtcaatg ggactggcaa gaccaccctt ttggacgtcc tttctggtgt ttctggattt
    18841 gatggggatg tcagtccttt ttcagctaaa aatgattacc agattggtta cttgactcag
    18901 gatcctgatt ttgatgatag aaagacagtt ttggatacgg ttctatctag tgaactcaag
    18961 gaaatccagc tcattcgtga gtatgaattg attatgctcg actatagtga ggacaagcag
    19021 gcgcgtttgg aacgtgtcat ggcagagatg gactctctcc aagcttggga aatcgaaagt
    19081 caggtcaaga ccgttcttag caaattgggc attcaagact tatctactcc tgttggggaa
    19141 ttgtcaggtg gtctgagaag acgggtacag ttggcacaag tcttacttgg caaccacgac
    19201 ctcttgcttt tggatgagcc gaccaaccat ctggatattg cgattattga gtggctgacc
    19261 ctctttttga aaaattctaa gaagaccgtc ctttttatca ctcacgatcg ttatttctta
    19321 gacgctttgt caacacggat tttcgagttg gatcgtgcag gcttgaccga gtaccaggga
    19381 aattaccagg actatgttcg cctaaaggcg gaacaggatg agcgcgacgc ggctcttctt
    19441 cacaaaaaag aacaactcta caaacaagaa ttggcctgga tgcgcagaca accgcaggcg
    19501 cgtgcgacca agcagcaggc ccgtatcaat cgattccatg atctgaaaaa gaaagtttca
    19561 ggcagtagtg ctgagacaga cttgaccatg aactttgaaa ccagtcggat tgggaagaaa
    19621 gtcatcgagt ttcaggctgt ttcctttgcc tatgaaaaca agcctatttt gcaagatttt
    19681 aatctcttgg tgcaggccaa agaccgtatc ggaatcgttg gggacaatgg tgttggaaaa
    19741 tcaaccctgc tcaacttgat tgcaggaagt cttgagccaa ctaaaggaca agttgtgatt
    19801 ggggaaactg ttcgcatcgc ctatttctct cagcaaattg agggtttgga tgaaagcaag
    19861 agagttatca attacctgca ggaagtggca gaggaggtca agaccagtgg cggttctacg
    19921 acttcgattg cagagttgct ggagcagttc cttttcccac gttcgacgca tggaaccttg
    19981 attgagaaat tgtcaggggg tgagaaaaaa cgtctttatc tcctcaaact gcttttggaa
    20041 aaaccaaatg ttcttctttt agacgagcca accaatgacc tagatattgc aactttgaca
    20101 gtcttagaga atttcttgca aggttttgca ggtcccgttt taacagtcag tcacgaccgc
    20161 tatttcttgg ataaggtagc gaccaagatt ctcgcttttg aggatggcaa gattcgtcct
    20221 ttctttggtc attacaccga ctatcttgat gaaaaagctt ttgaaacaga tatggccaat
    20281 caagtgcaaa aggccgaaaa ggaaaaagtg gtcaaggttc gagaagacaa gaaacgcatg
    20341 acctaccaag aaaagcagga gtgggcaagt attgaaggtg atattgaaac cttggaaaaa
    20401 cgtatcgctg ctattgaaga ggaaatgcag gctaacggct ctgactttgg taagctggct
    20461 actctccaaa aagaattgga tgagaaaaat gaagcactcc ttgaaaaata cgaacgctat
    20521 gagtatctca gtgaatttga tagttaagac tattcgaaaa tcaaagtgca aactatgtcg
    20581 gcttcacatt gccgactcaa gtacagcctg tgaccggctt cctaatttgc actttgattt
    20641 ttattgagta taaaagaata gaaaaatccc gtctcatgaa aggatttctc tattcttttt
    20701 tggtttcttg atgaaagata ttttgccagg tttcgtggcg acgccagata ctggtatgga
    20761 tgataccatc taactcatag cagatgagtt tggttttttg actgatggaa gtaatttgaa
    20821 tgtccttgat agcagaattt aactcttttt tggtcttata agcctcttta tccatctgct
    20881 ctccatcttg gcgaatatag acaaaatcat cagtcaagag ttcttctagt tggtttcctt
    20941 ggtcaatcaa ttgttcacgc ataagcaatt ttttatagac attgtctaaa atttcatcct
    21001 cagggatagg tgctggttcg atatggacat cggtatcaaa gacgccaaaa cgctcctcca
    21061 gcatagactc gacctgatcc gcgatttcat ggctttcaaa aacagacaag tcaggattca
    21121 tctctagtgt aatatccagg tagatgttgc taccgtaggt gcgacctctt tgcgatttga
    21181 ccttgctgat tttgggaatt tccatgatag ccttttggta gtcctcgagc aggcggtcgt
    21241 caaagccatc tgaaagacta aaggaagact cgatgaagat atcataggca gtcttcaaga
    21301 taaagaaagt gatgatgata gcaaccagtt tatccacaat cggataattg aaactactag
    21361 ctaggatggc aatggcggtt ccaagtgagg taacagcgtc agaaagattg tccttagcag
    21421 ctgccttcag cgccttggag ttggatttct tactgaggcg agtattgtag agatagacca
    21481 caaacataat cgctgcagaa atgattccta gagttgcacc aagaggatca atgaccgttt
    21541 cttcccgact gagaatcttt tgaatggtat ctcttagaac atcgaaaccg acatagaaca
    21601 tgatgataga agtgatcaag cttgccaaat cttcaatctt ccaatgacca aaacggtggt
    21661 cacggtctgc aggctggcgc gccatccgaa tcccgattaa gagggccaca tttccaatga
    21721 tgtccgatac gttattaaaa ccatcggcca ccaaactgga tgaatgaagg agatgaccag
    21781 ctgctaattt ggctgcagac aagatcaaat aggtcgaaat actgataatg gctccacgct
    21841 cagctaactt gagatttgag atagattgct tcattcaact tcctttctag tcacatagca
    21901 ttctaccatt atactggttt tcaaggaaaa attcaaataa ggcagttttt actctttgaa
    21961 aactccccaa aatttggtat aatggtacta ctcaagggag tagctggcag aaacctgtga
    22021 tagtgtcgtc attccgaatt ttatgctgaa aagtatgctt tccggcccta tcttaaacag
    22081 cgagacttgt tatgattaac aggtctcttt ttgtttgtcg tgaaaagata tgacagaaag
    22141 agagtctgtt ttgcacacaa tgtcaaggag gagacacatg tcaaaagaac aaaaacgcca
    22201 agcgttttat actcagagcc ctgaagaggt cttgcaggct gtggatgcga ccgagcaagg
    22261 tttgtcatca agtgaggcgg aaaagcgcct tgccgaattt gggcacaatg aactcgaaga
    22321 aggcgagaaa cgatcaatct tggtcaaatt catcgagcaa tttaaggatt tgatgattat
    22381 catcctagtt gcggcagcaa tcttgtcagt cgtgacttct ggtggggaag atatcgcaga
    22441 tgccattatc atcctagctg tggttatcat caacgctgcc tttggtgttt accaagaagg
    22501 aaaagctgaa gaagctattg aagccctcaa atccatgtct agtccagttg cccgcgttct
    22561 tcgtgatgga cacatggcag agattgactc taaagaattg gtaccaggcg atatcgttgc
    22621 ccttgaagca ggtgacgtgg taccagcgga cctacgtttg ctagaagcca actctcttaa
    22681 aattgaagaa gcagccttga cgggtgaatc tgtaccagtc gaaaaagact tgtcagtcga
    22741 gcttgcgaca gatgctggta ttggtgaccg tgtcaacatg gccttccaaa actcaaacgt
    22801 aacctatggt cgtgggatgg gtgttgttgt caatacaggt atgtacactg aagttggtca
    22861 tattgctggt atgcttcaag atgcggatga gactgataca ccactcaaac aaaacttgaa
    22921 caacctttct aaggtcttga cctatgctat cttggtcatt gcccttgtta cttttgtagt
    22981 gggtgtcttc attcaaggga aaaatccact tggtgagttg ttgacttctg ttgcccttgc
    23041 tgttgcagcc attccagaag gacttcctgc tatcgttacc atcgttcttt ctcttggtac
    23101 tcaagttttg gccaaacgac attccatcgt tcgtaagttg ccagcagttg aaacacttgg
    23161 ttcaactgaa atcatcgctt ctgataagac tggtacgctg actatgaaca agatgacagt
    23221 cgaaaaagtc ttctacgatg cggttctaca tgactcagct gatgatattg aactaggtct
    23281 tgaaatgcca ctacttcgtt cagttgtctt ggccaatgat acgaaaatcg atgtggaagg
    23341 taacttgatt ggtgacccaa ccgaaacagc ctttatccaa tatgccttgg acaagggcta
    23401 tgatgtcaaa ggtttcttag agaaatatcc tcgtgtagct gaattgccat ttgactctga
    23461 ccgtaagctc atgtcaacag ttcacccatt gccagatggt cgtttccttg tagcagtcaa
    23521 gggtgcgcca gaccaactct taaaacgttg tcttcttcgt gataaggctg gggatattgc
    23581 tccgattgat gagaaggtta caaatctcat tcatacaaac aattctgaaa tggctcatca
    23641 agccttgcgt gtccttgcag gtgcttataa gattatcgat agtattccag aaaatctcac
    23701 ttctgaagag cttgaaaatg atttaatttt tactggtttg attgggatga ttgatcctga
    23761 acgtcctgaa gctgctgagg ctgttcgtgt ggctaaggaa gctggaattc gtccaattat
    23821 gattacaggt gaccaccaag acaccgcaga agctattgcc aaacgtttgg gaatcattga
    23881 cgcaaacgat acagaagacc acgttttaac tggtgctgag ctcaacgaat tatctgatga
    23941 agactttgaa aaagttgttg gtcaatactc tgtttatgcc cgtgtgtctc cagagcacaa
    24001 ggttcgtatc gtcaaggctt ggcaaaaaca aggtaaggtc gttgccatga caggtgacgg
    24061 tgtcaatgat gccccagctc tgaaaacagc agacatcggt atcggtatgg gaatcactgg
    24121 tacagaggtt tctaaggggg cttctgatat gattcttgca gatgataact ttgcgactat
    24181 tatcgtcgca gtggaagaag gacgtaaggt cttctcaaat attcaaaaga ctattcagta
    24241 cctactttct gccaatactg ctgaagtatt aaccatcttc ctatcaacct tgtttggttg
    24301 ggacgtcttg cagccagttc atcttttgtg gattaacttg gtaacggata ccttcccagc
    24361 aattgcacta ggtgttgaac ctgcggaacc tggtgtcatg aatcataaac cacgtggacg
    24421 caaggcaagc ttcttctcag gtggtgtttt gagttctatc atttatcaag gtgtactcca
    24481 agcagctctt gttatgagtg tttatggcct tgcgattgct tacccagttc atgtgggtga
    24541 caatcatgct attcatgcag atgccctaac gatggccttt gcaacccttg gtttgattca
    24601 gctcttccat gcctacaatg tcaagtctgt ctaccaatcc atcttgacag ttggcccatt
    24661 caagtctaag acctttaact ggtccatctt ggtatccttt atccttctca tggcaacaat
    24721 cgttgtagaa ccgcttgaag gaatcttcca cgtaaccaaa ctagacttgt cacaatgggg
    24781 aattgttatg gctggaagct tctcaatgat tatcatcgtc gaaatcgtta agtttattca
    24841 acgcaaactc ggttttgaca agaatgcgat ttaagggagg tttcggatgt cagcatatca
    24901 attaccgacc gtatggcagg atgaagctag taatcaagga gcttttacgg ggctaaacag
    24961 accaacagta ggtgcccgtt tcgaacaaaa cttgccaaaa ggagaacaag cttttcagct
    25021 ttattcactg ggaacaccaa atggtgtgaa ggttactatc ttattggaag aattactaga
    25081 agctggtttt aaggaagcgg cttacgactt gtataagatt gctatcatgg atggggatca
    25141 attcggatca gactttgtga agctcaatcc aaattccaag attccagcct tattggacca
    25201 gtcaggtact gaagatgtaa gagtctttga gtctgctcat attcttcttt accttgctga
    25261 gaaatttgga gcctttttac caagtaatcc tgtggaaaag gtagaagttt tgaattggct
    25321 attctggcaa gcaggtgcag caccttttct aggtggggga tttggacatt tcttcaatta
    25381 tgctcctgaa aaattggaat atcctattaa ccgttttaca atggaagtga aacgccagtt
    25441 ggatttattg gataaggaat tggctcagaa accttatatt gcaggcaatg actatacgat
    25501 tgcagatatt gctatctggt cttggtatgg acagttagtt caaggaaatc tttaccaagg
    25561 ttctgcaaaa ttcttggatg cctcaagtta tcaaaatcta gtaaaatggg cagaaaaaat
    25621 tgccaatcgt ccagctgtta agcgtggctt ggaagtaact tatacagaaa ttaaatagca
    25681 aaaaagtcat cttcggatgg ctttttgcta cagttgaggg aaagggtttg cagttatcgg
    25741 cttttgtgct ataattgcta taatatagta aaaagcaaga ggagtgtgag gaagtggaaa
    25801 agaaaattgt gaaggatatc ttatttttat ctcaagtgtc tcagccggca agtcaggagg
    25861 acctttatct tgccagagat ttgcaggata cactcttagc aaatcatgat acctgtgttg
    25921 gtctagctgc caatatgatt ggggtgcaga agcgcgtgat tatctttaat cttggcttgg
    25981 ttcccgtggt catgtttaac ccagtgcttc tgtcctttga aggatcctat gaggcagaag
    26041 aaggctgttt gtccttggta ggcgtgagat caactaagcg ttatgaaacc ataagggttg
    26101 cctatcgtga cagcaagtgg caggaacaga ccattacctt gacaggcttc ccagctcaga
    26161 tttgccagca tgagctggat cacttggaag gacgaatcat ttaggaggaa agcaaatgaa
    26221 acgaatagtc tttgaactta tttttatcgc aacgacctgg tatatctttt taccgcccct
    26281 taacctgacc agctgggaat ttctcttctt cctctgtggg catttgttag ttgtggcaat
    26341 cttatttggc tttggcaagg ggataaacct tgtcaaaacg gttcatgtgc gccacggtaa
    26401 ggcggaagct gccttaaatc ttgagggttt caaaatcaat cggttaggga aaattctgtt
    26461 agcttcgatt ggaggaattc ttctcttggc agctttggtt tccttggtaa cttccagcat
    26521 gtttcaggct aaaaattatg ccaatgtagt cacggttacg gaaaaagact ttactgaatt
    26581 tcctaggagt gacaccagta aggttcctat cctagataga agtactgctg aaaaaattgg
    26641 agaccgctac ttgggttccc taaccgataa ggtgtcgcaa tacgtagcgg cagataccta
    26701 tacccaattg acaattgatg ggaaacctta tcgggtcaca ccactagaat atgcagaccc
    26761 tatcaaatgg tttaacaatc aagccaaggg aatcggtgag tatatcaagg tggacatggt
    26821 aactggaaat gcggatttgg tggacttgaa gacaccaatc aagtattcag actcggagta
    26881 ttttaaccgt gatgtcaaac gtcacctgcg cttgaagtac ccgaccaaaa tctttaaaac
    26941 tccatctttt gaggtggacg atgagggcaa tcctttctat gtagcaacgg tttaccaaaa
    27001 gcaatttgga cttgctgttc ctcgtcctgc ttcagtcatt atcttggatg ctacaaatgg
    27061 agaaaccaag gaatacagct tatcagatgt tccagaatgg gtggacagga tctatccagc
    27121 agaggaaacc attgagcaaa tcaactacaa cggcaagtac aaggacggtt tcttgaatgc
    27181 catgatttcc aagaaaaacg tgacccagac taccaagggc tataattact tgtctatcgg
    27241 taatgacatc tatctctaca caggtgtgac gtcggctaat gcggatgaga gtaatcttgg
    27301 tttcatcctt gaaaatatgc gaacaggaga aatcactaag tatagcttgg cttctgcgac
    27361 agaagaatca gcccgtgaat cagcagaagg tgctgttcag gagaaatcct acaaagcaac
    27421 cttcccaatc ctcatcaacc tcaatgacaa gcctctctac atcatgggct tgaaggacaa
    27481 tgctggcttg gtcaaagagt acgccctggt agacgcagtc gagtaccaaa atgttatcgt
    27541 tgctactaca gtggaagaga tgctcagcaa gtatgccaat aaaaacgacc ttgaaattga
    27601 caatgcaacg acagaaagca tcaatggagt agtagcagac ctcaaatcag ctgttatcaa
    27661 gggagacact gtctacttct ttaaagttga tggcaagata tacaaggtca aggcttcagt
    27721 atccgatgac cttccttacc ttgaaaatgg taaaaccttc gaaggtcaag taggaaaaga
    27781 caattatctc aagaccttta agctacggta aaaataggtt tttttcagaa agtatatgtt
    27841 ataataaggt aaattaagcc taatggagga caaagaaatg ggtttttatt tgatggttgc
    27901 ttcaatgcta ctcggactgc tcgctttgaa gataggtttt tctcagttca aggagaaaaa
    27961 agataaattc ttatctatct taacaagttt agctggcaca gcccttgttc tcgtggctgt
    28021 ctggttagga tggcccaaat aaatagaaaa acctcggtca aaccgaggtt tttcaaatga
    28081 atttcttggt tgtggctaaa aaatatgcta cactatcaat atgaaaattt taatcccaac
    28141 agcaaaagaa atgaacacag acttgccaag tatcgaggca atttctttaa aaccagaaag
    28201 tcaggccgtg cttgatgcct tggctctcta ttctgccagt caattggaga gtttctacaa
    28261 ggtatcagct gagaaagcgg cggaagaatt tcaaaatatc caagctttga aaaggcaaac
    28321 tactcaacac tatccagcct tgaaactttt tgatgggctt atgtaccgca acattaagag
    28381 agataagctg accgaggcgg aacaagatta tcttgaaaat catgtcttca ttacctcggc
    28441 tttgtacggt gttgttccag ccttgtcacc catggctcct caccgtttgg attttttgat
    28501 gaaattaaaa gttgctggta agactttgaa gagccattgg aaggcagtct atgatgaagc
    28561 tctgaagaag gaagaagtga ttttctctct cttgtcatca gagtttgaga ctgtattttc
    28621 taaggaaatc agagcaaaga tggtgacctt caaattcatg gaggatagag gcggtcagct
    28681 gaagattcac tcaactatct ccaagaaagc gcgcggggcc tttctaacag ctttaataga
    28741 aaatcaagta caaactgtgg gggaagcacg tcgcttgaac tttgctggat ttgtttaccg
    28801 agaagatttg tcacaaccac agggattggt ttttgtaaag gaagtataga aatcagatat
    28861 tttgtatttg gcaatatgta tcatcagcta ttttagttta taaatgagaa aaaagcgaac
    28921 aagctagtct tctgagcgtc agaaaagtag tcttgttcgc ttttattatg ttatactctt
    28981 cgaaaatctc ttcaagccat gtcagtgtcg ccttaccgta tatatgttac tgacttcgtc
    29041 agttctatct acaacctcaa agcagtgctt tgagtagctt gcggctagct tcctagtttg
    29101 ctttttgatt tttattgagt gttagttact tgatagcttc gaccaagtct tgagcttgtt
    29161 tttcaagtga gtttaggact gtttcttcaa gaaccaattt tccgtctgcc caggcagagt
    29221 cattaacacg tgcagcagtg aaatcaccaa cgccttgtgt acggataaat ggcaagaggt
    29281 ctttgtagat agcgaagagt tgatcgtgac ctgcattggc tacagatgat actgtgacga
    29341 acttgtcttg aagggcagaa gcgccgcgtg tatcagacaa gtcaagggca cgagatagcc
    29401 agtcaagcaa gtttttcact gttccaggga tagagaagtt gtagactgga gagaagatcc
    29461 agatggcatc cgcagcaagg acagcttcac gagctgccgc tacagctgga tgagttggaa
    29521 cttccaaatc ttggctgaag agaggaaggg ctgaataatc aaggtagcta acttctgctt
    29581 taccagcaag tgctttctca gcttcgaggg ccatttggtg gttgaaagaa ccttggcgta
    29641 gtgatccgac gataaataat acttttttag acatgatgtg tctccttttt cttaaattta
    29701 cgagaatttt ctcgttgtta taaaatatat tacaacaaag caaaacaact tgcaagaaat
    29761 ttgctcagag tttatttgaa aattataaaa ttgctgaaac tctagtcact tgtgactgaa
    29821 aaagtaagaa ctgtttcact ttcaaaatcg gttttgtttg aagggagaaa tagaattggt
    29881 tgctcaaagc acagctttga ggttgcagat aaagctgacg tggtttgaag agattttcga
    29941 agagtattct tatattcact tgtaataaag cttaatagat ggtaaaatag acgagtgaaa
    30001 aaaagacaaa acaaagcaaa aaataatcta ctgtggcagt atggtctagg gatgacgatt
    30061 ttgtttgtgg ttatcagtgc ttcctttctg tatatggttt ctcttagcat gaaaccctat
    30121 caaacagcta aaagtgaagg agaaaaatta gctcagcagt atgcaggatt agagcaggcc
    30181 gatcaggttg atttatacaa tggcttggaa tcttattaca gcgttcttgg tcgtaataaa
    30241 cagcaagaag cacttgctgt tctgattgga aaagatgatc ataagattta cgtttatcag
    30301 ctaaatcagg gtgtttcaca agaaaaagca gaaacggttt ctaaggaaaa gggagctggc
    30361 gaaattgaca agattatctt tggtcgttat caagataagc caatctggga agtcaagtca
    30421 ggatctgatt tttatctagt agattttgaa acaggagcat tggtcaacaa ggagggccta
    30481 tgaaattatc caaccgtgtt ttagaaatgg aagaaagtgt gactttggct gctggagccc
    30541 gtgccaaagc actgaaggct gagggcagag atattttgtc tctaaccttg ggtgagccag
    30601 atttcactac tcccaaaaat atccaagatg ccgccattgc atcgattcga gatgggcgtg
    30661 cttcttttta tacagtaacc tcaggtctgc cagagcttaa ggcggtggtc aatagctact
    30721 ttgagcgctt ttacggctat tctgtagcgc caaatcaagt gacagtcgct gcgggagcca
    30781 aatattctct ctataccttc tttatggctg tggtcaatcc aggtgatgaa gtcatcatcc
    30841 caaccccata ctgggttagc tatggagatc aggtcaagat ggcagaaggc gttcccgtct
    30901 ttgtttctgc taaggaagac aatcacttta aggtgaccgt agagcagtta gaagcagctc
    30961 gcactgacaa gaccaaggtt ttggtgctga attcgccatc taatcccaca ggtatgattt
    31021 acacccgtga ggaactcttg gcaattggaa actgggctgt agaaaatgat attctcatcc
    31081 tagcagatga tatctatggc cgcttggttt ataatggtca tgagttcaca ccgatttcta
    31141 gcctatcgga agcgattcgc aagcaaacag tggtcatcaa tggtgtgtct aaaacctatg
    31201 ccatgactgg ttggcggatt ggttatgccg ttggagaagc agacattatc gctgccatgt
    31261 ccaagattgc aggtcaaaca acttcgaatc cgtcagcagt agcccaatat gcagcagttg
    31321 aggctctatc aggtgagcaa gatactgtag aaagcatgcg tcaggccttt gagaaacgtc
    31381 ttaataccat ctatcccctc cttgcagagg tgccaggatt tgaagtggtc aaaccacaag
    31441 gggctttcta tctattccca aatgtcaaaa aggccatgga gatgaaaggc tacacggatg
    31501 tgacagactt tacaactgct atattagaag aagccgaagt ggccttggta acaggagctg
    31561 gatttggagc accagaaaat gtccgcctca gctatgcgac agacctagac acgcttaaag
    31621 aagcagtcga acgcttgaaa gcatttatgg gtagtgagaa tgattgatca ttttgagatt
    31681 aaggtaaagg atttacaaat ttcagaagga ttttatagga gttttctcgc tcctttggac
    31741 tataaattgg cttttaagac tagttctcta attagttttc tatccccgaa cagccctcat
    31801 cctggtggtg atttttggct gactcaggga acacaagatc ctgttcattt tgctttttta
    31861 gcagaaaata aggaagaagt tcaggcttgt tatgaggctg gcatagaggc aggtgggcga
    31921 gacaatgggg ttcctggtta tcgaagtgag catccgattt actatgctgc ttttatgatt
    31981 gacctggatg ggaacaatat agaggtggtt tgccataaag aataaaaaag aaaaggaaaa
    32041 ataatgacaa aacgtgtaac gattattgac gtaaaagact atgttggtca ggaagtgacg
    32101 attggcgctt gggttgccaa caaatcagga aaaggaaaaa tcgctttctt acaattgcgt
    32161 gatggaacag ccttctttca aggtgtggct tttaaaccaa actttgtcga aaaatttggt
    32221 gaagaagtgg gacttgagaa gtttgatgtt atcaaacgct tgagccaaga aacgtctgtt
    32281 tatgtgacag gtattgtcaa agaggacgaa cgttctaaat ttggctatga gttggacatc
    32341 acagacatcg aagtgatcgg tgaatctcaa gactacccaa tcacaccaaa agaacacgga
    32401 acagactttt tgatggataa ccgtcacttg tggctacgct ctcgtaagca agtagctgtg
    32461 ttgcaaatcc gtaacgctat tatctatgca acttatgagt tctttgacaa gaacggtttt
    32521 atgaagtttg acagcccaat tctttcagga aatgcggcag aagattctac agaactcttt
    32581 gaaactgact acttcggaac gccagcctac ttgagccaat caggtcagct ttacctagaa
    32641 gcaggggcta tggctcttgg tcgtgtcttt gactttggtc cagttttccg tgctgaaaaa
    32701 tcaaaaacac gccgtcactt gactgagttc tggatgatgg atgctgagta ctcatacttg
    32761 acacatgatg agtcgcttga cttgcaagaa gcttatgtga aagctcttct acaaggtgtt
    32821 cttgaccgcg cgcctcaagc cttggaaacc ttggaacgtg atacagaact cttgaaacgc
    32881 tacattgcag agccattcaa acgtatcact tacgatcaag ccattgacct cttgcaagag
    32941 catgaaaatg atgaagatgc tgactacgag catcttgagc atggtgatga ctttgggtca
    33001 ccacacgaaa cttggatttc aaaccacttt ggtgtgccaa catttgtcat gaactatcca
    33061 gcagccatca aggccttcta catgaaacca gttcctggaa atccagagcg cgtgctttgt
    33121 gcagacttgc ttgctccaga aggctatgga gaaattatcg gtgggtctat gcgtgaggaa
    33181 gattacgatg cccttgtcgc taagatggat gaacttggca tggatcgtac agaatatgaa
    33241 ttctaccttg accttcgtaa atacggtaca gttccacacg gaggatttgg tatcggtatc
    33301 gaacgtatgg taaccttcgc agcaggaaca aaacatatcc gtgaagctat tccattccca
    33361 cgtatgttgc accgtatcaa accataagaa caggaaaacg cccatgtggc gttttttcga
    33421 attctagtgt ttttacttgc gtaaaaaaat tttttctagt ataatagttt attgtgagcg
    33481 aacctcactt accccttgca aagtcttggg gtcattagac caaaaggagg aacatatcaa
    33541 tggctaaata cgaaattctt tatatcattc gtccaaacat tgaagaagaa gctaaaaacg
    33601 ctttggtagc acgttttgac tctattttga ctgacaacgg tgcaactgtt gttgaatcaa
    33661 aaacttggga aaaacgtcgt cttgcatacg aaatccaaga tttccgtgaa ggactttacc
    33721 acatcgttaa cgttgaagca aatgacgatg cagctcttaa agagtttgac cgtctttcca
    33781 aaatcaacgc tgacattctt cgtcacatga tcgtcaaaat tgacgcgtaa gaaggtaaac
    33841 tatgattaac aatgttgtac ttgtagggcg tatgacacgt gacgctgagt tgcgttatac
    33901 cccatcaaat gtagcagttg cgacttttac tcttgcagta aaccgtatat ttaagagtca
    33961 aaatggtgaa cgtgaggctg attttattaa tgtcgttatg tggcgccaac aggctgaaaa
    34021 tcttgctaac tgggctaaaa aaggctcact tatcggggtg acaggtcgta tccagactcg
    34081 tagttacgat aaccagcaag gacaacgtgt ctacgtgaca gaggtcgtgg ctgagaattt
    34141 ccaaatgttg gaaagccgta gtgtgcgtga gggtcacaca ggtggagctt actctgcacc
    34201 aactgcaaac tattcagcac ctacaaattc agtaccagac ttttcacgta atgaaaatcc
    34261 atttggagca acaaacccat tggatatttc agatgatgat ttaccattct aatggacaac
    34321 taaacttata aaggagaaaa aacatggctc aacaacgtcg tggcggattc aaacgccgta
    34381 aaaaagttga ttacatcgca gcaaacaaaa ttgaatatgt tgattacaaa gatactgagc
    34441 ttcttagccg tttcgtttca gaacgtggga aaatccttcc tcgtcgtgta acaggaactt
    34501 cagctaaaaa ccaacgtaaa gtaacaacag ctatcaaacg cgctcgcgta atggctttga
    34561 tgcctttcgt aaacgaagat taaagaaaag acccggttgg gtctcttttt catgagcttg
    34621 aaaacaagaa agcgaattaa gaccccaacg ggtctttttt cacaagcttt gaaatgagag
    34681 agcgagtcag gctcagtggc tcccttttta cgagtttgaa aacaagagaa cacattaaaa
    34741 gcacgatgga gctttttcat aaatatgtct tttttctcat ccaagaaatt aaaataaaaa
    34801 tgataaactt tttgaaaata ggggatttgc ctactttatg atataatgga aagagttaga
    34861 ttgaaagagg tagtgagatg gacgcgaaat taagatacaa ggcaaagaag atcaagattg
    34921 tcttttttga tattgatgat acattgcgga attcaaagac agggtttatt ccaactacaa
    34981 ttcccactgt ttttaaacag ttgcgtgaaa aaggaatttt aacaggaatc gcctctggac
    35041 gtggcatttt tggtgttgtt ccagagattc gtgatctcaa gcctgacttt tttgtaactt
    35101 tgaatggggc ttatatcgaa gataaaaaag gtcaggtcat ttatcagcat cagattgaaa
    35161 agtcatatgt tgaggagtat atctcttggg ctaagcaaga aggaattgag tatggcttgg
    35221 ttgggagtca tgatgccaag ttgtcgactc gcaccgatat gatgagtgaa gctatcaatc
    35281 caatttatcc cgacttagat gtagatcccg atttccatga aaaagaagat atctatcaga
    35341 tgtggacttt tgaagataag ggagatgact tgcacttgcc tgacagtctc tcagacaaac
    35401 ttcgcatggt tcgttggcat caacattcgt ctgatattgt gccgatttca ggctccaaag
    35461 cgacgggggt ggaaaaggtt gtggaacacc ttggcttgaa accagagaag gtcatggttt
    35521 ttggagatgg tctcaatgac ttggaactct ttgattatgc tggaatcagc gttgccatgg
    35581 gaatttctca tgataaaatc aaagaaaaag cagattatat tacaaaaaca ttagaagaag
    35641 atggcatttt tgatgcctta gaagtatttg gtatggtaga aaaagaattg cattttccac
    35701 aagtagacat tgaaacagta gaaggtcctc ttgcgactat taagaccaat cacggagact
    35761 tacgtatcaa gcttttccct gaacatgctc ctaaaacagt ggctaacttt gtatctcttt
    35821 caaaagatgg ctactatgat ggtgtcattt tccaccgtat tatcaaggac tttatgatcc
    35881 aaggtggaga cccaactgga actggtatgg gtggcgagtc aatctacggc gaatcttttg
    35941 aggatgaatt ctcagaagag ctttacaata tccgtggtgc tctttccatg gcaaatgctg
    36001 gtccaaatac caacggcagc cagttcttta tcgtgcaaaa ccaacaccta ccttattcta
    36061 agaaagaaat tactcgtggt ggttggccag aaccgattgc agaaatctat gccaatcaag
    36121 gtgggacacc tcacctagac cgccgtcaca cggtttttgg tcagttagct gatgaagcat
    36181 cttacgctgt cttggatgtc attgctgctg ttgagacagg agctatggac aagccagttg
    36241 aagatgttgt aattgaaact attgaaatcg aggactaaga tgaaaatcgg tgataagcta
    36301 aaggggcgta ttacagggat tcagccctac ggtgcctttg ttgagttaga gacgggtgat
    36361 acggggctga ttcacatttc agaaattcgg acaggattta ttgaaaatat tcatgaaacc
    36421 ttgaaagtcg atgaagaggt tcaagttcag gtagtggatt tagatgaatt tacggggaaa
    36481 gccagtcttt ctatccgcac tttggaggaa gaaaagtacc agtttcctag acggagacgc
    36541 ttttcaagcg accgctttaa ctacggtttt gctccctttc gacgaatgct accaatctgg
    36601 actggagaag ccctgcacca tttaaaaaag aagaagtagt ttagagtttt atattttttg
    36661 taatgttaat ataaatttgc tataatagga ccatggaaga agaacaatta ttaaaatcag
    36721 gagagcgcat taaccagctc ttttcgacag atatcaaaat cattcaaaat agagaggttt
    36781 ttagctattc ggtggatagt gttctcttat cacgatttcc acgttttcct aagaaggggt
    36841 tgattgtgga tttctgtgct gggaatggag cagtggggct ttttgctagc actcgtactc
    36901 aagcacagat attgtctgtt gagattcagg agcgtttggc ggatatggct gaacgctctg
    36961 tccgtttgaa tggattagag gagcaaatgc aggtcatctg cgatgatttg aaaaatatgc
    37021 ctgctcacat tcaggggagt aaggtggata tgattttgtg taatccaccc tatttcaagg
    37081 tggatcctta ttctaatctg aacgagagtg aacattatct cttggctcga cacgaaatca
    37141 cgactaattt ggaagaaatc tgtcatagtg cccagagtat tctcaagtct aatgggcgtt
    37201 tggccatggt tcatcgtcct gatcgacttc tggatatttt ggatacgtta aaacgacata
    37261 atctagcccc taagcgcctg cagtttgttt atccaaaaag agaaaaggaa gccaatatgc
    37321 ttttgattga ggcgatcaag gatggctcaa caagtggctt taaggtctta cctcctttga
    37381 ttgtccacaa tgatgatggc tcttatacgc ccgaaatcga agagatttat tatggatcat
    37441 aaggcttata tgtacgttct ggagtgtcgt gatggatcct actatatagg ctatacgact
    37501 gatatgagaa gacgcctcgc tatccacaat agtgggaagg gagccaaata tacacgagca
    37561 cgcttgccag tcaaacttat ctatgctcaa ggttttgcca gtaaggaaga agccatgtcg
    37621 gctgaagctc ttttcaagcg taagaagagg ccacagaagg aagaattttt atctgaaaat
    37681 caagatagaa atttactcag ttattttgaa gaatcgtggg gagtcctttg actcctctta
    37741 cctttgtcaa aaagcgaaaa aaatgctaca ataaagaaaa taaacaaaat gaggtattat
    37801 catgtccaag attctagtat ttggtcacca aaatccagac tcagatgcca tcgggtcatc
    37861 tgtagctttt gcctaccttg caaaagaagc ttacggtttg gatacggaag ctgttgccct
    37921 tggaactcca aatgaagaaa cagcctttgt cttgaactat tttggtgtgg aagcaccacg
    37981 tgttatcact tctgccaaag cagagggggc agagcaagtt atcttgactg accacaatga
    38041 attccaacaa tctgtatcag atatcgctga agtagaagtt tacggtgttg tagaccacca
    38101 ccgtgtggct aactttgaaa ctgcaagccc actttacatg cgtttggagc cagttggatc
    38161 agcgtcttca atcgtttacc gtatgttcaa agaacatggt gtagctgtgc ctaaagagat
    38221 tgcaggtttg atgctttcag gtttgatttc agataccctt cttttgaaat caccaacaac
    38281 acacccaaca gataaaatca ttgctcctga attggctgaa ttggctggtg taaacttgga
    38341 agaatatggt ttggcaatgt tgaaagctgg taccaacttg gctagcaaat ctgctgaaga
    38401 attgattgac atcgatgcta agacttttga actcaacgga aataatgtcc gtgttgccca
    38461 agtgaacaca gttgacatcg ctgaagtttt ggaacgccaa gcagaaattg aagctgcaat
    38521 gcaagctgcc aacgaatcaa acggctactc tgactttgtc ttgatgatta cagatatcgt
    38581 caactcaaac tcagaaatat tggctcttgg tgccaatatg gacaaggtcg aagcggcttt
    38641 caatttcaaa cttgaaaaca atcatgcctt ccttgctggt gccgtttcac gtaagaaaca
    38701 agtggtacct caattaactg aaagctttaa tgcgtaagat tttgggtgtc agctcaaaat
    38761 cggaaagtct agtttgcctt atatcgaaag gagtttcggc tccttttttt ctaggagtga
    38821 agtatgttag aaaatggcga tttgattttt gtgagagatg ggtcagacat gggacaggcc
    38881 atccagactt ccacaggtaa ctatagccat gttgccattt atttggatgg gatgatttat
    38941 catgctagtg gacaggctgg tgttgtctgt caagaaccgg cagacttctt tgagtccaat
    39001 catttgtacg acctctatgt ttacccagaa atggatatcc agtcggtgaa ggaaagagct
    39061 tgcagacatc ttggagcacc ctacaatgct tctttctatc cagatgcagc tggtttctac
    39121 tgctcccagt atatagcaga aatcctacct atttttgaaa ctattcctat gaaatttgga
    39181 gatggggaac aggagattag tgatttttgg agggagtatt acatagaact aggtctgcct
    39241 gttcctctga accaagctgg gaccaatcct agtcagttgg cagcatcgcc tctgttacaa
    39301 tgtaaagaaa ggaatcttca tgattcagat ttttaatcca tctcgtttga cgagacagcc
    39361 atttttggag aattgatccg ctatctggac cagtatgagg atgtgattct acgggaaatt
    39421 aaggctcaat ttccagatgt tgcagttgat aaactcatgg aagagtatat aaaggcaggc
    39481 ttgattctac gtgaaaataa gcgctattac ctcaattttc ctacgcttga atcacttgat
    39541 agtcttgaac tggatcaaga gatttttgtc agagaagcta gtccggtcta tcaagccttg
    39601 ttggagcaga gttttgagac ggaattgcgc aatcaaatca atgcagctat tttagttgaa
    39661 agacggactt tgcgcgcatt aaaatgaccc tgtccaatta tttttacaag gtcaaacagc
    39721 agtatccttt gacagaaaaa cagcaggagc tctatgacat tttaggagat gttaatcctg
    39781 agtatgcact caagtatatg acggcttttt tgttgaaatt tctcaaaaaa gaccagctta
    39841 tgcagaaatg ccgtgatatc tttgtggaca gtttggttgt cttaggctat attgtgcaaa
    39901 atgaagatag aaagtatgag ttggctatcg attttgataa ggagagatta actttctact
    39961 tagcgtgatt tcttgtttct gagcacattg tttgactttc cttagtattc ggtataaact
    40021 atatgtaacc ggtaacacat atcggaataa actaaaggag acaatcatat gtcacttgaa
    40081 aacaaattgg aacaagcaac aggcgctgtc aaagaaggtt ttggtaaagt tactggagac
    40141 agcaagacag aacttgaagg agctgttgaa aaaacagttg ctaaggcaaa agacgttgta
    40201 gaagacgcaa aaggtgctgt agaaggtgcc gttgaaggtt tgaaaaacgt ttttactaaa
    40261 taataggaaa aaatcaaggg tttcattttc ccttgatttt ttctattctt ataaataatt
    40321 ttctgcgacg gctgtatctc ctgggtagga ttctttcttg ccctggatga tttggtaaca
    40381 atcggctccc ttacccgcaa taataactgc atctaattcg tgatttgtga tagccattgc
    40441 cgccttgatg gcttcttggc gatccgcaat cttttcaaca ggatgattga tgtagctact
    40501 aatttcatct gcaatggcca ttgggtcttc atagttaggg tcatcagcag tcagaaagac
    40561 ttgaatctca gggtgttgat tgaggaggag gccaaagtcc ttacgacgac tttctccctt
    40621 gtttcctgtt gatcccagaa ccagagcaat ctttccggtt tgatgagttt caaccacatt
    40681 gatgagtttt ttcagactat ccccattgtg ggcatagtcg atgaagacct tggctccatt
    40741 tttctgagtg aggacttcca tacgaccagg aacgcgggtt gcagcgatgc cttttttgat
    40801 gtcctcaaga cttgctccga gacggagaca agcaagtcca gcagcaactg cattttcttg
    40861 gttgaagttg ccaatgagtt ggatatcata atctccagcg agtttacccg tagctgaaaa
    40921 gctaaaggct ttggaattct cgatttggtt atcaaattgg ctaccataga aatcatggtc
    40981 ttgatcttca acctgttctt tcaagactga gaagtggtcc atgtcactgt taatgatgac
    41041 tgctcggcta ttttccatca agagacgctt gtggtagaaa tagtcttcaa agctagggtg
    41101 ttcaatcggg ccgatatggt caggagtgat gttaagaaag actcctacat caaaggtcag
    41161 tccatagact cgatggacta gataggcttg actggagact tccatgatga ggtgggtacg
    41221 gtcattttgc acagcctgat tcatcatgtc aaagaggtca atactctcag gggttgtcaa
    41281 cgctgactta aagaaagtct cgccatcaag agttgtgttc atggtcgaca acatagcagg
    41341 tctatgccct tgagataaga tgttataggc gaaataggtt gctgttgtct tacccttagt
    41401 accagtaaag gcaaggagtt tgagtttctc ttgtggatta ccatagaact ccatggcaat
    41461 caaactcatg gctttcttta tatcgttcac aatgatgaca gggatatcga cttcgtagtc
    41521 cttttcagct acataccaag ctaaaccttg tgttatagca gaaagaaggt attctttttt
    41581 aaaggcagcg ccttttgcaa aaaaaagagt gtcttctgtt acttttcggc tgtcgtagct
    41641 gatgctatca aaaataactt tgctgtagtt gtagtggtaa tgaccttggt caataatttc
    41701 gcgaaaaagg ccatctttct ttaaaatatc taatacggtt tcaatcttaa tcatactttc
    41761 tattgtaaac cgaaagtcgt aaatttacaa gtaacaagga aaagtttata atggaagata
    41821 aggagttttt cctagttatc aaaattgaat gaggaatcta tgtcgcacga aaacaatcac
    41881 cagcaggccc agatgttacg ggggactgct tggctaacgg ctagtaactt tatcagtcgc
    41941 ctactcgggg ctgtttacat tatcccttgg tacatctgga tgggggctta tgcagctaag
    42001 gcaaatggtc tctttaccat gggttacaat atctatgctt ggttcttgtt ggtttcaaca
    42061 gcggggattc cagttgcggt ggccaagcaa gttgccaagt ataataccat gcgagaagaa
    42121 gagcatagct ttgccctgat tcggagcttc ttaggcttta tgacaggact aggcctggtt
    42181 tttgctttag tcttgtatgt ctttgctcct tggctagcag acttgtctgg cgtgggcaaa
    42241 gacttgatcc caatcatgca aagcttggct tggggagtct tgattttccc gtctatgagt
    42301 gttatccgag gatttttcca agggatgaat aacctcaaac cctatgccat gagccaaatt
    42361 gctgagcagg tcattcgtgt tatctggatg ctcctagcaa cctttatcat tatgaagctc
    42421 ggttcaggag attatctagc agccgttacc caatcaacct ttgctgcctt tgtcggtatg
    42481 gtagccagtt ttgcagtctt gatttatttc cttgcccaag aaggttcact caaaagagtc
    42541 tttgaaacag gagataagat taacagtaag cgtctcttgg ttgataccat taaggaagcc
    42601 attcctttta tcctgacagg gtctgccatc cagctcttcc agattttgga tcagctgacc
    42661 tttatcaata gtatgagctg gtttaccaac tacagcaatg aggacttggt tgtcatgttt
    42721 tcttatttct cagccaatcc taataaaatc acgatgattt tgatttctgt aggggtttcg
    42781 attgggagtg ttggtttgcc acttttgacg gaaaactatg tcaaggggga cttgaaagcg
    42841 gcttctcgtc tcgttcagga cagtctcacc ctactcttta tgttcttgct accagcaacg
    42901 gttggagtgg ttatggtagg agaacctctt tatacggtct tctatggtaa gccagatagt
    42961 ttggctctgg gcttatttgt ctttgcagtt ttgcagtcta ttattttagg cttgtacatg
    43021 gtcttgtctc caatgcttca ggccatgttc cgcaaccgca aggccgttct ctattttatc
    43081 tatggttcta ttgccaagct agtcttgcaa ctacctacca tcgccctctt ccacagttat
    43141 ggtcctttga tttcaacaac cattgctctc atcattccta acgtcttgat gtatcgggat
    43201 atttgtaaag taactggtgt caagcgcaag gtgattttga agcgaaccat tttaatcagt
    43261 ttgctgaccc tagtcatgtt tctgttaata ggaaccatcc agtggctgtt aggatttttc
    43321 ttccaaccaa gtggacgttt gtggagcttc ttttatgtag ctcttgtcgg tgccatgggg
    43381 ggtggacttt atatggttat gagtctgcgt acctatttat tagataaggt aataggaaaa
    43441 gcccaagcag atcgcctgcg agcaaaattt aagctttcgt aaggtagcat ttataaataa
    43501 aaggaataag ttgaacaatc ttttaggaga acttgaaact tattcctttt ttagcgtcaa
    43561 aatagctaaa attatctttc aaaaatagaa aatgaggttt ttttgatttt ttggaaaaaa
    43621 caattgacta aacaaaccaa gagttgtata ataaaacaaa tatttaaagc aggaggttct
    43681 ggaaatgaaa aagtctaaga gcaaatatct aaccttggca ggtcttgtcc tgggtacagg
    43741 agttttattg agcgcgtgtg gaaattctag cacggcgtca aaaacataca actatgttta
    43801 ttcaagtgat ccatctagtt tgaactatct agcagaaaac cgcgcagcaa catccgatat
    43861 tgttgcaaat ttggtagacg ggttattaga aaatgaccaa tatgggaata ttattccatc
    43921 attagcagag gattggactg tttctcagga cggtttgacc tatacctaca aacttcgtaa
    43981 ggatgccaag tggtttactt ctgagggaga agaatatgcg cctgtaactg cccaggattt
    44041 tgtgacaggt ttgcaatatg cagctgataa aaaatcagaa gctttgtatc tagtgcagga
    44101 ctctgttgct ggtttggatg actatatcac tggtaaaaca agcgactttt caactgtcgg
    44161 tgtcaaggca cttgatgacc aaacggttca atatactttg gttaaaccag aactttactg
    44221 gaattcaaaa acacttgcaa cgatactttt tcctgttaat gcagatttcc tgaaatcaaa
    44281 aggggatgat tttgggaagg cggatccatc tagtattttg tacaatggac ctttcttgat
    44341 gaaagcactt gtctcaaaat ctgctattga atataagaaa aaccctaatt actgggatgc
    44401 taagaatgtc tttgtagacg atgtgaaatt gacctactat gatggtagcg accaagaatc
    44461 actggaacgt aattttacag ctggtgctta tactacggct cgtctttttc ctaacagctc
    44521 cagctatgaa gggattaaag aaaaatacaa aaacaatatc atctatagta tgcaaaattc
    44581 aacttcatat ttctttaatt ttaacctaga taggaagtct tacaattata cttctaaaac
    44641 aagtgacatt gaaaagaaat cgacccagga agcagttctc aataaaaact tccgtcaggc
    44701 tatcaatttt gcttttgaca gaacatctta tggggctcag tctgaaggga aagaaggtgc
    44761 aacaaagatt ttgcgtaacc tagtggttcc tccaaacttt gtcagtatca agggaaaaga
    44821 ctttggtgaa gttgtagcct ctaagatggt caactatggt aaggaatggc aaggtatcaa
    44881 ctttgcggat ggtcaagacc cttactacaa tcctgagaaa gccaaggcta agtttgcgga
    44941 agctaagaaa gaactcgaag caaagggtgt tcaattccca atccacttgg ataagactgt
    45001 ggaagtaaca gataaagtag gcatacaagg agttagttct atcaaacaat caattgaatc
    45061 tgttttaggt tctgataatg tagtgattga cattcagcaa ttaacatcag atgagtttga
    45121 cagttcaggc tactttgctc aaacagctgc tcagaaagac tatgatttat atcatggcgg
    45181 ttggggacct gattatcaag acccgtcaac ctatctcgat atttttaata ctaatagtgg
    45241 aggagttcta caaaatcttg gactagagcc tggagaagcc aatgacaagg ctaaggcagt
    45301 tggactggat gtctatactc aaatgttgga agaagctaat aaagagcaag atccagccaa
    45361 acgttatgag aaatatgctg atattcaagc ttggttgatt gatagttctt tagttcttcc
    45421 aagtgtttcg cgtgggggaa caccatcatt gagaagaacc gtaccatttg ctgctgccta
    45481 tggtttaacc ggtacaaaag gggttgaatc atataaatac ctcaaagtac aagataagat
    45541 tgtcacaaca gacgaatatg caaaagccag agaaaaatgg ttgaaagaaa aagaagaatc
    45601 caataaaaaa gcccaagaag aattggcaaa acatgtcaaa taaaatctaa ttgtagataa
    45661 gtttgtgtaa gaagtctttg tgcttaaaaa cgcataatat caggtatata aaatcctaga
    45721 tgttatgcgt tttgttatag gaagatttat tagattttat cctcaaattg agttattccc
    45781 gacttt
//
LOCUS       DAVGJL010000004        41747 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.004, whole genome shotgun sequence.
ACCESSION   DAVGJL010000004 DAVGJL010000000
VERSION     DAVGJL010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 41747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41747
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.004"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(<1..247)
                     /locus_tag="VVE42_000164"
     CDS             complement(<1..247)
                     /locus_tag="VVE42_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3072436.1"
                     /translation="MNQLEFQRNHLQMDYYSESYQDFERDFYRYSNMNIPLTFLTDDI
                     LKTMATSRKNYFVLNKEKSRDNRDHFFIFEVSTVDENP"
     gene            complement(234..602)
                     /locus_tag="VVE42_000165"
     CDS             complement(234..602)
                     /locus_tag="VVE42_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3072437.1"
                     /translation="MIDRSYLPFQSAREYQDTKMQKWMGFFLSEHASALSDDTNKVTY
                     MSDLSLEKKLLLLSQVYAGQLRTRIQVIEKNKRVSYTGTIPSLTKDFILIKTTTGHIN
                     LKLKDIISIELVEEVLYESA"
     gene            complement(615..950)
                     /locus_tag="VVE42_000166"
     CDS             complement(615..950)
                     /locus_tag="VVE42_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imsC-like protein"
                     /protein_id="HEU3072438.1"
                     /translation="MKQEKNTVQFSEIRSKGCNDIEMLERFLHGIVETATSKLRQRKL
                     KTTEISIRLVHAKSENRLPLEFTFSIKPTSSSVIIYTEVINRFKECYTGGGIQGFTIQ
                     FDKNTLASA"
     gene            complement(947..1192)
                     /locus_tag="VVE42_000167"
     CDS             complement(947..1192)
                     /locus_tag="VVE42_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3072439.1"
                     /translation="MRQIGVSYSGFVDESYTLLSLFDDVEQIEKDNRLQTAIDVVREQ
                     FGFLAIQKGTVLTEGSRNIERSKLIGGHSAGGLEGLK"
     gene            complement(1556..3475)
                     /gene="tet(M)"
                     /locus_tag="VVE42_000168"
     CDS             complement(1556..3475)
                     /gene="tet(M)"
                     /locus_tag="VVE42_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEU3072440.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSATHEIILSFLG
                     KVQMEVTCALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYTDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            complement(3794..4726)
                     /locus_tag="VVE42_000169"
     CDS             complement(3794..4726)
                     /locus_tag="VVE42_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEU3072441.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            complement(4723..5724)
                     /locus_tag="VVE42_000170"
     CDS             complement(4723..5724)
                     /locus_tag="VVE42_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEU3072442.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            complement(5721..7904)
                     /locus_tag="VVE42_000171"
     CDS             complement(5721..7904)
                     /locus_tag="VVE42_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEU3072443.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTALGAGSAIASATGKQGQSGSGSSARTQADHSRPD
                     GKEKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENV
                     RDLTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQE
                     QYHDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATST
                     TTADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            complement(7907..10354)
                     /locus_tag="VVE42_000172"
     CDS             complement(7907..10354)
                     /locus_tag="VVE42_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU3072444.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISM
                     IGENQVNYRFFIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLDN
                     ETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILTFLTGISSRDSDR
                     FPVLRKAIRAVTNSEVRGLLKVIEELRVEGTQISTSIADHIESFTDYDFAHLLFSDGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            complement(10338..10844)
                     /locus_tag="VVE42_000173"
     CDS             complement(10338..10844)
                     /locus_tag="VVE42_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEU3072445.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     YTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            complement(10819..11316)
                     /locus_tag="VVE42_000174"
     CDS             complement(10819..11316)
                     /locus_tag="VVE42_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEU3072446.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            complement(11433..11654)
                     /locus_tag="VVE42_000175"
     CDS             complement(11433..11654)
                     /locus_tag="VVE42_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3072447.1"
                     /translation="MNFGQNLYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLI
                     IAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            complement(11697..12902)
                     /locus_tag="VVE42_000176"
     CDS             complement(11697..12902)
                     /locus_tag="VVE42_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEU3072448.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            complement(12925..13077)
                     /locus_tag="VVE42_000177"
     CDS             complement(12925..13077)
                     /locus_tag="VVE42_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEU3072449.1"
                     /translation="MLAGVAIATPALNPPYLTGGYKSTGNSQKNIRKSFGYKGFTKFQ
                     RMSNGL"
     gene            complement(13080..14465)
                     /locus_tag="VVE42_000178"
     CDS             complement(13080..14465)
                     /locus_tag="VVE42_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEU3072450.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            complement(14494..14880)
                     /locus_tag="VVE42_000179"
     CDS             complement(14494..14880)
                     /locus_tag="VVE42_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEU3072451.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            complement(14896..15210)
                     /locus_tag="VVE42_000180"
     CDS             complement(14896..15210)
                     /locus_tag="VVE42_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEU3072452.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            complement(15614..16264)
                     /locus_tag="VVE42_000181"
     CDS             complement(15614..16264)
                     /locus_tag="VVE42_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEU3072453.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLE
                     HTVSELFVERLEKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRVHD
                     SFDILHYLDKHSLHIGDQLQVQQFDGFSNTFTILSKEEDLQVGMDIAKQLYVEKID"
     gene            16376..17218
                     /locus_tag="VVE42_000182"
     CDS             16376..17218
                     /locus_tag="VVE42_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEU3072454.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADARQLGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHTGKQEDFDRLVTHPPCDIAVY
                     GHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDDKGLIDMDFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            17294..17731
                     /locus_tag="VVE42_000183"
     CDS             17294..17731
                     /locus_tag="VVE42_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEU3072455.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKESKVTSDFLAASVGVNPVIIRK
                     ILSQLKKAELISVARGTGGTEIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQTSLEKVVADAESQMKD"
     gene            17800..18102
                     /locus_tag="VVE42_000184"
     CDS             17800..18102
                     /locus_tag="VVE42_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3072456.1"
                     /translation="MYLVIGIVLAFIVSFWKDNRSLWNPVLFLLSLISSCFYLSYLFY
                     KNGYENVQLAFYVFAFVLLPFLLFLSGIFLIYNGVILLKREGRSKPHYLSMLFDFY"
     gene            complement(18193..19266)
                     /locus_tag="VVE42_000185"
     CDS             complement(18193..19266)
                     /locus_tag="VVE42_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEU3072457.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLDLAPQVPREPTMLTSKNRLQLLDKLAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEDFHLTYMKEIPAQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHQIIVQSTALGGIAQHDTFSWQIEDKHFVQLDKTNSDSRQVDT
                     TFKEWVTTVPDEELQLYFDLFFGTILDAGISSINDLASLKALEYIHHLFVQAQSLTPE
                     EKETLGRLTQLLIDTRYQVWKNR"
     gene            19422..20099
                     /locus_tag="VVE42_000186"
     CDS             19422..20099
                     /locus_tag="VVE42_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU3072458.1"
                     /translation="MHKILLIEDDQVIRQQVGKMLSEWGFEVVLVEDFMEVLSLFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDDLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            20092..21066
                     /locus_tag="VVE42_000187"
     CDS             20092..21066
                     /locus_tag="VVE42_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEU3072459.1"
                     /translation="MLDWKQFFLAYLRSRSRLFIYLLSLAFLVLLFQFLFASLGIYFL
                     YFFFLCCFVTILFFTWDILVETQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLVLKQVQIEDLVKEIIRKYALFFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(21115..23058)
                     /gene="thrS"
                     /locus_tag="VVE42_000188"
     CDS             complement(21115..23058)
                     /gene="thrS"
                     /locus_tag="VVE42_000188"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEU3072460.1"
                     /translation="MINITFPDGAVREFESGVTTFEIAQSISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHEDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEARKIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFDKKDLKNYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRE
                     LERYIVNKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVDYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVDYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEGETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(23218..23439)
                     /locus_tag="VVE42_000189"
     CDS             complement(23218..23439)
                     /locus_tag="VVE42_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEU3072461.1"
                     /translation="MELQELVERSWAIRQAYNELEVKHHDSKWTVEEDLLALSNDIGN
                     FQRLVMTKQGRYYDETPYTLEQKLSENIW"
     gene            24296..24925
                     /locus_tag="VVE42_000190"
     CDS             24296..24925
                     /locus_tag="VVE42_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU3072462.1"
                     /translation="MKLAVIAANGQAGKAIVEEAVKRGHEVTAIVRSENKSQAESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKSIELFNQLLAGTQTRFLVVGGAGS
                     LYIDETKTTRLLDTPDFPEEFKPLAKAQADEFDLLRTKNNLNWTFVSPAVDFIPDGEK
                     TGNYILAGEIFTTNEKGISQISYADYAIGLVDELEKGHHIKERISLLEK"
     gene            25066..25335
                     /gene="rpsO"
                     /locus_tag="VVE42_000191"
     CDS             25066..25335
                     /gene="rpsO"
                     /locus_tag="VVE42_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEU3072463.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
     gene            26069..26683
                     /locus_tag="VVE42_000192"
     CDS             26069..26683
                     /locus_tag="VVE42_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEU3072464.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAIVGEDAEEEEEEIIERL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(26976..27725)
                     /locus_tag="VVE42_000193"
     CDS             complement(26976..27725)
                     /locus_tag="VVE42_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEU3072465.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTDKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINV
                     LKKSDRSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMTLKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            27858..30194
                     /locus_tag="VVE42_000194"
     CDS             27858..30194
                     /locus_tag="VVE42_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEU3072466.1"
                     /translation="MDKNKIMGLTQREVKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNLAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKPINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLTETYGEEAIASILTSYMA
                     HSEDKNPTAQAIRQRFVGDVAYPMISNLPFSSDRKWGAMELEGLGTVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDIQALALLEILDPIREGAAETLDY
                     LRSQEVGLKIISGDNPVTVSSIAQKAGFADYHSYVDCSKITDEELMAMAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEPNFLRRSMLRALPSALMVVFSVLFVKIF
                     GTSQGWSELEISTLLYYLLGSIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFPRI
                     QKLLEISTLTEQTLPVYGVMMLVFTVIFILTSRYQARK"
     gene            30296..30973
                     /locus_tag="VVE42_000195"
     CDS             30296..30973
                     /locus_tag="VVE42_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEU3072467.1"
                     /translation="MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQIKSAIA
                     GEVVEGPYQSAVKNVEAHGLKEKIQVRLANGLAAFEETDQVSVITIAGMGGRLIARIL
                     EEGLGKLANVERLILQPNNREDDLRIWLQDHGFQIVAERILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLSKEVSPVFVQKWQKEAEKLEFALGQIPEKNLEERQVLVDKIQAIK
                     EVLHVSK"
     gene            30960..31757
                     /locus_tag="VVE42_000196"
     CDS             30960..31757
                     /locus_tag="VVE42_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEU3072468.1"
                     /translation="MLASEVIQAYEAFCPQEFSMEGDSCGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLGIEETTYLQETGPERGIGRIGNIQPQTFWELAQQVKQVFALDS
                     LRMVHYQENDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVLFVEKIAALLTQWKEKKGWELEILPSQASTNPFRHI"
     gene            31774..32877
                     /locus_tag="VVE42_000197"
     CDS             31774..32877
                     /locus_tag="VVE42_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU3072469.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVTVFDHEQGQATKAAAG
                     IISPWFSKRRNKAWYKMARLGADFYVDLLADLEKSGQKIDFYQRSGVFLLKKDESKLE
                     ELYQLALQRREESPLIGQLAILDQASANELFPGLQGFDRLLYASGGARVDGQLLVTRL
                     LEASHVKLVKEKVTLTLLSSGYQIGEEVFDQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLAQDMEDYPVVMPEGEWDLIPFADGKLSLGATHENDMGFDLTVDEPLLQQME
                     EAALPHYPVLARATSRAERVGIRAYTSDFSPFFGQVPELAGVYVASGLGSSGLTTGPI
                     IGYHLAQLIQDKELTLDPENYPIENYVKRVKSE"
     gene            32884..33120
                     /locus_tag="VVE42_000198"
     CDS             32884..33120
                     /locus_tag="VVE42_000198"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3072470.1"
                     /translation="MKIKEQTRKLAAGYSKHCFEVVYRTDEVSSKHCFEVVYRTDEVS
                     SKHCFEVVYRTDEVSNHTHGKATLTWFELDFRRV"
     gene            33223..34242
                     /gene="galE"
                     /locus_tag="VVE42_000199"
     CDS             33223..34242
                     /gene="galE"
                     /locus_tag="VVE42_000199"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU3072471.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVERITGVEIPFYEADIRDTVTLRDIFKQEEPTGVIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKAMEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            34244..35197
                     /locus_tag="VVE42_000200"
     CDS             34244..35197
                     /locus_tag="VVE42_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU3072472.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAVHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFCAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTHYLDYPNVERQAGKTSWSFRQLFFYSIE
                     GIVNFSDFPLTIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSDLPDFTEKIK"
     gene            complement(35259..35465)
                     /locus_tag="VVE42_000201"
     CDS             complement(35259..35465)
                     /locus_tag="VVE42_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEU3072473.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDDPDQLEKEI
                     SPSQDVLEAVKNCPTRALIGNQEA"
     gene            35517..35993
                     /locus_tag="VVE42_000202"
     CDS             35517..35993
                     /locus_tag="VVE42_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEU3072474.1"
                     /translation="MAKEPWQEDIYDQEESRAGRRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKDFHDSDASVVQISSSSSSQAEQSSEPESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMATGSWFANPGDVIKIK"
     gene            36002..36673
                     /locus_tag="VVE42_000203"
     CDS             36002..36673
                     /locus_tag="VVE42_000203"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEU3072475.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLGVEEVEALLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPEVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDLETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            36804..37142
                     /locus_tag="VVE42_000204"
     CDS             36804..37142
                     /locus_tag="VVE42_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEU3072476.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLTDGDTVTLIKDLKVKGAPKDLKQGTRVKNIRIVEGDHNIDCKIDGFGAM
                     KLKSEFVRKI"
     gene            37145..37774
                     /locus_tag="VVE42_000205"
     CDS             37145..37774
                     /locus_tag="VVE42_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HEU3072477.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITRHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
     gene            38014..39165
                     /locus_tag="VVE42_000206"
     CDS             38014..39165
                     /locus_tag="VVE42_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU3072478.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGTRLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVLPLYLLFVPYGKSKKEVKKRAKEASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
     gene            39478..40227
                     /gene="truA"
                     /locus_tag="VVE42_000207"
     CDS             39478..40227
                     /gene="truA"
                     /locus_tag="VVE42_000207"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEU3072479.1"
                     /translation="MTRYKATISYDGYAFAGFQRQSHARSVQEEIEKTLTRLNKGQTI
                     TVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVADDFHC
                     RYAKHSKTYEFIVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IDLILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            40217..40990
                     /locus_tag="VVE42_000208"
     CDS             40217..40990
                     /locus_tag="VVE42_000208"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEU3072480.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASH
                     LIKGDKLLPAVESSKAFVYRAIAQADQYGVRQYEANKNN"
     gene            40968..41429
                     /locus_tag="VVE42_000209"
     CDS             40968..41429
                     /locus_tag="VVE42_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU3072481.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKIPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLIDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     ATIAMVGGYALGSTLMNGWAAALPEILPNFMQNMVGMIVGFILSQSIKKIK"
BASE COUNT        11787 a         8159 c         8847 g        12954 t
ORIGIN      
        1 gcggattctc atctacggta cttacttcaa atatgaagaa gtgatcgcgg ttatctctgg
       61 acttttcctt attgaggaca aagtaattct tacgtgaagt cgccattgtt tttaggatat
      121 catcagttag gaaggtcaat ggaatattca tgttagagta gcggtagaag tcacgttcaa
      181 aatcttggta gctctcgcta taatagtcca tttgtaggtg attacgctga aactcaagct
      241 gattcataga gcacctcctc gacaagttca atactaataa tgtcttttaa tttcaaattg
      301 atgtgacctg ttgtagtttt tatcaaaatg aaatctttgg tcagacttgg tattgttcca
      361 gtgtaggaaa cacgcttgtt tttttcaatc acttgaatgc gtgtgcgtag ctgcccggcg
      421 tatacttgac tgaggagtaa taatttcttc tctagtgata agtcagacat gtacgttact
      481 ttgtttgtat catcagagag tgctgatgca tgttcagata ggaaaaagcc catccatttt
      541 tgcatctttg tatcctggta ctctcttgct gattgaaatg gtaaatatga acggtcaatc
      601 atatcaaatc ctttctatgc agaggcaagg gtatttttat caaattgaat cgtaaaacct
      661 tgaattcccc cacctgtgta acattcttta aagcgattga ttacctcagt atagattatc
      721 acagatgagc ttgttggctt aatgctaaat gtaaattcca atggtaatcg gttttcagat
      781 ttagcatgta ctagtcgtat cgatatttca gttgttttga gttttctctg acgaagtttt
      841 gaagttgctg tttcaacgat tccatgtaaa aatctttcaa gcatttcaat atcattacat
      901 cctttgctac ggatttctga aaattgtact gtattttttt cttgtttcat tttaatccct
      961 ccaatccacc cgcggaatga ccaccgataa gtttactgcg ttcaatattt ctggaacctt
     1021 cagttaggac ggttcctttt tgtatggcta aaaaaccaaa ctgttctctg acaacatcaa
     1081 tagctgtctg aagtctatta tctttttcaa tttgttctac atcatcaaag agtgatagta
     1141 gagtatagct ttcatctacg aagccactat aagatacacc aatttgtctc actgcaccag
     1201 aggtgtattt tttcggaata atacaagtac atgactcacc attgttttgg ggagatttgc
     1261 gggttcaatt ttattctgag catttataga ttttttcatc tcagtcctag aatagccaat
     1321 atgaatagaa acgacagtag tcaatactag ggctactgtt ccgttccaca gtatcattta
     1381 aaaatcattt tcacaccctt tcgtctatta gtatagaaga aagctctcag cacacaatat
     1441 ccacttgtag tttataataa ctatctcctc ctttacactt taattcaaat ctttattaaa
     1501 aaatatttca tcttatttaa caagaaacca tatttatata acaacataaa atacactaag
     1561 ttattttatt gaacatatat cgtactttat ctatccgact atttggacga cggggctggc
     1621 aaacaggttc accggtagta acatggtacc cttttaactc tgttaaacaa acactacgtc
     1681 catttgtaaa gaaagttaaa tcactacgat attcttgaat acaccgagca gggatttctc
     1741 cactaagaat gacctcatta tttttcaatt gagtgtctac gatgttcgca caatatttag
     1801 gagcatcggt gtatgctcgt gaaagatatt cctgtggcgc ataaatttta aaactaagat
     1861 atggctctaa caattctgtt ccagcttttt ttaagacttg ttccaataca ataggagcaa
     1921 gcatccgaaa atctgctggg gtactaacag ggctatagta taagccatac ttaaaacaga
     1981 ttttacagtc cgtcacattc caaccataca atccttgttc acagccatag cgtatccctt
     2041 ccataactgc attttgaaat gattgattta agtatccaag agaaaccgag ctctcatact
     2101 gcattccact tcccaacgga agcggtgata cagataaacc aatggaagcc cagaaaggat
     2161 tcggtggaac ttcgatgtga atggtatact ctgctttttt taacggtctt tccatataaa
     2221 tgactgtagg ctcttttatt tctatctcca catgatactt ttcttgcagc agagcacaag
     2281 tcacttccat ttgtactttc cctaagaaag aaagtatgat ttcatgtgtc gcagaatcca
     2341 cataatatcg cagaagcggg tcactgtcgg agatttctaa aagtgcatca agcaacattt
     2401 ctctctgttc aggtttactc ggttcaacag ttgtttgtag tagagggtgc ggattttcaa
     2461 tcttttttct ctgtggcaat agttttgtat ctccaagaac actatttaac ttcaaaaact
     2521 cattttgcaa aataacaatt tctccagaat aagctctatc aatcttacat aattcaccat
     2581 ttattgaagt atacatttct gtaactttta ttttttcttt ttctgatact ctaaccgaat
     2641 ctcgtaaatg tagtactcca ctataaaggc gtatatatgc aagacgttgt cttttttttg
     2701 tatattcaat tttgaaaaca tttccgcaaa gttcagacgg acctcgatgt gttgatgaat
     2761 aaaatttatt cgtaatcact tctataaggt tatcaatccc tatattgttt tttgcacttc
     2821 cgtgataaag agggaacaga gaacaattct gaaatcttat gctttcctct tgttcgagtt
     2881 ccaatgcttc taatgattta ccggacatat atttctctaa aaggtcatcg tttccctcta
     2941 ttaccgtatc ccattgttca gattcggtaa agttcgtcac acacatatta ggatacagtt
     3001 ctaccttctg tttgattaca atttcggcag aaagtttctc tttaatatcc tgataaaccg
     3061 ttgataaatc aattccattt tggtcaatct tattgataaa aaagattgtg ggaatcccca
     3121 ttttcctaag tgcatgaaat aatatacgag tttgtgcttg tacgccatct tttgcagaaa
     3181 tcagtagaat tgccccatct aaaactgata atgaacgata tacttctgct aagaaatcca
     3241 tatgtcctgg cgtgtctatg atgttcacct tcgtattttc ccactgaaaa gaggttattc
     3301 ctgtctgaat tgtaattcct ctctgacgtt ctaaaagcgt attatccgtc ctcgttgtac
     3361 ctttgtccac gcttcctaat tctgtaatcg ctccactgtt atataataag ctttctgtta
     3421 aggtagtttt tcctgcatca acatgagcta aaactccaat attaataatt ttcatgtgat
     3481 tttcctccat tcaaaaaccc aaaagggcat aaaaagcata cagatattct ccggatactt
     3541 tagaatcaca tgataaaggt attcttaaac tgggtacaaa aaactaagcc ctcctaaaaa
     3601 aggacatcca attatttgtt cccactatca aattgacagt ttatttaaga ataccttgcc
     3661 gcatatttat taactccttt taaatagata cttaaataat agcacataag agcatatttg
     3721 tcaaggaatc tccaattttt tatcaaagag agtacgtgat tacaaaatag ctgtaataat
     3781 gtaccaatat ttgttattct ataatcttcc aattactccc gttcttttca agtaccaaat
     3841 caaattgaga tacctgcgtt gctttggtct gctggtcgat atactccact gtcagcgata
     3901 ccgtgacttg attatcctta cgattgtgaa taggatttac cagttcttga aagatgtact
     3961 cttttccgat tggttttaat atcccgtcat tcacatagta ggaaagttca ctggctgtcg
     4021 ctgtaggata gagcttgaag aacgtcgtta aaaactcatt gatttcattg gttgtaatgg
     4081 aatcaaccgt cccctcactt tcaatggctt ttggtttata acttgatttc ttaggtatgt
     4141 tggtaatggt cggattctta accagtacca tatttccaga accatctaca tagacactca
     4201 ctatataagc agagtggacg gtctttgtat tttctccctc tgtaatgagc tggtctacac
     4261 tgtaggttac attaaactca ttgtcgccag ttggctctac cgtccatatc tgaaatcctc
     4321 ttacagaaga cgatacagga atatctttgc gtactgtatc aacattgaga gcttgaagtt
     4381 catctgtcag atagcctttt agactttcca ttcgattatc aatggactta tcggattgct
     4441 cccatgaata gtagactttc gcaaagttct ctacaaaatt ttctacatga tgagtatcaa
     4501 cgtattcctt ttctatgata gttgtttcgt gaatagtatg agtatctata gctgtaaagt
     4561 gcttgaatat cgcaaagctg aaactaagcc ctaaaagtac ccacaaggca atcacaacct
     4621 ttttatgagg attgacctta tagacacgag gtttcttttc ctttggtatc tgtttttctt
     4681 tattctgatt ttttctaaat ttcatcatta aatcttcctt tctcattgtt tgattcgtcc
     4741 tgctcccact aaatgctgtt gccagtaggg gcttgttaag tcggcataac cgattgggtc
     4801 gcctgcatga aacatacggt tattgccaag gtatatccca acatgagtaa tataagagcc
     4861 agcgttatag gtagaatgaa agaaaaccaa atcgccagct tgtgcttccg atagtgggat
     4921 atgctgggtc acatcatatt gctgttgtgc ggttcgtggt aagttaattc cagcttttcc
     4981 atacgtccat tgtgtcagtc cgctacaatc aaaagaagta gtcggggaag ctccaccgta
     5041 aacgtatcgc cagccctcat atttcagtgc ttcgtccatg atggcttgta ccgtatcatc
     5101 atcaaactct gttgtgacaa gatactgcgt taccagttgc acataaaaca tattgccata
     5161 gttgtatcgc cagcccccat tgataggtat ggctatggga ttggggtaag acactttttc
     5221 gccacctgaa tactcttttg agaaactttg agccagttca aaggtatatt tatttccacg
     5281 attagccaca taccctaaga aaccaccacc ataattgtag gactggataa ccgattctaa
     5341 atctacactg agcctttcgc tactggctaa taattcactg aaatacttca caccttgctt
     5401 aatggattct tctgtactca atgaattagg tggaagaccg agggattccg aggactgcat
     5461 aacatcttcc gcagtaccgc ccgattccac ctgtataatc gcaagaagta tgttgacata
     5521 ttcttcaacg ccatattctt tggcatattt ttctaccata ggcttatgag ccagcacttc
     5581 tgcggaaaca ttcacacctc cataatgaat attggaaatt ccgctgtcct gttcatctga
     5641 aaataaaatg gcaacaaaca gaagcagtga gaagaccatc aagaataatc cagaaccacc
     5701 aatcactaaa gttttcaact tcatggtttc ttaccgactt tcttaatggt ggcggttttg
     5761 attggtggtc tacttcttgt attttgtagt ggtactcttt gaacagtaga cggacgttct
     5821 tttgtgattg gacgttgtga agttctatct gctgtagtgg ttgaagttgc tggcttttga
     5881 acggtttttt cttgaacggt attgccttgg cgttccactt ttggacttga aaaatcggac
     5941 ttaactgctg gacgctcttg tttggcttgt tgagattcct tatatgaagt ctgaatatta
     6001 gactgttttg aggtctgttc atcatgatat tgttcttgtc ttgtagtcgg tctttcatga
     6061 acagaagaag caggctgttt tttctgtttg acctgttcca tttcagagcg acgcttcgca
     6121 atggtttttc gcctttgttc ctgctgttcc ttgcgtccac tggctctgtc cgctttggtt
     6181 tgagaaatac tactggttaa atcacggaca ttctctttta ctttggattt tccttgatat
     6241 actgcatatc ttgcattggt cggcaaatct ttaacctgtt ctttcaaacc actagcagtg
     6301 tctaccattc tgtctttggt atcagctact gtaccgatgg tttgaccgat acgttttcca
     6361 agtgttgatt tttcctttcc gtctggtcgg gagtgatctg cttgtgtcct tgcagaactc
     6421 cccgaacccg actgtccttg ttttcctgta gcactggcaa tggcagagcc agcacctaaa
     6481 gctgtcatgg agcgtccaag tttacgctgt aggcggtgca tatgagcgtg cataagcata
     6541 cgaggttttc tcatcacacg acttcccaca ctttgagaat cgttactctg tagagaaaac
     6601 atactcatta aatcgcccag cttgaagtag attcctgcaa aggtcacaat ctgtagaaaa
     6661 gcaatcaaaa agaacggata accagccgat aaggtataga gcatggttga aatactaaat
     6721 gctgtcgtaa taatcaatgt gattccagct cgtgtcaaaa tggtattaaa gagctttgtt
     6781 atggctcgtt ttgacatacc atcaaatgat ggaatcatgc ttaaaataaa gctcacaggc
     6841 agaaacatag catagatgat aaaaagtacc tgcgagaaaa tcatgattcc tgttaatagg
     6901 aatacaaata tggaaatccc aatattgaag acaaatagga agaagactgt acctaaacgg
     6961 ttaatggtct ttgtaatggt tagattggta ttgcttctgt cttcaatttc ttccgcaaca
     7021 attttttctc tgtcttcgcc attgttggaa tctgggctgg tggagagcag gctttccaca
     7081 cggtcaatac cgatactttc aatgtctgaa ctgttgtatt gaagcagtag ccacggttgc
     7141 tgaacctgta tggaaaacag gctatctctg attaagtcca cgctgtcctt gccttgacta
     7201 tcggaatggg gcatgacaat cttcgtgcca agtgataaac tggcattact gatgtctgat
     7261 gaaaagtcat tgattttttt aatgtagtcg ggagcgtagg caataaagga agccgatagg
     7321 ataaacacca gcacaaaatt cataatggca tgaattgcct ttgtggtttc tctctttatc
     7381 agtcccgtat aggcaacata aaccccaaga accaaaatca agagtaagag gaatccaaca
     7441 tagaaaccct ctgttgaaaa tccgtttgca ctcacaccag ctaaggtctg catattctta
     7501 ccaatggaat ctgctgtagc ggaaatgaag tctaaggaat aggcttcctg tactaagtaa
     7561 cctgtcgcat tggaaacata caaactgatt gtccaaataa aattggtaat ggcatatagt
     7621 ccatacatga cctgttttcc aatcccgtcc gaccagttcc acggaagcca gccccagcta
     7681 ttatccacat aaaaatccag ttgatagttt tcaagtgggt atcggctgta ttcatttgcc
     7741 acattgaccg tatcatctac caagcccgca gcttgaacca ccgttcccag catggctaaa
     7801 agaaaaatgg caatcacaag tgtgaaagcc actgtcattg ccactttacc tagacgtttc
     7861 agcgtccagt ttgattttat tctgtttact attgatggtt tcacatttac acctcttttc
     7921 gcacaggtgg tctggtatca aaggcatgga gcagttcttc aaatacaggg tggaactgta
     7981 tcacaccgac acgaccatat aaatcactga taaggcattg cccgttttcc aaatcacgca
     8041 atcgcttctg attgttttcg tcctctgggt ctacaccaaa aaaggctaag gtctttttaa
     8101 tctcgttaag gtcagtggaa cgaaatgcaa attttaagcc gaggttattt ttcagttttt
     8161 catctaagag gtcgtctgta ttttgggtca cgaaatatac cccagcgttc atagcacgac
     8221 cagcccgaac cagcttcata gatagtgttt ttccttgtgc tacctgtaaa aagctccatg
     8281 cttcgtctaa atctacaatc ttgaaaatgc ttcggtctgt atggataaag tctaaagcaa
     8341 aggtactaat gacaatcagc atagcaacgg ataaaagctc catagtggta tattcctcaa
     8401 aggaagtttc cttgtcggga agtaccaagt ccgcaacctg tataatgttc agttgttttt
     8461 ctaagctgat agactgctcc acatatccat cactaaagag cagatgtgca aagtcatagt
     8521 ctgtaaaact ttcgatatgg tcggctatgc tggtgcttat ttgagttccc tcaacccgta
     8581 attcctcaat cactttcaga agacctcgca cttcactatt ggttactgca cgaatggctt
     8641 ttcgtagaac agggaaacgg tcagaatctc gtgatgaaat ccccgtaagg aacgtcaaaa
     8701 tgtcgattgc cagtgattca gaatctttgg gatttttcat aatcacgtaa gggtcaagta
     8761 agcctttatt tttctcatca gaagtcagag tgacgatatt gatttcatgg gaaatctctg
     8821 gcaaggtttc tttccatctg ccacgttctg cttttgggtc tacaatcact gcttgtgccc
     8881 cataaagcac tgcataatag acgataaggt tattcgcaaa ggattttcca ccgcctagcg
     8941 aacctacaaa ggcagacgct aatgcattgg ttactgaacc cttaacccct tgactggcaa
     9001 gagcaggttt gaggtagaca ttgcgtcctg tatctaagct atagccaaca taaataccct
     9061 cattttcccc cagcatttga gtagcaccaa aacctaaacc agcgaggaaa tcagaggtca
     9121 cgtattgaat ataatcattc atataacgct tgctggcagg taaaaattct tcatgtaagc
     9181 cgagcatatc cccaaatggt cgtaccagtt ttacgcttaa atcgtcataa aaatctttca
     9241 cttcattaca acgacgtttg agttcgtcaa gatcatttgc tgataccctt accacataag
     9301 acagcttgta catagattcc ttgctttggt ctaaattggt ttccagctca ttcacacttt
     9361 ccagagcttc cgccacattg gagctggttt cattatcact ttgccaagcg tggttatcca
     9421 agtctttcag ttctttcttt ttattgcgga cagtagatag ggctttacga ttcgctacaa
     9481 tttccacatt cattgacgta tcaatcggga atgtaaattg ctgttgctgg tagtagaaga
     9541 tttcagagga cgggaagtcc agttctccga caatgctgtt aatggtaaag taagctacat
     9601 agacggtttc atcttcctgc tggattttca aatatcgctg tttttcttcc accaaacagc
     9661 gagtaggctt aatcaagtca tagtatttaa tcagcgtttc attatccagc tttttctttg
     9721 atagatggta ctcatactct tcataggcag tgcctgtctg tccgtaaagg tgttcaatca
     9781 gatagccgaa gtcgtcctta tctaacctgc ggattttgaa acgacgagag attttatttt
     9841 ctaagagctt ttccatcttc tgaaaacgca ggatttcatc attactcata ctaacaaaat
     9901 cgcccatcag cttatggttc acatcataga caaaatcaga caaagcattt tttgcttcaa
     9961 cggtaagact tttcatagaa aactcctgat cgttgagaag caacttaaag ccgataaaga
    10021 aacggtagtt cacttgattt tcgccaatca tggatattaa agcgtctgtc tgttggtcga
    10081 ttttgtcata ggcaaccgct ttgagcttgc cagtgacttc atttttggaa cgctcttgtg
    10141 cagaacgtat gctggattct gtactgattt gtaaagcatg aattttgcca tcacgatttt
    10201 gtgcgataag ctgtctgaaa gaatcatgca cttgtatttt ctgttctgga cttagaaatg
    10261 agtaattgta aggaacaagc tcatagtaag cataacattc cccgtcttta ttccagacga
    10321 gattgttttc aatgtattta attggatatg ccataaaatt cactcctaac tgctgtaatg
    10381 gcttcttgtg gctggtttct gccaagcgtt actttttttc ctgcataggt cagctttggt
    10441 cgcagtgcat aagcaatgac agacttcaaa aatccataag gctttttacc atcaaaagtt
    10501 tttgtagaca taaaccatgt gaaagccaca ggaatcccaa agtatttgag aaatgctccc
    10561 tctatcatgg aaagaggggg caagttgcca agtatcatca ctgcaaagag tgacacgaca
    10621 aaccatgtca tttgcgtaaa ggttatggga aacggaagtc taaaatcatt gatagaatac
    10681 agtacctttt ccacagacca gatactggta tagcttcgta ttttcttcat gtaatcaatc
    10741 ctttcataaa aaataggggt agctgattga gccaccccgt aaaatagaaa atctgccagt
    10801 agtaatgtac cgacagattt aatagacgat ttcaaaaatc ccatgattgg ttgagataaa
    10861 cgttcctgaa aggtctaaat cccgaccata ggcttgataa tcaatatagt tttgaagact
    10921 agctggtact tcgcctaaag cacccgtttc ttcaatgtag tagcgtgcca cgtcatacat
    10981 atcatcacaa tcggaatgaa tgataatatc ctcttgatgt tcgcttagtt cttcaatgct
    11041 tgaaaaatga gtgagcagag cagatagctc cgattgtaat tcttcgggta attccgatac
    11101 catttcccat agtcgattga gttcgccaat ggaagtgtat tcgtcaaccg taaagggtaa
    11161 ctcgtagtca tgaatggcgt attcctcata ttcatcattc aagccgattt tctctttgac
    11221 ttcctcaaag tcaatgggaa aggtaaacca cgcaccgacc aattcgccct cattgtattt
    11281 gcctaaattc gcaatataga cttgcatatc gtccatatat tcacgtcctt tctttgtaga
    11341 gattcaaaaa tccctaccgc acttcgtttg gtgtaccatt cctttgcgga acataagaaa
    11401 accacttata ttccacaaaa gaacggtttt atttaagcac caataatgcg attgaatagc
    11461 tctagtaaaa tgtcttttac tccagcagcg ttgaagacta agccaaccgc aataatcgca
    11521 ataattaaaa agccaatcag tttgctaaac tcacgcttga agccaagata caagccaatc
    11581 acaacgattg ctaaaagcac cagtgattga gcgtttgata gaaaccagtt ataaaggttt
    11641 tgtccaaaat tcataaaaat gttctcctct ctatattcaa tgaatttgta tttgagttat
    11701 ttttttgttg ttatcacgtc ctgttctttt actgactgtt gcttcaaaat ctgcttgtgt
    11761 cggtctgtca gtttcgcatg gtcgagaatg tcttttacaa cctgcgtctg gttgatttca
    11821 tcaagtttaa tcgcaacctt taaggtcggg gcaacttgat gagatagcca gttcagcgtc
    11881 ctttggaagg agtaaggctc tggttttgtg gttagtttta atcgttcacg attgttccca
    11941 ataaaccaag cccattcttc attcagtttc caatcagaac gaggtttgga atcgtcttta
    12001 tctacaaaac ggatataccg attgataatt ttaaaggcgg tatgctctgg attgtcatag
    12061 acgagtaaat cacggactgc ataataggca cgctcatttt tcaatcgaat ctcaaaacgg
    12121 ttttttactt ctgcgtcttc aatgggaata tcatttttct tgtactgctc gtagtccttt
    12181 tcatagatac agaaataaac ttcactttgt aatgaaccga tatagagggt gtttcccata
    12241 cattcctttt cctctttgcg taccagttcg ccactgcgat agcttttaaa actgcggaag
    12301 acggagatac attcttcctg ttggcacttt tcagtgagta cagggatatt caaaatccct
    12361 gtcttatcgt taatggcaag gtcaaggcgt ttcatcacac cgccagccac caaaacgtcc
    12421 ataaagaact cataccagct tctttgttgt gccagaagat agctttcaaa ttgtctgcac
    12481 ccacgacctt tcaattccac cagaactcct ttgtccagtt catgggagca aaggacgaat
    12541 atgtcgccta aagcataatg ctctgaataa gaatagaaac catagtcctc atgaagaaaa
    12601 taggacagtt tcagttgtaa gatgttttcg accacctgct gtacgtctgt tgtcggaaag
    12661 cgaatcctta cataatcaaa cagcatttca aggggagcgt cgggattgaa gcgttccaga
    12721 gcttcccaaa gggactgctg taaatcctct gatggcttga cttttcctgt ttcaatatcg
    12781 cttagatact gccttgtaat accagtcgca acagctaaac ggttttgaga tagtccataa
    12841 gccaagcgtt tttcttttaa atgctgtaac caagtttgtt cattcagtaa aaatccctcc
    12901 aatcaaaaag gcgtatgtca acttttaaag cccatttgac atacgctgaa attttgtaaa
    12961 tcccttgtaa ccaaaggatt ttctaatgtt tttttgactg tttcctgtcg atttgtaccc
    13021 ccctgttaga tacggggggt taagtgctgg cgtggctatt gccacaccag ccagcaagat
    13081 cagtccacac ctgcgacttc cgcttcgcac gtcgcctgcg tggactgtct gctgttggat
    13141 aactttttaa tttcctccaa gaaatcatat ccttttggta caaggggagt ataaaactct
    13201 gatatgacac ttgttcctac atcaacatag ccacgacctt tgattcgctt taagaagaaa
    13261 tccttttgta cgtcactgcc aaacatcatg ccatagccca tttcagacat acgacctaaa
    13321 gccactctga aattaaactg atcacggatt ccgtcgccta aatattttgc gtctggacgt
    13381 tgacaagcca gtattagaaa gaagccagct tgacgaccta acatgacaat ctgtttcagc
    13441 ttattcataa ctgcggtgtt ttcttttgtt cccagcattt ccatgaaagc gacgtattca
    13501 tcaaagatta agaagtgtgc cgggagacct aagtaagcat aatttttgcc agtcttatag
    13561 ttcttcatct gcttcatttc ctcactacgt ttcatcattt cttcatagaa tgtttcaatg
    13621 caagaaagca agtcttcttt tctatagtag acatttgcca tcacagaacc taagtccgca
    13681 aggtcagcat ttttcgggtc aagaatatac agttttgaat ctgtatgaag caaggcttca
    13741 atcagtgtca gtataaagta agttttaccg ccacctgtac caccagcaat caacatatga
    13801 gggagcttat catattccca ccatacgttt ttcattaagc gaagtttacc atctttagct
    13861 tctacttcat caatagaaat acgactggct atggtgtcat agagcaaagt atattccaca
    13921 taggaatcct ttaactcttt atccgtcagc tcacagtaca agccactctc taatttcttt
    13981 tccaagtgta agagttggtc ttgatatttt cccagcgtga tttccacccg tatctgtatc
    14041 aagccatttt taagtcgata atacatttta gggaagtagg ttatcttttc ctttgtacga
    14101 ccagcactat ctttaaagaa accctctgtt ttgacctgtt cagattcata ccacttgttt
    14161 tcaagtatca tctttgccag tttttgacgg tggtaaagtt gtttaaccgt atcatagcga
    14221 acccgtttga atacaaacgc taccagcaag cagataagaa ttgcgacact gaaactgata
    14281 attaaatagg gaatgtcaat cttatctgct tgtgataggt taaaatcctg ccagttgatc
    14341 tgctggattg tcttcacatg aaacagtccg acaaccagca ggaaaacagg caggagtgac
    14401 gctatcgtaa aatgaaagac taaatcttta ccagatgggc gaatcctttt accacgctgt
    14461 ttcatgcgaa aaagtctcct ttctacctag cgactatttg tcttgtgtcg gttctttctt
    14521 tgcttgtggt tgagctttga atgaactaga atcctttgtc agcacaatat cgtctgcctt
    14581 gatataccag tcaacatctg ctccttgata ggtggcagta gcaacggtgt ccgcaatggg
    14641 attgataagt tccacccgtg cgttataatc aaactctttc aaaggcacgc tggcaggaat
    14701 acttacttga atcatgcgtc cttgtccttt ggattttaag tcataggtac gttccttgat
    14761 ttcatctgaa accgacccgt cttcattttg gattctcact tcacgacgta gagcagagaa
    14821 tttcaattct ccaaaagtcg tgtctttatc taatacaatg ccatttgcta atctcatcat
    14881 ttttcctctc tttctttatt cttttatcat gtcgtcagca tgtaaaaggt aatttgtaaa
    14941 accacgagtg ccgattttgt agccctctgc ggtaatacgt ggattgacta acttcacacg
    15001 ttcctcaaag ccgaaatgtt tttcgccagc ttcagcagga agcaccacca caatatcatc
    15061 tgctctttga acatcagaat agagattata gcttcttgat aagacagtta gccgtccgtt
    15121 gattcttcgc tgaacgactt tatcctcgcc agcaaattct aaattgccga atgttttttc
    15181 catgttggga atcacaaatt taagttccat atttttacct atcctttctt ttttattggc
    15241 tgaatgaatg tttgatggtc ttaaagagtg gggaacgacc ttttgattct tgattttttg
    15301 ttttcataag ttcacttcct ttcaaaatcg ggtaaaaaaa tagacacctc attttttgaa
    15361 gtgtctacct attaaatatt caaattttat tggaagtatc tttatatctt cacttttcaa
    15421 ggataaatcg tcgtatcaaa gctcattcat aagtagtaaa ttagtagtaa attgagtggt
    15481 tttgaccttg ataaagtgtg ataagtccag tttttatgcg gataactaga tttttatgct
    15541 atttttgata taaaaaatag ataataaaac cctgaaaata aaactttcag ggttggttgg
    15601 ggtgcttgag aaattagtcg attttctcga cgtagagttg tttggcaatg tccataccta
    15661 cttgtaaatc ctcttcttta ctgaggatag tgaaagtgtt gctgaagcca tcaaactgct
    15721 ggacttggag ctggtctccg atgtgcagtg agtgcttatc cagataatgg agaatatcaa
    15781 aactatcgtg cacacgagtc aggcggtagg caccagcttc cttgatgtca gctagtggga
    15841 ggttattgat ttcaactagg agttctccct tggcaggaat agttcctccg tgggggcagg
    15901 tcttggggaa tccaagtaat ttttctagtc tctccacgaa tagctcagag acagtatgtt
    15961 ccaatacctc agcttcctcg tgaatctggt cacttgtata gtctaaatga tgaactagaa
    16021 aaacttcaat caagcggtgc ttacgataga gctcagagac cagtttgaga ccgaggtcag
    16081 tcagtagata gccacattcc ttgtccttta ggatgagatt ttcacttttc attcgtttga
    16141 tcatttcagt tacggcaggg ggagagactt gcatgcgagc cgcaatttcc ttgttggtaa
    16201 tcttatgcag gtctatgcca atttcataaa tacattttag atagtcttct ttgtttgggg
    16261 tcattcgttt cctcttgtct aatatcttta tctagtatat caaaaaaagc tagatttctc
    16321 tagctgtgac tttttatgtt atacttattg caagagaaaa aacgaggaga tggatatgac
    16381 gaaaatagct cttctttcag atattcatgg aaataccacc gccttggagg ctgttttggc
    16441 agatgctcgg cagctaggag tggatgaata ctggcttttg ggagatattc tcatgccagg
    16501 gacagggcgt agaaggattt tggacttgtt ggatcaacta ccgattacgg ctagagtttt
    16561 gggaaactgg gaagacagtc tttggcatgg tgtccgtaag gaattggaca gtactcgccc
    16621 cagtcaacgc tatctcttgc gccagtgcca gtatgtttta gaggaaattt ccctagaaga
    16681 aattgaagtg ctccacaatc aacctcttca aattcatcgt cagtttgggg atttgacggt
    16741 gggaattagt catcatctgc ctgataagaa ctgggggcga gagttgattc atactggcaa
    16801 acaagaggat tttgaccgct tggtgactca tcctccctgt gatattgctg tttatggaca
    16861 tattcaccag cagttgcttc gttacggtac tggtgggcaa ttgattgtca atccgggttc
    16921 gattggccaa cctttctttt tggacgctca gctacggaag gacttgcggg cccagtatat
    16981 gattttggag tttgatgaca agggcctgat agatatggac ttccgacggg tagactacga
    17041 tgtggcagct gaattgcagc tggctaaaga cctgagactt ccctattttg aggtttacta
    17101 tgaaagtctg gtcaatggga tccaccatac tcatcatcag gaatttttga gagaattggc
    17161 tcagaaagag ggttgcgatc gggagttaga cgactggttg aaaagtggta acgattgaca
    17221 ttacaactaa aaagggtata ataatagaaa aagaagagta gaggcagggt agcctcgtaa
    17281 aaaggagaag aggatgcaaa ttccaagtag atttaccatt gcgactcata tgctgataat
    17341 cattgccctc gaggggaagg aaagcaaggt gaccagtgat tttctggctg ctagtgtcgg
    17401 ggtcaatcct gtcattatca gaaagatctt gtcccagttg aagaaggcag agctgatttc
    17461 agtagcgcgt ggaacgggcg gaacagagat tgtcaaggac cttaaggata ttagtctttt
    17521 agatgtttat caggcggtcg aatgtcttgg taagacaggt caactcttca gtttccatga
    17581 caatccgaat ccaaattgcc ctgtaggagc tcatattcat gatgttttgg atcaaaaatt
    17641 ggagagaatt cagttgacta tggaggcaga acttggtcag accagtctag aaaaagtcgt
    17701 ggccgatgca gagagtcaga tgaaggatta agagggataa atgccctctt tttttctttg
    17761 gataattatg ataaaatgta gctattaaag gagtaagaaa tgtatctcgt tataggaatt
    17821 gtattagcct ttatagtgtc attttggaag gataatagaa gtttgtggaa tccagtatta
    17881 tttttattat ccctcatttc aagttgtttt tatctatcct acctttttta taaaaatgga
    17941 tacgagaatg ttcagttagc tttttatgta tttgcctttg tcttacttcc ttttttgctt
    18001 tttctaagtg gcatcttttt aatctataat ggggttattt tgttgaagcg agagggacgt
    18061 tccaagcccc attatctctc aatgctcttt gatttttatt gactattaga ttctatttaa
    18121 aaagacttag aatgcacatg aaagtatgta ttctaggtct tttgttttgt atacatttaa
    18181 ttttcaagag tattatctat ttttccatac ctggtaacga gtatcaatca ataactgggt
    18241 aaggcgaccc aaggtttctt tttcttctgg agtgagggat tgagcttgga caaagagatg
    18301 atgaatgtat tcaagcgcct ttaaggaagc caagtcattg atagagctaa taccagcatc
    18361 aagaatagtg ccaaagaaga ggtcgaagta gagctgaagt tcttcgtcag ggactgtggt
    18421 cacccattct ttaaaggttg tgtctacttg ccggctatca ctgttggtct tatccagttg
    18481 aacgaagtgc ttgtcctcaa tctgccaact aaaggtatcg tgctgggcga tgccacccag
    18541 ggcagtactc tgaacgatga tttggtgagc aggaatttcc agcatcatac cgataatgga
    18601 accttgtgga atgaatatct tgcttctatc cattatcctt tgataacccg cagtctgtgt
    18661 caattcttga tggagaccag gtgcatcaaa tgtataaact gctgtgatct gattttgcaa
    18721 actttgctca atttggctag cagcatagat agcgagattt cctcccttgg aatgcccagc
    18781 cagaataacc ttttgcttag gatgatgggc aaaaaagttc tttaaatagc gaagggcgtg
    18841 cttttgagca ggaatttcct tcatataggt caggtggaaa tcttccttcc agccaatgat
    18901 actgtcatct gtcccacgaa agacaatcag ataggtatcg aggctgacac gataagtcat
    18961 agccgcaaat tgcttttgca gttcagggtc gatgtcgttg ataaaatggg agagtttgca
    19021 atttttgaag cgcttgtgtt gagccaattt atctaataat tgaaggcgat ttttgctagt
    19081 aagcatggtg ggctctcttg gaacctgagg tgctagatct aaaagacgtt gaggaagtgt
    19141 ggagaccaga ttatcaaagg agaggtaggt gatttctatt aaggttagaa tgtctaactc
    19201 attcaaggga aggtcgtaat aagaatcata tgcgacatct ttcagatagt caaaaatatt
    19261 ggccataaga gctcctttct ttttatttat cttagcaaat ttccctcaaa ttagctagta
    19321 gcattgcctg tttgtactgg ctaaaaacag gctatttcaa attcagtttc agaccatctg
    19381 gcatggaaaa atctgttata ataatggaaa aggagaagcg catgcacaag attttattaa
    19441 tagaagatga tcaggtcatt cgtcaacagg tcgggaaaat gctctctgaa tggggatttg
    19501 aagtggtcct ggtagaagac tttatggaag ttttgagtct atttgttcag tcggaacctc
    19561 atctggtcct catggatatt ggtttgccct tgtttaatgg ttatcactgg tgtcaggaaa
    19621 tccgcaagat ttccaaggta cctatcatgt ttctttcttc gagagaccag gctatggata
    19681 ttgtcatggc aatcaatatg ggggcggatg actttgtgac caagcctttt gaccagcagg
    19741 ttcttttagc taaggttcag ggcttgttgc gtcgttccta tgagtttggg cgtgatgaga
    19801 gtttgctgga atatgctggt gttatcctca ataccaaatc catggattta cattatcaag
    19861 ggcaagtctt gaatttgacc aagaatgaat tccagatttt acgcgtgtta tttgagcatg
    19921 caggcaacat cgtagcacgt gacgacctga tgcgggaact ttggaacagt gactttttca
    19981 ttgatgataa taccctctct gtcaatgtgg ctcgtttgcg taaaaagttg gaggagcagg
    20041 gattggtagg atttatcgag accaagaaag gaatagggta cggattgaag catgcttgat
    20101 tggaaacaat tttttctagc ctatctgcgc tcccgtagtc gtctttttat ctatctgctt
    20161 tctttggcat ttcttgtctt actctttcag tttttatttg ccagtctagg aatttacttc
    20221 ctctactttt tcttcttgtg ttgctttgta accatcttat ttttcacttg ggacatattg
    20281 gtggaaacgc aggtctatcg ccaggaactt ctctatggag agagggaagc caagtctcct
    20341 ttggaaatag ctttagcaga aaaattagaa gcgcgtgaga tggaactcta tcagcagagg
    20401 tcaaaagcag aaagaaaact gaccgatttg ctggattact ataccttgtg ggtccatcag
    20461 ataaagaccc ccattgcagc cagtcaactc ttagttgcag aagtggtcga ccgccaactg
    20521 aagcagcagc tagaacagga aattttcaaa atcgactcct ataccaacct agttttacag
    20581 tacctgcgtt tagaaagttt ccatgatgat ttggtcttaa agcaggttca aattgaggac
    20641 ttggtcaagg aaataattcg taaatatgct cttttcttta ttcaaaaagg cttaaatgtc
    20701 aatctacatg accttgataa agaaatcgtg acggataaaa agtggctgct agtggttatt
    20761 gagcaaatca tctcaaacag tctcaagtac accaaggaag gtggtctgga gatttatatg
    20821 gatgaccaag agctttgtat caaagatacg ggaatcggga taaaaaacag tgatgttctc
    20881 cgagtatttg aacgtggctt ttcaggatac aatggccgtt tgacccagca gtcctctgga
    20941 cttggccttt atctatctaa gaaaatttct gaagaactgg ggcatcagat tcgtatcgag
    21001 tctgaggtcg gaaaaggaac gacagtgcgg attcagtttg ctcaagtgaa cttagtcctt
    21061 gagtaaaaat agatgagaaa aagcctccag attggaggct tttatgctag actcttattt
    21121 ctcaacgcgt gatttgttgg cgatatcagc taggatagct tgaacaaaat tatcaactga
    21181 gacagtttgt gtttcttttt ggccgtagcg acgaacgttg actgtttcgc cttccatttc
    21241 tttgtctcca acaattaatt ggtaaggaat cttgctagtt tgtgaagcac ggatcttgaa
    21301 ctgcattttt tcattgcgct catctacgtc tgcacggaca ccgcggtcac ggagtttctt
    21361 ggctacttcc caagcgtagt ccacgtgttt ttcgttagat actgggataa gggttacttg
    21421 gtgtggtgcc agccatgttg ggaaggcacc cttgtagttc tcaatcaaga tagctgtgaa
    21481 gcgttccata gttgagataa ccccacggtg gatcatgact ggacggtgat cttcgccatc
    21541 agctccgatg tatttgaggt cgaagcgttc tggcaacaag aaatcaagtt ggatagtaga
    21601 aagggtttct tcttttccaa gggcagtttt aatctggata tccaattttg gtccgtagaa
    21661 ggctgcttca ccttcggctt caaagtagtc cacgcccatt tcatcaagag ctgcacgaag
    21721 catggtttgg gcattttccc acatctcatc gttatcaaag tacttatgag tatcttgagg
    21781 gtcacgaaga gagaggcgga agcggtagtc agtcaagttg aagtcttcat aaacatcgat
    21841 aatcaactga agggcacgtt ggaattcttc ttggatttgt tctggagtaa cgaataggtg
    21901 accgtcgttg agtgacattt cacgtacacg ttgaaggcca gtgagggcac cagatttttc
    21961 gtaacggtgc atcataccga tttcagcgat acggattggc aattcacggt aagagtgaac
    22021 atggtgtttg aaaacttgga tgtggtgcgg acagttcatt ggacgaagga caaattcttc
    22081 cccgtcaccc atgtccatgg ttgggaacat gtcttcttgg taatgatccc agtgaccaga
    22141 agtcttgtaa agctcaacag aagcaagtgg tggagtgtag acgtgttggt agccagaagc
    22201 caactctttg tttacgatgt agcgttccaa ttcacgacgg atagtcgcac catttggcaa
    22261 ccagaatggc aaaccttgtc ccacttcttg tgaaatcata aagaggtcaa gctctttacc
    22321 aagtttacgg tggtcacgtt ccttagcttc ttcacgcatt tgaaggtagt ttttcaagtc
    22381 tttcttgtca aaccaagctg taccgtagat acgttgcatc atagcattgt cgctgtttcc
    22441 acgccagtac gcaccagcta catggagaag gtggaagatt tggatacgac ctgttgatgg
    22501 aacgtgaggt ccacggcaga ggtctacata ttcaccctga cgatagatag tcaaaccgcc
    22561 ttcgtcttct gagtgttctt caatcaattc caacttgtaa gggtcatttt tgaagatttt
    22621 acgtgcctcg tctttagtca cttcttcacg aatagatggg aagttttctt tgacgatttt
    22681 ttgcatttct tcttcgatac gaggaaggtc ttcgttagag atttgaccag ctgtattgtc
    22741 agtatcgtag tagaaaccat cttcgatggc tggaccaact cccaagtgaa tgtctgggaa
    22801 aagacgacga gctgcttggg cgaacaagtg agctgctgag tgacgcaaga ttggaagggc
    22861 atcttcgtga tcaggtgtca caatttcgat gcttccatct tcagtgatag cgcgagtagt
    22921 gtcgatgagt ttgccgttga atttaccagc caaggctttt ttagctaggg aattgctgat
    22981 agattgggca atttcaaaag ttgttacgcc agattcgaat tcacgaacag cgccatctgg
    23041 gaaagtaatg ttaatcatga tgttctcctt ttttattatt tcatagtttc taagcattga
    23101 ttttttatca gcagactact tccaagtcct aacgttcaat tgcttttctt tatcagagag
    23161 gaagttttcc atttccgtca gaatatctat atccaaacgt tgagaaagtt ctaatagtca
    23221 ccagatattt tctgaaagtt tttgttccag tgtgtagggt gtttcatcat agtagcgtcc
    23281 ttgttttgtc atcaccagtc gttggaaatt tccaatatca ttagataaag ccaagaggtc
    23341 ttcttctacc gtccacttgg aatcatgatg cttaacttcc agttcgttat aagcttgtcg
    23401 gattgcccaa ctgcgctcca ctaattcttg caactccata attagtatcc taataaaaaa
    23461 atgcgacatc ctcgaaagga cgtcgcaacg tggttccacc ttcatttatg tacctcaaaa
    23521 gagagggaca cctctgattg gctctaacgt ggccaccgtt ttatgttttc ataaaaaact
    23581 caagagtagt atcagtttag acttcctatg cgtttccagc aaccacgcac tctctagtag
    23641 aaagggacta agtgacttgt ctctatggat tattatagca tgattgtgag atttttcaag
    23701 gattttgtga aagttttttg tttttcattt tttgtagaat atgcttgatg ctaataccag
    23761 aatccatgcc caccagagca actgtttcat agattagcaa gtaaattagt ataaactcac
    23821 caaaggttat gatagtaaag taaaagagca gtccgttacc aactaaccat caaagtgaga
    23881 aacggaaacg gtagctatag agtaacgata caatcaagat taccagaggt gtaaagaaga
    23941 gaagattgtt tatatagtgt aagggggtca gaaacaacta gcaagaacaa ctcataaagt
    24001 ggccaataaa agaataatat gaccagatag tcggggagat gggaatagaa tctacgcatg
    24061 ataatcacct cgttttaaaa aaacagacaa cttagatctc ttgctatgat tattatagca
    24121 tatttaaaac aagaaagtaa atttgttgac aaagaagatt ttttttcact agaatcatgg
    24181 ttagttttct tggtagaaat attttgataa taattaagca agactattga cattgattat
    24241 taagagtgtc acaattgctg taactaaaat aattacaaca agagaggtat aaaaaatgaa
    24301 actagcagtt attgcagcaa atggacaagc ggggaaagca attgttgaag aagcagtcaa
    24361 acgaggacat gaagtaacgg ctattgttcg ttctgaaaac aaaagtcaag ctgagtctat
    24421 tattaaaaaa gatttatttg agttgacaaa agatgatctt actggatttg atgctgttat
    24481 ttcagcgttt ggtgcttata caccagatac gctaccgctc catagcaaat caatcgaact
    24541 atttaatcag cttcttgctg gaacacagac acgttttcta gttgtagggg gagcaggaag
    24601 cctttatata gatgaaacaa aaacgacacg acttttagat acgcctgatt ttcctgaaga
    24661 atttaagcca ctagcaaaag ctcaagcgga tgaatttgat cttcttcgta caaaaaataa
    24721 tctcaactgg acttttgtca gtcctgctgt agattttatt cccgatggtg aaaaaacagg
    24781 aaactacatt ctagcaggag aaatttttac tactaatgaa aaaggaataa gccagattag
    24841 ttatgcagat tatgctattg gattggtaga tgaacttgaa aaaggtcatc atataaaaga
    24901 acgtatctct ttacttgaaa aataataact atgagagtat taatgtttga cagaaaattt
    24961 aaatcttggt aaaataggga ttgtcgtaaa gacaaataac ttcttcttgg ttacaggcat
    25021 gccaacctgt cactcggatg aagccaaata aaaaggagaa acatcatggc aatctcaaaa
    25081 gagaaaaaaa atgaaatcat tgcacaatat gcacgtcacg aaggtgatac aggttcagta
    25141 gaggttcaag ttgctgtcct tacttgggaa atcaaccacc ttaacgaaca catcaaacaa
    25201 cacaaaaaag accacgctac ttaccgtgga ttgatgaaaa aaatcggtcg ccgtcgtaac
    25261 ttgcttgcat acttgcgtaa aaacgacgtt aaccgttacc gtgagttgat caactctcta
    25321 ggacttcgtc gctaattcaa gatacaagtg cttaggccgt gttcaattga gcatatcttg
    25381 ataatgaagc tactctaaat tgggtagctt ttttgcgttg tcaccttacc tcgctatact
    25441 caagtataat ctttggttac ggttcctagc actgtaaggt aaaataaacc agcttttctc
    25501 ccttcgggga gatttttttt gtttcactga aagtttcgta cgatcttcgc ctcgtcgcct
    25561 agtctataaa cgatttatct agcaagaatc atgatgctaa gggcgtcaaa aatccgtatg
    25621 aaaataggga aatgacacag tgttcgatga gcacaaggag tttcatcttt ttcactagga
    25681 ttttagcccg ggctcaaatc agctctctga cttcagaggg cttttttatt gaggttttat
    25741 cggtaacaat tttggagact acaaaaacct caaacggtat cagctaatta caccataaag
    25801 gtgcgcagtt actcggcttt tcaagccgag tagctgtatg caagccccct agaagagccc
    25861 actctttaca aagtaaagta tagtatgtta tactttacat ggaagtagtc actgaattcc
    25921 agttagaaat tactttgtaa ctacgttttg aggaggagta aaatgctttc ctacgttcga
    25981 cattacccac tagcgatagc taaattaatg tgtctgtgct ctcctaaaat ctgctgattt
    26041 attactgact aatacaggag ttttttttat gggacagaca atcatatctg ctattggtgt
    26101 ttatatttcc accagtatcg attatttaat tattttaatt attttatttg cacagctatc
    26161 acagaataaa cagaaatggc atatttatgc ggggcaatat ctaggcacag gcttacttgt
    26221 aggggcgagt ttagttgctg cttatgtcgt taatttcgtg cctgaagaat ggatggttgg
    26281 attgcttggt ttaatcccta tctatttagg gattcgcttt gcaattgttg gagaagatgc
    26341 ggaagaagaa gaggaagaaa ttattgaaag attagaacaa agcaaggcaa atcaactgtt
    26401 ttggacagtt acattgctga caattgcgtc tggcggagat aatttaggta tctatatacc
    26461 ttattttgct tcgttagatt ggtcacagac cctcgtggcc ttgcttgtgt ttgtaatcgg
    26521 cataattatc ttttgcgaga ttagtcgggt gttatcctct attccgttaa tattcgagac
    26581 aattgaaaaa tacgagcgaa tcattgtgcc cttagtattc attctacttg gactatacat
    26641 catgtatgaa aatggcacga tagagacttt tctgatcgtg tagatttttt tgtttcacta
    26701 ggattttagc ccgagctcaa atcagctctc tgattttcag agggcttttt cattgagtat
    26761 aaatggacaa tcttaaagtt actctattgt agagtggctt ttgatttgat taggagattc
    26821 ttataaatat agtaaaatga aacaaaaaca ggacaaatcg atcaggacag tcaaatcgat
    26881 ttctaaaaat gttttaagaa gtagaggtgt actattctag tttcaatttg atatataaat
    26941 gaaaacctta gctgtttggc caaggttcat ttcttctatg caagttcttc tcttttctta
    27001 tctgggttcc agatgaagct tgcgataaag ctaaagatga tggttaggat agcgaggata
    27061 atagcaagga tgagggcagg gatgcggata aaccaccaga gtggatttgg ttttttatca
    27121 ttgtgccatt gtttcgtttc ttcgtccaga cgttggaatt ctgtttgaat acgattggcg
    27181 actgtttcaa tgccttcatc attcattttc ttgatatctg agatatcgat tggatttcca
    27241 aagttcatat cgacacgttc acggctaatc aagcccttca aagtcatggg accggtgtag
    27301 gtaaccggca tgatacggac cttggccatt ttggcaatca gtgctgcgcc ccccttgaca
    27361 tcgtttgagt ggcggctacc acttggaaac atgatgagag agcggtcact ttttttgaga
    27421 acgttgatag gatatttgat ggctgaggcg ctgggatttt cacggtcgat gggaaaggcg
    27481 ccacacatac gaatccacca accaaagata cggttggtaa agagttcttt ttttgccata
    27541 aagatgaact gttttggctt ggtcgcaaag gccatataaa caggatccca ccaggtacgg
    27601 tgaggcgcaa ctaaaatata attttcatct tgattaggaa ttttatcagt attatgatag
    27661 tgagcattgc cattgatgga ccataagagc aatacaacta atccacgcaa ataagtataa
    27721 aacatgcgat ctccttcgat tgttttcttg ttattattat accttatcaa aggagggctg
    27781 gcaaactttt cccttgacta gatgcatatt tgggatgaaa ttagaattct gttaaaaaaa
    27841 atgatataat agaatttatg gataaaaata agattatggg attaacccaa agagaagtca
    27901 aggaaagaca ggctgagggt ttggtcaatg actttaccgc atcagccagt accagcactt
    27961 ggcaaatcgt taaacgaaat gtctttaccc tttttaacgc tttgaactta gccattgctt
    28021 tggcccttgc ctttgtgcag gcttggagca atctggtctt ctttgctgtt atctgcttta
    28081 acgctttttc tgggattgtg accgagctac gagccaaaca catggtggac aagctcaatc
    28141 tcatgaccaa ggaaaaggtc aaaaccatcc gtgatggtca ggaagttgct cttaatcctg
    28201 aagaattagt gctaggagat gtcattcgtt tgtctgcagg agagcagatt cctagtgatg
    28261 ccttggtttt ggaaggcttt gcggaagtca atgaagccat gttaacggga gaaagtgatt
    28321 tggtgcaaaa ggaagttgac ggcttacttt tgtcaggaag tttcctagcc agtgggtcag
    28381 ttttatctca agttcaccat gtcggtgcag acaactatgc tgccaaactc atgcttgagg
    28441 ctaagaccgt taaacccatc aactcccgta tcatgaaatc gctggacaag ttggctggtt
    28501 ttactgggaa gattatcatt ccctttggtc tggctctctt gctggaagcc ttgcttttaa
    28561 aaggcctgcc tctcaagtca tccgttgtaa actcgtcgac agctcttttg ggaatgttgc
    28621 ctaagggaat tgcccttttg accattactt cgctcttgac tgcagtgatt aagttgggct
    28681 tgaaaaaggt cttggtgcag gagatgtact ctgttgagac cttggcgcgc gtggatatgc
    28741 tctgtctgga caagacgggc accatcaccc aaggaaagat gcaggtggag gctgttcttc
    28801 cactgacgga aacttacggt gaagaggcta ttgccagcat cttgactagc tacatggccc
    28861 atagtgagga taagaatcca actgcccaag ccattcgcca gcgttttgtg ggagatgttg
    28921 cttatcctat gatttccaat cttcccttct ccagcgaccg caagtggggg gctatggagt
    28981 tagaaggctt ggggacagtt ttcttagggg cacctgagat gttgctggat tctgaagtcc
    29041 cagaagccag ggaggccttg gagagaggat cacgtgtctt ggtcttagct ctcagtcagg
    29101 agaaattaga ccatcacaaa ccacagaaac catctgatat tcaggctcta gccttgctgg
    29161 aaatcttgga ccccattcga gagggagcag cagagacgct ggactatctc cgttctcagg
    29221 aggtgggact caagattatc tctggtgaca atccagttac ggtgtccagc attgcccaga
    29281 aggctggttt tgcggactat cacagctatg tagattgctc aaaaatcacc gatgaggaat
    29341 tgatggccat ggcggaggag acagctattt tcggacgtgt ttcccctcat caaaagaaac
    29401 tcatcatcca aacgttgaaa aaagcgggtc atacaacggc tatgacaggg gacggggtta
    29461 atgatatctt ggcccttcgt gaggcggatt gttctatcgt gatggcggag ggagatccag
    29521 caacccgtca gattgccaat ctggttctct tgaactcaga ctttaatgat gttcctgaga
    29581 ttctcttcga gggtcgtcgc gtggtcaata acattgccca catcgccccg attttcttga
    29641 taaagaccat ctattccttc ctgttagcag tcatctgtat tgccagtgct ttactaggtc
    29701 ggtcagagtg gattttgatt ttccccttca ttccgatcca gattaccatg attgaccagt
    29761 ttgtggaagg tttcccacca ttcgttttga cttttgagcg aaatatcaaa cctgtcgagc
    29821 caaacttcct cagaagatcc atgcttcgtg ccctaccaag tgccctcatg gtcgtgttta
    29881 gcgttctttt tgtgaaaatc tttggaacga gtcaaggttg gtctgagtta gaaatctcaa
    29941 ctctcctcta ttatctcttg gggtcaattg gtttcttatc cgtatttaga gcctgcatgc
    30001 cattcactct atggcgagtc ctcttgattg tctggtcagt aggaggcttc ctagccacag
    30061 ctctcttccc aagaatccaa aaactgcttg aaatttcaac cttgacagaa caaacgttgc
    30121 ctgtttatgg tgtcatgatg ttggtcttta cagtgatttt catcctgact agtcgctatc
    30181 aagctagaaa ataaagaaag actgcaatct gtggattgca gtttttttag gtacaagatt
    30241 gccagccgaa atgtggtata ataagaggta atagagtttt ggaaagtgag agaagatgat
    30301 ttcaaagaga ttagaattgg tagcttcctt tgtgtcacag ggggctattt tactagatgt
    30361 gggaagtgac catgcttatc tgcctatcga gttggttgag agaggccaaa tcaaaagcgc
    30421 tattgcaggt gaggtggtgg aaggtcccta tcagtctgcg gttaaaaatg ttgaggctca
    30481 cggcctaaag gagaaaatcc aagtccgttt agccaatggc ttggcagctt ttgaagagac
    30541 tgaccaagtg tctgtcatta ccattgctgg catgggtggt cgtttgattg ctaggatttt
    30601 agaagaaggt ttggggaagt tagctaatgt agagcgtttg atcctccagc ccaataatcg
    30661 tgaagacgac ttgcgtatct ggctacagga tcatggattc cagattgtag cagaaagaat
    30721 cttagaagaa gctggaaagt tttatgagat tttggtggtg gaagcaggac aaatgaagct
    30781 atcagccagt gatgttcgct ttggtccctt cttgtccaaa gaagtcagtc cagtatttgt
    30841 ccaaaaatgg caaaaagaag ctgagaagct agagttcgcc ctcggacaaa tcccagaaaa
    30901 aaatctggaa gaacgtcaag ttctagtaga taagattcaa gctatcaagg aggtgctcca
    30961 tgttagcaag tgaagtgatc caagcttatg aagccttttg ccctcaggaa ttttctatgg
    31021 agggagacag ctgtggtctg caaattggca ctttagacaa gggtatccaa agggtcatgg
    31081 ttgccctcga tattcgtgaa gagacggtgg ctgaagccat tgaaaagggt gtagacttga
    31141 ttatcgtcaa gcacgcgcct atctttcgtc ctatcaagga cttgcttgcc agccgtccgc
    31201 aaaatcagat ttacatcgac ctgattaagc atgacatcgc agtttatgtc agccatacca
    31261 atattgatat cgttgaaaat gggctcaatg actggttttg tcagatgcta ggaatcgagg
    31321 agacgactta tctgcaggaa acaggtccag aacgtggaat tggacgtatt gggaatattc
    31381 agcctcagac attttgggaa ttggcccaac aggtcaagca agtctttgcc ctagatagcc
    31441 ttcgaatggt gcattatcaa gagaatgatt tgcagaagcc tatttcaaga gtagcaattt
    31501 gtggtggaag cgggcagtct ttctataagg atgctttggc aaagggggca gatgtctata
    31561 tcactggtga tatctactac catactgctc aggatatgct gtctgatggc ttgttagcat
    31621 tggatccagg tcactatatc gaagtgcttt ttgtggaaaa aatcgcagca ctccttactc
    31681 aatggaaaga gaaaaaaggc tgggaactag agattttacc tagtcaagca tcgaccaatc
    31741 ctttccgtca tatctagtta gaaagtgaag acaatgaaaa aagttgccat tattggagca
    31801 ggaattgtgg gagcaacagc tgcctactac ctctcgagag aaagtgacct agaggtgacc
    31861 gtttttgacc atgagcaagg tcaagccacc aaggccgcag caggaattat cagtccttgg
    31921 ttttccaaac gccgtaataa agcctggtac aagatggcgc gcttgggggc tgacttttat
    31981 gtggatttat tagctgattt agagaaatca ggacaaaaaa tcgactttta ccagcgttcg
    32041 ggagtctttc tcttgaaaaa ggatgaatcc aagttggaag aactttatca actggccctc
    32101 cagcgcagag aagaatctcc cttgataggg caattagcca ttctggacca agcttcagct
    32161 aatgaattat tccctggttt gcagggattt gaccgcctgc tctatgcttc tggtggagcg
    32221 agagtagatg gccaactctt ggtaactcgt ctgctagaag ccagtcatgt caagctggtc
    32281 aaagaaaaag tgactctgac actcttatca tcaggctacc agattggcga agaggtgttt
    32341 gatcaggtta ttttggcaac gggagcttgg ttgggggaca tgttagagcc tttaggttat
    32401 gaagtggatg tccgtcctca aaaaggacaa ctacgagatt atcagcttgc ccaagacatg
    32461 gaagattacc ctgttgtcat gccagaaggg gagtgggatt tgattccctt tgcagatggg
    32521 aaattatcct tgggcgctac ccatgaaaat gacatgggat ttgatttgac ggtagatgaa
    32581 cccttgctcc aacaaatgga ggaggcggcc ttgcctcatt acccagtttt agctagagcg
    32641 acttcaaggg ctgagcgtgt gggaatccgt gcctatacca gtgatttctc tcccttcttt
    32701 gggcaggtgc cagaattggc aggtgtctat gtagccagtg gactaggttc atcaggcctc
    32761 acaactggtc ctatcattgg ttaccatcta gcccaactga tccaagacaa ggagttgacc
    32821 ttggaccctg aaaattaccc aattgaaaac tatgtcaaac gagtaaaaag cgaataatac
    32881 tcaatgaaaa tcaaagaaca aactaggaaa ctagccgcgg gttactcaaa gcactgcttt
    32941 gaggttgtgt atagaactga cgaagttagc tcaaaacact gttttgaggt tgtgtataga
    33001 actgacgaag tcagctcaaa acactgtttt gaggttgtgt atagaactga cgaagtcagt
    33061 aaccataccc atggcaaggc gacgttgacg tggtttgaat tggattttcg aagagtataa
    33121 gcattttact gaaattttat caggtagttt aggatggcaa atgacattcc ctgtcaaaaa
    33181 tggtaaaata agaaaaaata atctgagaat cgaggaaaaa agatgcaaga aaagattttg
    33241 gtaactggtg gtgccggttt tattggaacc cacactgtta ttgaattgat ccaagcaggc
    33301 catcaagttg ttgtggtgga taatcttgtc aatagcaatc gtaagagttt agaagttgtt
    33361 gaaagaatca caggagttga gattcctttc tatgaggcag atattcgtga cacagttacc
    33421 ctcagagata ttttcaagca ggaagaaccg actggtgtca ttcactttgc tggtttgaag
    33481 gctgttggcg aatcaacacg tatccctctt gcctactatg acaacaatat cgctggaact
    33541 gtcagccttt tgaaagccat ggaagaaaac aactgtaaaa acatcatctt cagttcttct
    33601 gcgacagttt acggggatcc gcacacagtg cccatcttgg aagatttccc actttcagtg
    33661 accaacccat acggtcgtac caagctcatg ctagaggaaa ttttgaccga tatttacaaa
    33721 gcagactcag aatggaatgt tgtcttgctt cgttacttta acccaatcgg agcccatgag
    33781 agtggtgatt tgggagaaaa tccaaacggt attccaaaca atctcttgcc atatgtgact
    33841 caagtagccg ttggaaaatt agagcaagtg caagtgtttg gagacgatta cgatacggaa
    33901 gatggaacag gtgttcgtga ctatatccac gttgtcgatt tggctaaggg tcacgttgca
    33961 gctttgaaaa aaatccaaaa aggttcagga ctaaacgttt ataaccttgg aactggtaaa
    34021 ggttactcag ttcttgaaat tatccaaaac atggaaaaag cggtgggacg tcctattcct
    34081 taccgcatcg tagaacgtcg cccaggtgat atcgctgcct gctactcaga cccagcaaaa
    34141 gctaaagcag aactcggttg ggaagcagaa ctcgacatca cccaaatgtg tgaagacgca
    34201 tggcgttggc aaagcaagca tccaaatgga tttgaagact aagatgatga tttcaatcat
    34261 cgtcccttgt ttaaacgaag aggaagtact tcctcttttt tatcaggctc tggaagcttt
    34321 acttccagat ttggaaacag aaatcgagta tgtctttgtc gatgatggat caagtgatgg
    34381 gaccttggaa ctcttaaagg cctatcggga gcaaaatccg gcagtccatt atatttcttt
    34441 ctctcgaaat tttggcaaag aagcagccct ttatgcaggc ttgcaatatg cgacaggaga
    34501 tttggtggtg gtgatggatg cagacctcca agatcctcct agtatgttgt ttgagatgaa
    34561 aaatgtacta gacaaaaatg tagacttgga ctgcgttggg acacggagaa ctagtcggga
    34621 gggagaaccc ttctttcgca gtttctgtgc tgttctcttt tatcgcctca tgcaaaaaat
    34681 cagcccagta gctctgccgt cgggtgtccg tgattttcgt atgatgagaa ggtctgtggt
    34741 cgatgccatt ttaagcttga ctgagtccaa tcgtttttct aagggactct ttgcctgggt
    34801 cggctttaaa acccactatc tggactatcc aaatgtcgaa aggcaggctg gcaagaccag
    34861 ttggagtttt aggcaactct ttttttactc cattgaaggg attgttaatt tttcagattt
    34921 ccctttgact atagcctttg tagctggtct cctatcttgt tttctttctc tgctgatgac
    34981 cttttttgtt gtggttcgga ccctcatttt gggcaatccg acatctggtt ggacctctct
    35041 gatggctgtt attctctttc ttggaggcat tcaactcttg accattggga ttctcggtaa
    35101 gtatatcagt aagatttatt tagagactaa aaaaagacca ctttatctta tcaaagaaaa
    35161 aagtgacctt cctgatttta cagaaaaaat aaagtgaaaa gactataatt ttacatggaa
    35221 atgtgctaaa ctagagggag tggattatcg ccattgattt aggcttcctg gtttccaatc
    35281 agggcgcgag ttgggcaatt tttaacagcc tctaagacat cctgactagg agaaatttct
    35341 ttttccagtt ggtcagggtc atcgtaaaaa cgcacgattc cattatcgtg gtaatcaaat
    35401 aaatcagaat aagtttggca aagtccacag gcgatacatc gttcaggtat aagtgtgatt
    35461 ttcatgttca tatttatatt gtaataagaa aagtaaaaaa aaacaaggag taaggtatgg
    35521 caaaagaacc gtggcaagaa gatatttatg atcaagaaga atcaagagca gggcgtcggc
    35581 atcgaaacca cggaggggct gataggatgg ctaatcgtat tttgacgatc ctagctagta
    35641 ttttctttgt aattgtggtg gtgatggtca tcgttctcat ctatctatca tcggggggga
    35701 gtaatcgcac agcagcctta aaagactttc atgattctga tgcaagtgta gtacaaatct
    35761 catcttcaag cagttctcag gctgagcaga gttcagagcc agaatctact tctagtagtt
    35821 cagaagaagc tgctaatcct gaaggaacga ttaaagttct cgcaggagaa ggggaagcag
    35881 ctattgccgc tcgtgcagga atctccattg ctcagttaga ggccttgaat cctgggcaca
    35941 tggctacagg atcttggttt gctaatccag gtgatgttat aaaaataaaa taggagccga
    36001 tatgaaaaca attcaaattg ctattgatgg tcctgcttcc agcggtaaga gtacggtcgc
    36061 aaagattatt gctaaggatt ttgggttcac ctaccttgat acaggagcta tgtatcgtgc
    36121 agcgacctat atggctctta agaaccaatt aggagttgaa gaagtcgaag cccttctagc
    36181 cttgttggac caacatccaa tcagctttgg acgttcagaa actggagacc agcttgtttt
    36241 tgtaggagat gtggatatta cccatcctat ccgtgaaaat gaagtgacca atcatgtttc
    36301 tgctattgca gcaattcctg aagtgcgtga gaaactggtt tctctccaac aagaaattgc
    36361 ccagcaaggc gggattgtca tggatggtcg cgatattgga actgttgtat tgccacaagc
    36421 agaattgaaa attttcctag tagcttctgt tgatgagaga gcagagcgtc gttacaagga
    36481 aaatattgcc aagggaattg aaacagacct tgaaacccta aaaaaggaaa ttgctgcgcg
    36541 tgactacaag gatagtcatc gtgagacttc tcctctcaaa caagcagagg atgctgtcta
    36601 ccttgataca actggtttga acattcaaga agtagttgaa aaaatcaaag cagaagctga
    36661 aaaaagaatg taaatgtaga aaagccgata gattgatatc ggtttttttc taaatttgtc
    36721 aaaatgctga ttttagggta taatagttgc tgttcgagaa attttgattt tcaaagaata
    36781 gtgaatgatg ataaggagaa accatgaaca atttaccaaa ttgtccaaaa tgtaactcag
    36841 agtatgtcta cgaagacggt gccctactgg tttgcccaga gtgtgctcat gagtggaatc
    36901 ctgctgaagt tgcagaagta gaagaaggtc ttgtcgctat cgatgccaac ggaaataaat
    36961 tgactgatgg tgatacagta actctcatca aggacttgaa agtaaaaggt gcgccaaaag
    37021 atttgaaaca agggacgcgc gtgaaaaata tccgcatcgt agaaggcgat cacaatatcg
    37081 actgtaagat tgatggtttt ggtgccatga aacttaaatc agagtttgtg aggaagattt
    37141 aagcatgtca aaacactata aacttgtatt ttatagccgt atcttcttgt ttctagcggc
    37201 ttttacggga gtttatcttg aaattactag gcatggtggt tttgggatgc ttctctatta
    37261 cacggttctg tccaacctct tggtaactat ttttaccctt tatcttctaa aggttatgag
    37321 ccgtgtaggt gaaaattggc aaaggccaag tctcttgcgc ttaaaaggtg gggtcaccat
    37381 gagtatcatg attacctgtg tgatttacca tttcctcttg gcgcccattg cgactaattt
    37441 ctatacccta gaaaatttcc tttgccacta tatcgttccc atctggtttt tagcggatac
    37501 cctctttttt gacaaacagg gtcaatacaa gatttgggat ccagcagtgt ggaccatttt
    37561 accctttctg tatatgatgt ttgctctttt taatggcttg gttctaaaac tcaatattcc
    37621 aaatgccaag gataatcctt tcccttactt ctttttgaat gtgaacaagg gttggaatgt
    37681 tgtgtttaag tggtgtctga ttatctttgt tgcctatatg gtagcaggat ttattttcta
    37741 ctttatcaag caaatcaaga gaaagtcatc ctaatactct tcgaaaatct cttcaaacca
    37801 cgttagtttc catctgcaac ctcaaaacac tgttttgaac aacctgcggc tagctttcta
    37861 gtttgctctt tgattttcat tgagtatgag atacaaggga ttcattttcg aatttaagaa
    37921 ggagtctaca aaccaatgag gcttcttctt ttcttgctta tttgataact atcaaaaaag
    37981 tagaagatta agagaaatat agaaagagat ttcatgaaac aatttttaga acgggccagc
    38041 attttggctc tctccctcgt tttgattacc tccttttcca tttcgagtgc cctaccagcc
    38101 atgtttgact attatcaggg ttattctaag gaacaaattg agctcttggt gagcttgcct
    38161 tcctttggaa tcatgatgat gttactgcta aatggtttct tagaaaaaat atttcctgag
    38221 cgcttacaga ttagtttggg cttgctgatt ttatcattga gcggtacagc tcccttctgg
    38281 taccaagcct atccctttgt ctttggaaca cggcttctct ttggtttggg tcttgggatg
    38341 atcaatgcca aggccatttc tattatcagt gaacgctacc aaggaaaaag gcgaattcag
    38401 atgttagggc tacgcgcttc tgcagaggtc gttggagctt ctctcattac cttggccgtc
    38461 ggtcagttgt tggcctttgg ttggacagct atctttctag cctatagtgc tggatttttg
    38521 gtgctgcccc tttatctgct ctttgtccct tatggaaaat caaagaaaga agtcaagaaa
    38581 agagcgaagg aagcaagtcg tttaactcga gaaatgaaag gcttgatttt taccttagct
    38641 atcgaagcgg cagttgtagt ttgtaccaat acagctatta ccatccgtat tccaagtttg
    38701 atggtggaaa gaggattggg ggatgcccag ttatctagtt ttgttcttag tatcatgcag
    38761 ttgatcggga ttgtggctgg ggtgagtttt tctttcttga tttctatctt taaagagaaa
    38821 ctgctcctct ggtctggtat tacctttggc ttggggcaaa tcgtgattgc cttgtcttca
    38881 tccttgtggg tggtagtagc aggaagtgtt ctggctggat ttgcctatag tgtagtcttg
    38941 acgacggtct ttcaacttgt ctctgagcga attccagcta aactcctcaa tcaagcaact
    39001 tcatttgctg tattaggctg tagtttcgga gcctttacga ccccatttgt tctaggtgca
    39061 attggcttac taactcacaa tgggatgttg gtctttagta tcttaggagg ttggttgatt
    39121 gtaatctcta tctttgtcat gtacctactt cagaagagag cttaggattg attcctaagt
    39181 ttttcttttg atactcaatg aaaatcaaag agcatagttg attgagtttg gaatagtacg
    39241 ctgtagctgc taaaatattt ctagaaatta atttgacttt tctaatagag ttattcatat
    39301 cttatttcaa tctactatag aaagctagcc gcaggttgct caaaatagtg ttttgaggtt
    39361 gcagatggaa actgacgtgg tttgaagaga ttttcgaaga gtatgagatt tttataccca
    39421 tacaatattt gttttaggtc cttgtttaca gactaatttc ttgataaaat agaggttatg
    39481 acgagatata aagcaactat ttcctatgat ggttatgcct ttgctggctt tcagcgccag
    39541 tctcatgcgc gtagcgttca ggaagaaatt gaaaaaacct tgaccagatt aaataaaggg
    39601 caaaccatta ctgttcacgg tgctggtagg acagatagtg gggttcatgc cctgggacag
    39661 gtcattcatt ttgacctgcc ttatcagatg gatgaggaga aactccgttt tgccttggat
    39721 acccagtctc ctgaagatat tgatgtgatt tcgattgagc ttgtggcaga tgattttcat
    39781 tgccgttatg ccaaacatag caagacctat gagtttattg tggatagagg acgtcccaaa
    39841 aatcctatgc gccgtcacta tgccacccac tttccctacc cactcgatgt ggaacgaatg
    39901 cagattgcaa tcaaaaagct agagggaacc catgatttta ccggttttac agcctctggg
    39961 actagtgtag aggataaggt tcgcaccatc acagaagcta gtttaatagt cgatgagaca
    40021 ggacaatttt tgacctttac cttttcagga aatggtttct tgtataaaca gattcgcaat
    40081 atggtgggga ctcttctcaa aatcggtaac aaccgcatgc cagtagagca gattgatctc
    40141 atcttggaga agaaggacag gcaacttgca ggtcccactg cagcaccaaa tggtttgtat
    40201 ttaaaggaga ttcgttatga agaataatcg tattttagca ctttctggaa atgatatttt
    40261 tagtggtggt ggactgtcag ctgatttggc tacctatacc ttgaacggct tgcatgggtt
    40321 tgtagcagtg acttgtttga cagccttgac agaaaaagga tttgaagtct ttccaactga
    40381 tgataccatt tttcaacatg aattagatag cttacgtgat gtggaatttg ggggaattaa
    40441 gattggtctt ctccctactg tcagtgtggc tgagaaggcc ttggacttta tcaaacaacg
    40501 cccaggagta cctgtggtgt tggatcctgt cttggtctgc aaggaaacgc atgatgtagc
    40561 tgtcagtgag ctctgccaag agttgattcg ctttttccct tatgtcagtg tgattacgcc
    40621 taatctccca gaagcagaat tattatccgg tcaggaaatt aaaaccttgg aagacatgaa
    40681 aactgcagcg cagaaattgc atgatttagg agcgccagca gtcattatca agggaggcaa
    40741 tcgtcttagt caggacaagg ctgtggatgt cttttatgat ggacagacct ttactatcct
    40801 agaaaatcca gttatccaag gccaaaatgc tggtgcaggt tgtacctttg cctctagcat
    40861 tgccagtcac ttgattaaag gtgataaact tttgccagca gtagaaagct ctaaggcttt
    40921 cgtttatcgt gctattgcac aagcagatca gtatggagta agacaatatg aagcaaacaa
    40981 aaacaactaa aatcgccctt gtatccctat taaccgccct ttctgtggtt ctaggttatt
    41041 tcttaaaaat cccaacacct acaggaattc taactctttt agatgctggt gtcttctttg
    41101 cggcctttta ctttggtagt cgtgaaggag cggtagtcgg aggactagca agtttcttga
    41161 ttgacctctt atcaggctac cctcagtgga tgttctttag cttggtcaac catggcttgc
    41221 agggattttt cgcaggattt aaaggaaaaa gtcagtggtt aggccttatt ttagcaacta
    41281 ttgccatggt aggaggctac gccttgggtt ctactttgat gaatggctgg gcagcagccc
    41341 tcccagaaat tctaccaaat ttcatgcaaa atatggtagg gatgattgta ggatttattc
    41401 ttagtcaaag tatcaagaag attaagtaat actcttcgaa aatctcttca aaccacgtca
    41461 gcttccatct gcaacctcaa aacactgttt tgagcagcct gaggctagct ttctagtttg
    41521 cactttgatt ttcattgagt ataaaaagat cttgggaaaa gactcaattt cagtagaaaa
    41581 ggaagtaaat cttcccacat taaaacgcat attaccaagg ttcttatgaa ctgcacccca
    41641 aaagttagac agaaaaaatc taacttttgg gtgtttttat tatgaaattg aattatgatg
    41701 ataaagttca gatctatgaa cttagaaaac aaggatatag cttagag
//
LOCUS       DAVGJL010000005        38031 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.005, whole genome shotgun sequence.
ACCESSION   DAVGJL010000005 DAVGJL010000000
VERSION     DAVGJL010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 38031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38031
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.005"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            282..695
                     /gene="rpsL"
                     /locus_tag="VVE42_000210"
     CDS             282..695
                     /gene="rpsL"
                     /locus_tag="VVE42_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEU3072482.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            715..1185
                     /gene="rpsG"
                     /locus_tag="VVE42_000211"
     CDS             715..1185
                     /gene="rpsG"
                     /locus_tag="VVE42_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEU3072483.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            1610..3691
                     /gene="fusA"
                     /locus_tag="VVE42_000212"
     CDS             1610..3691
                     /gene="fusA"
                     /locus_tag="VVE42_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEU3072484.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEEIRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     AKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
     gene            3795..8186
                     /locus_tag="VVE42_000213"
     CDS             3795..8186
                     /locus_tag="VVE42_000213"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEU3072485.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFEIKARSQEFSNQLLQSYYREAFSEGP
                     CASQGFKSLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFPTFNCQ
                     VEKNDVLTQEQEEAFHAENEQIVQTANEEALRAMEQLEQMAPPPAEEKPVFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLTMNVQDLQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGMEANIVEDRVPIVYNEVEMDLSEATYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGHPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNATFDVGFMNANYERHDLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLARLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVPRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     AAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPVLATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFEPVEVVKGDLYTPFIDKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNKIPMDDEGVMALFSGTDVL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNGYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEVIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYRSQATEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
     gene            8288..8551
                     /locus_tag="VVE42_000214"
     CDS             8288..8551
                     /locus_tag="VVE42_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEU3072486.1"
                     /translation="MSKMSISIRLDSEVKEQAQQVFSNLGMDMTTAINIFLRQAIQYQ
                     GLPFDVRLDENRKLLQALTDLDQNRNMSQSFESVSDLMEDLRA"
     gene            8544..8822
                     /locus_tag="VVE42_000215"
     CDS             8544..8822
                     /locus_tag="VVE42_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HEU3072487.1"
                     /translation="MLKIRYHKQFKKDFKLAMKRGLKAELLEEVLNFLVQEKEHPARY
                     RDHSLTASKHFQGVRECHTQPDWLLVYKVDKSELILNLLRTGSHSDLF"
     gene            8843..8995
                     /locus_tag="VVE42_000216"
     CDS             8843..8995
                     /locus_tag="VVE42_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3072488.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLSGAIALAGIF
                     SALKEK"
     gene            9017..10258
                     /locus_tag="VVE42_000217"
     CDS             9017..10258
                     /locus_tag="VVE42_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEU3072489.1"
                     /translation="MVLPNFKENLEKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLHAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGALNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NAASDEEAVDFLWDQIFKTCRVYEADPVKAWEEHAAILKSKADMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAVNAQGEEFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGQIVDITAEKGDQVMKDLVFENAGARALGECALVPDPSPISQSGI
                     TFFNTLFDENASNHLAIGAAYATSVVDGAEMSEEELEAAGLNRSDVHVDFMIGSNQMD
                     IDGIREDGTRVPLFRNGNWAN"
     gene            10268..10498
                     /locus_tag="VVE42_000218"
     CDS             10268..10498
                     /locus_tag="VVE42_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU3072490.1"
                     /translation="MLGSMFVGLLVGFLAGAMTNRGERMGCFGKMFLGWIGAFLGHLL
                     FGTWGPVLSGTAIIPAILGAMIVLAIFWRRGS"
     gene            10766..11488
                     /locus_tag="VVE42_000219"
     CDS             10766..11488
                     /locus_tag="VVE42_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEU3072491.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVLDLLDDLARSKEVFPVGRL
                     DIDTHGLLLLTNDGQLAHVLLSPKRHVDKTYLAQVKGIMTQEDVETFAEGIPLKDFTC
                     QPAILELVSIDTEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRRLTKEELEILRANII"
     gene            11706..13040
                     /gene="pepC"
                     /locus_tag="VVE42_000220"
     CDS             11706..13040
                     /gene="pepC"
                     /locus_tag="VVE42_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEU3072492.1"
                     /translation="MNAIQESFTDKLFANYEANVKYQAIENAASHNGIFAALERRQSH
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQVTVQAKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGALAK"
     gene            complement(13096..14007)
                     /locus_tag="VVE42_000221"
     CDS             complement(13096..14007)
                     /locus_tag="VVE42_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU3072493.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(14031..14834)
                     /locus_tag="VVE42_000222"
     CDS             complement(14031..14834)
                     /locus_tag="VVE42_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEU3072494.1"
                     /translation="MSIISMVLVVVVAFFAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPAALL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            complement(14862..15851)
                     /gene="manX"
                     /locus_tag="VVE42_000223"
     CDS             complement(14862..15851)
                     /gene="manX"
                     /locus_tag="VVE42_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEU3072495.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQTAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
     gene            complement(16084..17103)
                     /gene="adhP"
                     /locus_tag="VVE42_000224"
     CDS             complement(16084..17103)
                     /gene="adhP"
                     /locus_tag="VVE42_000224"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEU3072496.1"
                     /translation="MKAVVVNPESTGVAIEEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKSLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWVVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLTLAKEVGADIVINGLEVE
                     DVAGLIKEKTDGGAHSAVVTAVSKVAFNQAVDSIRAGGRVVAVGLPSEMMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVVPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
     gene            complement(17442..18254)
                     /locus_tag="VVE42_000225"
     CDS             complement(17442..18254)
                     /locus_tag="VVE42_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU3072497.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKKTIKKVAEKGHQVIITTG
                     RPYRMSKDFYRELGLDTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVQRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPEDQFQPELVTKDPNCILLQTRASD
                     KYALAKDMNAFYQHQLSINTWGGPLNILECTPKGVNKAFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKTLQDLFL"
     gene            18444..19862
                     /locus_tag="VVE42_000226"
     CDS             18444..19862
                     /locus_tag="VVE42_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEU3072498.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTLMMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLIITLTNVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGIIL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFEDLKTIFGAALGSEGLGALVSDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNGENHQSAKLDKALYSDLIGTTVGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALVVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLGSLKNIYWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQVK
                     DVHAMIWILDALFILNYISMAL"
     gene            19915..20622
                     /locus_tag="VVE42_000227"
     CDS             19915..20622
                     /locus_tag="VVE42_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU3072499.1"
                     /translation="MKEKNMWKELLNRAGWILVFLLAVLLYQVPLVVTSILTLKEVAL
                     LQSGLIVTGISIVVLVLFIIGARKTQLASFNFSFFRTKDLARLGLSYLVIICSNILGS
                     ILLQLSNETTTANQSQINDMVQNSSLISSFFLLALLAPICEEILCRGLVPKKIFRGKE
                     NLGFVVGTIVFALLHQPSNLPSLLIYGGMSTVLSWTAYKTQRLEMSILLHMIVNGIAF
                     CLLALVVIMSRTLGISV"
     gene            20717..21691
                     /gene="folP"
                     /locus_tag="VVE42_000228"
     CDS             20717..21691
                     /gene="folP"
                     /locus_tag="VVE42_000228"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEU3072500.1"
                     /translation="MMKILEGVTDMSSKANHAKTVICGIINVTPDSFSDGGQFFALEQ
                     ALQQARKLIAEGASMLDIGGESTRPGSSYVEIEEEIQRVVPVIKAIRKESDVLISIDT
                     WKSQVAEAALAAGADLVNDITGLMGDEKMAHVVAKAGAKVVIMFNPVMARPQHPSSLI
                     FPHFGFGQTFTEKELADFETLPIEDLMVAFFERALARAAEAGIAPENILLDPGIGFGL
                     TKKENLLLLRDLDKLHQKGYPIFLGVSRKRFVINILEESGFEVNPETELGFRNRDTAS
                     AHVTSIAARQGVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK"
     gene            21693..23015
                     /locus_tag="VVE42_000229"
     CDS             21693..23015
                     /locus_tag="VVE42_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEU3072501.1"
                     /translation="MKEIENNQWIANYWTDQPHFGLERMVELLALRGNPHLKLKVLHI
                     GGTNGKGSTIAFLKKMLEKLGLRVGVFSSPYLIHYTDQISINGESISEARLEALMADY
                     QSLLEGEAVANLQGTTEFEIITALAYDYFASEQVDVAIMEVGMGGLLDSTNVCQPILT
                     GITTIGLDHVALLGDTLEAIAEQKAGIIKQGMPLVTGRIAPEALTVIDRIAEGKDAPR
                     LAYGTDYQVRHQESVVTGEVFDYTSAVRQGRFQTSLLGLYQIENAGMAIALLDTFCQE
                     DGRELASNDFLGQALEETSWPGRLEIVSRDPLMILDGAHNPHAIKALLVTLQERFADY
                     HKEILFTCIKTKALGDMLDLLGAMPDTELTLTHFADSRATDESVLKEAAKSRNLSYQD
                     WHDFLEQNLTDKKEEKQTVRIVTGSLYFLSQVRAYLMERKNENGYTKD"
     gene            22996..23550
                     /gene="folE"
                     /locus_tag="VVE42_000230"
     CDS             22996..23550
                     /gene="folE"
                     /locus_tag="VVE42_000230"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEU3072502.1"
                     /translation="MDTQKIEAAVKMIIEAVGENANREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            23593..24405
                     /gene="folK"
                     /locus_tag="VVE42_000231"
     CDS             23593..24405
                     /gene="folK"
                     /locus_tag="VVE42_000231"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEU3072503.1"
                     /translation="MDQLQIKDLEMFAYHGLFPSEKELGQKFVVSAILSYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFESYPLVQEMKLELKKPWA
                     PVHLSLDTCSVTIHRRKQRAFIALGSNMGDKQANLKQAIDKLRARGIYILKESSVLAT
                     EPWGGVEQDSFANQVVEVETWLPAQDLLETLLAIESELGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPILKQPIRNLYDALKK"
     gene            complement(24450..24767)
                     /locus_tag="VVE42_000232"
     CDS             complement(24450..24767)
                     /locus_tag="VVE42_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEU3072504.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMDGRETILLPEEN
                     DLF"
     gene            25124..25570
                     /gene="rplM"
                     /locus_tag="VVE42_000233"
     CDS             25124..25570
                     /gene="rplM"
                     /locus_tag="VVE42_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEU3072505.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISGLI"
     gene            25590..25982
                     /gene="rpsI"
                     /locus_tag="VVE42_000234"
     CDS             25590..25982
                     /gene="rpsI"
                     /locus_tag="VVE42_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEU3072506.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVVGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
     gene            complement(26244..26666)
                     /locus_tag="VVE42_000235"
     CDS             complement(26244..26666)
                     /locus_tag="VVE42_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU3072507.1"
                     /translation="MRFIVETFPIFSISNDLLQLLTSWKKQQKYELAKFGIISNPEQF
                     IFTYIDTKGNINKPLHSDYLNNKMKTIRKRHPELTHATPHKLRHTGATLAKQAGMSLE
                     AISEALIHSDTGTTQIYVNTSNVVPMAVGEFALQSLKQ"
     gene            26813..26989
                     /locus_tag="VVE42_000236"
     CDS             26813..26989
                     /locus_tag="VVE42_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3072508.1"
                     /translation="MSLDIDKEKMTIMGIAFENRSVFKSVWYALSTNMIEGWRPTVSD
                     VEKLRDEALALGMT"
     gene            27390..29603
                     /locus_tag="VVE42_000237"
     CDS             27390..29603
                     /locus_tag="VVE42_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HEU3072509.1"
                     /translation="MKIFKGEFYRISVLTDKLVRLEYSQTGSFEDRTTQLIYNRDFGQ
                     VSLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIELKGNFAVYGSRWYFGESIETLK
                     GTARTLDKADGAISLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
                     DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFATEKIPLSIGVL
                     DMDWHITDIPARFGSGWTGYSWNRNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYE
                     EAYPQIAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQGVDFWWIDWQQGTQ
                     GMLDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPY
                     FTATASNIGYSWWSHDIGGHILGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPW
                     FFSETTSKIMKKYLRLRHQMIPYLYTMNVKTHEEGAPLISPIYYFYPENDESYNVPNQ
                     YFFGTELMVAPIVEKMDLTFQSAKVDVWFPEGEWYDFFSEKKYTGGVKLSVYRDISTT
                     PVFAKSGAIIPLVGSEIGMGVDLPEVVDWYVFPGKQHSFEMLEDQNGQRYKTRLSIDW
                     EMGMVELALQGDSSIVPSNRKHRIHFKGTNVSIIELPNKNDTAKFEWKDNKRTSLNDE
                     VFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQDKELRGRLLEMIFTSQN"
     gene            complement(29825..30301)
                     /locus_tag="VVE42_000238"
     CDS             complement(29825..30301)
                     /locus_tag="VVE42_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEU3072510.1"
                     /translation="MTSLYDFSVLNQNNQATPLDSYRGKVLLIVNTATGCGLTPQYQG
                     LQELYERYQDQGFEILDFPCNQFMGQAPGSAEEINTFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLKDHKSGPLGKRIEWNFAKFLIGRDGQVFERFSSKTDPKQIEEAIQTLL"
     gene            30524..33728
                     /locus_tag="VVE42_000239"
                     /pseudo
     CDS             30524..33728
                     /locus_tag="VVE42_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
     gene            34076..34461
                     /gene="tnpA"
                     /locus_tag="VVE42_000240"
                     /pseudo
     CDS             34076..34461
                     /gene="tnpA"
                     /locus_tag="VVE42_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148688.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(34840..35469)
                     /locus_tag="VVE42_000241"
     CDS             complement(34840..35469)
                     /locus_tag="VVE42_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEU3072511.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNTKEEGLQASIACIKGGIKAIEI
                     AYTNQYAGQIIKELVDLYQDDQSVCIGAGTVLDAVTARDAILAGANYVVSPSFHAETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSTLSPAYISAVKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYITLR"
     gene            complement(35479..36480)
                     /locus_tag="VVE42_000242"
     CDS             complement(35479..36480)
                     /locus_tag="VVE42_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEU3072512.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSSICRLGDRIGLYYLENGFGCRQSEV
                     FYDRKHTSISQIRPNMLDMDSLFQGISHFHFSGITVAIGQEVRAILLLLLEEAKRRGI
                     VVSMDLNLRTKMISVLEAKYEFSKFARFTDYCFGIDPLMIDDQNLEMFPRDSASLEEV
                     ENRMRLLKEAYGFKAIFHTLRSSDEQDKNVYQAYALEERFEESVQLKTAVYQRIGSGD
                     AFISGALYQLLHHSSLKTTIDFAVASATLKCTLPGDHLSTSATSIENLLANAQDIIR"
     gene            complement(36511..37152)
                     /locus_tag="VVE42_000243"
     CDS             complement(36511..37152)
                     /locus_tag="VVE42_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEU3072513.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKGYQLFNYGMRGEEGE
                     SQLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFERLFAEEMGGGYPRERVIPEQRNARILNE
                     VKQITHNDLMTILKTIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK"
     gene            complement(37171..37986)
                     /locus_tag="VVE42_000244"
     CDS             complement(37171..37986)
                     /locus_tag="VVE42_000244"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEU3072514.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEAGATIVF
                     NDINQDLVNKGIEAYREVGIEAHGYVCDVTDEDGIQAMVKQIEQEVGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGGANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNPEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPE"
BASE COUNT        11386 a         7157 c         8555 g        10933 t
ORIGIN      
        1 gacggagata aaaatgtcac ataagccaag ccgtattatt aggaagatga tgtgttccca
       61 gctattttta ttcagaaatt gatctctaac attaccaata aaaaagaaaa atatcttgac
      121 aagcaaggga aaattttgtt acaataataa acggtacttt ttacttttgg tctctcaaga
      181 gtgtacaggg acgtgctgac aaatgttgca aaagtacaca cagatgatat ctgtcaccaa
      241 gtgtatcatc accaaaaata aaaaaacaca ggagaatgta gatgcctaca attaaccaat
      301 tggttcgcaa accgcgtaaa tcaaaagtag aaaaatctaa atcaccagct ttgaacgttg
      361 gttacaatag tcataaaaaa gttcaaacaa acgtttcttc accacaaaaa cgtggtgttg
      421 caactcgtgt tggaacaatg acacctaaaa aacctaactc agcccttcgt aaattcgctc
      481 gtgtacgttt gagcaacctt atcgaagtta ctgcctacat cccaggtatc ggacacaact
      541 tgcaagagca cagcgtggtg cttcttcgcg gtggacgtgt aaaagacctt ccaggggtac
      601 gttaccatat cgtccgtggt gcacttgata ctgcaggtgt taacgatcgt aaacaaggcc
      661 gttctaaata cggtactaaa cgtccaaaag cataaggaaa ggggataaag agaaatgagt
      721 cgtaaaaata gagctccaaa acgtgacgta ttgccagatc cgctatacaa ttcacaacta
      781 gttactcgtc ttatcaaccg cgttatgctt gatggtaaac gtggtactgc tgcttcaatc
      841 gtttacggtg cttttgagca aatcaaagaa gctactggca acgatgcact tgaagtattt
      901 gaaacagcta tggaaaacat catgcctgta cttgaagtac gtgcacgtcg tgttggtggt
      961 tctaactacc aagtcccagt tgaagttcgt ccagaacgtc gtacaacact tggacttcgt
     1021 tggttggtaa caatcgctcg tcttcgtggt gaacacacaa tgcaagaccg tcttgcaaaa
     1081 gaaatcttgg atgctgctaa caacactggt gcagcagtta agaaacgtga agatactcac
     1141 cgtatggctg aagctaaccg tgcattcgca cacttccgtt ggtaagatag gatgcgaaag
     1201 cgttaagaaa gtcccagaga aaatagggaa tcgaagcagg ttgcggttgc aaccaatgag
     1261 attcatcttt ttctccagac ttttagcttg agctcaacta aatcatgatg ctaggaacgg
     1321 taaggatgca aggtgaaaat aggaaactga cgcagtattc gacgaatata aggagtttta
     1381 tctttttcac gcagcatccc gttccagctc acatcggcta actaacttta gcccgggttc
     1441 aaattagcta aatcgattag tattagctat aactcagctt accatctcgt aagttgaaac
     1501 caacaatagc atgaaaacat tgagaacggg taggtcctgc ctatccgttt ttattaaaat
     1561 cgtgttataa tagaatagaa atcaaaaata aataggagaa acaaacctca tggcacgcga
     1621 attttcactt gaaaaaactc gtaatatcgg tatcatggct cacgtcgatg ccggtaaaac
     1681 aacaactact gagcgtattc tttactacac tggtaaaatc cacaaaatcg gtgaaactca
     1741 cgaaggtgcg tcacaaatgg actggatgga gcaagagcaa gaacgtggta tcacgatcac
     1801 atctgctgcg acaacagctc aatggaacaa ccaccgcgta aacatcatcg acacaccagg
     1861 acacgtggac ttcacaatcg aagtacaacg ttctcttcgt gtattggatg gtgcggttac
     1921 cgttcttgac tcacaatcag gtgttgagcc tcaaactgaa acagtttggc gtcaagcaac
     1981 tgagtacgga gttccacgta tcgtatttgc caacaaaatg gacaaaatcg gtgctgactt
     2041 cctttactct gtaagcacac ttcacgatcg tcttcaagca aatgcacacc caatccaatt
     2101 gccaatcggt tctgaagatg acttccgtgg tatcattgac ttgatcaaga tgaaagctga
     2161 aatctatact aacgaccttg gtacggatat ccttgaagaa gacatcccag ctgaatacct
     2221 tgaccaagct caagaatacc gtgaaaaatt gattgaagca gttgctgaaa ctgacgaaga
     2281 attgatgatg aaatacctcg aaggtgaaga aatcactaac gaagaattga aagctggtat
     2341 ccgtaaagcg actatcaacg ttgaattctt cccagtattg tgtggttcag ccttcaaaaa
     2401 caaaggtgtt caattgatgc ttgatgcggt tatcgactac cttccaagcc cacttgacat
     2461 cccagcaatc aaaggtatta acccagatac agacgctgaa gaaattcgtc cagcatctga
     2521 cgaagagcca tttgcagctc ttgccttcaa gatcatgact gacccattcg taggtcgttt
     2581 gacattcttc cgtgtttact caggtgttct tcaatcaggt tcatacgtat tgaatacttc
     2641 taaaggtaaa cgtgaacgta tcggacgtat ccttcaaatg cacgctaaca gccgtcaaga
     2701 aatcgacact gtttactcag gtgatatcgc tgctgccgtt ggtttgaaag atactacaac
     2761 tggtgactca ttgacagatg aaaaagctaa aatcatcctt gagtcaatca acgttccaga
     2821 accagttatc caattgatgg ttgagccaaa atctaaagct gaccaagaca agatgggtat
     2881 cgcccttcaa aaattggctg aagaagatcc aacattccgc gttgaaacaa acgttgaaac
     2941 tggtgaaaca gttatctcag gtatgggtga acttcacctt gacgtccttg ttgatcgtat
     3001 gcgtcgtgag ttcaaagttg aagcgaacgt aggtgctcct caagtatctt accgtgaaac
     3061 attccgcgct tctactcaag cacgtggatt cttcaaacgt cagtctggtg gtaaaggtca
     3121 attcggtgat gtatggattg aatttactcc aaacgaagaa ggtaaaggat tcgaattcga
     3181 aaacgcaatc gtcggtggtg tggttcctcg tgaatttatc ccagcggttg aaaaaggttt
     3241 ggtagaatct atggctaacg gtgttcttgc aggttaccca atggttgacg ttaaagctaa
     3301 gctttatgat ggttcatatc acgatgtcga ctcatctgaa actgccttca agattgcggc
     3361 ttcactttcc cttaaagaag ctgctaaatc agcacaacca gctatccttg aaccaatgat
     3421 gcttgtaaca atcactgttc cagaagaaaa ccttggtgat gttatgggtc acgtaactgc
     3481 tcgtcgtgga cgtgtagatg gtatggaagc acacggtaac agccaaatcg ttcgtgctta
     3541 cgttccactt gctgaaatgt tcggttacgc aacagttctt cgttctgcat ctcaaggacg
     3601 tggtacattc atgatggtat ttgaccacta cgaagatgta cctaagtcag tacaagaaga
     3661 aattattaag aaaaataaag gtgaagacta atccgtcctc actctagaag gaagtcactt
     3721 agtggcttcc ttttgtcttt agaaaatacc tctaaatatg gtaaaatagt agaagaataa
     3781 tgtgaggaaa atgaatgtca aatagttttg aaattttgat gaatcaattg gggatgcctg
     3841 ctgaaatgag acaggctcct gctttagcac aggccaatat tgagcgagtt gtggttcata
     3901 aaattagtaa ggtatgggag tttcatttcg tattttctaa tattttaccg attgaaatct
     3961 ttttagaatt aaagaaaggt ttgagcgaag aattttctaa gacaggcaat aaagctgttt
     4021 ttgaaattaa ggctcggtct caagaatttt caaatcagct cttgcagtcc tactataggg
     4081 aggctttctc tgaaggtcca tgtgctagtc aaggttttaa gtccctttat caaaatttgc
     4141 aagttcgtgc tgagggtaat cagctattta ttgaaggatc tgaagcgatt gataaggaac
     4201 attttaagaa gaatcatctt cctaatttag ccaaacaact tgaaaagttt ggttttccaa
     4261 cttttaactg tcaagtcgag aagaatgatg tcctgaccca agagcaggaa gaggcctttc
     4321 atgctgaaaa tgagcagatt gttcaaactg ccaatgagga agcgctccgt gctatggaac
     4381 aactggagca gatggcacct cctccagcgg aagagaaacc agtctttgat tttcaagcga
     4441 aaaaagctgc agctaaaccc aagctggata aggcggagat tactcctatg atcgaagtga
     4501 cgacagagga aaatcgtctg gtatttgaag gggttgtttt tgatgtggag caaaaagtga
     4561 ctagaacagg tcgtgtttta atcaacttta aaatgacgga ctatacttca agtttttcta
     4621 tgcaaaagtg ggttaaaaac gaggaagagg cccagaagtt tgacctcatc aagaagaatt
     4681 cttggctccg agttcgaggg aatgtggaga tgaataactt cacacgcgat ttgactatga
     4741 acgtacagga tctgcaggaa gttgttcact atgagcggaa ggatttgatg ccagaaggtg
     4801 agcgtcgggt tgagtttcat gctcatacta acatgtcgac tatggatgct ttgccagagg
     4861 tcgaagagat tgttgcaaca gctgctaagt ggggacacaa ggcggttgct atcacagacc
     4921 atgggaatgt ccagtccttt ccacatggct ataaggcggc taagaaagcg ggaatccagc
     4981 tgatctatgg gatggaagcc aatatcgtgg aggaccgtgt ccctatcgtc tataacgaag
     5041 tggagatgga cttatcagaa gcaacctacg tggtctttga cgtggaaacg acgggacttt
     5101 cagctatcta taatgacttg attcaggttg cggcctctaa gatgtacaag gggaatgtta
     5161 ttgctgaatt tgatgaattt atcaatcctg ggcatccctt gtcagctttt actacagagt
     5221 taactggaat tacagatgat catgtcaaaa atgccaaacc actagaacaa gttttgcaag
     5281 aattccaaga attttgcaag gatacggtcc tagttgccca caatgctacc tttgacgttg
     5341 gctttatgaa tgctaattat gagcgtcatg atcttccaaa gattagtcag ccagttattg
     5401 atacgctgga gtttgctaga aacctctatc ctgagtataa acgccatggt ttggggcctt
     5461 tgaccaagcg ttttggtgtg gccttggaac atcaccacat ggccaactac gatgcggaag
     5521 cgactggtcg tctgcttttc atctttatca aagaggtagc agaaaaacat ggtgtgaccg
     5581 atttagctag actcaacatt gatctaatca gtccagattc ttacaaaaaa gctcggatca
     5641 agcatgcgac catctatgtc aagaatcagg taggtctaaa aaatatcttt aagctggttt
     5701 ccttgtctaa taccaagtat tttgaaggag tgccacggat tccgagaacg gttctagatg
     5761 cccatcgaga gggcttgatt ttaggttcag cctgttcaga gggtgaagtt tttgacgtgg
     5821 tcgtttctca aggtgtggat gcggcggttg aggtggccaa gtattatgac tttatcgagg
     5881 tcatgccacc ggctatctat gcgcccttga ttgccaaaga gcaggtcaag gatatggagg
     5941 aactccagac cattatcaag agtttgatag aggttggaga tcgccttggc aagcctgttc
     6001 tggctacggg aaatgttcac tatatcgaac cggaagaaga gatttatcgt gaaattatcg
     6061 tccgtagttt gggacagggt gcgatgatta accgaactat cggtcatggt gaacatgccc
     6121 aaccagcgcc acttccaaag gctcattttc gaacgactaa tgagatgttg gatgaatttg
     6181 cctttttggg agaggaactg gctcgtaaac tggttattga aaacaccaat gccttggcag
     6241 aaatatttga acccgttgaa gtcgttaagg gtgacttgta tacgcctttc atcgacaagg
     6301 ctgaagaaac agttgctgag ttgacctata agaaagcttt tgagatttat ggaaatccgc
     6361 tgccagatat tgttgatttg cggattgaaa aagaattaac atccatactg gggaatggat
     6421 ttgctgtgat ttatctggca tcgcagatgc tggtgcaacg ttctaatgaa cggggttatt
     6481 tggttggttc tcgtgggtct gtcggatcta gtttcgttgc gaccatgatt gggattacgg
     6541 aggtcaatcc tctctctcct cactatgtct gtggtcagtg tcagtacagt gagtttatca
     6601 cagatggttc gtacggttca ggatttgata tgccccataa ggactgtcca aactgtggtc
     6661 acaaactcag taaaaacgga caggatattc cgtttgagac cttccttggt tttgatgggg
     6721 ataaggttcc tgatattgac ttgaacttct cgggagaaga tcagcctagc gcccacttgg
     6781 atgtgcgtga tatctttggt gaagaatatg ccttccgtgc gggaacggtt ggtacggtag
     6841 ctgccaagac tgcctatgga tttgtcaagg gttacgagcg agattatggc aagttttatc
     6901 gtgatgcaga agtagaacgc ctcgctcaag gagcggcggg tgtcaagcgg acaacaggcc
     6961 aacacccggg gggaatcgtt gttattccga actacatgga tgtctacgat tttacgcctg
     7021 tccagtatcc agcagatgat gtcacggctg aatggcagac cactcacttt aacttccacg
     7081 atatcgatga gaacgtcctc aaactcgatg tactgggaca tgatgatccg accatgattc
     7141 gaaaacttca ggatttgtct ggtattgacc ctaataaaat tcctatggat gacgaaggcg
     7201 tgatggcact cttttctggg actgatgtac taggggtaac acctgaacaa attggaacgc
     7261 ctacgggtat gttggggatt ccagagtttg gaacaaattt cgtacgtgga atggtagacg
     7321 aaacccatcc gacaaccttt gcggaattac ttcagctgtc tggtctgtcc cacggtactg
     7381 acgtttggtt ggggaatgct caggatctga ttaagcaagg aatagcggac ctatcgactg
     7441 ttatcggttg tcgggacgac atcatggttt acctcatgca tgcgggtctg gaacctaaga
     7501 tggcctttac cattatggaa cgggtacgta agggtttgtg gctaaagatt tcagaagagg
     7561 agagaaatgg ctatatcgaa gccatgaagg ctaataaggt gccagagtgg tatatcgaat
     7621 cctgtgggaa aattaagtac atgttcccta aggcccatgc ggcagcctac gttatgatgg
     7681 ccttgcgtgt agcttacttc aaggttcacc atcctattta ttactactgt gcttacttct
     7741 ccattcgtgc taaggctttt gatatcaaga ccatgggtgc gggcttggag gtcatcaagc
     7801 gcagaatgga agaaatctct gaaaaacgga agaacaatga agcctctaat gtggaaatcg
     7861 atctctatac aactcttgag attgtcaatg agatgtggga acgaggtttc aagtttggta
     7921 aattagatct ctaccgtagt caggcgacag agttcctcat cgacggggat acccttatcc
     7981 caccatttgt agcaatggat ggtctgggag agaacgttgc caagcaactg gtgcgggcgc
     8041 gtgaagaggg agaattcctc tctaaaacag aactacgcaa gcgtggtgga ctctcatcaa
     8101 ccttggttga aaagatggat gagatgggta ttcttggaaa tatgccagag gataaccagt
     8161 tgagtttgtt tgatgagttg ttttaaaaaa ttgcttaata atctattaaa agaggctaac
     8221 gtatatccaa tagatttaca ttagctttct tttttgttaa aatagtctat ggaaagaggg
     8281 tgagagtatg tcaaagatga gtataagcat ccgtctggat agtgaggtta aggagcaggc
     8341 ccaacaggtg tttagtaatc tgggaatgga tatgacaaca gctattaata ttttccttcg
     8401 tcaggcaatt caatatcagg gattaccttt tgatgttaga ctagacgaaa atcggaagtt
     8461 gctccaagcg ttaacggatt tagaccaaaa tcgtaatatg agccagtctt ttgaatcagt
     8521 ctcagatttg atggaggact tacgtgctta agattcgtta tcataaacag tttaaaaaag
     8581 attttaagtt ggctatgaag cgtggtttga aggcagaatt attagaagaa gttttgaatt
     8641 ttctggttca agaaaaagaa catcctgcca gatatcgtga tcattcattg acggcatcca
     8701 agcattttca aggagttcgt gaatgccata cccagccaga ttggcttttg gtttataaag
     8761 tagacaagtc ggaattgatt ttaaatttgc tgaggacagg cagtcacagt gatttatttt
     8821 aatctatttt aagggggttc tcatgaaact aagaatattt gcggaagata agccggctaa
     8881 gaaggtattt gaatatcaat tagaacttgc tgatcgtaca attcttctat cgacagcact
     8941 cttgtcaggt gctattgctt tagcaggaat cttttctgct ttgaaagaaa aataaaaata
     9001 gaaaagagaa aacagaatgg ttttaccaaa ttttaaagaa aatctagaaa aatatgcgaa
     9061 attgttggtt gcgaacggaa ttaacgtgca acctggtcac actttggctc tctctattga
     9121 tgtggagcaa cgtgaattgg cacatctaat cgtgaaagaa gcttatgcct tgggtgcgca
     9181 tgaggtcatc gttcagtgga cagatgatgt gattaaccgt gagaaattcc tccatgcccc
     9241 gatggagcgt ttggacaatg tgccagaata caagattgct gagatgaact atcttttgga
     9301 gaacaaggct agccgtcttg gggttcgttc atctgatcca ggtgccttga acggagtgga
     9361 cgctgacaag ctttcagctt ctgctaaagc tatgggactt gccatgaagc ctatgcgtat
     9421 cgcaactcaa tctaacaagg ttagctggac tgtagcagcc gctgctggac ttgagtgggc
     9481 taagaaagtc ttcccaaatg ctgcgagcga cgaagaagca gttgatttcc tttgggatca
     9541 aatcttcaaa acttgtcgtg tctacgaagc agatcctgtt aaggcttggg aggaacatgc
     9601 agccattctc aagagcaagg ccgatatgct taataaggag caattttcag cccttcacta
     9661 cacagcgcca ggaacagatt taacacttgg tttgccaaag aaccacgttt gggaatcagc
     9721 tggtgctgtc aatgcacagg gcgaagaatt cttgccaaat atgccgacag aagaggtctt
     9781 cacagcgcct gacttccgtc gtgcagatgg ttatgtcact tctacaaaac cgcttagcta
     9841 caacggaaat atcattgaag gcattaaggt gacctttaag gatggacaaa tcgtagatat
     9901 cactgctgag aagggtgatc aggttatgaa agaccttgtc tttgaaaatg cgggtgcgcg
     9961 tgccttgggt gaatgtgcct tggtaccaga tccaagtcca atttctcagt caggcattac
    10021 cttttttaac acccttttcg atgaaaatgc gtcaaaccac ttggctatcg gtgcagccta
    10081 tgcgactagc gttgttgatg gagcggagat gagcgaagag gagcttgaag ctgcagggct
    10141 taaccgttca gatgttcacg tagactttat gattggttct aaccaaatgg atatcgatgg
    10201 tattcgtgag gatggaacgc gggtacctct tttccgtaat gggaattggg caaattaagg
    10261 agataatatg ttaggaagta tgttcgttgg tctcctagtg ggatttttag caggtgctat
    10321 gaccaatcgt ggagagcgaa tgggatgttt tggaaaaatg tttctcggtt ggatcggagc
    10381 ctttctaggt cacttgctct ttggaacttg ggggccagtt ttatcaggaa cagctattat
    10441 cccagcgatt ttaggagcca tgattgtttt agctattttt tggagacgag gaagttaatt
    10501 tcctaaatct gatactcaat gaaaatcaaa gagcaaacta ggaaactagc cgcaggttgc
    10561 tcaaagcact gctttgaggt tgcagataga actgacgaag tcagctaaaa acactgtttt
    10621 gaggttgcag atagaactga cgaagtcagt aacatatata cggcaaggcg acgttgacgc
    10681 ggtttgaaga gatttttgaa gagtatgaaa cgaaaaggag gttggtcatt gtactagcct
    10741 ccttttgata tgatataata gttctatgag attagataaa tttttagttg cctgtgcggt
    10801 gggaagtcgg actgaggtca aaaacttgct caaggctggg cgcgtgactg taaatggtaa
    10861 aaaagaaaaa tcagctaaat tgcagattga tgaaaaaata gatgagattc gctttgatgg
    10921 gcaagtgttg gagtatgaag agtttgtcta ctacatgatg aacaagccca aaggagttat
    10981 ctcagcgact gaggatccca agcacagaac cgttctggac ttgttggatg acttggcgcg
    11041 gagtaaggaa gttttcccag taggacgctt ggatattgac acgcatggtc ttttgctctt
    11101 gaccaatgat ggtcagctgg ctcatgttct tctttcgccc aagcgtcatg tggacaagac
    11161 ttatctggca caagtcaagg gaatcatgac ccaagaagat gtggagacat ttgctgaggg
    11221 tattcctctc aaagacttta cctgtcaacc cgctatactg gagcttgtat ccatagatac
    11281 agaaaagaat caaagccaaa tccgtgtgac cattgcagaa gggaagtttc atcagatcaa
    11341 gcgtatggtg ggctattgtg gcaaggaagt ggtggacttg caacgattga ctatgggaac
    11401 tttagtatta gatgagaacc tggaacgagg ggaatggcgt cgcttgacca aggaagaatt
    11461 agaaattctc cgcgctaata ttatttaatt tagtttaata ctcaatgaaa atcaaagagc
    11521 aaactaggaa actagccgca ggctgtactt gagtacggca aggcgacgtt gacgtggttt
    11581 gaatttgatt ttcgaagagt ataaattcga aaaaggtgag ggacaatgtc ctcgcctttt
    11641 atgtttttta gttgcttcct ttgtgaaaag agttataata gactgtagaa taaaaggagg
    11701 aatctatgaa cgcgattcaa gaatcattta ctgataaact atttgccaac tatgaagcaa
    11761 atgtaaaata ccaagcgatt gaaaatgctg ccagccacaa cggaattttt gcagctctgg
    11821 aacgtcgcca aagccatgta gacaacacac ctgttttctc attggattta accaaggaca
    11881 aggtcactaa ccagaaagcg tctggtcgtt gctggatgtt tgcggctctc aacaccttcc
    11941 gccacaaact catctcgcaa tacaaattgg agaactttga gttgtcacaa gcccacactt
    12001 tcttctggga taagtatgag aaatcaaact ggttcttgga gcaagtcatt gcgacttcag
    12061 accaagaatt gactagccgc aaggttagct tcttactcca aacacctcaa caagatggcg
    12121 gtcaatggga tatggtcgtt tccctctttg aaaaatacgg tgtcgtgcct aagtcagttt
    12181 atcctgagtc tgtttcatct agcagcagtc gtgagctaaa tgcgatcctt aataaattgc
    12241 ttcgtcaaga tgctcaaatc ttgcgtgatt tgcttgtttc tggtgcagat caagtgactg
    12301 ttcaagctaa gaaagaagac ctcttgcaag aaatctttaa ctttcttgct atgtcattag
    12361 gacttccacc acgcaagttt gactttgctt atcgcgataa agataacaac tacaaaagtg
    12421 aaaaaggaat cacaccacaa gagttctaca agaaatatgt caatcttcct ttagaagact
    12481 acgtttctgt tatcaatgct ccaactgctg ataaacctta cggaaaatct tacacagttg
    12541 agatgttggg gaatgtggtt ggtagccgtg cagttcgtta catcaacgtt ccaatggagc
    12601 gcttgaaaga attggcgatt gcccaaatgc aagcaggtga gactgtttgg tttggttctg
    12661 atgtcggcca gctcagtaac cgtaaggctg gaatccttgc gacagatgtt tatgactttg
    12721 aatcaagcat ggacattaaa cttactcaag acaaggctgg acgtttggac tatagcgaaa
    12781 gcttgatgac ccacgccatg gtcttgacag gtgttgactt ggacgaaaat ggtaaatcaa
    12841 ccaagtggaa ggttgaaaac tcatggggag acaaggtcgg tacagatggt tactttgttg
    12901 cctcagacgc ttggatggac gaatacacat accaaatcgt tgttcgtaag gaattgctga
    12961 cagcagaaga acaagctgcc tatggagcag aaccaattgt acttgcacca tgggatccaa
    13021 tgggagcctt ggctaaataa aagcatagaa aaaaggaatc agattttaga acctggttcc
    13081 tttttagttg cttgattaca tgatgtgaag aacatgtgcc acaataccca ctgcgaagag
    13141 ggcaaggata atagtgattg gagatacttt tttcttaagt aagtacatgc aaagtaaagt
    13201 aaggagtagt cctgataatc caggaatcaa catatccaag ttttgttgga aagtagtaac
    13261 tttttcagga gtttgagaca atccttgtcc tacttgtgcg aatgcttctt ggataccttt
    13321 agacccttct ggcaatttat cccaatggat ataagccttt tcatctagtt gaactttaga
    13381 aacatcgaaa gcaaatttaa tatttaccca gcgttgaaca aggacagcaa gaatgaacat
    13441 cccaaggata gaagctcctt tagtgatatc ttgaaggata ccaccagaca tatctttagt
    13501 gatttctgat ccagccttgt atccaatctc ttgaacatac cacaagaatg acatacgaat
    13561 caagttccat gcaacaaaga agaggagtgg ccccaagata ttgccagtaa gggcaagtga
    13621 agcaccgaga gatccaagga ttgggcgtac tgtaaaccag aatactgggt caccgatacc
    13681 agcaagaggt cccatcatac cgattttaac cccttggata gcagcgtcat cgatttccac
    13741 accgttagca cgttcttctt caagcgcaag agtaaccccc atgactggag cagctacgta
    13801 tggatgagtg ttgaagaact caaggtgacg ctcaagagca gcgatttgat cttctttttt
    13861 agtatagagt tttttgatag ctggaatgag tgtataagcc cagcccaagt tttgcatccg
    13921 ttcaaagttc caagaccctt gtaagaaggt tgaacgccac caaacttttt tacgatctga
    13981 tttagttaat tgaagttttt cagtcatgat gttttcagtc ctttcttatc ttagtagtct
    14041 tctaggatat cgccgattgg gtcgttagaa gttgcggctc ctccgccacc atttccacca
    14101 gttttagaaa ggtgaaggta gataagagcg atagcaacac cgatagcacc gaatccgatt
    14161 agagtaatat ctgacacagc agcgagaacg aaaccaagag cgaagaatgg ccatacttca
    14221 cgagttgcca tcatgttgat aaccatggcg taaccaacgg caacgaccat accaccaccg
    14281 atagccatac catctttgag ccagtctggc atggcactaa ggatactttg tacagtttca
    14341 gttggtacca taaggagaag agctgcagga agcgcgatac gaagtccttg gaagagtagc
    14401 gcaatgaaat gcgcacgctc cacagcgccg aagtcacctt ttttagcggc agcatctgca
    14461 gtatgaacca aaccaactga aattgtacga acaatcattg tcaagaaaag tccagctacg
    14521 gcaagaggga tagcaaccgc ttgggcaaca ccgataccag tcttggtaaa gtcaccacca
    14581 agaaccatga taatggcagc agcgacagaa gcaagtgcag catcaggagc gatagcagca
    14641 ccgatatttg accaaccaag ggcaatcatt tgaagcgatc caccgaggat aatccctgct
    14701 tccaagtgac ctgttacaag cccaataagg gtacaggcta caagtggttg gtgaaattgg
    14761 aactggtcga ggatgccttc aagacctgca aagaaggcta caacgactac taaaaccata
    14821 gaaataatag acatgtttaa aatcctttca taaataatgg cttatttgac attggctttg
    14881 ttaatcaagt caaacaaatc ttttttagaa tcatttggta ctttacggac atcaaattca
    14941 acacccaagt cacgcatttt ttcaaatgta gcaacatctt ctttgtccat agacaaaacg
    15001 gtattgacca atgttttacc tgttgagtga gccatagaac caacgttaag agtcttgatt
    15061 ggcacgccgc cttcgatggc acgaagggca tcttgaggtg tttcaaacaa gataagggca
    15121 tgtgtttctc caaaacgtgg gtcttttgaa atctcaatca gtttttgaat tggaaccacg
    15181 ttagccttga cattacctgg agctgcttgt ttaatcaatt ctttacgaag gtcgtcttta
    15241 gccacgttat ctgaagcaac gatgatacga tttgcttttg aatctggagt ccaagcagtt
    15301 gcaacctgac cgtgaagtag acgtgtgtca agacgggcaa gattgatttt caatttaccg
    15361 tctccgataa cagttccttc tgggatagca gtttgggcaa ctggagcagc tgcagcgctt
    15421 gcaacttctt caactggatt tagctcttct ggaagagctt tgatgccatc tttggcttct
    15481 ttaatgatat tagcagcgac tttttctaca cctgcagcag cgtccatgag gcgctctgtg
    15541 taggcttgaa tcaacatcgg taagttaagt cctgtgatga tggcaaactt acgctcagga
    15601 ttttctccca tcacgcgact agcttggtta aatggagaac cactccaaag gtcagccaaa
    15661 actagaacct catcttctgc gtcaaatgca gcaacagcgt tattaaactt agcgtataga
    15721 tcatcaggac cttcatttgg cataaaggtc acaacttgaa ccttttcttg ttcaccaaag
    15781 atcatagatc ctgactgatg aatacccgca gcaaattcgc cgtggctcgc aataatgatt
    15841 ccgatactca ttatcgtcat tcctcctttt ttgttttagt tttcatttaa gaaaacttta
    15901 agactgttta agtataaacc gttttcataa aaaaagcaac tatttatcat aaaaaattaa
    15961 aaaattttta tttttgtatg ttgatatgtt gagtttttga ggagtaatct tgaattctta
    16021 tttaaaattt aatataaaaa agttggaaga agcttccaac ttttaaaata ggtttctatt
    16081 aaattagtgg gtgaagtcga gtaccatacg tccttggatt tggccttttt ccatttcgtc
    16141 gaaaatggca acagcatctt ctactggacg tttttgaaca actgggacta ccagaccttc
    16201 tgcaccaaat tggaaggctt cttctaagtc tttacgagtt ccgacaagag aaccgatgac
    16261 ttggattcca tcgaggactg ttttaacgat gctgagttcc atcatttcag aaggaagacc
    16321 aacagcgacg acgcgaccac cagcacgaat ggagtcaaca gcctggttga aggcaacttt
    16381 agacacagca gttacgacag ctgaatgagc tcctccatca gttttttctt taatgagtcc
    16441 agctacatct tcaacttcga ggccgttaat cacaatgtca gcgcctactt cttttgcaag
    16501 ggtaagtttg tcattgttga tatcgactgc gataacatga gcattgaata cttttttagc
    16561 gtattgaaca gcgaggttac caagtccacc agcaccgtaa agaacaaccc attggcctgg
    16621 ttcaactttt gcttctttga tagctttata ggttgttact ccagcacatg tgatagaaga
    16681 agcttgggct ggatcaagtc cgtcaggaac tttgacagca tagtcagcag ttacgataca
    16741 ttgttcagcc ataccaccgt ctactgagta gccagcattt ttcactgtac ggcaaagggt
    16801 ttcgcgacca gttgtacagt attcgcaagt gccacatcct tcaaagaacc aagcaacgct
    16861 gacgcggtca ccgactttaa ggcttttcac atctggagca atctctttaa cgataccgat
    16921 accttcgtgc ccaagaacac gtcctgggac ttgaccaaag tcaccatgag caacgtggag
    16981 gtcggtgtgg caaacgccac agtattcaac ttctacaagt gcttccccag tttcaagtgg
    17041 acggagtact ttttcttcaa tagcaacacc agtgctttct ggatttacaa caacagcttt
    17101 catagctatc ctccttgata ttgaccgaga acagggacaa cgggactgga ttgatttttt
    17161 gtcaacagag tcgagtgtga cgagctctct tgtatttata tgtgaagtat atcacaaaag
    17221 aaagcctttt ccaaggtttt ggagacaaaa agtagaacaa tcgattaact ggttgatgag
    17281 actgtgaaaa gagcacaaag accagcttgc aagctggtca tactcttcga aaatctcttc
    17341 aaaccacgtc agcttcatct acaacctcaa agcagtgctt tgagcaacct acggctagct
    17401 tcctagtttg ctctttgatt ctcattgagt atcagtgtag gctataagaa taagtcttgt
    17461 agggttttgg caaccccatc ttggtcgttg gtaagggaaa tttgctcatc tgcataaggg
    17521 agtagctctg gattggcatt tttcatggca taacctttcc cagcaaaagc gagcatttcg
    17581 gtatcattgt gttcatctcc aaaggcaatc aaatcttttt tgtcacgatt cattatcttg
    17641 agcaagtagt ccaaagcaaa ggccttgttg acaccttttg gggtacattc aaggatattg
    17701 agcggacctc cccaggtatt gatagaaagt tgatgctggt agaaggcgtt catgtctttt
    17761 gccaaggcat atttgtcact ggctctggtc tgcaagagga tacagttagg gtccttggtc
    17821 accaattcag gctggaattg atcttcaggc tggaaagctt ctacaccaaa tagtttggga
    17881 ttggcaatct cttcattggg atttgtaatg tagaattttt tacgatattc tccagcgata
    17941 aaatcggctt gaatgtcctc tgaacgttga accatatcta gcagatattt tttgtctaca
    18001 gtcaaacact tttcaaaatc ccaaacttgg tctggtaaat gagtaaggga tccgttgaag
    18061 ttaatcatag gagtgtctaa gcccagttca cggtaaaaat cttttgacat acggtaaggg
    18121 cgacctgtcg taataataac ctgatggcct ttttcagcaa cttttttgat ggtttttttg
    18181 gtaaagtcag aaatttgact gtctgagttg agcagggttc catccaggtc aactgcaata
    18241 atttttttcg tcatatggac ctttctagtg tgttttcaga taagatgtct tctattataa
    18301 cagaaagcag gcagaaaagc agattccaaa caaaaaatga aattggatta atttcttaaa
    18361 caaatcaaac aaagacattg aaaaagtgaa tgataaatga tataattcta ttattgttcg
    18421 taaaaattaa aaggagattg atgatggaca aattatttaa actaaaagag aacggtacag
    18481 acgttcgtac agaggttctc gctggtttaa caactttctt tgcaatgagc tatattctct
    18541 ttgtaaaccc acaaatactt tcacaaacag gaatgcctgc tcagggcgtc ttcctagcga
    18601 cgattattgg tgcagtagcg ggtaccttga tgatggcttt ttatgctaac ttaccttatg
    18661 cccaagcgcc aggtatggga ctcaatgcct tctttacctt tacagttgta ttcgggcttg
    18721 gttattcttg gcaagaagcc ctagctatgg tcttcatctg tgggattatt tcattgatta
    18781 ttaccttgac aaatgttcgt aaaatgatca ttgaatcgat tcccaatgct cttcgctcag
    18841 ctatttcagc tggtatcggt gtcttccttg cctatgtagg gattaagaat gctggacttt
    18901 tgaaattcac gattgatcca ggcaactata ctgttgtagg agaaggggct gacaaagctc
    18961 aagcaacgat tgcagcaaac tcttcagcag ttccaggatt ggtcagcttt aataatccag
    19021 ctgttttagt ggctcttgca ggacttgcca ttactatctt ctttgtcatc aaagggatta
    19081 aagggggaat tattctctct atcttgacaa caactgttct tgctattgca gttggtttgg
    19141 ttgatttgtc tagtatcgat tttgctaata accatgttgg tgcagctttt gaagatttga
    19201 agacaatctt tggtgcagct cttggttcag aaggtttggg agctttggtt tcagatacag
    19261 ctcgcttgcc tgaaactctg atggccattc ttgccttctc attgacagat atttttgaca
    19321 caattggtac cttgatcggt acaggtgaaa aagttggtat tgtagcgaca aatggtgaaa
    19381 atcaccaatc agccaaattg gataaggctc tttactctga tttgattgga acgacagtcg
    19441 gtgccattgc aggtacttca aacgtaacga cttatgttga gtctgctgct ggtatcggtg
    19501 caggtggacg tactggtttg acagccttgg ttgtagctat ctgttttgcg atttcaagct
    19561 tctttagccc acttctagcg atcgtaccaa cagcggctac agctccaatc ttgattatcg
    19621 ttgggattat gatgcttggt agcttgaaaa atatctattg ggatgatatg tctgaagcag
    19681 ttcctgcctt cttcacatct atctttatgg gattcagcta ctctatcact caagggattg
    19741 cagttggttt cttgacttac actttgacta agcttgtcaa aggtcaagtt aaagatgttc
    19801 atgccatgat ttggattttg gatgccttgt ttatccttaa ctacatcagc atggccttat
    19861 aatagaatga cccaggggga ttttccccct tttttaatac aaaggagata ggtgatgaaa
    19921 gagaaaaata tgtggaaaga attgttgaat cgtgcaggct ggattttggt ctttttactt
    19981 gccgtccttt tatatcaggt tcccctagtg gttacctcta ttttgacttt aaaagaagta
    20041 gccctgctac agtcagggct gatagttact ggtatttcta ttgtggttct ggtgctattt
    20101 attattggag ctcgtaaaac gcaattggca agttttaatt tttctttttt tagaactaag
    20161 gatttggcac gtttgggctt gagttattta gtcattatct gttcaaatat acttggttct
    20221 atcttgttgc aactgtcaaa tgagacgaca acagctaacc agtctcagat taacgatatg
    20281 gttcagaata gttctctgat ttctagcttc ttcttgctag ccttgcttgc tccaatttgt
    20341 gaggaaatct tgtgccgtgg gcttgttcct aaaaagattt tccgaggcaa ggagaacttg
    20401 ggatttgtag tcggtacgat tgtgtttgct ttattgcatc aaccaagtaa tttaccttct
    20461 ttattgattt atggaggtat gtcgacagtt ctatcttgga cggcctacaa gacccaacgt
    20521 ttggaaatgt cgatcttgct tcacatgatt gttaatggga ttgctttctg tttgttggct
    20581 cttgtggtga ttatgagtcg gacattagga atttctgttt aaaagttttt atgtaggaac
    20641 cgacctcttt ctactaggga aagatgaatg caatcgtgtc catctttttc tttttatggt
    20701 aaaatagaaa aataatatga tgaaaatcct tgagggagtg accgatatgt caagtaaagc
    20761 caatcatgca aagacagtta tttgcggaat tatcaatgta accccagact ccttttcgga
    20821 cggtggtcaa ttttttgctc ttgagcaggc actccagcag gctcgtaaat tgatagcaga
    20881 aggagccagt atgctcgata tcggcggaga atcgactcgg ccgggaagta gctatgttga
    20941 gatagaagag gaaatccagc gtgttgttcc agtgatcaaa gcgattcgca aggaaagtga
    21001 tgtcctcatc tctattgata cttggaagag tcaagtagca gaggctgctt tggctgctgg
    21061 tgccgatcta gtcaatgata tcactggtct catgggtgat gaaaaaatgg cccatgtggt
    21121 tgctaaagct ggagcgaaag tggtcatcat gtttaaccca gttatggctc gacctcagca
    21181 tcctagttcg cttatcttcc ctcattttgg ctttggtcaa acctttacag aaaaagagtt
    21241 agctgacttt gaaacattgc caatcgaaga cttgatggtg gctttctttg aacgagcact
    21301 agcgagagcg gcagaagctg gtattgcacc agaaaatatc ctgttggatc caggaattgg
    21361 ctttggtctg accaagaaag aaaatcttct ccttttacgg gacctggata aactacatca
    21421 gaagggctat ccaatctttc tcggagtgtc gcgcaagcga tttgtcatca atatccttga
    21481 agaaagtggg tttgaagtca atcctgagac agagcttggt ttccgaaatc gggacacggc
    21541 ttcggctcat gtaactagta tcgctgcgag acagggtgta gaagtggtgc gcgtgcatga
    21601 cgtagctagt cacaggatgg cagttgaaat tgcctctgcc attcgtctgg ctgatgaagc
    21661 ggaaaattta gatttaaaac aatataaata agatgaaaga aattgaaaac aatcagtgga
    21721 ttgctaacta ctggacggat caaccgcatt ttggcttgga acgaatggtg gaactgttag
    21781 ctttgcgtgg caatccccat cttaaactca aggttctcca tatcggaggg actaacggca
    21841 agggctcgac tattgctttt ttgaaaaaga tgctagaaaa gctagggctg agagttggcg
    21901 tgtttagttc gccctatctc attcattaca cagaccagat tagcatcaat ggggaatcga
    21961 tctcagaagc gaggctagaa gctctcatgg cagactatca gtctttgctg gagggagaag
    22021 cggtcgccaa tttacagggc acaaccgagt ttgagattat cacagcccta gcctatgact
    22081 actttgcctc agagcaagta gatgtggcta tcatggaagt tggcatgggt ggacttttgg
    22141 atagtaccaa tgtctgtcag cccattttga caggaattac aactattggc ttggatcatg
    22201 tggccctact tggtgacacc ttggaggcca tagcagagca gaaggcaggt attatcaaac
    22261 aagggatgcc cttggtaaca gggcgtattg ctccagaagc cttgactgtg attgaccgca
    22321 ttgcggaagg gaaagatgcg ccgagacttg cctacgggac agattatcag gttcgtcatc
    22381 aagaaagtgt ggtgacaggg gaagtctttg actatacaag tgctgtcaga caaggtcgct
    22441 tccagactag cctgcttggt ttgtaccaaa tagagaatgc tgggatggcc atagctttac
    22501 ttgatacttt ttgtcaagaa gatggtcgag agctagcaag caatgacttt cttggtcaag
    22561 ccttggaaga aacaagttgg ccagggcgtt tggaaatcgt gtcaagagat cccttgatga
    22621 ttttggatgg agcccataat ccccatgcta tcaaggcctt gttggtaacc ttgcaagaac
    22681 gttttgcgga ttatcataag gaaatcctct tcacttgtat caaaaccaaa gccttggggg
    22741 atatgttgga cttgctggga gctatgccag ataccgagct tactctaaca cattttgcgg
    22801 atagtcgggc gacggatgaa agcgtgctga aagaggcagc taagtctaga aatctcagct
    22861 accaagattg gcatgatttt ctagagcaga atttgacaga taaaaaagaa gagaaacaaa
    22921 cagttaggat tgtcacaggt tccttgtatt tcttgagcca agtgagggcc tatctgatgg
    22981 agaggaagaa cgagaatgga tacacaaaag attgaagcag ctgtaaaaat gattatcgag
    23041 gctgtaggag agaacgctaa tcgcgagggc ttgcaggaaa cacctgctcg tgtagcccgt
    23101 atgtatcaag agattttttc aggtcttggt caaacagcag aggaacattt gtcaaaatcc
    23161 tttgaaatta ttgacgataa tatggtggta gaaaaggata tctttttcca taccatgtgt
    23221 gaacaccact tcttgccatt ttatggtaga gcgcacattg cctacattcc agatggtcgt
    23281 gtggcaggct tgtctaagct agcccgtacg gttgaagttt attcgaaaaa accacaaatt
    23341 caagaacgtt tgaatatcga agtggccgat gccttgatgg actatctagg tgctaaagga
    23401 gcctttgttg tcattgaggc ggaacatatg tgtatgagta tgcgtggtgt tagaaaacca
    23461 ggcactgcaa ccttgacgac agtagctcgt ggtctatttg aaacagataa ggacctccgc
    23521 gaccaagctt atcgtttaat ggggctataa aaagaatccg tttcaagcgg atttttctag
    23581 aaaggaatca ttatggatca actgcagatt aaggatttgg aaatgtttgc ctatcatggt
    23641 ctttttccta gtgagaaaga attggggcag aaatttgtcg tttcagccat cctatcctat
    23701 gatatgacca aggcagctac agacttggat ttaacagcct ctgtccatta cggagaattg
    23761 tgtcagcagt ggacgacttg gtttcaggaa acgagtgaag atttgattga aacggtagcc
    23821 tataaactgg tggaacgtac ctttgagtct tatcctcttg tccaagaaat gaagttggaa
    23881 ctgaaaaaac cttgggcacc ggtgcatttg tcactagata cttgctcggt aaccattcat
    23941 cgccgcaagc aacgagcctt tatcgcccta ggaagcaata tgggagataa acaagcaaac
    24001 ttgaagcaag ccattgacaa actgcgagct cgtggcatct atattctcaa agagtccagt
    24061 gtcttagcga cggagccttg gggtggagtg gagcaggata gctttgccaa tcaagtggtt
    24121 gaggtggaaa cctggctacc agcacaagac ttgttagaaa ccttgttagc cattgagtca
    24181 gagctgggac gggtgagaga agtgcattgg ggacctcgtt tgattgattt ggacttgctc
    24241 tttgtggagg accagatcct ttatacagac gacctcatat tgcctcatcc ttacatagcg
    24301 gaacgccttt ttgtccttga gtccttacag gaaattgcgc ctcattttat ccatccgata
    24361 ttaaaacaac cgatccgtaa cttgtatgat gctttgaaaa aatagaaaaa ctctagtttt
    24421 cagttacttg caactgaagg ctagagtttt taaaataggt cattttcttc tgggaggagg
    24481 atagtttctc taccgtccat gtctaaaacc agtactcttg ggggataacg agggtcgaaa
    24541 ggatggttaa agtcaaaatc aatggctgta gggaggtgtt gacttgaaaa gtggaaggta
    24601 atctttcctt ggttattaag caattgaaac tcgagttctt cttccaattc aaagacattt
    24661 tttaagaaat ggtcgataat ataccaaaaa gagtcaatga tgtcatcagg caagctggta
    24721 acaatgccaa aactagcaga ccgcatgtgg gtattggtaa aagccatatc tctgtctcct
    24781 ttcttttccc ttatcataca gcaaatagga ttaaaaatca agaaaaggtg attttttgaa
    24841 aaggatttta actacaggga aaaagaagga aaaaaattcc caaaaatcta ccgaagttaa
    24901 taggtaaatt cccaaattaa cttgattata taactttcag ttactttgag aagttaccga
    24961 aaaatatttt tcatatctat tgacttttag gggtgaaatt tggtatgata gtaggcggta
    25021 ttgtttaccc catttgaaag gccccggaac cttccaaata cttttcgatg ggaaggaaca
    25081 cccatcaccg taaacaaaaa tcgaactata tataggagaa atcatgaaca aaacaacatt
    25141 tatggctaaa ccaggccaag ttgaacgtaa atggtacgta gttgacgcaa ctgatgtacc
    25201 acttggacgt ctttctgcag tagttgctag cgtacttcgc ggaaaaaaca aaccaacatt
    25261 tacaccacac actgatacag gtgacttcgt gattgttatc aatgctgaaa aagttaaatt
    25321 gactggtaaa aaagcaactg ataaaatcta ctacactcac tcaaaccacc caggtggatt
    25381 gaaacaaatc tctgcaggtg aacttcgttc taaaaatgca gtacgtttga ttgagaaatc
    25441 agttaaaggt atgcttccac acaatacact tggacgcgct caaggtatga agttgaaagt
    25501 atttgttgga gctgagcaca ctcatgctgc acaacaacca gaagttcttg acatttcagg
    25561 acttatctaa ggaaaggaac aaataaagta tgtcacaagc acaatatgca ggtactggac
    25621 gtcgtaaaaa cgctgttgca cgcgttcgcc ttgttccagg aactggtaaa atcactgtta
    25681 acaaaaaaga tgttgaagag tacatcccac acgctgacct tcgtcttgtc atcaaccaac
    25741 cattcgcagt tacttcaact gtaggttcat acgacgtttt cgttaacgtt gtaggtggtg
    25801 gatacgctgg tcaatcagga gctatccgtc acggtatcgc tcgtgccctt cttcaagtag
    25861 acccagactt ccgcgattca ttgaaacgcg caggacttct tacacgtgac tcacgtaaag
    25921 ttgaacgtaa gaaaccaggt cttaagaaag ctcgtaaagc atcacaattt agtaaacgtt
    25981 aattcgaaag aattactata cttatacaga gcacctttcg gggtgttctt tttttatact
    26041 ttcttactaa attggtgcaa ttgacacagt tgttgcgact ttagtcgctt acaaatgtgg
    26101 ctgcaacctg acaaggtcag ttgcctcaaa acgttaatca atacaattat atcaacgttt
    26161 caaagcactc aagagtttac ctcatgggtg ctttttttat gttttttgaa aaagttcacc
    26221 tcaaagttta ccttattttc accttattgt tttagagact gtaaggcaaa ttcaccgacg
    26281 gccataggga ctacattaga agtattgaca taaatctgtg ttgtacctgt gtcgctatgt
    26341 attagagctt cagaaatggc ttctaagctc attcctgctt gtttagcgag cgttgctccg
    26401 gtatgacgta gtttgtgtgg cgtagcgtgt gtaagctctg ggtgtcgttt tctgatagtt
    26461 ttcatcttat tattgagata gtcagaatgt aaaggtttat tgatattgcc tttggtatct
    26521 atgtaagtaa agatgaattg ttctggatta ctgatgatac caaactttgc cagttcatat
    26581 ttttgttgtt ttttccatga agtcagaagt tgaagtaagt cattagaaat agagaaaatg
    26641 ggaaatgttt ccacaataaa acgcatatta tcagatggtt aatgcgtttt atatttgggt
    26701 ttgaggctta taaaatagga taagcgtact gaaaacttag atatgacaaa caaaataaaa
    26761 tttttaaaag ctacagagaa acatggtata atgtatctaa aggaggtagc atatgtcctt
    26821 agatatagat aaagaaaaga tgacaatcat gggaatagct tttgaaaacc gatccgtttt
    26881 taagagtgtt tggtatgctt taagtacaaa tatgattgaa ggatggcgac ctacggttag
    26941 tgatgttgaa aaattacgag atgaagctct tgctctgggg atgacttaat gaagcgcaac
    27001 tgttatgagt cttatgacta tgttgatccg gataatttat ataccgatcc gggttcttca
    27061 gttctaagaa ataagcagga aggaaaaaag cacgcgagct tgaatatcgt atgattgcta
    27121 gtaagagttt gaaattattt atcaatccta tcttagttaa tagtttaaaa gtagtttgtt
    27181 tcacaactgc ataagcttag aaaagttgaa aagtgataac tctttaataa actcttggtt
    27241 tgttaaaaga tattttgtcc actacatttt ttcaattgac tattatttgt catagatata
    27301 acagatagaa cattgcattc agaataatga aaaacgcatc aaaaatggtg cgttctgttt
    27361 ttatgttacc ataaaaggag gaaaacaaat tgaaaatttt taagggagag ttttatcgaa
    27421 tctctgtatt aacagacaag ctagtaaggt tagaatactc tcaaactgga agttttgagg
    27481 atagaacgac acaacttatc tataatagag attttggcca agtttcgtta gattatatcg
    27541 agacatcaaa cgtactagat attatgacgg actattttca tctgcacttt aataaaggag
    27601 aatttaacgc cgaaaattta tttatagaat taaaaggaaa ttttgccgta tatggtagtc
    27661 gctggtattt tggtgaatct attgaaacgt taaaaggaac agctcggact ctggataagg
    27721 cagatggagc aatctcgtta gaagatggaa ttattagccg aaatggtata gccttattgg
    27781 atgattctca aggatttatt tgggatgaac aatctggtta tattgagaga gaaaatcaaa
    27841 ttgacctgta tttctttgcc tatgggcatg attatagagg agcaatcaga gacttttacc
    27901 atttgactgg ttcaacaccc ttgttgccaa gatatgcttt aggcaattgg tggagtagat
    27961 attggcctta tacgtcggat gaatacttgg atttaataga cagatttgca acagagaaaa
    28021 ttccattatc tatcggtgtg ttagatatgg attggcatat aactgacatt ccagctcgtt
    28081 ttggaagtgg ctggacagga tatagttgga atagaaactt aataccaaat ccagaacagt
    28141 tattgcaaca acttcatgat agaaagttaa aactctcctt aaatgtccat cctgctgatg
    28201 ggatacgggc ttatgaagaa gcttatcctc aaatcgcaaa acggttgggg ttaaatgtag
    28261 aactagaaga acctgctatt tttgattttt ttaatccctc ttttagggaa gcctacttta
    28321 aagatgttca ttatgagcta gaaaagcagg gagtagattt ttggtggatt gactggcaac
    28381 aggggacaca aggcatgcta gatccacttt ggcttttaaa ccattatcac tatcaggata
    28441 gttgtaaaaa tgcagaaggt ggtttgattt tatcaagata tgcaggtcct ggtagtcacc
    28501 gctaccctgt tggtttttca ggggatacta ttatcagttg gaattcctta agatttcaac
    28561 cctattttac agcgacagca tctaatatcg gttatagttg gtggagtcat gatatcggtg
    28621 gacatatatt gggggattat gacgaagagc tacaaactag atggctacag tttggtgttt
    28681 ttagtccgat tactcgatta catagttcta gaagtccttt taatagtaaa gaaccttggt
    28741 ttttttcaga aacaacatct aagattatga agaaatacct tcgtttgaga catcagatga
    28801 ttccttatct atataccatg aatgtaaaga cacatgagga aggtgcccca ttaatcagtc
    28861 caatctatta tttctatcca gagaatgatg agagctataa tgttccaaac caatactttt
    28921 ttggaacaga actgatggtg gctcccattg tagaaaagat ggatttgaca ttccaatctg
    28981 caaaagtaga tgtatggttc cctgaaggtg aatggtatga cttcttttca gagaaaaaat
    29041 acacaggtgg tgtgaagtta agtgtttata gggacatctc gactacgcct gtgtttgcaa
    29101 aaagtggtgc aatcattccc ttggttggtt ctgagatagg tatgggtgtt gatttacctg
    29161 aggttgtaga ttggtatgta ttcccaggga aacaacattc ttttgaaatg cttgaagatc
    29221 aaaatggtca aagatataaa acaagattat caatcgattg ggaaatggga atggtagagt
    29281 tagcattaca aggagattct agtatcgttc caagcaatag aaaacataga attcatttta
    29341 aaggaacgaa tgtgtctata attgaattgc caaataagaa tgatacagct aaatttgaat
    29401 ggaaagataa taaaaggaca tctttaaatg acgaagtttt tagactacta aagacagctt
    29461 ctcttccata tgaattaaaa gatagattgt taaatcaatt catcaatgcg aaaaactctc
    29521 atgacttaat gaatatcttg catcatcagg ataaggaatt gagagggcgt ttgttggaaa
    29581 tgatatttac tagccaaaac taattgaaat aattagaaag tcatttgttt agtgtatatg
    29641 aagattctgt atggtattcg ttatcactgg tcacattgaa aatgtgaaat aagcacctac
    29701 aacatagact ttacaacact tcatgtttca tatcatgggg tgtttttcta tttaagtcaa
    29761 agccaatcaa aaaatctcac tattcatcag gttaaaggaa acctaatcat agtgagattg
    29821 tgaattataa tagagtttgt atcgcctctt caatttgttt tgggtctgtt tttgaagaaa
    29881 agcgttcaaa gacttgccca tcacgaccaa tgagaaactt agcgaaattc cattcgattc
    29941 gttttcctag tgggccagat ttatggtctt ttaaccagac atagagaggg tctgcttcct
    30001 taccgttgac cttaatcttg gcaaaacgtg ggaaggtggt ttgaaaatgt aggctacaga
    30061 aggtgttgat ttcctctgcg ctgccgggtg cttgtcccat aaactgattg caagggaaat
    30121 ccaatatttc gaagccctga tcttgatagc gttcatagag ttcttgaagt ccctggtact
    30181 ggggcgttaa accacatcca gtagcagtgt tgacaatcaa gagaactttg ccacgatagc
    30241 tatccaaggg agttgcttgg ttgttttggt tcaaaacgga aaaatcataa agtgaagtca
    30301 tgagggcctc ttttcttttt ctacattgta acagttttta cttttttcgt cgagtcggat
    30361 ggaagatatt gttgaagaat aagccctcaa tatgatagaa accgtttaca aataacttta
    30421 aaaatggtat gatttaatta aaccggttta ctttgttgct ataaatctct aatttgagat
    30481 ttagttccta ttgattttac aatatgttta ttggagtgta tacatgcaaa caaaaacaaa
    30541 gaagctcatt gtgagtttgt cttcacttgt tttatcagga tttttattaa accattatat
    30601 gacagttgga gcggaagaaa cgactacgaa taccattcag caaagccaga aggaagttca
    30661 gtatcagcaa agggatacaa aaaatttagt tgaaaatggt gattttggtc agacggagga
    30721 cggaagcagt ccgtggacag gaagcaaagc tcaggggtgg tcagcttggg tagaccagaa
    30781 gaatagttct gcagatgcct caactcgagt cattgaggct aaggatgggg ctatcactat
    30841 ctcaagccct gagaaattaa gggcagcggt tcaccgtatg gttcctattg aagctaagaa
    30901 aaagtataaa ctgcgtttca agattaaaac agataataaa gtcgggattg ccaaagttcg
    30961 tatcattgag ggaaagtggt aaggacaagc gattgtggaa ttctgcaacg acgtcaggaa
    31021 caaaggactg gcagaccatt gaagcagact atagcccgac tttagatgtt gataaaatca
    31081 agctggagtt attctatgaa acaggaactg ggactgtttc ctttaaggat attgagctgg
    31141 tagaggtagc agaccagcct tctgaggatt ctcaaacaga taaacagctt gaggaaaaga
    31201 ttgatttacc aattggaaaa aaacatgttt tttctcttgc ggactatact tataaggtag
    31261 aaaatcctga cgttgcttca gtcaaaaatg gaattttaga acctcttaag gaagggacaa
    31321 ccaatgtcat tgtcagtaaa gatggcaagg aagtgaaaaa gattcctttg aagattctag
    31381 cctctgttaa ggatgcatac acagaccgtt tggatgactg gaatggcatc atcgctggga
    31441 atcaatacta tgattctaaa aatgaacaga tggccaaatt aaaccaggaa ttggaaggaa
    31501 aggtagctga tagcctatcc agtatttcaa gtcaggcgga ccgcatctat ttgtgggaaa
    31561 aattttcaaa ttataaaacg tctgcaaatc tgactgccac ttatcggaaa ttggaggaga
    31621 tggccaagca agtgaccaat ccttcttctc gttattataa agatgaaact gtcgttcgaa
    31681 cagtcaggga ttccatggaa tggatgcata aacatgtcta caatagtgaa aagagcattg
    31741 ttgggaactg gtgggattat gaaattggta cacctcgtgc catcaacaat accttgtctc
    31801 tgatgaaaga atacttctct gatgaggaaa ttaaaaaata tacagatgta attgaaaaat
    31861 ttgtaccaga tcccgaacat ttccgaaaga cgactgataa cccatttaag gctctaggtg
    31921 gaaacttagt tgatatggga agagtaaaag taatagctgg tttactgcgt aaagatgatc
    31981 aagaaatttc ttctaccatt cgctcgattg agcaagtgtt caagttggta gaccaaggtg
    32041 aaggttttta tcaagatgga tcctatatcg accataccaa tgttgcctat acgggtgctt
    32101 atgggaatgt tttgattgat ggcctgtctc aactgttgcc agtcattcaa aagaccaaga
    32161 atccaatcga taaagataaa atgcaaacca tgtaccactg gattgataaa tcgtttgctc
    32221 ctttgctggt gaatggagag ctgatggata tgagtcgtgg acgctcgatc agtcgtgcaa
    32281 atagcgaggg gcacgtggcc gcagtagaag tactaagagg gattcaccga atagcggata
    32341 tgtctgaagg agaaaccaaa caacgtttgc agagtcttgt gaagaccatt gttcaatcgg
    32401 atagttatta tgatgtcttt aagaatttga agacttataa ggatatcagt ttgatgcaat
    32461 ccttgttaag tgatgcagga gtcgcaagtg ttccaagaac aagttaccta tctgccttta
    32521 acaagatgga taaaacagcc atgtacaatg cagagaaagg gtttggattt ggcttgtcac
    32581 tcttttccag tcgtaccttg aattacgaac atatgaacaa ggaaaataaa cgtggttggt
    32641 atacgagtga tgggatgttc tatctttaca atggcgattt gagtcactat agcgatggct
    32701 actggccaac agtcaatcca tataagatgc ctggtacaac agagacggat gctaagagag
    32761 cggatagcga tacaggtaaa gttttaccgt ctgctttcgt tggaacgagc aaactagatg
    32821 atgccaatgc gacagcaacc atggatttca ccaactggaa tcaaacattg actgctcata
    32881 agagctggtt tatgctaaag gataagatcg cctttttagg aagcaatatc caaaacactt
    32941 caacagatac tgctgcaact acaattgacc agagaaaact ggaatcaggt aatccatata
    33001 aagtctatgt caatgataaa gaagcctccc ttacagaaca agaaaaggat tatcctgaaa
    33061 cccaaagtgt ctttttagaa tcgtccgatt cgaaaaagaa tattggttac tttttcttta
    33121 agaagagttc aatcagtatg agtaaggctt tgcaaaaggg agcctggaag gatatcaatg
    33181 aaggacagtc agacaaggaa gttgaaaatg aatttcttac gattagtcag gctcataagc
    33241 aaaatgggga ttcttatggc tatatgctca tccctaacgt ggatcgtgcc accttcaatc
    33301 aaatgataaa agagttagaa agtagcctca tcgacaataa cgaaaccctt cagtctgttt
    33361 atgatgccaa acaaggagtt tggggcattg tgaaatatga tgattctgtc tctactattt
    33421 ccaaccaatt ccaagttttg aaacgtggag tctatatcat tcgaaaagaa ggggatgaat
    33481 ataagattgc ctactataat cctgaaaccc aggaatcagc tccagatcag gaagtcttta
    33541 aaaagctaga gcaagcaact cagccacaag tacagaattc aaaagaaaag gaaaaatctg
    33601 aagaggaaaa gaaccattcg gatcaaaaga atctccctca gacaggagaa ggtcagtcaa
    33661 tcttggcaag tctagggttc ttgctacttg gggcgtttta tctattccgt agaggaaaga
    33721 acaactaatt tgttcatcat ctagatgaat tacatgaaac tgtggaaaat agaaaaatcc
    33781 gaagaaggcc aaatatcttc ggatttttct tttaaaaaag ataagttgat acttatcctt
    33841 ttttacttca ttatgtgact atagcgtttt gaaactagaa taggacgtta taactgctaa
    33901 aagatttcta taaattcatt tgattttcct aatcaatttg ttcgtatcct atttcactcc
    33961 actatattag tccgtctcgc tccgtgaggt gcgcgtcgaa ggttatacca aaaaaactcc
    34021 aaacgcgata caataaaggt gttcaatcca attgtaaagc gaaaggagaa aaaatatggc
    34081 acaaaaggct catagtttat ctcacacaaa gtggaggtgt tctatcacat tgtgttcacc
    34141 cctaagtata gacgaaaagt catctataat caatatagaa gtagtttagg cgaaatattt
    34201 cattgcttgt gtagttataa aggtgttgaa atgatcgagg gtcacttaat gccagaccat
    34261 gtacacatgt tagtcagtat tccgtcgagg ctaagtgttt caagttttat ggggtattta
    34321 aaaggcaaaa gtgcactcat gatgtttgac aaacacgcca atctcaagta caagtttggg
    34381 aatcggcatt tctgggtgga aggttattat gtaagtacag tagggcttaa taaagctaca
    34441 attaagaaat atagtcaaga ttaaagaaat tatccagtgg atgatttctt cacgagtatg
    34501 aaaatttgag aactagtaaa gtatgatata tagtaaaatg aaataagaac aggataaaat
    34561 caatcaggac agtcaaatcg atttttaaca atgttttaga agtagaggtg tactattcta
    34621 gtttcaatct actatagcac tagataaatt gagtgtaaaa gaatatgagg atccctttag
    34681 ggatagtggt aagtaatacc aaagtctctt aaagaggcaa gtgacgagtc aagagcaata
    34741 aggcttgaac aacgtgaaag ccagcgtctt taggcgctgg ctgatgattt gggcttatag
    34801 ctctgagata aaccacccgt tagacgggta gttatgattt tatctgagtg taatatactg
    34861 ttgggcaatc tcgctgatgc ggtcaaagtt gccttgggaa gcgagtttat tgagttcgcc
    34921 accaattcca acggcatctg caccagcagc gaaccattga gggatgttgt ttaggccgac
    34981 tcctccggtt accattacgg aaacttgtgg gatcggtgcc ttgactgcag agatatatgc
    35041 tggactgaga gtactacctg ggaagagttt gatgatttca ctaccggctt caagtgcagt
    35101 cgtgatctct gtgagggtaa tacagcctgg aatgtacggt gtgctgtaga gattgcacat
    35161 tttcgcagtt tcagcatgga aagatggaga aacaacgtaa tttgctccag ctagaatggc
    35221 atctctagca gttacggcat caagcacagt acctgcaccg atacaaacac tctgatcgtc
    35281 ctgatacaag tctacaagtt ccttgatgat ttgtcctgca tactgattgg tataggcgat
    35341 ttcaatagct ttgataccgc ccttgataca agcaatcgag gcttgtagtc cttcttcctt
    35401 tgtatttcct cgaatgacag cgacaatttt ctgttttttt agttcaataa tcgtatctga
    35461 tttggtcatg taattctcct aacgaatgat atcttgtgca tttgccagta aattttcaat
    35521 actagttgcg gaagtggaga gatggtctcc tggaagagtg catttgagag ttgcgctcgc
    35581 aactgcaaag tcaatggtag tttttaggga ggaatgatgg agtagttggt aaagggcacc
    35641 agatataaag gcatccccgc taccaattcg ttgatagact gcagttttta gttggacaga
    35701 ctcttcaaat cgttcttcca gagcataggc ttgatagaca tttttgtctt gctcatcact
    35761 agagcggagg gtatggaaaa tggccttgaa accataggct tcttttaaaa gtcgcatgcg
    35821 attttccacc tcttctaggc tagcactgtc tcttggaaac atctctagat tttggtcatc
    35881 aatcatgaga ggatcaatac cgaagcaata gtcagtaaaa cgtgcaaact tagaaaattc
    35941 atacttggct tctaggactg aaatcatctt tgttctcaga ttgagatcca ttgaaacgac
    36001 aattcctcgg cgcttggctt cttccaagag taggagaagg atcgcacgga cctcttgacc
    36061 gatagctacg gtgattccac taaaatgaaa atggctaatc ccctgaaaga gagaatccat
    36121 atctagcatg tttggccgaa tctggctgat actcgtatgc ttacgatcgt agaaaacttc
    36181 actttgacga caaccaaagc cgttctccaa atagtagagg ccgattcgat cgccaagccg
    36241 acagattgaa ctggtatcga tttggtgctg tttcaagaat gtgagaaaac gatctccaat
    36301 ctcgttggca gggagtgcgg taaagacttt cgttgagata cccagggctt gcaaattaca
    36361 agcgatgtta atttctgatc cgccaaaata agtcgaactg gcaacatgat cgccgatact
    36421 ggtggcatct aatggtgaga ttcgaattaa tggttcgcca aacagtaata ttttcgtcat
    36481 ccattagttc ctttctggtt tagaatagct ttacttggct aatacttctt tcaaataagc
    36541 agcgacttca tcatcttggc agttttcaaa gaagtattct tggaaatatt tgccagagat
    36601 ggtgtctttg aggaagtctt ggtcgattgt tttaaggatg gtcatcaaat cattgtgggt
    36661 gatttgtttc acctcgttta agatacgagc gttgcgttgt tcagggatta cacgttctct
    36721 tgggtagcca ccgcccattt cttcagcaaa taagcgttca aacatcaatt tgagggtcag
    36781 ttctgccccc cagccaaatc ctttggcata agggatagac aaggcgttac caccattgat
    36841 ttgagaataa aggtaagcgt cagttgggtc cactgctaga ccacagacaa caccagggaa
    36901 gctgtttaaa gcaagcatag cccctacacc cgtaccacag ccggtaacaa caaagtcaac
    36961 tgcctttgta tttaaaagga tggcagccat tagtccgttc tgcacataag ttaattgact
    37021 ttctccttct tctccacgca taccatagtt aaataattgg tagccttttt tatctgtcgc
    37081 ttcttttaga ctatcgtaaa taatgtgatt cttgctagct tgactatttt cattgattaa
    37141 tgcgattttc ataagatctt ctttctattt ttactcaggt tgttttccga tgtaggctaa
    37201 gataccgcca tctacatata ggatgtggcc attgacaaaa ttgctggcat cactagcgag
    37261 aaagacagca gggcccatca aatcttcagg atttccccaa cgtgcagcag gtgtttttgc
    37321 aatgatgaac tggtcaaatg ggtggcgaga accatcttct tgcaactcac gaagaggtgc
    37381 tgtttgagga gtggcaatat aacccggtcc aattccgtta cattggatat tggctccacc
    37441 gtattcagac gcaatgttgc gggtcaacat tttcaagccc cctttagcag cagcataagc
    37501 gctaactgtt tcacgtccca gttcgctcat catcgaacaa atattgataa tctttccatg
    37561 ccctttcttt atcatagaag gaataactgc ctttgaaacg ataaatggtg cgtttaagtc
    37621 aatatcgatg accttacgga aatcagcggc gctcatttcg cacattggaa ctcggcggat
    37681 aataccagcg ttattaacga ggatgtcaat gacaccaacc tcttgttcga tttgcttgac
    37741 catggcttgg ataccgtcct cgtctgtcac gtcacagaca tatccatggg cttcgatgcc
    37801 aacttcacga taagcttcaa ttcctttatt gaccagatct tgattgatat cgttaaagac
    37861 aatagtagcg ccggcttcag cgtaggattt ggcaatagca aatccaattc cataagaagc
    37921 gccggtgatg agagcaattt ttccttttaa agaaaactgc tcaattgaga atgatgtatt
    37981 tgtcatttga tacttccttt caaatttgtt aagacttctc tataaccaaa t
//
LOCUS       DAVGJL010000006        33541 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.006, whole genome shotgun sequence.
ACCESSION   DAVGJL010000006 DAVGJL010000000
VERSION     DAVGJL010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33541
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.006"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(<1..498)
                     /locus_tag="VVE42_000245"
     CDS             complement(<1..498)
                     /locus_tag="VVE42_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEU3072515.1"
                     /translation="MRTYENKEELKAEIEKTFEKYILEFDNIPENLKDKRADEVDRTP
                     AENLAYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWFTDTYAHLSLQEL
                     KAKLNENINSISAMIDSLSEEELFEPHMRKWADEATKTATWEVYKFIHVNTVAPFGTF
                     RTKIRK"
     gene            complement(725..1594)
                     /gene="rsmI"
                     /locus_tag="VVE42_000246"
     CDS             complement(725..1594)
                     /gene="rsmI"
                     /locus_tag="VVE42_000246"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEU3072516.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQTLKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGASAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFGS
                     KKDYPETQIFYESPHRVADTLENMLEVYGDRSVILVRELTKIYEEYQRGTISELLESI
                     SETSLKGECLLIVEGASQDVEEKDEEDLFLEIQARIQQGMKKNQAIKEVAKIYQWNKS
                     QLYAAYHDWEEKQ"
     gene            complement(1597..1914)
                     /gene="yabA"
                     /locus_tag="VVE42_000247"
     CDS             complement(1597..1914)
                     /gene="yabA"
                     /locus_tag="VVE42_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEU3072517.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            complement(1954..2844)
                     /locus_tag="VVE42_000248"
     CDS             complement(1954..2844)
                     /locus_tag="VVE42_000248"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEU3072518.1"
                     /translation="MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFGSLEIAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQEEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLANQASFWTLVDESERLLT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            complement(2841..3479)
                     /locus_tag="VVE42_000249"
     CDS             complement(2841..3479)
                     /locus_tag="VVE42_000249"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEU3072519.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILEEKGVEVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLDLHKKVRQGYLSLLDKEGNRIVKIDASLPLEQVVETTKAVLFDGMGLAK"
     gene            complement(3699..4496)
                     /gene="proC"
                     /locus_tag="VVE42_000250"
     CDS             complement(3699..4496)
                     /gene="proC"
                     /locus_tag="VVE42_000250"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEU3072520.1"
                     /translation="MKIGFIGLGNMGASLAKSVLQTRTSDEILLANRSQAKVDAFIAD
                     FGGQASSNEEMFAEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLTLEKL
                     ASLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSELFCQLLSKAGLLVELGES
                     LINAATGLAGCGPAFVYLFIEALADAGVQTGLSRETALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEAHAFRGTVMEAVHQAYKRTQELGK"
     gene            complement(4500..5762)
                     /locus_tag="VVE42_000251"
     CDS             complement(4500..5762)
                     /locus_tag="VVE42_000251"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEU3072521.1"
                     /translation="MVSRQEQFEQVQAVKKSINTASEEVKNQALLAMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAMGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADEDKALSIINNAKTSRPSVCNAMEVLLVHENKAASILPRLDQ
                     MLVAERKEAGLEPIQFRLDSKASQFVSGQAAETQDFDTEFLDYVLAVKVVSSLEEAVA
                     HIESHSTHHSDAIVTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVAGDGQIRE"
     gene            complement(5772..6902)
                     /locus_tag="VVE42_000252"
     CDS             complement(5772..6902)
                     /locus_tag="VVE42_000252"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004240022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEU3072522.1"
                     /translation="MNNRSETMKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAML
                     HEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQI
                     VSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDELKVGDNDTLSA
                     QVAAMVQADLLVFLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTG
                     GMLTKIKAATIATESGVPVYICSSLKSDSMIEAAEETEDGSYFVAQEKGLRTQKQWLA
                     FYAQSQGSIWVDKGAAEALSQHGKSLLLSGIVEAEGTFSYGDIVTVFDKESGKSLGKG
                     RVQFGASALEDMLRSQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            complement(6982..8865)
                     /gene="cbpE"
                     /locus_tag="VVE42_000253"
                     /gene_synonym="pce"
     CDS             complement(6982..8865)
                     /gene="cbpE"
                     /locus_tag="VVE42_000253"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HEU3072523.1"
                     /translation="MKKKLTSLALVGAFLGLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWREGIETSYKHVLTDRVFRRLKELGVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDSRITNSERLWDNLYGYDKVLQT
                     AAEKGVSVIQNITQGDAHFQFGDMDIQLYNYENETDSSGELKKIWDDNSNSLISVVKV
                     NGKKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHHDTNKSNTKDFIKNLSPSLIVQ
                     TSDSLPWKNGVDSEYVNWLKERGIERINAASKDYDATVFDIRQDGLVNISTSYKPIPS
                     FQAGWHKSSYGNWWYQAPDSTGEYAVGWNEIKGEWYYFNQTGILLQNQWKKWNNRWFY
                     LTDSGVAAKNWKKIAGIWYYFNKENQMEIGWIQDKEQWYYLDVDGSMKTGWLQYKGQW
                     YYFAPSGEMKTGWVKDKEAWYYMDSTGIMKTGEIEVAGHHYYLEESGAMKQGWLKKAN
                     DWYFYKTDGSRAIGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVPIEKS
                     ATIKTTSHSEIKESKEVVKKDLENKETSQHESVTNSSTSQDLTSSTSQSSETSVNKSE
                     SEQ"
     gene            complement(8868..9755)
                     /locus_tag="VVE42_000254"
     CDS             complement(8868..9755)
                     /locus_tag="VVE42_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU3072524.1"
                     /translation="MEIKIETGGLRLDKALSDLSELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLPLEIVYQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLVLAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHSVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            complement(9745..10206)
                     /locus_tag="VVE42_000255"
     CDS             complement(9745..10206)
                     /locus_tag="VVE42_000255"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEU3072525.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMVLGLTLIIAGGLG
                     NFIDRASQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAMLKEEINGN"
     gene            complement(10203..11111)
                     /locus_tag="VVE42_000256"
     CDS             complement(10203..11111)
                     /locus_tag="VVE42_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU3072526.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLCEGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            11104..11301
                     /locus_tag="VVE42_000257"
     CDS             11104..11301
                     /locus_tag="VVE42_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU3072527.1"
                     /translation="MFIFYPQLLYLFIIPYFRSFPKRKKEHPILLNYLHYLPFSRQSY
                     FQNQFKKISGYTPLQYRKEKR"
     gene            complement(11353..12024)
                     /locus_tag="VVE42_000258"
     CDS             complement(11353..12024)
                     /locus_tag="VVE42_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU3072528.1"
                     /translation="MKKRAIQILLALSLIFYKSTWFWRVFNHLAKPYLPASREFFQIL
                     LLMESGVLFLAVIYLLVFAGKKIFHFKWQLRYFIYLLLGYIISYMSDFLFSYFISLSS
                     NQISLNETVEMMGRQEFPYFLLIVCFIAPIAEELIYRGVLMTTFFKNSPWYGDVLLSA
                     IIFGYIHINFALTPLAFFIYASGGLILALLYRMTKNLYYPILVHILINITAFWDVWLL
                     LFSGS"
     gene            complement(12078..12299)
                     /locus_tag="VVE42_000259"
     CDS             complement(12078..12299)
                     /locus_tag="VVE42_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0924 family bacteriocin-like peptide"
                     /protein_id="HEU3072529.1"
                     /translation="MILMTKNINLTNEELELIQGGADPYGKNPNGRYDWEIEPVLTLP
                     VHGFCPRGTYDLGYIGGGNHLCKGSAARF"
     gene            12571..13380
                     /locus_tag="VVE42_000260"
     CDS             12571..13380
                     /locus_tag="VVE42_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU3072530.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHITAIHKAIEKGVKLVLCTGR
                     PLFGVLPYYKKLELDLQNEYIIVNNGCSTHQTSDWSLVDWKELSPADIEYLYDLAEKS
                     DVQLTLFDESHYFVLGGKPNQVIENDAKLVFSDLTEISLEEATSGKFRMFQGMFLGTK
                     EQTDDFEQHFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYVKSLADAVTSSNEEDGVARAIEKYIL"
     gene            complement(13561..14436)
                     /gene="aguB"
                     /locus_tag="VVE42_000261"
     CDS             complement(13561..14436)
                     /gene="aguB"
                     /locus_tag="VVE42_000261"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HEU3072531.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKVIAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGEVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYDLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(14446..15531)
                     /gene="aguA"
                     /locus_tag="VVE42_000262"
     CDS             complement(14446..15531)
                     /gene="aguA"
                     /locus_tag="VVE42_000262"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HEU3072532.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKAAKRAF
                     TQIIETIAEGERVYLLVEQAYLSEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEALERPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVA
                     AFVGPAEVVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPAVRQVVTE
                     EDLPGYIYEEGEEERYAGERLAASYVNFYIANKAVLVPQFEDVNDQVALDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            complement(15528..16655)
                     /gene="nspC"
                     /locus_tag="VVE42_000263"
     CDS             complement(15528..16655)
                     /gene="nspC"
                     /locus_tag="VVE42_000263"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HEU3072533.1"
                     /translation="MKLEQVPTPAYVIDLAKLEANCCILQYVQEEAGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDADLEELLEITDHIVF
                     NSERQLRKHGPRCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGFHFHTLCEQGADDLQTTLKAVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPPLRNGFEAQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            complement(16655..17914)
                     /locus_tag="VVE42_000264"
     CDS             complement(16655..17914)
                     /locus_tag="VVE42_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HEU3072534.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTKIETAALDADKVEEVIALIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV
                     KDGVKKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLSWVVVENPQMVD"
     gene            complement(17911..18771)
                     /gene="speE"
                     /locus_tag="VVE42_000265"
     CDS             complement(17911..18771)
                     /gene="speE"
                     /locus_tag="VVE42_000265"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEU3072535.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQAFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDKEGWKKRQLFTEYYTANLHVGSFMLPKYVE
                     DILEEEEGKK"
     gene            19152..19741
                     /locus_tag="VVE42_000266"
                     /pseudo
     CDS             19152..19741
                     /locus_tag="VVE42_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809094.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(19707..21182)
                     /locus_tag="VVE42_000267"
     CDS             complement(19707..21182)
                     /locus_tag="VVE42_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU3072536.1"
                     /translation="MKELDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTDKLPISAMDAG
                     ADMAAVSMHKSGGSLTQSSILLIGEQMNSEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEKVIELSEYARREINAIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSERKSLTLDDSVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVIKRKTNYKKSQ"
     gene            21889..22938
                     /locus_tag="VVE42_000268"
     CDS             21889..22938
                     /locus_tag="VVE42_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEU3072537.1"
                     /translation="MQDNYTTKAKHLTIDSRRLIERWKKEGKSNREIASLLGKAPQTI
                     HTEIKRGTVRQCLGKGRFKEVYSADYAQQSYENNRKRSVKKSSLTKELKEKILHYHNQ
                     KFSPEMMVIAKGVNVGISTIYYWIHRGKLGLSKQDLLYPRKGKALKKQASTNFKPAGQ
                     SIEQRPEAINLRLENGHYEIDTVLLTRAKNYCLLVLTDRKSRHQIIRLIPNKSAEVVN
                     QALKLILKQHKILSITADNGTEFNRLFDVFSEEHIYYAHPYASWERGTNENHNRLIHR
                     WLPKGTKKMTPKEVAFIEKWINNYPKKCLNYKSPREDFWMANLNLKFSVRNKSRNRFK
                     FCRSFKYPARRCSWC"
     gene            22910..23554
                     /locus_tag="VVE42_000269"
     CDS             22910..23554
                     /locus_tag="VVE42_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEU3072538.1"
                     /translation="MLRAGVLGANDGIISIAGVVIGVASATTNIWIIFLSGFAAILAG
                     AFSMAGGEYVSVSTPKDTEEAAVSREKLLLDQDRELAKKSLYAAYIQNGECKTSAQLL
                     TNKIFLKNPLKALVEEKYGIEYEEFTNPWHAAISSFVAFFLRSLPPMLSVTIFPSEYR
                     IPATVLIVGVALLLTGYTSARLGKDPTRTAMIRNLAIGLLTMGVTFLLEQLFSI"
     gene            complement(23596..25584)
                     /locus_tag="VVE42_000270"
     CDS             complement(23596..25584)
                     /locus_tag="VVE42_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU3072539.1"
                     /translation="MFRLTNKLAVSNLIKNRKLYYPFALAVLLAVTITYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKELGIYGMLGLEKRHL
                     ISMTFKELVLFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQMKVVITVLL
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKKGRFLPLQTILGSISLGIGYYLAL
                     TVKDPLTALTTFFIAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSAESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDKGYKIKEKEVLRYTYFAVANQEGTKLTIFEKGQNRVQPKTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFAKNDGLKGQKALTLNDHQFSVKEEFNKDFIVNHVPNKF
                     NILTTDYNYLVVPDLQAFLDQFPDSAIYNQFYGGMNVDASEEEQLKVAEEYENYLNQF
                     NAQLDTEGSYVYGSNLADASSQMSALFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQTINKQVLTVFFLPLLFAFIHLAFAYHMLSLILKVIGV
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(25586..26344)
                     /locus_tag="VVE42_000271"
     CDS             complement(25586..26344)
                     /locus_tag="VVE42_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3072540.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPSRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVMTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSAALLDVFNEINERGQTILMVTHSTAAASRAKRVLFIK
                     DGILYNQIYRGEKTERQMFQEISDTLTVMASEVN"
     gene            26772..26963
                     /locus_tag="VVE42_000272"
     CDS             26772..26963
                     /locus_tag="VVE42_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3072541.1"
                     /translation="MKHKEHILIGFLYLLSPFIGQLLVEHTHFISTEFTGTAYVICWL
                     SVVISIHHFSKNVLSQQQK"
     gene            complement(27317..27589)
                     /locus_tag="VVE42_000273"
     CDS             complement(27317..27589)
                     /locus_tag="VVE42_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEU3072542.1"
                     /translation="MNSKFYKMRRNRMISGVLAGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVIIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGWFW"
     gene            complement(27648..28097)
                     /locus_tag="VVE42_000274"
     CDS             complement(27648..28097)
                     /locus_tag="VVE42_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEU3072543.1"
                     /translation="MKLTDYVKQVSLEDFGRPFIHHVQWNRRLRSTGGRFFPKDGHLD
                     FNPKVYQELGLDVFRKIVRHELCHYHLYFQGKGYQHKDRDFKELLKAVDGLRFVPSLP
                     NSNSKPIKLYRCQSCQQTYQRKRRIDTKRYRCGLCRGKLLLVNQPED"
     gene            complement(28084..30213)
                     /locus_tag="VVE42_000275"
     CDS             complement(28084..30213)
                     /locus_tag="VVE42_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEU3072544.1"
                     /translation="MDKKYEKISQDLGVTLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEAVLAKIQEQGKLTKELEEAILVAEKLADVEELY
                     LPYKEKRRTKATIAREAGLFPLARLILQNIVDLEKEAEKFVCEGFATGKEALTGAVDI
                     LVEALSEDVTLRSMTYQEVLRHSKLTSQAKDESLDEKQVFQIYYDFSETVGTMQGYRT
                     LALNRGEKLGVLKIGFEHATDRILAFFATRFKVKNAYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVKPASARQIEEAKKDLADLIGQYGVEIIAIGNGTASRESEAFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNVNTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRSQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYAAVKELFKCL
                     DIKDLNEEAQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQD
                     VLDIKDLVVGQKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVAVGD
                     LVSVWVKKIDTEREKVNLSLLAPNETD"
     gene            30257..30340
                     /locus_tag="VVE42_000276"
                     /pseudo
     CDS             30257..30340
                     /locus_tag="VVE42_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            30670..31575
                     /locus_tag="VVE42_000277"
     CDS             30670..31575
                     /locus_tag="VVE42_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEU3072545.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGILFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSILVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSYLSSAKKVFQVFVQAIDEFFDTGRYLVFGCLFASIIQVYVPTRILTSISATPLFA
                     ILLLMLLAFLLSLCSEADAFIGASLLSSFGLAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLAYSLLIGVIL"
     gene            31572..32387
                     /locus_tag="VVE42_000278"
     CDS             31572..32387
                     /locus_tag="VVE42_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEU3072546.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNSRLAKMTSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTDLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQITNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWISAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
     gene            complement(32517..33125)
                     /locus_tag="VVE42_000279"
     CDS             complement(32517..33125)
                     /locus_tag="VVE42_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU3072547.1"
                     /translation="MKKYFISGLGSNAYHSEEESICLIGHSLGGDLARYFASEFEEVK
                     KLILLDGGYLDLDKILPLDTELEETKNYIKSQIISDLDILTSKEKSEAKHWSENMEKA
                     VRQSYHWNVEYNRYELAINYENIEAILRLRRKIQAFKREVGDTLFISPRYPNEATWRE
                     EALKELPDYFDTIFLENFGHELYTQAPKEIASLMNEWLAYFL"
     gene            complement(33129..33440)
                     /locus_tag="VVE42_000280"
     CDS             complement(33129..33440)
                     /locus_tag="VVE42_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3072548.1"
                     /translation="MELKDFTEKEQEMIKKRLTMSNISDKETTEKILALVPQDLIKRI
                     PFFVRKHATTRTIKRISIEYPELYAVAQTSGEIPEKEREELRQIITTIFEQKMNKHSI
                     K"
BASE COUNT         9859 a         7473 c         6159 g        10050 t
ORIGIN      
        1 ttttctgatt ttagttctga aagttccaaa aggtgcaacc gtatttacat gaataaactt
       61 atacacttcc caagtcgctg ttttagtcgc ttcatcagcc cactttctca tatgcggttc
      121 aaataattct tcctcactca acgaatcaat cattgcagag atagaattaa tattttcatt
      181 taattttgct ttcaactctt gcagagataa atgagcgtag gtatctgtga accactgata
      241 taattcacca agttgattcc atttaaattt atccgatggt gtttttactt gaagcccctt
      301 tctttcatct tcttcccatt taagaaccaa gttggtccaa ccaacctgat aagcaaggtt
      361 ttctgctgga gttctgtcaa cttcatcagc tctcttatct tttaaatttt ctggaatatt
      421 atcaaattct aaaatatatt tctcaaatgt tttctctatc tcagctttta gttcttcttt
      481 attttcgtat gttcgcaaaa aatcaccttc tttctgattt atcaatcctt tgatttaaaa
      541 tgaccttatt tttctattta taccttctaa gaacgctata actgctgctc caaccatcgg
      601 tattccgtat gggcttaata aaaaacttaa taccaaagct tctataccaa gtgatacatc
      661 tttatattat catcactaat taagttaaac agaaacagac agaattatta catcctatct
      721 ccctttattg tttttcttcc cagtcgtggt aggcagcgta gagttgactc ttattccact
      781 ggtaaatctt agctacttcc ttaatagctt gatttttctt cataccttgc tggatacgag
      841 cttggatttc taagaacaag tcttcctcgt ctttttcttc cacatcctga ctggcacctt
      901 caacaatcag aagacattca cccttgagag acgtttcaga gatgctttcc agcaattcag
      961 aaattgtacc tctttggtat tcttcataga ttttggtcaa ttccctgacc aagataacgg
     1021 agcggtcacc gtagacttct aacatatttt ccaacgtgtc tgctacacga tgaggtgatt
     1081 cataaaaaat ctgtgtttca ggataatctt ttttagagcc aaaaaattgc ttctgttgac
     1141 ctgattttct cggtaaaaaa ccgtaaaaga tatgtggctg tggcgctaaa ccactggcaa
     1201 tcaaggcaga aattcctgca gaggcacctg gaactgtaac aactgcaatt tcttcctcaa
     1261 tagctgcctt aaccaaatca tgaccagggt ctgaaatgct aggcaaaccg gcatcagaga
     1321 cctgagcaat actttgccct gctttcaaga aaccaatcaa atcaggaatt ttttccttgg
     1381 cattgtgctc atgaaaactg atctgcttgg tggaaatgtc aaaatgcttg agcaaaagac
     1441 ctgtattgcg cgtatcctca gcagcaatcc agtccacttc tttcaaggtc tggatagcac
     1501 gaaaagtcat atcatctaga ttgccaatcg gcgttgccac tagatacagc ttgccatagg
     1561 gagactgccc cttaaaactt ttttgaattt gcatgcctac tccctgtata acaactcatc
     1621 acagaacata cattcttcgt cctgctctcg acgttgtcca taaaaatcat tacatacgtg
     1681 aaatccatca cggtaaatgc gacggacact ttcacgaaca tgcttggcct tgacaggagc
     1741 atctgcttcc acctcaccca agcgttctcg caacttagaa ttttccaaac gaagagctgt
     1801 attttcctct accaggctct tgagattttt cttgatggct tccacatcgg ccaaggttac
     1861 caataactgt tgggaaaaat catccagcgc gtcaaataat tcttttttgt ccataaaaca
     1921 gccctttcct ttctttatca ttcatttttg agtttatatt tctttcaaga ccagatattc
     1981 catggcattt tgaaagctga cattagcttg ccacattttt ctagcttcta gtaaatcttg
     2041 tagaatcact cttactctta cctgcaagag gtcctgccca cagagaactt caagaatccg
     2101 taaaacctga tcctgttttt ccttatcatc tgccaagttg gctaatttgg caacctgtag
     2161 ataactttct tttttcttag ctactaacca agtcagcagg cgttcacttt catcgaccaa
     2221 ggtccaaaaa cttgcctgat tagccaactt ttctgcttca gctcgcgatt gactaaactt
     2281 agctaaaaga gtcgcttttt tcttaacaag tcccatttgt tctaagagta agataagttt
     2341 ttcttcttgc tttttaaagt ggaagatctg agtccgactt cggattgtcg gtaacatctt
     2401 ttcctcatcg ctagtcaaga agaaaatata aacttcactc tggggttctt cgatgacctt
     2461 gagcagagaa ttggctgcgt tgggatgcat tttatccgct tgctcgatga taaagacctg
     2521 ttgctggctt tcaatccctg cttgagaaaa ctgtcccacc aattcccgaa tgcgttctgt
     2581 cttgatgacc tgattgactg gcttaatcaa ggtgacatct ggaaactctt cctgttcaat
     2641 cagcttgcaa cttcggcatt tctcacatgg taagacgcca actttatccg tacaaaagag
     2701 gctcttagct aaaaattgcg ctatttccaa gcttccaaag aaacctgaaa agagataggc
     2761 gtgattgagc tggtcttgtt ctaagatacg gacaaaacgg tcaaactgag ctggttgcca
     2821 agcctttagt tgatcttgtt tcatttggcc aagcccattc cgtcaaacaa gacagccttg
     2881 gtagtttcca caacttgctc caaagggaga ctagcatcaa tcttgacaat gcgatttccc
     2941 tctttatcca gaagagaaag gtagccttga cgaacttttt tatgcaagtc caacccttcc
     3001 aaatccaaac gattaacctc gcggtcacta ttagcagcaa tacgagccag cccttcttcc
     3061 acctcgatgt caaaatagag tgtcaaatcg ggtttgaggc catctgtcgc aaactgattg
     3121 agccagtcaa tggcttcaat atctaagcca cgaccaaatc cctgataggc aacagaacta
     3181 tcgataaaac gatccatgat gaccaacttg ccagcttcaa gggctggaag aactttttcc
     3241 accaaatgct gtctgcgact ggcaatatag agaagtagct ctgttttagc atccatctga
     3301 gtatgacttg gatccaaaat cacttcccga atcttctccc caatcaagac tccgccaggt
     3361 tcacgggtcg tcaacacctc tactcctttt tcctctaaaa ttggtagcag agcctctaaa
     3421 acactggtct tgcctgctcc ctctggtccc tcaagagaga ctaaaaatcc ttttgacatg
     3481 tctaactcat ttctttttta ctacttctat tctatcaaaa aaataggggt ttgtgacaat
     3541 atttttgtct tctcatttca tactcaatga aaatcaaaga gcaaactagg aagctagccg
     3601 caggctgtac ttgagtacgg caaggtgaag ctgacgcagt ttgaatttga ttttcgaaga
     3661 gtatcaacca ccataaaaga ggcagataaa gctacctctt atttacctag ttcttgtgtt
     3721 cgtttatagg cttgatgaac tgcctccatg actgtgcctc gaaaagcatg cgcttctagg
     3781 cttgctacgc cagcaatagt cgaaccgcct ggactacaaa cttgatcttt caaaactcca
     3841 ggatgttgct ggctttctag gaccaattgc ccagctccta ccacagtttg ggctgccatt
     3901 ttcaaggctg tttctcgtga taatcccgtc tgaacacctg catctgccaa ggcttcaata
     3961 aagagataga caaaggctgg tccacagcct gcaagacctg tcgctgcatt gattaagctt
     4021 tctcctaatt caaccaagag accagccttg gataaaagct gacaaaagag ctcactgtcc
     4081 tcagccctgc aattaggaga caaggcataa ctaatcactc cttgcccgat agaagcaggg
     4141 gtattaggca tcatacgaat aattcggtgt tgacttggga taagacttgc tagtttttct
     4201 aaggtcaatc cagctgccat cgaaatcaaa agaagacttt ctcttttttc aaggatggtc
     4261 tggtattgag aaagcagttc agaaaactga gcaggcttaa ctcctagaaa aatcacatct
     4321 gcttctgcaa acatttcttc attgctggaa gcctgaccac caaagtctgc aatgaaagca
     4381 tctaccttag cttgactacg attggcaagg agaatctcat ctgacgtcct agtctgcaag
     4441 acagattttg ccaagctagc acccatattc cccaaaccga taaatccaat cttcatctct
     4501 tactccctta tctgcccatc accagcaacc acatacttgt agctggtcaa ctctttcaag
     4561 cccattggac cacgcgcgtg caatttctga gtagaaatcc ccatttcaca accaagacca
     4621 aattgtcctc catctgtgaa acgagttgag gcattaacat acaccgctgc agagtccact
     4681 tgatctgtaa agtatgctgc agcttcagca ttttccgtca caatagcatc cgaatgatgg
     4741 gtgctgtggg attcaatgtg cgcaaccgct tcttctaaac tgctcacaac cttaacagca
     4801 aggacatagt ctaaaaactc ggtgtcaaag tcttgggtct cagctgcttg acctgaaaca
     4861 aactggcttg ctttgctatc caagcggaat tgaattggtt ccagtccagc ttccttacgc
     4921 tctgcaacca gcatttgatc caagcgagga aggatgcttg ctgccttgtt ttcatgaacc
     4981 agcagaacct ccatggcatt acaaacagaa ggacgactgg ttttagcatt gttgatgata
     5041 gacagcgcct tgtcttcgtc tgcatcctta tccacataga catggacaat cccagtccct
     5101 gtctcgataa caggtacaat cgcattctca accactgcat tgatcaagcc agctcctcca
     5161 cgaggaatga gaaggtctag atagcccttg gccttcatca tagcataact actttcacgg
     5221 ctagtatcct ccaccagttg aatcacattt ggatgaatag tagtcgtctc caagcccttc
     5281 ttcaaggctg tgacaatggc atgggttgtt tgataggcat ccttaccact acgaagaaca
     5341 accgcatttc cactcttaag agtcaaagca gccgcatcag acgtcacatt tggacggctt
     5401 tcatagataa taccgatgac acccatagct acacgttttt ttgtgataac caaaccattt
     5461 tcaagctgac ttgtttctaa aacttcaccg attggatctg gtaaggcaac cacttcacga
     5521 attcctcttg ccatcgcttc tatacgatct gcatccaaat aaagacgatc caacatcaca
     5581 tctgagattt tccccttagc cgctgccata tcgagggcat tagccgctaa aatttcctca
     5641 gtagcagcca ctaagtgatc agccatggct agcaaggctt ggtttttcac ttcttcacta
     5701 gctgtgttaa tcgatttttt aacagcctgt acctgttcaa attgttcttg tctactcacc
     5761 atagtttacc tctaaaattc tgtaaaaagt agttggattt caggagtaat ggaaatccag
     5821 tcgtcacggt aaatcaagac acccttggct ttttgagaac gcaacatatc ctccaaagca
     5881 gatgctccaa attgcacgcg tccttttcca agtgattttc cactttcctt gtcaaatact
     5941 gtcacgatat caccgtaaga aaaggttcct tctgcttcaa cgataccaga taagagaaga
     6001 ctctttccat gttgagagag agcttccgca gcccctttat caacccaaat agaaccttga
     6061 ctctgagcat agaaggcaag ccattgtttc tgggtacgaa gccccttctc ttgagcaaca
     6121 aagtaagaac catcctcggt ctcctctgcc gcctcaatca tggaatctga tttcaaggat
     6181 gagcagatat aaacaggaac tcctgattcc gtcgcgatag ttgcagcctt gattttggtt
     6241 aacatacccc cagttccgtt tgacgaacct gctccaccag ccatatcaat aatctcacga
     6301 ttgatggtct cgattctctc caagcgtttg gctcttggat ctgaattagg atttccagta
     6361 tagagaccgt ccacatctgt caagaaaact aaaaggtctg cttggaccat cgccgctact
     6421 tgagcactta gagtgtcatt gtccccaacc ttgagctcat caataacgac actatcattc
     6481 tcattgatga taggaattgc cccacggttg agcaaaaccg acaaagcctg atgggcattt
     6541 ttataacgac gcttatccac aaagtcatct tgggtcagca agatttgtgc agaaacgatt
     6601 tgacgcaaga gaagattggt cgtatattct tccaacaaaa gtccctgccc taccgctgct
     6661 gaagcctgtt tatcagcaat tttagtcgga cgctttttaa atcctaaggc tccaaaacca
     6721 gccgcaatgg cacctgaaga caccaaaatc aactcatgac cagcctcgtg cagcatagcc
     6781 aactgctggg taatatcctt taccttacta cgtgataaac ttccatcctc atttgtcaga
     6841 gaagaagtac ccaccttaaa gacaatccgt ttgtatttca tagtctcact cctattattc
     6901 atatttatcc attataacat gaataacagg aaatagaaaa gagttgatag gaaaaagaag
     6961 gccctaaaac cttcttttct actactgttc tgattccgat ttgtttacac tcgtttcaga
     7021 gctttgtgaa gttgaggatg tcaaatcttg actagttgaa gaatttgtaa cactttcatg
     7081 ttgactcgtt tctttatttt caagatcctt tttcactact tctttggatt cttttatttc
     7141 tgaatgactt gtagttttaa ttgtagcaga tttctcgatc ggaacatcca ctaaccaggc
     7201 accacttgaa tccacagtat agccttctgg tgtcttacca ttcacaagta attgaccatt
     7261 ttctttcaag aagtaccatt tatccttgtc tttaatccaa cctatagctc gtgaaccatc
     7321 tgtcttgtag aaataccaat catttgcctt tttaagccag ccttgcttca tagctcctga
     7381 ttcttctaga tagtaatgat gtccagcaac ttctatctca cctgtcttca tgataccagt
     7441 agaatccata tagtaccagg cttctttatc ttttacccag cccgttttca tttcccctga
     7501 tggagcaaag taataccatt gccccttata ttgaagccaa cctgtcttca tagagccatc
     7561 aacatccaaa taataccact gctctttatc ttgaatccaa ccaatttcca tctggttttc
     7621 tttgttaaaa taataccaga ttccagcgat tttcttccaa tttttagcag caacaccaga
     7681 gtctgtcaaa tagaaccaac gattgttcca ttttttccat tgattctgta ataagatacc
     7741 cgtttggtta aagtaatacc attcaccctt aatttcattc caaccgacag catattctcc
     7801 tgtagaatca ggcgcttgat accaccagtt tccatatgaa ctcttatgcc aaccagcttg
     7861 aaaacttgga atcggcttgt aggaagttga aatgtttact aaaccgtctt gtcgaatatc
     7921 aaaaactgtt gcatcatagt ctttgctggc tgcgttgatt ctctcaattc ctcgttcttt
     7981 gagccaatta acatactcac tatcaacacc atttttccaa ggtagactat ccgaagtttg
     8041 aacaatcaaa ctcggactca aatttttaat gaaatccttg gtatttgatt tgttggtatc
     8101 atggtgatga ttaaacttca tcaaatcaac ttttccaatg agaggaccat acttgtcttc
     8161 tgctccatga acattatcta aatcgccccc aaggtaaatt ttcttgccat tgactttcac
     8221 cacgctaatc aaggaattgg aattgtcatc ccaaattttc tttaattcac ccgatgaatc
     8281 agtttcattt tcataattat agagctgaat atccatgtcc ccaaactgaa aatgagcatc
     8341 cccttgtgtg atattttgaa taactgaaac acctttttct gcagcagtct gtaaaacctt
     8401 atcatagcca tacagattat cccatagacg ttcagaatta gtaatacgac tatcactata
     8461 tttcttaaga tagactcggt caactggata ggtagacagt aattcatcaa catttccaat
     8521 atgatcactg tgggtatggg tcaccaaaat aaaatcaagt ttttggacac ccaattcctt
     8581 caaacgacga aagacacggt ctgttagaac atgcttataa gacgtttcaa ttccttctct
     8641 ccatggatag cgagaatcac ttccatctgg gaaatcataa tcttctcctg tatccaccat
     8701 ggcaaaatgt ccattgcttt caagaataat cgcatcactg ccaccttctt gaacattgat
     8761 aaagtggatt ttatttcctg aactttcttg agcctgaaca ttcccatacc atgacaaacc
     8821 taaaaaagcg cctacaagtg ctaaactagt taatttcttt ttcattctta ctttctcaat
     8881 ctctccaagg tttccttaaa aatctctggg atatctgctt taaattccaa ggtcttacct
     8941 gttctcggat gagtaaaacc taaagtcttg gcatgaagaa attgtccatg tcctttcaaa
     9001 gtcttgcgag gaccatagac ctcatcacca gcgactgaat ggccgatata agccatgtgg
     9061 acacggattt gatgagtgcg ccctgtctcc agttgcaact ctactaagct ataatcgcca
     9121 aagcgttcca agacgtgaaa acgcgtcact gcaggcttcc ctttagcagt tacagcctgt
     9181 ttcttacggt ctttttcact ccggccaatc ggcgcttcaa ttacaccacg atcattaggc
     9241 agatttccat gaacaatcgc ccaatatttg cggagagact ttttatcttt gagttcttgg
     9301 gcaagtacta gatgcgcatc atcgttttta gcaatcatga gaagacctga cgtatcctta
     9361 tcaatacggt gaacaatccc tggacgcaga accccattga tacccgacaa gtccttaata
     9421 tgatacatga gggcatttac tagggttcca ctggtatgac cagcactcgg gtgcacaacc
     9481 attccctgag gtttgttaac gacagccaca tcctcatctt ggtagactat ttctagcgga
     9541 agatcctcag ccacatactc taatacctct ggttctggca catggtaagt gacgacatca
     9601 ccctcttgga ctgtgtattt agctttcttg acttgaccat tgaccaagac ctggcctgat
     9661 ttaatttgtt cattcgcgag actacgtgat aattctgaca aatctgacaa agccttatcc
     9721 aaacgcagac caccagtttc aattttaatt tccatttatt tcctctttta gcattgcaat
     9781 caataaaata atcactccaa ccgtcagata gctatctgcc acattgaaaa ttgcaaagtt
     9841 gataaagtca aggtggaaca tatccacaac aaagccctga ctggccctgt caataaagtt
     9901 tccaagacca cccgcgatta tcaaagtcaa acccaagacc atccagaatg agtcctccat
     9961 gtgtttatgt aaataccaaa tggcacctat cacgacaacc agagtaatga cagcgaataa
    10021 cagctgctga tcttgtaaga tagaaaaggc tgcacctcga ttttgcaggt aggtcaagct
    10081 aacgaaattg ggaatccagg agcgcacttc acccagtgga atctgctgga cgatatagga
    10141 tttgaccaac tgatccagcc caatcaaaag cagtacaatg actgccacta ttgctctttt
    10201 tttcatgatt tcctcttttg atcaaaatat tcttgcatga cttctacgaa gagagtccca
    10261 gcttgactaa gctccacttc ttcacgttta acatagacca tgcggttatc taggttatcc
    10321 ttgagacgaa taactgtaat gccattaaca ctgtcactat ctaaaaatcc agaacctgtc
    10381 gcataggcgt ccgtccgctc caaaatacca ttcaaggtgg cacggtctgt cacattaaac
    10441 atctgtgagc tagcgctggt atcaacaaag ttctctgaat aataaaggta ctcgtctttc
    10501 tcttgagtga aacgaaccgt tggtaaatcc gctaaatcct ccatgactaa ttcctctttc
    10561 tgggctaaag gatgaccctc acagagataa atatgggtat ggaaaggaat caattcgatg
    10621 acctccagac ctaacttttc aacccgttgc ataatcccct ttttattttg attgttgagg
    10681 tagataatcc caatctcact atgcccttgc gccacttcat ctaatatttg aacagtagtt
    10741 gattcaaaaa tacggaagtt cttatagtca ggatagcgct ctgaaaaggc cgtaatagtt
    10801 ggtggtaaga agtcatagtg ctggctagca acggaaaatt catctttttc ttcttcagga
    10861 ttggcatact gattttgaaa aatatcaaat cctttaacca attcttgcgc tttttcataa
    10921 aattccatcc cacgacgggt caagaaagtc cctgagctgg tccgacgaaa aatcttaaag
    10981 cccaactctt tttccaaatc acgaacagaa atagacagac tcggctgact aacatacatc
    11041 ttttcagcag cttcacgaaa agtaccacta ttggcaatag ccacaacata gcgtaattgt
    11101 tgaatgttca tcttctaccc ccaacttctt tatcttttca ttataccata ttttagaagt
    11161 tttccaaaaa ggaaaaaaga acatcctatt cttcttaact atcttcacta tctgcctttc
    11221 tcacgccaat cttattttca aaatcaattc aaaaaaataa gtggctacac accacttcag
    11281 tatcggaaag aaaaacgttg actatttgtg aaaaaagaaa atgccggaaa attccgacat
    11341 ttttttagta agctaacttc ctgaaaatag gagtaaccaa acatcccaga aggcagtgat
    11401 attgatgaga atatgaacta gtattggata gtagagattt ttagtcatgc gatacaatag
    11461 agctaaaata agacctccac tagcataaat gaaaaaagca agaggagtta aagcaaaatt
    11521 gatatgaata taaccgaaaa taatagcaga aagcaaaaca tctccgtacc aaggtgagtt
    11581 tttgaaaaag gttgtcataa gcacacctcg ataaatcaat tcctcagcaa taggggcgat
    11641 gaagcaaacg atgagcaaga aataagggaa ctcctgtctc cccatcattt ctaccgtttc
    11701 attcaaagaa atctgatttg aagacaggga tatgaaatac gaaaagagga agtcagacat
    11761 atatgaaatg atgtagccca gtaaaaggta gatgaagtac ctcagctgcc acttgaaatg
    11821 aaaaattttc tttcctgcaa aaaccagtag atagatgacc gctaagaaaa gaactccgct
    11881 ctccatcaaa agcagaatct gaaaaaattc acgacttgct ggtagatagg gctttgcgag
    11941 gtgattgaaa actctccaaa accaagttga tttgtaaaaa attaaggaca atgctagtaa
    12001 aatttgaata gcccgttttt tcatattaaa ttctctgctt tctccccttg tttcttcata
    12061 ttactaataa attttactta aaatctcgca gcacttcctt tgcaaagatg attgcctcct
    12121 ccaatatatc ctaaatcata ggtgcctctg ggacaaaatc catgaaccgg cagagttaat
    12181 actggttcta tttcccaatc gtacctacca ttaggatttt taccatatgg atctgctcca
    12241 ccttgtatca gctctaattc ttcatttgtt agatttatat tttttgtcat taaaatcatg
    12301 atatatttcc tccattattc ttcagattag ttgagtaatc tgatactcat acctacttac
    12361 aaaaaaacta ttatattaag ttggtttttt aatttgtcta gttgcaacat tgacaaacat
    12421 agtatagcat atctttgcga aatatcctct tcaaatcatg aattgtcatc aaaacatctt
    12481 aaactataaa atcaattagt ctcaagcttt ctatcaattt cttctcaaaa atatgctata
    12541 ataatagcaa aagataaaga aggaagacct atgattaaac tactagcctt ggatatggac
    12601 ggcaccctcc tcaatgaagc caaggaaatc ccacaagctc acattactgc tattcacaaa
    12661 gccattgaaa aaggtgtcaa actggttctc tgtacgggtc gccccctttt cggtgtcctc
    12721 ccctactaca aaaaactgga gcttgacctc cagaacgaat atattattgt taacaacggt
    12781 tgttcaactc accagaccag tgattggagt ctagttgact ggaaagaact gagtccagct
    12841 gacatcgaat acctctatga ccttgctgaa aagagtgatg ttcagttgac actttttgac
    12901 gagtcacatt attttgttct cggtggcaag cccaatcaag ttattgaaaa tgatgctaaa
    12961 ctagtatttt cagacctgac tgaaatttct cttgaagaag cgactagtgg aaagttccgg
    13021 atgttccaag gtatgttttt aggaacaaaa gaacaaacag acgattttga gcagcatttt
    13081 gctgaagagc tttgccaacg attcagtgga gttcgttcgc agcctgtcat ttatgaagca
    13141 atgccgcttg gaacgacaaa ggctactgct ctttcacgac tagctgagat tttgaagatt
    13201 gattcctcag agattatggc catgggcgat gctaataacg atatcgaaat gctccagttt
    13261 gcagggcttg ggattgcaat gggaaatgcc agcgattatg tcaaatctct tgcggatgcc
    13321 gttacctcaa gcaacgaaga agacggcgtt gcgcgtgcta ttgagaaata tattctataa
    13381 aaaagaaaag caaatagaca gaagttactg ctatttgctt ttttgctaat attttaaaat
    13441 acggactaat caatagacaa gaatagcgaa tcataaaaat cgatttacta gatgccatag
    13501 tatcttatag ttgctaataa gaagttagtc tagcagaatg aatctctcat ttctcccaca
    13561 ctaatctgta atttgtctat acatttctgg tcttctatct cgaaacaagc cccagtttag
    13621 gcgttcactc gctcccttgt ctaggtcata agtagctaac agaacagctt cttcttgtct
    13681 ttcagctcgt tctagaatag ctcctgtttc atccgtcata aaggaggaac cgtagaagtc
    13741 aagactggaa ctctgtccgc cattttcctc actaggagta acctcctcta aaccataacg
    13801 attggctgcg atgactggaa caatattcgc tgctgcgtgc ccttgcatag tacgttgcca
    13861 gtgaccacaa ctatctgtat ccaaaatcgg ctctgaaccg atggctgttg gataaaagag
    13921 caattctgcc ccattcagag caagacagcg ggctgtttcg gggaaccatt gatcccaaca
    13981 gataccgata ccaatcttag catagcgagt attccagacc ttgaaaccag tgttaccagg
    14041 cgtgaaatag aatttttctt gataataatg gtcatctggt atatgggtct ttcgataaac
    14101 gcccagcact tccccatctg catcaatgac agcaatagag ttatacaaga cattaccatc
    14161 tttttcatag aaactgattg gtaaaacaac ttgtagttcc ttagcaatca ccttaaaatg
    14221 ctgaatggca gtattttccg ctacagattg ggcatactgg tagtagtcat actgacgttc
    14281 ctgacagaaa tagggatgtt caaacaactc gggcaagaga ataatttggg ctccttgctc
    14341 agcagcctga cgtactaaac gctctgcggt ttggatattt gttgccacat ccttagcgca
    14401 ttgcatctga atggttgcaa ctcttacatt tctcatcttt ttctcctatt ctggaatttg
    14461 ttgggtgata cagtggatat tgccaccacc taagagaata tctctggctg gtattccgac
    14521 aactttacgg tctgggaaac acttgctgag gatatctaag gccacttggt cgtttacatc
    14581 ctcaaactgt ggaaccaaga cagccttgtt ggcgatataa aagtttacgt aggaagctgc
    14641 tagtcgttca cctgcgtatc gctcttcttc tccttcttca tagatgtagc ctggcaaatc
    14701 ttcttctgtc acaacttgtc gaactgcagg gataggcaat ttatgaatgg tgaagtgaca
    14761 accttttgca tctgtttcct gttctaagag ttcgagatct gcttttgaca tggcatactg
    14821 gggatcattt tcgtcatctg tccaagccaa aacaacctca gcaggaccaa caaaggcagc
    14881 aacattatcg acgtgttcat tggtttcatc ctgataaata ccataaggaa gccaaataac
    14941 tttttcagca ccaagacttt ctaataatgt gttttcaatc tcctctttag tcaggttagg
    15001 attgcgacca ggactaagca agcaactttc agttacgaga atagttcctt gaccatcgct
    15061 atggattgcg cctccttcca gtacaaaagg tttagcatca tagacaggcc tttccaaggc
    15121 ctcagcaaaa cgactggcta cttggtcatc ctcttcataa tcttgataaa gaccatcata
    15181 ggtgcctccc caagcattaa aggcccaatc cacggctaat ttcttacctt tatcattgac
    15241 gagaatggtt gggccagtat cacgcgccca ggcatcattg gtgggaatgt ctaaataaac
    15301 aaccttgtct ccaagatagg attgggcttc agatagatag gcctgctcca ccaaaagata
    15361 gactctttcc ccttctgcta tggtctcgat gatctgagta aatgctcttt tagcagcctt
    15421 tccttgaaaa ggccatgatc ctggtcgagt cggccatatc atgagggtac catgatgggg
    15481 ttcgtactct gctggcatgt gatagcctaa tttttttgga ctgtccatca tgataatctc
    15541 cctttaaagt cttgatagcc aaaagctttg agtaagctac agtctccctg ttcgtccatg
    15601 agatagagac ttggcaatcc aataccatta aaggtattat ttttgacaaa agaataaatg
    15661 gccatgtctt gaaaataaag tctgtctccg atttggactg gattttcaaa actataatca
    15721 ccaatcacat cgccagtcag acaggtatta gaagaaagtc tgtaggtatg ggctttttcc
    15781 tgtgcctcaa agccatttct caaaggtgga cgatagggca tctcaagtac atcaggcata
    15841 tggcaggtcg cagaggcgtc taaaaccaag atttccatac cgttttctac aatatctaat
    15901 acctcagttg ctaaataacc cgcattaagc gcaatggctt caccaggctc gatatagatt
    15961 tcaagattgt aagtttttcg gatacgcttg atttctgaaa tcagcaaatc cacatcgtaa
    16021 ccttctcttg taatatgatg accaccaccc atattgagcc attttacctc atgtaagtag
    16081 ggaccaaact gttcttctac tgctttcaaa gttgtttgta aatcatctgc tccctgctcg
    16141 caaagggtat gaaaatgaaa tccgtcaacc aaatctagca aatcactcgg aatcttgtct
    16201 atagtaactc caaagcgaga accaggtgca caagggtcat agagcgcgtg atctccttga
    16261 gttgaacact gagggttgag gcgcaaacca acactgacac cagcctctcg acaacgcgga
    16321 ccgtgtttac gcaactgtct ctctgagtta aagacgatat ggtccgttat ctctagcaat
    16381 tcctccaagt ctgcatcctt gaaagcaggc gcaaatacat ggacttcacc aggaaattct
    16441 tcccttgcca atttggcctc atagagtcca ctagctgtcg tacctgatag atactggcta
    16501 atcaagggat aagttttgta gagggaatat gccttctggg caagcaagac cttgcaaccg
    16561 gcctcttctt gtacatattg tagaatgcag caattagctt ctaacttggc caagtcaata
    16621 acataggctg gtgttggtac ttgttctaac ttcattagtc caccatttgt ggattttcaa
    16681 ccacaaccca tgacaaacca tactcattca aagcttccat gaatggatct ggatctaact
    16741 cctcaaggtt atacactcca gcttgtttcc aagttccgtt catgactaat tttgtcccaa
    16801 tcatggctgg aacgcctgtc gtataagaaa tagcttgcga accaacctct gcgtaacatt
    16861 cctgatggtc gcagacattg tagatataga tagtcttttt gacaccgtct ttgacacctg
    16921 taaagataca tccaatattg gttttaccta cagtacgtgg gccaaggcta gcaggatctg
    16981 gaagcaaggc tttcaaaaat tgaattggaa caatttcttg gccgttaaag ttaatggtat
    17041 ccgtacgaag gagtccaaca ttttcaagac atttcatgtg cgtcaagtaa gattgaccaa
    17101 aagtcataaa gaaacgaatg cgtttgacac ctggaatgtt cttggccaat gattcgattt
    17161 cttcatggtg aaggagatac atatcttttt gtccaacttg agggaaatca tactcacgct
    17221 tgatagacat agcttcgact tcgacccatt tcccatcttc ccagtaagaa cctggcgcag
    17281 aaacctcacg gagattaatt tctggattaa agttggttgc aaatggataa ccgtggtcac
    17341 cgccattaca gtctaaaatg tcgatataat ggatttcatc aaaatagtgt ttgagggcat
    17401 aagctgaaaa gacactagtt acacctgggt caaaaccaga accaagaaga gcagtcaagc
    17461 ctgcttcttt gaatttctct tgataagccc actgccatga gtagtcaaag taggctgtaa
    17521 aaccaagttc cttacaacgt ttttcgtaga tagcacgcca ctcagggtct tctgtgtctt
    17581 ctgcttcgta gttggctgta tcgatatagt gaacacctgt tgccaaacaa gcatccataa
    17641 tggttaaatc ttgataaggc agagctacat tcaaaacagc ttctggtttg tagctttcaa
    17701 tcagggcaat cacttcttca accttgtcag catcaagtgc tgcagtttca attttagtac
    17761 ttgttttgcc ttctagcttc gctttcaagt catcgcattt tgacttggta cggctagcaa
    17821 tcataatctc tgtaaatgtt tcgctatctt gacaaatctt tgaaatagca acttgggcaa
    17881 cgcccccaca accaataact agtaaacgac tcattttttt ccttcctctt cttctaaaat
    17941 gtcctcaaca tacttgggca acataaagga tcccacgtgt aagtttgcag tgtagtattc
    18001 tgtgaaaagc tggcgttttt tccaaccttc cttgtcaaaa tctttgacag ggtggtattt
    18061 tttcgatgca aatccaaaca accaatagcc agctgggcta gttggaatat gggcctgata
    18121 aacccgactg attggaaagg cttgattgac cttgcggtgc atgcttcggc aggccgactc
    18181 atcctcgtca aagaagggac tcccatgctg gtaaatcatg atgccgtctt ccttcagagc
    18241 tcgataacta ttgccgtaga attccttggt aaagagtcct tccgtatggc caaatggatc
    18301 tgtcgcatcg ttgatgataa tatcgtaatc atcttcgcag tttcgcaaaa agcgtagccc
    18361 attttggtag taaatggtaa cacgaggatc atctagccct gcagcaaagt ctgggaaata
    18421 ctcacgacag acctcgacca acatctcatc cggttccacg atatcgattt gttccagttc
    18481 aggatagagt gttaatactt gggcaacacc gccgtcacca cccccaataa ccaatacttt
    18541 ctttggattt gggtggacag ccatgggaac gtgaacggtc atttcattgt agacgaaatc
    18601 atccgcatct gagaacaaga catggccatt taaaatcagt attttcccaa aagctggcgt
    18661 atccaagact tcgatatcct gccattcact ttttccagcg taaagttgct tggctgttct
    18721 cagagacaat ttgacatctg gagtatgaac ttcagaaaac cataaatcca tctatttctc
    18781 cttatattat atttagcgca gttagctact gccaaagccc aagtggtata cttggaataa
    18841 gccactgtgg attagttcat ttcctccaat cgcttgacga tttccgaaag ggtctaacca
    18901 cagctttttg tttcaattgg taatgagttt gataacgcat agacgtttta tctcgtctaa
    18961 ggtaacaagg caaaaagttc gtagataact aagtagactt gaaatactat atctggaggt
    19021 actcccatgc tttatgtggg cattgatgtc gctaaaaata aacacgatgt tacagccttg
    19081 aatgtttcag gaaaactgtt cttaaaccac tcactttttc aaataataaa gctggtttta
    19141 aactcttaga tctgtctctt cgacagctca accaagactg tctcatcgct cttgaagata
    19201 ctggacatta tgcctttaat ctactgaact tcctgcatga acaaggatat aaagtttaca
    19261 cctacaatcc tttgctcatc aaggaatttg ccaaatcatt atcacttcgc aagactaaaa
    19321 cggataagaa agacgctcat ggcatcgctc ttaaacttct ttctgacccc aatcgtgaac
    19381 aatttcaaca cgataatcgg caagtagacc taaaaatact ggctagacat attcatcgtc
    19441 tcaagaaaaa cagtctgatt ggaaagtaca atacactcgt tgtcttgata tcatctttcc
    19501 tgagttggat aaaatcgttg gaaagcattc agaatatacc taccaactct tgacacgcta
    19561 ccctaatcct cagaaaagga ttgaggcagg atttgataag ctgatagaaa ttaagcgatt
    19621 gaccgcttct aattcaaggg ctttttgccg tgctctaaaa tcatcgccaa atcatcaaat
    19681 tgaaataaga aatagtttag ataaaactat tgacttttct tatagtttgt ctttctctta
    19741 atgacgttga tatgattgac ctctggatct tccgtccctt ggagggagca accacgttcc
    19801 ttggcgaatt ggatatagtc gacaatttct cgtgtaatgc gttcaccagg agccaagata
    19861 ggaatccctg gaggatagca catgacaaat tctccacaga cctgtccaac agaatcatcc
    19921 aaagttaaac tttttctttc tgaatagaag gcttcttgcg gagacagcac taactcgggc
    19981 tgaatatatt ctcctgctat caaatctttt ccatctcttg aatagagtct cttaatatca
    20041 gccagagcac caaccaagcg ctcgatgtct tggatgcggt cgccgatgga aatataggcc
    20101 aagatattgc cgatatcacc aaactcgatc tgaatgtcgt attcgtctcg caagaggtca
    20161 taaacctcga tacctgttaa gccaataccc tgagtgtaaa ctgacagctt agttacgtca
    20221 aaatcgcaaa ccgaaacacc gtctattagc tcttttgagt aggcatagta gccaccgata
    20281 gcattgattt cacggcgggc atactcagat agctcaatga ctttctcaaa cgactcttta
    20341 ccacgaaggg ccaagttgcg acgtgaaata tccaaactag ccatcaacaa gtaagaggca
    20401 gatgtagact gggtcaggtt aattatctga cgaacgtatt cagaattcat ctgctccccg
    20461 ataagtaaaa tggagctttg ggtcaaactc ccaccagact tatgcatgga aactgctgcc
    20521 atatcagccc ctgcatccat agcagaaatt ggaagtttat cagtgaaatg caaatgcgct
    20581 ccgtgggctt catctactaa aaccatcatg ccagcttcat gagccatttc tgtcaacccc
    20641 tttaggtctg aacagatgcc gtagtaagta ggattgttga ttaggatagc cttagcatct
    20701 ggatggtcct ttatggcctg tgctactcgg tcattttcaa gacctaaagc gataccaatc
    20761 ttaggatcta cactcatctc gatatagatg ggaatggcac cacatagaac caacgcattg
    20821 atagcagatt tatggacatt tcgtggcaga ataatcttat ctcctgcctt gcaggttgcc
    20881 agaatcatag tctgcaccga tgaagttgtt ccaccaatca ttagaaaggc atggctagct
    20941 ccaaaagcat ctgcagccag ctcctctgca tcacgaataa tcgaaatagg atggcctaaa
    21001 ttatccaagg gtttcatcga attgacatca atgcctacac atttttctcc taagagttcg
    21061 acaagttctg gatttccccg tccacgcttg tgacctggaa catcaaaggg aacaatcctt
    21121 ttcttgcgta acttcaccaa ggcctcataa attggggctt ggttttgatc taactctttc
    21181 aaaacatact cctttcaata tttttaggac cttatgaaaa ctaaggaact aaaggttgac
    21241 tcatgaaaaa agagcaggtt ttcacctgct ctgcaatctt ctgacgtgtt tattttggtt
    21301 tctgttgagt atgtctgttc ccgatgtttc ctaactctct agtagcaaat attacgtact
    21361 ttattgatcg tttcacaaga tgacgtgtgc tttcaccaca ctaaaaattt atgaattagg
    21421 acaagtgtcc taacatcaac ttataaaagt aggcttttaa ccctatcaat aatgtggctt
    21481 ttcaaccacg cttcggcatt caaccctgcc tgtccgtagg cattttttac tcgggtttat
    21541 atttgctaga aaacatcatc tcattttcta agctttatta ctatatcatg tttttaagaa
    21601 cttgtaaagt attttttgat aaaaaacaaa aatataatag attgaaacta gaacaataca
    21661 cctctgcttc taaaacattg ttagaaatag attttactgt cctgatcgat ttgttctgtt
    21721 cttgtttcat tttactatat gttcgtaaat gaaagttcta taatgaagtt aaccacccag
    21781 ccctcctaaa aattagatgg ttcaactagc tgatagctct tgtaggcata agaaaacacc
    21841 tctgtgttat acttgttgtt caaccacaaa cacaagaaag gcacagagat gcaagacaat
    21901 tatactacaa aagccaaaca tttgacaatc gatagccgtc gcttaatcga aagatggaaa
    21961 aaagaaggga aatcaaatag agaaattgcc tctctacttg gaaaagctcc tcaaactatc
    22021 cacactgaaa tcaagcgtgg gacagtccga caatgtcttg gaaaagggcg cttcaaagag
    22081 gtttattctg ccgactatgc tcaacagtct tatgaaaaca atcgcaagcg ctcggtcaag
    22141 aaatcaagct tgaccaagga actaaaggaa aagattctcc actatcataa ccaaaaattt
    22201 tcgcctgaaa tgatggttat tgctaaaggg gttaacgtgg gaatttcaac catttactat
    22261 tggattcatc gtggaaaatt ggggttaagc aagcaggatc tgctttaccc tagaaaagga
    22321 aaagcgctta agaaacaggc tagcaccaac tttaaacctg ctggtcaatc catcgaacag
    22381 cggcctgaag ctatcaatct tcgcttggag aatgggcatt atgagattga tacggttcta
    22441 cttacgagag cgaaaaacta ctgcttgctt gtcttgacgg atcgaaagag tagacatcag
    22501 atcatccgat tgattccaaa taaaagtgct gaggtggtca atcaggctct aaaactcatc
    22561 ttaaaacaac acaagattct ttccatcacg gcagataatg gaacggaatt caatcgcttg
    22621 tttgatgtat tttctgagga gcacatctat tatgcgcacc cctatgcctc ttgggaaagg
    22681 ggaactaatg agaatcacaa caggctcatt catagatggt tacctaaggg aaccaagaaa
    22741 atgactccca aagaagtcgc attcatcgaa aagtggatta acaactatcc taaaaaatgc
    22801 ttgaactaca agtcacccag agaagacttc tggatggcta acttgaactt gaaatttagc
    22861 gtcagaaaca aatcacgaaa tcgattcaaa ttttgcaggt cgtttaaata tcctgcgcgc
    22921 aggtgttctt ggtgctaacg atggaattat ttccattgct ggtgtggtta tcggggttgc
    22981 cagtgccacg accaatatct ggattatctt tttatcagga tttgcggcta tcttagctgg
    23041 tgccttttca atggctggtg gagaatatgt atccgtttca actccaaaag ataccgagga
    23101 agctgccgtt tcgcgagaaa aactcttgtt agaccaagat agggaactag ccaaaaaatc
    23161 cctctatgct gcttatatcc aaaatggaga atgcaaaact tctgcccaac tcttgaccaa
    23221 taagatcttt cttaaaaatc cactcaaggc tctggtagag gaaaaatatg ggattgagta
    23281 tgaagaattt accaatcctt ggcacgctgc catttctagc ttcgttgcct ttttccttag
    23341 aagtttgcct ccaatgctgt cagtgaccat attcccaagt gaatatcgca tccctgctac
    23401 tgtccttatt gtcggtgtgg cccttcttct cactggttac actagtgcca gacttggaaa
    23461 ggatccgact agaacagcta tgattcggaa ccttgctatt ggtctcttga ccatgggagt
    23521 taccttcctg ctcgaacaac ttttcagcat ttagaataca agaaatacct cgattttgaa
    23581 gtcgaggtat cttttttaca tttgcacaat cttgcgataa cttcttgaag taatcatgaa
    23641 aatcagcaca taggcgatga ggaagatagc gcagatagac aaggtcacaa tcaacatcat
    23701 agtcgtatcc agtacaccaa tcacttttaa aatcaggcta agcatatggt aggcaaaggc
    23761 gagatgtatg aaggcaaaga gcaaaggaag gaagaaaaca gttaaaacct gtttgttgat
    23821 ggtttgcttg atttgctttt ggtccaaacc gactttctgc aagataataa agcgttcacg
    23881 gtcttcgtag ccttcagaaa tttgtttgta gtagatgacc agaacagttc cgaccataaa
    23941 gataatggat aggaaaatac cgataaagaa gacaccacca aagagggcac tcatctgaga
    24001 actagcatct gctagattgc taccataaac atagctacct tctgtgtcta attgagcatt
    24061 aaattgattg aggtagtttt catactcctc agcgaccttg agttgttctt cttcgctggc
    24121 atctacattc ataccaccgt aaaactgatt atagatagcc gaatctggga attgatccaa
    24181 aaaggcttgt aaatcaggaa caacaaggta attgtaatca gtagtcaaga tattaaactt
    24241 atttggaaca tggttgacaa tgaaatcttt attaaattct tctttgacag aaaattgatg
    24301 atcatttaga gttagagctt tctgtccttt cagtccgtca tttttggcaa agagaccgac
    24361 ctcatttcct gatagagaca gtttttgacc agtcatattt tcataatctt tttggtcaaa
    24421 taccatgaaa actgtttttg gttggacacg gttttgtcct ttttcaaaaa tagttaactt
    24481 ggttccttct tgattcgcaa cagcaaagta agtgtaacga agcacttctt tctctttaat
    24541 cttataacct ttgtcacttg caaactggct caagagtttg tccaaatctt ctttttcaac
    24601 attttgccct gaaaccccaa aatcatgagg atttagaact tttttaaagg attctgcgga
    24661 attgaaaatg cttgtcgctg ctgacatggt taccaaaacc attgttgaca aaatagcgat
    24721 agttgctagt ccaactgcat ttttcttcat acggaaaatc aagttagaaa cagatatgag
    24781 attattaggt tggtaatagt atttcttatt tttctttaag atttgaagga aaacggtaat
    24841 ccctgcattg aacaaaagat aagtaccaaa gataaccagt aaaacagcta tgaagaaggt
    24901 tgttaaggct gtaagaggat cttttaccgt aagggcaaga taatatccaa tccctaaact
    24961 tatggaacca agaatagttt ggagaggaag gaagcgacct tttttctctc cgctagcttt
    25021 ctcacgtgag agctggaggg cattcatacg ggcgattcga agggcattca ggaacatgag
    25081 gcctaggaaa atcaaaccga agacaagaag tactgtaatg acaactttca tctggaaggt
    25141 agcgaccagt tccaccttca atttcatcag tttgagtagg aaagcgaaaa tcaacttgtc
    25201 aaacaaggct ccaataccga ttcctgctcc aacagttaga atcccaaata gcactaactc
    25261 cttaaaggtc atactgatta gatggcgctt ctccaagcct aacatgccat atatacccag
    25321 ttccttggaa cggtttttca tgacaaaact attggcatag aggacgataa tggctgacgc
    25381 aagggtaacg acaaacatac caaatccaag tgttgcttga atggtggttc ctccacggat
    25441 ttccgcaatc ttgggattga aggttaggga gtaaaagaga taggtgatgg tgactgccaa
    25501 gagaacagcc agtgcaaagg gatagtagag tttgcggttt ttaatcaagt tcgataccgc
    25561 taacttattg gttaatcgaa acatactaat tcacctcgct tgccatgaca gtcaaggtat
    25621 cagagatttc ttggaacatc tgacgctctg tcttctctcc acggtagatt tggttgtaaa
    25681 gaatgccgtc tttgataaag agaacacgct tggccctgct agcagctgct gttgagtggg
    25741 ttaccatgag gatggtttgc ccacgctcat tgatttcatt aaagacatca agtaaggctg
    25801 cagatgactt ggaatcaagg gctcctgttg gctcgtccgc aaggagaatt tcaggttctg
    25861 tgatgatggc gcgggctact gctacacgct gtttctgacc accagaaatc tcgtaagggt
    25921 acttctcttg caattggttg atacccagat tttcagctgt catcaccaat ttcttcatca
    25981 tctccgttat aggtcttctt gacaagacaa gcggaagcaa gatattgtcc ttaacagaca
    26041 gagtatctag caagttaaag tcttggaaga caaatcctag cttttcacgc cggaaactag
    26101 aagcctgtga atttttaata gttgctgtat cagttccgtt taggtaaacc tgcccacgac
    26161 ttggtttatc cagcatagct aggatattga gcagagtgga tttaccagaa ccagactcac
    26221 ccatgatagc aacgtagtca cccttttcta cggtgaagtg aatatccttg agggcctcta
    26281 cttggttgcc ctgaaaacga gttttataaa ttttttgaac gtgttttaca tctaaaagtg
    26341 tcatgagaat ctcctttctt aatatcccat caaatgaaat gtagcttgca tcatagtgca
    26401 tcccagctga atgcgctcaa tttctttgtg acttggtttt ctagttttct tatgtaagat
    26461 ttgtttcatg atgttactcc tttgttcttt atgagtctag tttacatcaa aaaaagaccg
    26521 cttgccataa cctaacctta caaaagagac tttaatctta catttttgta agattgggtg
    26581 aaatataagc aaaattactt agaaaatgat ttgatttcct atttttaaag ttgtaaaata
    26641 taagtataaa aaggcttact ttaaaaagga ggaagcggga actaacattt tagtaaggtt
    26701 acctagataa catgccattc aatttaacac ttgcaagtcg ttcaacctat aaggaaagaa
    26761 ggtgcgcttt aatgaaacat aaagaacata tactcatcgg atttctctat ctgctctctc
    26821 cattcatcgg tcagctctta gttgaacaca cacactttat cagtacagaa tttaccggca
    26881 ctgcttatgt tatttgctgg ttatcagttg ttatcagcat ccaccatttt tccaaaaatg
    26941 tattgtctca acagcaaaaa tagattaaaa aactgcgttt aaattactgc ttttatagta
    27001 aaatgaaata agaacaggac aaatcgatca ggacagtcaa atcgatttct aacaattttt
    27061 tagaagtata ggtgtcctat tctagttcca atccactata gtttgtggat gaagctggcg
    27121 aaatattatt aatactattc aaaaatcaaa gtgcaaacta tatcaacttc acattgccga
    27181 ctaaattaca gcctgcggct ggcttcctag tttgcacttt gatttttatt gagtataaaa
    27241 gtaaaaaagc tttaaatcaa gcagtttaga ctattgattt aaagcttttt gcgtattcca
    27301 aatcttttgt aaaacctcac caaaaccagc cttcattatc gtcaatggct tgagcttctt
    27361 tgcgtttacg tgggcctgtt ccgtacattt ctgcttcgat ttcttccttg gttggcatga
    27421 tagaagctag gatgatatag ataatcaccc caagtccaaa gtttgcgact gtaaaaatgg
    27481 caaacagaaa acgtacaagg gtaacatcaa agttccactt gtctgacaga cctgctaaaa
    27541 ctcctgaaat catgcgattt cgtctcattt tataaaactt actgttcatg atatttcctc
    27601 tttctgctag gttagacata gtatatcacg gataggctcg gctttcatca gtcctcaggc
    27661 tgatttacta atagcaactt tcctcgacaa agtccacagc gatagcgttt ggtatcaatc
    27721 ctacgcttac gctgataagt ttgctggcag gattggcaac gatagagctt gattggcttg
    27781 gagttactat tgggcaagga tggtacaaac cgtaatccat ccactgcttt caacagttcc
    27841 ttaaaatccc gatccttgtg ttgatagcct ttcccttgaa aatagaggtg ataatgacag
    27901 agttcatgtc ggacaatttt cctaaaaacg tccaacccca gttcctgata aaccttggga
    27961 ttaaaatcca aatgcccatc tttggggaaa aatcgcccac ctgtcgaacg tagacgccta
    28021 ttccactgga catgatggat aaaaggtctg ccgaagtctt ctagtgaaac ctgcttgacg
    28081 taatcagtca gtttcatttg gagctagaag agacagatta actttttcac gttcagtatc
    28141 aattttctta acccaaacgc tcaccaaatc tccaactgcc accacttgac tagggtgttt
    28201 gataaacttg cgactcatat gggaaatatg gatgagaccg tcctcatgaa ttccgatatc
    28261 aacaaaagca ccgaaatcaa caacgttacg caccactcct tctagctttt gtccaaccac
    28321 taagtccttg atatctagga catcttgacg gagaactgga gcatcaaagg aatcacggaa
    28381 atctcgacct ggtttgagaa gatctgcaat gatatcttta agagtttctg gaccaaggtc
    28441 taactcttgc gccatttcct tgactgaaag cgacttgagt ttgctttggg cctcttcatt
    28501 cagatcctta atatccaagc atttgaagag ttccttaacg gcagcgtaat tttctgggtg
    28561 aactcctgta ttatcaagga tattgctact ttcagggata cggaggaaac cagcagcctg
    28621 ttcaaaagcc ttggccccca aacgaggaac tttcttgatt tgggagcgtg aagtgatttt
    28681 tccctcttcc tcacggtatt tgacgatatt ttcagaaatg gttttattga gtccagctac
    28741 gtgtgaaaga agagctgggc tagctgtatt gacattgaca ccaacttggt taaccactgt
    28801 atcgacaaca aagtccagac tctcagatag tttcttctga ctgacatcgt gttggtattg
    28861 accgacacca attgacttag gatcgatttt gaccaattcc gcaagaggat cttgcaaacg
    28921 acgggcgata gaaatggcag agcgtttttc aacggtcaag tctggaaact cctgacgagc
    28981 aagttcgctg gcagaataga cagaagcacc actttcatta acgataacat agctgacttc
    29041 agggaaatct ttcagaactt ccgctacaaa agcttcactt tcacgactgg ccgttccatt
    29101 tccaatggca ataatctcta caccgtattg accaattaaa tctgctaaat ctttcttggc
    29161 ttcttcgatt tgacgagctg atgctggttt aacaggataa ataacctgag ttgtcagcat
    29221 ttttcctgtt gcatccacga cagctagctt ggcacctgta cgaaaggctg ggtcaaaccc
    29281 aagaaccacg cgccctttca gtggagcaac caagaggaga ttgcgcagat tgtcagaaaa
    29341 aagttggata gctccctctt cagctttctc agttaattct gtccgaatac gacgctcaat
    29401 agcaggcaag acctttttct taacggattg ctgaacaact tcatcaatat aagcattttt
    29461 caccttgaaa cgagtagcaa agaaggcaag aatacggtcc gtcgcatgtt caaaaccgat
    29521 cttcaagaca ccaagtttct ccccacgatt gagagccaag gtacgatagc cttgcatagt
    29581 tccaactgtc tctgaaaaat cataataaat ctgaaaaacc tgcttttcat caagactttc
    29641 atccttggct tgagaagtga gtttagagtg tctcagcact tcctgataag tcatagaacg
    29701 caaggtcaca tcttccgata aggcttcgac caaaatatca actgcaccgg tcaaggcttc
    29761 cttgccagtc gcaaatcctt cacagacgaa cttttcagct tctttctcta agtcaactat
    29821 attctgcaaa atcaaacgag caagaggaaa gagtccagct tcacgggcaa tggttgcctt
    29881 ggtacgacgc ttttccttat aaggaagata gagttcttca acgtctgcta atttttcggc
    29941 aactaagata gcttcttcca attccttggt caacttacct tgttcttgaa tcttagctaa
    30001 gacagcttcc ttacggtcat tgagatttgt cagactttta tccaaatcaa taatagcctt
    30061 aatcgccacc tcatccagac taccagtcat gtccttgcga taacgcgcga taaagggaat
    30121 agtcgcccct tcagctgtca aacttagaac ggtatcaatt tgctttaacg tcactcccaa
    30181 atcctgagag attttttcat attttttatc cataaatcta ttataccaca agctggaagg
    30241 ctttctccaa tccttttcat actcaataaa aatcaaaaag caaactagga agctagccgt
    30301 aggctgctca aaatactgtt ttgaggttgt ggataagact gacgaagtca gctcaaaaca
    30361 ctgttttgag gttgtggata gaactgacga agtcagtaac cgtactacgg taaggcgaag
    30421 ctgacgtggt ttgaagagat tttcgaagag tatcaactat cgaaaacaaa ttaaaaaact
    30481 cccttttccg caaaataata gtataataga ggtagaattt agaatcgagg tacacctatg
    30541 gctgtcaaat ttacaaaacg agacgacttg gacaagatgt ttgaagagtt tgctaaactc
    30601 cctgatttga aacaagttac tttccctgat gacaaagaga aaaaagtcaa agcagaaaag
    30661 aaaaactaga tgactgcttt tcaacaactc ccatctagtg tacttcaaac tggagccatt
    30721 tttctctcca ttatcattga agcccttccc ttcgttctga taggaagcat tgtctcaggg
    30781 ctgattgaag tttatatcac acctgacaag gtttatcatt ttctccctcg aaatcgttgg
    30841 gggagaatct tttttgggac ctttgtcggt atacttttcc cttcttgtga atgtggaatc
    30901 gtccccatca tcaatcgttt tctggaaaaa aaggttccaa gttacacggc agttcctttt
    30961 cttgtgacag cacctgttat caatcccatt gttctttttg cgacctattc tgcctttggc
    31021 aactccttcc atgtcgccct attacgagct ctgggttcca ttcttgtggc tgtaatacta
    31081 ggaatttttc taggattttt ctggcaagaa ccgattcaga aagaaaatcg tctggcttgt
    31141 catgagcatg atttttctta cttgagttct gcaaaaaaag tttttcaagt ctttgtgcag
    31201 gccattgatg aattttttga tacggggcgt tatttggtat ttggctgcct ctttgcttct
    31261 ataatacagg tctacgttcc gactcggatt ctgacctcta tcagtgcgac tcctcttttt
    31321 gccatcctgc tcttgatgct tttggccttt cttctttcgc tctgtagtga ggcggatgcc
    31381 tttataggtg cttctctact ctcgagtttc ggtttggcac cagttctggc ctttctcgtc
    31441 attggcccaa tgctggatat caaaaatatt ctcatgatga aaaattactt gaaagcacga
    31501 tttattagtc acttcattac gattgtgact ctggtcgttt tagcctattc tctcttgatt
    31561 ggagtcatcc tatgattcga tttttagttt tagctggcta ttttgaactg actatttacc
    31621 tccatctgtc gggcaaacta aaccagtaca tcaacatgca ctattcctat ctggcctata
    31681 tttccatggt gctttccttt atcttggcta ttgttcaatt gtatatctgg atgaagcaag
    31741 tcaaaaccca cagtcatctg aacagccgat tggccaagat gacgagtatt tctcttctag
    31801 ctattccact tgtcatcggc ttaactttcc caactgttag cttggattct cagactgttt
    31861 ctgctaaagg ttatcatttc cccctatcgg aaggaacgga tctagccatt cagacaagcg
    31921 aagggacgac aagccaatat ttgaaaccag ataccagttc ttatttttca aaatcagcct
    31981 atgaaaagga aatgcgaacg gcggcggata aatacttatc ccaagatagt attcagatca
    32041 ctaatgaaaa ctatatggaa gtcatggagg ctatctacga ctatccagat gagtttgagg
    32101 gcaagacaat ccagtttaca ggctttgtct ataacgaccc cagtcatgcc aatagtcaat
    32161 ttctgttccg attcggcatt atccactgta tcgcagattc tggtgtctat ggattgctga
    32221 ccaagggcaa tacccggcag tatgaaaaca acacttggat ttcagccaaa ggaaaactgg
    32281 tcaatcacta ccataaagaa ctcaaacaaa accttccaac cttggaaatc gacagcttta
    32341 ccaaagtcga taaaccagaa aatccctatg tatatagagc tttttaagaa aatcaagata
    32401 aaaacgaaca agttctcttc tgaataacag aaaaagagcc tgttcgtttt ttggttatat
    32461 gaaaattagt gacttgtaga ttttcatctt ataccatttc cagcaataca agtagctcat
    32521 agaaaataag cgagccactc attcattaga ctagcgattt ctttaggtgc ttgagtataa
    32581 agctcatggc caaagttttc taaaaaaata gtatcaaaat agtctggcaa ttcttttagg
    32641 gcttcctctc tccatgtagc ttcattagga tatcgaggac taataaacaa ggtatctccc
    32701 acttctctct taaaagcttg tatttttctc cgtaggcgga gtatcgcttc tatattttca
    32761 taatttatag ccaactcata tctattatac tcaacattcc agtgatagga ctgtcttaca
    32821 gctttctcca tattttctga ccaatgcttt gcttcagatt tttctttaga agtaagaata
    32881 tctaagtccg aaataatttg agatttgata taatttttag tttcctctaa ctctgtatcc
    32941 aaaggtaaaa tcttatctaa atctagatag ccaccatcca aaagaatcag tttctttact
    33001 tcttcaaatt ccgatgcgaa ataacgagct aaatcgcctc caagagaatg acctatcaga
    33061 cagatagatt cttcctcgct atgataggca ttgcttccca aaccgctaat aaaatatttt
    33121 ttcattctct acttaatact atgcttattc atcttttgtt caaagatagt tgtgataatc
    33181 tgacgcaatt cttcgcgttc tttttctgga atctcaccac ttgtttgagc tacagcgtag
    33241 agttcagggt attcaattga aatgcgttta atcgtacgtg ttgtagcatg ttttctgaca
    33301 aaaaacggga ttcgcttaat caagtcttgt ggtactagcg ccagaatctt ctcagtagtt
    33361 tctttgtcac taatattaga cattgtaagc cttttcttaa tcatttcctg ttctttttct
    33421 gtaaaatctt ttaattccat tcgattagtc ctcctatttt ctctaagtta aattatgtac
    33481 taatacagat gaaactacaa agaataaact ttaagaaatc ttctcactga taagatttta
    33541 g
//
LOCUS       DAVGJL010000007        33110 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.007, whole genome shotgun sequence.
ACCESSION   DAVGJL010000007 DAVGJL010000000
VERSION     DAVGJL010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33110
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.007"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(233..1795)
                     /gene="guaA"
                     /locus_tag="VVE42_000281"
     CDS             complement(233..1795)
                     /gene="guaA"
                     /locus_tag="VVE42_000281"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEU3072549.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
                     CPYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKK
                     IRETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            1937..2635
                     /locus_tag="VVE42_000282"
     CDS             1937..2635
                     /locus_tag="VVE42_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU3072550.1"
                     /translation="MLPAYIKIHDQIKKDIDEYRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERRVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPEKFIKDFKKEEITSHFF
                     QTLQKHGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQVSYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(2678..3574)
                     /locus_tag="VVE42_000283"
     CDS             complement(2678..3574)
                     /locus_tag="VVE42_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU3072551.1"
                     /translation="MSNSLKGTLLTVVAGIAWGLSGTSGQYLMAHGISALVLTNLRLL
                     IAGGILMLLAYATAKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIIFAIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSALTYALYIILPIALIKKWGSSLVIGVGMVIAGLVALPFTGVLQADIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAIFFAFLIMNEQFYPIDF
                     LGMAMILFAVTLISLKDLFLEK"
     gene            complement(3583..4518)
                     /locus_tag="VVE42_000284"
     CDS             complement(3583..4518)
                     /locus_tag="VVE42_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEU3072552.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYLGQSDPEH
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKFGPVELAVPTVRGVEAGIVHDPKARLLGRHAGSAGLFSTI
                     KDLQIFLEHYLADDFARDLNQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(4515..5240)
                     /locus_tag="VVE42_000285"
     CDS             complement(4515..5240)
                     /locus_tag="VVE42_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HEU3072553.1"
                     /translation="MNVNQIVRIIPTLKANNRKLNETFYIETLGMKALLEESAFLSLG
                     DQTGLEKLVLEEAPSMRTRKVEGRKKLARLIVKVENPLEIEGILSKTDSIHRLYKGQN
                     GYAFEIFSPEDDLILIHAEDDRASLVEVGEKPEFQTDLASISLSKFEISMELHLPTDI
                     ESFLESSEIGASLDFIPAQGQDLTVDNTVTWDLSMLKFLVNELDIASLRQKFESTEYF
                     IPKSEKFFLCKDRNNVELWFEEV"
     gene            complement(5324..5959)
                     /locus_tag="VVE42_000286"
     CDS             complement(5324..5959)
                     /locus_tag="VVE42_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-alkyl-2-acetylglycerophosphocholine esterase"
                     /protein_id="HEU3072554.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKMIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     EALNNLEAIIQSVARDYPLTEIKLLSILPVNEGEEYQQAVYIRSNEKIQNWNQAYQEL
                     ASAYMQVEFVPVFDCLTDQAGQLKKEYTTDGLHLSIAGYQALSKALKDYLY"
     gene            complement(5961..6788)
                     /locus_tag="VVE42_000287"
     CDS             complement(5961..6788)
                     /locus_tag="VVE42_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU3072555.1"
                     /translation="MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQAQTWDDAIVNKALAHFKGRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINALFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(6810..7145)
                     /locus_tag="VVE42_000288"
     CDS             complement(6810..7145)
                     /locus_tag="VVE42_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HEU3072556.1"
                     /translation="MGNFISNQRIESMGDEENAKWTERGVLMDVTIKKKDGKTTIGTA
                     KAHPTWVNRTPKGTFSPEGYPLYHYQTYILEDFIEGGSHRDQLDEATKERIDTAYKEM
                     NEHVGLKWY"
     gene            complement(7146..7862)
                     /locus_tag="VVE42_000289"
                     /pseudo
     CDS             complement(7146..7862)
                     /locus_tag="VVE42_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
     gene            8385..9002
                     /locus_tag="VVE42_000290"
     CDS             8385..9002
                     /locus_tag="VVE42_000290"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU3072557.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRAIAEEVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMELRGGVGLAAPQLDISKRIIAVLVPNPNIVEEGETPQ
                     EAYDLEAIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEK
                     HRIKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            9047..9775
                     /gene="rlmB"
                     /locus_tag="VVE42_000291"
     CDS             9047..9775
                     /gene="rlmB"
                     /locus_tag="VVE42_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEU3072558.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKSLSEITEGAVHQGFVLRVSEFAYSELDYILAKTRQEENPLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRAVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKNEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFRNRL"
     gene            complement(9814..10725)
                     /gene="trxB"
                     /locus_tag="VVE42_000292"
     CDS             complement(9814..10725)
                     /gene="trxB"
                     /locus_tag="VVE42_000292"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEU3072559.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDYGDFKKVMTDDQTYETRT
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQDRAFANEKISFIWDSVVKEIKGENRVESVVFENVKT
                     GQVTEQAFGGVFIYVGLDPLSDFVKELNIQDQAGWIVTDSHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGAIAGQEAYKFITEHS"
     gene            complement(10791..11015)
                     /locus_tag="VVE42_000293"
     CDS             complement(10791..11015)
                     /locus_tag="VVE42_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEU3072560.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTARARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(11093..11836)
                     /locus_tag="VVE42_000294"
     CDS             complement(11093..11836)
                     /locus_tag="VVE42_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU3072561.1"
                     /translation="MIKISNLSKSFSGQTVLDHLNLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDELTSALDPELVGEVEKSIADAAKSGQTMILVSHDMSFVAQVADKILFLDKGKII
                     ESGTPDEIIHTPKEERTKEFFASYKRTYI"
     gene            complement(11836..12636)
                     /locus_tag="VVE42_000295"
     CDS             complement(11836..12636)
                     /locus_tag="VVE42_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU3072562.1"
                     /translation="MNVTTILASDWYQNLMQLIPDGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSAGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDTVQTDVKGDLR"
     gene            complement(12794..13150)
                     /locus_tag="VVE42_000296"
     CDS             complement(12794..13150)
                     /locus_tag="VVE42_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEU3072563.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFKLKQFFNTSGIKYRELGLKDKVGSLSNQEAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(13144..13674)
                     /locus_tag="VVE42_000297"
     CDS             complement(13144..13674)
                     /locus_tag="VVE42_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEU3072564.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGKPQDLSNLLLAPIGTNFEKRVWDYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDRNNRRRSTC"
     gene            complement(13674..14168)
                     /locus_tag="VVE42_000298"
     CDS             complement(13674..14168)
                     /locus_tag="VVE42_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU3072565.1"
                     /translation="MEIRLAFPNEVDAIMQVMEDAKKCLADAGSDQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSPEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKVPVDGERLAYQ
                     KLKK"
     gene            14303..14749
                     /locus_tag="VVE42_000299"
     CDS             14303..14749
                     /locus_tag="VVE42_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEU3072566.1"
                     /translation="MKTTFSYPKWAEIPNIDLYLDQVLLYVNQICAPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTVKLYHQTLDLIDHTQEEVIQ"
     gene            14746..15393
                     /locus_tag="VVE42_000300"
     CDS             14746..15393
                     /locus_tag="VVE42_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEU3072567.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLAIIPAII
                     NQTTPVFWSLMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILAASVLQYIAIVYYM"
     gene            complement(15414..15995)
                     /gene="pdxT"
                     /locus_tag="VVE42_000301"
     CDS             complement(15414..15995)
                     /gene="pdxT"
                     /locus_tag="VVE42_000301"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEU3072568.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEGVEILATVNNQIVA
                     AQEKNMLVSSFHPELTDDVRLHQYFINMCKEKS"
     gene            complement(15996..16871)
                     /gene="pdxS"
                     /locus_tag="VVE42_000302"
     CDS             complement(15996..16871)
                     /gene="pdxS"
                     /locus_tag="VVE42_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEU3072569.1"
                     /translation="MTENRYELNKNLAQMLKSGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVAAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            complement(17023..18402)
                     /locus_tag="VVE42_000303"
     CDS             complement(17023..18402)
                     /locus_tag="VVE42_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU3072570.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKEKLEAKGAKVYMNSPVLSIDYDNKVVTAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALADGKIELFRNGAF
                     LVDKKQETSIPDVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
     gene            18696..19619
                     /locus_tag="VVE42_000304"
     CDS             18696..19619
                     /locus_tag="VVE42_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEU3072571.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGISPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQL
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVDGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVESLEGIEVILIDKEGHLSCSSGIPTL"
     gene            19679..20284
                     /locus_tag="VVE42_000305"
     CDS             19679..20284
                     /locus_tag="VVE42_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU3072572.1"
                     /translation="MLKLIAIVGTNSKRSTNRKLLQYMQKHFTDKAEIELVEIKAIPV
                     FNKPADKQVPAEILEIAAKIEEADGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLSHSLQAFNPSGDLVDLD
                     VIKKLDAIFDDFRIFVKITEKLRNAQELLRKDAEDFDWENL"
     gene            20303..21547
                     /locus_tag="VVE42_000306"
     CDS             20303..21547
                     /locus_tag="VVE42_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU3072573.1"
                     /translation="MKFVGLVGSNYDQSYNRKLLEFIRRYFKLKFELEVLEIDEVPMF
                     NQDEKWDESFQLRYLYNKITRADGVIIATPEHNHTISASLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDNNGNITNEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AITGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGGHGDADATSG
                     ASDSGDAGGHGGDADATAGASY"
     gene            complement(21664..21921)
                     /locus_tag="VVE42_000307"
     CDS             complement(21664..21921)
                     /locus_tag="VVE42_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEU3072574.1"
                     /translation="MDPKKIARINELAKKKKTEGLTPEEKVEQAKLREEYIEGYRRAV
                     RHHIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            complement(21963..23999)
                     /locus_tag="VVE42_000308"
     CDS             complement(21963..23999)
                     /locus_tag="VVE42_000308"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEU3072575.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKENRLSFEAI
                     QTFSTPRRLAVRVTGLSDKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEVGQSVEAIVPGVVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEQEFDLDFLDIKGSRVSRGHRFLGKETKIQSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKKIEAKHGVRIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKAGLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILGAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEDFKPSVESLSRAFNLAEKAEGVATVDSALFENDQEKALAEAVETLVLSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            complement(24211..25128)
                     /gene="glyQ"
                     /locus_tag="VVE42_000309"
     CDS             complement(24211..25128)
                     /gene="glyQ"
                     /locus_tag="VVE42_000309"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEU3072576.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATSPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEISDQEMLLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKRLGYPLLDEETRAKLLAEDAE"
     gene            complement(25498..25824)
                     /locus_tag="VVE42_000310"
                     /pseudo
     CDS             complement(25498..25824)
                     /locus_tag="VVE42_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001822577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
     gene            complement(25869..26258)
                     /locus_tag="VVE42_000311"
     CDS             complement(25869..26258)
                     /locus_tag="VVE42_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEU3072577.1"
                     /translation="MDNLYLVKDDSQLATFRDFVVRNTEKLKDYQSFLKNELAVCDLP
                     QAVIWSDFNAATQIIRESAVPAYTNNRRMVMTPDLAVWKELYLYQLMDYECSEQTQAI
                     ESHYHSLSENFLLQIVGHELAHWSDIF"
     gene            complement(26531..27373)
                     /locus_tag="VVE42_000312"
     CDS             complement(26531..27373)
                     /locus_tag="VVE42_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU3072578.1"
                     /translation="MNTYQLNNGVEIPVLGFGTFKAKDGEEAYRAVLESLKAGYRHID
                     TAAIYQNEESVGQAIKDSGVPREEMFVTTKLWNSQQTYEQTRQALEKSIEKLGLDYLD
                     LYLIHWPNPKPLRENDAWKTRNAEVWRAMEDLYQEGKIRAIGVSNFLPHHLDALLETA
                     TIVPAVNQVRLAPGVYQEEVVAYCREKGILLEAWGPFGQGELFDSKQVQEIAANHGKS
                     VAQIALAWSLAEGFLPLPKSVTTSRIQANLDCFGIELSHEERETLKTTAVQSGAPRVD
                     DVDF"
     gene            complement(27488..28879)
                     /locus_tag="VVE42_000313"
     CDS             complement(27488..28879)
                     /locus_tag="VVE42_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU3072579.1"
                     /translation="MDKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIESLKKEKDDQLSEGNQKEHFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKVEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDASKAKEQLIKELTSFIEDKKIEQDQSEQIVKSFSDQDLSAWNFDYKDS
                     QIILYPSPVVENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKYQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEASILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            complement(29114..29491)
                     /locus_tag="VVE42_000314"
                     /pseudo
     CDS             complement(29114..29491)
                     /locus_tag="VVE42_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
     gene            29724..30701
                     /locus_tag="VVE42_000315"
     CDS             29724..30701
                     /locus_tag="VVE42_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU3072580.1"
                     /translation="MLKLGVIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVTQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYAKYSSKMPDLLTG
                     QTPNVFSNRFAGGALMDLGIYPLYAAVRLFGKANDATYHAQQLDNSIDLNGDGILFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGNQVQLPIQQAPHT
                     MTEEVAAFAHMIQQPDLNLYQTWLYDAGSVHELLYTMRQTAGIRFEAEK"
     gene            30698..31780
                     /locus_tag="VVE42_000316"
     CDS             30698..31780
                     /locus_tag="VVE42_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU3072581.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTPIQTQLFEPLLAGENLLGVSQT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYMSATTKFDQEKIVPNTRTIDLSDQKLDNIQHFYMQVDQRHRVD
                     MLRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILAVSLASDVNVKFRKVILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYIHRAGRTGRMGKEGYVITLVTH
                     PEEIKKLKKFASIREIVLKNQELYIK"
     gene            complement(31844..32891)
                     /locus_tag="VVE42_000317"
     CDS             complement(join(31844..32396,32395..32891))
                     /locus_tag="VVE42_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEU3072582.1"
                     /translation="MKSTKEEIQTIKTLLKDFRTAKYHKRLQIVLFRLMGKSYKEIID
                     LLDCNQTTIWRNVKKYEEFGLDSLLQETRGGRNHAYMTVEEEKAFLARHLKATEAGEF
                     VTIDALFQAYKKELGRSYTRDAFYQLLKRHGWRNITPRPEHPKKADAQTIVASKNKIS
                     IQEGKKKRFKYSRRFRKVCLMYQAEAGFGRISKLGSCWAPIGVGPHIHSHYIREFRYC
                     YGAVDAYTGESFFLIAGRCNTEWMNAFLEELSQAYPDDYLLLVMDNAIWHKSSTLKIP
                     TNIGFAFIPPYTPEMNPIEQVWKEIRKRGFKNKAFRILEDVMNQLQDVIQGLEKEVIK
                     SIVNRRWTRMLFESR"
BASE COUNT         9792 a         7415 c         5955 g         9948 t
ORIGIN      
        1 ggatttttat cgctctgaac atcaaaaaag aaaggacgaa ttttgtcctt tctcgagctt
       61 agctgacttc aacccactac agttgacaaa gagccatttt ttgtccaaat gtaaatcact
      121 tggaactatt attcaattta ttctttttag taaatgttcc cgttttaatc ccagttacag
      181 tttttaatcc tctgtaaccg ttgattttac aaagctttta atttttttgc gattactccc
      241 actcaactgt tgcaggtggt ttacttgtaa tatcgtagac gatacggtta acatgatcca
      301 cttcatttac gatacgtact gagatttttt gaagtacttc ccatggaatt ttggcaaaat
      361 cagcagtcat accatcgata gaagtgatag cacggattgc aatcgtgtag tcatacgtac
      421 gaccgtcacc cataacaccg actgaacgaa cgcctgtgtt aacagtgaag tattgccaaa
      481 tatcgcggtc aagtccagct ttagcgattt cttcacgaag aatagcgtct gattcacgaa
      541 cggtttcaag tttctcttca gtgatttcac ccatgacacg gatagcaagt cctggtcctg
      601 ggaatggttg gcgccatacg atatggtctg gcataccaag ctctgtacca agagcacgaa
      661 cttcatcctt gtaaagagta ttgagtggtt caatcaattc aaactgcata tcttctggaa
      721 gaccacccac gttgtggtgt gacttgatag tttgagctgt atccgtacca gactcgataa
      781 catctgtata taaagtacct tgagcaagga atttcacatc tttgagcttg cttgcttcgt
      841 catcgaatac atagacaaac tcgttaccga tgattttacg tttttgttca ggatcagaaa
      901 cgccagcaag tttgtcaagg aaacgtttag cagcgtctgc tttgacgata ttcaaaccaa
      961 acttaccacc gagcatgtcc ataacttgat cagcttcgcc tttacgaaga agaccgtggt
     1021 ctacgaagat acagatcaat tgatcgccaa tcgctttttg gagaagaacc ccaacgacag
     1081 atgagtcaac accacctgat agaccaagaa ggacacgttt atcaccgacg gtttcacgaa
     1141 tttttttgat ctgcatgtca atgaaattat ccattgacca gtcaccttta gccttacaaa
     1201 tgttaagggc aaagttacga aggatatcat ttccgtatac agaatgacga acttctgggt
     1261 ggaattggat accgtaaatg tgtttatctg ggttttcgat ggctgcgtat gggcagtcag
     1321 ctgatgtacc tgtacgaaca aagtcagcag gaatctcagt aaccgcatca ccatggctca
     1381 tcaaaacagt ctgttcatca ggtgttgatt caaaaagcgc tgatggtgtg tgagttaggg
     1441 ttgattgacc gtattcacga tttccagcat cacctgcagg aacaactttt cctccaagtt
     1501 tatgggtcaa taactgcata ccataacaga ttcccaaaat tggaattccg agttcgaaga
     1561 tttctgggtc aatatcaaat gaaccatctt catatacaga atttggacca cctgataaaa
     1621 taattcctac aggattgact tcacgaactt cagcagctga aattttatgg ctttttagtt
     1681 ctgaaaaaac accaatctca cggatacggc gtgaaatcag ctggttgtac tggctaccat
     1741 agtccaatac gatgattttt tctacatctt gcaaatcagt tgaaatgttg ctcatctttt
     1801 tcctttctca aaagaaatat cttttctcaa tattctatca caaataaggg cgaattacca
     1861 actaaacaag gcgaagtttg actttttcta gtttattggg gtacaataga gaaaagatag
     1921 aaatgaagtg aaaaatatgc tacctgctta tataaaaatc catgatcaga taaaaaagga
     1981 tattgacgag taccgttggg ctattggtga gaggcttccc agtgaaagag atttagctga
     2041 gcagtttgcg gtcagtcgca tgaccctccg ccaagccgta tctctattag tcgaagaagg
     2101 cgtcttagag cgccgtgtag gaagcggcac ctttgtttcc agtactcgag tacaagaaaa
     2161 gatgcgaggg acaaccagtt ttactgaaat tgtcaaatcc caaggtaaag ttccctctag
     2221 ccagctcatt tcctacaaaa aaaccattcc caatgagcaa gaagttgcca agctaggaat
     2281 ttttccaacg gacaatatta tccgaatgga gcgggtccgc tatgccgacc aagttcccct
     2341 agtttatgaa gttgcttcta ttcctgaaaa attcattaag gactttaaaa aagaagaaat
     2401 caccagtcat ttcttccaaa ccttgcaaaa acatggctat cgtatcggca aatctcaaca
     2461 gaccatctat gctcgccttg ctaaagaaaa gattgcccac tatttggaag ttgaaaaagg
     2521 acatgctatt cttggattga cccaggtttc ctacctagaa gatggtactg cttttgaata
     2581 cgtaaaaagt caatatgtag gcgaacgctt tgaattttat cttgaaaata attagattac
     2641 cacgcaaaaa ctctctgtca cggacaaaga gtctttttta tttttctaag aataaatctt
     2701 tcaaagaaat caaagttaca gcaaacaata tcattgccat accaagaaaa tcaatgggat
     2761 aaaattgttc attcattatt aagaaggcaa agaaaatcgc cgatattggc tcaattgaag
     2821 ccaacaagct tgacttgacc ggtcctatca gactggctcc tttaaggaaa gctgtatagg
     2881 caaagacagt cccgataagg ataatgcctg caaacgcaag gagaaaatca agactagtcg
     2941 ggatatcggc ctgtagaacc cctgtaaaag gaagggcgac caaacctgct atgaccattc
     3001 ccacaccaat gaccaagctg ctcccccact ttttaatcaa ggctatgggt aaaatgatat
     3061 acagagcata agtcaaggca gaaaagagac cccagaacag accagcaggt gtcatggata
     3121 actggtccaa ctgcccatgt gttgcgatca ggaaggttcc tccgatggcg aatatgatgg
     3181 aaactatctc tcccagtgtc ggtgccaccc tatccttgat acagctataa attaaaattc
     3241 cgacaggaca aacatactga agcaccgtcg ctgttcccgc attggtctcc tgaatagcag
     3301 acagataggc gaattggttg agaaaaagac caatcagagc aaaaataaga agagacagca
     3361 aactctttct atcctttaaa aagaccagta ttttatcctt tgcagtagca taagccaaga
     3421 gcatgagaat tccaccagcg attaaaagac gcaagttggt caagaccaga gccgaaattc
     3481 cgtgtgccat taggtattgg ccactcgttc ctgacaaccc ccaagcaata ccagccacaa
     3541 ctgttagtaa agtccctttt aaactatttg acatgtctct ccttactctt ctttgcgaat
     3601 caagttcatc acttgattgc ggtctaatat ccattgagct ctctcgtcct tttcataggt
     3661 acgattcgat aggaaaatag tggcttcttg cttctgacga ttccacatga taaaggtacc
     3721 tgtatagccc gtatggtcta gccaatctcc ttccaaattc catgctaaag aacgttcctt
     3781 gtcatccaaa ggagaaaaat tttgatttaa gtctcttgca aaatcatctg ctaaatagtg
     3841 ttctaaaaag atttgtaaat cctttatagt cgaaaataaa ccagcactcc cagcatgtct
     3901 acccaggaga cgagccttgg gatcatgcac tatgcctgcc tctacacctc taactgttgg
     3961 aacagcaagc tcaactggcc caaacttagt ttccgtcatt ccccaaggtt tccagacttg
     4021 atcctttaaa atcacatcca aatcttgatt aaaaattctt tccaaaataa agcccaacag
     4081 caaaaaatgg acatccgaat aaagaaaggc tggctgactt cgtctgttga gatgaaacat
     4141 cgcttccttt aattcagggg ctgttaaaag atcacgatta ggaataaaag gatcaaggtc
     4201 tgttgcatga gtcaagagct ggcgaatagt gatgtctgga taatcactct caggtaaaaa
     4261 atctattacc agtctatcaa tatctaattg acctatttcc cacaagaagg tacaaactgt
     4321 gccaacccca acaaccttac tgacactagc taggtcataa actagtcctg cctcagtctg
     4381 caagccatgc tctgggtcac tctggcctaa atagaactct gtccattgat tgtccttaaa
     4441 atacgcaaaa gaggctccgg gataaattcc tgcctcgatt tgttcttcta tttttttaat
     4501 aatcttggtc cacttcatac ttcttcaaac cacaattcaa cattatttct atctttacaa
     4561 aggaagaatt tttcagactt aggaataaaa tattcagtag actcaaactt ctggcgaaga
     4621 cttgctatgt ctaattcatt gaccaagaac ttgagcatag ataagtccca ggtaaccgta
     4681 ttgtccacag tcaaatcctg cccctgagct ggaataaaat caagggatgc cccaatttca
     4741 gatgattcca agaaactttc gatatcagtt gggagatgta attccataga aatctcaaat
     4801 ttacttaaag aaattgatgc caaatctgtt tgaaattcag gcttttctcc tacttctact
     4861 agacttgctc tgtcatcttc cgcatgaatc aaaatcaaat catcttctgg tgagaaaatt
     4921 tcaaaagcgt agccattttg acctttatat aatcgatgaa tcgaatctgt tttagataag
     4981 attccttcaa tttctaaggg attttccacc ttgacaatca atctagctag tttttttctt
     5041 ccctctacct tacgagtacg catactggga gcttcttcta aaaccagctt ttcaagaccc
     5101 gtttggtcac ctagtgacag aaaggccgat tcttctaaca aggccttcat tccaagggtt
     5161 tcaatataaa atgtttcatt taattttcta ttattagctt ttaaagtagg aataatccgt
     5221 acaatctgat ttacattcat aaattcctcc gtcacttttt attttaaagg attttagaat
     5281 tttttacaag atattatgct caaatttaac attatttagc tatttagtaa agatagtctt
     5341 tcaaggcttt tgacaaagcc tgataaccag caatactgag gtgcagtcca tcagttgtat
     5401 attctttttt gagttggcct gcttggtctg tcaaacaatc aaatactggc acaaattcca
     5461 cctgcatata ggcagatgca agctcttgat aggcttgatt ccagttctga attttttcat
     5521 tcgagcggat atagactgcc tgctggtact cctctccctc attgacaggc aaaatggaaa
     5581 gcaatttaat ctctgtcaat ggataatcgc gagcaacgga ttgaatgata gcttcgagat
     5641 tattgagagc ctcattcaca ggaacatcct ttccgatatc atttgtccca atcagaagaa
     5701 aaattttatc tactgctcca ccatatagat gagcatcaag gttctctagt aacagtcctg
     5761 tctgatagcc acgaattcct cgattgacaa tcatctttga agttccaaat agctcctgta
     5821 gaggataata ctcgacaatg gaatccccaa taaaaagaat gtttggttct acaacagaaa
     5881 tgtgatttag gtgacgatat ttggtttgaa ttttttcttg ttcctttagg agccaatttt
     5941 ctaataactg tactgccact tcattctcct ttttctaacc agttttccaa gacttgataa
     6001 actcctcctt ggctgttggc tggtgctaga gcagtcgcca cagctttggc tttctcatca
     6061 gcattttcca tcgcataggc aattccagcc atttcaagca tttcaacatc attttcacta
     6121 tcaccaaaag ccatgatttc ttgggatttc aagtcccagc gcttgagtaa ttcctccaag
     6181 ccccatgctt tatgaatccc agcttggagg atatcaatgc aaccatagcc actggataca
     6241 gctcggacac ggccatcaaa gagagcattg atttcttcca aaaccgaact caaacgttcc
     6301 tcaccaacaa ccatgctcat cttgagcaca ccaccaaaga ggtcagatgt taattcatcc
     6361 acaaattgca tccgttggta gaatttttca atcatttctg gagtcataaa actttcaaga
     6421 tctgtaaaaa tcgtaccttc cttgacaaaa tcacccttca tccccgttac aacaaactgg
     6481 tcctgacacg ctcgaccctt gaaatgagcc aaagccttgt tgacaatggc gtcatcccat
     6541 gtctgagcct gaatcaattc attgttttca aaaatacgag cgccattagc aacaaccaga
     6601 accactcgat ccaccaaggg actcagtagt tgtctcatgc ggtgaatttc attgcccgtc
     6661 gcaatgacaa aacgaatgcc cctttgatcc aactgatcta aaatcttttc caatcgtggg
     6721 agatcaagct gacctctagc atccagcaag gtcccatcca tatctgtcgc aattacctta
     6781 atcatcattt actttcctct ggattcaagc taataccact tcaatcccac atgttcattc
     6841 atttctttat aggctgtatc aattcgttcc ttagtcgctt catctaactg gtcacgatga
     6901 ctgccaccct ctataaaatc ttccaaaata taagtttggt aatgatacaa gggatatcct
     6961 tctggtgaaa aggttccctt tggtgttcga ttgacccaag taggatgagc tttagctgtt
     7021 ccgatagttg tttttccatc cttcttcttg atggtgacat ccatgagaac accacgttca
     7081 gtccacttag cattctcttc atctcccata gattcaatcc gttgattgga aatgaagttc
     7141 cccatttaat agataatgag tttcttatct ccatcttttt caaccgtttc agatggttca
     7201 acaacgtgag gatgccctcc aaagataata tccgctcccc aatcgatcat cttgtgataa
     7261 agagcttttt gttcttcagt tggttccaat cgatactcaa cacccatctg aagcatgata
     7321 atggtgatat ctgcttcctt ctctgcccgt tcaatttcag ccttcatctt atcttcgttt
     7381 aaatctgaaa gataacgatt atagtcttcc tgagaaatat actgctcaat tccattgaaa
     7441 ccataggaat aggctaacaa tgcaaccttg ataccattca cttccttaat gaccagcgga
     7501 gcctgaacac gtggttcgtg cgtataaact ccgattggag tgattccagc tttctcaata
     7561 atatcggccg ttgaaataac tccctcaatt tgcgaatcca aaatatgatt atgagctaaa
     7621 tccagcacat gataacctgc ctccttaata gcatccataa cttcagcagg agcattaaag
     7681 agaagataac ccgctaaata atgatcctta ttaatggttc cttcaaaatc accaatagct
     7741 aaatctgctt gcttgagcca aggagtcaca tactaaaaat tttcatgaaa gtcataggta
     7801 ccgtcttctt ttttagctga aaagaaaagt ccatcgtggt agagcaaatc cccgtgggcc
     7861 ataattctgg cagttttttc ctcttcctac tcctgagacc tttgcttagt cccctcttga
     7921 gaaatagtat ctcgtttttg actagtcaag ggattccttg gaagctttca aacaacaaga
     7981 ccaagcccat tgataaacca actgctagca agagtatcgc cacaaatccc ttattgctcc
     8041 acttgcgata actcctaaaa agtttaccaa gcccttcata aaacgaaaag ctaaaccacc
     8101 ctgatttcga ttttgtcttc tttgtatctt cattctccct actttcttat gcaagccttt
     8161 tctttttatt atatcacaga taagtatttc tttcacaatt gaattgaact tcccatctat
     8221 tttctataaa tcctaaatgc cataatgctt tcaattcttg tcattttgtg atatcatgta
     8281 gaggtaatga aaggaaatga actaatccac agtggcttat tccaagtata ccacttgggc
     8341 tttggcagta gctaactgca ctaaacataa tataaggaga gaaaatgtct gcaatagaac
     8401 gtattacaaa agctgctcac ttaattgata tgaacgatat tatccgtgaa gggaatccta
     8461 ctctacgcgc gattgctgag gaagtcactt tccccctatc tgaccaggaa atcatcctag
     8521 gcgaaaagat gatgcaattc cttaaacatt cccaagatcc tgtcatggct gaaaaaatgg
     8581 aactccgcgg tggtgttgga ctggctgctc cccagttaga tatctcaaaa cgcattatcg
     8641 ctgttttggt acctaatcct aatattgttg aagaaggcga aactccacag gaagcctacg
     8701 atttggaagc cattatgtac aatccaaaaa tcgtctctca ctctgttcaa gatgctgctc
     8761 ttggcgaagg agaaggttgc ctgtctgttg accgtaacgt gcctggctat gttgttcgcc
     8821 atgcccgcgt tactgttgac tactttgaca aagatggaga aaaacaccgt atcaaactca
     8881 aaggctacaa ctccattgtt gttcagcatg aaattgacca cattaacggt atcatgtttt
     8941 acgatcgcat caatgaaaaa gacccatttg cagttaaaga tggtttactg attcttgaat
     9001 aaagaaaatc ccgttgcaag acggggtttt gtgttataat agaggcatga aaacaaatga
     9061 tattgtctat ggtgtccacg ccgttaccga agccctcctt gcaaatacag gaaacaaact
     9121 ctacctccaa gaagatctcc gaggtaagaa tgttgagaaa gtcaaggaac tagctacaga
     9181 aaagaaggtg tccatttctt ggacatcaaa aaaatctctc tctgagatta ctgaaggtgc
     9241 tgttcatcaa ggttttgttc tacgagtgtc tgaatttgcc tatagcgagc tagattacat
     9301 ccttgcaaaa acacgccaag aagaaaatcc acttctattg attctagatg gtctaaccga
     9361 tccccataat ctgggttcta tcttgcgaac agccgatgcg accaatgttt caggtgtcat
     9421 cattcccaag caccgtgctg tcggagtaac tcctgtcgtt gccaaaacag ccacaggtgc
     9481 tattgaacac gttccaattg cccgagtgac caacctcagt caaaccttag ataaacttaa
     9541 gaatgaaggt ttctggacct ttggaacgga tatgaacggt actccttgcc acaagtggaa
     9601 tacaaaaggg aaaatcgccc tcatcattgg aaatgaagga aaaggtatct ctagcaacat
     9661 aaaaaaacag gtcgatgaaa tgattaccat tccgatgaat ggacatgttc aaagccttaa
     9721 tgctagtgtt gctgcggcca ttctcatgta cgaagttttc cgaaatagac tataaaaaag
     9781 tttccagtca tctgattgga aactttttta tgattaacta tgttctgtaa tgaatttata
     9841 ggcttcttga ccagcgatag ctccatctcc aaccgctgtt gttacttggc gaaggtcttt
     9901 caagcgaaca tctccaactg caaagatacc gtcaactgca gtcttcatgt ggctatctgt
     9961 cacaatccag cctgcctgat cttggatatt caattcttta acaaaatcgc taagagggtc
    10021 caaaccaaca tagataaaga caccaccgaa ggcttgctct gtcacttgac ctgttttcac
    10081 attttcaaaa acgacagatt ctactctgtt ttcacccttg atttccttca ctacagaatc
    10141 ccagataaag ctgatttttt cattcgcaaa ggcgcgatct tgtaaaacct tttgggcacg
    10201 aagttggtca cgacggtgaa caatggtaac agtcttagca aaacgagtca agaagagggc
    10261 ttcttcaaca gctgaatctc caccaccaac taccaataaa tcttggtcac ggaagaaagc
    10321 accatcacac acagcacagt aagaaacacc acgactgttc agttcttctt ctccaggcac
    10381 tcccaaagga cggtgtttag aaccagttgc tacgataact gtacgtgttt catatgtttg
    10441 gtcatcagtc atcactttct taaaatcacc atagtcttcg acattttcaa cataaccata
    10501 aatgtgctca acaccaagat tttcaagtgg ttcaaacatc ttttcagcca attcaggtcc
    10561 actaatatta gcgtatcctg ggtaattttc gatatcagat gtattattca tctgaccacc
    10621 tggcagacca ccttcaatca aagctacttt tagattgctt cgagcagcat acaaggccgc
    10681 agtcatccct gcaggtccag caccgataat aatagtatcg tacatataga ttccttcttt
    10741 cttgttgtaa ctatctttat tctaactctt tcttgtcaat cacgcaagga ttatgccttg
    10801 aaaacaagaa ttaaactcat aaccgcaaag gataatactg ggacaaccaa gactccaatc
    10861 ataatcatat catagagatg ggcttgacga gccctggctg tcttatcaac tcccgacatg
    10921 gctctcagac caatccaaat ccctaaaaaa atcagaacaa ggatggtggt caagatcaaa
    10981 ctctcgaaat ataaagaaaa tagttgcagt agcatgattt ctctcatttc tatctttttt
    11041 aaagagtaaa ctcagctagt ccaactaact gagttttcct ttatctatta tatcaaatat
    11101 aagtccgttt gtaactagcg aagaattctt ttgtccgctc ttctttaggg gtgtggataa
    11161 tctcatccgg agttccagac tcgatgattt tccccttatc taagaagaga attttatccg
    11221 caacttgggc tacaaaggac atgtcatgac tgaccaaaat catggtctga cctgacttag
    11281 cagcatctgc aatagacttt tctacttcac cgaccaattc tgggtcaagg gctgaagtta
    11341 gttcgtctaa gagcaaaaca tctggtttca tagcaagcgc acgcgctagg gcaacccgtt
    11401 gcttctgtcc acctgataaa tggcgaggat aatggttttc acggtccgaa agcccaacct
    11461 tagccaactc ttccttggca atcttagtcg cttcttggtc agataatttc ttgacaacaa
    11521 ccaagccttc tttcacatta tcaagtgctg ttcggcgttc aaacaaatta aactgttgga
    11581 aaaccataga caacttacgg cgtagggcaa ggatttcttc ttgagtgatt ttagaaaaat
    11641 caactgaaaa accatcaatc tgaatagagc cactgtcagg tgtttcaaga taattgagac
    11701 tgcgaagaaa ggttgatttt ccagctcctg aagaaccaat caaggctacg acttctcctt
    11761 tttgaatatc caagttcaga tgatccaaga cagtctgtcc tgaaaaggat ttgcttaaat
    11821 tcgaaatctt aatcattaac gaaggtctcc tttcacatct gtttgcactg tatcaggtgc
    11881 agaaatagcc atttttctct cgatgaaacg accgaggctt tcaattccga tattgactac
    11941 ccaataaaca agggcaacgg agatgaagcg ttcaaaatag cgataatcag ctccacctag
    12001 aatctgagct tgggcaaaga cttccacaac acccgcacta aaagctagag atgttccctt
    12061 ggtcaaaccg atgagggaat taatcaaggt tggagtagct accaccgctg cattaggaat
    12121 aatcactcgt cgataaactt gcgctcgggt catacccaga ctgcgtgccg cctcaatctc
    12181 accaggatta actgagagaa tggctgcacg aatggtttca ctagcataag ctgcctcatt
    12241 aaaggcaaag gcgacaatcg caaaagctgc agctggaatc gcattgatat tgagaccagt
    12301 tccccattgc tgattgaggg ctttcaaagc caaaggaatt ccgtagtagg tcaacatgag
    12361 ttgcaccaaa atcggtgtcc cttttaagaa actaacaaag aaggcctgca agggatataa
    12421 aatcttgaca cgattgatct tcacaatggc aaaaagaagc gccaaaacca agccaaaaag
    12481 ggcaccgcca attgtcaaca taattgttgt tggaagttgt tggacaattc tagggattcc
    12541 atcaaagacc gaacgtaggc taaacagctt gccatccgga atcaattgca tcaagttttg
    12601 gtaccaatct gatgctaaaa tcgttgtaac attcataaaa acatcctctt ctgataatga
    12661 ttcattctac catgcttctg ccgtcaatga aattatttgc tacggtcaga tacagacttt
    12721 gtctacctac tagtttatca tactttgaaa cagggaagtt atatatttta tctatcaaag
    12781 agatagataa aaactatttc agtcccagtt cttcataaga ttttcgataa ccgatttgct
    12841 taacagttcc attttctact aaaatgggcc gttttaacaa cataccgtca cttgctagca
    12901 actcagccgc ttcttggttt gacaaacttc ctaccttatc ttttagccct aattcacggt
    12961 atttgatacc actggtgttg aaaaattgct tcaatttaaa tcctgaggtt tctagccaat
    13021 tcaaaatgac ttcttggcta ggtgtttctt ccacgatatg gacggcttta tagtccacac
    13081 ctaattgatt taattcttgt tttgcttttt tacaagttga acatttgggg tattcgataa
    13141 attctaacat gtgcttctcc ttctattgtt tctatcttta aaatctactc cttcatgctc
    13201 caagagccaa gctttctttt ccactcctgc agcataacct gtcagacgct tgcctgctcc
    13261 caacacacga tgacaaggta ctaggataga ccaaggattg cgtcccactg ctccaccaat
    13321 tgcttgagca gaagccactt gcaggtcttg agcaatttgt ccataggtca ctgtctgacc
    13381 ataaggaatg ccctgtaaat agtcccaaac tctcttttca aaattcgttc cgattggcgc
    13441 caagagcaag ttggataaat cctgaggctt gcctttaaag taatcatcta agcaagcaat
    13501 aactgggtct aaaataggat gactaacaac ttcttctatc gtttcatctc ctagtcccct
    13561 ctcaaaatgc ttctgctcct gaacccaaat tccatacaaa taatggtcat cagctacaag
    13621 tgatagaata ccaattggtg aggagtagag gatttttaca tacttctttt gcattatttc
    13681 tttaattttt gataggccaa gcgttcgcca tctactggca ccttaccaac ttgtttaaaa
    13741 ccaagttttt caaaaatatg ttgcataacc ttgttttcag catgcgtatc tgagcgaaaa
    13801 tcaagataat caaaaccttc aatcaagccc tctaagaagg tttgagcaac tccttttccc
    13861 tgcacatctg ctgccacagc aatacggtga aagactagat actctgactc tccagcttgc
    13921 cagtttccct cataaatagc ttcataggcc tcctctggac tcttggtcac agcagcatag
    13981 gctaacagtt ctccctcttc caaggctacg taggcttgcc ctgagatgat atcatcgata
    14041 ataacatctg catttggata gccattttgc cactggtcac taccagcatc tgctaaacat
    14101 tttttggcat cctccatcac ctgcatgatg gcatctactt catttggaaa agctaaacga
    14161 atttccatct tttttcctca tttctgacta gtttctccca tcatagcata atttaagtat
    14221 ttctacaagc agttaaaact tgactttgtg tttttgttat tttataatct agttatcaaa
    14281 actatataaa aaggagttga aaatgaaaac taccttttcc tacccaaaat gggcagaaat
    14341 tccaaacatt gacctctatc tggaccaggt tttgctctat gtcaatcaga tctgcgcccc
    14401 tatctctcct aataaagaca agggcctaac agcatctatg gtcaacaatt atgtgaaaaa
    14461 tggttacctg acaaagcctg acaaaaaaaa ataccaacgc caacagattg cccgtttgat
    14521 tgctatcaca accctcaagt ctgtattttc tattcaagaa atagcccaga cacttaatac
    14581 tctacaaact caagcaagtt cagaccaact ctacgatgct tttgtggact acatgaacca
    14641 aggaattgac ccagctaacc ctattatcca aaccagctgc caaaccgtta aactctatca
    14701 tcaaactcta gacttaatcg atcatactca agaggaggta atccaatgaa cactagtctt
    14761 aaactcagca aacaactcag ttttggagag gagattgcta atagcgtgac ccatgctgtg
    14821 ggtgcagtca tcatgcttat cttgctgcct atttcatcca tctatagtta tgaagcacac
    14881 ggatttttat cctctatcgg cgtttccatt ttcgtcatca gtctctttct catgttccta
    14941 tcatccacca tttatcactc tatggcctat ggttcgaccc acaaatatgt tttgcgaatc
    15001 attgaccatt ctatgattta cgttgccatt gccggctcat acacgcccgt tgtcttgacc
    15061 ttgatgaata actggtttgg ctatctgatt attgtcatcc aatggggaac gaccatcttt
    15121 ggtattctct ataaaatctt tgctaaaaag gtcaatgaga aatttagcct tgctctttac
    15181 ctgattatgg gctggttggt tctggctatc attcctgcca ttatcaatca aacgacaccc
    15241 gttttctgga gtctcatggt aactggcgga ctctgttata cagttggagc tggattttat
    15301 gccaagaaaa aaccttattt ccacatgatt tggcatctct ttatcctagc tgcgtccgta
    15361 ctccaataca tcgctattgt ttattacatg taaaaaagtt gagaaattca atctcaactt
    15421 ttttctttac acatattgat aaagtactgg tgcaagcgca catcatcagt caattctgga
    15481 tgaaaagaac ttaccaacat atttttttct tgggctgcaa caatttgatt gttcactgtt
    15541 gctagaattt ctacaccctc accaacacta ctgataatcg gaccacggat aaaggtcatt
    15601 ggaatcttgc caactccctt acattctgct tccgtgtaga aacttcctaa ttggcgccca
    15661 taagcattac gctcgaccac catatccata gttcctagat gactctcttt ctgagaagtg
    15721 atttccttag ccagcaaaat taagcccgca caggtcccaa acactggtaa gccagataga
    15781 atagcttctc ggatgggaag tagcatgttc tggtcacgta agagcttgcc catggttgta
    15841 gactcaccac caggcaaaat caaacccgac aagtcactct gatcttgctg aaaatcatct
    15901 agatttctga gttctacact ctcgacacct aattgatcta gcacttttgc atgttctgca
    15961 aaggcccctt gcaaggccaa tattccgatt ttcatctatt ttcctcgttc agccatgagg
    16021 atttggattt cattttcatt aataccaacc atggcttctc ctaaatcttc agagatttga
    16081 gctaggattt gaggattacg gaagttagtc acagccttaa caatggcact cgctcgttta
    16141 acaggatctc ctgacttgaa aatacctgaa ccgacaaaga ccccctctgc ccctagttgc
    16201 atcattaacg cagcatctgc tggcgttgca acacctccag cagcgaaatt tacaactggc
    16261 aattttccat gttcatgaac atattggacc aattctacag ggacttgcaa atccttggca
    16321 gcaacataaa gctcgtcctc acgtaagttt tgaatgcggc gaatttcctg attcatcata
    16381 cgcatatgac gaacagcttg gactatatcc cctgtccctg gttctccttt ggtacgaatc
    16441 atggaagcac cttcagcgat acgacgcaag gcttcaccca aatccttagc accacagaca
    16501 aaaggaactt ggaattcttt cttgtccaca tggaaacggt catcagctgg agatagaact
    16561 tcactctcgt cgatataatc aatttcaata gcctctaaaa tctgagcttc aacaaaatgc
    16621 ccgattctga ccttagccat tactggaata ctaaccgctt cttggatttc cttaatcatc
    16681 tttgggtcgc tcatgcggga aactcctcca gctgcacgaa tatcagccgg aattcgttcc
    16741 aaggccatca cagctgccgc accagcagct tctgcgatac gagcctgttc aggattctgc
    16801 acatccataa taacaccact cttgagcatc tgtgccaagt ttttatttag ttcataacga
    16861 ttttcagtca tttgttttat cctcatcatt tgtattggta gctacctttt cattttaagt
    16921 cagaacagaa tgaagcacaa gataaaaaaa ctataattgt atggggacaa attagctaaa
    16981 aactatctga ccttaactta aggccagata gctcattcat ttttattttt cagccgtaag
    17041 ggcagccatt gtgatgtagt tgtatggttt gttgaagtgt ggcaagaaga agaggtctgt
    17101 caatgccaat ttatcaattg tcacatgctc ttggatagca agtgagaaca tgtggattcc
    17161 catgctaatt gcaatatcat gtgaaaccat ttgggcacca agaatttcac ggctatcttt
    17221 gtcaaagaca atcttaattg ctacttcatg gttgtcatgt ttcatgaatt ctggtttttg
    17281 aagatcgtta aagcctgttt cagttgcgtt gtaaccagca gctttcgctt tttcaagagt
    17341 caaaccagtt gaaaccatgt gaagaccgta gattgagata ccatttgaac cttgaacacc
    17401 gattccttcc aattcatgtc cacaagcatt gtaggcacca acgataccag tgcgaacagc
    17461 atttgaagca agagcgatat agcttgtatc tttacgagca ttgtcataaa cagtcgcaca
    17521 gtcaccaaca gcgtaaacgt ctgggataga tgtttcttgt ttcttgtcta caaggaaggc
    17581 accgttgcgg aagagttcga tcttaccatc tgcaagggct gtgtttggac ggaaaccaac
    17641 tgcaaggata accatatcca cgtcaaagct ttctttgtca gtaatcaagc gttcaacttt
    17701 accgtcacct tcgattgctt taacagtttg acctagagcc aagcggatgt tgtgatcttc
    17761 caagttcttc gccatcattt gtgtgaagtc tttgtcatag taaccgttca agacagtatc
    17821 aacgatatca acaaggacaa cttcttttcc aagacgttca aaggcttcag caagttcaac
    17881 accgatgtaa ccaccaccaa caacggcgat acggtcgagg tgttggctct tgtcagaaag
    17941 tttattgata acttcttcag cattttggta caatttcacg aattgtacgt tttcaagagt
    18001 tgctttaaat tcgcggtttc ctttaacaat ttcaacacct tcgattggtg gcaagattgg
    18061 tgtagaacct gtagcgaaaa tcaatttttc gtatgattct ttgtgctctt ttccttcaac
    18121 ttccgctgta actactttgt tatcatagtc gattgaaaga acaggtgagt tcatgtaaac
    18181 tttagcacct ttagcttcca atttttcttt atcagaatag aacaagcctt cagcaccgtc
    18241 aatttgttca ccaatccaaa gagccattcc acatcctagg aaagagatgt tagagttttg
    18301 gtcaaataca acaatttcgt tctcatttcc aaaattatcc aacatggtat tgatacatgc
    18361 tgtaccagcg tggttagcac cgactacaac gattttactc ataaaaattt cctgccttca
    18421 ttctaaattt accttactag tataccattt accgttagcg tttacaaggg gggtactatt
    18481 atttcagcat tttttatcaa aaataattcc ccatttatcc tgttttcaag cctcatgaat
    18541 tttatgagca tatttcttga ctttttccaa attttgtttg tgaaaattct aactttatgg
    18601 tattataata acatgaaaat gaaatgtaag ggcttcaatt tagatgatta tttgatatta
    18661 caacttattt tctataagag aaaggagttg gtagcttgac tcttcgttca cattctgaac
    18721 ggctaatggg gactactatc actatttcat tagtggatga gcaagccgat atctttctcc
    18781 aaaaatcctt tgacttgctc aaagagcttg aataccgctt caacgccaat agtcaagaat
    18841 ctgagttgat ggaaatcaat tatcaagcag gaatatcccc cgtcacggtt catccagacc
    18901 tgtttgagct gatttcactt gggttagagc atagcctagc gctctctagc cacctcaata
    18961 tcagcattgg tcccttaatt caaacctggc gtatcggttt ttcagatgcc aaggtcgccc
    19021 agcctcaaga aattgaatcg gtgctgcctc taatcaatcc tcatggtatc gagttagatt
    19081 cttctacttc tactgtgttt ttaaaacaga aaggaatgaa gattgatcta ggttgtttag
    19141 ccaaaggata cagtgcggat aaggttgccc aattgcttag gaaagagggg gtgacttctg
    19201 ccttgatcaa tctgggaggg aatatcctga ccattggaaa aaatcaggca agaggggata
    19261 acccatggca aatcgggatt caagacccag ccaatcctag gggaaatcat ttaatgacca
    19321 tccctgttgt caataaatct gtcgtgactt caggcattta tgaacgtcac ctgaccgtcg
    19381 atggacaaga ttaccatcac atttttgaca gtcaaacagg atatcctgtt gaaacggaac
    19441 tagcgagtct aacaatcatc tctgataaat cagtcgatgg cgaaatctgg acgactcgac
    19501 tctttggaga aagacctgct tctatcctct ggcaagtcga aagtttggag ggcatcgaag
    19561 tcatcctcat cgataaagaa ggccacctaa gctgttcttc aggaattcct actctataga
    19621 gacaactgtt tcaatggaat tttaaaattg tattatgtaa aaagagaaag gaaatctcat
    19681 gttaaaactt attgctattg ttggaacaaa ttcaaaacgt tctacaaacc gtaaattgct
    19741 tcaatacatg caaaaacact ttactgacaa agctgaaatt gaacttgttg aaatcaaggc
    19801 cattcctgtc ttcaacaaac cagctgacaa gcaagtacct gctgaaatat tggaaattgc
    19861 tgctaaaatc gaagaggcag atggcgttat tatcggtact cctgagtatg atcactctat
    19921 tccagctgtt ttgatgagcg ctcttgcttg gttgtcttat ggtatttacc cacttttgaa
    19981 caaaccaatc atgattacag gtgcttctta cggtacgctt ggttcatctc gtgcccaatt
    20041 gcaacttcgt caaatcttga atgctcctga aatcaaggca aatgttcttc cagatgaatt
    20101 cttgctctca cactctcttc aagcatttaa cccaagtggc gacttggttg accttgatgt
    20161 tatcaagaaa ttggatgcca tctttgatga cttccgtatc ttcgtgaaga ttactgagaa
    20221 attacgtaat gcacaagaat tacttcgcaa agatgctgaa gactttgact gggaaaattt
    20281 gtaagatagg agaccaaaaa gaatgaaatt tgttggactt gttggatcaa actacgatca
    20341 atcatataac cgtaagctct tggaatttat ccgtcgctac ttcaaactca aatttgaatt
    20401 agaagttctt gaaattgacg aagttccaat gtttaaccaa gatgaaaaat gggatgaaag
    20461 tttccaatta cgttatttat ataacaaaat tactcgtgca gatggtgtta tcattgctac
    20521 tcctgagcat aaccacacta tctcagcttc actcaaatct gtacttgaat ggctttcata
    20581 cgaagttcat ccatttgaaa acaagcctgt tatgattgtg ggtgcatcat actatgacca
    20641 aggaacatca cgtgctcaag ttcaccttcg taaaatcctt gatgctccag gtgttaatgc
    20701 ctacacgctt ccaggtaacg aattccttct tggtaaagct aaggaagctt ttgataacaa
    20761 cggaaatatc accaacgaag gcactgttaa attccttgaa acttgcttag ataactttgt
    20821 taaatacgta ggagtcgttt cgaaattgaa aaaaccaaaa ccaatcgaat cagaagactt
    20881 ggattgtgga aaaccaatcg ctacaaccat cacagaagtt gaccctgacg atccagaatg
    20941 ggtagaaaaa gttgcagcaa tcactggtgc tgtttctggt gatacctatg tcaaattgga
    21001 ccacggtatc ctaacagtta accaaattga tatgttcttg aaagctatgc catttgaatt
    21061 gacatacgct gacgacaaca accaattcct ctactacaac aacgctcacc aagatccaga
    21121 cactatgttc gctaaacgtg taccacctca atcaggtagc cgtatgtcga ctgttcatgg
    21181 ttctcttcca ccagcacgta tgaagaacgt agagtgggtt atcggaacac ttcgcaacgg
    21241 aaaccaagaa tacgtccgta cgatcgttcc aggttctcct gcaggtgtta tcaacactca
    21301 caactaccaa gcaatgtact atcctgatgg atcatacgct ggtatcaatg aaatcgtctt
    21361 taacttccaa ccatggcttg actggtacct aaaagagact ggtcaacgtt tggtgggtgg
    21421 tagcggacca tttgctcctg ctgccggagg tcatggagac gccgatgcta cttctggcgc
    21481 ttctgattca ggggatgctg gaggccacgg tggtgacgca gacgctacag ccggtgcaag
    21541 ttactaatga tagtttttag gaaccattat ggttcctttt ttagcgaaaa aagactagca
    21601 aacacaaact gctagtctct ttgttatcgt ttcatctatc tttgttaaaa gaaagagtac
    21661 atattatgaa tttgggtcat caagactacg gccatgtaat cctttttcac gttgtacttg
    21721 gcgtagtttt tctggtgtaa catcgtttcc ttcttcgtcc acaattttga ttccttcaat
    21781 gtggtgacga acagcgcggc gataaccttc gatgtactcc tcacgtagtt tggcttgttc
    21841 cactttttct tctggtgtta agccttctgt ttttttcttt ttagcaagct cattgatacg
    21901 agcaattttt ttcggatcca tttcttctcc tttgtaataa gataaagtcc gtttatcaaa
    21961 ttttatttag tgttaatttg gttaaaacaa gcaaacttag ctgctttctt ggttagttgt
    22021 gacaagattg ccaaacgatt ttgacggaca gcctgatctt cagccattac catagtattt
    22081 tcaaagaaag catcaatgac tgggctaagc gcaaagagtt gtttcaattg ctgacttgca
    22141 ggtcctgata aaacgagtgt ttctactgct tctgccaaag ctttttcttg gtcattctca
    22201 aatagtgctg aatcaactgt agcaacccct tctgccttct cagccaggtt aaaagcacga
    22261 gaaagtgatt caacagatgg tttaaaatct tcttccttgc ttacttctac gagagcactt
    22321 gctgcttcca acatatctgc cacaacaaag tttgaacctg caagaactgc ttccttgata
    22381 tcttttggag tagagcccat catcttatca acacgagcct tgataaagtc cataacctct
    22441 gctttatttt cataagtcaa actgtcaaat ttcaatgcat aaaggctatc aatcagctca
    22501 tccatagcaa tgtgccaacc aaaggcaccc aagatacgaa ccacaccttg agttgcacga
    22561 cgaagggcat aagggtcatt agaacctgat ggaatcaatc ctactgagaa gaaactcaaa
    22621 atcgtatcca atttgtctgc aatggctaga actgcgccga ccttgctctc tggaagttct
    22681 ccttcagctg atgtaggcat gtagtgttca cgaatagcag ctgccaccgc tggagtttca
    22741 ccagcaagaa gggtgtattt ttcacccata attccttgga gttcgtcaaa ttcaccaacc
    22801 atacctgtca acaagtcaaa cttgtaaatg gctgctgcac gagcaaggtc aactgtttca
    22861 tccactgaca aaccagcttt ttctgccaaa agtacagtga tttgacccgt acgaatcatg
    22921 tgttcacgaa gggaaccaat cttctcatgg aaggtgacat tgtttaattt ttcaacaaga
    22981 tctgaaatca ccaatttttg gtcttcacgc cagaagaatt ctccgtcttc caagcgggct
    23041 actaagactt tttcatttcc tttgatgaca tttttcaaac gctctgcgtt tccgttacga
    23101 acagaaatga agtttggcaa gagttttcca tcttgatcac gaacaacaaa gtaacgctgg
    23161 tgttccttca tagaagtcac caagacttct tctggaactt caaggtattt agcatcaaaa
    23221 cttcccatga aggcagttgg gtattcaact aaattcaaga cttcattcag caaatccgca
    23281 tcaatttcga tacgtacacc atgttttgcc tcaattttct tgatttggtc aacaatcatt
    23341 tgctcacgtt cacatggatc tgcgattaca aactgcttac gaaggtcttc ttcatagctc
    23401 aatgctgact gaattttggt ttctttcccc aagaaacgat ggccacggct cacacgactt
    23461 cccttgatat caaggaaatc caagtcaaac tcttgctcat ccaagagaac agttaaagtg
    23521 tgaacagggc ggatgtattc aaagctattt ccagcccagt gcatgctgac agggaaagtc
    23581 aatgacttca agacatctac aacaccagga acaatggctt caactgattg gccaacttct
    23641 tccttagtga catagacata ttcttcaccc ttaatttcac ggaattcgat atcttcaaca
    23701 gtcaaacctt tcccacggac aaatccttga gctgctttgg tgaagtttcc atcactatcc
    23761 aaggcaattt tctttgctgg tcccttgaaa tcttctgtca aatcagactg tttgtctgat
    23821 aaaccagtca cacgaacagc caaacgacgt ggtgttgaga aggtttgaat ggcttcaaaa
    23881 gacaggcggt tttccttgag gaaggctgcc attttttcgc ctagttgttt ttcacttggt
    23941 gtgacaacat aggctggtaa ttcttcaaga ccgagttcta ctaataagtt ttttgtcatg
    24001 ttttttctcc gaaaagtatc tttttatttt ccagtttgcc ttatgtgatt ggcacgcaag
    24061 caaccaactt cgttgtttca ttgctaaaat tggagccaaa agtctccaat tttgcctagt
    24121 atccttagtt tcactaagga taccttcatc actgcggcaa ctggctcagg gctcactctg
    24181 ttcgcccatt ttcgtaattt gtcactctct ttattctgcg tcttctgcta ggagtttagc
    24241 tcttgtttct tcatccaaaa gtgggtagcc taggcgtttg cgttctgcga caaaggtttt
    24301 ggctacgaca cgggccaagt tacggatacg agcgatatag cctgcacgct ctgttacaga
    24361 tacggcacca cgcgcgtcaa gcagattaaa ggtatgtgaa catttgagaa catagtcata
    24421 ggcagggtgt accaagcctt cttctaatgc acgaccagct tctttttcaa acttatcaaa
    24481 attttcaagc aacatttctt ggtccgaaat ttcaaatgaa tattttgagt gctcatactc
    24541 aggctggata aagatttctc cgtattttac accatcagcc cactcgatat catagacaga
    24601 gtctacttct tgaatgtaag aagccaagcg ctccaaacca taggtaactt ccgcagtcac
    24661 agggctagtt gccaatccac cgacttgttg gaaataagtg aactgagtga tttccattcc
    24721 gtcaagccaa acttcccaac caagaccagc tgaaccagtt gatgggtttt cccagttgtc
    24781 ctcaacaaaa cgaatatcgt gctccaaagg attgattccc aatttttcca aagactcaag
    24841 gtaaagttct tggatatttg atggagaagg cttcatgacc acctggaatt ggtggtgttg
    24901 gtagagacgg ttagggtttt ccccataacg accgtcagca ggacgacgtg atggctctac
    24961 ataagctgca ttccatggct caggtccgat agcacgaagg aaagtgtaag gactcattgt
    25021 tcccgcacct ttttcattgt cataagcctg cataagcata caaccttggt cattccaaaa
    25081 ttgttgcaaa gtaaggatga tttcctggaa agtcaatttc ttagacattg tttactcctt
    25141 aataaaaaat gatttcttgc gacatgagtc tgcctcgtcg atcatacgag gcaagacgag
    25201 ttagtactgc gtctagctca ggcactctag tgctgagcgt ggggcagtcc gactgtaagg
    25261 aggtttctgc cactgatgca gtgaaatgtt aatttcttag acattgttta ctccttaata
    25321 aaaaatgatt tcttgcgaca cgagtctgcc tcgtcgatta tacgaggcaa gacgagttag
    25381 tactgcgtct agctcagcac cctagtgctg agcgtggggc agtccgactg taaggaggtc
    25441 tctgccactg atgcagtgaa atgttaattt cttagacatt gtttactcct ttagttttta
    25501 tatttctttt tcaattaatt tctgctgaac aaaccaattt aacaagggaa gagttttttc
    25561 tgtatttgcc tatagatgat aagaatctaa gaccgcttgt acactaccta aattttctac
    25621 caacttatct actgtcaaaa agctgcgcca gtattcataa aaaatacttg catagttctt
    25681 atcataagtc gaagaaccaa aatcattcaa tgaatgccaa ccatacttct tctgaaaaag
    25741 ttctacgaga gattgattac aaattttttc cgcttgaaat tcctcttctg taaagaaata
    25801 cttgcgacta atatattcaa ccatcccctc ttcgaaccag atataagagt cataaccatc
    25861 aaaatcatct aaaaaatgtc cgaccaatga gctaactcat gtcctacaat ctgtaagagg
    25921 aaattttcag ataaagaatg atagtgactt tctattgctt gagtttgctc agaacactcg
    25981 tagtccatca actgatacaa atacaactct ttccaaacag ctaaatcagg cgtcataacc
    26041 attcgtctat tatttgtata ggctggaaca gcactttccc taataatctg tgtagcagca
    26101 ttaaaatctg accaaataac ggcttgcggt aaatcacaga ctgcaagttc attctttaaa
    26161 aaagattgat aatctttcaa cttttcagta tttcttacta caaaatcacg aaatgtagct
    26221 agttgactat cgtcttttac aagataaagg ttgtccacgg ctctcctttc tttcacacaa
    26281 tagactgaat aagcaaaaga accacatctg acaatcctag gttgatttaa cctaggggcg
    26341 tcagaaacgc ggttccaccc taatttatta cttcattgtt tttgaaaata ctacagaaag
    26401 cgccagctca ttattttgcc ctacttggct cccactgtcc caagctcgct tgaagaacgc
    26461 cataagactt tctttcctgc tgactattat atcaaaaatt agaataatgt acaattcttt
    26521 ttatgatttt ctagaaatcc acatcatcaa ctcgtggagc ccccgactga acagcagtcg
    26581 tttttaaggt ttctctctcc tcatgactca gttcaattcc aaaacaatca agattagctt
    26641 gaatacgaga ggttgtgaca gattttggaa gtggtaaaaa tccttctgcc aagctccagg
    26701 ccaaggctat ctgagcaacc gattttccgt gatttgctgc gatttcttgg acttgcttgc
    26761 tatcaaacag ttctccttgt ccaaaaggcc cccaagcttc caataaaatt cccttttcac
    26821 gacagtaagc tacgacttcc tcttgataca cacctggcgc caagcgaact tgattgaccg
    26881 caggaacgat agttgcagtt tcaagcaagg catccaaatg atggggaaga aaattgctaa
    26941 cgccgatagc acggattttc ccttcttgat agaggtcttc catcgctctc caaacttccg
    27001 cattgcgagt tttccatgcg tcattttctc tgagcggttt tgggttcggc caatgaatca
    27061 aatacaaatc caaataatcc aagcccagtt tttctataga tttttccaaa gcttgacgag
    27121 tttgctcata ggtttgctga ctattccaaa gcttggtagt tacgaacatt tcttcacgtg
    27181 gaactccgct atctttgatt gcttgaccaa cactttcttc attctgataa atcgccgccg
    27241 tatcaatatg acgataacca gccttcaagg attctaacac tgcacgatag gcttcttctc
    27301 catccttagc cttaaaagtt ccaaatccca atactggaat ttctactcca ttatttaatt
    27361 gataggtatt catatgcttc tccttctata tttattcatc aattatagca taaaacattt
    27421 tttaagtagt actatcttct gtaaaaaata agtctgtcaa atatctaaca gacctatttt
    27481 ttcttgctta ttcatcacga ctatagtaca gctcatgagc ttttaggcga gtattgagca
    27541 tctctggtat ggtcacaaag gtataacctt gatttttcaa atactcaatg acccttggca
    27601 aggcattgac tgtcggactg tgaatatcat gcatcaaaac gatagagccg ttagctactt
    27661 gatgctgaat ttctgtcaaa atagatgctt catttttact cttccagtcc agactatcca
    27721 catcccacat gataaagctc aaatccaagc tattgcgaat atcatctgta atagcaccat
    27781 aaggtggacg catgagttta gaactagaac ccagcacttt agttagcaca tcctcagtat
    27841 cagtaatctg ctttttagct tcatcaagag agagttgcga gagaatcgga tggctccaac
    27901 tatggtttcc aacaacatga ccttcagatt ttatcctctt caccaagtcc tcattcccag
    27961 aaacattttt cccaagcaca aagaaagtcg ctttaatatc atatttagct agggtctcta
    28021 atacctgcgg ggtcgttgct ggatttggac catcatcgaa ggttagagcg acaacttttt
    28081 gatatttctt atcaaagtaa gattggtaca aggccgcatc tttttcgagt aagtacgaag
    28141 attggataac atcaaagaaa gcagatactg gcaaggctat ctcttctaaa ttttcaacca
    28201 caggacttgg ataaaggata atctgactat ccttgtaatc aaaattccat gcagacaagt
    28261 cttggtcaga gaagcttttt acaatctgct cactctggtc ttgctctatt tttttatcct
    28321 ctatgaagga ggtcaactct tttatcagct gttccttagc cttactagca tctgaaaaca
    28381 gttggtcaag tgtaaaaggt tgcccatctt ctgttaagtg aacctttcct agactggtct
    28441 tttcagtctc ttcaacctta aaagcaacta aatcatagat ttgtttggtc acattacgat
    28501 taacgactcc ctttaaacct gactcttctt gctctgtata gtagaaaaca agattgtcct
    28561 tactttctag cttgtcctta acatcttgat ttatcaactc cctaacagag gaaatcactt
    28621 tctccccttg gagaggataa taggcaatca cttcggcttg cccctgacga aaatgctcct
    28681 tctgatttcc ctcactcaat tgatcatctt tctctttttt gagcgattca atcttttgct
    28741 caaaactttt ttgctggtag atcttaaaag caataaatcc gcctaatagg caaatagaaa
    28801 ttgctaaaat accaatcaaa gccaataata catgtctcgt tttcccgtgt ctgccacgtc
    28861 ccagtctact tttatccata tttatatcat aacaaatttg agcaataatt tcaatgatac
    28921 aatttggcaa tccacaattc ctctagcaag ccaaagcatc ttacatcaac tccaacatcc
    28981 gtatctgtag agattatgta gttttctatc tatggaattt ctagtagatt gaagctagaa
    29041 tagtagacct atacttctaa aacattgtta gaaatcgatt ttactgtcct gatcgatttg
    29101 tcctattctc atttcatttt actataaaac tatgaaaagc taggtcatct gagaagtaat
    29161 tactatcgac aattgcgata gcgtatactg gtgcgatttg tttgtagctc tggtgggtat
    29221 caccttaacg ttggcgaatt ttttctaggt tttgattaac ctgactgcac agataagccc
    29281 ataggcgatt gatgaaaaaa ttctgatgat gaatctgaat ttcgatgata acttggattc
    29341 cattatctaa ctccgccaag acgtctatac tagtatagaa atcctgcact gagtagggca
    29401 tggaaggcaa gacgtgaatg ttacttccct ccaaaatcgt cacatttttt gctggcaagt
    29461 ccagcatatc gcggataaat tgacaaatca ttagttgggc tgatacccgg atgtctgaga
    29521 atcctccttt tcctccttct attatagcac atttttaaat ctaggtttgc tagattctat
    29581 gcttctatct atttattcgg aaaagaaagc ttttcttcag aagaaagcta atagcttatt
    29641 tgaaattttc tcctctctgt tggatagtcc cgtctatcta tgttataatg aaagaaggat
    29701 ttaaaatcgg aaaaggagta tttatgctaa aattaggtgt catcggaact ggcgctatca
    29761 gccatcattt catagaagca gcccatacca gtggagaata ccaactggtc gcaatctatt
    29821 ctagaaaact agaaactgca gcaacctttg cttctcgcta tcagaatatc caactattcg
    29881 atcaattaga agtcttcttc aaatcttctt ttgatttagt ctatatcgcc agtccaaact
    29941 ccttacattt tgctcaggca aaagctgcct tgtctgctgg taaacatgtt attctcgaaa
    30001 agccagctgt cactcaacca caagaatggt ttgatttgat tcaaacagct gaaaaaaata
    30061 actgttttat ctttgaagca gctcgtaatt accacgagaa agcctttact actatcaaaa
    30121 actttttagc agataagcaa gtgttaggag cagatttcaa ctatgccaaa tactcttcca
    30181 agatgcctga cttgttgact ggacaaacac caaatgtctt ttcaaaccgt tttgctggtg
    30241 gagccctcat ggatttgggg atttatcccc tctatgctgc tgttcgtctt tttggaaaag
    30301 ccaatgacgc gacttaccat gctcaacagc ttgacaatag cattgaccta aatggagatg
    30361 gtatcctctt ctacccagac tatcaagttc acatcaaggc tggaaaaaac atcacttcca
    30421 atcttccttg cgagatttat acaacagatg gaaccttgac tctcaacacg attgagcata
    30481 ttcgctcagc tatttttacc gaccaccaag gaaatcaagt ccagcttcct atccaacagg
    30541 ctcctcatac gatgactgag gaagtcgctg catttgcaca catgatccag caaccagatc
    30601 tgaatctcta ccaaacttgg ctgtatgatg caggctctgt tcatgagcta ctatatacca
    30661 tgcgccagac tgctggcatt agatttgagg cagaaaaatg aaaaccaaac taccgactga
    30721 atggcaagaa ctgagtgacc aactcggttt ccaagaattc acccccattc aaactcaact
    30781 atttgagccc cttcttgctg gagaaaacct cctaggagtg agccaaaccg gaactggtaa
    30841 gaccctagcg tatctcctac caagtcttct cagactacaa aagaaaaaag cccaacaact
    30901 cttgattcta gcaccaaaca cagaacttgc tggacagatt tttgatgtat gtaaaacgtg
    30961 ggcagaagct atcggattga ctgctcaact cttcctttca ggttcaagtc agaaacgaca
    31021 aattgaacgc cttaaaaagg ggccagaaat tctgattgga actcctggcc gtatctttga
    31081 actcattaaa cttaaaaaaa tcaagatgat gaatgtggaa accatcatcc tggatgaatt
    31141 tgaccaatta ctagatgatt ctcagattca ctttgtagag aaaatcactc actacgcacc
    31201 tcgtgaccac caactggtct acatgagtgc cacgaccaag tttgaccaag aaaagattgt
    31261 gccgaataca cgcactattg atctctctga tcaaaaattg gacaatatcc agcatttcta
    31321 catgcaggta gaccaacgtc atcgagtgga tatgctacga aaactggctc atgtagagga
    31381 tttccgtggt ctagtcttct tcaatagcct gtcagacctt gggaatgccg aggaaaaact
    31441 acagtatcgt gatatattgg ctgtttccct cgctagtgat gttaatgtta aatttagaaa
    31501 agtcatctta gaaaagttta aagataacca actaaccctg cttcttgcaa ctgacctttt
    31561 ggctcgtgga attgatatcg atagcctaga atgtgtcgta aactttgata ttcctagaga
    31621 tagcgaaacc tacatacacc gtgctggccg tactggtcgt atgggcaagg aaggttatgt
    31681 tatcactctc gtcactcatc cagaagaaat caaaaaactt aaaaagtttg caagtattcg
    31741 agaaattgtc ctaaaaaacc aagaactcta tatcaaataa agccccattg ctctatacac
    31801 atccagtgta tggagctttt ttataagcaa ttcaatttta tactcatctg ctttcaaaaa
    31861 gcattctagt ccatctccga ttaacgatgg actttatcac ctccttctcc aatccttgta
    31921 tgacatcttg gagttgattc atgacatctt ccaaaattcg aaaggcttta ttcttaaatc
    31981 cacgtttacg aatctctttc cacacttgtt caatggggtt catctctggt gtgtatggag
    32041 gaataaatgc aaaaccaata ttagtcggaa tctttaaggt acttgattta tgccatatag
    32101 cattgtccat aacgagtaaa agataatcat ctggataagc ttgtgaaagc tcttctaaaa
    32161 aggcgttcat ccactcagta ttacatctac cagctattaa gaaaaatgat tcgcctgtat
    32221 aggcatcaac agctccataa caatagcgaa attctcgtat atagtgacta tggatatgtg
    32281 gacctactcc tattggagcc caacaagatc ccagtttact gattctaccg aaaccagctt
    32341 cagcttggta catcaagcaa accttacgaa aacgtctact atatttaaaa cgctttcttg
    32401 ccttcttgga ttgagatttt atttttagac gcaacaatgg tttgagcgtc tgctttctta
    32461 ggatgttctg gacgtggcgt aatatttcgc caaccatggc gcttcaacag ttgatagaag
    32521 gcatcacgtg tgtaggaacg acctaactcc tttttataag cctgaaataa ggcatcaatt
    32581 gtaacaaatt ctcctgcctc tgtagccttc aaatggcggg caagaaaggc tttctcttcc
    32641 tcaactgtca tatatgcatg gttacgacca ccacgtgttt cttgaaggag agagtcgagt
    32701 ccgaactcct catatttttt tacatttcgc caaatcgttg tttgattaca gtctaaaaga
    32761 tctataatct ctttataaga tttgcccatc agacgaaata gaacgatttg aaggcgttta
    32821 tgatatttag ctgtacgaaa gtcttttaaa agtgttttga tggtttggat ttcttcttta
    32881 gttgatttca tattactatt gtataatgct ttttgatttg agtctagtat tatactcttc
    32941 gaaaatctct tcaaaccgcg tcagcttcgc cttgtcgtat agatgttact gacttcgtca
    33001 gttctatcta caacctaaaa acagtgtttt gagcagtctg cagctaactt tctataacaa
    33061 aatgaaataa gaacaagaca aatcaatcaa gacaatcaaa tcgatttcta
//
LOCUS       DAVGJL010000008        33010 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.008, whole genome shotgun sequence.
ACCESSION   DAVGJL010000008 DAVGJL010000000
VERSION     DAVGJL010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33010
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.008"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            98..370
                     /gene="rpsP"
                     /locus_tag="VVE42_000318"
     CDS             98..370
                     /gene="rpsP"
                     /locus_tag="VVE42_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEU3072583.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLAWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
     gene            390..629
                     /locus_tag="VVE42_000319"
     CDS             390..629
                     /locus_tag="VVE42_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEU3072584.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
     gene            859..1102
                     /locus_tag="VVE42_000320"
                     /pseudo
     CDS             859..1102
                     /locus_tag="VVE42_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779614.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
     gene            1206..1994
                     /locus_tag="VVE42_000321"
     CDS             1206..1994
                     /locus_tag="VVE42_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU3072585.1"
                     /translation="MKRFEVSTEIGSLSVAYQKQKKVLVCLNGAGLLPSYENFSLILE
                     KPPPTIGYLTIDFPNTGRSPIHDQAGKNLDNLADVVYEVLEELGISEYILCAHSWSGI
                     LACKLLEKPIKCQTLVAIEPTTKKVMFADFSENPYPEMEEQMRLIDECGPELYFKNLT
                     QATFSPETNKKIWELMQEKGLELENQDPEFQISGEITEEDFEDVSIEAHIPVFVFCQP
                     YREKEYRESEYWTSNTKLILGGNHHYLQWSESEKIAAIIRELSE"
     gene            2025..2543
                     /gene="rimM"
                     /locus_tag="VVE42_000322"
     CDS             2025..2543
                     /gene="rimM"
                     /locus_tag="VVE42_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEU3072586.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELALFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKGYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPNKRV
                     DVEILEGLDDED"
     gene            2533..3252
                     /gene="trmD"
                     /locus_tag="VVE42_000323"
     CDS             2533..3252
                     /gene="trmD"
                     /locus_tag="VVE42_000323"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEU3072587.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDEPYGGGQGMLLRAQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            3264..3602
                     /locus_tag="VVE42_000324"
     CDS             3264..3602
                     /locus_tag="VVE42_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3072588.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(3633..3953)
                     /locus_tag="VVE42_000325"
     CDS             complement(3633..3953)
                     /locus_tag="VVE42_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3072589.1"
                     /translation="MRHKFQQVLDKIHDFLNGHDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFAKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            4051..4266
                     /locus_tag="VVE42_000326"
     CDS             4051..4266
                     /locus_tag="VVE42_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEU3072590.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFKGN"
     gene            complement(4335..4421)
                     /locus_tag="VVE42_000327"
                     /pseudo
     CDS             complement(4335..4421)
                     /locus_tag="VVE42_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(4472..5008)
                     /locus_tag="VVE42_000328"
     CDS             complement(4472..5008)
                     /locus_tag="VVE42_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEU3072591.1"
                     /translation="MKKAHVYAIPAIGAALIAVLAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLHFLAGMAFEKALVVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQTYFTN"
     gene            complement(5205..6551)
                     /gene="gor"
                     /locus_tag="VVE42_000329"
     CDS             complement(5205..6551)
                     /gene="gor"
                     /locus_tag="VVE42_000329"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEU3072592.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGAKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFNFATLRRNREAYIDRARSSYDGSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESIAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEME
                     RTNLPLHTHKVPVKLEKTTDGITIHFEDGTSHTASQVIWATGRRPNVKGLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGIYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFASMYSACTRNRQESRFKLI
                     TAGSEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTASEEFVTMR"
     gene            6837..8036
                     /locus_tag="VVE42_000330"
     CDS             6837..8036
                     /locus_tag="VVE42_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU3072593.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPSQTAVKDE
                     PTHLVVAKEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKVSEGQALVK
                     YSSSEAQAAYDSASRAVAKADRHINELNQARNEAASAQAPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPAAGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILVPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KADEATN"
     gene            8020..8721
                     /locus_tag="VVE42_000331"
     CDS             8020..8721
                     /locus_tag="VVE42_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3072594.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            8723..9982
                     /locus_tag="VVE42_000332"
     CDS             8723..9982
                     /locus_tag="VVE42_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU3072595.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSAFTVSGKEEEVPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKRSKIYGFGGLP
                     ITTNISLAANFNVDEIASIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESL
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            10106..12103
                     /gene="metG"
                     /locus_tag="VVE42_000333"
     CDS             10106..12103
                     /gene="metG"
                     /locus_tag="VVE42_000333"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEU3072596.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYIGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLAKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIVAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTLLTVDPAVPNGSVIG"
     gene            12147..12227
                     /locus_tag="VVE42_000334"
                     /pseudo
     CDS             12147..12227
                     /locus_tag="VVE42_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            12659..12979
                     /locus_tag="VVE42_000335"
     CDS             12659..12979
                     /locus_tag="VVE42_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU3072597.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMRPSPDGPDRKYFSLTKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
     gene            12983..13798
                     /locus_tag="VVE42_000336"
     CDS             12983..13798
                     /locus_tag="VVE42_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3072598.1"
                     /translation="MKKMKYYEETSALLHEFSEENQKYFEELWESFNLAGFLYDEDYL
                     REQIYLMMLDFSEAERDGMSAEDYLGKNPKKIMKEILKGAPRSSIKESLLTPILVLAV
                     LRYYQLLSDFSKGPLLTVNLLTFLGQLLIFLIGFGLVATILRRSLVQDSPKMKIGTYI
                     VVGTIVLLVVLGYVGMASFIQEGAFYIPAPWDSLSVFTISLVIGIWNWKEAVFRPFVS
                     MIIAHLVVGSLLRYYEWMGISNVFLTKVIPLAVLFIGIFLLFRGFKKIKWSEV"
     gene            13873..14799
                     /locus_tag="VVE42_000337"
     CDS             13873..14799
                     /locus_tag="VVE42_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HEU3072599.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVEELVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDALMESDQVAEAFDLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSAAFTPGFESAFHVNMEDSQAAMRDGSIFEYCQLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSQAANFSLTRKEWYEIYLAAGNNLP"
     gene            14899..15135
                     /locus_tag="VVE42_000338"
     CDS             14899..15135
                     /locus_tag="VVE42_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEU3072600.1"
                     /translation="MTFEEILPGLKAKKKYVRTGWGGAENYVQLFDTIEQNGLALEVT
                     PYFLINVSGEGEGFSMWSPTACDVLATDWVEVHD"
     gene            15128..15826
                     /locus_tag="VVE42_000339"
     CDS             15128..15826
                     /locus_tag="VVE42_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEU3072601.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKPLLEGDF
                     HFLQRDLTLDLEPIFDWCPQVDVLCNTAGVLDDYKPLLEQTAQDIQEIFEINYIIPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEAGI
                     QVFGIAPGAVKTAMTAADFETGGLADWVASETPIKRWIEPEEVAEVSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            15837..16337
                     /locus_tag="VVE42_000340"
     CDS             15837..16337
                     /locus_tag="VVE42_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU3072602.1"
                     /translation="MEIKNHFGVYCVCFENGKLLCIEKTRGPYQHRYDLPGGSQQLGE
                     GLTETLTREVMEETGFTVRSYSNPRIYDVFVREELKNFMVHHVIALYDVEMNESAPQV
                     TISEAVSDGANDSLGYIWMDIQEITEENASPLVLKVKSELLGLPELDKTSYMNWKVND
                     EKSTCP"
     gene            16315..17184
                     /locus_tag="VVE42_000341"
     CDS             16315..17184
                     /locus_tag="VVE42_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme, TIM barrel domain protein"
                     /protein_id="HEU3072603.1"
                     /translation="MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNIILAS
                     CQQFVKDYLDTICSFYLDKEVWYRFSELTNTEANCLAGTREFFDEGHPLFGYRGTRRL
                     LACLDEFQAESHVVTEVYQTNPNLSLIFPFVNDADQLKQAITVLRQYGFTGKVGTMIE
                     LPSAYFDLSSILETGISKIVVGMNDLTSFVFATMRNSQWHDMESPIMLDMLRDMQDKA
                     RKNKINFAVAGYLNTSFIQKMNQLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKK
                     LQRSTHDTARNVE"
     gene            17159..17599
                     /locus_tag="VVE42_000342"
     CDS             17159..17599
                     /locus_tag="VVE42_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEU3072604.1"
                     /translation="MIPQEMWNKYKQINPLIGDEIDAWAFGVEPDLLADLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDVILDSQNQAVCVVEITKVSVELFNQVSAQHAFKEGE
                     GDKSLAYWRQVHEDFFTDCLGEVGLTFTPESKVVLEEFRKVYPL"
     gene            17722..20268
                     /locus_tag="VVE42_000343"
     CDS             17722..20268
                     /locus_tag="VVE42_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEU3072605.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLSLRFDQAEGELALSNMP
                     EIDVENRKETGIWKFETTPRMSSYLLAFVAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVRSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENDVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLEAVLAELETLDNTSKLQIVQERRLLAEAGHISYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEASQVASQIFATHKENLAGLPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLAAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
     gene            20377..21051
                     /gene="ciaR"
                     /locus_tag="VVE42_000344"
     CDS             20377..21051
                     /gene="ciaR"
                     /locus_tag="VVE42_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HEU3072606.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
     gene            21041..22375
                     /gene="ciaH"
                     /locus_tag="VVE42_000345"
     CDS             21041..22375
                     /gene="ciaH"
                     /locus_tag="VVE42_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HEU3072607.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(22409..22696)
                     /locus_tag="VVE42_000346"
     CDS             complement(22409..22696)
                     /locus_tag="VVE42_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEU3072608.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFAAAIGFSLLVLKVQRSIIKRKRRR"
     gene            22836..23765
                     /locus_tag="VVE42_000347"
     CDS             22836..23765
                     /locus_tag="VVE42_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEU3072609.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELIVAVNALMHQDMMDRIKPFLDFLEEIKTDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARSLIQEGNFSH
                     AQAFLLDEEVRKLHPKNRFLDTGFYDYDPDMVR"
     gene            23965..26415
                     /locus_tag="VVE42_000348"
     CDS             23965..26415
                     /locus_tag="VVE42_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEU3072610.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDHYTTD
                     VNPHEPLDAHIKELTGLTDQRLAQAPDFSQVARKIFDLVEDGIFVAHNVQFDANLLAE
                     NLFFEGYELRNPRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLRKKMTQLPKGLLERLLEMADALLYESYLVIEETYRNQSILSSPDLVQVQGLYF
                     KKTEASLEPRKLSQDFSKNISLLNLEVREEQESFAKEVGLLLKDEPVSLIQAPTGIGK
                     TYGYLLPALSQSKERQIVLSVPTKILQNQIMEEEGKRLKEVFHTDIHSLKGPQNYLKL
                     DAFYHSLQENDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SQSLFVTEDFWKRSQERAETCKLLVTNHAYLVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRILESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEDFDELVRYFTAEGDYWLEVTETSQKKIQISSTKSGRTLLSSL
                     LPESCQVLGVSATLEISQRVSLADLLGYPEAKFVKIESRGKQEQEVVMVKDFPLVTEN
                     SLEVYAREVAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVSHLAQYKNGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPSVIQVVPRLPFQNPQEPLTKKINQELNQEGKN
                     AFYDYQLPMAIIRLKQALGRSMRREYQRSLTLILDRRIVGKRYGKQIVASLAEEATVK
                     TISRSEVDEAIDRFFNEL"
     gene            26452..27675
                     /locus_tag="VVE42_000349"
     CDS             26452..27675
                     /locus_tag="VVE42_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEU3072611.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPRLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVPLDFLLIFWMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            27686..28240
                     /locus_tag="VVE42_000350"
     CDS             27686..28240
                     /locus_tag="VVE42_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEU3072612.1"
                     /translation="MVKVAVMLAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGNLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDGVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYRDVFGKNQ"
     gene            28325..28888
                     /locus_tag="VVE42_000351"
     CDS             28325..28888
                     /locus_tag="VVE42_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU3072613.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQA
                     LKVSTPFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEVISNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            28903..30849
                     /gene="gyrB"
                     /locus_tag="VVE42_000352"
     CDS             28903..30849
                     /gene="gyrB"
                     /locus_tag="VVE42_000352"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEU3072614.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
                     LEIVGDTDKTGTTVHFTPDPKIFTETTIFDFDKLNKRIQELAFLNRGLQISITDKREG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            30931..32658
                     /gene="ezrA"
                     /locus_tag="VVE42_000353"
     CDS             30931..32658
                     /gene="ezrA"
                     /locus_tag="VVE42_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEU3072615.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEERKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAEGYNHSF
                     RFLKASHQIDQIESQITLIEEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLEAGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEVTSNQEEPTQAYSVLEENLEDL
                     QTQLKDIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
BASE COUNT        10117 a         5903 c         7241 g         9749 t
ORIGIN      
        1 ttcttgacac ctgccagaaa tctgctataa tagaacatgt gctaaatagc tcagctattt
       61 caccgaaata aaaaattaaa gaaatgagac tttaaaaatg gcagttaaaa tccgtttgac
      121 tcgtatgggt tctaagaaaa aacctttcta ccgtatcaac gtagcagatt cacgttcacc
      181 acgtgacgga cgtttcatcg aaacagttgg aacttacaac ccacttgttg ctgaaaacca
      241 agtaactttg aaagaagacc gcgttcttgc atggttggct aatggagctc aaccttcaga
      301 cacagtacgt aacatccttt caaaagaagg cgtattgaaa aaattccacg attctaaatt
      361 ctcaaaataa gtttaaagta ggttgacaga tggatacgat tgaaaatctc attattgcga
      421 ttgtgaaacc cttaatttca caaccagatg ccttaactat caagattgaa gatacaccag
      481 aatttttgga atatcatttg aatcttgatc aaagcgatgt gggtcgtgta atcggtcgta
      541 agggtcgcac tatttctgcg ataagaacga ttgtctactc tgtcccaact gaatacaaaa
      601 aagtaagaat cgttattgac gaaaaataag aagggcggga cggatgtctc gcttttttgt
      661 gtggaataga agatatagta aaatgaaata ataacaggac aaatcgatca ggacagtcaa
      721 atcgatttct aacaatgttt tagaagtaaa ggtgtactat tctagtttca atatactata
      781 aaagattgaa cgtttaggca attccaagac tacttactcg aacattaaca gcagtctcgg
      841 actgatgaag gtctctttat gaagttagtg gaggaagtcg gagaagtagc cgaggttttg
      901 aataggcgct ctggtcgaaa agaagggata ggattcgaac gaggaacttg ccagagaact
      961 ggctgatatc atttactaca ccgtcgcaat cgtagctatt aatcatattg accttaccaa
     1021 gactgtcttt gagaaagaca aaaccgcagc cgttaattac caacataaac atgatttgga
     1081 aggatttttg aagggaaagt agagatgttg tccccttcct ccaacttgag aaaatccttg
     1141 gaacgtgata aaataaagga taaggatttt taataattca ttatatagaa tgagtggatt
     1201 tctttatgaa gaggtttgaa gtatctacag aaattggtag tctctctgtt gcttatcaaa
     1261 agcaaaagaa agtgctagtt tgtctaaacg gagcaggttt gctaccaagt tatgaaaatt
     1321 tttcacttat acttgaaaaa cctcctccta caattggtta tttgacaatt gattttccga
     1381 acacaggtag gagtccgatt catgaccaag ctggaaaaaa tctagataat cttgcagatg
     1441 tggtttatga agtacttgaa gaattaggga tttctgaata tatactttgt gcacatagtt
     1501 ggagtggaat tttagcttgc aaattactcg agaaaccaat taagtgtcag actttagtag
     1561 caattgaacc gacaactaaa aaagtcatgt ttgctgattt ttcagaaaat ccttatccag
     1621 aaatggaaga gcagatgagg ctgattgacg agtgtggtcc tgaactttat tttaagaact
     1681 taactcaagc aacatttagt cctgaaacga ataaaaaaat ctgggaattg atgcaagaaa
     1741 aaggcttaga gttggaaaat caagatccag aatttcagat atctggagag attactgagg
     1801 aagattttga ggatgtatct atagaagctc atattcctgt atttgttttt tgtcagcctt
     1861 atagagaaaa agagtacaga gaatcagaat attggacttc caatactaaa ctcattttag
     1921 gagggaatca ccattattta caatggtcag aatcggaaaa aattgcggct attattcgag
     1981 aattgtcaga ataagatgga aaaaaggaga ttacaggaga caagatgaac tactttaatg
     2041 ttgggaaaat cgttaatacg cagggattac agggtgagat gcgagtcttg tctgtgacgg
     2101 attttgcaga agaacggttt aaaaaaggag ctgagctggc tttgtttgat gaaaaagatc
     2161 agtttgtcca aacagtgacc atcgctagcc accgtaaaca gaagaacttt gacattatta
     2221 aattcaaaga tatgtaccat atcaatacta tcgaaaagta caagggatat agtctcaagg
     2281 tcgctgagga agatttgaat gacctagacg atggtgaatt ttactatcac gagattatcg
     2341 gtttggaagt ctatgagggt gatagcttgg ttggaaccat caaggaaatc ctgcaaccag
     2401 gtgctaatga tgtctgggtg gtcaaacgaa aaggcaaacg tgatttgctt ttaccttata
     2461 tcccaccagt ggttctcaat gttgatattc caaataaacg ggtcgatgtg gaaatcttag
     2521 aagggttaga cgatgaagat tgatatttta accctctttc cagagatgtt ttctccactg
     2581 gagcactcaa tcgttggaaa ggctcgagaa aaagggctct tggatatcca gtatcataat
     2641 tttcgagaaa atgctgaaaa ggcccgtcat gtagatgatg agccctacgg aggcggtcag
     2701 ggtatgttgc tcagagcaca acctattttc gattcctttg atgctattga aaagaaaaat
     2761 ccgcgcgtta ttctcctcga tcctgctgga aagcagtttg atcaggctta tgctgaagat
     2821 ttggctcaag aggaagagct aatctttatc tgtgggcact atgagggtta tgatgagcgc
     2881 attaagacct tggtaacaga tgagatttcc ctaggcgact atgtcctcac tggtggagaa
     2941 ttggcagcta tgaccatgat tgatgctaca gttcgcctga ttccagaagt gattggcaag
     3001 gagtctagcc accaagatga tagtttttct tcaggtcttt tagaatatcc tcagtacaca
     3061 cgtccctatg attatcgagg catggtcgtg ccagatgtat tgatgagtgg ccaccatgaa
     3121 aagattcgtc agtggcgatt gtacgagagt ttaaagaaaa cctacgagcg cagaccagat
     3181 ttacttgaac attatcaact gacagtagaa gaagaaaaaa tgctggcaga aatcaaagaa
     3241 aacaaagaat aaaggagaaa cctatgcaag taatcaaacg taatggcgaa attgctgaat
     3301 ttaatccaga taagatttac caagccatct tgaaggcagc ccaaactgtt tatgttttga
     3361 cagatgattt gcgtcaaaat cttgctcaag tcactaagaa ggtagttttg gatttacaag
     3421 aagccaaggt ggaacgtgcg actatcagta tgattcaatc tatggttgaa catcgtttat
     3481 tgggcgcagg ttacattacc attgcagaac actacatttc ctatcgttta caacgtgact
     3541 tggaaagaag tggttatgga gatcatatcg cagttcattt acattttgaa caaattcgct
     3601 aagaaaaaag agtgggatga agcaactaca tctcactctt tcttaaatct atttttttgg
     3661 gctgtttgga cagttgaggg gacaaatcgc aggcgttgaa tatcgcttat acggataatc
     3721 cggctcacat ttttggcaaa atttttcacg ataatttgct ggccttgctg atcatatttg
     3781 atgatatcac ctgtaaagct tgtctcagac aagataaggt gaacagcggt ttgtttctgg
     3841 atagcctctt caatagtggc tgtaagggag ttggtttcag tctggtcagg ttggtcatgt
     3901 ccatttaaaa aatcatgtat tttatctaga acttgctgga atttatgtct catattggcc
     3961 tcccttcttt tatatcaata ttatataaaa ttttcctaaa atctacaaga ttttacgaat
     4021 aaactaatga aactaaaaaa ggagaagaaa atggcagaat ttacatttga aatcgaagag
     4081 cacttgttga ctctttctga aaacgaaaaa ggttggacca aggaaattaa ccgtgtgagc
     4141 tttaatggtg cccctgcaaa gtttgatatt cgtgcttgga gtccagacca tactaaaatg
     4201 ggcaaaggga ttactctctc aaatgaagaa tttcaaacga tggtggatgc ctttaaaggc
     4261 aactaatact cttcgaaaat ctcttcaaac cgcatcagct ttgccttgcc gtatgtatgg
     4321 ttactgactt cgtcagtttc atccacaacc tcaaaacagt gttttgagca gcctgcggct
     4381 agcttcctag tttgctcttt gattttcatt gagtataagt tttcaaaata aaaaaaaagg
     4441 atattgagtc atgataactc ggtatccttt tttagttagt aaagtaagtc tgatttttaa
     4501 ggcgttgaag taggggacgg ctaataaaac taatagccag aagtttgcca aggtctggaa
     4561 tgataaaggg aagaaccccc acaacaagag ctttttcaaa tgccattcca gctaggaaat
     4621 gcaagctgag aatcccgccg acaaagacaa gggcatcacc caagaggttt gcaagaaaaa
     4681 tcttaacaac accactcttg ctgttggtta gagaggaagt aagtccagag taaacgagat
     4741 aaaaccaaag atagcctgca gtagggccaa ctaaagcctg aaaaccagct ccacctcctg
     4801 caaagacagg aagaccgata gcacctagaa gaagatagag tccagcagaa agtacagcct
     4861 ctctcggtct aaagacagta gcaatcaagc cgattgcaaa gttttgcaga gtgaagggaa
     4921 caggtccaat tggaagactg atttgtgcca aaacagcaat gagagcagcc ccaatagcag
     4981 ggatagcata aacgtgagct tttttcaaaa tagttaacct cttttctaat gtatagtaac
     5041 tattatactc atataggagt cgatgtcaac cattttttaa aaacaaggta acaaaaactg
     5101 taacagccga ttttcgtgac tagtcaagtt ggataatttt tttgttgatt cgaaaatcat
     5161 taaaaaagaa gcagacatat ttatatcagc ttcttttgtt tcgattaacg catggttaca
     5221 aattcttcag atgcagttgg gtgaattgcc acagttgcat caaagtcagc cttggttgct
     5281 cccattttga tagcaacggc aaatccctga atcatttcat caacgccgta gccaattcca
     5341 tgaagtccga caactttttc ttctgaacca gctgttatga gtttgaaacg ggattcttga
     5401 cggttgcgag tgcaagcaga gtacatagat gcaaagcttg atttataaac cttgatttgg
     5461 tcttgaccgt attctttaat agcttgctct tctgtcaaac caacagttcc gatagcaggg
     5521 tgtgaaaaga caacagttgg aatagttgag taatccattt ttgcagtagt ttttccgtta
     5581 aagagacgtt cagatagggt acgtccggcc ttgattgcaa ctggagtcag ttctttctcg
     5641 cccgttacat cacctagagc atagattccc tcaacaacag tattttggta ttcatccact
     5701 tggataaagc cacgttcgtt cagagtcact ccagcttttt caagttgcaa gcccttaacg
     5761 tttggacggc gacctgtagc ccagataact tggctagctg tgtgactagt accatcttcg
     5821 aaatgaatgg taatgccgtc agtagttttt tctaacttga cagggacttt gtgagtgtga
     5881 agtggtaagt ttgttctttc catttccttg accaaacctt caacgatgta ggaatcaaaa
     5941 ccacgtaaag gacgatcgcg gcgaacaaag agatctgtct tgacaccaaa agtgtggagt
     6001 acgccagcta attcaacggc gatataacca gcgcctagaa tggcaattga ctctggaagt
     6061 tcttcccagg caaatacatc atcagaagag ccacctagct cagcaccagg aatattagga
     6121 atacttggat gggcacctgt agcaatcacg atatgtttag cacgaatcag ttcaccattt
     6181 acgcttacag tatgagaatc tacaaattca gcatgacctt caatcaagtc tacaccgttg
     6241 cgtttaaaac taccatcata agaagaacga gcgcgatcaa tgtaggcttc acgattgcga
     6301 cgtagggttg caaagttaaa gttaagatca gtagtcttaa agccgtagtc ttctccaaat
     6361 tgatggaaag tctcagcgat ttgcgccccg taccacatga tttttttagg aacacaaccg
     6421 acgttgacac aggttccacc taatttcttt tcctcaataa cggctgcttt ggctccatgt
     6481 tcaccagcac ggttcatggt agcgattcct ccgctacctc caccgatagc aatgatatca
     6541 tattctctca ttgaaaactc ctttgtaatc ttttaaatag tagagtgtct tttttgatag
     6601 ttatatttta ttcttttttt gatttgattg caaaaatctg ctacgcacaa aaaattgcaa
     6661 aaaagcttgc caaataagtc aagatagtgt attatatatc tagtacaata gaaaagcaaa
     6721 attccgaacg attgtttaat cctgaactgc cattatttcc atctgaactg cattgtttgc
     6781 tggagggaaa tttggtataa tagcaaagta ttgtaaaaaa gaaaaggagt ttcattatga
     6841 agaaaaagaa tggtaaagct aaaaagtggc aactgtatgc agcaatcggt gctgcgagtg
     6901 tagttgtatt gggtgccggg gggattttac tttttagaca accttctcag actgctgtaa
     6961 aagatgagcc tactcatctt gttgttgcca aggaaggaag cgtggcatcc tctgttttat
     7021 tgtcagggac agtaacagca aaaaatgaac aatatgttta ttttgatgct agtaagggtg
     7081 atttagatga aatccttgtt tctgtgggcg ataaggtcag cgaagggcag gctttagtca
     7141 agtacagtag ttcagaagcg caggcggcct atgattcagc tagtcgagca gtagctaagg
     7201 cagatcgtca tatcaatgaa ctcaatcaag cacgaaatga agccgcttca gctcaggctc
     7261 cacagttacc agcgccagta ggaggagaag atgcaacggt gcaaagccca actccagtgg
     7321 ctggaaattc tgttgcttct attgatgctc aattgggtga tgcccgtgat gcgcgtgcag
     7381 atgctgcagc gcaattaagc aaggctcaaa gtcaattgga tgcaacaact gttctcagta
     7441 ccctagaggg aactgtagtc gaagtcaata gcaatgtttc taaatctcca acaggggcga
     7501 gtcaagttat ggttcatatt gtcagcaatg aaaatttaca agtcaaggga gaattgtctg
     7561 agtacaatct agccaacctt tctgtaggtc aagaagtaag ctttacttct aaagtgtatc
     7621 ctgataaaaa atggactggg aaattaagct atatttctga ctatcctaaa aacaatggtg
     7681 aagcagctag tccagcagcc gggaataata caggttctaa atacccttat actattgatg
     7741 tgacaggcga ggttggtgat ttgaaacaag gtttttctgt caacattgag gttaaaagca
     7801 aaactaaggc tattcttgtt cctgttagca gtctagtaat ggatgatagt aaaaattatg
     7861 tctggattgt ggatgaacaa caaaaggcta aaaaagttga ggtttcattg ggaaatgctg
     7921 acgcagaaaa tcaagaaatc acttctggtt taacgaacgg tgctaaggtc atcagtaatc
     7981 caacatcttc cttggaagaa ggaaaagagg tgaaggctga tgaagcaact aattagtcta
     8041 aaaaatatct tcagaagtta ccgtaatggt gaccaagaac tgcaggttct caaaaatatc
     8101 aatctagaag tgaatgaggg tgaatttgta gccatcatgg gaccatctgg gtctggtaag
     8161 tccactctga tgaatacgat tggcatgttg gatacaccaa ccagtggaga atattatctt
     8221 gaaggtcaag aagtggctgg gcttggtgaa aaacaactag ctaaggtccg taaccaacaa
     8281 atcggttttg tctttcagca gttctttctt ctatcgaagc tcaatgctct gcaaaatgta
     8341 gaattgccct tgatttacgc aggagtttcg tcttcaaaac gtcgcaagtt ggctgaggaa
     8401 tatttagaca aggttgaatt gacagaacgt agtcaccatt taccttcaga attatctggt
     8461 ggtcaaaagc aacgtgtagc cattgcgcgt gccttggtaa acaatccttc tattatccta
     8521 gcggatgaac cgacaggagc cttggatacc aaaacaggta accaaattat gcaattattg
     8581 gttgatttga ataaagaagg aaaaaccatt atcatggtaa cgcatgagcc tgagattgct
     8641 gcctatgcca aacgtcagat tgtcattcgg gatggggtca tttcgtctga cagtgctcag
     8701 ttaggaaagg aggaaaacta agatgcagaa tctgaaattt gccttttcat ctatcatggc
     8761 tcacaagatg cgttctttgc ttactatgat tgggattatt atcggtgttt catcagttgt
     8821 tgtgattatg gctttgggtg attccctatc tcgtcaagtc aataaagata tgactaaatc
     8881 tcagaaaaat attagcgtct ttttctctcc taaaaaaagt aaagatgggt cttttactca
     8941 gaaacaatca gcttttacgg tttctggaaa ggaagaggaa gttcctgttg aaccgccaaa
     9001 accgcaagaa tcctgggtcc aagaggcagc taaactgaag ggagtggata gttactatgt
     9061 aaccaattca acgaatgcca tcttgaccta tcaagataaa aaggttgaga atgctaattt
     9121 gacaggtgga aacagaactt acatggacgc tgttaagaat gaaattattg caggtcgtag
     9181 tctgagagag caagatttca aagagtttgc aagtgttatt ttgctagatg aggaattgtc
     9241 cattagttta tttgaatctc ctcaagaggc tattaacaag gttgtagaag tcaatggatt
     9301 tagttaccgg gtcattgggg tttatactag tccggaggct aaaagatcaa aaatatatgg
     9361 gtttggtggc ttgcctatta ctaccaatat ctcccttgct gcgaatttta atgtagatga
     9421 aatagctagt attgtctttc gagtgaatga taccagttta accccaactc tgggtccaga
     9481 actggcacga aaaatgacag agcttgcagg cttacaacag ggagaatacc aggtggcaga
     9541 tgagtccctt gtatttgcag aaattcaaca atcgtttagt tttatgacga cgattattag
     9601 ttccatcgca gggatttctc tctttgttgg aggaactggt gtcatgaaca tcatgctggt
     9661 ttcggtgaca gagcgcactc gtgagattgg tcttcgtaag gctttgggtg caacacgtgc
     9721 caatatttta attcagtttt tgattgaatc catgattttg accttgttag gtggcttaat
     9781 tggcttgaca attgcaagtg gtttaactgc cttagcaggt ttgttactgc aaggtttaat
     9841 agaaggtata gaagttggag tatcaatccc agtcgcccta tttagtcttg cagtttcggc
     9901 tagtgttggt atgatttttg gagtcttgcc agccaacaag gcatcgaaac ttgatccaat
     9961 tgaagccctt cgttatgaat gagatcaaca agatggacac tcgtctatct tgtttttgta
    10021 tggatttccc tattgaaatt cattaaaaat gtgatataat gaagtgttag aaaaacaggt
    10081 ttcatttgcc tggaaaggaa aaattatgtc tgaaaaaaat ttttatatta caacgccgat
    10141 ttactatcca tctgggaaac ttcatatcgg ttctgcctac acaactatcg catgtgatgt
    10201 cctagcacgt tacaaacgcc tgatgggcta cgatgtattt tatctgacag gtcttgatga
    10261 acatggtcag aaaatccagc agaaagcgga agaagctggt attacacctc aagcctatgt
    10321 tgatggaatg gcggttggag ttaaagaact ctggcaatta ctagatatct catacgataa
    10381 atttatccgt acaactgatg actaccatga aaaagttgtt gcacaggtct ttgaacgctt
    10441 acttgctcaa gatgatatct acttgggtga atactctggt tggtattcag tatcagacga
    10501 agaattcttt acagaaagcc agctggcaga agttttccgt gatgaagctg gaaatgtgac
    10561 tggtggtatt gctccatcag gtcatgaggt tgaatgggtt tctgaagaat catacttcct
    10621 tcgccttagc aaataccaag accgtttggt cgaatttttc aaagctcatc ctgaatttat
    10681 cacgccagat ggtcgcctta atgaaatgct acgcaacttc atcgagccag gtttggaaga
    10741 tttggcggta tctcgtacaa cctttacatg gggagtgcct gtcccatcaa atccaaaaca
    10801 cgttgtctac gtttggattg atgcccttct taactatgcg acagctcttg gctacgctca
    10861 agacgaacat ggtaactttg acaagttctg gaatggaaca gtcttccata tggtaggaaa
    10921 agatatcctt cgcttccact ctatctactg gccaatcctt cttatgatgt tggatgttaa
    10981 attacctgat cgtttgattg cccatggttg gtttgtcatg aaagacggaa aaatgtctaa
    11041 gtcaaaaggg aatgtcgttt accctgaaat gttggtagag cgttatggac tagatccact
    11101 tcgttactac ctcatgcgta accttccagt tggttcagac ggaaccttta ctcctgaaga
    11161 ctatattggc cgtatcaact atgaattggc taatgacctt gggaacctcc ttaaccgtac
    11221 ggtttccatg attaataagt actttgatgg acaaatccct gcctatgtag aaggtgtgac
    11281 tgaatttgat catgttcttg ctgaggttgc agaacaatca atcgcagact tccatacaca
    11341 catggaagca gttgactatc cacgtgcgct tgaagcagtc tggactctga tctctcgtac
    11401 caataaatac atcgatgaaa ccgcaccatg ggtcttggcc aaggatgaag ctcttcgtga
    11461 ccaattggca agtgtcatga gccacttggc agccagcatt cgtgtagttg ctcacttgat
    11521 tgaaccattt atgatggaaa ctagtcgtgc agttttgact caacttggtt tggaagaagt
    11581 ttctagtctt gaaaacttga gtttggctga cttcccagca gatgtgactg tagttgccaa
    11641 aggaacacct atctttccac gtctaaatat ggaagaagaa atcgcctata tcaaggaaca
    11701 aatggaaggc aataaaccag cagtcgaaaa agaatggaat ccggacgaag ttgagctcaa
    11761 actaaacaag gatgaaatca agtttgaaga ctttgacaag gttgaaatcc gtgtggcaga
    11821 agtcaaagaa gtgtctaaag tagaaggttc agataagttg cttcaattcc gcttggatgc
    11881 tggtgatgga gaagatcgtc agattctttc aggaattgcc aaatactatc caaatgaaca
    11941 agaattggtc ggcaagaaag ttcaaatcgt tgctaacctc aaaccacgta aaatgatgaa
    12001 aaaatatgtc agccagggta tgattctctc agctgaacat gatggcaaat taacccttct
    12061 cacagttgat ccagctgtac caaatggaag tgtgattggg taaaagtaga caggactagt
    12121 tcaggctagt tctgtttttt tatttatact caatgaaaat caaagagcaa actaggaagt
    12181 tagccgcagg ttgctcaaaa cactgttttg aggttgtaga tagaactgac gaagtcagct
    12241 caaaacatgt ttttgaggtt gtagatgaaa ctgacgaagt cagctcaaaa catgtttttg
    12301 aggttgtaga tagaactgac gaagtcagct caaaacatgt ttttgaggtt gtagatgaaa
    12361 ctgacgaagt cagctcaaaa catgtttttg aggttgtaga tgaaactgac gaagtcagct
    12421 caaaacatgt ttttgaggtt gtagatgaaa ctgacgaagt cagctcaaaa cacagttttg
    12481 aggttgtaga tgaaactgac gaagtcagta accctacata cggtaaggcg acgctgacgt
    12541 ggtttgaaga gattttcgaa gagtattaac tattatgctt tacaaactag tgtagaagtg
    12601 atatactata ggtgaagtta ctagtaggta ttacaaaata gattagaagg gagaagtgat
    12661 gaaagaaact cagctattaa aaggtgttct tgaaggttgt gtcttggata tgattggtca
    12721 aaaagagcgg tatggttatg agttggttca gactttgcga gaggctggat ttgatactat
    12781 cgttccagga actatttatc ctttgttgca aaagttagaa aaaaatcaat ggataagagg
    12841 cgacatgcgc ccgtcgccag atggtccaga tcggaagtat ttttcattaa cgaaagaagg
    12901 agaagagcgt gtctcagtct tttggcagca atgggacgat ttgagtcaaa aagtagaagg
    12961 gattaagaat gggggttaaa ccatgaagaa aatgaagtat tacgaagaaa caagcgcttt
    13021 gctacatgag ttttctgagg agaatcaaaa gtattttgag gagttgtggg aaagttttaa
    13081 tcttgctgga tttctctatg atgaagacta tctcagagag cagatctatt tgatgatgct
    13141 agatttctca gaagcagaac gagatggcat gagtgcagag gattatctag gtaagaatcc
    13201 taaaaaaata atgaaagaga ttctcaaggg agcacctcgc agttctatca aagagtccct
    13261 tttgacgcca attcttgtcc tggcggtatt acgttattat caactactaa gtgatttttc
    13321 taaaggtcct ctcttaacag tcaatttgct cacattttta gggcaacttc ttatttttct
    13381 gattggattt ggacttgtgg ccacaatttt acgaagaagt ttagtccaag attctcctaa
    13441 aatgaaaatt ggcacttaca ttgttgttgg gactatagtt cttctagttg ttttaggata
    13501 tgtaggaatg gcaagcttca tacaagaagg agccttttat attccggctc cctgggatag
    13561 tttgtctgtc tttacgattt cgctagttat cggtatttgg aattggaaag aagcggtctt
    13621 tcgtccattt gtcagtatga ttattgccca tcttgtggtg ggttctctgc tccgttatta
    13681 tgagtggatg ggaatttcaa atgttttcct tacaaaagtt attcctttag ctgtcctctt
    13741 tattggcatt tttctcttgt tccgtgggtt taagaagata aaatggagtg aagtatagtc
    13801 aaaaagccgt tgcaaagcgg ttttttgtta taatgaaggg aagaatgtta gaaattaaag
    13861 gagaaaaatt tgatgagata cataactctt ggtcaagatg acaaagaatt atcagaaatt
    13921 gttctcggaa tgatgagaat aaaagataag tctgtaaaag aagttgaaga gcttgtagaa
    13981 acagcacttt ctgttggaat caatgccttc gacttggctg atatttatgg tcgtggtcgt
    14041 tgtgaagaac tgttaggtct tgtcctaaaa aatcgtccag atttaagaga aaagatgtgg
    14101 attcagtcca aatgtggcat tcgcattgaa gaatttacct attttgattt ttctaaggac
    14161 tatattataa aatcagtaga cggtattttg caaagattga agattgatca tctagatagc
    14221 ttgctccttc atcgaccaga tgctttgatg gaatctgacc aagtagcaga agcctttgat
    14281 ctcctttata aacaaggtaa agttcgagat tttggagttt ctaatcaaaa tcctatgatg
    14341 atggagttgc ttaaaaaaga tgtcaagcag ccgttagctg ttaatcagct acaattgagt
    14401 gcggctttta ctccaggatt tgaatcagct tttcatgtta atatggaaga tagtcaagca
    14461 gctatgcgag atggcagcat ttttgaatat tgccaattac acgatgtggt cattcaagca
    14521 tggtctgtct tacaattcgg gtattttaaa gggaattttg ttggaaatga gaaatttcaa
    14581 gctttaaatc aagtacttga tcgtttagct attaaatatg gagtaacctc ttcaactatt
    14641 gccatttctt ggatattgcg ttatccagca aaaatgcagg cagttgtagg taccacaaat
    14701 cctaagcact tgagagaagt tagccaagcg gcaaacttta gcttaacaag aaaagaatgg
    14761 tatgaaattt atcttgctgc agggaataat ttgccgtaag tagaagaaga tgtaaataaa
    14821 tcacagtcaa aaagccgttt taaagcggtt tttttgttat aatgaagggg agaattattg
    14881 acattaaagg agaaaaacat gacatttgaa gaaattctac ctggcttaaa ggctaagaaa
    14941 aaatatgtac gaactggttg gggaggtgct gaaaactatg tccaactctt tgataccatc
    15001 gagcaaaatg ggcttgcact tgaagtgaca ccttatttcc taatcaacgt gtctggagaa
    15061 ggagaaggtt tttccatgtg gagcccgaca gcttgtgatg ttttggcaac ggattgggta
    15121 gaagtgcatg actaaacgtg tactcattac aggagtgagt tcagggatcg gattggctca
    15181 agctcgcctc tttttagaga agggctatca agtttatgga gttgaccaag gtgaaaagcc
    15241 actcttagag ggtgattttc actttttaca gagagatttg accttggact tggaacctat
    15301 ttttgactgg tgccctcagg tggatgtttt gtgtaatact gccggagttt tggatgatta
    15361 caaaccactg ttggaacaaa cggcgcagga cattcaagag atttttgaaa tcaactacat
    15421 tattccagta gagttgactc ggtattattt gacacaaatg ctggaaaata aaaagggaat
    15481 catcatcaat atgtgttcca ttgcttctag cctagcaggt ggaggtggtc acgcctatac
    15541 ttcatccaag catgccttgg ctggcttcac caagcagttg gctctagact atgctgaagc
    15601 tgggattcag gtctttggta tcgctccagg agcagtcaag acagctatga ccgctgcgga
    15661 ttttgagaca ggtggcttag ctgactgggt ggctagtgaa acaccaatca agcgctggat
    15721 tgaaccagag gaagtggcag aagtcagtct ctttttggcc agtggaaagg catctgccat
    15781 gcagggacaa attctgacaa tagatggtgg ctggtctttg aagtaggagg agttggatgg
    15841 aaatcaaaaa tcactttggg gtctactgtg tttgctttga aaatggaaag ttactttgca
    15901 ttgaaaaaac gagaggccct tatcaacatc ggtatgatct acctggaggc agtcagcaac
    15961 ttggtgaagg actgacggaa acgctgacta gagaagttat ggaagagacg ggatttactg
    16021 ttagaagcta ctctaatcct cgaatctacg atgttttcgt cagggaagag ttaaaaaatt
    16081 ttatggttca ccatgtcata gccttgtatg atgttgaaat gaatgagagt gcacctcaag
    16141 ttacgatttc ggaagctgtg tctgatggtg cgaatgattc acttggatat atttggatgg
    16201 atattcaaga gattacagaa gaaaatgcat cgccattagt cttgaaggtt aagtctgaat
    16261 tattaggatt gccagaactg gacaagactt cttacatgaa ttggaaggtg aatgatgaga
    16321 aatcaacttg cccttagtgg cgaaaaaatt cttgaaaaaa tttatcctca actatttcac
    16381 cacgtcggca tgattcgtgg ggaatactta ttgagagagc ttaatcaaaa catcatatta
    16441 gcaagttgtc agcaatttgt aaaagattat cttgatacta tttgctcctt ttacttagat
    16501 aaggaagttt ggtatcgttt ttctgaatta acgaatacag aagccaattg tttagcgggg
    16561 actagagagt tttttgacga agggcatccc ttgtttggct atagaggaac caggcgttta
    16621 ctggcgtgtt tggatgaatt tcaggctgaa tcacatgtcg ttacagaagt ttatcaaacc
    16681 aatcccaatc tgtctcttat ttttcctttt gtcaatgatg ccgaccaatt aaaacaagct
    16741 attacagtat tgcgtcagta tggctttact gggaaagtcg gaacgatgat tgaattaccg
    16801 tcagcgtatt ttgacttatc tagtatactg gaaacgggca tttcaaagat tgtagttgga
    16861 atgaatgatt tgacttcctt tgtttttgcg actatgagaa acagtcaatg gcatgatatg
    16921 gaaagtccaa taatgttaga tatgctaaga gatatgcagg ataaagcaag aaagaacaag
    16981 attaattttg ctgtagcagg ctatctgaat acttctttca tacaaaaaat gaatcagttg
    17041 ggtatcaagt gcattatcca ctacagttct attccagaga tttttgattt agagattgac
    17101 catccagatc atcttaaaca cataaaagaa gaaagtaaaa aattacaaag gagtacccat
    17161 gataccgcaa gaaatgtgga ataaatacaa gcaaatcaat cctttgatcg gagatgaaat
    17221 cgatgcttgg gcttttggag ttgaaccaga ccttttagcg gatttggtgt ttaaaggaga
    17281 aaagacagca acagcatcag cctatgacct ctatgtacta gaagacgaac cacttcccca
    17341 agtagggacc ttcgatgtca ttttagatag tcaaaatcag gctgtctgcg ttgtcgaaat
    17401 tacaaaggtt tctgttgaac tcttcaatca agtttctgcg caacatgcct ttaaggaagg
    17461 tgagggagac aaatcacttg cctattggcg ccaggttcat gaggactttt tcacagactg
    17521 tttgggtgaa gtagggctga cttttacacc tgaaagcaag gttgttttag aagaatttcg
    17581 caaggtctac ccactgtaga ctattagaag gaagaaagtt ttggaaatcg ctgtccaatc
    17641 cttttttctc aagcaaaata tgatataata agtttgtttg aagaagagca gcagctctta
    17701 aacttagaat aggagaaaac tatgcaagca gttgaacatt ttattaagca atttgttcct
    17761 gaacattatg atttattttt agatttgagt cgtgagacca agactttttc tgggaaagtg
    17821 accatcactg gtcaagcaca gagtgaccgc atctccctcc accaaaaaga cttggaaatc
    17881 acctctgtag aagttgcagg tcaagctcgt ccatttacag ttgaccatga caatgaagcc
    17941 cttcatatcg aattggctga ggctggtcaa gttgaattgg ttcttgcctt ttctggtaaa
    18001 attacagaca acatgacagg gatttaccct tcttattata cagttgatgg agtcaagaag
    18061 gaggtcttgt ctactcagtt cgagagccat tttgcgcgcg aagctttccc atgtgtggat
    18121 gagcctgaag ccaaagcaac ttttgacctc tctcttcgct ttgaccaagc agaaggtgaa
    18181 ttggccttgt caaacatgcc agaaatcgat gttgaaaacc gtaaggaaac aggtatctgg
    18241 aagtttgaga caacacctcg catgtcttct tacttgttgg cctttgttgc tggtgatttg
    18301 caaggggtga ccgctaaaac taaaaatggt accctggtag gtgtctactc aaccaaagca
    18361 catccacttt caaatcttga tttctcactg gatatcgctg ttcgctctat cgagttttac
    18421 gaagattact atggagttaa gtacccaatt cctcaatctc tccacatcgc ccttcctgac
    18481 ttctcagctg gtgctatgga aaactggggt cttgtgacct accgtgaagt ttacttggtt
    18541 gtcgatgaga actctacatt tgctagccgt caacaagttg cccttgttgt ggcccatgaa
    18601 ttggctcacc aatggtttgg gaacctcgtg actatgaaat ggtgggatga cctttggctc
    18661 aatgaaagtt tcgctaatat gatggaatac gtctgtgtgg ataccatcga accaagctgg
    18721 aatatctttg aagatttcca aacaggtgga gtacctcttg ctcttgaacg tgacgctact
    18781 gatggcgttc agtctgtcca cgtcgaagtt aaacatccag atgaaatcaa tacactcttt
    18841 gacggcgcta tcgtctatgc caaaggaagc cgtctcatgc acatgcttcg ccgttggcta
    18901 ggagatgctg attttgctaa aggtttgcac gcctactttg aaaaacacca atacagcaac
    18961 accattggta gtgacctttg ggatgccctt ggtcaagcgt caggacgtga tgtcgcagcc
    19021 ttcatggatt cttggttgga acagcctggt tacccagttc ttactgtcaa agttgaaaat
    19081 gatgtcttga agatttcaca aaaacaattc ttcatcggtg agaacgaaga caagaaccgt
    19141 ctctgggtgg tgcccctcaa tagcaactgg aaaggcttgc ctgatacact cgaaactgaa
    19201 agtatcgaaa tccctggcta cgcagctctt cttgctgaga atgaaggagc tcttcgtctc
    19261 aacactgaaa atactgccca ctatattacc gactatcaag gagacttgtt agaagctgtt
    19321 cttgctgagc tagagacact tgataacaca agcaaactgc aaattgttca agaacgtcgt
    19381 ttgttggctg aggcagggca catttcttat gcagacttgc ttccagttct tgataaactt
    19441 gctaaggaag agtcttatct ggtggtttca gctgtttctc aagtgatttc agcccttgag
    19501 cgctttattg atgaaggaac ggatgctgaa acagccttca aaggactggt tgctaaattg
    19561 gctcgtcata actatgaccg tcttggtttt gaagctaaag acggagaatc agatgaggat
    19621 gaattggttc gtcagctggc tgtttcgatg atgattcgct caaatgatgc agaagctagt
    19681 caagtcgcta gccaaatctt cgcaacacac aaggagaatc ttgcaggact cccagcagct
    19741 attcgctcac aagttctgat taatgagatg aaacatcatg agactaaaga cttgttagca
    19801 ctttatcttg atacttatac tcacgcaaca gatgctgtct ttaaacgcca gttggcagca
    19861 gctcttgcat acagtacaga tgcggataat atccaaaact tgattacttc ttggaaggac
    19921 aaatttgtgg tcaaaccaca ggacttgtct gcttggtatt accaattcct agctcatcaa
    19981 gcaactcaga aaacagcttg gtcttgggcg cgtgaaaact gggcttggat taaggcagcc
    20041 cttggaggag atatgagctt tgatagcttt gttatccttc ctgctcatgt atttaagact
    20101 cagcaacgct tggcggaata caaggagttc tttgaaccgc aactttctga ccttgctctt
    20161 agccgtaaca tcggtatggg aatcaaggaa attgcagcgc gtgttgactt gattagccgt
    20221 gaaaaagctg cagtcgaagc agttgttctt caatacggaa atgcataaat aagcctaaaa
    20281 taaaaagaaa actcagctat ctcatgtaaa atgcagagag ttgagttttt tttaaggcaa
    20341 atttggtata atagttttaa taaggaggag tttctcatga taaaaatctt attggttgag
    20401 gatgacctag gtctgtcaaa ttcagtattt gactttttag acgattttgc ggatgttatg
    20461 caggtatttg atggagaaga aggtctctac gaagctgaga gtggtgtcta tgacttgatt
    20521 ttgctggatt tgatgttgcc agaaaaaaat ggtttccaag tcttaaaaga attgcgtgaa
    20581 aagggaatta cgacaccagt tctgattatg actgccaagg aaagtttgga tgacaaggga
    20641 catggatttg aactgggagc ggatgattat ctgaccaaac ctttctacct agaagaactt
    20701 aaaatgcgga ttcaggccct tctcaaacgt tcagggaagt ttaatgaaaa caccttgact
    20761 tatgggaata tcgtggttaa tttatcaacc aataccgtta aagttgaaga tactcctgtc
    20821 gaattgctgg ggaaagagtt cgatttacta gtttatttcc ttcaaaatca aaatgtgatt
    20881 ttgcctaaga cgcagatttt tgaccgtcta tggggatttg atagtgatac aacgatttcg
    20941 gttgtcgaag tctatgtttc aaaagtccgt aagaaattaa agggaaccac ttttgcagag
    21001 aatttgcaaa ccttgcgtag tgttgggtat cttttaaaag atgttcagta aacttaaaaa
    21061 aacatggtat gcggatgact ttagttattt tatccgcaac ttcggtgtct tcaccctgat
    21121 tttttctaca atgactctga ttattttaca agtcatgcat tcgagtcttt atacttcggt
    21181 ggacgataag cttcatggac tgagtgaaaa tcctcaagca gttattcagc tggctataaa
    21241 tagggcaaca gaagagatta aagatttaga aaatgctagg gcggacgcta gtaaagtaga
    21301 aataaaacct aatgtcagtt ccaatacgga agtcattctc tttgataaag actttactca
    21361 acttctttct ggaaatcgat ttttgggctt ggataagatt aagttagaaa agaaagaact
    21421 aggacatatc taccagattc aggtttttaa tagctatggg caggaagaaa tctatcgtgt
    21481 gattttgatg gagaccaata ttagttcggt ttcaaccaat atcaagtatg ctgctgtctt
    21541 gattaatacc agtcagttgg agcaggctag tcaaaagcat gagcaattga ttgtggtcgt
    21601 gatggctagt ttctggattt tgtctttact tgccagtctc tatctagcta gggtcagtgt
    21661 taggcccttg cttgagagta tgcagaagca acagtctttt gtggaaaatg ccagtcatga
    21721 gttacgaact ccactcgcag ttttgcaaaa tcgcttagag accctttttc gtaagccaga
    21781 agctaccatt atggatgtga gcgaaagcat tgcatcgagt ttggaagaag tccgaaatat
    21841 gcgtttttta acgacaagct tgctgaactt agctcggaga gatgatggga ttaagccgga
    21901 gcttgcagaa gttccaacta gcttttttaa tacaactttc acaaactatg agatgattgc
    21961 ttcggaaaat aatcgtgtct tccgttttga aaatcgtatc catcgaacaa ttgtcacaga
    22021 tcagcttctt ctgaaacaac tgatgaccat tcttttcgat aatgccgtca agtatactga
    22081 ggaggatggt gaaattgatt ttcttatctc ggcgaccgat cgcaatcttt atttacttgt
    22141 ttctgataat ggaatcggta tttcgacaga agataaaaag aaaatttttg accgttttta
    22201 tcgagtagac aaggctagaa cccggcaaaa aggtggtttt ggtttaggat tatccctagc
    22261 caagcaaatt gtagatgctc taaaaggaac tgttactgtc aaagataata aacccaaggg
    22321 aacaatcttt gaagtgaaga ttgccattca gacaccatct aaaaagaaaa aataaaaata
    22381 tcgctccaat tggggcgata ttttggattt atcttctacg ttttcgtttg ataatagacc
    22441 gttgaacttt taaaacaagt aagctgaatc cgattgctgc ggcaaaggca agagcagttg
    22501 ataattttaa tgctaaaaag ataaaactaa agatagcaat acagatacaa aaaacagcga
    22561 tattaataaa aaataggatt tccttgagat tggcatcaga ttgcgcttca ggtgtataag
    22621 cttggtaatg aggaagctgc tggtttaatt cttcttgata gtctacctca taggattgta
    22681 attttcttac gggcatgatt ctctccttaa cagtacatac ctattttatc attttttcgg
    22741 cagagaatta ttacagaaag gttacaaaaa gaataaagtc ccttttcatt ttcaaagcat
    22801 ggctgatttt ggagaaatgt ggtataattt ttcttatgga aaagattgtc attacagcaa
    22861 ctgctgaaag tattgaacaa gttgaacaac tactcgaagc tggcgtagac cgtatctatg
    22921 tcggtgagaa agattttggc cttcgtctgc caacgacctt tagttatgac caattacgtg
    22981 aaatcgctaa gttggttcat gatgctggta aggaattgat cgttgcggtc aatgctctca
    23041 tgcaccaaga tatgatggac cgtatcaagc ctttcttaga cttcttggaa gaaatcaaga
    23101 cagactatat tacgattggg gatgcaggcg tcttttacgt agttaaccgc gatggttatt
    23161 catttaagac catctacgat gcttcaacca tggtaactag cagtcgtcag attaacttct
    23221 ggggacaaaa ggctggcgca tctgaggctg ttttggcgcg tgaaattcca tcagctgaac
    23281 ttttcaaaat gccagagatt ttggaaattc ctgctgaagt tttggtttac ggtgctagcg
    23341 tcatccatca ttctaaacgt ccactcttgc aaaactacta taactttaca catatcgatg
    23401 atgaaaagac gcataaacgt gacctcttct tggctgagcc aagtgatcca gagagccact
    23461 attccatttt tgaagataat catgggaccc atatctttgc caacaatgac cttgatttga
    23521 tgatcaaatt aacagaattg gtggagcatg gctttactcg ctggaaacta gaagggctct
    23581 acactcctgg tcagaacttt gttgagattg caaaactctt tatccaagcg cgtagcttga
    23641 ttcaagaggg caactttagt catgctcaag ccttcttgct ggatgaagaa gttcgtaaac
    23701 ttcaccctaa aaaccgtttc cttgatacag gattttatga ctacgatcct gacatggtta
    23761 gataaaatac atgattcgtt gagagaagga agatgcaaac atttcttctc tcaatttttc
    23821 gtatttcttc actattttac aaaaatcagc aggctagaat gctctattcg atgggatttt
    23881 taagaaaagt agtgttcttg agtttgaaaa ttatcctatg tttgcaggtg ccaaatggcc
    23941 ctttttttgg tataattttt tataatgaaa acgattggta atcgctatgt tgtggtggat
    24001 ttagaggcaa ctagcacagg tagtaaggct aaaattatcc aagtgggaat tgtcgtgatt
    24061 gaggacggag aaatcgtcga tcactatacg acggatgtca atccacatga acccttggat
    24121 gctcatatca aagaactgac aggattgaca gaccaacgtc tggcgcaagc acctgatttt
    24181 tcgcaagttg ccagaaaaat atttgacttg gtggaggatg ggatttttgt agcccataat
    24241 gttcagtttg atgctaatct cttggcggaa aatttatttt ttgaaggcta tgagctaaga
    24301 aaccctcgtg ttgatacggt cgaattggcc caggtctttt tccctgaact ggaaaaatat
    24361 agcttgccga ttttgtgtcg agaattagga attcctctta aacacgcaca cacagccctt
    24421 tcagatgccc aagctacagc agaattactt ctttttttac ggaaaaagat gacccagctt
    24481 cctaaaggtc tcttggaacg cttgctggaa atggctgacg ctctcctata tgagtcctac
    24541 ctggttattg aggaaactta tcgcaaccaa tctatcctga gttctccaga cttggtccaa
    24601 gttcaaggtc tatattttaa gaaaacggaa gcttctctgg agccacgaaa actatctcaa
    24661 gacttttcta aaaatatttc tctgttgaac cttgaagtga gggaggaaca agaaagtttt
    24721 gctaaagagg ttggcttgct attgaaagat gaacctgtct ctctgattca agcgccgaca
    24781 gggattggga aaacctatgg ctatctctta cccgctttat ctcaatccaa agagcgacaa
    24841 attgttctta gtgttccgac aaagattctt caaaatcaaa tcatggaaga agaaggtaaa
    24901 cgcctcaagg aagtgttcca tacagatatt catagcttaa agggaccaca aaattatctg
    24961 aagttggatg ccttttatca ttccttgcag gaaaatgatg aaaatcgctt atttagacgc
    25021 tttaaaatgc aagtcttggt ctggcttact gagacagaga caggagattt ggatgaaatc
    25081 gggcaactct accgttacca acattttcta gcagaccttc gtcatgatgg gaatttatca
    25141 tcccagagct tatttgtgac ggaagatttt tggaaacgta gtcaagaaag ggcagagact
    25201 tgcaagcttt tagtgactaa tcatgcctat ctcgtaacca gacttgaaga taatcctgaa
    25261 tttgtcagtg accgtttact gattattgat gaagtccaaa agattttgtt agctctagaa
    25321 aatctgcttc aagagaccta cgatatacaa tctattatcg atttaattga taaggcttta
    25381 gtaggagaag aaaacagggt tcaacaacgg atactagaaa gtattcgctt tgagtgtctc
    25441 tacttgatag aacaatttca gtctggcaaa tctaggaaaa atatcttaga ttctctggac
    25501 aatctccatc agtatttttc agaattggaa gtagaagact ttgatgagct ggttcgctat
    25561 tttacagctg aaggtgatta ctggcttgaa gtaactgaaa cgagtcaaaa gaaaattcag
    25621 atttcttcta caaaatcagg ccgtactctt ctgtcctctt tacttcctga gagttgccaa
    25681 gtcttgggag tatcggctac tcttgagatt agtcagaggg tttctttggc agacctttta
    25741 ggctatcctg aagctaaatt tgtcaagatt gaatctcggg gaaaacagga acaagaagtg
    25801 gtcatggtca aagatttccc tctggtaaca gaaaactcct tagaagtcta tgccagagag
    25861 gtagctgctt tactagtgga aattcaagct ttccagcaac cgattttggt tctctttacc
    25921 gctaaagaca tgcttctagc agtatcggat ttacttacag ttagccactt ggcccagtat
    25981 aaaaatgggg atgttcatca gctaaagaaa cgctttgaaa aaggtgaaca acaaatcttg
    26041 cttggtgcag caagtttctg ggagggagtt gatttttcaa gccatccttc tgtgattcaa
    26101 gttgtaccga ggcttccttt ccaaaatcct caagaaccct tgacgaaaaa gattaatcaa
    26161 gaactgaatc aagaagggaa aaatgccttt tatgattatc aattgccaat ggccattatt
    26221 cgtttaaaac aggctttggg aagaagtatg agacgtgaat accaacgttc cttaactctt
    26281 attttggata ggagaatcgt cggaaaacga tacggtaaac aaatagtagc atctctagca
    26341 gaagaagcaa ctgttaaaac catctctcga tccgaagttg acgaggctat tgatagattt
    26401 tttaatgaac tttgataaat agtattgtat gaaagtataa ggttagtata tatgaaacgt
    26461 tctctcgact ctagagtcga ttatagtttg ctcttgccag tattttttct actggtcatc
    26521 ggtgtggtgg ctatctatat agccgttagt catgattatc ccaataatat tctgcccatt
    26581 ttagggcagc aggtcgcctg gattgccttg gggcttgtga ttggttttgt ggtcatgctc
    26641 tttaatacag aatttctttg gaaggtgacc ccctttctat atattttagg cttgggactt
    26701 atgatcttgc cgattgtatt ttataatcca agattagttg catcaacggg tgccaaaaac
    26761 tgggtatcaa taaatggaat taccctattc caaccgtcag aatttatgaa gatatcttat
    26821 atcctcatgt tggctcgtgt cattgtccaa tttacaaaga aacataagga atggagacgc
    26881 acggttccgc tggacttttt gttaattttc tggatgattc tctttaccat tccagtccta
    26941 gttcttttag cacttcaaag tgacttgggg acggctttgg tttttgtagc cattttctca
    27001 ggaatcgttt tattatcagg ggtttcttgg aaaattatta tcccagtatt tgtgactgct
    27061 gtaacaggag ttgctggttt cttagctatc tttattagca aggacggacg agcttttctt
    27121 caccagattg gaatgccgac ctaccaaatc aatcggattt tggcttggct caatcccttt
    27181 gagtttgccc aaacaacgac ttaccagcag gctcaagggc agattgccat tgggagtggt
    27241 ggcttatttg gtcagggatt taatgcttcg aatctgctta tcccagttcg agagtcagat
    27301 atgattttta cggttattgc agaagatttt ggctttattg gctctgtcct ggttattgcc
    27361 ctctatctca tgttgattta ccgtatgttg aagattactc ttaaatcaaa taaccagttc
    27421 tacacttata tttccacagg tttgattatg atgttgctct tccacatctt tgagaatatc
    27481 ggtgctgtga ctggactact tcctttgacg gggattccct tgcctttcat ttcgcaaggg
    27541 ggatcagcta ttatcagtaa tctgattggt gttggtttgc ttttatcgat gagttaccag
    27601 actaatctag ctgaagaaaa gagcggaaaa gtcccattca aacggaaaaa ggttgtatta
    27661 aaacaaatta aataaggaga aaatcatggt aaaagtagca gttatgttag ctcagggctt
    27721 tgaagaaatt gaagccttga cagttgtaga tgtcttgcgt cgagccaata tcacatgtga
    27781 tatggttggt tttgaagagc aagtaacggg ttcgcatgca atccaagtaa gagcagatca
    27841 tgtctttgat ggaaatttat cagactatga tatgattgtt cttcctggag gtatgcctgg
    27901 ttctgcacat ttacgtgata atcagacctt gattcaagaa ttgcaaagct tcgagcaaga
    27961 agggaagaaa ctagcagcca tttgtgcggc accaattgcc ctcaatcaag cagagatatt
    28021 gaaaaataag cgatacactt gttatgacgg cgttcaagag caaatccttg atggtcacta
    28081 cgtcaaggaa acagtagtgg tagatggtca gttgacaacc agtcggggtc cttcaacagc
    28141 ccttgccttt gcctacgagt tggtggagca actaggaggg gacgcagaga gtttacgaac
    28201 aggaatgctc tatcgagatg tctttggtaa aaatcagtaa aacgggagtt attctctcgt
    28261 tttttatgtg gaaaactcag ggaaatcatc gcttttttca taaaaaaatg ctataatgaa
    28321 gggtatgaaa tatcacgatt acatctggga tttaggtgga actttactgg ataattatga
    28381 aacttcaaca gctgcatttg ttgaaacatt ggcactgtat ggtatcacac aagaccatga
    28441 cagtgtctat caagctttaa aggtttctac tccttttgcg attgagacat tcgctcccaa
    28501 tttagagaat tttttagaaa agtacaagga aaatgaagcc agagagcttg aacacccgat
    28561 tttatttgaa ggagtttctg acctattgga agtcatttca aatcaaggtg gccgtcattt
    28621 tttggtctct catcgaaatg atcaggtttt ggaaatttta gaaaaaacct ctatagcagc
    28681 ttattttaca gaagtggtga cttctagctc aggctttaag agaaagccaa atcccgaatc
    28741 catgctttat ttaagagaaa agtatcagat tagctctggt cttgtcattg gtgatcggcc
    28801 gattgatatc gaagcaggtc aagctgcagg acttgatacc cacttgttta ccagtatcgt
    28861 gaatttaaga caagtattag acatataaga aaaaggaata agatgacaga agaaatcaaa
    28921 aatctgcagg cacaggatta tgatgccagt caaattcaag ttttagaggg cttagaggct
    28981 gttcgtatgc gtccagggat gtacattgga tcaacctcaa aagaaggtct tcaccatcta
    29041 gtctgggaaa ttgttgataa ctcaattgac gaggccttgg caggatttgc cagccatatt
    29101 caagttttta ttgagccaga tgattcgatt actgttgtgg atgatgggcg tggtatccca
    29161 gtcgatattc aggaaaaaac aggccgtcct gctgttgaga ccgtctttac agtccttcac
    29221 gctggaggaa agttcggcgg tggtggatac aaggtttcag gtggtcttca cggggtgggg
    29281 tcgtcagtag ttaatgccct ttccactcaa ttagacgttc atgttcacaa aaatggtaag
    29341 attcattacc aagaataccg tcgtggtcat gttgtcgcag atcttgaaat agttggagat
    29401 acggataaaa caggaacaac tgttcacttc acaccggacc caaaaatctt cactgaaaca
    29461 acaatctttg attttgataa attaaataaa cggattcaag agttggcctt tctaaatcgc
    29521 ggtcttcaaa tttcaattac agataagcgt gaaggtttgg aacaaaccaa gcattatcat
    29581 tatgaaggtg ggattgctag ttacgttgaa tatatcaacg agaacaagga tgtaatcttt
    29641 gatacaccaa tctatacaga cggtgagatg gatgatatca cagttgaggt agccatgcag
    29701 tacacaactg gttaccatga aaatgtcatg agtttcgcca ataatattca tacacatgaa
    29761 ggtggaacgc atgaacaagg tttccgtaca gccttgacac gtgttatcaa tgattatgct
    29821 cgtaaaaata agttactgaa agacaatgaa gacaacctaa caggggaaga tgttcgcgaa
    29881 ggcttaactg cagttatctc agttaaacac ccaaatccac agtttgaagg acaaaccaag
    29941 accaaattgg gaaatagcga agtggtcaag attaccaatc gcctcttcag tgaagccttc
    30001 tccgatttcc tcatggaaaa tccacagatt gccaaacgta tcgtggaaaa agggatttta
    30061 gctgccaagg ctcgtgtggc tgccaagcgt gcgcgtgaag tcacacgtaa aaaatctggt
    30121 ttggaaattt ccaaccttcc agggaaacta gcagactgtt cttctaataa ccctgctgaa
    30181 acagaactct tcatcgtcga aggagactca gctggtggat cagccaaatc tggtcgtaac
    30241 cgtgagtttc aggctatcct tccaattcgc ggtaagattt tgaacgttga aaaagcaagt
    30301 atggataaga ttctagctaa cgaagaaatt cgtagtcttt tcacagccat gggaacagga
    30361 tttggcgcag aatttgatgt ttcgaaagcc cgttaccaaa aactcgtttt gatgaccgat
    30421 gccgatgtcg atggagccca cattcgtacc cttcttttaa ccttgattta tcgttatatg
    30481 aaaccaatcc tagaagctgg ttatgtttat attgcccaac caccaatcta tggtgtcaag
    30541 gttggaagcg agattaaaga atatatccag ccgggtgcag atcaagaaat caaactccaa
    30601 gaagctttag cccgttatag tgaaggtcgt accaaaccga ctattcagcg ttataagggg
    30661 ctaggtgaaa tggacgatca tcagctgtgg gaaacaacca tggatcccga acatcgcttg
    30721 atggctagag tttctgtaga tgatgctgca gaagcagata aaatctttga tatgttgatg
    30781 ggggatcgag tagagcctcg tcgtgagttt atcgaagaaa atgctgtcta tagtacactt
    30841 gatgtctaaa aaatagggag aagtagttcc cttggtctaa aaaatatgat ataatcgtta
    30901 cgattgtttc aagtaaaaaa ggagtttgat atgtctaatg gacaactaat ttatttaatg
    30961 gttgcaattg cagtcatttt agttctggct tatgtagtgg caatctttct acgtaagcga
    31021 aacgagggga gattagaggc gctagaagaa agaaaagaag aactatacaa tcttccagta
    31081 aatgatgaag tagaagctgt aaaaaatatg cacttgattg gacaaagtca agtggctttc
    31141 cgtgaatgga atcaaaaatg ggtcgattta tctctcaact cttttgccga tattgaaaat
    31201 aatctctttg aagcagaagg ctataaccat tcatttcgtt ttctcaaggc cagtcatcaa
    31261 attgaccaaa ttgagagtca aattactttg attgaagaag atattgcggc aattcgcaat
    31321 gctttggcag acttagagaa gcaagaatct aaaaatagtg gtcgtgttct tcatgctttg
    31381 gatttatttg aggaacttca gcatagagtt gctgaaaatt cagaacagta tggtcaagcc
    31441 ttggatgaaa ttgaaaaaca attagaaaat atccaatctg aattttcaca atttgtaacc
    31501 ttgaattcat cgggtgaccc tgtggaagcc gcagtgattt tggataatac agaaaatcac
    31561 attttggcct taagtcatat tgtggatcgt gttccagcct tggttacgac gctttctaca
    31621 gaattgccag atcaattaca ggatttggaa gccggttatc gtaaactaat tgatgctaat
    31681 tatcattttg ttgaaacgga tattgaagcg cgtttccact tgctttatga agcattcaag
    31741 aaaaaccaag agaatattcg tcagttggaa ttggataatg ccgaatatga gaatggacag
    31801 gcacaagagg aaatcaatgc cttgtatgat atttttactc gagaaattgc tgctcagaaa
    31861 gtagtggaaa atctacttgc aactcttcca acttaccttc aacatatgaa agagaataat
    31921 actttattgg gagaagatat tgcacgtttg aacaagacct atttacttcc tgagacagct
    31981 gcaagccatg ttcgtcgtat tcagacagaa ttagagagtt ttgaggcagc tattgttgag
    32041 gtaacttcga atcaagaaga accaacccaa gcttattcag ttcttgaaga aaatcttgag
    32101 gatttacaaa ctcaactaaa agatattgaa gatgagcaaa tttcagttag tgagcgcctg
    32161 acacaaattg agaaagatga tattaatgca cgtcaaaagg ccaatgttta tgtcaatcgt
    32221 ctccatacta tcaagcgata catggaaaaa cgcaatctgc caggtattcc acaaactttc
    32281 ttgaagttat tctttacggc aagcaataat accgaggatt taatggttga gttagaacaa
    32341 aaaatgatta acattgaatc tgttacccga gttcttgaaa ttgcaacgaa tgatatggaa
    32401 gctttagaaa cggaaactta taatattgta caatatgcaa ctttgacaga gcaactcttg
    32461 caatattcta accgctatcg ctcatttgat gaacgcattc aagaagcatt taacgaagct
    32521 ttagatattt ttgaaaaaga atttgattat cacgcttcat ttgacaagat ttctcaagca
    32581 ttggaagtgg cagagcctgg tgtaaccaat cgctttgtta cctcatatga gaaaacacgt
    32641 gaaacgattc gtttttaata aaagaaaaag attttattgt gtgaggagca gaatcaaatc
    32701 tttttctata gttgtgggga gatttacttc attttctcct gagattgagt ttttgcccag
    32761 ccgatttatc caccacctca aaacagtgtt ttatactctt cgaaaatctc ttcaaatcac
    32821 gtcagcgtcg ccttaccgta ctcaagtaca gcctgaggct cttagtttgc tttttgattt
    32881 tcatttagta ttaaagtgat ttcgtcagtc ttatctgcag cttcaaatct gtactttgag
    32941 taacttggta accgtccaat aacgaagtct attgaaaaat ctccagacta gagaactcac
    33001 ggatagttcc
//
LOCUS       DAVGJL010000009        32662 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.009, whole genome shotgun sequence.
ACCESSION   DAVGJL010000009 DAVGJL010000000
VERSION     DAVGJL010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 32662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32662
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.009"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..147
                     /locus_tag="VVE42_000354"
     CDS             <1..147
                     /locus_tag="VVE42_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEU3072616.1"
                     /translation="AKLEATNNLIKLIKRNAFGFRNFENFKKRIFIALNIKKERTKFV
                     LSRA"
     gene            complement(235..924)
                     /gene="rplA"
                     /locus_tag="VVE42_000355"
     CDS             complement(235..924)
                     /gene="rplA"
                     /locus_tag="VVE42_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEU3072617.1"
                     /translation="MAKKSKQLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKAKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
     gene            complement(1132..1557)
                     /gene="rplK"
                     /locus_tag="VVE42_000356"
     CDS             complement(1132..1557)
                     /gene="rplK"
                     /locus_tag="VVE42_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEU3072618.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFVTKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAMRMIEGTARSMGFTVVD"
     gene            1689..1826
                     /locus_tag="VVE42_000357"
     CDS             1689..1826
                     /locus_tag="VVE42_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3072619.1"
                     /translation="MKIKEQTRKLAAGCSKYSFEVADKTDEVSSKHCFEVVNRTDAVS
                     R"
     gene            complement(1866..2582)
                     /locus_tag="VVE42_000358"
     CDS             complement(1866..2582)
                     /locus_tag="VVE42_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEU3072620.1"
                     /translation="MKKRYLVLTALLALSLAACSQEKSKNEDGATKTEQTAKAEGTVG
                     SKSQGAAQKKAEVVNKGDYYSIQGKYDEIIVANKHYPLSKDYNPGENPTAKAELVKLI
                     KAMQEAGFPISDHYSGFRSYETQTKLYQDYVNQDGKAAADRYSARPGYSEHQTGLAFD
                     VIGTDGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AASGLTLEEYFGFEGGDYVD"
     gene            complement(2585..3056)
                     /locus_tag="VVE42_000359"
                     /pseudo
     CDS             complement(2585..3056)
                     /locus_tag="VVE42_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
     gene            complement(3058..4095)
                     /locus_tag="VVE42_000360"
     CDS             complement(3058..4095)
                     /locus_tag="VVE42_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEU3072621.1"
                     /translation="MNQTVKYIKELTAIASPTGFTREISNYLVKTLEGFGYQPVRTAK
                     GGVNVTIKGQNDEEHRYVTAHVDTLGAIVRAVKPDGRLKMDRIGGFPWNMIEGENCTV
                     HVASTGQKVSGTILIHQTSCHVYKDAGTAERTQDNMEVRLDAKVSNEKETRALGIEVG
                     DFISFDPRTVVTEAGFIKSRHLDDKVSAAILLNLLLIYKEEKIELPVTTHFAFSVFEE
                     VGHGANSNIPTQVVEYLAVDMGAMGDDQQTDEYTVSICVKDASGPYHYDFRQHLVALA
                     KEQDIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVVATERM
                     VDAYLRSNLVD"
     gene            complement(4193..5518)
                     /gene="brnQ"
                     /locus_tag="VVE42_000361"
     CDS             complement(4193..5518)
                     /gene="brnQ"
                     /locus_tag="VVE42_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEU3072622.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRILTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVVGIVVALAFGALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVMVNKFVALSKPGMQLTIAVVTVIAIASVLGS
                     SFKVEFLANLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            complement(5689..5781)
                     /locus_tag="VVE42_000362"
     CDS             complement(5689..5781)
                     /locus_tag="VVE42_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU3072623.1"
                     /translation="MRSARACQFLLEQGYNVINVQGGMLVFEEL"
     gene            complement(5824..5976)
                     /locus_tag="VVE42_000363"
     CDS             complement(5824..5976)
                     /locus_tag="VVE42_000363"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU3072624.1"
                     /translation="MKEIAFDAFYQLYQNDQLSLVDVREVDEFAALHLECAHNLPLSQ
                     LADSYD"
     gene            complement(6136..6714)
                     /locus_tag="VVE42_000364"
     CDS             complement(6136..6714)
                     /locus_tag="VVE42_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEU3072625.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFAAPKTARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPRT
                     GFAEKWHPQLLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKDYLPAYFPLV
                     HPSPRNQIWMAKNPWFEAEVVPDLKKRIKTIL"
     gene            complement(6768..8168)
                     /gene="pepV"
                     /locus_tag="VVE42_000365"
     CDS             complement(6768..8168)
                     /gene="pepV"
                     /locus_tag="VVE42_000365"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEU3072626.1"
                     /translation="MTAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGP
                     GPVKALKKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENAGVARLHSFTGGLREN
                     MVPESATAVISGDLADLQAKLDVFVTEHKLRGELQEEAGKYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFDFAGPAKEYLDIAGKILLNDHEGENLKIAYVDEKMGALSMNAGV
                     FRFDETSADNTIALNIRYPKGTSPEQIKSILENLPVVSVSLSEHGHTPHYVPMEDPLV
                     QTLLNVYEKQTGLKGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            complement(8181..8786)
                     /locus_tag="VVE42_000366"
     CDS             complement(8181..8786)
                     /locus_tag="VVE42_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEU3072627.1"
                     /translation="MKFLELNKKRHATKKFTDKSVDPKDVRTAIEIATLAPSAHNSQP
                     WKFVVVREKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYSEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIEDRFRPELLITVGYTDEKLEPSYRLPVDEIIEKR"
     gene            8957..9757
                     /locus_tag="VVE42_000367"
     CDS             8957..9757
                     /locus_tag="VVE42_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU3072628.1"
                     /translation="MKKFSLLLAILPLLVACGNQATPKETNSQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAERYLYSLPISNNPLVLVSNKKNPLTSLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
                     NPATIDFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSADSPSN
                     YIIFSSDQKEFKEKFDKALKELYQDGTLEKLSNTYLGGSYLPDQSQLQ"
     gene            complement(9939..10772)
                     /locus_tag="VVE42_000368"
     CDS             complement(9939..10772)
                     /locus_tag="VVE42_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEU3072629.1"
                     /translation="MNHKIAILSDIHGNATALEAVIADAKDQGVSEYWLLGDIFLPGP
                     GANDLVALLKDLPITASVRGNWDDRVLEALDGEYGLEHPQEIQLMRMTQFLMERMDPE
                     TIVWLRSLPLLEKKEVDGLRFSISHNLPNKNYGGDLLVENDTEKFDQLLDAETDVAVY
                     GHVHKQLLRYGSQGQQIINPGTIGMPYFNWEVLKNHRSQYAVIEVEDGELVNILFRKV
                     AYDYEAELKLAKSKGLPFIEMYEELRRDDNYQGHNLELLASLIEKHRYVEDVKNFFDF
                     L"
     gene            complement(10762..12609)
                     /gene="uvrC"
                     /locus_tag="VVE42_000369"
     CDS             complement(10762..12609)
                     /gene="uvrC"
                     /locus_tag="VVE42_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEU3072630.1"
                     /translation="MNNLIKSKLELLPTSPGCYIHKDKNGTIIYVGKAKNLRNRVRSY
                     FRGSHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPFI
                     KITNERYPRLIITRQVKKDGGLYFGPYPDVGAANEIKRLLDRIFPFRKCTNSPSKVCF
                     YYHIGQCMAHTICKKDEVYFKSMAQEVSDFLKGQDNKIIDELKGKMAAAAQTMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDQEAVQALVDTKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGKPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQRDGLTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVELSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMKHFKSLTKIKEA
                     SVDEIVEVGVPRAVAEAVQRKLNPQEEVELAQVAEERVDYQTEGNYHEP"
     gene            complement(12649..13350)
                     /locus_tag="VVE42_000370"
     CDS             complement(12649..13350)
                     /locus_tag="VVE42_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEU3072631.1"
                     /translation="MRWLFRLIGAFFSFVWRLFWRLVWIVVLLCVLAFGLLWYLNGDF
                     QGALKQAERSVKIGQQSIDQWEKTGQLPKLSQTDSHQHSEGRWAQASARIYLDPQMDS
                     RFQEAYLEAIQNWNQTGAFNFELVTESSKADITATEMNDGGTPVAGEAESQTNLLTGQ
                     FLSVTVRLNHYYLSNPYYGYSYERLVHTAEHELGHAIGLDHTDEKSVMQPAGSFYGIQ
                     EEDVANLRKIYETSE"
     gene            complement(13429..14661)
                     /gene="murN"
                     /locus_tag="VVE42_000371"
     CDS             complement(13429..14661)
                     /gene="murN"
                     /locus_tag="VVE42_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HEU3072632.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNPIDAEKKSIIQGLTDLGYQFDGLTTGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEHFYDTFGEQAEFLIASLNFSDYMSKLQGEQSKLEENLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFRYH
                     PSPLKYKAIQLLKKIVGR"
     gene            complement(14666..15886)
                     /gene="murM"
                     /locus_tag="VVE42_000372"
     CDS             complement(14666..15886)
                     /gene="murM"
                     /locus_tag="VVE42_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HEU3072633.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFAIQSIKSYARSKRAV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQAIRTARNKGLEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRTSKVEAQKKE
                     KERLLEELTFLQEYIDVGQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMVWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMSTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            complement(15998..16777)
                     /locus_tag="VVE42_000373"
     CDS             complement(15998..16777)
                     /locus_tag="VVE42_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEU3072634.1"
                     /translation="MAVMKIEYYSQVLDMEWGMNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYTALAEELPQVLK
                     RFFPNMTSKREKTFIAGLSMGGYGCFKLALATNRFSHAASFSGALSFQNFSPESQNLG
                     SPAYWRGVFGEIRDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            complement(16846..18507)
                     /locus_tag="VVE42_000374"
     CDS             complement(16846..18507)
                     /locus_tag="VVE42_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEU3072635.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPERIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKVLSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILRESSELINQT
                     VEDYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            complement(18682..20904)
                     /gene="recJ"
                     /locus_tag="VVE42_000375"
     CDS             complement(18682..20904)
                     /gene="recJ"
                     /locus_tag="VVE42_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEU3072636.1"
                     /translation="MITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQDQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEEGENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSMPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIAFMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPHRDLFIAFGGHAGAAGMTLEVEQLSDLSQVLEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GEASFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMVDARVEGVQLFN
                     IRGKNAVLPEGVPVLDFPGELPNLAASEAIVVKNIPEDITQLKTIFQEQHFSAVYFKN
                     DIDKAYYLTGYGTRDQFAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
     gene            21181..21939
                     /locus_tag="VVE42_000376"
     CDS             21181..21939
                     /locus_tag="VVE42_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU3072637.1"
                     /translation="MALVEFKNVEKYYGDYHALRNINLRFEKGQVVVLLGPSGSGKST
                     LIRTINGLEAVDKGSLLVNGHQVAGASQKDLVPLRKEVGMVFQHFNLYPHKTVLENMT
                     LAPIKVLGIDKKEAEKTAQKYLEFVNMWDKKDSYPAMLSGGQKQRIAIARGLAMHPEL
                     LLFDEPTSALDPETIGDVLAVMQKLAHNGMNMIIVTHEMGFAREVADRIIFMTDGEVL
                     VDTTDVDNFFDNPSKPRAQQFLSKIINHESDKVK"
     gene            21979..22743
                     /locus_tag="VVE42_000377"
     CDS             21979..22743
                     /locus_tag="VVE42_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU3072638.1"
                     /translation="MISLFGLAAAKPVQADTSIADIQKRGEPVVGVKQDVPNFGYKDP
                     KTGTYSGIETDLAKMVADELKVKIRYVPVTAQTRGPLLDNEQVDMDIATFTITDERKK
                     LYNFTSPYYTDASGFLVNKSAKIKKIEDLNGKTIGVAQGSITQCLITELGKKKGLTFK
                     FVELGSYPELITSLHAHRIDAFSVDRSILSGYTSKRTELLDDSFKPSDYGIVTKKSNT
                     ELNDYLDNLVTKWSKDGSLQKLYDRYKLKPSSHTAD"
     gene            22756..23433
                     /locus_tag="VVE42_000378"
     CDS             22756..23433
                     /locus_tag="VVE42_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU3072639.1"
                     /translation="MTDLSSWTAYFQDFGQFFNGFLFTLALAIGSFILAMVLGIFFGA
                     MSTSKRPILRILARIFVEFYQNTPLLVQFVIVFYGLPLISDHTIMIPIYWTAVLCVGL
                     YHGAYIAEVIRSGIQSIPSGQMEAALSQGFTYISAMRLIILPQTFRIILPPLTNQIVN
                     LIKNTSTVAIISGVDLMFVTKSWSALNGNYIPAFLGAALLYFALCFPVAQFGRKMEQA
                     NKKAYSL"
     gene            23443..24102
                     /locus_tag="VVE42_000379"
     CDS             23443..24102
                     /locus_tag="VVE42_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU3072640.1"
                     /translation="MESILEVLTPDNLVFIFKGFGLTLYISLIAIILSTIIGMVLAVM
                     RNGKNPVLRIISSIYIEFVRNVPNLLWIFTIFLVFKMKSTPAGITAFTLFTSAALAEI
                     IRGDLNAVDKGQYEAGMSQGFTSAQILYYIILPQAIRKMLPAIISQFVTVIKDTSLLY
                     SVIALQELFGASQILMGRYFEPEQVFSLYILIALIYFSFNLAISSLSHMLAKRWQQAA
                     E"
     gene            24398..25588
                     /locus_tag="VVE42_000380"
     CDS             24398..25588
                     /locus_tag="VVE42_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEU3072641.1"
                     /translation="MKSIKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSLTFIL
                     ATRLPLLESWFHGLEKVYTVHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNLAIYI
                     FASIILVAYLGKYIQYEAWRWIHRLVYLAYILGLFHIYMIMGNRLLTFNLLSFLVGSY
                     ALLGLLAGFYIIFLYQKISFPYLGKITHLKRLNHDTREIQIHLSRPFNYQSGQFAFLK
                     IFQEGFESAPHPFSISGGHGQTLYFTIKNSGDHTKNIYDNLQAGSKVTLDRAYGHMII
                     EEGRENQVWIAGGIGITPFISYIREHPILDKQVHFYYSFRGDENAVYLDLLRNYAQKN
                     PNFELHLIDSTKDGYLNFEQKEVPEHATVYMCGPISMMKALAKQIKKQNPKTELIYEG
                     FKFK"
     gene            complement(25909..26790)
                     /locus_tag="VVE42_000381"
     CDS             complement(25909..26790)
                     /locus_tag="VVE42_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEU3072642.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSEGKA"
     gene            complement(26931..28259)
                     /gene="vncS"
                     /locus_tag="VVE42_000382"
     CDS             complement(26931..28259)
                     /gene="vncS"
                     /locus_tag="VVE42_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HEU3072643.1"
                     /translation="MKRTGLFAKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGGTMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKWVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKDRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDREIIDLLQMTQNLVKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENRLTFFIDFPKVVQD"
     gene            complement(28256..28912)
                     /gene="vncR"
                     /locus_tag="VVE42_000383"
     CDS             complement(28256..28912)
                     /gene="vncR"
                     /locus_tag="VVE42_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HEU3072644.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASLAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIKELRKKLDLDCILTVRNVGYKLERK
                     "
     gene            complement(28961..29044)
                     /gene="pep27"
                     /locus_tag="VVE42_000384"
     CDS             complement(28961..29044)
                     /gene="pep27"
                     /locus_tag="VVE42_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198523890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HEU3072645.1"
                     /translation="MRKEFHNVLSSDQLLTDKRPARDYNRK"
     gene            complement(29224..30603)
                     /gene="vex3"
                     /locus_tag="VVE42_000385"
     CDS             complement(29224..30603)
                     /gene="vex3"
                     /locus_tag="VVE42_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HEU3072646.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMALASSNLLRKQ
                     PKELLLDGE"
     gene            complement(30655..31302)
                     /gene="vex2"
                     /locus_tag="VVE42_000386"
     CDS             complement(30655..31302)
                     /gene="vex2"
                     /locus_tag="VVE42_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HEU3072647.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQIDYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIR
                     LVNKKASKNTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            complement(31315..32592)
                     /locus_tag="VVE42_000387"
     CDS             complement(31315..32592)
                     /locus_tag="VVE42_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU3072648.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGDSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYESSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKSSIFLQF
                     CLEVVLVSLGALLPAFVAGNGITTYLLQTLLASGDQASLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
BASE COUNT         9258 a         7470 c         6084 g         9850 t
ORIGIN      
        1 gccaaactgg aagcgaccaa taatctcatc aaacttatca agcgcaatgc ctttggtttt
       61 cgaaactttg aaaacttcaa aaaacggatt tttatcgctc tgaatatcaa aaaagaaagg
      121 acaaaatttg tcctttctcg agcttagctg acttcaaccc actacagttg acaaagagcc
      181 aaaaaaagtt gagacatagc gtcttcaact tttttacatt actttaacta ctgattaaag
      241 tgagtttaca tcaactttga taccaacacc ttgagtagtt gtgatagtca agtttgttac
      301 gtaagttcct ttagctgtag ctggttttgc tttttggatt gtttcgttga aagctttaaa
      361 gttttcaacc aatttttcag cttcaaatga tactttaccg atgattgctt gaacgttacc
      421 tgcacggtca gcacggtaag tgattttacc acctttagac tcttcaactg ctttagcaac
      481 atccattgtt acagtaccag ttttagggtt tggcatcaag ttacgtggtc caaggacacg
      541 tccaagacgt ccaacaagag ccatcatatc aggtgtagcg ataactacgt cgaagtccaa
      601 ccaaccgtcg ttgattttag caacaaggtc atcttcacca acaaagtctg caccagcagc
      661 ttttgcttct tcagcttttg caccacgtgc gaaaacaaga acacgtgaag ttttaccagt
      721 accgtttggc aataccattg ctccacggat ttgttggtca gcttttttaa cgtcgatgtt
      781 caagttgtaa gcaacttcta cagttgcgtc aaattttgca aagttagttt cttttgcaag
      841 tgctacagct tcttctacgc tgtatgcttt tgtgctgtcg attttctcaa gagcagcacg
      901 aagttgtttg ctttttttag ccattttcta ttctccttgt aagtggttca atcgattttc
      961 atctcccacg tcacttctcg aaatgatgaa gtcttgcggg ttttcagtct actatttttc
     1021 tcttcctacg gccttaagct cccttgctgt acccaagtac aagcctcagt cacttgccta
     1081 gtatgaaaag caactaatag actgctgtga gattttctac tgtgttattg attagtcaac
     1141 aacagtgaat cccatagaac gagcagtacc ttcgatcata cgcattgcag actctacgtt
     1201 tgctgcgttc aaatctggca tcttagtttc tgcaatttct tgtacttgtg cacgagtaac
     1261 tgtagcaact ttagttttat taggtgtacc tgatcctttt tcaacacctg cagctttttt
     1321 caaaagaaca gcagctggtg gtgttttcgt aacgaaagta aatgatttat cttcgtaaac
     1381 tgagataaca actggaatga tcataccagc ttggtcagct gtacgagcgt tgaactcttt
     1441 tgtgaatccc atgatgttga taccagcttg accaagagca ggtccaaccg gtggagctgg
     1501 tgtagcttta ccagcaggga tttgcaattt tacaagtttt tcgacttttt tagccatttt
     1561 taaatcctcc tttgtggttt tggcggtaat tagagatttt tacctcccac aagtatgttt
     1621 ttcacatacc catccattat acactattta tctggaattg caagagaaaa gtttattttt
     1681 tatactcaat gaaaatcaaa gagcaaacta ggaagctagc cgcaggctgc tcaaagtaca
     1741 gctttgaggt tgcagataaa actgacgaag tcagctcaaa acactgtttt gaggttgtga
     1801 atagaaccga cgcagtcagt agataaggcg acgctgacgt ggtctgaaga gattttcgaa
     1861 gagtattaat cgacgtaatc gccgccttca aatccaaagt attcttccaa agttagacca
     1921 ctcgcagcaa tttcttttgc ttcttttcct acataacgaa ggtgccattc ctcagccata
     1981 tagcctgttt ctttctcctt acctttgaga taacggacaa caaagccata atcagcagca
     2041 tggtccaaaa gccattgagc cgctttttcc tctgttacca aatcaccatc agtcccaatc
     2101 acatcaaagg ccaagcctgt ttggtgttcg ctatagccag gacgggcaga gtaacggtca
     2161 gctgctgcct tcccatcttg gttgacataa tcttgataga gcttggtctg agtttcataa
     2221 cttctaaaac cactataatg gtcgctaatc gggaaacctg cctcttgcat cgctttgatg
     2281 agtttgacca actctgcctt ggctgttgga ttttcccctg gattgtagtc ttttgacaaa
     2341 ggataatgtt tatttgctac gatgatttca tcgtattttc cttggatgct gtagtagtct
     2401 cctttattga ccacttctgc ttttttctgg gcagctcctt gagacttact tccgactgtt
     2461 ccttcagctt tagctgtttg ttctgtctta gttgcgccat cttcattttt ggatttttct
     2521 tgtgaacacg ctgctagact caaggctagc aaggctgtca agacaaggta tctttttttc
     2581 atttctactc ctttatttct aatagtttat ctacttctga cgataaacgt ttgactaatt
     2641 tttcaatctc atcaggtttt gcttggaagg tttctgaggt catttcttca aaggaaaccc
     2701 accagactgg accacgtcca ttgagaccat gaccattcat gtcacctgtt cgtctaggga
     2761 aaaggtgcca atgaagatgg gcatcgccat ttcctagcag ttcgatattc attttctcag
     2821 cagcaaaggc cttggcaact gcctcttgga ctaaactcat ttcttctaga aaatggagtc
     2881 ttgtgtcctt ttctaaaatg gtgcaattcc gtgacatgtt ccttggctaa aaagagacta
     2941 tagcttgcaa aatactgatg gtctccaatc acaagatagc ctgtttccaa ctctttgaca
     3001 aagtagggat tttctccctt cttgatgagc tcaattctct ggcaaataag gcacatatca
     3061 atctactaaa ttgcttctaa gataggcatc gaccattcgt tctgttgcga ccaccgagtc
     3121 aatatgagtg cgctcataag aatgactaga ctcgataccc gcaccaagaa gggcatgctt
     3181 gacctctgct cctgctgaca tagctgctga agcatccgaa ccgtaaaatg gataaatgtc
     3241 gagcttaaat ggaatatctt gctcctttgc caaagccact aaatgttgac ggaagtcata
     3301 gtgataggga cctgaagcgt ccttgacaca gatagacact gtatactcat ccgtctgctg
     3361 gtcatctccc attgccccca tatcgacagc tagatattcc accacttgag ttggaatatt
     3421 ggaattagca ccgtggccca cctcttcgaa aactgaaaaa gcaaaatggg ttgttactgg
     3481 caattcaatc ttctcttctt tataaataag aagcagatta agcaaaattg ctgcactgac
     3541 cttatcgtcc aaatgacgag acttgataaa acctgcctct gtcacgacag ttcgtggatc
     3601 aaagctgata aaatcaccga cctcaatccc caaggcacga gtttcttttt cattgcttac
     3661 ttttgcgtcc aaacgtactt ccatattatc ctgcgtgcgt tctgcagttc ctgcatcctt
     3721 gtagacatgg caagaagttt ggtggatgag gatggtacct gatacctttt gacctgtact
     3781 agctacgtga acggtacagt tttctccttc aatcatattc caaggaaaac caccgatacg
     3841 gtccattttg agacggccat ctggtttgac agcacgaaca atggcaccta acgtatccac
     3901 atgggcagtc acatagcgat gctcttcatc attttgacct ttgatggtca cattgacacc
     3961 acccttggct gtgcgaaccg gctggtaacc aaaaccttct agagtcttga ctaaataatt
     4021 cgaaatttca cgagtgaagc ccgttggcga tgcgatggct gtcagttctt tgatatattt
     4081 tactgtttga ttcatttctt cacctctttc gctacctatt ataacacatt tatcatcgga
     4141 taaaaaaaga aaatcactcc tgtagacttg gctacaaaag tgatttaagt gattattcca
     4201 tttcaaaaac atcgctttct tgcttgtttg gtagaaccaa tgagagcaag attccaacaa
     4261 tggctggcac caaccatggg agagatgcct tggcaaaagg aagaacgcta acaagatttg
     4321 caagaaactc aaccttaaac gagcttccta gtacgcttgc aatggcaata actgtaacca
     4381 cagcaattgt caactgcata cctggttttg aaagggcaac aaatttgttg accatgacaa
     4441 tcataacgat agcaatcgtg attgggtaca agataaccag tactggaatt gagtacttga
     4501 taatcgcatc aagacccaaa ttggcaatag caaatccaat caaggtaaag gctgtcgcat
     4561 aaaccttgta gctgatttgt gggaagcgct cattaaagaa ctcagctgtt gacacaatta
     4621 aaccaacagt cgttgtgaag caggttacgg taaccatagc tgcaaggaag agttgagctg
     4681 ttgagccaaa gatttcttga gtggcttgtg acaagatgta aacacctggt gttccaccct
     4741 tcatcgcttc agctggtact gggaaatgat ttccaagaaa acctaaaccg atgtaaagag
     4801 cgccgaaggc aagggcaaca acgataccaa caacccaaat agttgaaatg tattctttct
     4861 tacttgaaaa tccaagttgt ttcaaggttt gaactgcgat tacgctaaag gccactgagg
     4921 caagggcgtc caaggtattg taaccttcta ggaaacctgt accaaaggca gaagcttgat
     4981 aagcagctga agcagcttga ggacttgttc caccatattt gatagctccc agaacgacca
     5041 agataacaat caaaattgca aagactggcg ttaaaatacg tccaatgcgg tctaagattt
     5101 ttgatggatt aagcgaaatc aaataggctg ccgcaaaata cagaaccgta aatacaatca
     5161 agccaagtcc tttatttgca tccgacaaaa gggggctaat ccctacttcg taagctgttg
     5221 tagcagtacg tggggtagca aagaatggac cgattgacaa gtaaagaact gagaggtaaa
     5281 gggtcgcaaa ccaaggcgct atcttcgttg aaatctcgta gatatatcct ttaggattta
     5341 gcgttccaat aataagggtc aagacggcga taccaacgcc tgaaaagaca aaacctgcga
     5401 tggcaggaag aaaatgttct ccagatagag cacctagaga aggcggaaaa atcaagttcc
     5461 ccgcaccaaa aaatattcca aacaggagca aacctgttag ggtacctttt ttagccataa
     5521 aaatctcctt catatttata aaatactgac ctagtatacc acgattagga gtctgaaaaa
     5581 agtatcacga ggcaaatatt aaatgttttc aattttctta aaaataagaa ttatctaaaa
     5641 ataaaactct cctacccagt ccacaccaag taggagaaat gcaaaatttt aaagttcttc
     5701 aaagactaac atgccaccct ggacattgat aacattataa ccttgttcta acaggaattg
     5761 gcaagcacgc gccgatctca ttccagattt gcaaataata taatgcaagc gatctttgtc
     5821 caattaatca taactatcag ccaattgact aagcggtagg ttgtgggcac attctaaatg
     5881 aagagctgca aactcatcca cttctctcac atccactaga gaaagctggt cgttttggta
     5941 aagctggtaa aatgcgtcaa aggctatttc tttcattctt tttcttctaa tactcttcga
     6001 aaatctcttc aaaccacgtc agcgtcgcct taccgtgcgt atgttaccga cttcgtcagt
     6061 tttatctgca acctcaaagc tgtactttga gcagcctacg actagcttcc tagtttgctc
     6121 tttgattttc attgactata aaatggtttt aattcttttt ttcaaatctg gcactacttc
     6181 tgcctcaaac caaggatttt tggccatcca gatttgattt cgtggtgatg ggtgaactag
     6241 cggaaaatag gctggcagat agtctttata gtgtttcacc ctctccgtta ccttcccact
     6301 gattttctcc tgtaaatagt aggcttgggc atattgccca atcaagaggg ttaactgaat
     6361 atcaggcaat tcctgtaaga gctgcggatg ccatttttct gcaaaacctg tacgaggcgg
     6421 aagatcaccc gacttgccat gtcctggaaa gtagaaatcc ataggcaaaa cagcaaaata
     6481 acctgaattg taaaaggtat cttcatccac acctagccag tcccgcaagc ggtcaccact
     6541 tttatctttc cagtaaaggc ctgcttcttg agttttaagt cccggagcct gaccgatgat
     6601 attgatgcga gcagtttttg gcgctgcaaa gagaggctca atgccacgct ctgtatagct
     6661 ggcattctgt gaatccgcca tgatagcctg tttgattctt tcgatttgag acatctactt
     6721 tccctccaaa aagaagtcca agcataagat ctcagacttc tatcgtttta tttgatcaat
     6781 tcgtaaatag cttcggcata gattgctgct gctcggaaga gatcgtccaa ggcgataaat
     6841 tcattggctt ggtgcatggt atcaattgag tctgggaaca tagcaccata ggcaactccg
     6901 cgctctaaca agcgaccaaa ggttccacca ccgatgactt gttcatgacc tttaaggcct
     6961 gtttgttttt catagacatt caacaaggtt tgcacaagtg gatcttccat tggcacatag
     7021 tgaggcgtgt gaccgtgttc agacaggcta acagaaacaa ctggcaagtt ttcaaggatt
     7081 gacttgattt gttctggact tgttcctttt ggatagcgga tgttgagggc aatggtatta
     7141 tcagcgcttg tttcatcaaa gcggaagacg cctgcattca tagaaagggc acccatcttt
     7201 tcatccacat aagcgatctt gagattttca ccctcatggt cgttcaagag aattttacca
     7261 gcgatgtcaa ggtattcttt ggctggacca gcaaagtcaa actggctaag gaagagggct
     7321 aggtaagtcg caccattgac acctgaagca ggcatagcac cgtgggctga tttaccaata
     7381 atcgtcacct tgtatttgcc agcttcttct tggagctctc ctctaagttt gtgttctgta
     7441 acgaagacat ctagtttagc ttgcaagtca gccaagtcac ctgaaataac tgctgttgct
     7501 gattcaggta ccatgttttc acgcaaacca cctgtaaagc tatgaagacg ggcaacacct
     7561 gcattttcac ctgcaaagtg gaggtattcc gtgatatttc ctttttcgcc gttgatgata
     7621 gggaattcag cgtctggaga gaaaccaaag tctggcttgg caagtcctac gtgctcgaag
     7681 tagtagtcca tatctgccca gcctgattct tcgtctgttc cgacgatgaa acgaactttc
     7741 ttagaagttg gaagacccaa ttctttgatg attttcaaac catagtaaca agctgttgta
     7801 ggacccttat cgtccgaagc tccgcgcgca taaaggcgac catctttgat agttggtgtg
     7861 taaggatctg tatcccaacc gctaccagct ggaaccacgt ccatgtgggc aaatattccg
     7921 agaacttctt ctccatcacc aaactcaaaa tgtcctgcat agttatcaac atttttagtt
     7981 ggataaccat cacggtctgc gatttcaagg aattttttca aggcttttac tggaccaggt
     8041 ccaaatggat gctgggcatc agccttgctg tcatcacgtt ctgagttaat ttccaaaagg
     8101 ctaaacaagt cagccaagag gtcttctttg cgtttttcta cttctgctgt aaaatcaatt
     8161 gctgtcattt tttcttcttt ctatcttttc tcgatgattt catctactgg caagcggtag
     8221 cttggttcca atttttcgtc tgtataaccc actgtgatca agagttctgg gcggaaacgg
     8281 tcttcgattt ccaaaacttc attaactttt gatttgtcaa aaccaaggat aatattggag
     8341 ccaattcctt ggtctgtaag agcgagaacc aagttcatag caaccaaacc tgcattgagt
     8401 gctaggtagt cgctgacttg ttgctcactg taacgggcaa actcagctgg cagatttttc
     8461 atgaagtatt gaagttgttc ttctgagaaa ttgttagcac caccaactcg ggcaatctta
     8521 cgagcgcgtt tggctaagtc cgtatctgta aacaaggcaa tggttacagg cgctgatgat
     8581 acctgttcaa aattggaacc ataagctaac tttgccagtt cagcattttt ctcacgaacc
     8641 accacaaact tccatggctg gctgttgtgg gcacttggag ccaaagtcgc gatttcgata
     8701 gcagtacgca catcttttgg atcaactgac ttatcagtga acttcttagt cgcatgacgt
     8761 tttttgttta attcaagaaa tttcataatc gatttccttt tctaattcta ctgcttccat
     8821 tctaccatat tttgaaagag cttgcagaga tttttcatga ttaagatttt ttattacagc
     8881 cataaaaaat atatattagt tcatcgggaa agtttctgat aaactagcaa gtactttaca
     8941 tttgaatgga gacagtatga aaaaatttag cctattacta gccatcctcc cacttttagt
     9001 tgcctgtgga aatcaagcca cacctaaaga gactaactct caaaagacaa tcgtccttgc
     9061 tacagctggc gacgtgccac catttgacta cgaagataaa ggcaatctga caggcttcga
     9121 tatcgaagtt ctaaaggcag tagatgaaaa actcagcgac tacgagattc aatttcaaag
     9181 aactgcttgg gagagcatct tcccaggact tgattctggt cactatcagg ctgcggccaa
     9241 taacttgagt tacacaaaag agcgcgctga aagatacctc tactcacttc cgatttccaa
     9301 caatcccctc gtcctcgtca gcaacaagaa aaatcctttg acttctcttg accagatcgc
     9361 tggtaaaaca acacaagagg ataccggaac ttctaacgct caattcatca ataactggaa
     9421 tcagaaacac actgataatc ccgctactat tgatttttct ggtgaggata ttggtaaacg
     9481 aatcctagac cttgctaacg gagagtttga tttcctagtt tttgacaagg tatccgttca
     9541 aaagattatc aaggaccgtg gtttagacct ctcagtcgtt gatttacctt ctgcagatag
     9601 ccccagcaat tatatcattt tctcaagcga ccaaaaagag tttaaagaga aatttgataa
     9661 agcgctcaaa gaactctatc aagacggaac ccttgaaaaa ctcagcaata cctatctagg
     9721 tggttcttac ctcccagatc aatctcagtt acaataatac tcttcgaaaa tctcttcaaa
     9781 ccacgtcagc tttatctgca acctcaaagc tgtgctttga gcaacctacg gctagcttcc
     9841 tagtttgctc tttaattttc attgagtata agatatatta aaagcgatgg gatcagccaa
     9901 tcgctttttt agtttgcatt ggctatttta ggaaactctt acaaaaaatc aaaaaaattc
     9961 ttcacatcct ctacatacct atgcttttct attaagcttg ctaataattc cagattgtgc
    10021 ccctgatagt tatcgtcacg acgcagttct tcatacattt cgataaaggg aagccccttg
    10081 gacttggcca atttcaactc cgcttcgtag tcataagcga ctttacgaaa taggatattt
    10141 accaattccc catcttcaac ctctatcacg gcatactggg aacggtgatt ttttaacacc
    10201 tcccaattaa aatagggcat accaatcgta ccgggattga tgatttgttg cccttgacta
    10261 ccataacgaa gcaactgctt gtgaacatga ccatagacgg ccacgtccgt ttccgcatct
    10321 agcagttggt caaatttctc tgtatcattc tcaactagca agtcaccacc atagttctta
    10381 tttggcaaat tatgagagat agaaaagcgt agcccgtcaa cttctttctt ttccagcaaa
    10441 ggcaagcttc gtagccagac aatcgtttca ggatccattc gctccatcaa aaactgggtc
    10501 attcgcatga gctggatttc ttgcggatgt tccaaaccat attcgccatc caaggcctca
    10561 aggacacgat catcccaatt gcctcgaaca cttgctgtga taggaaggtc ctttagcagg
    10621 gcgaccaagt catttgcacc tggaccggga agaaaaatat ctcccagaag ccagtattca
    10681 ctgacccctt gatctttagc atctgcaatc actgcttcta gcgccgtcgc attgccatga
    10741 atatctgata aaattgcgat tttatggttc atgatagttt ccttccgttt ggtaatctac
    10801 tctctcttcc gccacttgag ccaattctac ttcttcctga gggttcaact ttctctgaac
    10861 agcttctgct actgctctag gcacaccaac ttcgacaatt tcatccacac tggcttcctt
    10921 gattttagtt agagacttga aatgcttcat gagattctgc ttgcgtttag gtcccagacc
    10981 gtcaattcca tccagttgtg atgaaaaaga atttttggag cgcagttggc ggtgaaaggt
    11041 gatggcaaag cggtggacct catcttggat gcgttggagg aggaaaaatt cctgagaatt
    11101 gcgtgacaac tccactacct caagcggatc tccaaagagc aattcatggg tttggtgctt
    11161 gtcattcttt tgcagacctg caatggggat atccaagccc agttcctctt ggatgacttg
    11221 cttagcgata ttgacttgac cttgtccccc atcaatcaca atcaaatctg ggggagtcaa
    11281 accgtctcgt tgaactctac catagcgtct gcgaatgacc tctcgcatac tggcgtagtc
    11341 atctggacct acaaccgttt ttatcttgta cttacggtaa tccttcttac tcggtttacc
    11401 attgacaaag accaccatgg ctgaaacagg gctagttccc atgatgttgg agttgtcaaa
    11461 agactcaatg cgaactggag ttggaatttg tagaagtcgt cctaggtttt caatagcccc
    11521 ttgggtcttt tcgactgatt tttctagcag gttaaatttc tgctccagac taacacgggc
    11581 atttttgatg gctagattga ccagctgttt tttctctcca cgctggggtt tgagaatctt
    11641 agtatccacc aaggcctgaa cggcttcttg atcaatatcc tgcggaatca gtacctcatt
    11701 gggaactagg tgagattttt cttgatagaa ttgtcccaca taggtcaaga aatcctcatc
    11761 tggatcattg aagtagggga agagattgac atcgcgctcg atgagcttgc cctgacggac
    11821 aaagaaaacc tgcacacaca tccagccctt atccacatag tagccaaaga catcgcgatt
    11881 ttggagatcc ttagccatga cccgttgctt ggttcgaagc gttccaatag cctgaatcag
    11941 gtcacggtat tccgccgcac gttcaaactc catagtctgg gctgccgctg ccattttgcc
    12001 ctttagctca tcaatgattt tgttatcctg ccctttcaga aaatcagaaa cctcctgggc
    12061 catagacttg aaataaacct catccttctt acagatggtg tgggccatac attggccgat
    12121 atggtagtaa aaacagacct tagagggcga gttggtacat tttcgaaaag ggaaaatcct
    12181 atctagaagt cgcttgattt cattggctgc ccccacatct ggataagggc caaaataaag
    12241 accaccgtcc tttttgacct gacgagtaat gataagcctt ggatagcgct cattggtgat
    12301 tttgatgaaa ggataggact tgtcatcctt gagcatgata ttgtacttgg gcttgttttc
    12361 cttgatcagg ttgatttcta ggagaagtgc ctcaatatta gactccgtaa caataaattc
    12421 aaaatccaca atttcagaca ccagagcctc tgtcttggta tcatgacttc cacgaaaata
    12481 ggaccgtact cgattacgca gatttttagc ctttcctaca tagataatgg tgccattttt
    12541 atccttatga atgtagcaac cagggctggt cggcaagagc tctagttttg atttaatcaa
    12601 gttgttcata gttccattat agcaaaaaaa gtagggaaag atagtaccct actcactagt
    12661 ctcatatatt tttcggaggt ttgcaacatc ctcttcctgg ataccataaa aggaacctgc
    12721 tggttgcatg acagacttct catctgtatg gtccaagcca atcgcatgac ctaactcatg
    12781 ttctgccgta tggacaaggc gttcatagga gtagccatag tatggattgg acaaataata
    12841 atgattcaac cgcaccgtta cggacaagaa ttgccctgtt aagagattag tttgactttc
    12901 cgcctctcct gccacaggag tgcctccgtc gttcatctcc gtagccgtaa tatccgcctt
    12961 actagactca gtcacgagtt caaagttaaa agcaccagtt tgattccagt tctggattgc
    13021 ttctaaataa gcctcttgaa agcgtgaatc catctgcgga tccaggtaaa tacgagcaga
    13081 ggcctgtgcc caccttcctt cagaatgctg gtgactatct gtctggctta acttaggcag
    13141 ttgccctgtt ttctcccatt ggtcaatact ttgttgacca atttttactg accgttctgc
    13201 ttgctttagc gctccttgaa aatccccgtt cagataccag agaagtccga aagcaagcac
    13261 acataagagc acaactatcc aaaccagacg ccaaaacaaa cgccacacaa aagaaaagaa
    13321 agcccctatc aaacgaaaaa gccagcgcat gtctctccac ctttctagca aataagaatt
    13381 attttactaa aagaagctaa aagaaatcta aatactgact ttttcatctt aacgtcctac
    13441 tatttttttg agtaactgga tagctttgta ttttaaaggc gatggatggt aacggaaagt
    13501 acccgctttg cgtacaatat agccattaaa attctgttta aaacgcaaaa caccatcact
    13561 tccatcaaaa atcccttgaa tgcctaggaa attgtattta ggtattccac gttttatgct
    13621 ttccaacata acatattttt gaagcagtgc aggggcatag aacttattaa actcagtgta
    13681 ggaaccacta aagagataag tcgtttcctg aggcatataa acaaataaac tcccagctaa
    13741 aacaatatct tcttctccat atttttcaat caagtctcgc gcttctgctt ttcgaacttc
    13801 aaacgtttca aattgactag aatattctct cagttgattt tgttttttct cagaatgagg
    13861 atttttactc aaatcaagtc gcaacttgtc caagttttct tctagtttac tttgttcacc
    13921 ttgcaatttg ctcatatagt ccgaaaaatt taagcttgct atgagaaact ccgcttgttc
    13981 tccaaaagta tcataaaaat gctcataata ttctaaactt ttatcactat attctctacg
    14041 ttcagaggtt tcttttgtta tattcttaaa aatcgatagt tcttcacgtt ttaacttttt
    14101 caaccgaatg ccaaaggttt cagccttttt caccaagggt ttaccctttt tgctaaaact
    14161 tttaagcaaa ctcttttcag ttaattcagt taaatcttta tagtataacc aatctggttc
    14221 tccacctggg taacctgttg ttaagccatc aaattgataa cctaaatcag tcaaaccttg
    14281 aataatactt tttttctcag catctattgg attaccttgg ctatcaaaag tttgataagt
    14341 ttcataaggt tttacaagca actctaatac accattttgc ttggcatatt cttttaactc
    14401 tgcataaaaa actggaagag catcttgttg ggtataaatc ggccccgaat tgagttccat
    14461 atgcagacca cccagcatgg gcaagctata aaccagagct gcaacttgaa tttctccttc
    14521 ttgtttcaaa gcaagataaa caattcgagc ccctcttttt tctagcaaat cccccatctg
    14581 gacagattgc ataaaggaac gagaagaaac ctgatcagaa taagtctgaa actcttcttt
    14641 cgtgagtgtt gttagtgcca tatacttact ttctatgttt ttttcttaat gttttacgga
    14701 aatcaagagc aagtcttaac agaggataga gaggatgagt ggacattgta aattcaccca
    14761 agtattcttc aatcgttgga ttaaattttt ccttaaaatg ataaagtcca ccattgagag
    14821 agttttcaac accacctaaa ttttgccaga ccatacctcg ctcaaaggca tagcgagccg
    14881 tttcatacca tgttaaaatt ggtgcattgt aacgtttaaa atcatcatcc ataccagcat
    14941 atatattgac agaggtagta ccaaattcca aactcaaagt agccgctaaa ggaactctcg
    15001 cttgacctac atctatatat tcctgcaaga aggtcaattc ctctaacaaa cgttcttttt
    15061 ccttcttctg cgcttctact tttgaagttc gagtcgactc agtaaaggtc tcttccaagg
    15121 ctctattttt cgctaactgt tcttctaatt cttgcgaacg tttagaaaca tccaaggtgg
    15181 ccaaggtgat ataggccttg tccttaaaat tatctaacaa ttttttataa taggcttcat
    15241 tcctcaaatg aatctctttt cgcttctcag tttttttcat caactccgaa aatgaatcta
    15301 atagttccag tccaccatat tgaatctcaa gccctttgtt tcgtgctgtt cgaatagcct
    15361 gttttgttga cttggaaagt ttatcttctt caaaattttc cttgtatatt ttcgcctgaa
    15421 tacgaggttg aatggtgtct cccatttcct ccgtttttcc tgaccacctt actcccattt
    15481 gttgcaaact atcaataata gccagatttt caggaaattc tgtcttttcc tgattgatta
    15541 aactttgaga taggcaaata cttgggtcaa aagtcacaaa aaccgctctc ttactgcgag
    15601 cataggactt aatagactga atggcaaaat tcaagagttc tttatcccca taatccaata
    15661 taggtcctct tgggatgtaa aacattttat agcctagcgg aagagttcta atcaaaatac
    15721 tagctgtcgc cagtaatttt tcttccctgt aaacaccaaa cttctcatgt tgccaattag
    15781 acttaacttc ctcccaagca ctactttgta atacattggc taattcatgt tctttgacaa
    15841 actgatcata ttctaatgtg ggaatgccaa tttgataacg gtacatttcc tactctcttt
    15901 cctccagtat tttattacta ttctactact ttcgtttgaa aagtctagtg aaaacatatt
    15961 ttattttagt tctactcccc ctatgctgaa gttcaaacta agtcagtctc tcttctaatt
    16021 tgaaatcaat tggtagggtt gttaaaaaaa cttccaattg tttttcccag tagtaccact
    16081 cgtgagttcc agcgctatgg ctataggtca catcaaaacc tagttttttg agatttttca
    16141 ctgcgagatt attggcttcg tacaagaaat cctgttcgcc acaccacgcc caaagtttgg
    16201 tctttttatc cgatttttta gccagacttt caagagaata gggactagtt gtccagtctc
    16261 taatctctcc aaaaacacct ctccagtagg ctggacttcc cagattttga ctttcaggag
    16321 aaaagttttg aaagctgagg gcacctgaaa aactagctgc atgagaaaaa cgatttgtcg
    16381 caagagccag tttgaaacag ccgtagcctc ccatagaaag accagctata aaggtctttt
    16441 cacgcttgct agtcatatta gggaagaagc gtttcagaac ctgtggcaat tcctctgcta
    16501 gagccgtgta gtagtcaaaa ccatactggg tatcggtgta ccaaccattg ctggtattgg
    16561 gcataacaac gatgagatta gttcctcgaa gcaagcgttc tacattggtc cgcttaagcc
    16621 aactattatg atttccagac atcccgtgca aaaggtacaa gacgggaata tcttcacact
    16681 ctggttcttc cactcgattg gcatcagggt agaggacatt catcccccac tccatatcca
    16741 atacttgtga gtaatactcg attttcatta ctgccatgat tttctccttc tatttttcta
    16801 taagaaaaag ccgaaactcg actttctctc tgtttatttc aacgattatt ttgcttccat
    16861 gactactggt aaaatagctg gacgacgctt ggtttggtca aagagatact tggtcaaatt
    16921 atctcgaacc ttccctttaa gatctgccca gtcaaagtca tctccttgaa gataatcttc
    16981 taccgtttgg ttaatcaatt ctgaactttc acggagaata tcgcgactct tcttgagata
    17041 aacaaatcca cgcgtgtgaa cacgagctct agccacaatt ttcttctcac gacggttgac
    17101 tgtaatagcc acgatgaaaa ttccatcctc tgacaagacc ttacggtcac gaagaacaac
    17161 atttccaaca tcaccaatgg cattcccatc aattaagata tctcctgctg aaaccgatcc
    17221 agctggaaca aagtctccat tctcgtaagc catggtcgtc ccctttttag gaatgaagat
    17281 gcgttctggc aacatcccaa ctgccatggc agccttagcg tgagcatcca actcacgata
    17341 ctccccttgg acagggaaga ggtacttagg ttgcaaaaga ttgatcatca gctgcaaatc
    17401 acgcacattt ccgtgccctg atacatgtaa actttgggta atcaatttga caaccccacc
    17461 tgcctgataa atcatatttt ctacacgcgc aacaaaggct tctttagcaa tagacggagc
    17521 cgtagcaata tagaccaggt ccccatcctt gatttctaca taacgatggc gaccaatcga
    17581 catcttacga agtccattga taggttcacc catacgacct gtctcaagaa taatcaactc
    17641 atggtcttca aagcgagaca tatctttagg cttaatcaag agaatttcgt tggctaaaga
    17701 caacttctta agacgaatcg ctgtgcggac gatattttca atatcaaatc ctgtcaagac
    17761 gatacgtcga cctgttttat ccgcagcgtc aaaaatctgc tgaatacgag aaagattact
    17821 ggaaacagct gcaacgatga tacgaccttc ccagtcagca atagtttggg taatttcatc
    17881 cctaacttca ctttcactag ccacctgaat attgctgtct gcattggccg aatcactgag
    17941 gagagccagg acgccgtcac gaccaatctc tgccaaacga gcaaaatcag ttgcataaga
    18001 ttcactagcc gtttggtcaa atttgaagtc acctgtataa acgatgcttc cttccgatgt
    18061 cttcaagaca attcccagac tctctggaac ggagtaagtc gtagggaaga aggaaaccac
    18121 tgtcccacca aaatcaatct ccgtattctc atcaatgaca tggaaatcat taaatttctt
    18181 aacggcatca tttcctttga caaagagctt tgccaactca atggtcaact cagacccaaa
    18241 tacaggaact ttagcctctg ccaagagata aggtagagca ccaatcgcat ccgcatgccc
    18301 gtgggtcaag aaaaccccag caatacggtc gctattttca aaaaggtaat ccatgtttgg
    18361 aatcaccaca tcgaccccta attgttcatt ttcaggatat tttaacccta cattcaaaac
    18421 aaaaatggac tctccaattt cagcaatgta catatttttt ccattctcac gcacaccacc
    18481 aagtgttgtt aaactgatat tactcatttt tcctccaaaa tcttagttta tcttgattat
    18541 agggcaactc taccctctta ttctaaaagt tctaatactt tcagccgaat cttttcctac
    18601 cattatacca tttttttgat gattttcaaa atgaaggtct gttttcaaaa aagagttgat
    18661 ttcccaactc tttcctaact tctactcttt ttccatcaaa aaatcataaa tttcttgcac
    18721 cgtacccagc gccatcattt cttggtcttt aacggtttgt ttgagatttt ggtaaatttg
    18781 actttctcct atctcccgct ttggcgcctc tttattgact gtcatcacac catcttttat
    18841 cgtcacaaag cctagttctt caaatacttg aatcatcttg accagcaaga tttgttgaat
    18901 attaagatat gcagccaaat ctttcagctt gtagcgaata tcaaactctg ggaactggta
    18961 aatagtcttg tacaatttgg caaactgatc tctagtccca taacctgtca gataataggc
    19021 cttgtcaata tcatttttga aatagacagc agagaaatgc tgttcctgaa aaatggtctt
    19081 cagctgagta atatcctctg gaatgttttt tacgacaata gcttcactag ccgcaagatt
    19141 tggcagttct ccaggaaaat ccaagactgg aacaccttct ggcaagactg cattttttcc
    19201 acgaatgtta aaaagttgaa caccttccac tcgcgcatcc accatcatca actggagggc
    19261 agtttggcca ttccattggt tgacagacaa tttaaccgct aactctagat tcttggtttg
    19321 agaaaactct gtcgcccatc tgccttgacc aaaggctacc acttcaaaac tcgcctcacc
    19381 cttggaaatt ttcagcttta gatgggcatt acctgccccc atagtacgag cactttcgac
    19441 ctgaaaattc ttgatataaa aaataggttt ctgattatcc attccaaaag gagctaaacg
    19501 ttcaaaactt ttgaccgttt ccaagctaag tgcctccaaa tccaactctt catctaggtt
    19561 taacttattc ttgccaccag catctgcacc tttttcacga acataatctt ccaaaacctg
    19621 agataaatct gagagttgct caacttccag cgtcataccc gctgcacctg catgacctcc
    19681 aaaggcgata aagaggtctc gatggggatc cagagcttca aaaatatcga ccgcttccac
    19741 actacgagca ctgcccttgg cacgaccgtc ttctatatta agaacaatga ctgtctgtcc
    19801 caattcttcc aataaacgac cagccacgat tcctagaacc ccaggattcc agccttcctt
    19861 ggccaagacc tgaaccttct tctcaggatc caccatggtc ttggcttctt catagataga
    19921 ctgaacgatt tccttgcgct cttcgttttt ctggtgaatc ataaaggcaa tctcatgcgc
    19981 ttcctcatca tcaaatccag tcaacaaatc aatggcagga ttgggatcat ccaagcgacc
    20041 caaggcattc aaacgaggag caatctggaa accaaccgtt tcttctgtta cttcgttggc
    20101 agcaatccca gccatgtcca gcatttcttg cagaccaatg cgctgggtat gacccaacat
    20161 ttccagacca tattgaacta agatacgatt ttcatccgtc agactcacca tatctgcaat
    20221 agttccaata gcgaccaaat caagcaattc cacttgcact tcttctaaca gggcacaagc
    20281 caacttgaaa gcaactccac aaccagccaa atatttaaaa ggataatccg catctggatg
    20341 ttcaggatgg acaatagcat aagcatctgg cagggtttca ggcatggaat gatggtctgt
    20401 cacaatgaca tctactccca tagactgagc caatgcaata gcctcatgac cagcaacccc
    20461 attgtccacc gtcacaatca aggaaattcc ttcttgctcg ataaagtatt tataaacact
    20521 agcattaggg ccatagccat cggtaaaacg atttggcagg taaactcggc actcagcacc
    20581 aagctgttcc aaactttcct tcacaataga agccgaagtc atgccatccg catcatagtc
    20641 tccataaacg agaatatttt ccccttcttc aatagcctga cgaatccgct ccactgcctt
    20701 gtccatatca tggagcagat aagcatcatg taagtcctcc aaggaaggtt ctaaaaactt
    20761 cttcagactt tcttggtcct gaatccctct ctcaaacaat aaccgagcca cctcaggacc
    20821 cagtccagcc ttcttggcta tctttgtaaa atccgcatct tctacctgca gggcaaactg
    20881 ccattcataa gtaggtgtta tcaaaaagac atccactctt tctaagaatt ctattgcttc
    20941 attataacat gatttagacg ttttctctat ccagattgct tttttataac attttatagt
    21001 atattgaaac tagaatagta cacctctact tctaaaatat tgttagaaat cgatttgact
    21061 gtcccgatcg atttgtcctg ttcttatttc actatagtga attttttcag atatggtttg
    21121 acaagaacat tcttttattg tagaatcgtg ttataaaatc taacacaaag gagatctctc
    21181 atggctttag tagaatttaa aaacgtcgaa aaatattacg gagactacca cgcactccgc
    21241 aacatcaatc tccgttttga aaaaggacaa gttgttgtcc tgcttggacc ttctggctct
    21301 gggaagtcca ctcttatccg tacgatcaat ggtttagagg ctgttgacaa aggaagtctc
    21361 ctagtcaatg ggcaccaagt tgctggtgcc agccagaaag atttggtacc tcttcgcaag
    21421 gaagtcggca tggtttttca acattttaac ctttatccac acaaaacggt gttagaaaac
    21481 atgacacttg cacccattaa agttctagga attgataaaa aagaagctga aaaaaccgcc
    21541 caaaaatatc tggaatttgt aaatatgtgg gacaagaaag attcctatcc cgccatgcta
    21601 tctggtggac aaaaacagcg gatcgccatc gctcgtggtc ttgctatgca tccggaactc
    21661 ctcctctttg atgaaccaac atctgctctt gatcctgaga ctatcggaga tgttctagca
    21721 gttatgcaga aactggcgca taatgggatg aacatgatca tcgttaccca cgaaatgggc
    21781 tttgctcgag aggttgcgga ccgcattatc tttatgaccg acggagaagt tttagtagat
    21841 acgacagatg tcgataactt ttttgacaat ccaagcaaac ctcgtgccca acaattcctc
    21901 agcaaaatta tcaaccacga aagtgacaaa gtcaaataag gaggcgccta tgaaaaaaaa
    21961 ttctttttat cagcattatt gattagcctt ttcggccttg ctgctgccaa accagtccag
    22021 gctgatacta gtatcgcaga cattcaaaaa agaggcgaac cggttgtcgg tgtcaaacaa
    22081 gacgttccca attttggtta caaagatccc aagaccggta cttattctgg tatcgaaacc
    22141 gacttggcca agatggtagc tgatgaactc aaggtcaaga ttcgctatgt gccggttaca
    22201 gcacaaaccc gcggccccct tctagacaat gaacaggtcg atatggatat cgcgaccttt
    22261 accatcacgg acgaacgcaa aaaactctac aactttacca gtccctacta cacagacgct
    22321 tctggatttt tggtcaataa atctgccaaa atcaaaaaga ttgaggacct aaacggcaaa
    22381 accatcggag tcgcccaagg ctccatcacc caatgcctga ttactgaact gggcaaaaag
    22441 aaaggtctaa cctttaaatt tgtcgaactt ggttcctacc cagaattgat tacttccctt
    22501 cacgctcatc gtatcgatgc cttttccgtt gaccgctcta ttctgtctgg ctacactagt
    22561 aaacgcacag aactactaga tgatagtttc aagccatctg actacggtat tgttaccaag
    22621 aaatcaaata cagagctcaa cgactatctt gataacttgg ttactaaatg gagcaaggat
    22681 ggtagtttgc agaaacttta tgaccgttac aagctcaaac catctagcca tactgcagat
    22741 taaggaggac accccatgac agatttatca tcttggacag cctattttca ggattttgga
    22801 caatttttca atggtttcct ctttactctt gccctagcga ttggatcctt tatcctcgcc
    22861 atggtcttag gaatcttctt tggtgccatg tcaaccagta aacgtccaat tttacgcatt
    22921 ttagctcgca tctttgtgga attttaccaa aacactcccc tcttggtgca gtttgttatt
    22981 gttttttatg gcctacctct tatcagtgac catactatca tgattccaat ttattggaca
    23041 gctgttctct gcgtgggact ctatcacggc gcttatatcg ctgaggttat tcgttcaggg
    23101 attcagtcta ttcctagtgg tcagatggag gccgccttgt cgcaaggttt tacctatatc
    23161 agtgccatgc gcttgattat cttgcctcag accttccgca tcattctccc tccattgacc
    23221 aaccaaattg ttaacctcat caagaacacc tctactgtag ctatcatctc tggagtagac
    23281 ttgatgtttg tgactaagtc ttggtcggct ctcaacggaa actatatccc agccttttta
    23341 ggtgctgctc ttctctactt tgccctatgc ttccctgttg cccagtttgg tcgcaagatg
    23401 gagcaagcca acaaaaaagc ctattcactt taggaggtta ctatggaatc tattttagaa
    23461 gttttaaccc cagataacct agtctttatc tttaaaggat ttggcttgac cctctatatt
    23521 tctctgattg ccatcatcct ctctactatc atcggtatgg tgctagctgt catgagaaat
    23581 ggcaaaaatc ctgtcttgcg aatcatctcc agtatttata tcgagtttgt gcgcaacgtt
    23641 cccaaccttc tctggatttt tactatcttt ttggtgttca aaatgaaatc cacaccagca
    23701 ggtattacag cctttactct ctttacatca gcagccttgg ctgagattat tcgaggcgat
    23761 ctcaatgccg tagacaaggg acaatacgaa gcaggaatgt cacaaggctt cacctcagcc
    23821 caaatcctct actacatcat tctcccacaa gccatccgca aaatgctacc agccatcatt
    23881 tctcagtttg ttaccgtgat taaggatacc agtctcctct actctgttat cgccctacaa
    23941 gaactctttg gagccagcca aattctcatg ggccgttatt tcgaaccaga gcaggtcttc
    24001 agtctttaca tcctgattgc cctcatctac ttcagcttta acctagcaat ttctagcctg
    24061 tctcatatgc tagccaaacg ttggcaacaa gctgcagaat aatactcttc gaaaatctct
    24121 tcaaaccacg tctgcttcac cttgccgtat atatgttact gacttcgtca gttctatcca
    24181 caacctcaaa acggtgtttt gagcagcctg cgggctagct tcctagtttg ctctttgatt
    24241 ttcattgagt ataaacagcc actctccagt tcaattggag agttttttga tgagagtaga
    24301 tatttataga cctcaatttg acaccataca ttttctatga taaaatgtaa caaatttatt
    24361 tacaacaaat cgaaatctaa atgaaatgga attttctatg aaatcaatca aaggactact
    24421 ctttatcata gctagtttta tcttgactct tttgacttgg atgaacactt ctccccaatt
    24481 catgattcca ggactagctt taacaagcct atctctgact tttatcctag ccactcgtct
    24541 cccactacta gaaagctggt ttcacggttt ggagaaggtc tacaccgtcc acaaattcac
    24601 agcctttctc tccatcatcc tactaatctt tcataacttt agtatgggcg gtttgtgggg
    24661 ctctcgctta gctgctcagt ttggcaatct tgccatctat atctttgcca gcatcatcct
    24721 tgtcgcctat ttaggcaaat acatccaata cgaagcttgg cgatggattc accgcctggt
    24781 ttacctagcc tatattttag gactctttca catctacatg ataatgggca atcgtctcct
    24841 tacatttaat cttctaagtt ttcttgttgg tagctatgcc cttttaggct tactagctgg
    24901 tttttatatc atttttctat atcaaaagat ttccttcccc tatctaggga aaattaccca
    24961 tctcaaacgc ttaaatcacg atactagaga aattcaaatc catcttagca gacctttcaa
    25021 ctatcaatca ggacaatttg cctttctaaa gattttccaa gaaggctttg aaagtgctcc
    25081 gcatcccttt tctatctcag gaggtcatgg tcaaactctt tactttacta ttaaaaattc
    25141 aggcgaccat accaagaata tctatgataa tcttcaagcc ggcagcaaag taaccctaga
    25201 cagagcttac ggacacatga tcatagaaga aggacgagaa aatcaggttt ggattgctgg
    25261 aggtattggg atcaccccct tcatctctta catccgtgaa catcctattt tagataaaca
    25321 ggttcacttc tactatagct tccgtggaga tgaaaatgca gtctacctag atttactccg
    25381 taactatgct cagaaaaatc ctaattttga actccatcta atcgacagta cgaaagacgg
    25441 ctatcttaat tttgaacaaa aagaagtgcc cgaacatgca accgtctata tgtgtggtcc
    25501 tatttctatg atgaaggcac ttgccaaaca gattaagaaa caaaatccaa aaacagagct
    25561 tatttacgaa ggattcaagt tcaaataagt aatcatactc tcctgggatt tcccaggaga
    25621 gttttttata gccaaatgaa ataagacagg aacgatggaa ttttggcatt caaatcaatt
    25681 gataagaatg ttttatagtg gagtgaaata ggatacgaac aaattgatta ggaaaatcaa
    25741 atgaatttat agaaatcttt tagcagttat gttgtcctat tctagtttca aaacgctata
    25801 gaagcagcat tgtgctagtc tagattcagt ttactatact aaaacgagta gcttgaaatc
    25861 aaaaaaccca ccctcacagg caggttttat ctgtattatt cagctagatt atgctttacc
    25921 ttctgaaccg aatacgtcga tacgttcttc aaccgatgct tggatagctt ttacaccgtc
    25981 agccaagaat ttacgtgggt cgaagagttt tttcttgtcg tattctgctt cgtttgcttc
    26041 gtagtcacga gcaaatttac gagttgcgtt agcgaatgcg atttggcatt ctgtgttaac
    26101 gttaactttg gcaacaccaa gtttgatagc tgcttggatt tgctcatcag gaatacctga
    26161 tccaccgtgc aatacgattg ggaatcctgg aagagcttct gtcaattttt gcaagtgatc
    26221 aaggtcaaga ccttcccagt ttactgggta agggccgtgg atgttaccga taccagctgc
    26281 caagaagtcg ataccagttt caaccattgc tttagcgtct tcgattggag ccaattcacc
    26341 tttaccgatg attccgtctt cttcaccacc gatagtacca acttcagctt ctactgagat
    26401 acctttagcg tgtgcttttt caacaacttc tttagccaat ttaaggtttt cttcaactgg
    26461 aaggtgtgaa ccgtcaaaca tgattgaagt ataaccaact tcgatacact caagtgcatc
    26521 ttcgtagtga ccgtggtcaa ggtggatagc tactggtaca gtgataccca ttgattcaac
    26581 aaggttagcg atcaagttgc gagcaacttt gtaaccaccc atgtatttag cagcacccat
    26641 tgaagtttgg atcaaaactg gagctttttt agcttctgct gcgcgcaaga tagcttgagt
    26701 ccactcaagg ttgtttgtgt taaatccacc aactgcataa ccgttgtcac gggctgcttg
    26761 gacaaatttt tctgctgaaa cgattgccat tttatcaggc ctcctgtata tttttatggg
    26821 tcatcccatt tacattgttc attttatcac tttttgccaa aaaaatctag tttttcccgc
    26881 agtttcgatt gattttcttc taactccatc tatgtaaacc ctttctctcc ctagtcttgg
    26941 acgacttttg gaaaatctat aaagaaggtt aaacgattct cctccatctc gaaacgataa
    27001 gctaattttt catgttctaa tagactctta accacaaaga gccccatacc agaccccttg
    27061 accttgcgac tagcattgtc agaaaaagac tgggctagtt tttcttgttc ctctgagcta
    27121 cagctatttt cgataaaaag ttctccttct ctttctccaa ttcgaactaa gccacctgga
    27181 acagagtgct taatggcatt gctgatgaga ttagaaagaa tcaacttcat aactgatggg
    27241 tttagataag cctgctgatg ggtcaaacta ttgtctatct ggagctctct ttccttggct
    27301 agcaaggcat aatctttgac cagattttgc gtcatctgga ggaggtcaat tatttcccta
    27361 tcatctcgca attcctgcac agaagagagg gaaagtatct gcagaacatg gtgattgagt
    27421 tcatccacaa tccccaaggc aactcccaga tactggtctc tatccttata acgaccgata
    27481 ttctctctca tattttcgat taggattttc aaactagcca gcggtgtttt caattcatga
    27541 gaagctcctc gtaggaattc gaccttcatc ttctccagct ggagaatggc ttcattcttt
    27601 tcatgcaagt ccgcaataac agtcaagaga tgctggtaga ggctattgat ttgttccttg
    27661 agattaccta tctcatcctt agaatccacg cgcaatcgca cttgggaatc caggtccatc
    27721 atccgacggg tcacccactt gatttccaaa atcggtgcaa caatagtccg agcgtagatg
    27781 taggccacca agagggaaat cagaaaggag gccagcaagg tatagggaag aaactggaga
    27841 ctgatttgct ccgcttcctt ttgtaaatcc atggaagcta gaaactggag aatcatagta
    27901 ccaccgtctt gcgttttcac ctcgcgctcc tcaataaaga gagaggttgt ctggcggtct
    27961 gtgtccagag gaagactgtc cttgacttct aacttgtcct cggtcatctc acctttgacg
    28021 gtccccttga tatcactagt ctgggaatac aagtctaaca cttgctcgat actctgccta
    28081 tctttccctt ctagggactg ggcaatggct gttgcctttt gaccaatggt ttcctgacga
    28141 tgactcagat aagtcgaagg aaaaagaaaa taaatagcta aatgaaggca gataaccaga
    28201 acactaaata tcgagaaggt atagataaat atctttgcaa ataaacctgt tcgtttcatt
    28261 ttcgctccaa tttataacca acattgcgca cagtgaggat acaatccaag tctagctttt
    28321 tccgcaattc cttgatataa acatcaataa cacggtcaaa gggaacctca tctgtcgctt
    28381 tccagacggc atcgataatc tgagaccgag tcaaggcccg gccttcattt ttcactagat
    28441 agtccagaat ttccaactct ttggcattga taggcacttc ttgacctgcg aggcttgcac
    28501 tgtagctttc aaagtccacc ttggtatcct tgtaagaaaa gattcgtcct gtatcgtagt
    28561 agcgcttgaa aatcgcgtcc accctcactt ttaagaggga gagagagaaa ggtttttcca
    28621 gatagccatc tgccaaagag gcaaaggcac tcatcttgta ttcctcatcc tgaaaagctg
    28681 tcaacatcaa gacaggaacc tgactggttt tacgaatctc agctaggact tctaagccgt
    28741 taagcttggg catctggata tccagtaaaa ccagggccac ctcatagcta gaaaattgct
    28801 ccagagcttc ctgaccgtcc gctgcctcaa tagtttcata gccacaatcc gtcaaataat
    28861 cactgacccc ctcacggatc atctcttcat cttctacaat taaaattttc atactttaac
    28921 tgctctctat tttttatttt tcttagaata aatacctact ctattttcta ttatagtctc
    28981 ttgctggcct tttgtctgta agcaactgat cactagataa aacgttgtga aattcctttc
    29041 tcataaattc cataacttta gtatattata tttaagcact aaagtacaaa gaaagcaact
    29101 gaaagcaatg attttcacca ctgctttcag atttattttg aattgttaaa tagctattcc
    29161 tatccactat tcttgaatag aaacacaaga tgcaatcttt attccagact cattttttca
    29221 aatttattca ccatccagca agagctcttt tggttgtttt ctaaggagat tgcttgaagc
    29281 aagcgccata acgagaacca ctagaaccaa ggcaaggaca aaaatgatga taaagtctga
    29341 tgtctgaatg gaaatgtcta ggctcgacaa ggtcttgcta aagccatcta cttctgcacc
    29401 gccaccaagg ttagaggctt gagccgcctt actagcctgt ttggcaacac ctgaagtcac
    29461 attggcaagg acagtgtttc caattgcacg ggcagtgtaa ttagctagga agtaagcaga
    29521 aactagagca gggatagcaa tcaagataga ttcggtgatg aattgaccca agatacttgc
    29581 ctgcttgagg ccgatagaga ggagaattcc cacttccttg cgacgggcgt tgatccaaag
    29641 gctgagcaag agggcaagga ggagaactga gaagctcaag ctaccccaga agaggaggtt
    29701 ggccatcttg tacataccag agatagattg ctcaagagct gggtagttag aggagctctt
    29761 gacgagtgtg tagctcttcc agttgatacc actgatgcca ttcaactctt tcataacatc
    29821 atccaagttc ttgtctgctg ttacaaagaa ggttgcgtcc ccataaatgg ctgtgtcttc
    29881 tgtgtatcca taaagttttg cagcagtgtg aatgtctgta atagctgtgt tttcgtaaag
    29941 ttcttgtgag taggttactg ctgacttatt atgaccatca aagagtccct tgattgtcac
    30001 ttcaactgtt tccttggctc ctttttcatt atctgcatcg tagatattag agtccagttt
    30061 aaccttgtcc cctactttcc agccgtgttt ggctgccaag tccttgtgca agaggatttt
    30121 atccttgtcg tcgttggtta agtgctctcc ttcgactagt ttataagaac cagagacaaa
    30181 cttatcttct ttagaggagt cattgacacc tgtaatcatc aagctacttc caaaacgctt
    30241 ggcacgatca gcagtgagat tcttcttggt ttctggcgtt tcaatcaggt catatccagt
    30301 caaatctccg atagcgttga tacgtttgac ataagactcg atggccttgt tttcggtgat
    30361 ttttttgatg tcttcaccct tgatattccc agcaccacga ggcgttcctt ggttgacgcg
    30421 acgattgatt tgcatggaga agctattggt gatattttta aaggtctcct gagaagcctt
    30481 ggcagtagct cccttgattg acaagccgac caaactcaag ctcgccatga ggagaataat
    30541 caggaagatg acaatcgatt tgaaaaactt ccttgtaaca taggcaaatg cgttgtgtaa
    30601 catagattcc ctttctagat tttgttttaa tcattctatc aaaataagct caaattattt
    30661 actagtattg cgcgtttcag tcagtttctt atcctttaat tcaagtgtaa tatctgacgc
    30721 ttgtgccact tctttactgt gagttacgac aatcacacat ttacctgttt tctgggcaag
    30781 tgatttgagt agttcgacaa tatctccagc agttttagga tccagatttc ctgttggctc
    30841 atcagctaga ataactggag cttctgagac caaactgcga gcaatggcaa cacgttgctg
    30901 ttgaccacct gataactgga gaacattccg cttgatctgg ctttcatcca aaccaagctc
    30961 aagaagtgta ttcttgcttg cctttttgtt gaccaatcgg atattttcca gcggagaaag
    31021 ataatctatc aagttataat tttgaaagac cagggaaata tggtgcatgc gatggtaaga
    31081 atagcccttc ttacgaatat cctctccttg aaaaaggata gaaccttcaa caggactatc
    31141 tagaccagca agtagggaca agagtgtgga ttttcctgct cctgactccc caataatact
    31201 gtaaaatttt ccgggttcaa aattataatc gatctgatat aggactgctt cagcagtatt
    31261 cttataacgg taggtaacat cttgtaattg taataaagtc atgatttctc cttcttaact
    31321 aatagatgat aaaatttctt tcggtgattt tctaaataag aataggaaac aaagggctac
    31381 agataagcaa ctaagcagaa ctagaaaaac ataggattct gcaaaagata agatactagt
    31441 tgataaactg cttgctttgg ctagtgtatc ttgtaagctt gcctgatctc cacttgctag
    31501 tagagtttgg agtaggtaag ttgtgattcc gtttcctgca acaaatgctg gaagcaaagc
    31561 tccaagagat accaaaacta cctctaaaca gaattgtagg aagatcgagc tcttgccttt
    31621 tccaagtgca agtaaaatcc ccacttcata gacccgttct ctcaaccaga gagacaaaac
    31681 cagaattaag gctccagctc ctgctatcaa catcccataa aggaagatgg tcaggaaggt
    31741 ttggaaagtt gcaactgagt ctttgatttg ttcaaaagcc ttgttttcct tttcgacttg
    31801 gtagccttga ttttccaagg ccaagttttc tacctgcttc atgagtccgt ccatttcctt
    31861 aggattttct acatagaagc gtgctgcact gacttgagct tcactattgc ccaaaagggt
    31921 ttggctactt tcatagtctg taaagacttg attttcactg aagtcagaag acaagcctgt
    31981 gaatttctct tgttttttac cagaaaagat gccgataatc tcaaactcta ctgtttgtcc
    32041 ttttccagat tcagactgac cagcatccaa gccaatcttg tcatgaagtg aaagaccgtt
    32101 cttcttagcc aattcttcgt ggataaggat tttcttggaa tccccttttt gaaggtgtcg
    32161 cccttctttt agattgaaag ccgaactggt aaaggttaca tccttggatg aatcctcaag
    32221 agccgttaag ctaaccaagt tattgtctgc agctgataaa tcatcacgct ccacgctctg
    32281 ctcgccagtc actgcttcct tgtcttttag ttttgcgacc gtctcaagtt caggagagac
    32341 attttccagc cccttaatct tgcttacaga tgctaggtct gacaacttga atgtctgacc
    32401 attctctatc ttcttaatag aaaaagatgt attgagtgat ttataaagat tgctttctac
    32461 tgttttgttg gacttcatca gagtcaaaca ggctgaaatt ccggccaata agaccaataa
    32521 aatcagaaat aaaataaaac ttctcagtcg ctttctgctg acataagccc aagatctttg
    32581 gattggattc atttgtcacc tccatatttg taagactatt ataaaaccca aatatgaaat
    32641 gtttatgaaa tacgaaaaaa aa
//
LOCUS       DAVGJL010000010        29727 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.010, whole genome shotgun sequence.
ACCESSION   DAVGJL010000010 DAVGJL010000000
VERSION     DAVGJL010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29727
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.010"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            354..1517
                     /locus_tag="VVE42_000388"
     CDS             354..1517
                     /locus_tag="VVE42_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEU3072649.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASDLENVNLKDREVEVQGQEGN
                     FLGTAYLSQQNKGLGWFISKDKVTFNQAFFETLFRKAKEKRNAYYQDDLTTAFRLFNQ
                     EGDGFGGLTVDLYGDYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGFDY
                     ESAHVYGQEAPDFFTVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLAMGKSLLN
                     MFSYTAAFSVAAAMGGASQTTSVDLAKRSRELSQAHFQANGLSTDEHRFIVMDVFEYF
                     KYAKRKDLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSRQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            1514..2191
                     /locus_tag="VVE42_000389"
     CDS             1514..2191
                     /locus_tag="VVE42_000389"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEU3072650.1"
                     /translation="MKLIVSVMPRSLEEAQALDATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQLYQPDYIDFEYYSYKD
                     VFEEMLDFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEVSAPGQISLASMKKI
                     REILDEA"
     gene            2181..3035
                     /locus_tag="VVE42_000390"
     CDS             2181..3035
                     /locus_tag="VVE42_000390"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEU3072651.1"
                     /translation="MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGAYVAWEI
                     EASDLAETVANIRRYQMFGVNLSMPYKEQVIPYLDELSDEARLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGMDGQSSPVPENIVL
                     PETLLVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIW
                     QSLTEKYQ"
     gene            3054..4121
                     /locus_tag="VVE42_000391"
     CDS             3054..4121
                     /locus_tag="VVE42_000391"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEU3072652.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCMAQVGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVMIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTGLNGSVESILEHAETLI
                     EHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     VAEEKGLMPAGITQSITEMCQKFGLPVDYENWEVDKLYQALTHDKKARGNTLKLVLVP
                     ELGSAIIHPVSLEEMKDYLVK"
     gene            4131..5297
                     /gene="aroC"
                     /locus_tag="VVE42_000392"
     CDS             4131..5297
                     /gene="aroC"
                     /locus_tag="VVE42_000392"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEU3072653.1"
                     /translation="MRYLTAGESHGPRLTAIIEGIPAGLPLTAEDINEDLRRRQGGYG
                     RGGRMKIESDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY"
     gene            5307..6410
                     /locus_tag="VVE42_000393"
     CDS             5307..6410
                     /locus_tag="VVE42_000393"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEU3072654.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSETILDRIEDFKERLEVIGQAISKGDEEQIWNFFN
                     QAREQRQTMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEHLITENTDYTVVIK"
     gene            6421..6759
                     /locus_tag="VVE42_000394"
     CDS             6421..6759
                     /locus_tag="VVE42_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEU3072655.1"
                     /translation="MSNIYDSANELSRGLRGLPEYKAVKAAKDAIAADAEASKIFTEY
                     LAFQEEIQKLAQTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            6851..8134
                     /gene="aroA"
                     /locus_tag="VVE42_000395"
     CDS             6851..8134
                     /gene="aroA"
                     /locus_tag="VVE42_000395"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEU3072656.1"
                     /translation="MKLKTNIRHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVITIQGVGMAGLKAPQNALNMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLKGTKNLRPIHY
                     ELPIASAQVKSALMFAALQAKGESVIIEKECTRNHTEDMLKQFGGHLSVDGKKITVQG
                     PQKLTGQKVVVPGDISSAAFWLVAGLIVPNSRLVLQNVGINETRTGIIDVIRAMGGKL
                     EITEIDPVAKSATLIVESSDLKGTEICGALIPRLIDELPIIALLATQAQDVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVAGGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            8127..8603
                     /locus_tag="VVE42_000396"
     CDS             8127..8603
                     /locus_tag="VVE42_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEU3072657.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDPNYLDMDALIEKRLGMSIANFFA
                     EKGEAAFRQVESEVLADLLQTDQVVSTGGGVVISQRNRDLLKTNTDNIYLKADFETLY
                     QRIAADKDNQRPLFLNNSKEELVAIFQERQAWYEEVASRVLDVTKLSPEEIIEELR"
     gene            8600..9448
                     /gene="pheA"
                     /locus_tag="VVE42_000397"
     CDS             8600..9448
                     /gene="pheA"
                     /locus_tag="VVE42_000397"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEU3072658.1"
                     /translation="MKIAYLGPKGSFSHHVVQTAFPHEELQAFANITDVIKAYEQGLV
                     DYSVVPVENSIEGSVHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAVAPRSSAEEYGLELIA
                     EDIQEMEANFTRFWVLGAEKPSIPLQAQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESRPLKTALGEYFFLIDVDYIDKDLVHFAQKELEAIGIQYKILGAYPIYPISD
                     HGKERR"
     gene            9445..10719
                     /gene="psr"
                     /locus_tag="VVE42_000398"
     CDS             9445..10719
                     /gene="psr"
                     /locus_tag="VVE42_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HEU3072659.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFAYLQEKLTLAR
                     GNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSFPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMVFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            10998..11843
                     /locus_tag="VVE42_000399"
     CDS             10998..11843
                     /locus_tag="VVE42_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU3072660.1"
                     /translation="MNNMTDLKAIQDRSLEMAEYFVAFCKEHDLLCYLCGGGAIGALR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSYKDFVDRNLFITIRDKET
                     TCIKPYQQDLDLPHGLALDVLPLDYYPKNPAERKKQVRWALIYSLFCAQTIPEKHGAL
                     MKWGSRILLGLTPKSLRYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EDNLFLPFEGTEMPIPIGYDVYLRTAFGDYMTPPPADKQVPHHDTVIADMDKSYTEYK
                     GEYGG"
     gene            11833..12936
                     /locus_tag="VVE42_000400"
     CDS             11833..12936
                     /locus_tag="VVE42_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU3072661.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDPEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLARRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNS
                     IKEVYPDYASKLQTIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLHQEGKNYHLYFIGAGDMEEELKKRVKEYGIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTDVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFDVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            12938..13924
                     /locus_tag="VVE42_000401"
     CDS             12938..13924
                     /locus_tag="VVE42_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU3072662.1"
                     /translation="METALISVIVPVYNVAQYLEKSIASIQKQTYQNLEIILVDDGAT
                     DESGRLCDSIAEQDDRVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKLIKRQIATDLSFPKGLIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAEKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAHFFILDKMLLDDQYKQFEAYS
                     QIHRFLKGHAFAISRNPIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            13940..15373
                     /locus_tag="VVE42_000402"
     CDS             13940..15373
                     /locus_tag="VVE42_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HEU3072663.1"
                     /translation="MIDGKRLLFSLTIVSYALTLVSGVVYLFNNNNVSLLSTLLFLLV
                     SSLIACWNDIKYYLIHFIFYLTIFVFLVSRPTIDYFRDGALDTYHPIAYRFAFIVVMV
                     SILGLTTGGILARYFIARKKIKVANIGNSLKEVYIKRLRFVSLGVFLLTYPFYFIRLF
                     ERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYLATKPKKLQATAVLVSFI
                     AANTIHLAIGTRNPFILSILFAFVYYFMREQTEKGKWIGFKEKLAIFVGSPILMLAMG
                     VLNYVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFNSSLPYRDFRNFTFGPVL
                     DYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLVLNKEYLKGHGIGSSYIM
                     ELYTDYGMIGVFLLSFLLGVLFIAMLQVAYRSRTILFALSLLILNNLFFMPRSSFSES
                     FFNLFTMQFWGIVLVIIFVAKMLTKEN"
     gene            15402..16436
                     /locus_tag="VVE42_000403"
     CDS             15402..16436
                     /locus_tag="VVE42_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEU3072664.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPKESAKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANDLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            16582..17319
                     /gene="mecA"
                     /locus_tag="VVE42_000404"
     CDS             16582..17319
                     /gene="mecA"
                     /locus_tag="VVE42_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEU3072665.1"
                     /translation="MKMKQISDTTLKITISLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEAKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVAFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFAEDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            17470..18756
                     /locus_tag="VVE42_000405"
     CDS             17470..18756
                     /locus_tag="VVE42_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEU3072666.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLAAGNDFNFVTNVDDILSDQDITIVVELMGRIEPAKTFITRALEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKITRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVAHKGIRNITPEDVAVAQELGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILAPDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            18758..19627
                     /locus_tag="VVE42_000406"
     CDS             18758..19627
                     /locus_tag="VVE42_000406"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEU3072667.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHD
                     LRVDTQGVRVEAK"
     gene            19703..19780
                     /locus_tag="VVE42_000407"
                     /pseudo
     CDS             19703..19780
                     /locus_tag="VVE42_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            19968..20906
                     /gene="msrB"
                     /locus_tag="VVE42_000408"
     CDS             19968..20906
                     /gene="msrB"
                     /locus_tag="VVE42_000408"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEU3072668.1"
                     /translation="MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLL
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
     gene            20964..22688
                     /locus_tag="VVE42_000409"
     CDS             20964..22688
                     /locus_tag="VVE42_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3072669.1"
                     /translation="MKHLLSYFKPYIKESILAPLFKLLEAVFELLVPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGFAKELTNDLYRHILSLPKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISAELTFWFL
                     VMVAILTIVIVGLSRLVNPLYSSLRKKTDQLVQETRQQLQGMRVIRAFGQEKRELQIF
                     QTLNQVYARLQEKTGFLSSLLTPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLINSLNQSYISAKRIEEVFAESPENIHSELEQKQVTSGRVLQV
                     QELTFTYPDAAQPSLRDISFDMTQGQILGIIGGTGSGKSSLVQLLLGLYPVDKGNIDL
                     YQNGRSPLNLEQWRSWIAYVPQKVELFKGTIRSNLTLGLNQEVTDQELWQALEIAQAK
                     DFVSDKEGLLDALVEAGGRNFSGGQKQRLSIARAVLRQAPFLILDDATSALDTITESK
                     LLKAIRENFPNMSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYREIN
                     ASQHGKED"
     gene            22690..24438
                     /locus_tag="VVE42_000410"
     CDS             22690..24438
                     /locus_tag="VVE42_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3072670.1"
                     /translation="MKRQTVNQTLKRLVVDLANHPFLLFLAFLGTIAQVGLSIYLPIL
                     IGQVIDQVLVAGSSPVFWQIFLQMLLVVIGNTLVQWANPLLYNRLIFSYTRDLRERII
                     HKLHRLPIAFADRQGNGEMVSRVTTDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKRSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGST
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     SDQVKGAISFKHVSFGYHPEKILIKELSIDIPAGSKVAIVGPTGAGKSTLINLLMRFY
                     PISSGDILLDGQSIYDYTRVSLRQQFGMVLQETWLTQGTIHDNIAFGNPEASREQVIA
                     AAKAANADFFIQQLPQGYDTKLENAGESLSVGQAQLLTIARVFLAIPKILILDEATSS
                     IDTRTEVLVQDAFAKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMDR
                     KGKYYQMQKAAAFSSE"
     gene            24785..26044
                     /locus_tag="VVE42_000411"
     CDS             24785..26044
                     /locus_tag="VVE42_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HEU3072671.1"
                     /translation="MKVFQHVNIVTCDQDFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIIDYQGAWIMPGLVNCHTHSAMTGLRGIRDDSNLHEWLNDYIWPAESEFTPDMTT
                     NAVKEALTEMLQSGTTTFNDMYNPSGVDIQQIYQVVKTSKMRCYFSPTLFSSETETTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIERLASSQVAIAHNPI
                     SNLKLASGIAPIIQLQKAGVAVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKVLGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSNDVDDVYIAGEQVVKQGQVLTVEL"
     misc_feature    26120..26255
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            26294..26794
                     /locus_tag="VVE42_000412"
     CDS             26294..26794
                     /locus_tag="VVE42_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEU3072672.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNDFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     gene            26870..27238
                     /gene="rplL"
                     /locus_tag="VVE42_000413"
     CDS             26870..27238
                     /gene="rplL"
                     /locus_tag="VVE42_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEU3072673.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKAKLEEAGASVTLK"
     gene            27426..27596
                     /locus_tag="VVE42_000414"
     CDS             27426..27596
                     /locus_tag="VVE42_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3072674.1"
                     /translation="MQKEGQSSRQFVKSHLMTIMGVDIVKATQLVDEMEQVGLIRFDE
                     FGNVGILVLEGQ"
     gene            27596..27847
                     /locus_tag="VVE42_000415"
                     /pseudo
     CDS             27596..27847
                     /locus_tag="VVE42_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            27834..28181
                     /locus_tag="VVE42_000416"
                     /pseudo
     CDS             27834..28181
                     /locus_tag="VVE42_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645893.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            28174..28554
                     /locus_tag="VVE42_000417"
     CDS             28174..28554
                     /locus_tag="VVE42_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEU3072675.1"
                     /translation="MIDLYLSKNRQRNQLLLDFFQNYGIEVSCHSVSEMTKDKLIEMM
                     SYSSDCFEFLSPNLLRFKNRDNLRLTDFVEIILKNPELTIRLPLAVSNKRVYPSLNLE
                     EARALLPRDTKQLIYMAQTHYLSN"
     gene            28567..28710
                     /locus_tag="VVE42_000418"
     CDS             28567..28710
                     /locus_tag="VVE42_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3072676.1"
                     /translation="MAERFWENLSIILAERNISCIELTRKMFAGEFHYPSELNRLYQK
                     IRH"
     gene            28803..29390
                     /locus_tag="VVE42_000419"
     CDS             28803..29390
                     /locus_tag="VVE42_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU3072677.1"
                     /translation="MKRIIPVYIFQQVNVLLVSLYLLKFLCIGELTILQILYGSSLIS
                     FLWMYGQRKQAHKVNMKSRMKWLGIGFVSLLIISLCFSLIHAQESTNQANLIGLQHQV
                     PWFSFLLFLINASMVEEFLYREILWNLVRKLDIRVALTSVLFALAHHPGTIIAWCLYV
                     SLGMFLGLVRYKLDLWGSMGLHLVWNLLVYSLLLF"
     gene            29592..>29727
                     /locus_tag="VVE42_000420"
     CDS             29592..>29727
                     /locus_tag="VVE42_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3072678.1"
                     /translation="MAEEVLNLQLVSVQVDETDEVDGMRFSTFSTNRCGNWSAFSWEN
                     C"
BASE COUNT         8833 a         5454 c         6684 g         8756 t
ORIGIN      
        1 aaaaggcttt tctataaaat caaattttcc agatatctac tgaagctgat ttatttcttc
       61 atctttcttc tactaccttt gtcactaaat ttgacagaca catattgctg atacaacctc
      121 tctacctata atctcacggt taacctgagg ctagtttcat agtttgctat ttgattttcg
      181 ttgataaaaa gaaatgctcc tccaagacgg aagagcattt tttattgtga ttcttacaca
      241 tttatttcct agttttgcag gaagtctagt ataccatttt ttaaaagaag agtaaaaaag
      301 caagggtagt ttcttttttg agaattgtct aaaagtttga tataatggta gttatgaata
      361 gaataagagt cagcaaaagg gttgaaaaga agcttgctaa ggggctagtt ttactagaag
      421 ccagtgatct tgagaatgtc aatcttaagg atcgggaagt agaggtgcag ggtcaggaag
      481 gaaactttct tgggactgcc tacctttctc agcaaaacaa gggcttgggc tggtttatca
      541 gcaaagacaa ggtgaccttc aatcaagctt tctttgaaac gttgtttaga aaagccaaag
      601 aaaagagaaa cgcctactat caagatgatt tgacaactgc ctttcgtctc tttaatcaag
      661 agggagatgg ctttgggggt ctgacagtgg acctttatgg cgactacgcc gtcttttctt
      721 ggtataactc ttatgtttat cagattcgtc agactatatc agaagccttt agacaggttt
      781 tccctgaggt tttaggggct tatgagaaaa tccgctttaa gggttttgac tatgaatctg
      841 cccatgttta tggtcaagaa gcacctgact ttttcactgt tttagaaaat ggtgtcctgt
      901 atcaagtctt tatgaatgat ggcttgatga cagggatttt cctagaccag catgaggttc
      961 gcggtagttt agttgacggc ttggctatgg gtaaatcctt gctcaatatg ttttcctaca
     1021 cagcggcttt ttcagtagct gcggccatgg gaggagctag ccagacaact tctgttgatc
     1081 tagccaaacg ttcacgagaa ttgtctcaag cgcattttca ggcaaatggg ctcagcacag
     1141 acgagcatcg ttttatagtc atggatgtct ttgagtattt caaatatgcc aaacgcaaag
     1201 acttgaccta cgatgtgatt gtcctagatc cgcctagctt tgctcggaat aaaaaacaaa
     1261 ctttctctgt ggccaaggat tatcacaagt tgatttccca gagtcttgag attttaaatc
     1321 cgggagggat tatcattgcc agtaccaatg ctgccaatgt ttcccgtcag aaatttacag
     1381 aacaaattga taaaggtttt gcaggaagaa gttaccagat tttaaacaaa tatggtcttc
     1441 cagcagattt tgcctataat aaaaaagatg aaagtagtaa ttacctcaag gtgattagta
     1501 tgaaggttag taaatgaaat taatcgtttc agtaatgcca agaagtttag aggaggctca
     1561 ggctctggat gccacgaggt acctggatgc cgacatcatt gaatggcgtg ccgactatct
     1621 gcctaaagaa gcgattttgc aggtagctcc agccattttt gaaaaattcg caggccgtga
     1681 gttggttttc acgctacgaa ctcgctccga aggtggagaa atcgaccttt ctccagaaga
     1741 gtatatccat ctaatcaagg aagttgcgca actctatcaa ccagactata ttgattttga
     1801 gtactatagc tacaaggatg tttttgagga aatgctggac ttcccaaatc ttgttttgag
     1861 ttaccataat ttccaagaaa cacctgagaa catgatggaa atcttgtcag agttgacgat
     1921 cctaaatcca aaacttgtta aggttgcggt gatggctcac acggagcagg atgtcttaga
     1981 cttgatgaac tatacacgag gctttaaaac cctcaatcct gaacaggaat atgtgaccat
     2041 ttctatgggt aaggtgggca aggtctctcg tatcactgcg gatgtgactg gttcgagttg
     2101 gtcttttgcc agtctggatg aggttagtgc ccctgggcag atttctctag ctagcatgaa
     2161 aaaaatcagg gagattttgg atgaagcttg atggctatac acgtttagct gcagttgttg
     2221 ccaatcctat taagcattct atttctccct tcatccacaa tagagccttt gaggcgacag
     2281 ctaccaacgg tgcttatgtg gcttgggaga ttgaagcgag tgacttggca gaaacagtgg
     2341 ccaatattcg tcgctaccag atgtttggcg tcaatctgtc catgccctat aaggagcagg
     2401 tgattcctta tttggatgag ctaagcgatg aagcgcgctt gattggtgcg gttaatacgg
     2461 ttgtcaatga gaatggcaat ttaattggat ataatacaga tggcaaggga ttttttaagt
     2521 gcttgccttc ttttacaatt tcaggtaaaa agatgaccct gctgggtgca ggtggtgcgg
     2581 ctaaatcaat cttggcacag gctattttgg atggcgtcag tcagatttcg gtctttgttc
     2641 gttccgtttc tatggaaaaa acaagacctt acctagacaa gttacaggag cagacaggct
     2701 ttaaagtgga tttgtgtgct ttagaatatg tttctgaact gcaagcaagg attgccgagt
     2761 cggatttgct agttaatgcc accagtgtgg gcatggatgg ccaatcctct cctgttcctg
     2821 aaaacatagt cttaccagaa actctcttag ttgcagatat catataccaa ccctttgaaa
     2881 caccgttttt gaaatgggct agaagtcagg gcaatccagc agtcaatggt ctgggaatgt
     2941 tgctctatca agctgcagaa gcttttcaac tgtggacagg caaggaaatg ccgacagaag
     3001 agatttggca gtctttaaca gaaaaatacc aataaagaaa gggagactct cctatgaaaa
     3061 tcagaatcga tattcctcat catccttatg atattcagat tgaaaaaggt tgtatggccc
     3121 aggttggtca gtggttgcga gaactctggc aaccgcaaaa ggtagtcatt gtgacagata
     3181 accatgtagc ctctctctat gcagagaagg tcaagctcag cctagaagat gctggttttc
     3241 aggtagctgt ttttgatttc ttagaaggtg aagaaagaaa gaatttaact actgttcaga
     3301 aagtctatga atttctagtc aagcaaggtc tgactcgtag cgatggaatc gttgctcttg
     3361 gtggtggcgt tgttggggac ctggctggct ttgtagcctc tacctatatg cggggtattc
     3421 actttgttca gattccgact agtttgacag ctcaggttga ttcttctatc ggtggaaaga
     3481 caggcgttaa cacaccattt gctaaaaata tggtggggac ctttgcccaa ccagatgggg
     3541 ttatgattga tccacttgtt cttgaaaccc tcggaaaaag agagttgatt gaagggatgg
     3601 gtgaagttat caagtatggc ttgattgagg atccagaact gtgggctctc ttgacgggac
     3661 tgaatggttc tgttgagagt attttggaac atgcagagac cttgattgaa cattcttgtc
     3721 aggtgaagcg caagatggtg gttgaagatg agttggacaa tggtattcgt ctttacctca
     3781 attttggcca cactattggc catgccatcg aagcgactgc cggttatggc aaggtcatgc
     3841 atggagaggc tgttgccatg ggaatggtac agatttccaa ggttgctgag gaaaaaggcc
     3901 tcatgccagc tggcattacc caatctatca cagagatgtg tcagaaattc ggcttgcctg
     3961 ttgactatga aaattgggaa gttgacaagc tttatcaggc tcttactcat gacaagaaag
     4021 cgcgtggtaa caccttgaaa ttggtcttgg tgccagagct tggttcagcg atcattcacc
     4081 cagtttctct ggaagagatg aaagactact tggtaaaata aggagagtgt atgagatatt
     4141 taactgcagg agaatcacac ggcccccgtc taacagctat tattgaggga attccagctg
     4201 gacttccatt gacagctgag gatatcaatg aggaccttag acgccgtcag ggtggctacg
     4261 gtcgtggtgg tcgtatgaag attgagagtg accaggttgt ctttacttcg ggcgttcgcc
     4321 acgggaagac gacaggggcg cctattacta tggatgtcat caataaggac caccagaaat
     4381 ggctggacat catgtctgcg gaggacattg aagaccgcct taaaagcaag cggaaaatta
     4441 ctcatcctcg cccaggtcat gccgatttgg ttggggggat taagtaccgt tttgatgatt
     4501 tgcgaaattc tttggagcgt tcatcagctc gtgaaaccac catgcgggtg gcagttggtg
     4561 cagtagccaa acgcctctta gctgagctgg atatggagat tgccaaccat gtcgtggtct
     4621 ttggtggcaa ggaaatcgat gttcctgaaa atctgacagt cgctgaaatt aagcaaagag
     4681 ctgcccagtc tgaagtttct attgtcaacc aagaacgaga acaggaaatc aaggactata
     4741 ttgaccaaat caaacgtgat ggtgatacca tcggtggggt tgtggagaca gtcgtcggag
     4801 gcgttccagt tggtcttggt tcctatgtcc aatgggatag aaaattggat gcaagattgg
     4861 ctcaagctgt tgtctctatc aatgccttta aaggggtgga atttggtctt ggctttgagg
     4921 ctggttatcg taaaggcagc caagttatgg atgaaattct ctggtctaaa gaagacggtt
     4981 atactcgccg taccaataat ctaggtggtt ttgaaggtgg tatgactaat gggcaaccca
     5041 tcgttgttcg tggggtcatg aaacccattc ctactcttta taaacctctt atgagtgtgg
     5101 atatcgaaac ccacgaacct tacaaggcaa ccgtggagag aagtgatccg actgctcttc
     5161 cagctgcagg aatggtcatg gaagcagttg tagcaacggt tctggcgcaa gaaatcctcg
     5221 aaaaattctc atcagataat cttgaggaac taaaagaagc ggtagccaaa caccgagact
     5281 atacaaagaa ctattaagga gttcctatgg caaaaacaat ctatatcgca ggtcttgggt
     5341 tgattggagc ctctatggca cttggtatca aacgcgatca tccagattat gaaattttag
     5401 gttataatcg tagtcaagct tcgagagata tcgccttgaa agaaggtatg attgaccgtg
     5461 caacggatga ttttgctagt tttgctcctt tggcagatgt cattatcctc agcttgccaa
     5521 tcaaacaaac tattgctttc attaaggagt tggccaattt ggatttgcga gaaggcgtta
     5581 ttatttcaga tgctggttcg accaagtcaa ccattgtgga tgcggcggag cagtatttgg
     5641 ctggcaagtc tgttcgcttt gtcggggccc atcccatggc tggtagtcac aagacagggg
     5701 ctgcttcggc agatgtcaat ctttttgaaa atgcctatta tatctttaca ccttcaagcc
     5761 tgacaagtca ggacacgctt aaggaaatga aggatctgct ttcaggtctt catgctcgtt
     5821 ttatcgagat tgatgccaag gagcatgatc gtgtcacttc tcagattagc cattttcctc
     5881 atattttggc ttctagtctc atggagcaga ctgcggtcta tgctcaagag catgagatgg
     5941 caaggcgctt tgcggcaggt ggttttcgag atatgacccg aattgcggaa agcgagccag
     6001 gaatgtggac ctccattctc ttgtccaata gcgagaccat tctggataga attgaggatt
     6061 tcaaggaacg tttggaagtg attggtcagg ccattagtaa gggagatgaa gagcaaattt
     6121 ggaacttttt taaccaagcg cgtgaacaac gccagaccat ggaaatccat aagcgtggtg
     6181 gtgtggatag ctcttacgac ctctatgttg acgttcctga tgaagaagat gtcatcctgc
     6241 ggattttgga actgctacgt ggaacttcct tggttaacat ccacatcaac gaggaaaatc
     6301 gtgaggatat tcacggaatc ctacaaattt catttaaaaa tgctcaagac ttggaaagag
     6361 ctgagcatct cataacagaa aataccgact atacagtcgt catcaaataa ggagaagaac
     6421 atgtcaaata tttacgatag tgcaaacgaa ctcagtcgcg gtctacgcgg attaccagaa
     6481 tacaaggctg ttaaagcagc taaagatgcg attgcagcag atgctgaggc aagcaaaatc
     6541 tttacagaat atcttgcctt tcaagaggaa attcaaaaac tagcacagac aggtcaaatg
     6601 ccagatgcgt ccttccaagc taagatggaa ggctttggta aacagattca aggaaatagc
     6661 ctcttgtcag aattctttac caagcaacaa caattggcca tttacctttc tgacattgaa
     6721 aaaattgttt ttgaaccagt ttcagaattg ctgaaataag aaaagaacga tcctaaagtc
     6781 aggaattttt aaggaatttc tgacttttta tgataaaata ggtaaaagta ttgcaagtat
     6841 gaggtccagt atgaaactaa aaacaaacat tcgccattta catggtagta tccgcgtccc
     6901 aggtgacaag tctatcagcc accgttccat tatctttgga agtttggctg agggtgagac
     6961 caaggtttat gatattctgc gaggtgaaga cgttctttcg accatgcagg tttttcgtga
     7021 ccttggtgtt gaaattgagg ataaagatgg ggttattacc attcaaggtg taggcatggc
     7081 tggcttaaaa gcgccacaaa atgcccttaa tatgggaaat tctggcacct cgattcgcct
     7141 gatttcaggt gtccttgctg gtgcagattt cgaagtagag atgtttggag atgatagtct
     7201 ttccaaacgt cctatggacc gtgtgaccct tccactgaaa aaaatgggcg tcagcatttc
     7261 agggcaaact gaacgagact tgcctcccct tcgcttaaaa gggacgaaaa acctaagacc
     7321 tattcattat gagttgccaa ttgcctctgc ccaagtcaag tcagccttga tgtttgcagc
     7381 cttacaagct aagggggagt cagttattat cgaaaaagag tgcacccgta atcatactga
     7441 agatatgttg aaacaatttg gtggtcattt aagtgtggac ggtaagaaaa tcacagtcca
     7501 agggccacaa aaattgacag gacagaaggt ggtcgtacca ggagatattt ccagtgcagc
     7561 cttttggtta gtcgcaggtt tgattgttcc aaattctcgt ctagtgctgc agaatgtggg
     7621 gatcaatgaa actcgcaccg gtattattga tgtcattcgt gccatgggtg gaaaattgga
     7681 aataactgaa atcgatccag tcgctaaatc tgcaaccttg attgttgagt cttctgactt
     7741 gaaaggaaca gagatttgtg gcgctttgat tccacgtttg attgatgaat tgcctattat
     7801 tgccctactt gcgacccaag cccaagatgt aacagttatc aaggatgctg aggagctcaa
     7861 ggtcaaggaa acagaccgta ttcaggttgt ggcagacgcc ttaaatagta tgggagcaga
     7921 tattactcct acggcagatg ggatgattat caaaggaaaa tcagctcttc acggtgctag
     7981 agtcaatacg tttggtgacc accgtatcgg catgatgaca gctatcgcag ccctattggt
     8041 tgcaggtgga gaggtggagc ttgaccgtgc agaagccatc aataccagct atcctagttt
     8101 ctttgatgat ttggagagct tgattcatgg ctaaggtgtt attagggttt atgggggctg
     8161 gaaaatcgac tattgcaaga ggcttggacc ctaattacct tgatatggat gctctgattg
     8221 agaagcgcct aggtatgtcc attgcgaatt ttttcgctga aaagggagaa gcagcctttc
     8281 gtcaggtaga atcagaagtc ctagctgatt tactacaaac agaccaagtc gtgtcaactg
     8341 gaggaggagt ggttatttct cagagaaatc gtgacttact caagactaat acagataaca
     8401 tctacctgaa agcagatttt gaaaccctct accaacgtat cgcagctgat aaggacaatc
     8461 agcgaccgct ttttctaaat aatagcaagg aagaactagt agctattttt caagaaagac
     8521 aggcttggta tgaggaagtg gctagtcggg ttttggatgt gaccaagcta agcccagagg
     8581 aaattataga ggaactaaga tgaaaattgc ttatctaggt cccaaaggat cattttcaca
     8641 ccacgttgtg cagacagctt ttcctcatga ggaattgcag gcttttgcca acattacaga
     8701 tgtcatcaag gcttatgagc aaggattagt ggactattct gtggtgccag ttgaaaattc
     8761 tattgagggt agtgttcatg aaaccttgga ctatcttttt catcaggctc acatccaagc
     8821 agtagcagaa atcgttcagc ctattcatca gcagttgatg gtggttccag gccatactaa
     8881 gattgaaaag attttttcac atccacaggc cttggctcaa ggaaagaaat tcatcgatga
     8941 acaatatcca gaggctcaaa tcgaggtaac agctagtaca gcttatgcgg cccgttttat
     9001 ttccgaacat ccagaccagc cctttgcagc agttgcacct agaagttctg ctgaagaata
     9061 tggattggaa ctgattgccg aggatattca ggaaatggaa gccaatttca cacgtttctg
     9121 ggttctagga gctgaaaagc ctagtattcc cttgcaagca caaactgaaa agatgagttt
     9181 ggccttgaca ttacctgaca accttccagg tgcactttat aaggccctgt cgacctttgc
     9241 ttggcgagga attgacttga caaaaattga aagtcgtcca ctcaagacag cactgggtga
     9301 atactttttc cttatcgatg tggattatat cgataaggac ttggtccact ttgcccaaaa
     9361 agaattagaa gcgattggaa tccagtataa aattctgggt gcctatccta tttatccaat
     9421 atcagaccat ggaaaggaga gaagatgagt aaagaaaatc ccttaagtca tcatgagcag
     9481 ttgcgttatg attatttgct aaaaaatatt cactatctca atgagagaga aaaaaatgag
     9541 tttgcctatt tgcaagaaaa gctaactctt gctaggggaa atagtagctc tagcttggaa
     9601 caagaaagag aagagcaggt tgacttacca agctatgcga accggagtcg ctcacaatcc
     9661 aaatcacaag cactctcttt ccctccaaaa aagaaaagac ggaagctccg tcttaaacga
     9721 atttttatgg tgattttttc acttttggtc tgtgtggctt tggccatggt attcatgttt
     9781 ttgcgtggtt accaagatgc tagcgcaaag aaaactgctg atgcccgggc agctcaagta
     9841 gaagtcttta atggtcagga cactagagat ggagttaata ttttaatcat gggtactgat
     9901 ggtcgaatcg gccagaacag tgttgagaca cgaactgact ctattatggt attaaatgtc
     9961 gggggctcag ataagaaaat gaagctagtc agtttcatgc gtgataattt ggtctatata
    10021 gatggttata gtcaagtgat taatggtaga aaacagacag ataacaagtt aaacgtagcc
    10081 tacgagttag gagaacaaga ggggcaaaaa ggggcagaaa tggttcgcca agtcttgaaa
    10141 gataattttg acttggacat taagtactac gccttggttg atttccaggc ctttgccaca
    10201 gcgattgaca cgcttttccc tgatggggtg acgattgatg ctcaattttc aacattgaat
    10261 gggcgtcccc taacagaagc tacagtcgga gatgatttat atgctaccga gactgagtct
    10321 ccaacccaaa ccattaaagt cggaaaacag cagatgaatg gctcgaccct gctcaattat
    10381 gctcgtttcc gtgatgatga tgaggcggat tacggccgta caaaaagaca gcaacaagtt
    10441 ttaacagcaa ttttagagca aattaaagat cccactaaac ttttcactgg ttctgaagct
    10501 cttggaaagg tttttgctat gacctcaacc aacgtacctt atactttcct cctaacaaac
    10561 ggtttatctg ttttggatgg agcaaaaaat ggtattgaaa aattgacgat tccagaactg
    10621 ggtgactggg tagatgccta tgatgtttat ggaggcttgg gcttgctggt tgatcaaaac
    10681 aaatatcaaa ccaagctcgc tcaaatgggc ttgagataag ataatactca atgaaaatca
    10741 aagtgcaaac taggaagcta gccgcaggct gctcaaaaca cagttttgag gttgtagata
    10801 agactgacga agtcagttac ctatacctac ggcaaggtga agctgacgtg gtttgaagag
    10861 attttcgaag agtataagac agaaaaatca aagtttgata gctatttgga tagcagtcag
    10921 gctttgattt tttacagtct gcaatagatt ttcacccctt gtttgtggta taatgaaacg
    10981 atacgataga aagaataatg aacaatatga ctgatttaaa agcaattcag gatcgtagtc
    11041 tggagatggc tgaatatttt gtggcctttt gcaaggaaca tgatttactt tgctatctct
    11101 gtgggggagg cgctattggt gcccttcgaa acaaggggtt cattccttgg gacgacgacc
    11161 tagacttttt tatgcctcgt aaagattatg agaaattagc agaattatgg cctcgttatg
    11221 cagatgaacg ttatttcttg tcaaagagtt acaaggattt tgttgatcgt aatcttttta
    11281 ttaccattcg tgacaaggaa accacctgta tcaagcctta tcagcaggat ttggatttgc
    11341 cacatggtct ggccttggat gttttgcctt tggattatta tccgaaaaat ccagctgagc
    11401 ggaaaaaaca ggttcgttgg gccttgattt attcactctt ttgtgcgcaa actattccag
    11461 aaaagcatgg tgctctcatg aaatggggaa gtcgcatttt actgggtttg actccaaaat
    11521 ctctccgtta tcgcatctgg aaaaaagctg agaaagaaat gactaagtat gatttggctg
    11581 attgtgatgg cattacagaa ttatgctcag gtcctggcta catgagaaac aagtacccaa
    11641 tcacatcttt tgaagacaat cttttcttgc catttgaagg aacagagatg cctattccaa
    11701 tcggctatga tgtctatctc agaactgctt ttggggatta tatgacgcct ccaccagcag
    11761 acaagcaggt accgcatcat gatactgtca ttgctgatat ggataagtct tatacagaat
    11821 acaagggaga atatggtggc taagaaaaaa atcttatttt ttatgtggtc tttttctctt
    11881 ggaggtggtg cagagaagat tctatcaacc attgtttcaa atctggatcc agaaaagtat
    11941 gatattgata ttcttgaaat ggagcacttt gacaagggat atgaatctgt tccaaagcat
    12001 gtacgcattt taaaatccct tcaagattat cgccaaacca gatggttacg agcttttttg
    12061 tggagaatga gaatttattt tccaagactg gctcgtcgtt tgcttgtaaa agatgattat
    12121 gatgttgaag tttcttttac cattatgaat ccaccactgt tgttctctaa aagaagagaa
    12181 gtcaagaaga tatcttggat tcatggaagt attgaagaac ttcttaagga tagctctaaa
    12241 agagaatcac atagaagcca gttggatgct gcgaatacaa ttgtagggat ttcaaaaaag
    12301 accagcaatt ctatcaagga agtttatcca gattatgctt ctaaattaca gacaatctac
    12361 aatggatatg attttcagac tattctagaa aaatctcaag agaagatcga tatcgagatt
    12421 gctcctcaaa gtatctgtac tatcggacgg attgaggaaa ataagggttc tgaccgtgta
    12481 gtggaagtga tacgattatt acaccaagag ggaaaaaact atcatctcta ttttatcggg
    12541 gctggtgata tggaagagga actgaaaaaa cgagtcaaag agtatgggat tgaggactat
    12601 gtacatttcc ttggttatca aaaaaatcct tatcagtatt tatctcagac gaaagttctc
    12661 ttgtctatgt ctaaacaaga aggttttcct ggagtgtatg tggaggcctt gagtctggga
    12721 ctccctttta tctctacgga cgttggaggg gctgaggaat tatcccaaga aggacgattt
    12781 ggacaaatca ttgagagcaa tcaagaggca gctcaggcga ttactaatta catgacttct
    12841 gcctcaaact ttgatgtcga tgaggctagc caattcattc aacaatttac aattacaaaa
    12901 caaatcgaac aagtagaaaa actattagag gagtagcatg gaaactgcat taattagtgt
    12961 gattgtgcca gtctataatg tggcgcagta cctagaaaaa tcgatagctt ccattcagaa
    13021 gcagacctat caaaatctgg aaattattct tgttgatgat ggtgcaacag atgaaagtgg
    13081 tcgcttgtgt gattcaatcg ctgaacaaga tgacagggtg tcagtgcttc ataaaaagaa
    13141 cgaaggattg tcgcaagcac gaaatgatgg gatgaagcag gctcacgggg attatctgat
    13201 ttttattgac tcagatgatt atatccatcc agaaatgatt cagagcttat atgagcaatt
    13261 agttcaagaa gatgcggatg tttcgagctg tggtgtcatg aatgtctatg ctaatgatga
    13321 aagcccacag tcagccaatc aggatgacta ttttgtctgt gattctcaaa catttctaaa
    13381 ggaatacctc ataggtgaaa aaatacctgg gacgatttgc aataagctaa tcaagagaca
    13441 gattgcaact gacctatcct ttcctaaggg gttgatttac gaagatgcct attaccattt
    13501 tgatttaatc aagttggcca agaagtatgt ggttaatact aaaccctatt attactattt
    13561 tcatagaggg gatagtatta cgaccaaacc ctatgcagag aaggatttag cctatattga
    13621 tatctaccaa aagttttata atgaagttgt gaaaaactat cctgacttga aagaggtcgc
    13681 ttttttcaga ttggcctatg cccacttctt tattctggat aagatgttgc tagatgatca
    13741 gtataaacag tttgaagcct attctcagat tcatcgtttt ttaaaaggcc atgcctttgc
    13801 tatttctagg aatccaattt tccgtaaggg gagaagaatt agtgctttgg ccctattcat
    13861 aaatatttcc ttatatcgat tcttattact gaaaaatatt gaaaaatcta aaaaattaca
    13921 ttagaacggg ggtgaccaag tgattgatgg gaaacgatta ttatttagtt tgaccatagt
    13981 cagttatgcc ttgacgctag taagtggagt tgtgtatctg tttaataata ataatgttag
    14041 cttactttct actttattgt tcttgttggt tagtagctta attgcttgtt ggaatgatat
    14101 caagtattac ttaatccatt ttattttcta tttaaccatt tttgtatttc tggtatcaag
    14161 accgaccatt gattatttta gggatggtgc tttggatacc tatcatccaa tagcctatcg
    14221 ttttgccttt atagttgtca tggtttcgat tctgggcttg accacaggag gcattctggc
    14281 tcgttacttc atagctagga agaaaataaa agtagcaaat ataggaaatt ctctaaaaga
    14341 ggtttatatc aagcggttac gctttgtatc actaggagtt tttcttctaa cctatccttt
    14401 ctatttcatt cggttatttg aacggctctt gtatcgtttg cagacttcct actatgccta
    14461 ctatgcaaat tttgaaagta aactgcctta ttttacctac attttgtcta cctttacggt
    14521 ctatgcaatg tgtatgtatc tagcaaccaa gccaaagaaa ttgcaggcca cagcagtgct
    14581 tgtctccttt attgcagcta atactattca tttggcaatt gggacacgaa atccctttat
    14641 tttaagtatt ttatttgctt ttgtttatta ctttatgcgg gagcaaactg aaaaaggaaa
    14701 atggattggg tttaaagaaa agttagcgat ttttgtaggt tctcctattc tcatgttagc
    14761 gatgggagta ctcaattatg tacgggataa tgtccaagtt tcccatacag gtttctggga
    14821 tatcttactt gactttatct ataaacaagg gactagtttt ggtgttctgg ctcgaggttt
    14881 tctatttaac agtagcctcc cttaccgaga tttccgtaat tttacttttg gtcctgttct
    14941 tgattatttt gcaaggggga gtttgggagc cattttcgga ggaaaagcct ttgaacatac
    15001 aaccaatagt gtggaactag ctattgatag taatagttat gcccacaatc tatcctatct
    15061 tgtcttgaac aaggaatact tgaaagggca tggtatcgga agtagttata tcatggagtt
    15121 gtataccgac tatggtatga ttggagtctt tctgcttagt ttcttactcg gcgtattatt
    15181 tatagccatg ctgcaagtag cctatcgctc aaggacaatc ctatttgctt tatccctact
    15241 catcttgaat aatctattct ttatgccaag aagcagcttt tcagaaagtt tcttcaattt
    15301 atttacaatg caattctggg gaattgttct tgtgattata tttgtagcaa aaatgcttac
    15361 aaaggaaaac tagtatctac taaacaaagg agaaaaaaat catgtttgaa cattattcag
    15421 tagctgattt gtttgcaaat ctttataaaa agcgaaaagc aaatatttta gctctcatcg
    15481 ctttatttgc tctcattgct gtaccattta caattaaagc agttaggaat aaaaacactg
    15541 tcaaagatac aacaagttat tcaacttatc ttatctataa aatcactcct ccaaaagagt
    15601 cggccaaaac gattttgaat catcaaattg gtggttatag tgatttttat gggaaattga
    15661 ttgatggtaa tttgaatgga gcttatcttt tcaatgatgt agaacccagt gagttgaaaa
    15721 aaattgccag tgaattagac acgacagaaa caaccttgaa aaattcaacg aatgactatt
    15781 ggtggaaaaa attgaccgtc tactatatga ttgacgatgc aggggttggt gtgaaaattt
    15841 tgacatcaag taaagatgcc aatgacttgt tagagaaaaa aattgatggg ttgattgaga
    15901 aatttaaaca tgcttatgca aatgtgaaaa ttgaaaaact ggaaaccatc aactctaaag
    15961 aattgaacgc aaatggtgaa acagcgcttg gcttaaatgt gaaaaatctg attcttcgtt
    16021 tagttgttat tggagtggtt tgtgtgattt tggttgtgat gggaaatgtg ttagtttatc
    16081 tctttaatcc aacaatcaat agagtaggtg atttttctca gtatcaaatt gattttgtaa
    16141 cagagattac aacaattgct aacctagcag atgttttgtc atacaaaaat actggacagg
    16201 aattgaccat cgttagctca aataaagcta tcctagataa attgaaacag agtcaagaag
    16261 ctttaaaagg aatgcatttt gtagatttac aggatgtatc atctcttttg gaaagagata
    16321 cagtccttct tgttgaagag tacggagtga ctcgttataa gaaatttgag caaagtcttc
    16381 aaattctcag aaacttaaat cgttctatcc tcggtgtagc aacctttaaa ttataagtat
    16441 tctgatgtat aaggtcttca aagtcggcta agagccgatt ttgaaggctt tttctatctg
    16501 tctgtctgca gaaattttct tttgataaaa tcgtaaaaat ctagtataat agatagaact
    16561 gaaagtatga ggttactagc aatgaaaatg aaacaaatta gtgatacaac tttaaaaatc
    16621 acgatatctt tagaggattt gatggatcgt ggaatggaga ttgctgactt tctcgttcct
    16681 caagaaaaaa cagaagagtt cttttatgct atcttggatg agctagagat gcctgatagc
    16741 tttctggata caggcatgtt gagcttccgt gtgactccaa aacctgataa ggtagatgtc
    16801 tttgtgacca agtcaaagat tgaccaaaat ctagattttg aagacttatc ggatttgcca
    16861 gatatggaag aattggctca aatgtctcca gatgaattta tcaaaaccct ggaaaaaagc
    16921 atcgcagaca aaaccaagga tgatatcgaa gccattcaat ctcttgagca agttgaagcc
    16981 aaggaagaag agcaagagca ggctgaacaa gaagctgaga gtaagaaaga accttacatc
    17041 tactacatcc tttcttttgc taagttggct gacttggtag cttttgccaa gacagtgact
    17101 tttgaaatgg aaacttctga actctacaaa atgaacgagc gctattattt gaccatttta
    17161 gtggatattg aaaatcatcc aagcccatat ccagcttggc tgttggcccg tatgcgcgag
    17221 tttgcagagg atagtgatat cagtcgctca gtcttacaag agtatggtca agtcttgatg
    17281 agtcacgatg cagtgctcaa tctgcaaaaa atcggctaat cctttctgga aagctatttt
    17341 cagattttaa gaagagcgaa tcgtctgatt cgttttttct ttttatactg aaatagtgat
    17401 ttactataat aggaattttc acaaaattct gttataatgg ctatattaga aaatttcgag
    17461 gagataaaga tgacagttaa aattgcttta ctgggatttg gtaccgttgc aagtggtgtg
    17521 cctttcctcc taaaggaaaa tggaggaaaa atcaatcaat cagcacattc agatatcaaa
    17581 gttgctaagg tattggtcaa ggatgaagat gaaaaaaatc gcttgcttgc agcagggaat
    17641 gactttaact ttgtaaccaa tgtggatgat attttatcag accaggatat tactatcgta
    17701 gtggaattga tggggcgtat tgagcctgct aaaaccttta tcactcgtgc cttggaagct
    17761 ggaaaacacg ttgttactgc taacaaggac cttttagctg tccatggcgc agaattgcta
    17821 gaaatcgctc aagctaacaa ggtagcactt tactacgaag cagcagttgc tggtgggatt
    17881 ccaattcttc gtactttagc aaattccttg gcttctgata aaattacgcg cgtgcttgga
    17941 gtagtcaacg gaacttccaa cttcatggtg accaagatgg tggaagaagg ctggtcttac
    18001 gatgatgctc ttgcggaagc acaacgtcta ggatttgcag aaagcgatcc gacgaatgac
    18061 gtagatggga ttgatgcagc ctacaagatg gttattttga gccaatttgc ctttggcatg
    18121 aagattgcct ttgatgatgt agcccacaag ggaatccgca atatcacacc agaagacgta
    18181 gctgtagctc aagagcttgg ttacgtagtg aaattggttg gttctattga ggaaacttct
    18241 tcaggtattg ctgcagaagt gactccaacc ttcctaccta aagcgcaccc acttgctagt
    18301 gtgaatggcg taatgaacgc tgtctttgta gaatctatcg gtattggtga gtctatgtac
    18361 tacggaccag gtgcgggtca aaaaccaact gcaacaagtg ttgtagctga tattgtccgt
    18421 atcgttcgtc gtttgaatga tggtactatt ggcaaagact tcaacgaata tagccgtgac
    18481 ttggtcttgg caaatcctga agatgtcaaa gcaaactact atttctcaat cttggctcca
    18541 gactcaaaag gtcaggtctt gaagttggct gaaatcttca atgctcaaga tatttccttt
    18601 aagcaaatcc ttcaagatgg caaagagggt gacaaggcgc gtgtcgttat catcacacac
    18661 aagattaata aagcccagct tgaaaatgtc tcagctgaat tgaagaaggt ttcagaattc
    18721 gacctcttga ataccttcaa ggtgctagga gaataagatg aagattattg tacctgcaac
    18781 cagtgccaat atcgggccag gttttgactc ggtcggtgta gctgtaacca agtatcttca
    18841 aattgaggtc tgcgaagaac gagatgagtg gctgattgaa caccagattg gcaaatggat
    18901 tccacatgat gagcgtaatc tcttgctcaa aatcgctttg caaattgtac cagacttgca
    18961 accaagacgc ttgaaaatga ccagtgatgt ccctttggcg cgcggtttgg gttcttccag
    19021 ctcggttatc gttgctggga ttgaactagc caaccaactg ggtcaactca acttatcaga
    19081 ccatgaaaaa ttgcagttag cgaccaagat tgaagggcat cctgacaatg tggctccagc
    19141 catttatggt aatctcgtta ttgcaagttc tgttgaaggg caagtctctg ctatcgtagc
    19201 agactttcca gagtgtgatt ttctagctta cattccaaac tatgaattac gtactcgcga
    19261 cagccgtagt gtcttgccta aaaaattgtc ttataaggaa gctgttgctg caagttctat
    19321 cgccaatgta gcggttgctg ccttgttggc aggagacatg gtgaccgctg ggcaagcaat
    19381 cgagggagac ctcttccatg agcgctatcg tcaggacttg gtaagagaat ttgcgatgat
    19441 taagcaagtg accaaagaaa atggggccta tgcaacctac ctttctggtg ctgggccgac
    19501 agttatggtt ctggcttctc atgacaagat gccaacaatt aaggcagaat tggaaaagca
    19561 acctttcaaa ggaaaactgc atgacttgag agttgatacc caaggtgtcc gtgtagaagc
    19621 aaaataaaga atagaagata ggatggggaa actcttgacc agagggtttc atatcctttt
    19681 tgtgaaaaga agtttatact caatgaaaat caaagagcaa actaggaagc tagccgcagg
    19741 ctgctcaaga cactgttttg aggttgcaga tagaactgac gaagtcaact caagacactg
    19801 ttttgaggtt gcagatagaa ctgacgaagt cagctcaaga cactgttttg aggttgcaga
    19861 tagaactgac gaagtcagta accataccta cggtaaggtg acgctgacgt ggtttgaaga
    19921 gattttcgaa gagtattagt taaaaacgtg ataaaggaga aataaaaatg gcagaaattt
    19981 atctagcagg tggttgtttt tggggcctag aggaatattt ttcacgcatt tctggagtgc
    20041 tagaaaccag tgttggctac gctaatggtc aagtcgaaac gaccaattac cagttgctca
    20101 aggaaacaga ccatgcagaa acggtccaag tgatttacga tgagaaggaa gtgtcactca
    20161 gagagatttt actttattat ttccgagtta tcgatcctct atctatcaat caacaaggga
    20221 atgaccgtgg tcgccaatat cgaactggga tttattatca ggatgaagca gatttgccag
    20281 ctatctacac agtggtgcag gagcaggaac gcatgctggg tcgaaagatt gcagtagaag
    20341 tggagcaatt acgccactac attctggctg aagactacca ccaagactat ctcaggaaga
    20401 atccttcagg ttactgtcat atcgatgtga ccgatgctga taagccattg attgatgcag
    20461 caaactatga aaagcctagt caagaggtgt tgaaggccag tctatctgaa gagtcttatc
    20521 gtgtcacaca agaagctgct acagaggctc catttaccaa tgcctatgac caaacctttg
    20581 aagaggggat ttatgtagat attacgacag gtgagccact cttttttgcc aaggataagt
    20641 ttgcttcagg ttgtggttgg ccaagtttta gccgtccgat ttccaaagag ttgattcatt
    20701 attacaagga tctgagccat ggaatggagc gaattgaagt tcgttctcgt tcaggcagtg
    20761 ctcacttggg tcatgttttc acagatggac cgcgggagtt aggcggcctc cgttactgta
    20821 tcaattctgc ttctttacgc tttgtggcca aggatgagat ggaaaaagca ggatatggct
    20881 atctattgcc ttacttaaac aaataaaaca gagagtgggg cttcccactt tcttcatttc
    20941 tagaatatga atagaaggga tttatgaaac acctattatc ttacttcaaa ccctacatca
    21001 aggaatcaat tttagccccc ttgttcaagc tgttagaagc tgtttttgag ctcttggttc
    21061 ccatggtgat tgctgggatt gttgaccaat ctttacctca gggagatcaa ggtcatctct
    21121 ggatgcagat tggcctgctc cttatctttg cagtaattgg cgttttagtg gccttgatag
    21181 ctcaatttta ctcagcaaag gcagcagtag gttttgctaa ggaattgaca aacgatcttt
    21241 atcgtcatat tctttccttg cccaaggaca gcagagaccg tctgacaact tctagtttgg
    21301 ttactcgctt gacttcggat acctaccaga ttcagactgg tatcaatcaa ttcctgcgtc
    21361 tctttttacg agcgcccatt atcgtttttg gtgccatttt tatggcttat cgaatctcag
    21421 ctgagttgac tttctggttc ttagtcatgg ttgccatttt gaccattgtc attgtagggt
    21481 tatctcgatt ggtcaatcct ctctacagta gtctcagaaa gaaaacggac caactggttc
    21541 aggaaacgcg ccagcaattg caagggatgc gggttattcg tgcttttggt caagaaaaac
    21601 gagagttaca gatttttcaa acccttaacc aagtttatgc tagattacaa gaaaagacag
    21661 gtttcttgtc tagtttatta acacctctga cctatctgat tgtcaatgga actcttctcg
    21721 ttattatctg gcaaggctat atttcaattc aaggaggagt actcagtcaa ggtgctctca
    21781 ttgctcttat caattacctc ttacagattt tggtggaatt ggtcaagcta gccatgctga
    21841 tcaattccct caaccagtcc tatatctcag ccaagagaat cgaggaggtc tttgcggaat
    21901 ctcccgaaaa catccattca gaattagaac aaaagcaagt taccagtggt cgggttttac
    21961 aagtccaaga attgactttt acctatcctg atgcggccca gccttctctg agagacattt
    22021 cctttgatat gactcaagga caaatccttg gtatcattgg ggggactggt tctggtaaat
    22081 caagcttggt gcaactctta cttggacttt atccagtaga caaggggaac attgaccttt
    22141 atcaaaatgg acgtagtcct cttaatttgg agcagtggcg gtcttggatt gcctatgtac
    22201 ctcaaaaggt cgaactcttt aagggaacta ttcgttccaa cttgacttta ggtttaaatc
    22261 aagaagtgac tgatcaagaa ctctggcaag ccttggagat tgcgcaagct aaggattttg
    22321 tcagtgataa ggaaggactc ttggatgctc tagttgaagc aggagggcga aatttctcag
    22381 gtggacaaaa acaaaggctg tctatcgcac gagcagtctt gcgccaggct ccgtttctca
    22441 tcctagatga tgcgacctca gcactggaca ccattacaga gtccaagctc ttgaaagcta
    22501 ttagagaaaa ttttccaaac atgagcttaa ttttgatctc tcaacgaacc tcgactttac
    22561 agatggcgga ccagattctc ctcttggaaa aaggtgagtt gctagctgtt ggcaagcacg
    22621 atgacttgat gaaatccagt caagtctatc gtgaaatcaa tgcatcccaa catggaaagg
    22681 aggactagaa tgaaacgaca aactgtaaac cagacgctca aacgtttagt cgtagattta
    22741 gcaaaccatc ccttcctcct tttcctagcc tttctaggaa ctattgccca agttggctta
    22801 tcaatttacc tacctattct gattgggcag gtcattgacc aagtcctagt ggctggttca
    22861 tcaccagttt tttggcagat ttttctccag atgctcttgg tggtaatagg aaatactctg
    22921 gtacaatggg ccaatcctct cctctataat cgtctaatct tctcttatac cagagattta
    22981 cgagagcgaa tcatccataa actccatcgg ttaccgattg cttttgcgga tcggcaaggc
    23041 aatggagaga tggtcagtcg tgtaaccacg gatatcgaac agttggcagc tggcttgacc
    23101 atgattttta accaattttt cattggtgtt ttgatgattt tggttagtat tctagccatg
    23161 ctccaaattc atctcctcat gactctctta gtcttgctgt tgacgccact gtccatggtg
    23221 atttcacgct ttattgccaa gagatcctat catctcttcc agaagcaaac agagacgagg
    23281 ggaattcaga ctcagttgat tgaagaatcg cttagccagc agactataat ccagtccttc
    23341 aatgctcaaa cagaatttat ccaaagattg cgtgaggctc atgacaacta ctcaggctat
    23401 tctcagtcag ccatctttta ttcttcaacg gtcaatcctt cgactcgctt tgtaaatgca
    23461 ctcatttatg cccttttagc tggagtagga gcttatcgta tcatgatggg ttcaaccttg
    23521 accgtcggtc gtttagtgac ttttttgaac tatgttcagc aatacaccaa gccctttaac
    23581 gatatttctt cagtgctagc tgagttgcaa agtgctctgg cttgcgtaga gcgtatctat
    23641 ggggtcttag atagccctga agtggctgaa acaggtaagg aagtcttgac cagtgaccaa
    23701 gttaagggag ctatttcctt taaacatgtc tcttttggct accatcctga aaaaattttg
    23761 attaaggagt tgtctatcga tattccagct ggtagtaagg tagccatcgt tggtccgaca
    23821 ggtgctggaa aatcaactct tatcaatctc cttatgcgtt tttatcccat tagctcggga
    23881 gatatcttgc tggatgggca atccatttat gattatacac gagtatcatt gagacagcag
    23941 tttggtatgg tgcttcaaga aacctggctc acacaaggga ccattcatga taatattgcc
    24001 tttggcaatc ctgaagccag tcgagagcaa gtaattgctg ctgccaaagc agctaatgca
    24061 gactttttca tccaacagtt gccacaggga tacgatacca agttggaaaa tgctggagaa
    24121 tctctctctg tcggccaagc tcagctcttg accatagccc gagtctttct ggctattcca
    24181 aagattctta tcttagacga ggcaacttct tccattgata cacggacaga agtgctggta
    24241 caggatgcct ttgcaaaact catgaagggc cgcacaagct tcatcattgc tcaccgtttg
    24301 tcaaccattc aggatgcgga tttaattctt gtcttagtag atggtgatat tgttgaatat
    24361 ggtaaccatc aagaactcat ggatagaaag ggtaagtatt accaaatgca aaaagctgcg
    24421 gcttttagtt ctgaataagc cattctcttt tgaaagttta tggacgaaaa aagttgcctt
    24481 cgagtgactt ttttgttaca atagctagaa aaattgttca ctgtaatact caatgaaaat
    24541 caaagagcaa actaggaagc tagccgtagg ttgctcaaag cacagctttg aggttgtaga
    24601 taagactgac gaagtcagct caaaacactg ttttgaggtt gcagatagaa ctgacgaagt
    24661 cagctcaaaa cactgttttg aggttgcaga tagaactgac gaagtcagta accacaccta
    24721 cggcaaagtg aatttgaagt ggtttgaaga gagtacaact tgtcttttag aaaaggagcc
    24781 tataatgaaa gtctttcagc atgtaaatat cgtgacttgt gatcaagatt tccatgttta
    24841 tcttgatgga atcttagcag tcaaggattc tcaaatcgtc tatgtcggtc aagataagcc
    24901 agcgttttta gaacaagctg agcagattat agactatcag ggagcttgga ttatgcctgg
    24961 tttggtcaat tgtcacaccc attctgcaat gacaggtctg agagggatcc gagatgacag
    25021 caatctccat gaatggctca atgactatat ctggccagca gaatctgagt ttactcccga
    25081 catgactacc aatgcggtca aagaagcctt gacagagatg ctccagtcag gaacaacaac
    25141 ctttaacgat atgtataatc ccagtggtgt ggatatccag caaatttatc aggtggtgaa
    25201 aacttccaag atgcgttgtt atttttctcc gactctcttt tcttcagaga cagaaacaac
    25261 tgctgagact ataagcagaa ctcgatccat catagatgaa atcttaaaat ataaaaatcc
    25321 aaatttcaag gttatggtag cacctcattc tccgtatagc tgcagtagag acttgctgga
    25381 agcgagtttg gaaatggcaa aagagctaaa tattccgctc catgtccatg tggcggagac
    25441 caaggaggag tcaggaatta tcctcaaacg atacggcaaa cgcccccttg cttttcttga
    25501 agaactgggt tatttagatc atccgtccgt atttgctcac ggggtcgaat taaacgagag
    25561 agaaattgaa cgcttggcat cttctcaagt ggctatcgcc cacaatccta tcagtaacct
    25621 caaactggca tcaggaattg ctccaattat ccagctccaa aaagcgggag tagcagtcgg
    25681 aattgcgact gactcggttg cttccaataa caatctagat atgtttgagg aaggaaggac
    25741 tgcagctctt cttcagaaga tgaaaagtgg ggatgccagc cagtttccaa tcgaaacagc
    25801 tctcaaggta ctgacaatcg aaggggctaa ggtccttgga atggaaaatc agataggaag
    25861 tctggaagtc ggcaagcaag cagattttct ggtcattcaa ccacaaggga aaattcatct
    25921 ccaacctcag gaaaatatgc tgtctcacct ggtttatgca gttaaatcta atgatgtaga
    25981 tgatgtttat atcgccggag aacaggttgt taagcaaggt caagtcctga cagtagaact
    26041 ttaaaagaaa aatcacgaaa aattttaaaa aaagtttgca aaaatcttgc attctttttt
    26101 tgactatgct atacttatat acggtttaaa aaaactgcct aagacagtag gggagctcga
    26161 ctcataaata tcctaccgag gacaagacgt atcatgtaaa aagaagcgta ttgtactttc
    26221 gtgtctaggt ttgggcgcgt ttttcttttt gaaaaattcc ccaagcaaaa taattacgga
    26281 ggtgaacaca ctaatgagtg aagcaattat tgctaaaaaa gcggaactag ttgacgtagt
    26341 agctgaaaaa atgaaagctg ctgcatctat cgtcgttgta gacgctcgtg gtttgacagt
    26401 tgagcaagat acagttcttc gtcgtgagct tcgtggaagc gaagttgagt ataaagttat
    26461 taaaaactca atcttgcgtc gtgcagctga aaaagctggt cttgaagatc ttgcatctgt
    26521 atttgttgga ccatctgcag tagcattttc taatgaagat gttatcgcac cagcgaaaat
    26581 cttgaacgac ttttctaaaa acgctgaagc acttgaaatt aaaggtggtg caatcgaagg
    26641 cgctgtcgca tctaaagaag agattcttgc acttgcaact cttccaaacc gcgaaggact
    26701 tctttctatg ctcctttctg tacttcaagc gccagtgcgc aacgttgctc ttgcagtcaa
    26761 agcggttgca gaaagcaaag aagacgcggc ttaatcttaa gctacacagc gtagcctagc
    26821 tacgaaaaaa actattataa aatttaaaac ttatttggag gaaataacaa tggcattgaa
    26881 cattgaaaac attattgctg aaattaaaga agcttcaatc cttgaattga acgaccttgt
    26941 aaaagctatc gaagaagaat ttggtgtaac tgcagctgct cctgtagctg ttgctgcagc
    27001 tgatgcagct gatgctggtg ctgctaaaga ttcattcgac gttgaattga catctgcagg
    27061 cgacaaaaaa gttggcgtta tcaaagttgt acgtgaaatc actggtcttg gtcttaaaga
    27121 agctaaagaa cttgttgacg gtgcaccagc acttgttaaa gaaggcgttg caactgcaga
    27181 agctgaagaa atcaaagcta aattggaaga agctggagct tcagttactc ttaaataata
    27241 gagtatcgca atttgattgc ggaatcccta tttaccagtc tttgagagcg ataagcgatt
    27301 gatagaaact gataaattga tttggtttcc tgccaaaaat tctttggcag gatttttgtt
    27361 ttaataaact ttcggggggt agacactaga ttgtctaccc ctttttttgt gtttcggagg
    27421 taattatgca aaaggaagga cagtcatcac gtcaattcgt gaagagtcat ctcatgacta
    27481 tcatgggagt tgatattgta aaagcaactc agttagttga tgaaatggaa caagtaggtt
    27541 taatccgatt tgatgaattt ggaaatgttg gaattttagt cttggaggga caataatgaa
    27601 gcgtattacc gctaatcaat atcagacatc agagcgttat tataagctcc ccaaactttt
    27661 atttgagagt gaacggtata agaatatgaa actggaggtt aaggtggttt actcagtttt
    27721 aaaagatcgg ttggagttgt ctttgagcaa aggttggatt gatgaggatg gggctattta
    27781 tttgatttat tccaattcaa atttgatggc acttttaggc tgttcaaagt caaaattact
    27841 ctccatgtga gtttgaagta acatttttag atgattacca taaaaaacac aactacccac
    27901 tattttacga gtcctatctt caaaacgtta tggaattcct tgaaagtcaa gacataaaga
    27961 atggggttga tgcctttgta gatgatcatc aaaatctcgt ttttgtttta tatggacaag
    28021 gctatcgagc cgagggaaaa gagggaatac ttacaaccca agtaactgta aaagcttatg
    28081 atgaagacaa gaaaccgatt aacttcgcaa atttattaga ttccttaatc gtctcagaat
    28141 atcaaatgga accgaatctt tgggaggtct cccatgattg atctctatct aagtaaaaat
    28201 agacaaagaa atcaacttct tttagatttc ttccaaaact atggcataga ggtatcttgt
    28261 cattcagttt ctgaaatgac aaaggacaaa ttaattgaga tgatgagcta ttcttcagat
    28321 tgttttgagt ttttatctcc caatttatta cgatttaaga accgtgacaa tctaagatta
    28381 acggatttcg ttgagataat tttaaaaaat cctgaactaa ccatcagact tcctcttgcg
    28441 gtttcaaata agcgagttta tccaagtctg aatttggaag aggctagagc tctattgcca
    28501 agagatacga aacaattgat ttacatggcg caaacacatt atttatctaa ctaaaggaga
    28561 agcaatatgg ctgaacgatt ttgggagaac ttgtccatta ttttggctga aagaaatatc
    28621 agctgtattg agttaaccag aaaaatgttt gcgggagagt ttcactatcc aagtgaattg
    28681 aatcgtttgt atcaaaagat tcgccactag aagatggaac aacgaatgcc tcaaagttca
    28741 tgggtagaaa ggattgtgca ggtattagat ttagattatg aagatttatt tcggaggtaa
    28801 ctatgaaaag aataattcca gtttatatat tccaacaagt aaatgtccta ttggtatctc
    28861 tatacttatt gaaatttctt tgtatcggtg agttaactat actacagatt ctctatggtt
    28921 cgtcactcat ttctttttta tggatgtatg gccaacgcaa acaagctcat aaggtaaata
    28981 tgaaatctag gatgaaatgg cttggtattg gattcgttag cctactgatt ataagtctat
    29041 gttttagtct aatccatgcg caagaaagca cgaatcaagc aaacttaatt gggcttcaac
    29101 atcaagttcc ttggttttca tttttattgt ttttaatcaa tgcgagtatg gttgaagaat
    29161 ttctgtatag agaaatttta tggaacttgg ttagaaaatt agatattcga gttgcgttga
    29221 caagtgtttt atttgcctta gcacatcatc caggaaccat tatagcttgg tgtttgtatg
    29281 tatcgctggg gatgttttta gggcttgtac gctacaaatt ggacttatgg ggcagtatgg
    29341 ggctacattt ggtgtggaac ctactagttt atagtttgct gcttttttaa aataggtgac
    29401 ctgattttta ggtaacttct aaaactaact tccagttttg ggagagagat ggaagttact
    29461 ttgaaaagtt acggaaacat tattttctat gtgacaacat tgtataattg cttgcatttt
    29521 actggtggag agagtataat gagggtggat aaagtactta atcccaaaaa tttaaatagg
    29581 aggagctagc tatggcagaa gaagtattaa atcttcaatt agtatctgtt caagttgacg
    29641 aaacagatga agttgatgga atgcgattta gtacattcag tacaaatcgt tgcggtaact
    29701 ggagtgcttt tagttgggaa aattgtt
//
LOCUS       DAVGJL010000011        29657 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.011, whole genome shotgun sequence.
ACCESSION   DAVGJL010000011 DAVGJL010000000
VERSION     DAVGJL010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29657
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.011"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            2..1279
                     /locus_tag="VVE42_000421"
     CDS             2..1279
                     /locus_tag="VVE42_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcysteine synthetase"
                     /protein_id="HEU3072679.1"
                     /translation="MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEV
                     IKDLFHYLVSTLDLTVAKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQE
                     VENRFNNYMNVIQRKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNI
                     IKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWD
                     TKISRDIFWEESMHGIYPENVGVNARLLNDEADFFDYLNHSAIFTAERDGQTYYFYPI
                     QAGDYLATPEIQAFALNGDEVIIYPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLD
                     RTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKFLRRQFSKKNLTDEEETMIIE
                     FSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL"
     gene            1355..2839
                     /locus_tag="VVE42_000422"
     CDS             1355..2839
                     /locus_tag="VVE42_000422"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEU3072680.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPVTYPKVDLNF
                     NKLKDASYQEVAKLVLSAFLDDFTAEELDYCINNAYDSKFDTPAIAPLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFADVPGT
                     EIIVFYPKDGVSKIQELQMTTQTGDNTHVIAIDGNFDDAQTNVKHMFNDVALREKLTT
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIVAGDKINFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNDAVKTAELMNALNSQGQYELTNFDAEILDLFAAEYATEEETAAEIKRVYESDS
                     YIEDPHTAVASAVYRKYQATTGDATKTVIASTASPYKFPVVAVEALTGKVGLTDFEAL
                     AQLHEISGVAVPPAVDGLETAPVRHKTIVAAADMQVAVEAYLGL"
     gene            2900..4180
                     /locus_tag="VVE42_000423"
     CDS             2900..4180
                     /locus_tag="VVE42_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEU3072681.1"
                     /translation="MFKKNKDILNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTILSRLVGLVILWSQLKLPYGKPTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIVLVVSFGTEAVAGNAIGEVLTQFNYMPAFGVATA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTPMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLMGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMSLKG"
     gene            4184..4804
                     /locus_tag="VVE42_000424"
     CDS             4184..4804
                     /locus_tag="VVE42_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU3072682.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFAQFSIPYDKEKVREF
                     IFKYSVQDLLVRVAEDRNLDVEVLNQVRAQSLAEKNAQVVLMPGAREVLAWADESGIQ
                     QFIYTHKGNNAFTILKDLGVESYFTEILTSQSGFVRKPSPEAATYLLDKYQLNSDNTY
                     YIGDRTLDVEFAQNSGIQSINFLESTYEGNHRIQALADISRIFETK"
     gene            4970..6112
                     /locus_tag="VVE42_000425"
     CDS             4970..6112
                     /locus_tag="VVE42_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU3072683.1"
                     /translation="MKKRMLLASTVALSFAPVLATQAEEVLWTARSVEQIQNDLTKMD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVTVDDQTVQVADLSQPIAEAPKEVASSSEVTKTV
                     IASEEVAPSTGTSVPEEQTTETTRPVEEATPQETTPAEKQETQASPQAASAVEVTTTS
                     SEAKEVASSNGATAAVSTYQPEETKIISTTYEAPAAPDYAGLAVAKSENAGLQPQTAA
                     FKEEIANLFGITSFSGYRPGDSGDHGKGLAIDFMVPERSELGDKIAEYAIQNMASRGI
                     SYIIWKQRFYAPFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG"
     gene            6518..6856
                     /locus_tag="VVE42_000426"
     CDS             6518..6856
                     /locus_tag="VVE42_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEU3072684.1"
                     /translation="MTRYQILLFLLEHSLCNQMAVQERLKIDQAALTRHFKILETEGL
                     VERHRNPENQREVLVEAAKYAKEQLVVNPPLQHIKVKEEMESILTEFERTELSRLLNK
                     LVLGIENIEI"
     gene            6867..7226
                     /locus_tag="VVE42_000427"
     CDS             6867..7226
                     /locus_tag="VVE42_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEU3072685.1"
                     /translation="MSIILTTIVALEHFYIFYLESIATQSDATSRVFNMEKEELAHPS
                     VSSLFKNQGIYKDLLGVFLLYGIYFSQNLEIVTIFVLFVIGAATYGSLTADKKIILKQ
                     GGSAILALISILLFKYT"
     gene            7333..7976
                     /gene="pcp"
                     /locus_tag="VVE42_000428"
                     /pseudo
     CDS             7333..7976
                     /gene="pcp"
                     /locus_tag="VVE42_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
     gene            complement(8004..8861)
                     /locus_tag="VVE42_000429"
     CDS             complement(8004..8861)
                     /locus_tag="VVE42_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEU3072686.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSSFLNP
                     LSRIHGTVLEQPFFSILGQILQAYLFPLLVSFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVP
                     PRRILKTSRSFMKGYKFQHFVLDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYRA
                     VLARKRPKA"
     gene            8978..10120
                     /gene="tgt"
                     /locus_tag="VVE42_000430"
     CDS             8978..10120
                     /gene="tgt"
                     /locus_tag="VVE42_000430"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEU3072687.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREYFVEKYGYNKSGRNF"
     gene            10218..12035
                     /locus_tag="VVE42_000431"
     CDS             10218..12035
                     /locus_tag="VVE42_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEU3072688.1"
                     /translation="MRIKWFSLIRIIGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNNEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     AGVLGFMTNFYELLTGGSYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQAKSN
                     GQLKGMVFLLSAAAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSMLATIVGV
                     RQTTSLVKQLDKIWDLRKTLVVFGGGFGFLVLLTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTHHIQEPKIISFLADTSYAVYLFHWPFYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIVGKNTPILQTLRPLPYIHAILAAGTGILTFIVCMVTLLAPQV
                     GAFETDLTVNGLKQAATNIGQTKVMAERADANSLGIADGTMLIGDSVALRANTALQTA
                     LPGAQINAQVSVTTKTANEIMLNNSQNKFLPKTVVIATGVNNPENYKDDWDSIVKNLP
                     KGHHMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITIADWNQVAKEHPEIWAG
                     TDQVHFGSESSTIEAGAKLYADTIATALQTAQDKPVKSK"
     gene            12188..13339
                     /gene="nagA"
                     /locus_tag="VVE42_000432"
     CDS             12188..13339
                     /gene="nagA"
                     /locus_tag="VVE42_000432"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEU3072689.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPYTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
     gene            13502..14560
                     /locus_tag="VVE42_000433"
     CDS             13502..14560
                     /locus_tag="VVE42_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEU3072690.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLIDGMQAEYLRVPHADNTLYHTPEDLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLDTAVSFGATHKVN
                     SSDPEKAIKEIYDLTDGRGVDVAIEAVGIPETFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            14711..15076
                     /locus_tag="VVE42_000434"
     CDS             14711..15076
                     /locus_tag="VVE42_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEU3072691.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            15152..16093
                     /locus_tag="VVE42_000435"
     CDS             15152..16093
                     /locus_tag="VVE42_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HEU3072692.1"
                     /translation="MVQEIAQEIIRSARKKGAQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDQKIASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAN
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            16041..17057
                     /locus_tag="VVE42_000436"
     CDS             16041..17057
                     /locus_tag="VVE42_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEU3072693.1"
                     /translation="MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSSLLDKQCVTQMRTGLSQGKSFSEMMESLGCSSTIVTQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
                     LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEIGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIVLLYAAMLMPMYQNM
                     EVNF"
     gene            17059..17385
                     /locus_tag="VVE42_000437"
     CDS             17059..17385
                     /locus_tag="VVE42_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEU3072694.1"
                     /translation="MKKMMTFLKKAKVKAFTLVEMLVVLLIISVLFLLFVPNLTKQKE
                     AVNDKGKAAVVKVVESQAELYSLDKNEDATLSKLQGDGRITEEQVKAYNEYYTKNGGA
                     NRKVND"
     gene            17378..17782
                     /locus_tag="VVE42_000438"
     CDS             17378..17782
                     /locus_tag="VVE42_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEU3072695.1"
                     /translation="MIKAFTMLESLLVLGLVSILALGLSSSVQSTFAAVEEQIFFMEF
                     EELYRETQKRSVASQQKTSLNLDGQTLSNGSQKLTVPKGIQAPSGQSITFDRAGGNSS
                     LAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            17787..18047
                     /locus_tag="VVE42_000439"
     CDS             17787..18047
                     /locus_tag="VVE42_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3072696.1"
                     /translation="MILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEVLRVA
                     KMALQTGQNQVSINGVEIQVFSSEKGLEVYHGSEQLLAIKEP"
     gene            18010..18471
                     /locus_tag="VVE42_000440"
     CDS             18010..18471
                     /locus_tag="VVE42_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEU3072697.1"
                     /translation="MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQTMSQLLI
                     SEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGNRLYMKQDGKDIAIGKSKSDDFR
                     KTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES"
     gene            18449..18841
                     /locus_tag="VVE42_000441"
     CDS             18449..18841
                     /locus_tag="VVE42_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEU3072698.1"
                     /translation="MWKKKKVKAGVLLYAVTIAAIFSLLLQFYLNRQIAHYQDYALNK
                     EKLVAFAMAKRTKDKAEQESGEQVFNLGQVSYQNKKTSLVTTVRTSKSQYEFLFPSVK
                     IKEEKRDKKEEVATDSSEKAEKKIRREA"
     gene            18997..19591
                     /locus_tag="VVE42_000442"
                     /pseudo
     CDS             18997..19591
                     /locus_tag="VVE42_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            19652..20605
                     /locus_tag="VVE42_000443"
     CDS             19652..20605
                     /locus_tag="VVE42_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU3072699.1"
                     /translation="MDFEKIEQAYTYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELNQVKDNNQALKRLALRKEEWLKTYQFLLMKAGQTEPLQANHQFTPDAIALLLVFIV
                     EELFKEEEITILEMGSGMGILGATFLTSLDKKVDYLGMEVDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGL
                     KYLKSDGYAIFLAPSNLLTSPQSDLLKEWLKEEASLVAMISLPENLFANAKQSKTIFI
                     LQKKSEIAVEPFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            20656..21846
                     /locus_tag="VVE42_000444"
     CDS             20656..21846
                     /locus_tag="VVE42_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEU3072700.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGRSEQQILDIENHTQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKHIGQYLAVLNGTDAIVFTAGVGE
                     NAENFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            21995..22366
                     /gene="rnpA"
                     /locus_tag="VVE42_000445"
     CDS             21995..22366
                     /gene="rnpA"
                     /locus_tag="VVE42_000445"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEU3072701.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNHFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYREGNGSEKETKVD"
     gene            22341..23165
                     /locus_tag="VVE42_000446"
     CDS             22341..23165
                     /locus_tag="VVE42_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEU3072702.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNGVTSDITAESADFWSKLVYF
                     FAKIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYP
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            23184..24170
                     /locus_tag="VVE42_000447"
     CDS             23184..24170
                     /locus_tag="VVE42_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEU3072703.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVDAIKKI
                     EEEGQGISDEVKAEILKHERHASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QTENQDLKEMGLKVEQSYDIAQVATDVTAYVQAIVDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNRYSKTFYVTINVNDYVEHRAEVLQTYAQKL
                     AIRVLEEGRSHQTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
     gene            24221..24844
                     /locus_tag="VVE42_000448"
     CDS             24221..24844
                     /locus_tag="VVE42_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HEU3072704.1"
                     /translation="MLNKIRDYLDFAGLQYRNPDKAGAEREKMLAFRHKGQEARKAFT
                     ELAKAFQASHPEWQLQQTSQWMNRAQRLRPHFWVYLQRDGQVTEPMMALRLYGTSTDF
                     GISLEVSFIERKKDEQTLGKQAKVLDIPTVKGIYYLTYSNGQSQRWEANEEKRRTLRE
                     KVRSQEVRKVLVKVDVPMTENSSEEEIVEGLLKSYSKILPYYLATRK"
     gene            24975..26432
                     /locus_tag="VVE42_000449"
     CDS             24975..26432
                     /locus_tag="VVE42_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU3072705.1"
                     /translation="MEVVSSVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIAAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLSIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFTFGLIGQLITIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLASYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFLLVIPQLQFAKAKDKEKYYNGEVQEEA"
     gene            26455..26736
                     /locus_tag="VVE42_000450"
     CDS             26455..26736
                     /locus_tag="VVE42_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU3072706.1"
                     /translation="MVKVLAACGNGMGSSMVIKMKVENALRKLNQTDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQALQ"
     gene            26813..27299
                     /locus_tag="VVE42_000451"
                     /pseudo
     CDS             26813..27299
                     /locus_tag="VVE42_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002267837.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
     gene            27316..27981
                     /locus_tag="VVE42_000452"
     CDS             27316..27981
                     /locus_tag="VVE42_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEU3072707.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVGQEVDIIEAGTVCLLQV
                     GSELVEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAADPAGAARAFKDEIKR
                     IWG"
     gene            27985..28848
                     /locus_tag="VVE42_000453"
     CDS             27985..28848
                     /locus_tag="VVE42_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEU3072708.1"
                     /translation="MVRPIGIYEKATPTHFTWLERLNFAKELGFDFVEMSIDERDERL
                     ARLDWSKEERLEVVKAIYETGVRIPSICFSGHRRYPLGSKDPVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKACDWAEEAQVVLAIEIMDDPFIS
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHLKDTYAVTES
                     SKGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAVQEAQAF
                     LYPLIKKAGLM"
     gene            28850..29554
                     /locus_tag="VVE42_000454"
     CDS             28850..29554
                     /locus_tag="VVE42_000454"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEU3072709.1"
                     /translation="MNQVINAMRKRVCDANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYKAWSEIGSVVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVAYEKDTGLVIVEEFEHRGL
                     NPVEVPGIVVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVGPAPQYILE
                     KHYQRKHGPNAYYGQK"
BASE COUNT         8903 a         5237 c         6817 g         8700 t
ORIGIN      
        1 tatgtcttgt tctatcgatt tattaaaaca tcggtatttg aaaaatatta aagaaaatcc
       61 tgaattgttt gtcggaattg agttggagta tcctgttgca agtttagaag gggatgctac
      121 agatgttgaa gttataaagg atctatttca ttatttagtt tctactttgg atctcaccgt
      181 agcaaaggta gatgattttg gcaatctgat ccagttagta gatccgataa gtcaggatgc
      241 tattttattt gaagtttcct atacaacgat tgagtttgca tttggtaagg ctgaaacgat
      301 tcaagaggtc gaaaatcgtt tcaataatta tatgaatgta attcagagaa agttagctga
      361 atcaaatcat gctattgttg gctgtggtat ccatcccaac tgggataaaa atgagaattg
      421 tccagtggct tatccacgct atcagatgtt gatggattat ttgaatttga gtagaaatat
      481 tattaaatca gatttacatc atttccctga atatggtact tttatctgtg ggagccaggt
      541 tcagctggat atttcaaaaa ccaactactt gcgggtgatt aatgctttta ctcaaattga
      601 agcggctaag gcttatttat ttgcaaactc tgagttttcg ggtgcggatt gggatacgaa
      661 aatttcaagg gatattttct gggaagaatc tatgcatggt atctatccag agaatgttgg
      721 ggtcaatgct agactcctta atgatgaagc tgattttttt gactatctaa atcattctgc
      781 gatttttact gcggaacgtg atgggcagac ctattatttt tatcctattc aggctgggga
      841 ctatttggct acgcctgaaa tccaagcatt tgctctgaat ggggatgagg ttattattta
      901 cccccaagag aaggattttg aaactcatcg tagttaccag taccaagact taacgactcg
      961 aggaacagtt gagtttcgta gtgtgtgtac acagccactt gataggactt ttgcttctgc
     1021 agcttttcac ttgggattat tggttaattt agacaagtta gaagcttact tagaaacagc
     1081 acctttcttt aaagtatttg gttatgatta caagttttta aggagacaat tttctaagaa
     1141 aaatcttaca gatgaggaag aaactatgat tattgaattt tccaaagact tactcctact
     1201 agctgaggag ggactagtgg tgagaaataa ggaagaaatg acctatttac agcctttgag
     1261 agaagaattg agcctataat ttctcttata aagggagaat tttctgaaaa atcatgatat
     1321 aatggacgag actatagata aaggatagag agtaatgaca ttagtttatc aatcaacgcg
     1381 tgatgccaac aatacagtaa ctgccagcca agcaattttg caaggtttgg cgacggacgg
     1441 cggtttgttt acaccggtta cttatccaaa ggtagatttg aactttaaca aattgaaaga
     1501 tgcttcttac caggaagttg ctaagctagt tttgtcagca tttttagatg actttacagc
     1561 tgaggagttg gactactgta tcaacaatgc ctacgatagc aaatttgata ctccagctat
     1621 tgcaccatta gtgaaattag atgggcaata caatttggaa cttttccatg gttcaacgat
     1681 tgcctttaag gatatggcct tgtctatttt gccatacttt atgacgactg ctgctaagaa
     1741 acatggtttg gagaacaaga ttgttatctt gacagcgaca tctggtgaca cggggaaagc
     1801 tgctatggcg gggtttgcgg atgtgcctgg tactgagatt atcgtctttt atccaaagga
     1861 tggtgtcagc aagattcaag agttacaaat gaccactcaa actggcgaca atactcatgt
     1921 tattgctatt gatggtaact ttgacgatgc gcaaacaaat gtgaagcaca tgtttaacga
     1981 cgtggctctt cgtgaaaaat tgactaccaa caagttgcaa ttttcatcag ctaactctat
     2041 gaacattggt cgtctggtgc cacaaattgt ttattatgtt tatgcttacg ctcaattggt
     2101 caagactggt gagatcgtgg ctggtgataa gattaacttc acagtaccaa caggaaactt
     2161 tggaaatatc ttggctgcct tttatgccaa acaaatcggt ctcccagttg gtaaattgat
     2221 ctgtgcttca aatgacaaca atgttttgac agacttcttc aagacacgtg tctatgacaa
     2281 gaaacgtgag tttaaggtaa caaccagccc atctatggat atcttggtat cttcaaactt
     2341 ggagcgtttg attttccatc ttttggggaa tgatgcggtt aagacggctg aactcatgaa
     2401 tgctttgaat agccaaggtc aatatgagtt gactaatttt gatgcagaga ttttggacct
     2461 ctttgcagct gaatatgcga ctgaggaaga aactgcggca gaaatcaagc gtgtttatga
     2521 gtcagattct tatatcgagg atcctcatac agcggttgcc tcagcagtgt atagaaaata
     2581 ccaggcgact actggagatg cgactaagac agtgattgct tcaacagcta gtccctacaa
     2641 gttcccagta gttgcagtag aagcgctaac agggaaagtt ggcttgactg attttgaagc
     2701 tttggctcaa ttacatgaaa tctcaggcgt tgcagtgcca ccagcagttg atgggcttga
     2761 gacagctcct gttcgtcaca agacaatagt ggcagctgct gacatgcaag tagcggttga
     2821 ggcttattta ggactttaag atagagggag taaactcggt tgggaaacca actgagtttc
     2881 ttttcatcag gaggagagat tgtttaagaa aaataaagac attcttaata ttgcattgcc
     2941 agctatgggt gaaaactttt tgcagatgct aatgggaatg gtggacagtt atttggttgc
     3001 tcatttagga ttgatagcta tttcaggggt ttcagtagct ggtaatatta tcaccattta
     3061 tcaggcgatt ttcatcgctc tgggagctgc tatttccagt gttatttcaa aaagcatagg
     3121 gcagaaagac cagtcgaagt tggcctatca tgtgactgag gcgttgaaga ttaccttact
     3181 attaagtttc cttttaggat ttttgtccat cttcgctggg aaagagatga taggactttt
     3241 ggggacggag agggatgtag ctgagagtgg tggactgtat ctatctttgg taggcggatc
     3301 gattgttctc ttaggtttaa tgactagtct gggagccttg attcgtgcaa cgcataatcc
     3361 acgtctgcct ctctatgtta gttttttatc caatgccttg aatattcttt tttcaagtct
     3421 agctattttt gttctggata tggggatagc tggtgttgct tgggggacaa ttctgtctcg
     3481 tttggttggt cttgtgattt tgtggtcaca attaaaactg ccttatggga agccaacttt
     3541 tggtttagat aaggaactgt tgaccttggc tttaccagca gctggagagc gacttatgat
     3601 gagggctgga gatgtagtga tcattgtctt ggtcgtttct tttgggacgg aggcagttgc
     3661 tgggaatgca atcggagaag tcttgaccca gtttaactat atgcctgcct ttggcgtcgc
     3721 tacggcaacg gtcatgctgt tggcccgagc agttggagag gatgattgga aaagagttgc
     3781 tagtttgagt aaacaaacct tttggctttc tctgttcctc atgttgcccc tgtcctttag
     3841 tatatatgtc ttgggtgtac cattaactca tctctatacg actgattctc tagcggtgga
     3901 ggctagtgtt ctagtgacac tgttttcact acttgggacc cctatgacga caggaacagt
     3961 catctatacg gcagtctggc agggattagg aaatgcacgc ctcccttttt atgcgacaag
     4021 tataggcatg tggtgtatcc gcattgggac aggatatctg atggggattg tgcttggttg
     4081 gggcttgcct ggtatttggg cagggtctct cttggataat ggttttcgct ggttatttct
     4141 acgctatcgt taccagcgct atatgagctt gaaaggatag gaaatgcaaa aaacagcttt
     4201 tatttgggat ttagacggga ctttattgga ctcttacgaa gcgattttat cagggattga
     4261 ggagactttt gctcagtttt ctattcctta tgataaggag aaggtgagag agtttatctt
     4321 caagtattcg gtgcaagatt tgcttgtgcg ggtggcagaa gatagaaatc tggatgttga
     4381 ggtgctaaat caggtgcgtg cccagagtct ggctgagaag aatgctcagg tagttttgat
     4441 gccaggtgcg cgtgaggtgc tagcttgggc agacgaatca ggaattcagc agtttatata
     4501 tactcataag ggaaacaacg cttttaccat tctcaaggac ttgggggtgg aatcctattt
     4561 tacagagatt ttaaccagtc agagtggctt tgtgcggaag ccaagtccag aagcggctac
     4621 ctatctgcta gataagtatc agttgaattc tgataatact tattatatag gggatcggac
     4681 tctggatgtg gaatttgccc agaatagtgg gattcaaagc atcaactttt tagagtctac
     4741 ttatgaaggg aatcacagga ttcaagcgtt agcagatatt tcccgtattt ttgagactaa
     4801 gtgataaaaa gattgtgtca gttttgtgac agagacctaa caaactattt caagtaacct
     4861 agtttgttac aaggaataga cagttctgtt aaataggccc gagagggctt tttttctaca
     4921 ttttttgtgt tatgatagac aggtactcat ttgaaaggaa tttgaaagaa tgaagaaaag
     4981 aatgttatta gcgtcaacag tagccttgtc atttgcccca gtattggcaa ctcaagcaga
     5041 agaagttctt tggactgcac gtagtgttga gcaaatccaa aacgatttga ctaaaatgga
     5101 caacaaaaca agttataccg tacagtatgg tgatactttg agcaccattg cagaagcctt
     5161 gggtgtagat gtcacagtgc ttgcgaatct gaacaaaatc actaatatgg acttgatttt
     5221 cccagaaact gttttgacaa cgactgtcaa tgaagcagaa gaagtaacag aagttgaaat
     5281 ccaaacacct caagcagact ctagtgaaga agtgacaact gcgacagcag atttgaccac
     5341 taatcaagtg accgttgatg atcaaactgt tcaggttgca gacctttctc aaccaattgc
     5401 agaagctcca aaagaagtag catcaagttc agaagttaca aagacagtga ttgcttctga
     5461 agaagtggca ccatctacgg gcacttctgt cccagaggag caaacgaccg aaacaactcg
     5521 cccagttgaa gaagcaactc ctcaggaaac gactccagct gagaagcagg aaacacaagc
     5581 aagccctcaa gctgcatcag cagtggaagt aactacaaca agttcagaag caaaagaagt
     5641 agcatcatca aatggagcta cagcagcagt ttctacttat caaccagaag agacgaaaat
     5701 aatttcaaca acttacgagg ctccagctgc gcccgattat gctggacttg cagtagcaaa
     5761 atctgaaaat gcaggtcttc aaccacaaac agctgccttt aaagaagaaa ttgctaactt
     5821 gtttggcatt acatccttta gtggttatcg tccaggagac agtggagatc acggaaaagg
     5881 tttggctatc gattttatgg taccagaacg ttcagaatta ggggataaga ttgcggaata
     5941 tgctattcaa aatatggcca gccgtggcat tagttacatc atctggaaac aacgtttcta
     6001 tgctccattc gatagcaaat atgggccagc taacacttgg aacccaatgc cagaccgtgg
     6061 tagtgtgaca gaaaatcact atgatcacgt tcacgtttca atgaatggat aaacccgact
     6121 tgataacatc attttgacga atgagatcta gctttcgtga tggaaagcga ttctcgttcg
     6181 ttttttcttt gtcatactct tcgaaaatct cttcaaacca cgtcagtttt atctgaaacc
     6241 tcaaagctgt gctttgagca acctgcgact agctttctag tttactcttt gattttcatt
     6301 gagtatcaat ttgaatggaa aatggaaagt tattatcttg taataatcca agcaacattc
     6361 ttgcaatcta ttttacttta tatcacaatt gattggtcaa ctattgataa gtcaatggat
     6421 aggaggaaga aatgatagag attcaagatt tactgtatca actccgcttg tctgagcaag
     6481 cgagtacgca attgtttgaa aaaggcttgg gattagtttg acacggtatc agattttact
     6541 gtttttgctg gagcattctc tttgtaacca aatggcggtt caggagcgtt tgaaaattga
     6601 tcaggctgct ttgacacggc atttcaagat tttggaaacg gaaggtttgg tggagcgtca
     6661 tcgtaatcct gaaaatcagc gggaagtgtt ggtagaggct gcgaagtatg ccaaggagca
     6721 gttagtggtg aatccccctc tgcaacatat caaggttaag gaagagatgg aaagtatctt
     6781 aacagagttt gagagaacag aactcagccg tttattaaat aaattggttt tgggtattga
     6841 aaatatagaa atttaaggag aaatagatgt caattatttt aacaacgatc gttgctttgg
     6901 agcattttta cattttttat ttggaaagta ttgccacgca atcagatgcg actagtcgtg
     6961 tatttaatat ggaaaaggaa gaattggctc atccgtcagt aagttcattg ttcaaaaatc
     7021 aaggaattta taaggatctg ctaggagtct ttctcttgta tggcatttat ttctcacaga
     7081 atttagaaat tgtgactatt tttgtcttat ttgtgattgg tgctgcgact tacggctctt
     7141 taacagcgga taaaaaaatt attttgaaac aaggtggatc agctattttg gccttgatta
     7201 gtattttact ctttaaatac acttgaaggt cgattctaat ctcgctaatc ctttttaatc
     7261 cagaataagg gaaatatgtt atacttgttt ttaagaaaaa agtctcattg aattggtttt
     7321 gaggagttag aaatgaaagt attagtgaca ggttttgagc cctttggagg ggaaaagggc
     7381 aatccagctt tggaggccat taaaggttta ccagctgaaa tccatggtgc tgaggtccgt
     7441 tggctagagg tgccgacagt tcttcacaaa tctgctcaag tattggaaga agagatgaat
     7501 cgttatcaac ctgactttgt cctttgtatt gggtaagctg gtggaagaac tagtttgaca
     7561 cctgaacgag tggccattaa tcaagacgat gcacgcattt ctgataacga agataatcaa
     7621 ccgattgacc gtcccattcg cccagatggt gcttcggcct actttagtag tttgccgatt
     7681 aaagcgatgg ttcaagctat aaaaaagaag gattgccggc ctctgtttcc aatacggcag
     7741 ggacttttgt ctgcagccat ttgatgtatc aggctctcta tttggtagaa aagaaatttc
     7801 catatgttaa ggcaggtttt atgcatattc cttatatgat ggaacaggtg gtgaacagac
     7861 cgactattcc agctatgagt ttagtggata ttcggcgagg gatagaagca gcaatcggcg
     7921 ctatgataga acatggagat caggaactca agttggtagg cggagaaact cattgataga
     7981 aaaaagcttg aggggaaaaa ccttcaagct tttggacgtt ttcgagccaa tactgctcgg
     8041 taaaacataa ttttagtgca ttggatataa ggtaggagtg aaaaactagc aatgccaaag
     8101 gtaatccaat tgaggaagta ccaaggaaga agctgtaaat ctaggacaaa gtgctggaac
     8161 ttgtagccct tcataaagga acggctagtt tttaggattc gtcttggtgg gacctgtcct
     8221 aggtctagac tataacagag aagaaattcc acctgtgaat aggcataata ctgtggaata
     8281 tagaggatat tccctacaat gatcaagatg agacttgcaa gaaagtagag tccaaagacc
     8341 atgaggaaac gctcggtttc aactgatgag agatctagat ttggaaactc aggatgtagg
     8401 gtgacgaatt ttttggctaa aaagctacta taaaagagga ggtaaatccc aagtaaatta
     8461 gggatactcc ataaaaagag atagaaacgt ttgagaagta gggtcaaaaa ggtttgagaa
     8521 aagcgctcct catcaaagag agctaggctg ttttttacag atggctccgt tttagaatct
     8581 ttcatgagtg tcagtgttgc atagacggaa ctggtcaaaa gaatagtccc gataaaggag
     8641 actagtagag gaaagaggta ggcttgaagt atttggccaa gtatgctgaa aaatggctgt
     8701 tctaaaacag tcccgtggat ccgagataag ggattaagaa aactagataa gatgaccagc
     8761 atactgggaa ggatatagag gagaaagaga cggggggtgt cagcctgaaa atgttttgac
     8821 tcctgacgaa ttgtttttaa atcaattttt ggatagttca ttctcttatt ataccatagt
     8881 tcgtgacagt tcctactttt tttgataaaa tcatacagtg tgtccttggg cacactgtat
     8941 gaactgggac tgtctttccc agcttcggag gtaaaaaatg tcagattcac caatcaaata
     9001 tcgtttgatt aagaaagaaa aacacacagg agctcgtcta ggagaaatca tcactcccca
     9061 cggtaccttt ccgacaccta tgtttatgcc agttgggaca caagccactg tcaaaactca
     9121 gtcacctgaa gaattgaagg agatgggttc gggaattatc ctatcaaaca cctatcatct
     9181 ctggcttcgc cctggagatg aactcattgc acgcgctggt ggtctccaca agttcatgaa
     9241 ttgggaccag cctatcttga cagatagtgg tggttttcag gtttattctt tagcagatag
     9301 ccgtaatatc acagaagaag gagtaacctt taaaaatcat ctaaatggtt ctaagatgtt
     9361 cctatcccca gaaaaagcca tctctattca gaataatctg ggttcagaca tcatgatgtc
     9421 ctttgatgaa tgtcctcagt tttatcaacc ttatgactac gttaagaaat cgatcgagcg
     9481 taccagccgt tgggctgagc gtggtttgaa ggctcaccgt cgtccacatg accaaggttt
     9541 gtttggaatt gtgcaaggtg caggatttga agaccttcgc cgccaatcag ctcatgatct
     9601 tgtcagcatg gatttctcag gctactctat cggtggtttg gcagtgggag aaacccatga
     9661 agagatgaat gcggtcttgg actttacaac tcaactgcta cctgaaaata aacctcgtta
     9721 tctgatgggt gtgggagcgc cagatagctt gatcgatggg gtcattcgtg gggtggatat
     9781 gtttgactgt gtcttaccga ctcgaattgc tcgtaacggg acttgtatga ccagtcaagg
     9841 acgtttggtt gtgaaaaatg cccagtttgc tgaggacttt acgccactgg atcctgagtg
     9901 tgactgctac acatgtaata actatacacg cgcttacctt cgtcacctgc tcaaggctga
     9961 tgaaaccttt ggtatccgct tgactagcta ccacaatctt tacttcttgc ttaacctgat
    10021 gaagcaagtg cgacaagcca tcatggatga caatctcttg gaattccgtg agtattttgt
    10081 ggaaaaatat ggctataata agtcaggacg taatttctaa aatggaattg atataaaaaa
    10141 atcctaagtt ttctcttagg atttttcttc tttttttgat agaataaagt gtacaatgaa
    10201 aggaagaata aactcgtatg cgcattaaat ggttttcctt gattaggatt ataggtttac
    10261 ttttggtact cttgtatcac ttctttcaga cgatctttcc tggaggattt ttcggggtag
    10321 atgtcttttt cacattttca ggtttcctga ttacagctct actcattgaa gaattttcta
    10381 agaacaatga gattgatttg ataggatttt ttagaagacg cttttatcgg attgtgccac
    10441 ctgtggtttt gatggtcttg gtgaccatgc cttttacttt cttggttcgc caagactatg
    10501 ttgctggaat tggtggccag attgcgggcg tcttaggctt tatgaccaac ttctatgaac
    10561 tcctaacagg tgggagttat gaatctcagt tcattcctca tttgtttgtt cataattgga
    10621 gtttggcagt tgaggttcac tactatattc tttggggatt ggcagtttgg ttcttatcca
    10681 aacaagctaa atcaaatggt cagttgaagg ggatggtctt tctcttatct gctgctgcct
    10741 tcttgatcag tttcttctcc atgtttattg gtagttttct agtgacctct tattcctctg
    10801 tttatttctc cagtttaact catgtctatc cattcttttt gggaagtatg ttagcaacta
    10861 ttgtaggcgt tcgtcagacg acttccctcg tcaagcagtt ggataaaatc tgggatttac
    10921 gaaagacttt ggtagttttt ggaggaggct ttggtttcct agttcttttg actttctttg
    10981 tcaaattcac ttatcttttt gcctatctta tcggcttctt gcttgccagt cttgcagctc
    11041 ttgccatgat tctggcggcg cgtgtcttac atgaaaagac acatcatata caggagccga
    11101 agattatcag ctttttagcg gatactagct atgcggttta tcttttccat tggcctttct
    11161 atatcatttt ctcacagttg acatcaaatc ttcttgctgt attactgact ctgatttgtt
    11221 cttatggctt tgccagtctg tcattctatg tattggaacc ttggattgta ggcaagaaca
    11281 cacctatttt acaaactctt cgtcccctgc cttatattca cgcaattctt gcagcaggta
    11341 caggaatctt gaccttcatt gtctgcatgg tgaccttgtt ggcaccacaa gtgggagcgt
    11401 ttgagacaga cttgactgtc aatggcttga agcaagctgc aacaaatatt ggccagacca
    11461 aggtgatggc agaacgggca gatgcaaaca gtttgggaat tgctgatggc actatgttaa
    11521 ttggtgactc agtggcttta agggcaaata cagcactaca gacagctctt cctggagcac
    11581 agattaacgc gcaggtcagc gtaacaacca agaccgcaaa tgaaatcatg ctaaataata
    11641 gccagaataa atttttacct aagacggtgg tcattgcgac tggggtaaat aatcctgaga
    11701 attacaagga tgactgggac agtatcgtga aaaatcttcc taagggacac catatgattt
    11761 tggtgactcc ttatgaggga gataagacaa aagagaccta tgccatcgtt gagaaggctg
    11821 ctgcctatat gagagaattg gcagagaaga caccttacat tacgatagca gattggaatc
    11881 aagttgcgaa agagcatcca gaaatttggg caggaacaga ccaggttcat ttcgggagtg
    11941 agagtagcac tatcgaagca ggagcaaaat tgtatgcaga tacgattgcc acagctttgc
    12001 agacagctca agataagccg gttaaatcaa aataacttgt attaaaaaag agaagagttg
    12061 gagaaatcca gctcttttaa aatatctcca aaaaataggt ctataccatt tacaaataaa
    12121 aaagaaaggt ttataatgta attgacataa taaattgtag aatcaatctt ttaaggaggt
    12181 taacattatg cctaactata ttaaagcgga tcagtttttc tacccacacg gagttcgtcg
    12241 aggtggttac ttggaacttg tggacggcaa gtttgggaaa catgtagagc agattcctga
    12301 aggggctgag gtgattgact atacaggtta tagcattgcc ccaggtcttg tggatactca
    12361 tattcatgga tatgcaggtg tagatgtgat ggacaacaac attgaaggta cattgcatac
    12421 tatgagtgaa ggacttctta gtaccggtgt taccagtttc ttacccacaa ctttaacagc
    12481 cacttatgag caattgcttg cagtcactga aaatcttgga aaccattata aagaagcaac
    12541 aggtgctaag attcgtggga tttattatga aggtccatat ttcacagaaa cttttaaggg
    12601 ggcacaaaat ccaacttata tgagagaccc gggtgttgag gagtttcatt cttggcaaaa
    12661 agcggcaaat ggcttgctta ataaaattgc ccttgcacca gaacgtgatg gggtggaaga
    12721 ctttgttcgt acagttacgg gcgaaggtgt gacggttgct cttggacatt caaacgcgac
    12781 ttttgatgaa gccaaaaaag cagtcgatgc tggagcgagt gtttgggtgc atgcctacaa
    12841 tggaatgcgt gggttgactc accgtgaatt gggtatggtt ggagccatgt accaattgcc
    12901 atatacctat gcagagttga tctgtgatgg tcaccacgta gatccaaagg cttgcgaaat
    12961 tcttatcaaa caaaaaggaa cagaaaacat cgctcttatc acagactgta tgacagctgg
    13021 gggattggaa gacggagatt atatgttggg agaattccca gttgtcgttg caaatggaac
    13081 tgcacgcctc aaatcgacag gtaacttggc aggttctatc ctcaaactca aagatggttt
    13141 gaagaatgtg gtcgaatggg gaattgcgaa tccgcatgaa gcagtcatga tggccagctt
    13201 caacccagct aaatccgttc acatcgatga cgtctgtggc caaatccgtg aaggctacga
    13261 cgctgacttc atcgtattag ataaagattt ggaattggta gcaacctacc tagatggtgt
    13321 gaaacgttat caagcataag agagaaggca gaagttagag actagcttct gcttttttat
    13381 acagggtact ttactggtaa ataaaaagtt aaaaaaatca caaaaaaagc ttgaagaaac
    13441 aaagaaaaag gcttacaatt gaactataaa tattacaaat aaaaaaacgg aggagtgctt
    13501 tatgaaagcc tatacttatg ttaaaccagg acttgcttct tttgttgatg tagacaaacc
    13561 agttattcgc aagccaacag atgctattgt gcgtattgta aaaaccacta tttgtggaac
    13621 agacctccat attatcaaag gggatgttcc tacttgccaa agtggtacca ttcttggcca
    13681 cgaagggatt gggattgttg aagaagttgg ggaaggagtt tccaacttca aaaaaggtga
    13741 caaggtcttg atttcttgcg tctgtgcctg tggtaaatgc tactactgta aaaaaggaat
    13801 ttatgcccac tgtgaagacg aagggggctg gattttcggt cacttgattg atggtatgca
    13861 ggctgaatat ctacgtgtcc ctcatgcaga taatactctt taccatactc cagaagactt
    13921 gtcagatgaa gctttggtta tgctgtcaga cattctgcct actggatatg aaattggtgt
    13981 cttaaaaggg aaagtagaac ctggttgcag cgtagccatt attggttcag gtccagttgg
    14041 attggctgct cttttaacag cccaattcta ttcaccagct aaattgatta tggtggactt
    14101 ggatgataac cgtttggata ctgcagtatc ctttggtgct actcataagg taaactcttc
    14161 agaccctgaa aaagcgataa aagaaattta tgatttgaca gatggtcgcg gtgtagatgt
    14221 cgctattgaa gctgtaggga ttcctgaaac atttgatttc tgtcaaaaga ttatcggtgt
    14281 agacggaacg gttgccaact gtggtgtgca tggtaaacca gttgaattcg atttagataa
    14341 actttggatt cgcaacatca atgtaacaac tggtttggta tctacaaata cgactccaca
    14401 attgttgaaa gcacttgaaa gtcataagat tgaaccagaa aaattggtaa ctcactattt
    14461 caaactcagt gaaattgaaa aagcctacga agtcttcagt aaggcagcag accaccatgc
    14521 cattaaggtc attatcgaaa acgatatctc agaagcctaa gtagtaaaaa tatttttgta
    14581 cataagcaaa tagaaattca gtcatccatc agatggctgg attttttatc aaaaaattaa
    14641 gaaatgagca tatttctttc cttgtctggc ggaattggtt ataatatacg gtacaaagga
    14701 atgaatgaat atgtatcgtg ttatagaaat gtacggagat tttgaaccgt ggtggttctt
    14761 agaaggttgg gaagaagata ttgtagcaag tagaaaattt gaccagtatt atgatgctct
    14821 caaatactac aaaacttgct ggtttagatt ggaacaagaa tcccctcttt ataaaagtag
    14881 aagcgacttg atgaccattt tttgggaccc ggaagaccaa cgctggtgtg atgaatgtga
    14941 tgagtattta caacaatacc attctttggc tcttttgcag gatgagcagg ttatcccaga
    15001 cgaaaaacta cgctcaggct atgaaaaaca aaccagtcag gaaaggaatc gttcttgccg
    15061 tatgaaatta aaatagagaa aagtaacttt tttggagttg ctttttttat ttttctaacg
    15121 ctttgcgaat agtataggtg aggaggtaag tatggttcaa gaaattgcac aagaaatcat
    15181 tcgttcggct cggaaaaaag gggcgcaaga catttatttt gtccccaagt tagatgccta
    15241 tgagcttcat atgagggtag gagacgagcg ctgtaaaatt ggtagctatg attttgaaaa
    15301 gtttgcagcc gttatcagtc actttaagtt tgtggcgggt atgaatgtgg gagaaaaaag
    15361 acgtagtcaa ctgggttcct gtgattatgc ctatgaccag aagatagcgt ctctacgttt
    15421 atctactgta ggcgattatc gggggcatga gagtttggtt atccgtttgt tgcacgatga
    15481 ggagcaggac ttgcattttt ggtttcagga tattgaagaa ttaggcaagc agtacaggca
    15541 acggggactc tatctttttg ctggtccggt tgggagtggt aagacgacct tgatgcatga
    15601 attgtccaag tcactcttta aaggacagca agttatgtcc atcgaagatc ctgtcgaaat
    15661 caagcaggac gacatgcttc agttgcagtt gaacgaagca atcggcctaa cctatgaaaa
    15721 tctaatcaaa ctttccttgc gtcatcgacc agatctcttg attatcggag aaattcgtga
    15781 cagcgagacg gcgcgtgcag tggtcagagc tagtttgaca ggtgcgacag tcttttcaac
    15841 cattcacgcc aagagtatcc gaggtgttta tgagcgtctg ctggagttgg gtgtgagtga
    15901 agaagaattg gcagttgttc tgcaaggagt ctgctaccag agattaatcg ggggaggagg
    15961 aatcgttgac tttgcaaaca gagattatca agaacaccaa gcagccaagt ggaatgagca
    16021 aattgaccag cttcttaaag atggacatat cacaagtctt caggctgaga cggaaaaaat
    16081 tagctacagc taagcaaaaa aatatcatca ccctatttaa caatctcttt tctagcggtt
    16141 ttcatctggt ggagactatc tcctttttag ataggagttc cttgttggac aagcagtgtg
    16201 tgacccagat gcgtacaggc ttgtctcagg ggaaatcatt ctcagaaatg atggaaagtt
    16261 tgggatgttc aagtaccatt gtcactcagt tatctctagc tgaagttcat ggcaatcttc
    16321 acctgagttt gggaaagata gaagaatatc tagacaatct ggccaaggtc aagaaaaagt
    16381 taattgaagt agcgacctat cctttgattt tgctgggttt tcttctctta attatgctgg
    16441 ggctacggaa ttacctgctc ccacaactgg atagtagcaa tattgccacc cgaattatcg
    16501 gtaatctgcc acaaattttt ctaggcatgg tagggcttgt ttccgtgctt gcccttttag
    16561 cactaacttt ttataaaaga agttctaaga tgagtgtctt ttctatctta gcacgccttc
    16621 cctttattgg aatctttgtg cagacctact tgacagccta ttatgcacgt gaatggggga
    16681 atatgatttc acagggaatg gagctgacgc agatttttca aatgatgcag gaacaaggtt
    16741 cccagctctt taaagaaatc ggtcaagatc tggctcaaac cctgaaaaat ggccgtgaat
    16801 tttctcagac gataggaacc tatcctttct ttaggaagga attgagtctc atcatagagt
    16861 atggggaagt taagtccaag ctgggtagtg agttggaaat ctatgctgaa aaaacttggg
    16921 aagccttttt tacccgagtc aaccgcacca tgaatttggt gcagccactg gtttttatct
    16981 ttgtggcact gattatcgtt ttactttatg cggcaatgct catgcccatg tatcaaaata
    17041 tggaggtaaa tttttaaaat gaaaaaaatg atgacattct tgaaaaaagc taaggttaaa
    17101 gcttttacat tggtggagat gttagtagtc ttgctgatta tcagcgtgct tttcttgctc
    17161 tttgtaccta atctgaccaa gcaaaaagaa gcagtcaatg acaaaggaaa agcagctgtt
    17221 gttaaggtgg tggaaagcca ggcagaactt tatagcttgg ataaaaatga agatgctact
    17281 ctgagcaagt tacaaggaga tgggcgcatc acggaagaac aggttaaagc ttataatgaa
    17341 tactatacta aaaatggagg ggcaaatcgt aaagtcaatg attaaggcct ttaccatgct
    17401 ggaaagtctc ttggttttgg gacttgtgag tatccttgcc ttgggcttat ccagctctgt
    17461 ccagtccact tttgcagcag tagaggagca gattttcttt atggagtttg aagaactcta
    17521 tcgggaaacc caaaaacgca gtgtagccag tcagcaaaag actagtctaa atttagatgg
    17581 gcagacgctt agcaatggca gtcaaaagtt gacagttcct aaaggaattc aggcaccatc
    17641 aggccaaagt attacatttg accgagctgg gggcaattcg tccctggcta aggttgaatt
    17701 tcagaccagt aaaggagcga ttcgctatca attatatcta ggaaatggaa aaattaaacg
    17761 cattaaggaa acaaaaaatt agggcagtga ttttactgga agcagtagtc gctctagcta
    17821 tctttgccag cattgcgacc ctccttttgg gacaaattca aaaaaatagg caagaggaag
    17881 caaaaatctt gcaaaaggaa gaagtcttga gggtagctaa gatggccctg cagacggggc
    17941 aaaatcaggt aagcatcaac ggagttgaga ttcaggtatt ttctagtgaa aaaggattgg
    18001 aggtctacca tggttcagaa cagttgttgg caatcaaaga gccataaggt caaggctttt
    18061 accttgttag aatccctgct tgccctcatt gtcatcagtg ggggattact cctttttcaa
    18121 actatgagtc agctcctcat ttcagaagtt cgctaccagc aacaaagcga gcaaaaggag
    18181 tggctcttgt ttgtggacca acttgaggca gaattagacc gttcgcagtt cgaaaaagta
    18241 gaaggcaatc gcctatacat gaagcaagat ggcaaggaca tcgccatcgg taagtcaaag
    18301 tcagacgatt ttcgtaaaac cgatgctagt ggacgggggt atcagcctat ggtttatggc
    18361 ctcaaatcag ctcagattac agaggacaat caactggttc attttcgttt ccagtttcaa
    18421 aaaggcttag aaagggagtt catctatcgt gtggaaaaag aagaaagtta aggcaggtgt
    18481 tctcctctac gcagtcacca tagcagccat ctttagtctt ttgttgcaat tttatttgaa
    18541 ccgacaaatc gcccactatc aagactatgc tttaaataaa gaaaaattgg ttgcttttgc
    18601 catggctaaa cgaaccaagg ataaagccga gcaagaaagt ggggaacagg tttttaatct
    18661 aggtcaggta agctatcaaa acaagaaaac aagcttggtg acgactgttc gtacgtctaa
    18721 gagccaatat gagtttctgt ttccttcagt caaaatcaaa gaagagaaaa gagataaaaa
    18781 ggaagaggta gcgaccgatt caagcgaaaa agcggagaaa aaaatcagaa gagaagcctg
    18841 aaaagaaaga gaattcctag tcaattcaac tataatgcgt tgaatccaga atagtccact
    18901 gtagtttcta gaaaattgct ggaaatggat gttaagctcc aattcatttg tttatatctt
    18961 atttcagttt actatacttt gtgctaaatt aaagatatga aacatgattt taaccacaaa
    19021 gcagaaactt tcgattcccc taaaaatatc ttcctcgcaa acttggtatg tcaagcagcc
    19081 gagaaacaga ttgatcttct atcagacaaa gaaattttag atttcggtgg tgacacgggt
    19141 ctattagcct tgcccctagc ccctagccaa gcaggctaag tcagtcactc ttgtagacat
    19201 ttctgagaaa atgttggagc aagctcgttt gaaagtggag cagcaagcaa tcaagaatat
    19261 ccagtttttg gagcaagatt taccgaaaaa tcccttggag aaagagtttg atttccttgc
    19321 tgttagtcgg gttcttcatc atatgcctga tttggatgcg gctctctcac tgtttcatca
    19381 acatttgaag gaagatggga aactcatcat tgctgatttt accaagacag aagctaatca
    19441 tcatggattt gatttagctg aactggaaaa caagctaatt gagcatggtt tttcatctgt
    19501 gcatagtcag attctctata gcgctgaaga cctgtttcaa ggaaatcact cagaattctt
    19561 tttaacagta gcccaaaaat cactcgccta gtcagggagt gatttttcta taaggatgga
    19621 aaaaagaagg gaaatttgat aagataggaa tatggatttt gaaaaaattg aacaagctta
    19681 tacctattta ctagagaatg tccaagtcat ccaaagtgat ttggcgacca acttttatga
    19741 cgccttggtg gagcaaaaca gcatctatct ggatggtgaa actgagctaa accaggtcaa
    19801 agacaacaat caggccctta agcgtttagc actacgcaaa gaagaatggc tcaagaccta
    19861 ccagtttctc ttgatgaagg ctgggcaaac agaacccttg caggccaatc accagtttac
    19921 accggatgct attgctttgc ttttggtgtt tattgtggaa gagttgttta aagaggagga
    19981 aattactatc ctcgaaatgg gttctgggat gggaattcta ggcgctactt tcttgacctc
    20041 gcttgataaa aaggtggatt acttgggaat ggaagtggat gatttgctga ttgatctggc
    20101 agctagcatg gcagatgtaa ttggtttgca ggctggcttt gtccaaggag atgccgttcg
    20161 tccacaaatg ctcaaagaaa gcgatgtggt catcagtgac ttgcctgtcg gctattatcc
    20221 tgatgacgcc gttgcgtcgc gccatcaagt tgcttctagc caagaacata cttacgccca
    20281 tcacttgctc atggaacaag ggcttaagta cctcaagtca gacggatacg ctatttttct
    20341 agctccgagt aatttgttga ccagtcctca aagtgatttg ttaaaagaat ggctgaaaga
    20401 agaggcgagt ctggttgcta tgattagtct gcctgaaaat ctctttgcta atgctaaaca
    20461 atctaagact atttttatcc tacagaagaa aagtgaaata gcagtggagc cttttgttta
    20521 tccacttgct agcttgcaag atgcaagtgt tttaatgaaa tttaaagaaa attttcaaaa
    20581 atggactcaa ggtactgaaa tataaaatag attttgttat aatagttgaa aacgcttaaa
    20641 aaggggtatc atgttatgac aaaaacaatt gcaatcaatg caggaagttc aagtttgaaa
    20701 tggcaattat acttaatgcc agaggaaaaa gtgttagcca aaggtttgat tgaacgtatc
    20761 ggtttgaaag attcaatttc aactgtaaaa tttgacggcc gttctgaaca acaaattttg
    20821 gatatcgaaa atcatacaca agctgttaaa attttattgg atgacttgat tcgtttcgat
    20881 attatcaagg cttatgacga gattacaggt gttggccacc gtgttgtagc aggtggagaa
    20941 tatttcaaag aatcaacagt tgttgaggga gatgttttag aaaaagttga agagttgagt
    21001 ttgttggctc ctctacacaa cccggccaat gcagcaggtg ttcgtgcttt caaggaattg
    21061 ttgccagaca ttaccagtgt agttgttttt gatacttcct tccacacaag tatgccagag
    21121 aaagcttatc gctaccctct accaacaaaa tattacacag aaaacaaagt tcgtaaatac
    21181 ggtgctcatg gtacaagtca ccagtttgta gcaggagaag ctgcaaaact cttgggacgt
    21241 ccattagaag acttgaagtt aattacctgt catattggta acggaggctc aattacagct
    21301 gtgaaagccg gcaaatctgt agacacttct atggggttca ctcctcttgg tggtattatg
    21361 atgggaacgc gtacagggga tattgatcca gctatcattc cttatttaat gcaatataca
    21421 gaggatttta acacaccaga agatatcagt cgtgttctta accgtgaatc aggtcttttg
    21481 ggagtttctg ctaattctag cgatatgcgc gatatagaag cagctgtagc agaagggaat
    21541 cacgaggcta gcttggctta tgaaatgtat gttgaccgta tccaaaaaca tatcggtcag
    21601 taccttgcag tgctaaatgg aacagatgcc attgttttca cagcaggtgt cggtgaaaat
    21661 gcagagaatt tccgtcgtga tgtaatctca gggatttcgt ggtttggttg tgatgttgat
    21721 gatgaaaaga atgtctttgg cgttacagga gacatctcaa cagaggcagc taaaatccgt
    21781 gtcttggtta ttccaacaga tgaagaatta gtcattgccc gtgacgttga acgcttgaaa
    21841 aaataagtga aactaaaaaa atattcaata caaggagttg ggaaagttat ttttccagct
    21901 tctttttctg atgaaattgt ccaaaacctt gctatgattg gcttttttga aaaatatggt
    21961 ataatagtag taatttaata gatggagttg agttttgaag aaaaactttc gtgtaaaaag
    22021 agagaaagat tttaaggcga ttttcaagga ggggacaagt tttgctaatc gcaaatttgt
    22081 ggtctaccaa ttagaaaacc agaaaaacca ttttcgagta ggtctatcag ttagcaaaaa
    22141 actggggaat gccgtcacta gaaatcaaat taagcgacgg attcggcata ttatccagaa
    22201 tgcaaaaggg agtctggtag aagatgtcga ctttgttgtc attgctcgaa aaggagtcga
    22261 aaccttggga tacgcagaga tggagaaaaa tctactccat gtattaaaat tatcaaagat
    22321 ttaccgggaa ggaaatggga gtgaaaaaga aactaaagtt gactagtttg ctaggactgt
    22381 ctctgttaat catgacagcc tgtgcgacta atggggtaac tagcgatatt acagccgaat
    22441 cggctgattt ttggagtaaa ttggtttact tctttgcgaa aatcattcgc tttttatcgt
    22501 ttgatattag tatcggagtg gggattattc tctttacggt cttgattcgt acagtcctct
    22561 tgccagtctt tcaggtgcaa atggtggctt ctaggaaaat gcaggaagct cagccacgca
    22621 ttaaggcgct tcgagaacaa tatccaggtc gagatatgga aagcagaacc aaactagagc
    22681 aggaaatgcg taaagtattt aaagaaatgg gtgtcagaca gtcagattct ctttggccga
    22741 ttttgattca gatgccggtt attttggcct tgttccaagc cctatcaaga gttgactttt
    22801 taaagacagg tcatttctta tggattaacc ttggtagtgt ggatacaacc cttgttcttc
    22861 cgattttagc agcagtattc acctttttaa gtacttggtt gtccaacaaa gctttgtctg
    22921 agcgaaatgg cgctacgact gcgatgatgt atgggattcc agtcttgatt tttatctttg
    22981 cagtttatgc gccaggtgga gtcgccctat actggacagt gtctaatgct tatcaagtct
    23041 tgcaaaccta tttcttgaat aatccattca agattatcgc agagcgcgag gccgtagtac
    23101 aggcacaaaa agatttggaa aatagaaaaa gaaaagccaa gaaaaaggct cagaaaacga
    23161 aataaataag gaggaatctg gtaatggtag tatttacagg ttcaactgtt gaagaagcaa
    23221 tccagaaagg attgaaagaa ttagatattc caagaatgaa ggctcatatc aaagtcattt
    23281 ctagggagaa aaaaggcttt cttggtctat ttggtaaaaa accagcccaa gtggatattg
    23341 aagcgattag tgaaacgact gttgtcaaag caaatcaaca ggtagtaaaa ggcgttccga
    23401 aaaaaatcaa tgatttgaac gagcctgtga agacggttag tgaagaaacc gttgaccttg
    23461 gtcatgtggt tgatgctatt aaaaaaatag aggaagaagg tcaaggtatt tctgatgaag
    23521 tcaaggctga aatcttaaaa catgaaagac atgccagcac tatcttagaa gaaactggtc
    23581 acattgagat tttaaatgaa cttcaaatcg aggaagcgat gagggaagaa gcaggcgctg
    23641 atgaccttga aactgagcaa gatcaaactg aaaatcaaga cttgaaagag atgggcttga
    23701 aggtcgagca aagttatgat attgcccagg tggctacgga tgtgactgcc tatgttcaag
    23761 cgattgtgga tgacatggat gttgaagcta cactttcaaa tgattataac cgtcgtagca
    23821 tcaatctaca aattgacacc aacgaaccag gtcgtattat cggctaccat ggtaaagtct
    23881 tgaaggcctt gcaactgttg gctcaaaatt atctttacaa ccgctattcc aaaaccttct
    23941 acgttacaat caatgtcaat gattatgtcg aacaccgtgc agaagtcttg cagacctatg
    24001 cgcaaaaatt ggcgattcgt gttttagaag aaggacgtag tcatcaaaca gatccaatgt
    24061 cgaatagtga acgcaagatt atccaccgta ttatttcacg tatggatggc gtgaccagtt
    24121 actctgaagg tgatgagcca aatcgctatg tcgttgtaga tacagaataa gtaaaatcag
    24181 ggttgacctg attttttgct agctagagga ggttaaactg atgttgaata agataagaga
    24241 ctatttagac tttgctggtt tgcagtaccg taatcctgat aaagcgggag cagagcgaga
    24301 gaagatgctg gcattccgcc acaaaggaca agaggcccga aaggctttta cagaactggc
    24361 caaagccttt caagcaagcc atccagaatg gcaactccaa cagactagtc agtggatgaa
    24421 tcgggcccag cgtttgagac cacatttttg ggtttatcta cagagagacg gacaagtgac
    24481 agaacctatg atggccttac gtttgtatgg gacatctact gactttggaa tttctttgga
    24541 agtcagtttc atcgaacgta agaaggatga gcaaacactg ggcaagcagg ccaaagtttt
    24601 agacattcca accgttaaag ggatttatta tctaacctac tctaatggtc aaagtcaacg
    24661 gtgggaggcg aatgaagaaa agcgtcgtac tttacgcgag aaggtgagaa gtcaagaagt
    24721 tcgaaaagtt ttagtgaagg tagatgttcc tatgacagaa aattcgtctg aagaagaaat
    24781 cgtagaaggc ttattgaagt cttattctaa aattcttccc tattatctag ctacgagaaa
    24841 ataagataat ttgtaaaaca tcataaatca tacagtccaa gagtgaacag tccgctgtgt
    24901 aattcttggt ctttttgttt gcgctttcgc attatataat aaacttacaa aaacaattca
    24961 aaaggagaac aattatggaa gtcgtttcaa gtgttctaaa ttggttttct agcaatattt
    25021 tgcagaatcc cgcatttttc gtaggtttat tggtgttgat aggatatgca cttttgaaaa
    25081 aacctgccca tgacgttttt tcagggtttg ttaaagcaac agtagggtat atgttgctta
    25141 acgtgggtgc tggtggtttg gttacaacct ttcgtccaat cttagcagct cttaactaca
    25201 aattccaaat tggtgcagcg gttatcgacc cttactttgg acttgctgca gcaaacaaca
    25261 aaattgcagc agagtttcca gattttgttg gaactgcaac tacagctcta ttgattggtt
    25321 ttggaataaa tatcttgctc gtagctcttc gaaagattac gaaggtaaga accctcttta
    25381 ttactggtca catcatggta caacaagctg caacagtatc tcttatggtt ctattcttag
    25441 taccacaatt gcgcaatgct tacggtacag cagcgattgg tatcatctgt ggactttact
    25501 gggcagttag ttcaaatatg actgttgagg caactcaacg cttgactggt ggtggcggat
    25561 ttgcgattgg tcaccaacag caatttgcaa tctggtttgt agataaagta gcaggacgct
    25621 ttggtaagaa agaagaaagt ttagacaatc ttaaattacc taagttcctc tcaatcttcc
    25681 acgatacagt tgttgcatct gctaccttga tgctcgtatt cttcggagcc attcttttaa
    25741 tcttgggtcc agacattatg tctaataaag aagtcatcac ttcaggaact ctattcaatc
    25801 ctgctaaaca agatttcttt atgtacatta tccaaacagc ctttaccttc tcagtttact
    25861 tgttcgtttt gatgcaaggt gtccgaatgt tcgtatctga gttaacaaac gctttccaag
    25921 gtatttcaaa caaattgttg ccaggttcat tcccagcggt tgacgttgca gcttcttatg
    25981 gatttggttc tccaaatgct gtcttgtcag gatttacctt tggtttgatt ggtcaattga
    26041 ttacaattgt cttgctcatc gtctttaaaa atccgattct tattattaca ggatttgtac
    26101 cagtgttctt tgacaatgca gccattgcgg tctacgctga taaacgcggc ggatggaaag
    26161 cggctgttat cctttccttt atatcaggtg tccttcaagt tgctctagga gctctttgtg
    26221 tggcccttct cgatttggca tcttatggtg gctaccatgg aaatatcgac tttgaattcc
    26281 catggcttgg atttggatat atcttcaaat accttggtat tgttggttat gtacttgtgt
    26341 gtctcttctt gcttgttatt cctcaacttc aatttgccaa agcaaaagat aaagagaaat
    26401 attacaacgg tgaagttcaa gaagaagctt agtatctaga aaaggagaaa taaaatggtt
    26461 aaagtattag cagcgtgcgg aaatggaatg ggttcatcaa tggttatcaa gatgaaggtt
    26521 gaaaatgctc tccgtaagct taatcaaaca gattttacag tcaattcatg cagtgtcggt
    26581 gaagctaaag gtttagcagt aggatatgac atcgtaatcg cttctcttca tttgattcaa
    26641 gaattggaag ggcgaactaa tgggaagtta attgggcttg ataacttgat ggatgataaa
    26701 gaaatcaccg aaaaactcag tcaagcacta cagtaaaagg ttggaggggg ctggacagaa
    26761 actgagagtt atcgtttctg tccttctccc tctttaaata aaggaggcag atatgaattt
    26821 aaaacaagct ttaaattgac aatgactcga tccgactagg tttagaggct aacaattgga
    26881 aagaagcagt caaggtagca gtagatccct taattgaaag tggggcaatt ttgccagagt
    26941 attacgatgc tatcattgaa tcgactgaag agtatgggcc ttactatatc ttgatgccag
    27001 gtatggctat gccccacgct agacctgaag ctggtgtgca aagtgatgcc ttttcattga
    27061 ttaccttaca aaatcctgtt gtattttcag atgggaaaga ggtatctgtt ttgttggcac
    27121 tagcagcaac aagctcaaaa attcacacaa gtgtagccat tccacaaatt attgccctgt
    27181 ttgaattaga agattctatt gcacgtttac aggcttgcca gactaaagaa gatgtcttgg
    27241 ctatgattga agaatctaag gatagccctt atctcgaagg attggatttg gaaagttaga
    27301 aagaggaata aagaaatgac aaaaagaata cctaatttac aagttgcatt agaccattca
    27361 gacttgcaag gagcgattaa agcagctgtt tctgttggtc aggaagtaga tattatcgaa
    27421 gctggaactg tttgcttgct tcaagttgga agtgaactgg ttgaagtctt gcgtagtctt
    27481 ttcccagata agattattgt ggcagacaca aaatgtgctg atgctggtgg aacagttgct
    27541 aaaaataatg cggttcgtgg agcagactgg atgacttgta tctgttgtgc aaccatccct
    27601 actatggaag cagctctaaa ggctatcaag actgaacgag gagaacgagg cgaaatccag
    27661 atcgagcttt atggcgattg gacttttgaa caagctcagc tttggctaga tgcaggtatc
    27721 tcacaagcta tttatcacca atctcgtgat gctcttcttg ctggtgaaac ttggggtgaa
    27781 aaagacctta ataaggttaa aaaactcatt gacatgggct tccgtgtatc tgtaacaggt
    27841 ggtctagatg tagatactct caaactcttt gaaggtgttg atgtctttac ctttatcgca
    27901 ggtcgtggaa ttacagaggc tgcggatcca gcaggagcag cgcgtgcctt caaggatgaa
    27961 atcaaacgaa tttgggggta aatcatggta cgtccaattg gaatttatga aaaggcaacc
    28021 ccaacacact ttacttggct agaacgttta aattttgcca aggagttagg ctttgatttt
    28081 gtcgagatgt ctattgacga acgtgacgag cgtttagcaa gacttgactg gagtaaggaa
    28141 gaacgcttgg aagttgtcaa agcaatctat gaaactggtg ttcgtattcc ttctatctgt
    28201 ttttcaggcc atcgtcgcta cccattgggt tcaaaagatc cagttctaga ggaaaaatct
    28261 ctagaactca tgaaaaaatg tatcgaatta gctcaagact tgggagttcg tacgattcaa
    28321 ttagctggtt acgatgttta ctatgaggaa aagtcacccc agacacgcca acgttttatc
    28381 aaaaatttga gaaaagcctg tgactgggct gaagaagctc aggtggtact tgctattgaa
    28441 attatggatg atcctttcat cagtagcatc gaaaaatatt tggctataga aaaagagatt
    28501 gactctccct tcctctttgt atatccagat attggtaatg tgtctgcatg gcataatgat
    28561 atctatagtg agttttatct tggtcatcat gccatcgcag ctctccatct caaggatact
    28621 tatgcagtga cagaaagttc aaagggccag ttccgagatg tacctttcgg gcaaggttgt
    28681 gtcaaatggg aagaagcttt cgatatttta aaggaaacca attataatgg acctttccta
    28741 atcgaaatgt ggtctgaaaa ttgtgaaaca gtagaagaaa cacgcgcagc cgttcaagag
    28801 gcgcaagctt ttctctatcc actcattaag aaagcaggtt tgatgtaaga tgaatcaagt
    28861 aatcaatgct atgcgtaaac gagtctgtga tgccaatcaa tcattgccaa aacatggact
    28921 tgtcaaattt acctggggga atgtatctga agttaatcgc gaactcggtg tcattgttat
    28981 caaaccatca ggcgtggatt atgacgaatt gacacctgaa aacatggtag tgactgatct
    29041 agatggtaag atcctagaag gggatttaag accatcttcc gacctcccaa ctcatgtgca
    29101 attatataag gcttggtcag aaattggtag tgtggttcac acccattcga cagaagctgt
    29161 tggttgggct caggcaggtc gtgatattcc tttctacgga acaacccatg cagattattt
    29221 ctacggttca atcccttgcg cccgtagttt gaccaaggac gaagtagaag tggcctatga
    29281 aaaagatact ggcctggtta tcgtagaaga gtttgaacat cgcggactta acccggttga
    29341 agtaccagga attgttgtac gcaatcacgg tccattcacc tggggcaaaa atccagagaa
    29401 tgctgtttat cactctgtcg tactagagga agtatcaaag atgaatcgct ttacagaaca
    29461 aatcaatcca agagttggac ctgctcccca gtacatacta gaaaaacact accaacgtaa
    29521 acatggacca aatgcttatt atggtcaaaa gtaagaacga tgaaggagga gaaaaagata
    29581 aatttagctc ctctttttac atttgatttt tattgagagt aaagttggaa ttgaagtaat
    29641 tttaaaagat tttttag
//
LOCUS       DAVGJL010000012        28047 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.012, whole genome shotgun sequence.
ACCESSION   DAVGJL010000012 DAVGJL010000000
VERSION     DAVGJL010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28047
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.012"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(310..2568)
                     /gene="glgP"
                     /locus_tag="VVE42_000455"
     CDS             complement(310..2568)
                     /gene="glgP"
                     /locus_tag="VVE42_000455"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HEU3072710.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYQVPFADFTLTSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIELDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAAVKEKLESIKAHNKRKLARHLKEHQGVEINPNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGEENIYIFGEDSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERLYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(2594..4111)
                     /gene="malQ"
                     /locus_tag="VVE42_000456"
     CDS             complement(2594..4111)
                     /gene="malQ"
                     /locus_tag="VVE42_000456"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEU3072711.1"
                     /translation="MKKRQSGVLMHISSLPGAYGIGSFGQSAYDFVDFLVRTKQRYWQ
                     ILPLGATSYGDSPYQSFSAFAGNTHFIDLDILVEQGLLEASDLEGVDFGSDASEVDYA
                     KIYYARRPLLEKAVKRFFEVGDVKDFEKFAQDNQSWLELFAEYMAIKEYFDNLAWTEW
                     PDADARARKASALESYREQLADKLVYHRVTQYFFFQQWLKLKAYANDNHIEIVGDMPI
                     YVAEDSSDMWANPHLFKTDVNGKATCIAGCPPDEFSVTGQLWGNPIYDWEAMDKDGYK
                     WWIERLRESFKIYDIVRIDHFRGFESYWEIPAGSDTAAPGEWVKGPGYKLFAAVKEEL
                     GELNIIAEDLGFMTDEVIELRERTGFPGMKILQFAFNPEDESIDSPHLAPANSVMYTG
                     THDNNTVLGWYRNEIDDATREYMARYTNRKEYETVVHAMLRTVFSSVSFMAIATMQDL
                     LELDEAARMNFPSTLGGNWSWRMTEDQLTPAVEEGLLDLTTIYRRINENLVDLKK"
     gene            4661..5932
                     /locus_tag="VVE42_000457"
     CDS             4661..5932
                     /locus_tag="VVE42_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU3072712.1"
                     /translation="MSSKFMKSTAVLGTATLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKVTLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIAGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            6043..7335
                     /locus_tag="VVE42_000458"
     CDS             6043..7335
                     /locus_tag="VVE42_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU3072713.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGDKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            7337..8179
                     /locus_tag="VVE42_000459"
     CDS             7337..8179
                     /locus_tag="VVE42_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU3072714.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            8432..9232
                     /locus_tag="VVE42_000460"
     CDS             8432..9232
                     /locus_tag="VVE42_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEU3072715.1"
                     /translation="MLPYPFSYFSSIWGFRKPLSKRFELNWFQLLFTSIFLINLSMVP
                     IAIQNSSQETYPLETFIDKVYEPLTDKVVQDLSEHATIVDGTLTYTGTASQAPSVVIG
                     PSQIKELPKDLQLHFDTNELVISKESKELTRISYRAIQTENFKSKDSLTQAISKDWYQ
                     QNRVYISLFLVLGASFLFGLNFFIVSLGASLLLYITKKSRLFSFRTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDPNYHK"
     gene            9242..10228
                     /locus_tag="VVE42_000461"
     CDS             9242..10228
                     /locus_tag="VVE42_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU3072716.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
     gene            10363..10443
                     /locus_tag="VVE42_000462"
                     /pseudo
     CDS             10363..10443
                     /locus_tag="VVE42_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(10564..11505)
                     /locus_tag="VVE42_000463"
     CDS             complement(10564..11505)
                     /locus_tag="VVE42_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEU3072717.1"
                     /translation="MKQAKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLSFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINSLFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(11483..13246)
                     /gene="aspS"
                     /locus_tag="VVE42_000464"
     CDS             complement(11483..13246)
                     /gene="aspS"
                     /locus_tag="VVE42_000464"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEU3072718.1"
                     /translation="MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEQEIQDITEGLIARVMKETKGIEVTLPFPHMKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVKGVDFKVFSEAPAVKAIVVKGAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALALEDKDLVLFVADT
                     LEVANATLGALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EETAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSTEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETSKTN"
     gene            complement(13482..13562)
                     /locus_tag="VVE42_000465"
                     /pseudo
     CDS             complement(13482..13562)
                     /locus_tag="VVE42_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(13598..13828)
                     /locus_tag="VVE42_000466"
     CDS             complement(13598..13828)
                     /locus_tag="VVE42_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3072719.1"
                     /translation="MKRVILLAVIQAVVLFFIIGALAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
     gene            complement(13846..14487)
                     /locus_tag="VVE42_000467"
     CDS             complement(13846..14487)
                     /locus_tag="VVE42_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU3072720.1"
                     /translation="MKLLKNLGWFLLALLSFFFIYGFIQGLATASLALGASPYAVTLL
                     YVVLAGVYVYGIYKWYQKAPVYIEKSGFNRFIWLPALVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRTLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYISRKGLVYSISLHALNNLVGFLMILML"
     gene            complement(14499..15233)
                     /locus_tag="VVE42_000468"
     CDS             complement(14499..15233)
                     /locus_tag="VVE42_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEU3072721.1"
                     /translation="MKKKRRLLFLMSIVLGGFLGMFVGMFKARVESHEIILDVKALMP
                     WISAICLLIGFISMFLTFNFLKKSRKFHSLYQEEMDDDLNETYYVQMYRNLEFGTIAF
                     NITGVAIPLAIFISLSEVIILHTNPQTFFLSFLLFVVFLVAQKSLFKTIAIVRQFDLE
                     FFATPKDVLNYINSYDEGERQANLEQSFRILFQLHQYVLPALYIFLIIISFLTGEIQL
                     LAFLLVGAIHVYINVMQLPMVKRYFK"
     gene            complement(15236..15433)
                     /locus_tag="VVE42_000469"
     CDS             complement(15236..15433)
                     /locus_tag="VVE42_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3072722.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNPYQSDQFVWVYFA
                     LIPVIEWIEKKSKNLASEKGE"
     gene            complement(15433..15639)
                     /locus_tag="VVE42_000470"
     CDS             complement(15433..15639)
                     /locus_tag="VVE42_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU3072723.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            complement(15649..15963)
                     /locus_tag="VVE42_000471"
     CDS             complement(15649..15963)
                     /locus_tag="VVE42_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3072724.1"
                     /translation="MWVLGFILFMIFFYSNNSKKIKKLEKKIKRLERKEKGNAEMSRL
                     LQEMIGKEPIITGVYIGPDNWEVVDVDEEWVKLRRVDNTGKEKFKLQRIEDIQTVEFD
                     GE"
     gene            complement(16259..17548)
                     /locus_tag="VVE42_000472"
     CDS             complement(16259..17548)
                     /locus_tag="VVE42_000472"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEU3072725.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIVMAAHFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVA
                     VENAPSILDFLDEESQTHFDAVRQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQGVTLPIENALDVYIA
                     VLGEGANIKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFVAKTLITLGESEVES
                     GQVTVKNNQTREEVQVSLETISQNFSEIFEKLGFYTQ"
     gene            complement(17626..18873)
                     /locus_tag="VVE42_000473"
     CDS             complement(17626..18873)
                     /locus_tag="VVE42_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEU3072726.1"
                     /translation="MKLLFRNQAYRLLTLSRFFNAFGASIFNLVFIVYASTLPQASFA
                     VAMANIVMILPTLFTVFAGIRADYTRDKVKWMVYSGLFQAVLFFLAALVVQQASLFAF
                     SSLCLINVISDVISDFAGGLRMPLIKEKVAEDDLMEAYSFSQFITYISAIGGQAFGVW
                     LLALSVNNFSLVAGINACFFLVSATILFLGKSKLSLSMSSADGENLKNEKLSIKDRFL
                     TIYRNLRLVFLKSGQKNFGFMLFAVLLINSLGGALGGIYNIFFLSHSLLNFSYTEALF
                     INQFCVLVAIIISSLTGNDYFGKQSLPRLMMWETVGFSLVGLANLFNQVVLGLLFLFF
                     TLYVSGKVQPKISAMLMKNLAPEVLARTSNFFGLLFTLSIPVGTACFSLVAVWNMQLT
                     WMLFVGFSLLAIFLTILNLKNDI"
     gene            complement(19063..19914)
                     /gene="rgg"
                     /locus_tag="VVE42_000474"
     CDS             complement(19063..19914)
                     /gene="rgg"
                     /locus_tag="VVE42_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434947.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
                     /protein_id="HEU3072727.1"
                     /translation="MEQIGKVFRQLRESRNISLRQATGGQFSPSMLSRFETGQSELSV
                     EKFLFALENISASVEEILFLARGFQYDTDSELRKEITDVLEPKNIAPLEDLYRREYQK
                     HAHSHNKQKHILNAIMIKSYMKSMDERVELTAEEGKVLHDYLFSTEIWGIYKLNLFSV
                     SSPFLSVSLFTRYVREMVRKSDFLMEMSGNRNLFHTILLNGFLASIECEEFTNAYYFK
                     RVIEEHFYKENETYFRIVYLWAEGLLDSKQGRVKEGQKKMEDAVRIFEMLGCNKSAEY
                     YRNTTEC"
     gene            20038..20379
                     /locus_tag="VVE42_000475"
     CDS             20038..20379
                     /locus_tag="VVE42_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEU3072728.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIKDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
     gene            20459..20605
                     /locus_tag="VVE42_000476"
     CDS             20459..20605
                     /locus_tag="VVE42_000476"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3072729.1"
                     /translation="MDRTDEVSSKHCFEVVDRTDEVSPKHCFEVVDRTDEVSNHTYGK
                     ATLT"
     gene            complement(20631..22334)
                     /gene="ilvD"
                     /locus_tag="VVE42_000477"
     CDS             complement(20631..22334)
                     /gene="ilvD"
                     /locus_tag="VVE42_000477"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEU3072730.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETSIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIVDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAETTLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
     gene            complement(22567..23499)
                     /locus_tag="VVE42_000478"
     CDS             complement(22567..23499)
                     /locus_tag="VVE42_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEU3072731.1"
                     /translation="MMRSTKELRHVYRDFLLEANQSDSDIVVLEADLSSSMATHNLEK
                     DFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFSSRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPKSIIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPVPVYQGGEDFSKGYIELRHGEDVVIVASGIMVAPSIRVADELSKLGYSI
                     GVIDLFRIKPIPEQIKTMLSGKTIFTVENHNQIGGIGSALCELFSDDGITKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVLEIYNAR"
     gene            complement(23496..24353)
                     /locus_tag="VVE42_000479"
     CDS             complement(23496..24353)
                     /locus_tag="VVE42_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU3072732.1"
                     /translation="MILSKNREDGLRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNPGDFVEKFSAFGFESIRVKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRPTVEERQMLTSVVER
                     LSQELEETE"
     gene            complement(24357..25707)
                     /locus_tag="VVE42_000480"
                     /pseudo
     CDS             complement(24357..25707)
                     /locus_tag="VVE42_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
     gene            complement(25720..26004)
                     /locus_tag="VVE42_000481"
     CDS             complement(25720..26004)
                     /locus_tag="VVE42_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU3072733.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKLCEEKGLI"
     gene            complement(26008..28038)
                     /locus_tag="VVE42_000482"
     CDS             complement(26008..28038)
                     /locus_tag="VVE42_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEU3072734.1"
                     /translation="MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NSHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLIDQVVKDYYEKLQLVPIVNQLKVYLFGLVF
                     ILCRYQRDVERVGLSETLVSPVIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEHYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKWYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKASSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYDDIKMIIKQED"
BASE COUNT         8603 a         6108 c         5056 g         8280 t
ORIGIN      
        1 aatacaagag atatgaggta accaactacg aaaaaatata aggctgaaga cagcaaaaac
       61 cagcatttaa agccttcact gtatctagga tttccctatt tttagacaag attgacccaa
      121 atccacagca tttaaaaaag cgaatttcaa tcgaaactcg cttttttaca agatgtatta
      181 gtataaattt gtatcttata ctcaatgaaa atcaaagagc aaactaggaa gctagccgca
      241 ggttgctcaa agcaccgctt tgaggttgca gataaagctg acgtggtttg aagagatttt
      301 cgaagagtat tagttcaagt gccagatgtc ttcattatac tgagcgattg tacggtcaga
      361 tgagaagaat cctgctttag aaatgttaac gatgacttta tccaaccatg cgtcacggtc
      421 ttcgtagtca gcaagcattt gctctttgac tttgatgtag tcttccaaat caagaagagt
      481 catgaaccag tctttgttga tcaattcatt gtaaagacgt tccaagcgct ctttgtttcc
      541 agctgcaaga actgcatcac taacgatgaa gtcaaccaat ggtttgatag cttcacgagc
      601 gtagaattcg cttgatttgt aagctgcttt tgcgtaaagg tcgataacag tttctgaatc
      661 ttcaccgaag atgtagatgt tttcttctcc aaccaactca gcgatttcca cgttagcacc
      721 gtccatagta ccaagtgtca aagctccgtt caacatgaat ttcatgttac cagtacctga
      781 agcttcttta gaagcaagtg agatttgttc tgagatatca catgctggga taaggaaact
      841 tgctgcagta acgttgtagt tttcaaccat aactacttgc aagtgtggag ctactgctgg
      901 atcgttagca ataacttctg acatgcaaag gattaaatgg ataatgtctt gagcgattgt
      961 gtaggctgga gctgctttac caccaaagaa gattgtgatt ggacgagcag ggatgttacc
     1021 agctttgatg tcaaggtatt tgtggatcac gtacaaagcg ttcatttgtt ggcgtttgta
     1081 ctcgtgaaga cgtttgattt ggatatcaaa gatagaattt ggattgattt ccacaccttg
     1141 gtgttctttc aagtgacgag ccaatttacg tttgttgtga gccttgatgc tttccaattt
     1201 ttctttgaca gctgctttgt cttcataaga caaaagtttt tcaagctcat ctgcttcatg
     1261 gtgccaacca tctccaagaa tctcatccaa gtagtgagac aatcttgggt tagcatgcat
     1321 aagccaacga cggaaagtga taccgtttgt tttgttgttg aacttttctg ggtaaaggtc
     1381 gtagaaggct ttcaactcag aatttttcaa gatttcagta tggagtgctg caaccccgtt
     1441 aacactgtat ccgtagtgga tatccatgtg agccatgtga acacgtccgc tctcatcgat
     1501 gatttgaaca gctggatctt tgtactctgc cttcacacga cggtccaatt cttcgatgat
     1561 tggtaccaag tgaggaacca cttcttgcaa gaattcaaga ggccattttt caagcgcctc
     1621 agcaaggatt gtgtggttag tgtaggcagt catgctacga acaattgaaa ttgcttcgtc
     1681 aagctcgata ccacgtgcag tcaaaagacg aatcaattca ggaatcacca ttgatgggtg
     1741 agtatcgttg atttggacaa ctgcgtagtc agcaaggtca tgcaagttgc ttcctttttc
     1801 gattgcttcg tcgatgatca attgcgcacc gtttgaaacc atgaagtatt gttgaaagat
     1861 acggagcaat tcaccttgac ggtcactatc atctgggtaa aggaagagag tcaagttgcg
     1921 agcgatatct gtcttgtcaa agttgatacc atctttaata atagaagaat caactgaatc
     1981 caagtcaaac aaacgcaagc ggtttttagt cgctgtttca taaccagtaa catcaatatc
     2041 gtaaagagtt gatgtcaaag taaagtctgc aaatggtact tggtagctac ggcttgagcg
     2101 aaccaaccag ttttgctctg tcaaccatgc atttggaatt gtttcttgtt ggttgttttt
     2161 aagaacttgt tggaaaagac caaagtggta gttaagacca acaccgtcac cattcaaacc
     2221 aagagtagca attgagtcga taaagcaggc agccaaacgt cccaaaccac cattaccaag
     2281 agatggttcc aattcaactt cttcaacttc gatcaagtct ttacctgcag ctgcaagttc
     2341 ttttttaaca tcgtcgtaaa gaccaaggtt aatcaagttg tttgacaaga gtttaccaat
     2401 caagaactca gctgatatgt agtaaacttt tttcttacca gtgttgactg gtttttggct
     2461 gcttgcaagc ttgctgtagt taagaagagc aaggtaaagc tcttcattgc tacattctgc
     2521 aatggtttta ttgtaacgat tttgtacaaa ttcttgtagt gataacatgt ttagttgtct
     2581 cctgattatt gtcttatttc tttaaatcta ccaaattttc attaattcgg cgataaattg
     2641 ttgtcaagtc aagcaaacct tcctcgacag ctggtgtcaa ttgatcttca gtcatacgcc
     2701 aagaccagtt tccaccaagg gtagatggga agttcatacg agctgcctca tccaattcta
     2761 gtaaatcttg catagttgca attgccataa agctaactga tgaaaatact gtacgaagca
     2821 tagcgtgtac cactgtttcg tattctttac ggttcgtgta acgagccatg tactcacgag
     2881 tcgcatcatc aatctcatta cggtaccaac caagaaccgt attgttatcg tgtgttcctg
     2941 tgtacataac tgagttagca ggtgccaagt gtgggctatc aatgctttcg tcttctgggt
     3001 tgaaggcaaa ttgaagaatc ttcattcctg ggaagccagt acgttcacgt aattcgatca
     3061 cttcatctgt catgaagcca aggtcttctg cgatgatgtt tagctcacca agttcttcct
     3121 taacggctgc aaaaagcttg tagcctggac ctttcaccca ctcaccaggt gctgctgtat
     3181 cggaaccagc agggatttcc caataagatt cgaagccacg gaagtggtcg atacgaacga
     3241 tatcgtagat tttgaagctt tcacgcaagc gttcaatcca ccatttgtag ccgtctttgt
     3301 ccattgcttc ccagtcatag attgggttac cccaaagctg accagttaca gaaaactcat
     3361 ctggtgggca tcctgcgata caagtagcct taccattgac atctgttttg aagagatgtg
     3421 gatttgccca catatcactt gaatcttccg ctacgtagat tggcatgtcc ccaacgattt
     3481 caatgtggtt gtcgttagcg taagctttca atttcaacca ttgttggaag aagaagtatt
     3541 gagtcacacg gtggtaaacc aacttgtctg ccaattgctc acgatagctt tcaagtgctg
     3601 aagctttacg agcacgagca tctgcatctg gccattcagt ccaagcaaga ttgtcaaaat
     3661 actctttgat agccatatac tcagcaaaga gctcaagcca tgattggtta tcttgagcaa
     3721 atttctcaaa atctttaaca tctccgactt caaagaaacg tttcaccgct ttttctaaaa
     3781 gaggacgacg tgcatagtag attttagcat agtcaacttc agacgcatcg ctaccaaagt
     3841 caactccttc aaggtcactt gcttccaaca aaccttgctc caccaagata tctaaatcga
     3901 taaaatgagt gtttcctgcg aaggctgaga aagattggta aggagaatcc ccgtaactag
     3961 ttgctcctaa tggaaggatt tgccagtaac gttgttttgt acggaccaag aaatcaacga
     4021 agtcgtaagc actttgacca aatgatccga ttccgtaagc tcctggaaga gaagagatgt
     4081 gcatcaacac accactttga cgttttttca taatagcacc tcgtgtgtta aaataatgga
     4141 acgttgcgta ttttgcagac gcaaacgttt gcgttactta taagtatact ccttttcaag
     4201 cataattgca agcgttttta aaaaactttt ttgattattt ttaaggagac taactcattt
     4261 tagcaaagat aacttaaaac tatctaatcc taaggtttga ctctcacgag acagtctaca
     4321 aagtacaaaa cctccttagg gagcttgaat ttatatgtaa caaagcacaa acgtccatag
     4381 aaaatagata gggattagaa ccagggaggt agcccctcct ggtttccctc tttagacaga
     4441 ttccatatat ttttattaaa aatactgtat tcttatctat ttcatacttt tcttctatta
     4501 aaatgcaaaa ttatgctatc aaatcaagct taaaaatttt ttaaaatttt tacaaaaaat
     4561 acttgcaacc gttttctatt tgtgctatac taagctcata aaggaaaacg tttgcgtttc
     4621 cttatcaata aaacttgcta ttctttggga ggaatacact atgtcatcta aatttatgaa
     4681 gagcactgcg gtgcttggaa ctgctacact tgctagcttg cttttggtag cttgcggaag
     4741 caaaactgct gataagcctg ctgattctgg ttcatctgaa gtcaaagaac tcactgtata
     4801 tgtagacgag ggatataaga gctatattga agaggttgct aaagcttatg aaaaagaagc
     4861 tggagtaaaa gtcactctta aaactggtga tgctctagga ggtcttgata aactttctct
     4921 tgacaaccaa tctggtaatg tccctgatgt tatgatggct ccatacgacc gtgtaggtag
     4981 ccttggttct gacggacaac tttcagaagt gaaattgagc gatggtgcta aaacagacga
     5041 cacaactaaa tctcttgtaa cagctgctaa tggtaaagtt tacggtgctc ctgccgttat
     5101 cgagtcactt gttatgtact acaacaaaga cttggtgaaa gatgctccaa aaacatttgc
     5161 tgacttggaa aaccttgcta aagatagcaa atacgcattc gctggtgaag atggtaaaac
     5221 tactgccttc ctagctgact ggacaaactt ctactataca tatggacttc ttgccggtaa
     5281 cggtgcttac gtctttggcc aaaacggtaa agacgctaaa gacatcggtc ttgcaaacga
     5341 cggttctatc gcaggtatca actacgctaa atcttggtac gaaaaatggc ctaaaggtat
     5401 gcaagataca gaaggtgctg gaaacttaat ccaaactcaa ttccaagaag gtaaaacagc
     5461 tgctatcatc gacggacctt ggaaagctca agcctttaaa gatgctaaag taaactacgg
     5521 agttgcaact atcccaactc ttccaaatgg aaaagaatat gctgcattcg gtggtggtaa
     5581 agcttgggtc attcctcaag ccgttaagaa ccttgaagct tctcaaaaat ttgtagactt
     5641 ccttgttgca actgaacaac aaaaagtatt atatgataag actaacgaaa tcccagctaa
     5701 tactgaggct cgttcatacg ctgaaggtaa aaacgatgag ttgacaacag ctgttatcaa
     5761 acagttcaag aacactcaac cactgccaaa catctctcaa atgtctgcag tttgggatcc
     5821 agcgaaaaat atgctctttg atgctgtaag tggtcaaaaa gatgctaaaa cagctgctaa
     5881 cgatgctgta acattgatca aagaaacaat caaacaaaaa tttggtgaat aaaaaatttg
     5941 ttcaaggggg gtggaaatca aatccccctt tgaatttatc aatagagaca caaataattt
     6001 agctttctta taaaaaagta gtatcctatg aaaggagtta atatggaaaa gcaacaacct
     6061 agtaaagcag ccctgctgtc tatcattcct gggttaggac agatttacaa taaacaaaaa
     6121 gccaaaggtt ttatcttcct tggtgtaacc atcgtatttg tcctttactt cctagcactt
     6181 gcaacccctg aattgagcaa cctcatcact cttggtgaca aaccaggtcg tgataattcc
     6241 ctctttatgc tgattcgtgg tgccttccat ctaatctttg taatcgttta tgtactcttt
     6301 tatttctcaa atatcaaaga tgcacatacg attgcaaaac gcattaacaa tggaattcca
     6361 gttccacgca cactcaaaga catgatcaaa gggatttatg aaaatggctt cccttacctc
     6421 ttgatcattc catcttatgt tgccatgacc ttcgcgatta tcttcccagt tatcgtaacc
     6481 ttgatgatcg cctttaccaa ctacgacttc caacacttgc caccaaacaa gttgttggac
     6541 tgggttggtt tgaccaactt tacaaacatt tggagcttga gtaccttccg ttctgccttt
     6601 ggttctgttc tttcttggac tatcatttgg gctttggcag cttctacttt acaaatcgta
     6661 attggtatct tcacagctat cattgccaac caaccattta tcaaaggaaa acgtatcttt
     6721 ggtgttattt tccttcttcc ttgggctgtc ccagccttca tcactatctt gacattctca
     6781 aacatgttta acgatagtgt cggtgctatc aacactcaag tattgccaat cttggctaaa
     6841 ttccttcctt tccttgatgg agctcttatt ccttggaaaa cagacccaac ttggactaag
     6901 attgccttga ttatgatgca aggttggctc ggattcccat acatctacgt tttgactttg
     6961 ggtatcttgc aatctattcc taacgacctt tacgaagcag cttatattga cggtgccaac
     7021 gcttggcaaa aattccgcaa catcactttc ccaatgattt tggctgttgc ggcacctact
     7081 ttgattagcc aatacacctt caactttaac aacttctcta tcatgtacct cttcaatggt
     7141 ggaggacctg gtagtgtcgg aggtggagct ggttcaaccg atatcttgat ctcatggatc
     7201 taccgtttga caacaggtac atctcctcaa tactcaatgg cggcagctgt taccttgatt
     7261 atctctatca ttgtcatctc aatctctatg atcgcattca agaaactaca cgcatttgat
     7321 atggaggacg tctaagatga ataactcaat taaactcaaa cgtagactga ctcaaagcct
     7381 tacttacctt tacctgattg gtctatcaat tgtaattatc tatccactgt tgattaccat
     7441 tatgtcagcc tttaaagcag gtaacgtctc agcctttaaa ctagatacta atatcgacct
     7501 caattttgat aactttaaag gcctcttcac tgaaaccttg tacggtactt ggtacctcaa
     7561 cactttgatt atcgccttaa ttaccatggc tgttcaaaca agtatcatcg tacttgctgg
     7621 ttatgcttac agccgttaca acttcttggc tcgtaaacaa agtttggtct tcttcttgat
     7681 catccaaatg gtgccaacta tggccgcttt gacagccttc ttcgttatgg cgcttatgtt
     7741 gaacgccctt aaccacaact ggttcctcat cttcctctac gttggtggtg gtatcccgat
     7801 gaatgcttgg ctcatgaaag gctacttcga tacagtgcca atgtctttag acgaatctgc
     7861 aaaactagac ggtgcaggac acttccgccg cttctggcaa attgttctac cacttgttcg
     7921 cccaatggtt gccgtacaag ctctctgggc cttcatggga cctttcgggg actacatcct
     7981 ctctagtttc ttgcttcgtg agaaagaata ctttactgtt gccgtaggtc tccaaacctt
     8041 cgttaacaat gcgaaaaact tgaagattgc ctacttctca gcaggtgcta tcctcatcgc
     8101 ccttccaatc tgtattctct tcttcttcct acaaaagaac tttgtttcag gacttacaag
     8161 tggtggcgac aagggataat ttatccccgc cacccttttt cattttatac tcttcgaaaa
     8221 tctcttcaaa ccacgtcagc tttatctcca acctcaaagt tgtgctttga gcaacctgtg
     8281 gctagtttgc actttgattt tcattgatta ttagcaattg tcactgtaaa taatatcctt
     8341 gtagcaagca atttttctcc tagacttgaa ataaagcgca tttctctata taataatact
     8401 catatagaaa acacctttta gaaagatacc tatgcttcca tatccatttt cctatttttc
     8461 aagtatttgg gggtttcgta agcccctgtc caaacgtttc gagctcaact ggtttcaact
     8521 tctctttacc agtatcttcc ttatcaactt gtctatggta cccattgcta tccaaaacag
     8581 ctcccaggag acctatccgc tagaaacttt tatcgataag gtctatgaac ctctgacaga
     8641 taaggttgtc caggatctct ctgaacatgc tacaattgtc gatggcacat taacttatac
     8701 tggaacagct agtcaagccc cttctgttgt gattggtcca agtcaaatca aggaattacc
     8761 taaggacttg caactgcatt tcgatacaaa tgagctagtc atcagcaagg aaagcaagga
     8821 actgacccgc atctcttacc gagccattca gactgagaat ttcaaaagca aagacagctt
     8881 gacccaagca atttctaaag actggtacca acaaaatcgt gtctatatca gcctcttcct
     8941 agttctcggt gcgagcttcc tctttggttt gaatttcttt atcgtctctc taggagctag
     9001 tcttctcctt tatatcacta agaaatcacg cctcttttca tttagaacct ttaaagagtg
     9061 ctatcacttt atcttgaact gtttaggatt gccgactctg attacactta ttttgggatt
     9121 atttggccaa aatatgacaa ccctgattac tgtacaaaat attctttttg ttctgtatct
     9181 ggtcactatc ttttataaaa cacatttccg tgatccaaat taccataaat aggagatttt
     9241 tatgcccgtt acgattaaag acgtggccaa ggctgctggt gtttcgcctt caaccgtaac
     9301 ccgtgttatt caaaataaat caaccattag cgacgaaaca aaaaaacgcg ttcgcaaagc
     9361 tatgaaggaa ctcaactacc acccaaacct caacgctcgt agcttggtaa gcagctatac
     9421 tcaggttatc ggattagttc ttcctgatga ctcagacgcc ttctaccaga atcctttctt
     9481 tccatcggtt ctacgtggca tctctcaagt cgcatctgaa aaccactatg ccattcagat
     9541 agcaacaggg aaagatgaga aggagcgtct caacgctatt tcacaaatgg tctacggcaa
     9601 gcgtgtagat gggctaatct ttctctatgc ccaagaagaa gaccctctcg taaaactcgt
     9661 cgcagaagaa cagttcccct tccttatcct aggtaaatct ctatctcctt tcatcccact
     9721 tgtcgacaac gacaatgttc aagctggttt tgatgcgact gaatatttca tcaaaaaagg
     9781 ctgcaaacgc attgccttta tcggaggaag taaaaagctc ttcgtgacca aagaccgttt
     9841 aacgggctat gaacaggcgc ttaaacatta caaacttacc actgacaaca atcgcatcta
     9901 ctttgccgac gagtttctgg aagaaaaggg ctataaattt agcaagcgat tattcaagca
     9961 cgatccacaa attgatgcta tcatcacaac cgatagcctc ctagctgaag gtgtttgtaa
    10021 ctatattgcc aaacaccagc tggatgtccc tgttctcagc tttgactcgg ttaatcccaa
    10081 gctcaacttg gcagcctatg tcgatatcaa tagtttagag cttggtcgtg tttcccttga
    10141 aactattctc cagattatta atgataataa aaacaataaa caaatttgtt accgtcaatt
    10201 gatcgcccac aaaattatcg aaaaataaga gactgggcaa aagtcgttaa aagcaaaaac
    10261 gcatactatc aggtattgaa aaaacttgat actatgcgtt ttattgtggg aagatttact
    10321 tccttttctt ctgaaattga gttgttaccc aagatctttt tatactcaat aaaaatcaaa
    10381 gtgcaaacta ggaagctagc cgcaggttgc tcaaaacact gttttgaggt tgtggataga
    10441 actgacgaag tcagctcaaa acatggtttt gaggttgtag atgaaactga cgaagtcagt
    10501 aaccatacct acggcaaggc gacgctgacg tggtttgaag agattttcga agagtattaa
    10561 tcactaatta tctatctcaa caaatcttcc tagaatatga acattttccg agacagagac
    10621 aaaggagctt ggatccactt gtgtcataat ctgtttaaat tcattaaact ctgcacgtgt
    10681 aatgacagtg attaaaactg cctttctctc gtgattatag gttccttctg catcgtggat
    10741 catggttgct ccgcggtgca attttttatg gattttttca attaccttct ctggatgatt
    10801 tgtcacaatc atggcctgca tacgcttttg cttagtaaag actgcgtctg tcacacggct
    10861 agagacaaag atggtaatca tagaataaag agcgtatttc caaccaaagg tcaaacctgc
    10921 tatcagcatg atagttccat ttaccaagaa agaaatacta ccgacattct tacccgtttt
    10981 cttgcgaata gtcaggctga cgatatccgt cccaccactg gagatattgt ttcgaagagc
    11041 aaaaccaatc cccaaaccca taacaacacc cccaaaaagg gaattgataa tgggatcctc
    11101 cgtcaaggtt gccacaggga caaactggat aaagaaggaa ctcatagata ccgtgataaa
    11161 ggtaaagacg gtgaacttat ggccaatctg ataccaagct aagaccatca aagggaagtt
    11221 aatggcgtag aagcttagcg aaatcggaat atgaaaacca aaccagtgat tactcaaggc
    11281 agagataatc tgtgccagac ctgttgcacc actcgaatac acatgccctg gttggaaaaa
    11341 gaaattaact gctactgctg ataaaaaacc atagaccaga gaggccgaaa tcttctcatc
    11401 atacttttct cgagagatac tttgtaagac acgtaaaatt tttatctgat aagcaaagcg
    11461 gcgcagataa tagcgccatc gcttaattcg ttttgcttgt ttcatcttct tctacttgta
    11521 agctgagttc ctctagttgt ttgagagcga ctgttgatgg agcttgtgtc attgggtcag
    11581 ttgccttgtt gttcttagga aaggcaatga cttcacggat attttcttct ccagcaagca
    11641 acatgacaaa acggtcaagc ccgatagcca aaccaccgtg tggtgggaaa ccatagtcca
    11701 tggcttcaag aaggaaacca aactggtcat tggcttcttc agttgagaaa ccaagagcct
    11761 tgaacatgcg ttcttgaagg tctttttggt tgatacgaag gctaccacca ccaagctcat
    11821 aaccgttcaa gacgatatcg taagcaatgg cacgaacctt agccaaatca ccttctaatt
    11881 cgtgagcagt ctcttcctgt ggaagggtga aaggatggtg ggcgctcatg tagcggcctt
    11941 cttcttcaga ccattcaaac atcggccagt caaccaccca aaggaagttg aacttatcat
    12001 tatcaatcaa gccaagctct ttagcaatac gtccacgaag ggcacccagt gttgcattag
    12061 ccacttcaag cgtatccgcc acaaagagaa ccaagtcctt atcttcaaga gcaagcgctg
    12121 ttgtcaattc ttcttggata ccagtcaaga acttggcaac tggtccgttt aattctccat
    12181 caaccacctt gacccaagca agacctttgg caccatactg tttggctact tccgtcatct
    12241 tgtcgatgtc tttacgtgaa tagttgtccg cagctccttt gaccacaatc gcttttacag
    12301 caggtgcttc tgaaaagact ttaaagtcta cacctttgac cacttctgtc aagtcctgaa
    12361 gcaacatgtc aaaacgagta tctggcttgt cagaaccgta aagagccata gcatcatcgt
    12421 atttcatatg agggaacggt agcgttactt cgatgccttt tgtttccttc atcacgcgcg
    12481 cgatcaagcc ttctgtaata tcttggattt cttgctcagt aaggaaggac gtttccaagt
    12541 cgacctgagt aaattcaggc tggcggtctc cacgcaagtc ctcgtcacgg aaacatttaa
    12601 cgatttggta gtaacggtca aaaccagcat tcatcaagag ctgtttcgtg atttgtggac
    12661 tttgaggaag agcgtaaaaa tgccccttat taacacgaga cggcactaaa taatcacgcg
    12721 ccccttcagg cgttgactta gaaaggaatg gtgtctccac gtcgataaac tccaactcat
    12781 ccaagtagtt gcggatagag tgggtcacct tggcacgaag tttaagattt tccaacattt
    12841 ctggacgacg aaggtcaagg taacggtaac gcaaacgtgt atcgtcattt gcctcaatgc
    12901 catccttaat ctcaaatggt gttgtcttag ctgtgttaag cacaataaga gctgtcacgt
    12961 ttaactcaac cgcaccagtt ggcaacttat cattggcttg ctcacgcgca gcgacctgac
    13021 cagtcacctc aataacaaat tcgctacgaa ggctttcagc tgttgccata acctctgcag
    13081 atactttttc agggttgata accaactgca tgattccttc acggtcacga agatcgataa
    13141 agatcaaacc accaaggtca cgacgacggc caacccatcc tttcaaggtt atttcttgtc
    13201 cgatgtgttc ctcacgaaca cgaccagcat acatactacg tttcattatt tctctcctct
    13261 tttattctgt tactatttta ccataaaagc gcaggctctt catgaaaatc atcagaaaag
    13321 tttgccagtc tttaaaagtc aggtgaaagc cctaaaaatt agcgctaatg ctcttcgaaa
    13381 atctcttcaa accacgtcag cgtcgcctta ccgtaggtat ggttactgac ttcgtcagtt
    13441 tcatctacaa cctcaaaacc atgttttgag ctgacttcgt cagtttcatc cacaacctca
    13501 aaacagtgtt ttgagcaacc tgcggctagc ttcctagttt gctctttgat tttcattgag
    13561 tataatacaa aaatccgatg aacttcaccg gactctttta ttttgaattt ttgcctgctt
    13621 tacgcttttc agcgatttcg gctgcctttc gaggcaagac aatttccgtt atgtaagccg
    13681 tcccaaaacg cagtacacct gcaataggag caaagacaac tgctagatag ttatagaaga
    13741 aatcgccttt gaaggcataa gctagcgctc caatgatgaa aaatagaacg actgcctgaa
    13801 tcactgctaa taaaattact cgtttcatgt gacctcctga ctctattata gcatgagaat
    13861 catcaaaaag ccgactaaat tattcaaagc gtgaagagaa atactgtaga ccagaccttt
    13921 tctgctaatg taagccaaac ccaaactaaa accaaggcta aaatagacaa aaaattgttg
    13981 cacatcacct ggaaaatgaa tcaaggcaaa tagaagacta gataccagaa gaaaaatcag
    14041 ggttcgttta ctattgtcct gcttaggaaa gagatagcgt gctaacatcc ctctaaaaac
    14101 aatctcttcc gtcaaaggag caaaaataac cacagcaaag aatgagaaaa gtggttgaga
    14161 caaggtcaag tctgtcgcta tttgttgatt tactgaagga tcatctggca agaagaattg
    14221 aacgaccaga gataagaacc aaaccaaggc aggaagccaa ataaatcggt taaagccgct
    14281 cttctcaata taaacaggag ctttctgata ccatttgtaa atgccgtaca catatactcc
    14341 agccaagacc acatagagta gagtaacagc atagggtgaa gcgcctaaag caagcgacgc
    14401 agtcgcgagc ccctgaataa agccatagat aaagaaaaag gatagaaggg ctagaagaaa
    14461 ccagccaagg tttttaagta gtttcataga taactccttt atttgaaata acgttttacc
    14521 ataggtaact gcatcacatt gatataaaca tggatggctc ctacaagcaa gaaagctagt
    14581 aactgaatct ctcctgtcaa gaaagaaatg ataataagaa aaatatataa ggctggtaag
    14641 acatattggt gtaattggaa taaaattcga aaactctgtt ccaaattagc ctgacgctcc
    14701 ccttcatcat aagaatttat atagttcaag acatcctttg gtgtagcgaa aaattccaaa
    14761 tcaaactgac gaacaatcgc aatggtttta aaaagagatt tttgagcgac taagaatacc
    14821 acaaagagta agaaagaaag gaaaaatgtt tgagggtttg tatgcaatat aatcacctca
    14881 cttaatgaaa taaaaatagc caatggaatc gctacacctg taatattaaa agcaatggtt
    14941 ccaaactcaa gattccgata catttgcaca taataggttt cattcagatc gtcatccatt
    15001 tcctcttgat acaaggaatg aaattttctg cttttcttta agaaattgaa agtcaaaaac
    15061 atactaatga aacctatcag taaacaaata gctgatatcc atggcatcaa ggcttttaca
    15121 tctaaaataa tttcgtggga ttcgacacgt gccttaaaca tccctacaaa catgcccaag
    15181 aaccccccca agacaataga catcaaaaat aacaatctac gtttcttttt catattcatt
    15241 ctcctttttc acttgctaga tttttggatt tcttttcaat ccattcaatt actgggatga
    15301 gagcaaagta gacccaaaca aattggtcgc tttgataggg attaaaccag cttaggtcca
    15361 tcccaatcag tagaaatacg ctgactaata aagctatgac cactacataa taaatcactt
    15421 tatacttgtt catcactcgt cctcctccaa acgaaatacc gattcgactg tttcgttgaa
    15481 aatttgagat attttcaggg caatgataat ggatggggtg tactcatccc gttctagtag
    15541 gctaatggtc tgtctggaaa cccctgccag tttggctagg tcggtttgat tgagaccatc
    15601 gcgagctcga agctctttta gacgattttt tagttgcatg ttacacacct actctccgtc
    15661 aaattcaacg gtttggatat cctcaatacg ttgcaacttg aatttttctt ttcccgtatt
    15721 atctacacgt cgtagcttta cccattcctc atcaacatcc acaacttccc agttatctgg
    15781 cccaatatac actcccgtta taattggttc ctttccaatc atttcttgta ataatctcga
    15841 catttctgcg tttcctttct cttttcgctc aagtcttttg attttcttct ctagtttctt
    15901 gattttttta gaattattag aataaaagaa aatcataaat agtataaatc ctagtaccca
    15961 cattataact cctttctgct tcctatttct taacttgaat tcattgtaac atatcttttt
    16021 ctttttgaca attatagttg tcaaaaagtt tatgattttt gtcattttgc aaaagaaaaa
    16081 ggtcaggagt aggttcctga ccactttatc tatcattaat actcttctaa aatctcttca
    16141 aaccacgtca gcttcacctt gccgtaggta tggttactga cttcgtcagt ttcatctaca
    16201 acctcaaaac agtgttttga gcaacctgcg gctagcttcc tagtttgctc tttgattttt
    16261 attgagtata aaatcctagt ttttcaaaga tttctgagaa gttttggctg attgtctcaa
    16321 gtgacacttg cacttcttct cgagtttggt tgttcttgac cgtcacttgt ccgctttcga
    16381 cttcgctctc tcctagggtg atgagggtct tagccacaaa gacatcggct gacttgaact
    16441 gagcttttag tttacggttg aggtaatcac gctctgcttt gaaaccttgt tggcgaagag
    16501 cctgtaccaa ttccaaggcc ttgatatttg ccccttcgcc caagactgcg atatagacat
    16561 ctagggcgtt ttcgataggg agggtcacgc cttgtttttc aaggatgaga agcaggcgct
    16621 ctacaccaag tccaaaacca aatccagcag tttcagggcc tccaaagtaa gcaaccaaac
    16681 catcgtagcg accacccgca cagactgtca agtcattgcc ctcaatctct gtgataaact
    16741 cgaaaatggt gtggttgtag tagtccagac cacgcaccat attggtatcg atgatgtaat
    16801 ctactccaag attttccaac atctgacgca cagcatcaaa atgagtttgg ctttcttcat
    16861 caagaaagtc caagatagac ggcgcattct ctactgccac cttgtcttct ttttccttag
    16921 agtccaagac acgaagagga ttttcctcca agcgacgttg gctatcctta gacaaggtct
    16981 ccttgagcgg tgtcaaatag tcaatcaagg cttggcggta ggctgcacgg ctctcaggat
    17041 ttccaagagt gttgaggtgc aatttgacac cttgaatacc gatttccttc aaaaaatggg
    17101 ctgccataac gattgtttcc acatcggtag ctggattgct agagccaaaa cactcaacac
    17161 caatctggtg gaattggcgc aagcgccctg cctgtggacg ctcataacgg aacataggtc
    17221 ccatgtagta gaacttgctt ggcttttgca cttctggggc gaagagttta ttttccacat
    17281 aggaacggac aacgggcgca gttccttctg gacggagggt aatatggcgg tcacccttgt
    17341 catagaaatc gtacatttcc ttggttacga tatccgttgt atctccgaca gagcggctga
    17401 taacctcgta atgctcgaaa ataggcgtac gcacttctgc gtagttgtag cgcttgaaaa
    17461 tttcacgggc aaagccctca acgtactgcc acttagcaga ctcagcaggt aaaatatcct
    17521 gcgttccttt tggtttttgt aatttcatag ggaatcctct ttaaacttaa tagtcttatt
    17581 ttaccataaa tagagggatt aaaacagtaa gaaaaaaatt aggatttaga tatcattttt
    17641 gagattaaga attgttaaaa aaatagctag caaggaaaaa ccaacaaata gcatccaagt
    17701 caactgcatg ttccatacgg ctactagtga aaaacaagct gttcccacag gtatggataa
    17761 ggtaaacaat agaccgaaaa aattactagt acgagctaga acctctggag ctagattttt
    17821 catgagcatg gcactaatct ttggttgaac tttaccagac acatacagag taaagaagag
    17881 aaatagcaaa ccaagcacga cttgattgaa taaattagcc aaaccaacta gactaaatcc
    17941 tacggtctcc cacatcatca atctaggcaa ggactgcttc ccaaaataat cattgcccgt
    18001 aaggctactg atgatgattg ctactaaaac acagaattga ttgataaata gtgcctctgt
    18061 ataagaaaaa ttcaagagag aatggctcaa aaagaagata ttataaattc cacccaaagc
    18121 gccacccaag gaattaataa gcaagacagc aaagagcata aaaccaaagt ttttctgtcc
    18181 acttttaaga aaaacgagac gtaaatttcg gtaaattgtt aggaaccggt ctttgataga
    18241 aagcttctca ttttttaagt tttcaccatc agcagatgac attgacaggc tcaatttgct
    18301 ttttcctaaa aagaggatag tggctgatac taggaaaaag caggcattga ttcccgcaac
    18361 gagagaaaaa ttattgaccg atagagctaa gagccagact ccgaaagctt gaccaccaat
    18421 agctgaaata taggtgatga actgtgaaaa agaataagcc tccatcaaat catcttcagc
    18481 tactttttcc ttaataagag gcatacgcag gccacctgca aaatcactga tgacatcact
    18541 aatgacattg atcaaacaca ggctagaaaa ggcaaagaga ctagcttgct gaacaactag
    18601 ggctgctaga aaaaatagaa ccgcctgaaa caaaccgcta tagaccatcc atttgacctt
    18661 gtccctcgtg taatctgccc gaatccctgc aaaaactgta aagagggtcg gaagaatcat
    18721 gacaatattc gccatagcaa cagcaaaaga tgcttgtggc aaggtcgatg catagacgat
    18781 aaagaccagg ttgaaaatcg aagcaccaaa agcattgaag aagcgtgaca aagtcaatag
    18841 gcgataagct tgatttctaa acaatagttt catagataat ctccctcctt gttctaagtt
    18901 ctacatcaag aatagcttta gtatagcact tatgaatttg cgaaacaatt cttttgaata
    18961 tatgcaacaa ataaaatgag caaaaaacga tagaactttt gtaatgacct gagaaagggc
    19021 aacagttcta tcgcttcaaa ttttttctta gtttgcagat attcaacatt cggtcgtatt
    19081 tctatagtat tcggcagatt tattacagcc aagcatctca aaaatacgga cagcatcctc
    19141 catctttttc tggccttcct tgactctacc ttgcttgcta tcaaggagac cttctgccca
    19201 cagataaaca attcggaaat aggtctcatt ttccttgtag aaatgctctt cgataacacg
    19261 tttaaaataa taggcattgg taaattcttc acactcaata ctagctaaaa agccattcaa
    19321 tagtatagta tgaaaaaggt ttcgattgcc agacatttcc attagaaaat cagatttacg
    19381 taccatttct cgtacatatc tagtaaaaag agaaacagat aaaaatggag aactgactga
    19441 aaataaattg agtttataga ttccccagat ctcggtagaa aacaaataat catgaaggac
    19501 ttttccttcc tctgctgtta actctaccct ttcatccatg ctcttcatat aagacttgat
    19561 cataatggca tttagaatat gtttctgttt gttgtgagaa tgggcatgct tttgatactc
    19621 cctgcgatat aagtcctcaa gaggtgctat attctttggt tccaagacat ctgtaatttc
    19681 ttttctcaac tcagaatctg tatcatactg gaaacctctc gccagaaaga ggatctcctc
    19741 cacactggca gatatatttt ccagagcaaa tagaaacttt tccaccgaaa gctcactctg
    19801 acctgtttca aaacgggaca acatagacgg cgaaaattgt cctccggttg cttgtctcag
    19861 tgagatattt cttgattctc gtaattgtct aaagactttt ccaatctgct ccatagactt
    19921 ccccttgatc ccgtattttc ttcattttat catatttttc agaaaattca tcaaaaactt
    19981 gccaaattgt cagaatcatg agaaaataga ggatatttat cacgtggagg gactgctatg
    20041 agagacgata tcaaaatcaa tgaccgtgct ttggccttgc aagaccaaat tatcgaaaaa
    20101 ctagagaaag tttttgatac agatgtggaa ttggatgttt acaatctagg tctgatttat
    20161 gaaatcaatc tggatgaaac ggggctctgc aagattgtca tgaccttcac cgatactgcc
    20221 tgtgattgcg ccgaaagcct gcctattgaa atcgtggcag gtctgaaaca aatcgagggt
    20281 atcaaagata tcaaggttga agttacctgg tcgcctgctt ggaaaatcac acgaatcagt
    20341 cgctatggcc gtattgccct tggactacca cctcgttaag caggccaatc acttttaaag
    20401 atgaaaaggg caaactagaa aactagccgc aggttgctca aaacactgtt ttgaagttat
    20461 ggatagaact gacgaagtca gctcaaaaca ctgttttgag gttgtggata gaactgacga
    20521 agtcagccca aaacactgtt ttgaggttgt ggatagaact gacgaagtca gtaaccatac
    20581 ctacggcaag gcgacgttga cgtgatttga agagattttc gagtatgagt ttatttttta
    20641 cctgacttgt ccatattcca gaagtctgtc acggctccgc gtgaagcaga tgatacgatg
    20701 tgggcatatt taccgaggac accacggctg taaagtggtg gcaaggttgt ttctgccttg
    20761 cgtttttcaa gttcttcttc ggatacggcc atggaaattt ctttggtatc ttggtcaacc
    20821 gtaacgatat cgccggtacg gagataggca attggtccac catcctgagc ttcaggagcg
    20881 atatgtccaa caaccagacc ataagtacca ccagagaaac gtccgtccgt caagagggcc
    20941 accttatctc cctgaccttt accaacaatc attgaagaaa gtgacagcat ctcaggcata
    21001 ccaggaccac ctttaggtcc aacaaaacga acaacgacta catcgccatc aacgatttca
    21061 tctgtcagga cggcctgaat cgcatcttct tctgagtcaa agaccttagc tggcccaacg
    21121 tgacgacgca ctttaacacc tgataccttg gcaactgcac cgtcaggagc aaggttcccg
    21181 ttcaagatga taagcggacc atccgcacgt tttggatttt caagtggcat gataactttt
    21241 tggcctggag tcaagtctgc aaagtcagcc aagttttcag ctacagtctt accagtacat
    21301 gtgatgcgat ctccgtgaag gaaaccattt gccaacaagt acttcataac cgcagggaca
    21361 ccaccgactt cgtagaggtc ttggaagaca tactgaccag atggtttcaa gtcggccaag
    21421 tgaggcacac gttcttgaat cgtattgaag tcctcaagtg acaagtcaac atttgcggca
    21481 tgggcaatgg cgagcaagtg aagagtggcg tttgtagaac caccgagagc catcgttaca
    21541 gtaatagcat cttcaaaggc ttcacgagtc aagatatctg atggtttgag accaagttcc
    21601 aacatcttaa caacagcacg tcctgctgct tcgatatctt ctttcttatc agctgattca
    21661 gctgggtgag aggatgaccc tggcaaactc atccctagaa cttcgatagc agttgccatg
    21721 gtattagcag tatacatacc accacaacca ccagggccag ggcaggcatt acattcaaga
    21781 cgtttcacgt cctcagctgt catgtcaccg tggttccatt ttccgatacc ttcaaagaca
    21841 gaaaccaagt cgatatcttt accatcaaga tttcccggtg caatagttcc accataggcg
    21901 aaaatagctg ggatatccat attagcaata gcaatcatag atccaggcat gttcttgtca
    21961 cagccaccga tagcgacgaa ggcatccacg ttgtgaccac tcatagccgc ctcgatggag
    22021 tccgcgatga tgtcacgaga tgttagagag aaacgcatac caggcgttcc catagcgatc
    22081 ccgtccgcta cggtaatggt tccaaactgt acaggccaag cgcctgcaga tttgacacct
    22141 tctttagcca gtttcccgaa atcatgcaag tgaatgttac atggtgtatt ttccgcccaa
    22201 gtcgaaatca ctcccacaat cgatgtttca aagtccttat ctgtcatacc agtcgcacga
    22261 agcatagcac ggttaggtga tttaaccatg ctgtcataga tgctactgcg gtgacgttta
    22321 tctaattcag tcatcttatc cctcccattt cagtttttac tattatagca caatttaaag
    22381 gcaatgaaca gaataaaatt cttgaatttt cagaaaattc tatacgcatg tgaaatattt
    22441 aaaattaaaa acaacaaagc ggattagtgc actttctgat gaccagaata tgctttttaa
    22501 tccgctttct ttaaataacg tactgtaatt tttacataaa ttctttcaga taagtgtatt
    22561 taacatctat cttgcattat aaatttctag aaccttctct tttatattcg attcactcaa
    22621 accatactca ttaagaagat aatccatttt ccctacttga ccgaatcttt cttgaacccc
    22681 catccgatga atttttgtta ttccatcatc agagaataat tcacataaag cactgccaat
    22741 tccacctatc tgattgtggt tttctacagt aaatatagtt tttccactta acattgtttt
    22801 tatctgttct ggtatcggtt tgattctaaa taaatctatc acacctattg aataacctaa
    22861 tttagacagt tcatctgcaa ctcgaatact tggagcaacc attatgccag aagcaacgat
    22921 tacaacatct tcaccatgtc ttaactcaat gtagccttta gaaaaatcct ctccaccttg
    22981 atacacagga actggagctt ttctaattgt tcgaatatat tttagtcctt ttaagtctaa
    23041 tgtctggttc aatatttcac gaaattggat atcatcagtt gcttcaaaaa tgatcgattt
    23101 aggaattaaa cgtaacaatc caatttcttc aaatggcata tgtgtcccac cattcatttc
    23161 tgcagttact cctgcatctg atccaatcac agtggcatcc aattgtgcgt atccaagaga
    23221 aataaataat tgatcaaata ctcttcgtga agaaaaagga ccaaatgtat gaagataagg
    23281 tctaaacccc tgaatagaca agcctgctgc aaggccgacc atttctgctt ccataatccc
    23341 aacattcaca taacggtctc caaagtcctt ttcaagatta tgagtagcca tcgaacttga
    23401 cagatcggct tctaagacta ctatatcaga atcactttga ttagcctcta aaaggaagtc
    23461 tctatataca tgccgtaatt ctttcgtact tctcatcatt ctgtttcctc caattcctga
    23521 cttaatcttt ctacaactga agttaacatt tgtctctcct ctacagtagg gcgaagatga
    23581 tgattggatt tcatttcttc cagctcttga accccttgac ctttaatagt atctaataca
    23641 atgcacttag gtgatgaatt atttgactgt tttaattgga caatcccttc ataaatttct
    23701 ctaatatctg aacccttgac cctaatggat tcaaatccaa atgctgaaaa tttttctacg
    23761 aaatcacctg gattacaaat atcctttgta aaaccatcta attgtttttt gttatcatca
    23821 acaaatacaa ttaagttgga taactgttga tgagaagcaa actgtatagc ctcccaacat
    23881 tgtccctcat ttaactcacc atctccaaca atagcataag tataaaaggg actctttctt
    23941 attctctgac catatgcaag tccagttgca acactaattc cttgtcctaa agagcccgtt
    24001 gtcatatcta tgcctggcgt tagatttcta tcaggatgag acggtaattt ggttccattt
    24061 gtatttaaag aatataagaa ttctttgtca aagaaaccat tcaaatagag tgtactgtat
    24121 agagctggtc ctccgtgacc ttttgataat atgaaataat ctctatctcg tgctgcaaat
    24181 atttctggag tcattggcat tatttcacca taaagcactg ctaaaacttc tacgatagac
    24241 agacttcctc cgtaatgtcc gaatccaaga tgattcaatg ttctaagagt atttaatcgg
    24301 atgttagtcg caaattttct taacccatct tctctatttt tacttaaaat catcccttat
    24361 tcctccgttg cagatggctt tttaataaag gatactccaa acataactgc tagaataaga
    24421 acaagaccaa tcacaatgcc tgcttgtgag ccaaattgat ttaacattcc taaaataatt
    24481 cctgatagac caaaatctgc atctgagaaa gttgatcctt ggaaaccaag tcctcccaaa
    24541 actggcatta aaaagactgg aagaaaactg attaaaatac cttgtaaaaa tgctccaata
    24601 gtggctccac gaacaccacc agatgcattc ccaatgacag acacctgcag tcgctccaca
    24661 gaagaaatga ggcacaacgc ctggtaagat aacaaccgtt cctgaagcaa tcataattgc
    24721 catacttact aaaccaccaa caaaactaga gataaatcca attagaactg cattgggtgc
    24781 ataagtataa acaatcggac aatccaaagc aggttttgaa ttaggtacaa gacgctctga
    24841 aataccttta aaggctggaa caatttcacc caaaataagg cgaacacctg ctaaaataac
    24901 aaatacccct gctgcaaatt gacctgctaa ttgtaaagca taaactagac cacttgtacc
    24961 actactgatt tctttttcta tatattctga ccctgcaaag atagctacaa taatgtaaat
    25021 aactgccatg gataaagtaa tactaacagt gctatcacgt aaaaaagcta aactctttgg
    25081 aaatttaatg tcctctgttg attttgattt gtcaccgata aggctaccag taaaaccact
    25141 caaccaatat cccaaagaac tgaaatgacc taaagccacc ttgtcatttc cagttaattg
    25201 aaccatatat ttttgcacaa atgctgggga aatactcata ataataccga gtgctaatcc
    25261 tcctagtaag ataagaggca agctagtaaa gccagcaact gataaaatga ccgcaatcat
    25321 acacgccata tatagagtgt ggtgccctgt taaaaagata tatttaaatc gagtaaaacg
    25381 agcgattaag atattgaaca ccatgcctgc aaacataatc attgcagtag ctgagccata
    25441 tgttgttaaa gctacagcta caattgcttc attattcggc acaacgccag ataaatgaaa
    25501 agcatgctca aacatggtac caaatggatt taaagaattt tgtacaattc ctgcaccacc
    25561 agatacaact aagaaaccaa caaaggtctt aattccacct ttaataatat caggtaattt
    25621 cttcttctga agaactaatc ctaaaattgc aattaaagct actaaaatag ctggtgtact
    25681 aacaatatct aatatgaact tcatcatgac gctagcctcc tatataagtc ctttttcttc
    25741 acaaagttta gtaattaatt ctcgtagttc atccatatca ataatactat ttaagatacg
    25801 aacatctcca agatgactag ctgaatcagc tagatcacga ccaacaatcc aaatatcagc
    25861 tgcatttgga tctgctccac ctaaatcata atgttcaact tctacatccg aaacattcaa
    25921 atcactcaat acagattcaa tattcatctg taccataaaa cttgaaccta atcctgaacc
    25981 acaagctgta ccaattttta acattatcta atcctcctgt ttaattatca ttttaatatc
    26041 atcatagttt tttgatgata ttaaagtttg aacatgattt ttatctctta aaattgttgt
    26101 taaatgtgac aaagccttta aatgactctc attatcaatg gctgcaatac aaatcaacaa
    26161 tcttacctct tgttctggat tatccaataa ataaatcggt tcttccaaaa ctaacattga
    26221 cattcctatt tcattcacac cttcatctgg ccgagcgtga ggaattgcta ctcccttccc
    26281 taaattaata aaaggtccaa actcttctac tttttgaatc attgcctcag ggtagttctc
    26341 cgttatctta tcttgatcca aaagcggttt agctgctaaa cgaatcgcct ccttccatcc
    26401 taatttttgc gaactaacct gataggtttc tttggtaata agttgttcta gcactggtac
    26461 aatttccttt ctatcatttt tttgataaag ataattcttt aacgccaatc ttaattccaa
    26521 ttcttgtgta ataattccat atcttttgac aatattcagg atttgttcaa tctcaaaatc
    26581 tccatactct aaattcggaa aatcttttaa cactagttct actagttgta ttgcttgctc
    26641 ttcagtcatc ataaccgaaa ctagataatt tggcttttct gtctccacct ttatggtaga
    26701 aaaaaccata tcatagtcac tactagcttt cacctgtaaa tcatcaatct ttgaggttcc
    26761 tataaactca atttgaggaa ataatgctaa tagattctct tttaacatca atgaagaact
    26821 aacaccatta ggacaaatga ttgctgcttt ataccatttt tgaggcaaag tatctgcttt
    26881 ctttaaataa cctccgaaat ggataacaaa atatgctgtt tcactatcag gtatgggatt
    26941 gtcaatagcg tccatcaagg gcatcaaaga atctttgact aattcaaata aatcaggata
    27001 atgttcttta acatgcaata catattcatt tgaactaggt aggccgaact ttaatctata
    27061 gtaagccggt ataaggtggc ggcgaagatt ttctctcaat ccttcccttt gtttaaaatg
    27121 taacaaagaa atatcttcca ttctacttat aatagcctct gttaattgat taaagtaaac
    27181 cggagcaaca tctacttcac cttcaaagca acttgataat aaaactgtga tatagcgata
    27241 atcatcctca gaaaacaccg tatctataat tcccaaatca accactgtat ccaataaaat
    27301 agtcgttata tcttgaataa caggagatac taatgtctct gaaagaccta ctctttcaac
    27361 atccctttga tacctacaca gaatgaatac taaaccgaaa aggtaaactt ttaattgatt
    27421 aacaataggt actagctgta gcttctcata ataatcttta actacctgat caatcaaatc
    27481 ataagttaat gaataccccc aactggataa aacataatcc aaaccccaaa tccctatgga
    27541 ggattccagc aactcactaa ccatttgaaa agctaagcgg tgcttattcc actctgaacc
    27601 gtgtaaagta taaccttttg ctctactgta ccctagctcc aaatcattat ctaacataat
    27661 ctttcttaat gattgaatat cagataaggt tgtattctta cttactttca aaaagtcttg
    27721 gtaatgacta ttcgatataa aatctaatcg gcaaaaagtg taaagataga ttaaagctaa
    27781 gcgagtcgac tttggtaaaa ccaattcatc cgacttaata atatctgtca aagactgctt
    27841 cgtacgattt gataaactat agcgaccttg ctttttatcc agcactatcc ctttattagc
    27901 tagataaggc actaaataat ctattccttc tttgacttcc tttataggta agctcacctt
    27961 aacagataat tcatataatg atagctcaca atgatccatc aaagtcatca aaataactag
    28021 tgctctataa tcaaacatct ctcttcc
//
LOCUS       DAVGJL010000013        27773 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.013, whole genome shotgun sequence.
ACCESSION   DAVGJL010000013 DAVGJL010000000
VERSION     DAVGJL010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27773
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.013"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            33..715
                     /locus_tag="VVE42_000483"
                     /pseudo
     CDS             33..715
                     /locus_tag="VVE42_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345255.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            complement(704..1507)
                     /locus_tag="VVE42_000484"
                     /pseudo
     CDS             complement(704..1507)
                     /locus_tag="VVE42_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            1502..1756
                     /locus_tag="VVE42_000485"
     CDS             1502..1756
                     /locus_tag="VVE42_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEU3072735.1"
                     /translation="MHRTTIPYFVSQEVYWKVRNIEAEAIRRNCERGAIFSGKIKYHE
                     DSQFKGDHYVECYAVLDNTVIARDRITVPIDPLCGKDFIE"
     gene            2001..3335
                     /gene="trmFO"
                     /locus_tag="VVE42_000486"
     CDS             2001..3335
                     /gene="trmFO"
                     /locus_tag="VVE42_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEU3072736.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVVNHPLIEVVRDEITELPTDVITVVATGPLTSDALAEKIHALNNGDGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAVRLFKEESEVIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            3419..4156
                     /locus_tag="VVE42_000487"
     CDS             3419..4156
                     /locus_tag="VVE42_000487"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEU3072737.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQSGNIKRVVFGENIGTTVSNNIEEKE"
     gene            4165..4722
                     /gene="frr"
                     /locus_tag="VVE42_000488"
     CDS             4165..4722
                     /gene="frr"
                     /locus_tag="VVE42_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEU3072738.1"
                     /translation="MANVIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRVHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDLGITPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            4782..5636
                     /gene="cvfB"
                     /locus_tag="VVE42_000489"
     CDS             4782..5636
                     /gene="cvfB"
                     /locus_tag="VVE42_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HEU3072739.1"
                     /translation="MNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKLRLTTLEVTATQDQFGWGRVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPELKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            5645..5860
                     /locus_tag="VVE42_000490"
     CDS             5645..5860
                     /locus_tag="VVE42_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEU3072740.1"
                     /translation="MRKSFYTWLMTERNPKSNSPKAILADLAFEEAAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDSIWQEYLEH"
     gene            5946..6914
                     /locus_tag="VVE42_000491"
     CDS             5946..6914
                     /locus_tag="VVE42_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEU3072741.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEESACEEARQVIQALMVLVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQEKLKNIHQIDFVHFSAKDVVRHPVVAQIIRAYEYSTEVAHD"
     gene            complement(6892..7071)
                     /locus_tag="VVE42_000492"
     CDS             complement(6892..7071)
                     /locus_tag="VVE42_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3072742.1"
                     /translation="MTQEDALIVISHIKVLSIVPNRCLKPLDKTFSLYNWIFLSQKYI
                     LLQANFLKISRVQLQ"
     gene            7107..7607
                     /locus_tag="VVE42_000493"
     CDS             7107..7607
                     /locus_tag="VVE42_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU3072743.1"
                     /translation="MIDQLSKYYSCRILTEKDIPSILSLYESNPLYFQHCPPEPNFAT
                     VKEDMLCLPEGKAKADKFFVGFWNGFDLVAVMDFVYAYPDEETVFIGLFMVDQAYQRK
                     GIGSHIVTEALAYFAKNFRKARLAYVKGNPQSQHFWEKQGFKSIGCEVKQELYTVVIV
                     EQSLED"
     gene            7610..7869
                     /locus_tag="VVE42_000494"
                     /pseudo
     CDS             7610..7869
                     /locus_tag="VVE42_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069408.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
     gene            complement(8236..8562)
                     /locus_tag="VVE42_000495"
     CDS             complement(8236..8562)
                     /locus_tag="VVE42_000495"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3072744.1"
                     /translation="MVDVAVDQGGVIETADRVTTHDEPVYEKHGVLHYAVANIPGAVA
                     RTSTIALTNVTLPYIEALAGKGFAQAISEDEGLRQGVTTYQGYLTSLPVAQGLNRDYT
                     DINDLV"
     gene            complement(8508..8921)
                     /locus_tag="VVE42_000496"
     CDS             complement(8508..8921)
                     /locus_tag="VVE42_000496"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEU3072745.1"
                     /translation="MGAHFLTKQAGGSGVLPGGVPGVPKGKVTIIGDGVVGTHAARIA
                     LGLGAQVTILDISAKRLSVLEEVFGSQIQTLISNSFNIEASMRDADVVIGAILIPGAK
                     APELVTDEMVKQMRPGSVSLTLLLTKVALSKQLTV"
     gene            complement(8922..9347)
                     /locus_tag="VVE42_000497"
     CDS             complement(8922..9347)
                     /locus_tag="VVE42_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEU3072746.1"
                     /translation="MLIGIPKEIKNNENRVALTPAGVHSLVSRGHRVLIETNAGLGSG
                     FTDADYQKQGAEIVATAGEAWAAELVVKVKEPLSSEYGYLRDVLLLFTYLHMAAAPEL
                     ADAMLAAKTTGIAYETVRDNQGQLPLLVPMSEVAGRMAV"
     gene            9524..10093
                     /locus_tag="VVE42_000498"
     CDS             9524..10093
                     /locus_tag="VVE42_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU3072747.1"
                     /translation="MESIFVKLAQYPSIETERLLLRPVTLDDAEAMFDYASDKGNTRY
                     TFPTNQSLEETKNNIAQFYLANPLGRWGIELKSNGQFIGTIDLHKIDSVLKKAAIGYI
                     INKKYWNQGLTTEANRAVIELAFEKIGMNKLTALHDKDNPASGKVMEKSGMRFSHAEP
                     YACMDQHEKDRIVTRVHYVLTKEDYFANK"
     gene            10161..10811
                     /locus_tag="VVE42_000499"
     CDS             10161..10811
                     /locus_tag="VVE42_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEU3072748.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQ
                     AEVAAVSKDLVSEKEVNKEEKEEPLEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQK
                     AGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGSGTASSISKEKKVNLNKA
                     SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGGKTIEKLKDYVTVD"
     gene            10795..13035
                     /locus_tag="VVE42_000500"
     CDS             10795..13035
                     /locus_tag="VVE42_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEU3072749.1"
                     /translation="MLQWIKNFSIPLIYLSFLLLWLYYAIFSASYLALLGFVFLLVCL
                     FIQFPWKSAGKVLIICGIFGFWFVFQNWQQSQASQNLADSVERVRILPDTIKVNGDSL
                     SFRGKSNGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFNYQAY
                     LKTQGIYQTLNIKTIQSLQKIGSWDIGENLSSLRRKAVVWIKTHFPDPMRNYMTGLLL
                     GHLDTDFEEMNELYSSLGIIHLFALSGMQVGFFMNGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFLTAGGVLSC
                     AYAFILTMTSKEGEGLKAVTSESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDL
                     TFLPLLSILFVLSFLYPVIQLNFIFEWLEGIIRLVSQVTSRPLVFGQPNAWFLILLLI
                     SLALVYDLRKNIKKLTVLCLLITGLFLLTKHPLENEITMLDVGQGESIFLRDVTGKTI
                     LIDVGGKAESYKKIEKWQEKMTTSNAQRTLIPYLKSRGVAKIDQLILTNTDKENVGDL
                     SEVTKAFHVGEILVSKDSLKQKEFVAELQTTQTKVRSMTVGENLPIFGSQLEVLSPRK
                     MGDGGHDDTLVLYGKFLDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGNKKSS
                     SPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGINSKVYRTDQQGAIRFKGLDSW
                     KIESVR"
     gene            13214..13402
                     /locus_tag="VVE42_000501"
     CDS             13214..13402
                     /locus_tag="VVE42_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3072750.1"
                     /translation="MTATKSYKYDWNTVLEYSTNYHDHQYAWIPEWSRYYSYSEYKVG
                     GGWNYARYEVINYYTGGY"
     gene            13434..14021
                     /locus_tag="VVE42_000502"
     CDS             13434..14021
                     /locus_tag="VVE42_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3072751.1"
                     /translation="MLNLTHVTLKTRQVILQDADFTFKKGRIYGLLAINGSGKTTLFR
                     AMSKLLPLSSGHIAVPPSLFYYESVEWLDGNLSGMDYLRLIKNIWKSDLNLRDEIAYW
                     EMADYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLAQI
                     DRQEQLVLLSSHYKEELVDICDRVVTIHQGQIEEV"
     gene            14025..15206
                     /locus_tag="VVE42_000503"
     CDS             14025..15206
                     /locus_tag="VVE42_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU3072752.1"
                     /translation="MKDVSLFLLKKVFKSRLNWIILLLFASVLGVTFYLNSQTANSVS
                     LERELETRLVDRERVINENEEKLSQMSDTSSEEYQFAKNNLDVQKNLLTRKTEILTLL
                     KEGRWKEAYYLQWQDEEKNYEFVSNDPTASSGLKMGVDRERKIYQALYPLNIKAHTLE
                     FPTHGIDQIVWILEVIIPSLFVVAIIFMLTQLFAERYQNHLDTAHLYPVSKVTFAMSS
                     LGVGVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYPIYSLVNQEVTIGKMQDVLFPS
                     LLLAFLAFIVIVEVVYLIAYFFKQKMPVLFLSLIGIVGLLFGIQTIQPLQRIAHLIPF
                     TYLRSVEILSGRLSKQIDNVDLNWSMGMVLLPCLIILLLVGILFIERWGSSRKKEVFK
                     V"
     misc_feature    15366..15497
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            15513..16043
                     /gene="infC"
                     /locus_tag="VVE42_000504"
     CDS             15513..16043
                     /gene="infC"
                     /locus_tag="VVE42_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEU3072753.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     gene            16076..16276
                     /gene="rpmI"
                     /locus_tag="VVE42_000505"
     CDS             16076..16276
                     /gene="rpmI"
                     /locus_tag="VVE42_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEU3072754.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            16328..16687
                     /gene="rplT"
                     /locus_tag="VVE42_000506"
     CDS             16328..16687
                     /gene="rplT"
                     /locus_tag="VVE42_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEU3072755.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            16745..17125
                     /locus_tag="VVE42_000507"
     CDS             16745..17125
                     /locus_tag="VVE42_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU3072756.1"
                     /translation="MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            17375..18175
                     /locus_tag="VVE42_000508"
     CDS             17375..18175
                     /locus_tag="VVE42_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEU3072757.1"
                     /translation="MNPTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHP
                     RAYLSLESRMACGMGACYACVLKVPESETVSQRVCEDGPVFRTGTVVL"
     gene            18186..19124
                     /locus_tag="VVE42_000509"
     CDS             18186..19124
                     /locus_tag="VVE42_000509"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEU3072758.1"
                     /translation="MTTNRLQVSLPGLDLKNPIIPASGCFGFGQEYAKYYDLDLLGSI
                     MIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAEKLPWLEREYPNLPIIA
                     NVAGFSKQEYAAVSHGISKATNVKAIELNISCPNVDHCNHGLLIGQDPDLAYDVVKAA
                     VEASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMINTLVGMRFDLKTRKPILANGT
                     GGMSGPAVFPVALKLIRQVAQTTDLPIIGMGGVDSAEAALEMYLAGASAIGVGTANFT
                     NPYACPDIIENLPKVMDKYGISSLEELRQEVKESLR"
     gene            19166..21331
                     /gene="lytB"
                     /locus_tag="VVE42_000510"
     CDS             19166..21331
                     /gene="lytB"
                     /locus_tag="VVE42_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-beta-N-acetylglucosaminidase"
                     /protein_id="HEU3072759.1"
                     /translation="MNLGEFWYNKINKNRGRRLMKKVRFIFLALLFFLASPEGAMASD
                     GTWQGKQYLKEDGSQAANEWVFDTHYQSWFYIKADANYADNEWLKQGDDYFYLKSGGY
                     MAKSEWVEDKGVFYYLDQDGKMKRNAWVGTSYVGATGAKVIEDWVYDSQYDAWFYIKA
                     DGQHAEKEWLQIKGKDYYFKSGGYLLTSQWINQAYVNASGAKVQQGWLFDKQYQSWFY
                     IKENGNYADKEWIFENGHYYYLKSGGYMAANEWIWDKESWFYLKFDGKIAEKEWVYDS
                     HSQAWYYFKSGGYMTANEWIWDKESWFYLKSDGKIAEKEWVYDSHSQAWYYFKSGGYM
                     TANEWIWDKESWFYLKSDGKMAEKEWVYDSHSQAWYYFKSGGYMTANEWIWDKESWFY
                     LKSDGKMAEKEWVYDSHSQAWYYFKSGGYMAKNETVDGYQLGSDGKWLGGKATNENAA
                     YYQVVPVTANVYDSDGEKLSYISQGSVVWLDKDRKSDDKRLAITISGLSGYMKTEDLQ
                     ALDASKDFIPYYESDGHRFYHYVAQNASIPVASHLSDMEVGKKYYSADGLHFDGFKLE
                     NPFLFKDLTEATNYSAEELDKVFSLLNINNSLLENKGATFKEAEEHYHINALYLLAHS
                     ALESNWGRSKIAKDKNNFFGITAYDTTPYLSAKTFDDVDKGILGATKWIKENYIDRGR
                     TFLGNKASGMNVEYASDPYWGEKIASVMMKINEKLGGKD"
     gene            complement(21392..23047)
                     /gene="pavA"
                     /locus_tag="VVE42_000511"
     CDS             complement(21392..23047)
                     /gene="pavA"
                     /locus_tag="VVE42_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HEU3072760.1"
                     /translation="MSFDGFFLHHIVEELRSELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEI
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTKSLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTETSFSPVPFANQVGEPFANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIALDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRRQREKIQKHKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            23152..23649
                     /gene="ybeY"
                     /locus_tag="VVE42_000512"
     CDS             23152..23649
                     /gene="ybeY"
                     /locus_tag="VVE42_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEU3072761.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAQKLGKEDKEMAVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPELEIAFDEEDLLENPELAEMMSEFDAYIGEL
                     FISIDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            23630..24025
                     /locus_tag="VVE42_000513"
     CDS             23630..24025
                     /locus_tag="VVE42_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEU3072762.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFALTGIFTAIKEERNMRKHAVTAL
                     VVILAGFVFQVSRIEWLFLLLSIFLVVAFEIINSAIENVVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            24042..24941
                     /gene="era"
                     /locus_tag="VVE42_000514"
     CDS             24042..24941
                     /gene="era"
                     /locus_tag="VVE42_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEU3072763.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKVPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPERFLVSEMVREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEREY"
     gene            24990..25814
                     /gene="mutM"
                     /locus_tag="VVE42_000515"
     CDS             24990..25814
                     /gene="mutM"
                     /locus_tag="VVE42_000515"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEU3072764.1"
                     /translation="MPELPEVETVCRGLEKLIIGKKISSIEIRYPKMIKTDLEEFQRE
                     LPSQIIESMGRRGKYLLFYLTDKVLISHLRMEGKYFYYPDQGPERKHAHVFFHFEDGG
                     TLVYEDVRKFGTMELLVPDLLDAYFISKKLGPEPSEQDFDLQVFQAALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQVHPARPSQTLTAEEATAIHDQTIAVLGQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKTGQECVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            25814..26419
                     /locus_tag="VVE42_000516"
     CDS             25814..26419
                     /locus_tag="VVE42_000516"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEU3072765.1"
                     /translation="MGKIIGITGGIASGKSTVTNFLRQQGFQAVDADAVVHQLQKPGG
                     RLFEALVQHFGQEIILENGELNRPLLASLIFSNPEEQKWSNQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDAQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            26406..27605
                     /locus_tag="VVE42_000517"
     CDS             26406..27605
                     /locus_tag="VVE42_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEU3072766.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSEQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILALYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDK
                     VGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRT
                     LLKVKEI"
     gene            27605..27754
                     /gene="rpmG"
                     /locus_tag="VVE42_000518"
     CDS             27605..27754
                     /gene="rpmG"
                     /locus_tag="VVE42_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU3072767.1"
                     /translation="MRVKINLKCSSCDSINYLTSKNSKTHPDKIEVLKYCPKERKVTL
                     HLESK"
BASE COUNT         8203 a         5142 c         6279 g         8149 t
ORIGIN      
        1 ttgcaaattt ttaagaaggg aggtgatctt gcatggactt tgaatatttt tataacagag
       61 aagcggaaag atttaacttc ttaaaagtac cggagatatt agttgataga gaagaatttc
      121 ggggcttatc agcagaagca attatccttt attccatact tcttaaacag acaggaatgt
      181 catttaagaa taactggata gacaaggaag gcagagtatt tatctatttt actgtcgaag
      241 aaattatgaa aagaagaaat atctcaaagc caactgccat aaaaacattt tttactaatt
      301 ttaagtgtag cataatacct ccaaagatga gcttgatgta aaaaaaggaa taggactgat
      361 cgaaagagta aggcttggac ttggtaagcc gaacatcatt tatgttaaag actttatgag
      421 tatatttcag gtaaaagaaa atgacttaca gaagtcaaaa aacttaactt cagaagtaaa
      481 agattttaac ctcagaagta aagaaaatga acttcaagag gttaagaacc ttgactctaa
      541 ctatatagag aataataaga gtaagtatag taagagagaa tatagttttg gtgaaaacgg
      601 acttggaaca tttcaaaatg tgtttttagc tgctgaagat atatcggatt tacaaatcat
      661 aatgaactca cagcttgaga attacattag acttcctgca aaactagaat cctagttcat
      721 gattgataat accagcactc aaattcattc gtaatccgaa gcgtttacga tgatttcaat
      781 aggttgttga aaacatttta aacgttttta ctttggcaaa gatgttctca accttgcttc
      841 tctccttaga tagcgcatgg ttacaggctt tatcttcagc tgttagcggc ttgagtttgc
      901 tggatttacg tggagtttgt gcttgaggat atatcttcat gagcccttga taaccactgt
      961 cagccaagat tttaccagct tgtccgatat ttctgcgact cattttgaac aacttcatat
     1021 catgacaata gttcacagtg atatccaaag aaacaattct cccttgactt gtgacaatcg
     1081 cttgagcctt catagcgtga aatttctttt taccagaatc attcgctaaa ttttttttag
     1141 ggcgattgat ttttacttcc gtcgcatcaa tcattaccgt gtcctcagaa ctgagaggag
     1201 ttcttgaaat cgtaacacca ctttgaacaa gagttacttc aacccattgg ctccgacgga
     1261 ttaagttgct ttcgtgaata ccaaaatcag ccgcaatttg ttcataagtt cgatattctc
     1321 gcacatattg aagagtggcc ataagaaggt cttctaggct taaattaggt tttcgtccac
     1381 cttttgcgtg tttaagttga taagctgttt ttaatacagc taatatctct tcaaaagtcg
     1441 tgcgctgaac accaacaaga cgcttaaatc gtgcatcagt tagttgttta cttgcttcat
     1501 cattcataga actactatac catattttgt ttcgcaggaa gtctattgga aagtaagaaa
     1561 tattgaagct gaggctatta gaagaaattg tgagcgtggt gctatttttt caggtaaaat
     1621 aaaatatcac gaagattctc agtttaaagg agatcactat gttgaatgtt atgctgtttt
     1681 agataatacg gttatagcaa gagatagaat aacagtccct atcgatccgt tatgtggaaa
     1741 agattttata gagtagcata taattgattc ttaactggaa tactcactat ctctttacat
     1801 caagaaaatg actaaacagg gaagtttgcc ttcttccctt tttttgttat actagtagaa
     1861 gaaaaaatag aaagatttgt gggagtgaaa aagccctgtg gactttttca gcctgagcca
     1921 agaaactcga aagcttgtaa gtctgattgg ctttttcaat gtgaatctta acttcacact
     1981 cccaaagagg tattagtgtc gtgtctcaat cttatatcaa tgttatcggt gctggtttgg
     2041 caggttctga agcagcttac caaatcgcag agcgtggtat tccagttaaa ctatatgaaa
     2101 tgcgtggtgt caagtctaca ccccagcata aaacagacaa ttttgctgag ttggtttgtt
     2161 ccaattcttt gcgtggggat gctttgacaa atgcagttgg tcttctcaag gaagaaatgc
     2221 gtcgcttggg ttctgttatc ttggaatctg ctgaggctac acgtgttcct gccggtggag
     2281 cccttgcggt ggatcgtgat ggtttctctc aaatggtgac cgaaaaagtg gtcaaccacc
     2341 ccttgattga agtggtgcgt gatgaaatta cagaattgcc aacagatgtt attacagttg
     2401 tggcgactgg tcccttgact agcgatgccc tggctgaaaa gattcatgcc cttaataatg
     2461 gcgatggctt ctatttctac gatgcagcag cgcctattat cgatgtcaac actatcgata
     2521 tgagtaaggt ctatctcaaa tctcgttatg ataagggaga agcagcctat ctcaatgctc
     2581 caatgaccaa gcaagagttt atggatttcc atgaagcctt ggtcaatgcg gaagaagcac
     2641 cgctcaattc ttttgaaaaa gaaaagtact ttgaaggatg tatgccaatc gaagttatgg
     2701 ccaaacgtgg cattaaaact atgctttatg gccctatgaa gccagtcggt cttgagtatc
     2761 cggacgacta cacaggacct cgtgatggag aatttaaaac accttatgcg gttgtgcaac
     2821 ttcgtcagga taatgcagct ggtagcctat ataatattgt tggtttccag acccacctca
     2881 aatggggaga acaaaagcgt gtcttccaaa tgattccggg tcttgaaaat gcggagtttg
     2941 tccgttatgg tgtcatgcat cgcaattctt acatggattc accaaatctt cttgagcaga
     3001 cttaccgttc caagaaacaa ccaaatctct tctttgctgg tcagatgacg ggggtggaag
     3061 gctatgttga gtcggcggct tcgggcttag ttgcgggaat taacgcagtt cgtctcttca
     3121 aggaagaaag cgaggttatt ttccccgaga cgacagcgat tggaagctta gctcattaca
     3181 ttacccatgc cgacagcaaa catttccaac caatgaatgt caattttggg atcatcaagg
     3241 agttggaagg cgagcgtatc cgtgataaga aggctcgtta tgaaaaaatt gcagagcgtg
     3301 cccttgccga cttagaggaa tttttgactg tctaattttt ttgaaagaat tgttcatgat
     3361 actataaaaa tcttagaaat tgtgataaaa taggtaggat gaaagaagga gagtgaaaat
     3421 ggcgaatccc aagtataaac gtattttaat caagttatca ggtgaagccc ttgccggtga
     3481 acgtggcgta gggattgata tccaaacagt tcaaacaatc gcaaaagaga ttcaagaagt
     3541 tcatagctta ggtatcgaaa ttgcccttgt tattggtgga ggaaatctct ggcgtggaga
     3601 acctgcagca gaagcaggta tggaccgtgt tcaggcagat tacactggaa tgcttgggac
     3661 tgttatgaat gctcttgtga tggcagattc attgcaacaa gttggggttg atacgcgtgt
     3721 acaaacagct attgctatgc aacaagtggc agagccttat gtccgtggac gtgcccttcg
     3781 tcaccttgaa aaaggccgta tcgttatctt tggtgctgga attggttcac catacttctc
     3841 gacagataca acagcggccc ttcgtgcagc tgaaatcgaa gcagatgcca tcctcatggc
     3901 taaaaatggc gtcgatggtg tgtacaatgc cgatcctaag aaggacaaga cagccgttaa
     3961 gtttgaagaa ttgacccacc gtgatgttat caacaaaggt cttcgtatca tggactcaac
     4021 agcctcaacc ctctcaatgg acaacgacat tgacttggtt gtcttcaaca tgaaccaatc
     4081 aggcaacatc aaacgtgtcg tatttggtga aaatatcgga acaacagttt caaataatat
     4141 cgaagaaaag gaataagaaa gaatatggct aacgtaatta ttgaaaaagc taaagagaga
     4201 atgacccagt ctcaccaatc acttgctcgt gaatttggtg gtatccgtgc tggtcgtgcc
     4261 aatgcaagct tgcttgaccg tgtacatgta gaatactatg gagtcgaaac tcctcttaac
     4321 caaatcgctt caattacgat tccagaagcg cgtgttttgt tggtaacacc atttgacaag
     4381 tcttcattga aagacatcga acgtgccttg aacgcttctg atcttggtat cacaccggct
     4441 aatgacggtt ctgtgattcg cttggttatc ccagctctta cagaagaaac tcgtcgtgac
     4501 cttgctaaag aagtgaagaa ggtcggcgaa aatgctaaag tggctgtccg caatatccgt
     4561 cgcgatgcta tggacgaagc taagaaacaa gaaaaagcaa aagaaatcac tgaagacgaa
     4621 ttgaagactc ttgaaaaaga tattcaaaaa gtaacagacg atgctgttaa acacatcgac
     4681 gacatgactg ctaacaaaga gaaagaactt ttggaagtct aaaaataaac agaaaaactc
     4741 agttggcatt gctggctgag ttttattcga aagaaggaaa tatgaataca aatcttgcaa
     4801 gttttatcgt tggactgatc atcgatgaaa atgaccgttt ttactttgtg caaaaggatg
     4861 gtcaaaccta tgctcttgct aaggaagagg gccaacatac agtaggggat acggtcaaag
     4921 gttttgcata cacggatatg aagcaaaaac tccgcctgac aaccttagaa gtgactgcca
     4981 ctcaggacca atttggttgg ggacgtgtca cagaggttcg taaggacttg ggtgtctttg
     5041 tggatacagg ccttcctgac aaggaaatcg ttgtgtcact cgatattctc cctgagctca
     5101 aggaactctg gcctaagaag ggcgaccaac tctacatccg tcttgaagtg gataagaaag
     5161 accgtatctg gggcctcttg gcttatcaag aagacttcca acgtctcgct cgtcctgcct
     5221 acaacaacat gcagaaccaa aactggccag ccattgttta ccgtctcaag ctgtcaggaa
     5281 cttttgttta cctaccagaa aataatatgc ttggttttat tcatcctagc gagcgttacg
     5341 cagagccacg tttggggcaa gtattagatg cgcgcgttat tggtttccgt gaagtggacc
     5401 gcactttgaa cctctccctc aaaccacgct cctttgaaat gttggaaaac gatgctcaga
     5461 tgattttgac ttatttggaa agcaatggcg gtttcatgac cttaaatgac aagtcatctc
     5521 cagacgacat caaggcaacc tttggcattt ctaaaggtca gttcaagaaa gctttaggtg
     5581 gtcttatgaa ggctggtaaa atcaagcagg accagtttgg tacagagttg atttagggag
     5641 gcttatgaga aaatcatttt acacttggct catgaccgag cgcaatccta aaagtaacag
     5701 tcccaaagca attttggcag acctcgcttt tgaagaggca gcttttccaa aacatacaga
     5761 tgattttgat gaggtcagtc gctttttgga ggagcatgcc agtttctctt ttaacctagg
     5821 agattttgac agcatttggc aggaatatct agaacactag catttattca ttgggtttgg
     5881 gctagtaatt tctccatccc tctgctataa taaaaagaaa taaaaggatt agagaggttc
     5941 tttatttgaa ggaacattca atagacattc aactgagtca tccagatgac ctgtttcatc
     6001 tttttggttc caatgagcgc catcttcgtt tgatggaaga agagctggat gtggtgattc
     6061 atgctcgtac ggagattgtc caggttttgg gagaagagtc tgcttgtgag gaagcccgtc
     6121 aggttattca agctctcatg gtcttggtga accgtgggat gaccgttggt acgccagatg
     6181 tggtgactgc aattagcatg gtcaaaaacg atgaaattga taagtttgtc gccctttacg
     6241 aagaagaaat tatcaaagat aatactggga aaccgatccg cgtcaaaacc ttaggtcaaa
     6301 aactttatgt ggacagtgtc aaacagcatg atgtgacctt tggaattggg cctgcaggga
     6361 cggggaagac ctttcttgca gttactttgg cagtgactgc ccttaaacgt gggcaagtca
     6421 agcggattat cttgacacgt ccagcggtgg aagcaggaga gagtctaggt tttcttccgg
     6481 gtgatcttaa ggagaaggta gatccttacc ttagacctgt ttatgatgcc ttgtatcaga
     6541 ttctggggaa agaccaaaca actcgactca tggagcgtga aattatcgag attgcgcccc
     6601 ttgcctatat gcgtggacgg accttggatg atgcctttgt cattctcgat gaggcgcaaa
     6661 atacgaccat catgcagatg aagatgttct tgactcgttt aggttttcat tctaagatga
     6721 ttgtcaatgg agatattagt cagattgacc tgccacgtaa tgtcaagtcc ggtttgattg
     6781 atgctcaaga gaaactcaag aacatccatc agattgactt tgttcatttt tcagccaagg
     6841 atgtggttcg ccatcctgtt gtcgctcaga ttatccgagc ctatgaatat tctactgaag
     6901 ttgcacacga ctgattttga ggaagttcgc ctgcaaaaga atatactttt gagagaggaa
     6961 aatccagttg tataggctaa aggttttatc caaaggtttg agacaacgat taggcacgat
     7021 ggaaagaact tttatgtggc tgatgacgat cagtgcatct tcctgtgtca taatcacagg
     7081 gcacaagaaa gtaggaattt gaaaagatga ttgaccaact atctaagtat tacagttgta
     7141 ggatactaac tgaaaaggat attccaagta ttttatcttt atatgaaagt aatcctctgt
     7201 attttcagca ttgtccacca gagccaaatt ttgcaactgt aaaagaggac atgctttgtc
     7261 tacctgaagg taaagctaag gctgataagt tttttgttgg attttggaat ggatttgacc
     7321 ttgtggctgt tatggatttt gtctatgcat atcctgatga ggagactgtt tttattggtt
     7381 tgtttatggt tgatcaagcc tatcagagga aagggattgg tagtcatatt gtgacagaag
     7441 cactagctta ttttgctaag aactttcgaa aggcacgttt ggcttatgtt aagggaaatc
     7501 cgcaatctca gcatttttgg gaaaagcagg gctttaaatc aattggatgc gaggttaagc
     7561 aagaactcta tacggttgtt atcgttgaac agagcctaga agattagaaa tggcatcaag
     7621 taagaactat ttggaatttg ttttggaaca attatcagga ttagatgatg tgacttaccg
     7681 ttccatgatg ggggagtata ttctttactt ccgcggcaag attattggcg gcatttatga
     7741 cgatcgcttt tagttaaacc cgtgcaagca gtcttagata agattgacca atcttctttt
     7801 gagtttccat acaaaggtgc caaagaaatg atttgagtgg aagaacttga taataagatg
     7861 tttctataag acctgatttt agctatgtat aaccaactgc caacgcccaa acctaaaaag
     7921 aaaaagcaag ggtgaacgaa gtaaaaaaga agtctgctaa ggccctgtct ttgcacgggt
     7981 aaaattttat atataaaaag aagctgggac taaagagctc agcttccttt ggtttatata
     8041 attgtcatta caagacgaag tggttgggcg aaactctgtt gactttattc aatttagagt
     8101 ttcttatgca caattgagtc tggaacgaaa gtctccagtt gcaaagtata cagtacaata
     8161 aaccaacgat gtaatagctg atgacacaaa gcacagtggg taggacttgc gaagtcaccc
     8221 ttttcttttc aaaatttata ctaaatcatt gatatcagtg tagtcacgat taagtccttg
     8281 agcaactggt aggctagtca agtaaccttg ataagtggtc acaccttgac gcaagccttc
     8341 atcttcagag attgcttgtg cgaatccttt gccagccaaa gcttcgatat aaggaagagt
     8401 gacattggtt agggcgatgg ttgaagtgcg ggcaaccgca ccagggatat tggcaacggc
     8461 atagtggaga acaccgtgtt tttcatagac gggttcatcg tgcgttgtca cacggtcagc
     8521 tgtttcgata acgccacctt ggtcaacagc aacgtcaacg atacagagcc tggacgcatt
     8581 tgtttgacca tctcatctgt caccaattcc ggtgcttttg caccagggat gagaatggct
     8641 ccaatcacca catcagcatc tctcatactt gcttcaatgt tgaatgaatt agatataaga
     8701 gtttgaattt gacttccaaa gacttcttct agaactgaga gacgcttggc actaatatct
     8761 aaaatagtca cttgagcacc aagaccaagg gcgatgcggg cagcatgtgt accgacgaca
     8821 ccatcaccga tgatagttac ttttcctttt ggaacacctg gtacaccacc aggtagaaca
     8881 ccagagccac cagcttgctt agtaaggaag tgagctccga tttaaacagc catacgacct
     8941 gcaacctcac tcataggaac gaggagcggt agttgtcctt gattgtcacg aacagtttca
     9001 taggcaattc ctgttgtttt tgctgctaac atagcatctg ctaattctgg agcagcggcc
     9061 atgtgcaagt aggtgaagag aagaagaacg tcgcgcaagt aaccgtattc agaacttaaa
     9121 ggttctttta ctttcacaac caactctgct gcccaagctt caccagcagt agcgacaatc
     9181 tcagctcctt gcttttgata gtcagcatca gtaaagccag aaccgagacc agcatttgtt
     9241 tcgataagga cacgatgacc acgactaact aagctatgaa cacctgcagg tgtgagggcg
     9301 acacggtttt cgttattttt aatttctttt gggattccga ttaacattga gataacctac
     9361 ctttcaattg acggtcttgt tttggttgtc acattccagt tcataaatca aaaatgtgac
     9421 ggtttcattg tatatgaaac cgcttcaaaa atcaagaaaa acttgtcatc caaatttttt
     9481 atgctagact agtgaaaatc aagctttgat tggttggaaa agtatggaat caatttttgt
     9541 aaaacttgcc cagtatccgt ctatagaaac ggagcgttta ttgcttagac ctgtaacttt
     9601 ggatgatgcg gaagcaatgt ttgactatgc ctcggacaag ggtaatacac gttacacttt
     9661 tccaaccaat caaagcttgg aagaaaccaa gaataacatt gctcagttct atttggctaa
     9721 tcccttggga cgttggggaa tagaactaaa aagcaatggt cagtttattg gaaccattga
     9781 cttgcacaag attgattctg ttcttaagaa ggcagctatt ggctacatta tcaataaaaa
     9841 gtattggaat caaggattaa cgacagaagc caatcgtgct gtgattgagc tagcttttga
     9901 gaagataggg atgaataagt tgactgccct tcacgataag gacaatcctg cgtcaggaaa
     9961 ggtcatggag aaatcaggca tgcgtttttc ccatgcagaa ccatatgctt gtatggacca
    10021 gcatgaaaaa gatcgaatcg ttacaagagt tcattatgtc ttgaccaagg aagactattt
    10081 tgcaaataaa taagcagttg aaaagaaatt tttcgactgt tttttcttcc tcttacgaat
    10141 aatctaagag aggagaaaat atggaagcaa ttatcgagaa aatcaaagag tataaaatca
    10201 tcgtcatctg tactggtctg ggcttgcttg taggcggatt tttcctgcta aagccagctc
    10261 cacaaacacc tgtaaaggaa acgaatttgc aggctgaagt tgcagctgtt tccaaggatt
    10321 tggtatccga aaaggaagtg aacaaggaag agaaggaaga accccttgaa caagatctaa
    10381 tcacagtaga tgtcaaaggt gctgtcaaat cgccagggat ttatgacttg cctgtaggta
    10441 gtcgagtcaa tgatgctgtt cagaaggctg gtggcttgac agagcaagca gacagcaagt
    10501 cgctcaatct agctcagaaa gttagtgatg aggctctggt ttacgttcct actaagggag
    10561 aagaagcagt tagtcaacag actggttcgg ggacagcttc ttcaataagc aaggaaaaga
    10621 aggtcaatct caacaaggcc agtctggaag aactcaagca ggtcaaggga ctgggaggaa
    10681 aacgagctca ggacattatc gaccatcgtg aggcaaatgg caagttcaag tcagtagacg
    10741 agctcaagaa ggtctctggc attggtggca aaacaataga aaagcttaaa gactatgtta
    10801 cagtggatta agaatttctc tattccccta atttacctga gttttctatt actttggctt
    10861 tattacgcta ttttctcagc atcttatctt gctttgttgg gctttgtttt tctgctagtc
    10921 tgtctcttta tccaatttcc gtggaaatct gctggtaaag ttctaataat ttgcggaatc
    10981 tttggatttt ggtttgtttt tcaaaattgg caacagagtc aagcgagtca aaatctggcg
    11041 gattctgttg aaagggtacg gattttgcct gatactatta aggttaatgg tgatagtcta
    11101 tcctttcgtg gcaagtctaa cggtcgtgct ttccaagtct attataaact ccagtccgag
    11161 gaggagaaag aagcctttca agctttaacc gacctgcatg agataggact agaagggaag
    11221 ctttcggagc cagaagggca gagaaatttt ggtggcttta attaccaagc ctatctgaag
    11281 actcagggaa tttaccagac tctcaatatc aaaacaatcc agtcacttca aaagattggc
    11341 agttgggata taggagaaaa cttgtccagt ttacgtcgaa aggctgtggt ttggattaag
    11401 acgcactttc cagaccctat gcgcaattac atgacaggac tcttgctggg acatctggac
    11461 accgactttg aggagatgaa tgagctttat tccagtctag gaattatcca cctctttgcc
    11521 ctatctggta tgcaggtagg ttttttcatg aatggattta agaaacttct cttgcgattg
    11581 ggcttgaccc aagaaaagtt gaaatggctg acttatccct tttcccttat ctatgcggga
    11641 ctaactggat tttcagcatc ggttattcgc agtctcttgc aaaagctact ggctcaacat
    11701 ggggttaagg gcttggataa ttttgccttg acggtgcttg tcctctttat tgtcatgcca
    11761 aactttttct tgacagcagg aggagtcttg tcctgcgctt atgcttttat cctgaccatg
    11821 accagcaaag aaggggaggg gctcaaggct gttactagtg aaagtctagt catctccttg
    11881 ggcatattgc ccattctatc cttctatttt gcggaatttc aaccttggtc tatccttttg
    11941 acctttgtct tttcctttct atttgacttg accttcttac cgctcttgtc tattttattt
    12001 gtcctttcct ttctctatcc agtcattcag ctgaacttta tctttgaatg gttggagggc
    12061 attattcgct tggtgtcaca ggtgacaagt agacctctgg tctttggaca acccaatgca
    12121 tggtttttaa tcctattgtt aatttccttg gctttggtct atgatttgag aaaaaacatt
    12181 aaaaagctaa cggtattgtg cttattgatt acagggctct ttctcctgac caagcatcca
    12241 ctggaaaatg aaatcaccat gctggatgtg gggcaaggag aaagtatttt cctacgggat
    12301 gtaactggga aaaccattct catagatgta ggtggtaagg cagaatctta taagaaaatc
    12361 gaaaaatggc aagaaaagat gacgaccagc aatgcccagc gaaccttgat tccctatctc
    12421 aaaagtcgag gagtagctaa gattgaccag ctaattttga ctaacacgga caaggagaat
    12481 gttggagatt tgtcagaggt gaccaaggct ttccatgtag gggagattct agtatcaaaa
    12541 gacagtctga aacagaagga atttgtggca gaactacaga cgactcaaac aaaggtgcgt
    12601 agtatgacag taggggagaa cttgcccatt tttggaagtc agttagaagt tctatctcca
    12661 aggaaaatgg gagatggagg acacgatgac accctagttc tgtatgggaa attcttggat
    12721 aagcaatttc tcttcacggg aaatttggag gagaaaggag agaaggactt gctgaagcac
    12781 tatccagact tgaaagtaaa tgttttgaaa gctagccaac atggcaataa aaaatcatca
    12841 agtccagcct ttctagaaaa actcaaacca gagcttactc ttatctcagt tggaaagagc
    12901 aatcgaatga aactccccca tcaggaaaca ttgacacgac tggaaggtat caatagcaaa
    12961 gtttatcgaa ctgaccagca aggagctata cgttttaagg ggttggatag ttggaaaatc
    13021 gaaagtgttc gataggaagg ataaatgttg tagattagtg aaataaacta aaaatttgtt
    13081 gcataataat gataaaaatg gtataatgaa aacgtattca atattgagga tataaaatca
    13141 ttaaaaatca gcaaaagttg ttttattagt tagtttataa tctattggtc ttcttcagtc
    13201 cagtgtatct gctgtgacag ccactaaaag ttacaagtat gattggaata cggttttgga
    13261 atatagtacc aactatcacg accatcagta tgcttggatt ccggaatggt ctcgttatta
    13321 cagctattct gagtataaag ttggcggagg ctggaactac gctcgttatg aggtcataaa
    13381 ctactatact ggaggttatt aatccttaaa gagtgagaaa aggagggctg gatatgttaa
    13441 atcttactca tgttacctta aaaacgcgac aagtcatctt gcaagatgcg gattttacct
    13501 ttaaaaaggg taggatttat ggccttcttg ctatcaatgg ctcgggaaag acgacactat
    13561 tccgagctat gagcaagttg cttcccctta gtagtggaca catcgcagtt cctccttctt
    13621 tgttttatta tgagagcgtt gaatggctgg atggaaactt aagtgggatg gactaccttc
    13681 gtctcataaa aaacatctgg aagtcagacc taaacttgag agatgaaatc gcctactggg
    13741 aaatggctga ctatatcagt cttcccatcc gcaagtattc cttagggatg aagcaacgct
    13801 tggtgattgc tatgtatttt ctcagtcagg ccaaatgctg gctcatggat gagattacaa
    13861 atggcttaga cgagtattat cgacagaagt tttttgatag gctagcacaa atcgatagac
    13921 aagaacagct ggttctttta agttcccact ataaggaaga gttggttgat atctgcgata
    13981 gagtagtaac cattcatcag gggcagatag aagaggttta gtttatgaaa gatgttagtc
    14041 tatttttatt gaaaaaagtt ttcaaaagtc gtttaaactg gattatctta cttttatttg
    14101 catctgtact cggtgttacc ttttatttaa atagtcagac tgcaaactca gtcagcttgg
    14161 agagagagtt ggaaactcgt cttgtagacc gtgagagagt catcaatgaa aatgaagaga
    14221 aactctccca aatgtctgat accagctcgg aggaatacca gtttgctaaa aataatttag
    14281 acgtgcaaaa aaatcttttg acgcgaaaga cagaaattct gactttatta aaagaagggc
    14341 gctggaaaga ggcctactat ctgcagtggc aagatgaaga gaagaattat gaatttgtat
    14401 caaatgaccc gactgctagc tctggcttaa aaatgggggt tgaccgcgaa cggaagattt
    14461 accaagccct gtatcccttg aacataaaag cacatacttt ggagtttccg acccacggga
    14521 ttgatcagat tgtctggatt ttagaggtta tcatcccaag cttgtttgtg gttgcgatta
    14581 tttttatgct aacacaacta tttgcagaaa gatatcaaaa tcatctggac acagctcact
    14641 tatatcctgt ttcaaaagtg acatttgcaa tgtcttctct tggagttgga gtgggctatg
    14701 taactgtgct gtttatcgga atctgtggct tttcttttct agtgggaagt ctgataagtg
    14761 gttttggaca gttagattat ccctacccga tttatagctt agtgaatcaa gaggtaacta
    14821 ttggaaagat gcaggatgta ttatttccta gcttgctctt agctttctta gcctttatcg
    14881 tcattgtgga agttgtgtac ttgattgctt actttttcaa gcaaaaaatg cctgtcctct
    14941 ttctttcact cattgggatt gttggcttat tgtttggcat ccaaacaatt cagcctcttc
    15001 aaaggattgc acatctgatt ccctttactt acttgcgttc agtggagatt ttatctggaa
    15061 gattatctaa gcagattgat aatgtcgatc taaattggag catggggatg gtcttacttc
    15121 cttgcctgat tatccttttg ttagtgggga ttctatttat cgaaagatgg ggaagttcac
    15181 gtaaaaagga agtttttaaa gtttagtttt cctataggga agctaaagaa aaactaacat
    15241 agggagagaa tcaacttgat tctctctttt tgattcgaaa accaaaccaa aatacaaaca
    15301 caaacttttc aaaaaataac tttttatctt gacaagagct agaaaacttg gtatcatata
    15361 aaagttgaga aaagcagaag tgagagcttc tcgccttgtg acattaagtt gcctggccct
    15421 acggatgaaa agtttcgaag aaacgctatc ataacgtgcg ggcttgtata tttacgagtc
    15481 cgctattgtt tttctctaat aaaacaaaag aggtgaaaac catagcaaag caagacttat
    15541 tcatcaatga tgagattcgt gtacgtgaag ttcgcttgat tggtcttgag ggagaacagc
    15601 taggcatcaa gccactcagt gaagcgcaag ctttggctga taacgctaat gttgacctag
    15661 tattgattca accccaagcc aaaccgcctg ttgcaaaaat tatggactac ggtaagttca
    15721 aatttgagta ccagaagaag caaaaagaac aacgtaaaaa acaaagcgtt gttactgtga
    15781 aagaagttcg tctaagtcca actattgaca agggtgactt tgatacaaaa cttcgtaatg
    15841 cacgtaagtt ccttgaaaaa ggaaataaag ttaaggtatc tattcgcttt aagggtcgta
    15901 tgattaccca taaagagatt ggtgcaaaag ttttagccga gtttgctgaa gcaactcaag
    15961 atattgccat catcgaacaa cgtgctaaaa tggatggacg tcaaatgttc atgcaattgg
    16021 cgccagcgac tgacaaaaaa taattgtcag aaagttaaat aaaggagaaa aaatcatgcc
    16081 aaaacaaaaa acacaccgcg catcagctaa acgtttcaaa cgtacaggtt ctggtggact
    16141 taaacgtttc cgtgcttaca cttctcaccg tttccacgga aaaactaaga aacaacgtcg
    16201 tcatcttcgt aaagcatcta tggtgcattc aggagattac aaacgtatca aagcaatgct
    16261 tactcgcttg aaataatagc ttgactgtaa gtaaacaatt ctaggaaata tttggaggaa
    16321 ataaaacatg gcacgtgtta aaggtggcgt tgtatcacgc aaacgtcgta aacgtattct
    16381 taaattagca aaaggttact atggagctaa acacatcttg ttccgtactg caaaagaaca
    16441 agtaatgaac tcttactact atgcataccg tgaccgtcgt cagaaaaaac gtgacttccg
    16501 caaattgtgg attactcgta tcaatgcggc agctcgtatg aacggacttt catactcaca
    16561 attgatgcat ggtttgaaat tggctgagat tgaagttaac cgtaaaatgc ttgctgactt
    16621 ggctgttaac gatgcagtag ctttcacagc tcttgcagat gcagctaaag caaaacttgg
    16681 taaataacaa ctgatgagac tggaacagga ttgtccagtc tttttaaaaa taaaggagaa
    16741 agatatggct tcaaaaatgc tacacacttg cttacgagta gaaaatcttg aaaaatcaat
    16801 cgcattttat caagatgctt ttggttttaa agaattgcgt cgcagagatt ttccagacca
    16861 tgccttcacg attgtctatc taggtcttga gggtgatgac tatgagttgg agttgactta
    16921 taactacgat cacggtccgt atgtggttgg tgatgggttt gcccatatcg ccctcagtac
    16981 acctgatctt gaggcacttc atcaagaaca cagtgcaaaa ggctatgaag tgactgagcc
    17041 aaatggtcta ccaggaacta caccaaacta ttactttgtc aaagaccctg atggatataa
    17101 ggtcgaagtc attcgtgaaa aataatttcg tgcaagttaa gtagaatatt cgttttctac
    17161 ttgacttttt ttagctgaaa gagtatacta ataaaaattc aacctttaag gggagtccag
    17221 agagactcac aaggtgtcag ataaaagaat agtgcaattt tctagaggag actttttgag
    17281 tgtgctctct tgtgttgtac gattttaact gaggccttgc actagcaagg tcttttcttt
    17341 atctggtccc cttaaaattt aaggaggaaa agttatgaat cccacatgta agaagcgttt
    17401 gggtgtcatt cggttggaaa ccatgaaggt ggttgcacaa gaggaaatcg cgccagcaat
    17461 ctttgaatta gtcctagaag gagaaatggt tgaagccatg cgagcaggcc aatttcttca
    17521 tctgcgtgta ccggacgatg cccatctctt acgtcgtcct atttcaattt cgtctattga
    17581 caaggcaaac aagcagtgtc acctcattta tcggattgag ggagctggga ctgcaatttt
    17641 ttcaacctta agtcagggag acactcttga tgtgatgggg cctcagggaa atggttttga
    17701 cttgtctgac cttgatgagc agaatcaggt tctccttgtt ggtggtggga ttggtgttcc
    17761 acccttgctt gaggtggcca aggaattgca tgaacgtgga gtgaaagtag tgacagtcct
    17821 cggttttgct aataaggatg ctgttatttt gaaaacggaa ttggctcagt atggtcaggt
    17881 ctttgtaacg acagatgatg gttcttatgg catcaaggga aatgtttccg ttgttatcaa
    17941 tgatttagat agtcagtttg atgctgttta ctcgtgtggg gctccaggaa tgatgaagta
    18001 tatcaatcaa acctttgatg atcacccaag agcctattta tctctggaat ctcgtatggc
    18061 ttgtgggatg ggagcttgct atgcctgtgt tctaaaagta ccagaaagcg agacggtcag
    18121 ccaacgcgtc tgtgaagatg gtcctgtttt ccgcacagga acagttgtat tataaggaga
    18181 aaattatgac tacaaatcga ttacaagtgt ctctacctgg tttggatttg aaaaatccga
    18241 ttattccagc atcaggctgt tttggctttg gacaagagta tgccaagtac tatgatttag
    18301 accttttagg ttctattatg atcaaggcga caacccttga accacgtttt gggaatccaa
    18361 ctccaagagt ggcagagacg cctgctggta tgctcaatgc aattggcttg caaaatcctg
    18421 gtttagaggt tgttttggct gaaaagctac cttggctgga aagagaatat ccaaatcttc
    18481 ctattattgc caatgtagct ggtttttcaa aacaagagta tgcagctgtt tctcatggga
    18541 tttccaaggc aactaatgta aaagctatcg agctcaatat ttcttgtccc aatgttgacc
    18601 actgtaatca tggacttttg attggtcaag atccagattt ggcttatgat gtggtgaaag
    18661 cagctgtgga agcctcagaa gtgccagttt atgtcaaatt aaccccgagt gtgaccgata
    18721 tcgttactgt cgcaaaagct gcagaagatg cgggagcaag tggcttgacc atgatcaata
    18781 ctctggttgg aatgcgcttt gacctcaaaa ccagaaaacc aatcttggcc aatggaacag
    18841 gtggaatgtc aggtccagca gtctttccag tagccctcaa actcatccgc caagtagccc
    18901 aaacaacaga cctgcctatc attggaatgg ggggagtgga ttcggctgaa gctgccctag
    18961 aaatgtatct ggctggggca tctgctatcg gagttggaac agctaacttt accaatcctt
    19021 atgcctgccc tgacatcatc gaaaatttac caaaagtcat ggataaatac ggtattagca
    19081 gtctggaaga actccgtcag gaagtaaaag agtctctgag gtaaactgca atcaatctgt
    19141 tcttgatttt ttattagttt gtaatatgaa tttaggagaa ttttggtaca ataaaataaa
    19201 taagaacaga ggaagaaggt taatgaagaa agtaagattt atttttttag ctctgctatt
    19261 tttcttagct agtccagagg gtgcaatggc tagtgatggt acttggcaag gaaaacagta
    19321 tctgaaagaa gatggcagtc aagcagcgaa tgagtgggtt tttgatactc attatcaatc
    19381 ttggttctat ataaaagcag atgctaacta tgctgataat gaatggctaa agcaaggtga
    19441 cgactatttt tacctcaaat ctggtggcta tatggccaaa tcagaatggg tagaagacaa
    19501 gggagtcttt tattatcttg accaagatgg aaagatgaaa agaaatgctt gggtaggaac
    19561 ttcctatgtt ggtgcaacag gtgccaaagt aatagaagac tgggtctatg attctcaata
    19621 cgatgcttgg ttttatatca aagcagatgg acagcacgca gagaaagaat ggctccaaat
    19681 taaagggaag gactattatt tcaaatccgg tggttatcta ctgacaagtc agtggattaa
    19741 tcaagcttat gtgaatgcta gtggtgccaa agtgcagcaa ggttggcttt ttgacaaaca
    19801 ataccaatct tggttttaca tcaaagaaaa tggaaactat gctgataaag aatggatttt
    19861 cgagaatggt cactattatt atctaaaatc cggtggctac atggcagcca atgaatggat
    19921 ttgggataag gaatcttggt tttatctcaa atttgatggg aaaatagctg aaaaagaatg
    19981 ggtctacgat tctcatagtc aagcctggta ctacttcaaa tccggtggtt acatgacagc
    20041 caatgaatgg atttgggata aagaatcttg gttttacctc aaatctgatg ggaaaatagc
    20101 tgaaaaagaa tgggtctacg attctcatag tcaagcttgg tactacttca aatccggtgg
    20161 ttacatgaca gccaatgaat ggatttggga taaggaatct tggttttacc tcaagtctga
    20221 tggcaaaatg gctgaaaaag aatgggtcta cgattctcat agtcaagcct ggtactactt
    20281 caaatctggt ggctacatga cagccaatga atggatttgg gataaggaat cttggtttta
    20341 cctcaagtct gatggcaaaa tggctgaaaa agaatgggtc tacgattctc atagtcaagc
    20401 ctggtactac ttcaaatctg gtggctacat ggcgaaaaat gagacagtag atggttatca
    20461 gcttggaagc gatggtaaat ggcttggagg aaaagctaca aatgaaaatg ctgcttacta
    20521 tcaagtagtg cctgtcacag ccaatgttta tgattcagat ggtgaaaagc tttcctatat
    20581 atcgcaaggt agtgtcgtat ggctagataa ggatagaaaa agtgatgaca agcgcttggc
    20641 tattactatt tctggtttgt caggctatat gaaaacagaa gatttacaag cgctagatgc
    20701 tagtaaggac tttatccctt attatgagag tgatggccac cgtttttatc actatgtggc
    20761 tcagaatgct agtatcccag tagcttctca tctttctgat atggaagtag gcaagaaata
    20821 ttattcggca gatggcctgc attttgatgg ttttaagctt gagaatccct tccttttcaa
    20881 agatttaaca gaggctacaa actacagtgc tgaagaattg gataaggtat ttagtttgct
    20941 aaacattaac aatagccttt tggagaacaa gggcgctact tttaaggaag ccgaagaaca
    21001 ttaccatatc aatgctcttt atctccttgc ccatagtgcc ctagaaagta actggggaag
    21061 aagtaaaatt gccaaagata agaataattt ctttggcatt acagcctatg atacgacccc
    21121 ttacctttct gctaagacat ttgatgatgt ggataaggga attttaggtg caaccaagtg
    21181 gattaaggaa aattatatcg ataggggaag aactttcctt ggaaacaagg cttctggtat
    21241 gaatgtggaa tatgcttcag acccttattg gggcgaaaaa attgctagtg tgatgatgaa
    21301 aatcaatgag aagctaggtg gcaaagatta gtactataag tgaatatgat ttgagtgaat
    21361 agtaagttaa aaatcctgat ttcaagtaaa atcaggattt tttcatggat gcaatttttt
    21421 tggagtctgg tgtgacgcgg agggtctttt gtcctgtgta agtgacaaag ccgggttttc
    21481 caccagttgg tttattgagt tttttgactt caatcatatc tacctgcacc agattcgaca
    21541 ggcgcccttg agagaagtag gcagctaact ctgctgcgtc tgtcttgact gcatcagatg
    21601 ggtcaagatt tcctgagatg acaacatggc ttccaggaat gtccttagca tggaaccaaa
    21661 gttcctcctt gcgggccatt ttaaaggtca attcctcatt ttgaagattg tttcgtccga
    21721 catagatgat ggttttgcca tcgcttgcta gatattgttc tagttttttg tgtttctgga
    21781 ttttctcccg ttgtcttctg cggataaaac ctgtttgaat caattcttca cggatttcag
    21841 cgatttcttc cagtccagct tggttgagga cggtttctac actttccaga tagagaatag
    21901 tggctttggt ttcttcaatc aaatcagtca agtatttgac agcttctttg agtttctgat
    21961 accgtttaaa atagcgttgg gcattctggt tgggagtcag agccttatca agcgcaatca
    22021 tgataggttg gttggtatag tagttgtcta ggataacctg gtcttggtcg ttaggcactt
    22081 ggtggaggaa ggttgtcagc aattctcctt tttgacgaaa ttcttcagcg ttgtctgtcg
    22141 ccagtaactc tttttcctgt tttttgagtt tgtgtcggtt tttctgaagt tcattttcaa
    22201 cacgacgaat cagttcactg gcctgctgtt tgacgcggtc gcgctcagcc ttatccttat
    22261 agtaggtgtc caacaaatca gaaagatttg caaaaggctc tcccacctga tttgcaaaag
    22321 gaactggact gaaggaagtc tcagtcaagc atggcttggt ttcttgattg aaaaaatttc
    22381 ggaaagcgga aagtttttca ctaaccagta tcctttccaa ttcatttgcc gtatcgcgtc
    22441 ccagaccttg aaagaggctt tgaagatttt ttgctgttag ttcttgggtt tgcaggattt
    22501 caaagagctt ttcatccttg atagtaaaag gattgagaga ttttgtactt ggcggagcga
    22561 tataggtcga tcctggaagt aaggtgcggt agctattttg tgaaaagccg acgtgtttga
    22621 taacttcgag gattttatga ctgcttttat cgaccagtag aatattactg tgtttcccca
    22681 taatttcgat aatcaaggta gcctggatat ggtctccaat ctcgttttta ttggaaactg
    22741 taatttccac aatacggtca ttttccactt gctcaatcga ctcaatcagg gccccctgca
    22801 aatactttct caaaaccatg ataaaggtag aaggttgggc tggattttca aaagtcgttt
    22861 gggttagctg aatgcgtcca aaaactggat gggcagaaag gagcaggcga tggctttggc
    22921 gattgctgcg gatttgcaag accaactctt gttcaaaagg ctgattgatt ttctggatgc
    22981 gaccattcac taattcgctt cgcaattcct caactatgtg gtgtaaaaaa aatccgtcaa
    23041 atgacatcgt tctctccttg tgattgtatt ccatagtatt atatcaaaaa ggtagaataa
    23101 aatcatggaa atgtggtata ataaagccaa gtaaagagaa acgagaagca catgtatatt
    23161 gaaatggtag atgaaactgg tcaagtttca aaagaaatgt tgcaacaaac ccaagaaatt
    23221 ttggaatttg cagcccaaaa attaggaaaa gaagacaagg agatggcagt cacttttgtg
    23281 accaatgagc gtagtcatga acttaatctg gagtaccgtg acaccgaccg tccgacagat
    23341 gtcatcagcc ttgagtataa accagaattg gaaattgcct ttgacgaaga ggatttgctt
    23401 gaaaatccag aattggcaga gatgatgtct gagtttgatg cctatattgg ggaattgttc
    23461 atctctatcg ataaggctca tgagcaggct gaagaatatg gtcacagctt tgagcgtgag
    23521 atgggcttct tggcagtaca cggcttttta catattaacg gctatgatca ctacactccg
    23581 gaagaagaag cggagatgtt cggtttacaa gaagaaattt tgacagccta tggactcaca
    23641 agacaataaa cgaaaatgga aaaatcgtga cttgatatcc agtttagaat ttgctttgac
    23701 aggtattttt actgctatca aggaagaacg caatatgcga aaacacgcag tgacggctct
    23761 agtggtcatc cttgcaggtt ttgtttttca ggtgtcacga atcgaatggc tctttctcct
    23821 attgagtatt ttcttggtag tagcctttga gattatcaac tctgctattg aaaatgtggt
    23881 agatttggcc agtcactatc acttttccat gctggctaaa aatgccaagg atatggcggc
    23941 cggcgcggta ttagtggttt ctcttttcgc agccttaaca ggcgcattga tttttctccc
    24001 acgaatctgg gatttattat tttaaacagt aagaggaaat tatgactttt aaatcaggct
    24061 ttgtagccat tttaggacgt cccaatgttg ggaagtcaac ctttttaaat cacgttatgg
    24121 ggcaaaagat tgccatcatg agtgacaagg cgcagacaac gcgcaataaa atcatgggaa
    24181 tttacacgac tgataaggag caaattgtct ttatcgacac accagggatt cacaagccta
    24241 aaacagctct cggagatttc atggttgagt ctgcctacag tacccttcgc gaagtggaca
    24301 ccgttctttt catggtgcct gctgatgaag cgcgtggtaa gggggacgat atgattatcg
    24361 agcgtctcaa ggctgccaag gttcctgtga ttttggtggt gaataaaatc gataaggtcc
    24421 atccagacca gctcttgtct cagattgatg acttccgtaa tcaaatggac tttaaggaaa
    24481 ttgttccaat ctcagccctt cagggaaata acgtgtctcg tctagtggat attttgagtg
    24541 aaaatctgga tgaaggtttc caatatttcc cgtctgatca aatcacagac catccagaac
    24601 gtttcttggt ttcagaaatg gttcgcgaga aagtcttgca cctaactcgt gaagagattc
    24661 cgcattctgt agcagtagtt gttgactcta tgaaacgaga cgaagagaca gacaaggttc
    24721 acatccgtgc aaccatcatg gtcgagcgcg atagccaaaa agggattatc atcggtaaag
    24781 gtggcgctat gcttaagaaa atcggtagca tggcccgtcg tgatatcgaa ctcatgctag
    24841 gagacaaggt cttcctagaa acctgggtca aggtcaagaa aaactggcgc gataaaaagc
    24901 tagatttggc tgactttggc tataatgaaa gagaatacta agtagaggta ggctcatgcc
    24961 tgcttcttgt ttttacagaa ggaggactta tgcctgaatt acctgaggtt gaaaccgttt
    25021 gtcgtggctt agaaaaattg attataggaa agaagatttc gagtatagaa attcgctacc
    25081 ccaagatgat taagacggat ttggaagagt ttcaaaggga attgcctagt cagattatcg
    25141 agtcaatggg acgtcgtgga aaatatttgc ttttttatct gacagacaag gtcttgattt
    25201 cccatttgcg gatggagggc aagtattttt actatccaga ccaaggacct gaacgcaagc
    25261 atgcccatgt tttctttcat tttgaagatg gtggcacgct tgtttatgag gatgttcgca
    25321 agtttggaac catggaactc ttggtgcctg accttttaga cgcctacttt atttctaaaa
    25381 aattaggtcc tgaaccaagc gaacaagact ttgatttaca ggtctttcaa gctgcccttg
    25441 ccaagtccaa aaagcctatc aaatcccatc tcctagacca gaccttggta gctggacttg
    25501 gcaatatcta tgtggatgag gttctctggc gagctcaggt tcatccagct agaccttccc
    25561 agactttgac agcagaagaa gcgactgcca ttcatgacca gaccattgct gttttgggcc
    25621 aggctgttga aaaaggtggc tccaccattc ggacctatac caatgccttt ggggaagatg
    25681 gaagcatgca ggactttcat caggtctatg ataagactgg tcaagaatgt gtacgctgtg
    25741 gtaccatcat tgagaaaatt caactaggcg gacgtggaac ccacttttgt ccaaactgtc
    25801 aaaggaggga ctgatgggaa aaatcatcgg aatcactggg ggaattgcct caggtaagtc
    25861 aactgtgaca aattttctaa gacagcaagg ctttcaagca gtggatgccg acgcagtcgt
    25921 ccaccaacta cagaaacctg gtggtcgtct gtttgaggct ctagtacagc actttgggca
    25981 agaaatcatt cttgaaaacg gagaactcaa tcgccctctc ctagctagtc tcatcttttc
    26041 aaatcctgaa gagcaaaaat ggtctaatca aattcaaggg gagattatcc gtgaggaact
    26101 ggctactttg agagaacagt tggctcagac agaagagatt ttcttcatgg atattcccct
    26161 actttttgag caggactaca gcgattggtt tgctgagact tggttggtct atgtggaccg
    26221 agatgcccaa gtagaacgct taatgaaaag ggaccagttg tccaaagatg aagctgagtc
    26281 tcgtctggca gcccagtggc ctttagaaaa aaagaaagat ttggccagcc aggttcttga
    26341 taataatggc aatcagaacc agcttcttaa tcaagtgcat atccttcttg agggaggtag
    26401 gcaagatgac agagattaac tggaaggata atctgcgcat tgcctggttt ggtaattttc
    26461 tgacaggagc cagtatttct ttggttgtac cttttatgcc catcttcgtg gaaaatctag
    26521 gtgtagggag tgagcaagtc gctttttatg caggcttagc aatttctgtc tctgctattt
    26581 ccgcggcgct cttttctcct atttggggta ttcttgctga caaatacggc cgaaaaccca
    26641 tgatgattcg ggcaggtctt gctatgacta tcactatggg aggcttggcc tttgtcccaa
    26701 atatctattg gttaatcttt cttcgtttac taaacggtgt atttgcaggt tttgttccta
    26761 atgcaacggc actgatagcc agtcaggttc caaaggagaa atcaggctct gccttaggta
    26821 ctttgtctac aggcgtagtt gcaggtactc taactggtcc ctttattggt ggctttatcg
    26881 cagaattatt tggcattcgt acagttttct tactggttgg tagttttcta tttttagctg
    26941 ctattttgac tatttgcttt atcaaggaag attttcaacc agtagccaag gaaaaggcta
    27001 ttccaacaaa ggaattattt acctcggtta aatatcccta tcttttgctc aatctctttt
    27061 taaccagttt tgtcatccaa ttttcagctc aatcgatcgg ccctattttg gctctttatg
    27121 tacgcgactt agggcagaca gagaatcttc tttttgtctc tggtttgatt gtgtccagta
    27181 tgggcttttc cagtatgatg agtgcaggag tcatgggcaa gctaggtgac aaggtgggca
    27241 atcatcgtct cttggttgtc gcccagtttt attcagtcat catctatctc ctctgtgcca
    27301 atgcctctag cccccttcaa ctaggactct atcgtttcct ctttggcttg ggaaccggtg
    27361 ccttgattcc cggggttaat gccctactca gcaaaatgac tcccaaagcc ggcatttcga
    27421 gggtctttgc cttcaatcag gtattctttt atctgggagg tgttgttggt cccatggcag
    27481 gttctgcagt agcaggtcaa tttggctacc atgctgtctt ttatgcgaca agcctttgtg
    27541 ttgcctttag ttgtctcttt aacctgattc aatttcgaac attattaaaa gtaaaggaaa
    27601 tctagtgcga gtaaaaatta atctcaaatg ctcctcttgt gacagtatca attacctaac
    27661 cagtaaaaat tcaaaaaccc atccagacaa gattgaggtt ttaaagtatt gtcccaagga
    27721 aagaaaagta accctacatc ttgaatctaa gtagatttta tggtaaaata ata
//
LOCUS       DAVGJL010000014        24398 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.014, whole genome shotgun sequence.
ACCESSION   DAVGJL010000014 DAVGJL010000000
VERSION     DAVGJL010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24398
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.014"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            71..1249
                     /locus_tag="VVE42_000519"
     CDS             71..1249
                     /locus_tag="VVE42_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEU3072768.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQ
                     SLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLL
                     EQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKV
                     RPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIA
                     CWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD
                     "
     gene            1473..2252
                     /gene="rpsB"
                     /locus_tag="VVE42_000520"
     CDS             1473..2252
                     /gene="rpsB"
                     /locus_tag="VVE42_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEU3072769.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAAEAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDAVAVEAEFAASETQADSIEEIVEVVEGDNA"
     gene            2330..3370
                     /locus_tag="VVE42_000521"
     CDS             2330..3370
                     /locus_tag="VVE42_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEU3072770.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVVEGGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKAQLTDDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
     gene            3616..3939
                     /gene="tnpB"
                     /locus_tag="VVE42_000522"
     CDS             3616..3939
                     /gene="tnpB"
                     /locus_tag="VVE42_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEU3072771.1"
                     /translation="MMIQLSDLGQVHIVCGKTDMRQGIDSLAYVVKTHFELDSFSGQV
                     FLFCGGRKDRFKVLYWDGQGFWLLYKRFENGKLTWLSTEKDVKALTSEQVDWLMKDFS
                     ITPQI"
     gene            complement(4080..4369)
                     /locus_tag="VVE42_000523"
                     /pseudo
     CDS             complement(4080..4369)
                     /locus_tag="VVE42_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4366..4764
                     /locus_tag="VVE42_000524"
     CDS             4366..4764
                     /locus_tag="VVE42_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU3072772.1"
                     /translation="MDAPIPKAPLAHSFGSASIIAHIIHQKFNLKVPNYRQEEDWAKM
                     GLPITRKEIANWHIKASQYYLEPLYNLLRERLLTQSLLHADETSYRVLESDSQLTYYW
                     TFLSGKAEKQGITLYHHVLIDLFISYFNPL"
     gene            complement(4961..5881)
                     /gene="cysK"
                     /locus_tag="VVE42_000525"
     CDS             complement(4961..5881)
                     /gene="cysK"
                     /locus_tag="VVE42_000525"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEU3072773.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKLGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESAILSGEKPGPH
                     KIQGISAGFIPDTLDTKAYDGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     VAKKLGTGKKVLTLAPDNGERYLSTALYEL"
     gene            complement(6166..6801)
                     /locus_tag="VVE42_000526"
     CDS             complement(6166..6801)
                     /locus_tag="VVE42_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEU3072774.1"
                     /translation="MEFRTIKEDGQVQEEIKKSRFICHAKRVYSEEEARDFITAIKKE
                     HYKATHNCSAFIIGERSEIKRTSDDGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYAGSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHDLMEL
                     DTNFTDQVDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLREVEVPVNLV"
     gene            6857..8155
                     /locus_tag="VVE42_000527"
     CDS             6857..8155
                     /locus_tag="VVE42_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU3072775.1"
                     /translation="MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     STILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLDLYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPML
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLAEILQEQFPNEKIGFVS
                     SVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVIEANHRLFTKSSLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL"
     gene            8152..8814
                     /locus_tag="VVE42_000528"
     CDS             8152..8814
                     /locus_tag="VVE42_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEU3072776.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQDCQLWCKEGVEVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            8894..9442
                     /gene="raiA"
                     /locus_tag="VVE42_000529"
     CDS             8894..9442
                     /gene="raiA"
                     /locus_tag="VVE42_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEU3072777.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNVVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
     gene            9578..11551
                     /locus_tag="VVE42_000530"
     CDS             9578..11551
                     /locus_tag="VVE42_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEU3072778.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTALLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDVSR
                     EQAITDELVTSRPVIGIVSVDNYDDLEDATSESDISQINSFVANFISEFSEKYMMFSR
                     RVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDEIG
                     KVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISDKI
                     RSVDQVFVVGHKNLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFIEK
                     EGVTKLLSVKDAMGMVTNRSLLILVDHSKTALTLSKEFYDLFTQTIVIDHHRRDQDFP
                     DNAVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGRKLGSDVLIAEAKDTKCY
                     DTVVISKAADAMLAMSGIEASFVLAKNTQGFISISARSRSKLNVQRIMEELGGGGHFN
                     LAAAQIKDVTLSEAGEKLTEIVLNEIKEKEKEE"
     gene            11548..12000
                     /gene="rplI"
                     /locus_tag="VVE42_000531"
     CDS             11548..12000
                     /gene="rplI"
                     /locus_tag="VVE42_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEU3072779.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            12044..13396
                     /gene="dnaB"
                     /locus_tag="VVE42_000532"
     CDS             12044..13396
                     /gene="dnaB"
                     /locus_tag="VVE42_000532"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEU3072780.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDRGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            13398..13664
                     /locus_tag="VVE42_000533"
     CDS             13398..13664
                     /locus_tag="VVE42_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3072781.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            13753..15099
                     /gene="cbpD"
                     /locus_tag="VVE42_000534"
     CDS             13753..15099
                     /gene="cbpD"
                     /locus_tag="VVE42_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HEU3072782.1"
                     /translation="MKILPFIARGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSRG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEIPAAYGNANEWG
                     HRARREGYRVDNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRI
                     IKANTMTGFIHFKDLDGGSVGNSQSSASTGGTHYFKTKSAIKTEPLASGTVIDYYYPG
                     EKVHYDQILEKDGYKWLSYTAYNGSYRYVQLEAVNKNPLGHSVLSSTGGTHYFKTKSA
                     IKIEPLVSATVIDYYYPGEKVHYDQILEKDGYKWLSYTAYNGSRRYIQLEGVTSSQNY
                     QNQSGNISSYGSNNSSTVGWKKINGSWYHFKSNGSKSTGWLKDGSSWYYLKLSGEMQT
                     GWLKENGSWYYLGSSGAMKTGWYQVSGKWYYSYSSGALAVNTTVDGYRVNSDGERV"
     regulatory      15182..15284
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            15355..15645
                     /locus_tag="VVE42_000535"
     CDS             15355..15645
                     /locus_tag="VVE42_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HEU3072783.1"
                     /translation="MKASIALQVLPLAQGIDRIAVIDQVIAYLQTQEVTMVVTPFETV
                     LEGEFDELMRILKEALEVAGQEADNVFANVKINVGEILSIDEKLEKYTETTH"
     gene            15608..16366
                     /locus_tag="VVE42_000536"
     CDS             15608..16366
                     /locus_tag="VVE42_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU3072784.1"
                     /translation="MRNLRSILRRHISLLGFLGVLSIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWFNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     NPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRT"
     gene            16404..17411
                     /locus_tag="VVE42_000537"
     CDS             16404..17411
                     /locus_tag="VVE42_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU3072785.1"
                     /translation="MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            17411..18139
                     /locus_tag="VVE42_000538"
     CDS             17411..18139
                     /locus_tag="VVE42_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3072786.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPKGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AEAISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLDK"
     gene            18258..18716
                     /locus_tag="VVE42_000539"
     CDS             18258..18716
                     /locus_tag="VVE42_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEU3072787.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRELLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLESVALDRVLGEEAPVVRANMLRQIIQEVDRKGK"
     gene            18718..21150
                     /locus_tag="VVE42_000540"
     CDS             18718..21150
                     /locus_tag="VVE42_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU3072788.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYEMDRLEEVALELTETDYSQDETFTELPFSRRLQVLFDEAEYVASVVHAKV
                     LGTEHVFYAILHDGNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALALGLAQRIASGDVPAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     TLRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKAKHVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLVTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLAE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGNTLKI
                     GVKAGQLKFDIA"
     gene            21792..22445
                     /gene="rr06"
                     /locus_tag="VVE42_000541"
     CDS             21792..22445
                     /gene="rr06"
                     /locus_tag="VVE42_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HEU3072789.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDLWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            22442..23773
                     /gene="hk06"
                     /locus_tag="VVE42_000542"
     CDS             22442..23773
                     /gene="hk06"
                     /locus_tag="VVE42_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HEU3072790.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILLLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGKQINGMYEHLLKVIHE
                     LESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIAKSI
                     NKIDQMSHLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIENQLT
                     DATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQELEH
                     LFDIFYHSQIVTDKDESSGLGLYIVNNILESYQMDYSFLPYEHGMEFKISL"
     gene            23856..24383
                     /locus_tag="VVE42_000543"
     CDS             23856..24383
                     /locus_tag="VVE42_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEU3072791.1"
                     /translation="MVLAIILVTFLIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     MAHIVFYLSCLVEAMVHKTIFDSMGMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKYVDHILFRTVKHPNYFLNILPELIGLTLLSHAYMTFVLVFPVYAVILYRRIAEEE
                     KLLHEVIIPNGSIKR"
BASE COUNT         7617 a         4113 c         5447 g         7221 t
ORIGIN      
        1 ggcgtaatat ttattaggcc tttttttggt atactagtat tgtctttaaa agaaggagta
       61 tctacgtaat atgaagaaaa aaatcttagc gtcactttta ttaagtacag taatggtttc
      121 tcaagtagct gttttaacaa ctgcgcatgc agaaacgact gatgacaaaa ttgctgctca
      181 agataataaa attagtaact taacagcaca acaacaagaa gcccaaaaac aagttgacca
      241 aattcaggag caggtatcag ctattcaagc tgagcagtct aacttgcaag ctgaaaatga
      301 tagattacaa gcagaatcta agaaactcga gggtgagatt acagaacttt ctaaaaacat
      361 tgtttctcgt aaccaatcgt tggaaaaaca agctcgtagt gctcaaacaa atggagccgt
      421 aactagctat atcaatacca ttgtaaactc aaaatcaatt acagaagcta tttcacgtgt
      481 tgctgcaatg agtgaaatcg tatctgcaaa caacaaaatg ttagaacaac aaaaggcaga
      541 taaaaaagct atttctgaaa aacaagtagc aaataatgat gctatcaata ctgtaattgc
      601 taatcaacaa aaattggctg atgatgctca agcattgact acgaaacagg cagaactaaa
      661 agctgctgaa ttaagtcttg ctgctgagaa agcgacagct gaaggggaaa aagcaagtct
      721 attagagcaa aaagcagcag ctgaggcaga ggctcgtgca gctgcggtag cagaagcagc
      781 ttataaagaa aaacgagcta gccaacaaca atcagtactt gcttcagcaa acactaactt
      841 aacagctcaa gtgcaagcag tatctgaatc tgcagcagca cctgtccgtg caaaagttcg
      901 tccaacatac agtacaaacg cttcaagtta tccaattgga gaatgtacat ggggagtaaa
      961 aacattggca ccttgggctg gagactactg gggtaatgga gcacagtggg ctacaagtgc
     1021 agcagcagca ggtttccgta caggttcaac acctcaagtt ggagcaattg catgttggaa
     1081 tgatggtgga tatggtcacg tagcggttgt tacagctgtt gaatcaacaa cacgtatcca
     1141 agtatcagaa tcaaattatg caggtaatcg tacaattgga aatcaccgtg gatggttcaa
     1201 tccaacaaca acttctgaag gttttgttac atatatttat gcagattaat ttacagaggg
     1261 actcgaatag agccctcttt tcaggtttta ccgtgacaat ccctattaaa aattatatca
     1321 aaatagcttg aaaatattgg aaaagtatgg tagaatgaaa attgtcgtgt gaacgataat
     1381 actcattctt gatgaattgt gaagcagttg cccttgggtc gttttgcgag ttgaagtcaa
     1441 gaagaggaaa aaaacaaaaa ggagaaatac tcatggcagt aatttcaatg aaacaacttc
     1501 ttgaggctgg tgtacacttt ggtcaccaaa ctcgtcgctg gaatcctaag atggctaagt
     1561 acatctttac tgaacgtaac ggaatccacg ttatcgactt gcaacaaact gtaaaatacg
     1621 ctgaccaagc atacgacttc atgcgtgatg cagcagctaa cgatgcagtt gtattgttcg
     1681 ttggtactaa gaaacaagca gctgaagcag ttgctgaaga agcagtacgt tcaggtcaat
     1741 acttcatcaa ccaccgttgg ttgggtggaa ctcttacaaa ctggggaaca atccaaaaac
     1801 gtatcgctcg tttgaaagaa attaaacgta tggaagaaga tggaactttc gaagttcttc
     1861 ctaagaaaga agttgcactt cttaacaaac aacgtgcgcg tcttgaaaaa ttcttgggcg
     1921 gtatcgaaga tatgcctcgt atcccagatg tgatgtacgt agttgaccca cataaagagc
     1981 aaatcgctgt taaagaagct aaaaaattgg gaatcccagt tgtagcgatg gttgacacca
     2041 atactgatcc agatgatatc gatgtaatca tcccagctaa cgatgacgct atccgtgctg
     2101 ttaaattgat cacagctaaa ttggctgacg ctattatcga aggacgtcaa ggtgaggatg
     2161 cagtagcagt tgaagcagaa tttgcagctt cagaaactca agcagattca attgaagaaa
     2221 tcgttgaagt tgtagaaggt gacaacgctt aatttataca aatagtaatt acctaggagg
     2281 gcggggctta gcccggctct cctattttca aaaatatagg agaattaaaa tggcagaaat
     2341 tacagctaaa cttgtaaaag agttgcgtga aaaatctggt gccggtgtta tggacgctaa
     2401 aaaagcgctt gtagaaacag acggtgacat cgaaaaagcg attgaattgc ttcgtgaaaa
     2461 aggtatggct aaggcagcta agaaagctga ccgtgttgct gcagaaggtt tgactggtgt
     2521 ttatgttaac ggtaatgttg cagcagttat tgaagtaaac gctgaaactg acttcgttgc
     2581 aaaaaacgct caattcgttg aattggtaaa tactacagct aaagtcattg ctgaaggaaa
     2641 acctgctaac aatgaagaag ctcttgcttt gataatgcct tcaggtgaaa ctcttgaagc
     2701 tgcatacgta tctgcaacag caactatcgg agagaaaatc tcattccgtc gctttgcatt
     2761 gattgaaaaa acagacgcac aacactttgg agcataccaa cataacggtg gacgtatcgg
     2821 tgttatttca gttgttgaag gtggagacga agcacttgct aaacaattgt caatgcacat
     2881 cgcagcgatg aaaccaacag ttctttctta caaagaattg gatgagcaat tcgttaaaga
     2941 tgagttggca caattgaatc atgttatcga ccaagacaac gaaagccgtg caatggttaa
     3001 taaaccagct cttccacact tgaagtatgg atcaaaagct caattaactg atgatgttat
     3061 tgctcaagct gaagctgaca tcaaagctga attggctgca gaaggcaaac cagaaaaaat
     3121 ctgggacaaa attatcccag gtaaaatgga tcgcttcatg cttgataata ctaaagttga
     3181 ccaagcttac acacttcttg cacaagttta catcatggat gacagcaaga cagttgaagc
     3241 ataccttgaa tcagttaacg cttcggtagt tgagtttgct cgctttgaag ttggtgaagg
     3301 tatcgagaaa gctgcaaacg acttcgaagc tgaagttgca gctacaatgg cagcagcctt
     3361 gaataactaa taatagaaag gaagcaattt gcttcctttt ttgtataggg cttagatttt
     3421 tcctgaaatt gtttttcccc cagtctcact cgattagaat aattaaaaca aaaagcccta
     3481 taataagggc taagtgcatt taggagacta tacttcaaat tcatagtaag cgcgtcataa
     3541 caaggtatct atcctaattc cccaacttcc agtatactag ataaaaaata attatcggag
     3601 gaaaggtatt gtactatgat gattcaacta agtgatttag gtcaagttca cattgtttgt
     3661 ggcaagacag atatgaggca gggaatagac tcattagcct atgtagttaa aacccacttt
     3721 gaattggatt ctttctccgg tcaagtcttt ctcttttgtg gtggacgtaa agaccgcttt
     3781 aaagtccttt actgggatgg tcaaggattt tggctactat ataaacgctt tgagaacgga
     3841 aaactgactt ggctaagtac agaaaaggat gtcaaagctc tcacatctga acaagtagac
     3901 tggcttatga aggacttttc tatcactccc caaatatagt ggtttgaaac tagaatagta
     3961 cacctctgct tctaaaacat tgttagaaat cgatttgact gtcctgttct tatttcattt
     4021 tactataaat tcatcagaaa gtcgtgattt ctattgaaat gaggactttc tttttatact
     4081 catctgtttt cacaaagcat tctagtccat ttccgattaa cgatggactt tatcacctcc
     4141 ttctccagtc cttgtataac atcttggagt tgattcatga catcttccaa aatttgaaag
     4201 gctttattct taaatccacg tttacgaatc tctttccaca cttgttcaat gggttcatct
     4261 ctggtgtgta tggaggaata aaggtaaaac caatattagt cggaatcttt aaggtacttg
     4321 atttatgcca tatagcattg tccataacga gtaaaaaata atcatctgga tgctcctatt
     4381 cctaaagccc ctttagcgca tagctttggc tcagcttcta ttatcgctca catcatccat
     4441 cagaagttta atctgaaggt acccaattat cgccaagaag aagattgggc taagatgggt
     4501 ttaccaatca cacgtaagga aattgctaat tggcatatca aggcgagtca atactatttg
     4561 gagccccttt ataacctctt gcgagagaga ctattgacgc agtccttact tcatgcggat
     4621 gaaacttctt atagggtgct agagagtgat agtcagctga cttactattg gacttttttg
     4681 tctgggaaag ctgagaaaca agggattacg ctttaccatc atgtcctaat cgatttgttc
     4741 atatcttatt tcaatccatt ataaatagtg agaaatattt atcctatctt ctagaatgtc
     4801 ttccaaacgt ggaaactctc gtaaacaaag aggttttaga ggcctattta ccatggacta
     4861 aagttgtaca agaaaagtgc aaataagaaa tctccagatt aggaactatc cgtgagttct
     4921 ctagtctgga gatttttcaa tatacttcgt tattggatgg ttacaattca taaagtgctg
     4981 tagagagata acgttcaccg ttatctggtg ctagggtaag gactttttta cctgtaccta
     5041 attttttggc aacctcgatg gctccgtaga tagctgcagc tgaggaaatc cctacaagga
     5101 agccttcttt tccaccaatt tcacgtccga gtgcaagagc gtcatctgat gttacacgaa
     5161 cgataccatc ataggcttta gtatcaagtg tatcaggaat aaatccagct gagatacctt
     5221 gaattttgtg aggaccaggt ttttcaccag atagaatagc agattcatct gcttctactg
     5281 caaaaacttg aatgttagaa ttttctgatt tgagtgcatg agaaacacca gaaatcgttc
     5341 caccggtacc tactccagca acaaaggcat ctaatccatc tttaccgaaa gcagctagta
     5401 tctcagctcc tgttgttctt tcgtgtactt ctggattagc tggattgtca aattgaagag
     5461 gaaggaaacc atcacgttca gcagcgattt cttgagcctt agcaatagca cctttcattc
     5521 cctcgctacc aggagttagg acgagttcag caccataagc ttggataatt ttacgtcgtt
     5581 ctacactcat agtttcaggc ataacgatga cgactttata ccctttagca gcacctaccc
     5641 atgaaagtcc aataccggtg tttccacttg ttgcttcaac aatagtagaa ccaagtttca
     5701 gaataccatc ttgttcagct ttttcaatca tgctaagggc aatacggtct tttacagatg
     5761 aaccaggatt aaatgcttca agctttatat agacgtctgc agcaccttct ggcacgatgt
     5821 tgttaagttt aacaatcggt gtttgaccga ttaattcagt aatgttgtta taaatagaca
     5881 tatgaatacc tctttgtata agatatcttt agtataacgc tatccatacc atttgtaaaa
     5941 tatagaaatc ctatcgaagc gataggattt ctatatatca tagatcaaag tcattaattt
     6001 tcaagtgatt gcgttcagtt aattctagta aataggtgtg aaggaaaacg atatcaggtt
     6061 tcgtttgaat tgcttcattt tctagtatat tttggtacgc atctttttat tttgctcgct
     6121 aaagtagttg tgagaatgtc aacgaaacgc tgtattattc attgtttaca ctaagtttac
     6181 aggaacttca acctctcgta aaccttggtc agttaaagtg acttttccat taaaaaactc
     6241 cacaagtgca gctttaatag tttctttttc ttctttatca acataaatca tcgtatcgac
     6301 ttgatctgta aagtttgtat ccagctccat gagatcatgt tctttaagga agttactgta
     6361 ctcttggtac tgagcataag acatttgaat agcaatgcca gcctgttctt ttatttcaat
     6421 aataccaatt tctttgacag ctaaggcgac actgccggcg taagcacgaa ttagtcctcc
     6481 agcgcctagt ttaataccac caaagtagcg tgtcacgacc acacagacat tggtgagatt
     6541 gtgattttct agtaccccaa gcatgggaac accagcagta ccactaggct caccatcatc
     6601 acttgtacgt ttaatttcac tacgttctcc aataataaag gcagagcagt tatgtgtcgc
     6661 tttgtagtgt tcttttttga tggcagtaat gaagtcacga gcctcttctt cgctataaac
     6721 acgcttggca tggcagataa agcgagattt tttgatttct tcttggactt gaccgtcctc
     6781 tttaattgtt ctaaattcca tgatttaatt gtactaaaaa atcatctaaa gtgctagttt
     6841 ttacgaataa agaagtatga aagtaaattt agattatctc ggtcgtttat ttactgagaa
     6901 tgaattaaca gaagaagaac gtcagttggc ggagaaactt ccagcaatga gaaaggagaa
     6961 ggggaaactt ttctgtcaac gttgtaatag tactattcta gaagaatggt atttgcccat
     7021 cggtgcttac tattgtcgag agtgcttgct gatgaagcga gtcagaagtg atcaaacttt
     7081 atactatttt ccgcaggagg attttccaaa gcaagatgtt ctcaaatggc gcggccaatt
     7141 aactcctttt caagagaagg tgtcagaggg attgcttcaa gcagtagaca agcaaaagcc
     7201 aaccttagtt catgcggtaa caggagctgg aaagacagaa atgatttatc aagtagtggc
     7261 taaagtgatc aatgcgggtg gtgcagtgtg tttggctagt cctcgcatag atgtttgttt
     7321 ggatctgtac aagcgcctgc aacaggattt ttcttgcggg atagctttgc tacatggaga
     7381 atcggaacct tattttcgaa caccactagt tgttgcaaca acccatcagt tattgaagtt
     7441 ttatcaagct tttgatttgc tgatagtgga tgaagtagat gcttttcctt atgttgataa
     7501 tcccatgctt taccacgctg tcaagaatag tgtaaaggag aatggattga gaatcttttt
     7561 aacagcgact tcgaccaatg agttagataa aaaggttcgt ttaggagaac taaaaagact
     7621 gaatttaccg agacggtttc atggaaatcc gttgattatt ccaaaaccaa tttggttatc
     7681 ggattttaat cgctacttag acaagaatcg tttgtcacca aagttaaagt cctatattga
     7741 gaagcagaga aagacagctt atccgttact catttttgct tcagaaatta agaaagggga
     7801 gcagttagca gaaatcttac aggagcaatt tccaaatgag aaaattggct ttgtatcttc
     7861 tgtaacagag gatcgattag agcaagtaca agcttttcga gatggagaac tgacaatact
     7921 tatcagtacg acaatcttgg agcgtggagt taccttccct tgtgtggatg ttttcgtaat
     7981 agaggccaat catcgtttgt ttaccaagtc tagtttgatt cagattggtg gacgagttgg
     8041 acgaagcatg gatagaccga caggagattt gcttttcttc catgatgggt taaatgcttc
     8101 aatcaagaag gcgattaagg aaattcagat gatgaataag gaggctggtc tatgaagtgc
     8161 ttgttatgtg ggcagactat gaagactgtt ttaactttta gtagtctctt acttctgagg
     8221 aatgatgact cttgtctttg ttcagactgt gattctactt ttgaaagaat tggggaagag
     8281 aactgtccaa attgtatgaa aacagagttg tcgacaaagt gtcaagattg tcaactttgg
     8341 tgtaaagaag gagttgaagt cagtcataga gcgattttta cttacaatca agctatgaag
     8401 gattttttca gtcggtataa gtttgatgga gacttcctgt taagaaaagt tttcgcttca
     8461 tttttaagtg aggagttgaa aaagtacaaa gagtatcaat ttgttgtaat tcccctaagt
     8521 cctgatagat atgctaacag aggatttaat caggttgagg gcttggtaga ggcagcaggc
     8581 tttgagtatc tagatttatt agagaaaaga gaagagagag ccagttcttc taaaaatcgt
     8641 tcagagcgct tggggacaga acttcctttc tttattaaaa gtggagtcac tattcctaaa
     8701 aaaatcttac ttatagatga tatctatact acaggagcaa ctataaatcg tgttaagaaa
     8761 ctgttggaag aagctggtgc taaggatgta aaaacatttt cccttgtaag atgaggaaaa
     8821 aatttgcaaa aatagaatga aagcgttata ataaaatcat aaaaacaaaa atgtttagaa
     8881 agaaggtact catatgatta aatatagtat ccgtggtgaa aacctagaag taacagaagc
     8941 aattcgtgat tatgtagttt ctaaactcga aaagatcgaa aagtacttcc aaccagaaca
     9001 agagttggat gcccgaatta acttaaaagt ttatcgtgaa aaaacggcta aagtggaagt
     9061 aacgattccg cttggatcta ttactctccg cgcagaagat gtatctcaag atatgtatgg
     9121 ttcaattgac cttgtaactg ataaaattga acgtcagatt cgtaaaaata aaacaaaaat
     9181 cgagcgtaaa aataaaaata aggtagcaac tggtcaatta tttacagatg ctttggtgga
     9241 agattcaaat gttgtccagt ctaaagttgt tcgttcaaaa caaattgatt taaaaccaat
     9301 ggatttggaa gaagcaattc tacaaatgga tttattgggg catgatttct ttatctatgt
     9361 ggatgttgaa gatcagacaa ccaatgtgat ttatcgtcgt gaggatggcg aaattggttt
     9421 gttagaggtt aaagaatctt aatttcaata tgtgtaaagg taggtttact gaattgtaaa
     9481 ctaccttttt cttataattg atagaattac tgattacact tttaaaaagt cgctttttga
     9541 ggggtattat ggtataatgg gtgccaagag gttttgaatg aaaaaatttt atgtaagtcc
     9601 aatttttcct attctagtag gattgattgc gtttggagtc ttatccactt tcattatttt
     9661 tgttaataat aatctgttga cggttttaat tttgtttctt tttgtaggag gctatgtttt
     9721 tttatttaag aaactgagag tgcattatac aaggagtgat gtagaacaga tacagtatgt
     9781 aaaccaccaa gcggaagaaa gtttgacagc tctattggaa cagatgcctg taggtgttat
     9841 gaaattgaat ttatcttctg gagaggttga gtggtttaat ccctatgctg aattgatttt
     9901 gaccaaggaa gatggtgatt ttgatttaga agctgttcaa acgattatca aggcttcagt
     9961 aggaaatccg tctacttatg ccaagcttgg tgagaagcgt tatgctgttc atatggatgc
    10021 ttcttccggt gttttgtatt ttgtagatgt atccagggaa caagccataa cagatgaatt
    10081 ggtaacaagt agaccagtga ttgggattgt ctctgtggat aattatgatg atttggagga
    10141 tgcaacttct gagtcagata ttagtcaaat caatagtttt gtagctaatt ttatatcaga
    10201 gttttcagaa aaatacatga tgttttctcg tcgggtaagt atggatcgat tttatctatt
    10261 tactgactac acggtgcttg agggcttgat gaatgataaa ttttctgtta ttgatgcttt
    10321 cagagaagag tcgaaacaga gacagttgcc cttgacctta agtatgggat tttcttatgg
    10381 cgatggaaat catgatgaga tagggaaagt tgctttgctc aatttgaact tggctgaagt
    10441 acgtggtggc gaccaggtgg ttgttaagga aaacgacgaa acgaaaaatc cagtttattt
    10501 tggtggtggg tctgctgctt caatcaagcg tacacggact cgtacgcgcg ctatgatgac
    10561 agctatttca gataagattc ggagtgtaga tcaggttttt gtagtcggtc acaaaaattt
    10621 agacatggat gctttgggct ctgctgtagg tatgcagttg ttcgccagca atgtgattga
    10681 aaatagctat gctctttatg atgaagaaca aatgtctcca gatattgaac gagctgtttc
    10741 attcatagaa aaagaaggag ttacgaagtt gttgtctgtt aaggatgcaa tggggatggt
    10801 gaccaatcgt tctttgttga ttcttgtaga ccattcaaag acagccttaa cattatcaaa
    10861 agaattttat gatttattta cccaaaccat tgttattgac caccatagaa gggatcagga
    10921 ttttccagat aatgcagtca ttacttatat cgaaagtggt gcaagtagtg ccagtgagtt
    10981 ggtaacggaa ttgattcagt tccagaattc taagaaaaat cgtttgagtc gtatgcaagc
    11041 aagtgtcttg atggctggta tgatgttgga tactaaaaat ttcacctcgc gagtaactag
    11101 tcggacattt gatgttgcta gctatctcag aacgcgcgga agtgatagta ttgctatcca
    11161 ggaaatcgct gcgacagatt ttgaagaata tcgtgaggtc aatgaactta ttttacaggg
    11221 gcgtaaatta ggttcagatg tactaatagc agaggctaag gacacgaaat gctatgatac
    11281 agttgttatt agtaaggcag cagatgccat gttagccatg tcaggtattg aagcgagttt
    11341 tgttcttgcg aagaatacac aaggatttat ctctatctca gctcgaagtc gtagtaaact
    11401 gaatgtacaa cggattatgg aagagttagg cggtggaggc cactttaatt tggcagcagc
    11461 tcaaattaaa gatgtaacct tgtcagaagc aggtgaaaaa ctgacagaaa ttgtattaaa
    11521 tgaaataaag gaaaaggaga aagaagaatg aaagtaatct ttttagcaga tgttaaagga
    11581 aaaggtaaaa aaggcgaaat taaggaagta ccaacagggt atgcgcaaaa ctttcttatc
    11641 aaaaagaatc tagccaaaga agcgactgct caagctgtag gtgaacttcg tggtaaacaa
    11701 aaatcggaag aaaaagctca cgctgagatg attgcagaag gaaaagcaat taaagcacaa
    11761 cttgaagcag aagaaactgt tgtagaattt gttgaaaaag ttggtccaga tggtcgtacc
    11821 tttggttcta ttaccaataa gaagattgca gaagaattgc aaaagcaatt tggaattaag
    11881 attgataaac gtcatattca agtacaagct ccgattcgag cggttggttt gattgatgtg
    11941 ccagtgaaaa tctatcaaga tatcacaagt gtaatcaatc ttcgtgtgaa agaaggataa
    12001 gtttacacct tcttgacaag attgtaaaag gaagggaagt ctgatggcag aagtagaaga
    12061 gttacgagta caacctcaag atatcttagc tgagcaatcc gttttagggg ctatctttat
    12121 tgatgagagt aaacttgttt ttgtgcgaga atacattgag tctcgggact tttttaagta
    12181 tgcccatcgt ttgattttcc aagccatggt cgatttatcc gatcgtggtg atgccataga
    12241 tgcaacaacg gttcgtacta tccttgataa tcaaggtgat ttacagaata ttggtggctt
    12301 gtcttacttg gttgagattg ttaattctgt gccaacttct gctaatgcgg agtattatgc
    12361 taagattgtt gcagaaaaag caatgctacg tcgtttaatt gccaagttga cagagtctgt
    12421 caaccaagct tacgaagcgt cacaaccagc tgatgaaatt attgctcagg cagaaaaagg
    12481 gttgattgat gtcagtgaaa atgcaaatcg aagcgggttt aagaacattc gagatgtgtt
    12541 gaatctcaac tttggaaatc tggaagctcg ctcgcaacaa acgaccgata ttacaggtat
    12601 tgcgacaggt tatcgtgatt tggatcatat gacaacagga cttcatgagg aggagttgat
    12661 tatcttagca gctcgtccag cagttggtaa gacagcattt gccttgaata tcgctcagaa
    12721 cattgggact aagttggaca aaacggttgc tattttttca ctcgaaatgg gtgcggaaag
    12781 cttggtagat cgtatgttag ctgcagaagg cttagtggag tcacattcta tccgtacagg
    12841 gcaattgaca gatgaggagt ggcaaaaata tactattgct cagggtaatc tagctaacgc
    12901 cagtatctat atcgatgata cgccaggtat tcggattaca gagattcgtt ctcgttctcg
    12961 taaattggct caagaaactg gaaatcttgg tttgattttg atagactatt tgcaacttat
    13021 cacgggaact ggtcgagaaa atcgtcaaca agaagtttct gaaatttctc gtcagttgaa
    13081 aatactagcc aaggaattga aggttccagt aatcgctctg agtcagcttt ctcgtggtgt
    13141 agaacaacgt caggacaaga gaccagtctt gtctgatatt cgtgaatctg ggtctattga
    13201 gcaggacgct gatatcgtag cttttctcta tcgcgatgac tactatgaac gtggtggtga
    13261 agaagaggag ggtatcccaa ataataaggt ggaagttatt atcgagaaaa accgtagtgg
    13321 agctcgtgga acagtggaat tgattttcca aaaagaatac aataaatttt caagtatctc
    13381 aaagagggag gcataagatg tcagatgcat ttacagatgt agccaagatg aaaaaaatca
    13441 aagaagaaat caaggcacat gagggacaag tcgtagaaat gactttggag aatggtcgta
    13501 agcgccaaaa aaatagattg ggtaagctaa ttgaagttta tccatctcta tttattgtgg
    13561 agtttgggga tgtggaagga gataaacaag ttaatgttta cgttgaatcc tttacttact
    13621 cagatattct tacagaaaag aatttgattc attatcttga ctaaagtgag aaattttctc
    13681 actttttctt ttttctccga ataatttagg tgaaggcaat catcgcttta tattattttt
    13741 caaggaggaa gaatgaaaat tttaccgttt atagcaagag gaacaagtta ttacttgaag
    13801 atgtcagtta aaaagcttgt tcctttttta gtagtaggat tgatgctagc agctggtgat
    13861 agtgtctatg cctattccag aggaaatgga tcgattgcgc gtggggatga ttatcctgct
    13921 tattataaaa atgggagcca ggagattgat cagtggcgca tgtattctcg tcagtgtact
    13981 tcttttgtag cctttcgttt gagtaatgtc aatggttttg aaattccggc agcttatgga
    14041 aatgcgaatg aatggggaca tcgtgctcgt cgggaaggtt atcgtgtaga taatacaccg
    14101 acgattggtt ccattacttg gtctactgca ggaacttatg gtcatgttgc ctgggtgtca
    14161 aatgtaatgg gagatcagat tgagattgag gaatataact atggttatac agaatcctat
    14221 aataaacgaa ttataaaagc aaacacgatg acaggattta ttcattttaa agatttggat
    14281 ggcggcagtg ttgggaatag tcaatcctca gcttcaacag gcggaactca ttattttaag
    14341 accaagtctg ctattaaaac tgaaccccta gctagcggaa ctgtgattga ttactattat
    14401 cctggggaga aggttcatta tgatcagata cttgaaaaag acggctataa gtggttgagt
    14461 tatactgcct ataatggaag ctatcgttat gttcaattgg aggctgtgaa taaaaatcct
    14521 ctaggtcatt ctgttctttc ttcaacagga ggaactcatt attttaagac caagtctgct
    14581 attaaaattg aacccctagt tagtgcaact gtgattgatt actattatcc tggagagaag
    14641 gttcattatg atcagatact tgaaaaagac ggctacaagt ggttgagtta tacggcttat
    14701 aacggaagtc gtcgctatat acagctagag ggagtgactt cttcacaaaa ttatcagaat
    14761 caatcaggaa atatctctag ctatggatcc aataatagtt caactgtcgg ttggaagaaa
    14821 ataaatggta gttggtatca tttcaaatca aatggttcta aatcaacagg atggctgaaa
    14881 gacggttcta gctggtatta tttgaaatta tctggtgaaa tgcagacagg atggttaaag
    14941 gagaatggct cgtggtatta tctgggtagt tcaggggcaa tgaaaacagg ctggtaccag
    15001 gtctctggta agtggtatta ttcttactct tcaggcgcct tagctgttaa tacgacggtg
    15061 gatggctaca gagtaaacag tgatggagaa cgagtataga aaattggagt aggagaaatt
    15121 tcctgctctt ttctgtaagc agtttccttg acatttttct tattttgcgc tatcatatat
    15181 acatataata tatgggagtc tgtgtacagt ctgagaggaa gtgttaaact tcgaccgcac
    15241 ctgatctggg taatgccagc ggagggaacg atacttagtc taattttgca ccttttccat
    15301 gtatggtaaa ggtttttctt tttttaaaaa ggaaaacgag aagaggaggt tcttatgaaa
    15361 gcaagcattg ccttgcaagt tttacccctg gcacagggga ttgatcggat agctgttatt
    15421 gatcaggtca ttgcttatct gcaaactcaa gaagtgacga tggtagtgac accatttgaa
    15481 acggtcttgg aaggggagtt tgatgagctt atgcgcattc taaaagaagc gctggaagtg
    15541 gcagggcagg aggcagacaa tgtctttgcc aatgtcaaaa taaatgtagg agagatttta
    15601 agtattgatg agaaacttga gaagtatact gagacgacac attagtctat tgggctttct
    15661 cggagtcttg tcaatctggc agttagcagg ttttcttaaa cttctcccca agtttatcct
    15721 gccgacacct cttgaaattc tccagccctt tgttcgtgac agagaatttc tctggcacca
    15781 tagctgggcg accttgagag tggctttact ggggctgatt ttgggagttt tgattgcctg
    15841 tcttatggct gtgctcatgg atagtttgac ttggttcaat gacctgattt accctatgat
    15901 ggtggtcatt cagaccattc cgaccattgc catagctcct atcctggtct tgtggctagg
    15961 ttatgggatt ttgcccaaga ttgtcttgat tatcttaaca acaacctttc ccatcatcgt
    16021 tagtattttg gacggtttta ggcattgtga caaggatatg ctgaccttgt ttagtctgat
    16081 gcgagcaaac ccttggcaaa tcctgtggca ttttaaaatc ccggttagcc tgccttactt
    16141 ttatgcaggt ctgagggtca gtgtctccta cgcctttatc acaactgtgg tatctgagtg
    16201 gttgggaggt tttgaaggtc ttggtgttta tatgattcag tctaaaaaac tgtttcagta
    16261 tgataccatg tttgccatta ttattctggt gtcgattatc agtcttttgg gtatgaagct
    16321 ggtcgatatt agtgaaaaat atgttattaa atggaaacgt acttaaaata gagcgttttt
    16381 gaaaaagaaa agaggaaatc aaaatgaaga aaacatggaa agtgttttta acgcttgtaa
    16441 cagctcttgt agctgttgtg cttgtggcct gtggtcaagg aactgcttct aaagacaaca
    16501 aagaggcaga acttaagaag gttgacttta tcctagactg gacaccaaat accaaccaca
    16561 cagggcttta tgttgccaag gaaaaaggtt atttcaaaga agctggagtg gatgttgatt
    16621 tgaaattgcc accagaagaa agttcttctg acttggttat caacggaaag gcaccatttg
    16681 cagtgtattt ccaagactac atggctaaga aattggaaaa aggagcagga atcactgccg
    16741 ttgcagctat tgttgaacac aatacatcag gaatcatctc tcgtaaatct gataatgtaa
    16801 gcagtccaaa agacttggtt ggtaagaaat atgggacatg gaatgaccca actgaacttg
    16861 ctatgttgaa aaccttggta gaatctcaag gtggagactt tgagaaggtt gaaaaagtac
    16921 caaataacga ctcaaactca atcacaccga ttgccaatgg cgtctttgat actgcttgga
    16981 tttactacgg ttgggatggt atccttgcta aatctcaagg tgtagatgct aacttcatgt
    17041 acttgaaaga ctatgtcaag gagtttgact actattcacc agttatcatc gcaaacaacg
    17101 actatctgaa agataacaaa gaagaagctc gcaaagtcat ccaagccatc aaaaaaggct
    17161 accaatatgc catggaacat ccagaagaag ctgcagatat tctcatcaag aatgcacctg
    17221 aactcaagga aaaacgtgac tttgtcatcg aatctcaaaa atacttgtca aaagaatacg
    17281 caagcgacaa ggaaaaatgg ggtcaatttg acgcagctcg ctggaatgct ttctacaaat
    17341 gggataaaga aaatggtatc cttaaagaag acttgacaga caaaggcttc accaacgaat
    17401 ttgtgaaata atgacagaaa ttagactaga gcacgtcagt tatgcctatg gtcaggagag
    17461 gattttagag gatatcaacc tacaggtgac ttcaggcgaa gtggtttcca tcctaggccc
    17521 aagtggtgtt ggaaagacca ccctctttaa tctaatcgct gggattttag aagttcagtc
    17581 agggagaatt gtccttgatg gtgaagaaaa tcccaagggg cgcgtgagtt atatgttgca
    17641 aaaggatctg ctcttggagc acaagacggt gcttggaaat atcattctgc ccctcttgat
    17701 tcaaaaggtg gataaggcag aagctatttc ccgagcggat aaaattcttg cgaccttcca
    17761 gctgacagct gtaagagaca agtatcctca tgaacttagc ggtgggatgc gccagcgtgt
    17821 agccttactc cggacctacc tttttgggca caagctcttt ctcttagatg aggcctttag
    17881 cgccttggat gagatgacaa agatggaact ccacgcttgg tatcttgaga ttcacaagca
    17941 gttgcagcta acaaccctga tcatcacgca tagtattgag gaggccctca atctcagcga
    18001 ccgcatctat atcttgaaaa atcgccctgg gcagattgtt tcagaaatta aactagattg
    18061 gtctgaagat gaggacaagg aagtccaaaa gattgcctac aaacgtcaaa ttttggcgga
    18121 attaggctta gataagtaga aaaataggga gttggtgaag attatccttt accagcgccc
    18181 tttttctttt aaaaatgaga aaatttcggt ataatagtca aacaaggtca aggtttaaag
    18241 agagaggtgg gtttgttatg agatttaaaa atacatcgga tcatattgag gcctacatca
    18301 aggcgatttt agatcaatct ggtatcgtgg agttgcaacg gagtcagttg gcagatacct
    18361 ttcaggttgt tcctagtcag attaactacg tgatcaagac acgctttacg gaaagtagag
    18421 gctacttggt tgaaagtaag cgtggtggcg gaggctacat tcgtatagga cggattgagt
    18481 tttctagtca tcatgaaatg ctccgggagc tgctttactc gattggtgag cgagtcagtc
    18541 aagaaattta tgaggatatt ctccagcttt tggttgagca ggaattgatg accaagcagg
    18601 agatgaattt gctagaatca gtagctttgg atcgcgtttt aggagaagaa gctccagttg
    18661 ttcgagcaaa catgctacgt cagatcatac aagaggtaga tagaaaaggg aagtaagatg
    18721 aactattcaa aagcattgaa tgaatgtatc gaaagtgcct acatggttgc tggacatttt
    18781 ggagctcgtt atctagagtc ttggcacttg ttgattgcca tgtctaatca cagttatagt
    18841 gtagcagggg caactttaaa tgattatccg tatgagatgg accgtttaga agaggtggct
    18901 ttggaactga ctgaaacgga ctatagccag gatgaaacct ttacggaatt gccgttctcc
    18961 cgtcgtttgc aggttctttt tgacgaagca gagtatgtag cgtcagtggt ccatgctaag
    19021 gtgctaggga cagagcacgt cttctatgcg attttgcatg atggcaatgc cttggcaact
    19081 cgtatcttgg agagggctgg tttttcttat gaagacaaga aagatcaggt caagattgct
    19141 gcccttcgtc gaaatttaga agaacgggca ggctggactc gtgaagacct caaggcttta
    19201 cgccaacgcc atcgtacagt agctgacaag caaaattcta tggccaatat gatgggcatg
    19261 ccgcagactc ctagtggtgg tcttgaggac tatacgcatg atttgacaga gcaagcgcgt
    19321 tctggcaagt tagaaccagt catcggtcgg gacaaggaaa tctcacgtat gattcaaatt
    19381 ttgagccgta agactaagaa caaccctgtc ttggttgggg atgctggtgt cgggaaaaca
    19441 gctctggccc ttggtcttgc ccagcgtatt gctagtggtg acgtgcctgc ggaaatggct
    19501 aagatgcgcg tgttagaact tgatttgatg aatgtcgttg cagggacacg cttccgtggt
    19561 gactttgaag aacgcatgaa taatatcatc aaggatattg aagaagatgg ccaagtcatc
    19621 ctctttatcg atgaactcca caccatcatg ggttctggta gcgggattga ttcgactctg
    19681 gatgcggcca atatcttgaa accagccttg gcgcgtggaa ctttgagaac ggttggtgcc
    19741 accactcagg aagaatatca aaaacatatc gaaaaagatg cggctctttc tcgtcgtttc
    19801 gctaaagtga cgattgaaga accaagtgtg gcagatagta tgactatttt acaaggtttg
    19861 aaggcgactt atgagaaaca tcaccgtgta caaatcacag atgaagcggt tgaaacagcg
    19921 gttaagatgg ctcatcgtta tttaaccagt cgtcacttgc cagactctgc tatcgacctc
    19981 ttggacgaag cagcagcaac agtgcaaaat aaggcaaagc atgtaaaagc agacgattca
    20041 gatttgagtc cagctgacaa ggccctgatg gatggcaagt ggaaacaggc agcccagcta
    20101 atcgcaaaag aagaggaagt acctgtctac aaagacttgg tgacagaatc tgatattttg
    20161 accaccttga gtcgcttgtc aggaatccca gttcaaaaac tgactcaaac ggatgctaag
    20221 aagtatttaa atcttgaagc agaactccat aaacgtgtca tcggtcaaga tcaagctgtt
    20281 tcaagtatta gtcgtgccat tcgccgtaat cagtcaggaa ttcgcagtca caagcgtccg
    20341 atcggttcct ttatgttcct aggacccaca ggtgtcggta agaccgagtt ggccaaggct
    20401 ttggcagaag ttctctttga tgacgaatca gcccttatcc gctttgatat gagtgagtat
    20461 atggagaaat tcgcagccag ccgtctaaat ggagctcctc caggctatgt gggttacgaa
    20521 gaaggtgggg agctgacaga gaaggttcgc aataaaccct attccgttct cctctttgat
    20581 gaggtagaga aggcccaccc agatatcttt aatgttctct tgcaggttct ggatgacggt
    20641 gtcttgacag atagcaaggg acgcaaggtc gatttttcaa ataccattat cattatgaca
    20701 tcgaatctag gtgcgactgc ccttcgtgat gataagactg ttggttttgg ggctaaggat
    20761 attcgttttg accaggaaaa tatggaaaaa cgcatgtttg aagaactgaa aaaagcttat
    20821 agaccggaat tcatcaaccg cattgatgag aaggtggtct tccatagcct atctagtgac
    20881 catatgcagg aagtggtgaa gattatggtc aagcctttag tggcaagttt ggctgaaaaa
    20941 ggcattgact tgaaattaca agcttcagct ctgaaattgt tagcaaatca aggatatgac
    21001 ccagagatgg gagctcgccc acttcgcaga accctgcaaa cagaagtgga ggacaagttg
    21061 gcagaacttc ttcttaaggg agatttagtg gcaggcaaca cacttaagat tggtgtcaaa
    21121 gcaggccagt taaaatttga tattgcataa aagaataaaa gtatcagcat ctgaccataa
    21181 gtcacagtgg agtgaaactc aatgaaaatc aaagagcaaa ctaggcagct agctgcaggt
    21241 tgctcaaaac actgttttga ggttgcagat agagctgacg tggtttgaag agattttcga
    21301 agagtatgaa actaaaacct atagcttcta aacgatccgt ggttttcacc attcaacaca
    21361 aaattcatat gtttattacc ctccgtcgta tttgtcttag agcgtgtgta gtagaaaaag
    21421 agcagtctta tctgaaattt ttattctttc aaaagagacc tgtttctttt ttgcatgtca
    21481 aatccgttct agctggtatt tgaaaaatca aactaatatt caatgaaaat caaagaacaa
    21541 actaggaagc tagccgcagg ttgctcaaaa cactgttttg aggttgtaga tagagctgac
    21601 gtggtttgaa gagattttcg aagagtataa gctgcaagat gaatgatttt cttgtattga
    21661 cgttgttgtt gacaaaaagt agcggataaa tgaaatccat tccattatca tagatgatag
    21721 gctggtagga aattttcaaa tagcatacag gaaatagatg tatggagttc tggtagtaga
    21781 aagggagaga gatgaacatt ttagttgcag atgacgagga aatgattaga gaaggaattg
    21841 cagcatttct gacagaagag ggttatcatg tcattatggc taaggatgga caagaggtct
    21901 tggaaaaatt tcaagatctc cctatccatc tcatggtact ggatttaatg atgcctagga
    21961 agagtggttt tgaagtgtta aaagaaatca atcaaaagca cgatattcct gtcatcgtct
    22021 tgagtgctct gggagatgaa actactcagt cacaggtatt tgatctctat gctgatgatc
    22081 atgtgacaaa acctttttct ttggtactgc ttgtcaagcg tattaaggcg cttatcagac
    22141 gttactacgt catagaggat ctttggcgat atcaggatgt aacagtggat tttacctctt
    22201 acaaagcaca ttataaaaat gaagaaattg atctcaaacc aaaggaatta ctggtactaa
    22261 agtgtttgat tcagcataaa aatcaagttt taagtagaga gcagatattg gaagaaattt
    22321 caaaagatgt agctgattta ccttgtgata gggtcgttga tgtctatatt cgtactcttc
    22381 gcaaaaaatt agctttagat tgtatcgtga ctgtgaaaaa tgttgggtat aagattagct
    22441 tatgataaaa aatcctaaat tattaaccaa gtctttttta agaagttttg caattctagg
    22501 tggtgttggt ctagtcattc atatagctat ttatttgacc tttccttttt attatattca
    22561 actggagggg gaaaagttta atgagagcgc aagagtgttt acggagtatt taaagactaa
    22621 gacatctgat gaaattccaa gcttactcca gtcttattca aagtccttga ccatatctgc
    22681 tcaccttaaa agagatattg tagataagcg gctccctctt gtgcatgact tggatattaa
    22741 agatggaaag ctatcaaatt atatcgtgat gttagatatg tctgttagta cagcagatgg
    22801 taaacaggta accgtgcaat ttgttcacgg ggtagatgtc tacaaagaag caaagaatat
    22861 tttgcttttg tatctcccat atacattttt ggttacaatt gctttttcct ttgttttttc
    22921 ttatttttat actaaacgct tgctcaatcc tcttttttac atttcagaag tgactagtaa
    22981 aatgcaagat ttggatgaca atattcgttt tgatgaaagt aggaaagatg aagttggtga
    23041 agttggaaaa cagattaatg gtatgtatga gcacttgttg aaggttattc atgagttgga
    23101 aagtcgtaat gagcaaattg taaaattgca aaatcaaaag gtttcctttg tccgcggagc
    23161 atcacatgag ttgaaaaccc ctttagccag tcttagaatt atcctagaga atatgcagca
    23221 taatattgga gattacaaag atcatccaaa atatattgca aagagtataa ataagattga
    23281 ccagatgagc cacttattgg aagaagtact ggagtcttct aaattccaag agtggacaga
    23341 gtgtcgtgag accttgactg ttaagccagt tttagtagat attttatcac gttatcaaga
    23401 attagctcat tcaataggtg ttacaattga aaatcaattg acagatgcta ccagggtcgt
    23461 catgagtctt agggcattgg ataaggtttt gacaaacctg attagtaatg caattaaata
    23521 ttcagataaa aatgggcgcg taatcatatc cgagcaagat ggctatctct ctatcaaaaa
    23581 tacatgtgcg cctctaagtg accaagaact agaacattta tttgatatat tctatcattc
    23641 tcaaatcgtg acagataagg atgaaagttc cggtttgggt ctttacattg tgaataatat
    23701 tttagaaagc tatcaaatgg attatagttt tctcccttat gaacacggta tggaatttaa
    23761 gattagcttg tagacagatt agttttttat taaagttcat atagggttaa cataagtgtg
    23821 ttattctttg tgtagataaa agaaaggata ctaatatggt attagcgatt attttagtaa
    23881 cattccttat tcgattgatt tttttaaagc gttcgataga gaatgagaaa cgaatcctta
    23941 gcaatggcgg gaaagaattt ggagttgaga atacaaaacg attaactatg gctcatattg
    24001 ttttttatct ctcttgcttg gttgaggcaa tggtgcacaa gacaattttt gatagcatgg
    24061 gcatggttgg tttagtcttg cttatttttt ctatgctgat gttaatgttg gtgattcact
    24121 tgttgggaga tatttggaca gtgaagctta tgcttgtcaa taatcacaaa tatgtagatc
    24181 atatcttgtt taggacagta aaacatccta attacttttt aaatattctt cctgagttga
    24241 ttggcttgac cttgttgagt catgcctata tgacttttgt tttagttttt ccagtttatg
    24301 cagttatttt gtatcgacga atagctgaag aggaaaagct attacatgaa gttataatcc
    24361 caaatggaag cataaagaga taaatacaaa attcgatt
//
LOCUS       DAVGJL010000015        24158 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.015, whole genome shotgun sequence.
ACCESSION   DAVGJL010000015 DAVGJL010000000
VERSION     DAVGJL010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24158
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.015"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            20..1235
                     /gene="sstT"
                     /locus_tag="VVE42_000544"
                     /pseudo
     CDS             20..1235
                     /gene="sstT"
                     /locus_tag="VVE42_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            1303..2256
                     /locus_tag="VVE42_000545"
     CDS             1303..2256
                     /locus_tag="VVE42_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEU3072792.1"
                     /translation="MSISQRTTKLILATCLACLLAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAIGVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREEEIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLDEIERYLEVF
                     RNRSLPKTREEFETRATLLQLLREAKTFIQVKVDFYQKYRQ"
     gene            complement(2447..2671)
                     /locus_tag="VVE42_000546"
     CDS             complement(2447..2671)
                     /locus_tag="VVE42_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory secretory protein Asp5"
                     /protein_id="HEU3072793.1"
                     /translation="MIEILIVLAIILSLALIVLVTIQPRQNQLFSMDATSNIGKPSYW
                     QSNTLVKVLTLLVSLALFILLLTFMVITYK"
     gene            complement(2676..2855)
                     /gene="asp4"
                     /locus_tag="VVE42_000547"
     CDS             complement(2676..2855)
                     /gene="asp4"
                     /locus_tag="VVE42_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory secretory system protein Asp4"
                     /protein_id="HEU3072794.1"
                     /translation="MSEEDLFYKDVEGRMEELKQKPIKKEKETRGEKISKTFSLLLGL
                     MILIGLLFTLLGILR"
     gene            complement(2857..4194)
                     /gene="gtfB"
                     /locus_tag="VVE42_000548"
     CDS             complement(2857..4194)
                     /gene="gtfB"
                     /locus_tag="VVE42_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HEU3072795.1"
                     /translation="MIELYDSYSQESRDLHESLVATGLSQLGVVIDADGFLPDGLLSP
                     FTYYLGYEDGKPLYFNQVPVSDFWEILGDNQSACIEDVTQERAVIHYVDGMQARLVKQ
                     VDWKDLEGRVRQVDHYNRFGACFAKTTYSADSEPIMTVYQDVNGQQVLLENHVTGDIL
                     LTLPGQSMRYFANKVEFITFFLQDLEIDTSQLIFNTLATPFLVSFHHPDKSGSDVLVW
                     QEPLYDAIPGNMQLILESDNVRTKKIIIPNKATYERALELTDEKYHDQFVHLGYHYQF
                     KRDNFLRRDALILTNSDQIEQVEAIAGALPDVTFRIAAVTEMSSKLLDMLRYPNVVLY
                     QNASPQKIQELYQLSDIYLDINHSNELLQAVRQAFEHNLLILGFNQTVHNRLYIAPDH
                     LFESSEVAALVETIKLALSDVDQMRQALGKQGQHANYVDLVRYQETMQTVLGG"
     gene            complement(4187..5698)
                     /gene="gtfA"
                     /locus_tag="VVE42_000549"
     CDS             complement(4187..5698)
                     /gene="gtfA"
                     /locus_tag="VVE42_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HEU3072796.1"
                     /translation="MTIYNINLGIGWASSGVEYAQAYRAGVFRKLNLSSKFIFTDMIL
                     VDNIQHLTANIGFDDNQVIWLYNHFTDIKIAPTSVTVDDVLAYFGGEESHREKNGKVL
                     RVFFFDQDKFVTCYLVDENKNLVQHAEYVFKGNLIRKDYFSYTRYCSEYFAPKDNVAV
                     LYQRTFYNEDGTPVYDILMNQGKEEVYHFKDKIFYGKQAFVRAFMKSLNLNKSDLVIL
                     DRETGIGQVVFEEAQTAHLAVVVHAEHYSENATNEDYILWNNYYDYQFTNADKVDFFI
                     VSTDRQNEVLQEQFAKYTQHQPKIVTIPVGSIDSLTDSSQGRKPFSLITASRLAKEKH
                     IDWLVKAVIEAHKELPELTFDIYGSGGEDSLLREIIANHQAEDYIQLKGHAELSQIYS
                     QYEVYLTASTSEGFGLTLMEAIGSGLPLIGFDVPYGNQTFIEDGQNGYLIPSSSDHVE
                     DQIKQAYAAKICQLYQENRLEAMRAYSYQIAEGFLTKEILEKWKKTVEEVLHD"
     gene            complement(5710..8082)
                     /gene="secA2"
                     /locus_tag="VVE42_000550"
     CDS             complement(5710..8082)
                     /gene="secA2"
                     /locus_tag="VVE42_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HEU3072797.1"
                     /translation="MFRRLGQDFQLRKVKKILKQINALKGKMSSLSDQELVAKTVEFR
                     QRLSEGESLDDILVEAFAVVREADKRILGMFPYDVQVMGAIVMHYGNVAEMNTGEGKT
                     LTATMPVYLNAFSGEGVMVVTPNEYLSKRDAEEMGQVYRFLGLTIGVPFTEDPKKEMK
                     AEEKKLIYASDIIYTTNSNLGFDYLNDNLASNEEGKFLRPFNYVIIDEIDDILLDSAQ
                     TPLIIAGSPRVQSNYYAIIDTLVTTLVEGEDYIFKEEKEEVWLTTKGAKSAENFLGID
                     NLYKEEHASFARHLVYAIRAHKLFTKDKDYIIRGNEMVLVDKGTGRLMEMTKLQGGLH
                     QAIEAKEHVKLSPETRAMASITYQSLFKMFNKISGMTGTGKVAEKEFIETYNMSVVRI
                     PTNRPRQRIDYPDNLYITLPEKVYASLEYIKQYHAKGNPLLVFVGSVEMSQLYSSLLF
                     REGIAHNVLNANNAAREAQIISESGQMGAVTVATSMAGRGTDIKLGKGVAELGGLIVI
                     GTERMESQRIDLQIRGRSGRQGDPGMSKFFVSLEDDVIKKFGPSWVHKKYKDYQVQDM
                     TQPEVLKGRKYRKLVEKAQHASDSAGRSARRQTLEYAESMNIQRDMIYKERNRLIDGS
                     RDLEDVVVDIIERYTEEVAADHYASRELLFHFIVTNISFHVKEVPDYIDVTDKTAVRS
                     FMKQVIDKELSEKKELLNQHDLYEQFLRLSLLKAIDDNWVEQVDYLQQLSMAIGGQSA
                     SQKNPIVEYYQEAYAGFEAMKEQIRADMVRNLLMGLVEVTLKGEIVTHFP"
     gene            complement(8075..8515)
                     /gene="asp3"
                     /locus_tag="VVE42_000551"
     CDS             complement(8075..8515)
                     /gene="asp3"
                     /locus_tag="VVE42_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HEU3072798.1"
                     /translation="MIITQRQSIYWGEVGGTYMYGTTVSYYLDKSVRLYNPLLPSGET
                     LKTWFSSVNYQAARTQPQLPLLKRKQEYQLSLVFDCQPENGVYTKITFFDRYGDILEK
                     KVEKAKDFIFTYPEDSYTYQVSLLSAGFESLTFYHFSIKEIRSV"
     gene            complement(8512..10047)
                     /gene="asp2"
                     /locus_tag="VVE42_000552"
     CDS             complement(8512..10047)
                     /gene="asp2"
                     /locus_tag="VVE42_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HEU3072799.1"
                     /translation="MSKELNILQVGLTNWENHYDIPENMNWYHFYPNSSEALREIIEK
                     EDINRFHAVLIEDGQYSRDLFSYVKYFEPYTLFYNQNLQINDREVVDFLKKRCAQAID
                     FLSPQQLINDLSKSLFGGGYGDKLFPPTIQVNPNFTGAISYQGLDYVSLEGEFGQDFA
                     QLAYWAYNIMVQKTLPIELWLEYEKEGNCDFRLVIRKMWSGSVDDFFEEVIVSEKDLE
                     QALFMDSRDGDYFLSISVEARGRGTIKLGNLHQRWSRKQFGKFVLGGNILHDSKRDEI
                     NYFFHPGDFKPPLTVYFAGYRPAEGFEGYFMMKTLGCPFILFSDPRLEGGAFYLGTDE
                     LEGKVKDTITHYLDYLGFDHKDLILSGLSMGTFPALYYGAPFEPHAIIVGKPLANLGT
                     IASRGRLDAPGVSNLAFDCLIHHTGGTSSQDMTELDQRFWKIFKQANFSKTTFGLSYM
                     KDEEMDPQAYEQLVSYLCNTGAKILSKGTAGRHNDDTDTNISWFLHFYRMVLETGFGR
                     EKR"
     gene            complement(10031..11611)
                     /gene="asp1"
                     /locus_tag="VVE42_000553"
     CDS             complement(10031..11611)
                     /gene="asp1"
                     /locus_tag="VVE42_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HEU3072800.1"
                     /translation="MYYFIPAWYGSERTWHADITPWYFSHFRLEFDDTFHQIRLFQEQ
                     DIDSRLLVLAYQPHLRYFLYRHGVLETDTYSVFDVMQDFHNLHTQVLSIRDIEWDDDC
                     EFIYSPFTIIVQKNGKKFAKVEHGVEGFISDIQYFGPNGQIHMHHIMDDRGFISSIIF
                     FEDGQAAYQEYLNPKGVWQFRERLKEGGQVEVNPILGYRFKMLTYQNMGDLVAEFFEN
                     YLQTYVKDQDIFMLPSHSHHDQLVLDRLPSTNPKLLSLFIGRNSQDTFRDLDVTFEKS
                     DLILVDREDSLRLLQELYPERMHQCYHLSSFDTRLRLGRSQTKKESIIYFQLDFEQGI
                     DNQALLQVLSFVAENKDTEVIFGAFAASQEQMNEVEGIVESFIQENIQSENLGKAIDY
                     GDAENPLEENQHQDLRLQFVNLNDELDLIKTLEFVRLIVDLNRHPHLYTQIAGISAGI
                     PQINLVETVYVEHLKNGYLLTDVTEFSKAAHYYTDRLKEWNEALIYSIDKIKEHTGQQ
                     FLGKLEKWIEEVKNVKGT"
     gene            complement(11621..12838)
                     /gene="secY2"
                     /locus_tag="VVE42_000554"
     CDS             complement(11621..12838)
                     /gene="secY2"
                     /locus_tag="VVE42_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HEU3072801.1"
                     /translation="MTKIYSSIAVKKGLFTLFLLFIYVLGSRMTLPFVDLNTKDFLGG
                     STAYLAFSAALTGGNLRSLSIFSVGLSPWMSAMILWQMFSFSKRLGLTSTSIEIQDRR
                     KMYLTLMIAVIQSLAVSLRLPVQSSYSAILVVLMNTILLIAGTFFLVWLSDLNASMGI
                     GGSIVILLSSIVLNIPQDVIETFKLVYIPTGIVVLLVFMTIIFSYLLALMYRARYLVP
                     VNKIGLHNRFKRYSYLEIMLNPAGGMPYMYVMSFLSVPAYLFILLGFIFPNHSGLAAL
                     SKEFMVGKPLWVYVYISVLFLFSIIFAFVTMNGEEIADRMKKSGEYIYGIYPGADTSR
                     FINRLVLRFSVIGGLFNVIMAGGPMLFVLFDEKLLRLAMIPGLFMMFGGMIFTIRDEV
                     KALRLNETYRPLI"
     gene            complement(12895..13572)
                     /locus_tag="VVE42_000555"
     CDS             complement(12895..13572)
                     /locus_tag="VVE42_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU3072802.1"
                     /translation="MGISVAKGEYITIVDSDDIVKENMIETLYQQVQEKDADVVIGNY
                     YNYDESDGNFYFYVTGQDFCVEELAIQEIMNRQAGDWKFNSSAFILPTFKLIKKELFN
                     EVHFSNGRRFDDEATMHRFYLLASKIVFINDNLYLYRRRSGSIMRTEFDLSWARDIVE
                     VFSKKISDCVLAGLDVSVLRIRFVNLLKDYKQTLEYHQLTDTEEYKDICFRLKLFFDA
                     EQRNGKS"
     gene            complement(13557..13799)
                     /locus_tag="VVE42_000556"
     CDS             complement(13557..13799)
                     /locus_tag="VVE42_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU3072803.1"
                     /translation="MSELISVVVPIYNTGKYLVECVEHILKQTYQNIEIILVDDGSTD
                     NSGEICDAFMMQDNRVRVLHQENKGGQHKLKIWGLV"
     gene            complement(13799..14995)
                     /locus_tag="VVE42_000557"
     CDS             complement(13799..14995)
                     /locus_tag="VVE42_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEU3072804.1"
                     /translation="MRKSIVLAADNAYLIPLETTIKSVLYHNRDVDFYILNSDIAPEW
                     FKLLGRKMEVVNSTIRSVHIDKELFESYKTGPHINYASYFRFFATEVVESDRVLYLDS
                     DIIVTGELATLFEIDLKGYSIGAVDDVYAYEGRKSGFNTGMLLMDVAKWKEHSIVNSL
                     LELAAEQNQVVHLGDQSILNIYFEDNWLTLDKTYNYMVGVDIYHLAQECERLDDNPPT
                     IVHYASHDKPWNTYSISRLRELWWVYRDLDWSEIAFQRSDLNYFERSNQSKKQVMLVT
                     WSADIKHLEYLVQRLPDWHFHLAAPCDCSEELTSLSQYTNVTVYQNVLHSRIDWLLDD
                     SIVYLDINTGGEVFNVVTRAQESGKKIFAFDITRKSMDDGLYDGIFSVERPDDLVDRM
                     KNIEIE"
     gene            complement(15004..16224)
                     /locus_tag="VVE42_000558"
     CDS             complement(15004..16224)
                     /locus_tag="VVE42_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HEU3072805.1"
                     /translation="MRNTKRAVVFAGDYAYIRQIETAMKSLCRHNSHLKIYLLNQDIP
                     QEWFSQIRIYLQEMGGDLIDCKLIGSQFQMNWSNKLPHINHMTFARYFIPDFVTEDKV
                     LYLDSDLIVTGDLTDLFELDLGENYLAAARSCFGAGVGFNAGVLLINNKKWGSETIRQ
                     KLIDLTEKEHENVEEGDQSILNMLFKDQYSSLEDQYNFQIGYDYGAAAFKHQFIFDIP
                     LEPLPLILHYISQDKPWNQFSVGRLREVWWEYSLMDWSVILNEWFSKSVKYPSKSQIF
                     KLQCVNLTNSWCVEKIDYLAEQLPEVHFHIVAYTNMANELLALTRFPNVTVYPNSLPM
                     LLEQIVIASDLYLDLNHDRKLEDAYEFVLKYKKPMIAFDNTCSENLSEISYEGIYPSS
                     IPKKMVAAIRSYMR"
     gene            complement(16255..18699)
                     /locus_tag="VVE42_000559"
     CDS             complement(16255..18699)
                     /locus_tag="VVE42_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_1767 family glycosyltransferase"
                     /protein_id="HEU3072806.1"
                     /translation="MIKTIVLAGDRNYIRQLETTIKSILYHNRDVKIYILNQDIMPDW
                     FRKPRKIARMLGSEIIDVKLPEQTVFQDWEKQDHISSITYARYFIADYIQEDTVLYLD
                     SDLIVNTSLEKLFSICLEEKSLAAVKDTDGITFNTGVLLINNKKWRQEKLKERLIEQS
                     IVTMKEVEEGRFEHFNGDQTIFNQVLQDDWLELGRAYNLQVGHDIVALYNNWQEHLAF
                     NDKPVVIHFTTYRKPWTTLIANRYRDLWWEFHDLEWNQILQHHIGEFEMTSPLDKEFS
                     CLTLTNSQDLEGIEELVTALPEVVFHIAAWTDMGDKLKKLAVYNNVRLHPQIVPPVLD
                     KLERSVDLYLDINYSHVVGTILEDMKILEKPILSFDTTEHGNTGQLVFKKEEVSVMVQ
                     AIKDYRRDGRFLSCYEGSDFHCLTFTNSQELQKIDYLVKNFPMVTFHIAAWTVMGPML
                     QELAKKYKNVKLYPEVKSDQFEELKSQMDVYLDINLHNSTSEVVKEISSLNKPMLAFY
                     TSQNGNHGQHLYSSEHPERLLQAFHALIKGETLGEPEKPIKVIGIDQTLDYVIKNKSS
                     LVRFGDGEVNLMWGLPIPYQNHDLELANQLKHIVGLESDEKLVVCLPDAFDDRFVFTW
                     WATPFWKEHMNVYMDFYKELCKGSWYGSTFISRPYIDYEDKSKAKGQFEKLKSIWENR
                     DILIVEGITSRSGVGNDLFDKVKSVKRIICPSHNAYSVVDNIQEEIMKHAEGRLILCM
                     LGPTAKVLSYNLCQMGYQVLDVGHIDSEYEWMKTGAKTKVKFSHKHTAEHNFDQDIEF
                     IDDETYNSQIVARILN"
     gene            complement(18709..19719)
                     /locus_tag="VVE42_000560"
     CDS             complement(18709..19719)
                     /locus_tag="VVE42_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEU3072807.1"
                     /translation="MKLHLTNLYGMAGDSTVILAQNAVQKIASQLGFREVGIYFYNIA
                     SDSPSEMNKRLDGIMASISIGDILVFQSPTWNGFEFDRLLFDKLKDMQVKIICFIHDV
                     VPLMFDSNYYLMKDYMYMYNLSDVLIVPSERMKTRLMEEGLTTKKILVQGMWDHPHDL
                     SLYTPAFKKELFFAGSLERFPDLQNWSQDTPLRVFSNKGEASSSARNLSIEGWKKDEE
                     LLLELSKGGFGLVWGTHQNEGESNQYYTLNISHKVSTYLTVGIPVIVPSSLSTAEFIV
                     DQGLGFVANSLEEVHEIVDKMNLQEYQEMTNRIKTFSYLLKEGYFTKKLLVDAIYHLG
                     VE"
     gene            complement(19748..20338)
                     /locus_tag="VVE42_000561"
     CDS             complement(19748..20338)
                     /locus_tag="VVE42_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEU3072808.1"
                     /translation="MEHDALDRLYGALKKENADISIGRYNSYDETRYVYMTYVTDPDD
                     SLEVIEGKAIMDREGVEEVRNGNWTVAVLKLFKRELLQDLPFPIGKIAEDTYWTWKVL
                     LRASRIVYLNRCVYWYRVGLSDTLSNTWSEKRMYDEIGAREEKIAILASSDYDLTNHI
                     LIYKNRLQRVIAKLEEQNMQFTEIYRRMMEKLSLLP"
     gene            complement(20343..20624)
                     /locus_tag="VVE42_000562"
     CDS             complement(20343..20624)
                     /locus_tag="VVE42_000562"
                     /inference="COORDINATES: protein motif:HMM:NF012745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU3072809.1"
                     /translation="MENELISIVVPIYNVENYLRMCLDSIQNQTYQNFECLLINDGSP
                     DHSSKICEEFVEKDSRFKYFEKANGGLSSARNLGIECSGGGVHYFCRLG"
     gene            complement(20664..21542)
                     /locus_tag="VVE42_000563"
     CDS             complement(20664..21542)
                     /locus_tag="VVE42_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HEU3072810.1"
                     /translation="MKKNHLVGDALILTVSDQIEELDYLLESLPNICFHIAAPVQFSE
                     KIRSLETNYNVRLRTITNEEQLNFLVDTCDFLLDINHFQEVDAIVSKFVQAGKSVFAF
                     DNTVHGNQGQEIFLSSTPDKLVSRVRDYLNEVRVGTNHQEKIIQDGTWNVFKIDDKAH
                     FIVGSNVICRNFENFHVSSGKLILHDGVFINNSCSFNCMERIEIGAGTMMGEGVRFYD
                     HDHIYTAEKIEKWQWTTAPIRVGRDCWIGSNVTILKGVTIGDNTIIGAGCLIRNDIPS
                     NSVVYNNGNLFVKRRD"
     gene            complement(21539..22939)
                     /locus_tag="VVE42_000564"
     CDS             complement(21539..22939)
                     /locus_tag="VVE42_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000543101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEU3072811.1"
                     /translation="MHALVDAMSERITLLANMGYPLEKHLAVYRQMLEFSLSNGQASG
                     LSDTATYKEFEMKKRLLNQLTVQESSEKKAIVLAANYSYVDQVMTTIKSICYHNRSLR
                     FYLIHSDFPNEWIKQLNKRLEKFDSEIINCRVTSEQISCYKSDISYTVFLRYFIADFV
                     QEDKVLYLDCDLVVTKNLDDLFATDLQDYPLAAVRDFGGRAYFGQEIFNAGVLLVNNA
                     FWKKENMTQKLIDLTNEWHDKVDQADQSILNMLFEHKWLELDFDYNHIVIHKQFADYQ
                     LPEGQDYPAIIHYLSHRKPWKDLAAQTYREVWWYYHGLEWTELGQNHHLHPLQRSHIY
                     PIKEPFTCLIYTASDHIEQIETLVQSLPDIQFKIAARVIVSDRLAQMTIYPNVTIFNG
                     IHYLVDVDNELIETSQVLLDINHGEKTEEILDQFANLGKPILSFENTKTYEVGQEAYA
                     VDQVQAMIEKLREISK"
     gene            complement(22923..23600)
                     /locus_tag="VVE42_000565"
     CDS             complement(22923..23600)
                     /locus_tag="VVE42_000565"
                     /inference="COORDINATES: protein motif:HMM:NF025025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU3072812.1"
                     /translation="MDDKITVIVPVYNVENYLRKCLDSIISQTYKNIEIVVVNDGSTD
                     ASGEICKEFSEMDHRILYIEQENAGISAARNTGLNNMSGNYVTFVDSDDWIELDYVET
                     LYKKITEYQADIAVGNYYSFNESEGMFYFHISGDSYYEKVYDNVSIFENLYETQEMRS
                     FALISAWGKLYKARLFEQLRFDMGKLGEDGYLNQKVYLLSEKVIYLNKGLYAYRIRNR
                     KVDARLS"
     gene            complement(23661..>24158)
                     /locus_tag="VVE42_000566"
     CDS             complement(23661..>24158)
                     /locus_tag="VVE42_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU3072813.1"
                     /translation="SASTSASASASTSASASSSTSASESASTSASASESASTSASESA
                     STSASASASTSASASASTSASASASTSASASASTSASESASTSASASASTSASASAST
                     SVSNSANHSNSQVGNTSGSTGKSQKELPNTGTESSTGSVLLGVLAAVTGIGLVAKRRK
                     RDEEE"
BASE COUNT         7540 a         5228 c         3839 g         7551 t
ORIGIN      
        1 acacaaaaag gagattatta tgaaaagaat cattcatgct tggaataagg caagccttgt
       61 caatcgtatc ttgattggta tgctttgtgg ggaatcctag gactgaccct tcctaatctc
      121 tcagggattg ggctactcga ggatttattc gttggaggtc taaaagctgt tgctcctatc
      181 ctagtctttg ctctcgttgc caatgccctt tcccaacatc aaaagggaca agatagcaat
      241 atgaaaactg ttgttttctt gtatatccta tgatagggac tttcgccgct gctcttgtag
      301 ctgtactagc aagtttcatc gtccctattg aaattaccct aaatagtgcc aatactgaaa
      361 ttgcaccacc agatgggatt gggcaggtcc tcagcaacct cttgctcaaa ctggttgaca
      421 acccagtcaa cgccctgctt actgctaact atattggaat cttatcttgg gcagtcattt
      481 ttggaatcgc tatgagagaa gccagtaaaa atagtaaaga attgctaaaa actatcgctg
      541 acgtgacttc taaaattgtc gaatggatca tcaatctggc tccatttgga atccttggtc
      601 ttgtttttaa aaccatttct gacaagggag tcggaagcct tgccaactac ggtattttat
      661 tggttccatt agtaacgact atgctttttg ttgcccctgt ggtcaaccct ttgattgcct
      721 tcttctttat gagtatgaga cgcaatcctt accctctagt ttggaactgc ctccgtgtca
      781 gcggtgtgac agcctttttc actcgtagtt ctgcgactaa cattcctgtc aacatgaaac
      841 tctgccatga ccttggactc aacccagata cctattctgt ttctatccca ctcggttcta
      901 ctatcaatat ggctggagta gcgattacca ttaacgtttt gacccttgtt acagttaaca
      961 ctcttggaat tcctgttgac tttgccacag cctttgtcct cagtgtggta gcagctatct
     1021 cagcctgtgg tgcttcaggt attgccggag gttccctcct tcttatccca gttgcttgta
     1081 gccttttcgg tatttctaac gatattgcca tacaagttgt tggggttggt tttgtgattg
     1141 gtgtcatcca agactcatgt gaaacagccc ttaactcttc tacagatgtc ctctttaccg
     1201 ccgttgccga atacgcagca acccgtaaaa aataactcat caaggcaagc ctgctcatgt
     1261 cttgtctttt acgcttttat tctaacttat taggaaattc ttatgtctat tagccaacgt
     1321 acgaccaagc tcatcttagc tacctgtctt gcctgcctgc ttgcttattt tctcaatctt
     1381 tcgtcagcag tttcggctgg aattatcgct ctcttgagcc tatctgatac gcgtagaagt
     1441 actttaaaac tggctcgcaa tcgtcttttt tctatgcttc tagctctggc tatcggtgtt
     1501 ctagcttttc acttgagcgg atttcatatc tggagtctcg gcctctatct ggccttctac
     1561 gttcctttag cctacaagat gggctgggaa attggcatca caccaagcac ggttttggtt
     1621 agccatctct tggttcaaga gtcaacctct ccagacctcc tagtcaacga attccttctc
     1681 tttgctattg gtacaggatt tgccttgctt gttaatctct atatgccttc acgagaagag
     1741 gaaatccagc actaccacac gctggtggaa gaaaagttaa aagatatcct ccagcgcttc
     1801 aaatactatt tatccagagg agacggacgc aaccgagcac agctggtagc agaattagac
     1861 acgcttttga aagaagccct cagactggtc tatttggatc actctgacca cctctttcac
     1921 cagaccgact accatatcca ctactttgag atgagacaac gacaaagtcg tatcctgaga
     1981 aacatggccc aacagattaa cacttgtcac cttgccgcca gtgaaagcct gatcttagcg
     2041 caactctttt caaaaattgc aggtcaactg agccagacca atcctgcttc tgatttgcta
     2101 gatgaaattg aacgttatct ggaagtcttc cggaaccgca gtctgcccaa gacaagagaa
     2161 gaatttgaaa cccgcgccac ccttcttcaa ctcctacgtg aagccaaaac cttcatccaa
     2221 gtaaaagttg atttttacca aaaatataga cagtaatact cttcaaacca cgtcagcgtc
     2281 gccttgctgt atatatgtta ctgacttcgt cagttctatc cacaacctca aaacggtgtt
     2341 ttgagcagcc tgcggctagc ttcctagttt gctctttgat tttcattgag tataaaaaag
     2401 aaaacttcag accaatccac aaaaggtgaa tatctgaagt tttcttttat ttataagtaa
     2461 tcaccataaa ggttaatagt agaataaata aagccaaact caccaataaa gtgagcacct
     2521 tgaccaaggt gttgctctgc cagtagcttg gtttaccaat attactagtg gcatccatgg
     2581 aaaatagttg attttgacgg ggttgtatag ttaccaatac aatcaaagca agagatagga
     2641 taatagctaa aacaattagt atttcaatca tagatctacc tcaaaattcc cagcaaagta
     2701 aagagcaaac caatcagaat cattaagccc agtaaaagtg aaaaagtttt actaatcttc
     2761 tcccctcggg tttctttttc cttcttgatg ggtttttgtt tcaactcttc catgcggcct
     2821 tcaacgtctt tgtaaaataa atcttcctct gacatgttag cctcctaaaa cagtttgcat
     2881 ggtttcctga tatctcacca agtcaacata atttgcatgt tggccttgtt tgccaagtgc
     2941 ctgacgcatt tgatcaacat ctgaaagggc caatttaatg gtctcaacca aagcagcaac
     3001 ttcactactt tcaaatagat ggtctggagc gatataaagt ctattgtgca ccgtctgatt
     3061 aaagccaaga atcaagagat tgtgctcaaa ggcctgacgc actgcctgta gcaactcatt
     3121 actgtggttt atatccaagt aaatatccga cagttgatac agctcctgaa tcttctgtgg
     3181 actagcgttc tggtaaagga ccacattagg atagcgaagc atgtctaaga gcttagaaga
     3241 catctctgtc accgctgcaa tacggaaagt gacatcaggc aaggctcctg cgattgcttc
     3301 tacttgctca atctgatctg aattggtcaa gattaaggca tctcgtctta ggaaattatc
     3361 acgtttgaac tggtaatgat aacccaagtg cacaaactga tcatggtatt tctcgtcagt
     3421 taactctaaa gcgcgctcat aagtcgcctt atttggaatg atgatcttct tagtacgcac
     3481 attatcactt tccaaaatca actgcatatt acctggaatg gcatcataga gaggttcctg
     3541 ccataccaag acatccgagc cagatttatc tggatgatgg aaggaaacca agaaaggagt
     3601 cgctagagta ttaaagataa gctgactggt atctatttcc aaatcttgca aaaagaaggt
     3661 gataaattca actttatttg caaagtaacg catggactga ccaggcaaag tcaataagat
     3721 atcacccgtc acatggtttt ccagtaaaac ttgttggcca ttgacatctt ggtaaactgt
     3781 cataatcggc tcgctatctg cgctataagt ggttttagca aagcaagcac caaagcgatt
     3841 gtagtggtca acctgacgta ctcgaccttc taggtctttc cagtctacct gtttaaccaa
     3901 gcgagcctgc attccatcaa cataatgaat gacagccctc tcctgcgtca catcttcaat
     3961 acaagcagac tgattatctc ctaaaatttc ccaaaaatct gaaacgggaa cttggttaaa
     4021 atagagaggt tttccatcct cgtaacctag ataataggta aaaggagaaa gcagaccatc
     4081 aggcagaaaa ccatctgcat cgatgaccac tccaagttga gaaagaccag tagcgactag
     4141 actttcatgt aaatctcgac tttcttgact gtaactatca taaagttcaa tcatggagca
     4201 cctcctctac tgttttcttc cacttttcta aaatttcttt ggtcaagaaa ccttctgcaa
     4261 tttggtaaga ataggcacgc atagcttcca aacgattttc ttgatacaat tgacaaatct
     4321 tagcggcata agcttgcttg atttggtctt ctacatggtc agatgaactt ggaatcaaat
     4381 aaccattttg cccatcctct ataaaggtct gattaccata aggcacatca aaaccaatta
     4441 gaggtagacc tgaaccaata gcttccatca aggtcagacc aaatccttcg ctggtagaag
     4501 ccgttaagta gacctcatac tggctataaa tctgcgaaag ttccgcatgc cccttgagtt
     4561 ggatatagtc ctctgcctga tgatttgcaa taatttctct aagcagagaa tcttctccac
     4621 cactaccata gatatcaaag gttagttccg gtaactcctt atgagcttca atcacagctt
     4681 tcacaagcca atcaatgtgt ttttctttgg caagacgtga agccgtaatc aatgaaaatg
     4741 gtttgcgccc ttgacttgaa tctgtcaagg aatcaatact gcctacagga atcgtaacaa
     4801 tctttggttg atgctgagta tatttggcaa attgctcttg tagaacttca ttttgtctat
     4861 cagtagacac gataaagaag tcaaccttat ctgcattggt aaactggtag tcgtaatagt
     4921 tgttccaaag gatatagtcc tcgtttgtag cattttcact ataatgctcc gcatgaacaa
     4981 ctaccgctaa atgtgctgtc tgtgcttcct caaacacaac ctgaccaatg cctgtctccc
     5041 tatcgagaat gaccaaatca gacttattca aattcaaaga tttcataaag gcacgcacaa
     5101 aagcttgctt tccatagaaa atcttatcct tgaaatgata aacttcttcc ttcccttgat
     5161 tcatcaagat atcatagact ggagtcccgt cttcattgta aaaagttcgt tggtataaga
     5221 ctgcaacatt gtccttggga gcaaaatact cgctacaata acgcgtataa gaaaagtaat
     5281 ccttccgaat caggtttccc ttaaaaacat actcggcatg ttgaaccaag ttcttgttct
     5341 catcaactaa ataacaggtt acaaacttat cttggtcaaa aaagaagaca cgtaaaacct
     5401 tgccattttt ttctctgtga ctttcttcac caccaaagta agccaagaca tcatccactg
     5461 tcacgctagt aggtgcaatt ttgatatctg tgaaatgatt ataaagccag ataacctgat
     5521 tatcatcaaa accaatattg gctgttaagt gctgaatatt atcgactaaa atcatatctg
     5581 taaagataaa cttagaggac agatttaatt tccgaaaaac accagcacga taggcttgag
     5641 cgtattcaac accgctacta gcccaaccaa ttcctaaatt tatattgtaa attgtcatat
     5701 tttctcctct tatggaaaat gagtcacgat ttcacctttt agagtgacct caaccagccc
     5761 catcaggaga ttacgcacca tatccgcacg gatttgttct ttcatagctt caaagcccgc
     5821 gtaggcttct tgatagtact ctacgattgg atttttctga ctagcagatt gaccaccgat
     5881 agccatggat agctgttgta gatagtctac ctgctctacc cagttgtcat caatggcttt
     5941 aagcagtgaa agtcgtaaaa actgttcata taagtcatgt tgattaagta attctttctt
     6001 ttcagaaagt tctttatcaa tcacctgctt cataaagcta cgaactgcag ttttgtcagt
     6061 tacatctata taatctggaa cctctttaac atgaaaacta atattggtca caataaagtg
     6121 aaacaataat tcacgactag catagtgatc agccgctacc tcttctgtat atctctcaat
     6181 gatatccaca acaacatcct ctaagtcacg agaaccatct attagacgat tacgctcctt
     6241 gtaaatcata tcccgttgaa tattcatact ttcagcatat tctagagtct gacgacgtgc
     6301 cgaacggcca gcactatcac tagcatgttg agccttttcg actagtttcc ggtatttacg
     6361 acctttcaat acttccggtt gagtcatatc ttgaacctga tagtctttgt actttttatg
     6421 cacccaagat ggaccaaatt tcttgataac atcatcctct aaggatacaa aaaatttact
     6481 cataccagga tctccctgac gaccagaacg gccacgaatt tgtaggtcga tccgctgact
     6541 ttccatccgc tcagtcccaa taacaatcaa gcccccaagc tctgcgactc ctttaccaag
     6601 cttgatatcc gtaccacgtc ctgccataga ggtagccact gtcacagccc ccatctgacc
     6661 tgactcggag ataatctgag cctcacgcgc cgcattatta gcatttagga cattatgggc
     6721 aatcccttca cgaaacaaga gagacgaata gagttgagac atttcaactg agcctacaaa
     6781 aacgagtaag ggatttccct tagcatggta ttgcttgatg tactccaagg atgcatacac
     6841 tttttcaggt aaagtgatat atagattatc tggatagtca atccgttgtc tcggacgatt
     6901 ggttggaatg cgtactacag acatattgta agtttcaata aactcttttt ccgcgacctt
     6961 acctgtccct gtcataccag atatcttatt aaacatctta aaaagactct gataggtgat
     7021 cgatgccata gcccgcgtct caggagataa tttgacatgt tccttggctt caatagcctg
     7081 atggagacct ccttgaagtt tagtcatttc cattagacgc cctgttccct tatcaaccag
     7141 taccatctca tttccacgaa tgatatagtc cttatcttta gtaaagagct tatgagctcg
     7201 aatcgcataa accaaatgac gagcaaaaga cgcatgctct tccttgtata aattatcaat
     7261 ccctaggaaa ttctcagcag acttggcccc cttagtagtg agccaaacct cctctttctc
     7321 ctctttaaag atataatcct ctccttcgac caaggttgtt acaagtgtat caatgatcgc
     7381 atagtaatta gactgaacac gaggagaacc cgcaataatc agaggagttt gtgcactatc
     7441 aagcaagata tcatcaattt catcaataat cacatagtta aacggtcgta aaaacttacc
     7501 ttcttcattc gaggctaggt tatcatttag ataatcaaaa cctaaattac tattggttgt
     7561 gtagatgata tccgaagcat agataagctt cttttcttca gctttcattt ccttctttgg
     7621 atcttccgta aatggtacac caatggtcaa tcctagaaaa cgataaactt ggcccatttc
     7681 ctcggcatca cgctttgata aatactcatt aggagtcaca accatcactc cttctcctga
     7741 aaaagcgttc aaatagacag gcatggtagc tgtcaaggtc ttaccttccc ccgtattcat
     7801 ctcagcaaca tttccatagt gcatgacaat agctcccatg acttgaacat cataggggaa
     7861 catccctaaa atccgcttat ctgcttcacg caccacagca aaagcttcaa ccaaaatatc
     7921 gtctagactt tctccctcgg aaagacgctg acgaaactct actgttttag ctactaattc
     7981 ttgatccgaa agagaggaca tcttgccttt cagggcatta atctgcttta aaatcttttt
     8041 cactttccta agctggaaat cttgacctaa acgtctaaac acttctgatc tccttgatag
     8101 aaaaatgata aaaagttaag gactcaaatc cagcacttaa aagagaaact tgataagtat
     8161 aactatcttc tggataagta aatatgaaat ctttcgcttt ttctaccttt ttttctaaaa
     8221 tatcaccata gcggtcaaaa aaagttatct tggtataaac tccattttca ggctgacaat
     8281 caaaaaccag tgatagttga tactcctgct ttctttttaa taagggaagc tgaggttggg
     8341 ttcgtgcagc ctggtaattg acactagaaa accaagtctt tagagtctca ccagatggca
     8401 atagaggatt atacagacga acacttttgt caaggtaata agatacagtt gttccataca
     8461 tataagtccc accaacttct ccccaataaa tagactgtct ctgtgtaata atcatctttt
     8521 ctcccttcca aaaccagtct ctaagaccat tctataaaag tgcaaaaacc aagaaatatt
     8581 ggtatctgta tcatcattgt gtcgtccagc agttccttta gataaaatct tcgcacctgt
     8641 attacacaga taagacacta attgttcata ggcttgtgga tccatttctt catctttcat
     8701 ataggataaa ccaaaggttg tctttgaaaa atttgcttgt ttaaaaattt tccaaaaacg
     8761 ctgatccaac tccgtcatat cttgagaact tgtcccacct gtatgatgaa ttaaacaatc
     8821 aaaagctaag ttagagacac ccggtgcgtc caaacgtcca cgactagcta tagttcctaa
     8881 attagccaag ggcttaccaa ctatgatggc atggggttca aaaggagcac cataatagag
     8941 agccggaaac gttcccatag aaagacctga taaaatcaaa tccttatgat caaagcctaa
     9001 ataatcaaga tagtgagtga tcgtatcctt tacttttccc tctagctcat ccgttccgag
     9061 ataaaaagct cccccctcta aacgtggatc agaaaataaa atgaagggac atccaagagt
     9121 tttcatcata aagtaaccct cgaatccttc tgcaggtcga taacctgcaa aatagacagt
     9181 caaaggcggt ttaaaatcac ctggatggaa gaaatagttt atttcatcac gcttggaatc
     9241 atgtaggata tttccaccaa gtacaaactt accaaattgt tttcgactcc atcgttggtg
     9301 aagattacct agtttgatag ttccacgacc tcttgcttca acagatatcg agagaaagta
     9361 gtctccgtct cgactatcca taaaaagtgc ttgctccaag tctttttcag atactattac
     9421 ttcttcaaag aaatcatcaa cagacccact ccacatttta cggattacta aacgaaagtc
     9481 acaattgcct tccttctcat attcaagcca caactcaata gggagtgttt tttgcaccat
     9541 aatattataa gcccaatagg caagctgggc aaaatcttgc ccaaactcac cttccaaact
     9601 gacataatcc aatccttgat aagaaatagc tcctgtaaaa tttggattga cttgtatcgt
     9661 cggaggaaag agtttatcac catacccacc gccaaaaaga gatttactta aatcattgat
     9721 tagttgttgg ggacttaaaa aatcaattgc ttgtgcacat cgttttttta gaaaatccac
     9781 aacctctcta tcatttattt gtagattctg gttatagaat aaagtataag gttcaaaata
     9841 ttttacatag gaaaataagt ctctggaata ctgaccatct tctattaaaa ctgcatgaaa
     9901 acggttaata tcctcttttt cgattatttc acgaagagct tctgatgagt ttgggtaaaa
     9961 atgataccaa ttcatatttt caggtatgtc atagtgattt tcccaattgg taagccctac
    10021 ctgtaaaata ttaagttcct ttgacatttt taacctcctc tatccatttc tctaattttc
    10081 caagaaattg ttgtccagtg tgctccttaa tcttatcaat cgaataaatt aaagcctcat
    10141 tccactcttt caaccgatca gtgtaataat gtgcagcctt agaaaattct gtcacatctg
    10201 tcaacaaata accatttttt aaatgttcca catagacggt ttcaactagg ttgatttgag
    10261 gaattcctgc actaatccca gcaatctgtg tgtagagatg aggatgtcta tttaaatcca
    10321 caatcaaacg gacaaattct agtgttttta tcaaatctaa ctcatcattc aagttaacaa
    10381 actgtaagcg taagtcctgg tgttgatttt cttccagagg attttctgca tcaccataat
    10441 caatcgcctt tcccagattt tcggattgaa tgttttcttg gatgaaagac tcaacaatcc
    10501 cttcaacctc attcatttgc tcctgactag cagcaaaggc tccaaaaatc acctcagtat
    10561 ccttattttc agcgacaaag gacaagactt gaagcagagc ttgattatca atcccctgct
    10621 caaaatccag ttgaaaataa atgatggatt ctttcttagt ttggcttcgt cccaatcgta
    10681 atcgggtgtc aaaagatgat aaatgataac attgatgcat tcgttcagga tacaactcct
    10741 gcaacaatcg taaactatcc tctctatcca ccaaaatcaa atccgatttt tcaaaagtta
    10801 catctaaatc cctaaaggta tcttgagaat tacgtccaat gaacagactc aacagtttag
    10861 gattagtact aggtaaacga tctagtacca actggtcatg atgagaatga gaaggaagca
    10921 taaaaatatc ctgatccttc acatacgttt gcagataatt ctcaaaaaat tctgccacca
    10981 gatctcccat attttgatag gtaagcattt taaagcgata acccaaaatt ggattgactt
    11041 ctacctgtcc tccttctttt aaacgctctc taaattgcca aactccctta ggattcaaat
    11101 attcttgata ggctgcttgc ccatcttcaa aaaaaataat actcgatata aaccctcgat
    11161 catccatgat atggtgcata tgtatttgac cattcggtcc aaaatactgt atatcactga
    11221 tgaaaccttc aactccatgt tcaaccttag caaatttctt cccatttttt tgaacgataa
    11281 tcgtaaaggg actataaata aattcacagt catcatccca ctcaatatct ctaatgctta
    11341 aaacttgggt atggagatta tgaaaatctt gcataacatc aaaaacggaa taagtatccg
    11401 tttctaacac accatgtcta tataaaaaat aacgtagatg aggctggtaa gctaatacta
    11461 atagacgaga atctatatct tgctcttgaa agagccggat ctggtgaaag gtatcatcaa
    11521 actctagacg aaaatgagaa aaataccatg gagtgatatc tgcatgccat gttctttctg
    11581 acccatacca agctggaata aaataataca taaaagtctc ctaaatcaaa ggtctatagg
    11641 tctcatttag ccttaaagcc ttgacctcgt ctctaatcgt aaaaatcatg cccccgaaca
    11701 tcataaataa gcctggaatc attgccaatc gtaataactt ttcatcaaac aaaacaaaaa
    11761 gcatgggacc acctgccata atcacattaa agagaccacc tatgactgag aaacgaagga
    11821 ccaatcgatt aataaatcga ctagtatccg cacctggata aataccataa atgtattctc
    11881 cagatttttt catacggtct gcaatctctt ctccattcat cgtaacaaaa gcaaaaatga
    11941 tactaaataa aaataagacc gaaatataaa catagaccca caaaggcttc ccaaccataa
    12001 attcctttga taaagccgct agccctgaat gattagggaa aataaatccc aacaagatga
    12061 acaaataagc tggtacacta agaaaactca tcacatacat ataaggcatc ccacctgcag
    12121 gattcaacat gatttcgaga taagaatagc gtttaaatcg attgtgtaag ccaattttat
    12181 taacaggaac caaatagcga gctcgataca taagggcaag caagtaagaa aatataatgg
    12241 tcataaaaac aagcaagaca acaatccctg tagggatata aaccagttta aaagtttcaa
    12301 taacatcctg agggatattt aaaacaatgc tggataggag aatgactata gaacccccaa
    12361 ttcccatgct cgcatttaaa tctgacaacc aaacaagaaa aaatgttcct gctatcagca
    12421 atattgtatt cattagaaca accaatattg cagaatagga ggattgtact ggcagtctca
    12481 agctaactgc caaggattga atgacagcta tcatcaaggt caggtacatt ttacggcgat
    12541 cttgtatttc tatagacgta gatgttaaac ccaaccgttt agaaaaagaa aacatctgcc
    12601 acaaaatcat agctgacatc caaggggata atccaacaga aaaaattgac aaacttctta
    12661 gatttccacc tgttagggcg gctgagaagg ctagataggc tgttgaacct cctaaaaaat
    12721 ctttagtatt taggtcaaca aaagggagag tcatacgact tcccaataca tagataaaca
    12781 gtagaaataa ggtaaatagt ccttttttta ctgctattga cgaataaatc ttagtcataa
    12841 aacactcccc tcaccttcat tgtttgtgat atggtaaata acaattcttt tatttcaact
    12901 tttaccattt ctttgttctg catcaaaaaa caactttaat ctgaaacaaa tatctttata
    12961 ttcctcagta tctgttaatt gatggtattc taaagtttgc ttataatctt ttaaaagatt
    13021 gacaaatcga atacgcagaa cggagacatc caaaccagcc aagacacaat ccgatatttt
    13081 cttagaaaac acttcaacaa tatctcttgc ccaggaaaga tcaaattccg ttctcatgat
    13141 gcttcctgaa cgtcttctat acagatagag attatcgttt ataaagacga ttttagaggc
    13201 taaaagataa aagcgatgca tagttgcttc atcatcaaag cggcgaccat ttgaaaagtg
    13261 aacttcattg aataattctt ttttaatcaa cttaaatgtc ggcaatataa aggccgagct
    13321 attgaatttc caatctcctg cttgacggtt cataatttct tgtatagcta attcttcgac
    13381 gcaaaaatct tgccctgtta cataaaaata aaaattcccg tcactttcgt cataattata
    13441 gtaattccct ataacaacat ctgcatcctt ttcttggact tgctgataaa gagtttcaat
    13501 catattttct tttacgatat catctgaatc aacaatcgtg atgtactctc ccttagctac
    13561 actaatcccc atatttttag cttgtgctgc ccccccttat tttcttgatg caatactcgc
    13621 acacgattat cttgcatcat aaaagcatca caaatttccc cagaattatc cgtagaaccg
    13681 tcatcaacta aaataatttc tatattttga taggtttgct tcagaatatg ctcgacacac
    13741 tccactaaat attttcccgt attgtatatc ggtaccacaa cactaattaa ttcactcatt
    13801 actctatctc tatattcttc attctatcca ctaaatcatc tggtctctcc acagaaaaaa
    13861 taccgtcata gagtccatca tccatacttt tacgtgtgat atcaaaagcg aagattttct
    13921 tgccactttc ttgtgccctt gtaactacat taaaaacctc tccacctgta ttaatatcta
    13981 aataaactat agaatcgtcc aatagccaat caattctact atgtaataca ttttgatata
    14041 ctgttacatt cgtatactgt gatagagagg tcagctcctc agaacaatca cacggtgcag
    14101 ccaaatgaaa atgccaatca ggtaaccgtt gtactaaata ctctaaatgt tttatatctg
    14161 cactccatgt cacaagcatc acttgttttt tagactgatt gcttctttca aaataattta
    14221 aatcggaacg ttgaaaagca atctctgacc aatccaaatc tctataaacc caccataatt
    14281 cacgtagtct agatatacta tatgtattcc aaggtttatc atgactagca tagtgaacaa
    14341 ttgtaggtgg attgtcatct agacgttcac attcttgagc aaggtgataa atatcaacac
    14401 ccaccatata attatatgtt ttatctaacg ttagccaatt atcctcaaaa taaatattta
    14461 aaatactctg atccccaaga tgaacaactt gattttgctc ggccgctaat tccaataaac
    14521 tattgacaat agaatgttct ttccactttg caacatccat tagtaacata ccagtattaa
    14581 atccagattt tcgtccttca taggcataga catcatcaac agcaccaatt gaatatcctt
    14641 tgagatctat ctcaaacaaa gtagctagtt ccccagttac aatgatatcg gaatccagat
    14701 acaataccct atcagattca accacttctg tcgcaaaaaa tctaaagtaa gaagcataat
    14761 ttatatgagg tcctgtttta tagctttcaa aaagttcttt atcaatgtgt acactgcgaa
    14821 ttgtagaatt cacaacttcc atttttctcc ccaataattt aaaccattca ggagctatat
    14881 cactgttgag aatataaaaa tcaacatctc tattgtgata caatacagac tttatagtcg
    14941 tctctaaagg aataagatag gcattatctg ccgctaatac tattgatttt ctcatactgt
    15001 tctctacctc atgtaagatc tgattgctgc aaccattttt ttcggaatgg agcttggata
    15061 gataccttca tatgaaatct cagaaagatt ttcagagcaa gtattgtcga aagctatcat
    15121 tggttttttg tacttaagca caaactcata tgcatcttct aattttcgat catgattcaa
    15181 atccaaatac aaatctgaag ctattactat ttgttccaat aacattggta aggaatttgg
    15241 atatacggta acattaggaa aacgcgttaa agctagtagt tcatttgcca tatttgtata
    15301 agcaacaata tgaaaatgaa cttctggcaa ttgctctgcc aaataatcga ttttctcgac
    15361 acaccaagaa ttcgttaaat taacacattg caacttaaat atttgtgatt tactagggta
    15421 cttcacactc tttgaaaacc attcatttaa aataacagac caatccatca aagagtattc
    15481 ccaccaaact tctcttagac gtccaacaga aaattgattc caaggcttat cctgagaaat
    15541 atagtgtaaa attagtggca gtggttcgag cggaatatca aaaatgaatt gatgtttaaa
    15601 ggctgccgcc ccataatcat atcctatttg aaaattatat tgatcttcaa gggaactata
    15661 ttgatcttta aacaacatat tcaaaattga ctggtctcct tcttccacat tctcatgttc
    15721 tttttctgtt aagtcaatca atttttgtcg aatagtttca gatccccatt ttttgttgtt
    15781 aatcaagaga acaccagcat tgaagccgac tcctgctcca aagcaagaac gagctgctgc
    15841 caaataattt tcacctaagt ctaattcaaa caaatcggtc aaatcaccag tcacaatcaa
    15901 atcactatct agatagagaa ctttatcttc tgttacaaaa tctggaataa aatagcgtgc
    15961 aaatgtcata tgattgatat gaggtaattt attagaccaa ttcatttgaa actgtgagcc
    16021 aattaacttg cagtcaatca agtcgccccc catctcttgt aaatatattc ttatttgact
    16081 aaaccattcc tgaggaatgt cctgatttag cagataaatt ttcaaatgac tattgtgtct
    16141 acagagtgac ttcatcgccg tttcgatttg tcgaatataa gcgtaatcac ctgcaaatac
    16201 tacagcgcgt ttggtatttc tcattttaaa tccttatcat ttattttaaa tagtctagtt
    16261 taatattcgt gcaacaatct gactgttata ggtttcatca tcaataaatt caatatcttg
    16321 gtcgaaatta tgttctgcag tatgtttatg agaaaattta accttagttt tagctcccgt
    16381 tttcatccat tcatactctg agtcaatatg gcctacatcc aaaacttgat agcccatctg
    16441 gcatagatta taactcagaa cttttgctgt aggacccagc atacataaaa tcagtcttcc
    16501 ttcagcatgt ttcataattt cctcttgaat gttatcgaca acggaataag cattatgaga
    16561 tggacaaata atccgtttca cagatttgac cttatcaaat aaatcatttc caaccccaga
    16621 gcgagatgta atcccctcta caatcagaat atccctattc tcccaaatac tctttagctt
    16681 ttcaaattgt ccttttgcct tactcttatc ttcataatca atgtatggac gcgagataaa
    16741 ggtagagcca taccatgaac ccttacacaa ctctttataa aaatccatat aaacattcat
    16801 atgttctttc cagaatggtg tagcccacca agtaaagaca aaacgatcgt caaaagcatc
    16861 aggtaaacaa accactaatt tctcgtcact ctcaagaccc acaatatgtt ttaattggtt
    16921 agctaactct agatcatgat tttgataagg tataggcaaa ccccacatca aattgacttc
    16981 tccgtctcca aaacgaacta atgaggactt atttttgata acataatcca gagtctgatc
    17041 aattccgata accttgattg gcttctctgg ttctcccaaa gtctctcctt ttatcaatgc
    17101 atgaaatgct tgaagaagcc tttctggatg ctcacttgaa tataaatgtt gaccatgatt
    17161 tccattttgt gaagtataaa atgccagcat tggtttattc aatgatgata tctccttgac
    17221 aacctcacta gtagaattgt gtaggttaat atccaaataa acatccattt gagatttgag
    17281 ttcttcaaat tgatcgcttt tcacttcagg atatagctta acatttttat atttttttgc
    17341 tagttcttgt aacattggtc ccataactgt ccaagctgcg atatggaatg ttaccatagg
    17401 aaaattctta acaagataat ctatcttttg caattcttga gaatttgtaa atgttaggca
    17461 atggaaatca ctaccttcat aacaacttag gaatcttcca tctcttctgt aatctttaat
    17521 cgcttgaacc atgactgaaa cttcttcttt tttgaatact agttggccag tattcccatg
    17581 ttcagtcgta tcaaatgata gaattggctt ttctaagatc ttcatatcct ctaaaatagt
    17641 acctaccaca tgactgtagt taatgtccag atataaatct acacttctct ccagcttatc
    17701 taatacagga ggaacaattt gtggatgcaa tctcacatta ttatatacag ctaatttttt
    17761 taatttatct cccatatccg tccaagctgc gatatgaaat accacctcag gtagagctgt
    17821 aactagctct tctattcctt ctaaatcttg ggaatttgtc aaagttaaac aagaaaattc
    17881 cttatctaga ggcgatgtca tttcaaactc tcctatatgg tgttgtaaaa tttgattcca
    17941 ctccaaatca tgaaattccc accataaatc acggtaacga ttggctatca aggtagtcca
    18001 tggctttctg taggtcgtaa aatgaatcac cactggttta tcattaaaag ccagatgttc
    18061 ctgccaattg ttatacaaag ccacaatatc atgccctact tgtaaattat aagctcgacc
    18121 tagctctaac caatcatctt gtaagacctg attaaaaatc gtttgatcac cattaaaatg
    18181 ctcgaaacgg ccttcttcaa cttccttcat tgtaacaatg ctctgttcaa ttagtcgttc
    18241 ttttaatttc tcttgacgcc attttttatt gttgattaat agaacacctg tattaaatgt
    18301 aattccatct gtatctttaa ctgctgcgag tgatttttct tctaaacaaa tactaaataa
    18361 tttctctaaa gaagtattta caatcaaatc actgtctaaa tataaaaccg tatcttcttg
    18421 gatataatct gcaataaaat atctagcata ggtaatgcta ctaatgtgat cttgcttttc
    18481 ccaatcttga aacacagttt gttcaggtag tttaacatcg ataatctcac tacctaacat
    18541 gcgagctatt tttcgtggtt tgcgaaacca atctggcata atatcttgat ttaaaatata
    18601 aatcttaaca tctcgattgt ggtataaaat agattttatg gttgtttcta actgcctgat
    18661 gtaattacga tcaccagcta atacaattgt tttaatcatc ttgttctctt attcaactcc
    18721 caaatggtaa attgcatcta ccaataattt tttagtaaaa taaccttctt ttagcaaata
    18781 actaaaggtt tttatccgat tcgtcatttc ttgatattct tgtagattca ttttatcaac
    18841 tatctcatga acttcctcta gactattcgc cacaaagcct aagccttgat ctactataaa
    18901 ttcagcagtt gacaagctac ttggtacaat gactgggatg cccactgtca gataggtact
    18961 caccttatga gatatattca aggtatagta ttggttactt tctccctcat tttgatgggt
    19021 tccccagaca aggccaaatc caccctttga taattctagc aacaattcct catctttttt
    19081 ccatccttcg atgctgagat ttctagcact agaactagct tcccctttat ttgaaaatac
    19141 tctcaaaggc gtatcttgag accaattttg taagtctgga aaacgctcta aacttccagc
    19201 aaaaaaaagt tcttttttaa aagcaggggt gtataaggat aaatcatgag gatgatccca
    19261 catcccttga acaagaatct tcttagtcgt caatccttct tccatcaggc gtgttttcat
    19321 tctctctgat ggcactatca aaacatctga tagattatac atgtacatat agtctttcat
    19381 gagataatag ttactatcaa acatgagggg aacaacatca tggataaagc aaataatttt
    19441 cacctgcata tcctttagct tatcaaacaa gagacgatca aattcaaagc cattccaggt
    19501 tggagactga aagactaaga tatcaccaat agagatactg gccataatac catccagacg
    19561 cttattcatt tcagaaggac tatctgaagc aatgttgtaa aaataaatac caacctctct
    19621 aaatcccagt tgactagcta tcttttgaac agcattttga gctaagataa ccgtactatc
    19681 accagccatg ccgtataaat ttgttaaatg taatttcata ttatgctatc tcatttttta
    19741 ttactatcta cggaagtaaa gacaattttt ccatcattct tctataaatc tctgtgaact
    19801 gcatattttg ttcttctaat tttgctatca ctctttgtaa tctattttta taaatcaaaa
    19861 tatgattggt caagtcatag tctgaacttg ctaaaatagc tatcttttct tccctagccc
    19921 caatttcatc atacatacgc ttttcactcc atgtattcga taaagtatca gataaaccaa
    19981 cacggtacca gtaaacacaa cgattcaaat agactatcct cgaagctctt agaagtacct
    20041 tccatgtcca gtaagtatcc tctgcaattt ttcctatagg aaatggtaaa tcttgtagta
    20101 actctctctt aaatagtttc aagacagcta cagtccaatt cccatttctg acttcttcga
    20161 caccttccct atccatgatt gcttttcctt ctatcacttc cagagaatca tctggatccg
    20221 taacataagt catatataca tagcgtgttt catcataaga attataacgt ccgatactaa
    20281 tatctgcgtt ttcctttttc aaagcaccat ataatcggtc taaagcatca tgttccaacc
    20341 aatcatccga gtctacaaaa gtaatgtacg cccccccccg aacattcaat acctaggtta
    20401 cgagctgatg aaagaccgcc gtttgctttc tcaaaatatt tgaaacgaga atctttctct
    20461 acaaattctt cacatatttt ggatgaatga tctggagagc catcattgat taataaacac
    20521 tcaaaatttt gatacgtctg attctgaatg ctatccaaac acattcgcaa ataattctca
    20581 acattgtaga ttggaactac aatactaatt agttcatttt ccatatacta actttcctag
    20641 acttgattta ttctatttcc ctcttaatct cttctcttta caaaaagatt tccattatta
    20701 taaactacac tgtttgatgg aatatcattg cgtatgagac aacctgctcc aataatggta
    20761 ttatccccaa tagtcactcc ttttaaaatc gtgacattac ttcctatcca gcagtctctg
    20821 ccaactctaa tcggcgctgt agtccactgc cacttttcaa tcttttcagc agtataaata
    20881 tggtcgtgat cataaaaacg aacaccctct cccatcatgg taccagctcc gatttcaatt
    20941 ctttccatac agttaaaact acaagagttg ttgataaaaa caccatcatg cagtattaac
    21001 ttaccagaag agacatgaaa attttcaaaa tttctgcaga tgacatttga tcctacaatg
    21061 aagtgagcct tatcatcgat tttaaagaca ttccaagtcc catcttgaat gattttctct
    21121 tgatgattgg tgcctactct gacttcattc agatagtctc tcactctaga aaccaatttg
    21181 tctggtgtac ttgatagaaa aatttcctgc ccctgatttc catggactgt attgtcaaat
    21241 gcaaagacag attttccagc ctgtacaaac ttggagacaa tagcatctac ttcttgaaag
    21301 tgattgatat ctagtaaaaa gtcacaggta tcgactagaa agtttagctg ttcttcattt
    21361 gtaatcgttc ggagacggac attatagttc gtttctaagc tacggatttt ctcggaaaat
    21421 tgaacaggtg ctgcaatatg aaaacagata tttggcaagg attcaaggag ataatccaac
    21481 tcttcaatct gatcactaac cgtcaaaatc agagcatctc ctactaaatg atttttcttc
    21541 atttgcttat ttctctcaat ttttcaatca ttgcttgaac ttggtcaaca gcatatgcct
    21601 cctgacctac ttcataggtt ttagtatttt caaaggataa gataggcttg ccaagattag
    21661 caaattgatc gagaatttct tctgtctttt cgccatgatt aatatctaaa agtacttgac
    21721 tggtttctat caattcatta tcgacatcta ccaaatagtg aattccgtta aatatagtca
    21781 cgtttggata aattgtcatc tgagccaatc gatcactaac tattactcta gctgctatct
    21841 taaactgaat atcaggcaag gattgaacca atgtctcaat ttgttcaata tggtctgagg
    21901 cagtatagat tagacaagtg aaaggttcct ttattggata gatgtgagat ctttgtaatg
    21961 gatgtaaatg atggttttgt cccaattctg tccattcaag cccatgatag taccaccaaa
    22021 cttcacgata ggtttgggcc gccaaatctt tccacggttt ccgatgagaa agatagtgaa
    22081 taatagcagg ataatcctga ccctcaggca attgataatc agcaaactgt ttatgaatga
    22141 caatatgatt ataatcaaag tccaattcca accatttatg ttcaaaaagc atattcaaga
    22201 tgctctgatc tgcctgatcc accttatcat gccattcatt ggttaaatca attaattttt
    22261 gggtcatatt ctcttttttc caaaaagcat tgtttaccaa gagaacaccg gcattaaaga
    22321 tttcttgacc aaaataagct ctgcccccaa aatctctaac agcagccaaa ggataatctt
    22381 gtaagtctgt agcaaacaag tcatccagat ttttcgttac aactagatca cagtccaagt
    22441 agaggacctt gtcttcttgc acgaaatcag ctatgaaata gcgtaaaaag actgtgtaac
    22501 taatatccga tttataacat gaaatttgct cagaagttac ccgacaatta ataatttctg
    22561 agtcaaactt ctctaagcgc ttatttaatt gcttaatcca ttcatttgga aaatcgctat
    22621 gaatcaaata aaaacgaagc gagcgattgt ggtaacagat agacttgata gtagtcatta
    22681 cttggtctac ataggaatag tttgctgcta ggacaatggc tttcttttca cttgattctt
    22741 gtaccgttaa ctgatttaaa agtctttttt tcatttcaaa ctctttatac gttgctgtgt
    22801 cagataaacc gctagcttga ccgttggaga gactgaattc caacatctga cgataaactg
    22861 ccaaatgttt ctctagagga tatcccatat tagctagtag cgtaatacgt tcagacatag
    22921 catcaactaa ggcgtgcatc cacttttctg tttctaatcc gataagcata aagaccttta
    22981 tttaaataaa ttaccttttc tgataataaa tatacctttt gattgaggta accatcttct
    23041 cctaatttac ccatgtcaaa gcgcaactgc tcaaacaatc tcgccttata gagtttaccc
    23101 caagcagata tcaaagcgaa actcctcatt tcttgagttt catacaaatt ctcaaatatg
    23161 gaaacattgt catatacctt ctcataatag gagtctcctg atatatgaaa gtagaacatt
    23221 ccttcacttt cgttgaaaga atagtaattc ccaactgcaa tatcagcttg atattctgtt
    23281 atttttttat atagggtttc tacataatct aactcaatcc aatcatccga gtccacaaag
    23341 gtcacataat ttccggacat attattcaga ccggtgtttc gtgcggcaga aataccagca
    23401 ttttcttgtt ctatatagag aattcggtga tccatttctg aaaattcttt acaaatttca
    23461 cctgaagcat ccgtagaacc atcattaacg acaacaatct caatattttt atatgtttga
    23521 gaaataatac tatctaggca cttcctcaga tagttttcca cattgtatac tggtacaatg
    23581 accgttattt tatcatccac tagcaatttt ctccttgatt tatgtgcacg agttagttta
    23641 gcctaacttt acaggttgtc ttactcttct tcatcacgtt tacggcgttt cgcaaccaat
    23701 ccaatacctg taacagctgc tagaactcca agtaacacag atccagttga cgactcagta
    23761 cctgtattag gcaattcttt ttgggattta cctgtcgatc cagaagtatt tccaacttgt
    23821 gagttcgaat ggtttgctga atttgaaact gatgtacttg ctgaagctga cgcacttgtc
    23881 gacgctgaag ccgaagcact ggttgaggcc gattcagacg ctgaggtact tgctgaggcc
    23941 gaagcactgg tacttgcgga agctgaggcg ctagtacttg ctgaagccga agcactggtg
    24001 cttgctgaag ccgaggcact tgttgatgcg gattcagagg cactggttga cgctgattca
    24061 gacgctgagg cactggttga cgctgattca gatgctgagg tgcttgatga agctgacgca
    24121 cttgtacttg cggaagctga tgcactagta cttgctga
//
LOCUS       DAVGJL010000016        24009 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.016, whole genome shotgun sequence.
ACCESSION   DAVGJL010000016 DAVGJL010000000
VERSION     DAVGJL010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24009
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.016"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            377..2530
                     /locus_tag="VVE42_000567"
     CDS             377..2530
                     /locus_tag="VVE42_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEU3072814.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRERFEEEWTGVTLFMAPSPDYKPHKEQKNGLLSFIPI
                     LVKQRGLIANIVLATLLVTVINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIL
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQT
                     AQVANNRLNEVYLVASEFEEKKTVEDLSLMKGDMTFKQVHYKYGYGRDVLSDINLTVP
                     QGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGSVNLNQIDKKALRQYINYLPQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLMALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
     gene            2543..3892
                     /locus_tag="VVE42_000568"
     CDS             2543..3892
                     /locus_tag="VVE42_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEU3072815.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQGGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQAEIGNLISQTEAKIRDYQTAKSAIETGASLAGQNLAYSLYQSYK
                     SQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKITASEDGVLHLNPETSDSSMVAEG
                     TLLAQLYPSLEREGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAGNQLFLDSTITSID
                     ATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            4062..4769
                     /locus_tag="VVE42_000569"
     CDS             4062..4769
                     /locus_tag="VVE42_000569"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEU3072816.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQVAG
                     KGVLNNQISSFIFEKLNAAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIADDQQIAYLKEETRRINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQELK"
     gene            4971..8696
                     /locus_tag="VVE42_000570"
     CDS             4971..8696
                     /locus_tag="VVE42_000570"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEU3072817.1"
                     /translation="MDKRIFVEKKADFQVKSESLVREFQHNLGLSSLKSIRIVQVYDV
                     FDLAEDLFAPAEKHIFSEQVTDHVLDEVSVQADLANYAFFAIESLPGQFDQCAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDATELEAVKNYLLNPVDSRFKDITTGIAKQEFS
                     ESDKIIPKLTFFESYTAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDTFVA
                     ECNKENIDAVVVATVTEKPNLVMHWNGETIVDLERRFLDTNGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTIFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEVTARLVAAGANWSKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFAQFEAIQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLPELKTALTAQLGGFVFTSPEEIAGVEKVG
                     QTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVASDVVIKAKE
                     KVEKPVVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSAADEPDGSAKFIVNILLNEKVRVAIDSFIARGGLIIGICNGFQALVK
                     SGLLPYGNFEAANSTSPTLFYNDANQHVAKMVETRIANTNSPWLAGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            8789..10231
                     /locus_tag="VVE42_000571"
     CDS             8789..10231
                     /locus_tag="VVE42_000571"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEU3072818.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGAGAIGHVRYATAGEASVDNIQPFLFR
                     FHDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWI
                     RDLKPGEIVIIDDEGIQYDSYTDDTQLAICSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETCQIIGADSLTYLSIDSLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            10268..11290
                     /locus_tag="VVE42_000572"
     CDS             10268..11290
                     /locus_tag="VVE42_000572"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEU3072819.1"
                     /translation="MTNKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLASSGLHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKALEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            11287..11832
                     /gene="purN"
                     /locus_tag="VVE42_000573"
     CDS             11287..11832
                     /gene="purN"
                     /locus_tag="VVE42_000573"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEU3072820.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKADYEAALVELLEEHQIDLVCLAGYMKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYPEVVKALFTD"
     gene            11916..12425
                     /locus_tag="VVE42_000574"
     CDS             11916..12425
                     /locus_tag="VVE42_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEU3072821.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEILFNLISFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEC
                     LQYILAIGITDITDLTLNTLGVCVGLLIYQIFIRVFKSQTRKWINILGMLSLGFAYLV
                     LLLLHLIGV"
     gene            12450..13997
                     /gene="purH"
                     /locus_tag="VVE42_000575"
     CDS             12450..13997
                     /gene="purH"
                     /locus_tag="VVE42_000575"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEU3072822.1"
                     /translation="MTKRVLISVSDKAGIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     DTIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAAKDNKIELIDLVVVNL
                     YPFKETILKPDVTYADAVENIDIGGPSMLRSAAKNHASVTVVVDPADYAVVLDELAAN
                     GETSYETRQRLAAKVFRHTAAYDALIAEYFTAQVGESKPEKLTLTYDLKQPMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWDYAYESDPVSIFGGIVVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQA
                     KDRLDGAVLASDAFFPFADNVEEIAKAGIKAIIQPGGSVRDQESIEAADKYGLTMVFT
                     GVRHFRH"
     gene            14119..15381
                     /gene="purD"
                     /locus_tag="VVE42_000576"
     CDS             14119..15381
                     /gene="purD"
                     /locus_tag="VVE42_000576"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEU3072823.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLEL
                     VNISISEHSKLIDFAKTNDVAWTFIGPDDALAAGIVDDFNQAGLKAFGPTRAAAELEW
                     SKDFAKEIMVKYGVPTAIYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFLEGEEFSLFAFVNGDKFYIMPTAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPVLEGVIKEGRPYLGVLYAGLILT
                     ADGPKVIEFNARFGDPETQIILPRLTSDFAQNITDILDSKEPNIMWTDKGVTLGVVVA
                     SKGYPLDYERGVELPAKTEGDVITYYAGAKFAENSRALLSNGGRVYMLVTTADTVKEA
                     QASIYQELSQQKIEGLFYRTDIGSKAIK"
     gene            15784..16272
                     /gene="purE"
                     /locus_tag="VVE42_000577"
     CDS             15784..16272
                     /gene="purE"
                     /locus_tag="VVE42_000577"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEU3072824.1"
                     /translation="MKPVISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVVSAHRTPD
                     LMFKHAEEARSRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALFALRLLSVEDKSIADALANFAEEQGKIAEESSNE
                     LI"
     gene            16259..17350
                     /gene="purK"
                     /locus_tag="VVE42_000578"
     CDS             16259..17350
                     /gene="purK"
                     /locus_tag="VVE42_000578"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEU3072825.1"
                     /translation="MSSSKTIGIIGGGQLGQMMATSAIYMGHKVIALDPAADCPASRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAQVTVAPYKVVTSSLDLADIDLSKNYVLKTATGGYDGHGQKVIRSEAD
                     LEAAYALADSADCVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPA
                     RISESLADKAKAMAVRIAEQLNLSGTLCVEMFATDDDVIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPVIKLHAPAVMLNVLGQHVEAAEQYVTENSSAHLHMYGKIE
                     AKHNRKMGHVTLFSDVPDSVDELGEGIDF"
     gene            17360..17587
                     /locus_tag="VVE42_000579"
     CDS             17360..17587
                     /locus_tag="VVE42_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HEU3072826.1"
                     /translation="MIQIIVNTFIEKDKTGAVVEVLYASADQDKVQAKYEELAAQYPE
                     NYLAIYDVPLDTDLNTLDHYPSVFIEKEEFE"
     gene            17650..18948
                     /gene="purB"
                     /locus_tag="VVE42_000580"
     CDS             17650..18948
                     /gene="purB"
                     /locus_tag="VVE42_000580"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEU3072827.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEIDEIFNPVYYTKRVDDIFERLGLGD"
     gene            complement(19003..22941)
                     /gene="strH"
                     /locus_tag="VVE42_000581"
     CDS             complement(19003..22941)
                     /gene="strH"
                     /locus_tag="VVE42_000581"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEU3072828.1"
                     /translation="MKHEKQQRFSIRKYAVGAASVLIGFAFQAQTVAADGVTTTTTEN
                     QPTIHTVSDSPQSSENRTEETPKAELQPEAPKTVETETPATDKVASLPKTEEKPQEEV
                     SSTPSDKAEVVTPTSAEKETANKKEEEASPKKEEAKEVDSKESNTDKTDKDKPAKKDE
                     AKAEADKPETETGKERAATVNEKLAKKKIVSIDAGRKYFSPEQLKEIIDKAKHYGYTD
                     LHLLVGNDGLRFMLDDMSITANGKTYASDDVKRAIEKGTNDYYNDPNGNHLTESQMTD
                     LINYAKDKGIGLIPTVNSPGHMDAILNAMKELGIQNPNFSYFGKKSARTVDLDNEQAV
                     AFTKALIDKYAAYFAKKTEIFNIGLDEYANDATDAKGWSVLQADKYYPNEGYPVKGYE
                     KFIAYANDLARIVKSHGLKPMAFNDGIYYNSDTSFGSFDKDIIVSMWTGGWGGYDVAS
                     SKLLAEKGHQILNTNDAWYYVLGRNADGQGWYNLDQGLNGIKNTPITSVPKTEGADIP
                     IIGGMVAAWADTPSARYSPSRLFKLMRHFANANAEYFAADYESAEQTLNEVPKDLNRY
                     TAESVAAVKEAEKAIRSLDSNLSRAQQDTIDQAIAKLQETVNNLTLTPEAQKEEEAKR
                     EVEKLAKNKVISIDAGRKYFTLNQLKRIVDKASELGYSDVHLLLGNDGLRFLLDDMTI
                     TANGKTYASDDVKKAIIEGTKAYYDDPNGTALTQAEVTELIEYAKSKDIGLIPAINSP
                     GHMDAMLVAMEKLGIKNPQAHFDKVSKTTMDLKNEEAMNFVKALIGKYMDFFAGKTKI
                     FNFGTDEYANDATSAQGWYYLKWYQLYGKFAEYANTLAAMAKERGLQPMAFNDGFYYE
                     DKDDVQFDKDVLISYWSKGWWGYNLASPQYLASKGYKFLNTNGDWYYILGQKPEDGGG
                     FLKKAIENTGKTPFNQLASTKYPEVDLPTVGSMLSIWADRPSAEYKEEEIFELMTAFA
                     DHNKDYFRANYNALREELAKIPTNLEGYSKESLEALDAAKTALNYNLNRNKQAELDTL
                     VANLKAALQGLKPAATHSGSLDENEVAANVETRPELITRTEEIPFEVIKKENPNLPAG
                     QENIITAGVKGERTHYISVLTENGKTTETVLDSQVTKEVINQVVEVGAPVTHKGDESG
                     LAPTTEVKPRLDIQKEEIPFTTVTRENPLLLKGKTQVITKGVNGHRSNFYSVSTSADG
                     KEVKTLVNSVVAQEAVTQIVEVGTMVTHVGDENGQAAIAEEKPKLEIPSQPAPSTAPA
                     EESKALPQDPAPVVIEKKLPETGTHDSAGLVVAGLMATLAAYGLTKRKED"
     gene            complement(23257..23973)
                     /locus_tag="VVE42_000582"
     CDS             complement(23257..23973)
                     /locus_tag="VVE42_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU3072829.1"
                     /translation="MAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSIT
                     VVRALNDLAKDGYIVRQQGKGTFISRARKHKLVEFSDIELFNAKDDKVTVLSIERGNK
                     LVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRF
                     KLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEHFPTVLQTKITKLESTGQVLEYS
                     ETYKRSDYYKIKFISCDRDH"
BASE COUNT         6510 a         4786 c         5708 g         7005 t
ORIGIN      
        1 tactaaaatt tttttcttta cttaataatg atatgaaatc atttcggttt tgttaacttg
       61 taattcttat attttctagg tagaacgttt gttctgccta tttttttatc caaaaagtgc
      121 agttgggagg gagataggct catttgggaa ggaagtccag tttttgttta gtgattgggg
      181 taagatagtt gttatcagat gagtttatac tcttcgaaaa tcaaattcaa accacgtcaa
      241 cgtcgccttg ccgtatatat gtgactgact tcgtcagtcc tatctacaac ctcaaaacag
      301 tgttttgagc agcctgcggc tagtttccta gtttgctctt tgattttcat tgagtattag
      361 ggaaaaggag atgaatatga aatttgggaa acgtcactat cgtccgcaag tggatcagat
      421 ggactgcggt gtagcttcat tagccatggt ttttggctac tatggtagtt attatttttt
      481 ggctcacttg cgagaattgg ctaagacgac catggatggg acgacggctt tgggcttggt
      541 caaggtggca gaggagattg gttttgagac gcgagccatt aaggcggata tgacgctttt
      601 tgacttgccg gatttgactt ttccttttgt tgcccatgtg cttaaggaag ggaaattgct
      661 ccactactat gtggtgactg ggcaggataa ggatagcatt catattgccg atccagatcc
      721 cggggtgaag ttgactaaac tgccacgtga gcgttttgag gaagaatgga caggagtgac
      781 tctttttatg gcacctagtc cagactataa gcctcataag gaacaaaaaa atggtctgct
      841 ctcttttatc cctatattag tgaagcagcg tggcttgatt gccaatatcg ttttggcaac
      901 actcttggta accgtgatta acattgtggg ttcttattat ctgcagtcta tcattgatac
      961 ctatgtgcca gatcagatgc gttcgacact agggattatt tctattgggc tagtcatcgt
     1021 ctacatcctc cagcaaatct tgtcttacgc tcaggagtat ctcttgcttg ttttggggca
     1081 acgcttgtcg attgacgtga ttttgtccta tatcaagcat gtttttcacc tccctatgtc
     1141 cttctttgcg acacgcagga caggggagat cgtgtctcgt tttacagatg ctaacagtat
     1201 catcgatgcg ctggcttcga ccatcctttc gattttccta gatgtgtcaa cggttgtcat
     1261 tatttccctt gttctatttt cacaaaatac caatctcttt ttcatgactt tattggcgct
     1321 tcctatctac acagtgatta tctttgcctt tatgaagccg tttgaaaaga tgaatcggga
     1381 taccatggaa gccaatgcgg ttctgtcttc ttctatcatt gaggacatca acggtattga
     1441 gactatcaag tccttgacca gtgaaagtca gcgttaccaa aaaattgaca aggaatttgt
     1501 ggattatctg aagaaatcct ttacctatag tcgagcagag agtcagcaaa aggctctgaa
     1561 aaaggttgcc catctcttgc ttaatgtcgg cattctctgg atgggggctg ttctggtcat
     1621 ggatggcaag atgagtttgg ggcagttgat tacctataat accttgctgg tttactttac
     1681 caatcctttg gaaaatatca tcaatctgca aaccaagctt cagacagcgc aggttgccaa
     1741 taaccgtcta aatgaagtgt atctagtagc ttctgagttt gaggaaaaga aaacagttga
     1801 ggatttgagc ttgatgaagg gagatatgac cttcaagcag gttcattaca agtatggcta
     1861 tggtcgagac gtcttgtcgg atatcaattt aaccgttccc caagggtcta aggtggcttt
     1921 tgtggggatt tcagggtcag gtaagacgac tttggccaag atgatggtta atttttacga
     1981 cccaagtcaa ggggagatta gtctgggtag tgtcaatctc aatcagattg ataaaaaagc
     2041 cctgcgccag tacatcaact atctgcctca acagccctat gtctttaacg gaacgatttt
     2101 ggagaatctt cttttgggag ccaaggaggg gacgacacag gaagatatct tacgggcggt
     2161 cgaattggca gagattcgag aggatatcga gcgcatgcca ctgaattacc agacagaatt
     2221 gacttcggat ggggcaggaa tttcaggtgg tcaacgtcag agaatcgctt tggcgcgtgc
     2281 tctcttgaca gatgcgccgg tcttgatttt ggatgaggcg actagcagtt tggatatttt
     2341 gacagagaag cggattgtcg ataatctcat ggctttggac aagaccttga ttttcattgc
     2401 tcaccgcttg actattgctg agcggacaga gaaggtagtt gtcttggatc agggcaagat
     2461 tgtcgaagaa ggaaagcatg ctgatttgct tgcacagggt ggcttttacg cccatttggt
     2521 caatagctag aaagaggaga ggatgaaacc agaattttta gaaagtgcgg agttttataa
     2581 tcgtcgttac cataattttt ccagtagtgt gattgtaccc atggcccttc tgctcgtgtt
     2641 tttacttggc tttgcaactg ttgcagagaa ggagatgagt ttgtccacta gagctactgt
     2701 cgaacctagt cgtatccttg caaatatcca gtcaactagc aacaatcgta ttcttgtcaa
     2761 tcatttggaa gaaaataagc tggttaagaa gggggatctt ttggttcaat accaagaagg
     2821 ggcagagggt gtccaagcgg agtcctatgc cagtcagttg gacatgctaa aggatcaaaa
     2881 aaagcaattg gagtatctgc aaaagagcct gcaaggaggg gagaaccact ttccagagga
     2941 ggataagttt ggctaccaag ccacctttcg cgactacatc agtcaagcag gcagtcttag
     3001 ggctagtaca tcgcaacaaa atgagaccat cgcgtcccag aatgcagcag ctagccaaac
     3061 ccaagccgaa atcggcaacc tcatcagtca aacagaggct aaaattcgcg attaccagac
     3121 agctaagtca gctattgaaa caggtgcttc cttggccggt cagaatctag cctactctct
     3181 ttaccagtcc tacaagtctc agggtgagga aaatccccaa actaaggttc aggcagttgc
     3241 acaggttgaa gcacagattt ctcagttaga atctagtctt gctacttacc gtgtccagta
     3301 tgcaggttca ggtacccagc aagcctatgc gtcagggtta agcagtcaat tggaatccct
     3361 taaatcccaa cacttggcaa aggttggtca ggaattgacc cttctagccc agaaaatctt
     3421 ggaggcagag tcaggtaaga aggtacaggg aaatctttta gacaagggga aaattacggc
     3481 gagtgaggat ggggtgcttc atcttaatcc tgagaccagt gattctagca tggttgcaga
     3541 aggtacccta ctagcccaac tttatccatc tttggaaaga gaagggaaag ccaaactcac
     3601 agcttatcta agttcaaaag atgtagcaag aatcaaggtc ggtgattctg ttcgctatac
     3661 tacgactcat gatgccggga atcaactttt cctagattct actattacaa gtattgatgc
     3721 gacagctact aagactgaga aagggaattt ctttaaaatc gaggcggaga ctaatctaac
     3781 ttcggagcag gctgaaaaac ttaggtacgg ggtggaaggc cgcttgcaga tgattacggg
     3841 caagaaaagt tacctacgtt attatttaga tcaatttttg aacaaagagt aatgttcgtg
     3901 tttttagagt taaataattt ttaaactgtg agaaagattc ttcttgcagt tttttcttta
     3961 caatttttga aaaacatctg ccatttattc ggttaaattc ttgtgttttt tggttttttg
     4021 tggtaaaatg tgctcaagta atacgaaagg cgaactttaa aatgtcaaaa caattgatct
     4081 attcgggaaa agctaaagat atctatacaa ctgaggatga aaatcttatt atttcaactt
     4141 acaaggacca ggcgactgct ttcaacggtg tcaagaagga gcaggttgca ggtaagggag
     4201 tcttgaataa tcagatctca tcttttattt ttgagaaatt aaatgcggct ggtgtggcga
     4261 ctcactttgt ggagaaactt tcagacacgg aacaactcaa taaaaaagtt aagattattc
     4321 ctttggaagt cgtgctccgc aactatactg ctggttcctt ttcaaaacgt tttggtgtgg
     4381 atgagggaat cgccttggag actccgattg tcgaatttta ctacaaaaat gatgatttgg
     4441 atgatccatt tatcaatgat gaacatgtga aattcctaca gattgcggat gaccagcaga
     4501 ttgcctactt gaaggaagaa acgcgtcgta tcaatgaact attgaaagtc tggtttgctg
     4561 agattgggct taaattgatt gactttaagc tagagttcgg ttttgataag gatggcaaga
     4621 ttatcttggc agacgaattt tcaccagata actgccgctt gtgggatgct gatggcaacc
     4681 acatggataa ggatgttttc cgtagaggat tgggagaact aaccgacgtt tatgagattg
     4741 tttgggaaaa gttgcaggaa ttgaaataat ctgtttgcaa tggaaaacct tcgtctctca
     4801 actaaaagga ctcaggctga aaaggtcccc cagacctttt cactctgtag agaactaggt
     4861 gaactaaccg atgtttacga aattgtctgg gaaaagttgc agggtttaaa ataacaacct
     4921 caaggctgtt taggaatatt gcaagagctg aaataaagga ataagaattg atggataaac
     4981 gtatttttgt tgaaaaaaag gctgattttc aggttaagtc agagagtttg gttagagagt
     5041 tccagcacaa cttgggactg tcaagcttga aaagtattcg tattgtgcaa gtatatgatg
     5101 tatttgactt ggctgaggac ttgtttgcac ctgcagagaa gcacattttc tctgagcagg
     5161 taaccgacca tgttttagat gaagtatctg tgcaggcgga tcttgctaac tatgctttct
     5221 ttgccattga aagtctgcca gggcagtttg accagtgtgc agcttcgtca caggaagcct
     5281 tgcttttgtt gggaagttcg agtgacgtga cagtcaacac agcccaactt tacttggtga
     5341 ataaagatat tgatgcgact gagttggaag ctgtcaaaaa ctacctgctc aatccagttg
     5401 attctcgttt caaggatatc acgacaggga ttgccaagca ggagttttca gagtcagaca
     5461 agatcattcc aaaattgact ttctttgaaa gctatacagc agaagacttt gctcgctaca
     5521 aggccgaaca agggatggcc atggaagtgg atgatttgct ctttatccaa gactacttta
     5581 agtcaatcgg gcgcgtgcca actgagactg aactcaaggt tttggacact tactggtctg
     5641 accactgccg tcatacgact tttgagacag agttgaaaca catcgacttt tcagcttcta
     5701 aatttcaaaa gcaattgcag tcaacctatg acaagtatat tgccatgcgc gaggaattag
     5761 gtcggtctga aaaaccacaa accttgatgg atatggcgac tattttcggt cgttatgagc
     5821 gtgctaatgg acgattggat gatatggaag tctctgacga aatcaatgcc tgctcagttg
     5881 aaattgaagt ggacgttgat ggtgtcaagg aaccttggct tctcatgttt aaaaacgaaa
     5941 cccacaacca tccaacagaa attgagccat ttggtggagc ggctacctgt attggtggag
     6001 ctattcgtga tccgttgtca ggccgttcct atgtttacca agccatgcgt atttcaggtg
     6061 ctggtgatat tacagcaccg atttcggaaa ctcgcgctgg gaaattgcca caacaagtca
     6121 tttctaaaac agcagctcat ggttattctt catatggtaa ccagattggg cttgcaacaa
     6181 cctacgttcg tgaatacttc cacccaggct ttgtagctaa acgtatggaa cttggtgccg
     6241 ttgttggtgc ggctcccaag ggcaatgttg tccgtgaaaa acctgaagca ggtgatgtga
     6301 tcatccttct cggaggcaaa acaggtcgtg atggtgtcgg tggtgcgacg ggctcttcta
     6361 aggttcaaac agttgagtct gtagagactg ctggtgctga ggttcaaaaa ggaaatgcca
     6421 tcgaagaacg caagattcag cgcctcttcc gtaatggcaa tgtcactcgt ctgatcaaga
     6481 agtccaatga ctttggggca ggcggtgtct gtgtggctat cggtgaattg gcagacggtc
     6541 ttgaaatcga cctcaacaag gtgcctctta aataccaggg cttgaatggt acagaaattg
     6601 ccatctctga atcacaagaa cggatggcgg tcgtggttcg tcctgaagat gtggatacct
     6661 tcgttgccga atgtaacaaa gaaaatattg atgctgttgt ggtggcgaca gtaactgaaa
     6721 aaccaaatct tgtcatgcac tggaatggtg agacaatcgt tgacttggag cgtcgtttcc
     6781 ttgacaccaa tggtgtgcgc gtggttgtcg atgccaaagt tgtggacaag gatgtcaaac
     6841 tcccagaaga gcgtcaaaca tctgctgaaa cactggaatc agataccctt acggttctat
     6901 ctgacctcaa ccatgcaagt caaaaaggat tacagactat ctttgactgc tctgttggac
     6961 gctcaacggt taatcaccca cttggtggtc gttaccaact cacaccaact gaggcatctg
     7021 tgcagaaatt gccagttcaa cacggtgtga ctcatactgc gtcggtcatt gctcaaggtt
     7081 tcaacccata tgtagctgaa tggtctccat accacggtgc tgcttatgcg gttatcgaag
     7141 taactgctcg tttggtggct gctggtgcca actggtccaa ggctcgtttc tcttaccaag
     7201 agtatttcga gcgtatggat aaacaagcag agcgtttcgg tcagccagta gctgctcttc
     7261 taggttctat tgaagcacaa attcagcttg gcttgccatc tatcggtggt aaggactcca
     7321 tgtctggtac ctttgaagaa ttgaccgttc cgccaacctt ggttgccttt ggggtgacga
     7381 cggcagatag ccgtaaggtg ctctctccag aatttaaagc tgttggggaa aatatctact
     7441 acatcccagg tcaagccctc tctgcagaga ttgattttga cttgattaag aaaaattttg
     7501 ctcagtttga agccatccaa gctgaccata aagtgacatc tgcatcagct gtcaaatacg
     7561 gtggtgtagt tgaaagtttg gctcttgcta cctttggaaa ctatattggt gcagaggtga
     7621 ccttgcctga acttaaaaca gctttgacag ctcaattagg cggctttgtc ttcacatctc
     7681 ctgaagaaat tgctggagta gagaaggttg gacaaacgaa agcagacttt acactgactg
     7741 tcaacggtgt gaagctagat ggacacaagc ttgacagtgc atttcaaggg acattggaag
     7801 aagtttaccc aacagaattt acccaagcga aagaactaga agaagtacca gctgtggcat
     7861 cagatgttgt gattaaagcc aaagaaaagg ttgaaaaacc tgtggtttac atcccagtct
     7921 ttccaggaac caactcagaa tatgattcag ctaaggcctt cgaaaaagaa ggtgcagagg
     7981 tcaatttggt gccattcgtg accttgaatg aagaagctat tgtcaagtca gttgaaacta
     8041 tggttgacaa tatcgacaag actaatattc tcttctttgc tggtggattc tcggctgcgg
     8101 atgaaccaga tggttcagct aagtttatcg tcaatatcct gcttaatgaa aaagtgcgtg
     8161 tggctattga tagctttatc gcccgtggtg gtttgattat cggtatttgt aatggattcc
     8221 aagccttagt caaatcgggt ctcctaccat acggaaactt tgaagctgct aacagtacta
     8281 gcccaaccct cttctacaat gatgccaacc aacacgtggc caagatggtg gaaactcgca
     8341 ttgccaatac caactcacca tggttggctg gtgtgcaagt gggcgatatc cacgctattc
     8401 ctgtttcgca cggtgaaggg aagtttgtcg tgacggctga ggaatttgca gagctccgtg
     8461 acaatggaca aattttcagc caatacgttg actttaacgg taaaccaagt atggattcta
     8521 agtacaatcc gaatggttct gtccatgcca tcgaaggaat taccagcaag aatggtcaaa
     8581 tcatcggtaa gatgggccac tcagaacgtt atgaggatgg tcttttccaa aatatcccag
     8641 gcaataaaga ccaacacctg ttcgcatcag cggttaaaca tttcactgga aaataagact
     8701 tacagatttt ctaatagata gtatcagtaa tgtaaaagtc atgtaaatct agctcttgat
     8761 gattacaaat gaaaattagg tataaaaaat gacatacgaa gtaaaatctc ttaatgaaga
     8821 atgtggtgtt ttcggtattt ggggacatcc agatgctgct aagttgacct attttggact
     8881 ccacagtctt caacaccgtg gtcaggaggg ggcaggaatc ctctccaacg atcaaggaca
     8941 actgaagcgc catcgtgaca tggggctttt atcagaagtt ttcagaaatc cagctaattt
     9001 ggataaattg acaggagctg gtgcgattgg gcatgtgcgt tatgcgactg ctggcgaagc
     9061 ttctgtagat aacatccagc ccttcctctt ccgttttcac gatatgcagt ttggcttggc
     9121 tcataatgga aatctgacca atgcagcctc tctcaagaaa gaactggaac aaagaggagc
     9181 aattttcagc gcgacttcgg actctgaaat cttggctcac ctcattcgtc gcagtcataa
     9241 tcctagcctg atgggcaaaa tcaaggaagc gctcagcctt gtcaaaggtg gttttgccta
     9301 tatcttgctg tttgaggaca agttgattgc ggctcttgac cctaatggct ttcgtccgct
     9361 ttctatcggg aaaatggcca acggagcggt ggttgtttcc tctgaaacct gtgcttttga
     9421 ggtcattggt gccgagtgga ttcgtgattt gaagccaggt gagattgtga tcattgatga
     9481 cgagggcatt cagtatgaca gctatacaga tgatacccag ttggcgattt gttctatgga
     9541 gtatatctac tttgcccgcc ctgattctaa tatccacggt gtcaatgtcc atacggcacg
     9601 taagagaatg ggagcgcaat tggcgcgaga atttaagcat gaggcagata ttgtagttgg
     9661 tgtgcccaat tcttccctaa gcgcggctat gggatttgcg gaagaatcag gcttaccaaa
     9721 tgaaatgggt ctgatcaaaa accaatacac ccagcgaact tttatccaac cgactcaaga
     9781 attgcgggag caaggagtgc ggatgaaact gtctgctgtt tcgggtgttg tcaaaggcaa
     9841 acgtgtggtc atggtggatg attccattgt acgtggaaca acctctcgtc gtatcgttca
     9901 gctcttgaaa gaagcgggtg cgactgaggt tcacgttgcc attggaagtc ctgcactagc
     9961 gtatccatgt ttctacggga ttgatatcca gacccgtcag gagctgattg cagccaatca
    10021 tacggtcgaa gaaacttgcc aaatcattgg tgcggacagt ctgacttatc tttcaattga
    10081 tagcttgatt gagtcgattg gtatcgaaac agatgcgccg aacggtggtc tctgtgtcgc
    10141 ttactttgac ggtgactacc caacgcctct ctatgactac gaagaagact atcgtagaag
    10201 tttggaagaa aagaccagtt tttacaagta ggcgacagat tctccattaa agaaaaggaa
    10261 aaaacaaatg acaaataaaa atgcatatgc ccagtcgggt gtggatgttg aagcgggtta
    10321 tgaagttgtt gaacggatta aaaagcacgt ggcccgtacg gagcgtgcag gtgtcatggg
    10381 agctcttggt ggctttggtg gtatgtttga cctttccaag actggggtta aagaacccgt
    10441 cttgatttca gggactgacg gtgtcggaac caagctcatg ttggctatca agtacgacaa
    10501 gcacgatacc atcgggcagg actgtgtggc catgtgtgtc aacgacatca ttgctgcagg
    10561 tgcggaaccc ctctattttc tcgactacgt agcgacaggg aagaatgagc cagctaagct
    10621 agaacaagtg gttgctggtg tggcagaagg ttgtgtgcag gctggtgctg ccctcatcgg
    10681 tggggaaacg gctgaaatgc cgggcatgta cggcgaagac gactatgact tggctggttt
    10741 tgcggtcggt gtggctgaaa aatctcaaat cattgacggt tcaaaggtgg tagagggaga
    10801 cgttcttctc ggacttgctt caagtgggct tcactcaaat ggttactctt tggttcgtcg
    10861 tgtctttgcg gattacacag gtgaggaagt cctaccagaa ttggaaggca agaaacttaa
    10921 ggaagttcta cttgagccga ctcgtatcta tgtcaaggct gtcttgccgc tcatcaaaga
    10981 agagttggtc aacggcattg cccacatcac aggtggtggc tttatcgaaa atgtccctcg
    11041 tatgtttgca gatgacctag ctgctgaaat tgatgaaagc aaagttccag tgcttccaat
    11101 tttcaaagcc cttgaaaaat atggtcagat taaacacgaa gaaatgtttg aaatcttcaa
    11161 tatgggtgtg ggacttatgt tggcggtcag ccctgaaaat gtagagcgtg taaaagaatt
    11221 gttggatgaa gcagtctatg aaattggtcg catcgtcaag aaagaaaacg aaagtgtcat
    11281 tatcaaatga aaaaaatagc ggtttttgcc tctggtaatg gctcaaattt tcaggtgatt
    11341 gccgaagaat ttccagtgga gtttgtcttt tcagaccatc gtgatgccta tgtgcttgag
    11401 cgtgcaaagc agctcggcgt tctgtcctat gcttttgaac tcaaggagtt tgagagcaag
    11461 gcagactacg aagcagccct tgtcgaactc ttggaagaac accagattga cttggtttgc
    11521 ctagcaggct acatgaaaat cgttggacca accttattgt cggcttatga aggtcggatt
    11581 gtcaacattc atccagccta cttgccagaa tttccaggag ctcatgggat tgaggatgct
    11641 tggaatgctg gcgtgggtca gtctggtgtg accattcact gggtggattc gggtgtggat
    11701 acaggccagg tcatcaaaca ggttcgtgtg ccacgactag ctgatgatac cattgacaga
    11761 tttgaagctc gcatccatga agcagagtac aggttgtatc cggaagtagt gaaggctcta
    11821 tttacagatt gactttttga tgattcatat gatatctttg attttaaatt ggagtcagtg
    11881 tttgttgaag acggcttcaa acggaggtat ttgtaatgtt agaatctaaa aaaacaactc
    11941 gatatgtatt ttatgtctat ctgatgttat taacttgggg aatcttattt aagtttgaaa
    12001 caaatcctga atttatagca tttttcttag ctccaaggta tatcaattgg attccatttt
    12061 cagaaccact aatagtcgat ggaaaaattg tttttgctga aatattattt aatctgattt
    12121 cctttattcc attaggtgtt tgtttccctt tgataaaaac taatttatct agtttaagaa
    12181 tagtcgggac aggtttcttg attagtttat tgtttgagtg cttacagtat attttagcaa
    12241 taggtataac agatataacg gatttgactt taaatacgct aggtgtctgt gtaggcttac
    12301 tgatttatca aatttttata agagtgttca aatcacagac tagaaaatgg atcaatatct
    12361 taggtatgct tagccttggt tttgcttatc ttgttttact gttactgcat ttaattggtg
    12421 tttaactaat gattaaaaag gagaatataa tgactaaacg cgtcttaatc agcgtctcag
    12481 acaaagcggg cattgttgaa tttgcccaag aactcaaaaa acttggttgg gagattatct
    12541 caacaggtgg aactaaggtt gcccttgata atgctggggt ggataccatt gctatcgatg
    12601 atgtgactgg tttcccagaa atgatggacg gtcgtgtgaa gaccctccac ccaaatatcc
    12661 acggagggct tctcgctcgt cgtgacttgg atagccactt ggaagcggct aaggacaaca
    12721 agattgagct cattgacctt gtggtggtca acctttaccc atttaaggaa actatcctta
    12781 aaccagatgt gacttatgct gatgcagttg aaaatatcga tattggtggg ccgtctatgc
    12841 ttcgttcagc agcgaaaaat catgccagtg ttacagttgt ggtagatcct gctgactacg
    12901 ctgtggtttt ggacgaattg gcagcaaacg gcgaaacctc ttatgaaact cgccaacgtt
    12961 tagcagccaa agtatttcgt cacacagcgg cttatgacgc cttgattgca gaatacttca
    13021 cagctcaagt gggtgaaagc aagcctgaaa aactcacttt gacttatgac ctcaagcaac
    13081 caatgcgtta cggtgagaat cctcaacaag acgcggactt ttaccagaaa gctttgccta
    13141 cagattactc tattgcttca gccaaacagc tcaacgggaa agaattgtca tttaataata
    13201 tccgtgatgc agatgctgct atccgtatca tccgtgactt caaagatagt ccaaccgttg
    13261 tggctctcaa acacatgaat ccatgtggaa ttggtcaagc tgatgacatc gagactgctt
    13321 gggactacgc ttatgagtct gacccagtat ctatctttgg tgggattgtc gtcctcaacc
    13381 gtgaggtgga tgctgcgaca gctgagaaga tgcacggcgt tttcctcgaa atcatcattg
    13441 caccaagcta tacggatgaa gcgctagcca ttttgatcaa taaaaagaaa aacttgcgta
    13501 tccttgcctt gccatttaat gctcaagagg ctagcgaagt ggaagcagaa tacacaggtg
    13561 tagtcggtgg acttctcgtg caaaatcaag acgtggtcaa ggaaagccca gctgactggc
    13621 aagtggtgac taaacgtcag ccaactgaga cagaagcgac tgctcttgag ttcgcttgga
    13681 aggctatcaa gtacgtcaaa tcaaatggta ttatcgtgac caacgaccac atgacacttg
    13741 gtgttggtcc aggtcaaacc aaccgtgtgg cttctgttcg ccttgccatt gaccaagcca
    13801 aagatcgtct ggacggggcg gtccttgctt cagatgcctt cttcccattt gcggataacg
    13861 tggaagaaat cgccaaagca ggaattaagg ccatcatcca gcccggtggc tctgtccgtg
    13921 accaagaatc catcgaagca gcggataaat acggcttgac tatggtcttt acaggtgtga
    13981 gacattttag acattaagaa gataaaaggg aagaaaacag tttctttcct tttttggctt
    14041 aaaatgctaa ctgaaacaag attaaaacga acttttttga tataatgttg gtaaataatt
    14101 cgcaaaagag gttgaggaat gaaactgctt gttgtcggtt ctggtggtcg tgagcatgcg
    14161 attgctaaaa agttacttga atcaaaagac gtggaaaaag tctttgtagc tcctgggaat
    14221 gatgggatga ctctggatgg tttggaattg gtaaatatct ctatttccga acattctaaa
    14281 ttgattgact tcgcaaagac caatgatgtt gcttggacct ttatcggtcc agatgatgcc
    14341 cttgctgctg gcatcgtgga tgattttaac caagctggac ttaaggcctt tggtccgact
    14401 agggctgcag cggagctgga gtggtccaag gatttcgcca aggaaatcat ggtcaaatac
    14461 ggcgttccga cagcaatata tggcacattt tcagatttcg aggaagccaa ggcctatatc
    14521 gaaaagcatg gtgcgcctat cgtagtcaag gcggatggct tggcacttgg gaagggtgtc
    14581 gtcgttgctg agacggttga gcaagcggtc gaagccgctc atgagatgct tttggacaat
    14641 aaatttggtg actcaggtgc gcgcgtggtt attgaggaat tccttgaagg agaggaattt
    14701 tcactctttg cctttgtcaa tggtgataag ttctacatca tgccaacggc tcaggaccac
    14761 aaacgtgcct atgatggcga caaagggcct aacacgggtg gtatgggtgc ctatgcgcca
    14821 gttccacact taccacagag tgtagttgat acagcggttg acaccattgt caagccagtt
    14881 ctagaagggg tgattaaaga aggtcgtcct tatctgggag ttctttacgc agggcttatc
    14941 ctgacagctg atggaccgaa agtcattgag ttcaacgctc ggttcggaga tccagaaact
    15001 cagattatct tgcctcgctt gacctctgac tttgctcaaa atatcacaga tatcctggat
    15061 agcaaggagc caaatatcat gtggacggac aagggtgtga ctctgggtgt ggttgtcgca
    15121 tccaagggct acccgctaga ctatgaaagg ggcgttgagt tgccagccaa gacagaaggc
    15181 gatgtcatca cctactatgc aggggctaag tttgcggaaa atagcagagc actgctctca
    15241 aacggcggac gagtttatat gctcgttacc acagcagata ccgtcaaaga agcccaagcc
    15301 agcatctacc aagaactatc ccaacaaaaa atagaaggac tcttctaccg aacagatatc
    15361 ggaagcaagg caattaagta aagatataag aataacgcgc cgtagtcgcc aaacacgata
    15421 atggtcgtcg tggtgaaaag accagaacag tgaatgttct ggtcaggggg aaacttggag
    15481 accttaggct caaagtttag gaatgaaacc gaaggtttgc ttccgcctcc atcacctaag
    15541 accattatca aaaagaaaaa taaaaattca caaaatacgt taatgatcgt atggtttgcg
    15601 agcgttagcg agctaatata gaacaatcac cgccgttgtg aaagaacgat tggatgataa
    15661 tccaatcgtt cagggaaatt ggaagacctt gggtttccaa tttaggcatg agacaccttt
    15721 ggtggctgct gccgtccctc acaagctaag gtgattgttg aaaaagagga aaaaggagaa
    15781 gaaatgaaac cagtaatttc catcatcatg ggctcaaaat ccgactgggc aaccatgcaa
    15841 aaaacagcag aagtcctaga ccgcttcggt gtagcctacg aaaagaaagt tgtttccgca
    15901 caccgtacac cagacctcat gttcaaacat gcagaagaag cccgtagtcg tggcatcaag
    15961 atcatcatcg caggtgctgg tggcgcagcg catttgccag gcatggtagc tgccaaaaca
    16021 acccttccag tcattggtgt gccagtcaag tctcgtgctc ttagtggagt ggattcactc
    16081 tattctatcg ttcagatgcc gggtggggtg cctgttgcga ccatggctat cggtgaagct
    16141 ggagcgacta acgcagctct ctttgccctc cgtctcctct ctgtagaaga taagtccatt
    16201 gcggatgcac ttgccaactt tgctgaagaa caaggaaaaa tcgcagagga gtcgtcaaat
    16261 gagctcatct aaaacaatcg gaattatcgg tggcggtcaa ctgggtcaga tgatggccac
    16321 ttctgctatc tacatgggcc acaaggttat cgcgctggat cctgcggcgg attgcccggc
    16381 ctctcgcgtg gcggaaatca ttgtggcacc ttataacgat gtggacgccc tccgtcagtt
    16441 ggcagaccgt tgcgatgtcc tcacttacga gtttgaaaat gtcgacgctg acggtttgga
    16501 tgccgttatc aaggatggac aactccctca aggaacagat ctgctccgca tttcgcaaaa
    16561 tcgtattttt gaaaaggact ttttgtcaaa caaggctcaa gtcactgtgg caccctacaa
    16621 ggtcgtgact tctagcctag acttggcaga tatcgacttg tcgaaaaact atgtcctcaa
    16681 gactgcgact ggtggctacg atggtcatgg acaaaaggtt attcgttcag aagcagactt
    16741 ggaagcagcc tatgcgctag cagactcagc agactgcgtc ttggaagaat ttgtcaactt
    16801 tgaccttgag atttctgtca tcgtgtcagg aaatggcaag gaggtgacgt ttttcccagt
    16861 tcaggaaaat atccaccgca acaatatcct gtctaagacc atcgtaccag cccgcatttc
    16921 tgaaagtcta gcagacaagg ctaaagctat ggcagtgcga atcgcagaac aactcaactt
    16981 gtctggaact ctctgtgtgg aaatgtttgc gactgatgat gacgttattg tcaatgaaat
    17041 cgccccacga ccacataact ctgggcacta ttctattgaa gcctgtgatt tctctcagtt
    17101 tgacacccat attctgggtg ttctcggagc accattacca gtcatcaaac tccatgcgcc
    17161 agccgttatg cttaatgtcc tcggtcagca tgtcgaggct gctgagcaat atgtgacaga
    17221 aaattcaagc gcccacctcc acatgtatgg taaaatagaa gcgaagcaca accgcaagat
    17281 gggacatgtg accttgttta gtgatgtacc ggatagtgtg gatgagcttg gggaagggat
    17341 tgatttttag gacaagtcta tgatacaaat tatcgttaat acatttattg aaaaggataa
    17401 gactggagca gttgttgaag tgttgtatgc cagtgctgac caagataagg tacaagctaa
    17461 atatgaagaa ctagctgcac aataccccga aaattattta gctatctatg atgtaccgct
    17521 ggatacggat ttgaatacac tagatcatta cccgtctgtg tttattgaaa aagaggagtt
    17581 tgagtagaaa tcttggttta cctagatagc ttattcccaa cagcttaaga agaaaggaaa
    17641 aattaacaca tgatcaaccg ttactctcgc cctgagatgg cgaatatttg gagtgaagaa
    17701 aataaatacc gtgcttggct tgaggtggaa atcctctctg acgaggcatg ggctgagttg
    17761 ggggaaatcc ctaaggaaga tgtggctttg attcgcaaga aggcggactt tgacatcgac
    17821 cgtattttgg aaattgagca ggagacgcgc cacgatgtgg tggctttcac gcgtgcggtt
    17881 tctgagactc ttggtgaaga gcgcaagtgg gttcactatg ggttaacttc taccgacgtg
    17941 gtggatactg cttatggtta cctctacaag caggccaacg acatcatccg tcgtgacctt
    18001 gaaaacttca ctaatatcat cgctgacaag gccaaggagc acaagttcac catcatgatg
    18061 gggcgtactc atggtgtgca cgctgagccg acaacctttg gtcttaaatt ggcaacttgg
    18121 tacagcgaaa tgaaacgcaa tatcgagcgt ttcgagcatg cagctgctgg tgtagaagct
    18181 ggtaagattt ctggtgcggt tgggaacttt gccaatatcc caccatttgt agaggagtat
    18241 gtctgcgata aacttggcat ccgtgcccaa gaaatctcta cacaagtcct tcctcgtgac
    18301 cttcacgctg agtactttgc ggttcttgcc agcattgcga cttcaatcga acgtatggcg
    18361 actgagattc gtggtctaca aaaatctgag caacgcgaag tagaagagtt ctttgctaaa
    18421 gggcaaaaag ggtcttcagc aatgcctcac aaacgcaacc caatcggttc tgaaaatatg
    18481 actggtctgg cgcgtgtcat tcgtggtcac atgattacgg cttatgaaaa cgtcgctctc
    18541 tggcacgaac gcgatatttc tcactcatca gctgagcgta tcatcacacc agatacgacc
    18601 attttgattg actacatgct caaccgtttt ggaaatatcg tcaagaactt gacagtcttc
    18661 ccagaaaata tgatccgaaa catgaactcg acttttggtc ttatctttag ccaacgggct
    18721 atgttgacat tgattgaaaa aggcatgacc cgtgagcaag cctatgactt ggtgcaacca
    18781 aaaacagcct actcttggga caaccaagta gactttaaac cacttcttga ggcagattca
    18841 gaagtaacat cacgtctcac tcaagaagaa atcgatgaaa tcttcaaccc agtttattac
    18901 accaaacgag tggatgatat ctttgaacgt cttggactag gtgattaatt aaaaaataaa
    18961 cagcgagctt caatctcgct gtttattttt tatcgaaaag acttagtctt cttttctttt
    19021 agtgagtcca taggctgcta gtgtggccat gagtcctgcg actactagtc ctgcagaatc
    19081 gtgagttcct gtttcaggaa gttttttctc tattaccaca ggagctggat cttgaggaag
    19141 agctttgctt tcctcagcag gagcagttga tggagctggt tggcttggga tttctagttt
    19201 tggtttttct tcagcaatag cggcttgtcc gttttcatcg cctacatgtg ttaccatagt
    19261 tccgacttcg actatttgag taacggcttc ctgtgctacg acactattta caagtgtttt
    19321 cacttcctta ccatcggcag aagtgctcac agagtagaag ttgctacgat gtccattgac
    19381 gcccttagta atgacttgtg tttttccttt gagtaagagt ggattttcac gagtcactgt
    19441 ggtaaatgga atttcttctt tttggatatc cagtctaggt tttacctcag tagttggtgc
    19501 aagaccactt tcatcaccct tgtgagttac aggagcgcca acttcaacca cttggtttat
    19561 aacttctttg gttacctggc tatcaaggac tgtttctgtt gtttttccat tttcagtgag
    19621 tacagagatg taatgagttc gctcaccttt gactcctgct gtgataatat tttcctgacc
    19681 agctgggagg ttaggatttt ctttcttgat aacttcaaat ggaatttctt cagttcttgt
    19741 gatgagttct ggtctggttt caacattggc agccacttca ttttcatcca ggcttcctga
    19801 atgagttgca gctggtttga ggccttgaag agcggctttt aggttggcta caagtgtgtc
    19861 aagctcagct tgtttattac ggttgaggtt gtaatttaga gctgttttag ctgcgtcaag
    19921 ggcctcaaga ctttctttac tatatccttc taagtttgta ggaattttag ctaattcttc
    19981 gcggagagca ttataattag cacggaagta gtctttgttg tggtctgcaa aggcagtcat
    20041 gagttcaaag atttcctctt ccttgtattc agcgcttggt ctatctgccc agattgaaag
    20101 catacttccg actgttggaa gatctacttc aggatatttg gtagaagcta gttgattgaa
    20161 tggtgttttt ccagtattct caatagcttt cttgaggaaa ccaccaccat cttctggttt
    20221 ttgaccaaga atgtagtacc agtcaccgtt ggtattcaag aatttatagc ctttgcttgc
    20281 taggtattga ggtgatgcga ggttatatcc ccaccagcct ttagaccagt aagaaatcaa
    20341 gacatctttg tcaaactgaa catcgtcctt gtcttcatag tagaagccat cgttgaaggc
    20401 cattggttga agccctcttt ctttggccat agctgcgagg gtgttggcat attcggcaaa
    20461 tttgccatag agttgatacc acttgaggta gtaccagcct tgggcactag tcgcatcgtt
    20521 ggcgtattcg tcagtaccaa agttgaaaat ctttgtttta cctgcaaaga agtccatgta
    20581 tttaccgatg agggctttta caaagttcat cgcttcttcg tttttcaagt ccatagttgt
    20641 ttttgaaact ttatcaaagt gggcttgagg atttttaata cctaattttt ccatggcaac
    20701 cagcatagca tccatgtgac ctggactgtt aatagctggg atgagaccga tgtccttaga
    20761 tttagcgtat tcaattagct ctgttacttc tgcctgtgtt agtgcagtac cgtttggatc
    20821 gtcgtagtaa gctttagttc cttcgataat agctttttta acgtcatcac tagcataggt
    20881 ttttccgttg gcagtaatgg tcatatcatc gagtagaaag cgaagtccgt catttcctag
    20941 aaggagatgg acatcagaat atccgagctc actggccttg tctacgatgc gtttgagctg
    21001 gttcagagta aagtatttgc gtccagcatc gattgagatt accttgtttt tggcaagttt
    21061 ttcaacctca cgtttagctt cttcttcttt ttgagcttca ggcgtgaggg tcaagttgtt
    21121 gacagtttct tgaagtttag caatggcttg atcaatcgta tcttgttggg cacggctaag
    21181 gttgctatcg agagagcgaa tagctttttc agcttctttt acggccgcga cgctttctgc
    21241 agtataacgg ttcaggtctt ttggtacctc gttaagtgtt tgctctgcag attcataatc
    21301 agctgcgaag tattcagcgt tggcatttgc aaaatgacgc atgagtttga agaggcgtga
    21361 tggtgaataa cgtgcagatg gagtgtcagc ccaagcagct accataccac cgatgattgg
    21421 gatatcagct ccttctgttt ttggtacaga agtgattggt gtgtttttaa taccattgag
    21481 cccctgatcg agattgtacc agccttggcc atcagcgttt cgtccaagaa cgtagtacca
    21541 agcatcattg gtattaagga tttggtgacc tttttcagct agtagtttag aagaagcgac
    21601 atcgtagcct ccccaaccac cagtccacat agaaacgatg atgtctttgt caaaactacc
    21661 aaagcttgtg tcgctattgt agtagatacc gtcgttaaag gccattggtt tgagaccgtg
    21721 cgattttaca atacgagcga ggtcattggc gtaggcaata aatttttcat agccttttac
    21781 agggtagcct tcgtttggat agtatttatc agcttgaagc acactccaac ctttagcatc
    21841 tgtcgcatca ttggcatatt catcaagtcc gatgttgaag atttcagtct ttttcgcgaa
    21901 ataagcagca tacttgtcga taagggcttt tgtaaaagcg acagcttgtt cgttgtcaag
    21961 atcgacagta cgggctgatt tcttcccaaa atagctaaag ttagggtttt ggattcccaa
    22021 ttctttcatg gcattgagaa tcgcatccat gtgtccagga ctatttactg tcggaatgag
    22081 accgatacct ttatctttgg catagttaat caaatctgtc atttgacttt ctgttaagtg
    22141 attgccgttt ggatcgttgt aataatcatt tgtacctttt tcaatggcgc gtttgacatc
    22201 gtcactggca taggtcttgc cgttagctgt gatgctcata tcgtccaaca tgaaacggag
    22261 tccatcattt ccgactaata ggtgtaaatc agtgtagcca taatgtttcg ctttatcgat
    22321 gatttccttg agctgttctg gtgagaaata tttacgtcca gcatcaatag aaacaatttt
    22381 ctttttcgct agtttttcat ttacagttgc agcacgttcc tttcctgtct ctgtttccgg
    22441 tttgtcagcc tctgctttcg cttcatcttt tttagctggt ttatccttgt cagtcttgtc
    22501 tgtatttgac tctttagaat caacctcttt cgcttcttcc tttttagggc tagcttcttc
    22561 ttccttttta ttagcagttt ctttttcagc agaagttgga gttaccacct ctgctttatc
    22621 actaggagtt gaactaactt cctcttgtgg tttttcttct gtttttggaa gactagctac
    22681 cttatcagta gctggagttt ctgtttctac agtttttgga gcttctggtt gaagctctgc
    22741 tttaggtgtt tcctcagtcc gattttcgga tgattgaggg gaatcagaaa ctgtatggat
    22801 ggtcggttgg ttttctgtag tagtagtagt aactccatcg gctgcaacag tctgtgcttg
    22861 gaaggcaaat ccaattagaa cagaagctgc tcctacagcg tatttacgaa tagaaaaacg
    22921 ctgttgtttt tcatgtttca ttgcaaaacc tcctgattgc attgttatat tgatagcgat
    22981 tatataaatc aacgccctta ttttatttct tatattaacg agagtcaaga ggagatgaca
    23041 aaaaactata ataagtataa aaaaatataa aatttaaact taagatttca gattggtcgg
    23101 aaaaaatgca tatatatatc taatataatt tttggttcta tttctataaa atattccaca
    23161 aattatagaa tcttccaaaa ataggtaagc gctacctttt tggtgtggta taataagcat
    23221 agaaaaagcc cgagcgatta ggctcaggct ttcttcttag tgatcacggt cacatgagat
    23281 gaatttaatc ttgtagtaat cagatcgttt gtaagtttca ctgtattcta aaacttggcc
    23341 agttgattcg agtttggtga ttttagtttg taggacagta gggaaatgtt catcgactcc
    23401 gaggactgaa gccgcatgtt ctggagttgg aaagactatt tcgttgattt cttcaaagtg
    23461 ttcatcattc atgtgaatgt ggtagtctaa cttgaaacga ttatagatag aactatagta
    23521 ttcaaggttt ggataatttg cgttgatata ttgttctggg atgtaggatg tatggtagat
    23581 ataaacgaca ccgtttgatt cgcggatacg ttcaatcttg tagtagaatt gatcgccacg
    23641 tagacccaat ttttccaagt aaacaagctt gtttccgcgt tcaattgaaa gaacagttac
    23701 cttatcatct ttagcattga agagttcaat atctgaaaac tctacaagct tgtgtttgcg
    23761 tgcacgtgaa atgaaagttc cttttccttg ttggcggaca atatagccat ctttggcaag
    23821 gtcgtttaag gcgcgaacaa ctgtgataga gctgacatcg tacattgaaa tgagttctgc
    23881 ttcagtgtaa aatttatctc cactgctaaa ctgcccagag atgattttat tttttaattc
    23941 gtcttttatg tattgatatt tgggaattgc catattttta cctctatttt ccttctccat
    24001 agaaaattt
//
LOCUS       DAVGJL010000017        23352 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.017, whole genome shotgun sequence.
ACCESSION   DAVGJL010000017 DAVGJL010000000
VERSION     DAVGJL010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23352
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.017"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            10..288
                     /locus_tag="VVE42_000583"
     CDS             10..288
                     /locus_tag="VVE42_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3072830.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            290..2353
                     /locus_tag="VVE42_000584"
     CDS             290..2353
                     /locus_tag="VVE42_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU3072831.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIESIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRSFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTATDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRIRVTD
                     NQMNSRILQLIELMKKSEENKKTKQRYFIKMADKVVKYNFLGAGLTYLLTGSFSKAIS
                     FLLVDFSCALKISTPVAYLTVIKEGLNREMVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYSEEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLVRTKELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWLNPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(2431..3687)
                     /locus_tag="VVE42_000585"
     CDS             complement(2431..3687)
                     /locus_tag="VVE42_000585"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEU3072832.1"
                     /translation="MHIFDELKERGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADAVRFLKIFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADEHNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQDLDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            3829..6291
                     /locus_tag="VVE42_000586"
     CDS             3829..6291
                     /locus_tag="VVE42_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEU3072833.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     AIFPAILLSIKALFNLLFVLGFLGGMLGAGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEI
                     LTTYLNVAPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVTGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLREKGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPIQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNPTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALDPSVVKSEVLKSTGQKPGKVS
                     VEGKEVEVTGSTVTSYWANKAGAPATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSRARR"
     gene            6356..7267
                     /locus_tag="VVE42_000587"
     CDS             6356..7267
                     /locus_tag="VVE42_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU3072834.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCVEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVSVISASEPLSSALLSVLFLGLVV
                     DWSFLLAIALIILPMIFLSIEEAKESR"
     gene            7497..8195
                     /gene="dapD"
                     /locus_tag="VVE42_000588"
     CDS             7497..8195
                     /gene="dapD"
                     /locus_tag="VVE42_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEU3072835.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEI
                     GDNVVIMMGAVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVRVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            8263..9393
                     /locus_tag="VVE42_000589"
     CDS             8263..9393
                     /locus_tag="VVE42_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HEU3072836.1"
                     /translation="MLDLIQTRRDLHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLPFASQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEAGAFGDWLPDQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVN
                     PIEGAVVTFGVFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFD
                     MEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            9405..9944
                     /locus_tag="VVE42_000590"
     CDS             9405..9944
                     /locus_tag="VVE42_000590"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEU3072837.1"
                     /translation="MKSELRKQVLQGMKAISQEQKQFIDQTLTERLLHHPFYQEAKVI
                     ATYLSFPHEFQTQELIEQALKDGKKVLIPKTYPKGRMDFVVYDPQQLVKTSFGLLEPQ
                     GDLEVVDASQIDLIHVPGLAFTTKGYRIGYGGGYYDRYLEHFSGHTLSTVHPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            9928..10605
                     /locus_tag="VVE42_000591"
     CDS             9928..10605
                     /locus_tag="VVE42_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEU3072838.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQVWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIILRYATRNPYIQQLGQSYLTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRMYNTSQRLGAVVLFVGLAILL
                     FYKGMGM"
     gene            complement(10677..11576)
                     /gene="galU"
                     /locus_tag="VVE42_000592"
     CDS             complement(10677..11576)
                     /gene="galU"
                     /locus_tag="VVE42_000592"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEU3072839.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKTDLLKLVDKTTDMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKAVPLTKQLMDDYERTHAST
                     IAVMPVPHDEVSAYGVIAPQGEGKDGLYSVETFVEKPAPEDAPSDLAIIGRYLLTPEI
                     FQILENQAPGAGNEIQLTDAIDTLNKTQRVFAREFNGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            complement(11598..12614)
                     /locus_tag="VVE42_000593"
     CDS             complement(11598..12614)
                     /locus_tag="VVE42_000593"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEU3072840.1"
                     /translation="MEKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     HHTNKHYFKDVVLDENIIAYTDLAETLKDVDAILFVVPTKVTRLVAQQVAQTLDHKVI
                     IMHASKGLEPDSHKRLSTILEEEIPEHLRSDIVVVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQKLFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHGTNIKDAIYDIMNNEFKAE
                     NEWS"
     gene            12826..13206
                     /locus_tag="VVE42_000594"
     CDS             12826..13206
                     /locus_tag="VVE42_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU3072841.1"
                     /translation="MREFGEKIKRLRLAKKISRSEFCGDESELSIRQLIRIENGESRP
                     TLTKLKYIAERLGVEDYKLMPSYIELDKEYLELKYFLMRTPTYEDETIAQKKESVLIR
                     FLKSIMIGYLRKKDLSSQIIHIWH"
     gene            complement(13380..13805)
                     /locus_tag="VVE42_000595"
     CDS             complement(13380..13805)
                     /locus_tag="VVE42_000595"
                     /inference="COORDINATES: protein motif:HMM:NF025080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU3072842.1"
                     /translation="MTNLSSVDSEELFQFYRERGNAENFIKERKAGFFGDKTDSSTMI
                     KNEVRMMMGCLAYNLYLFLKQLAGDEVKALTIKRFRRLFLHIAGKYVSTARRHILKFS
                     SLYAYSKQFQALFDTICQINLILPVPYRARGQGKTCLTE"
     gene            complement(13873..14523)
                     /gene="phoU"
                     /locus_tag="VVE42_000596"
     CDS             complement(13873..14523)
                     /gene="phoU"
                     /locus_tag="VVE42_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEU3072843.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKALLALASKDKEMAEL
                     IINKDHAINQGQSAIELTCARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLADLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            complement(14538..15290)
                     /gene="pstB"
                     /locus_tag="VVE42_000597"
     CDS             complement(14538..15290)
                     /gene="pstB"
                     /locus_tag="VVE42_000597"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU3072844.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWDEVKDNLKKSAMSLSGGQQQRLCIARA
                     LAVEPDVLLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            complement(15292..16107)
                     /gene="pstA"
                     /locus_tag="VVE42_000598"
     CDS             complement(15292..16107)
                     /gene="pstA"
                     /locus_tag="VVE42_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEU3072845.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDSMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            complement(16100..16963)
                     /gene="pstC"
                     /locus_tag="VVE42_000599"
     CDS             complement(16100..16963)
                     /gene="pstC"
                     /locus_tag="VVE42_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEU3072846.1"
                     /translation="MTKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFIANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRTFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            complement(17081..17956)
                     /locus_tag="VVE42_000600"
     CDS             complement(17081..17956)
                     /locus_tag="VVE42_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HEU3072847.1"
                     /translation="MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            complement(18123..19454)
                     /locus_tag="VVE42_000601"
     CDS             complement(18123..19454)
                     /locus_tag="VVE42_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEU3072848.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGLGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSGLKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            complement(19455..20162)
                     /locus_tag="VVE42_000602"
     CDS             complement(19455..20162)
                     /locus_tag="VVE42_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU3072849.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGMEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGEQEGDDSDNIADDSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            complement(20246..20731)
                     /locus_tag="VVE42_000603"
     CDS             complement(20246..20731)
                     /locus_tag="VVE42_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU3072850.1"
                     /translation="MAPTDKEDLIRTLELAPHVEGGYFRQTKKASDTINLAGKGRALY
                     TSIYFLLEETNPSHFHRLTADEIWYFHAGSPLTVHMITADGHYEAVTLGLDISKGQQL
                     HYCVPKGTIWGSTVDKDDALVSCLVAPGFEFEDFELFERVDLLATYPEHKEMIERLTR
                     Y"
     gene            20717..21275
                     /locus_tag="VVE42_000604"
                     /pseudo
     CDS             20717..21275
                     /locus_tag="VVE42_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(21590..23281)
                     /locus_tag="VVE42_000605"
     CDS             complement(21590..23281)
                     /locus_tag="VVE42_000605"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEU3072851.1"
                     /translation="MNTKELIASELSSIIDSLDQEAILKLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIAAELAEKMNSQAFEKVVATGPYVNFFLDKSAISAQVLQAVTTEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGDEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NGDEEALALWQWFRDESLVEFNRLYNELKVEFDSYNGEAFYNDKMDAVVDILSEKGLL
                     LESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTVAEAVSR
                     AKVQIEAKNPELENKDQVAHAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPEIAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQSFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
BASE COUNT         6743 a         4509 c         5052 g         7048 t
ORIGIN      
        1 gaaataagta tgttaaaaga agtattaacc gttgcaaaag ttgcgaaaaa atcttcatta
       61 tttttgggtg gtgtcgcatt tggtaccctt ggtttgaaaa tcttagcaag taaggaagct
      121 aaaaaaggtt attctaaagc tttggctaag gcttacaagt tgaaagacga gctagatgca
      181 tctgtttctg ttgtgaagca acatggagac gatgtcttgc aagatgccaa atatttgtac
      241 gagcaagaga aaaaagaaga gcaattagat agccttatag gtgaataata tgtcttttaa
      301 agtgctacat agaggatacc aacatatccg actatcatct tctttttcac ttaccttgga
      361 tattcaagac tatcttcgtt ccttggcgag agatgaaaag gggattgagt ctatccagtt
      421 ttacatggat caacagcact ttactctacg cataaaagaa ggcttttctg tattagataa
      481 tgcagaagcc tttttaaaaa gaattgataa agggaaagtt tctgagttga tgactcttcc
      541 cattcgtaga gaagagagtg cttattctat tgtttcaggt gcagcggtta agcgtgtact
      601 ttttcgtagt tttgtgccgt atcctattcg ctatatatgg acttgttatc aggctttggg
      661 ttatattaga gaagcctatc aaacactagc gcgtaaggaa ctaacgatgg aagtcttgga
      721 ctgttcggct attttattgt ccttgtttat gaaccaatcc aagacagcta gcaatatcat
      781 gtttatgctt gatttgggga atcatttaga tcagtggtcc ttgaaaaaaa ctgcaacaga
      841 tttagaacag agtcttcttg caaaagagag cgatgtattc ctagtacagg gcgatacggt
      901 tgttagtatc aagagttccg atgttcaaat aggagatgtc ttgatcttat ctcaaggaaa
      961 tgaaattctg tttgatgggc aagtagtttc aggtttaggt atggtcaacg aaagttcctt
     1021 gacaggagag agttttccag ttgaaaaaag agagtctgat ttggtttgtg caaatacagt
     1081 attagaaact ggagagttac gcattcgtgt aacagataat cagatgaaca gccgtatttt
     1141 acagctgatt gagttgatga agaaatctga agaaaacaag aaaacgaaac aacgctattt
     1201 catcaagatg gcggataaag tcgtcaaata taatttcttg ggggctgggc tgacttacct
     1261 attgacaggt tctttttcta aggctatttc tttcctattg gtcgatttct cctgcgcttt
     1321 gaaaatctct actcctgtag cttatttgac agttatcaag gaagggttga accgtgaaat
     1381 ggtgattaag gatggagatg ttctggagaa atatctggaa gttgatactt tcttgtttga
     1441 taagacagga acaatcacaa ctagttatcc tatagttgaa aaggtgttac cttttggaga
     1501 ctatagtgag gaagatattc tcagaatcag tgcctgtctt gaggaacaca tttatcatcc
     1561 tattgctaat gccatcgtca agcaagctga gatagaggga attgaacatg aggaaatgca
     1621 tgggaaactc caatatatcg caagcaaggg gatcaaatct catatagatg gccaaccagt
     1681 tcttattggg aattatgtct tgatgcagga tgagcagatt catatcagtt cagaacaaaa
     1741 tgctttaatt gaagagtaca agagtcacta caatctctta ttcttggctt atcaaaatga
     1801 attgattgga atgttctgca ttcatactcc tttgagaaaa gaagcaaaaa cagccttgga
     1861 taaacttaag gcacagggga aaaaattgat tctggcaaca ggggacacct tggttaggac
     1921 aaaggaatta gtcaaagatt tgccctttga tcaggtctat acagacttga aacctgatgg
     1981 gaaatttgag ttagtagaga aactgcagaa agcaggtcac actattttga tggttggaga
     2041 tggattgaat gactcagcgg ctttaactct atcagatatc ggtgtggtga tgaatgagag
     2101 tgcagatatt tctaagcaga tgagtgatat cttattgtta gataatcgtt tggatttctt
     2161 ccaagagttg gattcgttat catcatcttt gcaaacactc atcaagaaaa atattcaaga
     2221 taccgttgtc gtaaatagta gtttgattgg cttcggctta tttaattggc tcaatccttc
     2281 aaatctctct atcttacata atctaacaac cttgcgcatt gtcctgcgta gcctgtctat
     2341 taaaaataga tagatgagag ttattaacag caaaaaggag ttacctttct tgaggctaac
     2401 tcctttgttt atagataaat gttgaatagt ttagtaagtc aatacaaagt attttttctt
     2461 cccacgacgg ataacagtca gttcattttc taacttatca gcgtcactca agacatagtc
     2521 aaggtcttgg atgcggtcgc cgtttacgta gatggctccg ttttggacgt cttcacgggc
     2581 ttggcgtttt gagttaacta taccagatga gacgagcagt tccacgatat tgttgtgttc
     2641 gtctgcctgt acttggtagt ttggcacacc acgaagtcct tgtttgagct ctttgacaga
     2701 aaggtttttg atgtttcctg caaagagttg ctcagtgatg ttaagtgctt ctttgtaggc
     2761 ttcttctccg tgaacaagtg taacaacttc acgagccaag actttttgag ccaagcgttc
     2821 gtgtggtgct gcttcaaatt gtttgcgaat atcttcaatc tcatcaagtg acaagaaagt
     2881 aaagattttc aagaagcgaa cagcgtcagc gtccatcacg ttcatccaga attggtacat
     2941 ttcgtatgga gaagtctttt cgggattgag ccagacggca tttccttctg atttaccaaa
     3001 tttcttacca gttgcatctg tgattagtgg aacagtgata acgtgaccag tcttgtccgc
     3061 cttacgacga agcaattcgg taccagctgt catatttccc cactggtcag aaccaccgat
     3121 ttgaagagtg acattatggt cttggttaag gacgaagaag tcataccctt gcatgatttg
     3181 gtaagcgaac tcagtgtaag aaattcctgt ttcgatccgt tttttaacag attccttact
     3241 catcatgtag ttgaccgtga agtattttcc aatatcacgg aggaagtcaa tgaagctgat
     3301 gctgccaaac cagtcgtagt tgttgaccat gacagccttg ttttcgccat tttcaaagtc
     3361 aagaaaacga gaaagttgtc cttggataga cttgacccag ccatctactg tgtcttttgt
     3421 ttggagacta cgttcagcat ctttgaagga cggatctccg atgagacctg tagcaccgcc
     3481 aacgagcgca taaggtttgt gacctgctag ttgcaagcga cgacttgtca agattgcgac
     3541 aaggtggcct aggtgaaggc tgtcagcagt tggatcgtag ccagtatagt aagaaacttg
     3601 accttcttct agggctttac gcaaagcttc ttcatcagtc gtttgaaata tcaaaccacg
     3661 ctcttttagc tcatcaaaaa tgtgcatgtg tcttttctcc tttttaaaat tattgtttct
     3721 acctattgta tcacaaactt gggcaatagc ctagcagaat agggaagaaa gtggctattt
     3781 tattgaaaat ttcccttttg tggtataata gatagagtga ggaaatccat gcaaaatcaa
     3841 ttaaatgaat taaaacgaaa aatgctggaa tttttccagc aaaaacaaaa aaataaaaaa
     3901 tcagctagac ctggcaagaa aggttcaagt accaaaaaat ctaaaacctt agataagtca
     3961 gccattttcc cagctatttt actgagtata aaagccttat ttaacttact ctttgtactc
     4021 ggttttctag gaggaatgtt gggagctggg attgctttgg ggtacggagt ggccttattt
     4081 gacaaggttc gggtgcctca gacagaagaa ttggtgaatc aggtcaagga catctcttct
     4141 atttcagaga ttacctattc ggacgggacg gtgattgctt ccatagagag tgatttgttg
     4201 cgcacttcta tctcatctga gcaaatttcg gaaaatctga agaaggctat cattgcgaca
     4261 gaagatgaac actttaaaga acataagggt gtagtaccca aggcggtgat tcgtgcgacc
     4321 ttggggaaat ttgtaggttt gggttcctct agtgggggtt caaccttgac ccagcaacta
     4381 attaaacagc aggtggttgg ggatgcgccg accttggctc gtaaggcggc agagattgtg
     4441 gatgctcttg ctttggaacg cgccatgaat aaagatgaga ttttaacgac ctatctcaat
     4501 gtggctccct ttggccgaaa taataaggga cagaatattg caggggctcg gcaagcagct
     4561 gagggaattt tcggtgtaga tgccagtcag ttgactgttc ctcaagcagc atttttagca
     4621 ggacttccac agagtcccat tacttactct ccttatgaaa atactgggga gttgaagagt
     4681 gatgaagacc tagaaattgg cttaagacgg gctaaggcag ttctttacag tatgtatcgt
     4741 acaggtgcat taagcaaaga cgagtattct cagtacaagg attatgacct taaacaggac
     4801 tttttaccat cgggcacggt tacaggaatt tcacgagact atttatactt tacaactttg
     4861 gcagaagctc aagaacgtat gtatgactat ctagctcaga gagacaatgt ctccgctaag
     4921 gagttgaaaa atgaggcaac tcagaagttt tatcgagatt tggcagccaa ggaaattgaa
     4981 aatggtggtt ataagattac tactaccata gatcagaaaa ttcattctgc catgcaaagt
     5041 gcggttgctg attatggcta tcttttagac gatggaacag gtcgtgtaga agtagggaat
     5101 gtcttgatgg acaaccaaac aggtgctatt ctaggctttg taggtggtcg taattatcaa
     5161 gaaaatcaaa ataatcatgc ctttgatacc aaacgttcgc cagcttctac taccaagccc
     5221 ttgctggcct acggtattgc tattgaccag ggcttgatgg gaagtgaaac gattctatct
     5281 aattatccaa caaactttgc taatggcaat ccgattatgt atgctaatag caagggaaca
     5341 ggaatgatga ccttgggaga agctctgaac tattcatgga atatccctgc ttactggacc
     5401 tatcgtatgc tccgtgaaaa gggtgttgat gtcaagggtt atatggaaaa gatgggttac
     5461 gagattcctg agtacggtat tgagagcttg ccaatgggtg gtggtattga agtcacagtt
     5521 gcccagcata ccaatggcta tcagacctta gctaataatg gagtttatca tcagaagcat
     5581 gtgatttcaa agattgaagc agcagatggt agagtggtgt atgagtatca ggataaaccg
     5641 attcaagtct attcaaaagc tactgcgacg attatgcagg gattgctacg agaagttcta
     5701 tcctctcgtg tgacaacaac cttcaagtct aacctgactt ctttaaatcc tactctggct
     5761 aatgcagatt ggattgggaa gactggtaca accaaccaag acgaaaatat gtggctcatg
     5821 ctttcgacac ctagattaac cctaggtggc tggattgggc atgatgataa tcattcattg
     5881 tcacgtagag caggttattc taataactct aattacatgg ctcatctggt aaatgcgatt
     5941 cagcaagctt ccccaagcat ttgggggaac gagcgctttg ctttagatcc tagtgtagtg
     6001 aaatcggaag tcttgaaatc aacaggtcaa aaaccaggga aggtttctgt tgaaggaaaa
     6061 gaggtagagg tcacaggttc gactgttacc agctattggg ctaataaggc aggagcgcca
     6121 gcaacaagtt atcgctttgc tattggtgga agtgatgcgg attatcagaa tgcttggtct
     6181 agtattgtgg ggagtctacc aactccatcc agctccagca gttcaagtag tagttctagc
     6241 gatagcagta actcaagtac tacacgacct tcttcaaggg cgagacgata attctctaaa
     6301 tgaagtggcc aatcaatgga ttgccacttt tttcctttcg tgttacaata agggtatgaa
     6361 tcagtatcag aaaaagattg ttaacggaaa aatttattcg ctcctatccg gcttaatatg
     6421 gggaatctgt ggaattttag gagagtactt ctttactcat tatcaggtgt cttcgggctg
     6481 gattacctct atgcgtttga cactggcagg gagtcttgta ctcatttggt ctgcaataca
     6541 attaaaatcg caagtgctag atatttggcg agacaagaaa aattacctgc cctttttagc
     6601 ctatgctatt ttggggattt tttcagttca gtattttttc tatctctgtg tagaatactc
     6661 aaatgctacg acagcaacta ttttacagtt tattagccct gtctttatcc tcttttataa
     6721 tcgcttggtt tatcaaaaac gagcgtcaaa aagcgctgtt ttctatgttt tggttgccat
     6781 gctgggtgtt tgcttgatgg cgacaaaggg agatctctct cagttatcca tgacgccgct
     6841 agctcttata acaggtttgc tgagtgccat gggtgttatg tttaatgtta tcttgcccca
     6901 accttttgct aagcgttatg gttttgttcc tacggttggg tgggggatga ttttggcagg
     6961 tttgtttagc aatgtcctct cgccggttta tcagctttcc tttactcttg atatttggag
     7021 tatcttgatt tgcctcatta tcgctttctt tggaacggct tttgcttttt tcatttccat
     7081 gaaggctgtg tccttggttt ctcctttggt ggtttccgtt atcagtgcca gtgaacctct
     7141 ctcttctgct ctcttgagtg ttttgttctt aggattagta gtggattggt ccttccttct
     7201 agctatagcc ttgattattt tacccatgat atttttgtct atagaagaag cgaaagaaag
     7261 tagataaaaa gtcttttctt aattctaaaa gtgtgctata ctagaatagt caataaaaca
     7321 cggggagata gttgtgaaga gtgtttttag gttgtatcgg taggggcttg cttttaccga
     7381 cagcacgttt tatctcacat ccctaaaaaa tcatacctaa aaacagacaa aaaggtttca
     7441 aatttgaggc cttttttggt agaataggta tcattataac gaaccaggag gcacctatga
     7501 ctgctacaaa aatgaacgct caagaaatta tccaatttat cgccaatgct gaaaagaaaa
     7561 ccagtgtcaa agtaaccttt gaggggcaac tcgcaactgc tgtgcctagc tctgttgtca
     7621 aactagggaa tgtcctattc ggggattgga aggacgtggc tccgcttctt gaaggtttag
     7681 tagaaaatca agattatgtt gtcgagcaag atgctcgtaa ttctgcagtt cctttgctag
     7741 ataagcgtgc tatcaacgct cgtatcgagc caggtgctat tatccgtgac caggtggaaa
     7801 ttggtgacaa tgttgttatc atgatgggag ctgttatcaa tatcggtgct gaaatcggtg
     7861 ctggaaccat gattgacatg ggtgccatcc ttggtggccg tgccatcgtt ggaaaaaata
     7921 gccacgttgg tgcaggtgca gttttggcag gtgtgattga gccagctagt gctgaaccag
     7981 tccgtgtcgg agacaatgtt ctcatcgggg ctaatgcagt ggttatcgaa ggagtccaaa
     8041 tcggtagtgg ttcagttgtc gcagcaggag ctattgttac ccaagatgtc ccagaaaacg
     8101 tggtagtagc aggtgttcca gctcgtatta tcaaagaaat tgatgcccaa actcaacaaa
     8161 aaacagcgct agaggatgcg cttcgtacct tgtaattgta aaagtaaaaa agaggcggaa
     8221 ccctttttcc agcctctttc tgctatatcg gaggatagat agatgttaga tttgattcag
     8281 actagacgag atttacacca gattccagag attggcttgg aggagttcaa gactcaggct
     8341 tatttgctgg atgtgattga gaaattgact acgggcaagg attttgttca aattcgtact
     8401 tggcggacag ggattttggt ctacctgcag ggaagtcagc cggagcgaac cattggttgg
     8461 cggacagata ttgatggcct gcctatcgtc gaacaaacag gtctaccttt tgcttctcaa
     8521 caccaaggtc gcatgcatgc ctgtggccat gattttcata tgaccattgc cttaggctgt
     8581 cttgagcgcg cccttgagga gcaacctaaa aataatctgc tctttctatt tcagcctgct
     8641 gaagaaaatg aagctggtgg tatgctcatg tatgaggctg gtgcttttgg agattggttg
     8701 ccagatcaat tttatggcct ccatgtccgt ccagatttga aagtcggcca gattgcgact
     8761 aatactcata cactctttgc aggaacttgc gaggtgaaga tccgtttcaa aggtaaaggt
     8821 ggccacgcgg cttttcctca tgaagccaat gacgccttgg tggcggctag ttactttgtg
     8881 acccaagtgc agtcagttgt tagccgcaat gtcaacccaa tcgagggagc agtggtgacc
     8941 ttcggcgttt ttcaagctgg aaccaccaac aatgtcatca cagacacagc ttttttacat
     9001 ggaactattc gtgccttgac acaggacatg agtctcttgg tgcaaaagag ggtaaaaaca
     9061 gttgcagaag gggttgcagc tgcctttgat atggaagtcg aagtggaact caaacaaggt
     9121 ggatacctac ctgttgagaa caatccagcc ttggcgcgtg aactgatgga cttctttgac
     9181 gaaaaagacg gaatcgagtt gattgatatc gaacctgcta tgacaggcga ggactttggt
     9241 tatctccttt caaaggttga tggcgtgatg ttttggctag gtatcgatag tccctacgct
     9301 cttcatcacc ctcaaatgag tcctaaggaa gaagtcttag ctattggggt ggctgcggtg
     9361 tctagtttcc tgaaaaagaa ggcagcagaa tagagggctt gtctatgaaa tcggaattac
     9421 gcaagcaagt cttgcaagga atgaaggcta tatctcaaga acaaaaacag tttatagatc
     9481 aaactttaac cgagcggctt ttacaccatc ccttctatca agaagccaag gtcatcgcaa
     9541 cctatctctc ttttcctcat gagtttcaaa cgcaggaact gattgagcag gcgctgaagg
     9601 acggcaagaa ggttttaata cccaaaactt atcccaaggg gcgcatggac tttgtggtct
     9661 atgatccgca acagttggta aaaacttcct ttggattact ggagccacag ggagatttgg
     9721 aagtggtgga tgcatctcag attgatttga ttcatgttcc tggcctggct tttacgacaa
     9781 aaggatatcg tataggatac ggtggaggct attatgaccg ctatctggaa catttttctg
     9841 gtcatacttt gagtacggtt catccttgtc aaattcagga ctttatacct gaaaaccatg
     9901 atattcctgt tcaggaggta ttaattgatg aaggaaatct ttgatagacg ttaccctgtg
     9961 acgagtttct tcctcttagt gacggccttg gtatttttac taatgttggt cactgcaggc
    10021 ggaaactttg acagggcaga tacattattt cgatttggag ccatgtatgg gccagctatt
    10081 cgcctctttc ccgagcaggt ttggcgtctc ttgtctgcca tttttgttca tattgggtgg
    10141 gaacatttca ttgttaatat gctttcactt tattatcttg gaaggcaggt agaggagatt
    10201 ttcggttcta agcagttttt ctttctctat cttttatcag gaatgatggg caatctcttt
    10261 gtttttgtat ttagtcctaa atccttagca gcaggcgcct ctacctctct ttatgggcta
    10321 tttgccgcga ttatcattct tcgctatgca actcgcaatc cttatatcca acagctaggg
    10381 caatcctatc tgacactttt tgtggttaac attattggaa gtgttctgat tccaggaatc
    10441 agcctagcag gccatatcgg tggtgcagtt ggtggcgcat ttctagcagt tatctttcca
    10501 gttagaggag aaaaacggat gtataatacc agccagagat taggagcggt agtcttgttc
    10561 gtaggactcg ccattttgct tttctacaag ggaatgggaa tgtgacgaag ttgaaagaaa
    10621 atttgtgtaa tgaaaaaaga taaggcattt tttgaacctt atctttttat tttattttat
    10681 tccttctcag tcaattcttt tccaagttgg atgaggtaat tcttcaaatc atctttgact
    10741 tgtgggtgtt tgagggcgta gtcgatggat gttttcatga agccaaactt gtctccgaca
    10801 tcgtaacgag ccccattgaa ctcacgagca aatacacgtt gtgttttatt gagggtgtcg
    10861 attgcatctg tcagctgaat ttcatttcct gcacctggag cttggttttc gaggatttgg
    10921 aaaatttcag gcgtgaggag gtagcgtccg ataatagcaa ggtcgctagg agcgtcctct
    10981 ggagctggtt tttcaacaaa ggtttcaaca ctgtaaagac catcttttcc ttcgccttgc
    11041 ggagcaataa ccccgtaagc agatacttcg tcatgaggga ctggcatgac agcgatagta
    11101 gacgcgtggg tacgctcgta gtcatccatg agttgtttgg taagtggaac agccttttcg
    11161 tctgtgatat ccatcaagtc atcaccaagc ataacgacaa aaggttcatt tccgacgaaa
    11221 gccttggctt gcaaaacagc atctccgaga ccgcgtggat gagtttggcg gataaaatgc
    11281 agacgcatgt cagttgtttt atcaactagc ttcaaaagat ctgttttccc tttttctttg
    11341 aggttatatt ccaattcgaa gtttgaatca aagtggtcct caatagaacg ttttgattta
    11401 ccagtgacaa ctagaatatc ttcaatacct gatttgagag cctcttccac gataaactgg
    11461 atagttggtt tgtctacgat tggcaacatt tctttggcaa gggccttggt tgctggtaaa
    11521 aatcgagttc ctagtccagc agcagggatg actgcctttc taacttttga tgtcataaaa
    11581 atcctttcta tagagggtta agaccactca ttttctgctt taaattcatt gttcatgatg
    11641 tcataaatgg catctttgat attggttccg tgataaataa cttggtaaat agcctgtgta
    11701 atgggcatat agactccaag ttcttgggct agttcatagg ctgctcgagt cgttgaaatt
    11761 ccttcgatta ccatgcccat attagcttct atatcagcta gggattctcc tcgtccgaga
    11821 gcatctccag ctctccagtt acgagagtgg atggaagttc ccgttacgat caaatctccc
    11881 acaccagata agccgctata ggtcaatgga ctggccccga gtgctacccc taggcgggtg
    11941 atttctgcta aacctcgagc gatgatggct gccttagcat tatcaccaaa tccaagacca
    12001 tgtaaagctc cagcaccgac agcaataata tttttaagag caccagcagt ttcaaccccg
    12061 ataacatccg tattggtata aagtcggaag tagtgattac taaatagctt ctgaacgtat
    12121 tgagctgttt gtaaatcttt agaagcagca gttattaaag ttaggtcacg cacaatggtc
    12181 tcttctgcat gactaggccc tgaaacaacg acgatatcac tacggagatg ttcaggaatt
    12241 tcttcttcaa gaatggttga taatcgttta tggctatcag gttctaatcc ctttgatgcg
    12301 tgcatgatga taaccttatg gtccaaggtt tgtgcaactt gctgggcaac aagtcgtgtc
    12361 acttttgttg ggacaacaaa caaaatcgca tccacatctt tcaatgtttc tgctaagtcg
    12421 gtgtaggcaa tgatattttc gtctagaacg acatctttaa agtagtgctt attagtatgg
    12481 tgtgtattaa tttcattgat ttgctcggga agatttcccc aaatacgtac ctcgtgtcca
    12541 ttgtcattta agacttgtga aagggcggtt ccccaagaac caggccccaa gacggcgacg
    12601 gtttgttttt ccatgatttc ctccttgata gagttctttc tcttattcta tcatattcta
    12661 ggggattttg cacttgcatt actggggtat ggtggaaaaa tacacaaaaa ccgagatgaa
    12721 gggataaact cttagtcttt agttaaagtg agaagaaatc tgatagatag tttgattcag
    12781 tatatgctat aaatagaggt aaacaactca ggaggttctt gtaggttgcg agagtttggc
    12841 gaaaaaatta aaagattacg tttggctaaa aaaatcagtc gttcagaatt ttgtggtgat
    12901 gagtctgaat taagtatccg tcaattaatt agaattgaaa atggagaatc cagaccaaca
    12961 ctaacaaagt taaaatatat cgctgaacgt ttgggggttg aagattacaa gttgatgcca
    13021 agttatatag agttggataa ggaataccta gaattgaagt atttcttgat gaggactcct
    13081 acatacgaag atgaaactat cgcccaaaag aaagagagtg ttttgataag atttttgaag
    13141 agtattatga taggctacct gaggaagaaa gatttatcat cccaaattat tcatatctgg
    13201 cactaacgaa ctacacagtt caaaaattac cagaaaagct agttgaaata ctgtccttct
    13261 ggtgattttg cttaatttct gatgttcgag acataattga ggagatttgt cttgattttt
    13321 tcctaaaaaa gggcagactc ctcccttggt ttgatgctcg atttttaaaa taaggtgact
    13381 tattctgtta ggcatgtttt cccctgccct ctagctctat atggaacagg gagtatcaga
    13441 tttatctggc agattgtatc aaataaggct tgaaactgtt ttgaataggc gtatagactt
    13501 gagaatttga gaatatgtcg tctagcagta gagacatatt ttccggcaat atgaaggaag
    13561 agacgtcgaa aacgcttgat agtcaaggcc tttacttcat caccagctag ctgctttaaa
    13621 aagaggtaga gattataagc cagacagccc atcatcatac gtacttcatt cttaatcatg
    13681 gtcgaactat ctgtcttatc cccaaagaat cctgctttcc tttctttgat aaagttttca
    13741 gcattccctc gttcacgata gaattgaaac agctcctcac tatctacact gcttaaattg
    13801 gtcaaatgaa cttttttaca accataaaaa agagcactta atcgtactct tctcttttaa
    13861 ggattagttg aattaattca aatccactag ttctcctgtt tctaggtaga ctaggcgttc
    13921 acaaatgtta gcaatgtaat cagcgaagcg ttccagatgc cctatgatat aaaggtattg
    13981 agttccattg ggaattgagg tttcttggtc tttcataagt ccaatgattt cctttgataa
    14041 ggcataataa tattggtcaa tctgttcatc tttttgagca atactaatag cttttgaggc
    14101 ttggtgcaaa ggaaaggcaa ccaataaatc ggctagcatg ctgagggata atttacccat
    14161 ttggtgtaac tgttcttcgt caggggctag ttgattttct tttagttgca aaacagcttt
    14221 ggcaatgcct gccatatggt ctcccatacg ttcaaggtct gaacaagaag acatgatgct
    14281 aattacaaat cgaaggtcag acacttgtgg ctgctgcaag gccaacaaac gggcacaggt
    14341 caattcgata gcgctttgac cttggttgat agcatgatcc ttattgataa ttagctctgc
    14401 catctccttg tctttggagg ctaaggccag taaggctttt gaagctgttt caaggacaag
    14461 ttgccctagt cctaaaaagg attgttctaa ttcatgcaat tctaagtcaa attgatttct
    14521 cataggtttt tccttcctta tccgaaccgt cctgaaatat agtcttctgt gcgctgatct
    14581 tttggattgg taaacacgtc aacggtatct ccaaattcgc aaatttctcc tgttaagaaa
    14641 aaagcagttt tatctgaaat acgtgaagct tgttgcatgt tatgggtaac aatgataatc
    14701 gtataatcct tttttagttg ctgaatgagg tcttcaattt ttaaagtgga gatagggtct
    14761 aaggctgaag tcggctcatc cattaataga acatcaggtt ctactgctaa agctcgcgca
    14821 atgcaaaggc gttgctgctg accgcctgat aaggacatgg cactcttttt aagattatct
    14881 ttgacttcgt cccaaatggc tgcccctttt aaagattttt ccactaaggc atctaattgt
    14941 tttttgtctc gaataccatg tgtccttggg ccataagcca cgttatcata gatagacatg
    15001 gcaaagggat taggctgttg aaaaaccatc cctacacgct tgcgtagctg attaaggttg
    15061 aatttgctac tataaatatc ttgctcatct aagaggactt ggccttcaat atggcaagaa
    15121 ggaaccaaat cgttcatccg attaagggtt tttagaaaag ttgatttgcc acaaccagat
    15181 gggcctatca aggcagtaat ctgtctttct ggtaattgaa tcgaaatatt ttttaaggct
    15241 tgaaaatccc cgtaaaataa gtctaggtgt ctgactgaaa atgttcccat actaggaagc
    15301 tcctttcaca agtttccgag ataataagct tgatagagta tttatcatta aaacagtaat
    15361 aattaaaatc acgccggtag cataggcttc attgacatgt agcccctcac ttgacagcat
    15421 atacatatgt agggctagag aacggcctga agacatgaga ctacttggcg tattggtaga
    15481 ggtacctaat gtatacatga gggcagctgt ttcaccaacg atacggccaa tagctagtat
    15541 cactccagct aaaatacctg gcatggcaac tggtagaaca attctaaaaa cagtccgtaa
    15601 cttacctgcc ccaagtccat aacttgcttg acgcatgcta tcactaacag ataaaagggc
    15661 ttcttctgtt gagcgaataa tgactggcaa caccatgatg actgaggtta agattcctga
    15721 taacagagag tattgaaaac ctaagaagac tacaaagaag agcatgccaa acagaccaaa
    15781 aacaatggaa ggaatcccag acaaggtatc tgaggccaat cgcatgattt taacacaaag
    15841 ggaatctttt tttgtatatt ccacaagata aaaaccagca aaaatcccta tgggcaaggc
    15901 taaaagaaga gcaccaaaga ccagaataac ggtggaaata atcgctggca taagggaaat
    15961 gttctcagaa gtataagtcc aagaaaagag ggatagactt agatgaggta agcctttgat
    16021 gaggataaaa ccaatgatta aaaagagaga gccaaaggtt aaagctgaaa aacaataaac
    16081 gagaagtttt agcaggtatt tactcataag atgattttcc tttcaagtag gcaaagtagg
    16141 cattaatcaa gagaataagg aaaaagagaa ctgctgaggt tgcaataagg gcttccctat
    16201 gctgacctga tgcgtaagcc atttccagaa caatattggt tgttaaggtt ctggttcctg
    16261 aaaagagtcc acttggaata atcggctggt tgcctgccac caaaataact gccatggttt
    16321 cacctactgc gcgaccgatt cctaaaataa ctgctgataa aataccagat ctcgcagctg
    16381 gcaagatgac actaaaaata ctccgttcat gactagctcc tagagccaag ctaccagaat
    16441 aatacgtttt gggaactgtt cggatagcag attctgacaa actgataatg gttggcaaaa
    16501 tcattattcc taatagtaac gaagcggtta ggacactcat gccatttcct aaaaaggttc
    16561 taatccaagg caccaataat tgtaggccga aaaaaccata aacaatagat ggaatggccg
    16621 ccatcaagtt gatagctgat tttaagaagc catagacggg ctttggacaa taataaacca
    16681 taaacaccga tgtcaagatg cctgttggca ccccaatcac aatcgctcct aaggtaatta
    16741 ataaggaacc aacgatcatt ggtaaaatac catagcttgc cggaatgttc gttggcgacc
    16801 aatcactgcc taataaaaaa cgggcaaagc cgtagttagc tatgaaaggt aagccattac
    16861 taaaaataaa gaaacagatt agcaaaatag ctacaacagc tactgttgca ctcatgaaaa
    16921 aaattgccct aaaaactgct tctttgaagg cttgttttgt cacatcttgt cctttctagt
    16981 gaagaaagta agggagatac gacacctccc tacttgcctt ctttatctta ttgtacgatg
    17041 aaacgtctgc atctctttag agatttatgg agcaaacatt ttatttaatc ttgtcccagg
    17101 tggttaattt gccactaaaa acgtctgcaa gttcagccat actgacttgg cttgccttat
    17161 tgtcattatt gaccacaaca gcaataccgt ctaaagcaat agcatcatgg gtgagactct
    17221 taccttcttc aggagttaat tccctagaaa ccataccaat atcagcggtt ttctccttaa
    17281 cagcggtaat acctgctgaa gacccattag aggtaatatc aatcgtaact tctggatttt
    17341 cttttttata agcttctgct aatttttcca ttaaagaaga tactgaagtg gaacctacaa
    17401 cagacaactt gcctgataag tgttggcttg tatattctgt ggtttcagtt ttagcttcaa
    17461 taaatttatt atctgtgacc acttgttgac cttgtttgga gtggataaag ctgataaaat
    17521 cttgacctag cttggaaaga ttagaagacc aaacaatgtt gaagggacgt tgaagagggt
    17581 attcaccatc taaaactgtg tctcgactag ccttgacacc atcaatctct aaagccttga
    17641 cagatttcgt taaagatccc aaggagatgt agccgatagc attagcattc ccttgaactg
    17701 ctgagagaac accttctgta ctattttgaa tcacagctgt tttggcagtg ttgtcaattt
    17761 ttttatcacc gtcttttttg agaatccctg tgatttctgt gaaggcaccc cgtgttccag
    17821 agccattttc tcgtgaaatc acctcaatcg ttcctgaagc tgactgtttg gaagcagctg
    17881 actgattgcc acaggcaaca agcccaaatc ctgataagcc aatggctgca agagtaagca
    17941 tttttttgaa tttcataata atcaccttta tctctatgta tttttcttgt gtaggcttac
    18001 tacatttata gtctaacaag tctttgtaaa ggtttatccc tgattcatgt aaagattgtg
    18061 taaagaatca aaaaaagcca cttttgaaaa atggctgctc ctaaaaatag ctttaaaaat
    18121 tattagtcct gtgcgaaaga ttggttagga agaaaaatcg tgaagcaact gcctctgcca
    18181 agctgactcg tcaccgtgac ttggccacct aataattgac tgagttcttt gacaatggca
    18241 aggccaagac cagtgccacc agtttgtctg cttcgacctt tattaactcg gtaaaaacgt
    18301 tcaaaaatac gatcctgctc taattgacta ataccaatcc ctgtatctga tacagaaatc
    18361 ttaatgcctt cgttcacctt ttgggtcttg acctcaattt ttcccccttg ttcagtgtaa
    18421 cggatggcat tggataaaag attgagtaag atttgggaaa gtaattgact atctgatacg
    18481 agggtgacat catctggcac ctgcaccttt agctgtaaat ccttcttctt gagctgaggt
    18541 tgcaagcttt gagtcaaatc ctgtacaaat tcagccaaag aaagggtcgt ccattgtata
    18601 ggcatttgtt gagccttaga taaggtaaga agatgctcaa caatatgctc aagacgcaaa
    18661 ctttctttgt aaataatgtc tagaaagtca tccttgagcg cttcttcttc agctgacatc
    18721 cccttaatgg tttcagcaaa gcccttaatt gaagtaactg gtgtcctcaa ttcatgggag
    18781 gcatttgaga caaaggctaa atttaacttt tcataagttc taatcgttgt taaatcatat
    18841 agcaagacga gcacagcttc cacagattgg gtggggctaa aaacgggaac tgctgtcact
    18901 tctaaaatca agtcaccctc atgaaaccca cttacttctt gttttaacct tgttttttga
    18961 tcaaaggctt ggtgaactaa atttcgaata tccatccgtt tgaggtcatc aagtgaactt
    19021 atgtcgccgt ccacatcggg aaaataatga ggcagagagc gactggataa taacatctga
    19081 ccttgagcgg aaactaaaaa cgtccccatg gttaggtgcg acagaagaac ctccattgtt
    19141 tcggctagat ccttgtattg ctgatcctgt tgggagactt tggtttttag gccagacaca
    19201 tactgagcca aagactttaa gtcttcttgc cctttttcta aaaagtattc actactggtc
    19261 aagagaggtt ggtgcaaggt ctcaaaagca acttcccatt tccaaaggca aaagagccag
    19321 tagccaccta gtcccaaaga aagggctaga agaaagagac cgatgccttt actgatccaa
    19381 gttaatgcca tccctgcaat cagaatgagg ctaacactta gattgactag ccaaaattga
    19441 aggtagcgtt tcatctataa ctccttgaac ttataaccat aaccccgaat ggttcgaata
    19501 aattgagggg ctttaggatt gtcttcaatt ttttccctca acttaccaat atgaacgtcc
    19561 accaaacgtg tttcctgccc aaagtcatac ccccagatac gttccaaaag acgctctcta
    19621 gtcagtgtca tgttgggatg tttcataaga tagagcaaga gttcaaattc ttttggggtc
    19681 aaactcagta acttattcgc cttgtagact tcatgacgct cagggtatac tttcaaggtc
    19741 ccaaatagcc aagaatcgtc agcgatatta tctgaatcat ctccttcttg ttctccttta
    19801 gttcgcctga ggacagcctt gacacgcgcc agcaattctc tagggctaaa aggcttggtc
    19861 aggtagtcat cagcccctaa ttccaaggcc aaaaccttat caaattcatc acttttcgca
    19921 gaaaccatca taattggagt tttgacgcct ttggctctca gccgcttaca aacttccatg
    19981 ccatctaatt gtggtaacat gatatcaagc aagataaaat caaagggttc tgtttctgcc
    20041 aaagctaagg ccttccgtcc atttgttacc aattgagtag aaaagccttc cttacttaaa
    20101 tggtagtcaa gcaatttcag aatgtgttct tcatcatcca ctaataagac ttgttttgtc
    20161 atctattatc tcctattggt aacattataa cacaattatc agaaatccta acattgctaa
    20221 atcagattaa atttgcttat caagactagt atctggtcaa acgctcaatc atctccttgt
    20281 gctctggata ggtcgccagt agatctaccc tttcaaataa ttcaaaatcc tcaaattcaa
    20341 aaccaggagc aacaagacaa gaaaccagag catcatcctt atcaactgtt gatccccaaa
    20401 tagtgccctt aggaacacag tagtgaagtt gttgcccttt ggatatgtcc aggcctaaag
    20461 tgactgcttc gtagtgacca tctgctgtaa tcatgtgaac agtaagtggg gatcctgcat
    20521 gaaaatacca gatttcatct gctgtcaatc ggtgaaaatg tgaaggattc gtttcttcta
    20581 ataagaaata aatactggta taaagcgccc ttcccttacc agcaaggttt atagtgtctg
    20641 aagctttttt tgtttgtcta aaatagccac cttcaacatg gggagctaac tctagagttc
    20701 ttatcaagtc ttctttatcc gtcggagcca atgggttgaa gtaactcttg ttcaaaggtg
    20761 gtgttacgat ttcaagaact cctctcagct ctgaggacac gataatgatt gatgcgacgg
    20821 aagtacaaat caatcgccct aaaagaagaa ttagcgaatt attctggtaa aaaaaatgcc
    20881 acgctatgaa ggctcaagcg attgtcacaa gtcaagggag aattgtttct ttggatatcg
    20941 ctgtgaacta ttgtcatgat atgaagttgt tcaaaatgag tcgcagaaat atcggacaag
    21001 ctggtaaaat cttggctgac agtggttatc aagggctcat gaagatatat cctcaagcac
    21061 aaactccacg taaatccagc aaactcaagc cactaacagc tgaagataaa gcctataacc
    21121 atgcgctatc taaggagaga agcaaggttg agaacatctt tgccaaagta aaaacgttta
    21181 aaatgttttc aacaacctat cgaaatcatc gtaaacgctt cggattacga atgaatttga
    21241 ttgctggtat tatcaatcat gaactaggat tctagttttg caggaagtct attatttggt
    21301 taggtgaatt agtgaagcgt ttaggcaagt gtctctggtt acgacgtcat ggcctctaaa
    21361 tcgattatat ttaggggtca tgactagtga agcagttagc tagttcgcat ataagcggct
    21421 agcgtctaac aattaggaac tttagttcca ataactttaa gattatgacg ttttaggaca
    21481 taaatcgatc atatttatgt cctaaaacta gtgaagcgcc tagccaaagt ccgaatagga
    21541 tttggcgtta gttacttaga ttgctttgca atcaagtaac tttggcgatt tacatcttct
    21601 ctggcgcttc tactccaagc aagcgaaggg cttctttgag aacgactgcg gttgcgtagc
    21661 tgagggctag acggctgtcg cgttctgggc tttcatccaa gatacgtgta tgtgcatagt
    21721 atttgttaaa ggattgagcc aggctaattg caaatttagc aatgatagaa ggttcaaagt
    21781 tatctgccgc acggttgata atacgtggga agtcttgaat gagtttaatg atttcccagc
    21841 tttcagtatc attcaagcta tagttgccag ctatttctgg tttgaaatcg gctttgcgta
    21901 agatagattg gatacgagcg taggcatatt gaacgtaagg tccagtttca ccctcgaagg
    21961 ataccatagc ttctaggtcg aagtcgtatc catttgtacg gtcagttttg aggtcataga
    22021 atttaatggc tccaacccca acagcatgtg ctacttggtc tttgttttct agttcaggat
    22081 ttttagcctc gatttggacc ttggcacggc taacagcctc tgcaacagta ggctctagca
    22141 agatgacatt ccctttacga gtagagagct tcttcccttc ttttgtaacc agaccaaaag
    22201 gaacgtgggt aatgtcgtca ctccaatcgt agcccatctc ttgcaagaca gctttgagtt
    22261 gtttaaagtg ggcagattgc tcttgaccaa cgacgtagat agatttagca aattggtatt
    22321 catttttacg gtagagggct gcagccaagt cacgtgtgat atagagagtt gcaccatcag
    22381 acttcttgat gagggctgga tgttcaattc catatttctc aagattcaca acttgggcac
    22441 cttctgattc aagaagtagt cctttttcag aaagaatgtc tacaactgca tccatcttat
    22501 cattgtagaa ggcttctccg ttatagctgt caaattcaac cttcaattca ttgtaaaggc
    22561 ggttaaattc cactaaactt tcatcgcgga accattgcca aagagcgaga gcttcctcat
    22621 ctccattttc aagtttacgg aaccattcgc gcgcttcttc atccaagcta gggtcatttt
    22681 cagcttcagc gttgatgcgg acatagagtt taaggagttc atcgattgga tgagctttta
    22741 cagcttcttc gtcgccccat tttttgtagg caacaatcaa catcccaaat tgtttacccc
    22801 agtctcccaa atggttgacc ttgaccgttt gataaccgat tttttggaaa atatgtgaca
    22861 agctatctcc gataacagtt gaacgcaggt ggccaataga aaatggttta gcgatattcg
    22921 gactagacat gtcgataaca acattttctt gtttgccaat attttggtca gcatagtgtt
    22981 ctttttcagt ggtaacagct tgcaatactt gagcagaaat ggcagattta tcaaggaaaa
    23041 agttaacgta aggtcctgtt gcgacaactt tttcaaaggc ttggctgttc attttttcag
    23101 ccagttcagc cgcaatcatt tgtggtgctt tacgttcgac ttttgcaaga gaaaaagcag
    23161 ggaaagcaat gtctcccatt tctgagtttt taggggtttc cagtaacttt aaaatagcct
    23221 cttggtccag gctatcaatg atgctagata attcgctagc aatcaattct tttgtattca
    23281 ttaagagctc ctttttggac ttttctacta ttttatcaca attttaaaga aagaagaaaa
    23341 aatttttgaa at
//
LOCUS       DAVGJL010000018        21865 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.018, whole genome shotgun sequence.
ACCESSION   DAVGJL010000018 DAVGJL010000000
VERSION     DAVGJL010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21865
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.018"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..424
                     /locus_tag="VVE42_000606"
     CDS             <1..424
                     /locus_tag="VVE42_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU3072852.1"
                     /translation="THQSLYATREGFVDELAKQGYVEGENIEIDLQNAQGEQRNLKTI
                     SQQLAESSDVVLAIARPSAQSLANTTQTTPVIFSAVTDPVSAKLVESREHPGGNVTGT
                     SDQSSDAISTQINLIKKVLLKAKTIGILYTQSEPNSVV"
     gene            446..847
                     /locus_tag="VVE42_000607"
     CDS             446..847
                     /locus_tag="VVE42_000607"
                     /inference="COORDINATES: protein motif:HMM:NF016292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3072853.1"
                     /translation="MKEKGFTVVEKTILDSNNVKAAAESLMAEVDMVFVPTDNIILST
                     METVKQVSIKHKVPVFGGSTEMIAVGGLYNYGTNYEELGRQTARMLIRVLKGEEPENI
                     AVELPEKLELHTNQEMADALGIDISKLEGKE"
     gene            859..1752
                     /locus_tag="VVE42_000608"
     CDS             859..1752
                     /locus_tag="VVE42_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU3072854.1"
                     /translation="MNFVLSSLSEGLLWSIVAIGVYLTFRILDIADMTAEGAFPLGAA
                     VVVSQIQAGANPWLATLLALLAGMVAGLVSGMLHTKMKIPALLTGIVTLTGLYSINIK
                     IMGSVPNLSLGDSATVFKQLASLGLTNEGAVFSLSLVCFLLVCLVLTLLMKTEIGLVL
                     RSTGDNIPMSEANGVNVDTMKIVGYMISNGLIALCGSLFAQNDGFSDVTSGTGTIVVG
                     LSSVIIAEVLIHDLTIGGRLLSIGIGAIVYRLIILNIYEIPNLDQNLVRLFNAILLTL
                     VLFAPELQKRLKIRGLKLRNE"
     gene            1765..2568
                     /locus_tag="VVE42_000609"
     CDS             1765..2568
                     /locus_tag="VVE42_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU3072855.1"
                     /translation="MASLLTLENIHKTFEAGTVNENHVLKGLDLEVEEGDFISVIGGN
                     GAGKSTLMNILAGNLSVDEGDLLLAGKSIKNLSVRKRAKDIARVFQDPKMGTASSLTI
                     EENMAIALRRGQKRGLGWGVKEKDRIQFQEALKELNIGLENRLKVDTQYLSGGQRQAL
                     TLVMAALVKPKLLLLDEHTAALDPKTSQMVMDLTQKIVEHHQWTTLMITHDMNHAIEY
                     GNRLIMLYQGKIVVDVKGEEKKHLTVEDLMHLFQKNSGQSLVSDELVLG"
     gene            complement(2601..3311)
                     /gene="treR"
                     /locus_tag="VVE42_000610"
     CDS             complement(2601..3311)
                     /gene="treR"
                     /locus_tag="VVE42_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEU3072856.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIRGQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYVVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
     gene            3496..5463
                     /gene="treP"
                     /locus_tag="VVE42_000611"
     CDS             3496..5463
                     /gene="treP"
                     /locus_tag="VVE42_000611"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEU3072857.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLRDDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEATKSAAKSNQNVV
                     QGVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTVNRIGYQAQVIPALLAGLSLSYLEIFWRKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKWLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFAYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVIFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKTEGDTNLQAEFVAQEEAEFVNHEPVELTSVEII
                     SPLTGQVKELSQATDPIFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVTVGQQLIRFDMDVIKAAGLVTETPVI
                     ITNQDAYTATIPGTYPTTIQAGASLMVATRI"
     gene            5641..7266
                     /gene="treC"
                     /locus_tag="VVE42_000612"
     CDS             5641..7266
                     /gene="treC"
                     /locus_tag="VVE42_000612"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEU3072858.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTENGIGDFRGIIEKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEEMVCVGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWAPFGDTGKYYLHLFDETQA
                     DLNWRNSNVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQMLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDASENAGFSTGTPWLKAGKSYKYINVENEIQGPIFTFY
                     QDLIRLRKEMPIISEGSYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDVEVSEKILLKPYQTLAIYVN"
     gene            7408..7656
                     /locus_tag="VVE42_000613"
     CDS             7408..7656
                     /locus_tag="VVE42_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEU3072859.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            7863..8657
                     /gene="racE"
                     /locus_tag="VVE42_000614"
     CDS             7863..8657
                     /gene="racE"
                     /locus_tag="VVE42_000614"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEU3072860.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIHVEHVEL"
     gene            8654..9625
                     /locus_tag="VVE42_000615"
     CDS             8654..9625
                     /locus_tag="VVE42_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEU3072861.1"
                     /translation="MTNKIYEYKDDQDWYVGSYSIFGGVNSLSDYKIDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSTYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNAKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGESSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKPSL"
     gene            9601..10122
                     /locus_tag="VVE42_000616"
     CDS             9601..10122
                     /locus_tag="VVE42_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU3072862.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPD
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWMEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            10119..10580
                     /locus_tag="VVE42_000617"
     CDS             10119..10580
                     /locus_tag="VVE42_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU3072863.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDAEGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            10571..11311
                     /gene="xerD"
                     /locus_tag="VVE42_000618"
     CDS             10571..11311
                     /gene="xerD"
                     /locus_tag="VVE42_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEU3072864.1"
                     /translation="MPMRDRISAFLEEKQGLSVNSKQSYKYDLEQFLDMVGDRISETS
                     LKIYQAQLANLKISAQKRKISACNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEIL
                     YLDSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQQRIVT
                     IPTALLSELEPLMGQTYLFERGEKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFIL
                     RQIENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            11311..12039
                     /locus_tag="VVE42_000619"
     CDS             11311..12039
                     /locus_tag="VVE42_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEU3072865.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLEAKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLAFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLIGRDQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            12058..12627
                     /locus_tag="VVE42_000620"
     CDS             12058..12627
                     /locus_tag="VVE42_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEU3072866.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKPQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            12614..13336
                     /locus_tag="VVE42_000621"
     CDS             12614..13336
                     /locus_tag="VVE42_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEU3072867.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVANKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            13336..13578
                     /gene="yidD"
                     /locus_tag="VVE42_000622"
     CDS             13336..13578
                     /gene="yidD"
                     /locus_tag="VVE42_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEU3072868.1"
                     /translation="MKRILIAPVRFYQRFISPVFPPSCRFELTCSNYMIQAIEKHGFK
                     GVLMGLARILRCHPWSKTGKDPVPDHFSLKRNQEGE"
     gene            complement(13803..14768)
                     /locus_tag="VVE42_000623"
     CDS             complement(13803..14768)
                     /locus_tag="VVE42_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU3072869.1"
                     /translation="MKTSLKLYFTALVASFLLLLGACSTNSSTSQTETSSSAPTEVTI
                     KSSLDEVKLSKVPEKIVAFDLGAADTIRALGFEKNIVGMPTKTVPTYLKDLVGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSTKANIES
                     LASAFGETGTQKAKEELAKLDKSIQEVATKNESSDKKALAILLNEGKMAAFGAKSRFS
                     FLYQTLKFKPTDTKFEDSRHGQEVSFESVKEINPDILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
     gene            complement(14864..15616)
                     /locus_tag="VVE42_000624"
     CDS             complement(14864..15616)
                     /locus_tag="VVE42_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3072870.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINMASQYADEIVAFKDGQVFSKGS
                     TDQIMQADLLSQLYEIPITLADINDKKICIYS"
     gene            complement(15613..16569)
                     /locus_tag="VVE42_000625"
     CDS             complement(15613..16569)
                     /locus_tag="VVE42_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEU3072871.1"
                     /translation="MQTKSKHTKLFWILIILTIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQTLTENRFLTPSILGIESFYVLLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSLFFLALQGYLKTLMKQDLVFILLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATALVGPMAFFGFMLANLTYLIVKDYQHKLLFIVAILVGF
                     ISLTLGQALIERVFALEIRISMIIESVGGFLFFILLYRRSRQ"
     gene            complement(16559..17518)
                     /locus_tag="VVE42_000626"
     CDS             complement(16559..17518)
                     /locus_tag="VVE42_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU3072872.1"
                     /translation="MKLSHYLIGLLLLLVFLSISIGTSDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTQKMLFAFVSSIVFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKETSESLGISYS
                     LLEKLALFLVALTTSVTMITVGGLPFLGVIVPNLVRKRYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLVFILLLWRGGRKDAD"
     gene            complement(17941..18642)
                     /gene="galE"
                     /locus_tag="VVE42_000627"
     CDS             complement(17941..18642)
                     /gene="galE"
                     /locus_tag="VVE42_000627"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU3072873.1"
                     /translation="MTKTILVTGGTSYIGSHTVKALLNAGYKVHVLDNLSTGNRAAVD
                     SRASFKELDVYDASALKAYLEENQIDAVLHCAGEIVVSESIENPSKYFTANVAGMNQV
                     LKVLSEVGIQKIMFSSTASLYGNNCIDKPVTEDTLLDPVNPYAETKLMGERMIYWMAN
                     RYDWKYVIFRYFNVAGAEMDASNGLRVKNPTHIIPNINKTALGQNDSLKIFGDDYDTR
                     DGSCIRDYIYVLDLA"
     gene            18912..20282
                     /locus_tag="VVE42_000628"
     CDS             18912..20282
                     /locus_tag="VVE42_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEU3072874.1"
                     /translation="MNKKRTVDLIHGPILPSLLSFTFPILLSNIFQQLYNTADVLIVG
                     RFLGQESLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWIL
                     GALLSILVMLLGFLGLYPLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLR
                     SIGDSLAALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIISQGLSAVLCFYYIRK
                     SVPELLPQFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQFSVNTFGAVIISAQ
                     TAARRIMTFALLPMTAISASMTTFASQNLGAKRPDRIVQGLRIGSRLSISWAVFVCIF
                     LFFASPALVSFLASSTDGYLIENGSLYLQISSAFYPILSLLLIYRNCLQGLGQKILPL
                     VSSFIELIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAIL
                     ATKVQS"
     gene            20658..21614
                     /gene="lytA"
                     /locus_tag="VVE42_000629"
     CDS             20658..21614
                     /gene="lytA"
                     /locus_tag="VVE42_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HEU3072875.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKHDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIADKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK"
BASE COUNT         6187 a         4247 c         5060 g         6371 t
ORIGIN      
        1 tacccaccag tccttatacg ctactagaga aggatttgtg gatgagctgg ccaaacaagg
       61 ctatgttgag ggggaaaata ttgagattga tttgcaaaat gcacagggag aacaaagaaa
      121 tctaaaaacg atttcccagc aactagcaga atccagcgat gtcgttctag ccatcgcaag
      181 gccttctgct cagagcttgg ccaatacgac gcaaacgaca ccagtcatct tttcagctgt
      241 aacagaccca gtcagtgcca agttggttga gtcaagagaa catcctgggg gcaatgtaac
      301 tgggacaagc gatcagtcat cagatgccat ttcaacccaa atcaatttga taaagaaagt
      361 gttgctaaag gctaaaacga ttggaatcct ctatactcag agcgagccca attcggttgt
      421 ttaaaaggat gaagctaagc gacttctgaa agaaaaaggc tttaccgttg ttgaaaaaac
      481 aatcttggac agtaataacg tcaaggcggc agcagagagt ttgatggcag aggtggatat
      541 ggtttttgtc ccaactgata atatcatttt gtcaaccatg gaaacagtta agcaggtttc
      601 tattaaacac aaggttccag tattcggtgg ttcaacagaa atgattgctg ttggtggctt
      661 gtataactac ggaaccaatt atgaagaatt gggaagacag acagctcgca tgctgattcg
      721 tgttttaaaa ggtgaggaac cagaaaatat agcagttgag ttgcctgaaa aactggaatt
      781 gcataccaat caagaaatgg cagatgcatt gggaattgat attagtaagt tagaaggcaa
      841 ggaataagga ggattaagat gaattttgtg ttatctagtt tatcagaagg tttgttgtgg
      901 tcgattgtgg cgattggggt ctacttgact ttccgtattt tggatattgc ggatatgact
      961 gctgagggag cctttcctct gggggcagca gtcgttgtat cacagataca ggcaggggca
     1021 aatccttggc ttgcgacttt acttgctttg ctggcaggta tggtagcagg tcttgtatca
     1081 ggaatgctcc acaccaagat gaaaatccca gctctcttga cagggattgt gaccttgaca
     1141 gggctctatt caatcaatat taaaatcatg ggcagtgtac ccaatctttc cttgggagat
     1201 tctgcaaccg tctttaaaca gttggcgagc ttgggtctga caaatgaagg agctgttttc
     1261 tcacttagtt tggtctgttt tttacttgtt tgtttggtct tgaccctttt gatgaaaaca
     1321 gagattggct tggtcttgcg ttcgactggg gataatatcc cgatgagtga ggccaatgga
     1381 gtcaatgtag acaccatgaa gattgttggt tacatgattt caaacggttt gattgctcta
     1441 tgtggttcct tgtttgccca aaatgatgga ttttcagatg tgacttctgg gacaggaacc
     1501 attgttgttg gtttgagctc ggtgattatt gcggaagtct tgatacacga cttgaccatt
     1561 ggaggccgct tattatccat cggaatcggt gctattgttt accgtttgat tattttaaat
     1621 atctatgaaa ttccaaatct agatcaaaac ttggtacgtc tctttaatgc aatcttgctc
     1681 actttggttt tatttgcacc agaattgcaa aagagattaa agattcgtgg tctgaaattg
     1741 agaaatgaat aggaggagaa agttatggca agtttgttaa cacttgaaaa tattcacaag
     1801 acatttgaag cagggacggt caatgagaac catgtcctca aaggattaga tttagaggtt
     1861 gaagagggag attttatctc tgtgattggt ggaaatggag caggaaaatc cactttgatg
     1921 aatatcttgg ctggcaatct atcggtggac gaaggggacc ttctactggc agggaaatcc
     1981 attaaaaatc tgagtgttag aaagagagcc aaggatattg cccgtgtttt tcaagatcct
     2041 aagatgggaa cagcttctag tttgacgatt gaggagaata tggctattgc cttgagacgt
     2101 gggcaaaaga gagggcttgg ttggggcgtg aaggagaagg atagaatcca gttccaagag
     2161 gccttgaagg agttgaatat tggtcttgaa aaccgcttaa aagtagatac tcaatatctc
     2221 tcaggaggac aaagacaggc tttgacccta gtcatggcag ctctggtgaa acccaaactt
     2281 ttgcttttgg atgaacatac tgcggcactt gacccgaaaa ccagtcaaat ggtgatggac
     2341 ttgacgcaaa agattgtgga gcaccatcag tggacgactt tgatgattac ccatgatatg
     2401 aatcatgcga ttgagtacgg caatcgtctc attatgctct atcaaggaaa gatagtggta
     2461 gatgtcaagg gagaagagaa aaagcatctg acagttgaag accttatgca tctcttccag
     2521 aaaaatagtg gccaaagtct ggtcagtgat gaattagttt tgggataaag aaaaaggctg
     2581 agatctggtg tctcagtctt ttatttcttg cgttttgcaa aatctacaaa tctaaatttc
     2641 tctaacttat ggcgactttc ggtaaactga aattggcgtc cgtcttggag atagactttt
     2701 gatttaatcg aaacgacata agggtctttg ccaatgtcca tgagaatctt gtctcggtcg
     2761 cttgagtggt caatggtgat ttccttctga gcataatcaa taaggagttt gaggccattt
     2821 tctatataag aatagataga ctgctcagca atttggcgag tgagatttgg gatgagttcc
     2881 atatccagat agtctgtatc cagaacggat accagatcat ccaccacacg ctggcggacc
     2941 accttccaaa ccatccgaaa ctctgggaaa ccggttatca gtgaggattt tttatcaata
     3001 ataatcttgt ccagactgac cacgttggtt ttagagcgca gtccaagttc tttaactagt
     3061 tcttggtagc tggttaggtt ggatacaggg aaattgacgg tttcttcttt gacgacttga
     3121 gaaccttgcc ctcttatctt tttgatcaat ccttcctctt ggaggagaga cagggccttt
     3181 cggacggtat cacgactcac ttgatattgc tccataaggt cgtgctcgct aggaaggaaa
     3241 tctccgacag cgtaagtctc gttttgaatg gtttcttgga tttgcttaaa taattgttgg
     3301 tatttcttca ttttgcctgc ttaaataggt ttttagtcta gtttaatgaa cacctgtatc
     3361 tattttacca tacaagtgct agaaaattta caaaaaaaca ccctttttat accaaacaag
     3421 ttgtagacaa gtttggtatc gtttacaata tacttatcaa atcaaacttg ctttgacaag
     3481 tataaggaga atcaaatggg aaaatttgaa caagaagcca aagatctgct tcaggcaatt
     3541 ggaggcaaag aaaatgtgac tgccgtaact cactgtgcga cacggatgcg ctttgtttta
     3601 agagatgata agaaggctaa tgttaaagct atcgagtcaa ttccagctgt taaaggaact
     3661 tttacaaatg caggtcaatt tcaggtaatc attggaaatg acgtgcccat cttttataat
     3721 gattttacag ccgtttcagg tattgagggt gtttccaaag aagcaaccaa gtctgcagct
     3781 aagagtaatc aaaacgtggt tcaaggtgtt atgaccactc tggcggaaat ctttactccg
     3841 attattccag ccttgattgt tggtggattg atcctcggtt tccgtaatgt cttggaaggt
     3901 gtccattggt cgatgttgga tggcaagacc atcacagaat cctctcagtt ttgggcaggg
     3961 gttaatcact tcctctggtt gcctggtgaa gctatcttcc agttcttacc agtagggatt
     4021 acttggtctg tttctcgtaa gatgggaacc agccaaattt tgggaattgt tctcggaatc
     4081 tgtttggtat cgcctcagtt gctcaatgcc tatgcggttg cttcaacgcc agcagctgat
     4141 atcgcggcaa actgggtttg gaattttggt tattttactg ttaatcgtat tggttaccaa
     4201 gcccaagtta tcccagcctt gcttgcaggt ttgagtctgt cttatcttga aattttctgg
     4261 cgcaaacata tcccagaagt catttccatg atttttgtac cttttttgtc attgattcca
     4321 gccttgattt tggctcatac tgttttggga ccaatcggtt ggacaattgg acaaggactt
     4381 tcatcagttg tcttggcagg tttaactggt ccagttaaat ggctcttcgg tgcaattttt
     4441 ggtgccctct acgctccatt tgtcatcaca ggtctgcacc atatgaccaa tgccattgat
     4501 acacaattga ttgcggatgc tggtggcact gccctatggc caatgattgc tctttctaat
     4561 attgctcaag gctcagccgt gtttgcctat tatttcatgc atcgccatga tgagcgtgag
     4621 gctcaggttt cacttcctgc aaccatttca gcctatctcg gtgttacaga accagctctt
     4681 tttggggtta acgtaaaata tatttatcca tttgttgctg ggatgactgg ttcagccctt
     4741 gcaggcatgt tatccgttat ttttaatgta actgcggctt ctattggtat cggtggtttg
     4801 ccaggtattc tctctattca acctcaatac atgctgccat ttgcaggaac tatgctagtt
     4861 gcgattgttg ttccaatgct cttgactttc ttcttccgca aggctggtct ctttacaaaa
     4921 acagagggcg atacgaactt gcaggcagaa ttcgttgctc aagaagaagc agaatttgtg
     4981 aaccatgaac cagtagaact tacttcggta gaaattatca gcccactaac tggccaagtg
     5041 aaagaattga gtcaagcgac ggatcctatt tttgcatcag gtgtcatggg gcaaggtcta
     5101 gtcattgaac caagccaagg tgagttgacc tctccagtta atgggacagt gacggttctt
     5161 ttccctacca agcatgccat cggcattgtc tctgacgagg gagttgaatt gctcatccac
     5221 atcggtatgg atacagtagg tcttgatggc aaaggttttg aaagtcttgt agtccaagga
     5281 gatcacgtta cagttggtca gcaactgatt cgttttgata tggatgtcat taaggctgca
     5341 ggtctggtga cagaaactcc tgttatcatc accaaccaag atgcttatac agcgactatt
     5401 cccggaactt atccgacaac gatccaagct ggagcatctc tcatggtcgc tacacgaatc
     5461 taatactctt cgaaaatctc ttcaaaccac gtcagctcta tctgcaacct caaaatagtg
     5521 ttttgagcaa cctgtggcta gcttcctagt ttgctctttg attttcattg agtataattg
     5581 aaatagcaca aatagggacg gaagactgct tcctcccttt tatcagaaaa ggagaaacag
     5641 atgacacttg ataaaggaaa agtagtctat caaatctatc caaaatctta caaagacacc
     5701 actgaaaacg gtattgggga tttccgtggg attatcgaaa aaattcccta tttagccaaa
     5761 ctaggtgtgg atatggtctg gctcaatcca ttctatccaa gccctcaacg ggataatggt
     5821 tacgatattt cagattatat ggcagtggat cctctttttg gtgatatggc tgattttgag
     5881 gaaatggtgt gtgtcggtaa agagcacaag attgacttta tgctggacat ggtacttaat
     5941 cattgttcga cagagcatga atggtttcag aaagccctag ctggtgataa gtattatcaa
     6001 gactttttct tcatccaaga ccaaccaaca gattggcagt ctaagtttgg tggttctgca
     6061 tgggcgcctt tcggggatac tgggaaatac tatcttcacc tatttgatga gacccaggct
     6121 gaccttaact ggcgcaattc caatgtccgt aaggagcttt tcaaggttgt taatttctgg
     6181 cgcgacaagg gtgtcaaagg tttccgattt gatgtgatca atttgatcgg taaggacgag
     6241 gtttcagtgg actgtcctga aaatgaaggg aagccagctt atacagacaa acccatcgtt
     6301 cataactatt tgcgtatgat gaaccaagcc acttttggat ctgacgatag ctttatgaca
     6361 gttggggaaa tgtcttctac caccatggaa aactgtgtcc tctattcgtc gcctgatcgt
     6421 caggaattat ccatgacctt taattttcat cacctcaagg tggactataa ggatggacaa
     6481 aagtggacct tggctccctt tgattttgaa gagttgaaga gtctttacca tagttggggt
     6541 aaggaaatga gtgataaaga cggttggagt gccctctttt ggaacaatca cgatcaacca
     6601 cgtgccttga accgttttgt cgatattcaa aacttccgca aggaaggagc caccatgcta
     6661 gcagccagca ttcacctgtc acgtgggaca ccttatatct acatgggtga ggaaatcggg
     6721 atgattgacc cagactatga ttctatggct gattatgtgg atgtcgaatc gcttaatgcc
     6781 tatcagatgc tcttagaaga aggaaagagt cagcaagaag ccttccagat tattcaagct
     6841 aagtcgcgtg ataattcacg cattcccatg cagtgggatg cttcggaaaa tgcaggattt
     6901 tcaacaggca ctccttggtt gaaagctggt aaatcctata aatacatcaa tgtagaaaat
     6961 gaaatccagg gtccgatttt taccttctat caagacttga ttcgactgcg taaggaaatg
     7021 ccaatcattt cagaaggaag ttacaaacca gcctttgaag atagcaaaca agtctacgct
     7081 tttgaacgcc agtttgagga tcaaaagtta ctagtgctca ataactttta tgctaaagaa
     7141 gtggaaatcg acttgccagc agtctaccaa aatggacaga ttttgatatc aaactatgaa
     7201 gatgtagaag tgtctgagaa aattctactc aaaccctatc aaacactggc tatttatgta
     7261 aattaaactt agcaaggaga cctctttttc agaaaagcag ggttcttttt gctgtctatt
     7321 ccatccttga aagtgtagaa caaactggtc agggtggcta gtttgtggta taatgaaagg
     7381 gactcaaaaa gaaacaggag aaaaatgatg gatttacttt tagcaattgt attgattgtg
     7441 ctagcttttc taggaggagc tcttggagga atgtacttgg ttcgtaagca aatcgaaaaa
     7501 gaatttgctg acaacccacg tttgaatgct gaagcagttc gtactctttt gagtgcaaat
     7561 ggtcaaaaac caagcgaagc taaggtacaa caagtttacc accaaatcat ccgccaacaa
     7621 aaggcagccc ttgctaacaa taaaaagaaa aaataaataa ggaaaagtct gggatgaaag
     7681 ttccagactt ctcactatgt tggttatggt ttagggatga gactttttcc ttgcattcta
     7741 ttgatatttg attatgatta agagagaggt agttgacatt ctatcaagct gtcggtcagt
     7801 tcgttcagta caaggaatca taatgaacga tcaatcagaa aaaagactag aaagaagact
     7861 gtatggataa tcgaccaatt ggttttttgg attcgggtgt cgggggcttg accgttgtgc
     7921 gcgagctcat gcgccagctt ccccatgaag aaatcgtcta tattggagat tcggcgcggg
     7981 cgccctatgg cccccgtcct gctgagcaaa ttcgtgaata tacttggcag ctggtcaact
     8041 ttctcttgac caaggatgtc aaaatgattg tcattgcttg taacactgcg actgcggtcg
     8101 tctgggaaga aatcaaggct caactagata ttcctgtctt gggtgtaatt ttgccaggag
     8161 cttcggcagc catcaagtcc agtcaaggtg ggaaaatcgg agtgattgga acgcccatga
     8221 cggtacaatc agacatatac cgtcagaaaa tccatgatct ggatcccgac ttacaggtgg
     8281 agagcttggc ctgtcccaag tttgctccct tggttgagtc aggtgccctg tcaaccagtg
     8341 ttaccaagaa ggtggtctat gaaaccctgc gtcccttggt tggaaaggtg gatagcctga
     8401 ttttgggctg tactcattat ccactccttc gccctattat ccaaaatgtg atggggccaa
     8461 aggttcagct catcgatagt ggggcagagt gcgtacggga tatctcagtc ttactcaatt
     8521 attttgaaat caatcgtggt cgcgatgctg gaccactcca tcaccgtttt tacacaacag
     8581 ccagtagcca aagttttgca caaattggtg aagaatggct ggaaaaagag attcatgtgg
     8641 agcatgtaga attatgacaa ataaaattta tgaatataag gatgaccagg actggtatgt
     8701 tgggtcttat agtatttttg gtggcgttaa cagtttgagc gactataaga tagattttcc
     8761 tctgtttgaa ttctccaaaa tatttggaga tgaagagtat ggtttcccgc tttcagttac
     8821 tgttttacgc tatggttcta cctaccgttt gttctccttt gtggtagaca tgcttaatca
     8881 agaaatggga cgaaacttgg aagttattca acgtcatggg gccctgctct tggttgaaaa
     8941 tgggcaactc ttgtatgtag aattgcctaa agaaggggtc aatgttcatg atttctttga
     9001 gacaagcaag gtcagagaaa ccttgttgat tgcgactcgt aacgaaggca aaaccaagga
     9061 attccgagct atctttgata agttaggcta cgatgtggaa aatcttaatg actaccctga
     9121 cctgcctgaa gtagcagaaa caggtatgac ctttgaagaa aatgcccgcc ttaaggcaga
     9181 aaccatttct caattaacgg gcaagatggt tttggcagat gattctggtc tcaaagtcga
     9241 tgtccttggt ggcttaccag gcgtctggtc agctcgtttc gcaggtgtgg gagcaactga
     9301 ccgtgaaaat aatgccaaac tcttgcacga attggccatg gtctttgaac tcaaggaccg
     9361 ctcggctcag ttccacacaa ccctagtcgt agccagccca aataaggaaa gtttagttgt
     9421 tgaagcagac tggtcaggtt atattaactt tgaacctaag ggtgaaaatg gctttggcta
     9481 tgatcccctc ttccttgtag gagagacagg tgagtcatca gctgaattaa ccctggaaga
     9541 aaaaaatagt caatctcacc gtgccttagc cgttaagaaa cttttggagg tatttccatc
     9601 atggcaaagc aaaccatcat tgtaatgagc gattcccatg gcgatagctt gattgtggaa
     9661 gaagtccgtg atcgctatgt gggcaaagtc gatgccgttt ttcataacgg cgattctgaa
     9721 ctacgtccgg attctccact ttgggagggc atccgcgttg ttaaagggaa catggacttc
     9781 tacgccggct acccagaacg tctggtgact gagcttggtt cgaccaagat tatccaaact
     9841 catggtcact tgtttgacat caatttcaac tttcaaaagt tggactactg ggctcaggag
     9901 gaagaggccg ctatctgcct ctatggtcac ttgcatgtgc caagtgcttg gatggaaggc
     9961 aagatcctct ttctaaatcc aggttctatc agccaaccac gaggtaccat cagagaatgt
    10021 ctctatgctc gtgtggagat tgatgatagt tacttcaaag tggacttttt gacacgagat
    10081 cacgaggtgt atccaggttt gtccaaggag tttagccgat gattgccaag gagtttgaga
    10141 ctttcttgtt ggggcaggag gaaacttttt tgacccctgc taaaaatcta gctgtgttga
    10201 ttgataccca caatgcggat catgcgaccc tcttgctcag tcagatgacc tatacccgtg
    10261 ttcccgttgt gacagatgaa aaacagtttg ttgggacgat tggactcaga gatattatgg
    10321 cttatcagat ggagcatgac ttgagccaag aaatcatggc ggatacggat atcgttcata
    10381 tgacaaaaac ggacgtagcg gttgtttcgc ctgatttcac cattacggag gtcttgcaca
    10441 agctagtaga tgagtccttc ttaccggttg tggatgcaga gggtattttc caagggatta
    10501 ttacgcgcaa gtccattctc aaggccgtta atgccctctt gcatgacttt agtaaggaat
    10561 atgagattcg atgccaatga gagacaggat ttcagccttt ttagaggaaa agcagggctt
    10621 gtctgtcaat tccaagcagt cctataagta tgatttggag caatttttag acatggtagg
    10681 tgatcggatt tctgagacca gtctcaagat ttaccaagcc cagctagcca atctaaaaat
    10741 cagcgcccag aagcgaaaga tttcggcctg taaccaattt ctatactttc tctatcaaaa
    10801 aggagaggtg gacagctttt accgcttgga attagccaaa caagctgaaa agaagacgga
    10861 aaagccagag attctatacc tagactcttt ttggcaggaa agcgaccatc cagagggccg
    10921 cttgctagcg ctcttaatcc tagaaatggg gctcttgccc agtgagattt tagccatcaa
    10981 ggttgcggac atcaatctgg attttcaggt gttgcgaatc agcaaggctt cccaacagag
    11041 gattgtcacc attcccacgg ccttgctttc agaattggaa cccttgatgg ggcagaccta
    11101 tctttttgaa agaggagaga aaccctattc tcgtcagtgg gcctttcgtc agttagaatc
    11161 ttttgtcaag gagaaaggtt ttccatcctt atcagctcaa gtcttacgtg aacagtttat
    11221 tctaagacaa atagaaaaca aggtcgattt gtacgaaatt gcaaaaaaat taggattaaa
    11281 aacagtcctg accttagaaa aatatagata atggatatta aattaaaaga ttttgaagga
    11341 cccctggact tgctcttgca tctggtttct aagtaccaga tggatatcta cgatgtgccc
    11401 attacggaag tcatcgaaca gtatctagcc tatgtctcaa ccctgcaggc catgcgtctg
    11461 gaagtgacgg gtgagtacat ggtcatggct agtcagctca tgctgattaa gagtcgtaaa
    11521 ctccttccga aggtagcaga agtgacagac ttgggggatg acctggagca ggacctcctc
    11581 tctcaaatcg aagaatatcg caagttcaag ctcttgggtg agcacttgga agccaagcac
    11641 caagaacgag cccagtatta ttccaaagcg ccgacagagt tgatttacga agatgcggag
    11701 cttgtgcatg acaagacgac cattgacctc tttttggctt tttcaaatat cctagccaag
    11761 aaaaaagagg agtttgcaca aaatcacacg actatcttgc gggatgagta taagattgag
    11821 gacatgatga ttatcgtgaa agagtccttg attggacgag atcaattgcg cttgcaggat
    11881 ttgttcaagg aagcccagaa tgtccaagag gtcatcaccc tctttttggc aaccctagag
    11941 ttaatcaaaa cccaggagct gatcctcgtg caagaggaga gttttggaga tatctatctc
    12001 atggaaaaga aggaagaaag tcaagtgcct caaagctaga cttgatagag aggaaagatg
    12061 agtactttag caaaaataga agcgctcttg tttgtagcgg gtgaagatgg gattcgggtc
    12121 cgccagttag ctgaactcct ctctctgcca ccgacaggca tccagcagag tttaggaaaa
    12181 ttagcccaga agtatgaaaa ggacccagat tccagtttgg ctttgattga gacaagtggt
    12241 gcttatagat tggtgaccaa gcctcaattt gcagagattt tgaaggaata ctctaaggcg
    12301 cctatcaacc agagcttgtc tcgggctgcc cttgagacct tgtccattat tgcctacaaa
    12361 cagccgatta cgcggataga aattgatgcc atccgtgggg ttaactcgag tggagccttg
    12421 gcaaagttgc aagcttttga cctgataaag gaagacggga aaaaggaagt attggggcgc
    12481 cccaacctct atgtgactac ggattatttc ctagattaca tggggataaa ccatttagaa
    12541 gaattaccag tgattgatga gcttgagatt caagcccaag aaagccaatt atttggtgaa
    12601 aggatagaag aagatgagaa tcaataagta tattgcccac gcaggtgtgg ccagtaggag
    12661 aaaagcagaa gagctgatca agcaaggctt ggtgacggtt aacggccaag tggtgcgtga
    12721 actagcaacc actatcaagt caggcgacaa ggtcgaagtt gaaggtcaac ctatctacaa
    12781 cgaagaaaag gtctactatc tgcttaacaa accacgcggt gtgatttcca gtgtgacaga
    12841 tgataagggt cgcaagacgg ttgtcgacct cttgcccaat gtcaaagagc gtatttaccc
    12901 tgtgggtcgt ttggactggg atacatcagg tgtcttgatt ttgaccaatg atggggactt
    12961 tacagacgag atgattcacc ctcgtaatga gattgacaag gtttatgtcg cgcgtgttaa
    13021 aggtgtggcc aataaggaca atctccgccc cttgacccgt ggtcttgaga ttgatggtaa
    13081 gaaaaccaag ccagctgttt atgaaattct caaagtggac ccagtcaaaa atcgctctgt
    13141 ggtgcagttg accatccatg aagggcgtaa ccatcaggtt aaaaagatgt ttgaagctgt
    13201 tggtctccaa gtagataagt tgtctcggac tcgtttcgga cacctagact tgacaggact
    13261 ccgtccagga gaatcccgtc gtcttaataa aaaagaaatc agccaactac acaccatggc
    13321 tgtaactaag aaataatgaa acgaatttta atagcgcctg tgcgctttta ccaacgtttt
    13381 atctcaccag tctttccacc ctcttgtcgc tttgagctga cttgttccaa ctacatgatt
    13441 caggctattg aaaaacatgg ttttaagggg gtattgatgg gcttggctcg gattttacgt
    13501 tgtcatccct ggtcgaaaac aggtaaggat cccgttccag accacttttc ccttaaacga
    13561 aatcaagaag gggaatgagg tggggtaaat agatttcaaa atgataaaaa cgcatcctat
    13621 caggtttgag tgaacttgat aggatgcgtt ttagaatgtc aaaattttat actcttcgaa
    13681 aatctcttca aaccgcgtca gctttcatct gcaacctcaa aacagtgttt tgagcaacct
    13741 gcggctagtt tcctagtttg ctctttgatt ttcattgagt attaaattga gtttgaagtg
    13801 gcttatttca aagctttttg tatgtcttca atcatgagtt ttgttgattc aagtccgcct
    13861 ccgcttagat accagaggtc tggtgttagt tggataatct taccattttt agcagcaggt
    13921 gtttcagcga taagggcatt ttctaggaca ccgtcgttgc tagagttgtc cccaccgatg
    13981 gcaagggtac ggttgatgac aaagaggatg tcagggttga tttctttgac actttcaaag
    14041 ctgacttctt gtccgtggcg tgagtcttca aattttgtat cagttggttt gaatttcaag
    14101 gtttggtaca agaaagagaa acgagatttg gcaccaaagg ctgccatttt tccttcatta
    14161 aggaggatcg caagggcttt tttgtcagag ctttcatttt tagtagcgac ttcttggatg
    14221 ctcttgtcta gcttggccaa ttcttccttg gctttttgtg taccagtttc gccgaaggca
    14281 cttgctaagg attcgatatt agccttggta gaagtccagt agtcgtcctt gcttgcttgg
    14341 aagagaacgg ttggggcgat ttctttgaat ttgtctacga atttttgtgt acgtggtgaa
    14401 gcgataatca aatcaggctc aagggcggcg atagcttcta aatcaggttc tttcatagaa
    14461 ccaacatttt tgacagttcc cactaggtct tttagataag tcggaacagt ttttgtaggc
    14521 attccgacga tatttttttc aaatcctaaa gcgcgaatag tatccgcagc gccgaggtca
    14581 aaggccacaa tcttttcagg aactttggaa agtttgacct cgtccagtga acttttaatg
    14641 gttacctctg ttggagcaga gctactggtc tctgtctgac tagtgcttga gtttgtacta
    14701 catgcaccaa gtaggagcaa gaagctggcc actagggcag tgaaataaag tttaagggat
    14761 gttttcataa tttctccttt ttaaaatgtg ataacgattt agggagtctc ttgatcttat
    14821 tgattaagag actgaaggtt ctctaacttg agcttttatg ttactagcta tagatacaga
    14881 tctttttgtc attgatatca gctagcgtga tgggaatctc ataaagttgg ctgagcaggt
    14941 cagcctgcat gatttgatcg gtgcttccct tgctaaagac ctgaccgtcc ttgaaggcga
    15001 caatttcatc tgcatactga ctggccatgt tgatatcgtg gaggacgatg ataatggtct
    15061 tgccgagttc ctccaccagt cgtcgaagaa tctgcatcat gctgacgctt tgcttgatat
    15121 cgagattgtt gagtggttcg tccagcaaga taaagtccgt atcctgggcc agtaccatag
    15181 cgataaagac gcgctggagt tgcccccctg acaggctatt gatgtagcgg tcttttaagt
    15241 tggtcagttc caaatagttc agagtttctc ggattttttc ccagtcttct gatctaagtc
    15301 gacctcggct gtagggaaaa cgtccaaaac tgaccagttc ttcaacagtc aatttggctt
    15361 ggtaattgat tttctgtttt aggatggtta gttcttgggc cagttcttgc gaattccagc
    15421 tctcgatttc acgtcctttg atactgagaa ctccctgatc tttcttggtt agtctgctca
    15481 tgatggagag gagagtcgat tttccagcac catttggacc aataaaggct gtcagttttt
    15541 gaggactgac ttcaagcgaa atgccttgca aaatatcctg tttttgaatg gatttgtcaa
    15601 tgttttccag tttcactgac gagacctcct atatagtaag ataaagaata agaagccacc
    15661 cacactctca atgatcatac tgatacgaat ttccagtgca aagactcgtt caatcaaggc
    15721 ttgccccaag gttaagctaa taaatccaac cagaatggcc actataaaga gtaacttgtg
    15781 ctgatagtct ttgacaatca ggtaggtgag gttggccagc ataaagccga agaaggccat
    15841 aggtcctacc aaggcagtgg ccgttgaggt caaaagcacg attccccaga ggagttcttt
    15901 ctgttctttt tcaacatcga gtcccaatat ctgagccgtt tctctttgca ggtgcaagac
    15961 atctagaacg actgcttttc gaaagaaaaa gattgtcaaa gcgaggatga tcagagaacc
    16021 gatggctagg atggaagtgt tgagatgttg aaaggaggca aaaagactat tttgcagttt
    16081 atcgtattcg tttggatcca ttaggacttg aaggaaggtg ctgatatttc gaaagagact
    16141 tctgagcgct agacagatca gcaggatgaa gaccaggtct tgcttcatca gtgtcttcaa
    16201 gtaaccttgt aaggcgagaa agaagaggga ctggacaaga agtaagacta ggaattctaa
    16261 gataggggat ttgccaagtt gaagaaactt gctttcaaaa accagtagta gggtttgtag
    16321 taggacgtag aaggattcaa ttcccaaaat actaggcgtc aggaagcgat tttccgtcag
    16381 ggtttgaaaa ctaatggtcg aaatcccagt cgcgatggct accaagagat aaacgatgat
    16441 cttttgggaa cgcaacttcc aagcaaaggc tgacaagtga gtgatgggcc aaaagtagag
    16501 aagacaagct ccgatggtaa gaataatgag aatccagaag agcttggtat gtttgctttt
    16561 agtctgcatc ttttcgtccc cctctccaga gaagtaggat aaagacgaga ctaccgatga
    16621 ttcctagcaa gagactgaca gacaactcat agggcctaat cagaactcga gataggatat
    16681 cgcaagccag aactagattg gcaccaacca gtgcgaccat gagtttggtt tgacttaaat
    16741 tatctccata gcgcttgcga acaagattgg gaacgataac tccgagaaat ggtaagccac
    16801 ccacggtaat catggtgacg cttgtcgtta gcgccaccag aaagagggcc agtttttcaa
    16861 gtagggagta ggaaatcccc aaactctcgc tggtttcttt ccctagattc atgatggtga
    16921 aggtttggga taatttccaa acggttatca ggatgatgag gcctaagaag agccactcat
    16981 actgatgggt ctgaatcatg gagaaggagc cctgggtcca ggcagtcata ctctgaacca
    17041 gattgaaacg ataggcgata acttctgtga ctgagccgat aatcccgcta tagatgatcc
    17101 caatcagagg caacatccac ctttccttta cagtaaaaat ggtcataaag gctaggaaga
    17161 agagggtgaa tacgatggat gaaacaaaag cgaagagcat cttttgggtc agactagccg
    17221 atggaaagac aaaaaggctc agcaccattc ccagtttggc ggcttcagtc gttccaactg
    17281 tactcggtgc agcaaactga ttttgggtaa tagtctgcat gagaaggcct gccatactca
    17341 tactagaggc agtcaggaga atactgatag ttcttgggag acgggactct tgaaagagga
    17401 gccaggtctg ctggtcgaaa tcaaatagct ttccccatga aaaatcactg gtcccaatgc
    17461 taatagagag aaagactagg agtagaagta agccaattaa ataatgagaa agtttcatac
    17521 cccgtccttt catgtagatt tggtatcgaa agatacctgc agatataaat gtaacattgt
    17581 ttttctaatc tgtcaataaa atttctgaca atttaataaa taaaacaagg agagagcaac
    17641 aagactttct cctttgttat cctattctaa aatgttttta ccttaatctg ataaaataat
    17701 atcttcgagg gagtagctag ccgtccaatc aagatattgt ttagcttttg aagcctctgc
    17761 taggacactg gctgggtcac tagcacgtcg agcaacaatc tcgtgtggga ttttttaatt
    17821 tagtaattct tcagcagttt taaagatttc tttgatagta tagccttttt tagttcctaa
    17881 gttaaagatt tgagaagaac tgtcttcttg aaataggtag ttcattcctt taacatgagc
    17941 ctatgcaagg tccaagacat aaatgtaatc tcgaatacat gaaccgtcac gtgtatcgta
    18001 gtcatctcca aatattttta agctatcatt ttgtcccaat gcggtcttgt tgatatttgg
    18061 aatgatgtga gttggatttt tcacacgcag accgtttgaa gcatccattt cagccccagc
    18121 aacattaaag taacggaaaa taacatattt ccagtcgtag cgattggcca tccagtaaat
    18181 cattcgttcg cccatcagtt ttgtctctgc ataagggttg acagggtcga gcagggtatc
    18241 ttcagtcacc ggcttgtcaa tacagttatt tccatagaga gaagcagtcg aagagaacat
    18301 aattttttga atgccaactt cagataagac tttgagaact tggttcatac cagcaacgtt
    18361 ggcagtgaag tatttacttg gattttcaat actttcgctc acaacaattt cacctgcaca
    18421 atggaggaca gcatcaatct gattttcttc taagtaagcc tttaaggcgc tagcatcata
    18481 aacatccagt tctttaaagc tagcacgact atccacagcc gctcgatttc ctgtggagag
    18541 attatcgagg acatgcacct tatagccagc atttaaaaga gctttaacgg tatgggagcc
    18601 aatgtagcta gtcccacctg taacaagaat tgttttggtc atgatttttt ccttttctag
    18661 ttttgtacta tagtaaattg aaataagata tgaacaactc tattaggaaa gtcaaattaa
    18721 tttctagaaa tgttttagca gctacagcgt actattccaa actcaaccaa ctataataga
    18781 tcgaaactag aatagtacac atctacttct aaaacattgt taaaaatcga tttgactgtc
    18841 cttatttcat tccgctatat ataagtttgc tgtttcttgt cgctcctctg gaaagctgat
    18901 ataatagctt tatgaataaa aaacgaacag tggacctgat acatggtccg attcttccct
    18961 cgctcttaag cttcaccttt ccaatcttgc tatcaaatat ttttcaacag ctctataaca
    19021 ctgctgatgt cttgattgtt ggacgatttc ttggtcaaga atccttggct gcagtaggag
    19081 cgacgacagc gatttttgac ctgattgtag gttttacact tggtgttggc aatggcatgg
    19141 ggattgtcat tgctcgttat tatggggctc ggaatttcac taaaatcaag gaagcagtag
    19201 cagccacctg gattttaggt gctcttttga gcattctagt tatgttgctg ggctttcttg
    19261 gcttgtatcc tctcttgcaa tacttagata ctcctgcaga aattcttcct caatcttatc
    19321 aatatatttc tatgattgtg acctgtgtag gtgtcagctt tgcttataat ctttttgcag
    19381 gcttgttgcg gtctattggt gacagtctag cagccctggg atttctgatt ttctctgcct
    19441 tggttaatgt ggttctggat ctctatttta ttacgcaatt gcatctggga gttcaatccg
    19501 caggacttgc taccattatt tcgcaaggtt tatcagcggt tctctgcttt tattatattc
    19561 gtaaaagtgt gccagaactc ttgccacagt ttaaacattt caaatgggac aaaagcttgt
    19621 acgcggatct cttggagcaa ggtttggcta tgggcttgat gagttcaatt gtatctatcg
    19681 gcagtgtgat tttacagttt tctgttaata catttggtgc agtgattatt agtgcccaga
    19741 cggcagctcg acgcattatg acctttgccc ttcttcctat gaccgctatt tctgcatcaa
    19801 tgacgacctt tgcttctcag aatctaggag ctaagcgacc tgaccgcatt gttcaaggtc
    19861 ttcgaatcgg cagtcgttta agtatatcct gggcagtttt tgtttgtatt ttcctctttt
    19921 ttgccagtcc agccttggtt tccttcttgg ctagttcgac agatggttac ttgatagaaa
    19981 atggaagtct ctatctgcaa atcagttcag ccttttatcc cattttgagc ctcttgttga
    20041 tttatcgcaa ttgcttgcag ggcttggggc agaagatcct tcctctagtt tctagcttta
    20101 ttgaactaat cggaaaaatc gtttttgtgg ttttgattat tccttgggca ggatataagg
    20161 gtgttatcct ttgtgaacct cttatctggg ttgccatgac agttcaactg tacttctcac
    20221 tattccgtca tcccttgata aaagaaggaa aggcaatttt ggcaaccaaa gtgcaatcct
    20281 agttggattt actgaataaa atccatttcc tctagtgaac atcgaaaaaa cttgtgttct
    20341 cttctttagt ttggtgttga aaatagttta acagactttt gacttctttt atatgatata
    20401 ataaagtata gtatttatga aaaggacata tagagactgt aaaaatatac ttttgaaaag
    20461 ctttttagtc tggggtgtta ttgtagatag aatgcagacc ttgtcagtcc tatttacagt
    20521 gtcaaaatag tgcgttttga ggttctatct acaagcctaa tcgtgactaa gattgtcttc
    20581 tttgtaaggt agaaataaag gagtttctgg ttctggattg taaaaaatga gttgttttaa
    20641 ttgataagga gtagaatatg gaaattaatg tgagtaaatt aagaacagat ttgcctcaag
    20701 tcggcgtgca accatatagg caagtacacg cacactcaac tgggaatccg cattcaaccg
    20761 tacagaatga agcggattat cactggcgga aagacccaga attaggtttt ttctcgcaca
    20821 ttgttgggaa cggttgcatc atgcaggtag gacctgttga taatggtgcc tgggacgttg
    20881 ggggcggttg gaatgctgag acctatgcag cggttgaact tattgaaagc cattcaacca
    20941 aagaagagtt catgacggac taccgccttt atatcgaact cttacgcaat ctagcagatg
    21001 aagcaggttt gccgaaaacg cttgatacag ggagtttagc tggaattaaa acgcacgagt
    21061 attgcacgaa taaccaacca aacaaccact cagaccacgt tgacccttat ccatatcttg
    21121 ctaaatgggg cattagccgt gagcagttta agcatgatat tgagaacggc ttgacgattg
    21181 aaacaggctg gcagaagaat gacactggct actggtacgt acattcagac ggctcttatc
    21241 caaaagacaa gtttgagaaa atcaatggca cttggtacta ctttgacagt tcaggctata
    21301 tgcttgcaga ccgctggagg aagcacacag acggcaactg gtactggttc gacaactcag
    21361 gcgaaatggc tacaggctgg aagaaaatcg ctgataagtg gtactatttc aacgaagaag
    21421 gtgccatgaa gacaggctgg gtcaagtaca aggacacttg gtactactta gacgctaaag
    21481 aaggcgccat ggtatcaaat gcctttatcc agtcagcgga cggaacaggc tggtactacc
    21541 tcaaaccaga cggaacactg gcagacaagc cagaattcac agtagagcca gatggcttga
    21601 ttacagtaaa ataataatgg aatgtctttc aaatcagaac agcgcatatt attaggtctt
    21661 gaaaaagctt aatagtatgc gttttcttgt ggagatattt tcttcaattt ttgttactat
    21721 attaaacaaa aatcaaaaag caaactagaa agttatgctc aaataaaatc taaatttgac
    21781 aatgtaaacc gagtcggata gctttaagta ctgttttgag gttgaagata cgatttttga
    21841 taggaactca tcaaataggt ttaat
//
LOCUS       DAVGJL010000019        20263 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.019, whole genome shotgun sequence.
ACCESSION   DAVGJL010000019 DAVGJL010000000
VERSION     DAVGJL010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20263
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.019"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            1..1133
                     /locus_tag="VVE42_000630"
                     /pseudo
     CDS             1..1133
                     /locus_tag="VVE42_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1232..2854)
                     /gene="groL"
                     /locus_tag="VVE42_000631"
     CDS             complement(1232..2854)
                     /gene="groL"
                     /locus_tag="VVE42_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEU3072876.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNVIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISHRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALANVIPAVATLELTGDEATGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            complement(2870..3154)
                     /gene="groES"
                     /locus_tag="VVE42_000632"
     CDS             complement(2870..3154)
                     /gene="groES"
                     /locus_tag="VVE42_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEU3072877.1"
                     /translation="MLKPLGDRVVLKVEEKEQTVGGFVLAGSAQEKTKTAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDKKYIIVGEANILAIIEE"
     gene            complement(3310..3705)
                     /locus_tag="VVE42_000633"
     CDS             complement(3310..3705)
                     /locus_tag="VVE42_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEU3072878.1"
                     /translation="MYNKVILIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGER
                     EVDFVNMVLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLL
                     ESRAQRAMRENNAGQDLADLVLEEEELPF"
     gene            complement(3783..4544)
                     /locus_tag="VVE42_000634"
     CDS             complement(3783..4544)
                     /locus_tag="VVE42_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU3072879.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEALKA
                     EFAETFPNQTVWTFPLDVTDMVMVKTVCSDILETIGRIDILVNNAGLALGLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTRCFLPAMVKANQGHIINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIEALQAQDI
                     ADTVVYVTSQPRRVQITDMTIMANQQATGFMVHKK"
     gene            complement(4586..5212)
                     /locus_tag="VVE42_000635"
     CDS             complement(4586..5212)
                     /locus_tag="VVE42_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEU3072880.1"
                     /translation="MIFTYNKEHVGDVLMVIVKNSGDAKLDVERKGKVARVFLKDNGE
                     TVAWNIFEVSSLFEIAERGQVFLTDEQVARLNQELQAEGFTEEIVNDKEPKFVVGEIV
                     EMVAHPDSDHLNICQVAVASDKTVQIVAGAPNARVGLKTIVALPGAMMPKGNLIFPGE
                     LRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDSAKHWTA"
     gene            complement(5228..5545)
                     /locus_tag="VVE42_000636"
     CDS             complement(5228..5545)
                     /locus_tag="VVE42_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEU3072881.1"
                     /translation="MIQPASLEELASLVEKAGKKVFLFVADWCGDCRYIYPALPEIEE
                     TNPEFTFIRVDRDQYIDLAKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKQQINDFLA
                     GLK"
     gene            complement(5542..5841)
                     /locus_tag="VVE42_000637"
     CDS             complement(5542..5841)
                     /locus_tag="VVE42_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEU3072882.1"
                     /translation="MNGMKAKKMWMAGLALLGIGSLALATKKVADDRKLMKTQEELTE
                     IVRDHFSDMGEIATLYVQVYESSLESLVGGVIFEDGRHYTFVYENEDLVYEEEVL"
     gene            complement(6039..6227)
                     /locus_tag="VVE42_000638"
     CDS             complement(6039..6227)
                     /locus_tag="VVE42_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein A"
                     /protein_id="HEU3072883.1"
                     /translation="MKASTWVYDKGEWYYVSSSGAMLANDWVKDNGKWYYLASSGKML
                     RNTYTPDGYYVDNSGAWQ"
     gene            complement(6478..7152)
                     /locus_tag="VVE42_000639"
     CDS             complement(6478..7152)
                     /locus_tag="VVE42_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3072884.1"
                     /translation="MKKKAFGIVLLVLAAWILLQGNFGIPSLDGKIWPLLGIVFFAYK
                     SIESILRRHLTSAVFTGLLAIIIANYAYDLLPVTNHSLIWASILVVLGVGYLTHSSKF
                     WNEKKWWYNGKKTVVTDKEVAFGSGTFYKQDQDLVDDQVEVAFGDAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWRVDLKVETSFGAAKADAPVAPTSKTLIIRGDVAFGKL
                     ELVYVK"
     gene            complement(7158..7604)
                     /locus_tag="VVE42_000640"
     CDS             complement(7158..7604)
                     /locus_tag="VVE42_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU3072885.1"
                     /translation="MKLRIEIDGNLEETEIVIKTPTLTDEIADLQRLLQESKAPRLTF
                     YKGTGEYYLDLSEILFFETEGSKIYAHNQKEAYEVRLKLYELESILPRYFNRVSKSTI
                     ANIRQIYSVDKSFSGTGTISFYQTHKEVHVSRYYQSLLKENLRNMR"
     gene            complement(7718..8221)
                     /locus_tag="VVE42_000641"
     CDS             complement(7718..8221)
                     /locus_tag="VVE42_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU3072886.1"
                     /translation="MKNYQEWYRNISSRLTSRPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVYVLIPASGFVALSLFRKKINHPRPYETWDICPLLDKDSSGRSM
                     PSRHVFSATIISMACLHASLPIGLVCLIFSALLGLVRVLGGVHYPKDVLVGYACGLVW
                     GFLFFLF"
     gene            complement(8218..8580)
                     /locus_tag="VVE42_000642"
     CDS             complement(8218..8580)
                     /locus_tag="VVE42_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HEU3072887.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGKAESVHQVTSWLTGYEVS
                     DVLACLDRDVTYGDFFRQAPYYVPERIAITGKICGVRIEEIDDPLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            complement(8646..10505)
                     /locus_tag="VVE42_000643"
                     /pseudo
     CDS             complement(8646..10505)
                     /locus_tag="VVE42_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850459.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            complement(10544..10993)
                     /locus_tag="VVE42_000644"
     CDS             complement(10544..10993)
                     /locus_tag="VVE42_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEU3072888.1"
                     /translation="MDKLMLVFKRFGHQIHLMVQKEAKRCGIEFMGGPQGQVVRFLDN
                     REKNQDLVLIKDIEQELNITKPVASNLVKRIVQNGLVELEASPVDKRAKFVRLTDKAR
                     SQMQQVKAFFERIDKQLMEDIDEDELLIFEKVLGQLQAKYQGNRRRE"
     gene            complement(11117..11833)
                     /locus_tag="VVE42_000645"
     CDS             complement(11117..11833)
                     /locus_tag="VVE42_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU3072889.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHIIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
     gene            complement(12714..14129)
                     /gene="ply"
                     /locus_tag="VVE42_000646"
     CDS             complement(12714..14129)
                     /gene="ply"
                     /locus_tag="VVE42_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HEU3072890.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQ
                     LKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQT
                     EWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTS
                     FLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWNELSYDHQGKEVL
                     TPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRK
                     RTISIWGTTLYPQVEDKVEND"
     gene            complement(14140..14472)
                     /locus_tag="VVE42_000647"
     CDS             complement(14140..14472)
                     /locus_tag="VVE42_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3072891.1"
                     /translation="MYICIDNSMTKNKELVKNQTFKPALLTRRLVKNFMIEEPDLARN
                     PFTRPTLIDLDKVFMLDNTVIPTSYLARRRRNVSEELYEEILDHLVQPRLISLNKSEF
                     MQLNPGTY"
     gene            complement(14543..15151)
                     /locus_tag="VVE42_000648"
     CDS             complement(14543..15151)
                     /locus_tag="VVE42_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3072892.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSATQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRLENPSLLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLINPVYVTLGEKGVLLLD"
     gene            complement(15164..15622)
                     /locus_tag="VVE42_000649"
     CDS             complement(15164..15622)
                     /locus_tag="VVE42_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEU3072893.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKENWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASADAPVKKE"
     gene            15747..16143
                     /locus_tag="VVE42_000650"
                     /pseudo
     CDS             15747..16143
                     /locus_tag="VVE42_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(16884..17144)
                     /locus_tag="VVE42_000651"
     CDS             complement(16884..17144)
                     /locus_tag="VVE42_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3072894.1"
                     /translation="MRERSATGAQGLSKSIKKHLNDITRLTASLLGDEKLSAITSSSA
                     VKADIHRFVIELEPVKSTILQNDDISLDQNEIFEILKNFLDG"
     gene            complement(17203..17754)
                     /locus_tag="VVE42_000652"
     CDS             complement(17203..17754)
                     /locus_tag="VVE42_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3072895.1"
                     /translation="MPANTKVIFQEMFADFQNYYVLIGGTATSIVLDSQGFKSRTTKD
                     YDMVIIYEVKNKEFYTTLNHFLELGEYQGSQKDEKAQLFRFTTTNPEFPSMIELFSIL
                     PEYPLKKDGREIPLHFDQDASLSALLLDEDYYNILVHEKETIQGYSVLSNCGLYSSKI
                     SSNHVSFHLQPQNSVLSSLQLAS"
     gene            complement(17744..17941)
                     /locus_tag="VVE42_000653"
     CDS             complement(17744..17941)
                     /locus_tag="VVE42_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3072896.1"
                     /translation="MLEIWKYRPFVSEFWNDFKNNHDKQFVDPISLYLTLKDDDDPRI
                     EEESEALENMILQYLGEDDAS"
     gene            complement(17945..18529)
                     /locus_tag="VVE42_000654"
     CDS             complement(17945..18529)
                     /locus_tag="VVE42_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3072897.1"
                     /translation="MNVKKIMSIFQSFYVDVSIEELTLTLPISFVKRFEYTQMTFHKE
                     SFLLIKEKRRGSLSSFVTQARTMGEKANMDVVLVFSKLSDSEKKQLLQARVPFVDFKG
                     NLFFPPLGLVLNANDTEIPKELTPSEQLTWIAFLLPDGDIKQIKSVSNLLYGGAYALS
                     HSTFLAETDENTSYVIWQRKFNQLSLPLSQHVLK"
     gene            complement(18937..19362)
                     /locus_tag="VVE42_000655"
     CDS             complement(18937..19362)
                     /locus_tag="VVE42_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3072898.1"
                     /translation="MTSRVFIDADCISAFLWVGTEHLLEKLYSGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVTKGSAEIVSIDIGTEEYALYRDLTRNYDSNKIIGKGEATSIFLA
                     KKHNGILGSNNLRDVKPYVEEFSLEHMTTGDILIEAFKA"
     gene            complement(19355..19849)
                     /locus_tag="VVE42_000656"
     CDS             complement(19355..19849)
                     /locus_tag="VVE42_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3072899.1"
                     /translation="MKKSSVSLILIGEGDETERKADQFASYFLIFPSSLYRMVEEIRE
                     NANRTHLEVEDIIKLGQFYGISHKAMLYRLRNDGYLDAEEIKNMDISVIETASRLGYD
                     TSLYRPLSESKKEMVLGHYIKSTEQLLENNRISQGKYEELLLDAFRYDIVYGLDEEGE
                     LSFD"
     gene            complement(19895..20101)
                     /locus_tag="VVE42_000657"
     CDS             complement(19895..20101)
                     /locus_tag="VVE42_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3072900.1"
                     /translation="MDLSNKASNLRKKLGADGESPIDIFKLVQKIENLTLVFYGLGKN
                     ISGVCYKGTQFSLIAVNSDMPLGR"
     gene            complement(20088..20249)
                     /locus_tag="VVE42_000658"
     CDS             complement(20088..20249)
                     /locus_tag="VVE42_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU3072901.1"
                     /translation="MNMINVCDVGKRIKELRISSNLTQDKIAEYLSLNQSMIAKMEKG
                     ERNITNGFK"
BASE COUNT         6054 a         4534 c         3465 g         6210 t
ORIGIN      
        1 tctaaaattc cttatcttga aacgactggt atgcctacta gaattctcct tagaaagcgt
       61 cgtttcaagt gctatcactg ttcaaaaatg atggtcgctg aaacttctat cgtcaagaag
      121 aatcaccaaa tccctcgtat catcaaccaa aagattgctc aaaagttaat tgaaaagatt
      181 tctatgactg atattgcccg tcagctttcc atctcaactt caactattat tcgtaagctc
      241 aatgattttc actttaaaca tgatttttct tgtcttcctg agattatgtc ttgggatgag
      301 tatgctttta caaaaggaaa gatgagcttc attgcgcaag attttgaaaa gctcaatatc
      361 atcactgttc ttgagggtag aacacaagct gtcatccgaa atcactttct tcgctacgat
      421 agagccgttc gttgtcaagt gaaaatcatt acgatggata tgtttagtcc ttactatgac
      481 ttggctaaac agcttcgctt tcaaatttct aggctcaggc tgaaacagtc tcccagactg
      541 tttcactccc gaatgctaaa atcgtgttgg atcgctttca cattgtacaa catcttagcc
      601 gtgctatgag tcgtgtgcgt gttcaaatta tgaaccaatt ggatcgaaaa tcccatgaat
      661 acaaggctat caagcgctac tggaagctca tacaacagga tagccgtaaa ctgagtgata
      721 agcgatttta tcgtcctact tttcgtatgc atttaaccaa taaagagatt ttagacaagc
      781 ttttgagcta ttcacaagac ttgaaacatc actatcagct ctatcaactc ttgctgtttc
      841 actttcagaa taaggaaccg gagaaatttt tcggactcat tgaggacaat cttaagcaag
      901 ttcatcctat ttttcagact gtctttaaaa ccttcctcaa agataaggaa aagattatca
      961 acgcccttca actacactat tctaacgcca aaccggaagc gaccaataat ctcatcaaac
     1021 ttatcaagcg caatgccttt ggttttcgaa actttgaaaa cttcaaaaaa cggattttta
     1081 tcgctttgaa catcaaaaaa gaaaggacga aatttgtcct ttctcgagct tagctgactt
     1141 caacccacta cagttgacaa agagcctata aaaagaaggg ttagtcattc cctccttttg
     1201 tgttttttat aagttgtttt ctatagaaag cttacatcat cccgcccatc atgcttggat
     1261 ccattgctgg agctggggct actggttctg gtttattggc tacgactgct tctgttgtca
     1321 aaatcaagct ggctacagat gctgcatttt gtagggctga acgactcact ttaactggat
     1381 caatgatacc ttgatcaatc atgttaaccc actcgccagt tgctgcgtta aatcctatac
     1441 caagctcagc atttttcaaa cgatcgataa cgatagatcc ttcaaatcct gcattgtgag
     1501 caatttgacg aacaggttct tccaaagcac ggagaacaat attacgtcct gttgcttcat
     1561 ctcctgtcaa ttccaaggta gcaacagctg gaatcacatt ggcaagagct gttccaccac
     1621 ctgcaacaat accttcttca acagctgcac gagtagcgtt gagggcatct tcaatgcgga
     1681 gtttcatttc tttcaactca gtttcagttg cggctccgac cttaataacc gctacaccac
     1741 ctgacaattt ggccaagcgt tcttgcaatt tttcacggtc aaattcagaa gttgtagttt
     1801 cgatttgaga cttgataacc gcaacacggt gagaaatcgc ttcaggattt cctgcacctt
     1861 ctacaataac cgtgctatct ttgtccacgg tcactctcgc tgcttgacca agagcttcaa
     1921 ttgtcgcatc tttcaactca agaccaaggt cttctgtgat aactgttccg cctgttaaga
     1981 tggcgatatc ttcaagcatg gctttgcgac ggtcaccaaa accaggtgcc ttgactgcta
     2041 ctacgttgaa ggttccacga atcttgttca aaacaagagt tggaagagcc tcaccatcca
     2101 catcatccgc aataatcaag agtggacgat tgctttggag aatgctttcc aaaagtggca
     2161 agatttcttg gatattggaa attttcttgt ctgtaatcaa aatgtacgga ttttcaaggt
     2221 cagccaccat tttttcgcta tctgtcacca tgtactgtga aaggtaacca cggtcaaact
     2281 gcattccttc tacgacttca agctctgttt ccataccacg tgactcttcg atggtgatga
     2341 caccgtcttt gccaactttt tccattgctt cagagatgta ctcaccaact ttttcagaac
     2401 gagaagatac ggctgcaact tgagcgatag cttctttatt ggcaacaggg atgacgttgt
     2461 ttttcaaagc ttcaactgct gcggcaactg ctgtttcaat cccacgacga ataccgattg
     2521 gatttgcacc tgctgtgacg tttttgattc cttcacggac gattgcttgg gtcaaaacag
     2581 ttgcagttgt agttccatca cctgcgatat cattggtttt tgaagctact tctgatacca
     2641 atttggcacc catattttca aaatggtctt ctaattcaat ttctttggca atagtcacac
     2701 cgtcattggt aatcaagggt gaaccgaatg acttttcaag aacgacattg cgaccttttg
     2761 gtcccaaagt tactttaaca gtatctgcaa ggatatcgac accacggacc atagctgaac
     2821 gggcatctga tgaaaattta atttcttttg acatacttac tttctccttc tattcttcaa
     2881 tgattgccaa gatgttagct tcgcctacga tgatatactt tttatcgcca tctttgacat
     2941 caagacctgc gtgggcttca actaagacac ggtctccagt tttaacactt ggagcaacca
     3001 agtcaccgtt caaggtacga acaccttgtc cagtagccac aacttgggct gttttggttt
     3061 tttcttgggc tgaacctgcg agaacaaagc ctccaacagt ttgttctttt tcttctactt
     3121 ttaagaccac acggtcccct aatggtttca acatctgatt tcctccataa tgagatagat
     3181 attattagca ctctttatac ctgagtgcta attcatagtt ctattgtatc acttagtcag
     3241 aaataatcaa gaaaaaagtc tgactttctc aagataaaaa gcctgagacc aactcagact
     3301 ttttaattct taaaatggca attcttcctc ttccaagacc aaatctgcca aatcttggcc
     3361 tgcattattt tcacgcatag cacgttgggc acgactttcc aagagttgga atcctgtcac
     3421 aaggacttcg gttacataat tcatctgacc atttttctca aagcgacggg tacgcaattc
     3481 tccatcaacg gaaatgagac tacctttggt tgcgtagctt gccaaagttt ctgctagcct
     3541 gccccatagg accatattga caaaatcaac ttcacgttca ccgttttggt ctttgtaacg
     3601 acggttgaca gcgatagttg ctcgcgctac tgacttgtca ttgttggttt tgtgcaattc
     3661 tggtgtagac gttaaacgtc caatcaagat aactttatta tacatatttt cttcctccta
     3721 cttatctatt cgtaggaaat caaaaaaagt tacagaaatt tgtaactttt cgagaaaatt
     3781 ttttattttt tatgaaccat gaaacctgtc gcctgttgat tggccataat ggtcatatct
     3841 gtaatctgaa cacgacgagg ttgactagtc acatagacta ctgtatctgc aatatcctga
     3901 gcttgcaaag cttctattcc ttggtaaacg gacgcagctc gttctttatc accatgaaaa
     3961 cgcactgtag aaaaatctgt ttcgacaatt ccaggctgaa tggtcgtcac cttgatatcc
     4021 gttgcgatgg tatcaattcg cagtccatct gaaaaggtct taactgccgc cttggtggct
     4081 gagtaaacag ctgcaccagc ataggcataa attcctgcgg ttgaccccat attgataata
     4141 tgaccttgat tggcttttac cattgctggc aagaaacagc gagtgactgc catcaaacct
     4201 ttgacattgg tatccaacat ggtcagcata tccaactctt catagtcttg atagggagct
     4261 aagccaagag ccagtccggc gttgttgacc aagatatcaa tcctccctat cgtttctaga
     4321 atatcagagc aaacagtctt caccatgacc atatccgtca catctagtgg aaaagtccaa
     4381 actgtttgat ttggaaaagt ttctgcaaac tccgccttaa gagcttctag tctgtctatc
     4441 cgtcgtcctg ttagaacgac atcctcaccc tgctccagat aagcacgcgc aatcgcttca
     4501 ccgattcctg atgtcgctcc tgtaatcaca acattttttg ccatcttatt tccttctagc
     4561 tggtctatca gatattaaca acttcttagg cagtccagtg tttagctgag tcaaatggtg
     4621 ttccgacaac ttggtcttct gataattcaa gcaccccacg tttttgtgga gcatttggca
     4681 gatgcaattc acgaggactg cacatcatac caaaactctt ttcaccacga agttcacctg
     4741 ggaaaatgag attccctttt ggcatcatag ctccaggaag cgcgacaatg gttttcaacc
     4801 ccacacgcgc attgggagct cctgcaacga tttgtacagt cttatcactt gcgactgcaa
     4861 cttggcagat gttgaggtgg tcactatctg gatgggctac catctcaaca atttcaccga
     4921 caacaaactt aggttcctta tcattaacaa tttcttctgt aaaaccttcc gcctgcaact
     4981 cttggttcaa acgagcgact tgctcatctg tcaaaaagac ttgaccgcgc tctgcaattt
     5041 caaataaact tgaaacttcg aaaatattcc aagccactgt ttccccatta tctttgagaa
     5101 aaacacgggc taccttgcct ttgcgctcca catccagttt ggcatctccg ctatttttca
     5161 cgatgaccat aaggacatca ccgacatgtt ctttattata tgtaaaaatc attgtttcct
     5221 ttttctccta tttcagtcct gctaaaaagt cattgatttg ttgcttgctt ttacggtcgc
     5281 gattgacaaa acgaccgatt tccttgtcct tttctagaac aacaaggcta gggattccat
     5341 atacatccca gagtttggcc aaatctatat actggtctct atccactcga ataaaggtaa
     5401 actctggatt agtctcctca atttctggta aggcaggata gatataacga caatcgccac
     5461 accagtctgc cacaaaaagg aagaccttct tgcccgcttt ttccactaaa gatgctaatt
     5521 cttctaaact tgctggctgt atcataagac ttcctcctca tagactaggt cttcattttc
     5581 atagacaaag gtataatgac ggccatcctc aaaaataacg ccaccaacca agctctccag
     5641 actgctttcg taaacttgaa catagagggt cgcaatttcc cccatgtcgg aaaaatggtc
     5701 tcgcacaatc tctgtcaact cttcctgagt cttcatgagc ttacggtcat ctgcaacttt
     5761 tttcgtggca agagcaaggc ttccgatacc tagcagagcc aagcctgcca tccacatttt
     5821 tttagctttc ataccattca ttttaacaca aaaaaggctt caggacaaat gaggaagcag
     5881 cagaaaagca agtaaaaagc ctcttccttt aaggaaaagg acttcttata ctaagagcaa
     5941 actagaaagc tagccgcagg ctgctcaaaa cacgattttg aggttgtaga taaggcgacg
     6001 ttgacgtggt ttgaagagat tttcaaagag tattattctt attgccaggc gcctgagtta
     6061 tcaacgtagt aaccatcagg tgtgtaggta ttgcgaagca tcttacctga tgaagctaga
     6121 taataccact tgccattatc tttgacccaa tcattcgcta gcatggctcc agaagaactt
     6181 acataatacc attctccctt gtcataaacc caagtgcttg ctttcatggc tcctgagcaa
     6241 ttaaaggttc aaaaaactgt ccaatatcat tcattttttt aaagcatttt acactacatc
     6301 atccacgctg taggacatag atacatcaaa cgtaagagcc aaaatatcga aaaatataca
     6361 acttttaaag gcaacatttc attttttaaa gtctaacaac catagaactg aaatcagagc
     6421 aaggcatcaa cttcctactc ttagtacgtc tattttgatg gttgaagtga agatttttta
     6481 tttaacgtag acaagctcca acttcccaaa agccacatct ccacggataa tcaaggtttt
     6541 gctggttggg gctacaggag cgtcagcctt agctgcacca aaggaggttt ctactttcaa
     6601 atctacacgc cagtgttgtg gaacatagac ggttgcattt ccgaaggcca cttcaatatt
     6661 taaagttgca aaatcaccta gcatctctgc attatcatag tagattttag catccccaaa
     6721 agcgacttcc acttggtcat ctacgagatc ttgatcttgc ttatagaagg tcccgctacc
     6781 aaaagcgact tccttatccg tgacgactgt ttttttccca ttgtaccacc attttttttc
     6841 attccagaac ttacttgaat gcgtcagata accaacacca agtaccacca agatgctagc
     6901 ccaaataaga gaatgattgg taactggtaa caagtcataa gcgtaatttg caatgatgat
     6961 cgccagtaaa cctgtaaaaa ctgccgaagt gagatgacgt ctaaggatgg actcaatgga
     7021 cttataagca aaaaaaacaa tacctagtaa aggccatatt ttaccatcca aagaaggaat
     7081 tccaaaattc ccttgcagca agatccaagc tgctaaaacc aataaaacaa taccaaatgc
     7141 tttctttttc atgtttttta cctcatgttt cttagatttt cttttaggag ggattggtaa
     7201 taccgtgaga catgaacctc cttgtgcgtc tgataaaagg aaatggtgcc cgttcctgaa
     7261 aaggacttgt ccactgagta aatctgacgg atgtttgcga tcgttgactt ggaaactcga
     7321 ttaaaatagc gaggcaagat agactccaac tcatagagct tgaggcgaac ctcataagct
     7381 tccttctggt tatgagcgta gatcttgctc ccttctgttt caaagaagag aatttctgac
     7441 aggtctagat aatattcacc tgtccccttg taaaaagtca acctcggagc ctttgactct
     7501 tgcaagagcc gttgcaagtc tgcaatttca tctgtcaaag tgggggtctt gatgacaatt
     7561 tcagtctcct ctaaattgcc gtcaatctca attcgtaact tcatcacatc tcctttctga
     7621 ctctactata tcaaagctaa gttaggaata caagggcaaa aaagcaagtg gtcaatttga
     7681 agcagtaagt ggttgtagga ctagcttaac ttacatgtca gaataagaag aaaaggaatc
     7741 cccacaccaa accacaagca tagccaacca agacatcctt gggataatga acacccccta
     7801 aaaccctcac caaccccagc aaagctgaaa agatcaagca tactaagcca ataggtagac
     7861 tagcatgcag acaggccatg gagatgatag ttgccgaaaa gacatggcga ctgggcatcg
     7921 accgtcccga actatcctta tcaagaaggg gacagatatc ccaagtttca taaggtctcg
     7981 ggtgattgat tttcttacga aacagggaca aggccacaaa acctgatgca ggaataagca
     8041 cataaacccc aacttgctga ccaagtccta gttgcaggta agtggtgact agcaagatca
     8101 gatagaccag gggcatggcg actgtcatca aacgattgaa actgcgtaat aggaataaaa
     8161 gaattggacg actagttagt ctggagctga tatttcgata ccattcttga taatttttca
     8221 tttatgcttc tcatactttt ctagaacttg ttgagatgtt ttccccttgg caagccaatc
     8281 cactagcttg tccaagcgac gtatttcctg catcagagga tcatcaattt cctcaatacg
     8341 gacaccgcaa atcttccctg taattgcaat tcgttcaggg acataatatg gtgcttgtct
     8401 aaaaaaatct ccgtaagtaa catccctatc cagacaagca agaacatcac tcacttcata
     8461 ccctgtcaac caactggtca cttggtgaac tgactctgcc tttccaccct ttcgctcaac
     8521 ttttgcaatc aaggctaaat agacagcagc aaatttcata ttgtataatt tctgactcat
     8581 acttctctct ttctatgtgt actagtataa ataagaaaaa gaaggccgtc aagccttctt
     8641 ttgatttatt cttctgcttc atcttctgta aattgactat tgtacaagtc agcgtagaag
     8701 ccaccttgcg ccatcagttc ctcatagttg ccttgctcga tgatatttcc atctttcatg
     8761 accaagatca agtctgcatt tcggatggtt gacaagcggt gggcaatgac aaaggatgtg
     8821 cgtccttcca tcaaacggtc catggctttt tggatcaatt cctctgtccg tgtgtcaaca
     8881 gaagaagtcg cctcatccaa aatcaaaagc ggtgcatcct taagaagggc acgagcaata
     8941 gtcaatagtt gtttttgtcc tacagacaag gtcacggtgt catccaagat ggtatcatag
     9001 ccatctggca aggtcataat aaagtggtga attcccacag ccttactagc ttccatcatt
     9061 cgttcatcac taatccctat ttgattatag atgagattgt ctcgaatagt tccttcaaag
     9121 agccaggtat cctgcaagac cattgaaaag gcatcatgca cttctgaacg cgtcatagcc
     9181 ttggtatcca caccatcaat gtgaatactt cccttatcaa tctcatagaa tttcatcaaa
     9241 agattgacaa tggttgtctt accagtccca gtcggcccaa caatggcaac cttttgacca
     9301 gcatgagctg tcgcagagaa gtcatagtct tgaacattga caccgtccac cagaatttct
     9361 cctgctgaca cgtcgtagaa acgtggaatc agattgacca gagttgattt accagaacct
     9421 gttgacccaa taaaggccac tgtttgacca gtttctgctt taaagctaac atgttcaata
     9481 actgcctccg aatttgccgc atagcggaag gtcacatcct taaactcgac ctgacctttg
     9541 aagttttcat cagtcagctg cacttgaaca gggttttgga tagaagaatg caaatctaaa
     9601 acttgattaa tccgcttagc agagaccata gttcggggaa gaacgatgaa gagtgctccc
     9661 atgagaagga agcccatgac aacctacatg gcataagaca tgaaaacaat catgtcacta
     9721 aagagaggca gacgcgctat cggagcagcg tcgttaatca cataggcccc aatccagtaa
     9781 atcgccacac tcaaaccact tgaaatcccc atcatgatag gattcaaaat agccataaga
     9841 cggttgacaa acaaattcaa acgggtcaat tcatcattta ctgctgcaaa tttttcattt
     9901 tgataatcct ctgcattgta ggcacgaacg acacgaatac ctgttaaact ctcacgagtg
     9961 atactgttca gtttatctgt cagcccctga atcaaggact gttttggaaa ggctagcgtc
    10021 atcaaaacgg tcgtcatcag gacgttgata atcactgcca caagtacggc ccagagccag
    10081 tattctgaat gacctaaaat cttcccaata gcccagatag ccataattga accacgcgtt
    10141 accacttgca agcccatagt aatcaacatt tgaacttgag taatgtcatt ggtagtacgc
    10201 gtcaagaggc taggaattga aaatttctta atctctgtct gcgagtaatc caaaactcgg
    10261 ttaaaaatat cacttctcag cctactagta taagaagccg ccactcggga tgcaaaaaat
    10321 ccaactgcaa ctacggacaa gaaggcaaga aaggacattc ccatcatcat gcttgccgac
    10381 tgccacaact catctaaatt agtttcttga ctacctagca aatccgtaat tttcgagata
    10441 taggtcggca cttccaactc tagatagacc gaaaagcaag taaagagaat ggctagtaaa
    10501 atcatccccc attcttttct actaattctt ttggctaatt tctttattct ctcctcctat
    10561 tcccttgata ttttgcctgt agttgaccga gaaccttctc aaaaatcagt aattcatctt
    10621 catcaatgtc ttccatcaac tgcttgtcta tgcgttcaaa aaaagcctta acctgttgca
    10681 tctgagaacg tgctttgtcc gtcagacgaa caaacttagc ccgcttatca acaggactcg
    10741 cctccaattc caccaaacca ttttgcacta tacgcttaac cagattacta gcaacaggct
    10801 tggtaatatt gagttcctgc tcgatatctt taatcaagac caagtcttgg tttttctcgc
    10861 gattatccaa aaaacgcaca acctgacctt gcggcccacc cataaattca atgccgcaac
    10921 gtttggcttc cttttgcacc atcaggtgaa tttgatgacc aaaacgctta aagactaaca
    10981 tcagtttatc cataatctcc cccttctaaa taaaaatagt tctctggaga ataattaaat
    11041 ttctatgaga actattttct tgattaaaaa aatcccaagt gattttctca cttaggatca
    11101 tgttctatag gttaaattaa aacccatcta cgttcgtata aatcttttgg acgtcttcgt
    11161 cgtcttcaag aacgctgtaa agtttttcaa aggtttcaag gtcttcgcct gacaattcca
    11221 cttctgactg aggaatcatt tccaattcag tcacttggaa ttcttcaata ccagactcac
    11281 ggagggcaac gatagccttg tgaaggtcag ttggcgctgt gtaaactgtg attgtacctt
    11341 cttgtgcttc tacgtcatcc acatccacat ccgcttcgag caattgctca aagactgcgt
    11401 ccgcatcttc acctccaaat acaataacac ctttgttgtc aaaaaggtaa gaaacagaac
    11461 ctgaagcgcc catgtttccg ccgtttttac caaaggctgc acggacattg gctgctgtac
    11521 ggttaacgtt agaagtcaaa gtatccacaa ttagcataga gccatttggc ccaaaacctt
    11581 cgtaacgtcc ttctgtaaag gtttcgtctg tgtttccttt ggctttatca atcgctttat
    11641 cgataatgtg ttttggcact tgggcttgtt tagcacggtc gataacgaat ttcaaagctg
    11701 agtttgattc tggatctgga tcaccttttt tagctgctac atagatttct acaccaaatt
    11761 ttgcatatac tttagagttg gctccatctt tagccgtttt cttggctacg atattggccc
    11821 atttacgtcc cattaggaat ctcctttttt cacattttaa tctttcttat tataacacaa
    11881 gtttttttga ttttcactag aggaaatgga ttttattagc aaatcaagct aggatagcac
    11941 tttacctgct aagatggtct tacctttcta tctttatcaa cagacactca tccacattca
    12001 aaaaacaaac tagaccatta tctgcaaata gaaagtttca gccaagtttg acaaagtcag
    12061 ctcaaattac tgtttgaagt ttgtagatat aagcgacaaa aacaatcata ctgcaccttt
    12121 tgttgacagt ctactccaga catatcatag ttcaagtaaa tactttgaaa ttcaacagtt
    12181 cttataggcg ctattgtatt ctaagaaatc aatagaagag tttctaagca aacctctaat
    12241 actcaataaa aatcaaagag caaactagaa agctagcctc aggttgctca aaacactgtt
    12301 ttgaggttgc ggatggggct gacatggttt gaagagattt tcgaagagta taatttacgt
    12361 gttcccaaga tggagaagtt agactagtac actggcactt ctaaaccatt gctagcaatt
    12421 gatttgttca tatttaattt cattttttcc ataaatgggt attagatata aacagcaaaa
    12481 tatttccgat acgtgtcgtt cttaaatttc caatcatcta aaacaagtaa aggataatca
    12541 atcccctgta tatcaaggaa ttggctaccc tttttacttt tttacacatt ctgtttgata
    12601 gattcatttt aacatcacga gcatactcca atggaaatcg ctaggcaaga gataaacttt
    12661 cagatatccg cagagagatc atcgcctctt tttgtcgcaa gcattctcct ctcctagtca
    12721 ttttctacct tatcttctac ctgaggatag agagttgttc cccaaataga aatcgtccgc
    12781 ttacgcacta gtggcaaatc ggttttttca taaaccgtac gccaccattc ccaggcaagc
    12841 ccggtacact ctctaatctt gacagagaga ttacgaacat tcccttttaa aggaatacta
    12901 gtggtaaagt gagccgttaa atcctgccca tttctgtccc aagccttagg agtcaagact
    12961 tccttacctt gatgatcata ggataattca ttccaagtaa tataatattg ggcaacatag
    13021 gcaccactat gatccagcag taaatctccg tttctgtaag ctgtaacctt agtttcaaca
    13081 tagtctgtac tattttgaaa ggtcgcaact acattgtcac gtaaaaaaga agttgtatag
    13141 gaaatcggca agcctggatg atctgctgta aagcgactgc cttcttgaat caagtcctct
    13201 accatatcca ccttgcctgt tacaactcgg gcacccgaac ttgggtcgcc ccctaaaata
    13261 accgccttca cttctgtatt gtccaaaatc tgcttccact ctgtctgagg agctaccttg
    13321 actcctttta tcaaagcttc aaaagcagcc tctacttcat cactcttact cgtggtttcc
    13381 aacttgagat agacttggcg cccataagca acactcgaaa tatagaccaa aggacgctct
    13441 gcagaaattc ctctctgttt taaatcctct accgttacag tatcttgaaa cacatctcct
    13501 ggatttttaa cagcatctac actgactgta taataaatct gcttaaaatt aacaatctga
    13561 atctgctttt cgcctgaatg gacagagtta aaatcaatat caagagaatt ccctgtcttt
    13621 tcaaagtcag aaccaaactt gaccttgagt tgttccatgc tgtgagccgt gattttttca
    13681 tactgcattc tagctgggac attattgacc tgaccataat cttgatgcca cttagccaac
    13741 aaatcgttta ccgctccgcg aacacttgaa ttgctggggt cttccacttg gagaaagcta
    13801 tcgctacttg ccaaaccagg caaatcaata ctataagtca tcggagcacg atcgaccgca
    13861 agaagagtag gattattctc taacaaggtc tcatccacta cgagaagtgc tccaggatag
    13921 aggcgactgt cgttggtagc tgttacagaa atatcacttg tatttgtcga caagctccgc
    13981 ttctttcttt cgataacaac aaactcatcg ggtagctgat taccctcttt gatgaaacga
    14041 ttttcaatac tttctccctg atgggtcaag agtttctttt tatcgtaatt catagctagt
    14101 ataaagtcat ttactgcttt atttgccatc ttctacctcc taataagttc ctggattgag
    14161 ttgcataaac tcagacttgt tcagcgaaat cagccgtggt tggactaagt gatccaaaat
    14221 ttcctcgtac aattcttctg agacattgcg tcgccgtctg gctagataag aagtcggaat
    14281 aaccgtatta tccaacataa ataccttatc taagtcaatc aaggttggtc ttgtaaaagg
    14341 attacgagct agatccggct cttctatcat aaagttcttg accaaacgtc tggtcaagag
    14401 agctggtttg aaggtctgat ttttaaccaa ctctttgttt ttagtcatgc tgttgtcaat
    14461 acagatatac atatgattct tcacagccaa atcgctacta atagtcggaa aaggcaaata
    14521 aagagctaca acatctcctc tcttaatcaa gcaagagcac ccccttttct cctaatgtaa
    14581 catagacagg attgatcaag tcttctgatt gactcagaat ttccaaagtt tgagtttggc
    14641 gcgctgtcaa tttagtagca tcttgtctct tcaatacaaa atgcttgtcg ccaataacct
    14701 tgacaatata atccttctcc aaagctgact ggtaaatcca catcagatgt tgtctgtcct
    14761 gagaactcaa gagagaagga ttttcaagcc tcccgatagt ctgataaaaa tcaaaaacag
    14821 gagctaactc ctgccaatct gattggctag ttgtcaaggc taaaaaaagg gctttgcgag
    14881 ctgatacttc ttggttagcc ttgagagtta ctttcccctc caagtttttt agaaatcggg
    14941 aaactccaga aagcaaattt ttctctaact gcgagaaata aaaacctttc gttcccagac
    15001 ataagtcttt catgtcgctt tctctagcaa ataagagctc aaacatttga tagtaaaaga
    15061 aaaatatctg gcactgggtc gcgctcatct tttccttatc ggcttctttt tttaaccaga
    15121 gcaagggcga caggtagctg gattgagaca tttcctctac ctcctactct tttttaactg
    15181 gagcatctgc actagctgcc acttcttttg actggatact ttcccactgg ttaatctcct
    15241 ctgagataag accttcgcat gtcttgacaa atagggcaaa agccttggtc tttcctgcat
    15301 atttctccgt ttggcattga tagaggaatt tttctttctc caggagttgc gcagtttttt
    15361 ggtaagaaat ccaattttcc tttgcattat acaaattgat aatcccctca cacagcaagc
    15421 cgagactgga taaggcaacc gaaatcaaac ggtagcgatc acctggcata ggaatagcac
    15481 aaaagacagc tatgaggaaa cctgccacga tttctgttat ttttaatacc ttatagcgtc
    15541 tacgatgttg aacgcttttc tttaaaaaat gagctatctg tacgtctaat cgctctgtca
    15601 ggtacatttc ttctggcgtc atattcgtaa ctcctttcat ttactttgat aaccatagta
    15661 taacatattt gaatatcaaa gtatagtaaa aactggacta taacttatta gacttcctgc
    15721 gaaacaaaat atggtatagt agttctatga attatgaagc aagtaaacaa ctaactgatg
    15781 cacgatttaa gcgtcttgtt agtgttcagc gtaccacttt tgaagagatg ttagctgtat
    15841 taaaaacagc ttatcaaaaa agtcggacaa gctggtaaaa tcttggctga cagtggttat
    15901 caagggctca tgaagatata tcctcaagca caaactccac gtaaatccag caaactcaag
    15961 ccgctaatag ctgaagataa agcctataac catgcgctat ctaaggagag aagcaaggtt
    16021 gagaacatct ttgccaaagt agaaatgttt aaaatgtttt caacaaccta tcgaaatcat
    16081 cgtaaacgct tcggattacg aatgaatttg attgctggta ttatcaatca tgaactagga
    16141 ttttggtttc gcaggaagtc tattatatat gcacagacat atgaaatctt tttgagacag
    16201 cagttatgat atatttatcc attcacaaca tcttctgtac taatactaca atattggtac
    16261 attttgatta cacaatcgtt tcaaaaaata tgatggaagc ctgagtattt tatagtaatt
    16321 cccttcctat caactagatc aaccagttga gtgttgaatg ttgaaacacc ctagcctcag
    16381 caaaggacta tgatcagcat ttcctagcca ataggacgct ccaaatcacc tattctaagt
    16441 ccatcactag cttggtcagt aagatacaga ctgcaaggcg agactgacgt ggttgatgag
    16501 attttggaag agtattacaa tcatagattc accagtagac ttgaccgcag atttaactat
    16561 agtagattga aactagaata gtacatctct gcttctaaaa cattgttaga aatcgatttg
    16621 actgtcctga tcaatttgtc ctgttctcgt ttcatttcac tatactaaaa aaccacctga
    16681 aacaaatcaa gtgactagag gataaaaaca aagactaggc aaaaagtttt gaaaagcaaa
    16741 aaacgcataa tatcaggtct tgaaaaacct tgatactata cgtttgattg tgggaagatt
    16801 gacttcattt tctcctgaaa ttgaagtttt ttctagtttc cagatataga cgaattcaat
    16861 atagccatct cgctacaatt attttaacca tcgagaaaat ttttcagaat ttcaaaaatt
    16921 tcattttgat ccaatgaaat gtcatcattt tgaagaatag ttgacttcac aggctctaat
    16981 tctatcacaa agcggtgtat gtctgctttt accgcactac ttgatgttat agccgataac
    17041 ttttcatctc ctagcaagga agctgtcaaa cgggtaatgt cattcaaatg ctttttaatg
    17101 gacttactta aaccttgagc acctgtagca gagcgttccc tcatatccag ccaagcttta
    17161 gccttaaaaa taattaatac tcaatgaaaa tcaaagagca aactaggaag ctagctgcag
    17221 gctgctcaaa acactgtttt gaggttgtag atggaagctg acgtggtttg aagagatttt
    17281 cgaagagtat aaaccacaat tactcaatac cgaatacccc tgaatggttt ctttttcatg
    17341 caccaatata ttataataat cttcatccaa taataaggct gataaactag catcttggtc
    17401 aaaatgtaag ggaatttctc gaccgtcctt ctttaatgga tattctggta agatactaaa
    17461 tagttcaatc atagaaggaa actcaggatt agttgttgta aatcgaaaaa gctgcgcttt
    17521 ctcatctttc tgacttcctt gatactctcc caattctaaa aaatgattca aggtagtata
    17581 aaattcctta ttttttactt cataaatgat gaccatatca taatcttttg ttgtgcgact
    17641 tttaaatcct tgcgaatcca atacgataga ggtagcagtt cccccaatca gaacataata
    17701 gttctgaaaa tccgcaaaca tttcttgaaa aataactttc gtattagctg gcatcatctt
    17761 ctcccagata ctgtaatatc atattttcta gtgcttcact ctcttcctct atacgtgggt
    17821 catcatcatc ttttaaggtc aaataaagag aaatcggatc tacaaattgt ttatcatgat
    17881 tatttttaaa atcattccaa aactcagata caaaaggacg atatttccat atctctagca
    17941 tctttcattt taaaacatgc tgagaaagtg gcaaggataa ctgattgaat tttctctgcc
    18001 atatgacata gctagtattt tcatccgttt cagctaaaaa agttgaatgc gacaaagcat
    18061 aagcaccacc atatagaagg ttagaaacag attttatctg ctttatatcg ccatctggca
    18121 ataaaaaggc aatccacgtt aattgttcgc taggtgttaa ttccttaggg atttcagtat
    18181 cattcgcatt gagtactagt cccaatggag ggaagaagag gtttccctta aagtctacaa
    18241 acggaactct agcttgaagt aattgctttt tttcactgtc tgataacttc gaaaacacca
    18301 aaacaacatc catattggct ttttcaccca tagtgcgagc ctgagtaaca aatgaactca
    18361 aactccccct tctcttttct ttaatcaata aaaatgattc cttatgaaaa gtcatttgag
    18421 tatactcaaa cctttttaca aaactgattg gtaaagtcaa agtcagttcc tcaatactga
    18481 catcaacata aaaggattga aaaattgaca ttatcttttt gacattcatg ttttttcctt
    18541 attctatcat ttttattata ttttatcatt cggtataata aaatataagt tttttgataa
    18601 aataaccaaa aactactcgc tatcccctct cacattaccc tacatatcgt ttgacatgcg
    18661 actgatagtt caggaaaact tcaaggagaa cttttctctc atccactatg caggacttac
    18721 tatttcactt ctactcctat aggctcaatt tgacactttt ctttttcgaa ttgctcatat
    18781 gcctcctgaa tagtgagttc gatttatcca aatttatttt tgtctatttt gatgaatact
    18841 tccacgaaga tagtctgaaa atgaatttgc accaattttc cttctctttt taagcatatt
    18901 attccagata tgattgccct cttgttcagt aataaattac gctttaaacg cttcaatcag
    18961 tatatctcct gttgtcatat gttctaaaga aaattcttct acatatggtt taacatctct
    19021 taggttatta cttcctaata tcccattatg ctttttcgct aagaaaatag aagtcgcttc
    19081 tcccttacca ataatcttgt tactatcata atttcttgtt aaatctctat ataatgcgta
    19141 ttcttcagtt ccaatgtcta tgctcacaat ctcagctgaa cccttagtta ccaactggtc
    19201 tatcctagat tttaaatggg gaattgtagg tatattgatt tcatcataca cctcttgtgg
    19261 aataacaatt ttacccgaat agagcttttc taaaagatgt tcagtgccaa cccataaaaa
    19321 tgctgaaata caatctgcat caataaatac acgactagtc aaacgacaac tccccctctt
    19381 catctagccc atatacaata tcatatctga aagcatctag taacagttcc tcatacttcc
    19441 cttgcgaaat tctgttattt tctaaaagtt gttcagtcga tttaatatag tgtcctaata
    19501 ccatttcctt tttactttct gacaaaggac gatataaact tgtatcatag cctaatcttg
    19561 aagctgtctc tataacacta atatccatat ttttaatttc ttctgcatca aggtatccat
    19621 catttctcaa tctatataac atagctttat gactgatacc ataaaactga cccaatttta
    19681 taatatcttc tacttcaaga tgagttctat tggcattttc tctgatttcc tcaaccatcc
    19741 tatacagtga agatgggaaa attaaaaaat aagaagcaaa ctgatccgct tttctttcag
    19801 tttcatctcc ttcaccaatc aagataagac tgactgaact cttcttcacc tcatcataat
    19861 aaagatgata cagtccatgt gctaaagaaa atctttacct tcctaatggc atgtctgaat
    19921 tgactgcaat gagactgaac tgagttcctt tataacagac tccgctaata ttctttccga
    19981 gtccataaaa taccagcgtc aaattttcta tcttttgtac caatttaaaa atatctatcg
    20041 gcgattcacc atcagctccc aacttttttc taagatttga agctttatta cttaaatcca
    20101 ttcgtgatat tcctttcacc tttttccatt ttggcaatca tgctttgatt caaagacaaa
    20161 tactcagcaa tcttatcttg agtaagattt gaagatattc taagttcttt tattcttttt
    20221 ccaacatcac atacatttat catattcata cacctctcat aaa
//
LOCUS       DAVGJL010000020        19473 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.020, whole genome shotgun sequence.
ACCESSION   DAVGJL010000020 DAVGJL010000000
VERSION     DAVGJL010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19473
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.020"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            2..74
                     /locus_tag="VVE42_000659"
     tRNA            2..74
                     /locus_tag="VVE42_000659"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            99..171
                     /locus_tag="VVE42_000660"
     tRNA            99..171
                     /locus_tag="VVE42_000660"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:132..134,aa:Lys,seq:ttt)
     gene            177..258
                     /locus_tag="VVE42_000661"
     tRNA            177..258
                     /locus_tag="VVE42_000661"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:211..213,aa:Leu,seq:tag)
     gene            277..349
                     /locus_tag="VVE42_000662"
     tRNA            277..349
                     /locus_tag="VVE42_000662"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:310..312,aa:Thr,seq:tgt)
     gene            372..443
                     /locus_tag="VVE42_000663"
     tRNA            372..443
                     /locus_tag="VVE42_000663"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:404..406,aa:Gly,seq:gcc)
     gene            451..536
                     /locus_tag="VVE42_000664"
     tRNA            451..536
                     /locus_tag="VVE42_000664"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:485..487,aa:Leu,seq:taa)
     gene            546..619
                     /locus_tag="VVE42_000665"
     tRNA            546..619
                     /locus_tag="VVE42_000665"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:580..582,aa:Arg,seq:acg)
     gene            635..708
                     /locus_tag="VVE42_000666"
     tRNA            635..708
                     /locus_tag="VVE42_000666"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:669..671,aa:Pro,seq:tgg)
     gene            714..787
                     /locus_tag="VVE42_000667"
     tRNA            714..787
                     /locus_tag="VVE42_000667"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:748..750,aa:Met,seq:cat)
     gene            801..874
                     /locus_tag="VVE42_000668"
     tRNA            801..874
                     /locus_tag="VVE42_000668"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:835..837,aa:Ile,seq:cat)
     gene            885..974
                     /locus_tag="VVE42_000669"
     tRNA            885..974
                     /locus_tag="VVE42_000669"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:921..923,aa:Ser,seq:tga)
     gene            985..1058
                     /locus_tag="VVE42_000670"
     tRNA            985..1058
                     /locus_tag="VVE42_000670"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1019..1021,aa:Met,seq:cat)
     gene            1062..1134
                     /locus_tag="VVE42_000671"
     tRNA            1062..1134
                     /locus_tag="VVE42_000671"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1095..1097,aa:Phe,seq:gaa)
     gene            1154..1224
                     /locus_tag="VVE42_000672"
     tRNA            1154..1224
                     /locus_tag="VVE42_000672"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1186..1188,aa:Gly,seq:tcc)
     gene            1260..1333
                     /locus_tag="VVE42_000673"
     tRNA            1260..1333
                     /locus_tag="VVE42_000673"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1294..1296,aa:Ile,seq:gat)
     gene            1340..1427
                     /locus_tag="VVE42_000674"
     tRNA            1340..1427
                     /locus_tag="VVE42_000674"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1374..1376,aa:Ser,seq:gct)
     gene            complement(1553..2488)
                     /locus_tag="VVE42_000675"
     CDS             complement(1553..2488)
                     /locus_tag="VVE42_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HEU3072902.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTAIWKAIKRLEQE
                     GIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKE
                     KAASLFKATAPITRNELIIEIWRTFFETPAEELLYLYKKQSFILGKEVTFTLEQKDYK
                     GLAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK"
     gene            complement(2485..3345)
                     /locus_tag="VVE42_000676"
     CDS             complement(2485..3345)
                     /locus_tag="VVE42_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU3072903.1"
                     /translation="MLVFSEYQTGTIDLALSFYGYEECTPNYSFGPAIRDTYVLHYIT
                     KGQGKFYYKGKIVDLKEGDFFLLKPEELTFYQADSKEPWAYYWLGITGGKSPDYFALS
                     QISDQSYLIQSETCHTQTTAKLISDIVRFAQITKSSELAQLHIMGQLHELMFHLGTIA
                     PNQKKKNISSTHQLYLECKRLIDSHYPQSLTIQDLAKELSVHRSYLSSVFKEFNTLSP
                     KEYLLYVRMHRARQLLENTQESIKVIAYSVGFSDPLHFSKAYKQYFNQTPSHTRKEYS
                     QYQLVRKATL"
     gene            3453..5615
                     /locus_tag="VVE42_000677"
     CDS             3453..5615
                     /locus_tag="VVE42_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEU3072904.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENRNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHSVTEVTDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATIASYSKLD
                     NNSNQEVVIHKDFSFMADFPAADYEIVTLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHAQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRVAIGINPENFSW
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLKHIMLSEFSKKERPILINNWEATY
                     FDFQREKLLELADEAKKVGIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRRHPDWAIQVPDYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQLLSYHDIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMMRYFPQVWASDNTDAIARLPIQYGSSYLYPTISMGA
                     HVSAVPNHQMGRMTPLETRGLVAMMGNLGYELDLTNLSDEEKATIANQVNLYKELRPV
                     VQLGQQYRLINPDTVSNEAAVQFNYGNQTIVTYVRVLSVVETMETTLKLKNLDEEGLY
                     KLQENGEVYSGAELMYAGLTVILSQGDFLSRQYIFRRL"
     gene            5650..6909
                     /locus_tag="VVE42_000678"
     CDS             5650..6909
                     /locus_tag="VVE42_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU3072905.1"
                     /translation="MEWYKKIGLLATTGLALVGLGACSNYGKSADGTVTIEYFNQKKE
                     MTKTLEEITRDFEKENPKIKVKVVNVPNAGEVLKTRVLAGDVPDVVNIYPQSIELQEW
                     AKAGVFEDLSNKDYLKRVKNGYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKV
                     PETWDEFEQLVKDIVAKGQTPFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQ
                     PNAIKLSDPIMKDDIKVMDILRINGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAI
                     TAINEQKPNFKIGTFMIPGKEKGQSLTVGAGDLAWSISATTKHPKEANAFVEYMTRPE
                     VMQKYYDVDGSPTAIEGVKQAGEDSPLAGMTEYAFTDRHLVWLQQYWTSEADFHTLTM
                     NYVLTGDKQGMVNDLNAFFNPMKADVD"
     gene            6923..7789
                     /locus_tag="VVE42_000679"
     CDS             6923..7789
                     /locus_tag="VVE42_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU3072906.1"
                     /translation="MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLT
                     YNYKFVGLNNFKLLFMDPKFMNAIGFTAIITIAMVVGEIALGIFIARVLNSKIKGQTF
                     FRAWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFLQTSLLGTKWGAIFAAVFV
                     LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSIVFILA
                     LKGGLTAFDQVFAMTGGGPNNATTSLGLLVYNYAFKNNQFGYANAIAVILFFLIVVIS
                     IIQLRVSKKFEI"
     gene            7803..8636
                     /locus_tag="VVE42_000680"
     CDS             7803..8636
                     /locus_tag="VVE42_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU3072907.1"
                     /translation="MKQDERKALIGKYILLILGSVLILVPLLATLFSSFKPTKDIVDN
                     FFGFPTNFTWDNFSRLLADGIGGYYWNSVVITVLSLLAVMIFIPMAAYSIARNMSKRK
                     AFTIMYTLLILGIFVPFQVIMIPITVMMSKLGLANTFGLILLYLTYAIPQTLFLYVGY
                     IKISIPESLDEAAEIDGANQFTTYFRIIFPMMKPMHATTMIINALWFWNDFMLPLLVL
                     NRDSKMWTLPLFQYNYAGQYFNDYGPSFASYVVGIISITIVYLFFQRHIISGMSNGAV
                     K"
     gene            8637..8717
                     /locus_tag="VVE42_000681"
                     /pseudo
     CDS             8637..8717
                     /locus_tag="VVE42_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            8994..10436
                     /gene="gtfA"
                     /locus_tag="VVE42_000682"
     CDS             8994..10436
                     /gene="gtfA"
                     /locus_tag="VVE42_000682"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEU3072908.1"
                     /translation="MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSAFGDWEDVKRLGERYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKDLFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTELLPEIHEHYSIQFKIADHDYYVYDFALPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYASNELYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGR
                     NINRHYYSNEEIAKEVQRPVVKALLNLFSFRNRSEAFDLEGTTEIETPTAHSIVIKRQ
                     NKDKSVTAVAEIDLQNQTYRVIENGVEVTF"
     gene            10574..11767
                     /locus_tag="VVE42_000683"
     CDS             10574..11767
                     /locus_tag="VVE42_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEU3072909.1"
                     /translation="MKSIGFIEKLKGLSSKELILLGIILSIFLPFYLFVVVLCLYIIS
                     LIFTGDMKSILQKMGEHPMLLLFLSYSTVISILAQNWMGLVASVGMFLFTIFFLHYQS
                     ILSHKFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFF
                     NPNYYGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAG
                     AIIYLFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMAL
                     FKQNPFWGEGPLTYMHSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRL
                     MMDMSQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLV
                     SDMTD"
     gene            12454..14433
                     /locus_tag="VVE42_000684"
     CDS             12454..14433
                     /locus_tag="VVE42_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU3072910.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAAKETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KVVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
     gene            14500..15996
                     /locus_tag="VVE42_000685"
     CDS             14500..15996
                     /locus_tag="VVE42_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU3072911.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDSAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGTLTFLLALPTIALVY
                     IVRLIGSSIALPDSFPILGAGDWRSYVLPAVILGLLGAPVTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPGAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            15996..16922
                     /locus_tag="VVE42_000686"
     CDS             15996..16922
                     /locus_tag="VVE42_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU3072912.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSVRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            16931..17998
                     /locus_tag="VVE42_000687"
     CDS             16931..17998
                     /locus_tag="VVE42_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3072913.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIAQGSIDYRGQDLTALSSHKDWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYDPRHPYTWSLLSSLPQLADDKGDLYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSETHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            18009..18935
                     /locus_tag="VVE42_000688"
     CDS             18009..18935
                     /locus_tag="VVE42_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3072914.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQNIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQAELARYQKGLN"
BASE COUNT         5639 a         3517 c         4191 g         6126 t
ORIGIN      
        1 aggtcccgta gtgtagcggt tatcacgtcg ccctgtcacg gcgaagatcg cgggttcgat
       61 tcccgtcggg accgtttaag ataacgcaag ttattttaga ctcgttagct cagttggtag
      121 agcaattgac ttttaatcaa tgggtcactg gttcgagccc agtacgggtc atatatgcgg
      181 gtttggcgga attggcagac gcaccagatt taggatctgg cgcttaacgg cgtgggggtt
      241 caagtccctt aacccgcatt aagatataac aaatgagccg gcttagctca gttggtagag
      301 catctgattt gtaatcagag ggtcgcgtgt tcaagtcatg tagccggcat ttttagatag
      361 aagaaaacag tgcgaacgta gttcagtggt agaacaccac cttgccaagg tgggggtcgc
      421 gggttcgaat cccgtcgttc gcttagagag gccggggtgg cggaactggc agacgcacag
      481 gacttaaaat cctgcgattg gtaacgatcg taccggttcg attccggtcc tcggcataat
      541 atggagcacc cttagctcaa ctggatagag tacctgacta cgaatcaggc ggttagaggt
      601 tcgactcctc tagggtgcat ttttctattt aactcgggaa gtagctcagc ttggtagagt
      661 acttggtttg ggaccaaggt gtcgcaggtt cgaatcctgt cttcccgatt catggcggtg
      721 tagctcagct ggctagagcg tccggttcat acccgggagg tcgggggttc gatccccttc
      781 gccgctataa tgatcttgtc ggacctttag ctcagctggt tagagctctc ggctcataac
      841 cgagtggtcg taggttcaag tcctacaagg tccatttgaa tattggagga ttacccaagt
      901 ccggctgaag ggaacggtct tgaaaaccgt caggcgtgta aaagcgtgcg tgggttcgaa
      961 tcccacatcc tccttttata ttaacgcggg atggagcagc tcggtagctc gtcgggctca
     1021 taacccgaag gtcgtaggtt caaatcctgc tcccgcaata aggctcggta gctcagttgg
     1081 tagagcaatg gattgaagct ccatgtgtcg gcggttcgat tccgtctcgc gccattttat
     1141 tgatagtatt tatgcgggtg tagtttagtg gtaaaactac agccttccaa gctgttgtcg
     1201 cgagttcgat tctcgtcacc cgctttgaac tttgttcaaa ttaccaagtt ttttaacttg
     1261 ggcgcgtagc tcaggtggtt agagcgcacg cctgataagc gtgaggtcgg tggttcgagt
     1321 ccactcgtgc ccattaattg gagaattact caagaggctg aagaggacgg tttgctaaat
     1381 cgttaggtcg ggtaactggc gcaagggttc gaatccctta ttctccgttt taatgagagt
     1441 ttgtaactct cttttttatt tttagagaca ataaaacttc agatccgtaa aacttaattg
     1501 aattactaat ctgaagttat ttttatatcg tttatattat aattgtgtta ttttacttcc
     1561 aactattgag agaaatttcg ccactattta gccagatttc ttttccgtta tcacattgaa
     1621 ctaaaagttt tccattttct gagatgtctt tagcaagtcc cttgtagtct ttttgctcta
     1681 gtgtgaaagt gacttctttt cctagaatga atgactgttt tttgtatagg tataatagct
     1741 cttctgctgg tgtttcgaag aaagtacgcc agatttctat gatcaattca ttccttgtta
     1801 taggagctgt agctttaaat aagctggcag ctttttcttt taattcctga gggaagtctt
     1861 taatagtgaa attgatacct actccaataa tgatatctgt gactaagcca gtttctacag
     1921 aggtcattgc ttcagtaagg attcctccaa ttttatgatt gtttagatag atatcattga
     1981 cccattttat gtcgacatct attaaagtta ggttcttaat ggctttgtag acagctccag
     2041 ctacaagtag tgtgtaggat ggtaatttgt cataggggag atttggttta agatggagtg
     2101 tcatataaat accaccttgt ggtgagtaga aggaacgttg aaaacggcct cggcctgctg
     2161 tttgatagga agctagatag agggtatttg cttcatggcc taaatcaatt gcttcttttg
     2221 catctagttg tgttgatttt gtttcgggtt taaagctgac tttaattgga agattttctt
     2281 ctagaatctc tggaagaata aggtcaccat tcatcagttt atatcctcta tttttgatac
     2341 tatcaatttc aatgccttct tgttctagtc gcttgatggc tttccaaatt gctgttcggc
     2401 ttagggatag tttttctgcg attttttctc cgctgatata gtcggtttct ttagatagga
     2461 tttggtagac agcttggtag gatttcataa tgttgccttt ctcactagtt ggtattgaga
     2521 gtattctttt cttgtatgac ttggagtctg attaaagtat tgtttataag ctttcgaaaa
     2581 atggagtgga tctgaaaaac ctaccgagta tgcaattacc ttgatggact cttgggtatt
     2641 ttcgagaagt tgtctagctc ggtgcattcg aacgtagagt aggtattctt tgggtgataa
     2701 ggtattaaat tctttgaata cgcttgataa gtagcttctg tgaacggata gttcttttgc
     2761 taaatcttga attgtaagtg attgaggata gtggctatca attaatcgtt tgcattcaag
     2821 atagagttgg tgggttgatg aaatattctt ttttttctga ttgggagcaa tagttcccag
     2881 atgaaacatc agttcatgaa gttgtcccat gatatggagt tgagctaatt cacttgattt
     2941 tgtaatctga gcgaagcgga caatgtctga gatgagtttt gcagtagtct gggtatgaca
     3001 agtttcagat tggatgagat aggattgatc agaaatttga gaaagagcaa aataatcagg
     3061 ggatttccct ccagtgattc ctaaccagta gtaggcccaa ggttctttac tatctgcttg
     3121 ataaaaggtt agttcctctg gttttaatag aaagaaatct ccttctttta aatcaacaat
     3181 tttacccttg taataaaatt ttccttgtcc tttagtaatg taatgtagaa cgtatgtatc
     3241 acgaatggct ggaccaaaag agtaattagg tgtgcattcc tcatatccat aaaagcttag
     3301 ggcaaggtcg attgttccag tctggtattc tgaaaaaact aacatgtgct acctcctacc
     3361 taacatttta ccatattctt gagacatttt tctattttgg aaagcgattt caggtgataa
     3421 aatataatca ataaagtgat gaggtgaagt aaatgggagt taggatagag aataatctat
     3481 tttatgttga gagtaaaaat ctaagtttga ttattgaaaa tcgaaatggc tacttacttt
     3541 tgaaacattt aggaaagact attaagaact ataaaggttc caatagtgtt tatgaacgag
     3601 accatgcctt ttcaggaaat ccaacggcta ctaatcgaac ctttagttta gatactcagc
     3661 gacagatttt tggacaacat ggcttaggag attttaggaa accaaccata caggttcagc
     3721 atagtgtaac tgaagtaaca gactttcgat ttgtagaagc aaagatttta aaaggtcaga
     3781 atggtccaca gggcttacct tctccacata gcatggacga tacagagact cttgtcttaa
     3841 tgttagaaga ttctaaggct caacttagtc tgactttgta ttatactact tttaataatg
     3901 atgcgactat tgctagctac agtaaattag ataataatag taatcaggaa gttgtcatcc
     3961 ataaagattt ttcttttatg gctgattttc cagctgcaga ttacgaaata gtaactctgc
     4021 agggtgctta tgctcgtgaa aagactgtta gacgtcaaca ggtagaacaa ggaatctttt
     4081 cgattagctc aaaccgaggt gcttctggtc atgctcaaac accagctctt ctactatgcg
     4141 aacaaggagt cacagaggat gctgggaatg tgtttgctat tcaactaatg tatagtggca
     4201 actttgaagc ttttgttcaa aaaaatcaat tgaatgaagt tcgggtggct attggcatta
     4261 atccagaaaa cttttcttgg aagttagctc ctgaggaata ctttgaaaca ccggtagctt
     4321 tagtgaccca ttcagatcag ggattaactg gtattagtca tgaaagtcag aattttgtac
     4381 tgaagcacat tatgctaagt gaattttcta aaaaagaacg tccaattcta atcaataact
     4441 gggaagctac ttactttgac tttcagagag aaaaactgtt agagttagca gatgaagcta
     4501 agaaagttgg cattgaactt tttgtattag atgatggttg gtttggcaat cgttttgatg
     4561 ataatcgtgc tttaggtgat tgggttgtta atgaggaaaa actgggtgga agtctagaaa
     4621 gtctgatttc agctatccat gaaagaggtt tgcagtttgg actttggtta gaacccgaaa
     4681 tgatttctgt agatagtgat ttgtatcgtc gacatcctga ctgggctatt caggttcctg
     4741 actatgagca tacttattct cggaatcaat tagtacttaa tcttgccaat cctcaggtag
     4801 tagaatactt gaaaagtgtc ttagatcaac tcctatctta tcatgatatt gattacatta
     4861 agtgggatat gaaccgcaat atcactaagc tagggaatgg attaacttat ctagagacac
     4921 agatgcaatc tcatcagtac atgctggggc tttacgaact cgtttcttat ctgacagaga
     4981 agcacagcca tattctcttt gagtcctgct ctggtggtgg tggacgaaat gatcttggta
     5041 tgatgcgcta tttcccacaa gtctgggcta gtgataatac tgatgccatt gcacgtttac
     5101 caattcaata cggttcatcc tatctctatc caaccatttc tatgggggct catgtgtcag
     5161 cagtaccgaa tcatcagatg ggacgaatga caccattaga aacacgtggc cttgtagcaa
     5221 tgatgggaaa tttgggctat gaacttgatt tgacaaattt atcagatgaa gagaaagcta
     5281 cgattgctaa tcaggtgaac ttgtataaag aattacgacc agtagttcag ttaggacagc
     5341 agtatagact aattaatcct gatactgtat ccaatgaagc agctgtacaa tttaattacg
     5401 gaaatcaaac gattgtaacc tacgttcgcg ttttatctgt tgtagagacc atggaaacaa
     5461 ctttaaaatt aaaaaatttg gatgaagagg gactatataa attacaggaa aatggcgaag
     5521 tttactcagg tgcagaacta atgtatgcgg gcctaactgt tatcttatcc caaggagatt
     5581 ttttgagtag acagtatatt tttagaagac tatgataagg gtgtataaat ttataaagga
     5641 ggctttctaa tggaatggta taaaaaaatc ggacttcttg caactacagg tttagctttg
     5701 gttgggctcg gcgcttgctc caactatggt aaatctgcgg atggcacagt gaccatcgag
     5761 tatttcaacc agaaaaaaga aatgaccaaa accttggaag aaatcactcg tgattttgag
     5821 aaggaaaacc ctaagatcaa ggtcaaagtc gtcaatgtac caaatgctgg tgaagtattg
     5881 aagacacgcg ttctcgcagg agatgtgcct gatgtggtca atatttaccc acagtccatc
     5941 gaactgcaag aatgggcaaa agcaggtgtc tttgaagatt tgagcaacaa agactacctg
     6001 aaacgcgtga aaaatggcta cgctgaaaaa tatgctgtaa acgaaaaagt ttacaacgtt
     6061 ccttttacag ctaatgctta tggaatttac tacaacaaag ataaattcga agaactgggc
     6121 ttgaaggttc ctgaaacctg ggatgaattt gaacagttag tcaaagatat cgttgctaaa
     6181 ggacaaacac catttggaat tgcaggtgca gatgcttgga cactcaatgg ttacaatcaa
     6241 ttagcctttg cgacagcaac aggtggagga aaagaagcaa atcaatacct tcgttattct
     6301 caaccaaatg ccattaaatt gtcggatccg attatgaaag atgatatcaa ggtcatggac
     6361 atccttcgca tcaatggctc taagcaaaag aactgggaag gtgctggcta taccgatgtt
     6421 atcggagcct tcgcacgtgg ggatgtcctc atgacaccaa atgggtcttg ggcgatcaca
     6481 gcgattaatg aacaaaaacc gaactttaag attgggacct tcatgattcc aggaaaagaa
     6541 aaaggacaaa gcttaaccgt tggtgcggga gacttggcat ggtctatctc agccaccacc
     6601 aaacatccaa aagaagccaa tgcctttgtg gaatatatga cccgtccaga agtcatgcaa
     6661 aaatactacg atgtggacgg atctccaaca gcgatcgaag gggtcaaaca agcaggagaa
     6721 gattcaccgc ttgctggtat gaccgaatat gcctttacgg atcgtcactt ggtctggttg
     6781 caacaatatt ggaccagtga agcagacttc cataccttga ccatgaacta tgtcttgacc
     6841 ggtgataaac aaggcatggt caatgatttg aatgccttct ttaacccgat gaaagcggat
     6901 gtggattagg agtagagaga ctatgaaaaa agtattacaa aaatattggg catgggcttt
     6961 tgtggtcatc cccctcttgt tacaagcaat tttcttctat gtgccgatgt ttcaaggagc
     7021 cttttacagt tttaccaact ggacaggatt gacttataac tacaaatttg tcggcttaaa
     7081 caactttaag ctcctcttca tggatccaaa attcatgaat gcgattggct ttaccgcaat
     7141 cattacgatt gccatggtgg ttggtgagat tgcactcggg atcttcattg cgcgtgtctt
     7201 gaactctaaa atcaaaggcc aaaccttctt ccgtgcttgg ttcttcttcc cagctgtttt
     7261 atctggtttg acagtggctt tgatcttcaa gcaagtcttc aactacggtc ttccagcgat
     7321 tgggaatgcc cttcatattg aatttctcca aaccagtctt ttagggacta agtggggagc
     7381 aatctttgcg gctgtctttg tccttctttg gcaaggggtg gctatgccca tcatcatctt
     7441 cctagctggt ttgcaatcta ttccaactga gattacagag gcagcaagga ttgatggtgc
     7501 gactagcaag caagttttct ggaacattga attgccttac ttgctaccaa gtgtctctat
     7561 agtctttatc ctagccctaa aaggtgggct gactgccttt gaccaagtct ttgccatgac
     7621 cggtggtggt ccaaacaatg ccacaacctc acttgggctc ttggtttata actatgcctt
     7681 taaaaacaac caattcggtt atgccaatgc cattgccgta atcttgttct tcttaattgt
     7741 agtgatttcg atcatccaat tgagagtatc taagaaattt gaaatttaag aggagaagca
     7801 tgatgaaaca agatgaaaga aaagccctga ttggcaaata cattctattg attctaggat
     7861 cggttctgat tttagtgccg ctccttgcta ccctctttag ttctttcaaa cccactaagg
     7921 atattgtaga taatttcttt ggctttccaa ccaacttcac atgggacaac tttagccgtc
     7981 tcttagctga tgggattgga ggctattatt ggaactctgt cgtcatcact gtcttgtctt
     8041 tacttgcagt aatgatcttt atccctatgg cagcctactc catcgctcgc aatatgagta
     8101 aaagaaaagc ctttaccatc atgtataccc tcttaatcct cggaatcttc gtccctttcc
     8161 aagtcatcat gattccgatt acggttatga tgagtaaact cggtttggct aatacctttg
     8221 gtttgatctt gctctacttg acctatgcga ttccacagac cctctttctc tatgttggct
     8281 atatcaaaat ctcgattcca gaaagtctgg atgaagcagc agagatcgat ggggctaatc
     8341 aatttacaac ctatttccgc atcatcttcc caatgatgaa accgatgcat gcgacaacca
     8401 tgatcatcaa tgccctttgg ttctggaatg acttcatgtt gccactcctt gtcttgaacc
     8461 gggattccaa aatgtggact ctgcctttgt tccaatacaa ctacgcaggc caatatttca
     8521 acgactacgg accaagcttt gcctcttacg tggtcggcat tatcagtatc accattgtct
     8581 atctcttctt ccaacgccat atcatttcag gaatgagcaa cggggcagtg aagtaatact
     8641 caatgaaaat caaagagcaa actaggaagc tagccgcagg ttgctcaaaa cactgttttg
     8701 aggttgtgga tgaaactgac gaagtcagta accataccta cggtaaggca acgctgacgt
     8761 ggtttgaaga gattttcgaa tagtataaga agagctcatg gaatctatag tctttgacac
     8821 agaggaagag ttcagcttgt tgataattct atgtagaaaa caagaaagta gtactttgtt
     8881 tatcacagtc tatattagta cgagtttgat gataaataga gatttaaaat gaattttatt
     8941 tatgaagcca gatcatatga gacttggctt caattagaaa aaggagagta atgatgccaa
     9001 ttcaaaataa aaccatgttg attacctatt ctgatagcct tggaaataat cttaaagact
     9061 tatatgataa tttggaagag cattttggag atgctattgg aggagttcac cttttaccat
     9121 ttttcccatc aacaggtgat cgtggatttg cgccagttga ctacgacgaa gtggactcag
     9181 cttttggtga ttgggaggat gttaagcgtt taggtgagag atattatctt atgtttgatt
     9241 ttatgattaa tcatatttct cgtcaatcca agtattataa ggactatcaa gaaaaacatg
     9301 aagccagtga atttaaagat ctctttttaa actgggataa gttttggcca gaaaaccgtc
     9361 cgacacagtc tgatgtagat ttaatttaca agcgtaagga tcgtgcacca aagcaagaga
     9421 ttgtgtttga agatggttca gtggaacatt tgtggaatac ctttggtgag gagcagattg
     9481 atcttgatgt gaccaaagaa gtaactatgg aatttatccg taagaccatt cagcacttgg
     9541 caagtaatgg gtgtgatttg attcgtctag atgcctttgc ttatgcagtg aagaaattgg
     9601 atactaatga tttctttgtg gaaccagata tttgggattt attggacaaa gttcgagata
     9661 tcgctgctga gtatgggaca gagcttttac ctgagattca tgaacactat tcgattcagt
     9721 ttaaaatagc agaccatgat tactatgttt atgattttgc tcttccaatg gtgacacttt
     9781 atactcttta cagttccaga acagagcgtt tggctaagtg gttaaagatg agcccgatga
     9841 agcaatttac gacgctagat acccatgatg ggattggagt agtagatgtc aaggatatcc
     9901 tgaccgatga ggagattgac tatgcttcaa atgaacttta taaggttgga gccaatgtca
     9961 aacgtaagta ctctagtgcc gagtataaca acttagatat ctaccaaatc aattcaacct
    10021 actattcagc gcttggagat gatgatgtca agtattttct cgctcgtcta attcaagctt
    10081 ttgccccagg tattcctcag gtttactatg tgggtctatt agcaggcaag aatgacttga
    10141 aattattaga agaaactaaa gaaggtcgaa atattaatcg tcattactat agcaacgagg
    10201 aaatagcaaa agaagtgcaa cgacctgttg tgaaggccct tctcaatcta ttttctttcc
    10261 gtaaccgttc agaagccttt gatctagaag ggactactga gatagagaca ccaacagctc
    10321 acagcattgt aatcaaacgt caaaataaag ataagtccgt aacagcagta gcagaaattg
    10381 atttgcaaaa tcagacttat cgggtaattg agaatggagt tgaagtaaca ttttgaagcc
    10441 ttgatatggt ggataaaata gggtttttat tttggaaaac gtttcctttg ttttcaaatt
    10501 gctaaaaaag tggtacaata taggatagct tactattatc tgaatcagct gatttggaga
    10561 gaaaggattc attttgaaat caataggctt tattgaaaag ctgaaggggt tgtctagtaa
    10621 agagctgatt ttattgggaa ttatcctgag tatcttttta cccttttatc tttttgtagt
    10681 tgtactctgt ttatatatta tcagtttgat ttttacagga gacatgaaaa gtattcttca
    10741 gaaaatgggg gagcatccga tgctgcttct ttttcttagc tatagtactg ttatatccat
    10801 tcttgcacaa aattggatgg gtcttgtggc ttcagtagga atgtttctat ttactatttt
    10861 ctttttgcac tatcagtcga ttttatccca taaattcttt cgattgattt tgcagttcgt
    10921 cttgtttggt agtgtcttgt cagctgcttt tgccagttta gaacatttcc aaattgtgaa
    10981 gaaatttaac tatgcttttc tttcacccaa tatgcaggtg tggcatcaga accgggcaga
    11041 agtgaccttc tttaatccta attattatgg aattatttgt tgtttctgta ttatgattgc
    11101 tttctatctg tttacaacga ccaagttgaa ttggttgaaa gtattctgtg tgattgcagg
    11161 ctttgttaat ctctttggtt tgaactttac tcaaaatcga actgcctttc ctgctattat
    11221 cgctggagca attatctatc tctttacgac tattaaaaac tggaaggcct tttggcttag
    11281 tattggggtc ttcgcgattg gtttgagttt cctcttttct agtgatttgg gagttcgaat
    11341 gggtacttta gactcttcta tggaagaacg catttctatc tgggatgctg ggatggcctt
    11401 gtttaagcaa aatccttttt ggggtgaagg gccattgacc tatatgcact cttatcctcg
    11461 gatacatgct ccttatcatg aacatgccca cagtctttat attgatacga ttctgagtta
    11521 cggaattgtg ggtaccattt tattagtttt gtcttctgtt gctcctgttc gcttgatgat
    11581 ggatatgagt caggagtcgg ggaaacgtcc gattatcggc ctttatctat ctttccttac
    11641 agtggttgct gtgcacggaa tttttgactt ggctctcttc tggattcagt caggctttat
    11701 tttcttgcta gttatgtgca gtattccatt ggagcatcga atgttggtat cggacatgac
    11761 ggattaagtt ttataagttt gaaatcttct accttgggta ggagattttt ttatacgatg
    11821 ggcatgtcgt gcatctgagg tgtaagtcct ctgtagggat ccactaccgg tgaatacaat
    11881 agcgctcccc aagcctgact atcgtgaggt agcggattaa aatggtctgg ggatagatcg
    11941 ttttaagtct gacgcaggaa ataagaattg tcagagaaag caatagtgaa atcgtaggct
    12001 atgaatagaa acgtgatagc gaggagcata tagtggataa ggtagcgaga aattttaaag
    12061 tataaaagat agtcttaatc tatatatgta aatcacaaaa gtaattgaat tcggaataag
    12121 gttggtgtga aaagacatag taaactgaat cttgaatact agaatgatac ttctacaata
    12181 ttgttataat ttaatttgaa ttccaaaatc acattgttcc gtccgtattt cattttactg
    12241 tatgtcttcc taaactccaa agtttctgcg tcaaatttcc tattttcact gtagtctttg
    12301 acacttcgaa cgacaatttg aatcatatat tcaaaaaaaa atgaaaaatt tgtgccaaca
    12361 agcctaaata ttctgaaaat ttgtttgatt tttaatggat aatgtgatat aatggttgtc
    12421 aatctataat tcacataaac agaggagtag aaaatgaaaa aaaatcgtgt atttgctaca
    12481 gcaggtcttg ttttattagc agcaggtgta cttgcagcat gcagttcttc aaaatcatct
    12541 gattcatcag cccctaaagc ttatggctat gtttatacag cagacccaga aaccttggac
    12601 tacctgattt caagtaaaaa tagtacaaca gtagtgactt caaatgggat tgatggttta
    12661 ttcactaacg ataattacgg taatcttgct cctgcagttg cagaggattg ggaagtctct
    12721 aaggatggtt tgacctacac ttataagatt cgtaaagggg ttaaatggtt tacctctgat
    12781 ggagaagaat atgcagaggt gacggctaaa gatttcgtga acggtttaaa acacgcagca
    12841 gataaaaaat cagaagctat gtatttagct gaaaattcgg ttaaaggctt ggcagattat
    12901 ctatcaggaa cttcaacaga tttttcaaca gttggtgtca aggcggttga tgattatacg
    12961 ttacaataca ctttgaacca gcctgaaccg ttctggaact ctaagttgac ctattctatt
    13021 ttctggcctc tgaatgaaga attcgaaaca tcaaaaggaa gcgattttgc taaaccaaca
    13081 gatccgacat ccttgcttta taatggtcca ttcttgttga aagggttgac tgcaaaatct
    13141 tctgtagagt ttgtaaaaaa tgagcaatat tgggataaag aaaatgtcca cctagatact
    13201 atcaatctag cttactatga tggatcagat caggagtcgc tagagcgtaa cttcactagt
    13261 ggagcttata gttatgcccg tctttaccct accagctcca actattctaa ggttgcagaa
    13321 gaatacaagg acaatatcta ttacacacaa tcaggctctg ggattgctgg tctgggtgtg
    13381 aatattgatc gccaaagtta caactatact tctaaaacta cagattcaga gaaagtagct
    13441 actaagaagg cattgcttaa caaagatttc cgtcaagcct tgaattttgc tcttgatcgc
    13501 tcagcttact cagctcaaat caatggtaaa gatggagcag ctttagcagt tcgtaattta
    13561 tttgtaaaac cagactttgt ttcagctggt gagaagacct ttggtgattt agtcgctgct
    13621 caacttcctg cttatggtga tgagtggaaa ggtgtgaatt tagctgatgg gcaggatggt
    13681 ttattcaatg ctgacaaggc caaggcagag tttgcgaaag ctaagaaagc tttagaagca
    13741 gacggcgttc agtttcctat tcatctggac gttccagtag accaagcatc aaaaaactac
    13801 atatctcgta ttcagtcctt taaacaatct gtagaaacag ttcttggtgt tgaaaatgtc
    13861 gttgttgata ttcaacaaat gacaagtgat gaattcctta atattactta ctatgctgcc
    13921 aatgcttcat ctgaggattg ggatgtatcg ggaggagttt catgggggcc agactatcaa
    13981 gacccatcta cttacctgga tattttaaaa acaactagca gtgaaactac aaaaacatat
    14041 ttaggatttg ataatccaaa tagcccttca gtagttcaag ttggtttgaa agaatacgat
    14101 aaattagttg atgaagctgc caaagagaca agcgacttga atgtccgtta tgaaaaatat
    14161 gcagcggctc aagcatggtt gacagatagt tcactcttta ttcctgctat ggcttcttct
    14221 ggtgcagcac cagtgctttc acgaattgtt ccatttactg gagcttctgc gcaaacaggc
    14281 tctaaggggt cagatgttta cttcaaatat ttgaaattac aagataaagt ggtgactaag
    14341 gaagagtatg aaaaagctcg tgaaaaatgg ttgaaagaaa aagctgaatc aaatgagaaa
    14401 gctcaaaaag aattggcaag tcatgtgaag taaataattt ctaatagaac tttctctcca
    14461 tgaggagaag gttcttttgg gatttttaaa ggaaataata tgaaaaaata tatttttatg
    14521 cgtgttttgc ggtcattggt ttcgattttc ttagtaacga ctttgaccta cacgattatc
    14581 tataccttgg ttcctcgaaa attgattttc aagcaggatc ctaactataa taaaattgcg
    14641 acaacggctg ataaacgtga taactatgaa aatactgtgt ttgagcgtat gggctacatt
    14701 gagtattacg atactaaaga gttgcaagaa aaggcaagta gcatggattc ttctgtaaca
    14761 gtagaagcaa atgcgaccaa taaagctatt tatgaaaagt acatcaatca attaggtcat
    14821 ggttggactt tgggagaatt tactgaaagt ggtcaattct atgctactcg tgaaattcca
    14881 atttttgaac gtgtttttca cttctatgct aacttgattg acattgacca tacaaataaa
    14941 atccaagacc ctgaaaatcc agacttgaaa cgctaccttc gttttgaaaa tgattcagct
    15001 atcggatggt cattggtcgg ttcaggaact aaacataaat atctcttgta ctttaacagt
    15061 cagttcccat ttgttcatca aaactttgtg aacttgaatt taggtgactc ttacccaacc
    15121 tatgctaata caccagttct tcaggttatt actcaaggtc aaggacaaac caaaactgcc
    15181 caagttcagt tcccaacagg taagaaaacg tcttctgtaa atatttactc aagaacctac
    15241 aagtcaccta gtcaggctga ctctcgtgaa gtagctagct atgggaaaga tgatccttat
    15301 acagcgactg aaagtaatta ccaatatcca tctatgattg tcagctctgc tattactggt
    15361 ttgattggtt tggttcttgc ctatgctctt gccgtgccac ttggttcagc catggctcgt
    15421 ttcaagaaca cttggattga tagcctctca acagggactt tgaccttctt gcttgctctt
    15481 ccaacgattg ccttggttta catcgttcga ttgattggat catctattgc ccttccagat
    15541 tcattcccta tcttgggagc tggagattgg cgttcttacg ttttaccagc agtcatcctt
    15601 ggtttgttgg gtgctcctgt tacagccatt tggattcgtc gttacatgat tgacttgcaa
    15661 tctcaagact ttgttcgttt cgctcgtgca aaaggtttgt ctgaaaaaga aatttcaaac
    15721 aaacacatct ttaaaaatgc catggttccg ctggtttcag gaattcctgg tgccattatt
    15781 ggggttatcg gtggtgcaac ccttactgaa acagtcttcg ccttcccagg tatgggtaaa
    15841 atgttgattg actctgtaaa agcatctaat aactctatgg tcgttggtct tgtcttcatc
    15901 tttacatgta tttctatctt ctcacgtctt ttgggagata tttggatgac tattattgac
    15961 ccacgtatta aattgactga gaaaggaggc aaataatgtc tacaatcgat aaagaaaaat
    16021 ttcagtttgt aaaacgtgac gattttgcct ctgaaactat tgatgcgcca gcatattctt
    16081 actggaaatc agtgtttaaa caatttatga agaaaaaatc aactgtagtc atgttgggaa
    16141 tcttggtagc catcattttg ataagtttca tctacccaat gttttctaag tttgatttca
    16201 atgatgtcag caaggtaaac gactttagtg ttcgttatat caagccaaat gcggagcatt
    16261 ggttcggtac tgacagtaac ggtaaatcgc tctttgacgg tgtctggttc ggagctcgta
    16321 actccatcct catttctgtg attgcgacag tgattaactt ggttatcggt gtttttgtcg
    16381 gtggtatttg gggtatttca aaatcagttg accgtgtcat gatggaagtt tacaacgtca
    16441 tctcaaacat cccacctctt ttgattgtta ttgtcttgac ttactcaatc ggagctggat
    16501 tctggaatct gatttttgcc atgagcgtaa caacatggat tggtattgcc ttcatgatcc
    16561 gtgtgcaaat cttgcgctat cgtgacttgg aatacaactt ggcgtcacgt actttgggaa
    16621 caccaacctt gaagattgtt gccaaaaata tcatgcctca attggtatct gttattgtga
    16681 caaccatgac tcaaatgctt ccaagcttta tctcatacga agccttcttg tctttcttcg
    16741 gtcttggatt accgattaca gtgccaagtt tgggtcgttt gatttcggat tattcacaaa
    16801 acgtaacaac caatgcttac ttgttctgga ttccattgac aacccttgtc ttggtatcct
    16861 tgtccctttt cgtagttggt caaaacttag cggatgctag tgatccacgt acacatagat
    16921 aggagtagaa atgacaaaag aaaaaaatgt aattttgact gctcgcgata ttgtcgtgga
    16981 atttgacgtt cgtgacaaag tattgacagc cattcgcggc gtttcccttg aactagtcga
    17041 aggagaagta ttagccttgg taggtgagtc aggatcaggt aaatctgttt tgacaaagac
    17101 cttcacaggt atgctcgaag aaaatggtcg tattgcccaa ggtagtattg actaccgtgg
    17161 tcaggacttg acagctttat cttctcacaa ggattgggaa caaattcgtg gtgctaagat
    17221 tgcgactatc ttccaggacc caatgactag tttggacccc attaaaacaa ttggtagtca
    17281 gattacagaa gttattgtaa aacaccaagg aaaaacagct aaagaagcga aagaattggc
    17341 cattgactac atgaataagg ttggcattcc agacgcagat agacgtttta atgaataccc
    17401 attccaatat tctggaggaa tgcgtcaacg tatcgttatt gcgattgccc ttgcctgccg
    17461 acctgatgtc ttgatctgtg atgagccaac aactgccttg gatgtaacta ttcaagctca
    17521 gattattgat ttgctaaaat ctttacaaaa cgagtatcat ttcacaacaa tctttattac
    17581 ccacgacctt ggtgtggtgg caagtattgc ggataaggta gcggttatgt atgcaggaga
    17641 aatcgttgag tatggaacgg ttgaggaagt cttctatgac cctcgccatc catatacatg
    17701 gagtctcttg tctagcttgc ctcagcttgc tgatgataaa ggggatcttt actcaatccc
    17761 aggaacacct ccgtcacttt atactgacct gaaaggggat gcttttgcct tgcgttctga
    17821 ctacgcaatg cagattgact tcgaacaaaa agctcctcaa ttctcagtat cagagacaca
    17881 ttgggctaaa acttggcttc ttcatgagga tgctccaaaa gtagaaaaac cagctgtgat
    17941 tgcaaatctc catgataaga tccgtgaaaa aatgggattt gcccatctgg ctgactagga
    18001 ggaaggaaat gtctgaaaaa ttagtagaaa tcaaagattt agaaatttcc ttcggtgaag
    18061 gaagtaagaa gtttgtcgcg gttaaaaatg ctaacttctt tatcaacaag ggagaaactt
    18121 tctcgcttgt aggtgagtcc ggtagtggga aaacaactat tggtcgtgct atcatcggtc
    18181 taaatgatac aagtaatgga gatatcattt ttgatggtca aaagattaat ggtaagaaat
    18241 cgcgtgaaca agctgcggaa ttgattcgtc gaatccagat gattttccaa gaccctgccg
    18301 caagtttgaa tgaacgtgcg actgttgatt atattatttc tgaaggtctt tacaatcacc
    18361 gtttatttaa ggatgaagaa gaacgtaaag agaaagttca aaatattatc cgtgaagtag
    18421 gtcttcttgc tgagcacttg actcgttacc ctcatgaatt ctcaggcggt caacgtcaac
    18481 gtatcggtat tgcccgtgcc ttggtcatgc aaccagactt tgttattgca gatgagccaa
    18541 tttcagcctt ggacgtttct gtacgtgccc aagtcttgaa cttgctcaaa aaattccaaa
    18601 aagagctcgg cttgacctat ctcttcatcg cccatgactt gtcggttgtt cgctttattt
    18661 cagatcgtat cgcagttatt tacaagggtg ttattgtaga ggttgcagaa acagaagaat
    18721 tgtttaacaa tccaattcac ccatatactc aagccttgct ttcagcggta ccaatcccag
    18781 atccaatctt ggaacgtaag aaggtcttga aggtttacga cccaagtcaa cacgactatg
    18841 agactgataa gccatctatg gtagaaatcc gtccaggtca ctatgtttgg gcgaaccaag
    18901 ccgaattagc acgttatcaa aaaggactaa actaataatg gttttataat ttccatgtca
    18961 atttttatgg aaattataaa actttttttg ttggactgat ttgaaaaact tcgaaagctt
    19021 cctgaaagaa ttttcgaaaa attttaaaaa attctgaaat attattgaca ggtgatgaaa
    19081 aaggtggtag gataggaagc atcaaaaaag aaagcagaaa aagaaatgaa taagagacaa
    19141 gccgtaacta tgaatcaaaa cttttacttt agctacttta gatagtgttt gtagacatgt
    19201 caccatggat gcaaacagtt caagcaattt gtttgtatct atgtggctaa gatttttgat
    19261 tgtggtccat atagatgaat atctaaatgg actagctaag ttgtaacttg ttaggttatt
    19321 tcaagtatcc gtttagatag tatctaggcg gttttttgtt ttatcttttc tgagaaggag
    19381 tttcattatg aaagttgttc gaaaatcact agccccctct tggtagtggg gctcctcttg
    19441 acttctctga tcagtttgca tcagttgaag gca
//
LOCUS       DAVGJL010000021        19084 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.021, whole genome shotgun sequence.
ACCESSION   DAVGJL010000021 DAVGJL010000000
VERSION     DAVGJL010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19084
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.021"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            15..206
                     /gene="secG"
                     /locus_tag="VVE42_000689"
     CDS             15..206
                     /gene="secG"
                     /locus_tag="VVE42_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001828639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEU3072915.1"
                     /translation="MIVIAIFMQPTKNQSSNVFDASSGDLFERSKARGFEAVMQRLTG
                     ILVFFWLAIALALTVLSSR"
     gene            308..2662
                     /gene="rnr"
                     /locus_tag="VVE42_000690"
     CDS             308..2662
                     /gene="rnr"
                     /locus_tag="VVE42_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEU3072916.1"
                     /translation="MKDRIKEYLQDKGKVTVNDLAQALGKDSSKDFRELIKTLSLMER
                     KHQIRFEEDGSLTLEIKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAI
                     DGDTVEVVIKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNQ
                     KISQPIYVKKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVL
                     ESMDIVSEFPEAVVKEAESVPDAPSQKDMEGRLDLRDEITFTIDGADAKDLDDAVHIK
                     ALKNGNLELGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSL
                     NPQVDRLTQSAIMEIDKHGRVVNYTITQTVIKTSFRMTYSDANDILAGDEEKRKKYHK
                     IVPSIELMAKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIE
                     SFMLMANETVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLRIYGTASEISQE
                     ALQDIMRAVEGEPYADVLSMMLLRSMQQARYSEHNHGHYGLAADYYTHFTSPIRRYPD
                     LLVHRMIRDYGCSKEIAEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYV
                     GEEYDAVVSSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVG
                     QQIRIRVERADKMTGEIDFSFVPSEFDVIEKGLKQSSRSGRGRGSNRRSDKKEDKRKL
                     GRSNDKRKHSQKDKKKKGKKPFYKEVAKKGAKHGKGRGKGRRTK"
     gene            2625..3092
                     /gene="smpB"
                     /locus_tag="VVE42_000691"
     CDS             2625..3092
                     /gene="smpB"
                     /locus_tag="VVE42_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEU3072917.1"
                     /translation="MAKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NLKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKKQIQKLEQETKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            3108..3968
                     /gene="tehB"
                     /locus_tag="VVE42_000692"
     CDS             3108..3968
                     /gene="tehB"
                     /locus_tag="VVE42_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEU3072918.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEAVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTNPVHSEVLEAMQTVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSI
                     VEQEDLDMPVGLYDINSASIEQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
     gene            4041..4994
                     /gene="coiA"
                     /locus_tag="VVE42_000693"
     CDS             4041..4994
                     /gene="coiA"
                     /locus_tag="VVE42_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEU3072919.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQLHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHLANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGF
                     YVWELDKEKQVLRLKYLIYQDLRGKLHYQIKEFSYGQGSLLEILRLPYKRQKISHFTV
                     SEDKDICRYIRQQLYYQNLFWMKEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIE
                     QDLTSYYQHFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE"
     gene            5013..6815
                     /gene="pepF"
                     /locus_tag="VVE42_000694"
     CDS             5013..6815
                     /gene="pepF"
                     /locus_tag="VVE42_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEU3072920.1"
                     /translation="MVLQRNEINEKDTWDLSTIYPTDQAWEEALKDLTEQLETVAQYE
                     GHLLDSADNLLEITEFSLEMERQMEKLYVYAHMKNDQDTREAKYQEYYAKAMTLYSQL
                     DQAFSFYDPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLKGKDHVLSQREEELLAGA
                     GEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGTYTRLMESKKREVRRGAYQAL
                     YATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAAVRK
                     HLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYSFTYQEALKKAEDALAVLGEDYL
                     SRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETGHSM
                     HSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLDGFR
                     GTVFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYADLNQEYYGLSKEDNPEIQYEWA
                     RIPHFYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLQAGKSDYPLNVMRKA
                     GVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            6817..7530
                     /locus_tag="VVE42_000695"
     CDS             6817..7530
                     /locus_tag="VVE42_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEU3072921.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGFLKELEDFA
                     RKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQKLFDATLDNPELTATLVPLG
                     DGILMLRKNVADVQLSESE"
     gene            7597..8538
                     /gene="prsA"
                     /locus_tag="VVE42_000696"
     CDS             7597..8538
                     /gene="prsA"
                     /locus_tag="VVE42_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEU3072922.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDVITEHQFY
                     EQVKNNPSAQQVLLNMTIQKVFEKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIIG
                     KELQAANIKVKDQAFQNIFTQYIGGGDSSSSSSTSNE"
     gene            complement(8655..8996)
                     /locus_tag="VVE42_000697"
                     /pseudo
     CDS             complement(8655..8996)
                     /locus_tag="VVE42_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            9408..9503
                     /locus_tag="VVE42_000698"
                     /pseudo
     CDS             9408..9503
                     /locus_tag="VVE42_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(9756..10376)
                     /locus_tag="VVE42_000699"
     CDS             complement(9756..10376)
                     /locus_tag="VVE42_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU3072923.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQGASGDSPLLPGSIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLHTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPAERILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTLNTWNDTSYQS"
     gene            complement(10376..10858)
                     /locus_tag="VVE42_000700"
     CDS             complement(10376..10858)
                     /locus_tag="VVE42_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEU3072924.1"
                     /translation="MAKKVKIKKTLVEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNYHIFIDKIALDLDRMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            complement(10858..11700)
                     /locus_tag="VVE42_000701"
     CDS             complement(10858..11700)
                     /locus_tag="VVE42_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEU3072925.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGVLAAILGIYERMIGFLAH
                     PFKDLKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLAWLWTTFIISGLGLYALNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDLLGTFFPIGIGAGATLIVFSKLMDYALNNYHSR
                     VYHFIIGIVLSSTLLILIPNAGNTESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            complement(11795..12583)
                     /locus_tag="VVE42_000702"
     CDS             complement(11795..12583)
                     /locus_tag="VVE42_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEU3072926.1"
                     /translation="MRKQIHPLILFSFFGIMMFILIINRPLNDSQSFKTKSNIAQALK
                     HLDKPIIDLSGWQRPEEINYDALSQNISGAIVRVHSGAQTTKENDASFINGIDKAYKS
                     HITELQKRNVPVAVYAYVAGKSVQEMEKAAEVFYNAASPYSPSYYWLDVEDKTMSNMN
                     EGVENFRAKLASLGAKNIGIYVGVYFMEEHSIDTGKFTSVWIPSYGSDSGFLESSPKT
                     DLDYDIHQYTSKGKIAGFDHDLDINVISPLKNKEETFRKLFLKP"
     gene            12727..14106
                     /gene="glmU"
                     /locus_tag="VVE42_000703"
     CDS             12727..14106
                     /gene="glmU"
                     /locus_tag="VVE42_000703"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEU3072927.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLAGQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRMALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDVEIASEVQIEA
                     NVTLKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVTVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGDNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPKNQ"
     gene            14116..14661
                     /locus_tag="VVE42_000704"
     CDS             14116..14661
                     /locus_tag="VVE42_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU3072928.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTAQRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELQKK"
     gene            14673..14987
                     /locus_tag="VVE42_000705"
     CDS             14673..14987
                     /locus_tag="VVE42_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HEU3072929.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKDHGFSQETLKIQVEPSIHKSRRIENTKRNVFNSKLNKILFAVIFLLILLVLAMK
                     LL"
     gene            15004..15696
                     /locus_tag="VVE42_000706"
     CDS             15004..15696
                     /locus_tag="VVE42_000706"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEU3072930.1"
                     /translation="MKIGIIAAMPEELAYLVQHLDNAQEQVVLGNTYHTGTIASHEVV
                     LVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYAYGQMAQQPLYFESDKTFVAQIQESLSQLDQNWHLGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHALNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLTFLKALD"
     gene            15773..16264
                     /gene="rocS"
                     /locus_tag="VVE42_000707"
     CDS             15773..16264
                     /gene="rocS"
                     /locus_tag="VVE42_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HEU3072931.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            16362..16952
                     /locus_tag="VVE42_000708"
     CDS             16362..16952
                     /locus_tag="VVE42_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEU3072932.1"
                     /translation="MERLEDYIAFDLEFNQHEGQTHLIQVSAVRFRDGREIDAYDSYV
                     HTSAPLKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIGYNAAKSDLPILAE
                     HGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGGLDLSQLFD"
     gene            16961..18724
                     /locus_tag="VVE42_000709"
     CDS             16961..18724
                     /locus_tag="VVE42_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEU3072933.1"
                     /translation="MKPEKPKKLGLKQIYLNLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINLLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFGFMKKLTCAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     EFIQAYLEDKYWMWWLSILLLSLIFLYVSVRLMFALPKIVYDQLTVREAVMFSLEKTK
                     KRVTFYAWNLFYILLKAHLLFYLPLIPLLLAQTLVDDITQRESLILGILNFVVIKNLY
                     YMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVMTCASLFFALEGYIYLEAPMVH
                     LPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANLKNLA
                     GINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKKDSPD
                     MMNRFLARYAAKLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTRATGY
                     TMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLHLGVDGVITDDLEKVQREIEV
                     AQEDPEYTDLLLKKALEFFEF"
     gene            18938..>19084
                     /locus_tag="VVE42_000710"
     CDS             18938..>19084
                     /locus_tag="VVE42_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU3072934.1"
                     /translation="MAYSIDFRKKVLSYCERIGSITEASHVFQISRNTIYGWLKLKEK
                     TGELN"
BASE COUNT         6017 a         3404 c         4103 g         5560 t
ORIGIN      
        1 tagtattatc tgttgtgatt gtgattgcaa ttttcatgca accaaccaaa aaccaatcca
       61 gcaatgtatt tgatgccagt tcaggtgatt tgtttgaacg cagtaaagct cgcggttttg
      121 aagctgtaat gcagcgtttg acagggattt tagtcttttt ctggctagcc attgccttag
      181 cattgacggt attatcaagt agataagaaa ataatgggca ggactaggtc tttgcctctt
      241 tttattttta aaggatgttt gagaaggttt tacagtaaaa gaaaattaaa aaatctagaa
      301 agaaaatatg aaagatagaa taaaagaata tttacaagac aagggaaagg tgactgttaa
      361 tgatttggct caggctttgg gaaaagacag ttccaaggat tttcgtgagt tgattaaaac
      421 cttgtcctta atggaaagaa agcaccaaat tcgttttgaa gaagatggta gtctgacatt
      481 agaaattaag aaaaaacatg agattaccct caaggggatt tttcatgccc ataaaaatgg
      541 ctttggcttt gttagtctgg aaggcgagga ggacgacctt tttgtaggga aaaatgatgt
      601 caactatgct attgatggtg ataccgtcga ggtagtgatt aagaaagtcg ctgaccgcaa
      661 taagggaaca gcagcagaag ccaaaattat tgatatccta gaacacagtt tgacaacagt
      721 tgtcgggcaa atcgttctgg atcaggaaaa gcctaagtat gctggctata ttcgttcaaa
      781 aaatcagaaa atcagtcaac cgatttatgt taagaaacca gccctaaagt tagaaggaac
      841 agaagttctc aaggtcttta tcgataaata cccaagcaag aaacatgatt tctttgtcgc
      901 gagtgttctc gatgtggtgg gacactcaac ggatgtcgga attgatgttc ttgaggtttt
      961 ggaatcaatg gacattgtat ccgagtttcc agaagctgtt gttaaggaag cagaaagtgt
     1021 gcctgatgct ccgtctcaaa aggatatgga aggtcgtctg gatctaagag atgaaattac
     1081 ctttaccatt gacggtgcgg atgccaagga cttggacgat gcagtgcata tcaaggctct
     1141 gaaaaatggc aatctggagc ttggggttca catcgcagat gtttcttatt atgtgaccga
     1201 ggggtctgcc cttgacaagg aagcccttaa ccgtgcgact tctgtttacg tgacagaccg
     1261 agtggtgcca atgcttccag aacgactatc aaatggcatc tgctctctca atccccaagt
     1321 tgaccgcctg acccagtctg ctattatgga gattgataaa catggtcgtg tggtcaacta
     1381 taccattaca caaacagtta tcaagaccag ttttcgtatg acctatagcg atgccaatga
     1441 tatcctagct ggcgatgaag aaaagagaaa aaaatatcat aaaattgtac caagtatcga
     1501 actcatggcc aagcttcatg aaactttaga aaacatgcgt gtgaaacgtg gagctctcaa
     1561 ttttgatacc aatgaagcga agattttagt ggataaacaa ggtaagcctg ttgatatcgt
     1621 tcttcggcag cgtggtattg ccgagcggat gattgagtct tttatgttga tggctaatga
     1681 aacagttgcc gaacatttca gcaagttgga tttgcctttt atctatcgaa ttcacgagga
     1741 gcctaaggct gaaaaggttc agaagtttat tgattatgct tcgagttttg gcttgcgcat
     1801 ttatgggact gccagtgaga ttagtcagga ggcacttcaa gacatcatgc gtgctgttga
     1861 gggagaacct tatgcagatg tattgtccat gatgcttctt cgctctatgc agcaggctcg
     1921 ttattcggag cacaatcacg gccactatgg actagctgct gactattata ctcactttac
     1981 cagtccaatt cgtcgttatc cagaccttct tgttcaccgt atgattcgag attacggctg
     2041 ttctaaggaa atagcagagc attttgaaca agtgattcca gagattgcga cccagtcttc
     2101 caaccgtgaa cgtcgtgcca tagaagctga gcgtgaagtc gaagccatga aaaaggctga
     2161 gtatatggaa gaatacgtgg gtgaagagta tgatgcagtt gtatcaagta ttgtcaaatt
     2221 cggtctcttt gtcgaattgc caaacacagt tgaaggcttg attcacatca ctaatctacc
     2281 tgaattttat catttcaatg agcgtgattt gactcttcgt ggagaaaaat caggtatcac
     2341 tttccgagtg ggtcagcaga tccgtatccg tgttgaaaga gcggataaaa tgactggaga
     2401 gattgatttt tcattcgtac ctagtgagtt tgatgtgatt gaaaaaggct tgaaacagtc
     2461 tagtcgtagt ggcagagggc gtggttcaaa tcgtcgttcg gataagaagg aagacaagag
     2521 aaaattagga cgctcaaatg ataagcgtaa gcattcacaa aaagacaaga agaaaaaagg
     2581 aaagaaacct ttttacaagg aagtagctaa gaaaggagcc aagcatggca aagggcgagg
     2641 gaaaggtcgt cgcacaaaat aaaaaggcac gccacgacta tacaatcgta gatacgctag
     2701 aggcagggat ggtcctgact ggaactgaaa tcaagagtgt acgagctgct cgaattaatc
     2761 tcaaggatgg ctttgctcaa gtgaaaaatg gagaagtttg gctgagcaat gttcatatcg
     2821 cgccttacga agagggcaat atctggaacc aggaaccaga acgtcgtcgt aaactcctgc
     2881 tccataaaaa gcaaattcaa aaattggaac aagagaccaa agggacagga atgaccttag
     2941 ttccccttaa ggtctatata aaagatggct acgctaagct tcttttagga cttgccaaag
     3001 ggaagcatga ctatgacaaa cgggagtcta tcaaacgtcg tgagcaaaat cgagatatcg
     3061 cgcgtgtgat gaaagctgtt aatcagcgat aaaaagagga attgaaaatg gaaaaattag
     3121 ttgcctataa acgcatgcct ttgtggaata aacaaacaat gcctgaagct gttcagcaaa
     3181 agcacaatac aaaagttggg acttggggga aaattactgt cttgaaggga gctctcaagt
     3241 ttattgaatt gacagaagaa ggggaagttc tagctgaaca cctctttgaa gcaggggcag
     3301 acaatccaat ggcccaacct caagcctggc accgagtgga agctgccaca gatgatgtgg
     3361 aatggtactt ggaattttat tgtaaacctg aggattattt tgctaaaaaa tacaatacca
     3421 atcctgttca ttcagaggtc ctagaggcca tgcagacagt gaaacaaggg aaagctttgg
     3481 atttgggttg tggtcagggg cgtaattctc tttttctagc ccagcaagat tttgatgtga
     3541 cggctgtaga tcaaaatgga ctagctcttg aaatcttgca aagcattgtg gagcaggaag
     3601 atttggacat gcctgttggc ctttacgata tcaattcagc tagcattgaa caagaatatg
     3661 attttatcgt ttcaacagtt gttctcatgt ttctacaagc ggaccgcatt ccagctatta
     3721 ttcaaaatat gcaggagaaa accagtgttg gtggttacaa ccttatcgtt tgtgccatgg
     3781 acacggagga ttatccttgc tcggttaact tcccattcac ctttaaagaa ggagaactgg
     3841 cagactatta caaggattgg gaattggtta agtacaatga aaatccaggc catttgcatc
     3901 gtcgcgatga gaatggcaat cgtattcaac tacgctttgc gaccttacta gctaagaaaa
     3961 tcaagtaaac acacatgaag attaggaatt ttcctgatct tttttctttt ttacgaatga
     4021 tatagaaaag gagggaattc atgtttgttg cgagagatgc taggggagaa ttggtaaatg
     4081 tgttagagga taaacttgag aagcaagcat acacctgccc agcttgtgga ggccagctcc
     4141 atttgcgtca aggaccaagt gtacggacgc attttgccca taaatcctta aaagactgtg
     4201 attttttctt tgaaaatgaa agtccagaac acctggccaa taaggaatcc ctctatcact
     4261 ggttgaaaaa agagacaaag gttcaattag agtacccgct ttcagaactt aaacagattg
     4321 cggatgtatt tgtaaatggc aatctagctc tagaagttca gtgtagtccc ttgcctcaga
     4381 aagtccttaa agagcgaagt gagggatatc gtagtcaggg ttaccaagta ctgtggttgc
     4441 tgggtcaaaa actgtggctc aaggagcgtt tgactcgtct acagcaaggt tttctttatt
     4501 tcagtcaaaa catgggcttt tatgtttggg aattagacaa ggaaaaacaa gttttaagac
     4561 tcaaatacct gatttaccag gatctccgcg gtaaactcca ttatcaaatc aaggaatttt
     4621 cctatggtca aggtagttta ttggaaatat tgcgtcttcc ctataagaga caaaaaatat
     4681 ctcattttac agtttctgag gacaaggaca tctgtcgcta tatccggcaa caactttatt
     4741 atcaaaatct cttttggatg aaagaacaag cagaagccta tcaaaaggga gaaaatatcc
     4801 tgacttatgg actgaaagaa tggtatccac aaattcgacc aatagtgggc aaatttttcc
     4861 agattgaaca agacttgact agctattatc agcactttta tacctattac caaaaaaatc
     4921 ctcaaaatga ttggcaaaag ctttatccac cagcctttta tcagcaatat ttcttgaaaa
     4981 atatggtaga atagaaagga tggaggaatc taatggtatt acaaagaaat gaaataaatg
     5041 aaaaagatac atgggatcta tcaacgatct acccaactga ccaggcttgg gaagaagcct
     5101 taaaagattt aacagaacaa ttggagacag tagcccagta tgaaggccat ctcttggata
     5161 gtgcggataa cctactagaa atcactgaat tttctcttga aatggaacgc cagatggaga
     5221 agctttacgt ttatgctcat atgaagaatg accaggatac acgtgaagct aagtatcaag
     5281 agtactatgc caaggccatg acactctaca gccagttaga ccaagccttt tcattctatg
     5341 atcctgaatt tatggagatt agcgaaaagc agtatgctga ctttttagaa gctcaaccaa
     5401 agctgcaggt ttatcaacac tattttgaca agctcttgaa aggcaaggat cacgttcttt
     5461 cacaacgtga agaagaatta ttggctggag ctggagaaat ctttggttca gcaagtgaaa
     5521 ccttcgctat cttggacaat gcggatattg tgttccctta tgtcctagac gatgatggta
     5581 aagaagttca gctatctcat gggacttaca cacgtttgat ggagtctaaa aaacgtgagg
     5641 ttcgccgtgg tgcctatcaa gctctttatg cgacttacga acaattccaa cacacctatg
     5701 ccaaaacctt gcaaaccaat gttaaggtgc aaaattaccg tgctaaagtt cgtaactaca
     5761 agagtgctcg tcatgcagcc ctcgcagcga attttgttcc agaaagtgtt tatgacaatt
     5821 tggtagcagc agttcgcaag catttgccac tcttacatcg ctatcttgag cttcgttcaa
     5881 aaatcttggg gatttcagat ctcaagatgt acgatgtcta cacaccgctt tcatctgttg
     5941 aatacagttt tacctaccaa gaagccttga aaaaagcaga agatgctttg gcagtcttgg
     6001 gtgaggatta cttgagccgt gttaaacgtg ccttcagcga gcgttggatt gatgtttacg
     6061 aaaatcaagg caagcgttca ggtgcctact ctggtggttc ttacgatacc aatgccttta
     6121 tgcttctcaa ctggcaagac aatctggaca atctctttac tcttgttcat gaaacaggtc
     6181 acagtatgca ttcaagctat actcgtgaaa ctcagcctta tgtttacggg gattactcta
     6241 tctttttggc tgagattgcc tcaactacca atgaaaatat cttgacggag aaattattgg
     6301 aagaagtgga agacgacgca acacgctttg ctattctcaa taacttccta gatggtttcc
     6361 gtggaacagt tttccgccaa actcaatttg ctgagtttga acacgccatt caccaagcag
     6421 atcaaaatgg ggaggtcttg acaagcgatt tcctaaataa actctacgca gacttgaacc
     6481 aagagtatta tggtttgagt aaggaagaca atcctgaaat ccaatacgag tgggctcgca
     6541 ttccacactt ctactataac tactatgtat atcaatattc aactggcttt gcggccgcct
     6601 cagccttggc tgaaaaaatt gtccatggta gtcaagaaga ccgtgaccgc tatatcgact
     6661 acctccaggc aggtaagtcg gactatccac ttaatgtcat gagaaaagct ggtgttgata
     6721 tggagaagga agactacctc aacgatgcct ttgcagtctt tgaacgccgt ttaaatgagt
     6781 ttgaagccct tgttgaaaaa ttaggattgg cataaaatgg ttgaatcgta tagtaagaat
     6841 gctaaccata acatgcgtcg tcctgtcgtc aaagaagaaa ttgtagactt gatgcgtcag
     6901 cgtcaaaagc aggtcacagg tttcttgaaa gaattggaag actttgcccg caaggaaaat
     6961 attcctatta ttccccatga aacggttgct tatttccgtt ttcttatgga aaccatgcag
     7021 cctaaaaata ttctggaaat tgggacggct atcggttttt cagctctctt gatggctgaa
     7081 catgcgccaa atgctaagat tacaactatt gatcgtaatc cagaaatgat tggttttgcc
     7141 aaggaaaatt ttgcccagtt tgacagtcgc aagcaaatca ctctcctaga gggagatgcg
     7201 gtggatgtct tatctacact gacagagtct tatgatttcg tctttatgga ttctgccaag
     7261 tctaaataca tcgtctttct gccagaaatc ctcaaacatt tggaagttgg tggtgtggtt
     7321 gtcttggatg atatttttca aggtggtgat gttgccaagg atattatgga agtccgtcgt
     7381 ggtcagcgaa ccatttatcg aggccttcaa aaattatttg atgcaacctt agacaatcca
     7441 gaactcaccg caacattagt gcctttagga gatggtattc tcatgcttcg taaaaatgta
     7501 gcagatgttc aactgtctga aagcgaatga ttttcagaaa aatttaagaa aaaatagtaa
     7561 aatagataga gtaacactta tctcaaagga gtagacatga agaaaaaatt attggcaggt
     7621 gccatcacac tattatcagt agcaacttta gcagcttgtt cgaaagggtc agaaggggca
     7681 gaccttatca gcatgaaagg ggatgtcatc acagaacatc aattttatga gcaagtgaaa
     7741 aacaaccctt cagcccaaca agtcttgtta aatatgacca tccaaaaagt ttttgaaaaa
     7801 caatatggct cagagcttga tgataaagag gttgatgata ctattgccga agaaaaaaaa
     7861 caatatggcg aaaactacca acgtgtcttg tcacaagcag gtatgactct tgaaacacgt
     7921 aaagctcaaa ttcgtacaag taaattagtt gagttggcag ttaagaaggt agcagaagct
     7981 gaattgacag atgaagccta taagaaagca tttgatgagt acactccaga tgtaacggct
     8041 caaatcatcc gtcttaataa tgaagataag gccaaagaag ttctcgaaaa agccaaggca
     8101 gaaggtgctg attttgctca attagccaaa gataattcaa ctgatgaaaa aacaaaagaa
     8161 aatggtggag aaattacctt tgattctgct tcaacagaag tacctgagca agtcaaaaaa
     8221 gccgctttcg ctttagatgt ggatggtgtt tctgatgtga ttacagcaac tggcacacaa
     8281 gcctacagta gccaatatta cattgtaaaa ctcactaaga aaacagaaaa atcatctaat
     8341 attgatgact acaaagaaaa attaaaaact gttatcttga ctcaaaaaca aaatgattca
     8401 acatttgttc aaagcattat cggaaaagaa ttgcaagcgg ccaatatcaa ggttaaggac
     8461 caagccttcc aaaatatctt tacccaatat atcggtggtg gagattcaag ctcaagcagt
     8521 agtacatcaa acgaatagtc caaatcaatg agtcagggaa aaaaactcga cttcaggaaa
     8581 aaatgaagca aacattccca caataaaacg catagtacaa ggtttgtact gaccccaaaa
     8641 gttagacaat taatttatct gaaggattta gttctgtatt gcacagggct aaatcctttt
     8701 agttttacct taattctctt attgttgtaa taatcaatat agtctataat ggcttgttcc
     8761 aattgattaa gtgatttaaa tgttttctca tagccataaa acatttcgga ttttaaaatg
     8821 ccaaagaaag attccatcct accgttgtct tggctgtttc ctttgcgtga catagatgct
     8881 tgaattccct tactctctag gaagctatga taagaatcgt gttggtattg ccagccttgg
     8941 tcactatgga gaatcgtatt ctcgtagtgc ttctctttga atgcctgttc caacattaac
     9001 gatcaatcaa tttaatcatg tacctaatat tagaattgtt tatcccaaat ttatttgaaa
     9061 gcttctctaa gctatatcct tgttttctaa gttcatagat ctgaacttta tcatcataag
     9121 ttaatttcat aataaaaaca ccccaaaagt tagatttttt ctgtctaact tttggggtgt
     9181 agttcatgta cacctgatat gatgcgtttt ataattttaa agactttttg accagcctca
     9241 ttttttaact tgatactcag tgaaaagcaa agattaaact aggaagctag ctgtaggctg
     9301 ctcaaagaac agctttgagg ttgcagataa aacttgtgag gtaaccaaca tatataatgt
     9361 gaagctgacg tggtttgaat agattttaga agggtatgag tctggaagtt ttaatggata
     9421 atgcaagatt ccatagaatg ggtaagctag agttcttatg tgaagagttt gggcataaac
     9481 ttttaccttt tcctccctac tcatcttagt atagaaaagt gaatctaaaa tagtacataa
     9541 ctgcttctaa aacattctta taaattgatt taaattctca aatcatatta ttcagttctt
     9601 atttcatttt gctctacaat cctgttgaga agacacgtgt tcatatcaaa aaggtattgg
     9661 caagttgcaa taccttttta caaggttctt ttgtcttatt tttgtttcaa ctgactatat
     9721 ctcctatggt tctagttcag aaggctaggc tataattatg attgataaga agtatcattc
     9781 caagtattga gagtgaatgt ttcaaaatca tgggtttcta taatggtcag gctggcattt
     9841 gctagaccgc catctttacg aagaagtggt tctttatagc ctaggagagt acgaagactg
     9901 gcagtaagat tggcgccgtg tccgacaatt agaatacgct cagctggact atcttttaat
     9961 gatttgataa attggatggt ccgctgagtt gtactataga gggattcggc tccgaacatt
    10021 cgagtgtcaa attgagcaag atttgaacga aaagcctgga tttgttgcgg gtaaatagct
    10081 tccagggttg caattttcaa accttctaac ttcccaagtt gccattcacg gagattagga
    10141 acgatttcta aagaacaggg ggtatggagt tgactttgga taatctcagc agatttgacc
    10201 gctcgaggta aatcacttga ataaatctga tcaaaaggaa tttccttgag atactggcca
    10261 agtcgtttta gggtttcaat ggatccagga agaagaggag aatcaccgct agcaccttga
    10321 aaacgacctt cttggttcca gagggtacga ccgtggcgga caaagtagag tttcattact
    10381 tgatgtcctc caaaatatct acaaagtctg cctttacaaa gctagccaag tcttgtggcg
    10441 cgacgataat gctgtgtccg acttcgcctg cagagacaat cattcgatcc aaatctagag
    10501 caattttatc gataaaaatg tgataattgt gtttctgacg aattccgaca ggattattgg
    10561 ctccatgaat gtaaccagtt gttttttcta agtccttttg tggaatcatg ctcacttttt
    10621 tattgccaga aattttagct agtttctttt cagacaagtg ctgagtgata gggacaattc
    10681 cgataatcgg tccggtcttg tctcccaaaa gcgccaaggt tttgaaaatc tgatctcgtt
    10741 cataaccttg aggaagctct ccttctaggg cattgatttg aatcccctga tgagggatag
    10801 ctgctttaga taggatttgt tccaccaatg tttttttgat tttaactttt tttgccatta
    10861 tttatattta tcctccaatt gactcatcca aataccaagc cagattccca gcgcaaagaa
    10921 gaaggcgatg atgacataac cgacaagtga aagtcctgtg tattggatac tttcagtgtt
    10981 tcctgcattt ggaattaaga tcaaaagggt acttgatagg acgataccga tgatgaaatg
    11041 atagacgcgt gagtggtagt tgtttaaggc ataatccatc aattttgaaa aaacgatgag
    11101 agttgcacct gcaccaattc caatcggaaa gaaggttccc aagagatcaa aagttttaaa
    11161 accagtcaac ataggagcat agagtcccaa aatcaaaagt aaattcgatg ggctgaggcc
    11221 aggaaccaag acgccaaggg ccaatagtgc gcctgctagg acgaagttaa gaaagctggc
    11281 gcttaaggtt ccaacgacaa aatttaaggc atatagtcct aatccagaaa tgataaaggt
    11341 tgtccataac caagctaaat caatcttgtc tcggtcagat tctcgagttg attctttgag
    11401 gaggctagga actgtaccga taatagctcc cgcaaagctc cataatacaa agacctgata
    11461 attttcaagc aggtattcaa tcgggtagga aaataagccg attcccagaa gcataccgat
    11521 ggcaactgga ataaagtaca aaacattttc tttaaggtct ttaaagggat gggccagaaa
    11581 gccaatcatt cgttcataga ttcctaagat tgctgctaga acccctccgg aaattcccgg
    11641 taggataaat ccaagagcaa ttacaatccc tttaataacg cgtgctaacc atgagagcat
    11701 atatttcctc caactatttc tatttcaaaa aatccttact atattgtatc acaatcagac
    11761 acaaaaagaa aaagcaaatg ataaacaaat gcttttaagg ttttaaaaaa agttttcgaa
    11821 aggtttcttc tttatttttt aagggagaga taacgttgat atctaaatcg tggtcaaagc
    11881 cggcaatttt tcctttagat gtgtattggt gaatatcata atctaaatca gttttaggac
    11941 tgctctccaa aaatcctgag tctgagccgt aggacggaat ccaaacagag gtaaacttgc
    12001 ctgtatcaat actgtgttct tccatgaagt agacaccaac gtagatgccg atgtttttag
    12061 cacctagtga tgctagtttt gctcgaaagt tttcgacacc ttcgttcata ttagacatgg
    12121 ttttgtcttc cacgtcaagc caatagtaac tagggctgta aggagaggca gcattgtaga
    12181 aaacttcggc agccttttcc atttcttgga cactttttcc agctacataa gcgtagacag
    12241 caactgggac attccgcttt tgaagttcag tgatatgact cttataggcc ttgtctattc
    12301 cattgataaa tgaagcatca ttttcttttg ttgtttgagc accactgtga acacgaacaa
    12361 tagcacctga aatattttgt gagagggcat cgtagttgat ttcctcagga cgctgccagc
    12421 cagagaggtc aataatcggt ttgtctaagt gtttcaaagc ctgtgctata ttggattttg
    12481 ttttaaacga ttggctgtca ttaagtgggc gattgatgat taaaatgaac atcataatcc
    12541 caaaaaaact aaataaaata agtggatgaa tttgttttct catatcttat aagtctaccc
    12601 taaaaatcaa aaaaaatcaa aaaaatgggt taaggaagag actttagagc attttttcat
    12661 tcaagagtgc ggaatgattt gaaatatggt ataataaaag ggaatttcta cagaaaagag
    12721 aagattatgt caaattttgc cattatttta gcagcgggta aagggactcg catgaaatct
    12781 gatttgccaa aagttttgca caaggttgcg ggtatttcta tgttggaaca tgttttccgt
    12841 agtgtgggag ctatccaacc tgaaaagaca gtaacagttg taggacacaa ggcagaattg
    12901 gttgaggagg tcttggctgg acagacagaa tttgtgactc aatctgaaca gttgggaact
    12961 ggtcatgcag ttatgatgac agaacctatc ttagaaggtt tgtcaggaca caccttggtc
    13021 attgcaggag atactccttt aatcactggt gaaagcttga aaaacttgat tgatttccat
    13081 atcaatcata aaaatgtggc cactatcttg actgctgaaa cggataatcc ttttggctat
    13141 ggacgaattg ttcgtaatga caatgctgag gttcttcgta ttgttgagca gaaggatgct
    13201 acagattttg aaaagcaaat caaggaaatc aacactggaa catacgtctt tgacaacgag
    13261 cgtttgtttg aggctttgaa aaatatcaat accaataacg ctcaaggcga atactatatt
    13321 acagacgtca ttggtatttt ccgtgaaact ggtgaaaaag ttggcgctta tactttgaaa
    13381 gattttgatg aaagtcttgg ggtaaatgac cgtatggcgc ttgcgacagc tgagtcagtt
    13441 atgcgtcgtc gcatcaatca taaacacatg gtcaacggtg ttagctttgt caatccagaa
    13501 gcaacttata tcgatattga tgttgagatt gcttcggaag ttcaaatcga agccaatgtt
    13561 accttgaaag ggcaaacgaa aattggtgct gagactgttt tgacaaacgg tacttatgta
    13621 gtggacagca ctatcggagc aggagcggtc attaccaatt ctatgattga ggaaagtagt
    13681 gttgcagacg gtgtgacagt cggtccttat gctcacattc gtccaaattc aagtctgggt
    13741 gcccaagttc atattggtaa ctttgttgag gtgaaaggat cttcaatcgg tgagaatacc
    13801 aaggctggtc atttgactta tatcggaaac tgtgaagtgg gaagcaacgt taatttcggt
    13861 gctggaacta ttacagtcaa ctatgacggc aaaaacaaat acaagacagt cattggagac
    13921 aatgtctttg ttggttcaaa ttcaaccatt attgcaccag tagaacttgg tgacaattcc
    13981 ctcgttggtg ctggttcaac tattactaaa gacgtgccag cagatgctat tgctattggt
    14041 cgcggtcgtc agatcaataa agacgaatat gcaacacgtc ttcctcatca tcctaagaac
    14101 cagtaggagc ctatcatgga gtttgaagaa aaaacgctta gccgaaaaga aatctatcaa
    14161 ggaccaatat ttaaactggt ccaagatcag gttgaattac cagaaggcaa gggaactgcc
    14221 caacgggatt tgattttcca caatggggct gtctgtgttt tagcagtaac ggatgaacaa
    14281 aaacttatct tggtcaagca gtaccgcaaa gctatcgagg ctgtctctta cgaaattcca
    14341 gccggaaaat tggaagtagg agaaaacaca gcccctgtgg cagctgccct tcgtgaatta
    14401 gaggaagaaa cagcctatac agggaaatta gaactcttgt acgattttta ttcagctatt
    14461 ggcttttgta atgagaagtt aaaactatat ttagcaagcg atttaacaaa agtggaaaat
    14521 ccgcgtccgc aggatgagga tgaaaccttg gaagtccttg aagtgagctt agaagaagcg
    14581 aaagaattaa tccaatcagg tcatatttgt gatgccaaga caattatggc tgttcagtat
    14641 tgggagttgc agaaaaaata gaggaggtca gtatgggtaa atctttatta acggatgaaa
    14701 tgattgaaag agctaataga ggcgaaaaaa tttcaggtcc tcctttgcta gatgataatg
    14761 aggaaactaa gattttacca acctcttctt cccgttttgg ttatgccaat cctaaggatc
    14821 atggttttag ccaggaaacc ttgaagattc aggtcgaacc atctattcat aaaagccgtc
    14881 gtattgaaaa taccaagaga aatgtcttca attctaagtt gaataaaatc ttattcgcgg
    14941 tcatctttct cttgattttg cttgttttag caatgaaact tttgtaatag aaaaggaatt
    15001 gaaatgaaaa taggaattat tgctgctatg ccagaagaac tggcttatct ggtccagcat
    15061 ttagacaatg cccaggagca agttgttttg gggaatacct atcatacagg aaccattgct
    15121 tctcatgaag tcgttcttgt agaaagtgga attggtaagg tcatgtctgc tatgagtgtg
    15181 gcgattttgg ctgatcattt ccaggtggat gcccttatta atacgggttc agctggggca
    15241 gtagcagaag gtatcgctgt tggggatgtc gtgattgctg acaaattagc ctatcatgac
    15301 gtggatgtca cagcttttgg ctatgcttat ggacaaatgg cgcaacaacc gctttatttc
    15361 gaatcagaca aaacctttgt tgctcaaatc caagagagtt tatctcaatt ggaccaaaac
    15421 tggcatcttg gtttgattgc tacaggagat agttttgttg caggaaatga caagatagaa
    15481 gcgattaagt cccatttccc agaagtttta gccgtggaga tggagggggc agctattgct
    15541 caagcagcgc atgccctcaa tctcccagtc ttagtcatcc gagctatgag tgacaatgcc
    15601 aaccatgaag caaacatctt ttttgatgag tttattatcg aagctggacg tcgctctgcc
    15661 caagtcttgt tgaccttttt gaaggcttta gattaagcgg aaatttgaca gtttttctag
    15721 cttatgataa gatttaagta aagaaaagct agaaaacgtt tcagaggata ttatgagtat
    15781 tgaaatgacc gtcagtgaga ttgcagaggt cttaggatta tctcgccaag caatcaataa
    15841 ccgtgtcaaa gaattaccag aagaagacac agataaaaat gacaaagggg taacagttgt
    15901 taccagaagt ggcttgatta agctagaaga aatctataaa aaaacgattt ttgaagatga
    15961 gcctgtcagt gaagatgtca aacaacgtga actgatggag attctagtgg atgagaagaa
    16021 tgcagaaatt cttcgtcttt atgaacaatt aaaagccaag gatcgtcagt tatcagaaaa
    16081 agacgagcag atgcgtatca aagaccgtca gattgctgag aaagacaaac aattagatca
    16141 gcaacaacaa ttgacccttc aagccatgaa ggatcaagaa aatcttaagc tagagctgga
    16201 ccaagcaaaa gaagaagtcc aatccactaa aaaaggcttt tttgctcgtt tatttggagg
    16261 ataatcaagg agctgtttag gttaaatgct aacctgatgg cttcttttgt ttgtgaatag
    16321 tatagttgat tagaacagag acagtagagg agaggtacag aatggaacga ttagaagatt
    16381 acattgcttt tgatttagaa tttaatcagc acgaaggaca aacacacttg attcaagtat
    16441 cggcagtgcg ttttagagat ggtcgggaaa tcgatgccta tgattcctat gttcatacca
    16501 gcgcccctct aaaaagcttt atcaatggtt tgaccgggat tacagctgag accttaaaag
    16561 atgctcctcc agttgagaaa gtcttagaag aattccaaga gtttgtgggt tctttgctga
    16621 tgataggcta caatgctgct aaaagtgatt tacctatttt agcagagcat ggtttagact
    16681 atagccaaca gtacaaggtg gatttgtatg atgaagcttt tgaacgtcgg agttcagatt
    16741 tacatggtat tgccaatctc aaattgcaaa ctgttgcctc atttcttgga tttcaaggtc
    16801 agtctcataa tagtttagag gatgctcgga tgacggcgcg tgtttacgaa tccttcttag
    16861 agtcggatca agctagggaa ttgttagaga cagaaagtag cttatcaaac aatccttttg
    16921 gtggcttgga cttgtctcaa ttatttgact aggagtgcct atgaaacctg aaaaacctaa
    16981 aaaattgggt cttaaacaga tttacctcaa tcttgataaa atcctctttc tatttttctt
    17041 gatttttatg atagtggagt atttgtgggt accattcaat tcatggttgg ctagcttctt
    17101 attagatcag acaggttatc tttttatttc ctataacaat gtcttagcca tccttactca
    17161 ttctccattg attagtctag cttttttagc cttgattgta attaaccttc tggttgctta
    17221 ttttcaaata ggactcctct ttattggtgc tcgtcatctc ctctataatg aaaagcgaag
    17281 gctaattgag tatatccgta aggttttcca tgaaagtttt ggatttatga aaaagctaac
    17341 ttgcgctaaa gccttgttta tctttttcta tatagctatg ctttttcctt ttatacggaa
    17401 aatgctgaag atttattatc ttaataaaat cgtcattcca gagtttatcc aagcttattt
    17461 agaagataaa tattggatgt ggtggctgag catcctactt ctatctctta tctttctcta
    17521 tgtctctgtc agattgatgt ttgcccttcc gaagattgta tatgatcagc tgactgttcg
    17581 agaagcagtg atgtttagct tggagaaaac caagaagaga gttacttttt atgcttggaa
    17641 tttattttac atcttgctca aagcacattt attattttat cttcctttga ttcctttatt
    17701 attggctcaa actttggtag atgatatcac tcagagagag tctctgattc ttggtatttt
    17761 gaattttgtc gtgattaaaa atctctatta tatggctctg acttattttt tggttaaatt
    17821 tgtttcattt ttgacaggaa aggagctgga tatgcttcct aggagagaaa aagatcacat
    17881 cgtgcgatgg ggtgtcatga cttgtgccag tcttttcttt gccttagaag gttatattta
    17941 tctggaggct cccatggttc atctacctca acttatttct caccgagggg tttccaatgc
    18001 aaatgggatt caaaatacag tagagtcctt ggaaactaca gcacaactca aaccagactt
    18061 ggtggagacg gatgtgcagg aaacaaaaga tgggcagttt gtcatgatgc atgatgctaa
    18121 cttgaaaaat ctagcaggta tcaataaaag tcctcaagac ttaaacttgg aggagcttaa
    18181 agggattgat atttttgaaa atggctacca gactaaaatt tcaagctttg aagattatct
    18241 cagtcgagcc aacgaacttg gtcaaaaatt actaattgaa attaaaacca gtaaaaaaga
    18301 tagtccagac atgatgaacc gctttttagc ccgttatgct gcaaagctca agatttatgg
    18361 acatcaaatc cagtctttag actaccatgt tgtcgaaaaa gtaagacagt atgatgccga
    18421 actgccagtt tatttcatca tgccctacaa ttctgtcttt cctaaaacaa gagcgacagg
    18481 atatactatg gagtactcaa ccttggatga atattttgta agcaaactat ggacaacgga
    18541 tcagaaactt tatgtctgga ctgtcaatga ttcagaagct atcagcaaat ctcttcactt
    18601 aggagtagat ggagtgatta cagatgattt ggaaaaagtt cagcgggaaa tagaagtagc
    18661 ccaagaagac ccagaatata cggatttgct cttgaaaaaa gcattggaat tctttgaatt
    18721 ttagtaataa aaaagaggaa cggttgttcc tcccatgata ggtaagtatt cttgtgaatg
    18781 cttgcttttt tgtggttgca atccgtttac gagaattgtt tgtattgttc ttgtttcaat
    18841 ttactttata gtgctttgaa gttggaatgc tacactataa cttctaaaat atagtgaaat
    18901 gaaccaaaaa tagtacacaa tgtggtataa tcttcttatg gcatattcaa tagattttcg
    18961 taaaaaagtt ctctcttatt gtgagcgaat aggtagtata acagaagcat cacacgtttt
    19021 ccaaatctca cgtaatacca tttatggctg gttaaagcta aaagagaaaa caggagagtt
    19081 aaac
//
LOCUS       DAVGJL010000022        18777 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.022, whole genome shotgun sequence.
ACCESSION   DAVGJL010000022 DAVGJL010000000
VERSION     DAVGJL010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18777
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.022"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            222..1667
                     /locus_tag="VVE42_000711"
     CDS             222..1667
                     /locus_tag="VVE42_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEU3072935.1"
                     /translation="MSRRFKKSRSQKVKRSVNIVLLTIYLLLVYFLLFLIFKYNILAF
                     RYLNLVVTALVLLVALVGLLLIIYKKAEKFTIFLLVFSILVSSVSLFAVQQFVGLTNR
                     LNATSNYSEYSISVAVLADSEIENVTQLMSVTAPTGTDNENIQKLLADIKSSQNTDLT
                     VNQSSSYLAAYKSLIAGETKAIVLNSVFENIIESEHPDYASKIKKIYTKGFTKKVEAP
                     KTSKDQSFNIYVSGIDTYGPISSVSRSDVNILMTVNRDTKKILLTTTPRDAYVPIADG
                     GNNQKDKLTHAGIYGVDSSIHTLENLYGVDINYYVRLNFTSFLKLIDLLGGVDVYNDQ
                     DFTSLHGKFHFPVGNVHLDSEQALGFVRERYSLADGDHDRGRNQQKVIAAILQKLTSS
                     EALKNYSRIIDSLQDSIQTNMPLETMINLVNAQLESGGTYKVNSQDLKGMGRTDLPSY
                     AMPDSNLYMMEINDSSLASVKTAIQDMLEGR"
     gene            1669..2400
                     /gene="cps4B"
                     /locus_tag="VVE42_000712"
     CDS             1669..2400
                     /gene="cps4B"
                     /locus_tag="VVE42_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4B"
                     /protein_id="HEU3072936.1"
                     /translation="MIDIHSHIVFDVDDGPKSREESKALLAESYRQGVRTIVSTSHRR
                     KGMFETPEEKIAENFLQVREIAKEVASDLVIAYGAEIYYTSDVLDKLEKNRIPTLNNS
                     RYALIEFSMNTPYRDIHSALSKILMLGITPVIAHIERYDALENNEKRVRELINMGCYT
                     QVNSSHVLKSKLFGERYKFMKKRAQYFLERDLVHIIASDMHNVDSRPPHMAEAYDLVS
                     QKYGEAKAQELFIDNPRKIVMDQLI"
     gene            2409..3101
                     /gene="cpsC"
                     /locus_tag="VVE42_000713"
     CDS             2409..3101
                     /gene="cpsC"
                     /locus_tag="VVE42_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CpsC"
                     /protein_id="HEU3072937.1"
                     /translation="MKEQNTIEIDVFQLLKTLWKHKLIILLVALVTGAGAFAYSTFIV
                     KPEYTSTTRIYVVNRNQGDKPGLTNQDLQAGTYLVKDYREIILSQDVLEKVATNLKLD
                     IPAKTLTSKVQVTVPADTRIVSISVKDKQPEEASRIANSLREVAAEKIIAVTRVSDVT
                     TLEEARPAMTPSSPNVRRNTLVGFLGGAAVTVITVLLIELLDTRVKRPEDVEDVLQMP
                     LLGVVPDFDKMK"
     gene            3111..3800
                     /locus_tag="VVE42_000714"
     CDS             3111..3800
                     /locus_tag="VVE42_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HEU3072938.1"
                     /translation="MPTLEISQAKLELVKKAEEYYNALCTNLQLSGDDLKVFSITSVK
                     AGEGKTTTSTNIAWAFARAGYKTLLIDADMRNSVMSGVFKSRERITGLTEFLSGTTDL
                     SQGLCDTNIENLFVIQAGSVSPNPTALLQSKNFSTMLETLRKYFDYIVVDTPPVGVVI
                     DAAIIMQKCDASILVTKAGETKRRDLQKAKEQLEQTGKSCLGVVLNKLDTSVEKYGSY
                     GSYGNYRKQKK"
     gene            3815..5176
                     /locus_tag="VVE42_000715"
     CDS             3815..5176
                     /locus_tag="VVE42_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEU3072939.1"
                     /translation="MSEKLAKPSVAIVQSFLVILLTYLLSAVRETEIVSTTAIVLYIL
                     HYFVFYISDYGRNFFKRGYLIELVQTLKYILFFALAISISNFFLEDRFSISRRGMIYF
                     LLLHVFLVYVLNPFIKWYWKRAYPNFKGSKKILLLTATSRVEKVLDRLIESNDVVGEL
                     VAVSVLDKPDFQHDDLKVVAEGEIVNFATHEVVDEVFINLPSEKYNIGELVSQFETMG
                     IDVTVNLNAFDWARNKQICEMAGLNVVTFSTTFYKTSHVIAKRIIDIVGSLVGLILCG
                     LVSIVLVPLIRKDGGSAFFAQTRIGKNGRHFTFYKFRSMCVDAEEKKRELMEQNTMQG
                     GMFKVDDDPRITKIGHFIRKTSLDELPQFYNVLKGDMSLVGTRPPTVDEYEHYTPEQK
                     RRLSFKPGITGLWQVSGRSEIKNFDEVVKLDVAYIDGWTIWKDIEILLKTVKVVLMKD
                     GAK"
     gene            5182..5925
                     /locus_tag="VVE42_000716"
     CDS             5182..5925
                     /locus_tag="VVE42_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEU3072940.1"
                     /translation="MNERIQILGITIDPLTMKETVDAVEQYVLKKHPLHLMGVNADKI
                     NQCYEDEKIKKIVNESGIINADGASVVLASKFLGTPVPERVAGIDLMKHLLELSNEKG
                     YSVYFFGAKEDVLQDMLKIFEERYPALNVVGYRNGYFSPEDEKHIQEDIKEKKPDFVF
                     VGITSPKKEYIIQSFMDNGINAVFMGVGGSFDVLSGHIKRAPLWMQNAHLEWLFRVAN
                     EPKRLFKRYFVGNVSFIRKVLRAKRGYKL"
     gene            5922..6746
                     /locus_tag="VVE42_000717"
     CDS             5922..6746
                     /locus_tag="VVE42_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HEU3072941.1"
                     /translation="MNQTEIVKLIQKVELDAIKEFQKICKENNIDFFLRGGSVLGAVK
                     YDGFIPWDDDMDIAVPREGYDKLPGIFKDRIIAGKYQVLAYQYCDTLHCYFPRLFLLE
                     DERKRLGLPRNTNLGLHLIDIIPLDGAPNHSFLRKLYFGKVYWYRFLASLGTTYVGDH
                     VDMHSAKQKLIIGFFKKLGFAKLFPQNYVYRRLDNLYKKYDWKKQKYAGTINASLFAK
                     EVMPVEIWGEGVEKPFEDAFFKVPTEYDRYLKRLYGENYLHEEPSDDEKKSHLGGQ"
     gene            6748..7626
                     /locus_tag="VVE42_000718"
     CDS             6748..7626
                     /locus_tag="VVE42_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU3072942.1"
                     /translation="MFCYIILHYKVLEETISCVKSIKEGNSNTKQIVIIDNFSNNGTG
                     EKLQELYESDSEIDVLINHENAGFARGNNVAYQFAKEKYKPDFMVIMNNDIEIETEEF
                     EKIVTDIYRKEKFHLLGPDIFSTTYQLHQNPKRLTHYTYEEVKALNEKFKKGSQVSLA
                     LKIKCWLKSSKVLRTAIYQNRRKKKSVDYRKQVENPILHGSFIVYSRDFIEKEEYAFN
                     PNTFFYYETEILDYEAELKGYKRIYTPKIKVLHHQNVATNQVYTNLVEKTLFSNKCNF
                     ESTSYFLKLMKENEGV"
     gene            7627..8961
                     /locus_tag="VVE42_000719"
     CDS             7627..8961
                     /locus_tag="VVE42_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide repeat unit polymerase"
                     /protein_id="HEU3072943.1"
                     /translation="MTYLFLLCLTLFLLTFFYFFAFNQDLIAPPVVMSVMFLISSVFA
                     LINVQNWNIEYSGLAYLLIISGIIVFSMPLLALNSPSLNTKIKVTDRLIDIQFWKIAL
                     TIIVDLVILYLYRREIHNLALSHGYTGSNFQWFFRNATSYEGELTVRTSIRVLIRIID
                     VSAYIFGYTFINNFFIYSHKRSKDLLLLVPFLIFISKTLLSGGRLDIIKILIAYVVMA
                     YIQQKRKVGWDKVISHKYMRLGFVGLIAGIPTFYYSLFLSGRSTTRTVFESISTYLGG
                     SIQHFNQYIQNPIGVAEVFGDESFVAIMNILGNLGFVNYNSTVHLEFRQLGITMGNVY
                     TFFRRPWHDFGLVGMYIFSFVVGVFFAIFYLKLRKSRAGFKLDIHTIIYSYFFYWIFL
                     SSIEQYSFTTISLFTLVFIVLVYLMAFFYWNLDFHRGKLVIKLSDTSIKSEN"
     gene            8989..10413
                     /locus_tag="VVE42_000720"
     CDS             8989..10413
                     /locus_tag="VVE42_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3072944.1"
                     /translation="MNSKIKNILTNFSYVISSNLLTVLTSSLVVLIFPKLMGVTEYSY
                     WQLYIFYLTYIGFFHLGWIDGIYLKYGGLEYNNLDRKQFYSQMILFSSFLMIISLLLF
                     GLNVLAVTDSNARYIYNMTIISMIITNLRTLYVYVLQMTNRLKDSSIILLSDRVLYVL
                     LLFLFIVFGWHEYKVMILADILGRSFSLILSFWICKDIVFQPLSKFIFNIKESFDNIR
                     VGINLMLSNIASSLIIGIVRLGIQWNWNIETFGKVSLTLSVSNLLMTFINAIGLVVFP
                     LLRRTKAENLPKIYSNLRNVLMLIMFAILLIYYPLKIVLDLWLPAYQDALIFMTLIFP
                     MSVYEGKMALVINTYLKALRMERDILRVNALVMLISMGVTLVTTYLLNSLELTVVSIV
                     VLLALRSIIAELILSKKLDVSVKKDIVLEFLLTLVFISSSWYLPIGLAVIVYTIAYGL
                     YLYLKHEDIKTYLAYFKASKKTSN"
     gene            10478..11566
                     /gene="wecB"
                     /locus_tag="VVE42_000721"
     CDS             10478..11566
                     /gene="wecB"
                     /locus_tag="VVE42_000721"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEU3072945.1"
                     /translation="MKKIMLVFGTRPEAIKMCPLVNELKKHEDMETIVCVTGQHKEMV
                     SPVLDLFGVVPDYDLEIMKANQTLFSITTSILEKIKPVLEKEQPDIVLVHGDTTTTYA
                     AALAAFYLGIKVGHVEAGLRTYNLQSPFPEEFNRQSTSIIATYHFAPTELAKENLLKE
                     GRENVYVTGNTVIDALTTTVQEDYTHTHLDLNANNRLILLTAHRRENLGEPMRHMFRA
                     VKRVLNEYEDVKVIYPIHKNPLVRETAAEIFGDTERIQIIEPLDVLDFHNFMNQSYMI
                     LTDSGGVQEEAPSLGKPVLVMRDTTERPEGVAAGTLKLVGTDEETIYQNFKLLLDDSG
                     EYKKMSQASNPYGNGDASQQIVQILRGI"
     gene            11729..12598
                     /gene="rfbA"
                     /locus_tag="VVE42_000722"
     CDS             11729..12598
                     /gene="rfbA"
                     /locus_tag="VVE42_000722"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002276675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEU3072946.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPVYDKPMIYYPLSTLMLA
                     GIKDILIISTPQDLPRFKDLLLDGSEFGIKLSYAEQPSPDGLAQAFLIGEEFIGDDSV
                     ALILGDNIYHGPGLSTMLQKAAKKEKGATVFGYQVKDPERFGVVEFDTDMNAISIEEK
                     PEYPRSNYAVTGLYFYDNDVVEIAKQIKPSARGELEITDVNKAYLNRGDLSVELMGRG
                     FAWLDTGTHESLLEASQYIETVQRMQNVQVANLEEISYRMGYISREDVLELAQPLKKN
                     EYGQYLLRLIGEA"
     gene            12599..13192
                     /locus_tag="VVE42_000723"
     CDS             12599..13192
                     /locus_tag="VVE42_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEU3072947.1"
                     /translation="MSDNFFGKTLVVRKIDAIPGLLEFDIPVHGDNRGWFKENFQKEK
                     MLPLGFPESFFAAGKLQNNVSFSRKNVLRGLHAEPWDKYISVADDGKVLGSWVDLREG
                     ETFGNVYQTEIDASKGIFVPRGVANGFQVLSDTVSYSYLVNDYWALELKPKYAFVNYA
                     DPALGIEWENLAEAEVSEADKHHPLLRDVKPLTKDEL"
     gene            13204..14253
                     /gene="rfbB"
                     /locus_tag="VVE42_000724"
     CDS             13204..14253
                     /gene="rfbB"
                     /locus_tag="VVE42_000724"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEU3072948.1"
                     /translation="MTEYKKIIVTGGAGFIGSNFVHYVYNNFTDVHVTVLDKLTYAGN
                     RANIEEILGDRVELVVGDIADAALVDKLAAEADAIVHYAAESHNDNSLNDPSPFIHTN
                     FIGTYTLLEAARKYDLRFHHVSTDEVYGDLPLREDLPGHGEGPGEKFTAETKYNPSSP
                     YSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILSGIKPKLY
                     GEGKNVRDWIHTNDHSSGVWTILTKGQIGETYLIGADGEKNNKEVLELILKEMGQPAD
                     AYDHVTDRAGHDLRYAIDASKLRDELGWKPEFTNFEAGLKATIKWYTDNQDWWKAEKE
                     AVEANYAKTQEIITV"
     gene            14319..15170
                     /gene="rfbD"
                     /locus_tag="VVE42_000725"
     CDS             14319..15170
                     /gene="rfbD"
                     /locus_tag="VVE42_000725"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEU3072949.1"
                     /translation="MILITGANGQLGTELRYLLDERNEEYVAVDVAEMDITNEEMVEK
                     VFEEVKPTLVYHCAAYTAVDAAEDEGKELDFAINVTGTENVAKASEKHGATLVYISTD
                     YVFDGKKPVGQEWEVDDRPDPQTEYGRTKRMGEELVEKHVSNFYIIRTAWVFGNYGKN
                     FVFTMQNLAKTHKTLTVVNDQYGRPTWTRTLAEFMTYLAENRKEFGYYHLSNDATEDT
                     TWYDFAVEILKDTDVEVKPVDSSQFPAKAKRPLNSTMSLAKAKATGFVIPTWQDALQE
                     FYKQEVR"
     gene            15233..15567
                     /locus_tag="VVE42_000726"
                     /pseudo
     CDS             15233..15567
                     /locus_tag="VVE42_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272501.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
     gene            15834..16419
                     /locus_tag="VVE42_000727"
                     /pseudo
     CDS             15834..16419
                     /locus_tag="VVE42_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001835598.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            16656..18638
                     /locus_tag="VVE42_000728"
     CDS             16656..18638
                     /locus_tag="VVE42_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU3072950.1"
                     /translation="MKSSKLFALAGVTLLVATTLAACSGSGSSTKGEKTFSYIYETDP
                     DNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYAIRKDA
                     KWYTSEGEEYAAVKAQDFVTGLKYAADKKSDALYLVQESIKGLDAYVKGEIKDFSQVG
                     IKALDEQTVQYTLNKPESFWNSKTTMGVLAPVNEEFLNSKGDDFAKATDPSSLLYNGP
                     YLLKSIVTKSSVEFAKNPNYWDKDNVHIDKVKLSFWDGQDTSKPAENFKDGSLTAARL
                     YPTSASFAELEKSMKDNIVYTQQDSITYLVGTNIDRQSYKYTSKTSDEQKASTKKALL
                     NKDFRQAIAFGFDRTAYASQLNGQTGASKILRNIFVPPTFVQADGKNFGDMVKEKLVT
                     YGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVTFPIHLDMPVDQTATTKVQ
                     RVQSMKQSLEATLGADNVIIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFA
                     DPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDEATDVAKRY
                     DKYAAAQAWLTDSALIIPTTSRTGRPILSKMVPFTIPFALSGNKGTSEPVLYKYLELQ
                     DKAVTVDEYQKAQEKWMKEKEESNKKAQEDLAKHVK"
BASE COUNT         5941 a         3118 c         3962 g         5756 t
ORIGIN      
        1 ttagattgaa actagaatag tacacctctg cttctaaaac attgttagaa atcgatttga
       61 ctgtcctgat cgatttgtca tgttcttatt tcattttact atatttttgt ttcgcgggaa
      121 gtctactaag atacttaaag atgcagatag taaaaaaaaa tgtagacatt accgtaaaaa
      181 agtgatataa tcgtatgatg ttcaaggtat aggtgttaat catgagtaga cgttttaaaa
      241 aatcacgttc acagaaagtg aagcgaagtg ttaatatcgt tttgctgact atttatttat
      301 tgttagttta ttttttattg ttcttaatct ttaagtacaa tatccttgct tttagatatc
      361 ttaacctagt ggtaactgcg ttagtcctac tagttgcctt ggtagggcta ctcttgatta
      421 tctataaaaa agctgaaaag tttactattt ttctgttggt gttctctatc cttgtcagct
      481 ctgtgtcgct ctttgcagta cagcagtttg tgggactgac caatcgttta aatgcgactt
      541 ctaattactc agaatattcg atcagtgttg ctgttttagc agatagtgag attgagaatg
      601 ttacgcaact gatgagtgtg acagcaccga ctgggactga taatgaaaac attcaaaaac
      661 tactagctga tatcaaatca agtcagaata ccgatttgac ggtcaatcag agttcgtctt
      721 acttggcagc ttataagagt ttgattgcag gagagactaa ggccattgtc ttaaatagtg
      781 tctttgaaaa tatcatcgaa tcggagcatc cagattacgc atcgaagata aaaaagattt
      841 ataccaaggg attcactaaa aaagtagaag ctcctaagac gtctaaggat cagtctttca
      901 atatctatgt tagtggaatt gatacttatg gtccgattag ttcggtgtcg cgttcagatg
      961 tcaatatcct gatgactgtc aatcgagata ccaagaaaat tctcttgacc acaacgccac
     1021 gtgatgctta tgtaccaatc gcagatggtg ggaataatca aaaagataaa ttgacccatg
     1081 cgggcatcta tggagttgat tcgtccatcc acactttaga aaatctctat ggagtggata
     1141 tcaattacta tgtgcgattg aacttcactt ctttcttgaa attgatcgat ttgttgggtg
     1201 gggtagatgt ttataatgac caagatttta catctctaca cgggaagttc catttcccag
     1261 ttggaaatgt ccacttagat tctgagcagg ctctgggatt tgttcgtgag cgctattccc
     1321 tagcggatgg tgatcatgac cgcgggcgca atcaacaaaa ggtgattgcg gccatccttc
     1381 aaaaattaac ttcgagcgaa gcgctgaaaa attatagtag gatcattgat agcttgcaag
     1441 attctatcca aacaaacatg ccacttgaga ccatgataaa cttggtcaat gctcagttag
     1501 aaagtggtgg aacgtacaaa gtgaattcgc aagacttgaa gggtatggga cggacggatc
     1561 ttccttccta tgcgatgcca gatagtaacc tctatatgat ggaaattaac gacagtagcc
     1621 ttgcatctgt caaaactgct attcaggata tgttggaggg cagatgaaat gattgatatt
     1681 cattcgcaca tcgtctttga tgtagatgat ggtcccaagt caagagagga aagcaaggct
     1741 ctcttggcag aatcctacag gcagggggtg cgaaccattg tctctacctc tcaccgtcgc
     1801 aagggcatgt ttgaaactcc ggaagagaag atagcagaaa actttcttca ggttcgggaa
     1861 atagctaagg aagtggcgag tgacttggtc attgcttacg gggctgaaat ttactacaca
     1921 tcagatgttt tggataagct ggaaaaaaat cggattccga ccctcaataa tagtcgttat
     1981 gccttgatag agtttagtat gaacacccct tatcgcgata ttcatagtgc cttgagtaaa
     2041 atattgatgt tgggaattac tccagtcatt gcccacattg agcgctatga tgcacttgaa
     2101 aataatgaaa aacgcgttcg agagctgatc aatatgggct gttacacgca agtaaatagt
     2161 tcacatgtcc tcaaatccaa actttttgga gaacgttata aattcatgaa aaaaagagct
     2221 cagtattttt tggagcgtga cttggttcat atcattgcaa gtgatatgca taatgtggac
     2281 agcagacctc cccatatggc agaagcatat gaccttgttt cccaaaaata cggagaagcg
     2341 aaggctcagg aactttttat agataatcct cgaaaaattg taatggatca actaatttag
     2401 gagaaatgat gaaagaacaa aacacgatag aaatcgatgt atttcaatta cttaaaacct
     2461 tgtggaaaca caagctaata attttattag tggcacttgt gacaggggcg ggagcttttg
     2521 catatagcac ttttattgtt aagccagaat atacaagcac cacgcgtatt tacgtagtca
     2581 accgtaatca aggagataag ccgggactga cgaatcagga cttgcaggca ggaacttatc
     2641 tggtaaaaga ctaccgcgaa attatccttt cgcaagatgt attggaaaag gtagcgacaa
     2701 atttgaaatt ggatattcca gcaaaaacgt taactagcaa agtgcaagtg actgtaccag
     2761 ccgacactcg tatcgtctca atctctgtca aggataaaca accagaggaa gccagtcgca
     2821 ttgctaattc tctacgagaa gttgctgcag aaaagatcat cgctgtaacg cgagtatctg
     2881 atgtaacgac gcttgaagaa gcgcgaccag ctatgactcc ctcttctcca aatgttcgac
     2941 gcaacacctt agttggtttt cttggaggag ccgccgtaac agtaattact gttcttttga
     3001 ttgagttgct cgatacccgt gtaaaacgtc ctgaagatgt tgaagatgta ctgcaaatgc
     3061 cacttctagg agtcgttcca gattttgata aaatgaaata ggaggaagtt atgccaacgt
     3121 tagaaatctc acaggcgaaa ttagaacttg tgaaaaaggc agaggaatat tataatgctt
     3181 tgtgcacgaa cctacagtta agtggagatg atttgaaagt attttctatc acttctgtga
     3241 aagcaggaga aggaaaaaca acgacttcca ccaatatcgc ttgggctttt gcgcgtgcag
     3301 gttacaaaac gcttctgatt gatgcagaca tgcgtaactc cgttatgtca ggtgtcttta
     3361 aatcaaggga aaggattact ggactaacag aatttctatc agggacaaca gacctgtcac
     3421 aggggctttg cgataccaat attgagaatc tctttgtgat tcaggctggc tctgtgtcac
     3481 caaatccgac agcccttctt caaagtaaga atttcagtac aatgcttgaa accttgcgta
     3541 aatattttga ctatatcgtc gtagacactc cccctgtcgg tgtcgtgatt gatgcggcta
     3601 tcattatgca gaaatgtgat gcttctattt tagtgacgaa ggcaggtgaa acaaaacgac
     3661 gggatcttca aaaagcgaaa gaacagttgg aacaaactgg gaagtcgtgt ttaggagttg
     3721 tgttgaataa actcgatact tcagtagaga aatacggttc ttatgggagt tatgggaatt
     3781 acaggaaaca gaaaaaatag gttgagggat agaaatgagt gaaaaactag caaagccttc
     3841 agtagccata gtccagagtt ttcttgttat tttattgact tatctactta gcgctgtgag
     3901 agaaacagag attgtttcaa caacagctat tgtactttat atcctccact attttgtctt
     3961 ttatatcagt gattatggac ggaatttctt taaaagggga tatttgattg aacttgtcca
     4021 gacattgaaa tatatcctat tctttgcgct agcgattagt atttctaatt ttttcttaga
     4081 agatcgattt agtatttcca gaagaggcat gatttacttc ctcttattac atgttttctt
     4141 agtctatgtg ctaaacccat ttatcaagtg gtattggaag cgggcttatc ccaactttaa
     4201 aggaagtaag aagattctcc tacttacagc aacttctcgt gttgaaaagg tactggatag
     4261 attaatagaa tcaaatgatg ttgttgggga gttggtagcc gtcagtgttt tagataaacc
     4321 agattttcag catgatgatt taaaggttgt agcagagggg gagatagtaa attttgcgac
     4381 tcatgaggtg gtcgatgaag tctttatcaa tcttccaagt gaaaaataca atattggaga
     4441 gcttgtctct cagtttgaaa cgatgggaat tgatgtaaca gtcaatctaa atgctttcga
     4501 ttgggcacgt aacaagcaaa tttgtgagat ggcaggacta aatgttgtga ctttttctac
     4561 aacattttat aagactagcc atgtgattgc taagcggatt attgatattg tcggttccct
     4621 ggtaggtttg atattatgtg gtctagtcag tattgtgctg gttcctttga ttcgaaagga
     4681 tgggggttct gctttttttg ctcagacgcg tataggaaaa aatggccgcc attttacttt
     4741 ttataagttt cgctctatgt gtgtggatgc tgaggagaaa aaaagagaac tcatggaaca
     4801 aaataccatg cagggtggaa tgtttaaggt ggacgatgat cctcgtatca cgaaaattgg
     4861 tcattttata cggaagacga gcttggacga gctaccacag ttttataatg ttctaaaggg
     4921 agatatgagt ttggtaggta cacgaccacc aacagtggac gagtatgagc actatacccc
     4981 agaacaaaaa cgtcgcctaa gttttaaacc tggcataaca ggtttatggc aggtcagcgg
     5041 acgaagtgaa atcaaaaatt tcgatgaagt tgtcaaatta gatgtggcct atattgatgg
     5101 ttggacaatc tggaaagata ttgaaatttt attgaagaca gttaaggttg tattgatgaa
     5161 ggatggagcg aaatagttca tatgaatgag agaattcaaa tattaggaat tactatagat
     5221 ccattaacga tgaaagaaac agtggatgct gttgaacagt atgttttaaa gaagcaccct
     5281 ttgcacttga tgggagttaa cgcggataaa attaatcagt gctatgaaga tgagaaaatc
     5341 aaaaaaatcg ttaatgaatc aggaataatt aatgccgacg gtgcttcagt agttttagcg
     5401 agtaagtttt taggaacacc agttcctgag cgagttgctg gaatagactt gatgaaacat
     5461 ttactagagt tgtctaatga aaaaggatac tcggtctact tttttggagc aaaagaagac
     5521 gttttacaag atatgttaaa aatttttgaa gaaagatatc cagctctaaa tgttgttggc
     5581 tatagaaatg gatatttttc gcctgaagat gaaaaacata ttcaggaaga tattaaagag
     5641 aaaaaaccag attttgtgtt tgtaggaatt acttctccta agaaagaata tattatccaa
     5701 tcattcatgg ataatggaat taatgctgtg tttatggggg ttggtggaag ttttgatgta
     5761 ctatcagggc atatcaaacg agctccatta tggatgcaaa atgctcacct agagtggttg
     5821 tttcgtgtgg caaatgagcc taaacgtctc tttaagcgtt attttgtagg gaatgtttca
     5881 tttataagaa aagttttaag agcgaaaagg ggatataaac tttgaaccaa acagaaatag
     5941 ttaaattgat tcagaaagtt gaattggatg ctataaaaga gtttcaaaaa atttgtaaag
     6001 agaacaatat agatttcttc cttcgtgggg ggagtgtact tggggcagtc aaatatgatg
     6061 gctttattcc atgggatgat gacatggata ttgctgtgcc tcgtgaaggc tacgataaac
     6121 ttccaggtat ctttaaggat agaattatcg caggcaaata tcaggttctt gcttatcaat
     6181 attgcgatac tttacattgc tactttcctc gcttattcct tttagaagat gaaagaaaac
     6241 gtttgggatt gccacgaaat accaatttag gtttgcattt gattgatatc attcctttgg
     6301 atggtgcccc aaatcattca tttttaagaa agctttactt cggtaaagta tactggtatc
     6361 gttttttagc aagtttagga actacctatg ttggagacca tgtggatatg cattccgcta
     6421 agcaaaaact aatcattggt ttctttaaaa agctaggatt tgcaaaacta ttccctcaaa
     6481 attatgtgta cagacgcttg gataatctct ataaaaagta tgattggaaa aagcagaagt
     6541 atgctgggac aattaatgca tctctgtttg ccaaagaagt catgccagta gagatttggg
     6601 gagaaggagt agagaagcct tttgaggatg ccttctttaa agttcccacg gagtatgacc
     6661 gctatctgaa aagactttac ggagaaaact atcttcacga agagcctagt gatgatgaaa
     6721 agaaatcgca tttaggagga caataatttg ttttgttata ttattttgca ttacaaagtc
     6781 ttagaggaaa ctatttcttg tgttaaatca ataaaagaag gcaattccaa tacaaagcag
     6841 atcgttatta ttgataattt ttctaataac ggtacgggtg aaaaactaca agagctgtat
     6901 gagtcagatt cagagattga tgtcttgatt aaccatgaaa atgctggttt cgctcgaggt
     6961 aataatgtag catatcagtt tgctaaggaa aagtacaaac ctgattttat ggttatcatg
     7021 aataatgata ttgagataga aacagaagag tttgaaaaaa tcgtgacaga tatctatcgg
     7081 aaggaaaaat tccatttgtt aggaccagat atcttttcga cgacgtatca gcttcaccaa
     7141 aacccaaaac ggttgacgca ttatacttat gaagaggtta aggctctcaa tgaaaaattt
     7201 aagaaaggga gccaagttag tctagcatta aaaattaaat gttggttgaa gtctagtaaa
     7261 gttcttcgga cagcaatcta tcaaaatagg cgtaaaaaga aatcagtaga ctatagaaaa
     7321 caggtagaaa acccaattct tcatggttcg tttattgtat attctagaga ttttattgag
     7381 aaagaggagt atgcttttaa tcccaatacc ttcttctatt atgaaacaga gatattagat
     7441 tatgaagctg agttaaaagg atataagaga atttatacac cgaagattaa ggtcttgcac
     7501 catcaaaatg ttgcaaccaa tcaagtctat acaaacttag tagaaaaaac cttgttttca
     7561 aacaaatgca actttgaatc cactagttat tttttgaagt tgatgaaaga aaacgagggt
     7621 gtgtaaatga cttatttatt tttactctgc ctgaccttat tcttattaac tttcttctat
     7681 ttttttgctt tcaatcaaga tttgatagct ccgccagttg tcatgtctgt tatgtttctc
     7741 attagttctg tatttgccct tattaatgtg caaaactgga atattgagta tagtggttta
     7801 gcttatcttt tgattattag tggcattatt gttttttcaa tgcctctctt ggctcttaat
     7861 tcgcctagtc taaataccaa gattaaagtg acggatcggc tcattgatat ccaattctgg
     7921 aaaatagctc ttactattat agttgacctc gttattctat atctttatag gagagagatt
     7981 cataatcttg cacttagcca tggttatacg ggttcaaatt ttcagtggtt ctttagaaat
     8041 gctaccagtt atgaaggtga gctaacagtg cgaacttcga ttcgggtcct cattcgtatc
     8101 attgacgtat ctgcttatat ttttggatat acttttatta ataatttctt catttatagt
     8161 cataaacgct ctaaagattt actgctctta gttccattct tgatttttat ttctaaaacc
     8221 ttattatctg ggggtagatt ggatattata aaaattttaa ttgcgtatgt tgtaatggcc
     8281 tatattcagc aaaaacgaaa agttggctgg gataaggtca tctcccataa atatatgaga
     8341 cttggttttg taggcttgat agctgggatt cctacctttt actattcttt attcttatct
     8401 ggacgttcta cgactagaac tgtatttgaa agtatttcaa cctatttagg aggttcgatt
     8461 cagcatttta atcagtatat tcaaaatcct attggagtag ctgaggtttt tggcgatgag
     8521 tcatttgtag ctattatgaa tattttgggt aatcttggct ttgtcaatta caatagtacc
     8581 gttcacttag aatttcggca gttagggatt actatgggta acgtttatac tttctttaga
     8641 agaccctggc atgactttgg gttagtcggt atgtatatct tttcctttgt tgtcggtgtg
     8701 ttttttgcta ttttttattt gaaattaaga aagagccgtg ctggttttaa attggatatt
     8761 catacaatta tttactctta tttcttttat tggatttttt tatcgtccat tgagcaatac
     8821 tcgtttacaa caatcagttt gtttacactt gtatttatag tcttggttta cctaatggct
     8881 ttcttctatt ggaatttgga tttccacaga ggaaaactag taattaaact ctctgacaca
     8941 agtattaaat cggaaaactg aaaacaataa aaatagagag gatagttgat gaatagcaaa
     9001 attaaaaata tactaactaa tttctcttat gtcatttctt caaatctttt gacagtcttg
     9061 acctcttcct tggttgtctt aattttcccc aaattaatgg gggtaacaga gtacagttac
     9121 tggcaacttt atatttttta tctgacctat atcggttttt tccacttggg ttggattgat
     9181 gggatttatc ttaaatatgg tggcttagaa tataataatt tagatagaaa acagttttat
     9241 tctcagatga ttctattttc tagcttctta atgattatct ccttgctatt gtttggttta
     9301 aatgtattag ctgtaacaga ttcgaatgca cgatatattt ataatatgac tattataagt
     9361 atgataatca caaatttaag aacgctctac gtgtatgttt tgcagatgac aaaccgcttg
     9421 aaggatagtt ccatcattct actcagtgat cgcgttttat atgtgctcct tttattcctg
     9481 tttattgtat ttggttggca tgaatacaag gttatgattt tggcagatat tctaggtcga
     9541 tcattttctc tcatactttc cttctggatt tgtaaagata ttgtgtttca gcctttgtca
     9601 aaattcatct ttaatataaa agagtctttt gataatatcc gagttggtat caatttaatg
     9661 ttatccaaca tcgcaagtag tttgattatc ggtattgttc gtctaggaat ccaatggaac
     9721 tggaatatcg aaacattcgg gaaagtatct ctgaccttga gtgtttccaa tctattgatg
     9781 actttcatta atgccattgg actagtcgtc tttccgctat taagacgaac aaaagcagaa
     9841 aacttaccta agatttattc taatttaaga aacgttttga tgcttatcat gttcgcgatt
     9901 ttgctcattt actatccttt aaaaattgta ttagacctct ggttgccagc ctatcaagat
     9961 gccttgattt tcatgaccct tattttccct atgtcagtct atgaagggaa aatggcattg
    10021 gtcattaata cttacttaaa ggcattaaga atggaaagag atatccttag agtcaatgcc
    10081 ttagtaatgt tgatcagtat gggagtgacc ctggttacaa catacctgtt aaatagtttg
    10141 gagctgactg ttgtatcgat agttgttttg ctagctttga gaagtatcat agccgaatta
    10201 attctgtcta aaaaactgga tgtttcggtt aagaaggata ttgtattaga atttcttttg
    10261 acgcttgtct ttatttcttc aagttggtat ttgccgattg ggctggcagt aattgtctac
    10321 acaatagcct acggtttata tctctatttg aaacacgaag atatcaaaac ctatttagct
    10381 tactttaaag ctagtaaaaa aacatcaaat taaaaaatta tataatgatt aagtggtaga
    10441 ttctatttct accgttttag atattcggga ggtaatgatg aaaaaaatta tgttagtatt
    10501 cggtacacgt ccggaagcca tcaaaatgtg tccattagtc aatgagttga aaaaacacga
    10561 agatatggaa acaattgtgt gtgttactgg acaacacaaa gagatggtta gtcctgtttt
    10621 agatttattt ggtgttgtac cagattatga tttagaaatt atgaaggcta accaaacctt
    10681 gttctctatc acaactagta tcttggaaaa gataaaacca gttttagaga aggaacaacc
    10741 agatattgtc ctagttcacg gtgacactac gacaacttat gcagcagcct tggcagcatt
    10801 ctatttggga attaaagtag gacatgttga agctggtttg cgaacgtaca atttacaaag
    10861 tccatttcct gaagaattta acaggcaatc gacatcaatc attgcaactt accattttgc
    10921 tccaactgag ttggctaaag aaaatctctt aaaagaaggt agagagaatg tttatgtgac
    10981 tggaaatact gtcattgatg ctcttacaac tactgttcaa gaggattata cacacactca
    11041 tttagattta aacgctaaca atcgtctcat cttattgact gctcatagac gcgaaaatct
    11101 cggcgaaccg atgagacata tgtttagagc agttaaacga gtattgaatg aatacgaaga
    11161 tgttaaagtc atttatccaa ttcataagaa tcctttggta cgtgaaacag ctgcagagat
    11221 ctttggagat acagaacgga ttcaaattat tgaacctttg gatgttcttg atttccacaa
    11281 cttcatgaac caaagttata tgattttaac agattctggc ggagttcagg aagaagcacc
    11341 ttctttagga aaacctgtat tggttatgcg cgatactact gaaagacctg aaggagtagc
    11401 ggcaggaacc ttgaaattag taggaactga cgaagaaaca atttatcaaa acttcaaact
    11461 acttctagat gattctggag aatacaaaaa aatgagtcag gctagcaatc catacggtaa
    11521 tggtgatgct agtcaacaga ttgttcagat tttgcgtggg atttaagaag attctctaaa
    11581 agagtctaag aggaatccac tccacaaaca taaaactctt atgctatcat ttatgattga
    11641 gatagactca taacttaata tttttatcct aagaaaattc gttcatattc aatatgaatg
    11701 aaaaacaacg aagaaaggtt atttcattat gaaaggtatt attcttgcag gcggctcagg
    11761 tacccgcctg tacccactta ctcgggctgc gtcaaaacag ctgatgccgg tttatgataa
    11821 acctatgatt tattatccgt tgtcgacatt aatgttggct ggaattaaag atattttgat
    11881 tatctcaact cctcaagatt tgccccgttt taaggacttg ctcttggatg gttccgaatt
    11941 tgggatcaag ctttcctatg cggaacaacc tagtcccgat ggacttgctc aggcttttct
    12001 tatcggtgaa gaatttatcg gtgacgatag cgttgccttg attttaggcg acaatatcta
    12061 ccatggtcct ggtctgagca caatgcttca aaaagcagcc aagaaagaga aaggtgcgac
    12121 tgtttttggc taccaagtga aggatccaga gcgttttggt gtggttgagt ttgatacaga
    12181 catgaatgct atttctatag aagaaaaacc ggagtatcct cgctccaact atgcagtgac
    12241 aggactgtat ttctatgata atgatgttgt ggagattgct aaacagatca aacctagtgc
    12301 tcgtggcgag ttagaaatta cagacgttaa caaggcttac ctaaatcgtg gtgacctttc
    12361 tgttgagctg atggggcgtg gttttgcctg gttggatacg ggaacccatg aaagcttgct
    12421 agaagcttct cagtatattg aaacggttca gcgtatgcag aacgttcaag tggcaaatct
    12481 agaagaaatt tcctatcgta tgggctacat tagtcgtgaa gacgtgctgg aattggctca
    12541 gcctcttaag aaaaatgaat acggacaata tttgctccgt ttgattggag aagcatagat
    12601 gtcagataat ttttttggaa agacacttgt ggtacgcaag attgatgcta taccaggact
    12661 gctagagttt gatattcccg ttcatggaga caatcgtggt tggtttaagg aaaatttcca
    12721 gaaggaaaag atgctaccgc ttggttttcc tgaaagcttc tttgctgcag ggaaactgca
    12781 aaataacgtc agcttttctc gcaaaaatgt tcttcgagga ctccatgctg aaccttggga
    12841 caagtatatc tctgttgcag acgatgggaa ggttttagga tcttgggtag atttgcgtga
    12901 aggcgagacc tttggaaatg tttaccagac agagattgat gctagcaagg gaatttttgt
    12961 ccctcgtggc gtagccaacg gttttcaagt cctctcagat acggtttcct atagctatct
    13021 ggtcaatgac tactgggctc ttgaactcaa acccaaatat gcctttgtta actatgctga
    13081 cccagcattg ggaattgagt gggaaaacct agcggaagct gaggtttcag aggcagacaa
    13141 acaccatcct ctattaaggg atgtcaaacc acttacgaaa gacgagttgt aaaaggaaac
    13201 attatgactg aatacaaaaa aattatcgtg acaggtggag ctggttttat cggttctaac
    13261 tttgttcact atgtttacaa taactttaca gatgtccatg tgacagtgct ggacaagctg
    13321 acttatgcag gtaatcgtgc caatattgaa gaaattttag gcgaccgtgt tgagttagtc
    13381 gttggagata ttgctgatgc agccttggta gataagttag cagctgaagc ggatgccatt
    13441 gtacactatg cggcagaaag ccacaatgac aattcgctta atgacccctc gccatttatc
    13501 cataccaact tcatcggaac ttatacactc ttggaagcgg ctcgtaaata cgaccttcgt
    13561 ttccaccatg tgtcgactga cgaagtctat ggggatctgc cgctgcgtga agatttacca
    13621 ggtcatgggg aaggaccagg tgagaaattt acggctgaaa ccaagtacaa tccaagctcg
    13681 ccttactcat cgactaaggc ggcttcagac ttgattgtca aagcttgggt gcgctcattt
    13741 ggtgttaaag caacgatttc taactgttca aacaactatg gtccttacca gcacatcgag
    13801 aagttcattc cgcgccagat tactaatatc ttgagcggta tcaagccaaa actctacgga
    13861 gaaggtaaga atgtgcgtga ctggattcat actaatgacc attcatcagg tgtttggacg
    13921 attctgacca agggtcaaat cggtgaaacc tacctgattg gtgcggatgg tgagaagaac
    13981 aataaggaag tgttggaact catcctcaaa gaaatgggac agccagctga tgcttatgac
    14041 cacgtaacag atcgagctgg tcacgacctc cgctatgcca tcgatgcaag caagctccgt
    14101 gatgaactag gatggaagcc agagtttacc aactttgaag caggtctcaa agcgactatc
    14161 aagtggtaca cagataacca agactggtgg aaagcagaaa aagaagctgt tgaagccaat
    14221 tatgctaaga ctcaggagat tattacagta taaaaagcag gaaatcgctg ctttttattg
    14281 ctatattggg aagagttaca tattagaaag gtctagagat gattttaatt acaggggcaa
    14341 atggccaatt aggaacggaa cttcgctatt tattggatga acgtaatgaa gaatacgtgg
    14401 cagtagatgt ggctgagatg gacattacca atgaagaaat ggttgagaaa gtttttgaag
    14461 aggtgaaacc aactttagtc taccactgtg cagcctatac tgctgttgat gcagcagagg
    14521 atgaaggaaa agagttggac ttcgccatca atgtgacggg gacagaaaat gtcgccaaag
    14581 catctgaaaa gcatggtgca actctagttt atatttctac ggactatgtc tttgacggta
    14641 agaaaccagt tggacaagag tgggaagttg atgaccgacc agatccacag acagaatatg
    14701 gacgcactaa gcgtatgggg gaagagttag ttgagaagca tgtttctaat ttctatatta
    14761 tccgtactgc ctgggtattt ggaaattatg gcaaaaactt cgtttttacc atgcaaaatc
    14821 ttgcgaaaac tcataagact ttaacagttg taaatgacca gtacggtcgt ccgacttgga
    14881 ctcgtacctt ggctgagttc atgacctacc tagctgaaaa tcgtaaggaa tttggttatt
    14941 atcatttgtc aaatgatgcg acagaagaca caacatggta tgattttgca gttgaaattt
    15001 tgaaagatac agatgtcgaa gtcaagccag tagattccag tcaatttcca gccaaagcta
    15061 aacgtccgct aaactcaacg atgagcctgg ccaaagccaa agctactgga tttgttattc
    15121 caacttggca agatgcattg caagaatttt acaaacaaga agtgagataa gtagtagaat
    15181 gattttctag tctaataaaa gaggcagata atgaactcaa aaggagctta aggtgtacga
    15241 ttatcttgtt gttggtgctg gtctctttgg tgcagtcttt gcccatgaag tagccttaaa
    15301 aggaaaaaag taaaagtcat tgaaaaacgg aatcatatcg tgggtaatat ctatactcgt
    15361 gaagaggaag gaattcaagt tcatcaatat ggtgctcata tcttccatac ttctgataag
    15421 gagagctggg attatgtaaa tcagtttgca gggtttaacc gttacaccaa ttctcctgtt
    15481 gcaaactata agggtgagat ttataacctt ccttttaata tgaatacttt caataaactc
    15541 tggggagttg taacgccagc agaagcacaa gctaagattg aggaacaacg tgctatttta
    15601 aatggtaaaa ctcctgaaaa tttggaagaa caggcgattt ctcttgtagg tacagacatc
    15661 tacgaaaaat taatcaaaga ctatacagag aaacagtggg gcaaaccaac tactgaactt
    15721 ccatccttta ttattcgccg tttaccagta cacctgacct atgataacaa ctattttaac
    15781 gatacctatc aagggattcc aattggtgga tacactcaaa tagttgaaaa atgttggatt
    15841 atgaaaatat tgatgtagaa acaaatgttg atttctttgt gaacaaagag caatatctga
    15901 aagattttcc taagattgtc tttactggta tgattgatga attctttgac tataagttgg
    15961 gcgaactaga gtaccgtagt cttcgttttg aaaatgagac cttggatatg gaaaattacc
    16021 aaggaaatgc agttgtgaac tatacggatg cagaaacccc atatactcgc attattgaac
    16081 acaaacattt tgagtttggg agtcaagcaa agactatcat tactaataga acattctaaa
    16141 acatgggaaa aaggtgatga gccttattat ccagttaata atgatcgtaa taatcatttg
    16201 tataaatcat ataaaaaact tgctgatgag caagggaatg ttatctttgg tggccgctta
    16261 ggacactatc gttattacga tatgcaccaa gtaattggag cagctttgca gtgcgtgaga
    16321 aatgagttag attcatactc aatgaaaatc aaagagcaaa ctaggaagct agccacaggt
    16381 tgctcaaaat actgttttga ggttgcagat ggaagctgac gcggtttgaa gagattttcg
    16441 aagagtataa acaagtaaaa ctgactacca gttattattt agaaatagta ttaaaaattc
    16501 cttgactatg tgatatagtt gagggatttt taaatgatat tcatattttt tgcaaagatg
    16561 ttgtttgaaa aataattttc aaaaattctg aaaattctgt tgacatcttt ctgaaaagag
    16621 tctataatgg agagaaagtt ttaaaggaga aaatgatgaa aagttcaaaa ctatttgccc
    16681 ttgcgggcgt gacattattg gtggcgacta ctttagctgc atgctctgga tcaggttcaa
    16741 gcactaaagg tgagaagaca ttctcataca tttatgagac agaccctgat aacctcaact
    16801 atttgacaac tgctaaggct gcgacagcaa atattaccag taacgtggtt gatggtttgc
    16861 tagaaaatga tcgctacggg aactttgtgc cgtctatggc tgaggattgg tctgtatcca
    16921 aggatggatt gacttacact tatgctatcc gtaaggatgc aaaatggtat acttctgaag
    16981 gtgaagaata cgcggcagtc aaagctcaag actttgtaac aggactaaaa tatgctgctg
    17041 ataaaaaatc agatgctctt taccttgttc aagaatcaat caaagggttg gatgcctatg
    17101 taaaagggga aatcaaagat ttctcacaag taggaattaa ggctctggat gaacagacag
    17161 ttcagtacac tttgaacaaa ccagaaagct tctggaattc taagacaacc atgggtgtgc
    17221 ttgcgccagt taatgaagag tttttgaatt caaaaggaga tgattttgcc aaagctacgg
    17281 atccaagtag tctcttgtat aacggtcctt atttgttgaa atccattgtg accaaatctt
    17341 ctgttgaatt tgcgaaaaat ccgaactact gggataagga caatgtgcat attgacaaag
    17401 ttaaattgtc attctgggat ggtcaagata ccagcaaacc tgcagaaaac tttaaagatg
    17461 gtagccttac agcagctcgt ctctatccaa caagtgcaag tttcgcagag cttgagaaga
    17521 gtatgaagga caatattgtc tatactcaac aagactctat tacgtatcta gtcggtacaa
    17581 atattgaccg tcagtcctat aaatacacat ctaagaccag cgatgaacaa aaggcatcga
    17641 ctaaaaaggc tctcttaaac aaggatttcc gtcaggctat tgcctttggt tttgatcgta
    17701 cagcctatgc ctctcagttg aatggacaaa ctggagcaag caaaatctta cgtaatatct
    17761 ttgttccacc aacatttgtt caagcagatg gtaaaaactt tggcgatatg gtcaaagaga
    17821 aattggtcac ttatggggat gaatggaagg atgttaatct tgcagattct caggatggtc
    17881 tttacaatcc agaaaaagcc aaggctgaat ttgctaaagc taaatcagcc ttacaagcag
    17941 aaggtgtgac attcccaatt catttggata tgccagttga ccaaacagca actacaaaag
    18001 ttcagcgcgt ccaatctatg aaacaatcct tggaagcaac tttaggagct gataatgtca
    18061 ttattgatat ccaacaacta caaaaagacg aagtaaacaa tattacatat tttgctgaaa
    18121 atgctgctgg cgaagactgg gatttatcag ataatgtcgg ttggggtcca gactttgccg
    18181 atccatcaac ctaccttgat atcatcaaac catctgtagg agaaagtact aaaacatatt
    18241 tagggtttga ctcaggggaa gataatgtag ctgctaaaaa agtaggtcta tatgactacg
    18301 aaaaattggt tactgaggct ggtgatgagg ctacagatgt tgctaaacgc tatgataaat
    18361 acgctgcagc ccaagcttgg ttgacagata gtgctttgat tattccaact acatctcgta
    18421 cagggcgtcc aattttgtct aagatggtac catttacaat accatttgca ttgtcaggaa
    18481 ataaaggtac aagtgaacca gtcttgtata aatacttgga acttcaagac aaggcagtca
    18541 ctgtagatga ataccaaaaa gctcaggaaa aatggatgaa agaaaaagaa gagtctaata
    18601 aaaaggctca agaagatctc gcaaaacatg tgaaataact gttgcaaaat ataagaaagg
    18661 atttagtatt tctcttgaat gctgaatcct tttttacatt tgtaaagaaa gattctaaat
    18721 gtactgaccc ccaaaagttg gagcctcttt ttgtcagaat agagaaaatt tttgtta
//
LOCUS       DAVGJL010000023        18553 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.023, whole genome shotgun sequence.
ACCESSION   DAVGJL010000023 DAVGJL010000000
VERSION     DAVGJL010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18553
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.023"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            74..1867
                     /locus_tag="VVE42_000729"
     CDS             74..1867
                     /locus_tag="VVE42_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEU3072951.1"
                     /translation="MKKNNVEIIKADSLVRRRGDNVERHLKRVAAYCRVSSDSEDQKN
                     SYDSQVRHYKEYISQRSDWELADIYADEGISGTQVGKRQDFQRLINDCANGEIDYIVT
                     KAIARFARNTLDTLKYVRMLKDMQIGVYFEEENIDTLTMDGELLLTILSSVAQQEVEN
                     TSAHVKKGLKMKMQRGELVGFQGCLGYDYDVETKQISINKKEAKIVRYIFERYLEGIG
                     GKVIARELDELGYKSPRGLEHWNDTTVLGIIKNEKYKGDILMGKTFTVDPISKRRLSN
                     FGEEDKYYIKDNHEPIISKEDFEKAQEIRLRRAGNKKTAANVNGKRERYSKMYAFSSM
                     LECGFCGSILSRRSWHCRSDYRKVVWHCVTSIKKGKKFCKHSKGLEEIAIEGAFLEAY
                     RQVYHSNENLMTDLLETIESELNDNSLNKELKRITNKLRILLKKEENLVNLRLEGKVS
                     DSIYNEKYNEISSEKEFLAEEKVNIETTLKSEIDVKKRLTEFKHLLSSQKMLTEFDRA
                     VFESIVEKIIVGGVNSNGEIDPAMLTIIFKTGEIQNKDGKQFKSKRKNAKLETDKLCP
                     QNSDEDKKLYSQGTDNTRGVCSVAGSILASQ"
     gene            1972..2559
                     /locus_tag="VVE42_000730"
     CDS             1972..2559
                     /locus_tag="VVE42_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HEU3072952.1"
                     /translation="MGGNPPIKNYTIVNKIVSSRKIERIVIFTVFRENWEPYMKKYTE
                     VFQSQFPNLNIDYLLLDTEQIDLDSYLDADIIIIGGGNTEKYIATYVNQEFKSYIDHM
                     LNKEAKIIGFSAGALLLGEKVYVSPNDNSDHQIKIKNGLGLFSQFLISVHYDSWNDKA
                     NKDRAEELVNVPIIPLNDHSCLVLDKLGNIIEKID"
     gene            2840..3087
                     /locus_tag="VVE42_000731"
                     /pseudo
     CDS             2840..3087
                     /locus_tag="VVE42_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335280.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase"
     gene            3192..3494
                     /locus_tag="VVE42_000732"
                     /pseudo
     CDS             3192..3494
                     /locus_tag="VVE42_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            3650..4879
                     /locus_tag="VVE42_000733"
     CDS             3650..4879
                     /locus_tag="VVE42_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEU3072953.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGVTIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLIMYTVSGIAYRWFSTILALVSAASVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFVGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            4899..7595
                     /gene="ppc"
                     /locus_tag="VVE42_000734"
     CDS             4899..7595
                     /gene="ppc"
                     /locus_tag="VVE42_000734"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEU3072954.1"
                     /translation="MSLQKLENYSNKSVVQEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWYNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAKTLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATSLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITQKKSDTNTPNRYEAIMDQVVDRS
                     YDIYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYETILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     misc_binding    7678..7895
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8008..9012
                     /locus_tag="VVE42_000735"
     CDS             8008..9012
                     /locus_tag="VVE42_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU3072955.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEAQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATPAAQGLASATKDLPVIMAAITDPIGANLVKDLKKPGGNVTGVSDHNPAQQQ
                     VELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTSKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSTGKSVINKKIAQELGITIPESVLKEAGQVIE
                     "
     gene            9081..9947
                     /locus_tag="VVE42_000736"
     CDS             9081..9947
                     /locus_tag="VVE42_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU3072956.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQDVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGHMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            9944..10702
                     /locus_tag="VVE42_000737"
     CDS             9944..10702
                     /locus_tag="VVE42_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3072957.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFEQDFITILGGN
                     GAGKSTLFNTIAGTISLTSGTIRILGEDVTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLFPRRLASYKDKFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRVIQDLKQEEKAKMKISDYYQLFE"
     gene            10792..12552
                     /gene="dnaG"
                     /locus_tag="VVE42_000738"
     CDS             10792..12552
                     /gene="dnaG"
                     /locus_tag="VVE42_000738"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEU3072958.1"
                     /translation="MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVTFMEAVQILGQRVGIEVEKPLYSEQ
                     KPASPYQALYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKKSSGKTSEIYLMEGFMDVIAAYRAGI
                     ENAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAAILKALDEIGDMPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFIEKIAPLI
                     VQEKSIAAQNSYIHILADSLASFDYTQIEQIVNESRQVQRQNRMERISRPTPITMPVT
                     KQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEELERLISQKRRME"
     gene            12555..13664
                     /gene="rpoD"
                     /locus_tag="VVE42_000739"
     CDS             12555..13664
                     /gene="rpoD"
                     /locus_tag="VVE42_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEU3072959.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPVGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            13678..14007
                     /locus_tag="VVE42_000740"
     CDS             13678..14007
                     /locus_tag="VVE42_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEU3072960.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            14122..15165
                     /gene="cpoA"
                     /locus_tag="VVE42_000741"
     CDS             14122..15165
                     /gene="cpoA"
                     /locus_tag="VVE42_000741"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HEU3072961.1"
                     /translation="MEKKKLRINMLSSSEKVAGQGVSGAYRELVRLLHRAAKDQLIVT
                     ENLPIEADVTHFHTIDFPYYLSTFQKKRSGRKIGYVHFLPATLEGSLKIPFFLKGIVK
                     RYVFSFYNRMEHLVVVNPMFIEDLVAAGIPREKVTYIPNFVNKEKWHPLPQEEVVRLR
                     TDLGLSDNQFIVVGAGQVQKRKGIDDFIRLAEELPQITFIWAGGFSFGGMTDGYEHYK
                     KIMENPPKNLIFPGIVSPERMRELYALADLFLLPSYNELFPMTILEAASCEAPIMLRD
                     LDLYKVILEGNYRATAGREEMKEAILEYQANPAALKDLKEKAKNISREYSEEHLLQIW
                     LDFYEKQAALGRK"
     gene            15181..16506
                     /locus_tag="VVE42_000742"
     CDS             15181..16506
                     /locus_tag="VVE42_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU3072962.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGMLIRPSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLVAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGTLYYGE
                     HDLAGAILEALIATPDMNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFKTVLYLQQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            16537..16713
                     /locus_tag="VVE42_000743"
     CDS             16537..16713
                     /locus_tag="VVE42_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEU3072963.1"
                     /translation="MRSGRDKKIAGVCAGVAHYLDMDPTIVQVIWGVLTCCYGAGIVA
                     YIILWIIAPVATDY"
     gene            16772..16906
                     /locus_tag="VVE42_000744"
     CDS             16772..16906
                     /locus_tag="VVE42_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEU3072964.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            16970..18280
                     /gene="obgE"
                     /locus_tag="VVE42_000745"
     CDS             16970..18280
                     /gene="obgE"
                     /locus_tag="VVE42_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEU3072965.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVRVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEEFKKKLSENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEEAYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGVDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            18290..18448
                     /locus_tag="VVE42_000746"
     CDS             18290..18448
                     /locus_tag="VVE42_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3072966.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
BASE COUNT         5901 a         3086 c         4127 g         5439 t
ORIGIN      
        1 gtattaagta cacaaaatag ctgacttttt gatttgaatg cggtaatata gaatcaaaag
       61 gaggaatttc agtatgaaaa aaaataatgt tgaaataata aaagctgatt ctttagttcg
      121 tcgaagaggc gacaatgtag aaagacactt aaagagggtt gctgcttatt gcagagttag
      181 ttccgatagt gaggatcaga aaaacagtta tgattctcaa gtaagacact acaaagaata
      241 tatttctcag cgttcagatt gggagctggc agatatttat gcagatgaag gtatttcagg
      301 aacacaagta ggaaagagac aagattttca acgattaatc aatgattgtg caaatggaga
      361 gatagattat attgtcacaa aagcaattgc tagatttgca aggaataccc tagatacttt
      421 gaagtatgtc agaatgttaa aggatatgca aattggagta tattttgagg aagaaaacat
      481 agatacccta acaatggatg gagaattgct tctaactatt ctaagctccg ttgcacagca
      541 agaagtagag aatacttctg cccatgtgaa aaaaggatta aaaatgaaga tgcaacgtgg
      601 tgaattggtt ggttttcaag ggtgcttagg gtatgattat gatgtagaaa ccaagcagat
      661 ttcaattaat aaaaaagaag ccaaaattgt tcgttatatt tttgaaaggt atctagaagg
      721 tattggagga aaagtaattg caagggaact tgatgaactt ggttacaaat cgccaagagg
      781 tttagaacac tggaatgata caacagttct aggaataata aagaatgaaa aatataaagg
      841 tgatattcta atgggaaaaa cttttacagt tgacccaata agcaagagac ggttgagtaa
      901 ttttggagaa gaagataagt actatatcaa ggataatcat gaaccaataa tatctaaaga
      961 agattttgaa aaagctcagg agattagatt gcgtcgagca ggaaataaga agacagctgc
     1021 gaatgtaaat gggaagcgtg aacgctattc aaaaatgtat gcttttagta gcatgttgga
     1081 atgtggtttt tgtggttcaa tattatctag aagaagttgg cactgccgtt cagattatcg
     1141 caaagttgtg tggcactgtg ttacatcgat aaagaaagga aagaaatttt gtaagcatag
     1201 taaaggatta gaagagatcg ccattgaggg ggctttcctg gaagcttaca gacaagtcta
     1261 tcattcaaat gaaaatttaa tgacagactt acttgaaacg atagaatctg aattgaatga
     1321 caatagcctt aacaaggaac taaaaaggat tacaaacaaa cttcgaatat tactaaaaaa
     1381 agaggagaat cttgtaaatc taaggctcga aggaaaagtt agcgattcca tatacaatga
     1441 aaagtacaat gaaatttcct cagaaaaaga gtttctagca gaagagaagg taaatattga
     1501 aacaacatta aaatcagaaa tcgatgtaaa gaaaagacta actgagttta agcacttgct
     1561 atcttcgcaa aaaatgctta cggagtttga tcgtgcagtt tttgaaagta ttgttgagaa
     1621 aattattgta ggtggagtta atagcaacgg agaaattgat cctgcaatgt taaccataat
     1681 attcaaaaca ggtgaaattc aaaacaagga tggtaaacaa tttaaaagta aacgtaaaaa
     1741 tgctaaacta gaaacagata aattgtgtcc tcaaaacagt gacgaggata aaaaattgta
     1801 ttctcaagga acagacaaca cacgtggagt gtgtagtgtt gctggttcga tcttagccag
     1861 tcaataaaat gatatgtttt aaaaaaatta taatttcgaa acgtgaataa acttgtttaa
     1921 actttatgta tctttagaaa gtgtgataac ttgaaagaac aaattttttt gatgggtggg
     1981 aatccgccga taaagaacta tacgattgtt aataaaattg tgtcatcaag gaagatcgaa
     2041 agaattgtca tttttacagt ttttcgagaa aattgggaac cgtacatgaa aaagtacacg
     2101 gaagtttttc aaagtcaatt tcctaatcta aacattgatt acttgctcct ggacactgag
     2161 cagattgatc ttgatagcta tctagatgct gacataatta tcatcggtgg tggaaatacg
     2221 gaaaaatata ttgctactta tgttaatcag gagttcaaaa gttatatcga tcatatgctt
     2281 aataaagagg caaaaattat aggattttct gcaggagccc tactattagg agaaaaagtc
     2341 tatgtctcac ctaatgataa ttcagatcat cagataaaga taaaaaatgg attaggactt
     2401 tttagtcagt ttttaattag tgtccattat gattcctgga atgataaagc aaataaggat
     2461 agagctgaag aactcgttaa tgttcccata attccactaa atgatcattc ttgtcttgta
     2521 ttggataaac ttggaaacat catagagaaa attgactaac tttcataatg taaaaatgga
     2581 tctaggaaat aaaagtgtag acgaactgga taggttacta gtgaaaactg taaaaagtgt
     2641 tgtaaagtga atcggagtgt gcttgatgga ttttagtagt aagatagcca taaataaagg
     2701 ctggtcagat gataaaaaat attgtgtgac agttcaaaat cagtaaacat atttcttgcg
     2761 tgtttctgat aagtcgaagt tagattataa aaaaattgaa tttgatacga tggagaaagt
     2821 agcttctctt ggagttccga tgtgtaaacc aattagcatt gaactctgcg atggcgaagt
     2881 acattcttta cacgaatgga tagacggaag agatgcaata gatagcattt aacttattca
     2941 gaaaaccaac aatacacata tggtgtagaa gcaggaaaga tacttagaaa gattcataca
     3001 attcctgcta cagaagtttg tgaagattgg gaaatctttt ttaatctaaa aattgatgat
     3061 aaaatctcca acgaaatgat atggtaagtc cgaaacgctc agtgtgtaag cttgttgcta
     3121 aaagcgtaaa aaccttggaa cgaaaggaat aatagacttc ctgcgaaaca aaaatacaat
     3181 acaataaaac tatggatgat gaagcaagta aacaattaag cgatagccgt ttcaagatcc
     3241 ttgtaggtgt tcagcgcacg acttttgaag agatgttagc tgtgttaaaa acagcttatc
     3301 aacgtaaacg cgcaaaaggt ggacgaaaaa gcaaattaat cctagacgat ctccttatgg
     3361 taactattca atacatgcga gaatagagca cttatgaaca aattgcggct gattttggca
     3421 ttcacgaaag caacttaatc cgtcggagtc aatgggttga agcaactctt attcaaaatg
     3481 gttttacgat ttcaaattct gccttaattc tgtaaaaaca gtaaaattcg aaggattgta
     3541 aggtaagagt ttttttcttt ctgaaaaaat ggtataatag caatcaaaac tagaaaataa
     3601 aacggaattt ggaacagatt tatctgtatc ctagtagagt ggtgatgcta tgaagattag
     3661 taagaggcac ttattaaatt attccatctt gattccctac ttgcttttat ctattttggg
     3721 cttgattgtg gtctattcga ccaccagtgc tattttaatt gaagaaggca agagtgcctt
     3781 gcagttggtt cgaaaccaag gaatcttttg gattgttagt ttgatactga ttgccttaat
     3841 ttataaattg agactagatt ttttgagaaa tgagcgacta atcattttag ttatattaat
     3901 agaaatgctt ttattgttct tggctcgttt tattggtatt tccgtaaacg gggcatacgg
     3961 ttggatttcg gttgcaggag taactattca gccagctgag tacttaaaaa tcattattat
     4021 ttggtattta gctcaccgat tctccaaaca gcaagaagaa atagctactt atgattttca
     4081 agttttgact caaaatcaat ggcttccccg tgcttttaat gattggcgat tcgttctcct
     4141 agttctgatt ggaagtttgg gaattttccc tgatttagga aatgcgacta ttttagtctt
     4201 ggtttccttg attatgtata cagttagtgg aatcgcttat cgctggtttt caaccattct
     4261 ggcgctcgta tctgccgctt ctgtctttgt cttgaccact atcagcctaa tcggtgttga
     4321 gaccttttca aaaattccag tattcggcta tgtagccaag cgctttagtg ccttttttaa
     4381 tccttttgcc gatcgtgctg atgcaggtca ccagttagct aattcttatt ttgccatggt
     4441 caatggcggt tggtttggtc taggtcttgg aaactcgatt gaaaaacgag gttatttgcc
     4501 agaagctcat acagactttg tcttttctat cgtgattgaa gaatttggct ttgttggtgc
     4561 cagtcttatt ttagctctct tgtttttcat gattttgcgg attatcttgg tcggtatccg
     4621 agcggagaat cctttcaatg ccatggttgc actcggtgtc ggagggatga tgttggttca
     4681 ggtatttgtc aatatcggag ggatttcggg cttgattcca tctacaggag tgactttccc
     4741 cttcttatcc cagggtggaa atagtcttct agtcttatca gtggcagtag cctttgtctt
     4801 aaatattgat gccagtgaaa aacgcgctaa attgtaccga gaattggaaa atcaaccaat
     4861 gaaccttctg ttgaagtagg ataaagaaag gatagtttat gtctcttcaa aaattagaaa
     4921 attatagtaa taaaagtgtt gtgcaagaag aagtcttgat tctaacagaa ttactggaag
     4981 atattactaa aaatatgctt gccccagaga cctttgaaaa aataatacag ttgaaagaat
     5041 tatcaacgca ggaagattat caaggtctaa accgtctagt gactagctta tcaaatgatg
     5101 aaatggtcta tatttcacgc tatttctcta tcttgcctct tttgattaat atttcagagg
     5161 atgtggattt agcttatgaa atcaatcatc aaaataatat tgatcaggac tatttaggta
     5221 aattatctac aacgattaaa ttggtagcag aaaaggaaaa tgccgttgag atcctagaac
     5281 acttgaatgt tgtccctgtt ttgacagccc atccaacaca agtgcaacgc aaaagtatgt
     5341 tggatttaac aaatcatatt catagtcttt tgcgtaaata ccgtgatgtt aagttggggt
     5401 tgatcaataa agataaatgg tacaatgatt tgcgtcgtta catcgaaatt atcatgcaga
     5461 cagacatgat tcgtgagaaa aaattaaaag tgactaacga aatcacgaat gctatggaat
     5521 attataacag ctcctttttg aaagctgtac ctcatttgac gacggagtat aagcgcttag
     5581 cgcaagcgca tggtctgaat ttaaaacagg ctaaaccaat caccatgggt atgtggatag
     5641 gtggtgaccg tgatggaaat ccatttgtta cagcaaagac cttgaagcag tctgcactca
     5701 ctcagtgtga agtcatcatg aactactatg ataaaaagat ttaccaactt tatcgtgaat
     5761 tttctctttc aactagcatt gtcaacgtca gcaagcaagt cagagaaatg gctcgtcaat
     5821 ccaaggataa ctcgatttac cgcgaaaaag agctttaccg tcgtgccttg tttgatattc
     5881 aatcaaaaat tcaggcaact aaaacctatc tgattgagga tgaagaagtt gggactcgtt
     5941 atgaaaccgc caatgatttc tacaaggatt tgattgccat tcgagattct ctactagaaa
     6001 ataagggcga gtccttgatt tcaggtgatt ttgtggaatt attgcaggca gtagagatat
     6061 ttggttttta cttagcatca attgatatgc gacaagactc tagcgtctat gaagcctgtg
     6121 tggcagaact cttgaaatca gcaggaattc attctcgtta tagcgagttg agcgaagaag
     6181 aaaagtgtga ccttctcttg aaagaattag aagaagatcc ccgaattctt tctgcgactc
     6241 acgcagaaaa atcagaatta ttagcaaaag aattagctat ttttaagacg gctcgtgttt
     6301 tgaaagataa gttgggagat gatgtcatcc gtcagaccat catttcacat gcaaccagcc
     6361 tttctgatat gctagaatta gctattctgt taaaagaagt aggactggtg gatacggaaa
     6421 gggcgcgtgt tcagattgtt cccctttttg aaacaattga agacttggat cattcagagg
     6481 aaacaatgag aaaatatctt tctcttagcc ttgccaaaaa atggattgac tcacgaaata
     6541 actaccaaga aatcatgctt ggctactctg acagtaataa agatggcggt tacttgtcat
     6601 catgttggac cctctacaag gctcaacaac aattgactgc tattggagat gaatttggcg
     6661 ttaaggttac cttcttccat ggtcgtggtg gtactgtcgg tcgtggtggt gggccaacct
     6721 atgaagccat tacatctcaa ccgctcaagt ctatcaagga tcgtatccgc ttgacggagc
     6781 agggtgaagt aattgggaat aaatacggta acaaagacgc cgcttactat aaccttgaaa
     6841 tgctagtatc ggcagctatt aaccgtatga ttactcagaa gaagagcgat accaataccc
     6901 caaatcgtta tgaagccatt atggatcaag tagtggaccg tagttacgat atataccgtg
     6961 atttggtctt tggtaatgag catttctatg attatttctt cgagtcaagt ccaatcaagg
     7021 ctatttcaag ttttaatatt ggttctcgtc cagccgctcg taagactatt actgaaatcg
     7081 gtggtttgcg tgccatccct tgggtattct catggtcaca gagtcgtgtt atgttccctg
     7141 gatggtacgg ggttggttca agcttcaagg aatttatcaa taaaaatcca gagaatattg
     7201 ctatcttacg agatatgtac caaaattggc ctttcttcca atcgcttctt tcaaatgttg
     7261 atatggtttt gtcaaaatca aatatgaata ttgcttttga atatgctaaa ctttgtgaag
     7321 acgagcaagt taaggccatc tatgagacta ttttaaatga atggcaagtt actaagaacg
     7381 ttatcttggc tattgaagga catgacgaac tcttagctga caatccatat ctaaaagcta
     7441 gtctggatta ccgtatgcct tactttaata ttctcaacta tattcagtta gagttgatta
     7501 aacgccaacg tcgtggagaa ttgtccagtg atcaagaacg attgattcat atcaccatca
     7561 acggaattgc gacaggattg cgtaattcag gttgataatt ttcaagagtg aatgctaaaa
     7621 gtgaatatca aaaaaattct aatagactat tgacaagtag tttaaaaatg atataattta
     7681 accattcaga aaagtaatca tacaaacttt ttagagagtc tgtggtagct gaaaacagat
     7741 aagtggcaat gatgaaaatt gggctgaatg ctatttagaa tttgaaatta taaaaattcg
     7801 gtaagcacac cttacagtgc atctcgttat tgcgagactg agcgataggg aaattcccta
     7861 taattgaggt ggtaccgcgc atcgacgtcc tcacacaagt tttttgtgtg aggatttttt
     7921 tgatggaggt tagtatggaa agaaaacaat ggcgtcgctt gtttagataa gtgaaatatg
     7981 ttaaaggaaa taaaaaggag aaacagaatg aaaaataaac gtttaattgg aattattgct
     8041 gcattagcag tcttagtagc aggaagcttg atttattctt caatgaataa atcagaagct
     8101 cagaataata aggatgagaa gaaaataacc aagattggtg tgcttcaatt tgtgagccat
     8161 ccatcccttg atttgattta taaagggatc caagatggac ttgcagaaga aggatataaa
     8221 gatgatcaag ttaaaattga ttttatgaac tcagaaggtg accaaagtaa ggttgcgaca
     8281 atgagtaaac aattggttgc aaatgggaat gaccttgtgg ttggtatcgc aacaccagca
     8341 gcccaagggt tggctagtgc aacaaaagac ctaccggtta tcatggccgc tattacagac
     8401 ccaattggtg ctaacttggt taaagatttg aaaaaaccag gtggcaacgt tacaggggta
     8461 tctgaccaca atccagctca acaacaagtt gaactcatca aggctctgac accgaatgtg
     8521 aaaacaatcg gagctcttta ctcaagtagc gaagacaatt caaaaacaca ggtcgaagaa
     8581 tttaaggctt atgctgaaaa agcaggtctg acagtggaaa catttgcagt tccttcaaca
     8641 aatgaaattg cctcaactgt cactgttatg actagcaagg tagatgctat ttgggttcca
     8701 attgataaca ccattgcatc aggatttcca acggttgtct ctagcaatca aagttctaag
     8761 aaaccaattt atcccagtgc gacagctatg gtagaagtag gtggtttggc atcagttgta
     8821 attgaccaac atgaccttgg tgtggcaaca ggtaaaatga ttgtgcaagt cttgaaaggt
     8881 gcaaaaccag ccgatacccc agtcaatgtc ttttcaactg gtaagtcagt catcaataaa
     8941 aaaatagcac aagaactagg tattactatt cctgagtctg ttctcaaaga agcaggacaa
     9001 gtcatcgaat aaataaggta gggaggagga gactcttccc attttttaaa aagatgaata
     9061 tagaaaggat taaataaaat atgattgttt ccattatttc tcaaggattt gtctgggcta
     9121 ttctaggtct gggaatcttt atgacattta ggattttaaa ctttccagat atgacgacag
     9181 aaggttcctt ccctcttggg ggagctgttg ctgtcacttt gataaccaaa ggcgtgaacc
     9241 catttttagc gacacttgtt gctgtaggag caggttgttt ggctggaatg gcagcaggcc
     9301 ttctttatac aaaagggaag atcccaacct tgctctcagg gattttggtg atgacttctt
     9361 gtcactcaat catgctcttg attatgggac gtgcgaattt aggcctgctt ggaaccaagc
     9421 aaattcagga tgttttgcct tttgattcgg atttgaatca actcttgaca ggtctcatct
     9481 ttgtgagtat tgttattgct ctcatgctct ttttcttgga cactaaactc ggacaagcct
     9541 atattgctac aggggataat cctgatatgg ctagaagttt cgggattcat actggacaca
     9601 tggagctcat gggcttggtc ttatcaaatg gtgtgattgc ccttgcaggt gccctcattg
     9661 ctcagcaaga aggttatgcc gatgtgtctc gagggatcgg ggttatcgtt gtggggcttg
     9721 caagtttgat tattggagaa gttattttca agagtttgag cttggcagag cgtttggtta
     9781 ctatcgttgt aggttctatc gcttatcaat ttttagtgtg ggcagttatc gcacttggct
     9841 ttaatacaag ttaccttcgt ttatacagtg ccttgatttt agcagtctgc ctcatgattc
     9901 caacatttaa gcaaacaatc ttgaaaggag ccaagttaag caaatgacag caattgtaga
     9961 attaaaaaat gcaaccaaaa tcgttaaaaa tggctttgat gaagaaaaga ttattttaaa
    10021 tgatgtttcc ttagaaattt ttgaacagga ctttatcacg attttgggtg gaaatggtgc
    10081 tggtaaatca accctcttta acactattgc aggaaccata tcactaacca gtggaactat
    10141 ccgtatttta ggtgaggatg ttaccaagtt ttcaccagag aagcgcgcta agtatctgtc
    10201 tcgagtcttc caagatccaa agatggggac agctcctcgt atgacagtgg cagagaatct
    10261 cctgattgcc aagtttcgtg gtgaaaagcg tggactattt ccgagacgtc tggctagcta
    10321 taaggataag tttcaggcga ccattgaaaa agtgggaaac ggtcttgaga aacacttgaa
    10381 tacaccgatt gagttcttat ctggtggaca aagacaggct ttgagtctct tgatggcaac
    10441 cttgaagcga cctgaattac tcttgttaga cgagcatact gctgccctgg atccaaagac
    10501 tagtgttgct ttgatggaat tgacagatga atttgttaag aaagatcaat tgacagccct
    10561 tatgattacc catcatatgg aagatgctct caaatatggc aatcgcttga ttgtcatgaa
    10621 agaaggacgg gttatccaag atttgaaaca agaagaaaaa gcaaaaatga aaatctctga
    10681 ttattatcaa ctctttgaat aaagtaggga aaatgagtga aatggtttac tttttttctg
    10741 aaataaagta tactatataa agtaaactat gataacatgg aggtattgtg tatggttgac
    10801 aaacaagtca ttgaagaaat caaaaacaat gccaacattg tggaagtcat aggagatgtg
    10861 atttctttac aaaaggcagg acggaactat ctagggctct gtccttttca tggtgaaaaa
    10921 acaccttctt tcaacgttgt agaggacaag cagttttacc actgttttgg ttgtggtcgc
    10981 tcaggtgatg tctttaaatt catcgaggag taccaagggg ttacctttat ggaggctgtc
    11041 caaatcttag gtcagcgtgt cgggattgag gttgaaaaac cgctttatag tgaacagaag
    11101 ccagcctcgc cttaccaagc tctttatgat atgcacgaag atgcggctaa attttaccat
    11161 gctattctca tgacaacgac tatgggcgaa gaggccagaa attaccttta tcagcggggt
    11221 ttgacagatg aagtgcttaa acatttttgg attggtttag cacctccaga acgaaactat
    11281 ctctatcaac gtttgtctga tcagtatcgt gaagaggatt tactggattc aggcctgttt
    11341 tatctctcgg atgccaatca atttgtagac acctttcaca atcgcattat gtttcccctg
    11401 acaaatgacc aaggaaaggt cattgccttc tcaggtcgta tctggcaaaa aacggattca
    11461 caaacttcta agtataaaaa cagccgatcg actgcaattt ttaacaaaag ttacgaatta
    11521 tatcatatgg atagggcaaa aaaatcttct ggaaaaacta gtgagattta cctgatggaa
    11581 ggattcatgg atgttattgc agcctatcgg gctggaatcg aaaatgctgt ggcgtcgatg
    11641 ggaacggcct tgagtcgaga gcatgttgag catctgaaaa ggttaaccaa gaaattggtt
    11701 cttgtttacg atggagataa ggctgggcaa gccgcgatat tgaaagcatt ggatgaaatt
    11761 ggtgatatgc ctgtgcaaat cgttagcatg cctgataact tggatcctga tgagtatcta
    11821 caaaaaaatg gtccagaaga cttggcctat ctattaacaa aaactcgtat tagtccgatt
    11881 gagttctaca ttcatcagta caaacctgaa aacagtgaaa atctgcaggc tcagattgag
    11941 tttattgaaa aaatagctcc cttgattgtt caagaaaagt ccatcgctgc tcaaaacagc
    12001 tatattcata ttttagctga cagtctggcg tcctttgatt atacccagat tgagcagatt
    12061 gttaatgaga gtcgtcaggt gcaaaggcag aatcgcatgg aaagaatttc cagaccgacg
    12121 ccaatcacca tgcctgtcac caagcagtta tcggctatta tgagggcaga agcccatcta
    12181 ctctatcgga tgatggaatc ccctcttgtt ttgaacgatt accgtttgcg agaagacttt
    12241 gcatttgcta cacctgaatt tcaggtctta tatgacttgc ttggccagta tggaaatctt
    12301 cctccagaag ttttagcaga gcagacagag gaagttgaaa gagcttggta ccaagtttta
    12361 gctcaggatt tgcctgctga gatatcgccg caggaactta gtgaagtaga gatgactcga
    12421 aacaaggctc tcttgaatca ggacaatatg agaatcaaaa agaaggtgca ggaagctagc
    12481 catgtaggag atacagatac agccctagaa gaattggaac gtttaatttc ccaaaagaga
    12541 agaatggagt aataatggca acaaaacaaa aagaagtaac aacatttgac gtacaggtag
    12601 cagaatttat ccgtaatcat aagcaaaaag ggacagcaac agatgatgaa atcaatgcaa
    12661 gtctggttat tccttttacc ttggacgctg atgggattga agatctcttg caacggattc
    12721 aggatgcagg catttctatc acagataacg aaggaaatcc aagtgcgcgt gttcttagca
    12781 atgaagaaga accagaactc agcgatgagg acttgattgg gtcaacttct gctaaggtca
    12841 atgaccctgt ccgtatgtac ttgaaagaaa taggggtcgt tcctctcttg accaatgaag
    12901 aggagaaaga gttggcactg gctgttgaag ctggcgatat cgaagccaaa caacgtcttg
    12961 cggaagccaa tcttcgtttg gttgtttcca ttgccaaacg ctatgtcggt cgtggcatgc
    13021 agttccttga cttgattcaa gaaggaaata tgggcttgat gaaggcggtt gacaagtttg
    13081 actattctaa agggttcaag ttttcaactt atgcaacttg gtggattcgt caggctatca
    13141 ctcgtgctat tgcggaccaa gctcgtacca tccgtatccc agttcacatg gttgaaacta
    13201 tcaataaatt ggttcgtgaa cagcggaatc tccttcaaga attggggcaa gatccgacac
    13261 cagaacagat tgctgaacga atggatatga cacctgataa ggttcgtgaa atcttgaaga
    13321 ttgcccaaga accagtatct cttgaaactc ctgtcggtga agaggacgat agccaccttg
    13381 gagactttat cgaagatgaa gtgattgaaa atccagtgga ttatacgact cgtatcgtct
    13441 tgcgtgagca attggatgaa atcttagata ctcttacaga ccgtgaagaa aatgttctgc
    13501 gtctacgttt tggactagat gatggaaaaa tgcgcacact tgaagatgtg gggaaagtct
    13561 ttaacgtaac tcgtgagcgt atccgtcaga ttgaagcaaa ggctttgaga aaactacgcc
    13621 aaccaagtcg cagcaaaccg cttcgtgatt ttattgaaga ctaagagtga ggaaaatatg
    13681 gcttatacag aagagcaaat tgaaaacatc aaaacacgga ttttaacagc cttggaagaa
    13741 gtcatcgacc ctgagttggg aatcgatatt gtcaatcttg gtttgatcta tgagattcgt
    13801 tttgacggtg acacagggca aacagagatt gacatgactt tgacaactat gggttgtccc
    13861 ttagcagacc ttttgacaga ccagatttat gatgctatga tagaggttcc agaagtaacg
    13921 gatactgagg ttaaattagt ctggtatcca gcctggacag ttgaaaaaat gagtcgctat
    13981 gcccgcattg ccctaggcat taagtaaaca tataaaagca tgtgagagac tgttggaaaa
    14041 gcgaggaaat ttcccctctt ttcctctagt ctctcctttc ttttgctgat tttattcaaa
    14101 gaaaatgata taatagtagt tatggagaaa aagaaattac gcatcaatat gttgagttca
    14161 agtgagaaag tagcaggaca gggagtttca ggtgcttacc gtgaattagt tcgtcttctt
    14221 caccgtgctg ccaaggacca attgattgtt acagaaaatc ttccgatcga ggcagatgtg
    14281 actcactttc atacgattga ttttccctat tatttatcaa ccttccaaaa gaaacgctca
    14341 gggagaaaga ttggctatgt gcatttcttg ccagctacac ttgagggaag tttgaaaatt
    14401 ccatttttct taaagggaat tgtgaaacgc tatgtatttt ctttttacaa ccggatggag
    14461 cacttggttg tggtcaatcc tatgtttatt gaggatttgg tagcagctgg tattccacgt
    14521 gaaaaagtga cctatattcc taactttgtc aacaaggaaa aatggcatcc tctaccacaa
    14581 gaagaggtag tcagactgcg cacagatctt ggtcttagtg acaatcagtt tatcgtagta
    14641 ggtgctgggc aagttcagaa acgtaaaggg attgatgact ttatccgtct ggctgaggaa
    14701 ttgcctcaga ttacctttat ctgggctggt ggcttctctt ttggtggtat gacagatggt
    14761 tatgaacact ataagaaaat tatggaaaat ccccctaaaa atttgatttt tccaggcatt
    14821 gtatcgccag agcggatgcg cgaattgtat gctctagcgg atcttttctt gttgcctagt
    14881 tacaatgagc tctttcctat gactatttta gaagctgcga gttgtgaggc tcctattatg
    14941 ttgcgtgatt tagatctcta taaggtgatt ttggagggaa attatcgggc gacagcgggt
    15001 agagaagaga tgaaagaggc tattttggaa tatcaagcaa atcctgctgc cttaaaagat
    15061 ctcaaagaaa aggctaagaa tatttccaga gagtattctg aagagcatct gttacaaatc
    15121 tggttggact tttatgagaa acaagccgct ttagggagaa agtaaaaagt gaggtaatct
    15181 atgcgaattg gtttatttac agatacctat tttcctcagg tttctggtgt tgcgaccagt
    15241 attcgaacct tgaaaacaga acttgaaaag cagggacatg ctgtttttat ctttacgacg
    15301 acagataagg atgtcaatcg ctacgaagat tggcaaatta tccgcattcc aagtgttcct
    15361 ttctttgctt ttaaggatcg tcgctttgcc taccgaggtt ttagcaaggc acttgaaatt
    15421 gctaaacagt atcagctaga tattatccat actcagacag aattttctct tggcctgttg
    15481 gggatttgga ttgcgcgtga attgaaaatt ccagtcatcc atacctatca cacccagtat
    15541 gaagactatg tccattatat tgctaagggg atgttgatcc gaccgagtat ggtcaagtat
    15601 ctggttagag gtttcctgca tgatgtggat ggggttattt gccctagtga gattgtccgt
    15661 gacttgctat ctgattataa ggtcaaggtt gaaaaacggg tcattcctac tgggattgaa
    15721 ttagccaagt ttgagcgtcc ggaaatcaag caggaaaatt tgaaagaact gcgtagtaaa
    15781 ctagggattc aagatggtga aaagacgttg cttagtcttt cgagaatctc ctatgaaaaa
    15841 aatattcaag cagttttagt agcctttgct gatgttctga aagaggaaga caaggttaaa
    15901 ctggtagtag ctggggatgg cccttatctg aatgacctca aagagcaagc ccagaaccta
    15961 gagattcaag actcagtcat ctttacaggg atgattgctc ctagtgagac ggctctttac
    16021 tataaagcgg cggatttttt catttcggca tcgacaagcg aaacgcaagg tttgacctac
    16081 ttggaaagct tagccagtgg aacacctgtc attgctcacg gaaatcctta tttgaacaac
    16141 ctcatcagtg ataaaatgtt tggaaccttg tactatggag aacatgattt ggctggtgct
    16201 attttggaag ccctgattgc aacaccagac atgaacgagc ataccttatc agagaaattg
    16261 tatgagattt cagctgagaa ctttgggaaa cgagtgcatg agttttatct ggatgccatt
    16321 atttcaaata acttccagaa agatttggct aaagatgata cggtcagtca gcgtatcttt
    16381 aagacagttt tgtatcttca gcaacaggtg gttgctgtac ctgtaaaagg atctagacgc
    16441 atgttgaagg cttcaaaaac acagttgatc agtatgagag actattggaa agaccatgaa
    16501 gaatagaaag aggaacagct atgaaaaaaa caattaatga gaagcggtcg tgataaaaag
    16561 attgcgggtg tttgtgctgg ggtggcccat tatctggata tggatccgac tatcgttcaa
    16621 gtcatttggg gtgttcttac ttgctgttac ggagctggaa ttgtagctta cattatttta
    16681 tggattatcg caccagtagc aactgactat tgaaaactag ctcaattcat gctaagataa
    16741 tagaaaatag aatgtaacga aggagagaaa aatggcattt ggagataatg gaaatcgtaa
    16801 aaaaactatg tttgagaaaa taaccttgtt tatcgtgatt atcatgctag tagcaagttt
    16861 attgggaatt tttgcaactg caattggtgc cctcagtaat ctataaaata gattcaagaa
    16921 aatttagtga ctgggatttc ccagcccttt tttaaagtga gaagaaataa tgagtatgtt
    16981 tttagataca gctaagatta aggtcaaggc tggtaatggt ggcgatggta tggttgcctt
    17041 tcgtcgtgaa aaatatgtcc ctaatggagg cccttggggt ggtgatggtg gtcgtggagg
    17101 caatgtggtc ttcgttgtag acgaaggact acgtaccttg atggatttcc gctacaatcg
    17161 tcatttcaag gctgattctg gtgaaaaagg gatgaccaaa gggatgcatg gtcgtggtgc
    17221 tgaggacctt agagttcgag taccacaagg tacgactgtt cgtgatgcgg agactggcaa
    17281 ggttttaaca gatttgattg aacatgggca agaatttatc gttgcccacg gtggtcgtgg
    17341 tggacgtgga aatattcgtt tcgcgacacc aaaaaatcct gcaccggaaa tctctgaaaa
    17401 tggagaacca ggtcaggaac gtgagttaca attggaacta aaaatcttgg cagatgtcgg
    17461 tttagtagga ttcccatctg tagggaagtc aacactttta agtgttatta cctcagctaa
    17521 gcctaaaatt ggtgcctacc actttaccac tattgtacca aatttaggta tggttcgcac
    17581 ccaatcaggt gaatcctttg cagtagccga cttgccaggt ttgattgaag gggctagtca
    17641 aggtgttggt ttgggaactc agtttctccg tcacatcgag cgtacacgcg ttatccttca
    17701 catcattgat atgtcagcta gcgaaggccg tgatccatat gaggattacc tagctatcaa
    17761 taaagagctg gagtcttaca atcttcgcct catggagcgt ccacagatta ttgtagctaa
    17821 taagatggat atgcctgaga gtcaggaaaa tcttgaagaa tttaagaaaa aattgtctga
    17881 aaattatgat gaatttgaag agttaccagc tatcttccca atttctggat tgaccaagca
    17941 aggtctggca acacttttag atgctacagc tgaattgtta gacaagacac cagaattttt
    18001 gctctacgac gagtccgata tggaagaaga agcttactat ggatttgacg aagaagaaaa
    18061 agcctttgaa attagtcgtg atgacgatgc gacatgggta ctttctggtg aaaaactcat
    18121 gaaactcttt aatatgacca actttgatcg tgatgaatct gtcatgaaat ttgcccgtca
    18181 gcttcgtggt atgggggttg atgaagccct tcgtgcgcgt ggagctaaag atggggattt
    18241 ggtccgcatt ggtaaatttg agtttgaatt tgtagactag gagactggta tgggagataa
    18301 accgatatct ttccgagatg cggatggtaa ttttgtttcc gccgcagacg tttggaatga
    18361 aaagaaattg gaagaactat ttaatcgtct caatccaaat cgtgccttga gattggcacg
    18421 aactaaaaag gaaaatccat ctcagtaaag aagctaaaaa atcccggctc tttgtcaact
    18481 gtagtgggtt gaaaaaaagc taagcttgag aaaggacaaa ttttgtcctt tcttttttga
    18541 tgttcagagc gat
//
LOCUS       DAVGJL010000024        18301 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.024, whole genome shotgun sequence.
ACCESSION   DAVGJL010000024 DAVGJL010000000
VERSION     DAVGJL010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18301
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.024"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            626..1078
                     /locus_tag="VVE42_000747"
     CDS             626..1078
                     /locus_tag="VVE42_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEU3072967.1"
                     /translation="MIITKISRLGTYVGVNPHFATLIDFLEKTGLENLTEGSIAIDGN
                     RLFGNCFTYLADGQAGAFFETHQKYLDIHLVLENEEAMAVTSPENVSVTQEYDEGKDI
                     ELYTGKVEQLVHLRAGECLITFPEDLHQPKVRINDEPVKKVVFKVAIS"
     gene            1097..2434
                     /locus_tag="VVE42_000748"
     CDS             1097..2434
                     /locus_tag="VVE42_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEU3072968.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQVMWV
                     RTDLLKEHNIEVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDKDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLDSTPVGMLPTIKGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            2513..3397
                     /locus_tag="VVE42_000749"
     CDS             2513..3397
                     /locus_tag="VVE42_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU3072969.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKAVLSDPNFFNAFFNSIKWTVFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWILNGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            3397..4230
                     /locus_tag="VVE42_000750"
     CDS             3397..4230
                     /locus_tag="VVE42_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU3072970.1"
                     /translation="MKKKSSIYLDILSHVLLVGATIVAIFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            4248..6341
                     /gene="nanB"
                     /locus_tag="VVE42_000751"
     CDS             4248..6341
                     /gene="nanB"
                     /locus_tag="VVE42_000751"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase NanB"
                     /protein_id="HEU3072971.1"
                     /translation="MNKRGLYSKLGISVVGISLLMGVPTLIHANELNYGQLSISPIFQ
                     GGSYQLNNKSIDISPLLLDKLSGESQTVVMKFKADEPNSLQALFGLSNSKAGFKNNYF
                     SIFMRDSGEIGVEIRDAQKGINYLFSRPASLWGKHKGQAVENTLVFVSDSKDKTYTMY
                     VNGIEVFSETVDTFLPISNINGIDKATLGAVNREGKEHYLAKGSIDEISLFNKAISDQ
                     EVSNIPLSNPFQLIFQSGDSTQANYFRIPTLYTLSSGRVLSSIDARYGGTHDSKSKIN
                     IATSYSDDNGKTWSEPIFAMKFNDYEEQLVYWPRDNKLKNSQISGSASFIDSSIVEDK
                     KSGKTILLADVMPAGIGNNNANKADSGFKEINGHYYLKLKKNGDNDFRYTVRENGVVY
                     DETTNKPTNYTINDKYEVLEGGKSLTVEQYSVDFDSGSLRERHNGKQVPMNVFYKDSL
                     FKVTPTNYIAMTTSQNRGESWEQFKLLPPFLGEKHNGTYLCPGQGLALKSSNRLIFAT
                     YTSGELTYLISDDSGQTWKKSSASILFKNATAEAQMVELRDGVIRTFFRTTTGKIAYM
                     TSRDSGETWSEVSYIDGIQQTSYGTQVSAIKYSQLIDGKEAVILSTPNSRSGRKGGQL
                     VVGLVNKEDDSIDWKYHYDIDLPSYGYAYSAITELPNHHIGVLFEKYDSWSRNELHLS
                     NVVQYIDLEINDLTK"
     gene            6353..7456
                     /locus_tag="VVE42_000752"
     CDS             6353..7456
                     /locus_tag="VVE42_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU3072972.1"
                     /translation="MVKYGVVGAGYFGAELARYMQKNDGAEITLLYDPDNAEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQHGKNVFCEKPIALSYQDCRE
                     MVDACKENNVTFMAGHIMNFFNGVHHAKELINQGVIGDVLYCHTARNGWEEQQPSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVAHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRTRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMEGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVKLSEIVK"
     gene            7629..8327
                     /locus_tag="VVE42_000753"
     CDS             7629..8327
                     /locus_tag="VVE42_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEU3072973.1"
                     /translation="MPQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAA
                     EQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIE
                     VIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTL
                     SGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAIT
                     RPKEITERFVASLK"
     gene            8346..9863
                     /locus_tag="VVE42_000754"
     CDS             8346..9863
                     /locus_tag="VVE42_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEU3072974.1"
                     /translation="MFKVLQKVGKAFMLPIAILPAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGTAIDTGVIGALAVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETIAGQTVVGAQKIFFAQLADPAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFFGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPIGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNTADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            9988..11316
                     /locus_tag="VVE42_000755"
     CDS             9988..11316
                     /locus_tag="VVE42_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU3072975.1"
                     /translation="MKFRKLACTVLAGAAVLGLAACGNSGGSKDAAKSGGDGAKTEIT
                     WWAFPVFTQEKTGDGVGTYEKSIIEAFEKANPDIKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            11568..12434
                     /locus_tag="VVE42_000756"
     CDS             11568..12434
                     /locus_tag="VVE42_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU3072976.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAVMIAIVSF
                     VQFKVLGNDVEY"
     gene            12450..13289
                     /locus_tag="VVE42_000757"
     CDS             12450..13289
                     /locus_tag="VVE42_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU3072977.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            13561..14013
                     /locus_tag="VVE42_000758"
     CDS             13561..14013
                     /locus_tag="VVE42_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEU3072978.1"
                     /translation="MIFDDLKNITFYKGIHPNLDKAIDYLYQHRKDSFELGKYEIDGD
                     KVFLVVQENVLNQVENNRFEHHKNYADLHLLIEGHEYSSYGSRIKDEAVAFDEASDIG
                     FVHCHEHYPLLLGYHNFAIFFPGEPHQPNGYAGMEEKVRKYLFKILID"
     gene            14110..14454
                     /locus_tag="VVE42_000759"
     CDS             14110..14454
                     /locus_tag="VVE42_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3072979.1"
                     /translation="MVSSKYAARASFLDGQGITVDEMAWIIRGIVTALIGRYIKLGTY
                     AVKYGISMARSILSRAAATAAARVGLLTKIFGWILRIAVLAADVYGNFANNIAVAWDA
                     HDKIPNNGRINF"
     gene            14741..15385
                     /locus_tag="VVE42_000760"
     CDS             14741..15385
                     /locus_tag="VVE42_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEU3072980.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVVSCLPIV
                     TIGVAKISLYETMFEVKKSRRVPVFKIYLRSFKQNLKLGLQLGLMELGIVFLTLSDLY
                     LFWGQTALPFQLLKAICLGILIFLTIVMLASYPIAARYDLTWKEVLQKGLILASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSVFLLFGFGLLVFIQTGLMEKIFAKYQ"
     gene            15554..16471
                     /locus_tag="VVE42_000761"
     CDS             15554..16471
                     /locus_tag="VVE42_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU3072981.1"
                     /translation="MSDLKKYEGVIPAFYACYDDQGEVSPERTRALVQYFIDKGVQGL
                     YVNGSSGECIYQSVEDRKLILEEVMAVAKDKLTIIAHVACNNTKDSMELARHAESLGV
                     DAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEMLKN
                     PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGARAGIGGTYGAMPE
                     LFLKLNQLIADKDLETARELQYAINAIIGKLTSAHGNMYGVIKEVLKINEGLNIGSVR
                     SPLTPVTEEDRPVVEAAAALIRETKERFL"
     gene            16489..17373
                     /locus_tag="VVE42_000762"
     CDS             16489..17373
                     /locus_tag="VVE42_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU3072982.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASVTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFKEATEGNKICMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAHH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(17389..18240)
                     /locus_tag="VVE42_000763"
     CDS             complement(17389..18240)
                     /locus_tag="VVE42_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEU3072983.1"
                     /translation="MDKPDIATIIDSHFEEMTDLEQEIARYFLQAETITDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHQAENQANQVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEIQLERIAQLIEDAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDENCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFTSVPNKDSQTYTET
                     VLVATHSQPSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
BASE COUNT         5768 a         3093 c         3837 g         5603 t
ORIGIN      
        1 taaattccaa attagaaaaa gacttgaaat actaaaaaaa aaaagtatac tctaattgaa
       61 aacggtaaca aaactaattt agagaatgaa atatagagta tttctctctt aaaagttttt
      121 ggtgaaacga gatgtagaaa ggagatttag ccaaagagtc tattagtgct agaataatag
      181 attagaatta ttttagaaaa acgaagtgag cagcttataa attcaagtcc ccaaatagat
      241 tcatactagt atcttttgca aaaaataaag ggcgacttcc ttcatgaata tcaatttcat
      301 ctataaggaa ggtagctaat tgaactaact tatttattct gtttgtcgct agaaaaatca
      361 gacctccttg tgaagattga ggagatactt aatgaaaatc aaagaagaaa ctagcaagct
      421 agtagcagat tgcccaaaac accgctttga ggttgtagat aagactgacc tatataatcc
      481 aaggtgaagc gactgtggtt tgaagagatt ttcaaagagt ataggctaga gagtagtgtt
      541 tttatgtcct tctagtagaa aatgctagac agaagaatgg ggaacttgga taggaaaaat
      601 agattgagaa aggaggttag aagagatgat tattacaaaa attagccgtt taggaactta
      661 tgtgggagta aatccacatt ttgcaacatt aatagatttt ctagaaaaaa caggactaga
      721 aaatttaaca gaaggttcga ttgctatcga tggtaatcga ttgtttggga attgctttac
      781 ttatctagca gatggtcaag caggggcttt ctttgaaaca caccaaaaat atttggatat
      841 tcatttagtt ttggaaaacg aagaagccat ggctgttaca tcgccggaaa atgtaagcgt
      901 tacccaagaa tatgatgaag ggaaagatat tgaattatac acagggaaag tggaacagtt
      961 ggttcatttg agagctggcg aatgcctcat cacttttcca gaagatttac atcaacccaa
     1021 ggttcgtata aatgatgaac ctgtgaaaaa agttgtcttt aaagttgcga tttcttaatg
     1081 tagaaagaga agaacgatga aaaaaatgag aaagttttta tgtctagctg gaattgcgct
     1141 agcggctgtt gccttggtag cttgttcagg aaaaaaagaa gctacaacta gtactgaacc
     1201 accaacagaa ttatctggtg agattacaat gtggcactcc tttactcaag gaccccgttt
     1261 agaaagtatt caaaaatcag cagatgcttt catgcaaaag catccaaaaa cgaaaatcaa
     1321 gattgaaaca ttttcttgga atgacttcta tactaaatgg actacaggtt tagcaaatgg
     1381 aaatgtgcca gatatcagta cagctcttcc taaccaagta atggaaatgg tcaactcaga
     1441 tgctttggtt ccgctaaatg attctatcaa gcgtattgga caagataaat ttaacgaaac
     1501 tgccttaaat gaagcaaaaa tcggagatga ttactactct gttcctcttt attcacatgc
     1561 acaagtcatg tgggttagaa cagatttgtt aaaagaacat aatattgagg ttcctaaaac
     1621 ttgggatcaa ctctatgaag cttctaaaaa attgaaagaa gctggagttt atggcttgtc
     1681 tgttccgttt ggaacaaatg acttaatggc aacacgtttc ttgaacttct acgtacgtag
     1741 tggtggagga agcctcttaa caaaagatct taaagcagac ttgacaagcc aacttgctca
     1801 agatggtatt aaatactggg ttaaattgta taaagaaatc tcacctcaag attctttgaa
     1861 ctttaatgtc cttcaacaag ctaccttgtt ctatcaagga aaaacagcat ttgactttaa
     1921 ctctggcttc catatcggag gaattaatgc caacagtcct caattgattg attcgattga
     1981 tgcttatcct attccaaaaa tcaaagagtc tgataaagac caaggaattg aaacctcaaa
     2041 cattccaatg gttgtttgga aaaattcaaa acatccagaa gttgctaaag cattcttaga
     2101 agcactttat aatgaagaag actacgttaa attccttgat tcaactccag taggtatgtt
     2161 gccaactatt aaggggatta gcgattctgc agcctataaa gaaaatgaaa ctcgtaagaa
     2221 gtttaaacat gctgaagaag taattactga agctgttaaa aaaggtactg ctattggtta
     2281 tgaaaatgga ccaagtgtac aagctggtat gttgactaac caacacatta ttgaacaaat
     2341 gttccaagat atcattacaa atggaacaga tcctatgaaa gcagcaaaag aagcagaaaa
     2401 acaattaaac gatttatttg aggctgttca gtagatgtaa aagactagaa aataggtggg
     2461 ctagtgagct gaaaagctct agcccaatct tgtaaaagaa gggagaagga gaatggttaa
     2521 agaacgtaat ttaactcgct ggatatttgt tttgccagct atgattatcg taggattact
     2581 ctttgtttat ccgtttttct cgagtatttt ttatagcttt accaataagc atttgattat
     2641 gcctaattat aaatttgttg gtttggctaa ctataaagct gtgctatcag atcccaactt
     2701 ctttaatgcg ttctttaatt caattaagtg gaccgttttc tcattagttg gtcaagtttt
     2761 agtagggttt gtattggctt tagctcttca cagagtacgc cacttcaaga aattatatag
     2821 gaccttattg attgttcctt gggcatttcc tacaatcgtt attgccttct cttggcagtg
     2881 gattctaaac ggggtttatg gctacttacc taatctaatc gtaaaattag gtttaatgga
     2941 acatacacct gcatttttga cagatagtac atgggcattc ctatgtttgg tgtttatcaa
     3001 catttggttt ggagcaccaa tgattatggt taatgtgctt tcagctttgc aaacagtacc
     3061 agaagaacaa tttgaggctg ctaagataga tggtgcttca agttggcagg tgttcaagtt
     3121 tatcgtcttt ccacatatta aagtggttgt aggacttcta gttgttttga gaactgtatg
     3181 gatctttaat aactttgaca ttatctacct cattactggt ggtggaccag ccaatgctac
     3241 aacgacgctt ccaatttttg cttacaacct gggctgggga actaaattgt tgggtcgtgc
     3301 ttcagcagtt acagtattgc tctttatctt cttggtggcg atttgcttta tctactttgc
     3361 tatcatcagt aagtgggaaa aggagggtag aaaataatga agaagaaatc cagtatttat
     3421 ttagatattc tctcacatgt acttttagtt ggtgcgacca tcgttgcaat tttcccattg
     3481 gtatggatta tcatatcttc tgtcaaaggg aaaggggaat taactcagta tccaacacga
     3541 ttttggcctg aacagtttac attagattat ttcactcatg ttatcaacga tttgcacttc
     3601 attgataaca ttcgaaacag tttaatcatt gccttggcta caaccctcat tgcgattatt
     3661 atttctgcta tggcagccta tggtattgtt cgattctttc ctaaattggg agcaatcatg
     3721 tcgagactac tcgtcattac ctacattttc ccaccaattt tgttagcaat tccctattca
     3781 attgccattg ctaaagttgg gttaacaaat agtttatttg gcttgatgat ggtttatcta
     3841 tcttttagtg ttccatatgc agtttggctc ttagttggat ttttccaaac agttccaatt
     3901 ggaattgaag aagcggctag aattgatggt gcaaataaat ttgttacgtt ttataaggtt
     3961 gtgctaccga ttgtagcacc aggtattgta gcaacagcta tttatacatt tatcaatgct
     4021 tggaatgaat tcctgtatgc cttgattttg attaacaata caggaaagat gacagtagca
     4081 gtagcccttc gttcacttaa tggttcagaa atactagact ggggagatat gatggcagcg
     4141 tctgttattg tagttcttcc atcaattatt ttcttctcta tcatccaaaa taagattgca
     4201 agtggattat cagaaggatc tgtgaagtag acgaaagaag gaaaaaaatg aataaaagag
     4261 gtctttattc aaaactagga atttctgttg taggcattag tcttttaatg ggagtcccca
     4321 ctttgattca tgcgaatgaa ttaaactatg gtcaactgtc catatctcct atttttcaag
     4381 gaggctcata tcaactgaac aataagagta tagatatcag ccctttgtta ttagataaat
     4441 tgtctggaga gagtcagaca gtagtaatga aatttaaagc agatgaacca aactctcttc
     4501 aagctttgtt tggcctatct aatagtaaag caggctttaa aaataattac ttttcaattt
     4561 tcatgagaga ttctggtgag ataggtgtag aaataagaga cgcccaaaag ggaataaatt
     4621 atttattttc tagaccagct tcattatggg gaaagcataa aggacaggca gttgaaaata
     4681 cactagtatt tgtatctgat tctaaagata aaacatacac aatgtatgtt aatggaatag
     4741 aagtgttctc tgaaacagtt gatacatttt tgccaatttc aaatataaat ggtatagata
     4801 aggcaacact aggagctgtt aatcgtgaag gtaaggaaca ttacctcgca aaaggaagta
     4861 ttgatgaaat cagtctattt aacaaagcaa ttagtgatca ggaagtttca aatattccct
     4921 tgtcaaatcc atttcagtta attttccaat caggagattc tactcaagct aactatttta
     4981 gaataccgac actatataca ttaagtagtg gaagagttct atcaagtatt gatgcacgtt
     5041 atggtgggac tcatgattct aaaagtaaga ttaatattgc cacttcttat agtgatgata
     5101 atgggaaaac gtggagtgag ccaatttttg ctatgaagtt taatgactat gaggagcagt
     5161 tagtttactg gccacgagat aataaattaa agaatagtca aattagtgga agtgcttcat
     5221 tcatagattc atccattgtt gaagataaaa aatctgggaa aacgatatta ctagctgatg
     5281 ttatgcctgc gggtattgga aataataatg caaataaagc cgactcaggt tttaaagaaa
     5341 taaatggtca ttattattta aaactaaaga agaatggaga taacgatttc cgttatacag
     5401 ttagagaaaa tggtgtcgtt tatgatgaaa caactaataa acctacaaat tatactataa
     5461 atgataagta tgaagttttg gagggaggaa agtctttaac agtcgaacaa tattcggttg
     5521 attttgatag tggctcttta agagaaaggc ataatggaaa acaggttcct atgaatgttt
     5581 tctacaaaga ttcgttattt aaagtgactc ctactaatta tatagcaatg acaactagtc
     5641 agaatagagg agagagttgg gaacaattta agttgttgcc tccgttctta ggagaaaaac
     5701 ataatggaac ttacttgtgt cctggacaag gtttagcatt aaaatcaagt aacagattga
     5761 tttttgcaac atatactagt ggagaactaa cctatctcat ttctgatgat agtggtcaaa
     5821 catggaagaa atcctcagct tcaattctgt ttaaaaatgc aacagcagaa gcacaaatgg
     5881 ttgaactgag agatggtgtg attagaacat tctttagaac cactacaggt aagatagctt
     5941 atatgactag tagagattct ggagaaacat ggtcggaagt ttcgtatatt gatggaatcc
     6001 aacaaacttc atatggcaca caagtatctg caattaaata ctctcaatta attgatggaa
     6061 aagaagcagt cattttgagt acaccaaatt ctagaagtgg ccgtaaggga ggtcaattag
     6121 ttgtcggttt agtcaataaa gaagatgata gtattgattg gaaataccac tatgatattg
     6181 atttgccttc gtatggttat gcctattctg cgattacaga attgccaaat catcacatag
     6241 gtgtactgtt tgaaaaatat gattcgtggt cgagaaatga attgcattta agcaatgtag
     6301 ttcagtatat agatttggaa attaatgatt taacaaaata aaggagaaaa acatggttaa
     6361 atacggtgtt gttggagcag ggtattttgg agctgaattg gctcgctata tgcaaaagaa
     6421 tgatggagca gagattactc ttctctatga tccagataat gcagaggcga ttgcagaaga
     6481 attgggagca aaagtagcaa gttccttaga tgagttggtt tctagcgatg aagtagattg
     6541 tgttatcgtc gcaactccaa ataatcttca taaggaaccg gttattaagg ctgcacagca
     6601 tggtaaaaat gttttctgtg aaaaaccaat tgcgctttct tatcaagatt gtcgcgagat
     6661 ggtagatgcg tgtaaagaaa acaatgtaac ctttatggca ggacatatta tgaatttctt
     6721 taatggtgtt catcatgcaa aagaactcat taatcaagga gttatcggag acgttctata
     6781 ttgtcataca gctcgtaatg gttgggaaga acaacaaccg tcagtatcat ggaaaaaaat
     6841 tcgtgaaaaa tcaggtggtc acttgtatca ccacatccat gaattggatt gcgttcaatt
     6901 ccttatgggg ggcatgcctg aaactgtaac catgacaggt ggaaatgtgg cccatgaagg
     6961 tgaacatttc ggtgatgaag atgatatgat ttttgtcaat atggaatttt ctaataagcg
     7021 ttttgccttg ttagaatggg gttcagctta tcgttggggt gaacattatg tcttaatcca
     7081 aggaagcaaa ggtgccatcc gcttagactt attcaactgt aaaggaactc ttaagctaga
     7141 tgggcaagaa agctatttct tgattcacga atcgcaagaa gaagatgatg atcggactcg
     7201 tatctatcat agtacagaga tggatggagc aattgcttat ggtaaaccag gtaaacgtac
     7261 tccattatgg ctatcatctg tcattgataa agaaatgcgc tatctgcatg agattatgga
     7321 aggagctcca gtatcagaag aatttgcaaa acttttgaca ggtgaagctg ccctagaagc
     7381 aattgctact gcagatgctt gtacccagtc tatgtttgaa gatcgcaaag taaaattgtc
     7441 agaaattgta aaataaattt tggtattctc ctattatagg tcgacttgct cctctgaaag
     7501 tacttttaga ggagctgttt gactttgcta gtttttgaaa ctgaaatcta ttatactaca
     7561 aactattgaa agcgttttaa ttttaaggta taataatctc atagaaataa agaaaaggag
     7621 gaaagaggat gccacagatt agcaaagaag ccttgattga gcaaatcaaa gatggaatca
     7681 tcgtttcttg tcaggctctt cctcatgaac cgctttatac agaagcggga ggggtgattc
     7741 ccttgctggt caaagcggct gagcaaggtg gagcagtcgg tatccgagca aacagtgttc
     7801 gcgatatcaa ggaaattaag gaagtcacta aacttccaat cattgggatt atcaaacgtg
     7861 attatccacc tcaggaaccc ttcatcacgg ctactatgaa agaagttgat gaattggcag
     7921 aactggacat cgaggtgatt gctctggatt gtaccaagcg tgaacgctac gatggtttgg
     7981 aaattcaaga gttcattcgt caggttaagg agaaatatcc taatcagctt ttgatggctg
     8041 atactagtat cttcgaagaa gggctagcag ctgtagaagc aggaattgac tttgtcggaa
     8101 caaccttatc aggctacaca tcctacagtc caaaagtaga cggtccagat tttgaattga
     8161 ttaagaaact ctgtgatgct ggtgtagatg tcattgcaga aggaaaaatt catacaccag
     8221 aacaagccaa acaaatcctt gaatatggag tgcgaggcat cgttgttggt ggcgccatta
     8281 ctagaccaaa agagattaca gaacgcttcg ttgctagtct taaataagat gtgaggggga
     8341 gttttatgtt taaagtttta caaaaagttg gaaaagcttt tatgttacct atagctatac
     8401 ttcctgcagc aggtctactt ttggggattg gtggtgcact ttcaaaccca accacgatag
     8461 caacttatcc aatactagac aatagtattt ttcaatcgat attccaagta atgagctctg
     8521 caggagaggt tgtattcagt aatttgtcac tacttctctg tgtgggatta tgtattggct
     8581 tagcgaaacg agataaagga accgctgcgt tagcaggagt aactggttac ttagttatga
     8641 ctgcaacgat caaagctttg gtaaaacttt ttatggcaga aggaactgca attgatactg
     8701 gagttattgg agcattagct gtcggaatag ttgccgtata tttgcacaac cgatataaca
     8761 atattcaatt accttccgct ttaggattct ttggaggttc acgcttcgtt cctattgtta
     8821 catcgttctc ttctatcttg attggctttg tcttctttgt tatttggcca cctttccaac
     8881 aacttcttgt ttctacaggt ggatatattt ctcaggcggg tccaattgga acttttctat
     8941 atggattttt aatgagactt tctggagcag taggcttaca tcatataatt taccctatgt
     9001 tttggtatac tgaacttggt ggtgttgaaa ctattgcagg acaaacagtg gttggagctc
     9061 aaaaaatatt ttttgctcaa ttagccgatc cggcccattc tggattattt acagaaggaa
     9121 caaggttttt tgcaggtcgt ttctcaacaa tgatgttcgg tttaccggct gcctgtttag
     9181 cgatgtacca tagtgttcct aaaaatcgtc gtaaaaaata cgcgggtttg ttttttggag
     9241 ttgctttaac atcttttatt accggtatta cagaaccaat tgaatttatg tttctattcg
     9301 tcagtccggt tctatatgtt gttcacgcat tccttgatgg tgttagcttc tttattgcag
     9361 acgtcttaaa tatttcaata ggaaacacat tttcaggagg tgtaatcgat ttcactttat
     9421 ttggaatttt gcaggggaac gctaagacga attgggttct tcagattcca attggactta
     9481 tttggagtgt tttgtattat attattttta gatggttcat tactcaattc aatgttctaa
     9541 cgccaggacg aggagaagaa gtagattcta aagaaatttc tgaatccgca gattcaactt
     9601 caaatactgc agattattta aaacaggata gcctacaaat tatcagagcc ttgggtggat
     9661 caaataatat agaagatgta gatgcttgtg tgacacgttt acgtgtagct gtaaaagaag
     9721 ttaatcaagt tgataaagca cttttaaaac aaattggtgc agttgatgtc ttagaagtga
     9781 agggtggcat tcaagcaatc tatggagcaa aagcaatctt atataaaaat agtattaatg
     9841 aaattttagg tgtagatgat taagtactta ctgacttaat aaaaaacaga ggagagtgat
     9901 ggatgagtag gatgaaatga aatcgcatac aagaaataaa gaactcatta tccaagttgg
     9961 atacgcttat tacataggag aatacaaatg aaatttagaa aattagcttg tacagtactt
    10021 gcgggtgctg cggttcttgg tcttgctgct tgtggcaatt ctggcggaag taaagatgct
    10081 gccaaatcag gtggtgacgg tgccaaaaca gaaatcactt ggtgggcatt cccagtattt
    10141 acccaagaaa aaactggtga cggtgttgga acttatgaaa aatcaatcat cgaagcgttt
    10201 gaaaaagcaa acccagatat aaaagtgaaa ttggaaacca tcgacttcaa gtcaggtcct
    10261 gaaaaaatca caacagccat cgaagcagga acagctccag acgtactctt tgatgcacca
    10321 ggacgtatca tccaatacgg taaaaacggt aaattggctg agttgaatga cctcttcaca
    10381 gatgaatttg ttaaagatgt caacaatgaa aacatcgtac aagcaagtaa agctggagac
    10441 aaggcttata tgtatccgat tagttctgcc ccattctaca tggcaatgaa caagaaaatg
    10501 ttagaagatg ctggagtagc aaaccttgta aaagaaggtt ggacaactga tgattttgaa
    10561 aaagtattga aagcacttaa agacaagggt tacacaccag gttcattgtt cagttctggt
    10621 caagggggag accaaggaac acgtgccttt atctctaacc tttatagcgg ttctgtaaca
    10681 gatgaaaaag ttagcaaata tacaactgat gatcctaaat tcgtcaaagg tcttgaaaaa
    10741 gcaactagct ggattaaaga caatttgatc aataatggtt cacaatttga cggtggggca
    10801 gatatccaaa actttgccaa tggtcaaaca tcttacacaa tcctttgggc accagctcaa
    10861 aatggtatcc aagctaaact tttagaagca agtaaggtag aagtggtaga agtaccattc
    10921 ccatcagacg aaggtaagcc agctcttgaa taccttgtaa acgggtttgc agtattcaac
    10981 aataaagacg acaagaaagt cgctgcatct aagaaattca tccagtttat cgcagatgac
    11041 aaggagtggg gacctaaaga cgtagttcgt acaggtgctt tcccagtccg tacttcattt
    11101 ggaaaacttt atgaagacaa acgcatggaa acaatcagcg gctggactca atactactca
    11161 ccatactaca acactattga tggatttgct gaaatgagaa cactttggtt cccaatgttg
    11221 caatctgtat caaatggtga cgaaaaacca gcagatgctt tgaaagcctt cactgaaaaa
    11281 gcgaacgaaa caatcaaaaa agctatgaaa caatagtcct tagttattct ataaaaagta
    11341 gttttttaaa gaacctaaga gtgtataccc ccttttccct ctacacagat agtgtaagaa
    11401 aagggggctt ttgtttaaaa tgtaagaaac tgtcacgaaa ttaaaatgaa gttcttacat
    11461 aagcgaatca taaaaaattt cattttgatt ttaaaacagt tcaagaaagt caaaaaatta
    11521 ttctatttga aagagaggtg ccgactgtga aagtcaataa aatccgtatg cgggaaacag
    11581 tgatttccta cgctttccta gcaccagtat tattcttctt tgtcatcttt gtgttggctc
    11641 cgatggtgat gggcttcatt acaagtttct ttaactactc aatgactaaa tttgagtttg
    11701 taggcttgga taactatatc cgtatgttta aagatcctgt ctttacaaaa tctctgatta
    11761 acacagttat tttggttatt ggatctgtac cagttgttgt tctattctca ctctttgtag
    11821 catctcagac ctatcatcaa aatgtcattg ccagatcctt ctaccgtttc gtcttcttcc
    11881 ttcctgttgt aacgggtagt gttgccgtga cagttgtttg gaaatggatt tatgacccac
    11941 tatcagggat tctaaacttt gtccttaagt ccagccacat catcagccaa aacatttctt
    12001 ggttgggaga taaaaactgg gcattgatgg cgattatgat tattctcttg accacttcag
    12061 ttggtcagcc catcatcctt tatatcgctg ccatggggaa tattgacaat tcactggttg
    12121 aagcggcgcg tgttgatggt gcaactgagt ttcaagtttt ttggaagatt aaatggccaa
    12181 gccttcttcc aacaactctt tatattgcaa tcatcacaac aattaactca ttccagtgtt
    12241 ttgccttgat tcagcttttg acatctggtg gtccaaacta ctcaacaagt accttgatgt
    12301 actaccttta cgaaaaagcc ttccaattga cagaatacgg ctatgccaac acaattggtg
    12361 tcttcttggc agtcatgatt gctatcgtaa gctttgttca atttaaagta cttggaaacg
    12421 acgtagaata ctaaagaaag gagacagcta tgcaatctac agaaaaaaaa ccattaacag
    12481 cctttactgt tatttcaaca atcattttgc tcttgttgac tgtgctgttc atctttccat
    12541 tctactggat tttgacaggg gcattcaaat cacaacctga tacaattgtt attcctcctc
    12601 agtggttccc taaaatgcca accatggaaa acttccaaca actcatggtg cagaaccctg
    12661 ccttgcaatg gatgtggaac tcagtattta tctcattggt aaccatgttc ttagtctgtg
    12721 caacctcatc tctagcaggt tatgtattgg ctaaaaaacg tttctatggt caacgcattc
    12781 tatttgctat ctttatcgct gctatggcgc ttccaaaaca agttgtcctt gtaccattgg
    12841 tacgtatcgt caacttcatg ggaatccacg atactctctg ggcagttatc ttgcctttga
    12901 ttggatggcc attcggtgtc ttcctcatga aacagttcag tgaaaatatc cctacagagt
    12961 tgcttgaatc agctaaaatc gacggttgtg gtgagattcg taccttctgg agtgtagcct
    13021 tcccgattgt gaaaccaggg tttgcagccc ttgcaatctt taccttcatc aatacttgga
    13081 atgactactt catgcaattg gtaatgttga cttcacgtaa caatttgacc atctcacttg
    13141 gggttgcgac catgcaggct gaaatggcaa ccaactatgg tttgattatg gcaggagctg
    13201 cccttgctgc tgttccaatc gtcacagtct tcctagtctt ccaaaaatcc ttcacacagg
    13261 gtattactat gggagcggtc aaaggataat actctgagaa aatctcttca aactacgtca
    13321 gcgtcacctt gccatactta agtattgcct gcggttagct tcctagtttg ttcttcaatt
    13381 ttcattgagt ataggaaaat caatctatca agatacagaa gtatatttta tagatttaga
    13441 gaatatagag gttataagtg tctacaaaat ggagggtatg cagttacttt atgaagtttt
    13501 gtcagacact tataaactta agaatggttt tagttaacta tcagaaacga aggaaagagt
    13561 atgatttttg acgatttgaa aaacatcacc ttttacaaag ggattcatcc caatttagac
    13621 aaggctatcg actatctcta ccaacatcgt aaagattcat tcgaattagg aaagtatgag
    13681 attgatggag ataaagtctt tctagttgtt caggaaaatg tcctcaatca agttgagaat
    13741 aatcgatttg aacaccataa gaactatgca gatttgcatt tgctgataga agggcatgaa
    13801 tattcgagct acggttcacg tatcaaagac gaggcagtag cattcgacga agcgagtgac
    13861 attggctttg ttcattgtca tgaacactac ccactcttgt tgggttatca caattttgcg
    13921 attttcttcc caggtgagcc acatcagcca aatggttatg caggcatgga agaaaaggtt
    13981 cgaaaatatc tctttaaaat tttgattgat taaaaatagg atgaattgtt tttttgtaaa
    14041 gctttgataa tactctacca tgaaattgat ctttgtgagg tagagaaatg agaataaaat
    14101 atttaaaaat tggtatcttc taagtatgct gcaagagcta gtttcttaga tggacagggg
    14161 attacagttg atgagatggc ttggataatt aggggcattg tgactgcatt gattggtaga
    14221 tacataaaat taggtactta tgcagttaag tatggtatta gtatggcgcg ctcgatctta
    14281 agtagggcag ctgcaactgc ggcagcaaga gtaggattat tgactaagat ttttggatgg
    14341 attttacgaa tagctgtgct cgcagctgat gtatatggta attttgccaa caatattgct
    14401 gtagcttggg atgcacatga taaaatccct aacaatggtc gtataaactt ttaaaatgcg
    14461 agaatgaaag cactttgtat ttttttattg tatatgttag cttggacagt gcttgcaatg
    14521 ataatccgtg gagggctaga tggatttgat gaacacactt ggcgtaatat tttagctagt
    14581 gcgttgtcct cgctatacat acattatata tccatagata gaaaaagaaa aaagtccaaa
    14641 agaaaaaaga aaaaatagat ttgttcatgg tagggactta tgaaagcttt actgacaaaa
    14701 aagaaaacag tttacaaaga aaaatgatgg aggagcaaac atggcacaaa aaggagtaag
    14761 ccttatcaag gcagcatttg atacagataa ctttctcatg cgttttagtg agaaagtctt
    14821 ggacatcgtg acagccaatc ttctttttgt cgtctcttgt ttgcccatcg tgacgattgg
    14881 agtggctaaa atcagcctct acgagaccat gttcgaagtt aagaagagca gacgggtgcc
    14941 tgtttttaaa atctatctaa gatctttcaa gcaaaatctg aaactaggtc ttcagctggg
    15001 tttaatggag ttaggaattg tgtttcttac cctttcagat ctctatcttt tctggggtca
    15061 aacagctctg cccttccaat tgctgaaagc catttgtcta ggcattctga ttttccttac
    15121 gattgtgatg ttggctagct atccaatcgc ggcacgttat gatttaactt ggaaagaagt
    15181 gttacaaaaa gggcttatct tggctagttt taactttcct tggttcttcc tcatgttagc
    15241 cattcttgtc ctcattgtga tggttcttta tctgtccgcc ttcagtctac tcttaggtgg
    15301 ctcagtcttc ctactttttg ggtttggact attggtcttt atccagactg gattgatgga
    15361 gaaaattttc gcaaaatacc aataggagcc ttatttctga aactactttc aaaggctcca
    15421 aacgctattc tataagcgag aaactaaaat ctaagtgtct ataagatatt gaaagccttt
    15481 tcggtaaagt gtatactaaa gaagtaaaga aaaatctgct ttagcagaaa ataaaaataa
    15541 taggagaaaa tctatgtcag atttgaaaaa atacgaaggt gtcattccag ccttctacgc
    15601 atgttatgat gatcaaggag aagtaagccc agaacgtacg cgtgccttgg ttcaatactt
    15661 cattgataaa ggtgttcaag gtctttatgt caatggttct tctggtgaat gtatctacca
    15721 aagcgttgaa gatcgcaagt tgattttgga agaagtcatg gcggtagcca aagataaatt
    15781 gaccattatt gcccatgttg cttgcaataa tactaaagat agtatggaac ttgctcgcca
    15841 tgctgaaagc ttgggagtag atgctattgc aacgattcca ccaatttatt tccgcttgcc
    15901 agaatactca gttgccaaat actggaacga tatcagttct gcagctccaa acacagacta
    15961 cgtgatttac aacattcctc aattggcagg ggttgctttg actccaagcc tttacacaga
    16021 aatgttgaaa aatcctcgtg ttattggtgt gaagaactct tctatgccag ttcaagatat
    16081 ccaaaccttt gtcagccttg gtggagaaga ccatatcgtc tttaatggtc ctgatgagca
    16141 gttcctagga ggacgcctca tgggggctag ggctggtatc ggtggtactt atggtgctat
    16201 gccagaactc ttcttgaaac tcaatcagtt gattgcggat aaggacctag aaacagcgcg
    16261 tgaattgcag tatgctatca acgcaatcat tggtaaactc acttctgctc atggaaatat
    16321 gtacggtgtc atcaaagaag tcttgaaaat caatgaaggc ttgaatattg gatctgttcg
    16381 ttcaccattg acaccagtga ctgaagaaga tcgtccagtt gtagaagcgg ctgctgcctt
    16441 gattcgtgaa accaaggagc gcttcctcta atctaaaagg aggtatttat gacatattac
    16501 gttgcaattg atatcggtgg aaccaacatc aagtatggtt tggttgatca agaggggcaa
    16561 cttcttgaat cgcatgaaat gccaactgag gcgcataagg gtggacctca tatcttacaa
    16621 aagaccaaag atatcgtagc tagttattta gaaaaaggcc cagtagcagg tgttgccata
    16681 tcttctgctg ggatggtgga tccggataag ggtgagattt tctatgctgg gccgcaaatc
    16741 cctaactacg caggcaccca gttcaaaaag gaaatcgaag aaagctttac tattccttgt
    16801 gagattgaaa atgatgtcaa ctgtgcaggt cttgctgagg cagtatctgg ttcaggcaag
    16861 ggagcaagtg tgacactttg cttgaccatt ggaacaggta tcggtggttg cttgattatg
    16921 gataggaaag tcttccatgg ttttagcaat tcagcctgtg aagtcgggta tatgcatatg
    16981 caggatggag cttttcagga tctcgcttct acaacagctt tagtgaaata tgtagctgaa
    17041 gcccatggag aagatgttga tcagtggaat ggccgacgta tctttaagga agctactgaa
    17101 ggaaacaaaa tctgcatgga aggcattgac cgtatggttg actatctagg aaaaggtctg
    17161 gcaaatattt gctacgttgc caatccagaa gtggttattc ttggtggtgg tatcatgggg
    17221 caagaggcta tcctcaaacc taagatccgc acagccttga aagaggcttt ggtaccaagt
    17281 ttagcagaaa aaacacgatt agaatttgcc catcaccaaa atacagcagg gatgttgggt
    17341 gcatattatc attttaagac aaaacaatcc tagtttggct cagccaaact aggattttct
    17401 tacacgtttt tgtctacgat agccgttgag ttttttattt tcccagtagc tattaaagat
    17461 tttttccttg ctttcgcgat tgatttccaa aaagtaggca taaatcaaat cgataaagaa
    17521 gagcatagga agttgagcgg atattcgttg gatataggag ggttggctgt gggtggctac
    17581 aagaacagtc tctgtatagg tctggctatc tttattggga acacttgtaa agagtacagt
    17641 ctttgccccc atctccttag catctaatag actatctaaa atagaaggag ttgagcctga
    17701 aagtgagaag cccagtacta gacaattttc atccatgatg ctggttgtcc aggcaaagcc
    17761 gtcttggtct gtcaaagctt cgcagaccac acctagtcgc ataaaacgta atttcatttc
    17821 acgggcgacg aggccagaac tccctgttcc aaagaagtag atacgctcag catcttcgat
    17881 tagctgggca attcgttcta gttggatttc gtcaatcaag tcttgtgttt gttccctcat
    17941 attgctataa cttctgagga ctcgtttggt cagtggactg tgcttggaga cttggttggc
    18001 ttgattttct gcctgatgtt ggtattggaa aataaattct cggtagccag taaagccaca
    18061 ctttttagca aagcgggtca aagcagcttg agaaatatgt aatttttggg tgacttgttg
    18121 agaagataaa tcatctgtaa tcgtttcagc ttgcaaaaaa tagcgagcga tttcttgttc
    18181 taggtctgtc atttcttcaa aatgtgaatc aatgatagtt gcgatatctg gtttgtccat
    18241 agggaaagct cctttacatg agtcatactg gaagactaga tcagagaata gtcacacttc
    18301 a
//
LOCUS       DAVGJL010000025        18176 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.025, whole genome shotgun sequence.
ACCESSION   DAVGJL010000025 DAVGJL010000000
VERSION     DAVGJL010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18176
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.025"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..115
                     /gene="blpC"
                     /locus_tag="VVE42_000764"
     CDS             <1..115
                     /gene="blpC"
                     /locus_tag="VVE42_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HEU3072984.1"
                     /translation="NLTSFQELTTTELNQITGGGLWEDLLYNINRYAHYIT"
     gene            complement(186..1526)
                     /locus_tag="VVE42_000765"
     CDS             complement(186..1526)
                     /locus_tag="VVE42_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU3072985.1"
                     /translation="MNIAWILLYTLVTNGLEIVIFFKVDGIDLTFERIFKAFLLKILL
                     AFVFVMISYIVGNVYLSYFMEPLYGIGLSFLLLRGLPKKLLFFYGLFPMILVNLFYRG
                     VSYFVLPFLGQGQVYDGYSFTGLCIIIFNFFISLAFLKWLDYDFTSLRKEILDKAFQK
                     SLTQINWIMGGYYLVMESLSFFEYEQSIQSKTVRHLILVFYLLFFMGVIKKLDTYLKE
                     KLYERLEQEQALRYRDMERYSRHIEELYKEVRSFRHDYTNLLTSLRLGIEEEDMEQIK
                     EVYGSVLKDSSQKLQNNKYDLGRLVNIRDKALKSLLAGKFLKARDKNIVFNVEVPEEI
                     QVEGMSLLDFLTIVSILCDNAIEASVEASQPHVSIAFLKNGAQETFIIENSIKEEGID
                     ISEIFSFGASSKGEERGVGLYTVMKIVESHPNTSLNTTCQNQVFRQVLTVIHTE"
     gene            complement(1540..2277)
                     /locus_tag="VVE42_000766"
     CDS             complement(1540..2277)
                     /locus_tag="VVE42_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU3072986.1"
                     /translation="MRIFVLEDDFSQQTRIETTIEKLLKAHHIIPSSFEVFGKPDQLL
                     AEVHEKGAHQLFFLDIEIRNEEMKGLEVARKIRDRDPYALIVFVTTHSEFMPLSFRYQ
                     VSALDYIDKALSAEEFESRIETALLYANSQDSKSLAEDCFYFKSKFAQFQYPFKEVYY
                     LETSPRAHRVILYTKTDRLEFTASLEEVFKQEPRLLQCHRSFLINPANVVHLDKKEKL
                     LFFPNGGSCLIARYKVREVSEAINKLH"
     gene            complement(2282..2620)
                     /locus_tag="VVE42_000767"
     CDS             complement(2282..2620)
                     /locus_tag="VVE42_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU3072987.1"
                     /translation="MKHMIIQTQKTVYKVNIDDIYYIQTHPTKAHTVQIVTEEASFNM
                     LKNLSNLENQCGETLMRCHRNCLVNLDKLKSIDFQERILFLGEEGQYAVKYARRRYRE
                     IRQKWLKEGE"
     gene            2896..3225
                     /locus_tag="VVE42_000768"
     CDS             2896..3225
                     /locus_tag="VVE42_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEU3072988.1"
                     /translation="MTDTDPIKRAHTLITDLNKAYQACKQASADDLRFQEQLNSILGF
                     LAKAETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTKLIL
                     NENQRAN"
     gene            complement(3393..4442)
                     /locus_tag="VVE42_000769"
     CDS             complement(3393..4442)
                     /locus_tag="VVE42_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU3072989.1"
                     /translation="MKDLFLKRKQAFRKECLGYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQDFPENHWPILLFVGITSVLLLLWGGIATYMEAPDKLFLLVGEEEIKLHLKRQTG
                     ISLVFWLFVQTLFLLLFAPLFLAMGYGLPVFLLYVLLLGVGKYFHFCQKASKFFTETG
                     LDWDYVISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKAVQKVPGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSVLAQVFIEQAWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQEVVRGLTSFVLLVELVVGLITFQEKLALLALLGAGLVL
                     QVLYLPYQVKRQMQD"
     gene            complement(4439..5170)
                     /locus_tag="VVE42_000770"
     CDS             complement(4439..5170)
                     /locus_tag="VVE42_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3072990.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLAPYSGSININGLTMQGDATSYRKQIGYIPETPSLYEELTLREHIETVAMAY
                     GIEQKVAFERVEPLLKMFRLDQKLDWFPVHFSKGMKQKVMIICAFVVDPSLFIVDEPF
                     LGLDPLAIADLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRAKGNLLQ
                     LREAFNMPEASLNDIYLALTKEEEL"
     gene            5238..5648
                     /locus_tag="VVE42_000771"
     CDS             5238..5648
                     /locus_tag="VVE42_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEU3072991.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPK
                     EHYRNLLEMDATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA"
     gene            5658..5945
                     /locus_tag="VVE42_000772"
     CDS             5658..5945
                     /locus_tag="VVE42_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3072992.1"
                     /translation="MKLSNLLLFAGAAAGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIIDQQKELIKEYQEDLTYKFKVLEKDIQTRLAMIKEMQGTEDK"
     gene            6107..6391
                     /locus_tag="VVE42_000773"
     CDS             6107..6391
                     /locus_tag="VVE42_000773"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3072993.1"
                     /translation="MHHNLGNSYSIKIKKQTRKLGTDCSKHRFKVVDRTDEVNSKHCF
                     EVVDRTDEVSSKHCFEVVDRTDEVSNHTHDKPTLTWFEEIFEEYHSPFHN"
     gene            complement(6725..7861)
                     /gene="dnaJ"
                     /locus_tag="VVE42_000774"
     CDS             complement(6725..7861)
                     /gene="dnaJ"
                     /locus_tag="VVE42_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEU3072994.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGAGGFGGFNGAGGFGGFEDIFSSF
                     FGGGGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGT
                     SPVTCGRCHGSGVINVDTQTPLGMMRRQVTCDVCHGRGKEIKYPCTTCHGTGHEKQAH
                     SVHVKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYAVVSVEDSDKFEREGTTIFYNLN
                     LNFVQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRGKGAPSLRGGAVGDQYVTVN
                     VVTPTGLNDRQKVALKEFAAAGDLKVNPKKKGFFDHIKDAFDGE"
     gene            complement(8134..8490)
                     /locus_tag="VVE42_000775"
     CDS             complement(8134..8490)
                     /locus_tag="VVE42_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine-N7)-methyltransferase"
                     /protein_id="HEU3072995.1"
                     /translation="MSVLDDEYLKNTRKVYNDFCNQADNYEAAKGFIDNIPVECLTIY
                     REIILAEHDSCVKNDEAVRNFVTSVLLSAFVSAMVSAMISLEIQTYKFVIPFIIGMIW
                     TVVVFLMINWNYIGKY"
     gene            complement(8492..10315)
                     /gene="dnaK"
                     /locus_tag="VVE42_000776"
     CDS             complement(8492..10315)
                     /gene="dnaK"
                     /locus_tag="VVE42_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEU3072996.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKAKLEALNEKAQ
                     GLAVKLYEQAAAAQQAQEGAEGAQATGNAGDDVVDGEFTEK"
     gene            complement(10795..11319)
                     /gene="grpE"
                     /locus_tag="VVE42_000777"
     CDS             complement(10795..11319)
                     /gene="grpE"
                     /locus_tag="VVE42_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEU3072997.1"
                     /translation="MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LGMVQESLIHALKEEGIEEIAVDGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            complement(11346..12380)
                     /gene="hrcA"
                     /locus_tag="VVE42_000778"
     CDS             complement(11346..12380)
                     /gene="hrcA"
                     /locus_tag="VVE42_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEU3072998.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKALQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFDRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTDLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKSIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVHLLNFANLAAYQFFDQPQKVALEIREGLREEQMQNVRVADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQVNVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            complement(12544..13053)
                     /locus_tag="VVE42_000779"
     CDS             complement(12544..13053)
                     /locus_tag="VVE42_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HEU3072999.1"
                     /translation="MNSRVEFRIFTIVDLDKEEEYLHEMHLKGWRYRTSRFGLFYFDQ
                     CQPDDVIYRIYDSRFLKKYKYELQDFRDRGWELIGAGSCSILRKSSSDLLPEDQVYMS
                     KGLKWEVMRSRLRSCTATFLGGLVVCTSLFREDLSMSFFLIFVLYAFLISYLIHGYFR
                     LKRKYRVDE"
     gene            13661..15994
                     /locus_tag="VVE42_000780"
     CDS             13661..15994
                     /locus_tag="VVE42_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEU3073000.1"
                     /translation="MVEFIKSKKEMSEEDIKANFITPAIVSKGWKNGEHIAYEEYFTD
                     GRIEVRGDKARRKEGKKSDYSLYYQFGTRIAIVEAKDNKHSVRAGLQQVIEYGEILDV
                     PFVYSSNGDGFIEHDRITREERELELDEFPTREELFSRMTKEKGLTYEITEAISTPYY
                     TDAFSMKTPRYYQQIAINRTIETVARGQKRVMFVMATGTGKTFMAFQIIHRLRKAGLA
                     KRVLFLADRNILVDQTMAEDFRPFEKVMTKITPKLLTAPEKLNSFEIYLGLYQQLTGE
                     DGTETHYQKFDKDFFDLIVIDEAHRGSAKEDSNWRKVIDYFSSATQIGMTATPKETKN
                     ASNTEYFGEPIYTYSLKQGIEDGFLAPYRVMRVNLDVDVDGYRPETGKVDTNGQLIED
                     RYYGRKDFDKTIVIDDRTQRVAKFVSDYMKQSNARFDKTIVFCVDIDHAERMRAALVK
                     ENLDLVQEDYRYVMQVTGDNAEGKAQLDNFMDVNSNFPAIVTTSKLLTTGVNAKTCRL
                     IVLDSNIQSMTEFKQIIGRGTRLYPQKGKEFFTIIDFRNVTNLFADPDFDGEPVKVLE
                     TGAKTVSGSTPGFVDEEGDPVEKYIVTDKQVTILNSTIQVLDENGKLITESLTDYTRK
                     NILGSYATLNDFITVWHTADKKKLILDELYKKGVYLDAIRESEGISEQEIDDFDLLLK
                     LAYGQKELTKTERINKLKQSGYLYKYSEEARAVLEILLNKYMDKGIGELESIETLKLP
                     EFQIYGGTFKIINTYFGDKKRYLQAIKELEQELFTVA"
     gene            16007..17470
                     /locus_tag="VVE42_000781"
     CDS             16007..17470
                     /locus_tag="VVE42_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEU3073001.1"
                     /translation="MSITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREM
                     VWELEEDEYESIIPEELKWRNWAHAQNGERVLTGDELLDFVNNKLFKELKELEITSIM
                     PIRKTIVKSAFEDANNYMKNGVLLRQVINVIDEVDFNSPEDRHSFNDIYEKILKDIQN
                     AGNSGEFYTPRAATDFIAEVLDPKLGESMADLACGTGGFLTSTLNRLSSQRKTSEDTK
                     KYNTAVFGIEKKAFPHLLAVTNLFLHEIDDPKIVHGNTLEKNVREYTDDEKFDIIMMN
                     PPFGGSELETIKNNFPTELRSSETADLFMAVIMYRLKENGRVGVILPDGFLFGEGVKT
                     RLKQKLVDEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEETWFYRLDMPDGYKN
                     FSKTKPMKSEHFNPVRDWWENREEILEGKFYKSKSFTPSELAELNYNLDQCGFPKEEE
                     EILNPFELIQNYQAERATLNHKIDNVLADILQLLEDK"
     gene            17470..17743
                     /locus_tag="VVE42_000782"
                     /pseudo
     CDS             17470..17743
                     /locus_tag="VVE42_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001834076.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT         5736 a         3685 c         3452 g         5303 t
ORIGIN      
        1 aaacctaact tcatttcaag aactaacaac taccgaactc aaccaaatta caggtggagg
       61 attgtgggaa gatttattat ataacattaa tagatatgct cattacatca cataagaact
      121 tcatcatcca atacaactat aaaaaaataa gaccgagaaa caagtactct cggtcttatt
      181 tttcatcatt ctgtatgtat cacagtaagt acctgacgaa agacttgatt ttgacaggtg
      241 gtatttagac tggtattagg atggctttcc acaatcttca tgacggtata gagaccaact
      301 cctctctcct cccctttaga actggctcca aaggagaaga tttcagaaat atcgatgccc
      361 tcttctttga tggagttttc gatgataaag gtctcctgtg ctccattttt taaaaaggcg
      421 attgaaacat gaggttgact agcttccaca ctggcttcaa tagcattgtc acaaaggata
      481 gacacaatgg ttagaaaatc aagtagactc atcccctcga cctgaatctc ctcaggaact
      541 tcgacattaa agacaatgtt cttatctctg gcttttaaaa atttccctgc tagaagactt
      601 ttgagggctt tatcacgaat atttaccaat ctgcccaggt catatttatt gttctgcaat
      661 ttctgactgg aatcctttaa gacggagcca tagacctctt ttatctgctc catatcctcc
      721 tcttcaatgc ccagacgtag gctagtcaag aggttggtat aatcatgacg aaagctccgt
      781 acttccttgt aaagctcctc tatatgccga ctatagcgtt ccatatctct atagcgcagg
      841 gcctgctctt gttccaatct ctcatagagt ttttccttca aataggtatc caatttcttg
      901 ataaccccca taaaaaagag taggtaaaag actaggatga gatggcgaac agtctttgat
      961 tgaatacttt gttcatattc aaaaaaagac agactttcca tgactagata gtagccaccc
     1021 attatccagt taatctgagt aagagacttt tgaaaggctt tatcgagaat ctcctttctc
     1081 aagctagtaa aatcgtagtc caaccatttc aaaaaagcta gagaaatgaa gaaattgaaa
     1141 attattatac ataacccagt aaatgagtag ccatcatata cttgcccttg tcccaaaaat
     1201 ggaagcacaa aataggagac tcctctataa aagagattca ccaatatcat tggaaagaga
     1261 ccataaaaga aaaggagttt tttaggaagc cctctcaata ataagaaaga taagcctatg
     1321 ccgtacaagg gttccataaa ataagatagg taaacatttc ctactatata gctaatcatc
     1381 acaaaaacaa aggccaacag tatcttcaaa agaaaggcct taaaaatcct ctcgaaagta
     1441 agatcaattc catccacctt aaagaagatg acaatttcta gtccattagt aacaagtgta
     1501 tacaacaata tccaagcaat gttcataaat tctcctagct cagtgtaatt tattgatggc
     1561 ctcagacact tccctgacct tataacgggc gattagacaa cttccaccat tgggaaagaa
     1621 aaggagtttt tctttcttat ccaaatgcac cacatttgca ggattgatga gaaaagagcg
     1681 gtggcactgc aagagacggg gctcctgctt gaaaacctcc tctaaactcg ctgtaaattc
     1741 cagcctgtct gtcttggtat agagaataac acgatgggct ctgggcgacg tttcgagata
     1801 gtaaacctct ttaaaaggat actgaaattg ggcaaatttt gatttaaagt aaaagcaatc
     1861 ttccgccaga cttttactat cttgactatt ggcatagagg agggctgtct cgatccgaga
     1921 ttcaaactcc tctgctgaca aggccttatc aatgtagtcc aaagcagaca cttggtagcg
     1981 aaaagacagg ggcataaact ccgagtgagt cgtcacaaag acaatcaggg cataaggatc
     2041 ccgatcccga atctttctag ccacttccag tcccttcatc tcttcatttc gaatctcaat
     2101 atccaaaaag aatagctgat gggccccctt ctcatgcact tcagccagca gttggtccgg
     2161 cttgccaaat acctcaaaag agctaggaat gatatgatgt gctttcaaaa gtttctcaat
     2221 cgtcgtttca attctagtct gttgggaaaa atcatcctct aaaacaaata ttctcatctt
     2281 cttactctcc ctctttcaac catttttgac gaatttctct atagcgacgt ctggcatact
     2341 tgacagcgta ttgaccttct tctccgagaa aaaggattct ttcttgaaaa tcaatcgatt
     2401 ttaatttatc aagattaacc aaacaatttc gatgacatct catcaaggtt tccccacatt
     2461 ggttctcaag attacttaaa tttttaagca tattaaaact agcttcttct gtaacaatct
     2521 gtacggtatg ggctttagtt ggatgtgttt ggatatagta gatatcgtcg atgtttactt
     2581 tatagactgt tttctgggtc tgaataatca tatgtttcat tggattctcc tcctaagaaa
     2641 agaataacag aaaaggtcta gcccaagcac acccaatcct aaacagtctt tgaaaactcc
     2701 taaaaggatt attttctaca acatcatgat gagcttacaa ctattttatc ataaaattca
     2761 ctcagcgcaa ttaaagacat ttcaatgaca attaaagatt tgaataaaaa aaaagaatta
     2821 aaagtgataa actaacagta tcaaaacaca gttgccagaa gtaagactgg cacccagatt
     2881 aaaggaggca ttcccatgac agatacagac cccatcaaaa gagctcatac tttgattact
     2941 gacttaaaca aagcctacca agcatgcaaa caggcaagcg ctgacgacct ccgctttcag
     3001 gagcaattaa actctattct tggttttcta gccaaggctg aaacagtgga taatcgattc
     3061 ttgattgaat tggaaaaatt ttaccagact tccagtcttc tcatgggact cagcgctctt
     3121 gatccagatg ctccaactcg cgccgcttgg cgggcctatg accgcttcca ctttgaccaa
     3181 gtcaagacca agttaatact caatgaaaat caaagagcaa actaggaagc tagccgcaag
     3241 ctgtacttga gtacggtaag acgacgctga cgtggtttga agagattttc gaagagtata
     3301 agtctctacg gaccaaccat tatcctgtag taacaaaaaa gaagctgaga cagactgaac
     3361 tcaacttctt ttttagtgtc gtatcagcaa tgttagtcct gcatctgacg ttttacctga
     3421 taaggcaaat acaagacttg taaaaccaaa ccagctccca gtaaggctag aagggctagt
     3481 ttttcttgaa aggtaatcaa cccaacaact aattccacaa gtaaaacaaa actggtcaat
     3541 cctcgaacta cctcctgtaa gcctttttcc ttttgcccct tgtccagcgg aaagagttgg
     3601 gtcaaatact ggtagtcaaa ggcatgatag agggccagca actggaagag caagaggtag
     3661 ttaaagagaa ctaccactgc tgtcgcaatc caagcttgct cgataaaaac ctgcgccagt
     3721 acggaaagta agagaagacg aagactgaga gcaaagaggt cgccatttcg cagataagaa
     3781 cgcagataga gattttgcca aatcttccca ggcaccttct gaacagcctt taagataaag
     3841 tccagatagg cacgacgctt aacgctgttt gaaattccct tgacctgcgt aaagagggca
     3901 aagaaacgaa gcaagacttg cttacgcttg ctttcttgag aaataacata gtcccaatcc
     3961 agtccagttt cagtgaaaaa tttgctggcc ttttgacaaa agtggaaata ttttcctacc
     4021 cccaataaaa gcacatagag cagaaaaact ggcaagccat aacccattgc taaaaataaa
     4081 ggcgcaaata acagcaagaa aagggtctgt acaaagagcc aaaagactag ggaaatgcca
     4141 gtttgacgct tgagatggag cttaatttcc tcttctccaa ctaagagaaa gagcttgtct
     4201 ggagcctcca tataggtggc aattcctccc caaagtaaga gtaaaacaga ggtaattcct
     4261 acaaacaaaa ggataggcca atgattttca ggaaaatctt gcaagagttg actgtactgg
     4321 taggctagaa agcccagcaa gacaagcagg aacaagacaa agtggtcatt gagaacatag
     4381 cgcagataac caagacactc cttacgaaaa gcctgctttc tctttaaaaa caagtctttc
     4441 atagttcctc ctctttggtc agagccaagt aaatatcatt caaactagcc tcaggcatgt
     4501 taaaggcttc gcgcagttgc aggagatttc ccttggcacg cacctctccc ttatgaagaa
     4561 taacaaaggc atcacacatc ttctccgccg aatccagcac gtgggtactc ataagaatgg
     4621 acttgccctt ttgcttttcc acttccaaaa gctgaatcaa gtctgcaatc gccagcggat
     4681 ccagaccaag gaaaggctca tccacgatga aaagactcgg atctaccaca aaagcacaga
     4741 taatcatgac cttctgcttc atcccttttg aaaaatgaac aggaaaccag tctaatttct
     4801 ggtccagacg gaacattttt aacaagggct ctactcgctc aaaagccact ttctgctcaa
     4861 taccataagc catggcaacc gtttcgatat gctctctgag ggtcaattcc tcatacagac
     4921 taggcgtctc aggaatgtag ccaatctgct tgcggtagct agtcgcatct ccttgcatag
     4981 tcaggccatt gatattgatg gagccactat aaggtgccaa cagaccgata atctcattga
     5041 tcgtcgttga tttcccagca ccattgagac caatcaaacc gaccaactgc ccactttcaa
     5101 cagtaaagga cacatctttc aaaacaggaa catgaacata gccacctgtc aggtttttaa
     5161 tttctaacat attttctcca aatctggtat aatgtagcta tattatatca aaattcagta
     5221 cagtagaggt agattttatg tcagattgca ttttttgtaa aatcatcgca ggggaaattc
     5281 ctgcttcaaa agtatatgaa gatgagcagg tccttgcctt tcttgatatc tctcaagtaa
     5341 cactaggaca caccttggtc gtgccaaaag aacactatcg caatcttttg gagatggatg
     5401 ctacgagcgc cagccaactc tttgcccaag taccaaaagt agctcaaaaa gtcatgaaag
     5461 tcactaaggc tgctggtatg aatatcattt ccaactgtga agaagtcgct ggtcaaacag
     5521 tttttcatac tcacgttcac cttgtgcctc gctacagtgc tgacgatgac ctcaagattg
     5581 attttatcgc ccacgaacca gactttgaca aacttgctca agtcgctgaa accatcaaaa
     5641 acgcttaagg agttgctatg aaattatcca acctactgct atttgcagga gctgcagccg
     5701 gaagttatct ggttacaaaa aatcgccaaa ccatcacaga tgaagtcttg aataccactg
     5761 accgcgttca agctatcaaa gacgatgtgg atattatcca aaatagcctg caaatcatcg
     5821 accagcaaaa agaacttatc aaggaatacc aagaagactt gacttacaag tttaaggtct
     5881 tggaaaagga tatccagact agactagcta tgataaaaga aatgcaggga actgaagata
     5941 agtaaaaaga gccccccggc tcttttttaa aacgtaaaca agttatagtg aattgaattt
     6001 agaatagcac atcatagttt ctaaagcatt gttagagatt actttaaaat cctcttatca
     6061 atttgttcat attctatttc aatctactat atatatccca tcaactatgc atcataattt
     6121 aggtaactca tactcaataa aaatcaaaaa gcaaactaga aagctaggca cagactgctc
     6181 aaaacaccgt tttaaggttg tggatagaac tgacgaagtc aactcaaaac actgttttga
     6241 ggttgtggat agaactgacg aagtcagctc aaaacactgt tttgaggttg tggatagaac
     6301 tgacgaagtc agtaaccata cccatgacaa gccgacgctg acgtggtttg aagaaatttt
     6361 tgaagagtat cattcaccat ttcacaacta aatcgaagac tttctgccgt aatgaaaaca
     6421 cctgaaacag cttggtttca gctgtccgga aactttaaga cttaggttca aagtttaggt
     6481 atggaacttc gaaggaggtc gctaccgtcc gtcattactt aggaaagtct taaaaaatct
     6541 ataaagtaaa aagagcccga cagctctttt tgtttatact caatgaaaat caaaaagcaa
     6601 actaggaaac tagccacggg ctgctcaaaa cactgttttg aggttgtaga tacgactgac
     6661 gaagtcagtc acatatatac gacaagccga cgctgacgtg gtttgaagaa atttttgaag
     6721 agtattattc tccatcaaag gcatctttaa tatggtcaaa gaagcctttt ttctttggat
     6781 ttactttcaa gtcaccagca gccgcgaatt ctttcaaggc tactttttgg cggtcgttca
     6841 agcctgtcgg tgttacgaca ttaacagtaa cgtattggtc accaactgca ccgccacgaa
     6901 ggctcggtgc ccccttacca cgtaggcgga atttcttacc agtctgagtt ccctctggaa
     6961 taaccaattc aacatcaccg tgaacagttg gaatatctac tgtatcacca agagccgctt
     7021 ggacaaagtt gaggttgaga ttgtagaaga tagtcgttcc ttcacgttca aacttgtcgc
     7081 tatcttctac agaaactact gcatacaagt ccccataagg tccaccgtta aagcctgctt
     7141 cgccttgacc agcgaggcga atttgttgac ctgtttccac accagcaggg attttcacat
     7201 gtacgctatg agcttgtttc tcatggcctg ttccatgaca ggttgtacat ggatatttga
     7261 tttcttttcc tcgaccgtga cagacatcac aggttacttg gcgacgcatc ataccaagag
     7321 gagtctgcgt atcgacgtta atgacaccag agccatgaca gcgtccacaa gtgactggac
     7381 ttgtccctgg cttagcacca gagccattac atgtacgaca gccagcttca cgatgatact
     7441 taacttcctt ctcagttccg aagatagctt cttcaaaggt caaattgaca cgatactgga
     7501 gatcatctcc ttggcgagga gcgtttggat tgcgcgaaga accgcctccg ccgaagaaac
     7561 ttgagaaaat atcctcaaaa ccaccgaagc cacctgcccc attgaaacca ccgaaaccac
     7621 cagctccacc aaaaccacca ttggcgcctg cagcaccata ctggtcatag gcagcacgtt
     7681 tttggtcgtc actcaaagtc tcataggctt cttgaacttc cttgtacttg tcctcagcac
     7741 caggctcctt gttgatatct gggtgatatt ttttggaaag cttacgataa gcctttttga
     7801 tttcgtctgc cgaagcgttt ttggataccc ccagacgatc ataaaattca gtattgttca
     7861 tacaagatac caagaccgta aaattcgact gaaaaatagg aaatctgacg ctggagagac
     7921 gctcctagac agatttctct tttttccgag aatttaggtc gggttcaatt cctttctttt
     7981 atattgatga gataatactc cagccactcg gccctatccc tttttatcga aaaacagagg
     8041 ctgggttgca acctctgcaa ataagtaaat taactttatt ttgattttaa atatcctata
     8101 agtacagaaa tatttatata tttttgtctc ttcttagtat ttgcctatat aattccaatt
     8161 gatcataaga aatacaacta ctgtccaaat cataccaatt atgaacggta ttacaaattt
     8221 atatgtttgt atttctaatg atatcatagc tgataccatc gccgatacaa atgcagacaa
     8281 caatactgag gtaacaaaat tccttaccgc ttcatcattt ttgacacaac tatcatgctc
     8341 agctaatata atttctctat atatagtcaa acattctact ggaatattat ctataaaacc
     8401 ttttgctgct tcatagttgt cagcttgatt acaaaaatca ttataaactt ttcgtgtatt
     8461 ttttagatac tcatcatcca atacactcat cttatttttc cgtaaactct ccgtctacga
     8521 cgtcatcgcc tgcgtttcct gttgcttgtg cgccttctgc tccttcttga gcttgttgcg
     8581 ctgctgcggc ttgttcgtag agtttcacag caaggccttg agctttttcg ttcaacgctt
     8641 caagtttcgc tttcatgtcg tccaagttgt tgtcttcttg agctttctta aggtcatcaa
     8701 gggcagcttg ggcagcgtca cgttctgcgt cgaagccttt accttcagtt tccttgattg
     8761 tcttttcagt cgcaaagatt gcttggtcta cttcattacg aaggtctact tcttctttac
     8821 gtttcttatc agcttcagcg tttgcttctg catctttcat catgcggtcg atttcttcgt
     8881 cagtcaaacc tgagttcgat tggatgacaa tagtttgttc tttttgagtt ccaaggtctt
     8941 tggccttaac agacacgata ccgttcttgt cgatgtcaaa tgttacttcg atttgaggaa
     9001 ttccacgagg tgcagctggg atatcagtca attggaagcg tccaagagtc ttgttatctg
     9061 ctgccattgg gcgttcacct tgaagaacgt ggatatcaac ggctggttgg ttgtctgctg
     9121 ctgttgagaa gacttgtgat ttagatgttg ggattgtagt gttgcgatcg ataagttttg
     9181 taaatactcc acccattgtt tcgataccaa gtgacaatgg cgttacatca agaaggacaa
     9241 cgtccttgac atcaccagta atcacaccac cttggatagc cgcacccata gcaactactt
     9301 catcagggtt tactgatttg tttggttctt taccagtttc agctttaaca gcttcaacaa
     9361 cggcagggat acgagttgaa ccaccaacaa ggataacttc gtcgatttct gacaagctca
     9421 aacctgcatc tgaaagggct tgacgaactg gaacttttgt acgttcaaca aggtcacgag
     9481 tcaaatcgtc aaatttcgca cgagtcaaag tcatttccaa gtgaagaggt ccagcctcac
     9541 ctgcagtgat aaatggcaag ctgatttgtg ttgaagttac accagaaagg tctttcttcg
     9601 ctttttcagc cgcatctttc aaacgttgca ttgccatctt gtcagtagac aagtcgatac
     9661 cgttttcttt cttgaattct gctaccaagt ggtcaatgat tttttggtca aagtcgtcac
     9721 caccaagttt gttgtcccct gcagttgaca atacgtcgaa gacaccgtca cccaattcaa
     9781 ggatagagac gtcgaatgta ccaccaccaa ggtcaaatac caagattttt tcttctttgt
     9841 cagtcttgtc caaaccataa gcaagagctg ctgcagttgg ttcgttaaca atacgttcta
     9901 cttcaagacc agcaatttta ccagcgtctt ttgttgcttg acgttgagcg tcgttgaagt
     9961 aagccggaac tgtgataaca gctttggtta ccttctcacc aaggtagtct tcagcgtagc
    10021 ctttcaagta ttgaaggatc atagctgaga tttcttgtgg agtgtattct tttccatttg
    10081 cagaaacttt ttcagaagtt cccatcttag atttgataga gataactgta tctgggtttg
    10141 tgactgcttg acgttttgca gcatcaccaa cgatgatttc tccgtttttg aatgagacta
    10201 cagatggagt tgtgcggttt ccttctgggt ttgcgatgat tttgctttca gttccttcaa
    10261 gaactgcaac tgctgagttt gttgtaccta agtcaatacc gataatttta gacatgtgtt
    10321 tttctccttg ttagttaatt ttctattttt ctttttgata ctcttcttaa gtggcgacgc
    10381 cgtcagagct tgacttcgtc aatctctttg actaaacttt gagcctaagg tctcaaaatt
    10441 tgcgcaaata gcgccacggc gaagagtatc gtctttggtt cgcttcgctc actattacaa
    10501 agccaaacat attttatctt tctttatagt ttattgtcgt ttcggacaag ttttctatta
    10561 tgtaaattgc gacacgagga gtcgaaatcg attttatttc gacgacgagt tagtaaggaa
    10621 gctaggcaaa cgccatagcg attgccgttt tctgacgagt tgctttagct accgtcagaa
    10681 ttgccgaatc gaacacgccc taagctctga gtaaaaaaga tagattttct tgtgttcatc
    10741 gaacacttcg tcaatctcct atttttactg cgagcttttt acgggctttg tatcttagtt
    10801 atacaccact accattgctg ggcgtaggat gcggtcatgg agtttgtagc ctttttgaaa
    10861 gacttgagcg atggtatcta ctgggtgttc atcgtctgct gggagagttt ggatggccat
    10921 atggtagtta tggtcaaatt cgccatctac tgcgatttct tcaattcctt cttctttcaa
    10981 agcgtgaatc aagctttctt gcaccatccc caagcccttc ttcacatcat ctgtcaaacc
    11041 ttcaactgca agtgcacgct caaggttgtc aagagatggt aagattgctt ttgccaagtc
    11101 ctggctacga taacgttgca agttttgacg ttcttcattg gcacggcgtt ggatattttg
    11161 catttctgca tgagcgcgaa gatatttgtt ttcgaactca tctgcacgtt catttgccaa
    11221 gtccaactca gacttttcag gagttgtttc ttcagctgtt tccacaactt cctcttcttg
    11281 aacttcttct acttcttcat tttttatatc ttgggccatg ttcgcctcct ttaatgattt
    11341 caatcttaat gtacttcata atgattactg ctgaggtagc ggtaaaaatc tgtcaacttc
    11401 atggtcaaaa cacggttgac cacattgact tgattgatta gctgttggta atccagatta
    11461 actggaccga taatggctag aattccaact ccccgataag gaatgaggaa cttactacta
    11521 atcaccgcta ggtcagctaa acaggactct tgaccgtctg caacacgaac attttgcatc
    11581 tgttcctcac gcaacccctc acgaatctcc aaggccacct tttgcggttg gtcaaagaac
    11641 tgataggctg ctagattggc aaaattcaag agatggacct tgcccgccat cacaatgttt
    11701 tcgttgaaca tttccttaaa gatgtgttca aagagatcga tgacattatc cgttgttgta
    11761 aagtaacgct ggataatctg cggaatctcc gtccgaatct tgtagtgaat atctaaaacg
    11821 gtgtgaccga ggaaacgttc ctgaatgatg ctcttcagtt tcagcaaatc ctcctgcaag
    11881 aagttccttg gaatcagaaa ctgactagta accgttcgcg actcgtctag ggtaaatacc
    11941 gccaaggctg tatgttgccc caaaacaacg atatcaaagg ctgtcaaacg ttgcctgctc
    12001 ggctcaacat ccagtgccac taccgtacag ccactcaggt ctgttagtaa gttagcagcc
    12061 tcttgcagaa tatcctccaa tttgaagaat tcctgatcaa aggctttgac aatctcatat
    12121 acctcatttt cagccagccg gtcaaaatcc agtgagtgtt tcacatagta ctgaaaacca
    12181 gcaacacttg gcatccgacc gcttgaagta tgagccttct caagcaaccc ttgtttttct
    12241 agttccgcca tgtcattacg aatggttgca ctgctagagt taatagactc ttgcaaggct
    12301 tttgatccga caggttcgtg cgttttggta aagatgtcaa taatcagatt taaaatatcc
    12361 tgctgacgct ctgtaaccat ctcatcactc ctctctgtct gatcaattag cactcaatgc
    12421 atccaagtgc taacttatga ttctattata cacccttaga agagaatgtc aagacaaaaa
    12481 ctcaaaaaat tagcactctt ttatcaagag tgctaaaaat caatctgaag acctagaacc
    12541 ttgctattca tctactcggt atttcctttt tagtctgaaa taaccatgaa ttagataaga
    12601 aatcagaaaa gcatataaaa caaaaataag aaagaaagac atagaaagat cctctctaaa
    12661 caaactcgta caaacaacaa gaccacctaa gaaagtagct gtacaggaac gaagtctaga
    12721 tcgcataact tcccatttga gccccttact catatagact tgatcctctg gaagtaaatc
    12781 agaagacgat ttacgaagaa tcgaacaaga acctgctcct atcaattccc aacctctatc
    12841 tctaaaatct tgcagttcat acttatattt tttaagaaat ctagaatcat agatacggta
    12901 gatgacatcg tctggttgac attggtcaaa atagaacaaa ccaaaacgac tagttctata
    12961 cctccaacct ttcaaatgca tctcatgtaa atattcttct tccttgtcca aatcaacaat
    13021 agtgaaaatc cgaaattcta ctctgctatt cattgtctta ccccaaaatt agaaaacatg
    13081 cctggcgtta tttattagat aattctttcc acttttgact caatctccaa aaaatataag
    13141 aaatctgaat cgcaaaaact atcaataaaa cccaatctat tatgaaaatc aaaaacactt
    13201 tccaactgaa agaactacct ccagtgacaa actttgagaa aaacggtagt agagctaaaa
    13261 agagaagtaa aataggaagc atccgcattg ttaaaatccg tttggcataa aaaaatcttt
    13321 atttaaacga aaatattata gcaaaattta cgccagtttt tgaacggctg atgtagatat
    13381 tttatacttt caaaatgttt aaatgtgatt atttattttt gaaaaatagc tcaccagccc
    13441 gactgaaagt gcttatagaa tgataataag tcgcctgccg aaaacagcgg aaaatagcgg
    13501 tgttatgcgg agataatctg acgcgatgcg aaagtatatt gcatacttat tttcaacaat
    13561 ttagcagagt atttttataa gtgtgatata atagaagtat aatttgttct gatagtttat
    13621 tttatggaga agtagatttt tagaatgcgg agggttcaat atggttgagt ttataaagtc
    13681 taagaaagaa atgagtgagg aggatattaa agcaaatttc atcactcctg ctattgtatc
    13741 caaaggatgg aaaaatggtg agcatatcgc ttacgaagaa tacttcactg atggtcgaat
    13801 tgaggttaga ggagataagg ctcgtcgtaa agaaggaaaa aaatcagact attcactgta
    13861 ttaccaattt ggaactcgaa ttgcaattgt tgaggcaaag gataataaac acagcgttcg
    13921 agcaggatta caacaagtta ttgaatatgg agagatttta gatgttccat ttgtttattc
    13981 ttcaaatggt gatggcttta ttgaacacga ccgtatcacg agagaagaac gtgagctgga
    14041 gttagacgaa ttccctactc gtgaagaatt attttctcgt atgacgaagg aaaaaggatt
    14101 gacgtacgaa attacagaag ctatctcaac tccatactat acagacgcct tctcaatgaa
    14161 aacgccacgc tattatcagc aaatagctat caaccgtact attgaaacag ttgccagagg
    14221 acaaaaacga gtaatgtttg tgatggcaac aggaacgggg aaaacgttca tggcttttca
    14281 aattattcat cgccttcgaa aagctggttt ggctaaacga gttttattct tagcagatag
    14341 aaacatctta gtagaccaaa cgatggctga agactttagg ccattcgaaa aggtaatgac
    14401 gaaaattaca ccaaaacttt tgactgctcc tgaaaaatta aattcttttg aaatttatct
    14461 agggctttat cagcaactaa ctggtgaaga tggaactgaa acacattatc aaaaatttga
    14521 caaagacttc tttgatttaa tcgtaattga tgaagcgcac cgtggttcag ctaaggaaga
    14581 cagtaactgg cgtaaggtga ttgattattt cagttctgcg acacagattg ggatgaccgc
    14641 tactcctaaa gaaaccaaga atgcttccaa tacggaatac tttggtgagc caatctatac
    14701 ttatagttta aaacagggaa tcgaggatgg ttttttggct ccatatcgtg ttatgagggt
    14761 taatttagat gtggatgtgg atggttatcg tccagaaact ggaaaagttg atactaacgg
    14821 acaattaata gaagataggt actacggcag gaaagatttt gataaaacca ttgtcattga
    14881 tgatagaacg caaagagttg ccaagtttgt ttctgattat atgaagcaaa gcaatgcacg
    14941 atttgataaa acaattgttt tttgtgttga tattgaccat gccgagcgaa tgcgtgctgc
    15001 acttgtaaaa gagaatctag acttagtcca agaagactat cgttatgtca tgcaagtaac
    15061 tggtgacaac gctgaaggaa aagctcaact ggataacttt atggatgtca attctaattt
    15121 tcccgctatt gtaacaacgt ctaaattatt aacgacagga gttaatgcta aaacatgtcg
    15181 tttgattgtt ttagactcta atatccaatc catgactgaa tttaaacaaa ttattggtcg
    15241 tggcacacgt ctttatcctc aaaaggggaa agaatttttt acgattattg attttcgaaa
    15301 tgttaccaat ttgtttgctg accctgattt tgatggtgag ccagtgaagg tgctggaaac
    15361 aggtgcgaaa acagtcagtg gttctacgcc cggtttcgta gatgaggaag gtgacccagt
    15421 agaaaaatat atcgttacag acaagcaggt taccattctt aattctacta ttcaagtatt
    15481 ggatgaaaac gggaaactga ttaccgaaag cctgaccgac tacactcgga agaatatctt
    15541 aggtagctac gccactttga acgattttat cacagtttgg catacggcgg ataaaaagaa
    15601 gctgatctta gacgaacttt ataaaaaagg agtttatcta gatgctattc gagagtcgga
    15661 gggaatatca gaacaagaaa tcgatgattt tgatttactc ctaaaacttg cctatggtca
    15721 aaaagaatta accaaaacgg aacgtatcaa taaactcaaa caaagcggat atttatataa
    15781 atatagtgag gaagcgcgtg ctgttttgga aattttactg aacaaataca tggataaagg
    15841 tattggagaa ctcgaaagca ttgaaacatt aaaacttcca gaatttcaga tatatggtgg
    15901 aaccttcaaa atcatcaata cttattttgg agataaaaaa cgatatttac aagcaattaa
    15961 agaattggag caagagctat ttacagtagc ttaatgaaag gaaagtatgt caattacatc
    16021 atttgtaaaa agaattcaag atatcactcg aaacgatgct ggtgttaatg gtgatgctca
    16081 acgtattgag caaatgtctt ggttattatt cttaaaaatt tatgatagcc gtgaaatggt
    16141 ttgggaatta gaagaagacg agtatgagtc aattatccca gaggaattaa aatggcgaaa
    16201 ttgggctcat gctcaaaatg gggaacgggt attgacaggc gatgaattac ttgattttgt
    16261 caataacaag ttattcaaag agttgaaaga gcttgaaata acttcaatta tgcctattcg
    16321 aaaaacgatt gttaaatcag cttttgaaga tgcgaacaac tatatgaaaa atggcgtctt
    16381 gttacgccaa gtcatcaatg ttattgatga agttgatttc aatagccctg aagatcgtca
    16441 ttcgtttaat gatatttacg aaaaaattct taaagatatt caaaatgctg ggaactcagg
    16501 agaattttat acgccacgtg cagcgactga ttttattgcc gaagttcttg acccaaaact
    16561 tggagaatca atggcagacc ttgcttgcgg aacaggaggc ttcttgactt cgactctaaa
    16621 ccgtttaagt agtcaacgta aaactagtga agataccaaa aaatataata cagctgtttt
    16681 tggtattgaa aagaaagcat ttcctcatct tttagcagtt acaaatctgt ttcttcacga
    16741 aattgatgac cctaaaattg ttcatggaaa tactttggag aaaaatgttc gtgaatatac
    16801 ggatgatgaa aaatttgaca ttattatgat gaatccacct tttggagggt cagaattaga
    16861 aacaataaaa aataactttc caacagaatt acggagttct gaaacagctg atttatttat
    16921 ggctgtcatt atgtatcgtt tgaaagaaaa tggtcgtgtt ggagttattt tacctgatgg
    16981 ttttctattt ggtgaaggtg taaaaactcg cttgaaacaa aaactggtag atgagttcaa
    17041 cttgcatacg attattaggt tgcctcatag tgtctttgca ccgtatacag gaatccatac
    17101 gaacattctt ttctttgata aaacaaagaa aacagaagaa acttggtttt atcgtttaga
    17161 tatgccagat ggttataaaa atttctcgaa aactaagccg atgaagtcag aacacttcaa
    17221 tcctgttcgt gactggtggg aaaatcgtga agagattctg gaaggtaagt tctacaaatc
    17281 taaatcattt acacctagtg aattggctga gttgaattat aatttagacc agtgtggttt
    17341 tccaaaagag gaagaggaaa tcttaaatcc ctttgagttg attcagaatt atcaagcgga
    17401 aagagcaact ttaaatcata agattgataa tgtattagct gatattttgc agttgttgga
    17461 ggacaaataa tgacaccaga acaacttaaa gcaagtattc tccaaagagc gatggaaggg
    17521 aaattagtgc cgcaaaatcc caatgacgaa cctgcaagtg aattattaaa gagaattaaa
    17581 gctgaaaaag aaaaacttat cagtgaagga aaaatcaaac gagataaaaa ggaaactgag
    17641 atatttcgtg gtgatgatgg gaaacattat gggaagtttg ctgatggaag tactcaagaa
    17701 attgatgttc cttatgatat tcctgatact tgggagtggg tgaggataaa atcaatttat
    17761 tggaattttg ggcaaaataa gccggagaaa tcctttaggt atatagatac gtctagtatt
    17821 gatagaaaaa agaacataat caactacaaa aatctacaat atctttcacc tgaacaagcg
    17881 ccttcccgtg ccagaaaatt agtttcgcag aatagtgtct tattttcaac agttagacca
    17941 tatctaaaaa atattgctgt agttagagaa cttaaagagt atttgatagc tagtacagca
    18001 tttattgttt tggatacttt acttaacgaa acatatttga aatattattt attatcagat
    18061 aattttatca atcgtgttaa taataaatct actggaacaa gttatcctgc aatcaatgat
    18121 tataatttta atctattatt aattgctctc ccccactatc cgaacaacaa cgcata
//
LOCUS       DAVGJL010000026        18069 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.026, whole genome shotgun sequence.
ACCESSION   DAVGJL010000026 DAVGJL010000000
VERSION     DAVGJL010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18069
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.026"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            32..328
                     /locus_tag="VVE42_000783"
     CDS             32..328
                     /locus_tag="VVE42_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEU3073002.1"
                     /translation="MDTPDENGYVADDYRITYLEAHIKAMRDAIYQDGVDLLGYTTWG
                     CIDPVSAGTGEMNKRYGFIYVDRDNVGNGALKRSKKKSFYWYKDVIYSNGASIG"
     gene            449..1393
                     /gene="manA"
                     /locus_tag="VVE42_000784"
     CDS             449..1393
                     /gene="manA"
                     /locus_tag="VVE42_000784"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEU3073003.1"
                     /translation="MSEPLFLQSVMQEKIWGGAKLRDEFGYDIPSEKIGEYWAISAHP
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDDLLTKVPVKAG
                     DFFYVPSGTMHAIGAGILILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTVKADDLRSTLLVSNDFFAVYKWEITGKVDFEKTADYSLLSVLAGQGQL
                     TVDGKNYPIQKGSHFILPSDVEAWTLEGQGLELIVSHP"
     gene            complement(1433..2780)
                     /locus_tag="VVE42_000785"
                     /pseudo
     CDS             complement(1433..2780)
                     /locus_tag="VVE42_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466604.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
     gene            3008..3748
                     /locus_tag="VVE42_000786"
     CDS             3008..3748
                     /locus_tag="VVE42_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU3073004.1"
                     /translation="MYHIKEAAQLSGVSVKTLHHYDKIGLLVPLKSENGYRTYSQEDL
                     ERLQVILYYKYLGFSLEKIAELLKEERTDLLPHLTRQLDYLTRERQHLDTLISTLQKT
                     IQEQKGERKMTIEEKFTGFSYQDNQKYHQEAVEKYGQEVMGQALERQKGHEDEATAAF
                     NQVFQTLAQNLQVGLPATATENQEQAAKLLQAIRTYGFDCSIEVFGHIGKGYVYNPEF
                     KENIDKFGSETAQYTSDAIAVYVQTNAE"
     gene            complement(3777..4232)
                     /locus_tag="VVE42_000787"
     CDS             complement(3777..4232)
                     /locus_tag="VVE42_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEU3073005.1"
                     /translation="MNRREAVEFVNMCMIKNGDKVLVQDRVSPDWSGITFPGGHVERG
                     ESFVDAVIREVKEETGLIISKPQLCGIKNWYDDKDYRYVVLFYKTEHFTGELQSSDEG
                     KVWWEDFENLSHLKLATDDMSDMLRVFLEEDLSEFFYYKNGDDWLYDLK"
     gene            complement(4252..5427)
                     /locus_tag="VVE42_000788"
     CDS             complement(4252..5427)
                     /locus_tag="VVE42_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU3073006.1"
                     /translation="MKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKVDDQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTITDLEAAESPLLTDFPHKALQGISLDDVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSFVKGGEVLSLDVLPQLSEKDLVTFLEENREKS
                     LKNALKTLLPERLAEFFVQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPGLHFAGEVMDINAHTGGFNITSALCTGWVAGSLHYD"
     gene            complement(5486..6331)
                     /locus_tag="VVE42_000789"
     CDS             complement(5486..6331)
                     /locus_tag="VVE42_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU3073007.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAKNIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSVQAAYDE
                     LVKAGYAGGRIVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEEGGLLM
                     GYEID"
     gene            6456..7013
                     /locus_tag="VVE42_000790"
     CDS             6456..7013
                     /locus_tag="VVE42_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU3073008.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFREARKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            7032..7499
                     /locus_tag="VVE42_000791"
     CDS             7032..7499
                     /locus_tag="VVE42_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HEU3073009.1"
                     /translation="MTEKRLAWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLVIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMDDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            7588..8217
                     /gene="upp"
                     /locus_tag="VVE42_000792"
     CDS             7588..8217
                     /gene="upp"
                     /locus_tag="VVE42_000792"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEU3073010.1"
                     /translation="MGKIEVINHPLIQHKLSILRRTDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRDEETLQPVEYLVKLPEDIDQRQIFVVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKALQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            8391..8981
                     /gene="clpP"
                     /locus_tag="VVE42_000793"
     CDS             8391..8981
                     /gene="clpP"
                     /locus_tag="VVE42_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HEU3073011.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN"
     gene            9060..9308
                     /locus_tag="VVE42_000794"
     CDS             9060..9308
                     /locus_tag="VVE42_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEU3073012.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            9410..10570
                     /locus_tag="VVE42_000795"
     CDS             9410..10570
                     /locus_tag="VVE42_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU3073013.1"
                     /translation="MKKKFAPSFVALASVALLAACGEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVVDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QAALTKMKGKDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKNNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP"
     gene            10838..11707
                     /locus_tag="VVE42_000796"
     CDS             10838..11707
                     /locus_tag="VVE42_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU3073014.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLATAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGPISLTNVQLMILAISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLLETFATAFGMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            11711..12667
                     /locus_tag="VVE42_000797"
     CDS             11711..12667
                     /locus_tag="VVE42_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU3073015.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGSKLPTYGAFFGAMLVGALLSG
                     AVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVAS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            12667..13431
                     /locus_tag="VVE42_000798"
     CDS             12667..13431
                     /locus_tag="VVE42_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3073016.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKAKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLEYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            13431..14141
                     /locus_tag="VVE42_000799"
     CDS             13431..14141
                     /locus_tag="VVE42_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3073017.1"
                     /translation="MSMLKVENLSVHYGMIQAVRDVSFEVNEGEVVSLIGANGAGKTT
                     ILRTLSGLVRPSSGKIEFLGQEIQKMPAQKIVASGLSQVPEGRHVFPGLTVMENLEMG
                     AFLKKNREENQANLKKVFSRFPRLEERKNQDAATLSGGEQQMLAMGRALMSTPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAISDRGYVLETGKIVLSG
                     TGKELASSEEVRKAYLGG"
     gene            14449..15105
                     /locus_tag="VVE42_000800"
     CDS             14449..15105
                     /locus_tag="VVE42_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU3073018.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQGLHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKISILPVVDNHQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIVSL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDGSIDLPALKEKFEANGIQVEEIARTSAKVL
                     "
     gene            15213..16308
                     /gene="prfB"
                     /locus_tag="VVE42_000801"
     CDS             join(15213..15284,15286..16308)
                     /gene="prfB"
                     /locus_tag="VVE42_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEU3073019.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNIAAQKTSQELNELKNTYNTFHKMEELQGEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQDKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSFEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            16326..17018
                     /gene="ftsE"
                     /locus_tag="VVE42_000802"
     CDS             16326..17018
                     /gene="ftsE"
                     /locus_tag="VVE42_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEU3073020.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDVPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKRRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            17011..17937
                     /locus_tag="VVE42_000803"
     CDS             17011..17937
                     /locus_tag="VVE42_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU3073021.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYHKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVEANTPNDVKTIAEDAKKIEGV
                     SEVQDGGANTERLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLKI"
BASE COUNT         5544 a         3414 c         3952 g         5159 t
ORIGIN      
        1 tagtattatc gtagaaaatg gactcggtgc tatggataca ccggatgaga atggttatgt
       61 agcagatgac tatcggatta cttacttaga ggcccacatc aaggccatgc gagatgccat
      121 ttaccaagac ggggttgact tgcttggtta tacgacttgg ggctgtatcg atccggtttc
      181 agctggcaca ggggaaatga ataagcgcta tggctttatc tatgtagatc gagataatgt
      241 aggaaacgga gctctcaaac gtagcaagaa gaagtccttc tattggtaca aggatgtcat
      301 ttatagcaat ggtgcaagca tcgggtagag cacatttgtt tactattttt ataaaatctt
      361 ctccctgctt tataagatgt aaaaaagagt tcaattcgaa ctcttttttt gctataatga
      421 ggggagaaaa atcagacagg agatcgacat gtcagaacca ttatttttac aatcagttat
      481 gcaagaaaaa atctggggtg gagccaagct acgtgatgag tttggctacg acatcccaag
      541 tgaaaaaatc ggagaatatt gggccatctc agcccatcca aatggagtct ctaaagttgc
      601 caatggtcgt tacgagggaa cagatcttgc tactttgtat gcggaacacc gtgaattatt
      661 tggcaatcgt ccagaacctg tatttccact tttgaccaag atcctcgatg ccaacgactg
      721 gctcagtgtc caagttcacc cagacgatgc ttatggactc gagcatgaag gcgaactcgg
      781 aaaaacagaa tgctggtaca ttatcgcagc ggatgaaggt tcagagatta tctacggtca
      841 caatgccaag tcaaaagaag aactccgcca gcaaatcgag gacaagaact gggatgactt
      901 gttgaccaaa gtccctgtta aggctggaga tttcttctat gtaccaagcg ggactatgca
      961 cgctatcggg gcgggtatct taatccttga aacccagcag tctagcgata ccacctatcg
     1021 cgtctatgac tttgaccgca aggatgacaa aggcaacttg cgtgaacttc atcttgaaaa
     1081 atccatcgat gtcttgaaca ttggtgagcc agcaaatagc cgtcctgtaa ctgttaaagc
     1141 agatgatttg cgttccactc tccttgtatc taatgatttc ttcgcagttt acaagtggga
     1201 aattactgga aaagttgact ttgaaaagac agctgactac agcttattga gtgtcttggc
     1261 tggtcaaggt cagctaactg ttgacgggaa aaattatcca attcaaaaag gcagccactt
     1321 tatcctacca agtgatgttg aagcttggac tctggaaggg caaggtttgg aattgattgt
     1381 tagccatcca taaaaagaaa gggcctgagt tcactactca agtccttttt gattacataa
     1441 attgggcaat gaagaccaca atgatgatga ttggaatgac gaaacgaaga aacgaagaag
     1501 gaagagccag acttggaaca gtccttgttt ccatgctctt tcatcgagat ggagttcctc
     1561 cattgcaaga gcctttttaa agatatagcc tgtaaaaagt gaaaggtaga gagctccaaa
     1621 tggcatgagg agattggaaa ccaagaagtc catagcgtca aagaaggtct taccaaaaat
     1681 gtgaacatcc gccatgacac cgtaagatag ggctgaagga atgccaaaga caaaggtcaa
     1741 aattcctaaa ataacactcc atttggcacg tttgctgtta tcctggttgg tgatattgtc
     1801 tacattgatt tccagcatca cgacagaaaa agtgactgtc gcaaaaagga agagcaagag
     1861 gaagaggacg tagaaaatgg ttccaaaagg catcttgtca aagagttgag gcaagacgat
     1921 aaagagcagg ctgggtcccc cttcagactg gatattgaag ggggatcgag cttggaaaat
     1981 ggctagacct gccatgatgg atatcgagat attcatggct acgatggaga ttcctgactg
     2041 gactagattg gttttcttgt ctaagtaaga agcataggtc aacatgactg taacccctag
     2101 tgagagggca aagaaagatt gtcccagagc atagaggaga ccagtgctag tcagttttga
     2161 aaagtctggt ttgaggaagt aaagaacccc ttccatggca tttggcaaac tgagagagcg
     2221 accgatgata aaaacaaaga cgataaagag caggggcatc atgactttcg aagctctttc
     2281 aatccctttt tgaaccccac gtgatacaat gaagatattc aataggataa aggccgcttg
     2341 agctcctagg gcaatggctg gatttgaaat gattgaagta aataactgag cataatcacc
     2401 cgttccacca agttggaaca atttcccaaa ctcaatacct agatagacta gaatccatcc
     2461 tccgataaca ctgtaaaaag ataagaggat aaagagggca aaggcgccaa tccaaccgat
     2521 aaagttgtac ttgttattct tgcccagttt tccaaaggtt ttgatagcgg aaacgccagc
     2581 actacggcca agggcaaact cagccagcag gagagggaaa ccgattaaaa tagtggaaat
     2641 gagaaagatt agtaaaaagc ctccaccgcc attagcagca gtcatgtagg gaaacttcca
     2701 aacggaacca agcccgatgg ccagccagca gatgctagaa taaaaccaag tttcgacccc
     2761 cattgtgatt tttctgacat agttaaactc tgatctcctt tttcaaaata agaaaaagct
     2821 acttgagttg tcaaatagcc ttctgtttga ttgatagact tgactatcct atcatgtttt
     2881 ctaaggtctg tcaagaaaat cattttcaag ttttcacacc tttctcaaaa aagttaaaaa
     2941 attttctcaa aaacgcttga ctctgaccta aggggagggg ttatactatc attgtaagga
     3001 ggaaatcatg taccatataa aagaagctgc gcagctttca ggtgtctctg tcaagaccct
     3061 gcatcactat gacaagatag gactcttggt ccccttaaag tcggaaaacg gctatcgaac
     3121 ctacagtcaa gaggatttgg aacgccttca ggtcattctt tactacaaat atctaggctt
     3181 ttctttagag aaaatagcag agctgttaaa ggaagaaagg acagatttat tgccccattt
     3241 gactaggcag ttggactatc taactcgcga aaggcaacat ctggatacct tgatttccac
     3301 cttgcaaaaa actattcaag aacaaaaagg agaaagaaaa atgaccattg aggaaaaatt
     3361 cacgggattt agctatcaag acaatcaaaa ataccaccaa gaagcggtag agaaatatgg
     3421 tcaagaagtc atgggacaag cgctcgaacg ccaaaaaggt cacgaagacg aggctacggc
     3481 cgcctttaac caagtctttc aaactttggc acaaaatctt caagttggtt tacctgcaac
     3541 agcaaccgaa aaccaggagc aagcagccaa gctcttgcaa gccattcgca cttatggatt
     3601 tgactgctct attgaggtat tcggtcatat cggtaaaggt tacgtctaca acccagagtt
     3661 taaggaaaac attgacaagt ttggttctga aacagcccag tacacgtcag atgccattgc
     3721 ggtttacgtt cagacaaatg cagaataaat aggctaggaa tttcctagcc tattttttac
     3781 ttcaaatcat aaagccagtc gtcaccgttt ttgtagtaaa agaattcact gagatcttct
     3841 tctagaaaca cacgaagcat atcagacata tcatcggttg caagttttag atgagaaaga
     3901 ttttcaaagt cctcccacca aactttccct tcgtctgaag actggagttc accagtaaag
     3961 tgttctgtct tgtaaaaaag gacgacataa cgataatcct tgtcgtcata ccagtttttg
     4021 ataccacaga gttggggttt ggaaatgatc agaccagttt cttctttcac ttcacgaatg
     4081 acagcatcga caaaggattc gccacgttca acatgaccac caggaaaagt aatgccagac
     4141 cagtcgggac taactcggtc ttggaccagg accttatctc cgtttttaat catacacatg
     4201 ttaacaaatt cgactgcctc tcttctgttc attcttcaca acctttaatc tttaatcata
     4261 atgcagactt cccgccaccc agccggtaca gagggcagaa gtgatgttaa agccacccgt
     4321 gtgggcattg atatccataa cttcgcctgc aaagtggagg ccaggtacca gcttactttc
     4381 aagggtttta ggattgattt ccttgagact gactccaccc ttggtaacaa aggactttgc
     4441 aagggacatt tttccagtta caggaatttt aagttcttta atggactgga caagttgttc
     4501 tcgttccttt tcagtcagtt gtttgacttt ttcaggatat ccttgtacaa aaaattcggc
     4561 caagcgttct ggtaacaagg tttttaaagc gtttttcaag gatttttccc gattttcttc
     4621 tagaaatgta accaagtcct tctcagaaag ttgaggcaaa acatcgagtg agagaacctc
     4681 cccacctttg acaaagctag acatgcgtag ggcagcagga cctgacaaac caaagtgggt
     4741 aaagagtaaa tcatgagtga tgacatgctt accataactt agggtcacat cgtccagaga
     4801 aataccttgt aaggctttat gtggaaaatc tgttaataaa ggactttcag cagcctcaag
     4861 atcggtgatg gtatgcttaa aatggcgagc aatctcgtga ccaaaaccag tcgaaccagt
     4921 cgaaggataa gacttaccac ctgttgtgac aatgagtttc tcacaagtga aggtttgatc
     4981 cgctgactta aggacaaact ggtcatctac ttttttaaca gaaacgattt ctatttgagt
     5041 agcaacttga ccacctagtt cggtgatttt cttttccaaa gcttcgataa tagtccgaga
     5101 cttgtcactg gctggaaaga cgcgtccgtg gtcttcgacc ttaagtttaa caccattttc
     5161 tgtaaaaaag ttgatgatgt catgattatc gaactgggag aaaacactgt aaagaaagcg
     5221 tccgtttcca ggaattccag ctagcaggtt gtctaagcta ccattgttgg tcacattgca
     5281 acgtccccca ccagtcccag ctaatttttt tccaagtttc cgattttttt cgatgaggag
     5341 ggttttctgt ccataaaagc tactggaaat cgtagccatc ataccagcag gtcccccacc
     5401 gatgacaata gtatcaaaat gtttcatagc tctattgtac cacaaaaaaa caagagatga
     5461 tggtcacctc ttgtcaagaa tgcaattaat caatttcata gcccatcagc aaaccgccct
     5521 cttctgcata gaaactgcag agaccagagg ttggtagaat tttaatatcc gcttgtggga
     5581 aggtttcacg gattcgctct gagagctgtt gacaacattt ttcgttattg cgttgggcca
     5641 tgacaatacg gccaccagca tatccagctt ttactaactc atcataggca gcttgaactg
     5701 atttctttga tccccttgct ttttgtagca attcgagagt cccagtttca ctagcttttc
     5761 cgaccatacg aatgttgaga aggccaacga ccgtaccgat aagcttgctc aaacggccgt
     5821 tcttcaccaa gttatcgact ttggctagga caaagagcaa cttagttttt tcttgatagg
     5881 cggtgatagc ttcaaccact tcttcaaaag acaagccctg gtcaatcaag tcattcaatt
     5941 tttctacgag taggtcaact tcaccaccag cagataaact atcaatcaca tgaatcttag
     6001 tgtcaggatg gtcttccaga taaatattct ttgctagttg agcactattg tgactgccag
     6061 aaagggtacc tgtgatggtt actaggaaaa tgtttttggc accttcaaat gctcgcaaat
     6121 agtcatctgg gcttggacaa gctgattttg aagcttctgc agttgcatac atggtttcca
     6181 tcatttggtc aatatcgaga ctggcgtcat caacaaagac ctgatcagct acttgaatgg
     6241 ttaaggggac gcttacaaag gttgtgttaa tagctggtgt tggcagttga cgataatcac
     6301 aaccagagtc agcaataatt ttccaagtca tagaaattct ccatctttgt cagttatata
     6361 ttgacaaagg ttctgtcttt ttttacaatt atatcatgaa aacccttgaa acaaaagcat
     6421 catccgtctg ttgtgacaag tagaaagaaa atgttatgtc tgaacgtaga atctctgaaa
     6481 agtctcttga aaatctcaga aaatcaaacc aagaatccaa tttattaacc agagaagcca
     6541 ttgaaacagc cctcttgcaa ctcttggaaa aaaaggagct gaccaagatt agtatttctg
     6601 aattggtcaa acgtgcgggt gtttcgcgtg cggcctttta tcgcaattat gactccaaag
     6661 aggaaatttt agagagcgtc tttaaacgaa ctgtccacaa tattatggaa cagctgcatc
     6721 attacgattt aaagacagac ctttatttgg tttgggttca ccttttccgg gaggccagaa
     6781 aggaagctag agtgattcaa ttggccttgg attaccatct ggaaaaaatc tttgtccaag
     6841 ccatgcagga atttctagaa aaataccatg ggaaatcaaa aggtgtcagc tcttatcttc
     6901 attccttctg gagctcagcc atcgtctctg tccttctaaa atggatcaag gatggcatga
     6961 aggtaccagc tgaaaagatt gcagatttag gtttaccatt ttttaaaaaa tagagaaaaa
     7021 ggagaaaaga gatgactgaa aaaagactgg cctgggatga gtattttgca gcccaagccc
     7081 tactaattgc gaatcgttcc acttgtaaac gtgctaaagt gggcgcgatt ctggtaaaag
     7141 ataataaggt tatttccact ggttacaatg gttcggtgtc agggactgag cattgtattg
     7201 atcacgaatg tttggtcatt gaaggccact gtgttcgcac ccttcacgct gaggtcaatg
     7261 ctatccttca aggtgctgag cgtggtgttc ccaaaggctt tacagcctat gtaacccatt
     7321 ttccttgtct caactgtaca aaacaattgc ttcaggtcgg ctgcaagcgt gtggtttata
     7381 tcaaccagta ccgaatggac gactacgccc aataccttta tcaagaaaaa gggacagaat
     7441 tgacccattt accacttgag acagtacaga cagctcttaa agaggctgat ctaatgtaaa
     7501 aattatcaaa aataaatggt ttagaaagat ttttaaaccg ttttttggta taataagaag
     7561 aataaattga aagaaggaat tccaaaaatg ggaaaaattg aagttattaa tcacccactg
     7621 attcaacaca aattgtcaat cttgcgtcgt acagatactt ctacaaaagc ttttcgtgag
     7681 ctagtagatg agattgcaat gttgatgggg tatgaagtac ttcgtgatct tccactagaa
     7741 gatgtggaaa tcgaaacacc aattacaaaa acagttcaaa aacaattggc aggtaagaaa
     7801 ttggccatcg tcccaatctt gcgtgcaggt atcgggatgg ttgatggtct cttgagcttg
     7861 gttccagctg ctaaagttgg ccacatcggt atgtaccgtg atgaagaaac acttcaacca
     7921 gttgaatact tggtgaaatt gcctgaggac attgaccaac gtcaaatttt tgtagtagac
     7981 ccaatgttgg caacaggtgg ctcagcaatc ttggctgttg attctcttaa aaaacgtggc
     8041 gcatcaaata tcaaatttgt ctgccttgta tccgctccag agggtgtaaa agcccttcaa
     8101 gaagctcatc cagatgtaga aatctttaca gcagccttgg atgaacgttt gaacgaacac
     8161 ggttatatcg ttccaggtct tggagatgct ggagaccgct tgttcggtac aaaataagat
     8221 cgaaaagaag aatgacttgg aaaagtattt ccagtcacga aaggaggttg ggtttttgtt
     8281 tctgtctaat gaaagcagag caaaaatttg accttttttg accaagatat tataatagtc
     8341 ttatcttcag tcatttgacc aacaaaatta aaactcaaaa ggagaaatga atgattcctg
     8401 tagttattga acaaacaagc cgtggagaac gttcttacga tatttactca cgtcttctca
     8461 aagaccgcat cattatgctg acaggtccgg ttgaagacaa tatggctaac tctgttattg
     8521 cccaactgct tttcttggat gcccaagata gtacaaaaga tatttacctt tatgtcaata
     8581 caccaggtgg ttctgtttca gctggtttgg caatcgtaga taccatgaac tttatcaagg
     8641 cagatgtcca aaccattgtt atgggaatgg ctgcatctat gggaacagtc atcgcatcaa
     8701 gtggagcaaa aggcaaacgt ttcatgcttc caaatgctga atacatgatt caccaaccaa
     8761 tgggcggtac aggtggtggt acccaacaaa ctgatatggc tatcgctgca gaacacttgc
     8821 tcaaaactcg taataccttg gaaaaaatct tggctgaaaa ttcaggtcag tcaatggaaa
     8881 aagtccatgc agatgcagaa cgtgataact ggatgagcgc ccaggaaaca cttgaatatg
     8941 gctttattga tgaaattatg gccaacaatt cattgaacta atgatgatag aaggcaaact
     9001 cgactgggtt tgcttttttt ggtataatag ggagagattt cttagaaaga ggatttatca
     9061 tgtttgaaaa agtcaatcgc tctggcttga ttatctatct ttactataat cgtgatgcca
     9121 aaaaactgca ggattatgga gatattacct atcattccaa gaaacatcgt tacttacaac
     9181 tctatgttcc aactcaagaa gtggagcaat tggtcggacg cttgagcaag gaaaaattta
     9241 ttaaaaaagt tagggtttgt catatccaag agttggaaac accctttgtg ggcaatcttt
     9301 atcgatagga aaacgctatc atcgaaaaag ttcaaggaaa acattgacaa ttttctgata
     9361 attcggtata ttcttaacag actatttaag aaataaggag acaaaaaaga tgaagaaaaa
     9421 atttgcccca tcgtttgtgg cgcttgcaag tgtagcactt cttgcagcct gtggagaagt
     9481 gaagtctgga gcagtcaaca ctgctggtaa ctcagtagag gaaaagacaa ttaaaatcgg
     9541 gtttaacttt gaagaatcag gttctttagc tgcatacgga acagctgaac aaaaaggtgc
     9601 ccaattggct gttgatgaaa tcaatgccgc aggtggtatc gatggaaaac aaatcgaagt
     9661 agtcgataaa gataataagt ctgaaacagc tgaggctgct tcagttacaa ctaaccttgt
     9721 aacccaatct aaagtatcag cagtcgtagg acctgcgaca tctggtgcga ctgcagctgc
     9781 ggtagcgaac gctacaaaag caggtgttcc attgatctca ccaagtgcga ctcaagatgg
     9841 attgactaaa ggtcaagatt acctctttat tggaactttc caagatagct tccaaggaaa
     9901 aattatctca aactatgttt ctgaaaaatt aaatgctaag aaagttgttc tttacactga
     9961 caatgccagt gactatgcta aagggattgc caaatctttc cgcgagtcat acaagggtga
    10021 aatcgttgca gatgaaactt tcgtagcagg tgacacagac ttccaagcag cccttacaaa
    10081 aatgaaaggg aaagactttg atgctatcgt tgttcctggt tactataatg aggctggtaa
    10141 gattgtaaac caagcgcgtg gtatgggaat tgacaaacca atcgttggtg gtgatggatt
    10201 caacggtgag gagtttgtac aacaagcaac tgctgaaaaa gcatcaaaca tctactttat
    10261 ctcaggcttc tcaactactg tagaagtttc agctaaagct aaagccttcc ttgacgctta
    10321 ccgtgctaag tacaatgaag agccttcaac atttgcagcc ttggcttatg attcagttca
    10381 ccttgtagca aacgcagcaa aaggtgctaa aaattcaggt gaaatcaaga ataaccttgc
    10441 taaaacaaaa gattttgaag gtgtaactgg tcaaacaagc ttcgatgcag accacaacac
    10501 agtcaaaact gcttacatga tgaccatgaa caatggtaaa gttgaagcag cagaagttgt
    10561 aaaaccataa tagaaaaatg ttgaaatagg gaatgagcct ttgactcact ccctgtttcg
    10621 atatttaata ctcttcgaaa atctcttcaa actgcgtcaa cgtcgccttg gattatatat
    10681 gtgactgact tcgtcagtct tatctacaac ctcaaagcag tgctttgagc aacctgcggc
    10741 tagtttccta gtttgctctt tgattttcat tgagtataag aacctatcaa aaagtgaggg
    10801 aaacccctcg gaattataaa tagaaagagt gaatcttatg ctccaacaac tcgtaaatgg
    10861 tttgattcta ggtagtgttt acgcgctgtt agccctagga tataccatgg tttacggaat
    10921 tatcaagctc atcaacttcg cccatggtga tatttatatg atgggagcct ttatcggtta
    10981 tttcttgatc aattctttcc aaatgaattt ctttgtagcg cttattgtag ctatgctagc
    11041 gacagctatt cttggtgtcg tgattgagtt tcttgcttac cgacctttgc gccactctac
    11101 tcgtattgct gttttgatta cggctattgg ggtttctttc ctattggagt atggaatggt
    11161 ctatctggtt ggtgccaata cccgtgcctt ccctcaagcg attcaaacag ttcgatatga
    11221 tttgggacca attagcttaa caaatgtgca gttaatgatt ttggctattt ccttgatttt
    11281 gatgattttg ttacaagtca ttgtccaaaa gactaagatg gggaaagcca tgcgtgcagt
    11341 atcagtagat agcgacgcgg cgcaattgat ggggatcaat gtaaaccgta cgattagctt
    11401 taccttcgct ttgggttctg ctcttgcggg tgcggctggt gttctgattg ctctttatta
    11461 taactctctt gagcctttga tgggggttac tccaggtctt aaatctttcg ttgccgcagt
    11521 acttggtggt atcggaatta ttcctggtgc ggctcttggt ggctttgtga ttggtctatt
    11581 ggaaaccttt gcgactgcct ttgggatgtc agatttccgt gatgccattg tttatggaat
    11641 cttgttgttg atcttgattg tccgcccagc tggtatcctt ggtaagaatg tgaaagagaa
    11701 ggtgtaaacg atgaaggaaa atttaaaagt taatattcta tggttactcc ttttgttagc
    11761 tggctatagc ttgattagtg tactggtttc agtcggagta cttaatctat tctatgtaca
    11821 gattttacag caaattggaa ttaatattat tttggctgtt ggtctcaact taatcgttgg
    11881 tttttcagga caattttcac ttggtcatgc tggtttcatg gcgattggtg cctatgcagc
    11941 agctattatt ggttctaaat taccaaccta cggtgccttc tttggagcta tgcttgtagg
    12001 ggctttgctt tcaggagcag ttgccttact tgtcggcatt ccaaccttgc gcttgaaggg
    12061 ggactatctt gcggtagcaa ctctgggtgt ttctgaaatt atccgtatct ttatcatcaa
    12121 tggtggaagc cttacaaatg gtgcggcagg tatcttaggg attcctaact ttacaacttg
    12181 gcaaatggtt tacttctttg tcgtgattac aaccattgca accttgaact tcttgcgtag
    12241 cccaattggt cgttcaaccc tctctgttcg tgaagatgaa atcgctgctg agtcagttgg
    12301 ggttaatacg actaaaatta aaatcatcgc ttttgtcttt ggtgccatta ctgcaagtat
    12361 tgctgggtca cttcaggcag gatttatcgg gtctgttgta ccgaaagatt acaccttcat
    12421 caactcaatc aacgttttga ttattgttgt atttggtgga ctcggttcca ttacaggtgc
    12481 gattgtttcg gctattgttc tgggaatttt gaatatgctt ctccaagatg ttgctagtgt
    12541 gcgtatgatt atttacgctt tggccttggt attggtaatg attttcagac caggtggact
    12601 ccttggaaca tgggaattga gcctatcacg tttctttaaa aaatctaaga aggaggaaca
    12661 aaactaatgg cattacttga agtaaaacag ttaaccaaac attttggtgg tctaacagct
    12721 gttggagatg tgactcttga attgaacgaa ggggaactgg ttggattaat cggtccaaac
    12781 ggagctggga aaaccaccct tttcaacctt ttgaccggtg tttatgaacc aagtgaggga
    12841 acagtaaccc tagatggtca ccttttgaat gggaaatcac cttataagat tgcctctttg
    12901 ggacttggac gtactttcca aaatatccgt ctttttaaag atttaacagt tttagacaat
    12961 gttttgattg cttttggaaa ccatcacaaa cagcatgttt ttactagttt cttacgctta
    13021 ccagcttttt acaagagtga aaaagaatta aaggctaaag ctttggaatt gttgaaaatc
    13081 tttgatttag atggtgatgc agagactctt gctaaaaatc tttcctacgg acaacaacgt
    13141 cgtttggaaa ttgttcgtgc ccttgctacg gaacctaaaa ttctcttctt agatgaacca
    13201 gcagcaggta tgaacccaca ggaaacagcc gaattgactg agttaattcg tcgtatcaaa
    13261 gatgagttta agattacaat catgttgatt gaacacgata tgaatctggt catggaagta
    13321 acagaacgta tctacgtact tgaatatggc cgtttaatcg ctcaaggaac tccagacgaa
    13381 attaagacca ataaacgcgt tatcgaagct tatctaggag gtgaagccta atgtctatgt
    13441 taaaagttga aaatctttct gtgcattacg gtatgatcca agcagttcgt gatgtaagct
    13501 ttgaagttaa tgaaggagaa gttgtttccc ttatcggtgc caacggtgca ggtaagacaa
    13561 ctattcttcg caccttgtca ggtttggttc gaccaagttc aggaaagatt gaatttttag
    13621 gtcaagaaat ccaaaaaatg ccagctcaga aaatcgtggc aagtggtctt tcacaagttc
    13681 cagaaggacg ccacgtcttt cctggcttga ctgttatgga aaatcttgaa atgggagctt
    13741 tcttaaagaa aaatcgtgaa gaaaatcaag ctaacttgaa gaaggttttc tcacgctttc
    13801 ctcgtcttga agaacggaag aaccaagatg cagccactct ttcagggggg gaacaacaaa
    13861 tgcttgccat gggacgcgcc ctcatgtcaa caccaaaact tcttctttta gatgaaccat
    13921 caatgggact tgccccaatc tttatccaag aaatttttga tatcattcaa gatattcaga
    13981 agcaaggaac aacggtcctc ttgattgaac aaaatgccaa taaagcactt gcaatctctg
    14041 accgaggata tgtactggaa acagggaaaa tcgtcctatc aggaacagga aaagaactcg
    14101 cttcatcaga agaagtcaga aaagcatatc taggtggcta aaacaatcca gtggattgtt
    14161 ttagtcggca gatggagatt acgaagtaat catcaatata gtccggggga cctttttagt
    14221 cggtagattg agattgcaaa caaatctgca tctacattga aagcttaatt tctaataatt
    14281 gaaaaaatcg aatgaaaaat ttcttacctt cattcacaga gctcgatttc agagctcttt
    14341 ttgctagctt attcatactt ttctgaattt cgaaaaagaa atgtaagcgt ttgatagatt
    14401 tacaaaaaga ttgtataata gggataagaa tagaaaagga gaagtctcat ggcagttaaa
    14461 gattttatga cccgcaaggt agtttatatt agtccagata caacagtatc tcatgcagca
    14521 gatttgatga gagagcaagg tttgcaccgt ctgcctgtta tcgaaaatga tcaattagtt
    14581 ggtttggtga ctgagggaac cattgcacaa gcaagtccat ctaaagcaac aagtctttct
    14641 atctatgaga tgaattatct tctgaataag acaaaagtaa aagatgtcat gattcgcgat
    14701 gttgtcactg tctcaggcta tgctagtcta gaagatgcaa cttatctgat gttgaaaaat
    14761 aagattagta ttctccctgt cgtagataac catcaagtat acggagttat tactgaccgt
    14821 gacgttttcc aagcctttct tgaaattgca ggttatggcg aagaagggat tcgtgtacgc
    14881 tttgttacag aagatgaagt tggtgttctt ggaaaaattg tttctttgat tgtagaagaa
    14941 aatttgaata tctcccatac agtcaatatt ccgcgtaagg atggtaaggt gattatcgaa
    15001 gtgcaaatcg atggatcaat tgatttacca gccttgaaag aaaaatttga agcaaatggt
    15061 attcaagtgg aagaaatcgc tcgcacttca gcaaaagtct tgtaagaagg gaagcccaaa
    15121 ggcttctttt ttcatgaaaa ggggattaga gaaaaagatg gaaagaaatg ataaaatatg
    15181 ctataatgaa ataatgtaaa aaaggagtat ttatggacat ttcagtaatt cgtcagaaaa
    15241 ttgacgcaaa tcgtgaaaaa ttagcttctt tcagggggtc tctttgacct cgaagggcta
    15301 gaggaagaga ttgccatctt ggaaaacaag atgacagaac ctgatttttg gaacgataat
    15361 attgcggccc aaaaaacgtc gcaagaattg aatgaattaa aaaacactta caataccttc
    15421 cataagatgg aagagttgca gggtgaagtc gaaattttat tggatttttt ggctgaagac
    15481 gagtcagtgc atgatgaact ggtagcgcag ttagccgaac ttgataagat aatgaccagc
    15541 tacgagatga ctctactctt gtcagaacct tatgaccaca acaatgccat cttggaaatc
    15601 catccaggtt ctggtggtac tgaggcgcag gactggggtg atatgttgct tcgtatgtat
    15661 actcgttatg gtaatgctaa aggctttaaa gtggaagtgt tggattacca agcaggtgat
    15721 gaggctggta ttaagtcggt aactttatca tttgaagggc ctaatgccta tggtctcctc
    15781 aagtcagaaa tgggtgttca ccgcttagtg cgaatctcac catttgactc tgccaaacgt
    15841 cgccatacct ctttcacatc tgtagaagtg atgccagaat tggatgatac tattgaagtg
    15901 gaaatccgtg aagatgatat caagatggat accttccgtt caggtggtgc cggtggacaa
    15961 aacgtcaata aggtttcaac aggtgtacgt ttaacccaca ttccaactgg aattgttgtc
    16021 caatcaacag tggatcgtac ccagtatgga aatagagatc gtgccatgaa gatgttgcag
    16081 gctaagctct atcaaatgga gcaagataag aaggctgcgg aggtagattc tctcaaaggt
    16141 gagaaaaagg agatcacttg gggaagccaa atccgttctt atgtcttcac gccttatact
    16201 atggtaaaag atcaccgaac tagctttgag gttgctcagg tagataaggt tatggatggg
    16261 gacctagatg gttttatcga tgcttatctc aagtggcgaa ttagctaaga tagaaaggaa
    16321 ctcacatgtc aattattgaa atgagagatg tcgttaaaaa atacgacaac ggaacgactg
    16381 ctctacgcgg tgtttcggtt agcgttcaac cgggggaatt tgcttacatc gtaggacctt
    16441 caggagcagg gaagtcaact tttattcgtt ctctgtatcg tgaagtaaaa atcgataaag
    16501 gaagcctatc agttgctggt tttaatctgg ttaagatcaa aaagaaagat gtcccgcttc
    16561 tacgtcgtag tgttggggtt gtcttccagg attataaatt gttaccaaag aaaactgtct
    16621 atgaaaatat tgcttacgct atggaagtaa tcggggaaaa tcgccgtaat atcaaaagac
    16681 gagtgatgga agttttggac ttggttggat tgaagcataa ggttcgttct ttcccaaatg
    16741 aactctcagg tggggagcaa cagcggattg cgattgcgcg tgcaattgta aataatccca
    16801 aagtattgat agctgatgag ccaacaggaa atctggatcc ggataattca tgggaaatta
    16861 tgaatctctt ggaacggatt aacctacaag gaacaactat tttgatggcg actcataata
    16921 gccagattgt aaataccttg cgccaccgtg tcattgccat tgaaaatggc cgtgtcgttc
    16981 gtgacgaatc aaaaggagag tatggatacg atgattagta gattttttcg ccatttattt
    17041 gaagccttaa aaagtttgaa acgaaatggt tggatgacag tagctgctgt cagttcagtc
    17101 atgattactt tgaccttggt ggcaatattt gcatctgtta ttttcaatac agcgaaacta
    17161 gctacagata ttgaaaataa tgtccgtgta gtagtttata tccgaaagga tgtggaagat
    17221 aatagtcaga caattgaaaa agaaggtcaa actgttacaa ataatgacta ccacaaggta
    17281 tatgattctt tgaagaacat gtctacggtt aaaagtgtta ccttttcaag taaagaagaa
    17341 caatatgaaa aattaaccga gataatggga gataactgga aaatctttga aggagatgcc
    17401 aatcctctct atgatgccta tattgtagag gcaaacactc caaatgatgt aaaaactata
    17461 gccgaagatg ctaaaaaaat tgaaggtgtc tctgaggttc aagatggcgg tgccaataca
    17521 gaaagactct tcaagttagc ttcatttatc cgtgtttggg gactagggat tgctgctttg
    17581 ttaattttta tcgcagtttt cttgatttca aataccattc gtattaccat tatttcccgc
    17641 agtcgcgaaa ttcaaatcat gcgcttggtc ggagctaaaa acagttatat ccgtggaccg
    17701 ttcttgttag aaggagcctt tatcggttta ttgggagcta tcgcaccatc tgttttggtc
    17761 tttattgttt atcaaattgt ttaccaatct gtcaacaaat cgttggtagg gcaaaatcta
    17821 tccatgatta gtccagattt atttagtccg ttgatgattg ccctactatt tgtgattggg
    17881 gttttcattg gttcattggg atcaggaata tccatgcgcc gattcttgaa gatttaggta
    17941 aaatagctgc ttttatgagg agattgtaaa atctcctttt ttgctacaag agtttttgaa
    18001 aagagatgcg tagaagaaaa gagcttccaa agaagtcccc cagagaagac ttcttgatgg
    18061 ctaatttga
//
LOCUS       DAVGJL010000027        17713 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.027, whole genome shotgun sequence.
ACCESSION   DAVGJL010000027 DAVGJL010000000
VERSION     DAVGJL010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17713
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.027"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(<1..1138)
                     /locus_tag="VVE42_000804"
     CDS             complement(<1..1138)
                     /locus_tag="VVE42_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEU3073022.1"
                     /translation="MKITEYKKKDGSIVYRSSIYLGTDTVTGKKVKTTISGRTKRELK
                     AKALQAQIDFEKDGSTVYKAVEIKTYAELVENWLETYCHTVKKSTLMGTKFKIDKYLL
                     PAFGNYRLDKLTPPIIQKQVNQWAKDYNQLGKGFQEYPLLHSLNKRILKYAVSLQAIP
                     FNPARDVIVPRRKEKEGQKLKYLDDDNLKKFLTYLEQLPNTYKNFYDTVLYKTLLATG
                     LRIRECLALKWSDIDLKNGTLDVNKTLNIIKEITGPKTKSSIRVIDLDNKTVLMLRLY
                     KARQSQIGKEMGLTYEKVFSNSFDKHIDARMLSFRLVKHLERAGCPRFTFHAFRHTHA
                     SILLNAGLPYKEIQTRLGHAKLSMTMDIYSHLSKDNQKNATSFYE"
     gene            complement(1304..2155)
                     /gene="dinD"
                     /locus_tag="VVE42_000805"
     CDS             complement(1304..2155)
                     /gene="dinD"
                     /locus_tag="VVE42_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HEU3073023.1"
                     /translation="MELQQYDELTFENIKHIDENGVEFWYARELQTILEYMEWRNFNQ
                     VIDKAKIACENSGKRVVANFVDVNKTVQLNFGTREIADIKLSRYACYLIVQNGDPRKE
                     VIALGQSYFAIKTRQQELHDDFNQLTEDQKRIAIRDEIKHHNKSLSESAGNAGVKNFG
                     RFHNSGYQGLYGGLNMQDIHNLKELNEGEHILDFMGSAELAANLFRATQTDEVLRRRN
                     IKGEELANNTHFNVGRTIRNTMKELGTTMPENLPTPRESIQELKNKQKEFEKQADNNQ
                     LSLFDDM"
     gene            2418..3182
                     /locus_tag="VVE42_000806"
     CDS             2418..3182
                     /locus_tag="VVE42_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073024.1"
                     /translation="MNKIKITLIFTSLAWTFVNLYQALQSPTVINLTSFVGIVPIIAA
                     LYSEIDWLYIHWNKFRAYIFLKTVSFTPKSSKFLKETITLNQLEKQIRSVLKENDYSI
                     NEATFSKTHEDIYIDIESTRGIRNKLSISLHPESNGQRLIFKADYQLAYRDVKKQWDS
                     FLSLRDNLFSIYSREESSKERFDVTIKTDKHRKYNPFYRMTVRHLGKKEVKKFDLQFK
                     DNDLSVKTTLNKIYGTSNNRQDIEKLINEYIPLSRL"
     gene            complement(3177..3872)
                     /locus_tag="VVE42_000807"
     CDS             complement(3177..3872)
                     /locus_tag="VVE42_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073025.1"
                     /translation="MGATSISVRQVYEFGIPNGTTDIKKANVTIGSITTTDQTLTIEG
                     KVYTKFKGSYTKTIDDEILVYNSDADEYQQYDNYQKAFEFDIFYSQTDQLLFLTTTTP
                     TAKKFLKELEKIDTLNINYTSLKFDLMAISNMMPQTKGVSFNSTDAGVSSKSFSGDEV
                     DVNDEAIEALANDEATKIIGTLDILGKSRTIMLTQSGTILSFTSLTDISEQKEYPMLE
                     FSIATLMKIGVLS"
     gene            complement(4001..4624)
                     /locus_tag="VVE42_000808"
     CDS             complement(4001..4624)
                     /locus_tag="VVE42_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU3073026.1"
                     /translation="MNRLKELRQKNELSQKEIAEILGFSLRSFQRMENGESQIKPEKA
                     QQLADFFGVSVGYLLGYIDDSEIYDDEVVIEPEEGIVLAYSNKRSNKKLQEKMFKDFI
                     TFFRDNTIFVSDEEILSLYSMVQAANLNNSTPRGTHFNNLLFSNTDEAKQIIKSYSFV
                     FGNEFARNDLDEQIHGYISEETRTKEKTEKLLKVLQSAYGERDYLDY"
     gene            4843..5043
                     /locus_tag="VVE42_000809"
     CDS             4843..5043
                     /locus_tag="VVE42_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEU3073027.1"
                     /translation="MSKLRGYRVMLGLTQQQMADKLKISLQSYNNKELGKTPFNDKER
                     LAIKSMVAEIKSDITIDELFYS"
     gene            5059..5829
                     /locus_tag="VVE42_000810"
     CDS             5059..5829
                     /locus_tag="VVE42_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073028.1"
                     /translation="MKRNYTRVIDEMRTTHGLNLVAIGQRIGIDPRTVGKWAQGKHKP
                     NKESRKRLNKLYREVKQDMTTQQKGLCDFFGNFYPKGEQHNKKEVLEVVTTQRFGSKY
                     FDVYGDIQNPLFVAVEVAEMIEIQNTTDLLKRVDDDEKLTYVISRAGQKREMNMLTEF
                     GVYEVLSQSRKPLAKEFKKVVKHILKEIRLNGYYMAGELVEEPQTTIKAPDTLAEAER
                     YYIDTLAKAIAEAQNMDDKSRLTSKLTRFMQEVEPQWN"
     gene            5820..6450
                     /locus_tag="VVE42_000811"
                     /pseudo
     CDS             5820..6450
                     /locus_tag="VVE42_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424668.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
     gene            6720..6887
                     /locus_tag="VVE42_000812"
     CDS             6720..6887
                     /locus_tag="VVE42_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEU3073029.1"
                     /translation="MTDKKKMGRPTTDPKNLKMTIRFNDEQSRKIENYASQNNLTKSE
                     VIRKAVEQLPE"
     gene            7043..7471
                     /locus_tag="VVE42_000813"
     CDS             7043..7471
                     /locus_tag="VVE42_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073030.1"
                     /translation="MRKKHQVVKFKDIAEKLPELEGKRLEEITKVLGYRNLESCRTNL
                     CKLKQNNRLDFTLEKGVYSKFALLDGTVKEELEDKELSERGRYLKSVDRYKAMLNAFS
                     IAFDSTVKAETRQKAEHDGLKTLDRIPDKHYALLYDMMEG"
     gene            7475..7621
                     /locus_tag="VVE42_000814"
     CDS             7475..7621
                     /locus_tag="VVE42_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073031.1"
                     /translation="MEQYTKRASALIITFNRGVISQDEFIEEFTKLREKVVKAVSEAK
                     NDKL"
     gene            7608..7757
                     /locus_tag="VVE42_000815"
     CDS             7608..7757
                     /locus_tag="VVE42_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073032.1"
                     /translation="MTNYDRAKRFYSRATYVLSDFLHGRIDEQEFRKQWQRLADKAVN
                     ETKKN"
     gene            8151..8651
                     /locus_tag="VVE42_000816"
     CDS             8151..8651
                     /locus_tag="VVE42_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073033.1"
                     /translation="MIADQLKAISTNLEEIQEILVGDGTALAKREISRVLNDLDDVYN
                     ELTSTEYREQQETLKEQTERMKQRVVAEVIGGLEEREESYYRNYWGDTKFIKLLSNFD
                     GFLFLNTYLAEITKENTYPMEQSQLLNYVWELIAVDIAKKKRGKKNVLDSWTDSSLYT
                     RGMMID"
     gene            8663..8803
                     /locus_tag="VVE42_000817"
     CDS             8663..8803
                     /locus_tag="VVE42_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073034.1"
                     /translation="MTLDLGKMTQAEFDEVMADIKARNPNLLQFITDFLDGKVTPGEV
                     DD"
     gene            8865..9080
                     /locus_tag="VVE42_000818"
     CDS             8865..9080
                     /locus_tag="VVE42_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073035.1"
                     /translation="MNELDISNTQAAVLIVILIGLLLYLNHRDRKKSAQIERENQQTI
                     ETPSEDLNPEYGRYIQLAGVNVWGGME"
     gene            9077..9373
                     /locus_tag="VVE42_000819"
     CDS             9077..9373
                     /locus_tag="VVE42_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001106820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073036.1"
                     /translation="MNYIVKIYLDSEKIPDEPYFTKEIFLATCMGNATKLAVTMGLLT
                     KQALDLETKTAILKALDTIIETQKAILQGVASGQITFVNKSKGEEELDEPIRER"
     gene            9351..9692
                     /locus_tag="VVE42_000820"
     CDS             9351..9692
                     /locus_tag="VVE42_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEU3073037.1"
                     /translation="MSLSENDKRVLRLIKVGAENSITGSEISLTTKLTERTVRDIIKR
                     LVVKHNIPIVGVRCGVFSGYFIPANKGELLDGAKAFYNQVQEESKRLAVLMNSDLESY
                     KETLKEVGGYV"
     gene            9685..9960
                     /locus_tag="VVE42_000821"
     CDS             9685..9960
                     /locus_tag="VVE42_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073038.1"
                     /translation="MFSLSRESEHDLTHGILEVVERYLEAREKVPPRLTKLINRVELK
                     EELKISDNTLNKWESQGLRRYQPPDDGARIIFYLVDDIRRFMGVENG"
     gene            10101..10961
                     /locus_tag="VVE42_000822"
     CDS             10101..10961
                     /locus_tag="VVE42_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase alpha helix C-terminal domain-containing
                     protein"
                     /protein_id="HEU3073039.1"
                     /translation="MTIYEARGFQSNLVYPFDKIEPFQYIERFKPLVVPESADTEEYK
                     RTQAPYCLSGKVMPEKNGSYKRNNSSLIYRDLIFLDYDDIQGTTEDFIEAVSSALFGY
                     SYILYPTIKHSIEKPRFRLVVKSNNVMNEATYKQVVKEIADKIGLPFDMASLTWSQLQ
                     GLPVTTGDPATYQKIVEHGLDYPVPKVEPRAKQGTTERYKPRVSGQRSMTMRIIDTLF
                     NGFGDEGGRNVALTRFVGLLFNKWVDCDLETAYELTKIANSVTVEPLPIEELDRSFSS
                     IARAEYRKRG"
     gene            11005..12495
                     /locus_tag="VVE42_000823"
                     /pseudo
     CDS             11005..12495
                     /locus_tag="VVE42_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809634.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
     gene            12815..13012
                     /locus_tag="VVE42_000824"
     CDS             12815..13012
                     /locus_tag="VVE42_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073040.1"
                     /translation="MAMNICQRVIRLEETVERISEALDGFIENANHNAIVSNKNIERI
                     QESFEIVFKELDELKAVPDKH"
     gene            13105..13629
                     /locus_tag="VVE42_000825"
     CDS             13105..13629
                     /locus_tag="VVE42_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073041.1"
                     /translation="MTLKTFSDKAKTFNFTYEFKDQDTAQVAGSALMGYMIGTYEVPS
                     ISITYKNKGTLVAEYVEDKKLNYIFKRICDSFKDYYKQPVNDEAFEERYKRERVLQLK
                     ESEDFESLLNKVTDYELELLEYAERLLSDKPILMDSMTAFGTLEMLGNESINLFQKLD
                     VEGEYKGLADYSGQ"
     gene            13699..14202
                     /locus_tag="VVE42_000826"
     CDS             13699..14202
                     /locus_tag="VVE42_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073042.1"
                     /translation="MKRLAIETITKPMKLRGISKGIAELDGQGLEIDLDNLEIDFGGE
                     SFDLARIPGTKGGYRYFFLCPDCGRRCRLLYKRYLYFSCGTCLDIHKSTLNRSKTDCQ
                     YYWELALKEARKVEPGWSPRRGGYMFDGFPERPKYMKRDRYHKHYKKFLKYIEKGDRF
                     WLNGLRL"
     gene            14784..15194
                     /locus_tag="VVE42_000827"
     CDS             14784..15194
                     /locus_tag="VVE42_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEU3073043.1"
                     /translation="MTPEQVKETLEGVKWINKEIEGLYLELATLESGIIKKQELSNTR
                     VQTSRVNTAENNLISVLKLKEDTLQRIERLTEERMEISRLIDKLANPLERSVLRLFYL
                     NVLEAWQVAEEIDRSKTTVYLIRQKAIEHLANVG"
     gene            15350..15742
                     /locus_tag="VVE42_000828"
     CDS             15350..15742
                     /locus_tag="VVE42_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEU3073044.1"
                     /translation="MELDKFKTMMNVRERMTYFLRFQRMAGSENQVTIDEEAWKLVLP
                     YRWDLSGEHEKAIREGLEIFAQDINSIENKRARKYFIIHYCYMRKKTMSECVEMAATS
                     STSYHRYKQIAVLNFARIHQNGELEVYK"
     gene            15796..15954
                     /locus_tag="VVE42_000829"
     CDS             15796..15954
                     /locus_tag="VVE42_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073045.1"
                     /translation="MIEDFLIDLENIQLEDMEIDFNMDIDIDLNIDFEIDVDLDDLDL
                     TLLQELEA"
     gene            complement(16383..16577)
                     /locus_tag="VVE42_000830"
     CDS             complement(16383..16577)
                     /locus_tag="VVE42_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEU3073046.1"
                     /translation="MYQVGNFVEMKKSHACTIKSTGKKANRWEITRVGADIKIKCSNC
                     EHVVMMGRYDFERKMNKIID"
     gene            complement(16642..>17713)
                     /gene="dnaN"
                     /locus_tag="VVE42_000831"
     CDS             complement(16642..>17713)
                     /gene="dnaN"
                     /locus_tag="VVE42_000831"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEU3073047.1"
                     /translation="SSKNAIPILSTVKIDVTNEGITLIGSNGQISIENFISQKNEDAG
                     LLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQNQIVLTSGKSEITLKGKDSEQYP
                     RIQEISASTPLILETKLLKKIINETAFAASTQESRPILTGVHFVLSQYKELKTVATDS
                     HRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDDIETVEIFFANNQILFRSENISF
                     YTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSMERARLLSSATQNGTVKLEIKDGV
                     VSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYLIDSLKALNSEKVTISFISAVRP
                     FTLVPADTDEDFMQLITPVRTN"
BASE COUNT         5976 a         2999 c         3705 g         5033 t
ORIGIN      
        1 tttcataaaa tgaagtagca tttttctgat tgtctttgga taggtggcta taaatatcca
       61 tggtcataga aagttttgca tgaccgagcc gtgtttgtat ctctttgtaa ggtagtccag
      121 cattgagcaa gatactagca tgagtgtggc ggaaggcgtg gaacgtaaaa cgagggcatc
      181 cagcacgttc caaatgtttt actaacctga aactaagcat tctagcgtct atatgcttat
      241 caaagctatt agagaatact ttctcatagg tcaaccccat ttctttccct atttgggatt
      301 gtcttgcttt atagagccgt agcatgagca ccgtcttatt atccaagtca attaccctaa
      361 tacttgattt agtcttaggg ccagtgattt ctttgataat atttaacgtt ttatttacgt
      421 ctagtgtgcc gttttttagg tcaatatcag accatttgag ggctaaacac tcacgaatac
      481 gcaaaccagt cgctaaaagc gtcttatata gcaccgtatc gtagaaattt ttgtaagtgt
      541 ttggcagctg ctccaggtaa gtcaagaatt tttttaagtt atcatcatcc agatatttca
      601 gtttttgccc ttctttttcc ttacgacgtg gcacgataac atcacgggca gggttaaagg
      661 ggatagcttg caaagatacg gcatatttaa gtatgcgttt gtttagagag tgaaggagcg
      721 gatattcttg gaatcctttg cctagttgat tatagtcttt tgcccattga ttgacttgct
      781 tttgaatgat tgggggcgtc agtttatcca gcctataatt tccgaaagct ggaagtagat
      841 atttatctat tttgaatttg gtacccatta aggtcgattt tttaaccgta tggcagtacg
      901 tttctagcca attctccacc agttcggcgt aagtctttat ctcaactgct ttatatactg
      961 tagacccgtc tttttcaaag tcgatttgag cctgtagcgc tttggctttg agttctctct
     1021 tggttcgccc tgatatagtt gtcttgactt tcttacctgt tacggtatcc gtacccagat
     1081 agatactaga gcggtagact attgacccgt ctttcttttt gtattctgtt attttcatga
     1141 tgataaacct ttctaaaata catcagcagg caagccatta tcaaaaaggt tttatatcat
     1201 gctagggctt cacagtttgc cctgtattcg attttagata gtcagacggt aaataatacc
     1261 agagttagaa acaaggcgga tatggggtta atatggccca ctattacata tcatcaaata
     1321 atgaaagctg gttattatcg gcttgctttt caaattcttt ctgtttattt ttcaactctt
     1381 gtatactttc gcgaggagta gggagattct ccggcattgt tgttccgagt tctttcatgg
     1441 tatttctaat ggttcgccct acgttaaaat gtgtattatt cgctaattcc tcacctttta
     1501 tattcctccg gcgtagaact tcatcggtct gagtagcacg gaacaagtta gcggccaatt
     1561 cagcacttcc cataaagtct aaaatatgtt cgccttcatt tagttctttg aggttatgga
     1621 tatcctgcat attcaaccca ccatataagc cctgatatcc tgagttgtga aatcggccaa
     1681 agtttttaac accggcattg ccagcagatt cagataatga tttattgtga tgttttattt
     1741 catcacggat agcaatgcgt ttctgatctt cagtaagttg attaaaatca tcgtgtagtt
     1801 cttgctgacg ggttttaata gcaaaatatg attgccctag tgcaatgacc tcttttctag
     1861 ggtcgccatt ttggacaatc aaatagcagg catagcgaga gagtttaata tctgcaattt
     1921 ctcgcgttcc aaaattaagt tgtacagttt tgttgacgtc aacaaaattg gcaaccacgc
     1981 gctttccgga gttttcacag gctatttttg ctttgtctat aacttggtta aagttacgcc
     2041 actccatata ttctagaatg gtttgtagtt ctctggcgta ccaaaattct actccatttt
     2101 cgtctatgtg tttgatattt tcaaaagtta attcatcgta ctgttgaagt tccataataa
     2161 ggtatccttt ctaaaatatt gtaagtggtt gacatttgtt gacatcaaca aaattagtag
     2221 atatggggct tatatgggct tgaagctcga acgaagcccg agaatccacg cgcttcagga
     2281 ttgttgaacc ttgtggcgcg tggtatggtg taagtcgcat aatcggcctt tttggccatc
     2341 ctaaaaaaaa atattaggga tgaggttcca ggggttttta tgttacaata ctatcaaaga
     2401 ctatttggag cgataatatg aataaaataa aaattactct tatatttact tctttggcat
     2461 ggacatttgt gaatttatat caagctttac aatcacctac tgtgataaat ctgactagtt
     2521 tcgtcggaat tgttcccatt attgctgctt tgtattccga aattgattgg ctttatattc
     2581 attggaacaa atttagagcc tatatatttt taaaaacagt ttcttttaca ccaaaaagtt
     2641 ctaagttttt aaaagaaact attacactta accaattaga aaagcaaatc cgatccgtac
     2701 ttaaggaaaa tgactattcc ataaatgaag ctactttcag caaaactcat gaagatatct
     2761 atatagatat agaatcaaca agaggtataa gaaataagtt gagtattagt ttacatccag
     2821 agagcaatgg gcagagactg atattcaagg ctgactatca attagcgtat agagatgtaa
     2881 aaaaacagtg ggactctttt ttgtcactaa gagataatct gttttctatt tattctaggg
     2941 aagaaagtag caaagaacgg tttgatgtaa ccattaagac tgataaacac aggaaatata
     3001 atccatttta cagaatgact gtacgacatc ttggaaaaaa agaagtaaag aaatttgatt
     3061 tgcaatttaa ggacaatgat ctatcggtca aaactaccct aaataagatt tatggaacat
     3121 ctaataatcg tcaagacata gaaaaattaa ttaatgaata tataccattg tcacggttat
     3181 gatagcactc caattttcat tagtgttgcg atagaaaatt ctagcatcgg atattctttt
     3241 tgttcggaga tatctgtcaa agatgtgaaa gagagaatag taccagactg agtcagcatt
     3301 atagttcttg atttacccaa tatatccaat gtacctatta tctttgttgc ttcatcattt
     3361 gccaaagctt caattgcttc atcgtttaca tcaacctcgt ctcctgaaaa tgacttactt
     3421 gagacaccgg catcagtact attaaaacta actccttttg tttgtggcat catgttggaa
     3481 attgccatta aatcaaattt taacgatgta taattaatat ttagtgtatc aattttttcc
     3541 aattctttta aaaatttttt cgctgtagga gttgttgtag tcaaaaataa taattgatct
     3601 gtttgcgaat aaaaaatatc gaattcaaag gctttttggt aattgtcgta ttgttgatat
     3661 tcgtcagcat cagagttata taccaagatt tcatcgtcga ttgtctttgt ataacttcct
     3721 ttaaatttag tgtatacttt tccttctatg gtcaatgtct ggtcagttgt tgtaattgag
     3781 ccaatggtaa cattggcttt tttgatgtct gtagtaccgt tcggtattcc aaattcataa
     3841 acttgtctaa cagaaataga agtagctccc ataatatttt ctcctttttt aatttactaa
     3901 tgttaaatat tcttctttgc ccctcacact caaagatggc gatggcgaat gtggaggtta
     3961 attctctcta tatatactca cgacttcgcc gatagtaaat ttaatagtcc aggtagtcgc
     4021 gttctccata tgctgattgc aaaactttta agagtttttc ggttttttct tttgttctgg
     4081 tttcttcaga gatatacccg tgaatttgct catctaaatc atttctagca aactcgttac
     4141 cgaaaacaaa tgagtaagat ttgattattt gtttggcttc gtcggtatta gaaaaaagta
     4201 aattattaaa atgtgtacct cttggtgtgg aattattaag attggcagcc tgcaccatag
     4261 agtacagaga taaaatttct tcatcactga caaagatagt gttatcacgg aaaaatgtaa
     4321 tgaagtcttt aaacatcttt tcctggagct ttttgttaga acgtttatta gaatatgcta
     4381 aaacaattcc ttcttcaggt tctattacaa cttcatcgtc atatatttca gaatcatcaa
     4441 tataccccaa cagatatcca acgcttaccc cgaagaagtc agctagttgc tgggcttttt
     4501 ctggtttgat ttggctttct ccgttttcca ttcgttggaa tgaccttaaa gaaaatccta
     4561 aaatttcagc tatttctttt tgagatagct cgtttttttg tcttaattct ttcaatctat
     4621 tcatgcttta tcacaccttt cacagatgat tataacccta tgtttaaaaa aagtcaaaaa
     4681 atgacgtaaa aaaatcaaaa aaactattga caacgtctaa aactgacgtt ataataaaat
     4741 tcaagcttag gagacgtcat aaaacgacgc ctagcgaccc ctccaacctt tcacactttc
     4801 aatctattca tggaggggga ttttttagaa agggggaaag ctatgagtaa actacgaggc
     4861 tatcgggtta tgttaggact aactcagcaa caaatggcgg acaagctaaa aatttctttg
     4921 cagtcgtaca acaacaaaga attaggtaaa acgccattca atgataaaga acgtctagcg
     4981 attaagtcaa tggttgcaga aatcaaatca gatataacca tagatgaact attttatagc
     5041 tagaaaggca ggcaagcaat gaagaggaac tatacaaggg tcatcgatga aatgcgaacc
     5101 actcacgggc tgaacttggt agctattggc caacgaatag gcatagatcc ccgaacagtt
     5161 ggcaagtggg cacagggcaa acataagccc aacaaggaaa gcagaaaaag actaaacaag
     5221 ttatacagag aggtgaagca agacatgaca acacaacaaa aaggtttatg tgattttttt
     5281 gggaacttct atccaaaagg agaacaacac aacaaaaagg aagttttgga agttgtaaca
     5341 actcaacgat tcggatctaa atattttgat gtttatggag acattcaaaa tccgttattt
     5401 gtagcggtag aggttgctga gatgattgaa atccaaaaca ccacagattt actaaaacgg
     5461 gtagatgatg atgaaaagct gacctatgta atatctaggg caggtcaaaa aagagaaatg
     5521 aatatgctga cagagttcgg agtctatgaa gttttatcgc agtcacgcaa accactagca
     5581 aaagagttta aaaaagtggt caagcacatt ctgaaagaaa tccgactaaa tggctactac
     5641 atggctgggg aactggtgga agaaccacag accactataa aagcccctga cacgttggca
     5701 gaggcagagc ggtattatat cgatacacta gccaaagcca ttgcagaggc tcagaatatg
     5761 gacgacaaga gccgtctgac aagcaagtta acccgattca tgcaggaggt agaaccacaa
     5821 tggaactagt ctacatggac ggaaagaaag agccgtatac tacgagtgag atcatcgctg
     5881 aatgtgctgg agtcactcat cacacaatac aagaactttt aaggaagcat aaagctgatt
     5941 ttgaaagcta cggaattatc gcatttgaaa tgcgtaaatt agacggccga gggagaccaa
     6001 tgaaaatata tcgcctaaat gaacaacaag cgaccttgct gatcacttat ttaaagaata
     6061 ctgaaacctg tacggcggtt caaaatgaac ctagtcaaag cattctttga aatgcgtgag
     6121 gagctttcta agttccgtat gcagagggcg ctagaaaagc caaaaagaaa aaccttgcat
     6181 gacagtatag agacatggga ggcaaaaccc aagcacgcgc atagcaccat gaacaacctg
     6241 ctactaaaag cagtaaccga caggaacgct aagcagttaa gggaagaacg tggaggctat
     6301 aatggcatcg atagcttgac cagtgacgag ctggagcaat atcaagcctt tgaagacatg
     6361 gtaatagcca tgattggctt gaatatgagt tatcaggaaa tcaaggctat ggcattcaga
     6421 aataaaaaaa cacgccaaag aagcgcgtga ttgcaacaaa aaaggcttag caataaccac
     6481 accgcaaagc ctttcacact aagactaaaa cgaatttaac aaaaagcagg caagctatta
     6541 ttaaagaggt tttagtaaga tttataccta gattatagca taatacagcg gtttccgcta
     6601 gtacattgaa aataaaataa ggtacgctgg gaagatttta aatttttttc ggtttagggg
     6661 ttgacatttg actgacataa gttatataat acttttgtca gacgaaagga gggaacctga
     6721 tgactgacaa gaaaaaaatg ggaagaccga caactgaccc taaaaactta aaaatgacaa
     6781 taaggtttaa tgatgagcaa agcagaaaaa tagaaaatta tgctagtcag aataatctta
     6841 ctaagtctga agttattaga aaggcagttg agcaacttcc agaataaaaa agtccgccta
     6901 gattgtgtta agtttggcga cctaacaacc tagacggaga gcccccacga atgaggtacg
     6961 taaatatctt atcatgcgta ctcttatttg tcaaacatag aaatagagtg cgctttttgc
     7021 gtacccaaaa atgaggtaaa caatgagaaa gaaacatcaa gtagtaaaat ttaaagatat
     7081 agctgaaaaa ttgccagaat tagagggtaa gcgtctagaa gaaattacta aagtattagg
     7141 ttaccgtaac ctagagagtt gcagaaccaa cctctgcaag ctgaaacaaa acaatcgcct
     7201 agattttaca ctagaaaaag gagtttatag caagttcgca ctcttggacg gtacggtaaa
     7261 agaggaattg gaagataaag aattgtcaga acgtgggcgc tatttgaaga gcgtagaccg
     7321 atacaaggca atgctgaatg ctttttctat cgcctttgat agtacggtca aggcggaaac
     7381 cagacaaaaa gcagaacacg acggcttgaa aaccttggac agaatcccag ataaacatta
     7441 cgccctactt tatgacatga tggaggggta acagatggaa caatacacaa aaagagcaag
     7501 cgccttgatt ataactttta atcgtggggt tatatctcaa gatgagttta ttgaagaatt
     7561 tacaaaacta agagaaaagg tagtaaaagc agtaagtgag gcaaagaatg acaaattatg
     7621 atagagccaa gcgattttac agccgtgcaa cttacgtcct gagcgacttt ctgcatgggc
     7681 ggatagatga acaagaattt agaaaacaat ggcaacggct agcagataaa gccgtaaacg
     7741 aaacgaagaa aaactaagaa aaccgaagag caggcaagca attagaaaag gttttgaaaa
     7801 gcatagagcg ccaactcttt aaactggtac tttctcacgc tttcaatttg gcgagtctga
     7861 gcgtgaggct agtggcaaga aaaactgtat aagaaacaac tcaaaaagcc ccacgctcag
     7921 aagtttggcg accgagagcg tgaggcatgt gacaggaaaa aagcattaag aagccatcag
     7981 ggcaattaca caacatagaa acagaggtac aagtatggaa gaaacaacat acgatattat
     8041 cgctaaaagc ttggatagaa ttagtatgga attacaccaa gctgacgaaa ataacgattt
     8101 tctagaatat gggttttatc aggacaatta aaaggccaac ggaggaaaat atgatagcag
     8161 atcaattaaa agctatttca acaaatttag aagaaataca agaaattctt gtaggagatg
     8221 gaactgcatt agctaaaaga gaaatcagca gggtattgaa cgacttggac gatgtttata
     8281 atgaactaac tagcacagaa taccgtgaac aacaggaaac acttaaggaa cagacggaaa
     8341 ggatgaaaca gagagtagtt gctgaagtaa taggaggact agaagaacga gaagagtctt
     8401 attacagaaa ttattggggt gatactaagt ttataaagct actttccaac tttgatggat
     8461 ttcttttctt aaacacttac ctagctgaaa taactaaaga aaatacctac ccaatggaac
     8521 aaagtcaatt attgaattat gtatgggaac tcatagcggt tgatatcgca aagaaaaaac
     8581 gaggaaaaaa gaacgttttg gattcatgga cagattcatc cttatatact cgtggaatga
     8641 tgattgatta ggaggcaatg cagtgacact agacctaggc aaaatgacac aagcagaatt
     8701 tgatgaagta atggctgaca tcaaggcaag aaatccaaac ctattacagt tcatcactga
     8761 ttttttagat ggaaaggtaa cacccggaga ggtggacgac tagttgaata tggagcgagc
     8821 tgaacaagtg gaatatatta agaattatca agcgagggca tgacatgaat gagctagata
     8881 taagtaacac acaggctgct gtcttaatcg tgattttgat tggcttactg ctttatttaa
     8941 accaccgaga ccgcaaaaaa agcgcccaaa ttgagcgaga aaaccaacag acgatagaaa
     9001 cacctagcga ggatttaaac cctgaatatg ggcgatatat ccagcttgca ggcgtcaacg
     9061 tatggggagg gatggaatga attacatagt gaagatatac cttgatagtg aaaaaatacc
     9121 agatgaacct tattttacta aagagatttt tctagctacg tgtatgggca atgcaacaaa
     9181 gttagctgta actatgggct tgttgacaaa acaagcgttg gatttagaaa ctaagacagc
     9241 tatcttgaaa gcccttgata caatcataga gactcaaaaa gctattttgc aaggggtagc
     9301 gagcggacaa ataacctttg taaataaaag taaaggagag gaggaactgg atgagcctat
     9361 cagagaacga taaacgagta ttaagattaa tcaaggtagg ggctgagaac tccataacag
     9421 ggtcagaaat tagcctgaca accaagctaa cagaaagaac agtacgtgac attatcaagc
     9481 gtctagtagt taagcacaac atccctattg taggggttag atgtggcgtt tttagtgggt
     9541 actttatccc agcgaataaa ggcgagttac tagatggcgc taaggctttt tacaaccaag
     9601 tacaggagga gagcaagcgc ctagcggtgt tgatgaatag cgacctagaa agctacaaag
     9661 aaaccttgaa ggaggtgggc ggatatgttt agcttaagcc gagaaagtga gcatgatttg
     9721 acgcatggca ttctggaggt agtggaaaga tacctggaag cgcgtgagaa agtaccgcca
     9781 agattgacca agttaataaa tagagttgaa ctcaaagaag agttaaaaat tagtgacaac
     9841 acgctgaata agtgggaaag tcagggttta agacgctatc aaccgccgga cgatggtgct
     9901 agaataatat tttatttagt ggatgacatt cgtaggttta tgggggtgga aaatgggtag
     9961 atataacaca cacccagcgc aaggcggagg tcattatcat gacattaaat tgtataagcg
    10021 ccgtggcaga tctttggaac agttccaaga acaaaagcta ctgaagaagt ttaaaaaaaa
    10081 acgcaaaaag gggcgatagt atgacaattt atgaggctag aggtttccag agtaatcttg
    10141 tctatccatt tgataagatt gagccgttcc agtacattga acgctttaag cctttggtag
    10201 tccctgaaag tgctgatacg gaggaataca aaagaaccca ggcgccatat tgtttaagtg
    10261 gtaaggttat gccggagaaa aacgggagtt acaaacgaaa caactccagt ctaatctatc
    10321 gggatttgat tttccttgat tatgacgata tacaggggac gactgaggac ttcatagaag
    10381 ccgtttctag cgctttgttt ggctactctt atatcttata cccgactatc aaacatagca
    10441 tagaaaagcc ccgttttcgc cttgtggtga aatctaacaa cgtgatgaac gaggcaacct
    10501 ataagcaggt agtaaaagag attgctgaca agattgggct accctttgac atggccagtt
    10561 taacctggtc acaactccaa gggctaccag taacaacagg cgacccagca acttatcaaa
    10621 agattgtaga gcatggactt gattatccag tccccaaggt tgaaccaaga gcgaagcaag
    10681 gaactactga gcgttataaa cctagagtaa gtggccagag gtcaatgact atgaggatta
    10741 ttgacacgct tttcaacgga tttggagacg aaggagggcg caacgtagcg ctgacacgct
    10801 ttgtgggatt actcttcaat aagtgggttg attgtgacct agagaccgcc tatgaactga
    10861 caaagatagc aaatagcgtg acagttgaac cattgcctat tgaagagcta gaccgcagtt
    10921 tcagtagtat agcacgggct gaatatagaa agagaggtta gaaccataga actagaagaa
    10981 ttagaaaacc taaaaagtga aatcatggag gtcagagagc aagaacaccc tcctagaacg
    11041 atgtgggaac tttatagtag gatttgggag cttggggatc aatggcgtaa ggaaaactcc
    11101 tatatcgtca atgaaggaaa gaaaaacgaa agggtagaaa tcccacgacc aagtgttgca
    11161 atcgtagcca aagcgttaca ggaaatttgc cattttacct ttataggtga gggggttata
    11221 agtgatatta gtaagttata tctttaccat ttggatttag gccattatgt atcaagtaac
    11281 gacattttcc gaaaattgct tttaaaatat gactcacgtt tgacttctaa caagttcttt
    11341 ttagaactta tctcctatat ccgtacagaa actaagatga aaccaccgct ggacgattat
    11401 cgatatattc cagtagcgaa tggagtttat aatattaaaa ctcataaact agaggaattt
    11461 agtcctaatt ttgtcattac cagtaagata caaacggaat acaatccatg cgccagaaag
    11521 cctattttag atggttggtt tgattttgat agatggttag aggctttagc agttaacgat
    11581 aaagaagttg tagctttatt gtggcaagtc ataaatgagg ctatcaatcc caaccgaaca
    11641 cgtaagaaaa tggtgctaat ggttggagat ggtaataatg gaaagggtac tttccaatcc
    11701 ttgctggaaa atttaatagg acggtcaaat attagtaatt tgaaacctga ccagtttggg
    11761 aaagagttct atttaggcgc tttgtaagga aaggtttgta atattggaga tgatatttct
    11821 aacaagtacc tggatgaagt ttctgacctt atgagtgtaa ttagtggaga tccagtgcag
    11881 gtcaataaaa aaagcttgca acccgtggaa gcacgtttca gactattgtg tatcttttcg
    11941 ggtaatgatt tgcctagagc tagaaataag accatggggt ggtatagacg gctttgtatc
    12001 attcctttcc gtgcagattt taacggtcag aaagaacgtc atgaaatcaa agaccgattt
    12061 ataaagaaca aagagctgct agagtgggtt ttgtttaaag ttttaaatat gccagatttt
    12121 gatagcttta tagaacctga agcggtacaa aaaatgctta gcaagtacaa aaacgataac
    12181 gattatatca aagtttgggt agagaatttc tacataccaa acgggtggca tgaaataaac
    12241 catgttccaa tgtttattgc aagaaataaa ttaaaagagt ttgctgagga tataggtata
    12301 gataaaccta aacttggaca atttagcaat gcagtaatgg ccgaattgga aaaaacaacg
    12361 tcatataagt actcagccaa aaacggaacg gttagctcta agttttatga cgtacttgac
    12421 ccacaaggtt ttcaacgtaa aagatttgtt aaaggtatat ggggaataca cttagaagat
    12481 gaagatttag gttaggaaat ttaatactaa aaaaccaatt acaccaaggg ttatagcaat
    12541 ttaggttatg caaaaaatag aaacataacc taaacactaa cctttgtaaa cgttgatata
    12601 aaagtctttg cgagtattta ggttagtagg ttagttttta tttctaatat ctctatataa
    12661 aaaataatat atatacgtgt tatataggcg cgaacttaaa aagtaaaact aaccctaatc
    12721 taacaccctt gaaacgttga tatagtaggc ttttgaaggg ttatttttta aacctaatca
    12781 aaacctaatc taagagaaaa aaggagcaaa ccagatggcg atgaacattt gtcaaagagt
    12841 gattagacta gaagaaaccg ttgagcggat cagtgaggcg ctggatggct ttattgagaa
    12901 tgcgaaccat aacgctatag taagcaataa aaacatcgaa cggattcagg aaagttttga
    12961 aatagtcttt aaagagcttg atgaactaaa agcagtacct gacaaacact aaaacattta
    13021 aacaaaatgg ggtagatagt accgaccccc ttgcaatggt gtaactacta gtgacaccct
    13081 aaaaacagaa agaggaaaaa caacatgaca ttaaaaacat tttcagataa agcaaaaaca
    13141 tttaatttca cttacgaatt taaagaccaa gacaccgccc aagtggcagg gagtgcattg
    13201 atgggctaca tgattggaac ttatgaagta ccgtctattt caatcactta caagaataaa
    13261 gggacgctag tagctgagta cgtggaggat aagaaactaa actatatctt taaacgtatt
    13321 tgtgacagtt tcaaagacta ttacaagcaa ccagtgaacg atgaagcatt tgaagaacgt
    13381 tataaacggg agcgcgtgct tcaactcaaa gagtcagagg actttgagag cttgctgaat
    13441 aaggtcacag actacgagct ggaactatta gaatatgctg aacgcttgct ttctgataag
    13501 cctatcctta tggactctat gactgctttt ggtacgttgg aaatgctggg taatgaaagt
    13561 attaacctat tccaaaaatt ggacgtagag ggcgaatata aaggccttgc tgattattct
    13621 ggtcaataaa cacaggggag caatcccctt tttgttatca attcaatagt tttgggtttt
    13681 tccatatagg gggaaataat gaagcggtta gcaatagaaa caatcactaa accaatgaag
    13741 ctgagaggaa tatctaaggg tatagccgag ttggacggtc aaggtttaga gattgatttg
    13801 gataatttag agattgattt tggtggagag tcatttgatt tagcaagaat accaggaact
    13861 aagggtggtt atcgttattt ctttctttgc cctgattgtg gtagacggtg caggttgctt
    13921 tataaacggt atttgtattt tagttgcggt acctgtctag atatccacaa gagcaccctg
    13981 aaccgcagta aaacggattg ccaatattac tgggagcttg cactgaaaga agctagaaag
    14041 gtagaaccag ggtggagtcc tagacgtggt ggatatatgt ttgatggttt ccctgaaaga
    14101 ccaaagtata tgaagcgtga caggtatcat aagcactata agaagttttt gaagtatatt
    14161 gaaaaagggg atagattttg gctgaatggt ttgagattgt aggacgatat gaagtagtgt
    14221 gcaatttaca aaacaaacaa atactacata ttagacggtt tgaatgttaa aaaagctaga
    14281 tatatcaagg atttaagtaa gtagagcgtt ccaaaaaaag ggtgcgataa atggagcgcg
    14341 tgttttgata aaaaaattat tcgtttagga aaaggaaaca ggaaatactt aggatttggt
    14401 taggtagtgg cagtaaatat gcctgacaaa tggcagggtt atgtgtgttg aactccgagc
    14461 gaacgccgag aaagtggcta tatctcctta tcatttatca agcagataag agcaataaaa
    14521 accctcctta actccctgag aaacgacaag gaaataagag agataaaaac atcccttaat
    14581 tgcttatcaa accacaagga gatacggggg ctatctccct ccccacgcac ttctatgagc
    14641 ttcacaccgt cattgtacat tttttctcac ggggaagcat ttgaagctag agcgaacata
    14701 gagaaaacga aaaaagccaa gacactctct gtctcagcta atagttctca caaagactat
    14761 tatatcatac aggaggccga gacatgacac cagagcaggt aaaagaaaca ctagagggcg
    14821 ttaagtggat aaacaaagag atagaaggct tatatttaga gcttgcaact ttagaaagtg
    14881 gtattatcaa aaagcaagaa ctaagcaata ccagagtaca aacaagcagg gtaaatacgg
    14941 cagagaataa tcttataagt gttctgaagc taaaagagga tacgcttcag aggattgagc
    15001 gacttactga ggagagaatg gaaatatcta ggctgatcga taagctggcc aatccgcttg
    15061 agcgttctgt tctcaggctg ttctacttga atgttctcga agcttggcag gttgctgaag
    15121 aaatagatag gtcaaagact acagtatacc ttataaggca aaaagccata gaacacttag
    15181 caaatgtggg gtaataggtg agcacaaaaa aacgcctaga acaatctaag cgctaaagag
    15241 tagtggacgg tgtgcctgtc ccgtcatctc atactatgaa gttgcgtagc gacactatca
    15301 tctctacctc acttctcttt agactattat accacaaagg agcgaactaa tggagctgga
    15361 taagtttaaa acgatgatga acgtcagaga gcggatgacc tactttctac gtttccagag
    15421 gatggcagga agtgaaaacc aagttacgat agatgaagag gcttggaaac ttgtcttacc
    15481 ttatcggtgg gatttgagcg gtgagcatga aaaagcaatc cgtgaggggt tggagatatt
    15541 cgcccaagac atcaacagta tagagaacaa acgagccaga aaatacttta ttatccatta
    15601 ttgttacatg agaaagaaaa caatgagcga atgcgtagaa atggcagcta ctagctccac
    15661 tagctaccac cgatataaac aaatagccgt cttaaatttt gcgagaatcc accagaacgg
    15721 agagctagaa gtgtataagt aggcgtttgt ggtgttttgt tgagaaatgt tgagatttaa
    15781 gtgagggggg atgacatgat tgaagatttt ttgattgatt tggagaatat ccaattagag
    15841 gatatggaga tagattttaa catggatata gacatcgatt taaacataga ttttgaaata
    15901 gatgttgacc ttgatgattt agatttaacc ttactacaag aattagaagc gtaaatgttg
    15961 gcaaatgtag gctgaggctt catattcgtc ccctattttg ctttggtttg gactggacaa
    16021 ttaaaacacc atggataaac tagaagccct cagaaacgag tctaggggct tttgtgaggt
    16081 cataatctta taaaccacgc gcttcatggt ataatatacc tatcagcaac cagcaataaa
    16141 aaaagcacgt ttaaccgtgc tagtttcttg cctgctgaac tcgtcattta ttgccctttt
    16201 gtggggcttt ttttgtggac tttttgagaa tttttaagaa cttttaatga tttctaatca
    16261 aatgagattt ttcaaaaagt cagtaatatc aagggtttag gcgctttaaa caagtttaaa
    16321 agcgtataga ttacaatcta acataaaaag ggataaagtg ctaaccctct aactaagggt
    16381 tctcagtcaa taattttatt catttttcgc tcaaaatcat atcgccccat catgacaaca
    16441 tgctcacaat tactgcattt tattttgata tctgctccta cacgtgtaat ttcccaacga
    16501 ttagcctttt taccagtcga cttgattgta caagcgtgtg attttttcat ctcaacaaaa
    16561 tttccaactt gatacatact actctccttt tctttttcga ttatatcata aaagaggcga
    16621 gccaggctca acctctttca cttaatttgt acgaactggt gtaatgagct gcatgaagtc
    16681 ttcgtcagta tctgctggca caagagtaaa tggacgaaca gctgagataa agctaatggt
    16741 caccttttcg ctatttaaag ctttaagaga atcaatcaag taagttgggt tgaaactaat
    16801 ggtcaaatct tcaccagtaa cctgatcagt atcgatttct tcgtttactt taccaacttc
    16861 tggagagtga acatgggcgc taacaacccc atccttaatt tcaagtttca cagtaccatt
    16921 ttgagtcgca cttgataaaa gacgggcacg ctccattgac tggcgtaagt ttaccacatt
    16981 aaaagtaata gtagtgttaa agtctgttgg aatcaagcga tctgtatcag gatagtttcc
    17041 ttctaggaga cgagtataga agctaatatt ttcgcttcta aagaggattt ggttattggc
    17101 aaagaaaatc tctacagttt cgatatcatc tgtaaatacc gctgaaaatt cgcgtagaga
    17161 acggctagga attacgacat caaaatcatc actatttttt tcaagagtca attttttctg
    17221 gcttaggcga tgagagtctg ttgcaactgt ttttaactct ttgtattgac tcaatacgaa
    17281 gtggacacct gttaaaatcg gacgactctc ttgtgtactt gcagcaaagg ctgtttcatt
    17341 aataattttc ttgagtaatt ttgtttcaag tattaaagga gtgcttgctg aaatttcttg
    17401 gattcgtgga tattgttcgc tatcttttcc ttttagggta atttctgatt tgccactggt
    17461 taaaacaatt tgattttgtt caatttcttt aaaatcaaga gttacatcag gtaaactaga
    17521 tactacattg ataaagaaag aagcttcaag aaggatcgaa cctaaagaag taattaacaa
    17581 accagcatct tcattttttt gagaaataaa attttcaatt gaaatttgac catttgaacc
    17641 aattaaagta ataccttcat tggtcacgtc aatttttact gttgataaaa taggaatggc
    17701 atttttagaa cta
//
LOCUS       DAVGJL010000028        17651 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.028, whole genome shotgun sequence.
ACCESSION   DAVGJL010000028 DAVGJL010000000
VERSION     DAVGJL010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17651
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.028"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            124..255
                     /locus_tag="VVE42_000832"
     CDS             124..255
                     /locus_tag="VVE42_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEU3073048.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            276..1826
                     /gene="dltA"
                     /locus_tag="VVE42_000833"
     CDS             276..1826
                     /gene="dltA"
                     /locus_tag="VVE42_000833"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEU3073049.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQEHTYGDLKADSDS
                     LAAVIDQLGLPEKSPVVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPSLIIAISAFPLEQVFTPMINLAQVQEAFAQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMITDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWTSTPSFADMAMLSEYFNSEKMPGITHFYFDG
                     EELTVKTAQKLRERFPNARIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMKNPEKTAEAFFEFEGLPAYHTGDVGT
                     MTDEGLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDGVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            1823..3067
                     /gene="dltB"
                     /locus_tag="VVE42_000834"
     CDS             1823..3067
                     /gene="dltB"
                     /locus_tag="VVE42_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEU3073050.1"
                     /translation="MMEFFQQLPHLEPYGNPQYFVYVIAATLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINETQSLLGFLGISYLTFRSVGIIIELRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQAIPERDELMDMLDESVRYIMRGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQLLGMVVT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            3081..3320
                     /gene="dltC"
                     /locus_tag="VVE42_000835"
     CDS             3081..3320
                     /gene="dltC"
                     /locus_tag="VVE42_000835"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEU3073051.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDIRVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            3313..4581
                     /gene="dltD"
                     /locus_tag="VVE42_000836"
     CDS             3313..4581
                     /gene="dltD"
                     /locus_tag="VVE42_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEU3073052.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMHHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFVPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLQQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKEELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMDYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            4736..5176
                     /gene="adcR"
                     /locus_tag="VVE42_000837"
     CDS             4736..5176
                     /gene="adcR"
                     /locus_tag="VVE42_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HEU3073053.1"
                     /translation="MRQLAKDINAFLNEMILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            5176..5880
                     /locus_tag="VVE42_000838"
     CDS             5176..5880
                     /locus_tag="VVE42_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEU3073054.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYCVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGKKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNAHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVGHA"
     gene            5873..6679
                     /locus_tag="VVE42_000839"
     CDS             5873..6679
                     /locus_tag="VVE42_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEU3073055.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGKSSSSMSLDQYLFGSIVTISEEQVISLFVIAAVVLILTFLFLRPMYILTFD
                     EDTAFVEGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            6689..8194
                     /gene="adcA"
                     /locus_tag="VVE42_000840"
     CDS             6689..8194
                     /gene="adcA"
                     /locus_tag="VVE42_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HEU3073056.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVAKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGEEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKETFEKNAAAYIEKLQALDKAYAEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDAEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISVMEKNLKALKQTTDQEGPAIEPEKAEDTKTVQNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYQTDVTK
                     INITDNTMEFVQGGQSKKYTYKYVGKKILTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNVAPVKAEHFHIFFGGTSQEALFEEMDNWPTYYPDNLSGQEIAQEMLAH"
     gene            complement(9322..10095)
                     /locus_tag="VVE42_000841"
     CDS             complement(9322..10095)
                     /locus_tag="VVE42_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU3073057.1"
                     /translation="MIKDERVLELIEIIKKKKRIAVKELAEITFSSTSTLRRDLIFLE
                     NQGLIKRKHGYVTLSSMNTIELSHQIREGESTRQKRLIASLAKDFIRSGMCIYLDSST
                     TVYELCPYLSELDNLIIFTNGLHTAQTLSETVKDSSKIFITSGEVKHQSCSVVNYDKE
                     NSLLDHFNIDLAFCSARGIDDQYVYEASLSQAISKKNIIDKAHETILLIDSSKFYKTG
                     FFKINPLSKYTTFISDTVPDQKLLDAVELFDGEWVSDIQ"
     gene            10271..11674
                     /locus_tag="VVE42_000842"
     CDS             10271..11674
                     /locus_tag="VVE42_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEU3073058.1"
                     /translation="MEAVLAIDLGATSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDIDFLLAKILESIRLANTSYKILSIGIDTWGVDFGLIDNEGKLLSQPVHYRDERT
                     KGVLKEISEMTELEKLYSETGNQIMEINTLFQLFKARQESPDSFYKTNKILLMPDLFN
                     YLLTGKFATEKSIASTTQLFDPRSQNWNQNILKLFELDSSLLPEIVSEGNVLGRIKKE
                     YGLGDIPVVNVCSHDTASAIVSVPKTEGSLFISSGTWSLVGVELTSPLLTTESFSYGF
                     TNEVGKDGVITFLKNCTGLWIIEELRRSFERRGKAYSFDDIRTMVEKEKENLPLIDTE
                     STEFATESDMHKTLTEYLAYHHETREWTDGQLFKIVYESLAETYRKAIELLEELTHKV
                     YKRIYVIGGGARASYFNQMIADRTGKEVLTGSTEATAVGNIVVQLIAMGQLKGMEEAH
                     HVIEEFLQLESYYSQKN"
     gene            11789..12427
                     /locus_tag="VVE42_000843"
     CDS             11789..12427
                     /locus_tag="VVE42_000843"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HEU3073059.1"
                     /translation="MSDVKQELIKYGKKLVETDLTKGTGGNLSVFDREKQLMAITPSG
                     IDFFEIKESDIVVMDINGNVVEGERLPSSEWYMHLIQYQTRDDIDAIIHAHTTYATVL
                     ACLREPLPASHYMIAVAGKDVRVAEYATYGTKELAVNAAKAMEGRRAVLLANHGILAG
                     AQNLLNAFNIVEEVEYCAKIYCLAKNFGEPVVLPDEEMELMAEKFKTYGQRK"
     gene            12439..12879
                     /locus_tag="VVE42_000844"
     CDS             12439..12879
                     /locus_tag="VVE42_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HEU3073060.1"
                     /translation="MLKHIPKNISPDLLKTLMEMGHGDEIVLADANYPSASCANKLIR
                     CDGVNIPELLDSILYLMPLDSYVDSSIQFMNVVSGDDIPKIWGTYRQMIEGHGTDLKT
                     ITYLRREDFYERSKKAYAIVATGETSLYANIILKKGVVVERENV"
     gene            12872..13303
                     /locus_tag="VVE42_000845"
     CDS             12872..13303
                     /locus_tag="VVE42_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU3073061.1"
                     /translation="MFNRGILVASHGNFASGALMTAEMFVGETTNDRVRTLGLMPGEN
                     IVEFEHYFKNQVNELLDSNQEVIVLTDLIGGSPNNVALSRFLNLDSVDIVTGFNIPLL
                     VELISSYDSKINLEEIVQNAQNSLFNVKQQLNVEEEEDLCL"
     gene            13294..13764
                     /locus_tag="VVE42_000846"
     CDS             13294..13764
                     /locus_tag="VVE42_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU3073062.1"
                     /translation="MSIEFVRIDDRLVHGQVVTTWLKKYDIEQVIIVNDRISEDKTRQ
                     SILKISAPVGLKIVFFSVKRFVEVLNSVPIKKRTMLIYTNPKDVYDSIEGNLKLEYLN
                     VGQMSKTEENEKVTGGVALGEEDKYYFKKIVDKGTRVEIQMVPNDKVTMLEKFL"
     gene            13789..14562
                     /locus_tag="VVE42_000847"
     CDS             13789..14562
                     /locus_tag="VVE42_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEU3073063.1"
                     /translation="MLFTQALLVTLVGIIATIDYNGPLFMIHRPLVTSAMVGLVLGDF
                     TQGVLIGSALELTWLGVTGIGGYTPPDTISGAIIGTAFGILSGQGETAGIAIAVPIAV
                     ATQQLDVLAKTLDVYFVKKADNDAKNGDYSKIGFYHYSSLVLITLFKIVPIFLAIMLG
                     GEYVADLFAKVPPIVMQGLNSAGALLPSIGFGMLLNMMLKKNMWVFLLIGFICSVYGG
                     MSTIGISLVGIAVAYFYDMIGSKPQETTSSSDVEEDLDL"
     gene            14559..15359
                     /locus_tag="VVE42_000848"
     CDS             14559..15359
                     /locus_tag="VVE42_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU3073064.1"
                     /translation="MMNNKVTKVELKKVFKRSFMYGSSWNYERMQNLGFLYTILPVLK
                     KLYPDKDSASPAMKRHLEFFNTHQTAAPFILGVTSAMEEQEGNEGAASITGIKVGLMG
                     PLAGLGDSLFWLTLVPICFSIGASYSKDGGALGIFIALILFNIINIPVKYFGLKYGYT
                     KGSSLIQENNTKGTLNRVTSMATALGLVLVGGLIPSMVGINFGLEFKQGELVISVQEM
                     ITKLIPGFIPMALTLLMCKLIRKGKNPVVLIFSVMAIGVILVVLGILK"
     gene            15418..>17651
                     /locus_tag="VVE42_000849"
     CDS             15418..>17651
                     /locus_tag="VVE42_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEU3073065.1"
                     /translation="MKLWYKKAAANWNEALPIGNGHLGGMIYGSATKECIQLNDETIW
                     YRGKSDRNNPDSLLHLKKIREYLLDGEIQKAEELIKLTMFATPRDQSHYELLGELYIE
                     HIDIQSCALSLYERELDLDTAISNVVFEPNSCNLQIKREYFTSFNKNILCCRIVSSVQ
                     NTLNLNINLGRNKRFNDEVSKLDSSTILMSASAGGRKGVQFKVVCHSKVTDGEVSVLG
                     ETIVIRNATEVFLYLKSMTDYWGNIDISSLQGEFSSIDYFTEKDEHVKKYQEQFNRVD
                     FKLDYSKDCLSIPTNLLLENTKKYSNYLTNLLFHYGRYLLISSSQPNGLPANLQGIWC
                     DELNPIWGSKYTININTQMNYWMVGPCDLPEVEYPLFDMLERMREPGRLTAKKMYGAR
                     GFTAHHNTDGFGDTAPQSHAMGAAIWVLTIPWLCTHIWEHYLYFQDERILTEHFEMIK
                     EAFLFFEDYLFEVDGYLMTGPSVSPENKYRLKNGIEGNACLSSTIDNQILRYFCDSCI
                     GIAKQLGDNSDFISRVKELKKKLPRTKIGSNGQIQEWLEDYEEVEPGHRHISPLFGLY
                     PYNEIDIHKTPELAEAAKITINRRLSNANFLSSQDREQAINNWLVSGLHASTQTGWSA
                     AWLIHFFARLYQGEPAYNQINGLLNNATLGNLFLDHPPFQIDGNLGLVSGICELLVQS
                     HHNWLSLIPALPSAWSEGEVKGFRVRGGYKVSFAWKNGDITFLKLEGGNKDQKVRVRI
                     YGKNT"
BASE COUNT         5444 a         2942 c         3788 g         5477 t
ORIGIN      
        1 attggaaaca tacggtaaac aattgaaaag atactatata aagtggtaca atggtagaaa
       61 aataagctag taagaatcac tcttatttta gcgaaaaatt acagaaatga atggtttttc
      121 ttgatgaaac agagaaaaga attgtacctc tttcttggtc ggacagcctt gtattttctt
      181 atctttctag ggctgcttta cttctttagc tatcttggtc agggtcaagg aagctttatc
      241 tataatgaat tttaacttga aggagaatcc ctattgtgtc aaataaacca atagcagata
      301 tgattgaaac cattgagcat tttgctcaga cacagcctag ctatcctgtc tataatgttt
      361 tggggcagga acacacttat ggcgatttaa aggctgattc ggatagtttg gctgcagtca
      421 ttgaccaact aggcttgcct gagaagtctc ctgtggttgt ttttggtggc caagaatatg
      481 aaatgttggc aacctttgta gcgctgacta agtcaggtca tgcctacatt ccaattgata
      541 gccattcggc cttggagcga gtttcagcta ttttagaagt agcagagcca agcttgatta
      601 ttgccatttc agcctttccc ttggagcagg tttttacacc aatgataaat ctagctcagg
      661 ttcaagaagc ctttgcccaa gggaataact atgaaatcac gcatccagtc aagggagatg
      721 ataattacta cattatcttt acttctggta cgactggtaa gcctaaggga gtgcagattt
      781 cacatgataa tctcctcagc tttacaaact ggatgattac ggataaggaa tttgcgacac
      841 cgagtcgtcc gcaaatgctg gcacagccac cttattcttt tgacttgtct gtcatgtatt
      901 gggcaccgac cttggcactt ggtggtacgc ttttcactct tccttcagtc atcactcagg
      961 actttaagca actctttgcg gctatctttt cattgccaat cgctatctgg acatcaacac
     1021 catcctttgc agatatggcc atgttgtctg aatacttcaa cagtgagaaa atgcctggaa
     1081 tcacgcattt ctactttgat ggtgaagaat tgacggtcaa aacagctcaa aaactgcgcg
     1141 agcgtttccc aaatgcccgt atcatcaatg cttacggccc aacagaagcg acagtagctc
     1201 tgtcagcagt tgccgtgaca gacgagatgt tagcgactct caaacgccta ccaatcggct
     1261 ataccaaggc tgattctcca acctttatca ttgacgagga aggaaataaa ctgccaaatg
     1321 gcgaacaggg agaaatcatt gtttctgggc cagctgtttc aaaaggttat atgaaaaatc
     1381 ctgaaaaaac agcagaagcc ttctttgagt ttgaaggtct gccagcctat cacacaggcg
     1441 atgtgggaac catgacagat gagggcttgc ttctctacgg cggacgcatg gacttccaga
     1501 ttaagtttaa cggttaccgc attgagttag aagatgtctc tcaaaacctc aacaagtctc
     1561 gctttatcga atctgctgtc gcagtaccgc gctataacaa ggaccacaag gtacaaaatc
     1621 tattggctta tgtcatctta aaagacggtg ttcgtgagca gtttgagcga gatatcgata
     1681 ttaccaaggc catcaaggaa gacctgacag acatcatgat gtcctatatg atgccatcta
     1741 aattccttta ccgagacagt ttgccactaa ctccaaatgg aaagattgac atcaaaggat
     1801 tgattaacga ggtgaataag agatgatgga gttttttcaa cagcttcctc atttagagcc
     1861 atatggcaat cctcagtatt ttgtttatgt gattgctgca accttgccca tctttatagg
     1921 cctctttttc aagaaacgct ttgcctggta tgaagtgttg gtaagtctct tctttattgt
     1981 caccatgttg gtgggtggaa agaccaatca actagctgcc ttgggtattt acctttgctg
     2041 ggaaatattg ctcctgcttt tctacaagca ttatcgaaaa agcaaggatg gcaagtgggt
     2101 cttctactta gttagttttc tgtctctact tccgattatc tttgtcaagg tgcaaccagc
     2161 tatcaatgaa acgcagtctt tgcttgggtt cttgggaatt tcttacctga cctttcgttc
     2221 ggttgggatt atcatcgagc tgagagatgg agtgattaag gattttaccc tctgggaatt
     2281 cctccgtttc cttctcttca tgccaacttt ctcgagtggt ccaatcgatc gctttaagcg
     2341 atttaatgaa aattatcagg ccattcctga gcgagatgag ttgatggata tgctggatga
     2401 atctgtccgc tatatcatgc ggggcttttt gtataagttt atcctagctc atgttttagg
     2461 agagacctta ctacctcctc tgaagaattt agccttgcag tcaggtggct tctttaatct
     2521 ctatgccttg gcagttatgt atacttttgg tctggagctc ttctttgact ttgcaggtta
     2581 ttctatgttt gctttggcca tctcaaactt gatgggaatc cgtagcccta tcaactttaa
     2641 caagcccttt ttatcaaggg atttaaagga gttttggaat cgctggcata tgagtctgtc
     2701 cttctggttc cgtgactttg tctttatgcg aatggtgatg gtgttaacca gaaagaaagt
     2761 ctttaaaaat cgtaatgtaa cctcaagcat ggcctacatt gtaaatatgc tgattatggg
     2821 attttggcat ggtgtgacct ggtactatat cgcctatgga ctctttcatg gactaggctt
     2881 ggtcatcaat gatgcctggg ttcgcaagaa aaaaacgctc aataaggaac ggaaaaaagc
     2941 agggaaggct gccctacctg agaatcgctg gattcagttg cttggcatgg ttgtcacttt
     3001 ccatgttgtc atgttgtcat tcttaatctt ttctggattc ttgaataatc tatggtttaa
     3061 aaaataaaag gaaataaaat atggatatca aatcagaagt tattgaaatt attgatgagt
     3121 tgtttatgga agatgtttct gacatgatgg atgaagatct ttttgatgca ggtgtcttgg
     3181 atagtatggg aacggttgag ttgattgtgg agattgagaa ccgttttgac attcgtgtcc
     3241 ctgtaacaga gtttggtcgt gacgactgga atacagctaa taaaattata gctggtattg
     3301 tggagctaca aaatgcttaa acgcttatgg atgatcttcg gaccggtctt gatcgctggt
     3361 ttgttggttt ttctgctcat tttcttttat cctactgaga tgcatcataa tctaggagct
     3421 gaaaagcgtt cagcggtggc tactactatc gatagtttta aggagcgaag tcaaaaagtc
     3481 agagcactat ctgatccaaa tgtgcgtttt gttcccttct ttggctctag tgaatggctt
     3541 cgttttgacg gtgctcatcc tgcggtatta gctgagaaat acaatcgttc ctaccgtcct
     3601 tatcttttag gacagggggg agctgcatcg cttaaccaat attttggaat gcaacagatg
     3661 ttacaacagc tggagaataa acaagttgtg tatgttatct cacctcagtg gttcagtaaa
     3721 aatggctatg atccagcagc cttccagcag tattttaatg gagaccagtt gactagtttt
     3781 ctgaaacatc aatctgggga tcaggctagt caatatgcag cgactcgctt actgcaacag
     3841 ttcccaaacg tagctatgaa ggacctggtt cagaagttgg caagtaaaga agaattgtcg
     3901 acagcagaca atgaaatgat tgaattattg gctcgtttta atgaacgcca ggcctccttt
     3961 tttggtcagt tttcggttag aggctatgtt aactacgata agcatgtagc taagtattta
     4021 aaaatcttgc cagaccagtt ttcttatcaa gcaatagaag atgttgtcaa agcagatgct
     4081 gaaaaaaata cttccaataa tgagatggga atggaaaatt atttctataa tgagcagatc
     4141 aagaaggatt tgaagaaatt aaaggattct cagaaaagct ttacctatct caagtcgcca
     4201 gagtataatg acttgcagtt ggttttaaca cagttttcta aatctaaggt aaacccgatt
     4261 tttatcattc cacctgttaa taaaaaatgg atggactatg ctggtctacg agaggatatg
     4321 taccaacaaa cggtgcagaa gattcgctac cagttagaaa gtcaaggttt taccaatata
     4381 gcagattttt ctaaggacgg cggggagcct ttctttatga aagacaccat tcaccttggt
     4441 tggttgggtt ggttggcttt tgacaaggca gttgatcctt tcctatccaa tcccacacca
     4501 gctccgactt accatctgaa tgagcgcttt ttcagcaaag attgggcgac ttatgatgga
     4561 gatgtcaaag aatttcaata gataatataa tatagaagag ttcaagaatg aaactagttt
     4621 tcacgttttt tcttgactct ttttttgctt gtgatataat taactggtaa acaaaatgtc
     4681 aaagaatagt atttttctct taaaaaagag aagtctaaaa ggaaaggagt cagatatgag
     4741 acagctagca aaggatatca atgctttttt gaatgagatg attttgcagg cggaaaatca
     4801 gcatgaaatc ctaataggtc attgcactag cgaggtggcc ctgaccaata ctcaggagca
     4861 tatccttatg ctcttgtcag aggaatcttt aacaaattca gaattggccc gtcgtctcaa
     4921 tgtcagtcag gcggcagtta ccaaggccat taagtctttg gtcaaggaag ggatgttgga
     4981 aacatctaaa gattctaaag atgcgcgtgt gattttttat cagttgactg acttggctcg
     5041 tccaatcgct gaggagcacc accatcacca tgagcataca cttttaacct atgaacaagt
     5101 ggcgactcag tttactccaa atgaacaaaa agtgattcag cggtttttga ctgctttagt
     5161 aggagaaatc aaataatgag atatattacg gtagaggatt tgtccttcta ttatgataag
     5221 gagcctgttc ttgaacatat caattattgt gttgatagtg gggaatttgt taccttgact
     5281 ggggaaaatg gagcggctaa gacgacgctc atcaaggcta gtcttggaat tctgcaacca
     5341 cgcattggaa aggtggctat ttcaaagaca aatacgcaag gtaagaaatt gagaatagcc
     5401 tatcttcctc aacaaattgc cagttttaat gctggttttc caagtacggt ctatgaattt
     5461 gtcaagtcgg gtcgctatcc gagaaaaggc tggttccgtc gtttgaatgc tcatgatgag
     5521 gagcatatca aggctagtct ggactcagtt ggcatgtggg aacatcgaga caaacgcttg
     5581 gggtctctat ctgggggaca aaagcagcga gcggtaattg cgcgtatgtt tgcttctgac
     5641 cctgatgtgt ttatcctaga cgagccgaca acggggatgg atgcaggaag taaaaatgaa
     5701 ttttacgaac tcatgcacca cagcgcccat catcatggta aggctgtttt gatgattacc
     5761 catgaccctg aagaagttaa ggattatgcg gatcgcaata ttcatctagt ccgtaaccaa
     5821 gactcgccat ggcgttgttt caatgttcat gagaatggcc aggaggtggg ccatgcttag
     5881 tttattatct tacgacttta tacagcgcgc ctttttggcg gttattgcta tgagtctttt
     5941 ctcaccggta ttgggaacct tcctcatctt gcgtcgtcag agtttgatga gtgataccct
     6001 tagccacgtc tcactttcag gtgtagcctt tggtctggtt ttggggattt ctccaactgt
     6061 ttctactatt gccattgtct tgattgcggc ggtctttctg gagtatctcc gtacggttta
     6121 caagagcttt atggaaatcg ggacagctat cctcatgtca acaggtctgg ctgtttctct
     6181 gattgtcatg agcaagggta aaagctcgag ttcaatgagt ttggaccaat atctctttgg
     6241 ttcgatcgtg actatcagtg aagaacaggt catttccctc tttgtcattg cggcggttgt
     6301 tttgattttg acctttctct ttcttcgtcc tatgtatatc ttaacttttg acgaagatac
     6361 ggcctttgtg gaaggcttgc cagttcgtac catgtccatt ctttttaaca tggtgacagg
     6421 ggtggctatt gcccttatga ttcctgcagc aggagctctt ctggtatcga ccattatggt
     6481 cttgccagct agtattgccc tgcgtctggg gaaaaacttt aaatcggtta tgctgcttgc
     6541 cagtgcgatt ggctttttgg gaatggtagc aggactttac atttcttact atgcagaaac
     6601 acctgcaagt gcaagtatta ccattatttt tgtaactgtc tttatactaa tcagtttagt
     6661 aagacgtttt atcaaatagg agacgaacgt gaaaaaaatt agcttattac tagccagtct
     6721 atgtgctttg tttttagtgg cttgttccaa tcaaaaacag gcagatggta aactcaatat
     6781 cgtgacaacc ttttaccctg tctatgaatt taccaagcaa gtcgcaggag atacggctaa
     6841 tgtagaactc ctaatcggtg ctgggacaga acctcatgaa tacgaaccat ctgccaaggc
     6901 agttgccaaa atccaagatg cagatacctt cgtttatgaa aatgaaaaca tggaaacatg
     6961 ggtacctaaa ttgctagata ccttggataa gaaaaaagtg aaaaccatca aggcgacagg
     7021 cgatatgttg ctcttgccag gtggcgagga agaagaggga gaccatgacc atggagaaga
     7081 aggtcatcac catgagtttg acccccatgt ttggttatca ccagttcgtg ccattaaact
     7141 agtagagcac attcgtgaca gcttgtcagc agattatcct gataaaaaag agacctttga
     7201 gaagaatgca gctgcctata tcgaaaaatt gcaagccttg gataaggctt acgcagaagg
     7261 cttgtctcaa gcaaaacaaa agagctttgt gactcaacac gcagccttta actatcttgc
     7321 cttggactat ggactcaaac aagtcgcaat ctcaggactt tctccagatg cagagccatc
     7381 agctgctcgc ttggcagaat tgacagagta tgtcaagaaa aataaaatcg cctatatcta
     7441 ttttgaagaa aatgcctcac aagcccttgc taacacactt tcaaaagaag caggtgtcaa
     7501 aactgatgtc ctcaatcctt tagaaagtct gacagaagag gacaccaagg ctggagaaaa
     7561 ctacatttcc gtgatggaga aaaacctcaa ggctttgaaa caaacaacag accaagaagg
     7621 cccagcaatc gaacctgaaa aggcagagga taccaagaca gtccaaaatg gttacttcga
     7681 ggatgcagct gtcaaggacc gtaccttgag tgactatgca ggtaactggc aatcagttta
     7741 tcctttcctt gaagacggca cgtttgacca agtctttgac tacaaggcta agttgactgg
     7801 taagatgacc caggctgagt acaaggctta ctatacaaaa ggctatcaga cagatgtgac
     7861 taagattaac attactgata atactatgga atttgttcaa ggtggacaaa gcaagaaata
     7921 cacttacaag tatgtcggta agaaaatttt gacttacaag aaaggcaatc gtggcgtgcg
     7981 tttcctcttt gaagccacag atgctgacgc tggacaattc aaatatgttc agtttagtga
     8041 ccacaatgtt gccccagtta aggcagaaca tttccatatc ttctttggag gcacaagcca
     8101 agaagccctc tttgaagaaa tggacaactg gccaacctac tacccagata acctatctgg
     8161 ccaagaaatc gcccaagaaa tgttggcgca ttgatgagaa atcgactagt tcataagagc
     8221 tagtcggttt tttgatacta gatggctaac ttcattatag acctttcaga actaagggaa
     8281 cctgaaaaat aaataaaact cttgaaatat ctgggcttat atgctagaat gtagttagtg
     8341 tataaggttc attagaacac caaagcccct aactagtgcc atagttaagg gcttttttga
     8401 cgtatcttag cgatttaacg ggtgtttata ggctagtcag tcggtctact tcttaccatc
     8461 tagccatttg cagacgaaat acaagataat tccagctata acatctgtta taatagtaag
     8521 agcaagctct ggtataaggc tcattagttt cacctcctct cacgaaccca taggaacgta
     8581 atcggtaacc gatgacaaaa atagtatacc acactaaatt tagatcatca acctcactta
     8641 atgcttgaac tattgtagat tatatgctaa aatgtagtta attgataagg ttcattagaa
     8701 caccaaagcc cctaactatg gccgtagtta agggcttttt tgacgtatct tagcgattta
     8761 acgggtgttt ataggctagt cagtcggtct actccttact atctagccat ctgcagacga
     8821 aatacaagat aattccagct ataacatccg ctataatagt aagagcgagc tctatgataa
     8881 ggctcattag tttcacctcc tctcacgaac ccataggaac gtaatcggta accgatgaca
     8941 aaaatagtat accacaatac atttagatca tcaaggtcac ttaattcttg aaatataggg
     9001 tctaggcgac aaagtcttta aaatcagaaa aacgcataat atcaggtgtg caaaaacttg
     9061 atactatgcg ttttattgtg ggaaggttta ctccattttc tcctgaaatt gagtttttgt
     9121 ccagcctctg tttttagaat tgctaagaaa ataatgtcat gtggtgaata tttgtaaatc
     9181 agtcagcaga cagaacgaat actcttcgaa aatctcttca catcatgtca gcttcgtctt
     9241 gccgtgtcca tgggggactt gacttcatca gttttcaaat ctgtactgtg agcaagttga
     9301 gactagcttc ctatttgatt ttcattgaat atcagaaacc cattctccat caaataattc
     9361 gactgcgtct aataattttt gatctggcac ggtgtctgaa ataaaggttg tgtatttgga
     9421 gaggggatta attttaaaaa atccagtctt gtaaaattta gaactatcaa tcagtaagat
     9481 ggtttcatgg gctttgtcaa taatattctt ttttgaaata gcttggctga gagaagcttc
     9541 ataaacatat tggtcatcaa tacctcttgc tgaacaaaat gctaaatcga tattaaaatg
     9601 atctaataaa gaattttcct tatcatagtt gaccacggaa caggattgat gtttgacctc
     9661 gccagatgtg ataaagattt tggagctatc tttaacagtt tcagataggg tttgtgcagt
     9721 atgtaaacca tttgtaaaaa taatcaaatt atcaagttca gaaagatagg gacagagttc
     9781 gtagacagta gtactggaat ccagatagat acacatacca gaccgaataa agtctttagc
     9841 gagactagcg attagtcttt tttgcctagt actttctcct tcacgtattt gatgagaaag
     9901 ttcaattgtg ttcatagagg acagggtcac gtatccgtgc tttcttttga taagaccttg
     9961 attttctaag aaaattaaat cacgacgtaa ggtacttgtg ctggagaaag tgatttctgc
    10021 cagctctttt acggcaattc tttttttctt tttgataatt tcaatcaatt caagtacacg
    10081 ttcatctttt atcataagct cctcctaatt tatcatttca actatattat agcacaaatt
    10141 ggaggaattt gaattatttt tatgaatatt gggttaacat ttgaacatta ttcaagtaag
    10201 cgttcacata ttgaaaaaat aaaacgtggg gattataata aagttaatca aggacgaaga
    10261 gagaagaaaa atggaagcgg ttttagcaat agatttaggt gcgacttctg gaagagcaat
    10321 cgttggttac ctttctgaaa ataaactagt aatggaagaa ataaatcgct tttctaatct
    10381 acctattaga gtaaaagggc atttatcttg ggatattgac tttctactag ctaaaattct
    10441 tgaaagtatc cgcttggcta atactagtta caagatttta tctatcggta ttgacacatg
    10501 gggagttgat tttggactga ttgataatga aggtaagctg ttatcacaac ctgttcatta
    10561 tcgtgatgaa agaacaaagg gagtgttaaa ggaaatatct gaaatgactg aattagaaaa
    10621 actgtattca gagacaggaa atcagattat ggagataaat accttgtttc aactctttaa
    10681 ggcacgtcaa gaatctcctg actctttcta taagaccaat aagattcttt taatgccaga
    10741 tttgtttaat tatctcttga caggtaagtt tgctacagaa aaaagcattg cttcaacaac
    10801 tcaattattt gatcctagga gtcaaaactg gaatcagaat atcttaaaac tatttgaatt
    10861 ggattcatct ttacttcctg agattgtttc agagggaaat gttcttggaa ggataaaaaa
    10921 ggagtatggt ttaggcgata ttcctgttgt gaatgtttgt agtcatgata cagcaagcgc
    10981 gattgtctca gtacctaaga cagaaggtag tttatttatt tcatcaggta cttggtcttt
    11041 ggttggagtg gaacttactt caccgcttct tactaccgaa tccttcagtt atggatttac
    11101 aaatgaagtc ggtaaagatg gagtgattac atttctgaag aattgtacag ggttgtggat
    11161 catagaggaa ctaagacgtt catttgaacg aagagggaaa gcctattctt ttgatgatat
    11221 taggacaatg gtggagaaag aaaaagaaaa tcttcctctg attgatactg aatcaactga
    11281 atttgcaaca gaatctgata tgcacaagac tttgacagaa tatctagctt atcatcatga
    11341 aactagagag tggacagatg gacaactatt taagattgtt tatgaaagcc tagctgaaac
    11401 gtataggaaa gcgatagagt tactagaaga actaactcat aaggtttata agaggatata
    11461 tgtgattgga ggaggtgcta gagccagtta ttttaaccaa atgattgctg atagaactgg
    11521 taaagaggtt cttacaggtt cgactgaggc tacagctgtg gggaatattg ttgtgcagct
    11581 catagctatg ggacaattaa aagggatgga agaggctcac catgttattg aggagtttct
    11641 acaattagag agttattact cccaaaagaa ttaaaaagat tgagagtttg taaatttgcc
    11701 tccctccccc ttcttagctt ttgtgcagga aggggggata attggtgaat tgaaaaatat
    11761 ttagtgtttt gatatgagga ggacaaggat gtcagatgta aaacaagaat taattaaata
    11821 tggtaagaag ctagtagaaa cagatttgac gaaaggaaca ggtgggaatc tcagcgtttt
    11881 cgatcgtgaa aaacaattga tggcaattac cccgtcgggt attgatttct ttgaaatcaa
    11941 agaatctgat attgtagtga tggatattaa tggaaatgtt gtagagggag aacgcttgcc
    12001 atctagcgaa tggtatatgc atttgattca atatcaaact cgtgatgata tcgatgcaat
    12061 tatccatgct catacaactt atgcaacagt attagcttgt ctcagagaac cacttccagc
    12121 gagtcattat atgattgcag tggcagggaa agatgttcgg gtagctgagt atgcaacata
    12181 tggtacgaaa gaattggctg tgaatgcagc taaagcaatg gaaggtcgta gagcagtttt
    12241 actagcgaat catggaattt tagcaggtgc acaaaattta ttgaatgcat ttaatattgt
    12301 tgaagaagtt gaatattgtg caaaaattta ttgtttagct aagaattttg gagagccagt
    12361 agttcttcct gatgaggaga tggaattgat ggcagaaaaa tttaaaacat acggtcagag
    12421 aaaataggga ggatattaat gttaaaacat ataccgaaaa atatttctcc agatttattg
    12481 aagactttaa tggaaatggg acatggagat gaaatagtat tagctgacgc gaattatcct
    12541 tctgcctcat gtgcaaataa gctaattcgt tgtgatggtg taaatattcc agaattatta
    12601 gattccattc tgtatttaat gccattagat agttacgtcg atagttcaat tcagtttatg
    12661 aacgttgttt cgggtgatga tattcctaag atatggggta cctatagaca gatgattgaa
    12721 ggtcatggta cagatcttaa aacgattact tatcttagaa gagaagactt ttatgaacgt
    12781 agtaagaaag cttatgctat tgttgctaca ggagaaactt cactttatgc taatattatc
    12841 cttaagaaag gagtagttgt tgaaagagaa aatgtttaat agaggaattt tagttgccag
    12901 tcatggtaat tttgctagcg gagctctcat gaccgcagaa atgtttgttg gtgagacaac
    12961 aaatgataga gttaggacat taggtttgat gcctggagag aatattgtag agtttgaaca
    13021 ttattttaaa aatcaagtaa atgaactgtt agactcaaat caagaggtta tcgttttgac
    13081 tgacttgatt ggaggaagtc ctaataatgt ggctttgtca cggtttttaa atttggattc
    13141 agttgatatt gtaacagggt ttaatatccc tctcctagtg gaattaatat caagttatga
    13201 ttcaaaaatc aatttagaag aaattgttca aaatgctcaa aatagtttgt ttaatgttaa
    13261 acaacaactt aacgtagagg aggaagagga tttatgtcta tagagtttgt tcgtattgat
    13321 gaccgtctgg tacatggtca agttgtcact acgtggctaa aaaagtatga tattgagcaa
    13381 gttatcattg ttaatgatcg catctcagaa gataaaacac gacaatctat tttaaagatt
    13441 tctgcaccgg taggtttaaa aattgttttc tttagtgtaa aacggtttgt ggaagtttta
    13501 aactctgtgc caataaaaaa gagaacaatg ctgatatata caaatccaaa agatgtgtat
    13561 gattctattg aaggaaattt aaaattggag tacctcaatg taggacagat gagtaaaacg
    13621 gaggaaaatg aaaaggtaac gggaggtgta gctctaggtg aagaagacaa atattatttt
    13681 aagaaaatag ttgataaggg aacgagagtt gaaattcaaa tggttcctaa tgataaagtt
    13741 acaatgttgg aaaaattttt ataaaaataa tttaaggagg tacagtatat gctattcaca
    13801 caagcattac tggtgacatt agttgggatt attgccacta ttgactataa tggaccgtta
    13861 tttatgattc accgtccgtt agttacaagt gcaatggttg gcttagtctt aggagatttc
    13921 acccaaggtg ttcttattgg ttcagctctt gaattaactt ggctcggtgt aacaggtatt
    13981 ggaggttaca ctccaccaga tactatttca ggtgcgatta ttggtactgc atttggtatt
    14041 ttgtctggtc aaggagaaac tgctggtatc gctatagcag ttccaattgc agttgctact
    14101 caacagttgg atgttcttgc aaaaacttta gatgtttatt ttgtgaaaaa agctgataat
    14161 gatgctaaaa acggagatta ttcaaagatc ggtttttatc attattcaag tttggtttta
    14221 atcactttat ttaaaattgt accaattttc ctagctatta tgcttggagg ggaatatgtg
    14281 gcagacttgt ttgctaaggt tccaccaatc gttatgcagg gacttaactc tgcaggtgct
    14341 ttactacctt caattggttt tggtatgctt ttaaatatga tgctcaagaa aaatatgtgg
    14401 gtattcttgt tgattggatt catttgttct gtgtatggag gaatgtcaac cattgggatc
    14461 tcactagttg gtattgcggt agcatacttc tacgatatga ttggaagtaa accacaagaa
    14521 acaacttcaa gtagtgatgt tgaggaggat cttgatctat gatgaataat aaagtaacta
    14581 aagttgaact taaaaaagtt ttcaaacgaa gttttatgta tggttcttca tggaactatg
    14641 agagaatgca gaacctaggt tttctatata caattcttcc agtattgaaa aaactatacc
    14701 cagacaaaga ttcagcttct cctgcaatga aacgtcacct tgagtttttc aatactcatc
    14761 aaacagcggc accatttatt cttggagtta cttccgctat ggaagaacaa gaaggaaatg
    14821 aaggtgcagc ttcaattact ggtattaaag ttggcttgat ggggccactg gctggtctag
    14881 gagatagttt gttctggctg acactagttc ctatctgttt tagtattggt gcgtcttatt
    14941 ctaaagacgg cggtgcttta ggtatcttta tcgccttaat attgtttaat attattaata
    15001 ttcctgttaa atatttcggt ttgaaatatg ggtatactaa gggttctagt cttatccaag
    15061 aaaataatac aaaaggaaca ttgaatcgcg ttacgagtat ggcgacagca ttagggctag
    15121 tactagtggg tggtttgatt ccatcaatgg ttggtattaa ttttggatta gaatttaagc
    15181 agggggaact tgttatttct gttcaagaaa tgattacaaa attaattcca ggatttatcc
    15241 ctatggcttt gactttatta atgtgtaaat taattagaaa aggaaagaat ccggttgtac
    15301 taatctttag tgttatggct attggagtta tcctagttgt tttaggaatt ttgaagtagt
    15361 agaaagtgtg gaggtggtat ttgggatatc acctccattt tggaagagag gtaaagagtg
    15421 aaattatggt ataagaaagc tgccgcaaat tggaatgaag ccttgccgat tgggaacggt
    15481 catttaggtg gtatgattta tggttcagct acaaaagaat gtattcaact aaacgatgag
    15541 actatttggt atagaggaaa gtcagataga aataatccag actcactatt gcatcttaaa
    15601 aaaattcggg aatatctttt agatggagaa attcagaaag ccgaagaatt gataaagtta
    15661 acaatgtttg ctaccccaag agatcaaagc cactatgaat tacttgggga actttacatt
    15721 gagcatatag atattcagtc ttgtgctctt tcattgtatg aaagagagct agatttagat
    15781 acagctattt ctaatgttgt gtttgagcct aatagttgta atttacaaat aaaaagagaa
    15841 tattttacga gttttaataa gaatatttta tgttgccgta tagtgtcatc agttcaaaac
    15901 acattaaatt taaacattaa tttgggtaga aataaacggt ttaatgatga agtatctaaa
    15961 ctggattcaa gtacaatttt aatgtcggcc tctgctggag gtagaaaagg tgttcagttt
    16021 aaagtagtat gtcattctaa ggttacggat ggtgaagtaa gtgtattggg agagacaata
    16081 gttattcgga atgctacaga ggtatttctt tatctcaaat caatgacgga ttattgggga
    16141 aatatagata tttcttctct tcagggagaa tttagtagta ttgattactt tacagaaaaa
    16201 gatgaacatg taaaaaaata tcaggagcaa tttaatagag ttgattttaa actagactat
    16261 agtaaagatt gtcttagcat tccaacgaat ctacttcttg aaaacactaa aaagtatagt
    16321 aactacttga ctaacttgtt atttcattat ggaagatatc tgttaatatc gtctagtcaa
    16381 ccgaatggtt tacctgccaa tcttcaagga atatggtgtg atgaattaaa tccaatttgg
    16441 ggttctaaat atacgattaa tattaatact caaatgaatt attggatggt aggtccatgt
    16501 gatttaccag aagtagaata tccattattt gatatgctcg aaagaatgag agaaccggga
    16561 agactaaccg ctaagaaaat gtatggagct agaggtttta cagcacatca taatacggat
    16621 ggttttggcg atacggctcc ccaatctcat gccatggggg ctgcaatttg ggtattaact
    16681 attccatggt tatgtactca tatttgggaa cactatttat atttccaaga tgagcgtatt
    16741 cttacggaac attttgaaat gataaaagaa gcatttcttt tctttgaaga ttatttattt
    16801 gaggtggatg gctacttgat gacaggtcca agtgtctcac cggaaaataa atatcgctta
    16861 aaaaatggta ttgaaggaaa tgcttgtcta tcatctacaa ttgataatca aattttaaga
    16921 tatttttgtg attcatgcat tggaattgca aaacaattag gagacaattc ggattttatt
    16981 agtcgtgtga aggagttaaa aaagaaatta cctagaacaa aaataggtag taatgggcaa
    17041 atacaagaat ggctagagga ttatgaagaa gtagagcctg gtcatagaca catttcacct
    17101 ctatttgggc tttatcctta taatgagatt gatattcata aaactccgga attagcagaa
    17161 gcagctaaaa ttactatcaa taggagatta tcaaacgcta actttttatc ttcacaggat
    17221 agggagcaag cgattaataa ttggttagta agtggtttgc atgctagtac acaaacaggt
    17281 tggagtgctg catggctgat tcattttttt gcgagactat atcaaggtga acctgcttat
    17341 aaccagatta atggtttgtt aaataatgcg actcttggca atttatttct tgaccatcca
    17401 ccatttcaaa ttgatggtaa tttaggtttg gtgagtggaa tttgtgaatt attagtacag
    17461 agccatcata attggttatc actaattcca gctttacctt ctgcttggtc agaaggagaa
    17521 gtgaaaggtt tcagagtaag aggaggatat aaggtatcgt ttgcttggaa aaatggggat
    17581 ataacattcc taaaattgga aggaggaaac aaagatcaaa aagtaagagt aagaatatat
    17641 ggcaaaaata c
//
LOCUS       DAVGJL010000029        17520 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.029, whole genome shotgun sequence.
ACCESSION   DAVGJL010000029 DAVGJL010000000
VERSION     DAVGJL010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17520
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.029"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            331..1377
                     /gene="pheS"
                     /locus_tag="VVE42_000850"
     CDS             331..1377
                     /gene="pheS"
                     /locus_tag="VVE42_000850"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEU3073066.1"
                     /translation="MSTIEEQLKALREETLASLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVNGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
     gene            1377..1886
                     /locus_tag="VVE42_000851"
     CDS             1377..1886
                     /locus_tag="VVE42_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU3073067.1"
                     /translation="MIRKVEMADVEVLAKIAKQAFRETFAYDNTEEQLQEYFEEAYSL
                     KTLSTELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GLGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            1963..4368
                     /locus_tag="VVE42_000852"
     CDS             1963..4368
                     /locus_tag="VVE42_000852"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEU3073068.1"
                     /translation="MLVSYKWLKELVDIDVPSQELAEKMSTTGIEVEGVESPAAGLSK
                     IVVGEVLSCEDVPETHLHVCQVNIGEEEARQIVCGAPNVRAGIKVMVALPGARIADNY
                     KIKKGKIRGLESLGMICSLGELGISDSVVPKEFADGIQILPEDAVPGEEVFSYLDLDD
                     EIIELSITPNRADALSMCGVAHEVAAIYDKAVNFKEFTLTETNEAAADALSVSIETDK
                     APYYAARILDNVTIAPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDT
                     FEGTDIRVREARAGEKLVTLDGEERDLDVNDLVITVADKPVALAGVMGGQATEISEKS
                     SRVVLEAAVFNGKTIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASMIAELAGA
                     TVRKGIVSAGELDTSDVEVSSTLDDVNRVLGTELSYADVEDVFRRLGFGLSGNADSFT
                     VSVPRRRWDITIEADLFEEIARIYGYDRLPTSLPKDDGTAGELTVTQKLRRQVRTIAE
                     GAGLTEIITYALTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNV
                     ARKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYA
                     KGILEALFTRLGLAVAYTATAEIASLHPGRTAVISLGDQVLGFLGQVHPVTAKAYDIP
                     ETYVAELNLSAIEAALQPATPFVEITKFPAVSRDVALLLKAEVTHQEVVDAIQAAGVK
                     RLTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEEKVNA
                     EVR"
     gene            complement(4436..5437)
                     /locus_tag="VVE42_000853"
     CDS             complement(4436..5437)
                     /locus_tag="VVE42_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU3073069.1"
                     /translation="MIKTFLSALSVILFVIPIIAYSFFPSSNLNIWLSTRPILAQIYA
                     FPLATATMASILSFVFFSLSFYKKNKQIRFYSGILLLLSLILLLFGTDKTLSSASNKT
                     KTLKLVTWNVANQIEAQHIERIFSHFDADMAIFPELATNIRGEQENQRIKLLFHQVGL
                     SMANYDIFTSPPTNSGIAPVTVIVKKSYGFYTEAKTFHTTRFGTIVLHSRKQNIPDII
                     ALHTAPPLPGLMEIWKQDLNIIHNQLASKYPKAIIAGDFNATMRHGALAKISSHRDAL
                     NVLPPFERGTWNSQSPKLFNATIDHILLPKNHYYVKDLDIVSFQNSDHRCIFTEITF"
     gene            5593..6038
                     /locus_tag="VVE42_000854"
                     /pseudo
     CDS             5593..6038
                     /locus_tag="VVE42_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            6122..6388
                     /locus_tag="VVE42_000855"
     CDS             6122..6388
                     /locus_tag="VVE42_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEU3073070.1"
                     /translation="MEGCPSELFSKEEILESDMRVAIMSELIEARHEQGINQRKLEEL
                     SGVSQPVIARMETGESSLQLDTVLKVLTSLGKTLAVVPLEQGKS"
     gene            6649..8898
                     /gene="metE"
                     /locus_tag="VVE42_000856"
     CDS             6649..8898
                     /gene="metE"
                     /locus_tag="VVE42_000856"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEU3073071.1"
                     /translation="MSTTIIGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKADDFVDSLVAAYQEVFAKLAELGASRIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLLQTYFGDVRDVYADLVNLPVDAIGLDFVEGKKTLELVK
                     GGFPADKTLYVGIVNGKNIWRNNYEKSLAVLEQIPAENIVLTSSCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGEKAELRARIAG
                     LTDADYTRLPVFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVH
                     STVAPDTQIHTHMCYSEFTDIIPAIDNMDADVISFEASRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
     gene            8962..9828
                     /gene="metF"
                     /locus_tag="VVE42_000857"
     CDS             8962..9828
                     /gene="metF"
                     /locus_tag="VVE42_000857"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEU3073072.1"
                     /translation="MSRQTPSLSFEVFPPNPAVGNDNIISALQDMQELAPHFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIEFIKEQTPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
                     DAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQ
                     ATHALFNQQSLG"
     gene            10513..12726
                     /gene="pnp"
                     /locus_tag="VVE42_000858"
     CDS             10513..12726
                     /gene="pnp"
                     /locus_tag="VVE42_000858"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEU3073073.1"
                     /translation="MAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDGQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQE
                     EIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQVVKDQVT
                     AVYEEKYADHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDSEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRLELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHHKDHKPKKEFTETPKDSE"
     gene            12742..13359
                     /gene="cysE"
                     /locus_tag="VVE42_000859"
     CDS             12742..13359
                     /gene="cysE"
                     /locus_tag="VVE42_000859"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEU3073074.1"
                     /translation="MGWWRETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFLW
                     KHGFKLLARMHSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHEVEEKREYYVNKLEQAKDASHRSSGL"
     gene            13371..14255
                     /locus_tag="VVE42_000860"
     CDS             13371..14255
                     /locus_tag="VVE42_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU3073075.1"
                     /translation="MIQVRNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGELVGLLTVYADDQDVEVTILVHPGHRRQGIARALFTSFERETASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYPLANVSHLEILLADSSY
                     QDQISQLKFQAFSEEHESREVVDRYVAEALKDPESRLYILLKAGQVIGTCTVDLSSDT
                     NYFYGLAISEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            14342..15685
                     /locus_tag="VVE42_000861"
     CDS             14342..15685
                     /locus_tag="VVE42_000861"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEU3073076.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRYFEYRGYKVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEDLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLDLGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFATADQIRDQLVTQGIKLLDTKDGVRWTRD"
     gene            15678..16064
                     /locus_tag="VVE42_000862"
     CDS             15678..16064
                     /locus_tag="VVE42_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEU3073077.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLIALMLEEQVLTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEG"
     gene            16068..16952
                     /locus_tag="VVE42_000863"
     CDS             16068..16952
                     /locus_tag="VVE42_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEU3073078.1"
                     /translation="MRAKELQDWFPEARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVALLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLASW
                     LDMMRTLFPNCQTVLQVGAQDYVFVLQQDKYTSVRDILSDTIEAVEYDFGLRLSIMLG
                     QVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
     gene            17195..>17520
                     /locus_tag="VVE42_000864"
     CDS             17195..>17520
                     /locus_tag="VVE42_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073079.1"
                     /translation="MQILCYFTITVVAKPNNSGEVHLDVSIEDNQGGSGHNFSSVSSS
                     SQTAKYEETGYNNNLSLYTTIDKTSDATALLKLKLNNVDNQPATEVPSSGITVKLNTK
                     DNAGNWT"
BASE COUNT         5235 a         3397 c         3981 g         4907 t
ORIGIN      
        1 tcatcttatt tcaatccgct ataaagttag ataagaataa gctaacaagc agaagtaaga
       61 taggtcttgc aagaatggta aaaatctgat aaactaagta gtaattgaat aggaatctga
      121 aagactagta gcccaaggga agtatatcag ggaggagagc cgtgactgca agctctctat
      181 atgaaagctg ggtgaattca cttgcgcatg gatttagaaa tgaaggtctg acttgtcaga
      241 tgaaaacgga tggtaccgcg tgtcaacgct ccgagtggag tttttggcat gtggttttct
      301 ttttatctac gagagactga tggaggaaat atgtcaacta ttgaagaaca attaaaagcg
      361 cttcgcgaag aaacgctggc tagcttgaag cagattactg ctggaaatga aaaagagatg
      421 caagatttgc gtgtctctgt ccttggtaaa aagggttcgc tcactgaaat cctcaaaggg
      481 atgaaagatg tttctgctga gatgcgtcca atcatcggga aacacgtcaa tgaagctcgt
      541 gatgtcttga cagctgcttt tgaagaaaca gctaagctct tggaagaaaa gaaagtcgcg
      601 gctcaactgg ctagcgagag tatcgatgtg acgcttccag gtcgtccagt tgcgactggt
      661 caccgtcacg ttttgacaca aaccagtgaa gaaatcgaag atatcttcat cggtatgggt
      721 tatcaagtcg tgaatggttt tgaagtggag caagactact ataactttga acgtatgaac
      781 cttccaaaag accacccagc tcgtgatatg caggatactt tctatatcac tgaagaaatc
      841 ttgctccgta cccacacgtc tccagttcag gcgcgtgcta tggatgccca tgatttttct
      901 aaaggtcctt tgaagatgat ctcgccaggg cgtgtcttcc gtcgcgatac ggacgatgcg
      961 acccacagtc accaattcca ccaaatcgaa ggcttggtag ttgggaaaaa tatctctatg
     1021 gctgatcttc aaggaacgct tcagttgatt gtccaaaaaa tgtttggtga agagcgtcaa
     1081 attcgtttgc gtccatctta ctttccattc acagagccat ctgttgaggt ggatgtttct
     1141 tgcttcaaat gtggtggaga aggctgtaac gtatgtaaga aaacaggttg gatcgaaatt
     1201 atgggggccg gtatggttca cccacgtgtc cttgaaatga gtggtatcga tgcgactgta
     1261 tactctggct ttgcctttgg tcttggacaa gagcgtgtag ctatgctccg ttatggaatc
     1321 aacgatatcc gtggattcta ccaaggagat gtccgcttct cagaacagtt taaataatga
     1381 ttagaaaagt agaaatggca gatgttgagg tgttggctaa aattgccaaa caagcctttc
     1441 gtgaaacctt tgcgtatgat aatacggaag agcagttaca ggaatacttt gaagaggctt
     1501 atagtctgaa aactttgtca actgagttgg gaaatcctga ctctgaaacc tatttcatta
     1561 tgcatgagga ggagatagct ggttttctca aagtcaactg gggaagtgct caaactgaga
     1621 gagaattaga ggacgctttt gaaattcaac gcctctatgt gctacaaaaa ttccaaggat
     1681 ttggactagg taagcaactg tttgaatttg ctcttgaact tgctacaaaa aatagttttt
     1741 cttgggcttg gttaggtgtt tgggagcata atacaaaagc tcaagccttt tataaccgat
     1801 atggttttga aaaatttagc caacatcatt ttatggttgg tcaaaaagtg gatacggatt
     1861 ggttactgag aaagaaatta aggtaagaag atgattcttc tattgaaatg ataggaaagg
     1921 aaaagaacta gagtgacaac tgtcattctg gagaactaaa ttatgcttgt atcttataaa
     1981 tggttaaaag aattggtgga cattgatgtg ccatcacagg agttggctga aaaaatgtca
     2041 accacaggaa tcgaggtaga gggtgtcgaa tcaccagctg ccggtctttc aaaaattgtc
     2101 gtcggtgagg tcttgtcttg cgaagatgtg ccagaaactc acctccatgt ttgtcaggtt
     2161 aacattggcg aagaagaagc tcgtcaaatc gtttgtggtg ccccaaatgt gcgtgcaggc
     2221 atcaaggtta tggtggctct tccgggagct cgtatcgctg acaattacaa aatcaaaaaa
     2281 ggaaaaatcc gtggcttaga gtcacttgga atgatctgtt cacttggtga attgggtatt
     2341 tctgactcag ttgtacctaa ggaattcgca gatggcatcc aaatcttgcc tgaagatgcc
     2401 gtgccaggtg aggaagtctt ctcatatcta gacttggatg atgaaatcat cgaactttcc
     2461 atcacaccaa accgtgcaga tgccctttct atgtgtggag tggctcacga agtggcagcc
     2521 atctatgaca aggcagtcaa ctttaaagaa tttactctaa cagaaactaa tgaagctgcg
     2581 gcagatgccc tttctgtcag cattgagaca gacaaggcgc cttactatgc agctcgtatt
     2641 ttggacaatg tgaccatcgc accaagtcca caatggttgc aaaaccttct catgaacgaa
     2701 ggaatccgtc ccatcaataa cgtagtggac gtgacaaact acatcctgct ctattttggt
     2761 caaccaatgc atgcctttga cttggacaca tttgaaggga ctgacatccg tgtgcgtgaa
     2821 gcgcgtgctg gtgaaaaatt ggtgaccttg gacggtgaag aacgtgactt ggacgtgaat
     2881 gacctagtca tcactgttgc agacaagcca gtagcccttg caggtgtcat gggtggtcaa
     2941 gcaacagaaa tctctgaaaa atctagtcgt gttgtccttg aagctgctgt tttcaatggc
     3001 aaaactatcc gtaagacaag tggtcgcctg aaccttcgtt ctgagtcatc ttctcgcttt
     3061 gaaaaaggaa ttaatgtggc aacagttaat gaagcccttg atgcggcagc tagcatgatt
     3121 gcagagcttg caggcgcgac ggtgcgtaag ggtatcgttt cagcgggtga gcttgatacc
     3181 tctgatgtgg aagtttcttc aacccttgat gatgttaacc gtgtcctcgg aactgagctg
     3241 tcttatgctg atgtagaaga cgtcttccgt cgtcttggct ttggtctttc tggaaatgca
     3301 gacagcttta cagtcagcgt accacgtcgt cgttgggata tcacaatcga agctgatctc
     3361 tttgaagaaa tcgctcgtat ctatggatat gaccgcttgc caaccagcct tccaaaagac
     3421 gatggtacag ctggtgaatt gactgtgaca caaaaactcc gccgtcaagt tcgtaccatt
     3481 gctgaaggag caggtttgac agaaattatc acctatgctc tgacaactcc tgaaaaagca
     3541 gttgagttca cagctcaacc aagtaacctt actgaactca tgtggccaat gactgtggat
     3601 cgttcagtcc tccgtcaaaa tatgatttca gggatccttg ataccgttgc ctacaacgtg
     3661 gctcgtaaga ataaaaactt ggccctttat gagattggaa aagtctttga acaaacaggt
     3721 aatccaaaag aagaacttcc aaatgagatc aacagctttg cctttgcctt gacaggcttg
     3781 gttgctgaaa aagatttcca aacagcagca gttccagttg atttcttcta tgctaaagga
     3841 atcctagaag cgctctttac tcgcttggga cttgcagtgg cttatacagc aacagctgaa
     3901 attgctagcc ttcatccagg tcgaacagcc gtgatttcac tcggtgacca agttcttggt
     3961 ttccttggcc aagtgcatcc agtcactgct aaggcttatg atattccaga aacgtatgta
     4021 gctgagctta acctttcagc tatcgaagct gcgcttcagc cagcgactcc atttgtagaa
     4081 atcaccaaat tcccggcagt cagccgtgac gttgcccttc tcctcaaggc agaagtgact
     4141 catcaagaag ttgtagatgc tatccaagct gccggcgtga aacgtttgac agatatcaaa
     4201 ctctttgacg tcttctcagg tgagaaattg ggacttggta tgaagtcaat ggcttatagc
     4261 ttgaccttcc aaaatccaga agatagctta acggacgaag aagtcgcacg ctatatggaa
     4321 aaaatccaag catcgctcga agaaaaagtg aatgcagaag tgcgttaaac gatagtatga
     4381 tacaaattcc ccctctagtt tatgaacact agaggggtga ttttatataa aataattaaa
     4441 atgtgatttc tgtaaaaata catctatgat cagagttttg aaaacttaca atgtctaaat
     4501 ctttaacata gtagtggttt ttaggcaata aaatatgatc tattgttgca ttaaaaagtt
     4561 ttggactttg gctattccaa gttcctcttt caaaaggtgg cagtacattt aatgcgtccc
     4621 tatgagagct tatttttgca agtgctccat gacgcatagt tgcattaaaa tcacctgcaa
     4681 taatagcctt tggatatttt gaagccaatt gattatgaat gatgtttaag tcttgcttcc
     4741 agatttccat taaacctggc agaggaggag cagtatgcaa ggcaatgata tctggtatat
     4801 tttgttttct cgaatgtaat acaattgtcc caaaccgtgt tgtatgaaaa gttttagctt
     4861 ctgtatagaa accataactt ttcttgacaa tcacagtcac aggagctatt ccactattgg
     4921 taggtggaga agtgaaaata tcatagttgg ccatagaaag tccaacttga tgaaacaata
     4981 gtttgattct ctggttttct tgctcacctc tgatattggt agctagttca gggaatatag
     5041 ccatatcggc atcaaaatgg ctaaaaattc gctcaatatg ttgtgcttct atttgattag
     5101 cgacgttcca agttactaat tttaaggttt tagtcttatt tgatgcagaa gaaagggttt
     5161 tatctgttcc gaataatagt aatatgagcg atagtaagag caaaatgcca gagtaaaacc
     5221 gtatttgttt atttttcttg taaaaagata gggaaaaaaa tacgaaactt aaaatagaag
     5281 ccatagttgc agtagctaag gggaaggcat aaatctgtgc caagataggt ctggtagata
     5341 gccaaatatt aagattagaa gatggaaaaa aagaataagc tatgataggg ataacaaaga
     5401 gaatgaccga aagggcagag agaaatgttt taatcattgt gtgagtacct caatttgcaa
     5461 tggttgctcg gagctaatgt gactttgtga ctttacttga ttgatattct ttttattgtc
     5521 ttccttatag attaagtaca acccaagtaa aaaaagtcca atttaagatt gaaatgatta
     5581 aaaggcacag aaatgcaaga caattatact acaaaaggca aacatttgac aatcgatagc
     5641 cgtcgcttaa tcgaacgatg gaaaaaagaa ggaaaatcaa atagagaaat tgcctctcta
     5701 cttggaaaag ctcctcaaac tatccatact gaaatcaagt atgggacagt ccgacaatgt
     5761 cttggaaaag ggcgcttcaa agagatttat tctgccgact acgctcaaca gtcttaagaa
     5821 actaaaggaa aagattctcc actatacata accaaaaatt ttcgcctgaa atgatggtta
     5881 tggctaaagg ggttaacgtg ggaatttcaa ccatttacta ttggattcat catggaaaat
     5941 tggggttaag caaacaggat ttgctttatc ctagaaaagg aaaagcgctt aagaaacaag
     6001 ctagtcccaa ttttaaacct gctggtcagt ctatcgaatc ataattcgct cattttttca
     6061 aaaatacata gacgtaaaag aaagagggtt aagtgatgaa gaatagtgct attgggagta
     6121 attggaagga tgtccatctg agctctttag caaggaggaa atccttgaaa gtgatatgcg
     6181 agtggctatt atgagcgagt tgattgaagc tagacatgaa caaggaatca atcagagaaa
     6241 gctagaagaa ctcagtggag tgagtcaacc tgtcatagct aggatggaaa caggagagag
     6301 cagtcttcag ttggatacgg tcttaaaagt tctaaccagt ctaggaaaga cactagcagt
     6361 cgttccactt gaacagggga aaagttgact agaatcagtt atagtctttt ctaataacag
     6421 gtcatagtca cttataagaa ttactaataa cgcgtttgaa ccgtctaact gaaataaagc
     6481 tataaaacaa gctatacaaa ggatttgaga cgtttgcttc aagtcttttt cttttgtccg
     6541 aaacagatat atagtttcaa actatatcaa accctaattt tttacaatta tacagacgct
     6601 ttcttttctg ttaagatagt ttcaacaaca aattttggag gacacatcat gtcaactaca
     6661 atcatcggtt tccctcgttt gggcgaattc cgtgaattaa aatttacaac tgaaaaatac
     6721 tttagaaaag aaatctcaga agaagaactt ttagcagcag caaaagactt gcgtgctaaa
     6781 cactggaaca ttgtcaaaga aaaaggcatc actgaaattc catcaaatga cttttctcac
     6841 tatgataact tcctagatgc ggctttcctt ttcaacgtgg tgcctgcatc cgttcaaaac
     6901 ttggacttgt ctgaccttga gcgctacttc gctttgggac gtggttacca gggagaaaaa
     6961 ggggacgttc gcgcccttcc aatgaagaaa tggttcaaca ctaactacca ctacatcgtt
     7021 cctaaatttg aaaaagacac tcaagtaaaa ttggctggtc acaagatttt cgatgagttc
     7081 caagaagcca aagaacttgg attgaacact cgtcctgttc ttgtaggtcc cttcactttc
     7141 cttcaattgt cagactttga agaaggcgtg aaagcagacg acttcgtaga tagcttagtg
     7201 gctgcttacc aagaagtttt tgctaaattg gctgaacttg gtgcgagtcg catccaattg
     7261 gatgaagcgg ctcttgtaaa agacttgaca gctgaagaaa aagctctctt cttgaacctt
     7321 tacaacaaac tcttggctga caaaaaaggt cttgaagtct tgcttcaaac ttatttcggt
     7381 gatgttcgtg acgtatacgc tgaccttgta aacttaccag tagatgctat cggtcttgac
     7441 tttgttgaag gtaagaaaac tcttgaactc gttaaaggtg gcttcccagc tgacaagact
     7501 ctctatgtag gtattgtcaa tggtaaaaac atctggcgca acaactacga aaagagcttg
     7561 gctgttcttg agcaaatccc agctgaaaac atcgttttga caagctcttg ctcacttctt
     7621 catgtgccat ttacaactgc taatgaagaa tttgaaccag cactcttgaa ccactttgcc
     7681 tttgcagttg aaaaattgga tgaaatccgt gacttggatg ctatccgcaa tggtcaaggt
     7741 tcagaagctc ttgcagccaa caaagaactc tttgcgactg agcgtgttgg tgaaaaagct
     7801 gaacttcgtg cgcgtatcgc tggcttgaca gacgcagact acactcgttt gccagtcttt
     7861 gcagaacgtg aagctatcca agaagaagct ttcaaacttc cagctcttcc aacaacaacg
     7921 attggttcat tccctcaaac aaaagaagtt cgtgccaaac gtttggctta ccgtaaaggt
     7981 gaattgtctc aaaaagagta cgatgctttc cttgctgaaa cgattgacga atggatcaag
     8041 tggcaagaag atattgactt tgatgtcctt gttcacggtg aatttgagcg taatgacatg
     8101 gttgagtact tcggtcaaaa cttgtcagga tacctcttct ctaaaaatgg atgggtacaa
     8161 tcatacggta tgcgtggggt aaaaccacca atcatctggg gtgatgtcac tcgtcttaac
     8221 cctatcactg ttaaatggtc tagctatgca caaagtcgta caaacaaacc tgttaaaggt
     8281 atgttgactg gacctgttac catccttaac tggtcattcc cacgtgaaga catctctatc
     8341 aaggattcaa ctctccaaat cgcccttgct atcaaggatg aagtacttga ccttgaagct
     8401 gctggtgtga aaatcatcca aatcgacgag gctgctcttc gtgaaaaatt gccactccgc
     8461 cgtagcgact ggtacgaaga ctaccttgac tgggctattc ctgccttccg cttggtacac
     8521 tcaacagtag cgccagacac acaaatccac actcacatgt gttactcaga atttacagat
     8581 atcatcccag ctatcgataa catggatgca gacgttattt cctttgaagc tagccgttca
     8641 aaccttgaaa tcttggacga actcaaagcg aaaaacttcc aaacagaagt gggacctggg
     8701 gtttacgata tccactcacc tcgtgtacca aatgaaggcg aaatcgacaa cacaatcgaa
     8761 gccatccttg ctaaagtgcc aagcaagaaa gtttggatca accctgactg tggtttgaaa
     8821 acacgtggta tcccagaaac aaaagaaagc ttgatccgcc ttgttgaagc agctaaagct
     8881 gcgcgtgaga aattgtaaga ttggatttct ctccatacag tggttgttca aaaagaaatc
     8941 aactagaaaa ggttattaca tatgtcacgc caaacaccgt cactctcatt tgaagtgttc
     9001 cctccaaacc cagccgtggg taatgataac attatttcag ccttgcagga tatgcaggag
     9061 ttggctcccc attttatcag tgtaactgcc agcaataata aatttaatat caaggaaacg
     9121 acggttcgtt tggctgactt tatccaaaat gatttggcga ttccgaccat tgcccacttg
     9181 ccagctattt atctaactaa ggacaaggtt gctgaaacta ttgctgactt ggacaaagtt
     9241 ggggtgcaga aaatcttggc tcttcgtggg gatattattc cagacgtgga accacaaaag
     9301 gatttccgct atgcaaccga cttgattgag tttatcaagg aacaaacccc tcactttgat
     9361 attattggag cttgctatcc agaagggcat ccagattcgc caaatcagat ttcagatatt
     9421 caaaatctta agaagaaagt ggatgcaggc tgttcgagcc tcgtaactca gctcttcttt
     9481 gacaatgagc gcttctatga ttttcaggac aagtgcatct tggctgggat tgatgttccc
     9541 attcatgcag gaattatgcc aattctgaat cgaaatcagg ctctccgact cttgaagact
     9601 tgtgagaata tccatcttcc acgcaaattt aaagccatct tagacaagta tgagcatgac
     9661 cctgagtcgc tcagagcagc aggacttgcc tatgcagtgg accaaatcgt ggacttggta
     9721 actcaggatg ttgccggtgt gcatctctat acgatgaata atgctgatac agcaaaatac
     9781 atccatcaag caacccatgc cttgtttaat caacagtctc taggataata aaagcaaacc
     9841 attcttctca ggtgagggga atggttcctt tttaatggta aagacctcac tttttaagaa
     9901 aaatatgata aaataaattc tgtacgtact tgatacaaag atgaaggtat taaataatac
     9961 aatgcattta aactgtaaga tttaacgaca cgagtgagtc aaatcgatgt tatttgacga
    10021 gagagttagt aaagcattta gctaatcgcc cgatagcgat tagcgtgaaa gctttagata
    10081 ctttagtatt aaacctttct aatgatgtat cttgaaaact gaacttgcct acaactctgt
    10141 ggaaaaagat aaatctccct aggagcagtc gctccgtcgt tcgatttcct atcttccttt
    10201 gagttgcttg acggcttggt atcttgatga cttgaatttt ggtctcattt cttaggaaaa
    10261 aagataagct tcctagcact cgtcgagtgc gacgtcacct tcctattttt ctacagaaat
    10321 gttcggcaag ccgaaccgtc caaaatatct tgtgtaattg aacacggccg aaaagctgtg
    10381 taaaaaagat aaactgtctt gtcttcatcg aagacttcgt cagtttccta tttttacttt
    10441 gcttttgacg tccttggtat cttgatcttt gtaggcaagg cgtataattt catcaatcca
    10501 aaggggatta aaatggcaaa acaagtgttt caaacgactt ttgcgggtcg tgagttaatt
    10561 gtagagactg gtcaggttgc taagcaagca aatggatctg ttgtcgtacg ttacggtgag
    10621 tcaactgtct tgactgctgc cgttatgtct aagaaaatgg caactgggga tttcttccca
    10681 ctccaagtca actacgaaga aaaaatgtat gcggctggga agtttcctgg tggctttatg
    10741 aaacgtgaag gacgtccttc aacagatgcg accttgacag cgcgtttgat tgaccgtccg
    10801 attcgtccta tgtttgcgga aggtttccgt aatgaagtcc aagtcatcaa tacagtgctt
    10861 tcttatgatg aaaatgcatc tgcaccaatg gctgctatgt ttggttcatc tttggcactg
    10921 tctatttcag atattccatt tgacggacca attgctgggg tacaagtggg atatgtagat
    10981 ggccaaatca tcatcaaccc aagtcaagaa caagcagagc aatctcttct tgaattgaca
    11041 gtagctggaa ccaagcacgc tatcaacatg gtagagtctg gtgccaaaga attgtcagaa
    11101 gaaatcatgt tggaagcgct tcttaaaggg cacgaagctg tcaaagaatt gattgccttc
    11161 caagaagaaa tcgttgctgc tgtcggtaaa gaaaaagcag aagtggaatt gcttcacgtg
    11221 gatgctgaat tgcaagctga aatcattgca gcctacaaca gtgacctcca aaaggcagtt
    11281 caagtagaag agaaattggc ccgtgaagct gcgactcaag tagtgaaaga ccaagtgact
    11341 gccgtttacg aagaaaaata tgcggaccat gaagaatttg accgtattat gcgtgatgtg
    11401 gctgaaatct tggaacaaat ggaacacgca gaagtgcgac gtttaattac agaagacaag
    11461 gtgcgtcctg atggtcgtaa ggtcgatgaa atccgtcctt tggatgcggt tgttgacttc
    11521 cttcctcgtg tacatggttc aggtctcttt actcgtgggc aaactcaagc tctttcagtc
    11581 ttgaccttgg ctccgatggg agaaactcaa atcattgatg gtttggattc agagtacaag
    11641 aaacgcttta tgcaccacta taacttccct caatattctg taggggaaac aggtcgttac
    11701 ggtgcgccag gtcgtcgtga aatcggtcac ggtgcccttg gtgagcgtgc tcttgctcaa
    11761 gtcttgccaa gcttggaaga attcccatac gctatccgtc tagtagcaga agttttggaa
    11821 tcaaacggtt cttcatctca agcttctatc tgtgcgggaa ctcttgccct tatggctggt
    11881 ggtgtgccaa tcaaggcgcc agtagctggt attgctatgg gacttatctc agatggaaat
    11941 aactacacag tattgacaga tatccaaggt ttggaagatc actttggaga tatggacttc
    12001 aaggttgcag gtactcgtga tgggattaca gcccttcaaa tggatatcaa gattcaaggg
    12061 attactgcag aaatcttgac ggaggctctt gctcaagcca agaaagcgcg ttttgaaatc
    12121 cttgatgtca ttgaagcaac cattccagaa gttcgtctag aattggctcc aactgctccg
    12181 aaaattgata cgatcaagat tgatgtggac aagattaaga ttgtcatcgg taagggtgga
    12241 gaaaccatcg acaagattat cgctgaaaca ggtgttaaga ttgatataga cgaagaagga
    12301 aatgtgtcta tctactctag tgaccaagat gctattaacc gtgccaaaga aattattgct
    12361 ggtttggttc gtgaagccaa agtggatgaa gtttaccgtg ctaaagtcgt tcgtatcgag
    12421 aaatttggtg cctttgttaa cctctttgat aagacagatg cccttgttca tatctctgag
    12481 atggcttgga ctcgtaccaa tcgtgtagag gatttggtag aaatcgggga tgaagttgat
    12541 gttaaggtta tcaagattga tgaaaaaggc cgtatcgatg cctctatgaa ggctcttcta
    12601 cctcgtccgc caaaacctga gcatgatgaa aaaggtgaaa agtctgagcg tcctcaccgc
    12661 ccacgtcatc acaaggatca caaacctaag aaagaattta cagaaacacc aaaagattca
    12721 gaataagaaa aggagaaatg tatggggtgg tggcgcgaaa ccattgatat cgtaaaagaa
    12781 aatgatccag cggcccgcac cactttggag gttttgctga cttatccagg tgtcaaggcc
    12841 ttggcggccc accgtctctc gcattttctc tggaagcacg gcttcaaact cttagctcgt
    12901 atgcacagtc agttttggcg cttttggact cagattgaga ttcatccagg agcccagatt
    12961 gattcaggtg tctttattga ccatggttct ggtctggtga ttggagagac agcgattgtt
    13021 gaaaaaggcg ttcttctcta tcacggagtg actcttgggg gaacagggaa agactgtggc
    13081 aaacgccatc cgactgtacg aaagggagcc ctcatatcag cccatgccca agttatcgga
    13141 cctgtggaaa ttggtgaaaa tgccaaggtc ggtgctgcag cagttgtcgt agcagatgta
    13201 cctagtgatg tgacggttgt cggtattccg gccaagattg tccgtcttca tggtaagaaa
    13261 gatgagcctg tcattcacga agtcgaagaa aaacgagagt attatgtcaa taaactcgag
    13321 caggctaaag atgccagtca cagatcgtct ggtttgtaga ggatacctat atgattcaag
    13381 taagaaacaa gttaagccaa gaggagctat ctgaggcgaa aaaagtaatt aactgttgcc
    13441 aaaactatga cggtacctat cgcgatccct atctctctaa catgcttaat tttgacccaa
    13501 acatgcccgc ctttttcctt tattatgaaa aaggcgaact tgttggttta ttaactgtct
    13561 atgcagatga ccaagatgta gaagtgacga tactggttca tccaggtcat cgccgtcagg
    13621 ggattgcgcg tgcattgttt actagttttg agagagaaac agcttctttc cccattcggt
    13681 cagtcacttt tcagacagaa cgtatttttt tagagaatca tcctgacttt gccagtaact
    13741 ggggactaat cgaggatgaa gagacagaaa cttggttagg taaggataga agaccttatc
    13801 cgttagcaaa tgtttcccat cttgaaattt tgttagcaga tagttcgtat caggatcaaa
    13861 ttagtcaatt aaaatttcag gcattttcag aggaacatga atcgagagaa gttgtggata
    13921 gatatgtcgc tgaagctctg aaagatccag aaagccgact atatatttta ttaaaagctg
    13981 gtcaggttat tgggacttgc acggttgatt tatcgagcga tacgaattac ttctacggtt
    14041 tagcaatatc agaacctgaa cgtgggaaag gctatggaag ctacttagca aaatcccttg
    14101 tcaaccaact aattgagcaa aatgacaagg aatttcagat tgcagtggaa gatagcaatg
    14161 taggtgccaa acgtttgtat gaaaaaattg gctttgtcaa acagacacag gtggtttatc
    14221 tgaatgagaa aggagcaagg gattccgaag tgtagagaca ttcggactga aatttacttg
    14281 agctcttagt ttagtgatga aactaattgt tcttggattt cagctttcct gattatgatt
    14341 tatgattaaa atctatgaca ccatgtctcg tgatttgcga gaatttgtcc cgattgagga
    14401 cggcaagatc aagatgtatg tttgtgggcc aaccgtgtac aactatatcc acgtgggaaa
    14461 cgcccgttcg acggtagctt ttgatacgat tcgtcgctat tttgagtacc gtgggtataa
    14521 ggttgcctat atttccaatt ttacagatgt ggatgataag attatcaacc gtgccaggga
    14581 agaaggtatc acgcctcagg aggttgcgga taagtacatc gctgcctttc gtgaggatgt
    14641 gacggcctta ggcgtgaaac ctgcgactcg ccatccgcgt gtagtggagt ttatggcaga
    14701 catcatccgt tttgtggaag acttgatcga gaaaggcttt gcctatgaga gtcaagggga
    14761 tgtctatttc cgtgtagaaa aatcccacaa ctatgctaaa ttggctaata aaaccttgga
    14821 agatttggat ctaggtgctt caggtcgtac cgatgaagaa acggctcgta aggaaaatcc
    14881 tgtagatttt gctctatgga aatcttccaa accaggtgag atttcttggg atagtccttg
    14941 gggacctgga cgtccgggtt ggcatattga gtgttcagtc atgtcgacag agattttagg
    15001 tgataccatt gatatccacg gtggtggagc tgacctagag tttccacacc acaccaatga
    15061 aattgcccaa tctgaagcta aaacaggcaa ggcttttgct aactactgga tgcacaatgg
    15121 ctttgtcaat atcgacaatg tcaaaatgtc taagtccttg ggtaacttca ttacggtaca
    15181 cgatgccctt aaaactcttg atgggcaagt acttcgtttc ttctttgcga cccagcacta
    15241 ccgtaagcct atcaacttca cggaaaaggc agttcgcgat gccgagacca atctcaagta
    15301 tctgaagaat acttacgaac aaccatttac aggaaacgtg gatgcccaag agttacaaaa
    15361 ctttaaagat aagtttgtag cagctatgga tgaagatttt aatgctgcca acggtatcac
    15421 agttgtcttt gaaatggcca aatggatcaa ttcagggaac tatgatgcaa gtgtcaagca
    15481 agctcttgca gatatgttag aaatttttgg aattgtcttt gttgaggaag ttttggatgc
    15541 agagattgaa gacttgattc aaaaacgcca agaggcgcgt gccaatcgtg actttgcgac
    15601 agcagaccaa atccgtgacc aattggttac tcaaggaatt aagctccttg ataccaagga
    15661 tggagtgagg tggacacgtg attgatgtca atctcattaa cgggattgcg ctagcctttg
    15721 agggggatgc ggtgtattct atgtatattc gccgtcacct catcctcaaa ggtatgacca
    15781 aacccaataa actccatcaa gaagcaacca agtatgtgtc agccaaggct caggctcgcc
    15841 tgattgctct catgttggag gagcaggtcc taacggaaaa agaagaagaa atctacaaac
    15901 gtggccgcaa taccaatagc cacacaaagg ctaaaaatgc agatgtcgtg acttatcgta
    15961 tgtccacggg atttgaagcg gttatgggct atctccatat gactgagaat ctggaacgtc
    16021 ttgagagttt ggtttcatgg tgcatccaaa aagtggaggg ctagaacatg agggcaaaag
    16081 aactacaaga ctggtttcct gaggctcgga tttcagacca accagtagag aaagagggct
    16141 atctcacgct ccctttagct tctcagcagt ggattttgct ggaggaagct gggctcagcg
    16201 agcgtgaaaa gcagttggtt gcccttttga cccagcagga gcaggctcgt tcgctaaacc
    16261 cttggtattc ctatctggtt gaaggcaagg gacaggcacc gcaagttttt aaaaagattc
    16321 agttggttta ttgccatctt tcttattttc agcaggaaaa tctggcttct tggctagata
    16381 tgatgcggac tctttttccg aattgtcaga cagtgctaca ggtcggagct caggattatg
    16441 ttttcgtgct tcaacaagac aaatacactt ctgtaagaga tattttaagt gatacgattg
    16501 aagcggttga gtatgacttt ggacttcgtc tttctatcat gttgggtcag gtttggtctc
    16561 agacaggaca tcaagcccta tcagacttaa tcaaagctga gcgggatttg ttcaagacat
    16621 ggtggcgtca gggtcaccaa ggtgttcata ctttttctca gctctatctt tggagtatgg
    16681 gagaaagact cgtggacttg aagccaatta aagaatgtct acaccagatg attttggatc
    16741 aagatcagat tcaggaaatc attctctctc tttgggaaaa tagtgctgtt ctcactaaaa
    16801 cagcccagca actctatctg caccgcaatt ctctccaata caagattgat aaatgggaag
    16861 agttgacagg gcttcagttg aaagagttga ccgacctgac cttgtgttat caattgattt
    16921 taggttcttt gtcaactata gttggtttgt aaagaagtta atatttggag aagaggatgg
    16981 ccatcttctc catttttatg tgcagagtta tagtgacttg atgctgggaa agtacactgt
    17041 gactgctaat actcaatgaa aatcaaagtg caaactagga agctagccgc aggctgtact
    17101 tgagtacgtc aaggtgaagc tgacgtggtt tgaatttgat tttcgaagag tataaaacat
    17161 tcctagaagt tgctttgatt tccctaatct atttatgcaa attttatgtt attttactat
    17221 aacagttgtt gctaagccaa ataatagtgg ggaagttcat ttagacgtaa gcattgaaga
    17281 taatcaggga ggtagtgggc ataatttcag ttctgtttca agtagctcac aaacagctaa
    17341 atatgaagaa actggttata ataacaattt atcattatat acaacgattg ataaaacgtc
    17401 tgatgcaaca gctcttttga aattaaagtt gaataatgtt gataatcaac ctgctactga
    17461 agttcctagt tcaggaatta ctgtaaaatt aaatactaaa gataatgctg gaaactggac
//
LOCUS       DAVGJL010000030        17258 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.030, whole genome shotgun sequence.
ACCESSION   DAVGJL010000030 DAVGJL010000000
VERSION     DAVGJL010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17258
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.030"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            29..5269
                     /locus_tag="VVE42_000865"
     CDS             29..5269
                     /locus_tag="VVE42_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEU3073080.1"
                     /translation="MIGAAFFGTSPVLADSVQSGSTANLPADLATALATAKENDGRDF
                     EAPKVGEDQGSPEVTDGPKTEEELLALEKEKPAEEKPKEDKPAAAKPETPKTVTPEWQ
                     TVEKKEQKGTVTIREEKGVRYNQLSSTAQNDNAGKPALFEKKGLTVDANGNATVDLTF
                     KEDSEKGKSRFGVFLKFKDTKNNVFVGYDKDGWFWEYKTPGNSTWYKGNRVAAPEPGS
                     VNRLSITLKSDGQLNASNNDVNLFDTVTLPAAVNDHLKNEKKILLKAGTYGNDRTVVS
                     VKTDNQEGVKADDTPAQKETGPVVDDSKVTYDTIQSKVLKAVIDQAFPRVKEYTLNGH
                     TLPGQVQQFNQVFINNHRITPEVTYKKINETTAEYLMKLRDDAHLINAEMTVRLQVVD
                     NQLHFDVTKIVNHNQVTPGQKIDDERKLLSSISFLGNALVSVSSDQTGAKFDGATMSN
                     NTHVSGDDHIDVTNPMKDLAKGYMYGFVSTDKLAAGVWSNSQNSYGGGSNDWTRLTAY
                     KETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKELPSAKVVITEDANADKKVDWQDGA
                     IAYRSIMNNPQGWEKVKDITAYRIAMNFGSQAQNPFLMTLDGIKKINLHTDGLGQGVL
                     LKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIEKAKKYGAHLGIHVNASETYPESKY
                     FNEKILRKNPDGSYSYGWNWLDQGINIDAAYDLAHGRLARWEDLKKKLGDGLDFIYVD
                     VWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWGHGGEYDSTFHHWAADLTYGGYTNK
                     GINSAITRFIRNHQKDAWVGDYRSYGGAANYPLLGGYSMKDFEGWQGRSDYNGYVTNL
                     FAHDVMTKYFQHFTVSKWENGTPVTMTDNGSTYKWTPEMRVELVDADNNKVVVTRKSN
                     DVNSPQYRERTVTLNGRVIQDGSAYLTPWNWDANGKKLPTDKEKMYYFNTQTGATTWT
                     LPSDWAKSKVYLYKLTDQGKTEEQELTVKDGKITLDLLANQPYVLYRSKQTNPEMSWS
                     EGMHIYDQGFNSGTLKHWTISGDASKAEIVKSQGANDMLRIQGNKEKVSLTQKLTGLK
                     PNTRYAVYVGVDNRSNAKASITVNTGEKEVTTYTNKSLALNYVKAYAHNTRRDNATVN
                     DTSYFQNMYAFFTTGSDVSNVTLTLSREAGDQATYFDEIRTFENNSSMYGDKHDTGKG
                     TFKQDFENVAQGIFPFVVGGVEGVEDNRTHLSEKHDPYTQRGWNGKKVDDVIEGNWSL
                     KTNGLVSRRNLVYQTIPQNFRFEAGKTYRVTFEYEAGSDNTYAFVVGKGEFQSGRRGT
                     QASNLEMHELPNTWTDSKKAKKVTFLVTGAETGDTWVGIYSTGNASNTRGDSGGNANF
                     RGYNDFMMDNLQIEEITLTGKMLTENALKNYLPTVAMTNYTKESMDALKEAVFNLSQA
                     DDDISVEEARAEIAKIEALKNALVQKKTALVADDFESLDAPAQPGEGLENAFDGNVSS
                     LWHTSWNGGDVGKPATMVLKEPTEITGLRYVPRGSGSNGNLRDVKLVVTDESGKEHTF
                     TATDWPDNNKPKDIDFGKTIKVKKIVLTGTKTYGDGGDKYQSAAELIFTRPQVAETPL
                     DLSGYEAALAKAQKLTDKDNQEEVASVQASMKYATDNHLLTERMVEYFADYLNQLKDS
                     ATKPDAPIVEKPEFKLRSLASVQGKTPDYKQEIDRPDRPETPEQILPATGESQSDTAL
                     FLAGVSLALSALFVVKTKKD"
     gene            complement(5436..7595)
                     /locus_tag="VVE42_000866"
     CDS             complement(5436..7595)
                     /locus_tag="VVE42_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEU3073081.1"
                     /translation="MNKPTILRLIKYLSISFLSLVIAAIVLGGGVFFYYVSKAPSLSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTDLVKAIVSIEDHRFFDHRGID
                     TIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAVNTPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEEETGYNLLTTGMDVYTNVDQEAQKHLWDIYNTDEYVAYPDDELQVASTIV
                     DVSNGKVIAQLGARHQSSNVSFGINQAVETNRDWGSTMKPITDYAPALEYGVYDSTAT
                     IVHDEPYNYPGTNTPVYNWDRGYFGNITLQYALQQSRNVPAVETLNKVGLNRAKTFLN
                     GLGIDYPSIHYSNAISSNTTESDKKYGASSEKMAAAYAAFANGGTYYKPMYIHKVVFS
                     DGSEKEFSNVGTRAMKETTAYMMTDMMKTVLSYGTGRNAYLAWLPQAGKTGTSNYTDE
                     EIENHIKTSQFVAPDELFAGYTRKYSMAVWTGYSNRLTPLVGNGLTVAAKVYRSMMTY
                     LSEGSNPEDWNIPEGLYRNGEFVFKNGARSTWNSPAPQQPPSTESSSSSSDSSTSQSS
                     STTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP"
     gene            complement(7592..8188)
                     /gene="recU"
                     /locus_tag="VVE42_000867"
     CDS             complement(7592..8188)
                     /gene="recU"
                     /locus_tag="VVE42_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEU3073082.1"
                     /translation="MVNYPHKVSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLAVIHKKPTPIQIVQVDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEVKETKQ
                     KRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKGQK
                     SMPLEYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            8254..8781
                     /locus_tag="VVE42_000868"
     CDS             8254..8781
                     /locus_tag="VVE42_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEU3073083.1"
                     /translation="MATALVLGYSAFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGSLGFEYWVLEVAQEMKTEYGFQLATIFAFETHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTTSSYLFYDEENETKLAYFYQKMKNQEDYFIKRL
                     TFDQLNELAENFSEN"
     gene            8851..9180
                     /gene="gpsB"
                     /locus_tag="VVE42_000869"
     CDS             8851..9180
                     /gene="gpsB"
                     /locus_tag="VVE42_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEU3073084.1"
                     /translation="MASIIFSAKDIFEQEFGREVRGYNKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELTRKPKPSPVQAEPLEAAITSSMTNFDILKRLNRLEKEVFGKQ
                     ILDNSDF"
     gene            9201..9581
                     /gene="rnpB"
                     /locus_tag="VVE42_000870"
     ncRNA           9201..9581
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VVE42_000870"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            9666..10823
                     /locus_tag="VVE42_000871"
     CDS             9666..10823
                     /locus_tag="VVE42_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEU3073085.1"
                     /translation="MKKEFNLIATVAAGLEAVVGREVRELGYDCQVENGRVRFQGDVR
                     AIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARFPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFEEWNWISDRLIQEVRTEAAKKVDRELELDIMGCDIDARMVEIAKANA
                     QAAGVAGDITFKQMRVQDLRSDKINGVIISNPPYGERLSDDAGVTKLYAEMGQVFAPL
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            10836..12230
                     /gene="mapZ"
                     /locus_tag="VVE42_000872"
     CDS             10836..12230
                     /gene="mapZ"
                     /locus_tag="VVE42_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HEU3073086.1"
                     /translation="MSKKRRNRHKKEGQEPQFDFDEAKELTVGQAIRKNEEVEAGVLP
                     EDSILDKYVKQHRDEIEADKFATRQYKKEEFVETQSLDDLIQEMREAVEKSEASSEEV
                     PSSEDILLPLPLDDEEQGLDPLLLDDENPTEMTEEVEEEQNLSRLDQEDSEKKSKKGF
                     ILTVLALVSVIICVSAYYVYRQVARSTKEIETSQSTTANQSDVDDFNTLYDAFYTDSN
                     KTALKNSQFDKLSQLKTLLDKLEGSREHTLAKSKYDSLATQIKAIQDVNAQFEKPAIV
                     DGVLDTNAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSSSQTSSSSSS
                     PASSNTTSEPKPSSSNETRSSRSEVNMGLSSAGVAVQRSASRVAYNQSAIDDSNNSAW
                     DFADGVLEQILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGY
                     FVGNGAGHADDLDY"
     gene            12306..13730
                     /gene="gndA"
                     /locus_tag="VVE42_000873"
     CDS             12306..13730
                     /gene="gndA"
                     /locus_tag="VVE42_000873"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEU3073087.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPHLDKGDILIDGGN
                     TFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVADVLEEIS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSRKDDEGQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVHASKMLPKPAAFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            13742..14431
                     /locus_tag="VVE42_000874"
     CDS             13742..14431
                     /locus_tag="VVE42_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU3073088.1"
                     /translation="MGKRILLLEKERNLAHFLSLELQKEQYRVDLVEEGQKALSMALQ
                     TDYDLILLNVNLGDMMAQDFAEKLSRTKPASVIMILDHWEDLQEELEVVQRFAVSYIY
                     KPVLIENLVARISAIFRGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLATLMGSKKVLTREQLLESVWKYESATETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGVGYTMQE"
     gene            14530..15528
                     /gene="cbpJ"
                     /locus_tag="VVE42_000875"
     CDS             14530..15528
                     /gene="cbpJ"
                     /locus_tag="VVE42_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpJ"
                     /protein_id="HEU3073089.1"
                     /translation="MKLLKKMMPIALAVFFFGLLGTSTVFADDSEGWQFVQENGRTYY
                     KKGDLKETYWRVIDGKYYYFDSLSGEMVVGWQYIPFPSKGSTIGPYPNGIRLEGFPKS
                     EWYYFDKNGVLQEFVGWKTLEIKTKDSVGRKYGEKREDSEDKEEKRYYTNYYFNQNHS
                     LETGWLYDQSNWYYLAKTEINGENYLGGERRAGWINDDSTWYYLDPTTGIMQTGWQYL
                     GNNWYYLRSSGAMATGWYQEGTTWYYLDQPNGDMKTGWQYLGNKWYYLRSSGAMVTGW
                     YQDGLTWYYLNAGNGDMKTGWFQVNGKWYYAYSSGALAVNTTVDGYYVNYNGEWVR"
     gene            complement(15646..16878)
                     /locus_tag="VVE42_000876"
     CDS             complement(15646..16878)
                     /locus_tag="VVE42_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU3073090.1"
                     /translation="MKLFWTNNIYRQLLLNSCFSSFGDSIFYLAIINYVAQYNFAPLA
                     ILLISISEMVPLLSQLFLGILGDFQENRVKHALWIAKIKILLYAILTVFLVLSPFSLV
                     SVIMIVIINLISDTLSYLSAYMMNALYISVIKDDLHDAMGFRQSLMRVVHIVANLAGA
                     FLINVMSIQTISLINTLTFVIAFLGLYVIRHTLYEVEKRIEMSHTALSFKKYFQHLKQ
                     SLAVLLKLKDTVILLFLTTSMIAILDVSPRLIALRFIQQTLAQLSIGQLLALLSIIMS
                     CGAILGNMTSSNLFKNIRFTHLLVFCEISLLALITSILCQAYIVIFMTSFISSTIIGI
                     LSPRLQAAVFAHIPSDKMGTVGSALSTVDILAPSLLSLLALSIASGVSVQLALIFLYL
                     ILIALIFCQWLVKFNTHN"
BASE COUNT         5275 a         3198 c         3993 g         4792 t
ORIGIN      
        1 gaaattttca ttaggtgttg cttctgttat gattggagct gcattctttg ggacaagtcc
       61 ggttcttgca gatagcgtgc agtctggttc cacggcgaac ttaccagctg atttagctac
      121 tgctcttgca acagcaaaag agaatgatgg gcgtgatttt gaagcgccta aggtgggaga
      181 agaccaaggt tctccagaag ttacagatgg acctaagaca gaagaagaac tattagcact
      241 tgaaaaagaa aaaccggctg aagaaaaacc aaaagaggat aaacctgcag ctgctaaacc
      301 tgaaacacct aagacggtaa cccctgaatg gcagacggta gagaaaaaag aacaaaaggg
      361 aacagtcact atccgagaag aaaaaggtgt ccgctacaac caactatcct caactgctca
      421 aaatgataac gcaggtaaac cagccctgtt tgaaaagaag ggcttgaccg ttgatgccaa
      481 tggaaatgca actgttgatt taaccttcaa agaagattct gaaaagggca aatcacgctt
      541 tggtgtcttc ctgaaattta aagataccaa gaacaatgtt tttgtcggtt atgacaagga
      601 tggctggttc tgggaataca aaactccagg taatagcaca tggtataaag gcaaccgtgt
      661 agctgcacca gaaccgggtt ctgtaaaccg tctttctatc actctcaagt cagatggaca
      721 actcaatgcc agcaacaacg atgtcaatct ctttgacaca gtgactctac cagctgcggt
      781 caatgaccat cttaaaaatg agaagaagat ccttctcaag gcaggaactt atggcaatga
      841 ccgtacggtt gtcagcgtta aaacagacaa tcaagaaggc gtaaaagcgg atgatactcc
      901 tgcccagaaa gaaacaggtc cggttgttga cgatagcaag gtgacttatg atacgatcca
      961 gtctaaggtc ctcaaagcag tgattgacca agccttccct cgtgtcaagg aatacacctt
     1021 gaacgggcat actttgccag gacaagtgca acagttcaac caagtcttta tcaataacca
     1081 ccgaatcacc cctgaagtca cttataagaa aatcaatgag acaacagcag agtacttgat
     1141 gaagcttcgc gatgatgctc acttaatcaa tgcggaaatg acagtacgct tgcaagttgt
     1201 ggacaatcaa ttgcactttg atgtgaccaa gattgtcaac cacaatcaag tcactccagg
     1261 tcaaaagatc gatgacgaaa gaaaactact ttcttctatt agtttcctcg gcaatgcttt
     1321 agtctctgtt tctagtgatc aaactggtgc taagtttgat ggggcaacca tgtcaaacaa
     1381 tacgcatgtc agcggagatg atcatatcga tgtaactaat ccaatgaagg atttggctaa
     1441 gggttacatg tatggatttg tttctacaga taagcttgct gctggtgttt ggagtaactc
     1501 tcaaaatagc tatggtggtg gttcgaatga ctggactcgt ttgacagctt ataaagaaac
     1561 agtcggaaat gccaactatg taggaatcca cagctctgaa tggcaatggg aaaaagctta
     1621 taagggcatt gttttcccag aatacacgaa ggaacttcca agtgctaagg ttgttatcac
     1681 tgaagatgcc aatgcagaca agaaagtcga ttggcaagat ggtgccattg cttatcgtag
     1741 cattatgaac aaccctcaag gttgggaaaa agttaaggat atcacagctt accgtatcgc
     1801 gatgaacttt ggttctcaag cacaaaaccc attccttatg accttggatg gtatcaagaa
     1861 aatcaatctc cacacagatg gtcttgggca aggtgttctc cttaaaggat atggtagcga
     1921 aggtcatgac tctggtcact tgaactatgc tgatattggt aagcgtatcg gtggtgtcga
     1981 agacttcaag accctaattg agaaggctaa gaaatatgga gctcatctag gtatccacgt
     2041 taacgcttca gaaacttatc ctgagtctaa atacttcaat gaaaaaattc tccgtaagaa
     2101 tccagatgga agctatagct atggttggaa ctggctagat caaggtatca acattgatgc
     2161 tgcctatgac ctagctcatg gtcgtttggc acgttgggaa gatttgaaga aaaaacttgg
     2221 tgacggtctc gactttatct atgtggacgt ttggggtaat ggtcaatcag gtgataacgg
     2281 tgcctgggct acccacgttc ttgctaaaga aattaacaaa caaggctggc gctttgcgat
     2341 cgagtggggc catggtggtg agtacgactc taccttccat cactgggcag ctgacttgac
     2401 ctacggtggc tacaccaata aaggtatcaa cagtgccatc acccgcttta tccgtaacca
     2461 ccaaaaagat gcttgggttg gggactacag aagttatggt ggtgcagcca actatccact
     2521 gctaggtggc tacagcatga aagactttga aggctggcaa ggaagaagtg attacaatgg
     2581 ctatgtaacc aacttatttg cccatgacgt catgactaag tacttccaac acttcactgt
     2641 aagtaaatgg gaaaatggta caccggtgac tatgaccgat aacggtagca cctataaatg
     2701 gactccagaa atgcgagtgg aattggtaga tgctgacaat aataaagtag ttgtaactcg
     2761 taagtcaaat gatgtcaata gcccacaata tcgcgaacgt acagtaactc tcaacggacg
     2821 tgtcatccaa gatggttcag cttacttgac tccttggaac tgggatgcga atggtaagaa
     2881 acttcctact gataaggaaa agatgtacta cttcaatacg cagactggtg caacaacttg
     2941 gacccttcca agcgattggg caaagagcaa ggtttacctt tacaagctaa ctgaccaagg
     3001 taagacagaa gagcaagaac taactgtaaa agatggtaaa attaccctag atcttctagc
     3061 aaatcaacca tacgttctct atcgttcgaa acaaaccaat cctgaaatgt catggagtga
     3121 aggcatgcac atctatgatc aaggatttaa tagcggtact ttgaaacatt ggaccatttc
     3181 aggcgatgct tctaaggcag aaattgtcaa gtctcaaggg gcaaacgata tgcttcgtat
     3241 tcaaggaaac aaagaaaaag ttagtctcac tcagaaatta actggcttga aaccaaatac
     3301 caggtatgcc gtttatgttg gtgtagataa ccgtagtaat gccaaggcaa gtatcactgt
     3361 gaatactggt gaaaaagaag tgactactta taccaataag tctctcgcgc tcaactatgt
     3421 taaggcttac gcccacaata cacgtcgtga caatgctaca gttaacgata caagttactt
     3481 ccaaaacatg tacgccttct ttacaacagg atcggacgtc tcaaatgtta ctctgacatt
     3541 gagtcgtgaa gctggtgatc aagcaactta ctttgatgaa attcgtacct ttgaaaacaa
     3601 ttcaagcatg tatggagaca agcatgatac aggtaaaggc accttcaagc aagactttga
     3661 aaatgttgct cagggtatct tcccatttgt agtgggtggt gtcgaaggtg tcgaagacaa
     3721 ccgcactcac ttgtctgaaa aacacgatcc atatacacaa cgtggttgga atggtaagaa
     3781 agtcgatgat gttatcgaag gaaattggtc actcaagaca aatggactag tgagccgtcg
     3841 taacttggtt taccaaacca tcccacaaaa cttccgtttt gaagcaggta agacctaccg
     3901 tgtaaccttt gaatacgaag caggttcaga caatacctat gcttttgtag tcggtaaggg
     3961 agaattccag tcaggtcgtc gtggtactca agcaagcaac ttggaaatgc atgaattgcc
     4021 aaatacttgg acagattcta agaaagccaa gaaggtaacc ttcctcgtga caggtgcaga
     4081 aacaggagat acttgggtag gtatctactc aactggaaat gcaagtaata ctcgtggtga
     4141 ttctggtgga aatgccaact tccgtggtta taacgacttc atgatggata atcttcaaat
     4201 cgaagaaatt accctaacag gtaagatgtt gacagaaaat gctctgaaga actacttgcc
     4261 aacggttgcc atgactaact acaccaaaga gtctatggat gctttgaaag aggcggtctt
     4321 taatctcagt caggccgatg atgatatcag tgtggaagaa gcgcgtgcag agattgccaa
     4381 gattgaagcc ttgaagaatg ctttggttca gaagaaaacg gctttggtag cagatgactt
     4441 tgaaagtttg gatgcgccag ctcaaccagg tgaaggtctt gagaatgcct ttgatggcaa
     4501 tgtgtctagt ctatggcata catcttggaa tggtggagat gtaggcaagc ctgcaaccat
     4561 ggtcttgaaa gaaccaactg aaatcactgg acttcgttat gttccacgtg gatcaggttc
     4621 aaatggtaac ttgcgtgatg tgaaacttgt tgtgacagat gagtctggca aggagcatac
     4681 ctttactgca actgattggc cagataacaa taagccaaaa gacattgact ttggtaagac
     4741 aattaaggtt aagaaaattg tccttacagg tactaagact tacggagatg gtggcgataa
     4801 ataccaatct gcagcggaac ttatctttac tcgtccacag gtagcagaaa cacctcttga
     4861 cttgtcaggc tatgaagcag ctttggctaa ggctcagaaa ttaacagaca aagacaatca
     4921 agaggaagta gctagcgttc aggcaagcat gaaatatgcg acggataacc atctcttgac
     4981 ggaaagaatg gtggaatact ttgcagatta tctcaaccaa ttaaaagatt ctgctacgaa
     5041 accagatgct ccaattgtag agaaacctga gtttaaactt agatctttag cttccgttca
     5101 aggtaagacg ccagattata agcaagaaat agatagacca gatagaccag aaacacctga
     5161 acaaatcttg ccagcaacag gtgagagtca atctgacaca gccctcttcc tagcaggtgt
     5221 tagcctagcc ctatctgctc tctttgtagt aaaaacgaag aaagactagt atttagtaaa
     5281 acctcttaac aagattacgg aagcagtctc tatcttttcc aatgaggttt atagtacaga
     5341 aaaagcctga gaagatgtct tctcaggctt ttgttaagca cataaataca atagtgctat
     5401 gacaaaatca cccagaaaaa tctgggtgat aaatgttatg gttgtgctgg ttgaggattc
     5461 tgattttgtt gatcaggggt tgtatttgat tgttgcgtat tattgttagg attggtagtc
     5521 gtactattat ttgtgcttgg agtggttgag ctagactgtg aagttgaact atctgatgat
     5581 gagcttgaac tttcagttga tgggggttgt tgtggagcag gtgagttcca cgtagaacga
     5641 gcaccatttt taaatacgaa ttctccattt ctgtagagcc cctctggtat attccaatcc
     5701 tctggattgc ttccttcaga caggtaggtc atcatagagc ggtaaacttt ggcagcgacc
     5761 gtaaggccat tgcctacaag tggtgtcaga cggttagaat agcctgtcca tacagccatt
     5821 gaatatttac gcgtatagcc agcaaatagt tcgtcaggtg ctacaaattg agaggtcttg
     5881 atgtggtttt caatttcctc gtctgtatag ttagaggttc ctgttttacc agcctgaggg
     5941 agccaagcaa gataggcatt tcgtccagtt ccataactca agactgtttt catcatgtcg
     6001 gtcatcatat aggctgtcgt ttccttcatg gcacgagttc cgacattaga gaactctttt
     6061 tcactcccat cactaaagac gactttatgg atatacattg gtttatagta agttccacca
     6121 tttgcaaagg cagcgtaagc agcagccatc ttttcactac ttgctccata ttttttgtct
     6181 gattcggttg tgttacttga aatggcattt gagtagtgaa tacttgggta gtcgattcct
     6241 agaccattta ggaaagtctt ggcgcggttg agtccgacct tgtttagagt ttccacggct
     6301 gggacgtttc gcgattgttg cagggcgtat tgcaaggtga tgttgccaaa gtagccccta
     6361 tcccagttat aaacaggagt atttgtccca gggtagttat agggctcatc gtgaacgata
     6421 gtagcagttg aatcgtagac accgtactcc aaggcaggag catagtctgt gatcggtttc
     6481 atagttgatc cccagtcgcg gtttgtttct actgcttggt taattccgaa ggaaacatta
     6541 cttgactgat ggcgtgctcc tagctgggca atgactttac cgttagaaac atcaacaatg
     6601 gtagaagcga cttgcaattc atcgtctgga taggcaacgt attcgtctgt attgtaaata
     6661 tcccacagat gtttttgagc ttcttggtct acatttgtgt agacatccat cccagttgtg
     6721 agtaggttat agcctgtttc ttcttcaact tgattgatga cttccttgag gtaattatcc
     6781 atgtaagcag ggtaattact tgctgatttg agactttgta gcccatcagt aattggtgta
     6841 ttgactgctt tctcatactg ttcagcagag atgtagcctt gatttttcat ttcagataag
     6901 accaagtttc ggcggtcttg ggctgcttct ggatgtgaat aggggtcata ttggtttggt
     6961 gcctgaggca ttccagccag caaggctaac tgaggtaaac ttaaattatt gaggtcttta
     7021 ccatagtagt tttgagctgc tgtctgcatt ccatagttcc cattagacat gtagacctta
     7081 tttatatagt aggtcaagat ttcttgcttg gttgcttttt gttctaactg aatcgctaac
     7141 caagcttcct gagccttacg agaaatagtc tggtcggaag tcgaagttga aaagtaagtc
     7201 aacttaatca actgttgggt gagagttgat ccaccttgga gggaattgct ttgcagattg
     7261 cgcaagaaag ctcccaggat acggatggta tcaatccccc tgtggtcgaa gaagcgatgg
     7321 tcttcgatag aaacgattgc cttaaccaaa tctgtgggaa tatcattagc ttgggcattg
     7381 acgcggcgtt cagaacccaa gtcagcaatg agttgatttt tattgtcgta gattttacta
     7441 gaagttgttg caactagttt actctcggat aggctaggag ccttgctaac gtagtagaaa
     7501 aaaactcctc cgcctaagac aatggctgcg ataaccaagc ttaagaagct aatgctcaga
     7561 tacttgatta ggcgcagaat cgttggtttg ttcatcttgt tttaccacct aataaatgtt
     7621 ctttgataac attgagataa ggaatttgag ggaaggcacc agccttgatt tcatatccat
     7681 attctcgaat atattcaagt ggcattgatt tttgtccctt atcttgatga tagaagcgaa
     7741 tcaaatcgaa tgccggcaat aagtaggttt cttgctgaga agaaaagtga agaaggacaa
     7801 agcagattcc ttgttgggca aggacttgtt ccatatgctg aatctgatgt ggatgaaaat
     7861 ttttcatcgg aatcgcacgt ttttgttttg tttccttgac ttcaaagtcg atgtaatatc
     7921 cattataaac gccagaatag tccgtcgttg aagcttgtcg aaaataggct tcaacaatct
     7981 tggcacgact tcgttgtgga tagtccactt gtacgatttg aataggagtt ggtttcttat
     8041 gtataacagc caagccctga gacaaatagt agtcgttggt agcattgatc atcttttcaa
     8101 aagacattcc tcgatttgcg aaatttttgg gttgagaaag agatgtttgt cttttttgtg
     8161 atgaaacttt atgtggatag ttgaccataa ttctccttat tggtacaata acatcgcttt
     8221 attataccat aatttgcaca gaaagggtta aaaatggcta cagctttggt tttaggctat
     8281 tctgcttttg atttgggttt attttctgat aaggatcctc gtcttaaatt gattaaaaaa
     8341 gcgatacgta aagatttaga ggctatggca gcagatgggg tgtcttggct tgtgtttaca
     8401 gggagtttgg gttttgaata ttgggtttta gaggttgctc aggagatgaa aaccgaatac
     8461 ggtttccagt tggccaccat ttttgctttt gaaacccatg gggaaaattg gaatgaaggc
     8521 aatcagatga aactgagtcg ctttaagcag gtagactttg tcaaatatgc ctatcctcgc
     8581 tatgaacaca aggggcagtt aagagattat cagcagtttt tgctggaaaa cacgactagt
     8641 tcctatcttt tttatgatga agaaaatgaa acaaaactag cttattttta ccaaaagatg
     8701 aaaaatcaag aggactattt tataaagaga ttaacatttg atcaattaaa tgaacttgct
     8761 gaaaattttt ccgaaaattg aagctttgac cttgattttt gtttgccttt ttttatataa
     8821 taatactagc aagcgagaat ggagagagac atggcaagta ttattttttc agcgaaagat
     8881 atttttgaac aagagtttgg acgtgaagtc cgtggctata ataaagtaga agttgacgag
     8941 tttttagacg atgtcatcaa ggactatgaa acctatgctg ccttggtcaa gtcacttcgt
     9001 caggaaattg cggatttgaa ggaagaatta actcgtaaac cgaaaccttc accagttcaa
     9061 gcagaacccc ttgaagcggc aattacaagt tctatgacga attttgatat tttgaaacgc
     9121 ctgaatagat tggaaaaaga agtttttggt aaacaaattt tagataactc agatttttaa
     9181 gtagttattt gagatgtgca atttttggat aatcgcgtga ggagaattgc ttctcatgag
     9241 gaaagtccat gctagcacag gctgtgatgc ctgtagtgtt tgtgctaggc gaaaccataa
     9301 gcctagggac gagaaatcgt tacggcagtt gaaatggcta agtccttgga taagccagag
     9361 taggcttgaa agtgccacag tgacggagtc tttctggaaa cagagagagt ggaacgcggt
     9421 aaacccctca agctagcaac ccaaattttg gtcggggcat ggagtacgcg gaaacgaacg
     9481 tagtattctg actgctatca gctagagctg ttagtggtag acagatgatt atcgaaggaa
     9541 gtggtcctag tcacttctgg aacaaaacat ggcttataga aaattgcata taggttgggg
     9601 ctgagaaatt ttctcaacct cattttttaa agtggacata tagaaaggtc ttgcaagact
     9661 gtaacatgaa aaaagaattt aatttaattg caactgtggc agcagggctt gaggctgtcg
     9721 ttggtcgtga agtgcgagag ttgggctacg attgtcaggt tgaaaatgga cgtgttcgtt
     9781 ttcaaggaga cgtgagagct attatcgaaa ccaacctttg gcttcgggca gcagatcgta
     9841 tcaaaattat cgtaggaacg ttcccagcta agacttttga agagctattt cagggagttt
     9901 tcgctttaga ttgggaaaat tatttaccac ttggagctcg gttcccgatt tcaaaagcta
     9961 aatgtgttaa gtccaaactt cacaatgagc ccagtgttca ggctatttct aagaaagctg
    10021 ttgtcaagaa attgcagaaa cactatgctc gcccagaagg ggttcctttg atggagaatg
    10081 gtccagagtt taagattgag gtctctattc tcaaagatgt ggcaactgtc atgattgata
    10141 cgaccgggtc tagcctcttt aaacgtggtt atcgtaccga aaaaggtggc gctcctatca
    10201 aggaaaatat ggcagcagcc attttacaac tttctaactg gtatccagac aagcctttga
    10261 ttgatccgac ctgtggttcg gggactttct gtattgaggc agttatgatt gctagaaaga
    10321 tggcgccagg tcttcgtcgc tcttttgcat ttgaggaatg gaactggatc agcgatcgct
    10381 tgattcaaga agtgcgcaca gaagcggcta aaaaagtaga ccgtgagctt gagctggata
    10441 tcatgggctg tgatattgat gctcgcatgg tggaaattgc taaggccaat gctcaggcag
    10501 ctggtgttgc aggagacatt acttttaagc agatgcgcgt gcaggattta cgttccgata
    10561 aaatcaatgg agtaatcatt tccaatccgc cttatggtga acgtttgtca gatgatgcag
    10621 gggtgaccaa gctctatgct gagatggggc aagtatttgc accgctgaaa acttggagca
    10681 aatttatcct gactagtgat gaagcttttg aaagcaagta tggtagccaa gcagataaga
    10741 agcgtaagtt atacaacgga accttgaaag tggatctata tcaatatttt ggtcagcgtg
    10801 tcaaacggca agaggtaaaa tagaaaggga tactcatgag taaaaaaaga cgaaatcgtc
    10861 ataaaaaaga aggtcaagaa ccgcaatttg attttgatga agcaaaagag ctaacagttg
    10921 gtcaagctat tcgtaaaaat gaagaagtgg aagcaggagt cttgcctgag gattccattt
    10981 tggacaagta tgttaagcaa cacagagatg aaattgaggc ggataagttt gcgactcgtc
    11041 aatacaaaaa agaggagttc gttgaaactc agagtctgga tgatttaatt caagagatgc
    11101 gtgaggctgt agagaagtca gaagcttctt cggaggaagt tccatcttct gaagacatct
    11161 tactaccctt gcctctggac gatgaggagc aaggcttgga tcctctattg ctagatgatg
    11221 aaaatccaac agaaatgact gaagaagtgg aagaggagca aaacctttct cgtctggatc
    11281 aagaggactc agaaaagaaa agtaaaaaag gctttatttt gaccgttttg gcgcttgtat
    11341 cagtaattat ttgtgtcagt gcttattatg tctaccgtca agtggctcgt tcgaccaagg
    11401 aaattgaaac ttctcaatca actacagcca atcaatcgga tgtggatgat tttaatacac
    11461 tttatgacgc cttttacaca gatagcaata aaacggcttt gaaaaatagc cagtttgata
    11521 aactgagtca actcaaaacc ttacttgata agctggaagg tagtcgtgaa catacgcttg
    11581 ccaaatctaa atatgatagt ctagcaacgc aaatcaaggc tattcaagat gtcaatgctc
    11641 aatttgagaa accagctatt gtggatggtg tgttggatac caatgccaaa gccaaatcgg
    11701 atgctaaatt tacggatatt aaaactggaa atacggagct tgataaagtg ctagataagg
    11761 ctatcagtct tggtaagagc cagcaaacaa gtacttctag ctcaagttca agtcaaacta
    11821 gcagctcaag ttcaagtcca gcaagttcaa atacgactag tgagccaaaa ccaagtagtt
    11881 caaatgagac tagaagtagt cgcagtgaag tcaatatggg tctctcgagt gcaggggttg
    11941 ctgttcaaag aagtgccagt cgtgttgcct ataatcagtc tgctattgat gatagtaata
    12001 actctgcctg ggattttgcg gatggtgtct tggaacaaat tctagcgact tcacgttcac
    12061 gtggctatat cactggagac caatatatcc ttgaacgtgt caatatcgtt aacggcaatg
    12121 gttattacaa cctctataag ccagatggaa cctatctctt tacccttaac tgtaagacag
    12181 gctactttgt cggaaatggc gctggtcatg cggatgactt ggactactaa gcagtcgtta
    12241 caaaattctt tcttttcaaa agtaaaaatg ataaaataaa acaaatgaaa caagaggagt
    12301 gtcaaatgac aaaagctaac tttggtgtcg taggtatggc cgtaatgggt cgtaaccttg
    12361 cccttaatat tgaatcacgt ggttacacag ttgctatcta caaccgtagt aaagaaaaaa
    12421 cggaagatgt gattgcttgc catcctgaaa agaattttgt accaagctat gacgttgaaa
    12481 gttttgtaaa ctcaatcgaa aaacctcgtc gtatcatgct gatggttcaa gctggacctg
    12541 gtacagatgc tactatccaa gcccttcttc cacaccttga caagggtgat atcttgattg
    12601 acggaggaaa cactttctac aaagatacca tccgtcgtaa tgaagaattg gcaaactctg
    12661 gtatcaactt tatcggtact ggggtttctg gtggtgaaaa aggtgccctt gaaggtcctt
    12721 ctatcatgcc tggtggacaa aaagaagcct acgaattggt tgcggatgtt cttgaagaaa
    12781 tctcagctaa agcaccagaa gatggcaaac catgtgtgac ttacatcggt cctgatggag
    12841 ctggtcacta tgtgaaaatg gttcacaatg gtatcgagta cggtgatatg caattgatcg
    12901 cagaaagcta tgacttgatg caacacttgc taggcctttc tgcagaggat atggctgaaa
    12961 tctttactga gtggaacaag ggtgaattgg atagctactt gatcgaaatc acagctgata
    13021 tcttgagccg taaagacgat gaaggccaag atggaccaat cgtagactat atccttgatg
    13081 ctgcaggtaa caagggaact ggtaaatgga cgagccaatc atctcttgac cttggtgtac
    13141 cattgtcact gattactgag tcagtgtttg cacgctacat ttcaacttac aaagaagaac
    13201 gtgtacatgc tagcaagatg cttccaaaac cagctgcctt caactttgaa ggagacaagg
    13261 ctgaattgat tgaaaagatc cgtcaagccc tttacttctc aaaaatcatt tcatacgcac
    13321 aaggatttgc tcaattgcgt gtagcctcta aagaaaacaa ctggaacttg ccatttgcag
    13381 atatcgcatc tatctggcgt gatggctgta tcatccgttc tcgtttcttg caaaagatta
    13441 cagatgctta caaccgtgat gcagaccttg ccaaccttct tttggacgag tacttcttgg
    13501 atgttactgc taagtatcaa caagcagtgc gtgatatcgt agctcttgcg gttcaagctg
    13561 gtgtgccagt gccaactttc tcagcagcta ttacttactt tgatagctac cgttcagctg
    13621 accttccggc taacttgatc caagcacaac gtgactactt tggtgctcac acttaccaac
    13681 gtaaagacaa agaaggaacc ttccactact cttggtatga cgaaaaataa gtaggtcagc
    13741 catggggaaa cggattttat tacttgagaa agaacgaaat ctagctcatt ttttaagttt
    13801 ggaactccag aaagagcagt atcgggttga tctggtagag gaggggcaaa aagccctctc
    13861 catggctctt cagacagact atgatttgat tttattgaac gttaatctgg gagatatgat
    13921 ggctcaggat tttgcagaaa aattgagccg aactaaacct gcctcagtca tcatgatttt
    13981 agatcattgg gaagacttgc aagaagagct ggaagttgtt cagcgttttg cagtttcata
    14041 catctataag ccagtcctta tcgaaaatct ggtagcgcgt atttcggcga tcttccgagg
    14101 tcgggacttc attgatcaac actgtagtct gatgaaagtt ccaaggacct accgcaatct
    14161 taggatagat gttgaacatc acacggttta tcgtggtgaa gagatgattg ctctgacccg
    14221 ccgtgagtat gaccttttgg cgacacttat gggaagcaag aaagtattga ctcgtgagca
    14281 attgttggaa agtgtttgga agtatgaaag tgcgaccgag acaaatatcg tagatgtcta
    14341 tatccgctat ctacggagca agcttgatgt taaaggccaa aaaagctaca ttaaaaccgt
    14401 gcgtggtgtt ggttacacca tgcaagaata gaaaagcagt tgcagttgtg taactgcttt
    14461 tttgaggagt ttttatatat tgacatacaa tctagttttt actacaatcg ttttaggagg
    14521 atatataaaa tgaagctttt gaaaaaaatg atgccaatcg cattagcagt ctttttcttt
    14581 ggtttgctag ggaccagtac agtatttgca gatgattctg aaggatggca gtttgtccaa
    14641 gaaaacggaa gaacctacta caaaaagggg gacctcaaag aaacctactg gcgagtgatt
    14701 gatggtaagt actattattt tgattctcta tctggagaga tggttgtcgg ctggcaatat
    14761 atcccgtttc catctaaagg tagtacaatt ggtccttacc caaatggtat cagattagaa
    14821 ggttttccaa agtcagagtg gtactacttc gataaaaatg gagtgctaca agagtttgtt
    14881 ggttggaaaa cattagagat taaaactaaa gacagtgttg gaagaaagta cggggaaaaa
    14941 cgtgaagatt cagaagataa agaagagaag cgttattata cgaactatta ctttaatcaa
    15001 aatcattctt tagagacagg ttggctttat gatcagtcta actggtatta tctagctaag
    15061 acggaaatta atggagaaaa ctaccttggt ggtgaaagac gtgcggggtg gataaacgat
    15121 gattcgactt ggtactattt agatccaaca actggtatta tgcaaacagg ttggcaatat
    15181 ctaggtaata actggtacta cctccgttca tcaggagcaa tggccactgg ctggtatcag
    15241 gaaggtacca cttggtatta tttagaccag ccaaatggtg atatgaaaac aggatggcaa
    15301 taccttggta acaagtggta ctacctccgt tcatcaggag ctatggtaac tggctggtat
    15361 caagatggtt taacttggta ctacctaaat gcaggtaatg gagacatgaa gacaggttgg
    15421 ttccaggtca atggcaaatg gtactatgct tatagctcag gtgctttggc ggtgaatacg
    15481 accgtagatg gctactacgt caactataat ggcgaatggg ttcggtaatg acagggcgct
    15541 tataattata ttagaacact cgcttaatca aagaagtgac tttgttgtac ttgacctgac
    15601 gattggggag attaaaagca tgtgaaatct acacatgctt tttcgttagt tatgagtgtt
    15661 gaacttgact aaccattgac aaaagataag agcaatcaaa ataagataca aaaatatcaa
    15721 tgctaactgc accgaaacgc ccgatgctat ggacagggct aatagggaga gcagggacgg
    15781 ggcgagaatg tctactgtgc tcagagcaga gcccaccgtc cccatcttgt cactggggat
    15841 atgggcaaag acagctgctt gtaggcgagg gctgagaatg ccgataatcg tagaactgat
    15901 gaaactggtc atgaaaatta cgatataggc ttgacaaagg atactagtta ttagagccaa
    15961 tagggaaatc tcacagaaaa ccaagaggtg cgtgaaacgg atatttttaa atagattact
    16021 gctggtcata ttgccaagga tagctccaca agacatgatg atggagagca gggcgaggag
    16081 ttgcccaatg ctcagttgtg ctagtgtctg ttggatgaag cggagggcaa tcagccgagg
    16141 ggacacatcc aagatggcaa tcatactggt cgtcagaaac agtagtatga cggtatcttt
    16201 taacttcagt agcacagcca gcgactgttt aagatgttga aaatatttct taaaactcag
    16261 tgctgtatgt gacatttcaa tccttttttc aacctcatac aaggtatgtc gaataacata
    16321 caggcccaaa aaggcaatga caaaagtcag agtgttgata agggaaatag tttgaatact
    16381 cataacattg ataaggaatg cgccagccag attggcgaca atgtggacaa ccctcatcag
    16441 agactgcctg aaccccatgg catcatgcag gtcgtcctta attacactga tgtagagggc
    16501 gttcatcatg taggcagaca ggtagctcaa ggtgtcagag atgaggttga tgatgacaat
    16561 cataatgact gaaactaatg aaaagggcga caagacgaga aatactgtca aaatagcgta
    16621 gagcaggatt ttgattttgg caatccagag tgcgtgtttg actctatttt cttgaaaatc
    16681 tcctagaatc ccgagaaaga gttgcgatag taggggaacc atctctgaaa tggaaatcag
    16741 taaaatcgct agcggagcga aattgtactg agccacataa ttgataatgg cgaggtagaa
    16801 aatactgtcg ccgaatgatg aaaaacagct gtttagcagc aactgtctat atatgttgtt
    16861 tgtccaaaat aatttcatac ttttctcctt atgtctgcac atcgaatggg aatttattat
    16921 ttaatagaca ccaatatacc tcatgcttcc tattccgaag tcaaacaaat ctgctttacc
    16981 actatctcct tgaaacatca gtaaaccgtg atcatctcgt tcagaacatg ctaaaattac
    17041 ctgttggatt ttttcatcag taatttgttt tgattgtaga cagtggtttg tagtaagcta
    17101 gttattccgt ggcaatatcc tgtcatcatt tttagatgat ggttaaaaac aaattcttga
    17161 tatttactaa taatatcttt gtttccgtca caatcgtaca tacaaagata taagattatt
    17221 ccggaaattc cttcgcacca agaaagttgt aaatttcc
//
LOCUS       DAVGJL010000031        16768 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.031, whole genome shotgun sequence.
ACCESSION   DAVGJL010000031 DAVGJL010000000
VERSION     DAVGJL010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16768
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.031"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(105..1739)
                     /locus_tag="VVE42_000877"
     CDS             complement(105..1739)
                     /locus_tag="VVE42_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073091.1"
                     /translation="MRLKVIKKLVDINILYSSQEANLANLRKKQAKNPGKKVNVSARV
                     LSSYIFSSLLMLICFSNIAIHFPFEEIPIYFSLMVAILLVIAFSTSLTAFYNVFYESK
                     DLVSYRPYAFKESEIIIAKGLSVLLPALTGIVPILAYFLALYIRLAPSLWLGLPLMLL
                     SLTLLFVSVALVMVVAVHFLAQTRVFRKYQSIFSNVMIGIGVLIPLIFIFFLQSTFGS
                     IVDKVRDIPFLLYPLHIFYKIAVEPFSIEAILGLLAWIGLTLFLLYLTKKKVLPRFYD
                     VILLNSEEKVKKERRSKERISTTKKGFFRMVLRYHLTLLGQGTGVVTVLFTSAFLPYL
                     MMIGLISKIRDSQIVPDIHPPYWLPLFFIALFIAVVNNNITSLPSIALSLERENVDFL
                     KSLPFDFARYVKVKFWIIFAVQSFLPILTLLGLSLYLGLPIISMIYLIVAWILASVIL
                     SCHHYFKDVKNLSTNWSSITDLVNRSNGIVAIVLLFIYSAILMALVIGSIFLVQSLSP
                     ILAISLGVGALILLLALAIFGYHYYLSRILAEIEKR"
     gene            complement(1720..2469)
                     /locus_tag="VVE42_000878"
                     /pseudo
     CDS             complement(1720..2469)
                     /locus_tag="VVE42_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            2647..4101
                     /locus_tag="VVE42_000879"
     CDS             2647..4101
                     /locus_tag="VVE42_000879"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEU3073092.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKAQGIQPMADVVL
                     NHKAAADHREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNGFHWHWYHFT
                     GTDYDAKRSKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYDW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKAVTFVDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQEDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGNHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            complement(4191..6809)
                     /gene="alaS"
                     /locus_tag="VVE42_000880"
     CDS             complement(4191..6809)
                     /gene="alaS"
                     /locus_tag="VVE42_000880"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEU3073093.1"
                     /translation="MKQLSSAQVRQMWLDFWATKGHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPRITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLTSPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDPSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPHKNIDTGAGLERLVAVIQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GKIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGQ
                     DVFKLYDTYGFPVELTEEIAEEAGMTVDREGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESVFNYNTSQLSSKLVAIVADNAEVGAVSEGTASLIFAETSFYAEMGGQVA
                     DYGQILDESGKIVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLAIDSNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVGNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAVAATLKAPQVKEVPHKVEGLQ
                     EQLRQLQKENAELKEKAAAAAAGDIFKDAKEVNGHRYIASQVSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIIDGRGGGKPDMAMAGGS
                     NQAKIQELLDAVAGKL"
     gene            complement(6831..7316)
                     /locus_tag="VVE42_000881"
     CDS             complement(6831..7316)
                     /locus_tag="VVE42_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEU3073094.1"
                     /translation="MKTFLVKQKFRLGGERFAIKDDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSQISKEILTLLPRFEIQLRDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEMLESQS
                     H"
     gene            complement(7591..7830)
                     /locus_tag="VVE42_000882"
     CDS             complement(7591..7830)
                     /locus_tag="VVE42_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073095.1"
                     /translation="MKMSTFFKKSFWPTFMIVNQTAILFHLKDGLDRQYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILKK"
     gene            complement(8099..9169)
                     /locus_tag="VVE42_000883"
     CDS             complement(8099..9169)
                     /locus_tag="VVE42_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU3073096.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLTQFTEETGIQVQYETFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIEGIENIGPEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
                     WKPEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDLQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNVAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQNS
                     AYAFINFMLKPENALQNAEYVGYSTPNLPAKELLPEETKEDKAFYPDVETMKHLEVYE
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            complement(9166..9939)
                     /locus_tag="VVE42_000884"
     CDS             complement(9166..9939)
                     /locus_tag="VVE42_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU3073097.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGDDMNSFTGFSWTH
                     FETMFGDGRLMLILAQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLTPSIIAGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            complement(9936..10742)
                     /locus_tag="VVE42_000885"
     CDS             complement(9936..10742)
                     /locus_tag="VVE42_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU3073098.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVTLLISYPTALFLTRLKHRQLWLMLIILPT
                     WINLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDDMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITLGTAIEQNFLTNDNYGMGSTIGVILILTMFITMWVTKERRER"
     gene            complement(10723..11880)
                     /locus_tag="VVE42_000886"
     CDS             complement(10723..11880)
                     /locus_tag="VVE42_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3073099.1"
                     /translation="MKKPIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGTMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIVGEEIG
                     LDFEPEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEERDEENKL"
     gene            complement(12040..12945)
                     /gene="murB"
                     /locus_tag="VVE42_000887"
     CDS             complement(12040..12945)
                     /gene="murB"
                     /locus_tag="VVE42_000887"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEU3073100.1"
                     /translation="MSVREKMLEILEGIDIRFKEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARLVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESK"
     gene            complement(13057..13809)
                     /locus_tag="VVE42_000888"
     CDS             complement(13057..13809)
                     /locus_tag="VVE42_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
                     /protein_id="HEU3073101.1"
                     /translation="MTIHIIITMLLLLAFLIGSIWFAKKKYQINLAVLGLGAVAFFVS
                     SQILEKLVHILILHPQKDGSIALLQDHPLIYIIYGLAMAAFFEETARLVFFKWLEKKR
                     SLEKADALAYGLGHGGLELIFLGLISLLNLYIVLSAVQTQNPQVMQLLSENMLKTIQS
                     LSVWQIYLLGFERILALGFQLLLTVWVYQAVRQKKWIYLLAAYGLHAFFDLAPSLFQV
                     GWLTNPVLVEVILALELVLVAYGTKEIFCKKS"
     gene            complement(13819..14550)
                     /gene="budA"
                     /locus_tag="VVE42_000889"
     CDS             complement(13819..14550)
                     /gene="budA"
                     /locus_tag="VVE42_000889"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEU3073102.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKGSGDQPEIVEVSPDALIPYAAVVPHQAEVIFRQRFEMTD
                     KELKERIESYYDGENLFRSIKIRGEFSHMHVRMIPKSTPDTKFADVATHQPEYSRDNV
                     AGTIVGFWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            14692..15963
                     /locus_tag="VVE42_000890"
     CDS             14692..15963
                     /locus_tag="VVE42_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEU3073103.1"
                     /translation="MKKQSKYKEVVSYLKNGIESERFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFEQALYCKANQLVLTSGTQQALFILSQISFP
                     RQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLEELEGHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRSILNLAAKYDVYIVEDDYLGDLDSKKGQTFHYLDTEEHVIYI
                     KSFSTSLFPALRITALILPNAIKEAFVAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCPLPHYPLHDGLLLDLRQYPKIASLKHSQLGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            complement(15983..>16768)
                     /locus_tag="VVE42_000891"
     CDS             complement(15983..>16768)
                     /locus_tag="VVE42_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU3073104.1"
                     /translation="CNFLASFFLVACQSGSNGSQSAVEAIKQKGKLVVATSPDYAPFE
                     FQSLVDGKNQVVGADIDMAQAIADELGVKLEISSMSFDNVLTSLQTGKADLAVAGISA
                     TDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGTVPESMVKEQL
                     PKAQLTSLTNMGEAVNELQAGKIDAVHMDEPVALSYAAKNAGLAVATVSLKMKDGDAN
                     AVALRKNSDDLKEVVDKVIQKLKDEGTYQSYLEKAASLTEVEE"
BASE COUNT         5036 a         3803 c         3307 g         4622 t
ORIGIN      
        1 ctccctatat ttttgattac caatcttaat catttacaac tacattctaa caaactataa
       61 aagcgtttgt cgaattgaat ttatcaagca agcgaccaac cagctcatct tttttctatt
      121 tctgccaata tgcgtgacag gtaataatga tagccaaaaa tagcaagagc aagcaggagg
      181 ataagagctc ctactcccaa gctgatggca aggatagggg agagagactg aaccaagaat
      241 atactcccaa ttacaagggc catcaggatt gcactataaa taaacaataa aactatggcg
      301 actatgccat ttgaacgatt caccaggtcc gtaatgctac tccaattggt tgacagattt
      361 ttcacatcct taaaataatg gtgacaagaa aggatgacac tggcaaggat ccatgccaca
      421 ataaggtaaa tcatcgaaat gatgggcaag cctagatata gagaaagacc aagtaaagtc
      481 agaattggta aaaaggactg aacagcaaag ataatccaaa atttcacttt cacataacga
      541 gcaaagtcaa agggtaaact cttcagaaaa tcaacatttt ccctctccaa ggacaaggca
      601 attgaaggca ggctggtgat attgttattg acaactgcta taaagagagc tataaaaaac
      661 aagggtaacc agtatggagg atgaatgtct ggaactatct gagaatctcg gattttggaa
      721 atcagaccga tcatcatgag ataaggaagg aaagcacttg taaaaagcac tgtaaccacg
      781 ccagtcccct gtcccaagag ggtgaggtgg tagcgtaaaa ccatgcggaa aaatcccttt
      841 ttagtggttg aaattctctc cttgctgcga cgttcttttt tgaccttctc ctcactatta
      901 agcaggatca cgtcataaaa acgaggaagg accttctttt tggtcagata aagcaggaag
      961 agagttagtc ctatccaagc gagcagaccc aatatggctt ctattgaaaa aggctccact
     1021 gctattttgt aaaagatatg aagaggataa aggagaaatg gaatgtctct aactttgtca
     1081 acaatacttc caaaagtcga ctgaagaaag aagataaata ttaaaggtat gagaactcct
     1141 atcccaatca tcacattcga aaaaatagac tgatactttc tgaagaccct agtctgagcc
     1201 aagaaatgca ctgccactac catcactaga gccacagaga caaataataa ggtcaaggac
     1261 agtagcatca aaggcaaacc cagccataga gaaggagcta gcctaatata gagggctaga
     1321 aaataagcta ggattggtac aattccagtt agagctggca agaggacaga cagtccttta
     1381 gcaattataa tctctgattc tttaaaggca tagggcctat acgataccaa atccttactc
     1441 tcataaaaga cattgtaaaa ggccgttaaa gaagttgaaa aggcaatcac tagtaaaata
     1501 gcaaccatca agctaaaata aataggtatt tcctcaaaag gaaaatgaat ggctatatta
     1561 ctaaaacaga tgagcatcaa gagactggaa aaaatgtaag aacttaggac tctagcggaa
     1621 acatttactt ttttcccagg attcttagcc tgcttctttc gtagattagc cagattagct
     1681 tcttgagatg aataaaggat attgatatca actaattttt taatgacctt gagacgcatc
     1741 cgcaacctcc tcttttctac cagcaagact aaggtagata ctttccaaag actgggctgg
     1801 gtaatctttt ctcaagtcct ctacactacc acaataaatc aaatgcccct ttttcaaaat
     1861 ggcaatccga tcacagactt gctctgccac ctctaggaca tgagtttaaa acaagactgt
     1921 cttccctttt tgtgcatgtt ccttcatcat ctgtttcaaa tcaaaggcag cctggggatc
     1981 caaaccagtc aagggttcat ccaagaccca aatatcggga tcagacaaga gtgctccgat
     2041 gacaaagact ttctgacgca ttccgcgaga aagagcttca ataacctgat agcgattttc
     2101 agcaaaatca aaaacgttca atagcctagc tagactagcc tccaagtcag atctagtcag
     2161 atcataggat gaggcgatca attcccaaaa ttcattggcc gttaagcgta aaaataagtc
     2221 aggcgagtct gctacgtagc caatctttcg tttaatagcc aagcgatttt ccgataactc
     2281 ctgaccgtct accaaaatac gaccactgct gggtgaaatg atactgacta gggattttat
     2341 agtggtcgat tttccagccc cattatgacc aatcaagccc ataatctctc cattttcaat
     2401 ctgcaaattg agattactca aggcctcttt attaccatac aacttactaa cattttgaaa
     2461 ttcaatcatg ggacaactcc tatctttatc tgtattatac atcgctatac tagcactttg
     2521 atacaaaaaa tcaatacttt attttaagta gttaaaatag tttccttcta tcttacgcaa
     2581 acgtttgcgc cgagagatac tttatgatag aataaagaac aagattgaca agtaagagga
     2641 aacatcatgc aaaatcaaac actcatgcaa tactttgaat ggtatctgcc ccacgacggt
     2701 caacactgga cgcgtctggc tgaaaatgct ccacacctag ctcatctggg gatcagtcac
     2761 gtctggatgc caccagcctt caaggcaacc aacgaaaaag atgtcggcta tggggtctat
     2821 gacttatttg acttaggaga gttcaaccaa aaagggactg tccgcaccaa gtatggtttc
     2881 aaagaagact atcttcaagc cattcaagcc cttaaagcac agggaattca acctatggcc
     2941 gatgtagttc tcaaccacaa ggctgctgcc gatcacaggg aagcctttca ggtcatcgaa
     3001 gttgatcctg tagaccgtac agttgaactt ggagaaccct tcaccatcaa tggctggact
     3061 agttttacct tcgatggtcg ccaagatacc tataatggct tccactggca ttggtaccac
     3121 ttcaccggta cagactacga tgccaaacgc agtaaatctg ggatttatct gatccaaggg
     3181 gacaacaagg gctgggctaa cgaggaattg gtcgataacg aaaacggaaa ctacgactac
     3241 ctcatgtatg ccgacctaga ctttaaacat cctgaagtca tccaaaacat ctatgactgg
     3301 gctgattggt tcatggaaac gactggtgta gctggtttcc gtttggatgc cgttaagcat
     3361 attgactctt tctttatgcg caacttcatc cgcgatatga aggaaaaata cggtgacgat
     3421 ttctatgttt ttggtgaatt ttggaaccca gacaaggaag ccaatctgga ctatctcgaa
     3481 aaaacggaag aacactttga ccttgtcgat gttcgtctcc accagaatct ctttgaagcc
     3541 agtcaagctg gcgcaaacta tgaccttcgt ggcattttca cagatagcct ggttgaactc
     3601 aagcctgaca aggctgtgac ttttgtcgac aaccacgata cccaacgagg acaagccctt
     3661 gagtctaccg ttgaagaatg gttcaagcca gcagcctatg ccctcatttt gttacgccaa
     3721 gacggccttc catgtgtctt ttacggagac tactatggga tttcaggcca gtatgctcaa
     3781 gaagatttca aagaaatcct tgaccgcctc ctagccatcc gaaaagactt ggcctatgga
     3841 gaacaaaatg actactttga ccatgctaac tgtatcggtt gggtacgttc aggtgctgaa
     3901 aatcaatccc caatcgcagt ccttatctca aatgaccaag aaaacagcaa gtcaatgttt
     3961 gtcggtcaag aatggactaa tcaaaccttt gtagatttac ttggtaacca ccaaggtcaa
     4021 gttacaattg atgaggaagg ttatggacaa ttccctgtct cagctagatc cgtaagtgtc
     4081 tgggcagtca ataccatcta atagctcata ataaccaagc taggtccaag cggatttggc
     4141 ttttttgtat tcacaaaaag acctacccaa atggatagat ctttacttga ttacaattta
     4201 cctgctactg catccaacaa ttcttggatc ttagcttggt tgcttcctcc tgccatggcc
     4261 atgtctggtt taccaccacc acgtccatcg atgattggtg ctaattcttt gacaaggttt
     4321 cctgcatgaa ggtcttttgt cttgcttgct acaaggacat tgactttgtc accgatagcg
     4381 gcaactagga caagaagatc agagtagtct ttttgtttcc agttatctgc aaaagtacga
     4441 agggcaccgg catcggatac agacacttga ctagcaatgt aacgatgacc gttgacttcc
     4501 ttagcatctt tgaagatatc gcctgcggct gcagctgcgg ctttttcttt caactcagca
     4561 ttttcttttt gaagttgacg aagttgttct tgaagtcctt ctaccttgtg aggtacttcc
     4621 ttgacttgag gtgctttcaa ggttgctgcg acagctttaa gagcatcctc ttgttcacga
     4681 taggcttcaa aggcttcctt accagtcact gccaagatac ggcgagttcc tgaaccgatt
     4741 ccttcttctt tgacaatttt gaagagacca atctcagaag tgttgccaac atgagtacca
     4801 ccacaaagtt caatagagta gtcaccgata gtcacgacac gaacttcctt gccgtatttc
     4861 tcaccaaaga gggccatagc tcccatttct ttagcagtgt caatatccgt ttcaactgtc
     4921 ttcacttcaa gtgcttccca aattttctcg ttaacttgct gttcaatggc acgaagttcc
     4981 tcagcagtta ctgcttggaa gtgggtaaag tcaaagcgaa ggaattcaac ttcgttaaga
     5041 gatcctgcct gtgttgcgtg gtttccaagg atattgtgaa gggcagcgtg aagcaaatga
     5101 gtcgcagtgt ggtttttcat gacacggtga cggcgattgc tatcaattgc caaggtatat
     5161 tcttggttca aggcaagcgg tgcaaggact tcaactgtat gaagggcttg accatttggg
     5221 gctttctgaa cattggtcac agtagccaca atcttacctg actcatccaa gatttgtccg
     5281 tagtcagcta cctgtccacc catttcagca taaaatgacg tttccgcaaa gataagagag
     5341 gcagttcctt ctgaaacagc tcctacttct gcattgtcag caacgatagc taccaattta
     5401 gaagacaatt ggctagtatt gtagttgaag acactttcta cagtgatgtt ttgaagagtt
     5461 tcattttgca tacccattga gccacccttg acagctgacg cacgcgcgcg ttcttgctgt
     5521 tctttcatgg ctgcttcaaa accttcacgg tctacagtca taccagcttc ttcagcaatt
     5581 tcttcagtca attcaactgg gaatccgtat gtatcataga gtttaaagac atcttgacca
     5641 gcaatgacag attgaccttt ttctttcaag tctgccacaa tgccttgggc aaagtgttga
     5701 cctgagtgaa gggtacgggc aaatgattct tcttcgctct taacgatttt ctcaataaag
     5761 tcacgtttct caagcacttc tgggtagtag ctttccatga tttttccaac agttggaacc
     5821 aatttgtaaa ggaaaggctc gttgattccc aatttttgac catgcataga agcacgacga
     5881 agaagacgac gaaggacata accacgacct tcatttcctg gaagggcacc atcaccgatg
     5941 gcaaatgaaa gtgaacggat gtggtcagcg atgaccttga agctcatgtt gtcgccatct
     6001 tggtcataaa ccttaccaga caatttctcg acttcacgga taatcggcat gaagaggtcc
     6061 gtttcaaagt tggtcttagc cccttggata acggccacca aacgctccaa accagcgccc
     6121 gtatcaatgt tcttatgtgg caattccttg tattcgctac gaggaacagc agggtctgcg
     6181 ttaaattgtg acaaaacgat gttccagatt tcaatataac ggtcgttttc aatatcttct
     6241 gcaagcaggc gaagaccgat attttctggg tcaaaggctt ccccacggtc aaagaagatt
     6301 tctgtatctg gtccagaagg tcccgcacca atttcccaga agttgtcctc aattggaatc
     6361 aagtgacttg gatccactcc cacttcaatc cagcggttgt aagaatcttt atcatctgga
     6421 tagtaagtca tgtaaagttt ctcagctggg aagtcaaacc attcagggct tgtcaaaagc
     6481 tcataagccc aagtgatggc ttcatcacgg aagtaatccc cgattgagaa gttacccaac
     6541 atttcaaaca tggtatggtg acgtgcagtc ttccctacgt tctcgatatc gttagtacga
     6601 atagcctttt gggcattggt aatacgtgga ttttcaggga tgatagtccc gtcaaagtat
     6661 ttcttaaggg ttgctacccc agagttgatc cacaaaagag ttgggtcatt tacaggaacc
     6721 aaacttactg atggttctac tgagtgacct ttggtcgccc agaaatcaag ccacatttgg
     6781 cgtacttgtg cactagatag ttgtttcata ttgtctcctt atttacttgt ttaatgtgat
     6841 tggctttcca gcatttccac atagtcaatc gcgacacaga gggaaatgac taggtctgca
     6901 taagcgtctt caagaaccgt tacggtatag gtagaggtca gatggaagag ttccttctta
     6961 atttccgcaa tcaactgatc gcgatcatcc agcaatttga aattcaaatc ccagatattg
     7021 ccctcgatac gaagacctag attatcaaac tcatacttat ctcgccagaa ggtcaacttc
     7081 ttacgaatga caaaactcga gccatcccga agctgaatct caaaacgagg aagcaaggtc
     7141 aagatttctt tactgatctg actgacttgt tcaccagccg catcatagat ggtaaaagtt
     7201 ttgggaatct taaaaaatga tccctccacc tgataggcaa tttctcccct gtcatccttg
     7261 atagcgaagc gttcgcctcc aagacgaaac ttttgtttga caagaaatgt tttcatcaac
     7321 acctccaaaa atcaaaagac aagctcatat cacgaagggc gaaaaaccgc ggtaccacct
     7381 tcattcaatg aacttgtcat tctcttgttc ttatgcaatt gtatgattga gtagcatgac
     7441 ttcctagctt agatggctcg cagcaccgcc atttctctgg actaagacaa gtgaaaatca
     7501 attctcaact ttcttattat aacgtttttt taagcttgcg tcaactggaa atgatctccg
     7561 ttgaattaga ccaattccct acatctctga ttactttttc aggatatatt ttttcttact
     7621 gccatttttc tttttatccc aaattttcat attactaaac acagctacta gaatatttcc
     7681 aaatataaag gtgcctatca cccaatatat ggactcagtt gttaggtatt gtcgatccaa
     7741 gccatccttt aaatggaata gtatagcagt ttggttaaca atcataaagg ttggccagaa
     7801 actttttttg aaaaaagtag acattttcat tatttgttgc cgctttctgt aaggttaata
     7861 ctcaataaaa atcaaaaagc aaactaggaa gctagcctca agctgtactt gagtacggca
     7921 aggcaacgct gacgtggttt gaagagtata ggctaagtat actactaggc aagcaaataa
     7981 gcaaataagc aaataagcaa ataaacaact agaatagaaa aagatagggc tctaaaaact
     8041 gacttctatt ccttaaaaac gaaccagctt gactgattcg tcttcttacg tttatctcct
     8101 acttccgata cattttaaac tgtaggaaga ggtcgctata tttccctgtc catttatggt
     8161 caaatttctc ataaacttct aggtgtttca tggtttcaac atcgggatag aaggccttat
     8221 cttcctttgt ttcctctggg agcaattcct tcgctggtag gtttggtgtt gaatagccga
     8281 catactccgc attttggaga gcattttcag gtttcaacat aaagttgata aaggcatagg
     8341 ctgagttttg gtttttaact gttttgggaa tgaccatatt gtcaaaccaa agattgctgg
     8401 cctctgtcgg taccacataa cgtagatttt catttttttc taacatttgg ctggcttcac
     8461 cagagaaggt cacgccgatt gcaacattat tctgaatcat atagcccttc atctcgtccg
     8521 caacgatagc cttgatattt ggagtcagtt tgtagagctt atccactgtc tcttccaact
     8581 gctgcagatc cttggagttg aggctgtagc cgagggaatt gagtcctagt cccagcacct
     8641 cacgcgcccc atcaaagagc atgatagaat tcttatactc cggcttccaa aggtcatccc
     8701 aatgctcagg cgcttcatct accatggttt cgttgtagac aattcctaag gttccccaga
     8761 agtaagggat ggagaattta ttacctgggt caaaggactg gttgagaaac tctggtccga
     8821 tattttcgat tccttcaatt tttgaataat caagcggaac caagaggtct tcgtccttca
     8881 tcttgttaat catgtattca cttggaatgg caatatcgta ggtcgttcca ccctgcttta
     8941 tcttagtgta catggcttcg ttggagtcaa aagtctcgta ctgaacttga attcctgttt
     9001 cttctgtaaa ctgagtcaag agttcaggat cgatatagtc tccccagtta tagataacca
     9061 atttttgact atctcgacta ttgattttac tatctaaatg agtcgcaatt ccccacaaga
     9121 caaggataat cgctgcaatt cctgctaaaa atgaatagat ttttttcatg cttgctcctc
     9181 cttctcacga gagataaagt aataacctac aactaggata atactaaaga gaaagactag
     9241 agcagacagg gcattgattt ctaaggaaat ccccttgcga gcacgagagt aaatctcgac
     9301 tgatagggtt gaaaagccat ttcctgttac aaagaaggtc acggcaaaat catccagcga
     9361 ataggtgaag gccatgaagt aacctgcaat gatagacgga gtcaggtaag gaagcatgat
     9421 ttccttaaac atctgaaatt gactagctcc caagtcatag gccgcatgaa tcatgtcgcc
     9481 attcatttcc ttgagtcgag gcaagaccat caagaccacg ataggaatgg agaaggccac
     9541 gtgactagat agaacggtca aaaagccaag tgaaaacttg agttgggtaa agagaatcaa
     9601 gaagctagca ccaatcataa cgtcaggcgc aaccatgagg atattattga gtgatagaaa
     9661 ggcttcttgg tatttcttac gagactggta gatgtaaatg gcaccaaaag tcccgataat
     9721 ggtcgctatc aaggctgata ggaaggccaa gaaaaatgtc tgagccaaaa tcagcatgag
     9781 tctcccatct ccaaacatgg tttcaaagtg agtccagcta aaacctgtaa agctattcat
     9841 atcatcacca gcattaaagg catagccaat caagtaaaaa atcggcaggt agaggaccag
     9901 aaagaccagt cccagataaa ggttggcaaa ttttttcatc gttctctcct ttccttagtc
     9961 acccacatgg tgatgaacat ggtcaggatg agaatcacac cgatagttga acccatacca
    10021 tagttgtcat tggttagaaa attctgctca atagccgtcc ccaaggtgat aacgcggttc
    10081 ccaccaatca aacgggtcag catgaagaga ctcaaacttg ggataaagac cgactgaacc
    10141 ccacttcgca caccgttcat agatagaggg aagatgacat gacggaaggt ctcccactta
    10201 gttgcaccaa ggtcataact agcattgatg agattattat ccatgtcgtc taagacattg
    10261 aaaatcggca aaatcataaa ggggagctcg atgtagcttg cgacaaagat aaaggaaaaa
    10321 tcggtaaaaa gcaactgttg tgaaccaatt ccgataaatt ccaagaattg gttaatagag
    10381 ccattttgac caaaaatccc gataaaagca taggctttaa ggagcaaatt gatccaggta
    10441 ggaaggataa tcagcatgag ccagagttga cggtgcttga gacgggtcaa aaagagggcc
    10501 gtcggataac tgataagcag tgtcacaaag gtcacaatgc ctgcataaag cactgagttg
    10561 aaactcattt taagataggt caagttttgt gacgcaaagt aagatttgta attttctaaa
    10621 ctgaactggc cttcgatgtt gaaaaaggat tgaccgaaaa tcaagaccaa gggtgccaat
    10681 acaaagagcg caatccaaag catgtagggt actacaaaga gtttagagct tgttttcttc
    10741 atctctttcc tcctcgattg cattgatcaa acctgcttct tgctcttcga tttctacgta
    10801 ctcctcaata cgagcatcga actcttcttc ggtttcattg agacgcatga tgtggatgtc
    10861 ttctggttca aagtccagac cgatttcctc acccacgata gccttacggg ttgagtggat
    10921 catccattca tttccaagtt cgtcataggc gataatttca taatgaactc cacggaaaag
    10981 ctgggtatcg accttaactt ggagcttgcc ttcttcagga agggtaatgc gcaagtcctc
    11041 tggacgaata acgacctcaa caggttcatt tggcttcatc ccaccatcaa ccgcttcaaa
    11101 gcgtttgccg ttaaattcga ccaagtagtc ctcaatcatg gtacctggca agatgtttga
    11161 ctccccgata aaggtggcaa caaagtggtt gattggctca tcgtagatgt ccacaggggt
    11221 tccagactgg acaatctcgc catcattcat aacgaaaatc cagtcactca tggcaagagc
    11281 ttcttcctga tcgtgagtga caaagacaaa ggtaatgccc aatcgttgtt gtaattcacg
    11341 caattcgtac tgcatgtctg ttctcaattt caagtccagc gctgataaag gctcgtccaa
    11401 caagaccaca cggggttggt tgatgatagc acgggcgatg gccacacgct gacgttgtcc
    11461 tccagaaagt ttgcggatgg aacgtttttc ataaccttcc aactgaacca tcttgagaac
    11521 ttccgctaca cgctgctcga tttctttctt atcaatttta cgcaagcgaa gtggaaaggc
    11581 aacattttca aacacattca tatgtgggaa caaggcatag gattggaaga cagtatgtac
    11641 gtcgcgcttg ttggttggaa tatcattgat acgaacaccg tctagcatga tatctcctgt
    11701 cgtcgcatcc agtaaacctg caataatgtt taggatagtt gatttccccg aaccagatgc
    11761 acctagaagg gtgtagaatt tcccttcttc caactcaaag ttgatgtctt tgagaacctt
    11821 ggtgttgctg tcttcaaaaa ctttagagac gtttttgaat tcgataattg gttttttcaa
    11881 ttggcataaa ttccttcttt ttcatagatt aaccgatcgg ggctctgtca ggtccccact
    11941 acctcttgca gggagtaaaa ccacctgcat acatcttcgc taccgatagg ctttcacccc
    12001 ctttccatga catagcagca gcttttcaaa tacagcttcc tacttgcttt cacccaagat
    12061 ccggacttct ctttcaagcg taatacctga gtgttccttg actttttcga taaccgattg
    12121 gatcaagtcc tcgtagtctt tggccgttcc atctgcgaca ttgatcataa atcctgcatg
    12181 cttttctgac acttctacgc caccgatacg atagcctttc aagccagctt ctgaaattaa
    12241 ctgacctgca aaatgcccga ctggacgctt aaagaccgag ccacaagatg ggtattccaa
    12301 aggttgcttg agttcacgta ggtgcgtcaa gcggtccatt tcctgcttga taacctgatg
    12361 ggttcctggg gctagggcaa atttaactga caagacaact gcaccagact cctgaatagc
    12421 tgaatggcgg taaccaaaag ccaagtcttt agcagacagg gtttcgattt ctccatcctt
    12481 ggtcaagacc ttacaagact gcaagatgtg agcaatctcg ccaccatagg cacccgcatt
    12541 cataaagaca gcaccgccaa cgcttcctgg aataccacaa gcaaactcaa agccagtcaa
    12601 actatgacgg agggcaatgc gagttgtttc aatcaagtta gccccagctt ctgcttcaat
    12661 ggtatagcca tcaacagaaa cgttattgag cttgtcacac aagatgacaa atccacgaat
    12721 cccaccatca cgaacgatga tattgcttgc attgccaaga accatccaag ggatattttc
    12781 ttggttggca aatttcacaa ggcgcgccaa ctcaaaacga tttcgtggaa agaccaaata
    12841 atcagcctct ccacctactt ttgtataact atagctatgc aagggttcct taaaacggat
    12901 atcaattcct tctaagattt caagcatttt ttctcttaca gacatgtcac tcttcctttt
    12961 acaaaattca ttccattata ccatttttag agacatttga cgaccataaa aataccttgt
    13021 ttggattttg cataagaaaa agaggttccc ccctttttat gattttttac aaaagatttc
    13081 cttggttcca taggcaacca gaacgagctc cagtgctaga atcacttcaa ccaagactgg
    13141 atttgtcaac cagcctactt ggaaaagaga tggtgccaga tcaaagaagg catgcaagcc
    13201 ataggctgct aggagataaa tccatttctt ctggcgaaca gcttggtaaa cccaaactgt
    13261 caaaagtaat tggaaaccaa gcgccaagat tcgctcaaaa ccaagcaaat aaatctgcca
    13321 gaccgaaagt gactgaatgg tttttaacat attttcagac agtaattgca taacctgtgg
    13381 attctgagtt tgaactgccg aaagaacgat gtaaagattg agtaaactaa taaggcctag
    13441 aaaaatcaac tccaagccac catgccctag gccataggcc aaggcatctg ccttttccaa
    13501 acttctcttt ttctccaacc atttgaagaa aacaagacga gccgtttcct caaaaaatgc
    13561 tgccatggct aggccataga taatatagat aagcggatga tcttgcaaga gggcaatact
    13621 accgtctttt tgaggatgca aaatcaagat atgcaccagt ttttccaaaa tctgcgaaga
    13681 gacaaagaaa gcgactgcac ccaagcccaa gacagctaga ttaatctggt atttcttttt
    13741 ggcaaaccaa atgcttccta tcagaaaagc tagcaacagc aacatggtaa taatgatatg
    13801 aatggtcatt ttcttctcct attctgcctt ttcaatatct tttttcatct cgtcaacatt
    13861 gaacttagca aacaagtatt gacggtcttg gactgggaaa cgttggtcca actggtcaac
    13921 tgctcccacc tcgataatgc cttccttgat gacaaaatcc atgacatgtc caccgaaggt
    13981 caaatcatct gatatgaagt gcagatggta gcctgccaca ctgaccccat ggaaaatctc
    14041 aggcgtccag aaaccaacaa tagtccccgc aacattgtca cgactatatt ccggttgatg
    14101 ggttgcgaca tcagcaaact tggtatcggg tgttgactta ggaatcatac gcacatgcat
    14161 atgtgaaaat tccccccgaa tcttgataga gcggaaaaga ttttccccat cataataaga
    14221 ctcaattcgt tctttcaatt ccttgtctgt catctcaaag cgctggcgga aaatgacctc
    14281 tgcctgatgc ggtaccactg cagcgtaagg aataagggca tctggtgaca cttccacaat
    14341 ttctggctgg tctcctgacc ctttggcctg ataagccttg ccatccaaga caatcaattc
    14401 cccatcaatg gaatccaagg ttcccaaacc aaggtcacca tgttctagca attctcctac
    14461 tgtcatagta ccaccataaa ggccagccat caaggctcca agggtattgt attgaaataa
    14521 tttcactggt tcctgcacgt tcttatccat tcactttctt gtctgttatt ctataaatct
    14581 tactcctaag tataccacat ttgcccctag atgtgaacga gagaaacgct ctagacattg
    14641 ccaagaagga aaaaaaaggg tacaatgtaa caaaatcaag ggaggtctgg aatgaagaaa
    14701 caaagcaagt acaaagaggt cgtttcctat ctgaaaaatg gtatcgagtc tgaacgattt
    14761 ccgacgggta gtcgcctgcc ttctatccgt caactgagcc ttgactttca ctgcagcaag
    14821 gacaccattc aacgagccct gctggaatta cggcacgaac aatacctcta tgccaagccc
    14881 cagagcggct actatgtcct agaacaaggg caacatcaag acctagaaat cgaggttacc
    14941 gacgaacatg ccagtgccta tgacgatttc cgactctgtg tcaatgaaac cttgattgga
    15001 cgagaaaact acctcttcaa ctactatgac aatcaagaag gattagaaga cctaagacag
    15061 tccattcaca aactcctatt tgagcaagct ctctactgca aggctaacca actagtactg
    15121 acttctggaa cccaacaagc cttatttatc ctctctcaaa tatcctttcc tagacaagcc
    15181 aaggaaatct tggtggaaca gccaacctac catcggatga atcgcctctt gattgcacag
    15241 gggctggact atcaaacgat tgaacgaggc attgatggga ttgacttgga ggagctggaa
    15301 ggccacttca aaacaggaaa aattaaattt ttctacacca ttcctagatt tcactatcct
    15361 ctgggacatt cctattctga gcaagacaaa cgatctattc ttaacttagc tgccaagtat
    15421 gatgtctata tcgtagagga cgattatctg ggtgatttgg actccaagaa gggtcaaacc
    15481 ttccactatc ttgatacaga ggagcatgtc atttatatca agtccttctc gaccagcctt
    15541 tttcctgccc ttcgtattac agcactcatt cttccaaatg ctatcaaaga agcatttgtg
    15601 gcctacaaaa atatcctaga ctacgacagc aacctcatta tgcaaaaggc cctgtcactc
    15661 tatattgaca gtcaattgtt tgaaaaaaat cgtttggctc gcttgaccaa tcatgaatct
    15721 taccaaaaac aaatcgagga aaggataact aaaacacctt gtccccttcc tcattatccc
    15781 ctacacgatg gtttattgct agacctgaga cagtatccta aaatcgccag tctcaaacac
    15841 agtcaactgg gcttggactt ctttgaagag gcctatttaa gcacctgtcc ttatcaattt
    15901 gccaaggtgt ccctagacaa tctggaaaat gttttaaact atttaaaagc agaattggaa
    15961 tgacatccaa ctctgctttt tcttattctt caacttcagt tagacttgct gctttttcaa
    16021 gataactttg gtaagttcct tcatccttaa gtttttggat gaccttgtct accacttctt
    16081 tcaaatcatc actatttttt ctaagtgcaa cggcattggc gtcgccgtcc ttcatcttca
    16141 agctgacagt tgcgacagct aagccagcgt ttttagcagc ataactaagt gcaacaggct
    16201 catccatatg aacagcatct atttttccag cctgcaattc attgactgct tcacccatat
    16261 tagttaggga agttaattga gcttttggca attgttcctt gaccattgat tctggaacag
    16321 tccctttttg ggctgcaata ttagcacttt ctaggctagt taaatccttg tatttttcca
    16381 catcagcctt acgaaccaag aaactaatct tgttttcata gtatgggatt gaaaaatcaa
    16441 agacttcttt tctctcgtca gtagcactaa ttcctgcaac tgctaggtca gccttaccag
    16501 tttgaagact ggtcaaaaca ttgtcaaaac tcatgcttga gatttccaac ttaaccccaa
    16561 gttcatcagc gatagcctga gccatgtcga tgtctgcacc gactacctgg ttctttccat
    16621 caaccaatga ttgaaattca aagggtgcat agtcaggact ggttgccaca actaatttcc
    16681 ccttttgctt aatggcctca acagctgact gagaaccatt agaacctgac tggcaagcta
    16741 ctaagaagaa acttgcaaga aaattaca
//
LOCUS       DAVGJL010000032        16701 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.032, whole genome shotgun sequence.
ACCESSION   DAVGJL010000032 DAVGJL010000000
VERSION     DAVGJL010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16701
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.032"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            60..1016
                     /locus_tag="VVE42_000892"
                     /pseudo
     CDS             60..1016
                     /locus_tag="VVE42_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002881980.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
     gene            1000..1557
                     /locus_tag="VVE42_000893"
     CDS             1000..1557
                     /locus_tag="VVE42_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU3073105.1"
                     /translation="MKRPLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALEKTQERLHQAGLTNAQLILQGHETLDQFVTKAKAGIFNLGYLPSADKSV
                     ITRPQTTIEALEKLCGLLVKGGRIAIMIYYGHEGGDLERDAVLDFVIQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            1550..1804
                     /locus_tag="VVE42_000894"
     CDS             1550..1804
                     /locus_tag="VVE42_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073106.1"
                     /translation="MDKQYLHEKLDAMRQNFVESTHHERAVGVLDQAHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKEQKEIFESCCKKD"
     gene            1816..3870
                     /locus_tag="VVE42_000895"
     CDS             1816..3870
                     /locus_tag="VVE42_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEU3073107.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFREAEEADVTAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLLWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHTFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VTFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLLSLIALTFVLFVIRFIMIYGYY
                     AYRTRRLKKKLNKYMKDMFLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLVLPHLSDEEEESKDYLMHIAILNEVTLELEKELEDTRNKLPLYAAIDNYH
                     GRIENLILSQENQDDQEDWAALKLLILSIESDGLEQAYEEGNISNRAYRVYQRYLKNI
                     EQGINRKLASRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKDKEQSRLRALDYDQIAE
                     LYLANTEMIIESLENLKGVYRRSLISFMQESRLRETAIISSGAFVERVINRVKPNNID
                     EMLRGYYLERKLIFEYEEKRLITTKYAKKLRQNVNNLENYSLKEAANTLPYDMVELVR
                     RN"
     gene            4049..4909
                     /locus_tag="VVE42_000896"
     CDS             4049..4909
                     /locus_tag="VVE42_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEU3073108.1"
                     /translation="MKKWWKELIDKPLLKAFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLGFMKDVLPPSLYEGVEKIAREVLTQPSTGLLSFSVLSAL
                     WSFSKSMNFLQKAFNKAYGVEKSRGLISHQMLSLLVSFGLQLLFAFALFLILFGQMIL
                     ALLAHYWTKDGIIYQALQGLAGPLIYALLFAILVMLYYFLPNISNRKISYTLPGSAFV
                     LLVILGLLTLFSSYLNYYVHHLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASMQ
                     SLKDPVFKKD"
     gene            5120..5830
                     /gene="ccdA2"
                     /locus_tag="VVE42_000897"
     CDS             5120..5830
                     /gene="ccdA2"
                     /locus_tag="VVE42_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEU3073109.1"
                     /translation="MGHIFFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQAS
                     SGKFSISVTSLLRTLAFIAGISFIFILLGYGAGFLGDLLYASWFQYLTGAIIILLGLH
                     QMEILHFKGLYKEKRLQLQGQGQNGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAA
                     SGGSGAWQGAGLMLVYTLGLALPFLLLALTSSYVLKHFRKLHPYLGILKKVGGFLIIV
                     MGLLVLFGNASILSQLFE"
     gene            5850..6416
                     /locus_tag="VVE42_000898"
     CDS             5850..6416
                     /locus_tag="VVE42_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEU3073110.1"
                     /translation="MKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKT
                     SAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAG
                     DDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAF
                     IDKEGKLVKTHPGFMEKDAILQTLKELA"
     gene            6427..7539
                     /gene="msrB"
                     /locus_tag="VVE42_000899"
     CDS             6427..7539
                     /gene="msrB"
                     /locus_tag="VVE42_000899"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEU3073111.1"
                     /translation="MNDKLKIFLLLGVFFLAITGFYVLLIRNAGQTDASQIEKAAVSQ
                     GGKAVKKTEISKDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDAKQISLKEILLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKENLKNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEP
                     LFSSKDKFESGCGWPSFTQPISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYVD"
     gene            7807..8544
                     /locus_tag="VVE42_000900"
     CDS             7807..8544
                     /locus_tag="VVE42_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU3073112.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWEL
                     IQKQVPDIILTDINMPHLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRQDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSL
                     KSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGEDR"
     gene            8541..10232
                     /locus_tag="VVE42_000901"
     CDS             8541..10232
                     /locus_tag="VVE42_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU3073113.1"
                     /translation="MKRSSLLVRMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGN
                     SQTTISQTSHFIQSYIKKLETTSTGLTQQTDVLAYAENPSQDKVEGIRDLFLTILKSD
                     KDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELVDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYSSSSKMEAMKPYIETGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQL
                     LWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELR
                     EVTRQFNAMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFH
                     DSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINEN
                     VAFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQD
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFGANYQMKIDSRPQKGTKVEIYINRIETS"
     gene            10348..10911
                     /locus_tag="VVE42_000902"
     CDS             10348..10911
                     /locus_tag="VVE42_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEU3073114.1"
                     /translation="MNETQIQRETRQVVEDVLEKTNLKQGALFVLGLSSSEVLGGQIG
                     KESSQEIGELIVETILGILGSRGIHLAVQGCEHVNRALVVERQVAEQFDLEIVSVHPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPILREIGHA
                     HVTALASRPKLIGGARAHYPQDAIRKS"
     gene            10933..16599
                     /locus_tag="VVE42_000903"
     CDS             10933..16599
                     /locus_tag="VVE42_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU3073115.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVNKEII
                     SQEAKDLIQTGKPDRNEVVYGLVYQKDQLPQTGTEASVLTAFGLLTVGSLLLIYKRKK
                     IASVFLVGAMGLVVLPSAEAVDPVATLALASREGVVEMDGYRYVGYLSGDILKTLGLD
                     TVLEETSAQPGEVTVVEVETPQSTTNQEQARTENQVVETEEAPKEEAPKTEESPKEEP
                     KSEIKPTDDTLPKVEEGKEDSAEPAPVEEVGGEVESKPEEKVAVKPESQPSDKPAEES
                     KVEQAGEPVAPREDEKAPVEPEKQPEAPEEEKAVEETPKQEESTPDTKAEETVEPKEE
                     TKTAKGTQEEGKEGQAPVQEVNPEYKVTTGTVEKSTESELDFTTEVVPDDTKYVDEEV
                     VERQGSKGVQVTKTTYETVEVVETDKVLSTTTEVKTPAVPKVVKKGTKPVEGTAVETR
                     EEVIPFATKEQEDDTLKRGTRQVAQEGVNGKKQITETYKTIRGEKTNEAPTVEETVLQ
                     APQDEIIKKGTKGLEKPTLEWVKTDKDVLKKSATASYTLNKPDGVEIKSIKVALKDNT
                     GTVIKEVTVEENNLNATLDNLKYYQGYTLSTTMVYNRGEGEETETLEDKEVQLDLKKV
                     EIKNIKETSLMSVDDAGVETDKSLLTEKPTDVAPLYLRVTTHDNKTTRLAVSSVEEVV
                     VDGKTLYKVTAKAPDLVQRNADNTLSEEYVHYFEKQLPKIGNVYYNFNELITDMQKTP
                     NGEFKLGADLNAVNVPTPNKSYVTAKFTGKLYSEGDKHYTIHNLARPLFAQAENAHIH
                     DINLGNVNINMPWANKTAPLGEMFKKSTIENVKVTGNVVGNNDVTGMVNKLDESDMRN
                     VAFIGNITSVGSAGWWSGGLVSESWRSNTDNSYIDTTIKGNKAKVGGLIAKLNHGADP
                     RDVGARGRLKNSVAKGTIDVRDPQETGGLLHSNWSWGLAENNITMMKVKNGGEILYGS
                     RDAEDDDYFGANWVRNNNAFVNGISEGKQSYSRSSRWKGISEDEAKTRIAKMGITAHE
                     YEITQHLTDKLNRAAFKEDTYKTTQDYKTERELAYRNIEKLQPFYNKEWIINQGNKLT
                     DGSNLMIKEVLSVIGMKNGQFVTDLSDIDKIMIHYADGTKEEKTVTRKADSKVQQIRE
                     YSVEGLGDVVYTPNMVEKDRAQLITDIKVKLDSVQLISPEVRNLMDKRGKAHENTDER
                     RNGYIRNLFLEESLDEVKGNLDKLVKALVENEDHQLNGDEAAMKALVKKVEDNKAKIM
                     LGLAYLNRYYGFKYDEKSMKDIMMFKPDFYGKNVSVLDFLIRVGSREHNIKGNRTLEA
                     YREVIGRTIGIGELNGFLNYNMRLFTEETDINTWYKKAVSHTNYIVEKQSSNLAFANK
                     KYRLYENLNNGEHGKYILPLLTTKKAHMFLISTYNTLAFSAFEKYGKNTEAEREAFKK
                     EIDLRAQEQINYLDFWSRLAADNVRDRLLKSENMVPSAIWDNQEVPGHGWADRMGHNK
                     NGDYAPVREFYGPTGKWHGYNGTGAYAYIFTNPQNSEAVYYIISSMISDFGTSAFTHE
                     TTHINDRMAYLGGWRHREGTDVEAFAQGMLQSPAVSSPNGDYGALGLNMAYKRENDGN
                     QWYNYDPNKLDSRAKIDHYMKNYNEALMMLDHLEADAVIAKNNGDNNKWFKKMDKKWR
                     ENANRNGLVGQPHQWDLLRDLNEEENKKKLTSIDDLVDGNYVTKHNMPGNKHYRAEGF
                     DTAYQTVNMMAGIYGGNTSKSAVGSISFKHNTFRMWGYFGYLDGFLGYASNKYKDAAN
                     KENKGLLGDDFIIKKVSDGKFDSLEVWKKEWFKEVKAKGEKGFVAIEIDGKTITNYAE
                     LRELFDKAVEADLKAGNSNQTVALKEKVYKQLLQKSDGFVGNLFKA"
BASE COUNT         5159 a         3017 c         3769 g         4756 t
ORIGIN      
        1 gaaacagagg aatttatagg aaagtcaggc ttttgagatt gtgggtgatt tttgttataa
       61 tgaaagttat aaaatcttat aataccttga atgattatta tcgaaaactc tttggagaaa
      121 agacttttaa agtccctatt gatgcgggat ttgactgtcc caatcgtgat ggaactgtgg
      181 ctcatggagg ctgtactttt tgtacggttt caggttctgg agatactatt gtagccccag
      241 atgcgcctat ccgtgagcaa ttttataagg aaattgactt tatgcaccgt aagtggccgg
      301 atgttcagaa gtatctggtt tattttcaaa attttaccaa cacccatgaa aaggtggaag
      361 ttattcgaga gcgttatgaa caggctatca atgagccagg tgtggtagga atcaatattg
      421 gaatgcgccc agactgttta ccagacgaaa ccatcgaata tttggctgag ttatcggagt
      481 gcatgcatgt gacggttgaa ttgggcttgt agactactta tgaagcaact tctgacctga
      541 ttaaccgtgc gtattcttat gaattgtagg tggaaacggt caagcgtttg agaaagtatc
      601 ccaagattga gattgtttcc catttgatta atggtttgcc tggtgaaacc catgagatga
      661 tggttgaaaa tgtccgccgt tgtgtcacga ataacgatat tcaagggatt aaactgcatt
      721 tgctccatct tatgacaaat actcgtatgc aacgagatta ccatgaggga cgtttacaat
      781 tgatgagtca ggacgaatat gtcagggtca tctgtgacca actggaaatt attcccaaac
      841 atatcgtcat ccatcgaatt acaggagatg cgcctaggga tatgctgatt ggtcctatgt
      901 ggagcctcaa aaaatgggaa gtcctcaaca gcattgagat ggagatgcga cgtcgtggaa
      961 gtgttcaagg atgcaaggtt gtaaaacagg agtttgaaaa tgaaaagacc acttgagatg
     1021 gcacatgatt ttttggctga ggtcgtgaca aaagaggatg tcgtagtgga tgcgactatg
     1081 ggaaatggtc atgacacgct ttttttagcc aagctagcca agcaagtcta tgcctttgat
     1141 attcagaagc aagccttgga aaagacccaa gagcgtttgc atcaggctgg cttgacaaat
     1201 gcccagttaa tcttgcaagg ccatgagaca ctggaccagt ttgtgacaaa agctaaggca
     1261 gggattttta atctgggcta tttgccgtca gctgataagt ctgtcatcac ccgaccgcag
     1321 acaacgattg aggcattaga aaagctatgt ggcttacttg tcaaaggtgg acgaattgct
     1381 attatgattt actatggtca tgaaggaggc gacctcgaga gagatgctgt cttggatttt
     1441 gtgatccagt tgaaccaaca agagtacaca gctgccattt accgaacttt aaaccaagtc
     1501 aacaacccgc cgtttttagt gatgattgaa aaattagaga gatacagaca tggataaaca
     1561 atacctacac gaaaagctgg atgccatgcg ccagaatttt gttgaatcaa ctcaccacga
     1621 acgagcggtg ggcgtgctag accaagcgca tatgagcaaa aaaatgctta aaatcaagaa
     1681 aaaattagtt gctcttgaaa tggaacggtg ccagagaaaa attgagcaca aagactgttc
     1741 caagattgac caaaaaatca aagagcagaa ggagatattt gaatcctgtt gtaaaaaaga
     1801 ttaaggagga cgtgcgtgga attactgatt tacctcatcc tatttttact ggttttgatt
     1861 gtctcaagta caaccaataa gctcctgccc tttttgcctc tccctttggt gcaaattctt
     1921 ttgggaattg tgattggtct ctttttaccc aataccgact ttcacctcaa tacggagttg
     1981 tttttggccc tggttatcgg acccttgctt ttccgagagg ctgaagaagc agatgttacg
     2041 gctattttaa aacactggcg aatcattgtt tatctcatat ttccagtgat ttttatctcg
     2101 accctgagtt tgggtggctt ggcccatctt ctttggttca gccttccctt ggcagcttgc
     2161 ttggctgttg gggcagccct tggtcctacg gacttggtgg cctttgcctc tctttcggag
     2221 cgttttagct ttcctaagcg cgtgtccaat attcttaagg gcgaaggact cttgaatgat
     2281 gcttctggtt tggtggcttt tcaggtagct ttgacagctt ggacaactgg agctttttct
     2341 ctggggcaag ctagcagttc gctcatcttt tcaatcctag gcggtttttt aattggattt
     2401 ttaacagcca tgaccaaccg cttcctccat accttcttgc taagtgtgcg cgcaacggat
     2461 attgccagtg aacttttatt agaattgagt ttgcctctag tgaccttctt tctggcagaa
     2521 gaagtccatg tttcagggat tattgccgtc gtagttgctg gaattttaaa ggcaagtcgc
     2581 ttcaagaaaa tcacgctcct cgaagcccaa gtggatacgg tgaccgagac ggtctggcat
     2641 acagtgacct ttatgctcaa cggttctgtc tttgtgattt tagggatgga gttggaaatg
     2701 atagcagaac ctatcttgac caatccaatc tataatcctc tacttttatt gctatctctc
     2761 atcgccctta cctttgtcct ctttgtcatt cgttttatta tgatctatgg ctattatgcc
     2821 tatagaaccc gacgcctcaa gaaaaagcta aataagtata tgaaggacat gtttctcttg
     2881 accttttcag gtgttaaggg aacggtgtcg attgctacga ttctcttgat accaagtaat
     2941 ctagaacagg agtatcctct cttgcttttc cttgttgcag gtgtgacgct tgtcagcttt
     3001 ttaacaggtc tcttggtctt gcctcatctt tctgatgaag aggaagaaag caaggattat
     3061 ctcatgcata tcgccatttt gaatgaagta acgctagagt tggaaaaaga gttggaagac
     3121 accagaaata aacttcccct ctatgcggct attgacaatt atcatggacg tattgaaaat
     3181 ctcattttaa gccaagaaaa ccaggatgat caagaagact gggctgcttt gaaactcttg
     3241 attcttagta ttgaaagtga tggtttggaa caggcctatg aagaggggaa cattagcaat
     3301 cgtgcttacc gagtttacca acgttatctg aaaaatatag aacaaggaat caatcgtaaa
     3361 cttgcctcaa gactgaccta ttattttctt gtttccttga ggattttacg ttttctcctt
     3421 catgaagttt ttactcttgg aaagaccttc cgtagctgga aggacaagga gcaaagccgt
     3481 ctccgtgctc ttgattatga ccaaattgca gagctctatc ttgccaatac ggagatgatt
     3541 attgaaagtt tggaaaacct gaagggagtc tacagacgct ctttgattag ttttatgcag
     3601 gagtctcgtc ttcgagaaac agctattatc agcagtggtg cctttgtcga acgggttatc
     3661 aatcgtgtca aacccaacaa tatcgatgaa atgctgagag gctattatct ggagcgcaag
     3721 ttgattttcg aatacgaaga aaaacgattg attacgacta agtatgccaa gaaattacga
     3781 caaaatgtaa ataacttaga gaactattcc ttgaaggaag ctgccaatac cctaccgtat
     3841 gatatggtgg aattggtaag aagaaattag ttaatactct tcgaaaatct cttcaaacca
     3901 cgtcagcgtc gccttaccgt atgtatgtga ctgacttcgt cagtcttatc tacaacctca
     3961 aagcagggct ttgagcagct tgcggctagc tttctagttt gctctttgat tttcattgag
     4021 tataagatta taagtgaagg agtatgacat gaaaaagtgg tggaaagagc tgatagacaa
     4081 acctttatta aaagcttttt tgcattatta tcaagcatca gatagtgagt tgaccagtgt
     4141 cgcagtagcc tactattggt tgatttcgat tttccccctg cttttggtgg tggtcaatat
     4201 cctcccctat tttcagattc ctgtgggaga atttctgggc tttatgaaag acgttctgcc
     4261 cccaagcctc tatgaaggtg tggaaaaaat agccagagaa gttttgacgc aaccttcaac
     4321 aggtttattg agtttttctg ttttatcggc gttatggagt ttttcaaaat ctatgaattt
     4381 cttgcaaaaa gcttttaaca aggcttatgg cgtcgaaaag agtcgtggac ttatttccca
     4441 tcagatgcta agtctcctag tcagttttgg cttgcagctc ctctttgcgt ttgccttgtt
     4501 tttgatctta tttggtcaga tgattttggc tttacttgct cattattgga caaaagatgg
     4561 cattatctat caggctttac aagggttggc aggtcctctg atttacgccc tcctctttgc
     4621 tatcttggtc atgctttatt attttcttcc caatatatct aacaggaaaa ttagctatac
     4681 cctaccaggc agtgcctttg ttctcttagt gattttaggt ttactaactc tgttctctag
     4741 ttatctcaat tactatgtcc atcatttggt ggatgtccga attttaggtt ccgttcttct
     4801 tgttgttatg atgttttggt ttatcttgat tgctaaaatt gttatcctag gcgcggttat
     4861 caatgccagt atgcagagtt tgaaagatcc ggtctttaaa aaagactaac tatttatcta
     4921 ttataaaagc gcatagtatc aagactttat agaaccttga tactatgcgc ttttgtgctt
     4981 tgacagtttt ttgtatagtt tttgtccaag atttctaaat catctgatta aattcctatt
     5041 ttcccctatc attgtccctg ttttttcgaa aatggaagga gtataataaa tctatcaata
     5101 aaatgatagg aggaacttta tgggtcatat tttctttttt ctaagtgtct ttttggcagg
     5161 gattctatcc ttcttttctc cttgtatctt acctttgtta ccggtctata caggggtgtt
     5221 actagatgat aaggatggtg ctcaggcttc tagcggcaaa ttttcaatct cagttactag
     5281 tttattacga actctggcct ttatagcagg aatttccttt atatttattt tgttgggcta
     5341 tggagctggt tttttaggcg atttgcttta tgcttcttgg ttccaatatc ttactggggc
     5401 aattattatc cttcttggtt tgcaccaaat ggagattcta cactttaagg ggctttataa
     5461 ggaaaagagg ctacaactgc aaggacaggg gcaaaatggt aagggctata gtcaggcatt
     5521 tttattgggc ttgaccttta gttttgcttg gacgccttgc gtggggccgg ttctagggtc
     5581 tgttttggcc ttggcggctt caggtggttc aggagcttgg cagggagctg gtctcatgtt
     5641 ggtgtatacg ctgggcttgg cgctaccatt cttgcttcta gctctgacct ctagttatgt
     5701 tttgaaacat ttccgaaaac ttcatcccta tctcggaatc ctcaaaaaag tgggtggttt
     5761 tctcattatt gtgatgggac tcttggttct gtttggaaat gcttcaattt taagtcaatt
     5821 atttgaataa aatggaaagg aatatcaata tgaaaaaatg gcaaacatgt gttcttggag
     5881 caggttcgct cctttgtttg acggcttgtt caggcaagtc cgtgactagt gaacaccaaa
     5941 cgaaagatga aatgaagacg gagcagacag ctagtaaaac aagcgcagct aaagggaaag
     6001 aggtggctga ttttgaattg atgggagtag atggcaagac ctaccgttta tctgattaca
     6061 agggcaagaa agtctatctc aaattctggg cttcttggtg ttccatctgt ctggctagtc
     6121 ttccagatac ggatgagatt gctaaagaag ctggtgatga ctatgtggtc ttgacagtag
     6181 tgtcaccagg acataaggga gagcaatctg aagcggactt taagaattgg tataagggat
     6241 tggattataa aaatctccca gtcctagttg acccatcagg caaacttttg gaaacttatg
     6301 gtgtccgttc ttacccaacc caagccttta tagacaaaga aggcaagctg gtcaaaacac
     6361 atccaggatt catggaaaaa gatgcaattt tgcaaacttt gaaggaatta gcctaggagg
     6421 tgtcttatga atgataagtt aaaaatcttc ttgttgctag gagtattttt tctagccata
     6481 accggtttct atgttctatt gatacgaaat gcagggcaga cagatgcctc gcaaattgaa
     6541 aaggcggcag ttagccaagg aggaaaagca gtgaaaaaaa cagaaattag taaagacgca
     6601 gacttgcacg aaatttatct agctggaggt tgtttctggg gagtggagga atatttctca
     6661 cgcgttcccg gggtgacgga tgccgtttca ggctatgcaa atggtagagg agaaacaacc
     6721 aagtacgaat tgattaacca aacaggtcat gcagaaacgg tccatgtcac ctatgatgcc
     6781 aagcaaattt ctctcaagga aatcctgctt cactatttcc gcattatcaa tccaaccagc
     6841 aaaaataaac aaggaaatga tgtggggacc cagtaccgta ctggtgttta ttacacagat
     6901 gacaaggatt tggaagtgat taaccaagtc tttgatgagg tggctaagaa atacgatcaa
     6961 cctctagcag ttgaaaagga aaacttgaag aattttgtgg tggctgagga ttaccatcaa
     7021 gactatctca agaaaaatcc aaatggctac tgccatatca atgttaatca ggcggcctat
     7081 cctgtcattg atgccagcaa atatccaaaa ccaagtgatg aggaattgaa aaagaccctg
     7141 tcacctgagg agtatgcagt tacccaggaa aatcaaacag aacgagcttt ctcaaaccgt
     7201 tactgggata aatttgaatc cggtatctat gtggatatag caactgggga acctctcttt
     7261 tcatcaaaag acaaatttga gtctggttgt ggctggccta gttttaccca acccatcagt
     7321 ccagatgttg tcacctacaa ggaagataag tcctacaata tgacgcgtat ggaagtgcgg
     7381 agccgagtag gagattctca ccttgggcat gtctttacgg atggtccaca ggacaagggc
     7441 ggcttacgtt actgtatcaa tagcctctct atccgcttta ttcccaaaga ccaaatggaa
     7501 gaaaaaggct acgcttattt actagattat gttgattaat actcttcgaa aatctcttca
     7561 aaccacgtca gcgtcgcctt accgtatgta gggttactga cttcgtcagt tttatctaca
     7621 acctcaaagc tatactttga gctgacttcg tcagttttat ctacaacctc aaagctatac
     7681 tttgagaaac ctgtggctat cttcctagtt tactttttga tttttattga gtataagagg
     7741 tctttcctaa gcagttagag gagaattttg ctatactgat actagtaagt ggtaaaggag
     7801 cagagcatga cctacacaat cttaatcgta gaagatgaat atctggtaag acaaggtttg
     7861 actaaactgg tcaatgtagc agcctacgat atggaaatca tcggtcaggc tgaaaatgga
     7921 aggcaggctt gggaattgat ccaaaagcag gtgccagata tcattttaac cgatatcaac
     7981 atgcctcatc taaatggcat ccagttggcc agtctggtac gagaaaccta tcctcaggtt
     8041 catttggtct ttttaacagg ttacgatgat tttgattatg ccttgtctgc tgtcaaacta
     8101 ggtgtggacg actacctgct caaacccttt tctcgtcagg atattgagga aatgttgggg
     8161 aaaatcaaac aaaaactaga caaggaagag aaagaagagc agttacaaga tttattgact
     8221 aataggtttg aaggaaacat ggcccagaaa atccagtctc atctggctga tagccaattt
     8281 agtttaaagt ctttagccag tgacttaggt tttagtccga cctatctgag ttccttgatt
     8341 aagaaagagt tgggcttgcc ttttcaggat tatctggtga gagaacgtgt taaacaagcc
     8401 aagctcttgc ttttaactac agatctgaag atttatgaga tcgcagagaa ggttggtttt
     8461 gaagatatga actattttac ccaacgtttt aagcagattg caggtgtgac acctcgtcag
     8521 tttaagaagg gagaagaccg atgaagcgtt cttctctttt agttagaatg gttatttcca
     8581 tctttctggt ctttctcatt ctcctagctc tggttggaac tttctactat caatcaagtt
     8641 cttcagccat tgaggccacc attgagggca acagccaaac gaccatcagc cagactagcc
     8701 actttattca gtcttatatc aaaaaactag aaaccacctc gactggtttg acccagcaga
     8761 cggatgttct ggcctatgct gagaatccca gtcaagacaa ggtcgaggga atccgagatt
     8821 tgtttttgac catcttgaag tcagataagg acttgaaaac tgttgtgctg gtgaccaaat
     8881 ctggtcaggt catttctaca gatgacagtg tgcagatgaa aacttcctct gatatgatgg
     8941 ctgaggattg gtaccaaaag gctattcatc agggagctat gcctgttttg actccagctc
     9001 gtaaatcaga tagtcagtgg gtcatttctg tcactcaaga acttgttgat gcaaagggag
     9061 ccaatcttgg tgtgcttcgt ttggatattt cttatgaaac tctggaagcc tatctcaatc
     9121 aactccagtt ggggcagcag ggctttgcct tcattatcaa tgaaaaccat gaatttgtct
     9181 accatcctca acacacagtt tatagttcgt ctagcaaaat ggaggctatg aaaccctaca
     9241 tcgagacagg tcagggttat actcctggtc acaaatccta cgtcagtcaa gagaagattg
     9301 caggaactga ttggacggta cttggcgtgt catcattgga aaagttagac caggttcgga
     9361 gtcagctctt gtggaccttg cttggggcca gtgtcacatc tcttcttgtc tgtctctgct
     9421 tagtgtggtt cagtcttaaa cgctggattg ctcctttgaa ggatttgaga gaaaccatgt
     9481 tggaaattgc ttctggtgct caaaatcttc gtgccaagga agttggtgcc tatgaactga
     9541 gagaagtaac tcgccaattt aatgccatgt tggatcagat tgatcagttg atggtagcta
     9601 ttcgtagcca ggaagaaacg acccgtcagt accaacttca agccctttcg agccagatta
     9661 atccacattt cctctataac actttggaca ccatcatctg gatggctgaa tttcatgata
     9721 gtcagcgagt ggtgcaggtg accaagtcct tggcaaccta tttccgcttg gcgctcaatc
     9781 aaggtaagga cttgatttgt ctctctgacg aaatcaatca tgtccgccag tatctcttta
     9841 tccagaaaca acgctatgga gataagctgg aatacgaaat taatgaaaat gttgcctttg
     9901 ataatttagt cttacccaag ctggtcctac aaccccttgt agaaaatgct ctttaccatg
     9961 gcattaagga aaaggaaggt cagggccata ttaaactttc tgtccagaaa caggattcgg
    10021 gattggtcat ccgtattgag gatgatggcg ttggcttcca agatgctggt gatagtagtc
    10081 aaagtcaact caaacgtggg ggagttggtc ttcaaaatgt cgatcaacgg ctcaaacttc
    10141 attttggagc caattaccag atgaagattg attctagacc ccaaaaagga acgaaagttg
    10201 aaatatatat aaatagaata gaaactagct aactcccagt caattctggg agttttatgc
    10261 gtaattgggc aaactttcta ggttgtctat cttgctaaaa acaagaaaaa acggtatgat
    10321 agaagagtga caacagaggt aacagagatg aacgagacac agattcaaag agaaacacgt
    10381 caggtagtgg aagatgtttt agaaaagacc aatttgaaac agggggccct ttttgtttta
    10441 ggtctatctt ctagtgaggt gctaggtggt cagattggga aagaatccag tcaagagatt
    10501 ggtgagctga tagtggagac tatcttgggt attctaggta gtagaggtat ccatttagca
    10561 gttcaaggtt gtgagcatgt caatcgggct ctcgttgttg aacgtcaggt ggcagagcag
    10621 tttgatctgg aaattgtcag tgtccatcct actcttcatg caggaggttc ggggcagttg
    10681 gcagccttta agtttatgca ggatccagtt gaggttgaat ttatcaaggc tcatgctgga
    10741 ttggatatcg gagacactgc aattggcatg catgtcaagc atgttcaggt tccgattcgc
    10801 cctattttga gagagattgg tcatgcccat gtaacggcac tggctagtcg tccaaaatta
    10861 atcggaggtg cgcgtgcgca ctatccgcaa gacgctatta gaaagtcttg agaatgaata
    10921 aaggagataa aagtgtttaa aaaagaccgt ttttcaattc gtaagattaa gggagttgta
    10981 ggctctgtat ttcttggaag ccttttgatg gctccttctg tagtggacgc agccacctat
    11041 cactatgtaa ataaagagat tatttcacaa gaagctaaag atttaattca gacaggaaag
    11101 cctgacagga atgaagttgt atatggtttg gtgtatcaaa aagatcagtt gcctcaaaca
    11161 gggacagaag catctgtttt gacagctttt ggtttgctga ctgttgggag cttgctttta
    11221 atctacaaga gaaagaaaat tgctagcgtc tttctagttg gagctatggg attggtagtt
    11281 cttcctagtg cagaggctgt agacccagtt gcgaccctag cgctggctag tcgagagggt
    11341 gttgttgaaa tggatggcta tcgctatgtt ggttatctat caggtgacat cctcaaaacg
    11401 cttggcttgg acactgtttt agaagaaacc tcagcacaac ctggagaggt gactgtagtc
    11461 gaagttgaga ctcctcaatc aacaacaaat caggagcaag ctaggacaga aaaccaagta
    11521 gtagagacag aggaagctcc aaaagaagaa gcacctaaaa cagaagaaag tccaaaggaa
    11581 gaaccaaaat cggagataaa acctactgac gacacccttc ctaaagtaga agaggggaaa
    11641 gaagattcag cagaaccagc tccagttgaa gaagtaggtg gagaagttga gtcaaaacca
    11701 gaggaaaaag tagcagttaa gccagaaagt caaccatcag acaaaccagc tgaggaatca
    11761 aaagttgaac aagcaggtga accagtcgcg ccaagagaag acgaaaaggc accagtcgag
    11821 ccagaaaagc aaccagaagc tcctgaagaa gagaaggctg tagaggaaac accgaaacaa
    11881 gaagagtcaa ctccagatac caaggctgaa gaaactgtag aaccaaaaga ggagactaaa
    11941 acagctaagg ggacgcaaga agaaggcaaa gaaggtcaag caccagtaca agaagtaaac
    12001 ccagaatata aagtaacaac aggtacagtt gagaagtcta ccgaaagtga attagatttc
    12061 acaacagaag tagttccgga tgacacaaaa tatgtagatg aagaagttgt tgaacgtcaa
    12121 gggtctaaag gagttcaagt tacgaaaaca acttacgaaa cagtggaagt tgtagagaca
    12181 gacaaagtcc tttctacaac tacagaagtg aaaactcctg ctgttcctaa agtcgttaaa
    12241 aaaggaacaa aaccagttga aggaacagca gtcgaaacaa gagaagaagt cattcctttt
    12301 gctacaaaag aacaagaaga tgacacttta aaacgcggaa caagacaagt agctcaagaa
    12361 ggtgtaaatg gtaagaaaca aattactgaa acctataaaa caattcgcgg agaaaaaaca
    12421 aacgaagctc caacagtaga agaaacagtt ctccaagcac ctcaagatga gattatcaaa
    12481 aaaggcacaa aaggtttaga aaaaccaacc ttagaatggg ttaaaacaga taaagatgtc
    12541 ttgaagaaga gtgcgacagc cagttataca ttgaataaac cagatggggt agaaatcaaa
    12601 tccatcaaag tagctctaaa agataatact ggaacagtta ttaaagaagt aacggtagaa
    12661 gaaaataact taaacgctac tttagacaac ttgaaatatt atcaaggtta tacactatcc
    12721 acaaccatgg tctataatcg aggcgaaggc gaagaaactg aaacgttaga agataaagaa
    12781 gttcagttag accttaaaaa agttgaaatc aaaaacatca aagaaactag cttgatgagt
    12841 gtggatgatg ctggtgttga aacagataag agcttactta ctgaaaaacc gacagacgtt
    12901 gctccacttt acctacgagt gacaactcat gataacaaaa caacaagact ggcagtaagt
    12961 tcagttgaag aagttgttgt tgatggaaaa acattataca aagttactgc caaagcacca
    13021 gacttagttc aacgtaatgc tgataacaca cttagcgaag agtatgttca ttactttgaa
    13081 aaacaacttc caaaaattgg aaatgtgtat tacaacttca acgagttgat tacagatatg
    13141 caaaaaacac cgaatggcga gtttaaactt ggtgcagact tgaatgctgt caatgttcca
    13201 acaccaaata aatcttatgt gactgctaaa tttactggta agttgtatag tgaaggtgat
    13261 aaacactata ctattcacaa cttagcacgt ccattgtttg cgcaagctga aaacgctcat
    13321 attcatgaca tcaacctcgg taatgtaaat attaatatgc cgtgggcgaa caaaacagca
    13381 cctcttggtg aaatgttcaa gaagtcaact attgaaaacg tcaaagtaac aggtaatgtt
    13441 gtcggtaaca atgacgttac aggtatggtc aacaaactgg acgaatctga tatgcgtaac
    13501 gtagctttca ttggaaatat tacaagtgta ggtagcgctg gatggtggtc tggtggactt
    13561 gtcagtgaaa gctggagaag taacacagat aattcttata ttgataccac catcaaaggt
    13621 aataaagcta aggtaggtgg attaattgct aagttaaatc acggtgctga cccacgagat
    13681 gttggtgcga gaggtcgttt gaagaactct gtcgcaaaag gtactattga tgtgagagac
    13741 ccacaagaaa ctggtggatt gctgcattct aactggtcat ggggtcttgc agaaaacaat
    13801 attaccatga tgaaagtgaa gaatggtgga gaaattttat atggttctcg tgatgcagaa
    13861 gatgatgatt acttcggagc gaattgggtg cgtaataata atgcgttcgt caatggaatc
    13921 agtgaaggga aacaatccta ttctcgttct agtcgctgga aagggatttc tgaagatgaa
    13981 gctaaaactc gtattgccaa aatgggaatt acagctcatg aatacgaaat tacacaacat
    14041 ttgactgata aattgaaccg tgcagcattt aaagaagaca catataaaac aactcaagat
    14101 tacaaaactg aaagagaatt agcgtatcgt aatattgaaa agcttcaacc attctacaat
    14161 aaagaatgga ttatcaacca aggtaacaaa ttaacagacg gttcaaactt aatgattaaa
    14221 gaggtattgt ccgttatagg tatgaagaac ggtcagttcg tgaccgattt gtctgatatt
    14281 gataagatta tgattcacta cgctgatggt acgaaagaag aaaaaactgt cacacgtaaa
    14341 gctgactcaa aagtgcaaca aattcgtgaa tatagtgttg aaggtcttgg tgatgttgtg
    14401 tacacaccaa atatggtcga aaaagataga gcgcaattaa ttactgatat taaggtgaaa
    14461 ttggatagcg tccaattgat ttcaccagaa gtgcgcaatt tgatggataa acgtggcaaa
    14521 gcgcatgaaa acacggatga acgtcgtaat ggctacattc gaaacctatt ccttgaagaa
    14581 agtttggatg aagtgaaagg aaaccttgat aagcttgtga aagctcttgt tgaaaatgaa
    14641 gaccatcaat tgaatggtga tgaagcggca atgaaagctc ttgtcaagaa agttgaagac
    14701 aacaaagcga aaatcatgtt aggtcttgct tatctcaatc gctactatgg attcaaatat
    14761 gacgaaaagt ctatgaagga tattatgatg ttcaaaccgg acttctatgg taagaatgtt
    14821 agtgtcttgg acttcttgat tagagttggt tcaagagaac acaatattaa aggtaacaga
    14881 acgttagaag cttatcgtga agtaattggt agaacgattg gtatcggtga gttaaacggt
    14941 ttcttaaact ataatatgcg tttattcaca gaagaaactg acatcaatac ttggtacaaa
    15001 aaagctgtat cgcataccaa ctatattgtt gaaaaacaat cctcaaacct tgcgtttgcc
    15061 aacaagaaat atcgccttta tgaaaacttg aacaatggtg aacatggtaa atatatttta
    15121 ccacttctca ctacgaagaa agctcatatg ttcttgattt caacctataa tacattagcg
    15181 tttagtgcgt ttgagaaata tggtaagaat acagaagctg aacgtgaagc gtttaagaaa
    15241 gaaatcgatt tacgtgctca agagcaaatt aattacttag acttctggtc aagacttgca
    15301 gctgataatg tacgtgacag attgttaaaa tctgaaaata tggttccgtc tgcaatttgg
    15361 gataaccaag aagttccagg tcatggatgg gctgatagaa tgggtcataa taagaatggt
    15421 gactatgctc cggttcgtga gttctatgga cctacaggta aatggcatgg atacaatggt
    15481 acgggtgcat atgcttatat ctttactaat ccacaaaact cagaagctgt atattatatc
    15541 atatctagta tgatcagtga tttcggtact tctgcattca ctcatgaaac tacacacatt
    15601 aatgaccgta tggcgtatct aggtggatgg cgtcaccgtg aaggtactga cgtagaagca
    15661 tttgcccaag gtatgttaca atcaccggct gtctcaagtc cgaacggcga ctacggtgct
    15721 ttaggtctta acatggctta caaacgtgaa aacgatggta accaatggta caactatgat
    15781 ccaaataaat tagatagtcg tgcgaagatt gaccattaca tgaagaatta caacgaagcg
    15841 ctcatgatgc ttgaccactt agaagctgat gccgttattg cgaagaataa cggagataac
    15901 aacaagtggt tcaagaagat ggataagaaa tggcgtgaga acgctaatcg aaatggatta
    15961 gtgggacaac cacaccaatg ggatttactt cgtgacctaa atgaagaaga gaataagaag
    16021 aaattgacaa gcattgatga ccttgtagat ggaaattatg taacgaagca taatatgccg
    16081 ggtaataaac actatcgtgc tgaagggttt gatacagcgt atcaaactgt aaacatgatg
    16141 gctggtattt acggtggtaa tactagtaag agtgctgttg gttctatctc gttcaaacat
    16201 aacaccttcc gtatgtgggg ttacttcggt tatcttgatg gatttttagg ttatgcatct
    16261 aacaaatata aagacgcggc taacaaagaa aataaaggct tacttggcga cgactttatt
    16321 atcaagaaag tgtctgacgg taaatttgac agcctagaag tttggaagaa agaatggttt
    16381 aaagaagtta aagctaaagg tgaaaaagga tttgttgcta ttgaaattga tggtaaaaca
    16441 atcactaact atgctgaact tcgtgaattg ttcgacaaag ctgttgaagc tgacctgaaa
    16501 gctggtaact ctaaccaaac agttgccctt aaagaaaagg tttataaaca actcttgcag
    16561 aaatcagatg gatttgtagg aaatcttttt aaagcataga ttgtattaaa tagaggctct
    16621 ttgtcaactg tagtgggttg aagtcagcta agctcgagaa aggacaaatt tcgtcctttc
    16681 ttttttgatg ttcaaagcga t
//
LOCUS       DAVGJL010000033        16198 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.033, whole genome shotgun sequence.
ACCESSION   DAVGJL010000033 DAVGJL010000000
VERSION     DAVGJL010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16198
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.033"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            103..3909
                     /gene="spuA"
                     /locus_tag="VVE42_000904"
     CDS             103..3909
                     /gene="spuA"
                     /locus_tag="VVE42_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HEU3073116.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPRNANASLEKTEEKPATEPT
                     TSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQGLWTWDDVEKPSENWPNGALSF
                     KDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNLTGDKSVEKLVPKMNEAWLDQDY
                     KVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVKNPSSAQWPDGTDFTATGKYGRY
                     IDIPLNEAAREFGFLLLDESKQGVDVKIRKENYKFTDLKNHSQIFLKDDDESIYTNPY
                     YVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKHSNITNHLGNKVTITDVAIDEAG
                     KKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWRLKDETYSYDGKLGADLKEEGKQVDLT
                     LWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTWKQTLDSTNKLGITDFTGYYYQY
                     QIERQGKTVLALDPYAKSLAAWNSDDAKIDDVHKVAKAAFVDPAKLGPQDLTYGKIHN
                     FKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEAFIEKLDYLKDLGVTHIQLLPVL
                     SYYFVNELKNHERLSDYASSNSNYNWGYDPQNYFSLTGMYSSDPKNPEKRIAEFKNLI
                     NEIHKRGMGAILDVVYNHTAKVDIFEDLEPNYYHFMDADGTPRTSFGGGRLGTTHHMT
                     KRLLVDSIKYLVDTYKVDGFRFDMMGDHDAASIEEAYKAARALNPNLIMLGEGWRTYA
                     GDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGYPNEGQPAFITGGKRDVNTIFKN
                     LIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSIKKDPSKAENYAEIHRRLRLGNL
                     MVLTAQGTPFIHSGQEYGRTKQFRDPAYKTPVAEDKVPNKSHLLRDKDGNPFDYPYFI
                     HDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKGLIALRQSTDAFRLKSLQDIKDR
                     VHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVNADEKAREFNLGTAFAHLRNAEV
                     LADENQAGSVGIANPKGLEWTEKGLKLNALTATVLRVSQNGTSHESTAEEKPDSTPSK
                     PEHQNEASHPAHQDPAPEARPDSTKPDAKVADAENKPSQATADSQAEQPAQEAQEAQA
                     SSVKEAVRNESVENSSKENIPATPDKQAELPNTGIKNENKLLFAGISLLALLGLGFLL
                     KNKKEN"
     gene            complement(4009..5058)
                     /locus_tag="VVE42_000905"
     CDS             complement(4009..5058)
                     /locus_tag="VVE42_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU3073117.1"
                     /translation="MVELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKVGLD
                     VYGIGEHHRADFAVSAPEIVLAAGAVNTKKIRLTSAVSILSSMDPIRLFQQYATIDAL
                     SNGRSEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLDLLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGLPIVYAIIGGNPRYFKKLIQAYRE
                     IGSEAGHADKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLEQVGPNGAMFVGNPDQVAEKLIRMIEDLGLDRFMLHLPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            5115..5192
                     /locus_tag="VVE42_000906"
                     /pseudo
     CDS             5115..5192
                     /locus_tag="VVE42_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(5330..7138)
                     /gene="glmS"
                     /locus_tag="VVE42_000907"
     CDS             complement(5330..7138)
                     /gene="glmS"
                     /locus_tag="VVE42_000907"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEU3073118.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLDGADNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYLEIKEEYLAGHHFKGQTDTEIAVHLIGKFAEEEGLSVLEAFKKALHIIRGSYAFAL
                     VDSQDPEVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDGNRRERTSYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDEAGQ
                     VVVDPDIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYGMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANYTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQAFDLVHELSIVAQSIESTLSEKET
                     IEAKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLSLLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKDTDDIVLTTVHPYLS
                     PISMVVPTQLVAYFATLHRGLDVDKPRNLAKSVTVE"
     gene            complement(7331..8710)
                     /gene="bglA"
                     /locus_tag="VVE42_000908"
     CDS             complement(7331..8710)
                     /gene="bglA"
                     /locus_tag="VVE42_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEU3073119.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWERDIELLLETGHTVFRTSIQWSRIFPQGCGKVNPQGVDFYRK
                     VFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
                     WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPESPLLPEHFYEPYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWF
                     KELSDNNGF"
     gene            complement(8810..10663)
                     /locus_tag="VVE42_000909"
     CDS             complement(8810..10663)
                     /locus_tag="VVE42_000909"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEU3073120.1"
                     /translation="MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPALLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     NDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDGYHLTGVNPGRDFTAEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            complement(10676..11935)
                     /gene="rseP"
                     /locus_tag="VVE42_000910"
     CDS             complement(10676..11935)
                     /gene="rseP"
                     /locus_tag="VVE42_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEU3073121.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLDQTALPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PETAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPEDSQG
                     RYLLGVQPGVKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIENILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRRKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            complement(11957..12760)
                     /locus_tag="VVE42_000911"
     CDS             complement(11957..12760)
                     /locus_tag="VVE42_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEU3073122.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIIAMLAMHE
                     LLKMRGLETMTMEGLLTLFATFVLTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIIFMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            complement(12769..13527)
                     /locus_tag="VVE42_000912"
     CDS             complement(12769..13527)
                     /locus_tag="VVE42_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEU3073123.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALTKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKDLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEAALQEAILAYNRRHRRFGGV"
     gene            complement(13758..14330)
                     /locus_tag="VVE42_000913"
     CDS             complement(13758..14330)
                     /locus_tag="VVE42_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEU3073124.1"
                     /translation="MNTVKNKQEILEAFRENPDMMAILTIIRDLGLKDSWLAAGSVRN
                     FIWNLLSDKSPFDHETDIGVIFFDPDISYEETLLLEKKLREDFPQYQWELKNQVYMHQ
                     HSPHTAPYTSSRDAMSKYPERCTAVGLRLNEESALELFTPYGLEDILNFQVRPTPHFL
                     ENQERMELYQTRLFKKNWQEKWKNLIFKNT"
     gene            complement(14311..15282)
                     /gene="ruvB"
                     /locus_tag="VVE42_000914"
     CDS             complement(14311..15282)
                     /gene="ruvB"
                     /locus_tag="VVE42_000914"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEU3073125.1"
                     /translation="MGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALD
                     HVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNELEPGDVLFIDE
                     IHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTRAGMLSNPLR
                     ARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRV
                     RDFAQIMGNGVIDDIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSV
                     NIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAKAYEHLGYEYSEK"
BASE COUNT         4926 a         3806 c         3074 g         4392 t
ORIGIN      
        1 ttctcaaaag taagaatttt tttcacaaat caaagaaaac gcttgcatat tgttttacta
       61 catgctatac tgatatagaa atttattgat tggagaatca ttatgagaaa aacaccatct
      121 cacactgaga aaaaaatggt ctacagcatc cgttccctca aaaatggaac tggttctgtc
      181 cttatcggcg caagccttgt cttgcttgcc atggctacac caactatctc atccgacgaa
      241 agtacaccaa ccactaacga acccaacaac agaaatacaa ccacccttgc ccaacctctt
      301 actgatacag cagctggctc tggtaagaac gaaagtgata tttcttcacc tagaaatgca
      361 aacgcttccc tagagaaaac agaagaaaaa cctgcaacag agccaactac ttctactagt
      421 ccagtaacaa ctgaaactaa ggcagaagag cccatcgaag ataactactt ccgtatccat
      481 gtcaaaaaac ttcctgaaga aaacaaggat gctcaaggac tatggacttg ggacgatgtt
      541 gaaaaaccat ctgaaaactg gccaaacgga gctttgtcct tcaaggatgc caagaaagat
      601 gactacggct attacctaga tgtcaaatta aagggagaac aagccaagaa aattagcttc
      661 ctcatcaaca atacagctgg aaaaaatcta accggcgata aatctgtaga aaaactagtt
      721 ccaaaaatga acgaggcttg gttagaccaa gattacaagg ttttctctta cgagccacag
      781 cctgcaggaa ctgttcgcgt caactactac cgcacagatg gcaactatga caagaaatct
      841 ctctggtact ggggagatgt aaaaaatcca agtagcgctc aatggcctga cggaacagac
      901 tttacggcta caggcaaata tggccgctat atcgacattc ctcttaatga agccgcaaga
      961 gaatttggat ttttattact agatgagagc aaacaaggag tcgacgtgaa aatccgtaaa
     1021 gaaaattata agttcacaga tttgaaaaat catagccaaa ttttcctaaa agacgatgat
     1081 gaatcgattt acacaaatcc atactatgtc catgatatcc gtatgacagg agcccaacac
     1141 gtaggcactt ctagcattga aagtagcttt tcaacacttg tcggtgctaa aaaagaagat
     1201 atcctcaaac actccaacat cactaatcac ctaggaaaca aggtaactat taccgatgtt
     1261 gcaatcgatg aagctggtaa gaaagtgacc tacagcggag atttctctga cacaaaacat
     1321 ccttatactg ttagctacaa ttccgaccaa ttcactacca aaacaagctg gcgcctgaaa
     1381 gatgagacat acagctatga tggcaaactg ggagctgacc taaaagaaga aggaaaacaa
     1441 gttgatttga ccctttggtc accaagtgct gataaggttt ctgttgttgt ctacgacaag
     1501 aatgaccctg acaaagtagt tggaactgtc gctctcgaaa aaggggaaag aggaacttgg
     1561 aaacaaactc tagacagcac aaacaaactc ggaatcacag atttcactgg ctactattat
     1621 caataccaaa tcgagcgtca aggtaaaact gttcttgcac tcgatcctta cgctaaatct
     1681 cttgctgctt ggaatagcga cgatgccaaa attgacgatg tccataaagt ggctaaagcc
     1741 gcctttgtag atccagctaa actcggacct caagacttga cttatggtaa gattcacaat
     1801 ttcaagactc gtgaagacgc cgttatctac gaagctcatg tgcgtgactt cacttcagat
     1861 cctgccattg caaaagactt gaccaaacca tttgggactt ttgaagcctt cattgaaaaa
     1921 ctagactatc tcaaagactt gggtgtaacc catatccagc tccttccagt cttgtcttac
     1981 tactttgtca atgaattgaa aaaccatgaa cgcttgtctg actacgcttc aagcaacagc
     2041 aactacaact ggggatatga ccctcaaaac tacttctcct tgactggtat gtactcaagc
     2101 gatcctaaga atccagaaaa acgaatcgca gaatttaaaa acctcatcaa cgaaatccac
     2161 aaacgtggta tgggagctat cctagatgtc gtttataacc acacagccaa agtcgatatc
     2221 tttgaagacc tagagccaaa ctactaccac tttatggatg ccgatggcac acctcgaact
     2281 agctttggtg gtggacgctt ggggacaacc caccatatga ccaaacggct cctagttgac
     2341 tctatcaaat acctagttga tacctacaaa gtggatggct tccgcttcga tatgatggga
     2401 gaccatgacg ccgcttctat cgaagaagct tacaaggctg cacgcgccct caatccaaac
     2461 ctcatcatgc ttggtgaagg ttggagaacc tatgccggtg atgaaaacat gcctactaaa
     2521 gctgctgacc aagattggat gaaacatacc gatactgtcg ctgtcttttc agatgacatc
     2581 cgtaacaacc tcaaatctgg ttatccaaac gaaggtcaac ctgcctttat cacaggtggc
     2641 aagcgtgatg tcaacaccat ctttaaaaat ctcattgctc aaccaactaa ctttgaagct
     2701 gacagccctg gagatgtcat ccaatacatc gcagcccatg ataacttgac cctctttgac
     2761 atcattgccc agtctatcaa aaaagaccca agcaaggctg agaactatgc tgaaatccac
     2821 cgtcgtttac gacttggaaa tctcatggtc ttgacagctc aaggaactcc atttatccac
     2881 tccggtcagg aatatggacg tactaaacaa ttccgtgacc cagcctacaa gactccagta
     2941 gcagaggata aggttccaaa caaatctcac ttgttgcgtg ataaggacgg caacccattt
     3001 gactatcctt acttcatcca tgattcttac gattctagcg atgcagtcaa caagtttgac
     3061 tggactaagg ctacagatgg taaagcttat cctgaaaatg tcaagagccg tgactatatg
     3121 aaaggtttga ttgcccttcg tcaatctaca gatgccttcc gacttaagag tcttcaagat
     3181 atcaaagacc gtgtccacct catcactgtc ccaggccaaa atggtgtgga aaaagaggat
     3241 gtagtgattg gctaccaaat cactgctcca aacggcgata tctacgcagt ctttgtcaat
     3301 gcggatgaaa aagctcgcga atttaatttg ggaactgcct ttgcacatct aagaaatgcg
     3361 gaagttttgg cagatgaaaa ccaagcagga tcagtcggaa ttgccaaccc gaaaggactt
     3421 gaatggactg aaaaaggctt gaaattgaat gcccttacag ctactgttct tcgagtctct
     3481 caaaatggaa ctagccatga gtcaactgca gaagagaaac cagactcaac cccttccaag
     3541 cctgaacatc aaaatgaagc ttctcaccct gcacatcaag acccagctcc agaagctaga
     3601 cctgattcta ctaaaccaga tgccaaagta gctgatgcgg aaaataaacc tagccaagct
     3661 acagctgatt cacaagctga acaaccagca caagaagcac aagaagcaca agcatcatct
     3721 gtaaaagaag cggttcgaaa cgaatcggta gaaaactcta gcaaggaaaa tatacctgca
     3781 accccagata aacaagctga acttccaaat acaggaatca aaaacgaaaa caaactccta
     3841 tttgcaggaa tcagcctcct tgcgctcctt ggtctcggtt tcttactaaa aaataaaaaa
     3901 gagaactaaa ctagccctcc tatagaaaaa tcccccaagc attatagctc gggggattaa
     3961 tttttgtaca atatttgttg tcctaataaa cttgattagg attttttatt aagcctcttt
     4021 catagcaaaa taagctcgta ctttgggtgc aacttgtgtt ccgaagagtt caatagctct
     4081 cagaacctgg tcatgaggca tagaaccaag cggtagatga agcatgaagc ggtccaatcc
     4141 taaatcctct atcatgcgaa tcaatttttc ggccacctga tctggattgc caacaaacat
     4201 ggcgccattt ggccctacct gctccaaata ttgctcataa cgcaattcct gccagtgcgg
     4261 acggtctttg gaaatagcat ccaccacttg cttagtcgga tggaaataat ctttcaccgc
     4321 ctgctcacca tcttccgcaa tccaccccca agaatgggct cccactttca agtctttgtc
     4381 agcatggccc gcttcgcttc caatctcacg ataagcctga atcaactttt taaaataacg
     4441 tggattacca ccaataatag catatacaat cggtagacca gcctgagcaa tcttcactgt
     4501 tgattcgaca tgaccacctg tagctatcca caagggcaat ttgtcctgaa ctggacgagg
     4561 ataaacttct ttaccagcaa tcgtttgagt caatcgacct tgccagtcta acttggtctt
     4621 ttcattgact aactgaagca agtctaattt ctcatcaaaa agagagtcgt agtctttcaa
     4681 gtcataacca aacagaggga aagattccgt gaaagagccc cttccagcca taatctccga
     4741 tcgtccattt gacaaagcat cgatagtggc atactgttgg aacaaacgaa tcggatccat
     4801 gcttgacaga atgctgactg cactggtcaa acggattttc ttggtattga ctgccccagc
     4861 ggccaggaca atctctgggg ctgatactgc aaaatccgcc cgatggtgct cgccaatccc
     4921 atatacgtcc aaaccaacct tgtcagccag ctcaatctct gccaccaact ggcgaatgcg
     4981 ttcagcatga ctgtaagttt gtccagtccc ttcaagctcc gttatttccc caaatgttga
     5041 aattcccaat tctaccattg tgattctcct tatctatctc tgtacttcaa tttgaaaaat
     5101 tattctaata ctcaatgaaa atcaaagagc aaactaggaa gctagctgca ggctgctcaa
     5161 aacagtgttt tgaggttgtg gatagaactg acgaagtcag taaccatacc tacggcaagg
     5221 cgacgttgac gtggtttgaa gagattttcg aagagtataa cacgaatctt gagtacaagc
     5281 aaccaatttg ctcacaagaa aaaggcctag ataactagac ttttttagct tattctaccg
     5341 ttactgactt ggcaaggtta cgtggtttgt ccacatcgag gccacggtgg agggttgcaa
     5401 agtaagcgac taattgcgtt ggtacgacca ttgaaattgg tgagaggtat ggatgtacgg
     5461 tcgtaaggac gatatcgtcg gtatctttgg ctacattctc ttctgcgata gtgaggactt
     5521 tggcaccacg ggctgcgacc tcttggatat ttccacgagt atggttggca agaactggat
     5581 ctgacaagag agacaagaca ggcgttcctt cttcaatcaa ggcaatggtt ccgtgcttga
     5641 gttctcctgc cgcaaaacct tcacactgga tataagaaat ctctttgagt ttgagacttg
     5701 cttccatggc tacgtagtaa tcttgaccac gtccgatgta aaaggcgtta cgagttgttt
     5761 caagaagttc acgaaccttg gcttcaatgg tttctttctc tgaaagagtt gattcgatag
     5821 actgagctac gattgacaat tcatgaacca ggtcaaaggc ttgcgcttta gcattaccat
     5881 ttgcttctcc gactgctttt gcaaggaagg caagggctgc gatttgcgct gtataggctt
     5941 tagttgatgc cacggcaatt tcaggacctg cgtgaaggag catggtatag ttggcttcac
     6001 gtgagagggt tgaacctgga acgtttgtca ctgttaagct tggaattccc atttcattag
     6061 ccttgaccaa aacttgacga ctatccgctg tttcaccaga ttggctgata aagatgaaga
     6121 gtggtttctt gctgagaagt ggcataccgt agccccactc agatgagatt ccaagttcaa
     6181 ctggtgtatc tgtcaattct tccaacattt tcttagaagc aaatcctgca tggtaagatg
     6241 ttccagctgc aaggatgtag atgcggtctg cgtcttgaac agccttaatg atatctgggt
     6301 ctacgacaac ttgaccagcc tcatctgtgt aggcttggat gagtttccgc ataacagttg
     6361 gttgctcgtc aatttccttg agcatgtagt aagggtaagt tcccttaccg atatctgaca
     6421 agtcaagttc agcagtgtag ctagtacgct cacgacgatt tccatcatag tcttgaactt
     6481 ccacactatc agccttgacg attaccaact cttggtcatg gatttccatg tattggttag
     6541 tttcacgaat catagccatg gcgtctgagc agaccatgtt atagccttct ccaagaccaa
     6601 tcaaaagtgg tgatttattt ttagctacgt agatgacttc aggatcttgt gagtcaacca
     6661 aggcaaaggc ataagaacca cggatgatgt gaagggcttt tttgaaggct tcaagaactg
     6721 agagcccttc ttcttccgca aattttccaa tcaaatgaac ggctatttca gtatctgtct
     6781 gccccttgaa gtggtgacct gcaaggtatt cttccttgat ttcaagatag ttctcaatca
     6841 ccccattatg caccaagaca aaacgttctg tctcagagcg gtgtgggtga gcattgtcct
     6901 cagttggttt tccgtgagta gcccaacgag tatgtccgat accagttgtt ccctcaacac
     6961 cagctgtctt ggcagacaat tctgcaatac gaccaaccgc cttcaccaaa tggttatcag
     7021 caccatctag gacaaaaatt cccgcagaat catagccacg gtattcaagc ttttcaagcc
     7081 cttgaatcaa aatatcagtt gcatttgtgt ttccaacaac accaacaatt ccacacatag
     7141 tatatacgac acaggcaagc tgtgctttct ccttaaaatt ggtatagtct aattcatctt
     7201 ttatagaatc agcaaaaaca gtatatactt gtttctttca cttgtcaaga gtaaaaattg
     7261 gtatagttca aattaagctc ctgtaagcat aaaaactctg accgattggg ataatcagtc
     7321 agagtccttt ttaaaatcca ttattatcgc ttaattcttt gaaccagtgg cctgatttct
     7381 tcagacgacg ttcttgcgtt tccaagtcta attcgaccaa accatagcga tttttatagc
     7441 tgttgagcca tgaccagcag tcaataaagg tccaaatcaa gtagccctta cagttggcac
     7501 catcttcaat ggcacggtga agttcacgaa gatgaccttt tacaaagtca atacggtaat
     7561 catcttgaat cattccatct tgacggaatt tttcttcccc ttcaacaccc ataccattct
     7621 cagtcaacat ccactcaata ttgccataat tttccttgat attttgggcg atgtcataaa
     7681 tcccttgctc ataaatctcc caaccacgat gagaattgat tttacgtcca ggcatcacat
     7741 aaggctcgta aaaatgttct ggtaagagtg gactctctgg atgcttagca aatcgaggag
     7801 ccataacacg caaaggttga tagtagttca caccaaggaa gtccaccgta ttatcacgaa
     7861 tgagttccaa ctcttcctct gtagcatcag gtaaaagacc gtgttcatgc aagatttcta
     7921 ccaactcctg tggataagtc cccaagacag atggatctaa gaaagattgg gcctgaaaaa
     7981 gggccgcaat acgagctgcc ttgacatcag caggatgctg gctacgtgga taagccggtg
     8041 tcaagttgag gacaatccca atcttggaat caggcaaaag ttcatggcaa gccttaacag
     8101 cccggctgct ggccaattgt gtatgatagg ctaccttaac agctgcctct gcatccacct
     8161 tatgtggata atgggcatca taaaaataac caaattctac aggaacgatg ggctcgttaa
     8221 aggtaatcca ttgatccact aaatctccat aagtctcaaa acaaaaacga gcatagtctt
     8281 cataggctga gactgtcgcc ttattttccc aaccatcacc atcctcttga agggcaaaag
     8341 gtaaatcaaa atgatagaga ttgactaaca gacgaattcc tttagcctta atagcctcaa
     8401 agaccttacg ataaaaatcc acaccttgag ggttgacttt tccacagcct tgtggaaaaa
     8461 tccgtgacca ctgaatagaa gtccgaaaga ctgtgtgacc agtctctaac aaaagctcaa
     8521 tatcccgctc ccaattttca taaaaagtcg atgtcttatc tgaaccaatc ccattatagt
     8581 aacgatttgg ctccacttgg aaccagtaat cccagagatt gtctccctta ccgtcaccag
     8641 ctacacgtcc ttctgtctgc ggtccagaag tagaggatcc ccagacaaaa tcctttggaa
     8701 atcttagcat aacatttacc tctttatcta ctcatttctc ccattataca gaaaaaacaa
     8761 ggtaaaaact agttacattt tttccttgtt tttcttctga ttatagtttt tatttcttgc
     8821 ttaggatttc aagcgtttca agcacgttat ctgcatgaac ctcaatggtg tcaccagttg
     8881 ccttgatctt aacttctaca atgccatcgg ccgctttttt accaacagtg atacggattg
     8941 gaagaccaat caagtcacta tcgctaaatt taacaccgac acgttcgtta cggtcatctg
     9001 tcaagacttc ataaccagct cccatcaagc ttgcttcaag tttttctgtc aaggcttgcg
     9061 cttcttcatc cttgacattg acagtaatca aatgcacatc aaatggtgcc aattctttag
     9121 ggaaattgat tccccaagcg taacggtatt caccttttgg cgttttgtta acaaagaggc
     9181 gagcgtgttg ctccatcact gctgaaagaa gacggctgac accgataccg taacatccca
     9241 tgatgattgg cacagcacga ccattttcat ccaaaacatc tgctcccatg cttgctgagt
     9301 agcgagttcc gagtttgaaa atatgaccga tctcaatacc acgcgcaaag ttaaggacac
     9361 cttgtccatc tggggaaatt tcaccctcac gaacttcacg gatatccaca tattctgcag
     9421 taaaatcacg gcctgggttc acaccagtca agtggtagcc atcttcgtta gcaccgacaa
     9481 ctgcattgcg aacatcttgc accttacgat ctgcaataat tttaatattc tctggcaaac
     9541 caactggtcc aagtgaacca aatcctgctt gaacaacatt cgccacttct tcttcgctag
     9601 caacgtcaaa gaaatctgct cccaagtggt ttttcaactt gacttcgttg agttggtcat
     9661 ttccaactag aagggctgca acaagctcac catctgcaat gtagaagagg gttttaatcg
     9721 tttgttcttc tggaacattg aggaaagctg caacttcatc aattgattta acatctggcg
     9781 ttgcaacacg agtaacttct tcttcagcga caacacggtt gcttggtttg tactcgtttg
     9841 ttgccatttc taagttagct gcatagctag actcacttga gtaagcaatg gtatcttcac
     9901 cagagactat ccatttgagc aattctgcct tgatttcttc ttgcacttct gcaggaattt
     9961 cgtcaaatga ggcaactgac ttgtccaaga caacccagcg gtcaaggtct gtacgagcag
    10021 atgtaatggc cataaattct tggctatcct taccacccat ggctccaccg tcaccaataa
    10081 tagccttgaa gtctaaacca ctacgagtga aaatacgctc ataggctgct ttgtactcat
    10141 cataaacact atccaaacta tcatagttag cgtggaaact ataagcatcc ttcatgataa
    10201 actcacgtgt acgaagaagt ccattacgtg ggcgtttttc atcacgatac ttgggctgaa
    10261 tttgataaag gttgagtggc aattgcttgt aagatttaac agaatcacgg acaatagctg
    10321 taaaggtttc ttcgtgagtt ggacctaaga taaagtctga tttttcacgg tttttcagtt
    10381 tgtaaaggtc ttcaccatag gtttcgtaac gacctgattc acgccacaat tctgcactaa
    10441 gaagggctgg agccaacatc tcaacagcac caatcttttc gaattcttgg cgcatgatgt
    10501 ttttagcttt ttcaatcaca cggttggcaa gtggtagata agaataaaca cctgctgaaa
    10561 cttggcgaac ataaccagca cgcaacataa gagcatggct gataacttga gcatcgcttg
    10621 gcatttcgcg aagcgttggg ataggcattt tactttgttt cataatattc ctcgattatc
    10681 taaaaaagag tcgcataatg tcattccaag tcacagcaat catcaagaca accatgatga
    10741 ccactccggc caaggtgaca taggtttcaa tttcttgttt caatggtttg cggcggatgg
    10801 cttctaggat attgagcaca atcttaccac catccaaggc tggaatcgga ataagattaa
    10861 aaatcccaat attgatggaa atcattgcca agaagtacaa gatattctca attccatttt
    10921 tagcagcatc actacttgcc ttaaagatag caacaggtcc acccaacttg ttcaaatccg
    10981 gttggaaaat cagatttttc agagctgaga gaattcggag agctgagtca gcagcagttg
    11041 taaaaccacc tacaaacatg gatagaaaat ctgacttaac ccccggttga acacctagaa
    11101 ggtaacgacc ttgactatct tcgggtgtaa cagtgacttg tttgtcactc cccttttcag
    11161 aaatagtcac atccaaagtc ggtgccgtct tatctttggt ttctgtttcc acagcttgga
    11221 tcaagctttc ccagttgcta acctcatgtg agccaatctt ggtaatttgt gccgtttctg
    11281 gtactcctac cttggccaag gcaccttggg gcatgatatg gaactgattg gtatcaacat
    11341 ctctgacacc accctgcata aagattaaaa cccaaaaaac aacaacacct aagataaaat
    11401 tgttcatagg acctgcaaaa ttggtaatca gtttgcccca gatagtcgca ttttgatatt
    11461 gaacatctaa aggtgcaatc cgaacctcag taccatctgc ttccacaacc gttgcatcgt
    11521 gatccactgc aaatgttttt tcttcttcca gaaccaatcc tttgataaag agcttgtctt
    11581 caaaatcaaa ctgggtcacc tgcataggga gggctgtttg atccaatttt ttacctgaga
    11641 gattgatgcg tttaacctta ccatcatcag caagtgtcaa actaacaggc gttcctgtct
    11701 tgatttcagt tgtatcatca ccccaaccgg ccatgcggac atagccaccc agaggcaaga
    11761 ttcgaatggt ataggccgtt ccatccttgc caatgtgagc aaagatttta ggtcccatac
    11821 cgatggcaaa ttcacgtact aaaatccctg atttcttggc aaagtagaag tgcccgaact
    11881 cgtgcaccac tacaataatc ccaaaaacca gaataaaggt taaaattccg agcatagcgt
    11941 ttcctccgtc ttttgattaa aagagtccaa ataagtgcat gattggaaat acaagcaaca
    12001 tactatcgaa acgatccaaa acaccaccat gtccagggat aaatttccca gaatccttaa
    12061 caccaaaatg acgtttgatc gaactttcta gtaaatcacc aaattgtcca gcaatgctaa
    12121 agaaaatagc aaagactgac atcttgtaaa ttccatatgg aagagcaact gtactgtcaa
    12181 ctatcataaa gataatggtt actaaaattg ctcctaaaat accacccaag gcaccctcaa
    12241 gggttttatt aggcgatacc cttggtgcta actttcgttt cccatagttc atcccaacaa
    12301 gataggcacc actgtctgtc gcccagacga tacacaaggc taagagagcc ttgtccaaac
    12361 ctgcaacacg agcatctagt aaagcattaa atccaaagcc cacgtagaag ctcatagcaa
    12421 gagggaaaac cgcatcctca atcgtataag acttgctaaa aacggtcgtt cctaacatga
    12481 ttgaaatcaa aacactatag gcaaccacat tcccatcaac tggcaaaaaa gtcaggtaat
    12541 tttccagggg aatggtcaat acaaaggttg caaagagggt caagaggccc tccatcgtca
    12601 tggtctctag acctctcatc ttcaaaagtt catgcatggc tagcatggct atgattccga
    12661 ttgctatctg aagcaagagg cccccaatca ttaaaattgg taggaaaata gccagggcaa
    12721 tccctgcaaa caaggttctt ttctgtaaat cctgggtcat atttcctcct aaactcctcc
    12781 aaatcggcga tggcgacgat tataggcaag aatagcttcc tgcaaggccg cttcgtcaaa
    12841 atcaggccat aaggtgtccg taaaataaag ctcactatag gctccctgcc atggaaggaa
    12901 attgctcaaa cgcaattctc cactagtacg gataatcaag tctggatctc gtaagtcctt
    12961 aggcaaatgc tgggtaaaga gatagttacc aatcaattcc tctgtgatgt cacctgggtt
    13021 gattttggca tctaaaacat cctgggaaat caacttaagc gcctgtgtaa tctcagcacg
    13081 tccaccatag ttaagagcaa aattaagaat caatcctgtg ttgttcttag tcaattcctc
    13141 agccttggtt aaagcttcga aggtttgctt aggcaggcgg tctgtctccc caatcatttg
    13201 aatcttaaca ttattcgcat gtagttccgg gacataatta tcataaaact ctactggcaa
    13261 gttcatgata aacttgactt cctgatctgg acgggtccag ttttccgtag aaaaagcata
    13321 gaccgtaata accttgacgc ccagtttgtt ggctgccttg gtcacggttt gcaatgcttc
    13381 catgcccgcc ttatgtccaa aaactcgcgg ttgcatacgt tttttagccc aacggccatt
    13441 gccatccatg atgatgccga tatgagcagg aacctgtgtc ggaacctcta cttccacagc
    13501 cttatctttc ttaaaaaatc caaacatgat cttattccta ttcaaaaatc tatcgtttca
    13561 ttataccata tttccccatt ttcttctatc actaagctat ttattctcag gcaccaagcc
    13621 catttttcaa aaaaataagc cgcctgattg ggcgacttta tttttatagg gagattatta
    13681 tgaaaaagtt ttaggagttt aagttaaggt cttcttaact tatgaactta gtgtacactc
    13741 cctagcttaa agtttcctta agtattttta aaaatcaaat ttttccattt ctcctgccaa
    13801 tttttcttga ataaacgtgt ttgatagagt tccattcgtt cttgattctc taaaaaatga
    13861 ggagttggac gaacttgaaa attcaaaata tcctccagac cgtatggagt aaagagttcc
    13921 aaagcggatt cttcattcaa gcgcagtcca acagccgtac atcgttctgg atacttactc
    13981 atagcatcac gggaactggt atagggagca gtatgagggc tgtgctggtg catatagact
    14041 tgatttttca attcccactg gtactgagga aagtcctctc gcagtttttt ctccagtaat
    14101 aaggtttcct cataagaaat atccggatca aagaaaatca cacctatatc tgtttcatga
    14161 tcaaaagggg atttgtctga caaaagattc caaatgaaat ttctgacaga acctgctgcc
    14221 aaccacgagt ctttcagacc aaggtctcgg atgatcgtca gaatggccat catatctgga
    14281 ttttctctaa aagcctctaa gatttcttgc ttatttttca ctgtattcat aacctaagtg
    14341 ctcatatgcc ttagcagtcg ccacccgtcc agaccgtgtc cgcatgataa aacctttttg
    14401 aatcaagtaa ggctcataca tatcttcaac tgtctcacgc tcttcggcga tattcacaga
    14461 aagagttcct agaccaacag gtcctccact gtacatctca atcatggtac gaaggatttt
    14521 ttgatccaca tagtccaaac cttcatggtc aacatccagc atagtcaaag ccttatcggt
    14581 aataatatca tcaattaccc cattccccat tatctgggca aaatcgcgca cgcgcttgag
    14641 gagacgattg gcaatacgag gggtcccacg actacgtagg gccaactcag atgctgcctc
    14701 atgagtgatt tccatctcaa aaatatctgc cgtccgctcg acaatttctg tcaagtcagc
    14761 atgggcataa tactccatat gacctgtaat cccaaaacgt gcccgtagcg gattggagag
    14821 cataccagcc cgagtcgtcg caccaatcaa ggtaaaaggt ggtaactcca aatgaacact
    14881 acgactaccc tcaccagccc caatcataat atctatgtag aagtcctcca tagcactata
    14941 aagcacctct tccactgaca ttggcaaacg atggatctca tcaataaaaa ggacatcccc
    15001 aggctctaac tcattcaaaa tagctaccag atctccggct ttttcaatga ctggacccga
    15061 cgtttgcttg agattgactc ccagttcatt ggcaataaca aaggccatgg tcgttttccc
    15121 gagacctgga ggcccaaata agagcacatg atccagcgct tcatcccgca tttttgcagc
    15181 ttcaataaag atttgtagct ggtccttgac cttatcctgt ccgatatatt cacgtaaata
    15241 ctgaggacgg agcgtgcgtt ctactaactc ctcatccccc attatctcat tatctaaaat
    15301 tctacccatg gctctattat atcaaaaaac caagccacaa acaaaaaagc cacctgattg
    15361 ggtgactcct aagtttagca cttatgtggt ataatattct acgacactgc tacaccgcct
    15421 gcgaaaggag gtgagatagc ccatgatgga attagtactc aaaactatca tcggaccaat
    15481 tgtggtcggt gtcgttctcg cttagtcgat aaatggctaa acaaggacaa atagtgtcaa
    15541 aaagacccca agcttatttg gtcgtgatct tagggtcttt tctagcctat gatatagaac
    15601 tagtactcaa ttccttttta ttatcccata gttcacgaat tttgtcaaag gtttacattt
    15661 tcttcaaccg ctgtacgaca agacggttaa gattaagaga acgttaggga ttctatcaat
    15721 ttcatagaaa ttttgatttc gtaaacgaag agacaatctt acatgtcact tctcatttaa
    15781 tacgccacta ctagacaagc aaaatcatta ttacagtagt tccagttctt caattaacag
    15841 tcacttacaa tcaaattgag tttgaactag ctgaagcgac cacagaccta tttcttagtt
    15901 atattcgcta aaaaagtccc cgccaattcc ccgaccaaaa tccgaaaaat accgaaaaat
    15961 atcgaaaaat tatttttaga atagtcccca aaagcctgaa atagagctaa aaaactccac
    16021 ctgattgggt ggagttaagg gagattatta tgaaaaagaa aagtttagga ttttattaaa
    16081 taaagttagg aggtctttat ttaataacta catgatacaa gacgaaactt aaaactagct
    16141 taacttttct aaaattttac tattttgcaa aaaatttcta tcaccagcac ctcaccac
//
LOCUS       DAVGJL010000034        16069 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.034, whole genome shotgun sequence.
ACCESSION   DAVGJL010000034 DAVGJL010000000
VERSION     DAVGJL010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16069
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.034"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            67..2232
                     /locus_tag="VVE42_000915"
     CDS             67..2232
                     /locus_tag="VVE42_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEU3073126.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKISKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALSEMKKDGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRVISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQATKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
     gene            2232..2972
                     /locus_tag="VVE42_000916"
     CDS             2232..2972
                     /locus_tag="VVE42_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU3073127.1"
                     /translation="MAKLKIDVNDLHKHYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEKLGMELLEKVGLADKANANPDSLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            3121..4608
                     /locus_tag="VVE42_000917"
     CDS             3121..4608
                     /locus_tag="VVE42_000917"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEU3073128.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYQSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDSEHYIALRQLQAELN
                     EKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATANKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPASFTKDEIRAEKIKVFKNLYHPTD
                     EELKEHFIRGQYRSGKIDGMKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRGVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTDATATGASPEPYEKLIYDVLNNNSTNFSHWDEVGASWKLIDRIEELWA
                     ENGAPLHDYKAGSMGPQASFDLLEKFGAKWTWQPDIAYRQDGRLE"
     gene            complement(4654..5931)
                     /gene="ftsY"
                     /locus_tag="VVE42_000918"
     CDS             complement(4654..5931)
                     /gene="ftsY"
                     /locus_tag="VVE42_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEU3073129.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEDVDPTFTEVEEVS
                     QEEAEVEIVEQAVFQEEEIQDTVEESLDLEPVVEVSQKEVEEFPHSEEGNTEFLETVE
                     ENNSEILEPERPQTEETVQEKYDRSLKKTRTGFGARLNAFFANFRSVDEEFFEELEEL
                     LIMSDVGVQVASNLTEELRYEAKLENAKKPDALRRVIIEKLVELYEKDGSYDESIHFQ
                     DNLTVMLFVGVNGVGKTTSIGKLAHRYKRAGKKVMLVAADTFRAGAVAQLAEWGRRVD
                     VPVVTGPEKADPASVVFDGMERAVAEGIDILMIDTAGRLQNKDNLMAELEKIGRIIKR
                     VVPEAPHETFLALDASTGQNALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAIREEL
                     NIPVKLIGFGEKIDDIGEFNSENFMKGLLEGLI"
     gene            complement(5935..6753)
                     /locus_tag="VVE42_000919"
     CDS             complement(5935..6753)
                     /locus_tag="VVE42_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU3073130.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKETLKAARDRGIKVVLTT
                     GRPLKAMDFFLHELGTDGQEDEYTITFNGGLVQKNTGEILDKTVFSYDDVARLYEETE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFEDLSSQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGIDQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVPEVKAAANVVTPMTNDEEAVAWAIEEYVLKEN"
     gene            complement(6753..7547)
                     /locus_tag="VVE42_000920"
     CDS             complement(6753..7547)
                     /locus_tag="VVE42_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU3073131.1"
                     /translation="MTIKLVATDMDGTFLDGNGRFDMDRLKSLLASYKEKGIYFAVAS
                     GRGFLSLEKLFADVRDDIIFIAENGSLVEYQGQDLYEATMSRDFYLATFEKLKTSPYV
                     DINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEDGEAWVNENVPGVKAMTTGFESIDIVLDYVDKGVAIVELAKKLGITMDQVMAFGDN
                     LNDLHMMQVVGHPVAPENARPEILELAKTVIGHHKDQSVIACMEGL"
     gene            complement(7544..11083)
                     /gene="smc"
                     /locus_tag="VVE42_000921"
     CDS             complement(7544..11083)
                     /gene="smc"
                     /locus_tag="VVE42_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEU3073132.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HIYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKPEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENARKFLDL
                     EGQRKAIYLDVLVAQIKENKAELESTEEELAQVQELLMSYYQKREKLEEENQTLKKQR
                     QDLQAEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLAALEDKRNS
                     LSKEKYDKESSLALLEGNLVQNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSRQLSQKQADQLEKLKEQLATAKEKASQQKDELETAKVQVQK
                     LLADYQAIAKEQEEQKTSYQAQQSQLFDRLDSLKNKQARAQSLENILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDVHYQTALEIALGASSQHIIVEDEESATKAIDFLKR
                     NRAGRATFLPLTTIKARTISSQNQDVIAASAGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDTVEHARAAARKVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIFIKPELEQLQK
                     EIAEEEASLGSEEATLKTLQDQMARLSELLEAIKSQGEQARIQEQGLSLAYQQTSQQV
                     EELETLWKLQEEEIDRLSDGDWQADKENCQERLAAIASDKQNLEAEIEEIKSNKNAIQ
                     ERYQNLQEELAQARLLKTELQGQKRYEVADIERLGKELDNLNIEQEEIQRMLQEKVDN
                     LEKVDTELLSQQAEESKTQKTNLQQGLIRKQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRHLQNQLTDQYQISYTEALEKAHELENLNLAEQEVQDLEK
                     AIRSLGPVNLEAIDQYEEVHNRLDFLNSQRDDILSAKNLLLETITEMNDEVKERFKST
                     FEAIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            complement(11074..11772)
                     /locus_tag="VVE42_000922"
     CDS             complement(11074..11772)
                     /locus_tag="VVE42_000922"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEU3073133.1"
                     /translation="MKELQTVLKNHFEIEFADKKLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFDQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVMIPKVEAGEFEMITD
                     YKTHLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            complement(11955..12026)
                     /locus_tag="VVE42_000923"
     tRNA            complement(11955..12026)
                     /locus_tag="VVE42_000923"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11991..11993),aa:Arg,seq:ccg)
     gene            complement(12090..13076)
                     /locus_tag="VVE42_000924"
     CDS             complement(12090..13076)
                     /locus_tag="VVE42_000924"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEU3073134.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENVAEQLAKGGYFYIMHRFDEAGRIPFIKRMHDQGLIASISVGVKDYEY
                     DFVSQLKADAPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPAKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
     gene            complement(13251..14567)
                     /locus_tag="VVE42_000925"
     CDS             complement(13251..14567)
                     /locus_tag="VVE42_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HEU3073135.1"
                     /translation="MRITDNQHKIIKEKFVEEYPKLSNLLLDRTLESLSQDERIFIFP
                     NDLTHTPDLDKDQKIFETVNQKIKTGNVIGFLGYGQERLTISSRFSDESNDHFLHYLL
                     NKVLYINLTSLDVALSREERLYQLLMYLFPKYLQAAIRKGLYKEYHRFSHNDSNVKGV
                     IVVRNHLKKNLPFTGNIAYTTREFTYDNPLMQLVRHTIEYIKNQKSIGQGVLDNLSTS
                     RENVSEIVRVTPSYKLADRAKITRRNQSKPIRHAYFHEYRNLQELCLMILNQEKHGLG
                     YQDQKIYGILFDVAWLWEEYVYTLLPKGFVHPRNKDKTDGISVFSVGKRKVYPDFYDR
                     ERKIVLDAKYKKLELTEKGINREDLFQLISYSYILKAEKAGLVFPSKDKVIDNEIGNL
                     AGYGALLKKWSIQIPQKSSSYSAFCKMMENSEENFKAIIDEEVGRK"
     gene            complement(14554..>16069)
                     /locus_tag="VVE42_000926"
     CDS             complement(14554..>16069)
                     /locus_tag="VVE42_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HEU3073136.1"
                     /translation="PIFDMKRSTNEFIGRSAMVTKLLCIYTEGDPFFGVNINSQKEFW
                     NHFVSQTNQGGPYLQNHKIIELVSKTYPELEPSKLGTMLFEYSKLFMENKEDNSTMDS
                     SNNFSHQLTQSLLKSPNLILRGAPGTGKTYLAKEIAKELTDGNEDQIGFVQFHPSYDY
                     TDFVEGLRPVSNGDGAIEFRLQDGIFKDFCQKAKETQLIGGQDNFDEAWDSYLEYINV
                     AEEKEYITKTSYLSVNSRQNLSVNYDSGVPGWSLPSKYVYELYKDKNYNKQEYYKSGG
                     KTVLETLRKRFGLKDYISPEEVETDKKFVFIIDEINRGEISKIFGELFFSIDPGYRGE
                     KGSVSTQYANLHETDEKFYIPENVYIIGTMNDIDRSVDTFDFAMRRRFRFVEVTAEGQ
                     VGMLDKELNIHAEEAKIRLRNLNAAIENVQELNSHYHIGPSYFLKLKDVDFDYELLWS
                     DYIKPLLEDYLRGSYDEVETLETLKKAFELTNNEQKDQAVADDNEGDENDDADY"
BASE COUNT         4619 a         3572 c         2882 g         4996 t
ORIGIN      
        1 ataacaattt gccttttttt actttttctg atataatgag agaatattcg gaaaaggaga
       61 ctaaaaatga agaaaaaatt tctagcattt ttgctaattt tattcccaat tttctcatta
      121 ggtattgcca aagcagaaac gattaagatt gtttctgata ccgcctatgc accttttgag
      181 tttaaagatt cagatcaaac ttataaagga attgatgttg acattattaa caaagtcgct
      241 gagattaaag gctggaacat tcagatgtcc tatcctggat ttgacgcagc agtcaatgcg
      301 gttcaagctg ggcaagccga cgctatcatg gcagggatga caaagactaa agaacgtgaa
      361 aaagtcttca ccatgtctga tacttactat gatacaaaag ttgtcattgc tactacaaag
      421 tcacacaaaa ttagcaagta cgaccaatta actggcaaaa ccgttggtgt taaaaacgga
      481 actgccgctc aacgtttcct tgaaacaatc aaagataaat atggctttac tattaaaaca
      541 tttgacactg gtgatttaat gaacaacagc ttgagtgctg gtgccatcga tgccatgatg
      601 gatgacaaac ctgttatcga atatgccatt aaccaaggtc aagacctcca tattgaaatg
      661 gatggtgaag ctgtaggaag ttttgctttc ggtgtgaaaa aaggaagtaa atacgagcac
      721 ctggttactg aatttaacca agccttgtct gaaatgaaaa aagatggtag tcttgataaa
      781 attatcaaga aatggactgc ttcatcatct tcagcagtgc caactacaac tactctcgca
      841 ggattaaaag ctattcctgt taaggctaaa tatatcattg ccagcgattc ttcttttgcc
      901 ccttttgttt tccaaaattc aagcaaccaa tacactggta ttgatatgga attgattaag
      961 gcaatcgcta aagaccaagg ttttgaaatt gaaatcacca accctggttt tgatgctgct
     1021 atcagtgctg tccaagctgg tcaagccgat ggtatcatcg ctggtatgtc tgtcacagat
     1081 gctcgtaagg caacttttga cttctcagaa tcatactaca ctgctaatac cattcttggt
     1141 gtcaaagaat caagtaatat tgcttcttat gaagatctaa aaggaaagac agtcggtgtt
     1201 aaaaacggaa ctgcttctca aaccttccta acagaaaatc aaagcaaata cggctacaaa
     1261 atcaaaacct ttgctgatgg ttcttcaatg tatgacagtt taaacactgg tgccattgat
     1321 gccgttatgg atgatgaacc tgttctcaaa tattctatca gccaaggtca aaaattgaaa
     1381 actccaatct ctggaactcc aatcggtgaa acagcctttg ccgttaaaaa aggagcaaat
     1441 ccagaactga ttgaaatgtt caacaacgga cttgcaaacc ttaaagcaaa cggtgaattc
     1501 caaaagattc ttgacaaata cctagctagc gaatcttcaa ctgcttcaac aagtactgtt
     1561 gacgaaacaa cgctctgggg cttgcttcaa aacaactaca aacaactcct tagcggtctt
     1621 ggtatcactc ttgctctagc tcttatctca tttgctattg ccattgtcat cggaattatc
     1681 ttcggtatgt ttagcgttag cccatacaaa tctcttcgcg tcatctctga gattttcgtt
     1741 gacgttattc gtggtattcc attgatgatt cttgcagcct tcatcttctg gggaattcca
     1801 aacttcatcg agtctatcac aggccaacaa agcccaatta acgactttgt agctggaacc
     1861 attgccctct cactcaatgc ggctgcttat atcgctgaaa tcgttcgtgg tggtattcag
     1921 gccgttccag ttggccaaat ggaagccagc cgaagcttgg gtatctctta tggaaaaacc
     1981 atgcgtaaga ttatcttgcc acaagcaact aaattgatgt tgccaaactt tgtcaaccaa
     2041 ttcgttatcg ctcttaaaga tacaactatc gtatctgcta tcggtttggt tgaactcttc
     2101 caaactggta agattatcat tgctcgtaac taccaaagtt tcaagatgta tgcaatcctt
     2161 gctatcttct atcttgtaat tatcacactt ttgactagac tagcgaaacg cttagaaaag
     2221 aggattcgtt aatggcaaaa ttaaaaattg atgtaaatga tttacacaag cactatggaa
     2281 aaaatgaagt cctaaaagga attacgacta agttctatga aggagatgtt gtttgtatca
     2341 tcggtccttc aggttctggt aagtcaactt tcctccgtag cctcaatctt ttagaagaag
     2401 tcactagcgg tcacatcact gtgaacggct atgatttaac tgaaaaaaca accaatgttg
     2461 accacgtccg tgaaaatatc ggcatggtat tccaacactt caacctcttc cctcatatgt
     2521 ctgtattgga caacatcacc tttgctccta ttgagcacaa gttgatgact aaggaagaag
     2581 ctgagaaatt gggaatggag ttgcttgaaa aggttggact agcagataaa gctaatgcca
     2641 atccagatag cctatcaggt ggtcaaaaac aacgtgtggc catagctcgt ggcctagcaa
     2701 tgaatccaga catcatgctc ttcgatgaac caacttctgc ccttgaccct gagatggttg
     2761 gagacgtact taacgttatg aaggaattgg ctgagcaagg catgaccatg attatcgtaa
     2821 cccatgagat gggatttgct cgtcaggttg ccaaccgcgt tatctttact gcagatggcg
     2881 agttccttga agacggaaca cctgaccaaa tctttgataa cccacaacac cctcgtctga
     2941 aagagttctt agataaggtc ttaaacgtct aaactcaaac tgtaaggatt tccttgcagt
     3001 ttttctacct cgtattggaa tttttgattt ttcggaaaat tatgttagaa ttaagtttat
     3061 gaaatgaggt ttcctcatac ctagcaagac taggaataaa aatagaaatt aggtagctag
     3121 atgtcatcta aggttattgt tacaattttc ggtgcgagtg gagacctggc taaacgcaag
     3181 ctctaccctt ccctttttag actatatcaa tccggcaatc tttccaagca ctttgccgtt
     3241 attggaactg cccgtagacc ttggagtaag gaatattttg aatctgtagt tgtcgagtcc
     3301 atccttgatt tggcagatag taccgagcaa gcccaagaat ttgctagcca cttctactat
     3361 caaagccatg atgtcaatga ttcggaacat tatattgctt tgcgtcaatt acaagctgag
     3421 cttaatgaaa aataccaagc tgaacacaat aagctcttct tcttgtctat ggcacctcag
     3481 ttctttggaa ccattgccaa acacctcaaa tctgaaaaca ttgtcgatgg caaaggtttt
     3541 gagcgcttga tcgttgaaaa accatttggt acagattacg caactgcaaa caagttgaat
     3601 gacgaactcc tagcaacatt tgacgaagaa caaattttcc gtatcgacca ttatcttggt
     3661 aaggaaatga tccaaagcat ctttgcagtt cgctttgcaa acttgatttt tgaaaacgtt
     3721 tggaacaagg attttatcga caatgttcaa attacctttg cggagcgctt gggtgtagaa
     3781 gaacgtggtg gctactatga ccaatccggt gccctccgtg acatggtcca aaaccacact
     3841 ctacaacttc tttcgctcct cgccatggac aaaccagcaa gcttcacaaa agacgagatt
     3901 cgtgctgaaa agattaaggt ctttaaaaac ctctatcatc caactgatga agaactcaaa
     3961 gaacacttta tccgtgggca ataccgctct ggtaagattg atggcatgaa atacatctct
     4021 tatcgtagcg agccaaatgt gaatccagaa tcaacaactg aaacctttac atctggtgcc
     4081 ttctttgtag acagcgatcg attccgtggt gttcctttct ttttccgtac aggtaaacga
     4141 ctgactgaaa aaggaactca tgtcaacatc gtctttaaac aaatggattc tatctttgga
     4201 gaaccacttg ctccaaatat tttgaccatc tatattcaac caacagaagg cttctctctt
     4261 agcctaaatg ggaagcaagt aggagaagaa tttaacttgg ctcctaactc acttgattac
     4321 cgtacagatg cgactgcaac tggtgcttct ccagaaccat acgagaaatt gatttatgat
     4381 gtcctaaata acaactcaac taactttagc cactgggatg aagttggtgc atcatggaag
     4441 ttgattgacc gtattgaaga gctctgggct gaaaatggtg ccccacttca tgactataaa
     4501 gctggaagca tgggacctca agccagcttt gacctacttg aaaaattcgg tgccaaatgg
     4561 acttggcaac cagatatcgc ctatcgtcaa gatggtcgtt tagaataaaa aaatttcctg
     4621 caagtttatg ccttgcagga tttttgcttc tgattagatt aaaccttcca agagaccttt
     4681 catgaagttt tctgagttaa actctccaat atcatcgatt ttttcaccaa aaccaatcaa
     4741 ttttacagga atattgagtt cttcacgaat ggctagaacc acacctcctc gagcagttcc
     4801 atcaatctta gtcaaaacaa ttcccgttaa aggtgtgatt ttcgaaaatt ctttggcctg
     4861 tactagggca ttttgacctg ttgatgcatc aagtgccaag aaggtttcat gtggtgcttc
     4921 tggcacaaca cgtttgataa tacgaccaat cttttccaac tcagccataa ggttatcctt
     4981 attttgcaga cgaccagcag tatcaatcat gagaatatcg ataccttcag ccacggcacg
     5041 ttccatacca tcaaagacca cgctggctgg atcagctttt tcaggtccag ttactactgg
     5101 aacatctact cgtcggcccc attcagctag ctgagctact gcacccgcac ggaaggtatc
     5161 tgctgcaacc agcatgacct tcttaccagc tcgtttgtag cggtgggcta gttttccgat
     5221 agaagttgtt ttcccaacac cattcacacc aacaaagagc ataactgtca agttatcttg
     5281 gaagtggatg ctttcatcgt agctaccatc cttttcataa agctcaacca atttctcaat
     5341 gatgacacga cgaagtgcat caggtttctt ggcattttca agcttggctt cgtaacgtag
     5401 ttcctccgtt aagttagaag cgacttggac accaacatca ctcataatca acagttcttc
     5461 cagttcctcg aaaaattctt cgtcaacaga gcggaagtta gcaaagaagg cattcaagcg
     5521 ggcaccgaaa cctgtgcgag ttttcttaag actgcggtca tatttttcct gaacagtttc
     5581 ttctgtttga ggcctttctg gttcaagaat ttcagaatta ttttcttcta cagtctctag
     5641 aaactcagta ttcccttctt ctgagtgtgg aaattcttcg acttcttttt gagaaacttc
     5701 tacaactggc tctaaatcca gactttcttc aactgtgtct tggatttcct cttcttggaa
     5761 cacagcttgt tcaacaattt caacctctgc ttcttcctga gaaacttcct caacttctgt
     5821 gaaggtagga tcaacatctt cagacaaatc aagattttcc agagcttctt ttacaacttc
     5881 ttcgatttta ggttcttctt tttttccgaa tagacggtca aacaatccca tatcttagtt
     5941 ctcctttagc acatattctt cgatagccca ggcgacagct tcctcatcgt tggtcatcgg
     6001 cgtcactaca tttgcggctg cctttacttc aggaacagcg ttttgcatag caacaccaag
     6061 acctgcccat tcaatcatag agaggtcatt ggcctcgtca ccacaagcca tcacttgact
     6121 ttggtcgatt ccaagatggc tgattagttt tgccaaacct gttgctttat gaacattctt
     6181 tggtgaccat tctagcaaca tttcacgtga tttaaagatt tcatattggt caaacaattc
     6241 tggagaaatc ttctgaatgg ctgcatccaa gggttcttga gcaaaggcag tcacgcattt
     6301 gttgtaggtc atttgactag ataagtcttc aaagtccact ggaacaaagg tcaaagctgg
     6361 attgaatttg gcataaagac tttcttggtc cgattggatt tgataaactg ttccttctga
     6421 gatggcatca agaggcagtg ataatttctc tgtttcttca tacaaacgtg ccacatcatc
     6481 atatgaaaag actgttttat caaggatttc tcctgtattt ttctgaacta atccaccatt
     6541 aaaagtaatg gtatactcat cttcctgacc gtcagtccct aactcatgga gaaagaaatc
     6601 catggctttt aagggacgac cagttgtcaa tacgaccttg ataccacgat cacgcgcagc
     6661 tttcaaggtt tccttggtac gatccgtcag ccttttatca gtagtcagca aggtcccgtc
     6721 caagtccaat gcgattaatt ttatatctgc cattataagc cctccataca agctataacc
     6781 gactggtcct tatggtgacc aatcacagtc tttgctaatt ctaaaatttc aggtcgtgca
     6841 ttttcaggag ctacaggatg tcccacaacc tgcatcatat gtaagtcatt aagattgtct
     6901 ccaaaagcca tgacctgatc cattgtgata ccaagttttt tagctaattc aacaatagct
     6961 acacccttat cgacatagtc cagaacaata tcaatagatt caaagccagt tgtcatagcc
     7021 ttaacaccag gaacgttttc gtttacccaa gcctccccat cttccagcgt ttcttctgtg
     7081 aagttggttg taaatttgaa aatgtcatct gtgatatctt ccaaactcgc tactttttgg
     7141 atattttcat tataatgctg actcactttc aaataggtct catcaaccgt atctagaaca
     7201 tatgaaccct tcttacccgt caagagcagt ttattgatat ctacataagg tgacgttttc
     7261 agcttttcaa aagttgccag ataaaagtca cgagacatag tcgcttcata caagtcctga
     7321 ccttgatact ctaccaaact gccattttcc gcgatgaaaa taatgtcatc acgaacatca
     7381 gcaaataatt tttccagaga cagaaatccc cgacccgaag ccaccgcaaa gtaaatccct
     7441 ttttccttgt aggaagccaa gagagacttg agacgatcca tatcaaagcg tccattccca
     7501 tctaggaagg ttccgtccat atccgttgct actagtttaa ttgtcatcct tcaatacttt
     7561 ctaaatcttt taacttaact gaaacaatct ttgaaacacc cgattcttgc atggtcactc
     7621 catagatgga atcagccgct gccatggttc ccttacggtg ggttacaacg ataaactggc
     7681 tgtccttgtc aaagcggttg aggtaatccc caaaacgttt aacattggct tcatccagcg
     7741 cagcttccac ctcatccaag atgacaaaag gaatggtctt gacacgaata atggagaaaa
     7801 gcaaggcaag agccgataga gctttttcac caccactcat gaggttaagc gactggattt
     7861 tcttacctgg aggttgaaca gaaatctcca caccagctgt taaaaggtcg ccctcagtca
     7921 atatcaagtc tgcctgacct ccgccaaaca tctgcttgaa ggtcacttta aaggactcac
     7981 gaatagcttc aaaggttgat ttaaagcgtt ccttgacctc atcattcatc tctgtaatgg
     8041 tttcaaggag cagatttttc gctgacaaaa tatcatctcg ctgactattt agaaagtcca
     8101 gacggttgtg aacttcttcg tactggtcaa tagcttccaa gttgacaggt cccaatgagc
     8161 gaatagcctt ctctaaatcc tgcacctctt gctctgccag attgaggttt tccaattcat
     8221 gtgccttttc tagtgcttca gtatagctaa tctggtactg gtctgttaat tgattttgta
     8281 gatggcgcaa gcgctcgctg accttttctt tcttggcttc agcacgtgtt tgcttgcgaa
     8341 tccactcctc attctgctgg cgagcttgat ccaagtgact ggcaatatca tccagttgac
     8401 cttctatatc atccaactca aactgcttgc gaatcaaacc ttgttggaga tttgttttct
     8461 gagttttgga ttcttccgcc tgttgactga gcaattctgt atcaaccttc tcaagattgt
     8521 caactttttc ttggagcatg cgctgaattt cttcttgttc gatattaaga ttgtccaatt
     8581 ccttgcctaa gcgttcaata tcagccactt cataacgttt ttgcccttgc agttctgtct
     8641 taagcaaacg agcttgcgct agctcttcct gcaagttttg atagcgttct tggatggcat
     8701 ttttgttaga cttaatctct tcaatctcag cttccagatt ttgcttgtca ctggcgattg
     8761 cagcaagacg ctcttggcag ttttccttgt ctgcttgcca atctccatca gaaagacgat
     8821 ctatttcctc ttcttggagt ttccaaagag tttctagctc ttcaacttgc tgactggtct
     8881 gctgataagc gagggacaag ccttgctcct gaatacgtgc ctgttctcct tgagatttga
     8941 tggcttctaa taattcggac aatctagcca tctggtcttg taaggtcttc aaagtcgcct
     9001 cttctgaacc caagcttgct tcttcttcag caatttcttt ttgtaattgc tccagttctg
     9061 gcttgatgaa aatactgtta ttctgacgat tggctccacc tgcgtaagaa ccacctgtgc
     9121 gcaactctgt accgtccaaa gtcaccatac gaacctgata acgaactttg cgcgctgctg
     9181 cacgcgcatg ctcaacggta tcaaaaatcg ctgtcgtagc tagcaagttc ttgaaaatag
     9241 cttccagtct cgtatcgaat gtcaccaact catctgccat gcctagaaat cccgcacttg
     9301 cagcgataac atcttggttc tgactagaaa tcgtacgcgc cttgatagtc gtcaacggaa
     9361 ggaaggttgc acgaccagct ctatttcgtt tgaggaaatc aatcgccttg gttgccgact
     9421 cttcatcttc tacgatgata tgctgactgc tggctccaag tgcaatctct aaggcagttt
     9481 gataatgcac atcaaaggtc aaatgctcac taacagcacc aataatccca ccaaggcggt
     9541 ctttttcttg gagaacactc tttacacctg cataaaagtt actgtgattt ctaaggatgt
     9601 tttccaaact ttgagctcta gcctgcttgt ttttaagact atccagacgg tcaaagagtt
     9661 gactttgttg agcttgatag gaagtttttt gctcctcttg ctccttggca atagcttgat
     9721 agtcagccaa taatttctga acctgcacct tggcagtttc aagctcgtct ttttgctgac
     9781 tagccttctc tttagctgta gctaattgct ctttcagctt ttctagttga tctgcttgtt
     9841 tttgagaaag ctgacgacta ttttccaact cattctcaat acgggtcaac tggtttgaga
     9901 catccgcttc ttcttgtaaa agagctacaa agcgttcacg taagagctca atcatctgat
     9961 caggatcgtc tgagaaagcc agcaattcag cttctaaacg attgagtttt tgattatttt
    10021 ggactagatt tccctctaac agagctaaag agctttcttt atcatacttt tctttgctga
    10081 gtgaatttct cttatcctcc aaagcagcca aacgagcttg tgcctcctgt tgattcaggg
    10141 ccacttgctc ggactccagt ttcgataggg ctaattttct ttctaaatca ctaatcagac
    10201 tagtcaagtc catcaaactg ccttggtctt tggccatttc agcctgtaaa tcttggcgtt
    10261 gctttttaag agtttgattt tcttcttcta atttttcacg cttttggtaa taactcatca
    10321 agagttcttg aacctgagcc aactcttctt ctgtcgactc tagttctgcc ttattttcct
    10381 tgatttgagc aaccagaacg tctaaataaa tagccttacg ttgtccttcc aagtctaaaa
    10441 acttacgggc attctcagct tgcttctcaa gaggcttgat ttgattatcc aactcgtaga
    10501 taatgtcctc taagcggtcc agattatcct gagtttgctg cagtttactc tcggtttctt
    10561 ttctgcgagt cttgtatttt aaaactccag cagcttcttc aaaaatagct cgtcgttcct
    10621 caggcttgga attaaaaatc tcctcaacct tcccttggga aataatagag aaggaatctc
    10681 gtcccaatcc agtatccaag aagaggtcat gaatatcacg cagacggact ttcttgccgt
    10741 caatcttgta ttcgctatct ccactacgat agatatggcg ttccaccctg atttcttgac
    10801 ctgcatcctt gataaatccg tcatgattat ccagagtcac aactacagaa gcataattga
    10861 gcggtttgcg actttctgtt ccagcaaaga tgacatccgg catcttgccc ccacggagac
    10921 tcttgacact agactccccc aaagcccaac gcagactttc tgtaatattg gactttccag
    10981 atccattggg tccaacaact gccgtcacac cttggtcaaa aacgaccttg gtcttatcag
    11041 caaaagactt gaacccctga atttcgattt cctttaaata catgaatcca gccctttctc
    11101 aacggcattt ttggcagctt cctgctctgc taatttctta gaacgacctt ggccttgacc
    11161 gatgctctta ccttcaacaa gaacttctac atcaaagacc ttatcgtgag caggccctgt
    11221 ttcagaaatc acctgataac gaatagccac atcaccattg acctgaagca actcttggag
    11281 atgggtttta tagtctgtaa tcatctcaaa ttcgcctgct tctaccttag gaatcataac
    11341 ttggtagata aattccttga cctttgccac atccttatcc aaaagaaggg caccaagaaa
    11401 ggcttcaaag gcatcaccaa gaatggtgtc acgattgcga ccaccagatt tttcttcccc
    11461 tttacccaac ttgataaact ggtcaaactg gcaatcacgc gcaaaaccag ctaaactctc
    11521 ctcacggaca atcatagcac ggagttttga taggtcacct tcaggctttt taggatattt
    11581 tttatataga tattctgaaa tcaataactg tagaacagcg tctcctaaaa attccaagcg
    11641 ttcattgtgt gaaattttta agaggcggtg ctcattggca taactcgtat gagtaaaggc
    11701 agtttccagt aactttttgt ctgcaaattc gatttcaaaa tgattcttta gtacagtttg
    11761 taattctttc ataccaactt ctttctaact gataatagtc ctttttatta tatcaaaaaa
    11821 agcctcctga gtcactctaa aacgggactg gaaagcattt gggaattctt tagacagaga
    11881 ttctcagttt tagcggcaaa tttgggtcag gataaagaaa aaagccctat taaaggcttt
    11941 ttaggatgtt tacatccacc ctgagggaat cgaaccccca tctcaagaac cggaatctta
    12001 cgtgatatcc attacactaa gggtggaaac ttgttttatt ataacagaaa tttgctctaa
    12061 taacaagttt tttggtcaaa taccccgtct tagtgggaag catccccatt ccagatggag
    12121 tttttcacga tcacataatc aacgtgttta aggtcagcaa cctgacgtcc acctgcataa
    12181 gaaatagcac tttgaaggtc ttgttccatc tcagttaaag tgtcttgcag atgaccttta
    12241 gcaggaagca agatacgttt gccttccaca tttttgtaag cacctttctg atattgtgag
    12301 gctgaaccat aatattcttt gaactgttca ccatcgactt caatcgtttt ccctggactt
    12361 tcaatgtgtc ctgcaaagag ggaaccaatc atgatcatgc tagcaccgaa gcggatagac
    12421 ttggcaatat caccgtgagt acgaattcct ccatcagcga taatcggttt acgcgcagcc
    12481 ttggcacacc agcgtagagc agccaactgc caaccacctg taccaaaacc agtcttaacc
    12541 ttggtgatac aaaccttacc aggaccgatt ccgaccttag tagcatctgc accagcattt
    12601 tccaattcac gcacagcttc tggtgttccc acatttccag caatgacaaa ggtatctggc
    12661 aattctttct tgatgtgttg aatcatagaa atcacgctat ccgcatgacc atgagcaata
    12721 tcaatagtga tatactcagg agcatcagcc ttgagctggc taacaaaatc atactcataa
    12781 tccttaacac cgacagatat ggaagcaatg agcccttgat cgtgcattcg tttaataaaa
    12841 ggaatgcgtc ctgcctcatc aaaacggtgc ataatgtaga agtaaccacc tttagccagt
    12901 tgctctgcta cattttcatc caaaatcgtc tgcatattcg ctggcacaac aggtagttta
    12961 aaggtgtgat ttcctaaagt gacacttgta tccgcttctg cacggctttt aatgacacat
    13021 ttatttggaa tcaattgaat atcttcgtaa tcaaaaattg gaaattcatt taacatatcg
    13081 atgtctcgtt tcttttgtaa tgacctacct atgctctcgc atcactacgc cttttccgac
    13141 gtttccttaa tttattataa accaaagtac agtttttgtc aaattatttt atgaattaaa
    13201 atatattgtt cggattttgc ttgtgttaaa aataaaagaa agaggggaaa ttattttctc
    13261 cccacttctt catcaataat cgctttaaaa ttttcttctg aattttccat cattttacaa
    13321 aatgcactat aagatgaaga cttctgagga atttggatag accatttctt caacaaagct
    13381 ccataacctg ctaggtttcc tatctcatta tcaatgacct tgtccttgct cggaaaaaca
    13441 agtccagcct tctcagcttt taaaatataa gaataggaaa tcagctggaa taagtcctca
    13501 cggttgattc ctttttcagt caattccagt tttttatatt ttgcatctag aacaatcttt
    13561 cgttctctgt cataaaaatc tggatatact tttcgtttcc caacagaaaa tactgaaatt
    13621 ccatccgtct tatccttatt tctgggatgt acaaaacctt ttggcaacaa ggtgtaaaca
    13681 tactcttccc aaagccaggc aacatcaaag agaataccat agattttttg atcttgatac
    13741 cctaaaccgt gcttttcttg gtttaggatc atcagacaaa gttcttgtaa gtttctgtac
    13801 tcgtgaaagt atgcatgacg tataggttta gattgatttc tccgagtaat cttagcacga
    13861 tcagctagtt tataagaggg cgttacacgc acgatttcag atacgttttc acgactagtt
    13921 gagagattat ctagtacccc ttgaccaatg cttttctgat tcttaatgta ttcaatagtg
    13981 tgacggacca actgcatgag gggattatca taggtgaact ctctcgttgt gtaggcaata
    14041 tttcccgtga aaggaagatt tttcttgaga tggtttctta caacaatcac tcccttaaca
    14101 ttactgtcgt tatgagaaaa tcgatgatat tccttataaa gaccttttcg aatagcagct
    14161 tgtagatact tggggaagag atacatcaaa agttgataaa gcctctcttc acgagacaaa
    14221 gcaacatcta aactagtgag attgatataa agaaccttgt ttaagagata atgcaaaaaa
    14281 tggtcattac tctcatcaga aaatcgtgag gaaatcgtta atctttcctg accatatcca
    14341 agaaaaccaa tcacgttccc tgtcttgatt ttctgattga ctgtttcaaa aatcttttga
    14401 tccttatcca aatcaggagt atgagtcaaa tcatttggaa aaatgaaaat acgttcatct
    14461 tgggatagac tttccaaggt tctgtctaaa agaagattgc ttagtttagg atattcttca
    14521 acaaattttt ctttaataat cttgtgttga ttatcagtaa tccgcatcat cgttttcatc
    14581 accttcattg tcatcagcta ctgcctgatc tttttgctca ttatttgtca gttcaaatgc
    14641 ttttttcaaa gtttccaaag tttcaacctc atcataagaa cctcgcaagt agtcttctag
    14701 gagaggctta atataatcag accagagtaa ttcatagtca aaatctacat ccttcaactt
    14761 aagaaaataa cttggtccaa tatgataatg actgtttaat tcctgaacat tttcgatagc
    14821 agcgttcaag tttcttagac gaatttttgc ttcttctgca tggatattca gctctttatc
    14881 caacatgcca acttgaccct cggcagtaac ttcaacaaaa cgaaaacgac gacgcatagc
    14941 aaaatcaaag gtatccactg aacgatcaat atcattcata gttccgatga tgtaaacatt
    15001 ttcggggata tagaactttt catcagtttc gtgtagattt gcatattggg tagaaacact
    15061 tcctttttca ccacgatagc cagggtcgat agaaaaaaag agttcgccaa aaatcttaga
    15121 aatctcccca cgattgatct catcgatgat gaaaacaaat ttcttgtctg tttcaacctc
    15181 ttctggagaa atatagtctt tcaaaccaaa tctctttctc aatgtttcta ggacagtttt
    15241 tccaccactt ttgtagtatt cttgcttatt ataattttta tctttataca actcgtaaac
    15301 atatttgcta ggtagtgacc atcctggaac accactatca taatttactg acaaattttg
    15361 tctactatta acagataagt aagatgtttt tgttatatat tctttttctt cagcaacatt
    15421 tatatattct aagtaagaat cccaagcctc atcaaaatta tcttgtcctc caatcaattg
    15481 ggtttctttt gctttctgac aaaaatcttt aaaaataccg tcctgtagcc taaactcaat
    15541 agctccatcc ccatttgata ctggtcttaa accttctaca aaatccgtat aatcatagga
    15601 tgggtgaaat tgtacaaatc cgatttggtc ttcgttacca tccgttaatt ctttggcaat
    15661 ttctttagca agataagtct ttcctgtgcc aggagcacca cgaaggataa ggtttggaga
    15721 ctttaataga gattgagtta attgatgact aaaattgttt gatgaatcca ttgtactatt
    15781 gtcttcctta ttttccataa aaagcttaga atactcaaaa agcatagttc ctaatttcga
    15841 tggctccaac tcaggataag ttttggacac cagttcaatt attttatgat tttgcagata
    15901 aggtccacct tgatttgtct gagaaacaaa gtggttccaa aattctttct gactattaat
    15961 atttacacca aagaaaggat ctccctcagt atagatacaa agtaattttg tcaccatagc
    16021 agaacgacca ataaattcat ttgttgatct tttcatatca aaaatagga
//
LOCUS       DAVGJL010000035        15970 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.035, whole genome shotgun sequence.
ACCESSION   DAVGJL010000035 DAVGJL010000000
VERSION     DAVGJL010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15970
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.035"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..632
                     /locus_tag="VVE42_000927"
     CDS             <1..632
                     /locus_tag="VVE42_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEU3073137.1"
                     /translation="TLFHLNQILEANNSSFIIEDGDLEIMKRAAALNTMFGMNYYRSE
                     FIREYKGENRQEFNSTGIKGQSSFKLNALGEFVKKPGIPTTDWDWNIYPQGLFDMLLR
                     IKEEYPQHPVIYLTENGTALKEVKPEGENDIIDDSKRIRYIEQHLHKVLEARDRGVNI
                     QGYFIWSLQDQFSWANGYNKRYGLFFVDYETQKRYIKKSALWVKGLKRN"
     gene            701..2380
                     /locus_tag="VVE42_000928"
     CDS             701..2380
                     /locus_tag="VVE42_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEU3073138.1"
                     /translation="MDKLVAAIEKQQGKFEKISTNNYMMAIKDGFIATMPLIMFSSFL
                     MIIIMIPKNFGVELPSPAIVWMRKVYMLTMGVLGVIVSGTVAKSLAGNVNRKMPPGKV
                     INDISVMLAAICSYLVLTVTLVVDEKTGSTSLSTNYLGSQGLVTSFVSAFITVNVYRF
                     CIKRDITIHLPKEVPGAISQAFRDIFPFSFVLLISGLLDIVSRFSLDVPFAQVFQQLL
                     TPIFKGVESYPAMMLIWFMCALLWFVGIHGPSIVLPAVTALQLSNMEENAQLLANGQF
                     PYHSLTPNFGNYIAAIGGTGATFVVPFILIFFMRSKQLKSVGKATITPVLFAVNEPLL
                     FGMPVILNPYLFVPFLMTPPVNVFLGKVFIDFFGMNGFYIQLPWAFPGPLGLLIGTNF
                     QSISFVFLSLMLVVDILIYLPFCRAYDRQLLMKEDVVSSNDIILEEETSEIMPGEIDV
                     IKSKELKVLVLCAGSGTSAQLANAINEGAQLAEVRVIANSGAYGAHYDIMGVYDLIIL
                     APQVRSYYREMKVDAERLGIQIVAARGMEYIHLTKSPSKALQFVLEHYQAV"
     gene            complement(2506..3945)
                     /locus_tag="VVE42_000929"
     CDS             complement(2506..3945)
                     /locus_tag="VVE42_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEU3073139.1"
                     /translation="MNKSMIRYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSLIDAF
                     FEISSGFTTTGASILNDVSVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLLYLALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRIKAFFGDEELRAYL
                     VIVLVSTGLISLNTLYLYPGFSKSFEMTFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMAIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNKTVIDKDTQHK
                     ILKYFVIYAMILLALIFIVSLDSNDFLVVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            complement(3949..5298)
                     /gene="trkA"
                     /locus_tag="VVE42_000930"
     CDS             complement(3949..5298)
                     /gene="trkA"
                     /locus_tag="VVE42_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEU3073140.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVSRFD
                     IIGILGNGADFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIERDHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKGVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            5486..6334
                     /locus_tag="VVE42_000931"
     CDS             5486..6334
                     /locus_tag="VVE42_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HEU3073141.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     EQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVYQNQVVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            6349..6897
                     /locus_tag="VVE42_000932"
     CDS             6349..6897
                     /locus_tag="VVE42_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HEU3073142.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLVGYGLWWTWIIASGLFGLVVGLFRKYVRVING
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKKD"
     gene            6904..7161
                     /locus_tag="VVE42_000933"
     CDS             6904..7161
                     /locus_tag="VVE42_000933"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073143.1"
                     /translation="MKIKEQTRKLAAGCSKQCFEIVDRTDEVSSKHGFEVVDETDEVS
                     NHTYGKAKLTWFEEIFEEYKMMGKAGQLVFFDVYRLVRQVS"
     gene            7263..8945
                     /locus_tag="VVE42_000934"
     CDS             7263..8945
                     /locus_tag="VVE42_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3073144.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQAAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLLPLLDQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHRPVDRIV
                     LINDGRILFNGSPDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKEEQLANLDNLSIS
                     KGQVQLQNELAKETPALQSLFRLEEVSFSYDDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKAISSFIQTEGRYLWEKQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVDEKEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHQKGHTIVMITHDMQLMLDYSDRVLVMVDGELIA
                     DTVPASLLSDPELLVKANLKETSIFNLAKKLDVDPLDLTAFYKERREGCKLN"
     gene            8930..9760
                     /locus_tag="VVE42_000935"
     CDS             8930..9760
                     /locus_tag="VVE42_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEU3073145.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLAAMISYDTRMLL
                     LIAIFSVFLLYLSEIRFKDVSFVAVFATVFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGVYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQALKKGDYITLLLATLFLVVSLLLILQNQGRFYNPWK
                     "
     gene            9953..10456
                     /locus_tag="VVE42_000936"
     CDS             9953..10456
                     /locus_tag="VVE42_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEU3073146.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFMSQMKGKLYLISKFAEKIYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     regulatory      10510..10744
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            10909..11472
                     /locus_tag="VVE42_000937"
     CDS             10909..11472
                     /locus_tag="VVE42_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU3073147.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGLVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTVGLTLAMLVLNYVYAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYVLLKPTLKHYER"
     gene            11486..12112
                     /locus_tag="VVE42_000938"
     CDS             11486..12112
                     /locus_tag="VVE42_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU3073148.1"
                     /translation="MKGSFALLLFVILGYMVKFYPEMLVNFDQSIQTAIRGDLPDYLT
                     ILFRALTRLIDIPVIITWVVITAFVFYRKRWKIESFFMLGNLALAGLLIVTFKNIYQR
                     PRPAILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKDQVWRKIVQIVLGLYLVS
                     VLVSRVYLGVHYPSDVLASLCVGLGVLFIEFPFYDKLRFQWRFKGKQK"
     gene            12140..12529
                     /locus_tag="VVE42_000939"
     CDS             12140..12529
                     /locus_tag="VVE42_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HEU3073149.1"
                     /translation="MKVNIADLHLTQLYLSEKKLQDIQMLYQSAETIQVDPISILAFG
                     DCLLITDGHHRAYQALLAGRDTISAEWDRDGGDELYHLYAQACEERKIYSVFDLEDRI
                     LAQDGYEAKWYNWCDGFNQAATLLLKR"
     gene            12534..12983
                     /locus_tag="VVE42_000940"
     CDS             12534..12983
                     /locus_tag="VVE42_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEU3073150.1"
                     /translation="MCFVPYYKVNHCEEAFAWYQDVNLVYLVDGVKLPYSQATLEAMY
                     SYLDRHGELFWIEVKEKGEWFPIGDITLSQDNLPIVIGNSAYQHRGLGKKILSALIEL
                     ARVKGWKELRVKKIYTYNHASRRCFKSLGFVENGATEKGMSFILELV"
     gene            13111..13698
                     /locus_tag="VVE42_000941"
     CDS             13111..13698
                     /locus_tag="VVE42_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEU3073151.1"
                     /translation="MKLEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
     gene            14088..15695
                     /locus_tag="VVE42_000942"
     CDS             14088..15695
                     /locus_tag="VVE42_000942"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEU3073152.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKVAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPAADMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYVNDAEVKINWVNAND
                     VTAENVAELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDIEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENSN"
BASE COUNT         4679 a         2856 c         3603 g         4832 t
ORIGIN      
        1 acacgctttt tcacttgaat caaatattgg aagctaataa ttctagcttt attattgaag
       61 atggtgattt agaaattatg aagagagctg cagctcttaa tacgatgttt gggatgaatt
      121 attatcgttc agaatttatt cgtgaataca aaggtgaaaa tagacaagaa tttaattcaa
      181 caggaataaa aggacagtct tcttttaaat taaatgctct aggtgaattt gtaaaaaaac
      241 ctggtattcc gacaacagat tgggattgga atatttatcc tcaagggtta tttgatatgt
      301 tgcttcgtat caaagaagaa tatcctcaac atccggtcat ttatttaact gaaaatggta
      361 cagcccttaa agaagttaag ccagagggcg agaatgatat tattgatgac agtaagagaa
      421 tccgttatat tgagcaacat ttacacaaag ttttagaggc tcgagatagg ggagtcaata
      481 ttcaaggcta ttttatatgg tctttgcaag atcaattttc ttgggcgaat ggctacaata
      541 agcgatatgg tcttttcttt gttgattatg aaacacagaa gagatatatt aagaaaagtg
      601 ctctttgggt aaaagggcta aaacggaatt aaggttagcg atttgaatga tgtttaatat
      661 gttttaaata tgaggttgaa ttttttataa gaggagtttt atggataagc tagtcgctgc
      721 cattgaaaag caacaaggga aatttgaaaa aatttctact aataactata tgatggctat
      781 taaagatgga ttcattgcta ctatgccttt aattatgttt tcaagctttt tgatgattat
      841 tattatgatt cctaaaaatt tcggagtaga gttaccgagt ccagctattg tctggatgag
      901 aaaagtgtat atgttaacca tgggagtttt gggtgttatt gtttcaggga cagttgcaaa
      961 gtcattggct ggaaatgtta atagaaaaat gcctcctgga aaggtaataa atgatatttc
     1021 tgtaatgttg gcagccatat gtagttatct ggtattaact gtaacgcttg tagttgatga
     1081 gaagacggga tctacaagtt tgtcgacaaa ctatttagga tctcaaggat tggtaacttc
     1141 gtttgtcagt gcctttatta ctgtaaatgt ttaccgattc tgtattaagc gagacattac
     1201 tattcattta cctaaggaag ttcctggggc tatatcacaa gcttttagag atatttttcc
     1261 tttttctttt gttttactta ttagtggttt gttagatatt gtatctcggt ttagtttaga
     1321 tgttcctttt gcccaagtat ttcaacaact attgactcct atttttaagg gggtagaatc
     1381 atatcctgct atgatgttga tttggtttat gtgtgctttg ctttggtttg ttggaattca
     1441 tggaccatct attgtcttac ctgctgttac agctttacaa ctgagcaata tggaagagaa
     1501 tgctcaactt cttgcaaatg ggcagttccc ttatcattct ttaacaccta atttcgggaa
     1561 ttatatcgct gctattggag gaacgggggc tacctttgtt gtaccattta ttttgatttt
     1621 ctttatgcgg tctaaacaat taaaatcggt aggtaaagct acaattactc ctgttttatt
     1681 tgcggtaaat gaacctcttc tatttggtat gcctgttatt ttgaatccct atctttttgt
     1741 cccttttttg atgactccac cagtaaatgt atttctagga aaggtcttta ttgatttctt
     1801 tggaatgaat ggattttata tccagttacc ttgggccttt cctggtccct tgggattgtt
     1861 aattggaacg aattttcaat ctatctcctt tgtattttta tctttgatgt tagttgtcga
     1921 catattgatt tatttgccat tctgtagagc gtatgataga cagttactga tgaaagaaga
     1981 tgttgtaagc tcaaatgata ttattttaga ggaggagaca agtgaaataa tgcctggtga
     2041 gatagatgta ataaaaagta aggagttgaa agtactggtt ctttgtgcag ggtctggaac
     2101 aagtgcgcaa ttagccaatg caattaacga gggcgctcaa ttagcagaag ttagagtgat
     2161 tgcgaattca ggagcgtacg gagctcatta tgatattatg ggtgtttatg atttaattat
     2221 tctggctcca caagttcgga gttattatag agagatgaag gtggatgcag aaagattagg
     2281 tattcagata gttgctgcca gaggaatgga atatattcat ttaacaaaga gtccaagtaa
     2341 agccttacaa tttgtattgg agcattacca agctgtgtag taagtttttc catcttttat
     2401 ttgagtaaag attttgctta cagataggct tggatttaaa aacgttcccc cttttttaat
     2461 ataagaatcc ctctttcaca attgtaaaaa gagggatttt gtattttatc tcttagacca
     2521 agttctcttc ataaagagaa ggaggattgg gtaaatctcc aagcgccctg caatcattgc
     2581 aaaggatagg agaatttttg agatgggact aaagattgag aaactagaag tggttcctag
     2641 aataggcccg atattattga aacagctaaa gacagcgctg gtcacgacca gaaaatcatt
     2701 gctatctagg ctgacaataa agataagcgc tagcaaaatc atagcataga tgacaaagta
     2761 cttgagaatc ttatgctggg tatctttgtc aatcaccgtt ttattaacat ggagggtcaa
     2821 aacacggtgg ggcgatagga ttgacaaaat ttggtttttg gcaatttttg aaaggatgag
     2881 gcctcgaata atcttgagtc cacctgcagt tgatccagca gagccaccga ttgccatgag
     2941 gaaaaggagg ataaactggg agaagagggg ccagttggta atatctccat atccaaaacc
     3001 agttgttgta atgatgttgg aaacctggaa gaaggtcatt tcaaagctct ttgaaaaccc
     3061 tgggtagagg tagagggtgt tgaggctaat caagcctgta gaaaccagta caatgaccaa
     3121 gtaagcccga agttcttcgt caccaaagaa ggccttgatg cgacggagca tgaggtagta
     3181 gtagaggttg aaatttaccc caaaaaccag aactccgata ctgaccagat aggtaatcag
     3241 tgagctgcca tagtgggcaa ttccgtcgtt atagacggta aagcctccag ttcccgctgt
     3301 ccccatagca atgacaaaac tatcgtagag aggcatacca gctagataat agatgatgac
     3361 aaagagggag aagagagcta gataaaggag atagagaatc tgggcagtgt tttttagttt
     3421 ggatacaacc ttgccaaaaa caggacctgg aacctcagcc ttcatcacct ctaggtggct
     3481 atttttggca ttgtccataa tagcaagtgc aaaaacaagc actcccatcc ctccaatcaa
     3541 gtgggtaaaa cttcgccaga agaggaggga acggctgaga accgaaacgt cgttcaaaat
     3601 acttgctcca gtagttgtaa acccagaact aatttcaaaa aaggcatcaa taaggctggg
     3661 gatttgccca gaaaagacaa aggggagacc accaaagaaa gaccaaagga tccaacagag
     3721 ggcaacgatc aagactccct ccttggcata aatccgttga ttttttggct tctgtaaact
     3781 ccctgaaccg cctaacaata cgagaatccc tatggtcgaa aagagggctg taaagacttg
     3841 gctcgattca cggtaataga cagcgatagc cacaggaacc aagaggagaa cagcttcaat
     3901 caaaagtaac tttgaaagga ggtaacgaat catactttta ttcatttctt acctcgcaat
     3961 caagtcataa atcttggtga tgtttggcaa caaggttgtt actaggagct tgtctccaac
     4021 ttccaacata tcctccccag ttgggaaaat agtcttgccc tttcgaataa tggctgcaat
     4081 aagaacccct tttttcaatt tcagttgaga aagaggtttg gcagtcattt tattggcttc
     4141 cttgatatgg aattgcaggg tttcgatttg gccattggct agatggtgca tggcttgaag
     4201 gtctgaatac tgggcattaa ctcgaccacg aataaagtgc ataatcgtat ctacagcgat
     4261 gcttttaggt gtgatgatac ttgaaaaatc aggcgcattg ataatctcga ggagactggt
     4321 acgattgacc ttagtaatat ttttctgtac acctaccctg tcaaggaaca tagatgtaat
     4381 cagattttcc tcatcgactc ctgttagagt cgcaacggca tcatagtgtt gagcactttc
     4441 ttccagcagg atatcttttg cggttccatc tccttgaacg atgtagagat ttgggaattt
     4501 ctcgctaaag aagctggcga tttcaggatt gatttcaatg acttttgtat cgatacgact
     4561 atctttgaga ataccaagta gataataggc aattctacct gctccaacga tgagaaggct
     4621 cttcacggcg cgtgatttaa aataattatg gaagagtatc atatcgacac ggttaccagt
     4681 gacaaagatt ctatctttat cctgtacagt catgtcaccg cttggaatga taatttgatg
     4741 atccctctct atcgcacaga caatgacatt accaaatttt ttacgaaaat cagaaatggg
     4801 catttggcaa agaccgctgg tggacttgac gacaaattcc atgaggctaa cgcgtccacc
     4861 agcaaagcgt tcgacagaca gggcgttggg gaagtcaatg atattcgcga tagcgcgggc
     4921 agccaagagc tcaggattaa cgataagaga aaaaccgaga atattctttt ccttgaaata
     4981 agagttagaa tattcagggt tccgcacccg aacgatagtt tctttagctc ccattttctt
     5041 ggctagaact gctgcaatca tgttgacttc atcgtgctca gtcagggcga taaagatatc
     5101 acaatcttgg acgctggctt gctcaagaat ggcaaaatcg gccccgttac caaggatacc
     5161 aatgatatca aagcgactga caatatgatt gagaacagct tcgtcttgct caatcagcaa
     5221 aacatcatgc ttttctgcaa ccaaggagcg acagagggca aaaccaactt ttccccctcc
     5281 gacaaggata attttcataa taaaacctac tttttcatga tgtaactatc ataccctttt
     5341 tcaagaaaaa atgcacctac tagctaataa caagagcttt tagtgaaaat tcgctataag
     5401 gtaaaactat accctaacca attgaaatag ctattagcga ctttctctga aatatggtat
     5461 gataaaggat atacaaggag ataaaatgaa taataattta ctggtattac aatcagactt
     5521 tggtctggtt gatggtgcgg tatcggctat gattggagtg gctttagaag agtctccaac
     5581 cttaaaaata catcacttga cgcacgatat cacgccttat aatatttttg aggggagcta
     5641 tcgtctcttt cagacggtgg attactggcc tgagggaacg acgtttgtat cggttgtcga
     5701 tccaggtgtc ggttcgaaac gtaagagtgt agttgccaag actgcaaaaa atcaatacat
     5761 tgtcacgcca gataatggga cgctttcctt tatcaagaaa cacgttggca ttgtagccat
     5821 tcgtgagatt tctgaggtgg ccaataggcg tcaaaacaca gagcattctt ataccttcca
     5881 cggtcgtgat gtctatgcct atactggtgc taaactggcc agtggtcaca ttacttttga
     5941 ggaagtaggg ccagagctca gtgtggaaca gattgtagag cttccagtcg tagcgaccat
     6001 catagaagat catctggtga agggagccat tgatattctg gatgtgcgtt tcggttcgct
     6061 ttggacctct atcacacggg aagaatttta caagctggaa ccagaatttg gtgatcgttt
     6121 tgaagtgacc atctatcatg ctgatatgct ggtctatcaa aatcaggttg tctatggcaa
     6181 atcatttgca gatgtgagaa ttgggcaacc catcctttac atcaactctc tctatcgttt
     6241 aggtttggct atcaaccaag gttcctttgc caaggcctat aatgtaggtg tcggttcatc
     6301 ttggaccatt gaaataaaga aaatagaagg ataaaatagg agaatagtat ggaaatcaaa
     6361 tttacaatta aacaagttgt tgctgtcgga attggcgctg ccctctttgt cgtcatcggg
     6421 atgatcaaca ttccaacccc tgttccaaat acaagcatcc agcttcagta tgcggtacaa
     6481 gcgctacttt ctattatttt tggaccgatt atcggtttgc ttgtcgggtt gattggtcat
     6541 gcaatcaagg actctcttgt tggttatggt ctatggtgga cttggattat cgctagcgga
     6601 ctctttggtt tagttgtggg actttttaga aagtacgttc gcgtgatcaa tggtgttttt
     6661 gactggaaag atattcttat ttttaatctc attcaactac ttgcaaatgc ccttgtttgg
     6721 ggtgtcttgg caccacttgg agatgttgtg atttatcaag aagcggcaga aaaagtattt
     6781 gcacaaggga ttgttgcggg aattgccaat ggtgtatctg tagctattgc aggaactctt
     6841 ctcttacttg cctatgcagg aactcaaact cgtgcaggaa gtttgaaaaa ggactaatac
     6901 tcaatgaaaa tcaaagagca aactaggaag ttagccgcag gttgctcaaa acagtgtttt
     6961 gaaattgtgg atagaactga cgaagtcagc tcaaaacatg gttttgaggt tgtagatgaa
     7021 actgacgaag tcagtaacca tacctacggc aaggcgaaac tgacgtggtt tgaagagatt
     7081 ttcgaagagt ataaaatgat ggggaaggct gggcaactag tctttttcga tgtttataga
     7141 ttagttaggc aagtaagcta agataggaat ctttttggcg ctaagatttt aagagaagag
     7201 actgcaagaa gggcttgatt tatgataaaa tagaagtcta agaagcgaat gaagagagta
     7261 agatgaaaga agctataatt gagtggaagg atttctcttt ccggtatgaa acacaacaag
     7321 aaccgacctt gcaagggata gacttgacca tttacaaggg agagaaagtc ttaattgttg
     7381 gaccatctgg atcaggtaaa tctacattgg gtcagtgttt gaatgggatt attcccaata
     7441 tttacaaggg tcagacatat ggagaatttt tgataaaggg tcaagcagcc tttgatatga
     7501 gcatctatga taagtctcat ctggttagca cagttttgca ggatacagat gggcagttta
     7561 ttggcttgtc tgtggcagaa gatttggcgt ttgctctgga aaatgatgtg acagccctag
     7621 atgagatgaa aggtcgtgtt tataaatggg ctgaaaagct ggaccttctt cctttactgg
     7681 atcagcgtcc tcaggatttg tcaggtggac aaaagcagcg agtcagtctg gctggtgtct
     7741 tgattgatga aagtccgatt ctcttgtttg atgagccact cgccaatcta gatcccaagt
     7801 caggtcagga tattatcgaa ttgattgacc agattcataa ggaagagggg acgacgactc
     7861 ttattatcga gcaccgtttg gaggacgttc tgcatcgccc tgtggatcgg attgtcttga
     7921 taaacgatgg tcgtatcctt tttaatggga gccctgacca gttgcttgcg actgatttat
     7981 tgactcaaaa tggaattcga gaaccccttt atctaacgac tctccgtcaa ttaggtgtgg
     8041 acttagtcaa ggaagaacaa ttagcgaatc tggataactt gtctatctca aaaggtcagg
     8101 ttcagttgca gaatgaactg gcaaaagaaa ccccagcatt gcagtcactc tttagactag
     8161 aggaagtatc tttttcttat gatgatagac cgattttaaa atccctacat ttagatatta
     8221 aaaagggtga aaagattgct attgtcggaa aaaatggagc agggaaatca actctagcca
     8281 aggctataag tagctttatt cagacggaag gacgctatct ttgggaaaaa caggatataa
     8341 aaggcgattc tgttgcagag cgggcggaac gagtaggata tgtgctacaa aatcctaatc
     8401 aaatgatttc aaccaatatg atttttgatg aggtggctct agggctccgt ttgcgaggtg
     8461 tggatgagaa ggaaattgaa acgagagtat atgaaacctt gaaaatctgt ggactttatg
     8521 aattccgtaa ttggcctatt tctgccctgt catttggtca gaaaaaacgt gtcaccattg
     8581 cttcaatttt ggtcttagga gctgaaatta ttctcctaga tgaaccgact gcaggtcaag
     8641 atcagaagaa ctatactgag attatggaat ttctcgaaga gttacatcaa aaagggcata
     8701 ccattgtcat gattacccat gatatgcaat tgatgctgga ttattcagac cgggtccttg
     8761 tcatggtgga tggagaattg attgccgata ctgttccagc cagtctgttg agcgatcctg
     8821 agctgttagt aaaagccaat ctaaaagaaa cctccatctt taacttggct aagaaactag
     8881 atgtggatcc actggattta acggcatttt acaaagaaag gagagaggga tgcaagctaa
     8941 attaatcggt taccagcata gagatactgt gattcatcgc ttgtcaggag ctgggaaact
     9001 tctctttttc attctggtat cattggcggc catgattagc tatgatacca gaatgctctt
     9061 gctgattgcc atcttttcag tctttctcct ctatttatca gaaattcgct ttaaagatgt
     9121 ttcctttgta gccgtttttg cgacggtatt tgccgtttta aacgttttga tggtctatct
     9181 cttttctccc gagtatgggg ttggacttta tggagagaga agtgtgattt ggcagggaat
     9241 cggtgtctac actctaacca gccaggagct cttttatctg ctaaatttgg ccattaagta
     9301 tctttgcacc attcctctgg ctattatctt tttgatgaca acccatccta gtcagtttgc
     9361 ttccagttta aatcaaattg gtgtgcccta taagattgct tattctgtca gcctgacctt
     9421 gcgctatatt ccagatttgc aggaagaatt ctttactatc aagatgtctc aggaggcgcg
     9481 tgggatggaa ttatccaaga aagcttctct tatgcaacga atcaaaggca atctgctcat
     9541 tattacgccc ttgattttta gctcgctaga acgcattgat accattgcga ctgccatgga
     9601 gcttcgccgc tttgggaaag agaaaaaacg cacatggtat agttatcagg ccttgaaaaa
     9661 aggagactat attaccttgc tcttggcaac cttgttttta gtagttagtt tactacttat
     9721 cttgcagaat cagggacgat tttacaaccc ttggaaatag cttgaaaaaa ttgaaaaaat
     9781 caagtcattt ctattgacaa tgattctgaa agtgttatac taagaaagta gtttcgctga
     9841 tttacttcaa acctgttgtg aggtaagtta acgatgcctt aaccacgctg tttgctgagc
     9901 ttgactccgg gcagtgtggc tatttttttg caatggtgaa aggaagcaag tcatgacaaa
     9961 tcacattgta ttatttgaac ctcaaattcc acaaaataca ggcaatatcg cgcgtacttg
    10021 cgctgcgacc aattctcccc tccacatcat caagcctatg ggctttccta ttgatgaccg
    10081 caagatgaag cgggctggat tggattactg ggataagcta gagatttatt tttacgagag
    10141 cttagaagat ttcatgtctc agatgaaggg aaaactctat ctgatttcga aattcgcgga
    10201 aaaaatctat tctgaggtgg atttatcgac tgacgaggac cattattttc tctttggacg
    10261 tgaagacaag ggcttacctg aagattttat gcgagaacat cctgagaagg ctcttcgtat
    10321 tcccatgaat gatgaacatg tccgcagtct caatgtgtct aataccgtct gcatgattgt
    10381 ctatgaggct cttcgccagc agaactttgc aggtcttgaa ctcgtccata cctatgaagt
    10441 ggataaattg aaataaaaaa tgaaaatgaa caaaatgctt gcgcttgcaa gcgttttttg
    10501 ttatgataaa agagtcttca gggcagggtg tgattcccga ccggcggtga ctttaactag
    10561 gaaatgatct tttcctttta tactttgttg acaagctttg cctaaccaga agttatgcct
    10621 acagcttgtc gcctagtcta aaagaaaaat ctctatttcc ttctctttaa agaagtccgc
    10681 gagcgcaagc tgatgtggtg agattccaca accgacagta tagtctggat gggagaagac
    10741 gaaagaatag ctttgtctgt tttgatagat ttatagccgg attgtaacca cttgctttgt
    10801 ttttctttaa tagagtaaga gggaactttt gggatataaa aagtgagaat agatagagga
    10861 atcctttcca acttcttctg attttataga aaattggagg aacctgttat gacaaacaca
    10921 cgtcgacttt cgaccattgc aattctatca gccatctcat ttgtgctgat gtactttgac
    10981 tttccgcttt taccagcggc atctttcctc aagatcgaat ttagtatctt gccagtcctt
    11041 gttggtctgg tggtcatgga tttgcctgct gctctaggag tgctcttgct tcgctcacta
    11101 ttgaaattgc ttcttaacag ccaaggagtg aatacttaca ttggtttgcc gatgaatatc
    11161 gtagctttgg gagtttttgt catcgtattt gctttgattt ggaaaaagga acggacaacc
    11221 cttcgtttcc tactaggctc tctagctgga actgttggtt taaccttggc catgttggtt
    11281 ctcaactatg tttatgctgt tcctttgtac gctaagtttg ctaactttga tattggaaaa
    11341 attttgggac tttccaacta cctaatgacc atggtattac cttttaactt gattgagggt
    11401 gtaatctttt ccgtttcatt ctggttgttg tacgtccttt tgaaaccaac cttaaaacac
    11461 tatgaaagat aaacaaacat ttttaatgaa gggcagtttt gcccttttac ttttcgttat
    11521 tcttggctat atggtcaaat tttaccctga aatgttggtc aattttgacc aatcgattca
    11581 gactgccatt cgaggagact taccagatta tttgactatt ctttttcgag ccctcacacg
    11641 tctgattgat atcccagtga ttatcacttg ggttgtcatt acagcttttg tcttttatcg
    11701 taagcgatgg aagatagaaa gtttcttcat gctgggaaat ctggctttgg caggtctttt
    11761 aatcgtgacc tttaaaaata tctaccagcg cccacgacca gctattttac atctggtgga
    11821 ggagaaggga ttttccttcc caagtgggca ttctctagct gtaacgctga tggtagggac
    11881 tttgattgtt attcttagtc agcggattaa agatcaggtc tggagaaaaa tcgtacaaat
    11941 cgtccttggc ctctacctag tcagtgtgct ggtatcaaga gtctatctgg gagttcatta
    12001 cccatcagac gtccttgcca gtctctgtgt gggcttggga gtcctgttta tcgagtttcc
    12061 cttttatgac aagctccgct tccaatggcg atttaaaggc aaacagaagt gagtatcgaa
    12121 ttcccttgag gagaaagaaa tgaaagtcaa tatagcagat cttcatctga ctcaactata
    12181 tttatcagaa aagaagttgc aagatattca gatgctttac cagtcggcag aaacaatcca
    12241 agtcgatcca atcagtattc ttgcctttgg agattgtttg ctgattacag atgggcatca
    12301 cagggcttat caggctttat tggcaggtcg ggatacgatt tctgctgagt gggatagaga
    12361 tggtggtgat gaactatatc atctctatgc gcaagcttgc gaggaaagaa agatttactc
    12421 tgtttttgat ttagaagatc gtatcctagc tcaagatggg tatgaagcaa aatggtataa
    12481 ctggtgtgat ggttttaatc aagcagcaac tcttttgttg aaaaggtagt gcattgtgtt
    12541 ttgttcctta ttataaggtc aatcattgtg aggaagcttt tgcttggtat caggatgtga
    12601 acttggttta cctcgtagat ggggtgaagc ttccttacag tcaagcgacc ttggaagcca
    12661 tgtattccta tttggatcgg catggtgagc ttttttggat tgaagtcaag gagaagggag
    12721 aatggtttcc aattggggat attacactat ctcaggataa tctccccatt gtgattggga
    12781 attccgctta ccaacatcga ggacttggaa aaaagattct aagtgctttg attgaattgg
    12841 ctcgagtaaa aggatggaaa gaattgagag tcaagaaaat ctacacctac aatcatgctt
    12901 ctaggaggtg tttcaagtcg cttggatttg tggaaaatgg agcaacagaa aaaggaatga
    12961 gttttatatt ggaattagtc taatcctgcc actttctccc ccttaacctt tgactattcc
    13021 gcaaaattat cgtaaaataa agagtaaatg ataaaatgag gtcagagtct gttcgctctg
    13081 gcgatagtag tataaatgag gagaaacgct ttgaaattag aagtatttgc tgggcaagaa
    13141 aaaagtgaac tatctatgat tgaggtagcg cgtgctatct tggaacttcg tggtcgcgat
    13201 catgagatgc attttagcga tcttgtaaac gaaattcaaa actaccttgg aacatcaaac
    13261 agcgatatcc gcgaagcttt gcctttgttc tacacagagt tgaactttga cggtagcttc
    13321 atctcacttg gggacaacaa atggggtctt cgttcatggt atggtgtgga cgaaatcgac
    13381 gaagaaatca tcgctcttga agaaaatgac gacgatgaag tagcaccaaa agctaagaaa
    13441 aaacgtgtca atgcctttat ggatggtgat tcagatgcca ttgactacaa tgcagatgat
    13501 ccagaagacg aagatgcata cgaagcagat ccagctcttt catacgatga tgaaaatcca
    13561 gatgatgaaa aaaatgaagt ggaagcttat gatgcagaaa tcaacgaaat cgctccagat
    13621 gacttgggag aagatgtgga tctcaacgaa gacgacgacg agttttcaga tgacgacgct
    13681 gaaaccagcg aggaataaga gttagctatt gacaattatc ctatttttag gtatcatatt
    13741 gtttgggcac ctcttttaga ggtcggggct ccctagttct tagggagcta tttttgtttt
    13801 ttcaagaagt tatcttcttg tattttatac tcaatgaaaa tcaaagagca agctaggaaa
    13861 ctagccgtag gctgctcaaa acactgtttt gaggttgtag ataagactga caaagtcagg
    13921 aacacatatc tacggcaagg cgacgttgac acggtttgaa gagattttcg aagagtatta
    13981 gttgtgaatc tggtgcagtc gtcccagatt attcttatta gtagggtctt gttttctata
    14041 tcccctcgta gttaacaaga ccttgagcat tttagaaaga ggaatctatg tctacgaaat
    14101 atatttttgt aactggtggt gtggtatcgt ctattgggaa agggattgtg gcagcgagtc
    14161 taggccgtct cttgaaaaat cgtggtctca aagtaaccat tcaaaagttt gacccttata
    14221 tcaatattga tccgggaacc atgagtcctt accagcacgg ggaagttttt gtgacagatg
    14281 acggagctga gacagatttg gacttgggtc actatgaacg tttcatcgat atcaatctca
    14341 acaaatattc caacgtgaca actgggaaaa tttacagtga agttcttcgt aaagaacgcc
    14401 gtggagaata ccttggggca actgttcaag tcattcctca tatcacagat gctttgaaag
    14461 aaaaaatcaa gcgtgccgct ctaacgaccg actctgatgt cattatcaca gaggttggtg
    14521 gaacagtagg agatatcgag tccttgccat tcctagaggc tcttcgtcag atgaaggcag
    14581 atgtgggtgc ggataatgtc atgtatatcc atacaacctt gcttccttac ctcaaggttg
    14641 ctggtgaaat gaaaaccaaa ccaacccaac actctgtcaa agaattgcgt ggcttgggaa
    14701 tccaaccaaa tatgttggtt attcgtacag aagagccagc tggtcaagga attaaaaata
    14761 aactggccca gttctgtgat gtggcaccag aagccgttat cgaatcgttg gatgttgaac
    14821 acctttacca aattccactg aacttgcagg cacaagggat ggaccaaatt gtttgtgatc
    14881 atttgaaatt agacgcacca gcagcggata tgacagaatg gtcagccatg gtggacaagg
    14941 tcatgaacct caagaaacaa gttaagattt cccttgttgg taagtatgtg gagttgcaag
    15001 atgcctatat ctcagtggtc gaagctttga aacactctgg ctatgtcaat gatgcagaag
    15061 ttaaaatcaa ttgggtcaat gccaatgatg tgacagcaga gaatgtagca gaactcttgt
    15121 ctgatgcgga cgggatcatc gtaccaggtg gttttggtca acgtggtaca gaagggaaaa
    15181 tccaagccat ccgctatgcg cgtgaaaatg atgttccaat gttgggagtc tgcttgggaa
    15241 tgcagttgac atgtatcgag tttgctcgtc acgttttagg tcttgaaggt gccaattctg
    15301 cagagcttgc accagaaaca aaatacccta tcattgatat catgcgtgat cagattgata
    15361 ttgaggatat gggtggaacc cttcgtttgg gactttatcc gtctaagttg aaacgtggct
    15421 ctaaggctgc tgctgcttat cacaatcaag aagtggtgca acgccgtcac cgtcaccgtt
    15481 atgagtttaa taatgccttc cgtgagcagt ttgaggcagc aggttttgtc ttttcaggag
    15541 tttctccaga caatcgtttg gtagaaatcg tggaaattcc tgaaaataaa ttctttgtag
    15601 cttgtcagta tcaccctgaa ctgtcaagcc gtccaaaccg accagaagaa ctctacactg
    15661 cctttgttac tgcagcggtt gagaacagca attagcaaaa tcagaacctt tgagaaaaat
    15721 ctcagaggtt ttttgcatac gatgatattg cagtatatct gaggtagggg tcctctgtat
    15781 gtacctgcta ccgttgaaat caatagcgac tccctcttgc cctgtgctag tgaatggatt
    15841 tatcagtata ttgaaatgaa ataaaatttg aacaaattaa ttcggaaagc caaatcaatt
    15901 tctagcaaag ttttaggaac tggattgtat agtgaattga aataagatgt gaacatctct
    15961 atcaggaaag
//
LOCUS       DAVGJL010000036        15857 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.036, whole genome shotgun sequence.
ACCESSION   DAVGJL010000036 DAVGJL010000000
VERSION     DAVGJL010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15857
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.036"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..144
                     /locus_tag="VVE42_000943"
     CDS             <1..144
                     /locus_tag="VVE42_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEU3073153.1"
                     /translation="KERSKVENIFAKVKTFKMFSTTYRNHRKRFGLRMNLIAGIINHE
                     LGF"
     gene            complement(223..576)
                     /locus_tag="VVE42_000944"
     CDS             complement(223..576)
                     /locus_tag="VVE42_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073154.1"
                     /translation="MPKTKTLAELADVILWSFDFANDHAHAFFMDNVEWSHADSYFLS
                     FVSDDVEERYTENVYLDSLSVKQKFKFIFDFGDEWRFECQVLREIETEDEEAYLVRSV
                     GTSPEQYPDYDGFDY"
     gene            complement(866..2746)
                     /locus_tag="VVE42_000945"
     CDS             complement(866..2746)
                     /locus_tag="VVE42_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEU3073155.1"
                     /translation="MVRFTGLSPKQTQAIEVLKGHISLPDVEVAVTQSDQASISIQGE
                     EGHYQLTYRKPHQLYRALSLLVTVLAEADKVEIEEQAAYEDLAYMVDCSRNAVLNVAS
                     AKQMIEILALMGYSTFELYMEDTYQIEGQPYFGYFRGAYSAEELQEIEAYAQQFDMTF
                     VPCIQTLAHLSAFVKWGVKEVQELRDVEDILLIGEEKVYDLIDGMFATLSKLKTRKVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSRLVAIEANKACRANQIKEVIVTGWGDNGGETAQFSILPSLQIW
                     AELSYRNDLDGLSAHFKTNTGLTVEDFMQIDLANLLPDLPGNLSGINPNRYVFYQDIL
                     CPILDQHMTPEQDKPHFAQAAETLANIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RQAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEVYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASTIY
                     TT"
     gene            complement(2740..3609)
                     /locus_tag="VVE42_000946"
     CDS             complement(2740..3609)
                     /locus_tag="VVE42_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU3073156.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILDKTSISTPENLEDLLAWLD
                     QRLLEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHGFSWYEALSSYQIPVHLENDAN
                     CVGLSELLAHPELENAACVVIGTGIGGAMIINGRLHRGRHGLGGEFGYMTTLAPAEKL
                     NNWSQLASTGNMVRYVIEKSGHTDWDGRKIYQEAAAGNAHCQEAIERMNRNLAQGLLN
                     IQYLIDPDVISLGGSISQNPDFIQGVKKAVEDFVDAYEEYTVAPVIQACTYHADANLY
                     GALVNWLQEEKQW"
     gene            complement(3693..6338)
                     /locus_tag="VVE42_000947"
     CDS             complement(3693..6338)
                     /locus_tag="VVE42_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEU3073157.1"
                     /translation="MENVVVHIISHSHWDREWYLPFESHRMQLVELFDNLFDLFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQQEAAKWGKSTQIGYFPDTFGNMGQAPQILQKSGIHVAAFGRGVKPIGFDNQV
                     LEDEQFTSQFSEMYWQGVDGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVEGALPEHL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVSTVIDVATCDFKELHPTEGYKKMAALILPSYRVEDL
                     DGHPVEATIEDLGANFEYDLPKDKFRQARIARQVRVTIPVHLAPLSWTTFQLLEGKQE
                     HREGIYQNGVIDTPFVTVSVDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKGHEVLENTACYAKILLKHELTVPVSADEKLEEEQQGIIEFMKREAGRSE
                     ELTNIPLETELTVFVDNPQIRFKTRFTNTAKDHRIRLLVKTHNTRPSNDSESIYEVVT
                     RPNKPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDNTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEFAIECHQAQERFSAYRRAKALQTPFTSLQLARQEGS
                     VVATGSLLSHSVLSIPQVCPTAFKVAENEEGYVFRYYNMSQENVRISEHQQTILDLLE
                     RPYPVHSGLLDPQEIRTEFIKKEEI"
     gene            complement(6429..7709)
                     /locus_tag="VVE42_000948"
     CDS             complement(6429..7709)
                     /locus_tag="VVE42_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEU3073158.1"
                     /translation="MIYSKEIVREWLDEVAERAKDHPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDALLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEKNWKGHHETDHTDLNGWIWERKYEVDSLCYPLQLAYLLWKETGETGQFDETF
                     VAATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFVELDLADSQSVIADAKRLQDEIQEGIENYAYT
                     TNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCSIDDEVYQATRRTILSSEN
                     PYFYQGEYASGLGSSHTFYRYIWPIALSIQGLTTRDKAEKKFLLDQLVACDGGTGVMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            7880..9964
                     /locus_tag="VVE42_000949"
     CDS             7880..9964
                     /locus_tag="VVE42_000949"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEU3073159.1"
                     /translation="MKPLLETIDTRFGTTNKHAFSRGNTLPYTGVPFGMNYFVPQTSD
                     QDGSWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTSQLGGDSLFHRQSSYDTDK
                     ACFQPHYLKLFSLRYQIETQLTPTRYGASIRLKQKQGKALSLYLHATDELTVEQVDKR
                     TLALRQEGKTETNKNSLTMFTALQMNTDILAISQEAGDWRIDLASSQTEMQLATSFIS
                     PSQALINLPQEDFDSCKASAQADWENLLHRFDIIETGEADRTFFDHCLYRLFLFPQTF
                     YEVDESGQAIHMDLATGTVKPGVLFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTGFLPKWLAPDERGMMPGTLLDGIIADSACKDMAPDLEEELFQAMLETASKADP
                     LGINGRHGLAQYQELGYLSTDHHESVSHTLDYAYSDFCIASCAKKLENIEIAETYKAA
                     SQNYRQLFDAETGYMRARDNQGNFHPDFSPYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKACQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFHPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGIPLFDHLRVYLAKENKWLDIHTEQNHSHFNFVKECRLDKTLVSTIQHQDLLKAE
                     QLTFTLSWLPNH"
     gene            complement(10004..11683)
                     /locus_tag="VVE42_000950"
     CDS             complement(10004..11683)
                     /locus_tag="VVE42_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEU3073160.1"
                     /translation="MKKIKPHGPLPSQTQLAYLGDELAAFIHFGPNTFYDQEWGTGQE
                     DPERFNPSQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGK
                     GDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQLKEILSNPNYG
                     NAGKFAEVWMDGARGEGAQKVNYEFEKWFETIRDLQGDCLIFSTEGTSIRWIGNERGY
                     AGDPLWQKVNLDKLGTEAELNYLQHGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLYEFATYRNELYKEDLALGAEVS
                     GPALSADFACRHLTDGLETSSWASDADLPIQLELDLGSPKTFDVIELREDLKLGQRIA
                     AFHVQVEVDGVWQEFGRGFTVGHKRLLRGPLVEAQKVRVMITEAQSIPVLTKISLYKT
                     PGLSKKEVVQELAFAEKSLAVAKGETLHFRIERSESSSSLEAKISIQPGTGVHGVAYQ
                     DEIYVLAFQAGETEKRLTLPTLYFAGDKTLDFYLNLTVDGQLVDQLQVQVS"
     gene            12438..13667
                     /gene="arcA"
                     /locus_tag="VVE42_000951"
     CDS             12438..13667
                     /gene="arcA"
                     /locus_tag="VVE42_000951"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEU3073161.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAIRELLHGIKDNQELVEKTMAGIQKVELPEIPDEAKDLTDLVESDYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPIYGGKVDLVYNREEDTRIE
                     GGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGDLHVYSVTYENEKLKIVEEKGDLAELLAQNLGVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
     gene            13725..14741
                     /gene="argF"
                     /locus_tag="VVE42_000952"
     CDS             13725..14741
                     /gene="argF"
                     /locus_tag="VVE42_000952"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEU3073162.1"
                     /translation="MTNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHY
                     LAGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLV
                     YCGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITED
                     ADEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPA
                     FHDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLY
                     IPKV"
     gene            14906..15853
                     /gene="arcC"
                     /locus_tag="VVE42_000953"
     CDS             14906..15853
                     /gene="arcC"
                     /locus_tag="VVE42_000953"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEU3073163.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVVVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNNGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFAS
                     QRLAELVDADLFIVLTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
BASE COUNT         4472 a         3660 c         3251 g         4474 t
ORIGIN      
        1 aaggagagaa gcaaggttga gaacatcttt gccaaagtaa aaacgtttaa aatgttttca
       61 acaacctatc gaaatcatcg taaacgcttc ggattacgaa tgaatttgat tgctggtatt
      121 atcaatcatg aactaggatt ctagttttgc aggaagtcta ttgaaatact aagcctacac
      181 agactgattc caattgtttt ttagatttca attttaccat tcttaatagt caaaaccatc
      241 ataatctgga tattgttctg gcgacgttcc aaccgaacgt acgagataag cttcttcgtc
      301 ctctgtctcg atttctctca gcacttggca ttcaaaacgc cattcatcac cgaagtcgaa
      361 aataaactta aatttttgtt tgacacttag gctatccaga tagacatttt ctgtgtaacg
      421 ttcttcaaca tcgtcactaa caaagctaag aaagtaagaa tctgcatgac tccactcaac
      481 attatccatg aaaaatgcgt gagcatgatc atttgcaaaa tcgaaactcc ataaaatcac
      541 atctgctagt tccgctaacg tttttgtttt tggaatctat atatgtcggt agcaaccttt
      601 tttaatttga cggaaatgat ccaagcttct acagatgctg gcttttcagc ttctatttct
      661 tgttttacta attttaactg tagcatttga aaccagataa ttcttctgtt tcacgcgaaa
      721 caccctgcgc caagcaaggt gttcttgttt ttatactcaa tgaaaatcaa agagcaaact
      781 aggaagctag ctgcaggctg ctcaaaacac tgttttgagg ttgtagatgg aagctgacgt
      841 ggtttgaaga gattttcgaa gagtattaag tcgtataaat cgtcgacgct gtcgcaatgg
      901 tatgccactg gttggctgtt gtagctgcga aatccttgtc tgcgtagaag tcagtaaatg
      961 gtaggatttc aacttccagc tcgtcgatgc ggtcaagctg gccagccaga taaacctcga
     1021 tacggctttc tgctcgtttg atgcgttgca agagtccgcc catacggatg tcaactgtat
     1081 ccaaaccaaa gaccttgttt tctttcagcc attggtggct aaagagggca tggaagtctt
     1141 caatttggct tctaagttct ggtaattctt gtctggcgat ttgttgtaaa ctttctttat
     1201 catccgcttg gtaggcctga cgaatgcgtc gtcccacatc tactttgcta cttaaaatag
     1261 cattcaactg ggcctgagtt tcaaagagat aagcatagtt tccagctttt tctttaatgt
     1321 tagcaagcgt ctcagcagcc tgagcgaagt gcggtttgtc ctgttcaggt gtcatgtgtt
     1381 gatcaagaat cggacaaaga atatcctgat agaaaacata gcggttggga ttgataccgc
     1441 tgagattgcc tggtaggtct ggtaagaggt tggcaaggtc aatctgcata aaatcctcaa
     1501 ccgttagacc agtattggtc ttgaagtgcg cagacaaacc atctaggtca ttgcgatagc
     1561 tgagttctgc ccagatttgc aagcttggta ggatagagaa ctgggcagtt tcaccaccat
     1621 tgtctcccca acccgttacg atgacttctt taatctgatt ggcacggcag gctttattag
     1681 cctcgatagc cactagacgg ctaaaatggt tgtgaggtgt aaagccaatc cacttccaag
     1741 ctccccctgc aaatgcaagg tcatggctaa tcttgtgatg attgcggaaa ttacggttgt
     1801 atttttcctc gctatcctga taataatccc agtaaaccag agtcacacgg tctttgagac
     1861 ggtctaggta gacacgagtt tcctctggaa tttccacatc acggtcgtac tggccatccg
     1921 ctgacatgag tttgaagaac atatcactcc acatctggca gtggaaacca tatttgtcag
     1981 caatatccag cacgcgctcc aagtgttggc acatgaggag actacgatcc acaacaccgt
     2041 tcaaaatcag gtagcgtccc aaaccaacca agtgggcttc gtccatcccg atattgacct
     2101 tgcgagtctt cagtttagac aacgtggcaa acatgccatc aatcaggtca taaacctttt
     2161 cttcgccaat gagaagaatg tcctctacat cacggagctc ctgtacttcc ttgacacccc
     2221 atttgacaaa ggccgacaag tgggccaagg tctggatgca tggtacaaag gtcatgtcaa
     2281 actgttgagc ataggcttcg atttcctgca actcctctgc tgaataagct ccacggaaat
     2341 agccaaagta aggctgccct tcaatctggt aagtgtcttc catgtaaagc tcaaaggttg
     2401 agtagcccat gagagccaat atctcaatca tctgcttggc agaagccaca ttcagcaccg
     2461 catttcgaga acagtcaacc atgtaagcca aatcttcgta agctgcttgt tcctcaatct
     2521 ctactttatc agcttctgct agaactgtta ccaacaagga taaggcacga tagagttggt
     2581 gaggtttgcg gtaggtcaat tgatagtgac cttcctcacc ctggatagag atagaggctt
     2641 ggtcagactg agtgacagcc acttccacat ctggtagaga aatgtgacct tttaaaacct
     2701 caatagcttg cgtttgtttg ggactaagtc ctgtaaatct taccattgct tttcctcctg
     2761 tagccagttg acaagggcac cgtagagatt ggcatctgcg tgataggtgc aggcctggat
     2821 aactggtgcg accgtgtatt cttcgtaggc atcgacaaaa tcttcaacag ccttcttgac
     2881 accttggata aaatctggat tttgactgat agagccaccc agactgatga catctggatc
     2941 gatcagatac tggatattga gcaagccttg cgccagattg cggttcatgc gctcaatggc
     3001 ttcttgacaa tgagcattac cagctgcggc ctcttggtaa atcttgcgac cgtcccaatc
     3061 agtatgacca gatttttcaa tcacgtatcg taccatattc ccagttgatg ctagttgcga
     3121 ccagttatta agtttttcag caggggcaag ggttgtcatg tagccaaatt ctccacccag
     3181 accgtggcga cctcgatgaa gtctaccatt gataatcatg gctccgccaa tccctgtccc
     3241 aatcacgaca caggctgcat tttcaagctc tggatgagct agtagttcac tgagtccaac
     3301 gcagttggca tcattttcca gatggacagg aatctgataa gagctaagcg cctcatacca
     3361 agaaaatccg tggatgtagg gtaccgcact gaagccatca atcacacctg tctcttgatt
     3421 gaccgctcct ggaacgctca tagcaatccc actgtaatcc tgttctaaca agcgttgatc
     3481 tagccacgct agtaaatcct ccaagttttc aggcgttgaa atacttgtct tatccagtat
     3541 tttcccatca ggagtcagac tggcaaactt aatcccagtc cctccgatat caatcgttgc
     3601 aatggtcatt gtttttacca taaaaagggg cgaatcagca gttagtcctt actttttcgc
     3661 ccctcctttc ttttgatgtt tactttttga aattaaattt cttctttttt gatgaattct
     3721 gtacgaatct cttgtggatc caatagtcct gaatgaactg gatatggtcg ctcaagtaag
     3781 tcaagaatgg tttgttgatg ttctgatatg cgcacatttt cttgactcat attatagtaa
     3841 cggaagacat agccttcttc attttccgcc actttaaagg ctgttggaca aacttgcggt
     3901 atgctgagaa cagaatggct caagaggcta ccagtcgcaa ccacgcttcc ttcctgtcta
     3961 gcaagctgaa ggctggtaaa cggtgtctgc aaggctttgg cacgacgata ggctgagaag
     4021 cgttcttggg cttggtggca ttcaatcgca aattcgactt caaactcccg caagcattgt
     4081 gcttctggcg ttgggaagta gccccagtca cctagctcac ctgatgcacg caaaatggtc
     4141 acggcaatgg tgttatcccc aaggatttcg tattcattca atcccttatt ggatacagtc
     4201 accccttttt catcgtcata cagactgaca aaagcttgtt ggtgttgagg attttcagga
     4261 ttttcccatg aagcagctgg tttgtttggt cgtgtcacca cctcatagat actttcagaa
     4321 tcattgcttg gacgcgtgtt atgagtcttg accaagagac ggatacggtg atccttggca
     4381 gtgttagtaa agcgagtctt gaagcggatt tgtggattgt caacgaagac agtcaactca
     4441 gtttccagag gaatgtttgt caattcttct gaccgtccag cctcacgctt cataaactcg
     4501 atgatacctt gttgctcttc ttctagcttt tcatccgcgc tgacaggcac ggtcaattca
     4561 tgtttgagca agattttagc atagcaagct gtgttttcca agacctcgtg gcccttaagc
     4621 tctgcaaaga ttggctctgt tccttttggt tggaaataga tatactcgtt tccgatgtcc
     4681 ccacggtctt caaagcggat aaagtcttca taggcttcgt gagttgtctt gtcatagact
     4741 gtgatgttgt catccacact caccgttacg aatggtgtat caatcactcc gttttggtaa
     4801 ataccctcac ggtgttcttg ttttccttcc agcaattgga aggttgtcca agaaagcggc
     4861 gctaggtgaa ctggaatggt cacgcgcact tgacgagcaa tacgagcttg gcggaacttg
     4921 tcttttggta aatcgtactc aaaattagct ccgaggtctt cgattgtagc ctctacagga
     4981 tgaccatcca agtcctccac acggtaactt ggcaagataa gagcagccat ctttttgtag
     5041 ccttctgttg ggtgcaattc cttgaaatca caagtcgcca catcaatcac tgtgctgaca
     5101 gtatcgacct tatcatgcaa gcctgtgtta atgacagtaa agagatagtc actttgagcc
     5161 ttatccgtag caattttacc cttccactcg ttgagcaagt tacttttaac aaagtttcct
     5221 acttggttga ccttggcaaa acgcgtttcc atctcgcggt gaacttcgtc cacgctacag
     5281 ccacagatac tatcatgtgg cgcattctgc aaaagtgttt tccaagcata ggtcaactgg
     5341 tccttgtggt tgtgtccacc agtgataata gtcaagggtt ctaccacttg ctctaggagg
     5401 ttgctatttt cttggaaggc ttgttttagg taaatgcggg atgaagaagt gttggcaagt
     5461 gtgtaccagc catctgtttc ctgactggtc aactcacctg taacagttga taagtgttca
     5521 ggaagcgcac cttctacagc ttgaacatat tcatcaaaag aactatgaac aaagattaca
     5581 tctgggaaga gttcatttgc cacacgaatg gcttcgctca gatttttctg tacaggctgg
     5641 tggtcacagc cgttcatcat caaccattgg ttggtcgaag cgtaggcacg cacatctgac
     5701 aatttttgtt tccagaaggt caaggcctca tctttgtcaa ctgggatttc attcccatta
     5761 ctgtaccagt tggcaaagag aatacctaaa acacgactac catccacacc ctgccagtac
     5821 atttctgaaa actgggatgt aaactgctca tcttcaagga cttggttgtc aaatccaatc
     5881 ggcttcacac cacgaccaaa ggccgccacg tgaatgcctg atttttgaag aatttgaggc
     5941 gcttgtccca tatttccaaa ggtatctgga aagtagccaa tctgggttga tttaccccat
     6001 ttggcagctt cttgttgacc aatcaaggta ttgcggacat tggcttcact ggagatcaaa
     6061 taatcatcct gcaagatgta aaagggacca attttaagct tgccctcgtc aatgtagcgt
     6121 tggaccttat cacgattttc agggcgaatt tgtaagtagt cgtcaaggac aatagtttgt
     6181 ccatccaagt ggaaactctt gaactcaggg tcattttcaa agagatcaaa gagattgtca
     6241 aacaattcca ccaactgcat acgatggctt tcaaaaggca agtaccactc acggtcccag
     6301 tgactatgtg agataatatg tacaacaaca ttttccatga agtaaaacct cattctaaat
     6361 ttaaatttta acgttttaaa tgtaaacttg tgattctgat aagaatcggt tgagctaaag
     6421 cgagcccctt agcgaatatc caagtaatcc aagaccaact cacagaacat catgttagcc
     6481 caggagaacc attcacgaga gtagagggtc ggatcatcta cgtggaagct ttcgtgcatg
     6541 acacctgtac caccatcgca ggcaaccagc tgatccagca agaatttttt ctctgcctta
     6601 tctcttgttg tcaatccttg gatagaaagg gcgattggcc agatatagcg atagaaggta
     6661 tgagaacttc cgagaccgct agcgtattct ccttggtaga agtatggatt ttcagagctc
     6721 agaatggtac ggcgagtggc ttgatacact tcatcatcga tcgaacagta gcccagatag
     6781 ggcgcagcta gtagacttgg tacatttgga tcatccatga tgctggcatt tcctaggcca
     6841 tctacttcaa aagcgtaaat cttttcgccc ttgctgttgg tggtgtaagc gtagttttcg
     6901 attccttctt ggatttcatc ctgaagacgc ttggcatcag caataacact ctggctatca
     6961 gctaggtcta attcgacaaa gatttcttgc acataaccca agactactac ggcaaacata
     7021 ttagacggaa tcaagtaact atactgacag caatcgtcac tcggacgaaa agctgaccaa
     7081 gtcatacctg tcactgcaaa gtcaggtcca aagccatcat ttaccaaggt gtcttcctta
     7141 cggtccgtat ctcgaacaaa acgataagga gagttcttgt ggtcttgttc aactgtccat
     7201 agatggagaa tttccttagt cgctgcgaca aaagtctcat caaactgacc agtctcgcca
     7261 gtctctttcc agaggagata agccaactgc aaaggatagc aaagtgaatc cacctcatac
     7321 ttgcgctccc agatccagcc gttaaggtct gtgtggtcag tctcgtggtg ccctttccag
     7381 ttcttctcaa tgttgaagga gttagcatag ggatccttga gtaccaaggt catctgacgt
     7441 ttgaccaaac ctgcaatggt ctgacgcagg agggcatctc ttttagctac atgaaggtag
     7501 ggtctgagtt gggctgtcga atctcgaagc cacatggcag gaatatcccc agtcaagaca
     7561 aaagttgaac catcttctaa gatttcaacc gtattgtcca aggtatcggt gtagcaacgc
     7621 tcgaaaacat ccacccactc tggatggtcc ttagcccgct ctgctacttc atctagccat
     7681 tctctaacaa tttctttcga ataaatcatc gtgcaattcc ctctttcaaa caagtttcat
     7741 gttcagtata tagcttttga gacagaaaat acatacacaa atgatagtca tttttctaca
     7801 aaatagttat ttttgaaact ccttttctaa aggccctctt tttctgatat aatgtagaaa
     7861 aacagctaaa ggaaagacta tgaaaccact acttgaaacc atcgataccc gctttggaac
     7921 tacgaacaag catgcctttt ctcggggaaa taccctgcca tacacaggcg tgccttttgg
     7981 gatgaattac tttgtgcccc agaccagtga ccaggatgga tcttggtttt ttgatccgca
     8041 tctgcctatc tttcagggga ttcgattaac tcaccagccc agtccttgga ttggggacta
     8101 ctcttggctc cttctgacac ctgtcaccag tcaactgggt ggagacagcc tctttcaccg
     8161 ccagtcttct tatgatacag ataaagcttg cttccaacct cactatttga agctcttttc
     8221 tctgcgttat cagattgaaa cccagctcac accgactcgc tacggtgctt ctattcgttt
     8281 gaagcaaaag caaggcaaag ccctctccct ctatcttcac gcaacagatg aactgacagt
     8341 agagcaagta gataagcgaa ctcttgccct gcgacaagaa ggtaaaactg aaaccaacaa
     8401 aaattcgcta acgatgttca ctgccctgca aatgaacacg gatattcttg ctatcagcca
     8461 agaagctggg gactggcgaa ttgacttagc aagtagtcaa accgagatgc aactagccac
     8521 ttctttcatc tctccttctc aagctctaat caatctacct caagaagatt ttgatagctg
     8581 taaagcaagt gcccaagcgg attgggaaaa tctcctccat cgttttgaca ttatagagac
     8641 aggagaggct gaccgaacct tctttgacca ctgtctctac agactcttcc tcttcccaca
     8701 gactttttat gaggttgatg agtcagggca agccatccac atggatctgg ctactggtac
     8761 tgtcaagcct ggtgtcctct ttagcaacaa tggtttctgg gataccttcc gcaccacctt
     8821 ccccctcttt gcccttatca taccggagca ctatcaacgc tttttagaag gtttcctcaa
     8881 tagctaccgc gatactggtt tccttccaaa atggctggct ccagatgaac ggggtatgat
     8941 gccaggtaca cttttagacg gtattatcgc agatagcgcc tgcaaggaca tggcccctga
     9001 cctagaagaa gaactcttcc aagccatgct cgaaacagcc agcaaagccg accctctcgg
     9061 catcaatggc cgccacggac tagcccaata ccaagaacta ggttacctct ctaccgacca
     9121 ccacgaaagt gttagccata ctctagacta tgcctatagt gacttttgta tcgccagctg
     9181 tgccaaaaaa ctagagaaca tagaaatcgc tgaaacctac aaggctgctt cacaaaatta
     9241 ccgccagcta tttgacgctg agacaggtta catgcgagca cgagacaatc aaggaaactt
     9301 tcaccctgac ttctctcctt atagttgggg gcgagactac gctgaatgct ctgccattca
     9361 agctacttta ggcgttctcc acgacatccc tggcttaatc caactgatgg gtggaaaaga
     9421 aacctttagc aactatcttt tgaaagcctg tcaagatgct cccctctttg aaacaacagg
     9481 ctatggttac gaaattcacg aaatgagcga gatggctact gctccttttg gacaaatagc
     9541 tatttccaac cagcctagtt tccacattcc ttatctcttc cgctacagcg attaccctga
     9601 ctacactgcc cttcttatca agacgctccg tcagaaagct tttcacccaa gttgggaagc
     9661 ctatcctgga gatgaagaca atggtagtct ctctgcttgg tacatctggt cagctctcgg
     9721 attttatcca acctgtccag gaaaaccaag ctatgatctc ggaattcctc tctttgacca
     9781 tctacgagtc tacctagcta aagaaaataa atggctagat attcatactg agcaaaacca
     9841 cagccatttt aactttgtca aagaatgccg actggacaaa acactcgtat caactattca
     9901 acaccaagac ctcctaaaag ctgaacaact aactttcacc ctcagctggc taccaaatca
     9961 ctaataacca aaagaacctt tgcatcgcgc aaaggttctt cttttatgaa acttggactt
    10021 gaagttgatc cacaagctga ccatccaccg ttaggttcag atagaaatcc aaggttttat
    10081 ctcctgcgaa atacaaggtt ggtagcgtca gccttttttc agtctcacca gcttgaaacg
    10141 caaggacata aatctcgtcc tgataggcga caccatggac acctgtcccc ggttgaatcg
    10201 aaatcttagc ctctaaagag gagctacttt cactacgttc aatcctaaaa tgaagagtct
    10261 ctccctttgc cacagctagg cttttttctg caaatgctag ttcctgaaca acttcttttt
    10321 ttgataaccc aggagtttta taaagggaaa tcttggtcaa tacagggata gattgtgcct
    10381 cggtaatcat cacgcgcacc ttttgtgcct ctactagcgg acctcgcaag aggcgcttat
    10441 gaccaactgt gaagcccctg ccaaactcct gccagacacc atccacctct acttgcacat
    10501 gaaaagcagc gattcgttgc cctagcttca aatcttctct taactcaatt acatcaaaag
    10561 ttttaggaga acctaaatcg agttctaact ggatgggcaa gtctgcatcg cttgcccaag
    10621 agctggtctc aaggccgtct gtcaaatggc gacaagcaaa gtctgcggaa agagctggac
    10681 cagatacctc agctcccaga gccaaatctt ctttatagag ctcattgcga taggtcgcaa
    10741 attcataaag tcgttcaata tcctttgcat caaagagccc agcttgattc ggcggaatat
    10801 taagcaagag tggagttcct cgccctactg agtgaaagta gatttcgacc aactcctcga
    10861 gagacttagg atcctgatcc tcatggtaga accagcctgg acggatggaa acatctgcct
    10921 ctccgattga aaaaatcgtg cccgagggat ccccgtgctg aagatagttc aactctgctt
    10981 ctgttcctag tttatcaaga ttcacctttt gccacagtgg atcacctgca taccctcgtt
    11041 cattgccaat ccagcggata ctggtgcctt ctgttgaaaa aatcaagcaa tcgccctgca
    11101 ggtcacgaat ggtttcaaac catttttcaa attcataatt aaccttttgc gcgccctctc
    11161 ctctggcacc atccatccaa acctcagcga acttaccagc attcccatag ttaggatttg
    11221 ataagatttc cttcaactga gccagataat aggcattgta gtccgcttct cggtccacat
    11281 gatagagggg actatgggca tcccacggtg acaggtagac ccccatatcc atatcaaact
    11341 ctgtggcagc ttgggatact tcaaggagca agtcgccctt tcctctcctc caaggactga
    11401 ccttaaccga ataatctgtg tgagctgtcg gataaaggac aaagccatcg tggtgcttga
    11461 ccaccaaaat caactttttg aagcccgttt ccttgagcac acgaacccac tcacgcgcat
    11521 ccaactgact cgggttaaag cgctcaggat cctcctgtcc agtcccccat tcttggtcat
    11581 aaaaggtatt aggaccgaag tggataaaag ctgctagttc atctcccaga taagctagct
    11641 gagtctgact tggtaacggt ccatgcggtt tgattttctt catcgttata tatctccttc
    11701 tttagcaact tctcttgttc acaaggttat ccacaggttg tggataataa accatgatct
    11761 aacagccagc tctttccaac tccaactggt aggctcagga tttttctcct caatattccc
    11821 tcttcaaaca gctctgtcat atggttctgc taacacctct cagaatgcta ctctccataa
    11881 ttattctcag gcttctttca ggtcattgtg tgaagaccat tcactctcca gacttttcca
    11941 cagattgtga ataagtgatt attctaaact atcacctcgt cgactataac aaaaaataaa
    12001 aagagggtca ataagataat tatctcttcc taaatactca gaaaagattc tataatttgt
    12061 acaattgaac aagaaatgct tctccaaaat ctatcttcaa aagaatgttg catcttttta
    12121 tcctttcaca caaacatttc tctttataca gatccaagtg ataaccagat gataattgga
    12181 cactttttgg tcatttgtat attttttgaa aaatttttta aaaaaatttt atatcctttt
    12241 tatagaagtt tatgcatttg tatctatgta ttctatccta tgttatgtat aataacataa
    12301 aattgtgcta aacatcataa tttatattat tttccgagaa attatagcca ttgacattac
    12361 ctaggaattg cgttataaca tacatgtaag cggtacccaa aatggtgcca agtcaaaatt
    12421 tttaaggagg aaaatacatg tcttcacatc caattcaggt cttctcagaa attgggaaac
    12481 tgaaaaaagt tatgttgcac cgtccaggca aggagttaga aaacttgttg ccggactatc
    12541 ttgaaaggct tctttttgat gatattcctt tcttggaaga tgctcaaaaa gaacatgatg
    12601 catttgccca agctcttcgc gatgaaggaa ttgaggttct ctacctagaa caactcgctg
    12661 ctgaatcatt gacctctcca gaaatccgcg atcaatttat cgaggaatac ttagacgaag
    12721 ccaacatccg tgatcgtcaa accaaggttg ctattcgtga attgcttcac ggcatcaagg
    12781 acaaccaaga attggttgaa aaaacaatgg ctgggattca aaaagttgaa ttgccagaaa
    12841 ttcctgacga agctaaagat ctaactgact tagttgaatc agattatcca tttgcaattg
    12901 acccgatgcc aaacctctat ttcactcgcg acccatttgc aacaattgga aacgccgtat
    12961 cgcttaacca catgtttgca gacactcgta accgtgaaac actctacggt aagtatatct
    13021 tcaaatacca cccaatctat ggcggaaaag tggatttggt ctacaaccgt gaagaagata
    13081 cgcgtatcga aggtggagac gagctagttc tttctaaaga cgtccttgca gtaggtatct
    13141 ctcaacgtac agacgcagct tctatcgaaa aacttttggt caacatcttc aagaaaaatg
    13201 ttggcttcaa gaaagttttg gcctttgaat ttgctaacaa ccgtaaattc atgcacttgg
    13261 atactgtctt cactatggta gactatgaca agttcactat tcacccagaa atcgaaggcg
    13321 accttcacgt ttactcggtt acttacgaaa acgaaaaact taaaatcgtt gaagagaaag
    13381 gtgacttagc tgaacttctt gctcaaaacc ttggtgtaga aaaagttcat ttgattcgtt
    13441 gcggtggtgg caatatcgta gcagctgcgc gtgaacaatg gaacgacggt tctaacactt
    13501 tgaccatcgc acctggtgtg gtagttgttt atgaccgcaa taccgtgacc aataagattt
    13561 tggaagaata cgggcttcgc ttgattaaga ttcgcggaag tgaattggtt cggggccgtg
    13621 gtggacctcg ttgtatgtct atgccatttg aacgtgaaga agtgtaatcg ctgttcggca
    13681 ttcgtcaata gaaaatgtaa aaaatagaaa gaggaaataa taaaatgaca aattcagtat
    13741 tccaaggacg cagcttctta gcagaaaaag actttacccg tgcagagtta gaatacctta
    13801 ttggtctttc agctcacttg aaagatttga aaaaacgcaa tattcaacac cactaccttg
    13861 ctggcaagaa tatcgctctc ctatttgaaa aaacatctac tcgtactcgt gcagccttta
    13921 caactgcggc tatcgacctt ggtgctcacc cagaatacct cggagcaaat gatattcagt
    13981 tgggtaaaaa agaatctact gaagatactg ctaaagtatt gggacgtatg tttgacggga
    14041 ttgaattccg cggattcagc caacgtatgg ttgaagaatt ggcagaattc tcaggcgttc
    14101 cagtatggaa cggtctaact gacgaatggc acccaactca aatgctcgct gactacttga
    14161 ctgttcaaga aaacttcggt cgcttggaag gcttgacatt ggtatactgt ggtgatggac
    14221 gtaacaacgt tgccaacagc ttgctcgtaa caggtgctat ccttggtgtc aatgttcaca
    14281 tcttctcacc aaaagaactc ttcccagaaa aagaaatcgt tgaattggca gaaggatttg
    14341 ctaaagaaag tggcgcacat gttctcatca ctgaagatgc tgatgaagca gttaaagatg
    14401 cagacgttct ttacacagac gtttgggtat caatgggtga agaagacaaa ttcgcagaac
    14461 gtgtagctct tcttaaacct taccaagtca atatggactt agttaaaaaa gcaggcaatg
    14521 aaaacttgat cttcctacac tgcttgccag cattccacga tactcacact gtttatggta
    14581 aagacgttgc tgaaaaattt ggtgtagaag aaatggaagt aacagacgaa gtcttccgca
    14641 gcaagtacgc tcgccacttc gatcaagcag aaaaccgtat gcacactatc aaagctgtta
    14701 tggctgctac acttggtaac ctttatattc ctaaagtata attttagata ataaaccgtc
    14761 taccaacagc tatgagggct gcgactaata gctttagtcc ggtcctcttt tatgtaatgg
    14821 taatctatta tttcttataa aatatgtgaa aaatcattaa attgaaatct aaacgcattc
    14881 tattgagtgt gataaaggag aatttatggc aaatcgtaaa attgtagtag ctttgggagg
    14941 aaatgcgatt ctttcttctg acccatcagc aaaggctcaa caagaagctt tagttgaaac
    15001 agctaagcat cttgtaaaat tgattaaaaa tggagatgat ctgattatca ctcacggtaa
    15061 tggacctcaa gttgggaatc tcttgctcca acatttggca tcagactctg aaaagaaccc
    15121 tgccttccca ctcgactcac ttgtcgctat gacagaaggt agcatcggtt tctggttgaa
    15181 aaatgctttg caaaatgctc tcttggatga aggcatcgaa aaaaatgttg cctctgttgt
    15241 aacgcaagtt gtcgtagata aaaatgatcc agcttttgtt aacttgagta aaccaatcgg
    15301 tcctttctat tcagaagaag aagcaaaagc agaagccgaa aaaagcggag cgactttcaa
    15361 ggaagatgct ggccgtggct ggcgtaaggt cgttgcctca ccaaaacctg ttgacatcaa
    15421 agaaattgaa accatccgta ctcttttaaa taatggtcaa gtcgtcgtag ctgcaggtgg
    15481 tggcggtatt cccgtcgtca aagaaaacaa tggacatttg actggtgtcg aagcggttat
    15541 tgataaagac ttcgcttccc aacgtttggc agaattggtt gatgcagacc tcttcatcgt
    15601 tttgacaggt gtagattatg tatttgttaa ctacaacaag ccaaaccagg aaaaattgga
    15661 acatgtgaat gttgcccagc tggaagaata tatcaaacaa gatcagtttg caccaggtag
    15721 catgcttcca aaagtagaag cagctatcgc ttttgtcaat ggtcgtccag aaggaaaagc
    15781 agttattact tcccttgaaa atctaggcgc cttgattgaa tctgaaagcg gaacaattat
    15841 tgaaaaagga taagttg
//
LOCUS       DAVGJL010000037        15834 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.037, whole genome shotgun sequence.
ACCESSION   DAVGJL010000037 DAVGJL010000000
VERSION     DAVGJL010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15834
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.037"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            14..562
                     /locus_tag="VVE42_000954"
     CDS             14..562
                     /locus_tag="VVE42_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU3073164.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            672..1571
                     /locus_tag="VVE42_000955"
     CDS             672..1571
                     /locus_tag="VVE42_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEU3073165.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDTLPVLSHLFSLTGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACPGRRVHATPSWTVLYVGIAVAALTYPLVGIIEIAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTSLPTWVLIVIIL
                     ASQSFFFFVLTRLPNILKQGFQPAFSALTFPTIITATSLKMAQGILKLPFLDYLVLAE
                     TIICLTILFFVLGAYLIWLRKKV"
     gene            complement(1596..2870)
                     /gene="serS"
                     /locus_tag="VVE42_000956"
     CDS             complement(1596..2870)
                     /gene="serS"
                     /locus_tag="VVE42_000956"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEU3073166.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVDAAVLNEMKEIDAKRRNILVK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEVKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGADEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLTNYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSATKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEALRPYMGGAEVIKP"
     gene            complement(3084..3455)
                     /locus_tag="VVE42_000957"
     CDS             complement(3084..3455)
                     /locus_tag="VVE42_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEU3073167.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLIGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGRKISRHFEVFTDKGKFLFASKDSGAILKIARKKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            complement(3458..4822)
                     /locus_tag="VVE42_000958"
     CDS             complement(3458..4822)
                     /locus_tag="VVE42_000958"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEU3073168.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKSQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPTEENEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREVGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRQLTPIKEEEILRQLVQKAKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGE
                     SKED"
     gene            5147..5932
                     /locus_tag="VVE42_000959"
     CDS             5147..5932
                     /locus_tag="VVE42_000959"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEU3073169.1"
                     /translation="MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            6026..6460
                     /locus_tag="VVE42_000960"
     CDS             6026..6460
                     /locus_tag="VVE42_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU3073170.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGRLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            6460..7434
                     /locus_tag="VVE42_000961"
     CDS             6460..7434
                     /locus_tag="VVE42_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEU3073171.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGKELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANDRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIPLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            7494..7718
                     /locus_tag="VVE42_000962"
     CDS             7494..7718
                     /locus_tag="VVE42_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU3073172.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQAK"
     gene            7837..8811
                     /gene="fabK"
                     /locus_tag="VVE42_000963"
     CDS             7837..8811
                     /gene="fabK"
                     /locus_tag="VVE42_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEU3073173.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     ISIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVD
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWAGVVRND"
     gene            8804..9724
                     /gene="fabD"
                     /locus_tag="VVE42_000964"
     CDS             8804..9724
                     /gene="fabD"
                     /locus_tag="VVE42_000964"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEU3073174.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPVVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVALVAKRGAYMEEAAPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTALLEPASQKLAETLAQVS
                     FSDFTCPLVGNTEATVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDQTAHLAHVEDQASLVALLEK"
     gene            9758..10489
                     /gene="fabG"
                     /locus_tag="VVE42_000965"
     CDS             9758..10489
                     /gene="fabG"
                     /locus_tag="VVE42_000965"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEU3073175.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQAGANIVLNSRGAISEEL
                     LAEFSNYGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGAFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVVAIDGGLSM"
     gene            10511..11746
                     /gene="fabF"
                     /locus_tag="VVE42_000966"
     CDS             10511..11746
                     /gene="fabF"
                     /locus_tag="VVE42_000966"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEU3073176.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFIKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAVKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEANVVYGQGLEKEIPYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            11749..12234
                     /locus_tag="VVE42_000967"
     CDS             11749..12234
                     /locus_tag="VVE42_000967"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEU3073177.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLATPSPVAPTSAPAETVAEEVPAPAEASVATEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            12231..12653
                     /gene="fabZ"
                     /locus_tag="VVE42_000968"
     CDS             12231..12653
                     /gene="fabZ"
                     /locus_tag="VVE42_000968"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEU3073178.1"
                     /translation="MIDIQGIKEALPHRYPMLLVDRVLEVSEDTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVLIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKRRGTIAVVEAKAEVDGKLAASGTLTFAIGN"
     gene            12665..14032
                     /gene="accC"
                     /locus_tag="VVE42_000969"
     CDS             12665..14032
                     /gene="accC"
                     /locus_tag="VVE42_000969"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEU3073179.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVEKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASSNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNADFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            14069..14935
                     /locus_tag="VVE42_000970"
     CDS             14069..14935
                     /locus_tag="VVE42_000970"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEU3073180.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNAGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            14932..15699
                     /locus_tag="VVE42_000971"
     CDS             14932..15699
                     /locus_tag="VVE42_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEU3073181.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDNLKRNFGQPHPEGYRKALRLMKQAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGTRAMEAAELMKITSHELLEMDVVDKVISEVGLSS
                     KELIKSVKKELQTELARLSQKPLEELLEERYQRFRKY"
BASE COUNT         4516 a         3017 c         3718 g         4583 t
ORIGIN      
        1 aaagaggtat cttatgacaa catttactat ccatacagta gagtcagcac cagcagaagt
       61 gaaagaaatt cttgaaacag tagaaaaaga caacaatggc tatattccca acctaatcgg
      121 tctcttggcc aatgccccga ctgttttaga agcctaccaa attgtctcat ctatccaccg
      181 tcgcaacagc ctgacacccg ttgagcgtga agtggtgcaa atcacggcag ccgtgaccaa
      241 tggttgtgcc ttctgtgtcg caggtcacac agccttttcc atcaaacaaa tccagatgaa
      301 tgatgacttg attcaagctc ttcgcaatcg tactccaatt gaaacagatc ctaaattgga
      361 taccctagct aagtttacct tggcagttat caataccaag ggtcgtgtag gagatgaagc
      421 cttgtctgag tttttagaag ctggctacac tcaacaaaat gccttggatg tggtttttgg
      481 tgtcagccta gcaatcctct gtaactatgc caacaactta gctaatacac caattaatcc
      541 agaattgcaa ccttatgcct aattcatatc tgagtaaaat gaagtctgaa ataagcggac
      601 ttcgtttttt taaatcctca tttaagcgct tttatgttat actgaaacta acatgattat
      661 tggaggttag gatgaaaaaa ctccccttag tattttcagg ttgtttgcta ggtttggcag
      721 gagctggaaa tcttatttta gatacgttgc cggttctatc ccatcttttt agtctgactg
      781 gtttggtttt atggatttac tttctaattc tgcatctctt taattggaaa gaaaccaagc
      841 aagaattgac caagccccct cttttgtcag gaatggcaac ctttcctatg gctgggatga
      901 ttttatcgac ctatgtcttt cgcgtattct cttatcttcc tttggtagca caagggattt
      961 ggtggttttc atttctctta gatttgacct tgattgctgg ttttaccatc aagtttgctt
     1021 gtccagggcg gagggttcat gccactccaa gttggacggt tctctatgtg gggatagcag
     1081 tggctgcctt gacctatcct ctggtaggta ttatcgaaat tgcctatgcg accttgagtt
     1141 ttggttttct cttgaccttc tatctctatc cccttattta tagcgattta aagaaacatc
     1201 cactcccact agccttgctt ggacaagaag gaatctactg tgctcctttc tctctactct
     1261 tggcttctct agttcgagta ggaggaacca gcctgccgac ttgggtcttg attgtcatta
     1321 ttttggcttc tcaatccttc tttttctttg ttttaactcg tctgcccaac attttaaaac
     1381 aaggttttca accagccttc tcagccctca ccttcccaac cattatcaca gcgacctcgc
     1441 tcaagatggc tcagggaatt ttgaaacttc catttctgga ttacctggta ttggctgaaa
     1501 ccattatatg cctaactatt ttattctttg tactgggtgc ttatctgatt tggttacgaa
     1561 aaaaggtcta gctagaaata gctaaacctt attttttatg gtttgatgac ttcagctcca
     1621 cccatgtatg gacgaagtgc ttctgggatg gtcacagaac catcttcatt ttggtaattt
     1681 tcaagaattg cagccactgt acgtccaact gcaagtccag aaccgttcaa ggtatgaagg
     1741 agtttcacct tgccatctgc ttcatcacgg taacggattt gggcacgacg ggcttggaaa
     1801 tcttctgtgt ttgaacagct tgagatttca cggtaattgt tttgtgctgg aatccacact
     1861 tccaagtcgt aagtcttcgt agctgagaag cccatatctc cagtagagag agcaacgaca
     1921 cggtatggaa ggttgagttt ttgaagaatg ttttcagcgt tggctgtcat tttttccaat
     1981 tcttcgtaag attcttctgg tttggcaaat ttgaccattt caaccttgtg gaattggtgc
     2041 aaacggatca agccacgcgt atcacgaccg gcagaaccag cctcagaacg gaatgacgga
     2101 ctcatggcag tgaagtagat tggaagatct ttgccgtcta agatttcatc acggtagtag
     2161 tttgtcagag gaacttcagc agttggaatc aagacaaagt tggtatcgct gagttcaaaa
     2221 gtatcttcct taaattttgg atactgacca gtaccaaaca tagaatcatg gttgactatg
     2281 taaggtgtga tgacttcagt atagccttct tttccatgtt catccaacat aaagttgtag
     2341 atagcacgtt ccaaacgagc accgaggcct ttatagaaga ggaagcgagc gcctgttacc
     2401 ttaccaccgc gttcccagtc aaggatacca aggtcttcac cgagatccca gtgagcttta
     2461 ggttcgaagt caaactcgcg tggagtaccc caacggcgaa cttccacatt gtcgtcttcg
     2521 tcagccccaa caggaacgct gtcagctggg atatttggaa gagtcgttgt aaattctgtc
     2581 aatttagcat cgatttctgc caattcagca tccaaggctt taacctcagc agatagattt
     2641 tgcatggcag caatcttgtc atctgtattt tccttgttgc gcttagcttg ggcaatctca
     2701 gcagaaactg tgttacgttc tgctttgaga gtttcaacct tgaccaagat gttacgacgt
     2761 ttagcatcga tttctttcat ttcattcaag acagcagcat ctacaccacg tgtagctaat
     2821 ttttctgcga cagcttcaaa atctgtacga atacgtttga tatctaacat aagaactcct
     2881 ttatgaaaaa agcacgcctg acaaaacgtt ggagtggcag ggccacggtt ccatccaact
     2941 tcacaggtgt gcacttgatt gtgtatgtaa ttgttactaa cggtagaatt tcacctatcc
     3001 ctcctatctg ctcgcagtac ccgcagactt tctgaaagaa gaagataacc tacttatccg
     3061 ttgctatgat tatactaaag tttctacttt tttgcaaata gatttttaaa tttttggcta
     3121 attgtctgaa tcagggtcgg aagtttgacg accttgtcat tgcctagttt tttgcgtgca
     3181 attttgagaa tggcacctga gtcttttgaa gcaaagagga attttccttt gtctgtaaag
     3241 acttcgaagt ggcggctgat tttgcgtcca gtgacattgg ctccaatctg attgatatgg
     3301 ctccaaggaa tctggataaa ttgttcgaca ttgacatctg ggtaaaattc caaagcctga
     3361 tctccgataa ggaatttccc aactttccca gcgatagaga ggtaggaagt gcctgtcgta
     3421 ctgaggagta ctgttttgtt aagtgattga gccatgctta gtcttcctta ctttctccaa
     3481 aaaaggcatt gtagagggct ttaattgctg ctttctcttg gtctttattg acaacaaaca
     3541 taatagaaac ttcactagaa ccttgagaca tcatctggat gttgattttg ttttcagata
     3601 gagcgcgtgt cgcagtagca gtcactccga tatggctctt cattttttca ccaacaatca
     3661 taatgataga aaggtcgtgt tcgatttctg catgatctac tttagccttt tgaaccaact
     3721 gacgcaggat ttcttcttcc ttgatgggag ttagttggcg agaacggaga atgatagaaa
     3781 gatcgtcgat acctgttggc atatgttccc aaccgatgtt aagttcttcc aggatttgca
     3841 gaaccttgcg gccaaatcca acctcacggt tcatgaggta tttcgacatg ttaatgctga
     3901 caaagcctga gtcaccagca attcccacaa ctggaaattc atcattactg tgttttagaa
     3961 cgatacgagt acctggatgg tcagggttgt tggtattctt gataaccaga ggaatttttc
     4021 cacggtaggc aggaagaaga gcctcgtcat gaaggactga gaagcctgca taggccaact
     4081 cgcgcatttc acggtaggtc aactcaggaa tcgagtgtgg ttggtggata ataccagggt
     4141 gggctgcaaa gataccatca acgtccgtaa agttttcata gaggtcagct ttgacaccag
     4201 cagcaatgat agaacctgta atatcagaac ctccacgtga gaaagtacag atttgatttt
     4261 ccttagtgac accaaagaaa ccaggaatga caaggacttc attggtgttt gtcaattctt
     4321 caatcttgtc ataacttgat ggaatgatgc gagcgtgacc aggttcactt gtgaccacaa
     4381 tcccagcttc tctagggtgc atatagcgtg catcgatacc attttggtta aagtaggcag
     4441 caatcaattt ggcattgtta ttttcaccgg ctgctaggaa agtatcgtag agaaattcat
     4501 tttcttcagt aggaagagtg gccaaggcgt gaatgctttt agaaattttt tctagcacag
     4561 ctggttttag tcccaattca ctaaccatag cagcatagcg gtcgataatc cagctttggc
     4621 tcttgctaat atcgttaccc gcaacatagt cgcggtagta tttaatcagg gcatccgtaa
     4681 ccttagtatc ttcagcattg cgtttaccag gcgcagaaac gactacaaaa cgacgctctg
     4741 aatcgctttt gacgatgttt aaaacttttt ctaattgact agcagaggca agagaactac
     4801 ctccaaattt aacaactttc ataagaactc ctaaagtaag tattttatac gattatagca
     4861 gaaagaaagg cttttttcaa tgaagagaat aagatgcttt ctagcctaaa gcgagctttt
     4921 cttatcggct gagacacttt cagtcgattt tttcttgctt tctgatccta aaaaagatat
     4981 actttgacag tgaaataatt taacactcaa tgaaaatcaa agagcaaact aggaagctag
     5041 acgaagtcag ctcaaaatac tgttttgagg ttgcagatgg aagctgacgc ggtttgaaga
     5101 gattttcgaa gagtataaac tgcttataaa ataaaaagga gccctgatgg aacacattat
     5161 ttatcaactt gaagaggact tggcaatcct taccttgaac cgtcctgagg tcgcaaatgg
     5221 ttttcatatt cccatgtgtg aggagatttt agaagctctg actttggcag aagaaaatcc
     5281 agctgtgcat tttatcttaa tcaatgccaa tggaaaggtc ttctcagttg ggggagattt
     5341 ggtagagatg aagcgagcag tggatgagga tgatattcca tcattgacaa aaatcgcaga
     5401 gttggtcaat acgatttctt ataaaatcaa gcaaatagcc aaacctgttt taatggaagt
     5461 tgacggtgct gttgcaggtg ccgcagcgaa tatggctgtt gcggcagatt tctgtttggc
     5521 gacggataag gctaagttta tccaagcctt tgttggtgtt ggtttggctc cagatgcagg
     5581 gggaattcat ctcttgagcc gcagtatcgg tgtgacgcgt gctgctcaat tagctatgac
     5641 aggagaggct ttaacggcag aaaaagccct agaatggggg ttggtttacc gcgtttctga
     5701 agctgaaaaa cttgaaaaga cgagagaaca gcttcttaaa aaattaagac gtgcttcaag
     5761 taattcctat gctgccatta agaagttggt ttgggagagc cagtttaaag actggcaggg
     5821 ttacgctact ttagaactga acctacagaa atccttagct caaacagagg atttcaaaga
     5881 aggagtgcga gctcattcgg agagaagaag acctaaattt acaggaaaat aaaaaatact
     5941 tgcatcattc tttgaatttt gatataattc tttctgtcaa atgttttgat tgtaaaagtt
     6001 ttttgaagaa ggagggatat gaaaaatgga ctaccaacga atcaatgaat atttaacatc
     6061 tatatttaac aatgtccttg taattgagga agtgaacttg agaggtagtc gttttaagga
     6121 tatctccatc aaagaaatgc atacgattga tgtcatcggt aaggctccag atgtgactcc
     6181 aagtcaagtg tcaaaagagt tgatggtaac tcttggaact gttacgacaa gtttgaacaa
     6241 tttagagcgt aagggctaca ttgagcgagt tcggtcagaa caggatcgtc gtgtggtgca
     6301 tctgcatttg acaaagaagg gtcgcttgat tcatagactg cataaacgct tccacaaggc
     6361 catggtagaa aaaattattg atggcatgag cgaggaagaa attgctgtca tgggtaaagg
     6421 cttgactaat ctttaccaat ttttggagga tttgaaataa tggcttttgc aaaaataagt
     6481 caggttgctc attatgtgcc agagcaagtg gttacaaatc acgacttggc tcagattatg
     6541 gataccaatg atgagtggat ttcaagtcga acgggaatac gacaaaggca tatttcaaga
     6601 acagaatcta ccagtgattt ggctacagag gttgctaaga aactgatggc aaaagctgga
     6661 ataacaggaa aagaactgga ttttatcatc ctagctacca ttactccaga ttcgatgatg
     6721 ccctctacag ctgctcgtgt tcaagctaat attggcgcta ataaagcctt tgcttttgac
     6781 ttaaccgcag cttgcagtgg atttgtattt gctctttcaa ctgctgaaaa gtttatcgct
     6841 tctggtcgct ttcaaaaagg cttggtgatt ggtagtgaaa ccctctctaa ggcagtcgat
     6901 tggtcggatc gatcaacagc tgtgttgttt ggagatggtg ctggtggtgt cttgttagaa
     6961 gctagcgagc aagagcattt cttagctgag agtctcaata gcgatggaag tcgcagcgag
     7021 tgtttaactt atgggcattc aggtttgcat tctccatttt cagatcaaga aagtgcagat
     7081 tcgtttttga agatggatgg acgcacagtc tttgattttg ccattcgaga tgtagccaag
     7141 tctatcaagc agactattga tgaatctcct atagaggtga cagacttgga ttatctgcta
     7201 cttcatcaag ccaatgaccg tattttggat aagatggcta gaaaaattgg tgttgaccga
     7261 gccaaacttc cagccaatat gatggaatat ggcaatacca gtgcagccag tatcccgatt
     7321 ttactttcag agtgtgtaga acaaggtctc atccctttag atggtagcca gactgttctt
     7381 ctatcaggct tcggtggagg cttgacctgg ggcacgctca ttcttacaat ttaggtaatc
     7441 atgtggtgaa cacattgtta taaaaaaact atttatttta aaggagtcct atcatggcag
     7501 tatttgaaaa agtacaagaa attatcgttg aagaacttgg aaaagacgca tcagaagtaa
     7561 cacttgaatc aacttttgat gatttggacg cagattcatt ggacttgttc caagtaatct
     7621 cagaaatcga agatgctttt gatatccaaa tcgaagcaga aaatgacttg aaaacagttg
     7681 gtgacttggt tgcttacgtt gaagagcaag caaaataagc agaatattag tagaaggagt
     7741 agggagaaac ccactccctc ttgtttaggt aatagtttga ctgtcaaatt atggtgaaat
     7801 cgaactatta cgtaagcaag gaaggttgga ggcactatga aaacgcgtat tacagaatta
     7861 ttgaagattg actatcctat tttccaagga gggatggcct gggttgctga tggtgatttg
     7921 gcaggggctg tttccaaggc tggaggatta ggaattatcg gtgggggaaa tgccccgaaa
     7981 gaagttgtca aggccaatat tgataaaatc aaatcattga ctgataaacc ctttggggtc
     8041 aacatcatgc tcttatctcc ctttgtggaa gacatcgtgg atctcgttat tgaagaaggt
     8101 gttaaagttg tcacaacagg agcaggaaat ccaagcaagt atatggaacg tttccatgaa
     8161 gctgggataa tcgttattcc tgttgttcct agtgtcgctt tagctaaacg catggaaaaa
     8221 atcggtgcag acgctgttat tgcggaagga atggaagctg gggggcatat cggtaaatta
     8281 acaaccatga ccttggtgcg acaggtagcc acagctatat ctattcctgt tattgctgca
     8341 ggaggaattg cggatggtga aggtgctgcg gctggcttta tgctaggtgc agaggctgta
     8401 caggtgggga cacggtttgt agttgcaaaa gagtcgaatg ctcatccaaa ctacaaggag
     8461 aaaattttaa aagcaaggga tattgatact acgatttcag ctcagcactt tggtcatgct
     8521 gttcgtgcta ttaaaaatca gttgactaga gattttgaac tggctgaaaa agatgccttt
     8581 aagcaggaag atcctgattt agaaatcttt gaacaaatgg gagcaggtgc cctagccaaa
     8641 gcagttgttc acggtgatgt ggatggtggc tctgtcatgg caggtcaaat cgcagggctt
     8701 gtttctaaag aagaaacagc tgaagaaatc ctaaaagatt tgtattacgg agccgctaag
     8761 aaaattcaag aagaagcctc tcgctgggca ggagttgtaa gaaatgacta aaacagcctt
     8821 tttatttgct ggtcaaggtg cccagtatct agggatggga cgggatttct atgatcagta
     8881 tccggttgtc aaagaaacga ttgatcgagc gagtcaggtg ctcggttatg atttacgtta
     8941 tctcatcgat acggaagaag acaaactcaa tcagacccgc tatacgcaac cagccattct
     9001 agcgacttcg gttgctatct accgtttatt gcaagaaaag ggctatcagc ctgatatggt
     9061 tgctggtttg tctcttggag aatactctgc cttggtggca agcggcgcct tggattttga
     9121 agatgcggtt gccttggtag ctaagcgtgg agcctatatg gaagaagcgg ctcctgctga
     9181 ctctggcaag atggtagcag ttctcaatac gccagtagag gtcattgaag aagcctgtca
     9241 aaaagcttct gaacttggag tggttactcc agccaactat aacacacctg cacaaatcgt
     9301 cattgctgga gaagtggttg cagttgatcg agcggttgaa cttttgcaag aagcaggtgc
     9361 caaacgcttg attcctctta aggtgtcagg tccctttcac accgctctcc ttgaacctgc
     9421 tagccagaaa ctagctgaaa ctctagctca ggtaagtttt tcagatttta cttgtcccct
     9481 agtcggcaat acagaagcta ctgtgatgca aaaagaggac attgctcagc tcttgacgcg
     9541 tcaggtcaag gaacccgttc gtttctatga aagtattggg gtcatgcaag aagcaggcat
     9601 aagcaacttt atcgagattg gaccggggaa agtcttgtca ggttttgtta aaaaaattga
     9661 tcaaactgct cacttagctc atgtggaaga tcaagcgagt ttagtagcac ttttagaaaa
     9721 atagactaaa ataagtagaa gttttgaaag gaaaaaaatg aaactagaac ataaaaatat
     9781 ctttattaca ggttcgagtc gtggaattgg tcttgccatc gcccacaagt ttgctcaagc
     9841 aggagccaac attgtcttaa acagtcgtgg ggcaatctca gaagaattgc tcgctgagtt
     9901 ttcaaactat ggtatcaagg tggttcccat ttcaggagat gtatcagatt ttgcagacgc
     9961 taagcgtatg attgatcaag ctattgcaga actgggttca gtagatgttt tggtcaacaa
    10021 tgcagggatt acccaagata ctcttatgct caagatgaca gaagcagatt ttgaaaaagt
    10081 gctcaaggtc aatctgactg gtgcctttaa tatgacacaa tcagtcttga aaccgatgat
    10141 gaaagccaga gaaggtgcta tcattaatat gtctagtgtt gttggtttga tggggaatat
    10201 tggtcaagct aactatgctg cttctaaggc tggcttgatt ggctttacca agtctgtggc
    10261 acgcgaggtc gctagtcgga atatacgagt caatgtgatt gctccaggaa tgattgagtc
    10321 tgatatgaca gctatcttat cagataagat taaggaagct acactagctc agattccgat
    10381 gaaagaattt gggcaggcag agcaggttgc agatttgaca gtatttttag caggccaaga
    10441 ttatctaact ggtcaagtgg ttgccattga tggtggctta agtatgtagc gaaagctaga
    10501 ggtgaaaaga atgaaactaa atcgagtagt ggtaacaggt tatggagtaa catctccaat
    10561 tggaaataca ccagaagaat tttggaatag tttagcaact gggaaaatcg gcattggtgg
    10621 cattacaaaa tttgatcata gtgactttga tgtgcataat gcggcagaaa tccaagattt
    10681 tccgttcgat aaatacttta taaaaaaaga taccaaccgt tttgataact attctttata
    10741 tgccttgtat gcagcccaag aggctgtaaa tcatgccaat cttgatgtag aggctcttaa
    10801 tagggatcgt tttggtgtta tcgttgcatc tggtattggt ggaatcaagg aaattgaaga
    10861 tcaggtactt cgccttcatg aaaaaggacc caaacgtgtc aaaccaatga ctcttccaaa
    10921 agctttacca aatatggctt ctgggaatgt agccatgcgt tttggtgcaa acggtgtttg
    10981 taaatctatc aatactgcct gctcttcatc aaatgatgcg attggggatg ccttccgctc
    11041 cattaagttt ggtttccaag atgtgatgtt ggtgggagga acagaagctt ctatcacacc
    11101 ttttgccatc gctggtttcc aagccttaac agctctctct actacagagg atccaactcg
    11161 tgcttcgatc ccatttgata aggatcgcaa tgggtttgtt atgggtgaag gttcagggat
    11221 gttggttcta gaaagccttg aacacgctga aaaacgtgga gctactatcc tggctgaagt
    11281 ggttggttac ggaaatactt gtgatgccta ccacatgact tctccacatc cagaaggtca
    11341 gggagctgtc aaggccatca aactagcctt ggaagaagct gagatttctc cagagcaagt
    11401 agcctatgtc aatgctcacg gaacgtcaac tcctgccaat gaaaaaggag aaagtggagc
    11461 tatcgtagct gttcttggta aggaagtacc tgtatcatca accaagtctt ttacaggaca
    11521 tttgctgggg gctgcgggtg cagtagaagc tatcgtcacc atcgaagcta tgcgtcataa
    11581 ctttgtacca atgacagctg ggacaagtga agtatcagat tatatcgaag ctaatgtcgt
    11641 ttatggacaa ggcttggaga aagaaattcc atacgctatt tcaaatactt ttggttttgg
    11701 aggccacaat gcagttcttg ctttcaaacg ttgggagaat agataagtat gaatttaaac
    11761 gatattaaag acttgatgac tcaatttgac cagtcaagtt tgagagaatt ttcttataaa
    11821 aatgggacgg atgagttgca gtttagcaag aatgaagcga gacctgtgcc tgaagttgca
    11881 actcaagtcg ctccagcacc cgttctagca acaccgagtc cagtagctcc tacatctgct
    11941 ccagcagaga ctgtagcaga agaagttcca gctccagctg aagcaagtgt ggctactgag
    12001 ggaaatcttg tagagagtcc acttgttgga gtggtttact tggctgctgg accagataaa
    12061 cctgccttcg ttacagttgg tgatagtgtc aaaaaaggtc aaacattggt aattatcgaa
    12121 gccatgaaag tcatgaatga aatcccagct cctaaggatg gtgtggtaac ggaaattctc
    12181 gtctctaacg aagaaatggt tgagtttggt aaaggattgg tacgtatcaa atgatcgata
    12241 ttcaaggaat caaagaagcc cttccccacc gttatcctat gcttctagtg gaccgtgtct
    12301 tggaagtgag cgaggatacc attgttgcta tcaaaaatgt gaccatcaac gagcctttct
    12361 ttaacggcca ctttcctcaa tacccagtta tgccaggtgt tctgattatg gaagccttgg
    12421 cgcaaactgc cggtgtgttg gagttatcaa aacctgaaaa taaaggaaaa ctggtctttt
    12481 acgctggtat ggacaaggtt aagtttaaga agcaagttgt accaggcgac caattggtta
    12541 tgacagcgac ttttgtaaaa cgtcgtggca ccatagctgt ggttgaagca aaggctgaag
    12601 tggatggcaa gcttgcagcc agtggtaccc ttacttttgc aattgggaac taaggaggtt
    12661 ctccatgttt cgaaaaattt taattgccaa tcgtggtgaa attgcggttc gtattatccg
    12721 tgcggcacgt gaattgggga ttgcgacggt agcggtttat tcaactgctg ataaggaagc
    12781 tcttcatacg cttttggcag atgaagcagt ttgtattggt cctggcaagg caacagagtc
    12841 ttatctcaat attaatgcag ttctatcagc tgcagtcttg actgaggcag aagctattca
    12901 ccctggtttt ggatttctca gtgaaaattc caaatttgcg accatgtgtg aagaagtagg
    12961 tatcaagttt atcggtccat ctggtcatgt tatggatatg atgggggata agatcaatgc
    13021 gcgtgctcag atgattaaag caggtgtgcc tgttatacca ggttcagatg gagaagtgca
    13081 taactctgaa gaagctttga ttgttgctga aaaaattggc tatcctgtta tgctcaaggc
    13141 ttcagcaggt ggaggtggta aagggattcg taaggttgaa aaaccagatg acctcgtttc
    13201 tgcctttgaa actgcctcta gtgaggccaa ggccaattat ggcaatggtg ccatgtacat
    13261 agaacgggtt atctatccag ctcggcacat tgaggttcaa atcctaggtg atgagcatgg
    13321 acatgtgatt cacttgggtg aacgggattg ttctcttcaa aggaataacc aaaaggtttt
    13381 ggaagaaagt ccctcgattg caatcggaaa aacgctgcgt catgaaatag gtgctgctgc
    13441 tgttcgagcg gcagagtttg ttggctatga gaatgcagga accattgaat ttcttcttga
    13501 tgaagcaagt agcaatttct atttcatgga gatgaatact cgtgttcagg tagaacatcc
    13561 agtaacagag tttgtttcag gtgttgatat cgttaaggaa cagatttgca ttgcggcagg
    13621 tcagcctttg tctgttaagc aagaagatat tgtcctacgt ggtcatgcca tcgagtgtcg
    13681 tatcaatgca gaaaacccag cctttaactt tgctccaagt ccaggtaaga ttactaatct
    13741 ctatctgcca agtggtggag ttggcttgcg cgtggattca gcagtttatc caggttatac
    13801 cattccgcct tattatgata gtatgattgc caaaatcata gtacacggcg aaaatcgttt
    13861 tgacgccttg atgaaaatgc aacgtgccct ctatgaatta gagattgaag gagtgcagac
    13921 taatgcagat ttccagcttg acctcatttc agatcgcaat gtcattgctg gggattatga
    13981 tacttccttc ttgatggaaa ccttcttacc taaatatcaa gaaaaagaat aaaatgactt
    14041 taagagttta aaccgaaaag gggaatcaat ggctctattt agtaaaaaag ataagtatat
    14101 tcgaatcaat cccaatcgtt cggttaggga aaaacctcaa gctaagccag aggttccaga
    14161 tgaattattt tcccagtgtc caggctgtaa gcataccatc tatcagaagg atctgggaag
    14221 tgaacgtatc tgtccgcact gtagctatac ctttcgtatt tctgcccaag aacgcttggc
    14281 tttgacgatt gatatgggaa ccttcaaaga attgtttaca gggattgaaa gcaaggatcc
    14341 cttgcatttc cctggttacc aaaagaaact ggcatctatg cgtgaaaaaa caggtctgca
    14401 tgaagccgtt gtgacaggaa ctgctcttat taaaggtcag actgtggctc ttgggattat
    14461 ggattctaac tttatcatgg cttctatggg tacggttgta ggtgaaaaaa tcactcgttt
    14521 gtttgagtat gcgactgtcg aaaaattgcc agttgtccta ttcacagcct ctggtggagc
    14581 ccgtatgcag gaaggaatca tgagtctcat gcagatggct aagatctctg cggcggttaa
    14641 acgccattca aatgctggtc tcttttacct gaccattttg acagatccaa cgactggtgg
    14701 tgtgacagca tctttcgcta tggaaggcga tatcattctg gctgaaccac agagcttggt
    14761 tggttttgct gggcgtcgtg tgattgaaaa tacggttcgt gaaagcttgc ctgaggattt
    14821 ccaaaaggca gaattcctat tagaacatgg ctttgtggat gctattgtca aaagaagaga
    14881 cttaccagat acgattgcta gcctagtcag attgcatgga gggagtccta gatgaatatt
    14941 gcaaaaatag tcagagaagc gcgtgagcag agtcgcttga caaccttgga ctttgcgaca
    15001 ggcatttttg atgaatttat ccaattacat ggtgaccgtt cttttcgtga tgatggtgca
    15061 gttgttggtg gtattggttg gcttggagac caagctgtaa cagtggttgg tatccaaaaa
    15121 ggcaagagtt tgcaagacaa cctcaaacgg aattttggcc aaccacatcc agaaggctac
    15181 cgaaaggcac tgcggttgat gaaacaggct gagaaatttg gccgtccagt tgtgaccttt
    15241 atcaacacag caggtgctta tcctggtgtc ggagcggaag aacgtggtca aggggaagct
    15301 atcgctcgca atctcatgga aatgagtgac ctgaaagttc ctattatcgc cattattatc
    15361 ggtgaaggtg gttcaggcgg ggctctggct ctagctgtcg cggaccgtgt ctggatgctg
    15421 gaaaattcta tctatgccat tctcagtcca gaaggctttg cttccatttt atggaaggac
    15481 ggtactcgcg ccatggaagc agcagaactg atgaaaatca cttcgcatga actgttagaa
    15541 atggacgtgg tggataaggt gatttctgaa gtaggacttt ctagtaaaga actgattaag
    15601 agtgtcaaaa aagaactcca aacggagctg gctagacttt cacaaaaacc gctagaagag
    15661 ttgctggaag aacgctatca acgatttaga aaatactaag aatgactaaa tgagactaga
    15721 aaggtctcat tttttatgga aaaatgcaat tcaggttgaa aaattgacag ttgaggagat
    15781 gggctataaa atagacaaaa aagatgtata ctgtagatgt aagcttacaa aaat
//
LOCUS       DAVGJL010000038        15571 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.038, whole genome shotgun sequence.
ACCESSION   DAVGJL010000038 DAVGJL010000000
VERSION     DAVGJL010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15571
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.038"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            82..486
                     /locus_tag="VVE42_000972"
     CDS             82..486
                     /locus_tag="VVE42_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073182.1"
                     /translation="MKILKRYILELCFILSFALPFIKGANADNGRCFVETYYGFTFLM
                     EHAIVTAVFICSFLIAFLLKKRWAKWIAAGSYCFLVLWIATEGYFFRMSLEDLIRLWT
                     SLEILTQTYQLGFYLNILLGILLIIKYFKVKQ"
     gene            702..1283
                     /locus_tag="VVE42_000973"
     CDS             702..1283
                     /locus_tag="VVE42_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU3073183.1"
                     /translation="MYYAENPDAAHDIHELRVELLGQKMTFLTDAGVFSKKIVDFGSQ
                     LLLKCLEVNQGETVLDVGCGYGPLGLSLAKAYGVQATMVDINTRALDLARRNAEKNNA
                     KATIFQSNIYEQVEGHFDHVISNPPIRAGKQVVHEIIEKSKDFLETGGDLTIVIQKKQ
                     GAPSAKSKMEDVFGNCEILKKDKGYYILRSVKE"
     gene            1280..2557
                     /locus_tag="VVE42_000974"
     CDS             1280..2557
                     /locus_tag="VVE42_000974"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEU3073184.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLVEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSDIDGVKVDKHSTGGVGDKVTLILAPLVASF
                     GVPVAKMSGRGLGHTGGTIDKLESIKGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELA
                     QTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEILQGQGRQDITHFICELA
                     QIMLGLANVNKTVEEVRQHLENGQALAKFEEMVQAQGGDLEDLYRPVNVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDALDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
     gene            2575..3237
                     /gene="deoC"
                     /locus_tag="VVE42_000975"
     CDS             2575..3237
                     /gene="deoC"
                     /locus_tag="VVE42_000975"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEU3073185.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYDFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSAVKAFETKEAIQNGADEIDMVINVGALKSGNL
                     ALVESDIRAVVEASGDKLVKVIIEACLLTDQEKIVVCQLAQKAGADFVKTSTGFSTGG
                     ATIADVTLMRETVGSDMGVKAAGGARSYADALAFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            3224..3613
                     /locus_tag="VVE42_000976"
     CDS             3224..3613
                     /locus_tag="VVE42_000976"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEU3073186.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGSVYTGVNIE
                     NASYPLTNCGERTAIFKAISEGQREFSELIVYGQTEKPISPCGACRQVMAEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            3703..4755
                     /locus_tag="VVE42_000977"
     CDS             3703..4755
                     /locus_tag="VVE42_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEU3073187.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQAWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALHNAVKDAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDISNKAEKGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            4899..6434
                     /locus_tag="VVE42_000978"
     CDS             4899..6434
                     /locus_tag="VVE42_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3073188.1"
                     /translation="MAHENVIEMRDITKVFGGFVANDKINLHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVADISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIRRG
                     KSIETVEIAGATNADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQELGVLMAGGNLGKEK
                     SDV"
     gene            6427..7485
                     /locus_tag="VVE42_000979"
     CDS             6427..7485
                     /locus_tag="VVE42_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU3073189.1"
                     /translation="MFKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILATIVIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KDFMQSTDSTIRVGANATYQTPWLAELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGAVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            7488..8444
                     /locus_tag="VVE42_000980"
     CDS             7488..8444
                     /locus_tag="VVE42_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU3073190.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPVIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGVIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
     gene            8529..9876
                     /locus_tag="VVE42_000981"
                     /pseudo
     CDS             8529..9876
                     /locus_tag="VVE42_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(9919..10560)
                     /gene="plsY"
                     /locus_tag="VVE42_000982"
     CDS             complement(9919..10560)
                     /gene="plsY"
                     /locus_tag="VVE42_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEU3073191.1"
                     /translation="MITIVLLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILSNYDSLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
     gene            10696..12639
                     /gene="parE"
                     /locus_tag="VVE42_000983"
     CDS             10696..12639
                     /gene="parE"
                     /locus_tag="VVE42_000983"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEU3073192.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KIGTALKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLTDKRTDE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGLKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLAAVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVVNTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEEATVF"
     gene            13059..15530
                     /gene="parC"
                     /locus_tag="VVE42_000984"
     CDS             13059..15530
                     /gene="parC"
                     /locus_tag="VVE42_000984"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEU3073193.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKKTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDVAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQVGIVPILSSYIAHRREVILARSRFDKEKAEKRLHIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLA
                     AIIGDERTMYNLMKKELREVKKKFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEEILYVEVLDQFDDATTYFAVTRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVVTEYK"
BASE COUNT         4668 a         2847 c         3608 g         4448 t
ORIGIN      
        1 tgatgaaata ggagatagat tctactgatt tataagctta taaagggggc tttttagttc
       61 acaacttata ggaggtgtac tatgaaaatc ttaaaacgtt acatattgga actctgtttt
      121 attttaagtt ttgctttacc ttttataaaa ggagccaatg cagataatgg tagatgcttt
      181 gtggaaacct attacggttt tacttttttg atggaacatg ctattgtaac agctgtcttt
      241 atctgttcgt ttttaattgc tttcttacta aaaaaacgat gggcgaaatg gattgctgct
      301 ggtagttatt gctttttagt cctatggatt gcgacagagg gttatttctt ccgcatgtca
      361 ctagaagatt tgatacgact ttggacaagt ttggaaatcc tgacacaaac gtatcagttg
      421 ggcttttatc taaacatttt gttgggaatt ttgttgataa taaagtattt taaggttaag
      481 caatagaaat aataatctgc ctgattatag atgataaact ttttgttttt atctggactg
      541 tgatatttat agttctaaat ggtttattat ttataagtgt tgaaagagat tgaagaaaat
      601 ggcttcgtga ctcgctttct tcggtctttt tcgttttatt atcaatttgt aaacgattta
      661 caatttgagt caaattatgg taaaatagtg tcatgagtaa aatgtattat gcagaaaatc
      721 ctgacgctgc tcacgacatt catgagttga gagtagagtt gttgggacaa aaaatgacct
      781 ttttaacgga tgcgggtgtt tttagcaaga aaatagttga ctttggaagt caactattgc
      841 tcaagtgtct tgaggtcaac caaggagaaa ctgtccttga tgtaggctgt ggttacggac
      901 ctttgggttt gtccttagct aaggcttatg gagttcaggc tactatggtt gatatcaaca
      961 ctcgtgcctt ggatttagct cggagaaatg ctgaaaaaaa taatgcaaaa gcgacgattt
     1021 tccagtccaa catctatgaa caagttgaag gacattttga tcatgtcatt tccaatcctc
     1081 ctatccgtgc gggcaagcaa gtcgttcatg aaatcattga gaaaagtaaa gatttcttgg
     1141 aaactggtgg ggatttgaca atcgtcatcc agaaaaaaca aggggctcca agtgctaagt
     1201 ccaagatgga ggatgtgttt ggtaattgtg aaatccttaa aaaagacaaa ggatactata
     1261 tccttagaag tgtgaaagaa tgagagcagt tgatttaata caaaaaaaac gagacggtca
     1321 agaactgact tcaagtgaaa ttgaatggct ggtagaaggc tatgtgtcag gaactgttcc
     1381 tgattatcag atgtctgcct ttgctatggc tgtttatttt aaaggaatga cgactcgtga
     1441 aatttctgat ttaacgatga atatggttaa gactgggcaa gagtttgacc tgtcagacat
     1501 tgatggtgtg aaagttgaca agcattctac tggtggtgtc ggcgataagg taaccttgat
     1561 tttagctcct cttgttgcta gcttcggtgt gcctgtagca aaaatgagtg gtcgtggtct
     1621 tggccataca ggcggaacaa ttgataaatt ggagtccatt aagggctatc aagtagaacg
     1681 cagtcaagag gattttattc gtcaggttca ggatattgga gtatctgtca ttgggcaatc
     1741 agaccagctg gtcaaagcag ataagcttct ctatgccctt cgtgatgtga ccgcaactgt
     1801 cgacacgatt cctttgattg cgagttcggt gatgagcaag aaaattgcgg caggggcgga
     1861 tgctattttg ctagacgtga ctgtcggtga gggtgccttc atgaagacgg ttgatgaggc
     1921 gcgtgagttg gctcaaacta tggtagatct tggtaaggtt gttggacgga agacagtagc
     1981 agtgattacg gatatgagcc agcctttggg acgagcgatt ggaaatcgtc tggaaatcct
     2041 tgaagcattg gagattttgc aaggtcaagg acgtcaggat attacccact ttatttgtga
     2101 attggctcaa attatgcttg gtttggcaaa tgttaacaaa actgttgaag aagttcgcca
     2161 acatcttgag aatggtcaag cactggctaa gtttgaggaa atggtgcaag cccaaggtgg
     2221 agacttggaa gacctctatc gtcctgtaaa tgtagctcat gtggtggaaa tccctgctca
     2281 ggaaacgggt gtcatttcag ctcttccagc tatggacttt ggtctttatg ccatgaggct
     2341 gggtgctggt cgtgcagtta agtctgatgc cttggactat gaaacaggaa ttgtttttga
     2401 aaagaagatt ggagactccg ttcaaaaggg tgaaattgtt gcaaaagtat acacaaatga
     2461 aaaaattcca cctcaactag ttacagattt ccaaaaatgt gttaaaataa gtgatgaagt
     2521 gaaaaaaatc cgtgaaattg tagaaatcat ctcctaaatg gagggaaaac cgaaatgaaa
     2581 ttaaataaat atatagatca tacgctttta aaacaagatg caaagaaaaa acaaattgat
     2641 agtttgttgt ctgaggctag agagtatgac tttgccagtg tttgcgttaa tccgacctgg
     2701 gttgaacatg ctaaaaaagg acttgaaggc acagatgtta aggtttgcac agtagtaggt
     2761 ttccctttgg gagcaacaac ttcagccgtg aaagcatttg agacaaaaga agctatccaa
     2821 aatggtgcag atgagattga tatggtaatc aatgttggag ctctcaaatc aggtaattta
     2881 gccttggttg agtcagatat tcgcgcagta gtggaagcaa gtggtgataa gttagtgaaa
     2941 gtcattattg aagcttgcct tctgacagac caagaaaaaa ttgttgtttg ccaattggcc
     3001 caaaaagctg gggctgactt tgttaaaaca tctactggtt tttcaactgg tggtgctacg
     3061 atagcagatg ttacattaat gcgtgaaaca gttggatctg atatgggagt caaggctgct
     3121 ggtggagctc gttcttatgc agatgctctt gcctttgtcg aagcaggtgc gacccgtatc
     3181 ggaacgtcag ctggggtagc tattttaaaa ggagaattgg cagatggcga ctactgagtt
     3241 gattgaactg gcaattgaaa ccagcaaaca tgcatatgtc ccctattctc actttcctat
     3301 cggagcagtt ttagtagcca aagacggaag tgtttacacg ggagtgaata tcgaaaatgc
     3361 tagctatcct ttgaccaatt gcggtgagag aacagctatt tttaaggcta tatctgaagg
     3421 ccaaagagaa ttttcagaat tgattgtcta tggtcaaact gaaaaaccga tctcgccatg
     3481 tggtgcttgt cgccaagtga tggctgaatt ttttgaacaa gatctaaaag tgaccttagt
     3541 cgcaaaagat aaatcgacgg tcgagatgac ggtcggggaa ttacttccat actcttttac
     3601 agacttaaac tagtctgagt cgctctctga gtggcacggt ccttgtggcc aaccaatcca
     3661 tacttgcaac atcgttgcac atcttattta ggaggttcag taatgaacaa gaaacaatgg
     3721 ctaggtcttg gcctagttgc agtggcagca gttggacttg ctgcatgtgg taaccgctct
     3781 tctcgtaacg cagcttcatc ttctgatgtg aagacaaaag cagcaatcgt cactgatact
     3841 ggtggtgttg atgacaaatc attcaaccaa tcagcttggg aaggtttgca ggcttggggt
     3901 aaagaacaca atctttcaaa agataacggt ttcacttact tccaatcaac aagtgaagct
     3961 gactacgcta acaacttgca acaagcggct ggaagttaca acctaatctt cggtgttggt
     4021 tttgcccttc ataatgcagt taaagatgca gcaaaagaac acactgactt gaactatgtc
     4081 ttgattgatg atgtgattaa agaccaaaag aatgttgcga gcgtaacttt cgctgataat
     4141 gagtcaggtt accttgcagg tgtggctgca gcaaaaacaa ctaagacaaa acaagttggt
     4201 tttgtaggtg gtatcgaatc tgaagttatc tctcgttttg aagcaggatt caaggctggt
     4261 gttgcgtcag tagacccatc tatcaaagtc caagttgact acgctggttc atttggtgat
     4321 gcggctaaag gtaaaacaat tgcagccgca caatacgcag ccggtgcaga tattgtttac
     4381 caagtagctg gtggtacagg tgcaggtgtc tttgcagagg caaaatctct caacgaaagc
     4441 cgtcctgaaa atgaaaaagt ttgggttatc ggtgttgatc gtgaccaaga agcagaaggt
     4501 aaatacactt ctaaagatgg caaagaatca aactttgttc ttgtatctac tttgaaacaa
     4561 gttggtacaa ctgtaaaaga tatttctaac aaggcagaaa aaggagaatt ccctggcggt
     4621 caagtgatcg tttactcatt gaaggataaa ggggttgact tggcagtaac aaacctttca
     4681 gaagaaggta aaaaagctgt cgaagatgca aaagctaaaa tccttgatgg aagcgtaaaa
     4741 gttcctgaaa aataatggat ggaagtcatt tcttaggaag cggccaaggg ccgctttttt
     4801 aagagttgag acaaagtcat tttgtctcag taaagcttgc tactagacaa actagcaagt
     4861 tttattgaaa taaaataaga ctctgaaagg aagagcacat ggcacacgaa aatgtcattg
     4921 agatgcgtga tattaccaag gtgtttggtg gatttgttgc caacgacaaa atcaacttgc
     4981 acctacgaaa aggtgaaatt catgcacttt taggagaaaa tggggctggt aagtccacgc
     5041 taatgaacat gttagcaggc cttcttgaac caactagtgg tgaaatcgcg gtcaacggtc
     5101 aagttgtcaa tctcgactcc ccatctaaag cagctagctt gggaatcggg atggttcacc
     5161 agcactttat gttggttgaa gccttcacag tggctgaaaa catcatttta ggtagtgaat
     5221 tgactaaaaa tggtgtgcta gatatcgctg gagctagcaa agaaatcaag gctctttctg
     5281 aacgttatgg cttagctgtt gacccttctg ccaaggtagc agatatctca gttggagccc
     5341 aacaacgtgt agaaatttta aaaacacttt atcggggggc tgatatcctt atctttgacg
     5401 aaccaacggc tgttttgact ccatcagaaa ttgatgagtt gatggctatt atgaaaaatc
     5461 ttgtcaaaga aggaaaatca attatcttga ttacccacaa attggatgaa attcgcgcag
     5521 tttctgaccg tgttacagtt atccgtcgtg ggaaatcaat tgaaaccgtt gaaattgcag
     5581 gggctaccaa tgctgatttg gcggaaatga tggtaggacg ttctgtttcc tttaaaacag
     5641 agaagcaagc ctctaaacca aaagaagtgg ttttgtctat caaagatttg gtggtcaatg
     5701 aaaaccgtgg tgttccagct gttaaaaatc tgtccttgga tgttcgtgct ggagagattg
     5761 ttggtattgc ggggattgat ggaaatggtc agtctgaact gattcaagcc attacaggtc
     5821 ttcgtaaggt tgaatctggt agcattgagc taaaagggga ttcaattgta ggcttgcacc
     5881 cacgtcagat tacagaacta agtgttgggc acgttccaga agaccgtcac cgtgatggct
     5941 tgattttgga aatgatgata tctgaaaata ttgcccttca aacctactat aaagaaccac
     6001 atagtaaaaa tggaattttg aattattcaa atattacttc ttatgctaaa aagctgatgg
     6061 aagagtttga tgttcgcgct gccagtgaat tagttcctgc agctgcactc tcaggaggaa
     6121 atcaacaaaa agcaattatt gctcgtgaaa ttgatcgaga tcctgatctc cttatcgtta
     6181 gccagccaac tcgtgggttg gatgtcggtg ccattgagta tatccacaaa cgcttgattg
     6241 aagagcgtga taatggcaag gctgtccttg ttgtcagctt tgaattggat gagattttaa
     6301 acgtctcaga ccgtattgcc gttatccacg atggtaagat tcaaggtatt gtatcaccag
     6361 aaacaaccaa taaacaagaa cttggtgtct tgatggctgg tggaaacttg ggaaaggaga
     6421 agagtgatgt ttaaaaaatt acaacaaatt tcggttccct tgatttctgt atttctagga
     6481 attttactcg gagccattgt catgtggatc ttcggttatg atgctatttg gggctacgaa
     6541 gaattgttct atacagcctt tggcagtctg cgtgggattg gagaaatctt ccgtgctatg
     6601 ggtcctctgg tcttgattgg tcttggtttt gcggttgcca gtcgagctgg tttctttaac
     6661 gtcggacttc ctggtcaggc tttggcaggt tggattctca gtggttggtt tgcgctgtcg
     6721 catccagata tgccccgtcc cttgatgatt ctagcaacca tcgtgattgc cttgattgct
     6781 ggtgggattg tcggagcgat tccaggtatt cttagggcct atctagggac gtcagaggtt
     6841 attgtaacca tcatgatgaa ctacattgtc ttgtatgtag ggaatgcctt tatccatgct
     6901 ttccctaaag acttcatgca aagtacagat tcgaccattc gtgttggggc taatgcaacc
     6961 tatcagacac cttggttggc tgagttgact ggtaactcac ggatgaatat tggtattttc
     7021 tttgccatca ttgccgttgc agttatttgg ttcatgctca agaaaacaac tcttggtttt
     7081 gaaatccgtg cagttggtct taatccacat gcttcagaat atgctggtat ttctgccaag
     7141 cggactatta tcctatctat gattatttca ggtgccttgg caggtcttgg tggagctgtt
     7201 gaaggtttgg gaaccttcca gaacgtctat gttcaaggtt cgtcattagc tatcggattt
     7261 aacggaatgg cggttagttt gcttgcggcc aactcaccaa ttggtatact ctttgcagcc
     7321 ttcctatttg gcgttctcca agttggggct cctggtatga atgcggcgca ggtaccatct
     7381 gagcttgtca gcattgtaac agcgtctatt atcttctttg tcagtgttca ttaccttatc
     7441 gaacgctttg ttaaaccgaa aaaacaagtt aaaggaggta agtaaagatg tctattataa
     7501 ccttgctccc cctcttggtg tcttctatgc tgatttactc agcacctctc atctttacaa
     7561 gtatcggtgg tgttttctct gaacgtggtg gtgtggtaaa cgttggcctt gaaggaatta
     7621 tggttatggg tgccttttct ggagttgtct ttaaccttga atttgcagaa caatttggag
     7681 cagcaactcc atggctatcc ttgcttgtag caggattggt tggtagtgtt ttctctatca
     7741 tccacgcagc agcgacggtt catttccgtg cagaccatgt tgtcagcggt acggtattga
     7801 acttgatggc gcctgccttg gctgttttct tagttaaagt tctttataac aaaggacaaa
     7861 ccgacaacct aagtcaaact tttggacgct ttgatttccc agtcttggca aatatcccag
     7921 tgattggtga tatcttcttc aagtcaacta gtctacttgg ttatctggcg attgccttct
     7981 cattccttgc ttggtttatt ctctttaaga ctcaatttgg tcttcgtctc cgctctgtcg
     8041 gtgaacatcc tcaagcagcg gataccttgg gaatcaacgt ctacaagatg agatatttag
     8101 gggttattat ttcaggtttt ctaggtggaa ttggcggagc gatttatgcg caatcaatct
     8161 cagttaactt ctcagtgaca actattgttg gacctggatt tatcgccctt gctgcgatga
     8221 tctttgggaa atggaatcca atcggtgcta tgctttctag tctcttcttt ggactttcac
     8281 aaagtttggc tgttatcggt tctcaattgc cgtttctaca aggagtgcca gcggtttatc
     8341 ttcaaattgc accttatgtt ttgacaattc ttgtcttagc agccttcttt ggaaaagcag
     8401 tcgcaccaaa agcagatggt atcaactaca tcaaatcaaa ataagcatac aaaaaaacgt
     8461 cagtccaatg actgaactgc cccccaaaag ttagacagaa aaaatctaac ttttggggtg
     8521 tttttattat gaaattaagt tatgatgata aagttcagat ctatgaacta agaaagcaag
     8581 gatatagctt agagaagctt tcaaataaat ttgggataaa taattttaat attaggtaca
     8641 tgattaaatt gattgatcgt tacggaatag agttcgtcaa aaaaggaaaa aatcgttact
     8701 attctcctga tttaaaacaa gaaatgatta ataaagtctg acatgaaggc tggactaaag
     8761 atagagtttc tcttgaatac ggtctcccaa gtcgtacgat acttcttaac tggctagcac
     8821 aatacaggaa aaacgggtat actattgttg agaaaccaag agggagagta cctaaaatgg
     8881 gacgtaatcc aaaaacgaga cctgaagaga ggacagaatt agaacgtctt caagcagaaa
     8941 atgagtacct gagagcggag aatgccatcc taaaaaagtt aagagaactc cgattgaagg
     9001 aggaaaaaga gaaagaagaa agacagaaat tgttcaagaa ttaatgactg agttttcgtt
     9061 agatcttctt ctaaaagcca ttaaactagc tcgttcgacc tactactatc acttgaaaca
     9121 gctagacaaa acagataagg accaagagct taaagctgaa attcaatcca tctttatcga
     9181 acacaaggga aattatgctt atcgtcggat ttatttagaa ctaagaaatc gtggttatct
     9241 ggtaaatcat aaaagagttc aacacttgat gaagtactca atttacaagc taaaacgcga
     9301 cagaaacgaa aatattcttc tcataaagga gacgttggca agaaagcaga gaatctcatt
     9361 caaggccaat ttgaaggctc taaaacaatg gaacagtgct acacagatgt gactgaattt
     9421 gccattccag taagtactca aaagctttac ttatcaccag ttttagatgg ctttaatagc
     9481 gaaattatcg cctataatct ttcaacttca cccaacttag aacaagtaca aacaatgttg
     9541 gaacaggcat tcacagagaa gcactacgag aatacgattc tccatagtga ccaaggctgg
     9601 caatatcaac acgattctta tcatcggttc ctagagagta agggaattca agcatctatg
     9661 tcacgcaagg gaaacagcca agacaacggt atgatggaat ctttctttgg cattttaaaa
     9721 tccgaaatgt tttatggcta tgagaaaaca tttaaatcac ttaaccaatt ggaacaagcc
     9781 attatagact atattgatta ctacaacaac aaacgaatta aggtaaaact aaaaggactt
     9841 agccctgtgc agtacagaac taaatccttt ggataaatta attgtctaac tgtttggggg
     9901 cagtacagaa ctggcgtttt attttttagg atcttgatgg gttaggttca atccccaagg
     9961 gaccaaattt tcagttttat ttttgatacg agctatattg tccttatgac gaatgataat
    10021 caaactagca agtgctaaga taatagcgat gaagagagag tcatagttac tcaggataaa
    10081 accaaaaagt ggaaagagca gaaccccgat aacagccgcg atcgatgctg tgacactaga
    10141 cagtgaaatc atactgccaa gatagagagc tccaaagaag ataatcgcaa ggtagagaca
    10201 gaagataggc gcaaatccga aaatcactcc agcactggtt gcgacagcct taccaccttt
    10261 aaatcctgca aagataggga aggtatggcc gataacagcc aaaagtccaa agatgagagg
    10321 agaaacgcct tgtagatgaa aaataatcgg aagcagcgtt gctagggttc ctttgaaaaa
    10381 gtcaatcaca aaggttgcca taccagcttt cttacctaaa atgcggaagg tgttggtcgt
    10441 tccagtgtta ccagaaccat gctcgcgtag attgatttga aagaatactt gtccaatcca
    10501 gagaccagat ggaatcgaac ccagcagata ggctaggatt aataaaacta ttgtaatcat
    10561 actcctatta tatcatgaat tgggaaagaa aagcagagaa tctctgctga aattgtcaca
    10621 tcggagaaag aaaaattttg caaaatcctt ggaaaacctg tagaatagta aagatgaacg
    10681 aataggaggt tccttgtgtc aaaaaaggaa atcaatatta acaattataa tgatgatgct
    10741 attcaggtgc tagaagggtt ggatgcggtc cgaaaacgtc cagggatgta tattggatcg
    10801 accgatggcg ctggtcttca tcacctagtt tgggaaatcg ttgataatgc agtcgatgaa
    10861 gccttgtctg ggtttggtga tcgtattgat gtaactatca ataaagacgg tagtctaacg
    10921 gttcaagacc atggacgtgg gatgccgaca ggtatgcacg ctatgggaat tccaactgtt
    10981 gaggttatct ttaccattct tcatgccgga gggaaattcg gtcaaggtgg ctataagaca
    11041 tcaggtggac ttcacggagt gggttcttcc gttgttaacg ccctttctag ctggttagaa
    11101 gttgaaatta cccgtgatgg cgcagtttac aagcaacgtt ttgaaaatgg tggaaaacct
    11161 gtcacgactt tgaagaaaat cggtacagca ctcaagtcta aaacaggcac caaagttact
    11221 tttatgcctg acgcgactat cttttctacg acagatttca agtacaatac catttcagag
    11281 cgccttaatg aatcagcctt tctcttgaaa aatgtgacct tgtctttaac ggacaagcga
    11341 acagatgaag cgattgagtt ccactatgag aatggagtac aagattttgt ttcttatctc
    11401 aacgaagata aggaaatctt gacgccagtt ctttactttg aaggggaaga caatggtttt
    11461 caagtggaag tagccctcca gtacaatgac ggattctcag ataacattct atcctttgtc
    11521 aataacgttc gcaccaagga cggtggaacg cacgagacag gactcaagtc tgccattacc
    11581 aaggtcatga atgactatgc acgtaaaaca ggtcttctca aggaaaaaga taaaaacctt
    11641 gaaggttcag actatcgtga gggactagcg gccgttcttt ctatcttagt tcctgaagaa
    11701 cacttgcagt ttgaaggaca gaccaaggat aaactaggaa gccccctagc tcgcccagtt
    11761 gtggatggaa tagtggctga taagttgacc tttttcctta tggaaaatgg ggaattagct
    11821 tctaacctca tccgcaaggc tatcaaggcc cgtgatgctc gtgaagcagc acgtaaggcg
    11881 cgtgatgaga gccgaaatgg gaagaaaaac aagaaagata agggcttgtt gtctgggaaa
    11941 ttgaccccag cccaatctaa gaatcctgct aagaatgaac tctatctagt tgagggggac
    12001 tctgccggtg gttctgccaa acaaggtcgt gaccgcaagt tccaggctat tctacctctt
    12061 cgtggtaagg ttgtcaatac agccaaggcc aagatggcgg atatcctcaa aaatgaagag
    12121 atcaatacca tgatttatac cattggtgcg ggtgttggag cagacttctc tattgaagat
    12181 gccaactatg ataagatcat tatcatgacc gatgcggata ccgacggtgc ccatatccag
    12241 accttgctct tgacattttt ctaccgttac atgcgtccgc tagtcgaggc aggtcatgtc
    12301 tatattgccc tcccacctct ttacaagatg tccaaaggta aaggcaagaa agaagaagtg
    12361 gcctacgctt ggacggacgg agaactagaa gaactccgta aacagttcgg taaaggcgct
    12421 accctccaac gatacaaagg acttggtgag atgaatgcgg accagctctg ggaaacaacc
    12481 atgaacccag aaacacgtac cctcatccgt gtcacaattg aagatttagc gcgcgccgaa
    12541 cgccgcgtca atgttctcat gggagataag gtagaaccac gccgtaaatg gattgaagat
    12601 aatgtcaagt ttacgctaga agaagcgaca gtgttttaat gaaagaaaat aatatgttag
    12661 taaactggaa attagagtcg gatgtgaatg actatgtgaa aaagcagttt gaaaatcttg
    12721 gtttgaaaaa attgcaggat tattaatgaa gaatctgcta tgagtgctta tctcaaagag
    12781 gcgtttaagg gagcggctaa gacacaaact aagactaatt ttggaaaacc atattttcat
    12841 attgaaaaat acaaacagga tagtttggtt acttatcgta actgaacacg ggacgaattc
    12901 ccgtaaaata tcttttactg cataaaaacc tactctacat tctttgaaag gagttgaaca
    12961 cgccctagat actgtgtgaa aaagataaat tctcttgtga gtttgcttac ttcaagaatt
    13021 ttctattttc acttggtatt ttatgggctt tgtatcttat gtctaacatt caaaacatgt
    13081 ccctggagga catcatggga gagcgctttg gtcgctactc caagtacatt attcaagacc
    13141 gggctttgcc agatattcgt gatgggttga agccggttca gcgccgtatt ctttattcta
    13201 tgaataagga tagcaatact tttgacaaga gctaccgtaa gtcggccaag tcagtcggga
    13261 acatcatggg gaatttccac ccacacgggg attcttctat ctatgatgcc atggttcgta
    13321 tgtcacagaa ctggaaaaat cgtgagattc tagttgaaat gcacggtaat aacggttcta
    13381 tggacggaga tcctcctgcg gctatgcgtt atactgaggc acgtttgtct gaaattgcag
    13441 gctaccttct tcaggatatc gagaaaaaga cagttccttt tgcatggaac tttgacgata
    13501 cggagaaaga accaacggtc ttgccagcag cctttccaaa cctcttggtc aatggttcga
    13561 ctgggatttc ggctggttat gccacagaca ttcctcccca taatttagct gaggtcatag
    13621 atgttgcagt ttacatgatt gaccacccaa ctgcaaagat tgataaactc atggaattct
    13681 tacctggacc agacttccct acaggggcta ttattcaggg tcgtgatgaa atcaagaaag
    13741 cttatgagac tgggaaaggg cgcgtggttg ttcgttccaa gactgaaatt gaaaagctaa
    13801 aaggtggtaa ggaacaaatc gttattactg agattcctta tgaaatcaat aaggccaatc
    13861 tagtcaagaa aatcgatgat gttcgtgtta ataacaaggt agctgggatt gctgaggttc
    13921 gtgatgagtc tgaccgtgat ggtcttcgta tcgctattga acttaagaaa gacgctaata
    13981 ctgagcttgt tctcaactac ttatttaagt acaccgacct acaaatcaac tacaacttta
    14041 atatggtggc gattgacaat ttcacacctc gtcaggttgg gattgttcca atcctgtcta
    14101 gctacatcgc tcaccgtcga gaagtgattt tggcgcgttc acgctttgac aaagaaaagg
    14161 ctgagaaacg tctccatatc gtcgaaggtt tgattcgtgt gatttcgatt ttggatgaag
    14221 tcattgctct tatccgtgct tctgagaata aggcggacgc caaggaaaac ctcaaagtta
    14281 gctatgattt tacggaagaa caagctgagg ctatcgtaac tttgcaactg taccgtttga
    14341 ccaataccga tgtggttgtc ttgcaggaag aagaagcaga gcttcgtgag aagattgcta
    14401 tgctggcggc tattatcggt gatgaaagga ctatgtacaa tctcatgaag aaagaacttc
    14461 gtgaggtcaa gaagaaattt gcaactcctc gtttgagttc tttagaagac actgcgaaag
    14521 caattgagat tgatacagct agtcttatcg ctgaggaaga tacctacgtc agcgtgacca
    14581 aggcaggtta catcaagcgt accagtccac gttcctttgc ggcttccacc ttggaagaaa
    14641 ttggcaagcg tgatgatgac cgtttgattt ttgttcaatc tgccaagaca acccagcacc
    14701 tcttgatgtt cacaagtctt ggaaatgtca tctacagacc aatccatgag ttggcagata
    14761 ttcgttggaa ggacatcgga gagcatctga gccaaaccat cacaaacttt gaaacgaatg
    14821 aagaaatcct ttatgtggaa gtactggatc agtttgacga tgcgacaacc tactttgcag
    14881 tgactcgcct tggtcaaatc aaacgggtag agcgaaaaga attcactcca tggcggacct
    14941 atagatctaa gtctgtcaag tatgctaagc tcaaagacga tacagatcag attgtagcag
    15001 tggctccgat taaactagat gatgttgtct tggttagtca aaatggttat gccctgcgtt
    15061 tcaatatcga agaggttccg gttgtcggtg ctaaggcagc aggtgtcaag gctatgaatt
    15121 tgaaagaaga tgatgtcctc caatctggct ttatctgtaa tacttcgtcc ttctacctct
    15181 tgacccagcg tggaagcttg aaacgtgttt ctattgagga aattctagca accagccgtg
    15241 ccaaacgagg attacaagtc ttgcgtgagt tgaaaaacaa accgcatcgt gtcttcttgg
    15301 caggagcagt tgcagagcaa ggatttgttg gcgatttctt cagtacggaa gtggatgtga
    15361 acgaccaaac tctgcttgtc caatccaata aaggaacaat ctatgaaagc cgattgcaag
    15421 acttgaactt gtcagaacgc actagcaatg gaagcttcat ttctgacacg atttcagatg
    15481 aagaagtttt tgacgcttat cttcaggaag tagttactga atataaataa gtgaaatgaa
    15541 gaataagttc taagagctgg tttttattgg a
//
LOCUS       DAVGJL010000039        15542 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.039, whole genome shotgun sequence.
ACCESSION   DAVGJL010000039 DAVGJL010000000
VERSION     DAVGJL010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15542
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.039"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..560
                     /locus_tag="VVE42_000985"
     CDS             <1..560
                     /locus_tag="VVE42_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lanthionine synthetase"
                     /protein_id="HEU3073194.1"
                     /translation="NVNSLKECNFAHGLAGIAYVILIYNSEFEFKVAYENSIKVFNNL
                     LENLLENEYSRLNATPTNLELSWCEGTSGILLYFELLYDKRYSNIISRFQQLAFKFNL
                     IQSSCYCHGLSSLLQTLNYQDKLNLRENVIEILLSRSKRDKNGILVFECEESSDTLFD
                     FGIGNLGIYWSILGESFPFEFKKEL"
     gene            560..1786
                     /locus_tag="VVE42_000986"
     CDS             560..1786
                     /locus_tag="VVE42_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU3073195.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLA
                     ILLITISETLPQVLQIFLGVLADFQHHRVLKYTVISFTKFLLYSIVSLSLSGQSFSLL
                     LVTFICLLNLLSDTLSYFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTISYQEGLSVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPISTLFLRSQPFLNLQTGQSLALLATLEL
                     SALILGSLMSGYLQHTISIKTALYASLVIQLLLLVGFATVRFDWILIFSTLDAFFAGI
                     LSPRLQELVFKQIPEESMGAVQSSISAITVVLPSLFTISLVTIATSFGTLAVSFVLLL
                     FLLIAFVMLLNIRESI"
     gene            complement(2139..2216)
                     /locus_tag="VVE42_000987"
                     /pseudo
     CDS             complement(2139..2216)
                     /locus_tag="VVE42_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            2287..2943
                     /locus_tag="VVE42_000988"
     CDS             2287..2943
                     /locus_tag="VVE42_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEU3073196.1"
                     /translation="MPTALGYVSIGLACGIIGAPYVTPVEMGLMSLFVYAGSAQFAML
                     ALIVVQAPVAAIAMTVFLINLRLFLLSLHASTYFRHTSLWYNIGMSSILTDETYGVLM
                     GELAHTDKVNPMWMHGNNLNSYVAWFVGTVVGTALGGLLPNPEIFGLDFALVGMFIGI
                     FASQFQIMQRRIPVRNLLIILAVVAVSFFLLLTVVSQSLAVLFATLLGCSMGVVLDGQ
                     "
     gene            2933..3256
                     /locus_tag="VVE42_000989"
     CDS             2933..3256
                     /locus_tag="VVE42_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEU3073197.1"
                     /translation="MVSKYLLLAVIFSGLVTWILRMIPFILVKYKGLPAIVERFLKFL
                     PVSIIFALILSSVVTGKVGSLPQIKWLDFLAVFPTAWVAFRYRNLVGTVLFGVVLIAI
                     LRLVF"
     gene            3360..4190
                     /locus_tag="VVE42_000990"
     CDS             3360..4190
                     /locus_tag="VVE42_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU3073198.1"
                     /translation="MKKIVKYSSLAALGLVAAGVLAACSGGAKKEGEAASKKEIIVAT
                     NGSPRPFIYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYNMAV
                     NNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
                     EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAQGQDELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            4344..5198
                     /locus_tag="VVE42_000991"
     CDS             4344..5198
                     /locus_tag="VVE42_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU3073199.1"
                     /translation="MKIKKWLGLAALATVAGLALAACGNSEKKADNATTIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVAIADTYISPIRLYSGLNGSANKYTKVEDIPANGEIAVPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKALFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEESS
                     DGLDQPVW"
     gene            5298..6671
                     /locus_tag="VVE42_000992"
     CDS             5298..6671
                     /locus_tag="VVE42_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU3073200.1"
                     /translation="MVFPSEQEQIEKFEKDHVAQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVW
                     TEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEE
                     VSRIYGLELPLLQEERMAFLKRFFFEPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYYTHIELVE
                     ELIRSYE"
     gene            6664..7725
                     /locus_tag="VVE42_000993"
     CDS             6664..7725
                     /locus_tag="VVE42_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEU3073201.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSGLSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEEGSVLEIFSNPKQPLTQDFISTATGIDEAMVKIEKQEIVEHLSENSL
                     LVQLKYAGASTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGVQ"
     gene            7727..8419
                     /locus_tag="VVE42_000994"
     CDS             7727..8419
                     /locus_tag="VVE42_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU3073202.1"
                     /translation="MESLIQTYLPNVYKMGWAGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAVPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIIIILIIFAIQF
                     LGDFLTKKLSHK"
     gene            complement(8449..9018)
                     /locus_tag="VVE42_000995"
     CDS             complement(8449..9018)
                     /locus_tag="VVE42_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEU3073203.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPRFGAITTIGLVIAFFFLGTKHGAGSFLPGIICGLLADEVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVAPTPGTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
     gene            9153..9767
                     /locus_tag="VVE42_000996"
     CDS             9153..9767
                     /locus_tag="VVE42_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEU3073204.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLAGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFVSSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVALIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKVLLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            9769..11415
                     /locus_tag="VVE42_000997"
     CDS             9769..11415
                     /locus_tag="VVE42_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU3073205.1"
                     /translation="MKQFWLHTLLRTYSSVMIIIIASFAILLSYADWDSREKEAQRVA
                     QRVTARTVSEIEYYHRESTQIAQALVENQARIEGIYKYFSLSMPDYFYWQLERKASPY
                     ISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDFKPAGNSFA
                     IPVSDPVSDQDLGVIYISLNPAVLYHAIDNTRGHTPMAVTVTSPFDTEIFHIGETVDR
                     ESENWLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLTLRQTFANY
                     QKQVVDLVESIQVIAQGEEGRRIDISEKDQELLLIAETTNDMLDRLEKNIHDIYQLEL
                     SQKDANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLRNNISDERE
                     TLLKQELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVENYFAHGVD
                     HRRTDNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQKYFEHQASYSDQRQ
                     SIGIVNVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            11427..12713
                     /locus_tag="VVE42_000998"
     CDS             11427..12713
                     /locus_tag="VVE42_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU3073206.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATASHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAGFVSYLGDKENWWIGLSKEK
                     QGSFTIPYYVLGQDWQIFISGQPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRVPITKQLFIQFVMDVF
                     HLFEHLKADDLTDIVKTIHAIQSFDELVPYIKETLTSFFGQYRMNENVVSVLEVIGRD
                     YQKELSLKDMSKDLFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICYAVGYSNLGYFYKVFRKLCGKSPKAYRKQVETIL"
     gene            12765..13298
                     /locus_tag="VVE42_000999"
     CDS             12765..13298
                     /locus_tag="VVE42_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU3073207.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRAAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDESMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKALAEEEETEELA"
     gene            13376..13846
                     /gene="nrdI"
                     /locus_tag="VVE42_001000"
     CDS             13376..13846
                     /gene="nrdI"
                     /locus_tag="VVE42_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEU3073208.1"
                     /translation="MKTISLVYISLSGNTESFVTRLKDYLLSQYKGIEVQKIHIKDLV
                     KEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN"
     gene            complement(14139..15401)
                     /locus_tag="VVE42_001001"
     CDS             complement(14139..15401)
                     /locus_tag="VVE42_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEU3073209.1"
                     /translation="MSQAISLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFGAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSAM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     AEKRKTVNFNTEDALFDFNTGYIGTAILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIVIIKFFAGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIGKAG"
BASE COUNT         4527 a         2889 c         3447 g         4679 t
ORIGIN      
        1 gaaatgttaa tagtttaaaa gaatgtaatt ttgcacatgg tctagctgga attgcatacg
       61 taattttaat ttataattcg gaatttgaat tcaaggtagc ttacgaaaat agtattaagg
      121 tattcaataa tctgttagaa aatttgttag aaaatgaata tagtagacta aatgcaactc
      181 ctacaaattt agaactatcg tggtgcgaag gaacttcagg aatcttactt tattttgagt
      241 tattatatga caaaaggtat tcaaacataa tttcaaggtt tcaacaactt gctttcaaat
      301 tcaatcttat ccagtcaagt tgttattgtc atggcttatc cagtttgttg caaactctaa
      361 actatcagga taaattaaat ttacgtgaga atgtgattga gattttatta agtagatcca
      421 aaagagataa aaatggtatt ttggtatttg aatgtgaaga aagttcagat acattatttg
      481 attttggtat cggaaatcta gggatttatt ggtctatcct tggggaaagc ttcccatttg
      541 aatttaagaa ggagttgtga tgaaagtatt tcttcaaaat agagatttta gacaattaac
      601 catcaaccag tggatttcaa cgcttgggga tacgattttt tatctggcct tcctgaatta
      661 tgtggcagat gcgtcttttg cccctttggc gattttactt atcacgattt cagaaaccct
      721 tccccaagtt ctacaaatct ttctgggagt tttggcggat tttcaacatc atcgtgtctt
      781 gaaatacaca gtcattagtt ttacaaaatt tttgctttac tctatagtgt ccctatcgct
      841 ttcagggcag tctttttcct tgttgctagt aacctttatt tgtctgctta acctcttgtc
      901 tgacacattg agttattttt caggcgccat gctcactccg attttcatta gaattattgg
      961 gcaagaccat ctggcagaag ctattggatt taaacagtca actgttagtt tagtgaaaac
     1021 aatcagtaat atcctaggag gagtcttact aggcattcta tccatccagt ttatttcctt
     1081 actgaacgct cttacctttt taatcgcatt tttaggtatc ctcttcataa aaactgacct
     1141 cttgaaagta gaaaaaacga ttagctatca agaaggactc tctgtaaaat ccttttgcca
     1201 gcatttgctc caatcatcga aattgatatg gaatatgaat aaggtgctct tggttttgtt
     1261 tattatctct actagtcaag cagtgataaa tgttacggtt cctatttcca ctctattttt
     1321 aaggagccag ccctttttga atttacaaac tggtcaatct cttgccttgt tagccacact
     1381 agaattatca gcccttattt tgggaagtct tatgagtggc tatctgcaac ataccatttc
     1441 cataaaaaca gctctctatg cctcacttgt catccagttg cttcttctag taggatttgc
     1501 cacagttcgt tttgactgga ttctcatctt tagtaccttg gatgcctttt tcgcgggtat
     1561 tctctctcct agattgcaag aactcgtctt taaacaaata cctgaggagt caatgggagc
     1621 agttcaatct tctatcagtg ccattacggt tgttttacct agcttattta caatatcttt
     1681 ggtaaccatt gctactagct ttggaactct ggcagttagc tttgttttat tgctatttct
     1741 tctaattgcc tttgtcatgc ttctgaatat tcgtgaaagt atttagtgga gaatttctat
     1801 aatatatgtt tgagactgga ttgccagtct ttttatgatg gtcaaaatca acagactttt
     1861 ttctcttttc tgaatgtgtt atagtagact gaatctaaaa tagtacgaaa caattgctaa
     1921 aacatttata gaaattaatt ttactttccc aatcgatttg ttctcatctt atttcaatct
     1981 gctatataaa atagtccatg ctgtggctgg attcttttca gcctacttat actcttcgaa
     2041 actctcttca aaccacgtca gcttcacctt gccgtatata tgttactgac ttcgtcagtt
     2101 ctatccacaa cctcaaaacg gtgttttgag ctgacttcgt cagttctatc cacaacctca
     2161 aaacagtgtt ttgagcaacc tgcggctagt ttcctagttt gctctttgat tttcattgag
     2221 tattattaag acaatatgag gagaaagatg aaagaaaaag gattttagga gggggtgcag
     2281 gcagtcatgc caacggctct tggttatgtc agtatcggcc tggcctgtgg aattatcggt
     2341 gcgccctatg tgacacctgt tgagatgggc ttgatgagtc tctttgttta tgctgggagt
     2401 gcccagtttg ccatgttggc actgattgtg gttcaagctc ctgtggcagc tattgctatg
     2461 acggtttttc taatcaactt gcgtctcttt ttgttgagtt tacacgcatc gacttatttc
     2521 cgtcatacca gtctttggta caatatcggt atgtctagta tcttgacaga tgagacctat
     2581 ggcgttttga tgggtgaatt ggcccataca gacaaggtaa atcctatgtg gatgcacgga
     2641 aacaatctta acagctatgt ggcttggttt gtggggacag tagtcggaac ggctctgggt
     2701 ggcctgctac caaatccaga aatctttggc ttggattttg ccctggttgg gatgtttatt
     2761 ggtatttttg cttcgcaatt tcagattatg caaagacgga ttcctgtccg caatctgctc
     2821 attatcctag cagttgttgc ggtgtccttc tttttgctct tgacagtggt gtctcagtca
     2881 ctagctgttc tgtttgcgac gctacttggt tgtagcatgg gggtggtttt agatggtcag
     2941 taagtatctt ttattagcag ttattttctc tggcttggtg acttggattc tccgtatgat
     3001 tcccttcatc ttagtcaagt ataagggctt gcctgcaatc gttgagcgtt ttttgaagtt
     3061 cttgcccgtt tccattatct ttgccttgat tctttcaagc gtagtgacag gtaaggttgg
     3121 gagccttcct caaattaaat ggctagactt cttagccgtc tttccaacag cttgggtagc
     3181 ctttcgctac cgcaatctag tcggaacagt tctctttgga gtggtcttga ttgccatctt
     3241 gcgtttggtc ttttaaatta cccataaaaa aaacttatca cagagataga tatcatataa
     3301 tggcgtaaat gctctttttc tgttaagatt ataaggtatt ctattttgga ggaaatgaca
     3361 tgaaaaaaat cgttaaatac tcatctcttg ctgccctagg acttgttgct gcaggtgtgc
     3421 ttgcggcttg ctcagggggt gctaagaaag aaggagaagc agctagcaag aaagaaatca
     3481 tcgttgcaac caatggatca ccaaggccat ttatctatga agaaaatggc gaattgactg
     3541 gttacgagat tgaagtcgtt cgcgctatct ttaaagattc tgacaaatat gatgtcaagt
     3601 ttgaaaagac agaatggtca ggtgtctttg ctggtcttga cgctgaccgt tacaatatgg
     3661 ctgtcaacaa tcttagctac actaaagaac gtgcggagaa atacctctat gccgcaccaa
     3721 ttgcccaaaa tcctaatgtc cttgtcgtga agaaagatga ctctagtatc aagtctctcg
     3781 atgatatcgg tggaaaatcg acggaagtcg ttcaagccac tacatcagct aagcagttag
     3841 aagcatacaa tgctgaacac acggacaacc caactatcct taactatact aaggcagact
     3901 tccaacaaat catggtacgt ttgagcgatg gacaatttga ctataagatt tttgataaaa
     3961 tcggtgttga aacagtgatc aagaaccaag gtttggacaa cttgaaagtt atcgaacttc
     4021 caagcgacca gcaaccgtac gtttacccac ttcttgctca aggtcaagat gagttgaaat
     4081 catttgtaga caaacgcatc aaagaacttt ataaagatgg aactcttgaa aaattgtcta
     4141 aacaattctt cggagacact tatctaccgg cagaagctga tattaaataa tttcttgaaa
     4201 tcatccattc tgagtaaggt gggtggtttc tcagttttta gggatatagt tttaaactat
     4261 atatctgact atctaataac aatttgtgaa atcaaacaaa ttgtgagata ctagtacggt
     4321 attattttta aggagaaaga atcatgaaaa tcaaaaaatg gcttggccta gcagcccttg
     4381 ctacagtcgc aggtttggct cttgcagctt gcggaaactc agaaaagaaa gcagacaatg
     4441 caacaactat caaaatcgca actgttaacc gtagcggttc tgaagaaaaa cgttgggaca
     4501 aaatccaaga attggttaaa aaagacggaa ttaccttgga atttacagag ttcacagact
     4561 attcacaacc aaacaaagca actgctgatg gggaagtaga tttgaacgct ttccaacact
     4621 ataacttctt gaacaactgg aacaaagaaa acggaaaaga ccttgtagcg attgcagata
     4681 cttacatctc tccaatccgc ctttactcag gtttgaatgg aagtgccaac aagtacacta
     4741 aagtagaaga catcccagca aacggagaaa tcgctgtacc gaatgacgct acaaacgaaa
     4801 gccgtgcgct ttacttgctt caatcagctg gcttgattaa attggatgtt tctggaactg
     4861 ctcttgcaac agttgccaac atcaaagaaa atccaaagaa cttgaaaatc actgaattgg
     4921 acgctagcca aacagctcgt tcattgtcat cagttgacgc tgccgttgta aacaatacct
     4981 tcgttacaga agcaaaattg gactacaaga aagcactttt caaagaacaa gctgatgaaa
     5041 actcaaaaca atggtacaac atcattgttg cgaaaaaaga ttgggaaaca tcacctaagg
     5101 ctgatgctat caagaaagta atcgcagctt accacacaga tgacgtgaaa aaagttatcg
     5161 aagaatcatc agacggtttg gatcaaccag tttggtaata agaaacaggg aggtgggaga
     5221 gagaattcca cctcttgctt ttgtatagag cacggagtgt aaagaagact attcgttcat
     5281 agaaaggtag agagaatatg gtttttccta gcgaacaaga acagattgaa aaatttgaaa
     5341 aggatcatgt agcccagcat tattttgagg ttttgcgtac cttgatttct aagaaatcag
     5401 tctttgccca gcaggttgga ctcaaggaag tcgcaaatta tctgggtgag attttcaagc
     5461 gtgttggagc tgaagtggag attgatgaga gctatacagc gccctttgtc atggcacatt
     5521 tcaagagttc gcgtccagat gccaagacct tgattttcta taaccactat gacactgtgc
     5581 cagcggatgg ggatcaggtc tggacagagg atccttttac gctttcggtc cgcaatggct
     5641 tcatgtatgg gcgtggggtt gatgacgaca agggtcatat cacagctcgc ttgagtgctt
     5701 tgagaaaata tatgcagcac catgatgatt tacctgtcaa tatcagcttt atcatggagg
     5761 gagcggagga atcggcttca acagacctag ataagtattt ggaaaagcat gcagacaaac
     5821 tccgtggggc ggatttgttg gtctgggaac aagggaccaa aaatgccttg gaacagctgg
     5881 aaatttctgg tggcaataag gggattgtga cctttgatgc caaggtaaaa agtgctgatg
     5941 tggatatcca ctcgagttat ggtggtgttg tggaatcagc tccttggtat ctcctccaag
     6001 ccttacagtc tcttcgtgct gcggatggcc gtatcttggt tgaaggcttg tacgaagaag
     6061 tacaagagcc caatgaacga gaaatggcct tgctagaaac ttatggtcaa cgaaacccag
     6121 aggaagttag tcggatttat ggattggagt tgcctctctt acaggaggag cggatggcct
     6181 ttctaaaacg tttctttttc gagccagcgc ttaatatcga aggaatccag tctggttatc
     6241 aaggtcaggg tgttaagact attttacctg cagaagccag tgccaagcta gaggttcgtc
     6301 tggttccggg cctagaaccg catgatgttc tggaaaaaat tcggaaacaa ctagacaaaa
     6361 atggctttga taaggtagaa ttatactata ccttgggaga gatgagctat cgaagcgata
     6421 tgagcgcacc agccattctc aatgtgatcg agttggccaa gaaattctat ccacagggcg
     6481 tttcagtctt gccgacgaca gcggggacag gacctatgca tacggtcttt gatgccctag
     6541 aggtaccaat ggttgcattc ggtctaggaa atgccaatag ccgagaccac ggtggagatg
     6601 aaaatgtgcg aatcgctgat tattacaccc atatcgaatt agtagaggag ctgattagaa
     6661 gctatgagta gagatattat caagttagat cagatcgatg tgacttttca ccaaaagaag
     6721 agaaccatca cagcggttaa ggatgtgacc attcacatcc aagaagggga tatctacgga
     6781 atcgttggat attctggagc agggaaatca acccttgtac gggtgattaa tctcttgcaa
     6841 aaaccatctg cagggaaaat taccattgac gacgatgtga tttttgacgg caaggtgacc
     6901 ttgacggcag agcagttgcg tcgtaaacgt caagatatcg ggatgatttt ccagcatttt
     6961 aacctgatga gccaaaagac agcagaggag aatgtagcct ttgcccttaa acactctgga
     7021 ctcagcaagg aagaaaagaa ggctaaagta gctaagttgt tggacttggt tggtttggca
     7081 gatcgtgctg aaaactaccc ttcacaacta tctggagggc aaaaacagcg tgtggcaatt
     7141 gcgcgtgcct tggccaatga tccaaaaatc ttgatttcag acgagtcaac ttctgccctt
     7201 gaccctaaga caaccaagca gattttggcc ttgttgcaag atttgaacca aaaattagga
     7261 ttgacagttg tcttgattac gcatgaaatg cagattgtca aagacattgc caaccgtgtg
     7321 gcggttatgc aggatgggca tttgattgaa gagggaagtg tccttgaaat cttctcaaac
     7381 cctaaacaac ctttgactca agactttatc tcaacagcca caggtattga cgaagccatg
     7441 gtcaaaatcg agaagcaaga aatcgtggaa cacttgtctg aaaacagtct cttggtgcaa
     7501 ctcaagtacg ctggagcttc aacagacgag ccacttttga atgaattgta caagcattac
     7561 caagtaatgg ctaatattct ctatgggaat atcgaaattc tcgatggtac tcctgttgga
     7621 gaattggtgg tggtcttgtc aggtgaaaaa gcagcgttgg caggtgccca agaagccatt
     7681 cgtcaagcag gtgtacaact aaaagtattg aagggagtac agtaagatgg aatcattgat
     7741 tcaaacctat ttaccaaatg tctataaaat gggttgggct ggtcaggcag gctggggaac
     7801 ggctatctac ttaactcttt atatgacagt tctttccttc attatcggag gcttcttggg
     7861 gctagtggca ggtctctttc tcgtcttgac agcgccaggt ggtgtcttgg agaataaagt
     7921 cgtattctgg attttagaca aaattacctc aatttttcgt gcggttccct ttatcatcct
     7981 cttggcaatc ttgtcaccac tttctcactt gattgttaaa acaagtatcg ggccaaatgc
     8041 agcccttgtc ccactttctt ttgcagtctt tgccttcttt gcccgtcagg tgcaggttgt
     8101 cttggctgaa ttggatggcg gtgtcattga ggcggctcaa gcgagcggag cgactttctg
     8161 ggacatcgtg ggtgtttacc tatcagaagg tcttccagat ttgatccgtg tgacgactgt
     8221 gaccttgatt tcccttgttg gggaaacagc tatggccggt gcggttggag ctggtggtat
     8281 cggtaacgta gccatcgctt atggatttaa ccgctacaat cacgatgtga ccatcttggc
     8341 aaccattatt atcattttga ttatctttgc aatccaattc ttgggagatt tcttgactaa
     8401 gaaattgagc cataaataaa aaagagccgc gtggctcttt ttaactgatc agattttctg
     8461 tgcaaatttt ttactcaagg cttgtccaat caaggcaccc actagggctc cgatgacaat
     8521 acttgcgata aatagaagga cagttccagg ggttggagcg accatgatac ggtcgatata
     8581 ttcttgggat tttcctcttg ccagaagagt agccatatag gctttgggcg caatccacat
     8641 aagcaagatt ggtcctgttg tactaaaggc gaaaataatg aaagaaagga agttctttgt
     8701 tttgtccttg tattttccta aatgagctac ttcatctgct aggaggccac agataattcc
     8761 aggaaggaag gaaccagcac cgtgtttagt tcccaagaaa aagaaggcaa tgacaaggcc
     8821 gatagtggta atggctccaa agcgcggaac ttttgcgact aggatcatat agacgctacc
     8881 gccgacaagg gcagtaaagg caggcgcata aaacatgttt ccagtttggt caaagagatt
     8941 gcccaaaagg acaccaatcc ccatgcagag gaagtaaaga actgcaaaaa gcagtgtagt
     9001 taagatagat ttcttcatga attgtctcct gataattttt tcacaattct cattttacca
     9061 tggtttggtg ggattgtaaa tggatactag aagtttttga aaacgcttga aatatgatat
     9121 aatggtttca tagttatttt taggggaata tcatgggtag atttttagac tttgtcttta
     9181 atcgtttctt tttagggatg attgcgacag ccttcttttg gctattaact ttagcaggag
     9241 ggattatcct tggtctagcg ccggctagtg ccaccttgat gagcttatat gcagaacatg
     9301 gttatagctt tcgggaatac agtttgaagg aggcttggtc tctttacaag caaaattttg
     9361 tctcaagcaa cctgattttc tatagctttt taggtgtggg tctagttttg acctatggtt
     9421 tgtatctctt ggtgcaattg cctcatcaga ccattgttca tttgattgcg acccttttga
     9481 atgtcctagt agttgccctg atctttttgg cttatacagt atctttaaaa ttacaagttt
     9541 attttgcctt gtcctatcga aatagtctca aattatcctt gattggcatc tttatgagtc
     9601 tagcagctgt ggccaaggtt ctccttggga ctgtgctact tgtagcaatt ggttactata
     9661 tgcctgccct gctatttttt gtaggaattg ggatgtggca tttctttatc agtgatatgt
     9721 tggaacctgt ctatgaaatc atccatgaaa aattggcgac aaaatagaat gaagcagttt
     9781 tggctacata cgcttctaag aacctatagt tcagtgatga tcattatcat tgcgagtttt
     9841 gcaatcttac tctcttacgc tgactgggat tcacgtgaaa aggaagccca gagagtagcc
     9901 cagcgtgtaa ctgctagaac agtgagtgaa attgaatatt accatagaga gtcaacccag
     9961 atagctcagg ctttagttga aaaccaagct cgtattgagg gaatctataa atactttagc
    10021 cttagcatgc cagactattt ttactggcaa ttagagcgga aagcttcgcc ttatatatca
    10081 gtctctctgt atgaaaatgt tgatgacctc tatgttcgaa atgattttgt aactggggtg
    10141 gccattgctt ttcaagatta caaggaagtc tatgtttcta ctaaagacaa acgtagtgga
    10201 gaaaaaatca gggctgagga tttcaaacca gcaggaaata gttttgccat tccggtgtca
    10261 gatccagtgt cagatcaaga tttaggagtg atttacatct ccttgaatcc tgctgtttta
    10321 taccatgcca ttgataatac tagaggtcat actccgatgg cagtaacagt gacctcacct
    10381 tttgatacgg agatttttca tattggtgag acagttgata gggagagtga aaattggcta
    10441 gttggcttaa cttctcatgg atatcaggtt caggtggcag ttcctaaaaa ctttgtttta
    10501 caaggaacag tgactagctc tgctttgatt gtgggtttga gccttctctt tattgtcatt
    10561 ctttatctga ctttgaggca gacttttgct aattaccaaa agcaggtagt ggatttagta
    10621 gaatccattc aagtcattgc tcaaggcgaa gaggggcgtc ggattgacat ttccgagaaa
    10681 gatcaggaat tactcctaat cgcggagacg accaatgata tgttggatcg attggaaaag
    10741 aatatccatg atatttacca gttagagctt agtcaaaaag atgccaatat gcgagccttg
    10801 caggcgcaaa tcaatcctca ttttatgtat aatacgctgg agttcttgcg catgtatgca
    10861 gttatgcaga gtcaagatga gttggcagat atcatttatg aattcagtag tctcttgcgt
    10921 aacaatattt ccgacgaaag agagaccctc ctcaaacagg aattagaatt ttgccgtaaa
    10981 tacagctatc tctgcatggt tcgctatccc aagtccattg cctatggttt caagatagat
    11041 ccagagttag agaatatgaa gattcccaag tttaccttgc aaccgctggt agaaaactat
    11101 ttcgcgcatg gtgttgacca caggcggaca gataatgtga ttagcatcaa ggctcttaaa
    11161 caggatggtt ttgtggaaat tttggtggtc gataatggta gaggaatgtc ggctgaaaag
    11221 ctggcaaata tccgagaaaa attaagtcag aaatattttg aacaccaagc cagctacagt
    11281 gatcaaaggc agtctatcgg gattgtcaat gtacacgagc gttttgtgct ctattttgga
    11341 gaccgctatg ccattactat agagtctgca gagcaagccg gtgttcagta tcgtattaca
    11401 attcaagatg agtagaaagg gagaaaatgt ataaagtatt attagtagat gatgagtaca
    11461 tggtgacaga aggtctgaag cgtttgattc cctttgataa gtgggatatg gaggtcgtcg
    11521 caacagccag tcatgccgat gaagctctag aatatgttca ggaaaatcct gtcgatgtca
    11581 tcatttccga tgtcaatatg ccagacaaaa cagggcttga tatgattcgg gagatgaaag
    11641 agatcttacc agatgctgcc tatatcctgc tctcaggtta tcaggagttt gattatgtaa
    11701 aaagagcaat gaaccttagt gtggtggact atttggtcaa gcctgttgat aaggtagagc
    11761 tgggaaatct gctggagaag attgcaggtc agctcggcga gagagggaag aaaagtcaga
    11821 ctcttagtca agaattagac gaggctggat ttgttagtta tttaggggat aaggagaatt
    11881 ggtggatagg tctatccaag gaaaaacaag gttccttcac cattccctac tatgtcttgg
    11941 gtcaagactg gcagattttc atttctggcc aacccctaga tggcttagtc gttactccct
    12001 ttgaagctcc ctatcaagaa cactttgaac gctggaagct gaatgctgag aaaaccctct
    12061 tttacggttc tgtaaatttg cagcagtctg agagtctctt tgcctattac gaaccgattt
    12121 atagggttat cattcaggga aatctcaatc aaatcgtaga agagttaaat ctcttggaga
    12181 aggtagttct tgaaaatacg ccgcgtgttc cgattactaa acagcttttt attcagtttg
    12241 tcatggatgt tttccattta tttgaacatc tcaaagctga tgacctgaca gacatcgtca
    12301 aaaccattca tgctattcaa tccttcgatg aattggttcc ttatatcaag gaaactctga
    12361 ccagcttttt cggtcaatac cgtatgaatg aaaatgtggt cagtgtgctg gaagtcattg
    12421 gtcgtgatta ccaaaaagag ctttccctca aggatatgag taaggatctc tttatcaatc
    12481 ctgtctatct agggcagttg attaagcgtg aaaccgattc gacttttgca gagttactaa
    12541 acaaacaacg tattaaggct gcccaacaac ttttgctttc aactagtgac agcatcgaag
    12601 atatttgtta tgctgttggt tacagtaacc ttggatattt ctataaagtt ttccgaaaat
    12661 tgtgcggaaa atcgccaaaa gcctaccgaa aacaggtaga aactatacta taagatttgt
    12721 attcctttac aaaatgtgct ataatattat caggaggtcc tatcatgaaa aagaaaccaa
    12781 tttatctatg ggtcttgcta atcttgtctg cccttatttc agttccgtct ctgtttggaa
    12841 ttgtgagtcc cttgcctagt aaagaagccc ttcgtgctgc tcagaaacaa gttgcagggg
    12901 tcaatgctca gcaattagaa gaccagctta attataccta tagagtagca gaagcatctc
    12961 attctatttt taatgttgct ttgattgtgc tatctactat tttagtggtc gtagcgattg
    13021 tcttccttgt tcgtaaaaat ttgcaatatg caaactatac ttacgttggc tatgttttgt
    13081 tagctattat tggttcgatt tatggctatg ttggtttaca agacgctgtg cagttggttc
    13141 aagatgagag catgcgtttg acagtgagta ttggttcaaa agctgttagc attttctata
    13201 tcgtcatcaa tgttctcttc ctagcccttg tcttctataa gatgtggcga caacagaaag
    13261 ccttggcaga agaagaggaa acagaagagc ttgcctaagt attgacaaaa aagaactatc
    13321 aagatacaat cttggtagtt gttttttgat tatagataaa atcatggagg tacatatgaa
    13381 gacaatttct ttagtttata ttagtttgag tggcaacact gaaagttttg tgacgcgctt
    13441 gaaagactat ctcttgtccc agtacaaagg gattgaggtt caaaagattc atatcaagga
    13501 tttagtcaag gaaggcaaaa atttctatga aatggaccat ccctatgtcg cttttttgcc
    13561 gacctatctc gaaggtggga atggcgtgga taacggagat gttgagattt tgacgacacc
    13621 ggtgggagat tttattgcct atggtaacaa tgctagtaag tgttttggtg tggttgggtc
    13681 aggaaatcgt aactttaata accaatactg cctaacagcc aagcaataca gtcaacgctt
    13741 tggcttccct gtattggctg actttgaaat gcgaggcatg ctggaagata tcaaacatgt
    13801 cgcagctatt atcgcagatt tgtatgagtt ggaaaaggag aattaatact caatgaaaat
    13861 caaagagcaa actaggaagc tagtcgcagg ttgctcaaag cacagctttg aggttgcaga
    13921 tagagctgac gtggtttgaa gagattctcg aatagtataa agttcttata agaggtggtt
    13981 gaagttgttc aactgttttt tgagtataaa cagtctttaa gaacgtaaaa aagcataagt
    14041 tcaggtcttc aaaaccttgt tcttatgctt tttatcatag aaatatttgc tgaattttct
    14101 cttgaaatga aaccttatct tcatctcgcg cttgtccctt accctgcttt tccgattgcg
    14161 agtgcgtaga taaagaagat ggcgaagcca aagaggaaaa tcaatcccgc aatggcaagg
    14221 tgtttgagcc aagaaggaag atttttgttt tcacgtgtta ccaaggcgta attcaaaaga
    14281 gcaaagaaag gtgttgtcag gaaagatcca atcatggcaa agcggagcat ggttgaaacc
    14341 tgaccagcga agaacttgat aatgacgata ccgatgatag cagtgatggt catccagatg
    14401 ttcaaagatt tacgattgtc ctctttttga ctgattagca gtcggagaga ttcctgatta
    14461 acgcgagaat agccatcgat aacagttata actgttccaa agatacagag gaaggcaata
    14521 aaggtaatca agtaacggga ccattcgcca agaacagagg catacatgcc cacgaattga
    14581 gagatgtatt tggctgaagc agcttcaacc gcctgccctg taggatactg aatcagtgct
    14641 cccagtgcca caaagaagac ggctaggata gctgttccaa tataaccagt gttaaagtca
    14701 aacagagcgt cttctgtgtt aaagttgacg gtctttctct tttcagctga ccaaagtgaa
    14761 ttgatggctg aaatttcaat aggagccggc atccatccta ggagggagac gatgaagggc
    14821 agagctgcca tttgccaagg tgtcttctcg acaaaatcag aactgtattc tggatgcttg
    14881 accgccgcaa tgataactgc aagaacagtc gcaatggtta aggcagacat aatccatttg
    14941 accatgccgt ctaaaagttt gtagcctcca aagagtagca tagcccaaat gattgcaacg
    15001 agaatgaggg accactgagt aatgctaagt ccaatcattg ggaaggcact ggcgatgata
    15061 gctgagcaca gaatggcaac accagccgtg ttgaccatag ccgaaaagac attgagaata
    15121 aagaaaatcc agagatagag ttttcctttt tcggcataac cttcaaccaa agtctttcca
    15181 gtatcagctg tgtattcagc accaaaacgg aaaaatggat atttaaagac attggctaag
    15241 atgaccaaga gaagtagaga ccaaccgtaa gaaccgccag cttgagttga ggatacaatg
    15301 tgggaacctc caacagcggc agttgccatt aggattccag gtcccattgc ttttagcttg
    15361 cttgcccagg ttgattgatt taaggaaata gcttgcgaca tgataaatac tcctttttga
    15421 attttcagaa taatctgtat atgcaatcta tattctacaa aaaattttgg aaaaatgcaa
    15481 gaatattttg aaaaaagata gaaaatttac aaagaaaatc tgaaataact gttatgatat
    15541 ag
//
LOCUS       DAVGJL010000040        15536 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.040, whole genome shotgun sequence.
ACCESSION   DAVGJL010000040 DAVGJL010000000
VERSION     DAVGJL010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15536
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.040"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            110..2167
                     /gene="pbp2b"
                     /locus_tag="VVE42_001002"
     CDS             110..2167
                     /gene="pbp2b"
                     /locus_tag="VVE42_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HEU3073210.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNK
                     DFYEKKLASASQTKITSSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKETA
                     KKLLTYVSISSPNLTERQLADYYLADPEIYKKIVEALPSEKRLDSDGNRLSESELYNN
                     AVDSVQTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKEMP
                     GISISTSWDRKVLETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELENGGAKYSEGVYAVALNPKTGAVLSMSGIKHDLKTGELTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDQSIVFQGSAPINSWYTQAYGSFPITAVQALEYSSN
                     TYMVQTALGLMGQTYQPNMFVGTSNLESAMEKLRSTFGEYGLGTATGIDLPDESTGFV
                     PKEYSFANYITNAFGQFDNYTPMQLAQYVATIANNGVRVAPRIVEGIYGNNDKGGLGD
                     LIQQLQPTEMNKVNISDSDMSILHQGFYQVAHGTSGLTTGRAFSNGALVSISGKTGTA
                     ESYVADGQQATNTNAVAYAPSDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYQKYH
                     PMN"
     gene            2178..2774
                     /gene="recR"
                     /locus_tag="VVE42_001003"
     CDS             2178..2774
                     /gene="recR"
                     /locus_tag="VVE42_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEU3073211.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSADDVNEFA
                     KNLLSAKRELTYCSICGRLTDDDPCSICTDPTRDQTTILVLEDSRDVAAMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
                     KPAGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            2950..3993
                     /locus_tag="VVE42_001004"
     CDS             2950..3993
                     /locus_tag="VVE42_001004"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEU3073212.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVNYDRFTVKTFFISQ
                     SGDFIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLAYPVFTKPSNMGSSVGISKSENQEELRQALKLAFRYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKITMDIPAKISDDVVAVMRQNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWDNMGISYPELIERLVDL
                     AKESFDKREAHLI"
     gene            4077..5450
                     /locus_tag="VVE42_001005"
     CDS             4077..5450
                     /locus_tag="VVE42_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEU3073213.1"
                     /translation="MKLTIHEIAQVVGAKNDISIFEDTQLEKAEFDSRLIGTGDLFVP
                     LKGARDGHDFIERAFENGAAATLSEKEVANHPYILVDDVLTAFQQLAAYYLEKTAVDV
                     FAVTGSNGKTTTKDMLAHLLSTTYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASNSLLLAP
                     ADSIVEDYLPTDKKVVRFGQGAELEITDLVERKDSLTFKANFLEQALDLPVTGKYNAT
                     NAMIASYVALQEGVSEEQIHQAFQGLELTRNRTEWKKAANGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGDQSIQLHNQMILSLSPDVLDTVIFYGEDIAE
                     LAQLVSQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLGAYDQILLKGSNSMNLAKLVE
                     RLENEVK"
     gene            5545..6249
                     /locus_tag="VVE42_001006"
     CDS             5545..6249
                     /locus_tag="VVE42_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEU3073214.1"
                     /translation="MNLWDIFFTTQATEPPKFDLFWYVSLFTLLALTFYTAHRYREKK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQSKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPFPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIAKEA"
     gene            6468..7841
                     /gene="ftsA"
                     /locus_tag="VVE42_001007"
     CDS             6468..7841
                     /gene="ftsA"
                     /locus_tag="VVE42_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEU3073215.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKD
                     GIIVDIDAAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENVIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVESVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            7858..9117
                     /gene="ftsZ"
                     /locus_tag="VVE42_001008"
     CDS             7858..9117
                     /gene="ftsZ"
                     /locus_tag="VVE42_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEU3073216.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARSATN
                     YRETVKPAHSHGFDRHFDMAETVELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            9122..9793
                     /locus_tag="VVE42_001009"
     CDS             9122..9793
                     /locus_tag="VVE42_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEU3073217.1"
                     /translation="MNVKENTELVFREVAEASLSANRESGSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDVIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELARLDKIEYVGLMTMA
                     PFEASSEQLKEIFKATQDLQREIQEKQIPNMPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            9803..10342
                     /locus_tag="VVE42_001010"
     CDS             9803..10342
                     /locus_tag="VVE42_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEU3073218.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTPVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            10342..10605
                     /locus_tag="VVE42_001011"
     CDS             10342..10605
                     /locus_tag="VVE42_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEU3073219.1"
                     /translation="MIFLIRMIYNAVDIYSLILVAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            10602..11387
                     /locus_tag="VVE42_001012"
     CDS             10602..11387
                     /locus_tag="VVE42_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEU3073220.1"
                     /translation="MNKGIYQHFSIEDRPFLDKGMEWIKKVEDSYAPFLTPFINPHQE
                     KLLKILAKTYGLACSSSGEFVSSEYVRVLLYPDYFQPEFSDFEISLQEIVYSNKFEHL
                     THAKILGTVINQLGIERKLFGDILVDEERAQIMINQQFLLLFQDGLKKIGRIPVSLEE
                     RPFTEKIDKLEQYRELDLSVSSFRLDVLLSNVLKLSRNQANQLIEKKLVQVNYHVVDK
                     SDYTVQVGDLISVRKFGRLRLLQDKGQTKKEKKKITVQLLLSK"
     gene            11396..12184
                     /gene="divIVA"
                     /locus_tag="VVE42_001013"
     CDS             11396..12184
                     /gene="divIVA"
                     /locus_tag="VVE42_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HEU3073221.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVRDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DILRPTATYLQTSDEAFKEVVSEVLGEPIPAPIEEEPIDMTRQFSQAEMEELQARIEV
                     ADKELSEFEAQIKQEVETPTPVVSPQVEEEPLLIQLAQCMKNQK"
     gene            12532..15324
                     /locus_tag="VVE42_001014"
     CDS             12532..15324
                     /locus_tag="VVE42_001014"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEU3073222.1"
                     /translation="MKLKDTLNLGKTEFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     QGKPHFTLHDGPPYANGNIHVGHAMNKISKDIIVRSKSMSGFYAPFIPGWDTHGLPIE
                     QVLSKQGVKRKEMDLVEYLKLCREYALSQVDKQREDFKRLGVSGDWENPYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GVLDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPAGEARKFVVAAELLTSLSEK
                     FGWADVQVLETYRGQELNHIVTEHPWDTAVEELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANNLEVAVTVDERGIMMKNAGPEFEGQFYEKVVPTVIEKLGNLLLAQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDEIEKVKFHSEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMVAETIEHVAQLFEEHGSSIWWERDAKDLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVVVNRPELTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFALDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPAQDTVAYDELRSVDKYMT
                     IRFNQLVKTIRDAYADFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFETEDFVQLSELPEAQTFANQEEILD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SELTIVEGPAPEAALSFEDVAFTVERATGEVCDRCRRIDPTTAERSYQAVICDHCASI
                     VEENFAEAVAEGFEEK"
BASE COUNT         4648 a         2889 c         3517 g         4482 t
ORIGIN      
        1 taaaaacgca tctctgtttt aaaaacgaaa aaatcgaaaa agcttctctc ttttccataa
       61 ttttctactc aaattgtggt acaattaaga gtaagatttt aagttagaaa tgagactgat
      121 ttgtatgaga aaatttaaca gccattcgat tccgattcgg cttaatttat tgttttcaat
      181 cgtcatttta ctctttatga ccattattgg tcgtttgttg tatatgcagg ttttgaacaa
      241 ggatttttac gaaaaaaagc tagcttcagc tagtcagacc aagattacaa gcagttcagc
      301 ccgtggggaa atttatgatg ctagtggaaa acctttggta gaaaatacgt taaagcaggt
      361 tgtttccttt acgcgtagca ataaaatgac ggctacagac ttaaaagaaa cagctaaaaa
      421 gttactgact tatgtgagca tcagttctcc aaatttgaca gaacgccagc tggcggatta
      481 ctatttggct gatcctgaaa tctataaaaa aatagtggaa gctctcccaa gtgagaaacg
      541 cttggattca gatggcaatc gtctatccga atcagaactg tataacaatg cggtcgatag
      601 tgtacaaacg agtcaactaa actatacaga ggatgaaaag aaagaaatct atctttttag
      661 tcagttaaat gctgttggaa actttgcgac aggaaccatt gcgacagatc ctctaaatga
      721 ttctcaggtg gctgttattg cctctatttc aaaggagatg cctggcatta gtatttctac
      781 ttcttgggat agaaaggttt tggaaacttc cctttcttct atagttggga gtgtatccag
      841 tgaaaaagct ggtctcccag cggaagaagc agaagcctat cttaaaaaag gctattctct
      901 aaatgatcgt gtaggaacct cctatttgga aaagcaatat gaagagacct tacaaggaaa
      961 acgctcggta aaagaaatcc atctggataa atatggcaat atggaaagcg tggatacaat
     1021 tgaggaaggt agtaagggaa acaatatcaa actgaccatt gatttggcct tccaagatag
     1081 cgtggatgct ttactgaaaa gttatttcaa ttccgagcta gaaaatggtg gagccaagta
     1141 ttctgaaggt gtctatgcag tcgcccttaa cccaaaaaca ggtgcggttt tgtctatgtc
     1201 agggattaaa catgacttga aaacgggaga gttgacgcct gattccttgg gaacggtaac
     1261 caatgtcttt gttccaggtt cggttgtcaa ggcggcgacc atcagctcag gttgggaaaa
     1321 tggagtcttg tcaggaaacc agaccttgac agaccagtcc attgtcttcc aaggttcagc
     1381 tcccatcaat tcttggtata ctcaggctta cggttcattc cctatcacag cggtccaagc
     1441 tctggagtat tcatcaaata cctatatggt ccaaacagcc ttaggtctta tggggcaaac
     1501 ctatcaaccc aatatgtttg tcggcaccag caatctagag tctgctatgg agaaactgcg
     1561 ttcaaccttt ggcgaatatg gcttgggtac tgcgacagga attgacctac cagatgaatc
     1621 tactggattt gttcccaaag agtatagctt tgctaattac attactaatg cctttgggca
     1681 gtttgataac tatacgccga tgcagttggc tcagtatgta gcaactattg caaataatgg
     1741 tgttcgtgtg gctcctcgta ttgttgaagg catttatggt aataatgata agggaggact
     1801 gggtgacttg attcagcaac tgcaaccgac agagatgaat aaggtcaata tatccgactc
     1861 cgatatgagc atcttgcacc aaggttttta tcaggttgcc catggtacta gtggattgac
     1921 aactggacgt gccttttcaa atggtgcctt ggtatccatt agcggaaaaa caggtacagc
     1981 cgaaagctat gtggcagatg gtcagcaagc aaccaatacc aatgcggtgg cctatgcccc
     2041 atctgataat ccccaaatcg ctgtcgcagt ggtctttcct cataatacca atctaacaaa
     2101 tggtgtagga ccttccattg cgcgtgacat tatcaatctg tatcaaaaat accatccaat
     2161 gaactagaaa ggaaattatg ctttatccaa cacctattgc caagttgatt gacagttatt
     2221 ctaagttacc aggtatcggg attaagacgg ctacgcgtct ggccttttat acgattggga
     2281 tgtctgctga tgatgtcaat gaatttgcaa aaaatctcct ttctgctaag agagaattga
     2341 catattgttc tatttgtgga cgtttgacag acgacgatcc ttgttctatc tgtactgatc
     2401 cgactcgtga ccagacaaca attttagttc ttgaggatag tagagatgtg gcagccatgg
     2461 aaaatatcca agaataccat ggactctatc atgtcctgca tggcctcatt tctcctatga
     2521 atggtatcag tccggacgat atcaatctca agagccttat gactcgtctt atggatagtg
     2581 aggtttcaga agtgattgtg gcgactaatg ctacagcgga tggtgaagcg acttccatgt
     2641 atctttcacg tttgctcaag ccggctggta tcaaggttac gcgtctagca cgaggtctcg
     2701 ctgtgggagc ggacattgag tatgcggacg aagtgacact cttacgagcc attgaaaatc
     2761 ggacagagtt gtaagtgtag gcaaatttac gaactccctt catttataaa aaatcaaaga
     2821 ggctgaaaat cgttcctatc ggcctctttt tgtatagtgt gatgagtagg atcaggttca
     2881 agttttaaaa aaccaagcaa atatgatata ctaaagagcg agtattctag tagaattagg
     2941 acaaataata tgaaacaaac gattattctt ttatatggtg gacggagtgc ggaacgcgaa
     3001 gtctctgtcc tttcagctga gagtgtcatg cgtgcggtca attacgaccg tttcacagtc
     3061 aagactttct ttatcagtca gtcaggtgac tttatcaaaa cacaggaatt tagtcatgct
     3121 ccggggcaag aagaccgtct catgaccaat gaaaccattg attgggataa gaaagttgca
     3181 ccaagtgcta tctacgaaga aggtgcagtg gtctttccag tccttcacgg gccaatggga
     3241 gaagatggct ctgttcaagg attcttggaa gttttgaaaa tgccttacgt tggttgcaac
     3301 attttgtcat caagtcttgc catggataaa atcacgacta agcgtgttct ggaatctgct
     3361 ggtattgccc aagttcctta tgtggctatc gttgaaggcg atgatgtgac tgctaaaatc
     3421 gctgaagtgg aagaaaaatt ggcttatcca gtcttcacta agccgtcaaa catggggtct
     3481 agtgtcggta tttctaagtc tgaaaaccaa gaagaactcc gtcaagcctt aaaacttgcc
     3541 ttccgatatg acagccgtgt cttggttgag caaggagtga atgcccgtga aattgaggtt
     3601 ggcctcttgg gtaactacga tgtcaagagc acgctacctg gagaagttgt caaggacgtt
     3661 gccttttatg actacgatgc caagtatatt gataacaaga ttactatgga tattcctgcc
     3721 aaaatcagtg atgatgtggt ggctgtcatg cgtcaaaatg cagaaacagc cttccgtgcc
     3781 attggtggcc ttggtctatc tcgttgcgat ttcttctata cagataaggg agagattttt
     3841 ctcaacgagc tcaataccat gccaggtttc acccagtggt ctatgtaccc actactttgg
     3901 gacaatatgg ggatcagcta cccagaacta atcgagcgtt tggttgacct tgccaaggaa
     3961 agttttgaca agcgcgaagc gcatttgata taaaatgaaa gagagggtag aagccagaac
     4021 catcactgca aggtgactag agttctcgga cttcagccct ttttaaagga gtagaaatga
     4081 aattaacaat ccatgaaatt gcccaagttg ttggagccaa aaatgatatc agtatctttg
     4141 aggacaccca gttagaaaaa gctgagtttg atagtcgttt gattggaact ggagatttat
     4201 ttgtgccact taaaggtgcg cgtgacggtc atgactttat cgaaagagct tttgaaaatg
     4261 gtgcggcagc aaccctgtct gagaaagagg ttgcaaatca tccctacatt ctagtagatg
     4321 atgttttgac tgcctttcaa caactagctg cctactatct tgaaaaaaca gctgttgatg
     4381 tctttgcggt tacgggttca aatggcaaga caacgaccaa ggatatgtta gcgcatttac
     4441 tatcaacaac ctacaagacc tacaaaacac aaggaaacta caataacgag attggtcttc
     4501 catacacagt tctccatatg cctgaaggga cagaaaagtt ggtcttggag atggggcagg
     4561 accacttggg cgatattcat ctcttgtctg aattggctcg tccaaaaaca gccatcgtga
     4621 ccttggttgg agaagcccat ttggcctttt tcaaagatcg ttcggaaatc gctaaaggga
     4681 aaatgcaaat tgcagatggc atggcttcga attccttgct cttggcgcca gcagattcga
     4741 ttgtagagga ctacttgcca actgataaaa aggtggtccg ttttgggcaa ggggcagagt
     4801 tggaaatcac agacttggtt gagcgtaagg acagtctgac ctttaaggct aatttcttgg
     4861 aacaagccct tgatttgcca gtgactggta agtacaatgc taccaatgct atgattgcat
     4921 cctatgttgc cctacaagaa ggagtttcag aggagcaaat tcatcaggcc ttccaaggtc
     4981 ttgaattgac gcgtaatcgt actgagtgga agaaagcagc caatggagca gatatcctgt
     5041 cagacgtata taatgccaat ccaactgcta tgaagctgat tttagagact ttctctgcca
     5101 ttccagccaa tgaaggtggc aagaaaattg cagtgttggc ggatatgaag gagcttggtg
     5161 accagtctat tcaactccat aaccagatga ttttgagcct ttctccagat gtgcttgata
     5221 ccgttatttt ctatggagaa gacattgctg aattagccca attggtcagt caaatgttcc
     5281 caattggcca cgtttactac ttcaagaaaa cagaagacca ggatcaattt gaagacctag
     5341 tcaagcaggt taaggaaagc cttggagcct atgaccaaat cttgctaaaa ggttctaact
     5401 ctatgaatct agccaaattg gtagaacgtt tagaaaatga agtcaagtga ttttgtcaaa
     5461 catttacgta tttctaaatc catttttaac aactagcatg gtataataaa atgcaggaaa
     5521 attttgaatc atgaggaaga ctagatgaat ttatgggata ttttctttac gactcaggca
     5581 accgagccgc ccaaatttga ccttttttgg tatgttagcc tatttacgct cttagcctta
     5641 accttttata cagcccatcg ctatcgtgaa aagaaggttt accaacgatt tttccaaatc
     5701 ttgcagactg ttcagttaat ccttctttat ggttggtact gggtcaatca tatgccactg
     5761 tcagaaagcc taccctttta ccattgccgt atggctatgt ttgtggtact cttgcttcct
     5821 ggtcagtcca aatataaaca atactttgca ttattgggaa catttgggac attagcagcc
     5881 tttgtttatc cagtgccaga tgcttaccct tttccacata tcaccattct atcctttatc
     5941 tttggtcatt tagcactctt ggggaactct ctagtttatc tattgagaca gtataatgcg
     6001 cgattgctgg atgtgaaggg aatttttctc atgacctttg ccctaaatgc cttgattttt
     6061 gtggtcaatt tggtgacagg tggcgattac ggatttttga caaaaccgcc attggttggg
     6121 gatcacggtc tagtagctaa ttatttactt gtttcaattg tgctggtagc tactatcagt
     6181 ttgactaaga aaatcttaga attcttttta gctcaagaag cagaaaaaat gattgcaaag
     6241 gaagcttaac acagagcttt cttttttgct cttagagagt ttttacaagc agcttataaa
     6301 ataagaattt ctgaatagac aaactcaaaa aatggctggg aaatttagga aaaaagcaag
     6361 cacgattaaa ttttttgtgt tataatattt tgtgaatagc tatgcctatg tttagctatg
     6421 gaataatacg aagtgcgaaa cttggaagat agagaggaag cgatgtaatg gctagagaag
     6481 gcttttttac aggtctagat attggaacaa gctctgtcaa ggtgcttgtg gccgagcaga
     6541 gaaatggtga attaaatgta attggcgtga gtaatgccaa aagtaaaggt gtaaaggatg
     6601 gaattattgt tgatattgat gcagcagcaa ctgctatcaa gtcagccatt tcccaagcgg
     6661 aagaaaaggc aggcatttcg attaaatcag taaatgtcgg cttgcctggt aatcttttgc
     6721 aggtagaacc aactcagggg atgattccag taacatctga tactaaggaa attacggatc
     6781 aagatgttga aaatgttgtc aaatcagctt tgacaaagag tatgacacct gaccgtgaag
     6841 tcattacctt tattcctgaa gaatttattg tggatggttt ccaagggatt cgtgacccac
     6901 gtggcatgat gggggttcgc cttgaaatgc gtggtttgct ttatacagga cctcgtacta
     6961 tcttgcacaa tttgcgtaag acggttgagc gtgcaggtgt tcaggttgaa aatgttatca
     7021 tttcaccact agcaatggtt cagtctgttt tgaacgaagg ggaacgtgaa tttggtgcta
     7081 cagtgattga tatgggggca ggtcaaacga ctgtcgctac aatccgtaat caagaactcc
     7141 agttcacaca tattctccaa gaaggtggag attatgtaac taaagatatc tccaaggttt
     7201 tgaaaacctc tcgcaaatta gcggaaggct tgaaactgaa ttacggggaa gcctatccgc
     7261 ctcttgcaag caaagaaacc ttccaagtag aggttattgg agaagtggaa gcagtcgaag
     7321 tgacggaagc ctacttgtca gaaattattt ctgcacgaat caagcacatc cttgaacaaa
     7381 tcaagcaaga attagataga aggcgtctat tggacctccc tggtggtatt gtcttaatcg
     7441 gtgggaatgc cattttacca ggtatggttg agcttgctca ggaagtcttt ggcgtccgtg
     7501 tcaagcttta tgttccaaat caagttggta tccgtaatcc agcctttgcg catgtgatta
     7561 gtttatcaga atttgcgggt caattaacag aagttaatct tttggctcag ggagcgataa
     7621 aaggtgagaa tgacttaagt catcagccaa ttagttttgg tgggatgctg caaaaaacag
     7681 ctcagtttgt acaatcaacg cctgttcaac cagctcctgc tccagaagta gagtcggtgg
     7741 cgcctacaga accaatggcg gatttccaac aagcttcaca aaataaaccg aaattagcag
     7801 atcgtttccg tggcttgatc ggaagcatgt ttgacgaata aagaggaaaa ataaattatg
     7861 acattttcat ttgatacagc tgctgctcaa ggggcagtga ttaaagtaat tggtgtcggt
     7921 ggaggtggtg gcaatgccat caaccgtatg gtcgacgaag gtgttacagg cgtagaattt
     7981 atcgcagcaa acacagatgt acaagcattg agtagtacaa aagctgagac tgttattcag
     8041 ttgggaccta aattgactcg tggtttgggt gcaggaggtc aacctgaggt tggtcgtaaa
     8101 gccgctgaag aaagcgaaga aacactgacg gaagctatta gtggtgccga tatggtcttc
     8161 atcactgctg gtatgggagg aggctctgga actggagctg ctcctgttat tgctcgtatc
     8221 gccaaagatt taggtgcgct tacagttggt gttgtaacac gtccctttgg ttttgaagga
     8281 agtaagcgtg gacaatttgc tgtagaagga atcaatcaac ttcgtgagca tgtagacact
     8341 ctattgatta tctcaaacaa caatttgctt gaaattgttg ataagaaaac accgcttttg
     8401 gaggctctta gcgaagcgga taacgttctt cgtcaaggtg ttcaagggat taccgatttg
     8461 attaccaatc caggattgat taaccttgac tttgccgatg tgaaaacggt aatggcaaac
     8521 aaagggaatg ctcttatggg tattggtatc ggtagtggag aagaacgtgt ggtagaagcg
     8581 gcacgtaagg caatctattc accacttctt gaaacaacta ttgacggtgc tgaggatgtt
     8641 atcgtcaacg ttactggtgg tcttgactta accttgattg aggcagaaga ggcttcacaa
     8701 attgtgaacc aggcagcagg tcaaggagtg aacatctggc tcggtacttc aattgatgaa
     8761 agtatgcgtg atgaaattcg tgtaacagtt gttgcaacgg gtgttcgtca agaccgcgta
     8821 gaaaaggttg tggctccaca agctagatct gctactaact accgtgagac agtgaaacca
     8881 gctcattcac atggctttga tcgtcatttt gatatggcag aaacagttga attgccaaaa
     8941 caaaatccac gtcgtttgga accaactcag gcatctgctt ttggtgattg ggatcttcgc
     9001 cgtgaatcga ttgttcgtac aacagattca gtcgtttctc cagtcgagcg ctttgaagcc
     9061 ccaatttcac aagatgaaga tgaattggat acacctccat ttttcaaaaa tcgttaagta
     9121 aatgaatgta aaagaaaata cagaacttgt ttttcgagaa gttgcagagg ctagtctgag
     9181 tgctaatcga gagagtggtt cggtctctgt cattgcagtt accaagtatg tagatgtacc
     9241 gacagcggaa gccttgcttc cgctaggtgt tcatcatatc ggtgaaaatc gtgtagataa
     9301 gtttctggaa aaatatgaag ctttaaaaga tcgagatgtg acttggcatt tgattggtac
     9361 cttgcaaaga cgtaaggtga aagatgtcat tcaatacgtt gattatttcc atgcattgga
     9421 ctcagtaaag ctagcagggg aaattcaaaa aagaagtgac cgagtcatca agtgtttcct
     9481 tcaagtaaat atttctaaag aagaaagcaa acacggtttt tcgagagagg aactgctgga
     9541 aatcttgcca gagttagcca gactagataa gattgaatat gttggtttaa tgacgatggc
     9601 accttttgag gctagcagtg agcagttgaa agagattttc aaggcgaccc aagatttaca
     9661 aagagaaatt caagagaaac aaattccaaa tatgcctatg accgagttaa gtatgggaat
     9721 gagtcgtgat tataaagaag cgattcaatt cggttccact tttgttcgta taggtacatc
     9781 attttttaag taggagagaa ccatgtcttt aaaagataga ttcgatagat ttatagatta
     9841 ttttacggag gatgaggatt caagtctccc ttatgaaaaa agagatgagc ctgtgtttac
     9901 tccagtaaat tcttcacagg aaccggctct cccaatgaat caaccttcac agtcggctgg
     9961 tacaaaagag aacaatatca ccagacttca tgcaagacaa caggaattgg caaatcagag
    10021 tcagcgtgca acggataagg tcattataga tgttcgttat cctagaaaat atgaggatgc
    10081 aacagaaatt gttgatttat tggcaggaaa cgaaagtatc ttgattgatt ttcagtatat
    10141 gacagaggtg caggctcgtc gttgtttgga ctatttggat ggagcttgtc atgttttagc
    10201 tggaaatttg aaaaaggtag cttctaccat gtatttgttg acaccagtga atgttattgt
    10261 aaatgttgaa gatatccgtt taccagatga agatcaacag ggtgagttcg gttttgatat
    10321 gaagcgaaat agagtacgat aatgattttt ttaattcgta tgatttataa tgcagtggat
    10381 atttactccc tgattttggt agccttcgct gtcatgtctt ggtttccagg tgcctacgaa
    10441 tccagtttag gtcgttggat tgtagcgttg gtgaaaccag tgcttgctcc cttgcaacgc
    10501 ctgcctttac agatagcggg tcttgattta tctgtttggg tcgcgattgt tttggttcga
    10561 tttttaggag aaaacctagt gcgttttctg gcgatgatag gatgaataaa gggatttatc
    10621 agcatttctc catagaagat cgtccatttc ttgacaaggg aatggaatgg ataaagaagg
    10681 tagaagatag ctatgctcct tttttaactc cttttatcaa tcctcatcag gagaagctat
    10741 taaagatttt ggccaaaacc tatggtcttg cttgtagcag tagtggggaa ttcgtctcga
    10801 gtgagtatgt tcgagtttta ttatacccag attatttcca accagagttt tcagattttg
    10861 aaatatctct ccaggaaatt gtgtattcca ataaatttga acatttaacg catgctaaga
    10921 ttttagggac agtcatcaat caattaggga ttgaacggaa actttttgga gatatcctag
    10981 tagatgaaga acgggcgcag attatgatta atcagcagtt tcttcttctc tttcaagatg
    11041 gactaaagaa aattggtcgt atacctgttt cgctggagga acgtcctttc accgagaaaa
    11101 tagataagct agaacagtat cgagaactgg atttatctgt gtctagtttt cgattagatg
    11161 ttcttttatc aaatgttttg aaactatcta ggaatcaagc aaaccagttg attgaaaaga
    11221 aacttgtcca agtaaattat catgtggtag acaaatcaga ttacactgtt caagttggag
    11281 acttgattag tgtgagaaaa tttggtcgct tgagattact tcaagataag ggacaaacga
    11341 aaaaagagaa gaaaaaaata accgtccagt tattattaag taagtgagga atagaatgcc
    11401 aattacatca ttagaaataa aggacaagac ttttggaact cgattcagag gttttgatcc
    11461 agaagaagtc gatgaatttt tagatattgt ggttcgtgat tacgaagatc ttgtgcgtgc
    11521 gaatcatgat aaaaatttgc gtattaagag tttagaagag cgtttgtctt actttgatga
    11581 aataaaagat tcattgagcc agtctgtatt gattgctcag gatacagctg agagagtgaa
    11641 acaggcggcg catgaacgtt caaacaatat cattcatcaa gcagagcaag atgcgcaacg
    11701 cttgttggaa gaagctaaat ataaggcaaa cgagattctt cgtcaagcaa ctgataatgc
    11761 taagaaagtc gctgttgaaa cagaagaatt gaagaacaag agccgtgtct tccaccaacg
    11821 tctcaaatct acaattgaga gtcagttggc tattgttgaa tcttcagatt gggaagatat
    11881 tctccgtcca acagctactt atcttcaaac cagtgatgaa gcctttaaag aagtggttag
    11941 cgaagtactt ggagaaccga ttccagctcc aattgaagaa gaaccaattg atatgacacg
    12001 tcagttctct caagcagaaa tggaagaatt acaagctcgt attgaggtag ccgataaaga
    12061 attgtctgaa tttgaagctc agattaaaca ggaagtggaa actccaactc ctgtagtgag
    12121 tcctcaagtt gaagaagagc ctctgctcat ccagttggcc caatgtatga agaaccagaa
    12181 gtagctccag tgcatccgac aggtccaaca ccagctacag aaactgttga ttcagcaccg
    12241 ggatttgaag caccgcaaga atctgttaca attttataag aaatattctg agaacaatat
    12301 cttatcctta tattcccagc gagcaggaga tggtgtgagt cctgcattcc ctatcgataa
    12361 gattatcctc tcaaaaactc aagtctgaat ctagtaagat ttgacgttcc ccacgttacg
    12421 ggataagagg gagaaagact aaaacttttt ccgaataaag gtggtaccac gattttcgtc
    12481 ctttttggaa gtcgtggttt ttaatttgtt attatttata aaggagatac catgaaactc
    12541 aaagacaccc ttaatcttgg gaaaactgaa ttcccaatgc gtgcaggcct tcctaccaaa
    12601 gagccagttt ggcaaaagga atgggaagat gcaaaacttt atcaacgtcg tcaagaattg
    12661 aaccaaggaa aacctcattt caccttgcat gatggccctc catacgctaa cggaaatatc
    12721 cacgttggac atgctatgaa caagatttca aaagatatca ttgttcgttc taagtctatg
    12781 tcaggatttt acgcaccatt tattcctggt tgggatactc atggtctgcc aatcgagcaa
    12841 gtcttgtcaa aacaaggtgt caaacgtaaa gaaatggact tggttgagta cttgaaactt
    12901 tgccgtgagt acgctctttc tcaagtagat aaacaacgtg aagattttaa acgtttgggt
    12961 gtttctggtg actgggaaaa tccatatgtg accttgactc ctgactatga agcagctcaa
    13021 attcgtgtat ttggtgagat ggctaataag ggttatatct accgtggtgc taagccagtt
    13081 tactggtcat ggtcatctga gtcagccctt gctgaagcag agattgaata ccatgacttg
    13141 gtttcaactt ccctttacta tgccaacaag gtaaaagatg gcaaaggagt tctagataca
    13201 gatacttata tcgttgtctg gacaacgact ccatttacta tcacagcctc tcgtggtttg
    13261 acggttggtg cagatattga ttacgttttg gttcaacctg ctggtgaagc tcgtaagttt
    13321 gtcgttgctg ctgaattatt gactagcttg tctgagaaat ttggctgggc tgatgttcaa
    13381 gttttggaaa cttaccgtgg ccaagaactc aaccacatcg taacagaaca cccatgggat
    13441 acagctgtag aagagttggt aattcttggt gaccacgtta cgactgactc tggtacaggt
    13501 attgtccata cagcccctgg ttttggtgag gacgattaca atgttggtat tgctaataat
    13561 cttgaagtcg cagtgactgt tgatgaacgt ggtatcatga tgaagaatgc tggtcctgaa
    13621 tttgaaggtc aattctatga aaaggtagtt ccaactgtta ttgaaaaact tggtaacctc
    13681 cttcttgccc aagaagaaat ctctcactca tatccatttg actggcgtac taagaaacca
    13741 atcatctggc gtgcagttcc acaatggttt gcctcagttt ctaaattccg tcaagaaatc
    13801 ttggacgaaa ttgaaaaagt gaaattccac tcagaatggg gtaaagtccg tctttacaat
    13861 atgatccgtg accgtggtga ctgggttatc tctcgtcaac gtgcttgggg tgttccactt
    13921 cctatcttct acgctgaaga tggtacagct atcatggtag ctgaaactat tgaacacgta
    13981 gctcaacttt ttgaagaaca tggttcaagc atttggtggg aacgtgatgc caaagacctc
    14041 ttgccagaag gatttactca tccaggttca ccaaacggcg agttcaaaaa agaaactgat
    14101 atcatggacg tttggtttga ctcaggttca tcatggaatg gagtggtggt aaaccgtcct
    14161 gaattgactt acccagccga cctttaccta gaaggttctg accaataccg tggttggttt
    14221 aactcatcac ttatcacatc tgttgccaac catggcgtag caccttacaa acaaatcttg
    14281 tcacaaggtt ttgcccttga tggtaaaggt gagaagatgt ctaaatctct tggaaatacc
    14341 attgctccaa gcgatgttga aaaacaattc ggtgctgaaa tcttgcgtct ctgggtaaca
    14401 agtgttgact caagcaatga cgtgcgtatc tctatggata tcttgagcca agtgtctgaa
    14461 acttaccgta agattcgtaa cactcttcgt ttcttgattg ccaatacatc tgactttaac
    14521 ccagctcaag atacagtcgc ttacgatgag cttcgttcag ttgataagta catgacgatt
    14581 cgctttaacc agcttgttaa gaccattcgt gatgcctatg cagactttga attcttgacg
    14641 atctacaagg ccttggtgaa ctttatcaac gttgacttgt cagccttcta ccttgatttt
    14701 gccaaagatg ttgtttacat tgaaggtgcc aaatcactgg aacgccgtca aatgcagact
    14761 gtcttctatg acattcttgt caaaatcacc aaactcttga caccaatcct tcctcacact
    14821 gcggaagaaa tttggtcata tcttgagttt gaaacagaag acttcgtcca attgtcagag
    14881 ttaccagagg ctcaaacttt tgctaatcaa gaagaaatct tggatacatg ggcagccttc
    14941 atggacttcc gtggacaagc acaaaaagcc ttggaagaag ctcgtaatgc aaaagttatc
    15001 ggtaaatcac ttgaagcaca cttgacagtt tatccaaatg aagttgtgaa aactctactc
    15061 gaagcagtaa acagcaatgt agctcaactt ttgatcgtgt ctgagttgac catcgtagaa
    15121 ggaccagctc cggaagctgc ccttagcttc gaagatgtag ccttcacagt tgaacgtgct
    15181 actggtgaag tatgtgaccg ttgccgtcgt atcgacccaa caacagcaga acgcagctac
    15241 caggcagtta tctgtgacca ctgtgcaagc atcgtagaag aaaactttgc ggaagcagtc
    15301 gcagaaggat ttgaagagaa ataagattga aaagtctagg caaaattcaa tttgagaaga
    15361 aaagacaact aattttatag tctattaaac gcattgtatc acgtttttga atacctgata
    15421 tgatgcgttt tttatttatt ttaaaaattt gcgaggtatg actttttata ctcaacaaga
    15481 atcaaagaga aacttagcaa tctaacagta gtaagataaa ataggaattt gatatt
//
LOCUS       DAVGJL010000041        14953 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.041, whole genome shotgun sequence.
ACCESSION   DAVGJL010000041 DAVGJL010000000
VERSION     DAVGJL010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14953
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.041"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..182
                     /locus_tag="VVE42_001015"
     CDS             <1..182
                     /locus_tag="VVE42_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001092275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073223.1"
                     /translation="FYKEHQVKPFISPERDLDTWLLSPKPVPKRNMDLLADDSLAGDI
                     ILLWRIQFGTFTTET"
     gene            661..873
                     /locus_tag="VVE42_001016"
     CDS             661..873
                     /locus_tag="VVE42_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073224.1"
                     /translation="MSFYGLFYNGIAITPNTYLSAWFVNFIAALPLNFLIVEPIARFI
                     LSSFQKPFTGEEVEDFQDDDEIPTII"
     gene            957..1760
                     /locus_tag="VVE42_001017"
     CDS             957..1760
                     /locus_tag="VVE42_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU3073225.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQNSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIGGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS"
     gene            complement(1816..3357)
                     /locus_tag="VVE42_001018"
     CDS             complement(1816..3357)
                     /locus_tag="VVE42_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU3073226.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMKMAEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DRPLTKPLNLTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKEYRGSILMVCHEPDFYEGWIDQIWD
                     FNNLT"
     gene            complement(3359..3841)
                     /locus_tag="VVE42_001019"
     CDS             complement(3359..3841)
                     /locus_tag="VVE42_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEU3073227.1"
                     /translation="MMKKTYNHILVWGVIFYSICIVYFCFTPQEQSTVGVGTPGIQHL
                     GRLVFLLTPFNSLWKLGEVSDIGQLCWIFLQNILNVFLFFPLIFQLLYLFPNLRKTKK
                     VLLFSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKIRN
                     "
     gene            complement(3841..4926)
                     /gene="rlmN"
                     /locus_tag="VVE42_001020"
     CDS             complement(3841..4926)
                     /gene="rlmN"
                     /locus_tag="VVE42_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEU3073228.1"
                     /translation="MKPSIHSLTHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQRIVQESADGTVKYLFELPDGMLIETVLMRQHYGLS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFAERGQDERVSHIVVM
                     GIGEPFDNYNNVLNFFRTINDDKGMAIGARHIMISTSGLAHKIRDFADEGVQVNLAVS
                     LHAPNNELRSSIMKINRAFPIEKLFAAIEYYIETTNRRVTFEYIMLNEVNDGVEQALE
                     LAELLKNIKKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLKKKGVNCVVRQEHGT
                     DIDAACGQLRSNTMKRDRQKAVAAVNP"
     gene            complement(4947..5477)
                     /locus_tag="VVE42_001021"
     CDS             complement(4947..5477)
                     /locus_tag="VVE42_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEU3073229.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISRLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDQH
                     SQKEDKEQRHFVIRQK"
     gene            complement(5634..6239)
                     /locus_tag="VVE42_001022"
     CDS             complement(5634..6239)
                     /locus_tag="VVE42_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEU3073230.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELAAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            complement(6412..7449)
                     /locus_tag="VVE42_001023"
     CDS             complement(6412..7449)
                     /locus_tag="VVE42_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEU3073231.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQVLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNPSPTTKLIIFAEGKLDSKRRLVKLLKRDAKVFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKADSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            complement(7483..8418)
                     /locus_tag="VVE42_001024"
     CDS             complement(7483..8418)
                     /locus_tag="VVE42_001024"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEU3073232.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFAYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
     gene            complement(8971..10161)
                     /locus_tag="VVE42_001025"
     CDS             complement(8971..10161)
                     /locus_tag="VVE42_001025"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEU3073233.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
                     AELRKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVIDK
                     VIKEVIPSSYLDDKTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAADLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
     gene            complement(10376..11719)
                     /locus_tag="VVE42_001026"
     CDS             complement(10376..11719)
                     /locus_tag="VVE42_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU3073234.1"
                     /translation="MSFTKFQFKNYIREALEELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKARQIEKLASNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLETVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDKNAQIYQLTQLMQPYLAMIFVNTKTRADELHSYLTAQGLKVAKIHGDI
                     APRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLGIKFSPKMVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
     gene            complement(11859..12674)
                     /locus_tag="VVE42_001027"
     CDS             complement(11859..12674)
                     /locus_tag="VVE42_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU3073235.1"
                     /translation="MKFLTLNTHSWMEKEAEEKFQILLEDILEKDYDLICFQEINQEM
                     TSSEVEVNDLYQALPAAEPIHQDHYVRLLVEKLSEQGKNYYWTWAYNHIGYDRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVALAETVVDGKELAVASVHLSWWDKGFQEE
                     WARFEAVLKKLNKPLLLAGDFNNPAGQEGYQAILASSLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAMLNWK"
     gene            complement(12726..14906)
                     /locus_tag="VVE42_001028"
     CDS             complement(12726..14906)
                     /locus_tag="VVE42_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEU3073236.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPTFTPLVITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTFFGGSIKVADYFISVLEAPALNMGVFVGIISGFVGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSAIAAILLAAFWPVVQTGINNFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYDILTGAAKGTQVFG
                     QDPLWLAWVTDLVNLKGTDASQYQHLLDTVHPARFKVGQMIGSFGILMGVIVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFIATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEETSSEVKVAAGSQAVNIINLLGGRVNIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADILKSDIQDILDSGEIIPETLPSQMTEA
                     QQNTVHFKDLTEEVYSVADGQVVGLEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHAFGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVAAGDLLVTADL
                     DAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
BASE COUNT         4455 a         3278 c         2772 g         4448 t
ORIGIN      
        1 aattttataa agaacaccaa gtcaaaccct tcatatcgcc tgaacgagat ttggatactt
       61 ggctcctaag tcccaagccc gttcccaagc gaaatatgga cttattagca gatgattcac
      121 tagcaggaga tatcatttta ctatggagaa tccagtttgg tacttttacc actgaaacat
      181 gattttaaaa actcccattc gaatatgatt caaaattcgc ttagaatcaa tgttttccca
      241 ctttttcaaa aaataaattt tcatctaatt taaataactt tcaattaagt gtgaattttg
      301 cttaaaatca taaaaaaaca ccccatggtg tgaaccatga agtgttgtaa atgctgtatt
      361 gtaactagtt cttaacgttt agagaactgg ctagctttac gagctttctt aagatctggt
      421 tccctaaata ctttgagtac acttatggca ttgatgcccc taaacatcta aaaactctgg
      481 tagaaaaagg gtatgctctt gtcgaaactt cttttgactc acttgaccat ctaaataata
      541 ccatgaaaaa gaatatcctg aagaagaagg gagttactgg actttctaaa atgaaatctt
      601 gcgacctgga tcaagccctt catgagcatt tttcagaaga agaattagct ggtcactttc
      661 atgtccttct atggactttt ttacaatggc attgctatca cacccaatac ctatctaagc
      721 gcctggttcg taaactttat tgcagctctt cctctaaatt tcctaattgt tgaaccaatt
      781 gcccgtttta tactaagttc ttttcagaaa ccatttactg gggaagaagt tgaagatttt
      841 caagatgatg atgaaatccc aactattatc taagccagtt ctgtaaacta ctaatatttg
      901 aaatccactt ccttttaggg tacaatggtt ataaatgaat ttttgagagg aacagaatga
      961 aaaaactagc aacccttctt ttactgtcta ctgtagccct agctgggtgt agcagcgtcc
     1021 aacgcagtct gcgtggtgat gattatgttg attccagtct tgctgctgaa gaaagttcca
     1081 aagtagctgc ccaatctgcc aaggagttaa acgatgcttt aacaaacgaa aacgccaatt
     1141 tcccacaact atctaaggaa gttgctgaag atgaagccga agtgattctc cacacaagcc
     1201 aaggtgatat tcgcattaaa ctcttcccta aactcgctcc tctagcggtt gaaaatttcc
     1261 tcactcacgc caaagaaggc tactataacg gtattacctt ccaccgtgtc atcgatggct
     1321 ttatggtcca aactggagat ccaaaagggg acggtacagg tggtcagtcc atctggcatg
     1381 acaaggataa gactaaagac aaaggaactg gtttcaagaa cgagattact ccttatttgt
     1441 ataacatccg cggtgctctt gctatggcta atactggtca accaaacacc aatggcagcc
     1501 agttcttcat caaccaaaac tctacagata cctcttctaa actccctaca agcaagtatc
     1561 cacagaaaat tatcgaagcc tacaaagaag gtggaaaccc tagtctagat ggcaaacacc
     1621 cagtctttgg tcaagtgatt ggcggtatgg atgttgtgga taagattgct aaggccgaaa
     1681 aagatgaaaa agacaagcca actactgcta tcacaatcga cagcatcgaa gtggtgaaag
     1741 actacgattt taaatcttaa aaaccaaaaa aatacagtat ccacattcgg tactgtattt
     1801 cttttactct cattcttaag ttaaattatt aaaatcccat atttggtcta tccagccttc
     1861 ataaaagtct ggctcgtggc agaccataag gatagatccc ctatattctt tgagagcgcg
     1921 tttgagctca tcctttgcat ccacatccaa atggttggtc ggctcgtcca gcactaaaac
     1981 gttgttttca cgattcatca agagacagaa acgaaccttg gcttgctctc cccctgataa
     2041 tacttgaatc tggctttcaa tatgtttggt tgtcaaacca caacgggcaa gggctgcacg
     2101 gacttctgct tgattaaggg caggaaaggc attccagaca gcttcaagag gagtttggcg
     2161 attaccgcct tctacttcct gctcaaaata accaagttct aaataatctc cacgctccac
     2221 ttccccagcg attggcgaga taatgcccaa gagactcttc aagagagttg tttttccaat
     2281 accattagca ccaataatcg caaccttttg attgcgttca aaggtaagat ttaaaggctt
     2341 agtaagagga cggtcgtaac caatttgcaa gttcttggct tggaagataa agcgccctgg
     2401 tgtacgagct ggtttgaaat caaaggatgg ttttggtttc tcactttgga gttcgataat
     2461 atccatctta tccaatttct tttgacgaga catagccata ttacgagttg caacacgggc
     2521 tttattacga gccacaaagt ccttgaggtc tgcaatctct ttctgctggc gttcgtaggc
     2581 tgcctctagc tgagatttct tcatagcata aacttcttgg aactggtagt agtcaccaga
     2641 gtaacgcgtc agctgttgat tttccacatg atagacaata ttaataacgt cattgaggaa
     2701 tggaatatcg tgcgaaatga gaacaaaggc attctcatag ttttggagat agcgcttgag
     2761 ccaatcaata tgctcagcat ccaagtagtt ggtcggctcg tccaacagca agatatcagg
     2821 cttttcaagg agaagttttg ccaaaagcac cttggttctt tgcccacctg acaaagaagt
     2881 tacatccgta tccatgccaa agtccataac accaagagca cgcgctactt cgtcaatctt
     2941 agcatccaag gtatagaaat cacgactctc cagacggtct tgaagttctc ctacttcttc
     3001 catgagagca tcaacatccg caccgtcttc agccattttc atatagaggt cattgatacg
     3061 agcttcagct ttgaaaagct catcaaaagc cgtacggaga acatcacgca ccgactgtct
     3121 ttcagcaagg acagagtgct gatccaagta accagccgtc acatatttgg accactcaac
     3181 ctttccttca tctggcagca ttttaccagt cacaatactc ataaaggttg attttccttc
     3241 accattggca ccgaccaggc cgatatgttc tcccttgagg agacggaagg acacatcttc
     3301 aaaaattgca cggtcaccaa aaccgtgact cagattttta acttctaaaa tactcatttt
     3361 aattccttat cttgttttta tgtaatcgtt tataaaggag ccaagccaga tagccaccca
     3421 aagtgttggt ccacaaatca tcaatctcaa agacgcgatt gaaatcaaag aaaaagtcca
     3481 agattaattg cgtacactcg attccaagac tcacaagaaa actaaaaaga aggacctttt
     3541 ttgttttccg caaatttgga aatagataaa ggagttggaa aatcagagga aaaaacaaga
     3601 agacattgag gatattttgt aaaaaaatcc aacataattg tccaatgtca ctcacttcgc
     3661 ccagtttcca gagagaattg aaaggagtca aaagaaaaac caggcgtcca agatgctgaa
     3721 tacctggagt tcccactccc acggtagatt gttcttgagg agtaaagcaa aaatagacaa
     3781 tgcaaatgct atagaaaatg actccccaga ccaaaatatg attataagtc ttcttcatca
     3841 ttaaggattt accgctgcga ctgccttctg gcggtcacgt ttcattgtgt tagagcgcaa
     3901 ttgtccacaa gctgcgtcaa tatctgtacc atgctcttga cgaaccacac agttgacccc
     3961 ttttttctta agcgtatcat agaaagccaa cacgcgctct ttgggactac ggctatattg
     4021 gtcatgctca ctaactgggt tataaggaat caagtttaca taagacaatt tcttgatgtt
     4081 cttgagcaat tcagccaatt ccaaggcttg ttctacaccg tcgttgactt cattaagcat
     4141 gatatattca aaggttacac gacggtttgt tgtctcaata tagtattcaa tagcagcaaa
     4201 gagtttttca atcggaaagg cacggttaat tttcatgata cttgaacgaa gttcattgtt
     4261 gggtgcgtga agagacacgg caagattgac ctgaacccct tcatcagcaa agtcacgaat
     4321 tttatgagcc aaacctgagg ttgaaatcat gatgtgacga gcaccgatag ccattccttt
     4381 atcatcattg atagtacgaa agaaattcaa gacattgttg taattatcaa agggctcacc
     4441 gattcccatg acaacgatat ggctgacgcg ttcatcctga ccacgctcag caaagtattt
     4501 ctgaaccagc atgatttgcg ctacgatttc accgttattg aggtcacgtt gcttcttaat
     4561 caaaccagag gcacagaagg tacaaccgat attacagccg acctgagtgg tcacacagac
     4621 agataaacca tagtgttgac gcatgagtac agtctcaatt aacataccgt cgggcaattc
     4681 aaagagatat ttgactgtac catcagcaga ctcttgcaca atacgttgtt tcaagggatt
     4741 gaccacaaac tggtcattga gcttagcaat caaatccttg gaaaggttgg tcatttcttc
     4801 aaatgactgc acacgtttac ggtagagcca ttcccagatt tgatctgcac ggaatttctt
     4861 ttctccctgc tccaataccc attcctgcat ggtttgatgt gtcaaactat gaattgaggg
     4921 tttcatttct tctccttatt ctctactcac ttctgacgaa tgacaaaatg acgttgttcc
     4981 ttgtcctctt tctgagaatg ctggtctttc tgacgacgtc tatttttctt atctgcattc
     5041 gactttcgtt tagtttgagt cggtttcttt ccttttctag aaggtgtttc ttcttccgtc
     5101 ttacgcattt tcttgtcaaa tgatgctcgc ttaggggctt cattttctaa gacaaaatag
     5161 gcacaaccat aactacaata ctctaaaagg tagtcttgta aacgactgat tttttcaagt
     5221 ttttcttctg ttcggtcatc cttgtaaaaa cctcgtaggc gaagctgttc gttgctccag
     5281 tcccccacga tataatcaaa cttggttaat acttctgaaa aacgctgatt aaaagtcgtc
     5341 acatcaaagg catccttgat attttcaacc aaggaaaaag ctatcccttc cgtttcgacc
     5401 ttgtccccgt gtaaatggaa ctccggacca ggaaacttgt tatagttgta taattcaggt
     5461 gcaatttctt ttcgcataga tatccttttt ccacgattac ttaatacttt attctaccat
     5521 aatttctagc agttagcacg tttctcataa aaatgaaaaa agtctgacga ttttgtcaga
     5581 ccagaatctt ataacctaaa aaagagaaga acaattcttc cctccaacta tcattattta
     5641 gcagctgcgt acaattcatc tactttattc cagttgatta ctgaaaagaa agctttgata
     5701 tagtcaggac gcacgttgcg gtatttcacg tagtaagcat gttcccaaac gtccaagccc
     5761 aagattggtt ttttaccttc tgagattggt gtgtcttggt ttgctgttga agtcacttca
     5821 agtttccctt ctttgttgac aaccaaccat gcccaacctg aaccaaaacg agttgttgct
     5881 gctgcagtga aggctgcttg gaattcttca aatgaaccaa atgttgcatc gattgctgct
     5941 gccagttctg ctgaaggagc tgttttctcg ggagtcatca attcccagaa aagagcgtgg
     6001 ttcaagtgtc cgccaccatt gttgataagt gcttgacgga tatcagctgg gatagattct
     6061 acatcagcaa gcaaggcttc aaggtcttca ccgatttcag ggtgtttttc taaagctgca
     6121 ttggcattgt tgacataagt ttgatggtgt ttgtcatggt gcaaatgcat tgtttccgca
     6181 tcgatgtatg gttccaaagc gtcgtatgca tatggaagtt ctggtaagat aatagccatc
     6241 tgtaatacct ctttttcttt ctatatgaaa atgataacgc aaacattcac ttttttcaag
     6301 ttttttgctt atagataagg aaatcactga catactttct aataaaataa gcaaatcagc
     6361 aagaaaccca cgacaagata atcctgacgc gggttgtagt ttcaacaaat gtcaattgac
     6421 ctgactagca atctgtaaga gtgccttttc aaaaaggaaa cctttttcat aaagacctgt
     6481 cttaatctga tagtctgtct caatcaaata ggaaatagct tgcttcagaa agctcaaaga
     6541 aagtcctctc gaatctctta atgcaaactt gatttgataa ggatttgggt tacgtcccag
     6601 ataactacct aaactacttg caatctgcga ttctgtttgg ccagactccg ccaaaatctt
     6661 cacctgagta aaagtccgaa attgtcccag catgactgca atcagtttga tttcatcttc
     6721 cccttgcaag gtcaagtctc tcaccaaatc gcgcgcctga tccatctttt tagtcagaat
     6781 aaactgagtt aaatcaaaaa tattgtcctg caaggtcttg ggaattgcgt taacaatatc
     6841 ctcttcctcg ataacagaat ccgccttata ggactgtaaa aagagaagat ttttctggat
     6901 ttcgctaaat tgaaaccccg acttgatgag gagattttca aaagaatgat tggtaaactg
     6961 cagaccttgt ttctgactcc acttttggaa atactggcgc aattcttgtt cttttacttc
     7021 tactgcatcg aagaccttgg catcacgctt aagtaatttg actaaccgtc ttttgctatc
     7081 cagctttcct tctgcaaaga ttatcaactt ggttgttgga gaaggattgt caaggtattc
     7141 ctcaaatgac ttaagctcat catctgtcaa aaagcgtttc ttagcagtcg tgatatccat
     7201 aaaataatct aatatcacga ttttctcatc cgcaaagaaa ggaaggctga ccaactccag
     7261 ttccacatcc ttgtaaacta cttctttcat atcaaagtag gcaaagttga ggtcagcaga
     7321 atcataccca atctgtttca acacttgact cttcatcact tcaaactgac cctgatctgt
     7381 ccctgtaaat aggctcaggc tcggtaaatt tgataaagtc aacttctgac tttcttcaat
     7441 ggctagcatc gtctctcctt tcttcagatt tttcgattta atttagtcaa tatagcgcaa
     7501 tttcccacgg aaatcttcta agctctcgta gcctttttcc accatgattg ctttcagttc
     7561 attggtaatg cggtcaaaag cactgacgcc ttctttgtga agggtcgttc ccacctgcac
     7621 catacttgct ccacagagga tgtgttcaaa ggcatctcga ccagtcagaa cgccacctgt
     7681 tccgataatt tggatttgag gatttaaacg ttgataaaag gcgtgaacat tggctagagc
     7741 agtcggtttg atgtattctc caccaattcc accaaaacca ttcttaggcc gaataacgac
     7801 agattcgtct tctatataga ggccgtttcc gatagagtta acgcagttga caaacttgag
     7861 cggatatttg ttgaaaatag ctgccgcttg atcaaagtga acaatatcaa aataaggtgg
     7921 caatttaatt ccaagaggtt tggtgaagta agcaaacact tctgccaaaa tccggtctgt
     7981 tgtctcaaaa tcataggcaa tctgaggttt acctggaaca tttggacagg aaagatttag
     8041 ctcagtcaga ccacgaaaat cactctcttg gacttttttc aaaatagtat gggtttcctc
     8101 tggagacatg ccgaccagag ataagaagaa agttcggttc gactcttttt cctgcaaatc
     8161 taaaagataa tccaaataat agtctaagcc attatttggc aagcccatag agttgatgga
     8221 accaagtgga acatcttggt agcgtggctc aggattcccc tgacggaagt ccaaggtcgc
     8281 tgtcttagta acaaaggttc ctgccgctga gtttttgacc tcttctaact cctctatcgt
     8341 catacaagcc acacctgctg cattcatcaa gcaattgtca aactcaaaac cagcaatttg
     8401 tgttttcgtt gataccatga ttctaatcct ccagatttct tttattgcta atattatacc
     8461 ataattttaa ttctaggttt actagattct gtggttctat ctatttattc ggaaaagaaa
     8521 gtctagtaca gaagtcatgc tatggaaaca gctagtgtat tagaagtttg aacaaggctt
     8581 gggtaacaga cttttgaatg aagtccaagc aacaaggaga aatctcaacc atgagtccat
     8641 tccatgactc tgttgctcta gaaaaccttg caatagtcaa gtgataacga ataaaacttt
     8701 ggattgaaat atccccaaga ttatctatta cgaggagaaa tcttaacctt tatcattttt
     8761 aaagaaatat agtaaaatga aacaagaaca ggagaaatcg atcaggacag tcaaattgat
     8821 tgctaacaat gttttagaag ttgaggtgta ctattctagt ttcaatctac tatacatgta
     8881 aatctctact ttcgcaaaat ggattttcag aacagtatcc cagtatagtt aaaaactata
     8941 aaaaagagag gacgttcccc tcttaaaatc ttattttact gcttctttca aagcatctac
     9001 cttatccaaa cgttcccatg gaagatcaat atctgtacgt cccatgtgac cgtaagccga
     9061 tgtttgacgg taaattggac gcttaaggtc cagcatttgg ataatccctg cagggcgaag
     9121 gtcaaagatt tgacgagccg ctttttcaag ttgactttca gctactgttc ctgtaccaaa
     9181 agtatcgata cgaacagaaa caggttgcgc aacaccgata gcataggcca actgcacttc
     9241 tgccttctta gcaaggtctg ctgcaacgat attcttggca atatagcgag ccgcataaga
     9301 ggctgaacga tccaccttag tcgcatcttt accagagaag gcaccaccac catgacgaga
     9361 gtagccacca taagtatcta caataatctt acgaccagtc aaacctgagt ccccttgagg
     9421 accaccgatt acaaaacgac ctgtcggatt gataaagaat tttgtcttat catcaagata
     9481 agaagatgga ataacttctt tgatgacctt gtcaatcaca tcttgatgga tttgttcatt
     9541 agtggcctct ggatcatgct gagtagaaat aacgactgta tctacacgta ccggacggtc
     9601 attttcatcg tactcaactg taacttgtga ttttgcatct ggacggagat agctaatttc
     9661 tccagactta cgaagttctg ccagacgacg aaccaattta tgactgagtg caattggcaa
     9721 tggcataagc tcttctgttt catctactgc aaatccaaac atgagccctt ggtcacctgc
     9781 tccaatcaag tccagtggat cttgatcagc atttccacga acctccaagg cttcgttaac
     9841 accttgagcg atgtcaggag attgttccac caaagatggg tgtactccca ccgtctcagc
     9901 agaaaatcca tattctgtat tggtataacc aatctctgca atggtatcac gaaccacacg
     9961 gttaatatcc acataggcat ttgtagaaat ttcaccaaaa acgtggacag aaccagtata
    10021 tacagctgtt tcagcagcaa cgtgcgcctc tggatccttt gctaaaatag catccaaaat
    10081 cgcatctgaa atttggtctg caatcttatc cggatgcccc tcagatacag attcagacgt
    10141 gaataattta cgctctgaca taaaaatgtc cccccttaaa aaatattgtt atagagttac
    10201 aaagtactga taggaaaact cttcgtaata aatacctacc atcttatcgg gactatataa
    10261 ctttatcatt ataccatttt taatcgcaat ttgcagtctt taatgtagtg tttcggttta
    10321 gatagttcgt tctcataaag caaaaagttg gagctcagta ctccaacaat ttctattaaa
    10381 atgtttgacg tttagcttta cgctctgcac gaccgcgagc gcgattttca gcacgcttgg
    10441 ttttacggcg cttttcatca accgcccatt gaattttctt cttataaccc ggtttgactt
    10501 ttttcttttt ctttttaacc aaaccaatca tttcgatatc aagtttatct tgttttttct
    10561 cacggttggc acgacgatca cggtcatagg tatcttgaaa ttccccgtct ttgaccatct
    10621 taggactaaa cttgattccc aatttctcca actcacggat atccgagtca tcacttggct
    10681 gataaagggt aatagctgta cctggtaggc catttcgtcc agtacgacca acacggtgaa
    10741 caaaaaaaga taagtcttgc ggaatggcat cattgatgac atggctgaca ccttcaatgt
    10801 caatcccacg cgctgccaaa tctgttgcga caatatactc aaaatccaga ttttgcacct
    10861 gattcatgat tcgcttgcgt tcacgagggg caatatcgcc atggattttt gcaaccttca
    10921 agccttgagc agtcagatat gaatgcaatt catcagcacg cgttttagtg ttaacaaaaa
    10981 tcattgccaa atacggctgc atcaactgag tcaactggta aatttgagca ttcttatcat
    11041 gtcccttggt cgaaatcaac caattatcaa tggtgtcaga aataaccgtt ttggtcttaa
    11101 ttttctccat aacaggattg gataagtatt ttttcaagaa tggttgcagt ttttgtggga
    11161 tagtcgctga gaagaccatg aattgcaagt cttttggaag actgccagca atcttatcaa
    11221 cagtttccaa gaatcccata tccaaggtca tatccgcttc atcaacaaca aatgtcttgg
    11281 ctttatgaat agctaaatca ccagatttaa ccaagtcgta gatacggcct ggtgttccaa
    11341 taacaatatg aggctgattg cttgccaatt tctcaatctg gcgagcctta tccgtaccac
    11401 ccacataatt aaccacacga acttcgacat ctgagtgagc tgaaatctga cgcgctactt
    11461 ggtaaatttg agtagccaac tcacgactcg gtgcagtaat cactgcttgt acactatcgc
    11521 tagcttcatc taattgctgg aaaatcggta acaagaaagt atgagtctta cctgaacctg
    11581 tttttgattc tcctactagg tcacgacctg ccaaaacaat aggaatcaac ttgtcttgca
    11641 cctctgttgg agttgtaaat tttaactcct ccaaggcttc tctaatatag tttttaaatt
    11701 gaaatttcgt aaatgacata acatcctcga ttctatctat cttatcaatt ataccatatt
    11761 ttattccatt acagtagtct cacttattta ggctatttcc agtagcttct ctagtaagaa
    11821 aaggctggaa ttttatagtt ccaacctctt ttcagttatt atttccagtt taacatagca
    11881 ttcaagccat agtgatcact cacttgtgga ctcttgttac catcaaatac gacatgtaaa
    11941 ttttccaccg ctaactcttt ggtagtaaag acataatcga ttcgaagggg ttcagtgttc
    12001 cctttccagc catcaatttc aggcggaaca gtatagctac cacttttctc ttgagcaact
    12061 tcaaatgcgt cttgtaagcc taatgaacta gctaaaatag cttggtaacc ttcctgacct
    12121 gctgggttgt taaaatctcc agctagtaaa agtggcttgt tcaatttttt caagacagcc
    12181 tcaaatcgtg cccattcttc ttggaaacct ttatcccacc aagagagatg aacactggca
    12241 actgctagct ccttgccatc gactacagtt tcagctaggg caacacggcg agtatgatag
    12301 tctgttggat catccacatc tgaaaccaaa atttctctgg cttcaatagg tgttttagac
    12361 aagatagcca caccttcgtg gtagcggtca tagccgatat ggttataggc ccaggtccag
    12421 tagtaatttt tcccttgctc agataacttt tcaaccaaga gtctaacata gtgatcttgg
    12481 tgaataggct cagctgctgg caaagcttga taaaggtcat taacctccac ctctgacgag
    12541 gtcatctcct gattaatttc ttggaaacaa atcaaatcat agtccttttc aagaatatct
    12601 tcaagcaaaa tctggaattt ttcctctgct tctttctcca tccaactatg agtattgagt
    12661 gttaaaaatt tcattctttt ctcccaaaat aagaggttgg aatacagctt ccaacctcaa
    12721 gtatattaca attctacttt agcaactgct gttttagctg caagagaacc tgttttttct
    12781 aatttaactg atttaattgc atcaccattt gtgaagacaa ctactgttga agtttcacgt
    12841 cctgctgcac ggatagcatc caagtcagct gtgacaagga gatctcctgc tgcaactttt
    12901 tgtccttcag caacatgaac tgtaaatggt ttaccttcaa gacttactgt gtccaaacca
    12961 atgtgaacca atacttcaag acctgcttcc gtcacaatac caaaagcatg ttttgttggg
    13021 aagatgcttg atacagtacc tgaaactgga gatacaatgt ttccatttgc gggttctact
    13081 gcaaatccat cacccatcat tttttgagca aatactggat cctttacttg ttccaaacca
    13141 acaacttgac cgtctgctac tgaataaact tcctcagtaa gatctttgaa gtgaacagtg
    13201 ttttgttgtg cttcagtcat ttggcttgga agagtttcag gaatgatttc acctgaatca
    13261 aggatatctt ggatatcaga tttcaatatg tcagcttttg gaccgtagat agcttgaacc
    13321 ccttgccctt tcatgacaag acccatagct ccttctgctt tccattgctc tgcatttcct
    13381 actttatctg catctttaac agttacacga agacgagtca tacatgcgtc aacatcaacg
    13441 atgtttacac gtccaccaag aaggttgata atgtttacag cttgagagcc tgctgcaact
    13501 ttcacttcgc tgctggtttc ttctgaacct tcagcagttt cgtagtttcc gttgcgccct
    13561 ggagttgcgt agttgaattt ttgaatcatg aagtttgcga taaagtacat gattacagca
    13621 aagagaacag ttacccaaac gaagttaacg atatccatac caataccagc actgattgca
    13681 ataggtgtac gagtcaagaa ctcgattgaa ccgaatgagt gcatacgtag gtttacgacg
    13741 tcagccatag cgaaggcagc accttgaaca agtgagtaaa caagatacat aggtgttgcg
    13801 atgaacatga acatgtattc gattggttca gtaacccctg tcaagaatgt tgcaagagct
    13861 gttgcaatca tcataccttt gtatttatgt ttcttgtcag catcaacatt acggtagata
    13921 gcaacaatca cacccatcaa gataccgaat gaaccgatca tttgtccaac tttgaaacga
    13981 gctggatgta ctgtatctaa caagtgttgg tattgactag catcagtacc tttaaggttt
    14041 acaaggtctg ttacccatgc aagccatagt gggtcttgac cgaatacttg agtaccttta
    14101 gctgcaccag ttaaaatgtc ataagtacca ccaagagctg tgtagttcat tgggatagtc
    14161 aacatgtggt gaagaccaaa tggcaagagc aaacgttcca aagtaccata caagaatggt
    14221 gcaagaattg gagcagtttc ttgtgagttg gcaatccaga taccgaagtt attgatacct
    14281 gtttgaacta ctggccagaa agcagcaagt agaattgcag cgattgctga acgaagaata
    14341 actacaaatg gtacgaaacg tttcccgttg aagaatgaaa gtgcatcagg aagtttacgg
    14401 aagttgtagt atttgttgta agcagttgcc cctacaaaac ctgagataat ccctacgaat
    14461 acccccatgt tcaaggctgg agcttcaaga acactgataa agtaatcagc aactttgatt
    14521 gaaccaccaa agaaagtagt taccatagca tctggatttt tcaacatatc gcctgataca
    14581 ccaaaaattg taccagtgat acggttaatc aagatgaagg caagaccagc ggcgaaagca
    14641 ccaccagcac gttctttagc ccagcttcct ccaatggcta gggcaaacaa aatgtgaagg
    14701 ttaccgataa ccccccaacc gatttgctca agaattccac ctgtgatgac aagtggtgta
    14761 aaggttgggt taatcatcac gatagactta ccgattgaaa tcatcaaacc agcagccggc
    14821 ataaccgcga taactaccat caaagcctta ccgaattttt gccaaaattc gaaagacaag
    14881 acatttttga atgtatcttt catcattcaa atctccttta tttttattta ggcaaacgtt
    14941 ttacgaaaac gtt
//
LOCUS       DAVGJL010000042        14747 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.042, whole genome shotgun sequence.
ACCESSION   DAVGJL010000042 DAVGJL010000000
VERSION     DAVGJL010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14747
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.042"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(78..539)
                     /locus_tag="VVE42_001029"
     CDS             complement(78..539)
                     /locus_tag="VVE42_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU3073237.1"
                     /translation="MGKERREQRNLEWKKRVVKKRKIPLEELKAFVEAHPDAFLREIA
                     ARFDCALPSVWAVLKQIKVILKKTTSFREQKPEKVSEFLDILDNLKDLPVLYIDETGI
                     NRYLYRPYAGAPRGEKVYDKISGRRFERTNEVEQKLNGSFLIRYIDSQIRE"
     gene            complement(506..649)
                     /locus_tag="VVE42_001030"
     CDS             complement(506..649)
                     /locus_tag="VVE42_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tnpB"
                     /protein_id="HEU3073238.1"
                     /translation="MAYSTDFKQGALDSIKEGHRHVEAAKVFDVGARTLFTWEKKDVN
                     KGT"
     gene            917..1297
                     /locus_tag="VVE42_001031"
     CDS             917..1297
                     /locus_tag="VVE42_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU3073239.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEETIAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTND
                     GQKTLASLQKTVEESLEAGLDFLI"
     gene            1395..1616
                     /locus_tag="VVE42_001032"
     CDS             1395..1616
                     /locus_tag="VVE42_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEU3073240.1"
                     /translation="MIEITYIDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLDYHGTYGDIFFYLMKQDLSQYN"
     gene            1633..1947
                     /gene="trxA"
                     /locus_tag="VVE42_001033"
     CDS             1633..1947
                     /gene="trxA"
                     /locus_tag="VVE42_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU3073241.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
     gene            2255..2788
                     /gene="queF"
                     /locus_tag="VVE42_001034"
     CDS             2255..2788
                     /gene="queF"
                     /locus_tag="VVE42_001034"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEU3073242.1"
                     /translation="MKRQSALVVFSGGQYSTTCLFWAMQHYETVEAVTFAYGQRHHLE
                     IQITREIAKEQGIRHHILDMSLLGQITAQPDFATIHISYIPDKLCVESKSLKLYLFSY
                     RNHGDFHENCINTIGKDLVNLLDPRYLEVWGKFTPRGGISIDPYYNYGKQGTKYEGLA
                     EQRLFQHDLYPEKIDNR"
     gene            2785..2874
                     /locus_tag="VVE42_001035"
                     /pseudo
     CDS             2785..2874
                     /locus_tag="VVE42_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(3178..3846)
                     /locus_tag="VVE42_001036"
     CDS             complement(3178..3846)
                     /locus_tag="VVE42_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEU3073243.1"
                     /translation="MKKFVAELIGTFMLVFVGTGAVVFGNGFDGLGHLGIAFAFGLAI
                     VVAAYSIGTVSGAHLNPAVSIAMFVNKRLSSSELVNYILGQVVGAFIASGAVFFLLAN
                     SGMSTASLGENALANGVTVFGGFLFEVIATFLFVLVIMTVTSESKGNGAIAGLVIGLS
                     LMAMILVGLKITGLSVNPARSLAPAVLVGGAALQQVWIFILAPIAGGVLAALVAKNFL
                     GTEE"
     gene            complement(3957..4748)
                     /locus_tag="VVE42_001037"
     CDS             complement(3957..4748)
                     /locus_tag="VVE42_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HEU3073244.1"
                     /translation="MYQDLLRKIAEEKPNYNQEEIQWLLDHLGDPSPEIRDDLVFTSF
                     ARGIQEELFTQEQFHFIAEGVSSDGGLDKEIDKVGLPTLERSFRALVYATLLSVDANQ
                     QSVFYQGLQSEIRNVLLNQGLHYLSKEKDTTGFSSQYGWVHSFAHGAYLLTEVVCHPD
                     FPKNRVHEVFDILGQLFKRMSIRFTDDEDWRLARVIYEPILQGKLEQEQVASWIKTVD
                     FPIEEREDFYKFSNFRSCLVEVYVQLDQRNSLQDDLTEAIQSFQY"
     gene            complement(5173..5937)
                     /locus_tag="VVE42_001038"
     CDS             complement(5173..5937)
                     /locus_tag="VVE42_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU3073245.1"
                     /translation="MFKLSFQSIGHVVVRNYMSFRNLFKISIVPNLIDPLFYLLAMGF
                     GVGAYLTHVNGMLYRDFVITGLIAATAMSAATAETTVNAFIQYKIEKTYDAMLMTPIN
                     TSDIVVGQAIWAGIRAVIFGGIFWLISMLITAHFHVLMLLIPLILFIVGYLFGVLGLI
                     FTYLAPSREFLNYYNVLVIRPLYMFSDTFFPITSLPPILGDLTWFSPLYHATRMIRII
                     WGGEVTGLYLHLIWLIALALLITVLPMYMLHRRYYR"
     gene            complement(5930..6700)
                     /locus_tag="VVE42_001039"
     CDS             complement(5930..6700)
                     /locus_tag="VVE42_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000966490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3073246.1"
                     /translation="MLYLWGKERVKKLSVKNLKKQYGSYEAVKGISFELGEGECLAIL
                     GINGAGKTSTLKMIYSLTSISSGEIDILGQNIETNRRKSKSCLGIVGQEDMLDMTLNV
                     YDNLFAHGLCYGISSVELRKRIDSLLDFVQLSKHAQKMVNQLSGGMRRRLVLARALIN
                     RPDIIILDEPTVGLDIQSRNIIWDKLLELKELGVSIIITSHYMNEIEYLTDRVLMLHQ
                     GTIKEEGTVEGLLIKYDADNLETLFWGLTGTEKEDLHV"
     gene            6947..7624
                     /locus_tag="VVE42_001040"
     CDS             6947..7624
                     /locus_tag="VVE42_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000433071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEU3073247.1"
                     /translation="MEPDIFIITKLLSDPSRMHILDILMDGKAHTVNEIASFTKIKQH
                     TVSYHLKLLTEAQVTTLQTYGRFHYYSLKSAAIAEMLEFLSFYSPQRDVKSYKQHIHK
                     KELKVARTCYDHIAGELGISITNYLLQKNLLSESEKDFQLTEAGKSYFQRKLHIDTDE
                     LKKKKRKFCPKCLDWSERKNHVGGALGNALLEFFSEKQLVIPAQTPRSLTITEKGKEF
                     LEKEWEI"
     gene            complement(7684..9480)
                     /gene="pepF"
                     /locus_tag="VVE42_001041"
     CDS             complement(7684..9480)
                     /gene="pepF"
                     /locus_tag="VVE42_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEU3073248.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFEDFEKAFAELEQIYIQMSHIGNYGFMPQTTDYSNDEFANIAQAGMEFETD
                     ASVALTFFDDALVAANEEVLDRLGKLPHLTAAIRQAKIKKAHYLGADVEKALTNLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFVTYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLADMKGYDSVFDYLLAEQEVDRVMFDRQIDLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEECWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKNSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIIAYSAELGE"
     gene            complement(9491..10234)
                     /locus_tag="VVE42_001042"
     CDS             complement(9491..10234)
                     /locus_tag="VVE42_001042"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEU3073249.1"
                     /translation="MQQYFVKGSAISPVTIEDKETSKHMFQVMRLKEEDEVTLVFDDG
                     IKRLARVLDMENRQFELVQELNDNVELPVQVTIASGFPKGDKLEFIAQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLAQLDQ
                     FDSIIVAYEESAKEGEAAALLQAVTGLETGAKLLFIFGPEGGLSPAEIESFEAKGAVL
                     VGLGPRILRAETAPLYALSALSVLVELEK"
     gene            complement(10236..11189)
                     /locus_tag="VVE42_001043"
     CDS             complement(10236..11189)
                     /locus_tag="VVE42_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000970787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEU3073250.1"
                     /translation="MMETWQELKVTVKREGEELVSNLLIELGAQGVAIEDSLDYVGNV
                     DRFGEIFPEVEQQEEIVVTAYYPDTVDVTVVEADLQARLAELTDFMDLGELKIGTTVL
                     AEEDWADNWKKYYEPARITHDLTIVPSWTDYEATAGEKIIKLDPGMAFGTGTHPTTKM
                     SLFALEQVLRGGETVLDVGTGSGVLSIASSLLGAKEIFAYDLDDVAVRVAQENIELNP
                     GMENIHVAAGDLLKGVEIEADVIVANILADILIHLIDDAYRLVNDEGYLIMSGIIKDK
                     WDMVRQSAESAGFFLETHMVQGEWNTCVFKKTKDISGVIGG"
     gene            complement(11324..11752)
                     /locus_tag="VVE42_001044"
     CDS             complement(11324..11752)
                     /locus_tag="VVE42_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU3073251.1"
                     /translation="MELEISDFPGCKIALFCEDKLLTILRDDKASIPWANMWELPGGG
                     REGDESPFECAAREVYEELGIHLTEDCLLWSKVYPSMLFADKQSVFLVGQLTQNQFDS
                     TVFGDEGQGYQLMNVEEFLSSSQVVPQLQERLKDYLKVSD"
     gene            complement(11733..12821)
                     /locus_tag="VVE42_001045"
     CDS             complement(11733..12821)
                     /locus_tag="VVE42_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEU3073252.1"
                     /translation="MKKIGTYLVYVLAVVFIMLAFACGTIAFAELGYSAVLAFTFGYA
                     FALLSMYLIFILHELGHAFCGYLTGYRLVAFGLGHFILTKKSGRFHLSRTAILKNVGA
                     QYIGLKEDESDQRIILMLSGGLMVHLGLILLAILFGFLTRSWYFAGTWIFLNLSFFLN
                     NILPVDITDGAKIWELLQHPENTKYAYLVLRHSAQTLLAPQEYDLKDFVQAVPEDARG
                     SFADGVLGMQGEVSILEGKEEVAKQQFQALLERTTTPMMQTVAQLSLLHIALLEGDFE
                     QAEQYASIRQVKSFLSLKLSNLQVIQAWYQFKVKKDVDRTRKALKIARQKMDSSRMLR
                     DEKRYYENLLAELEKEMLEGTEDGTRNF"
     gene            complement(12840..13310)
                     /locus_tag="VVE42_001046"
     CDS             complement(12840..13310)
                     /locus_tag="VVE42_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEU3073253.1"
                     /translation="MATYGFLDVLEEELDKNFPFDFEISWDKRNHAVEVSFLLEAQNA
                     AGVEMVDEDGEVLSDDILFEEAVLFYNPAKSTVNEEDYLTVIPYLPKKGFSREFLAYF
                     ALFLKDTAEVGLDVLMDFLEDPEAEEFVMEWNQEVFEEGKVGLEEGEFYPYPRY"
     gene            complement(13601..14053)
                     /locus_tag="VVE42_001047"
     CDS             complement(13601..14053)
                     /locus_tag="VVE42_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEU3073254.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            complement(14090..14269)
                     /locus_tag="VVE42_001048"
     CDS             complement(14090..14269)
                     /locus_tag="VVE42_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEU3073255.1"
                     /translation="MPMTQKEMVKLLTAHGWIKTRGGKGSHIKMEKQGERPITIPHGE
                     LNKYTERGIGKQAGL"
     gene            complement(14406..14585)
                     /locus_tag="VVE42_001049"
     CDS             complement(14406..14585)
                     /locus_tag="VVE42_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073256.1"
                     /translation="MNLIFEEYQFVKIGDKKYRVRSVPMIHSTPPQSVLDRDTFTIDY
                     TDDELLDKEAVFTTH"
BASE COUNT         4569 a         3346 c         2629 g         4203 t
ORIGIN      
        1 ttttataaag acacgtaact gagtataaat cagctaattt ttggtttata ttcctgtttt
       61 tatgcataat tattctgtca ttctctaatt tgtgaatcaa tatatctgat tagaaaacta
      121 ccattgagtt tttgctccac ctcattagtc cgctcaaaac gccgtccgct aatcttgtca
      181 tagactttct cccctctagg agcccctgca taaggacgat agaggtagcg gttgattccc
      241 gtttcgtcaa tatataggac tggtaaatct tttaggttat ccaaaatatc aagaaactca
      301 gaaactttct caggcttttg ttccctaaaa ctggtcgtct tttttaaaat gaccttaatc
      361 tgctttaaaa ctgcccatac ggagggcaaa gcacaatcaa aacgggctgc aatttcccgt
      421 aaaaaagcgt ctggatgagc ctctacaaag gctttcaatt cttctaaagg gatcttacgc
      481 tttttgacga ctcgcttttt ccactctaag ttcctttgtt cacgtctttc ttttcccacg
      541 tgaagagagt tctggcgcca acatcaaaaa ctttggccgc ctcgacatgt ctgtgcccct
      601 ctttgatgga atctaatgct ccttgtttaa aatcagtact atatgccata gctttattag
      661 accatgctta ttttaaacta agcaactata gatagagaaa tgaaataaaa tctgaattcg
      721 aatgtgataa ggagttaaaa attgaaacaa taggattccc ccattattca cacaaaataa
      781 aaccactaaa aaacagagct tgccagcatt cttgaaaagt agtataatta ttgcatgcgc
      841 aatcatcttg tgacgcagag actgcccgtt aatggacttt cttctatttt atgactctaa
      901 aaagaaagga gacactatga cctatttgga aaaatggttt gacttcaatc ggcgtcagaa
      961 agaaattgaa agtctcttgg aagagaccat tgcccaacag agcgagcaaa gtctgacctt
     1021 gaaagagttc tacctgctct actatctgga cttggctgaa gaaaaatctc tacgccagat
     1081 tgacctgcca gataagcttc atctgagccc gagcgcagtt tctaggatgg tggctcgctt
     1141 ggaagctaaa aattgcggtc tactcagtcg catgtgttgc catcaagata gacgctctag
     1201 ttttatctgc ctgacaaatg atgggcaaaa gacactggcc tcgctacaaa agactgtcga
     1261 agaaagcttg gaagcaggtt tggatttctt gatttaagat gatttaaaaa aagttgcgtg
     1321 cgcaatcatt tttcttgaca ttctctttta taaggagtaa aataaagtta gcattaaaca
     1381 aaggagtttt aaatatgatt gaaattacct atatagatgc cagcaagaac gaaagaactg
     1441 tgactttcga gtcttatgaa gactttgaac gttcgcaaca agcttgcctt atcggcgtcg
     1501 cagactacta ccctgtccaa aaattaactt acaagggtca taatttggac taccatggga
     1561 cttatggaga tatcttcttc tatctcatga aacaagattt aagccaatat aactaaaaaa
     1621 aggagaaata caatggcaaa agcaattaca gatgcaacat tcgaacaaga aacaaaagac
     1681 ggtttggtct tagtagactt ctgggcaact tggtgtggtc catgtcgtat gcaaggtcca
     1741 atcttggaca aattgtctga agaactttca gaagatgtct tgaaaatcgt taaaatggac
     1801 gttgatgaaa atccaaacac agctcgtgct tttggaatca tgtctattcc aactcttctc
     1861 ttcaaaaaag acggccaagt tgtcaaacag gttgcaggtg ttcacacagc agaacaaatc
     1921 aaggccatca ttgctgaatt gagctaatca aacgagagac caagtacttt ctttggtctc
     1981 ttttttcttg ccctttgcca tttttcaaaa aatatgctag actatagcag attgaaacta
     2041 gaatagtaca cctctacttc taaaacattg ttagaaatcg atttgactgt cctgatcgat
     2101 ttgtcctgtt cttatttcat tttactatat aagtagaata ttacgatgtt tgggatatgc
     2161 catcgctaaa aaaaaacctc gatttggtat tttttagctc cctaatgaga gctttttgcg
     2221 tgctctgaac atttcccatt ttggaaggag tactatgaaa cgtcaatcag ccttggtcgt
     2281 ctttagtggt ggtcaatatt caacaacctg ccttttctgg gccatgcaac actatgaaac
     2341 agtcgaagct gtcacctttg cctacggcca acgtcatcac ctcgaaattc aaattactag
     2401 agaaatcgct aaggaacagg gcattcgtca ccatatcctc gatatgtctc tgctgggaca
     2461 aatcactgct cagccagact ttgcgacgat tcatatttcc tacattcctg acaagctctg
     2521 tgtcgagtca aaatccctca aactatatct atttagctac cgaaaccacg gagatttcca
     2581 cgaaaactgt atcaacacca tcgggaaaga cttggtcaac ttgctagacc ctcgctattt
     2641 agaagtctgg ggaaaattca ctccgcgcgg tggcatttca atcgacccct actataacta
     2701 cggtaagcaa ggaactaagt atgagggctt ggcagaacaa cgcctcttcc aacacgacct
     2761 ttatccagag aaaattgaca accgctaaag tcatactcaa tgaaaatcaa agagcaaact
     2821 aggaagttag ccgcaggttg ctcaaagcag tgttttgagg ttgtagatga aactgacgaa
     2881 gtcagctcaa aacactgttt tgaggttgca gatagaactg acgaagtcaa cttaaaacac
     2941 tgttttgagg ttgcagatag aactgacgaa gtcagcttaa aacactgttt tgaggttgca
     3001 gatagaactg acgaagtcag cttaaaacac tgttttgagg ttgcaaatag aactgacgaa
     3061 gtcagtaacc atacctacgg caaggtgaag ctgacgtggt ttgaagagat tttcgaagag
     3121 tatcatacga aaaagccctg ttcctcaaga tgaggagcaa ggctttttga gtttcaatta
     3181 ttcttctgtt ccaaggaaat tttttgcaac aagggctgca agaactccac cagcgattgg
     3241 tgcaaggatg aaaatccaaa cttgttgaag ggctgcgccg cctaccaaga cagctggtgc
     3301 caagctacga gctgggttta ctgaaagtcc agtaatcttc aatccgacaa gaatcatcgc
     3361 catcaatgac aaaccgatta ccaaaccagc aatcgcgcca ttgcccttgc tttctgaagt
     3421 cacagtcatg ataaccaata caaacaagaa agttgcgatg acttcaaaca agaaaccacc
     3481 aaagacagtg acaccgtttg ccaaggcatt ttcaccaaga ctagcagttg acatacctga
     3541 gttagccaag aggaagaaaa cagcgccaga agcgatgaaa gctccaacaa cctgaccaag
     3601 gatgtagttt acaagttctg aagatgacaa acgtttgttt acaaacatag caatcgaaac
     3661 agccgggttc aagtgagcac ctgaaacagt tccgattgag taggctgcca ccacgattgc
     3721 caaaccaaag gcaaaggcga ttccaaggtg accaaggcca tcaaaaccat ttccaaaaac
     3781 aacagctcct gtcccaacga acacaagcat gaacgtaccg attaactcag caacaaattt
     3841 tttcattttc tttctccttt tttcaaaaac tagatactag tctatcaaaa gtaggaaagg
     3901 gtttcaagaa aattgattgg aaattttttg aaaatcatag aactattagc taatccctag
     3961 tattgaaaag actggatagc ttctgtcagg tcatcttgta aactatttct ctggtcaagt
     4021 tggacataga cttccaccag acaggatcta aagttggaaa atttgtaaaa atcctccctt
     4081 tcttctatcg gaaaatcaac agtttttatc caagaagcta cttgttcttg ctccaacttc
     4141 ccttgtaaaa taggttcata gatcactctt gctaaacgcc aatcctcatc atctgtaaag
     4201 cgaatcgaca ttcttttaaa tagttggcca agtatgtcaa atacttcatg aactctgttt
     4261 ttaggaaagt ctggatgaca aaccacctct gtcagtaaat aggctccatg tgcaaaagaa
     4321 tgaacccaac catactgact tgagaaacct gttgtatcct tttcttttga aagatagtgc
     4381 aagccttgat ttaaaaggac attacgaatt tctgattgta atccctgata aaaaaccgat
     4441 tgctggttag catctacaga caagagagtt gcataaacaa gtgccctaaa agaacgttca
     4501 agtgttggca gacctacctt atcaatctct ttatctagtc ctccatcaga tgaaacccct
     4561 tcagcaatga aatgaaattg ctcctgtgta aatagctctt cctgaatccc tctagcaaag
     4621 cttgtaaaaa caagatcatc acgaatttct ggagaaggat ctcccaaatg atcaagcaac
     4681 cactggattt cctcctggtt ataatttggt ttttcttctg ctatttttct tagtaaatct
     4741 tgatacatgg tcaatacctc tacatttcta gcaactgttc aaaaaggcag tcttaaatga
     4801 ccttaccaat ttgtaaaatt aagttaaaaa gaaaaagaca tttatattca gagaaacagc
     4861 atatttctga aaagatatat aaatgattta ttccatatta caacaaataa acttgtaatg
     4921 atagagcctt atcacaaaaa tcacattcct gtctcaaaaa ttgcgaaaaa ctagttataa
     4981 taaagtgaaa taaaaactat acaaattgat tagggaattt aaaacaattt ctaacaatat
     5041 tttaaaaact gaaccgtact atttcaactt ccataaatta caatattgat aggcacagtt
     5101 cccatacctg tccaaattcg aataacgttt agtataaatt agaatatgac tacaacatca
     5161 caaataatta gactatctat aatatctacg atgtaacata tacataggaa gcaccgttat
     5221 caagagcgcc aaggctatca accagataag atgaagatac aaacctgtca cctcacctcc
     5281 ccatataata cgaatcatgc gagtagcatg atacaaggga gaaaaccaag tcaaatcacc
     5341 aagtattggg ggcaaggaag taatgggaaa gaaggtatct gaaaacatat aaagaggacg
     5401 gattaccaaa acattgtagt aattcagaaa ttccctagac ggcgccaagt aggtaaagat
     5461 tagtcctaaa acaccaaaca aataacccac tataaataaa atcagaggaa tcaataacat
     5521 caagacatga aaatgcgctg tgataagcat ggaaatcagc caaaaaatcc caccgaaaat
     5581 cactgcacga atacctgccc agatagcctg acccaccaca atatcactcg tattgattgg
     5641 cgtcatcaac atagcatcat aagtcttttc aatcttatac tggataaagg cattgaccgt
     5701 tgtttctgcg gtggccgcag acatggcagt cgcagcaatt aaacctgtaa tgacaaaatc
     5761 acggtagagc ataccgttaa catgcgtcaa ataggcacca actccaaatc ccattgctaa
     5821 aagataaaag aggggatcga taaggttggg gacaattgaa attttaaata aatttctaaa
     5881 actcatgtag ttgcgtacca caacgtgtcc gatggactga aatgataact taaacatgca
     5941 agtcctcctt ctctgtccca gtcaaccccc aaaaaagcgt ctcaaggttg tcagcatcat
     6001 acttgatgag caagccttct acagttcctt cttctttgat agtcccttgg tggagcatca
     6061 gcaccctatc tgtcaaatat tcaatctcat tcatatagtg ggaagtaata atgatggaaa
     6121 cacctaattc cttcaactcc aaaagtttat cccaaataat attgcgggat tgaatgtcta
     6181 aaccaactgt cggttcatct aaaatgataa tatcaggccg attgatgaga gctcttgcca
     6241 agacaagacg tcgtcgcatc ccacccgaca gctgattcac catcttttgc gcatgtttgc
     6301 tcagttgaac aaaatccaag agagaatcga ttctcttcct aagttcgaca gatgagatac
     6361 cataacatag accgtgagca aaaagattat cataaacatt caaggtcatg tccagcatat
     6421 cctcctgccc tacaataccc agacaagatt tactcttacg acgattggtt tcaatatttt
     6481 gccccaagat atcaatctct cctgaggaaa ttgatgtcag tgagtaaatc atctttaaag
     6541 tggatgtttt gccagcaccg ttgattccca agatagccaa acactctcct tctcccaatt
     6601 caaagctaat tcccttgaca gcttcataag aaccatattg cttttttaga tttttaacac
     6661 ttaacttctt cactctttct ttcccccaca aatagagcat gttggatact ttgttaattc
     6721 atctcacttt tgaaaaaatg ttgagaatgt aatcttaaga ttaacgtcct tcaactgtgg
     6781 atactgctgc aattactttt gtaattttac ctccatttaa actcagacaa taaaatttaa
     6841 aatattctct agtaaaacat ttttaaaatc tatttttctt gtgctttaat tatagtataa
     6901 taaaagatag aaaatcatcg atatatgaaa tttggagaaa atttttatgg aaccagatat
     6961 tttcatcatc acaaaactct tatcagaccc ttctcggatg cacattttag atattctaat
     7021 ggatggaaaa gctcacacgg ttaacgaaat tgccagcttt accaaaatca agcaacatac
     7081 tgtcagctat cacctcaaat tacttaccga agcgcaggta accactctac aaacctatgg
     7141 acgttttcat tattattctt taaaaagtgc tgccattgca gaaatgctgg aatttctcag
     7201 cttctactct cctcagcgtg atgtcaaaag ttataaacaa cacattcaca aaaaggaatt
     7261 aaaagtcgct cgcacctgct atgaccatat tgctggtgag cttggcattt ccattaccaa
     7321 ttatctcttg caaaaaaatc tcctcagcga atcagaaaaa gacttccaac tcactgaagc
     7381 tgggaagtct tatttccaaa gaaaattaca cattgataca gatgaattga aaaagaaaaa
     7441 acggaaattt tgccctaaat gtttggattg gagcgaacgt aaaaatcatg tcggcggagc
     7501 tctcggcaat gctcttttag aatttttcag tgaaaaacaa ttggtcatcc ctgcacaaac
     7561 gccacgttca cttaccatta cagagaaggg caaagaattc ctcgaaaagg aatgggagat
     7621 ttaaacatgg acttttaagc aaataactgt ccatacccat tctataacaa cctctctatc
     7681 cccttactcc cccaactcag cactgtaggc gataatctgg tcaactgtgt cagacaagaa
     7741 ttggatggta tcacggagtg gtttgtctgt tgaaatatcc gcaccgataa tcatggctga
     7801 ctcaagtggt gtcttgctac cacctgattt gaggagattg agccagtctt cagctccagt
     7861 ttctgagttt ttaagatgaa ggtaaccagc tgtcgagatg acaagaccag cagagtaagt
     7921 gtaactatac aagcccatat agtagtgagc ttggcgcatc caagtcagag ctgcatcatc
     7981 atcaatttca atagcatctc cccagaaatc cgtcaaaact tccttcataa tgctgttgag
     8041 cttgcttgct ccaaaggtct ccccttcttc aatcaatgta tacaccttac gctggaaggc
     8101 ggcttccaag aggtgggtga tgaaattatg gaagtaggtg tctgtcaagc gatgagccag
     8161 agcgaagcgt ttttgacgtg ggtcattaga ctggttctct aagtaatcac tgagtagcaa
     8221 ttcattgaag gttgacggtg cttcaacata ataggtcgac atgtgggcgt tgaaatagct
     8281 ttgatgattg tctgaaaaga tgaattgacc agaatgcccg atttcatgaa tcaaggtata
     8341 gacatcgctc aaacggcctg tccagctcat gagtacataa gggtgtacgc gatatgggtc
     8401 cgccgcataa ccaccggaat ccttgccact gttagcagca aagtccaccc agcactcttc
     8461 ttggtaacga gcaacttcct gacaatattc ttgccccaaa ggttctaccg acttcatgac
     8521 caaatcatag gcatcgtcaa tagtcacttc aggattgagg gcgctgtcca agtccaattt
     8581 ccagtctgca aaggtcatct tttcaagacc atttaccttg gcaacatgct tgaggtatct
     8641 ctgagcgact ggtgcaaagt ccttcatgat gaggtcaatc tggcggtcaa acatgacacg
     8701 gtccacttct tgttcagcta gaagataatc aaagacagag tcgtatccct tcatatcagc
     8761 caagagtttt tcagacttga cctgagccag ataggctgct gcagccgtat tttggtgctt
     8821 acgaagtccc tctgagaagg aacggaagga tttctcacga acctcagcat cctcgtgatt
     8881 ttggtagaaa ttctcatagg tgacaaagct gtttttatag gtcttgccat gggcttcaaa
     8941 gtcagccatt tcaaaatccc cagctcgcat cttagtataa atgtcctgcg gactgtagaa
     9001 aacttcaccg agattggtca aggccttctc cacatcagcc cctagatagt gggctttttt
     9061 gattttggcc tgacgaatag cagctgttaa atgtggcaat ttacccaaac ggtccaagac
     9121 ttcctcattt gctgccacca aggcatcgtc aaagaaggtc aaggctacgc tggcatctgt
     9181 ttcaaattcc atcccagctt gggcaatatt ggcaaattcg tcattgctat agtccgtcgt
     9241 ctgaggcata aaaccatagt tgccaatatg gctcatctga atgtagattt gctccaattc
     9301 cgcaaaggcc ttctcgaaat cctcaaaagt atgaagatta cccttgtagt cacggctaaa
     9361 ttggttgatg tcttcacgag ctttctcgat tgcacgcaag aaatcctcac ggtcttggta
     9421 tagggctgtt aagtcccaga gttccttctc tggaaattct gaacggtgtt tttgttccat
     9481 tttcttcctc ttatttctct aattctacta aaacactaag ggctgataag gcgtaaagcg
     9541 gtgctgtttc tgctcgcaaa atacgaggac ctaggcctac caaaacggct cccttagctt
     9601 caaaactttc gatttctgca ggtgacagac cgccttctgg accaaagata aagagcagtt
     9661 tggctcctgt ttcaagacca gtgactgctt gcagaagcgc agcggcttct ccttctttag
     9721 ctgattcttc ataggctact atgatagagt caaactggtc cagctgagct agaaaatctg
     9781 cttttttctc gaaaagttta atacttggta caatattacg cttgctttgc tcggctgctc
     9841 caagggcaat tttttctagt ttttcaactt ttttacccaa tttcttgcca tcccacttgg
     9901 caactgacca gtctgcagga aaggcccaga tttggctagc acccagttcg gttacttttt
     9961 gagcgatgaa ctccagcttg tctcccttgg gaaatccaga tgcgatggtc acttggactg
    10021 gtagttccac attgtcattt aattcttgga ccaactcaaa ctgacgattt tccatatcca
    10081 gcacgcgcgc caagcgcttg atgccatcat caaagactaa ggtaacctca tcctcttctt
    10141 tcaagcgcat aacctgaaac atatgcttac tggtttcctt gtcctcgata gtgacaggag
    10201 agatagcact gccttttaca aaatactgct gcatgctagc ctccaatcac accagagata
    10261 tccttggttt tcttaaagac acaggtattc cattcccctt gaaccatgtg agtttcgagg
    10321 aaaaatccag ctgattcagc cgactggcgc accatgtccc acttgtcctt gataatgcca
    10381 ctcatgatca ggtaaccttc gtcattgacc aaacgatagg catcgtctat tagatgaatg
    10441 aggatatccg ccaagatatt agctacaatc acatctgcct caatttccac acccttaagc
    10501 aaatctccag ccgctacatg gatattttcc atgccagggt tgagctcaat attttcctga
    10561 gccacacgaa ccgccacatc atccaggtca taggcgaaaa tttctttagc ccccagaagc
    10621 gagctggcaa tagagagaac ccctgaacca gtccccacat ctagcaccgt ttcgccacca
    10681 cgaagaacct gttccaaggc aaaaaggctc atcttggtag ttgggtgggt tccagtacca
    10741 aaagccatgc caggatccag cttgataatt ttttctcccg cagtcgcctc atagtctgtc
    10801 caagagggaa cgatggtcag gtcatgggtg atgcgtgccg gttcataata cttcttccag
    10861 ttgtcagccc agtcttcctc agccaagaca gtcgtaccta tttttaactc tcccaaatcc
    10921 ataaaatccg tcaattctgc taagcgagcc tgcaagtccg cctcaaccac tgtcacatcc
    10981 accgtgtcag ggtagtaggc tgtcactacg atttcttctt gctgctccac ctctgggaaa
    11041 atctcgccaa agcggtcaac atttcccaca tagtccagac tgtcttcaat cgcaacacct
    11101 tgcgctccca gctcaatcaa gagattggaa accaactcct ctccctcacg cttcactgta
    11161 acttttaact cttgccatgt ttccatcatt aagataccaa gcccgtaaaa cacaaaacca
    11221 aaataggaaa ttctctgaag acgcttgtgt ctaagagaag tttatctttt tggcacagtg
    11281 tttagggcgg gttcagttta gaaatgtaac tgaaccatcc tttctaatca cttactttta
    11341 aataatcttt taatctctct tgcaactgag ggacaacttg actggaacta agaaattcct
    11401 caacattcat cagctgatag ccctgtcctt catctccaaa tacggtactg tcaaactggt
    11461 tctgtgtcaa ctgaccaact agaaatacag attgtttatc cgcaaaaagc atacttggat
    11521 aaaccttact ccaaagcaga caatcttcag tcagatgaat gcccagttct tcataaactt
    11581 cacgcgccgc gcactcaaaa ggactttcat caccttcacg tcccccacct ggcagttccc
    11641 acatattggc ccagggaatg cttgccttat catcacgtaa gatagtcaag agcttatcct
    11701 cacaaaacaa agcaatctta cagcctggga aatcagaaat ttctagttcc atcttcagtt
    11761 ccttctaaca tttccttttc cagctctgct aacaagtttt cataatagcg tttctcgtcc
    11821 ctcaacattc gactactatc cattttctgt ctagcaatct tgagagcctt acgagttcga
    11881 tctacatctt tctttacctt aaattgatac caagcttgta tcacttgaag attggacagt
    11941 ttgagagaca gaaacgattt gacctgtcga atactagcat attgctccgc ttgctcaaaa
    12001 tctccttcca acaaggcgat atgaagcagg gatagttggg caactgtctg catcatcgga
    12061 gtagttgtcc tctcaagtaa tgcttgaaac tgctgtttag ctacttcttc cttcccttcc
    12121 aaaatggaaa cttcaccttg catacctaat acaccatccg caaaactccc tcgtgcatcc
    12181 tcaggaactg cttgaacaaa gtctttcaaa tcatattctt gaggagccag caaggtctgg
    12241 gcagaatgtc tcaataccag gtaggcgtat ttggtatttt cagggtgttg tagtaattcc
    12301 caaatttttg ctccatcggt gatgtcgact ggtaaaatgt tatttaggaa gaaagataaa
    12361 ttaagaaaaa tccaagtccc tgcaaaatac cagcttcttg tcaaaaatcc aaacaatatc
    12421 gccaataata tcaagccgag atgaaccatc aagcctcctg aaagcatcag gatgattctt
    12481 tgatcgcttt catcctcttt taaaccaatg tattgagcac caacattttt cagaatggct
    12541 gttctactaa gatgaaacct gcctgacttt ttggtcaaaa taaaatgtcc taatccaaaa
    12601 gccaccagcc gatagcctgt caagtagcca caaaaagcat gacccagctc atgaagaata
    12661 aagattaaat acatgcttag aagagcaaag gcataaccaa aagtaaaggc taaaactgcg
    12721 gaatatccca actctgcaaa tgcgattgtt ccacaagcaa aagctagcat aataaagaca
    12781 acagctagca cataaaccaa ataagtccca attttcttca taacacctcc aaccaactcc
    12841 tagtatcttg gataagggta aaattctccc tcttccaagc caacttttcc ttcttcaaag
    12901 acttcttggt tccattccat gacaaattcc tctgcttctg ggtcttccaa aaagtccatg
    12961 aggacatcta gcccaacctc agcagtatct ttaaggaaaa gcgcaaaata agctaaaaat
    13021 tcacgggaaa atcctttttt aggtagataa gggataaccg ttaaatagtc ttcctcattg
    13081 actgttgact tggcaggatt gtagaaaagg accgcttcct caaaaagaat gtcatctgat
    13141 aaaacctctc cgtcttcatc caccatctcc acacctgcag cattttgcgc ttccaataga
    13201 aaactcactt ctaccgcatg gttgcgtttg tcccagctaa tctcaaagtc aaagggaaag
    13261 ttcttgtcca actcttcctc taaaacatct aaaaatccgt atgttgccat tttgtcctct
    13321 ttctatgcga ctctttaatc gccccgattg ctcggaaata tgctaaaata gatactacca
    13381 tcttaccaca aaattatttt atgtcctaat tataccatat tacctcattt aaacccttgg
    13441 tatcagtgat tttcttaaaa gtctgatttc ttcatttctc ataaaaatca atataaaaag
    13501 cccgcgaaag ggctaataaa tctataaaat caataggcga gtaactagca caagtggacg
    13561 tgctttttta ttgactatta ccacgatacc acgcttaatc ttaggcttga actttcttat
    13621 ctgcaatagc gtctgtcaaa gtctgagaaa agttaagtcc catttctcgt cccaacttat
    13681 ctgcccattt tggtatggtc aaagtctttt taatgggttc ctgacttcct aggtattctg
    13741 atacatcaac agataccata gaaataaaag atttatcaag gtcataggtt gacacgaaat
    13801 cttcatcatc tttaaaagga tcattatcaa ttaaagacaa gctattgata tctgatggct
    13861 gaggtaactc tccatcactc tctatcaaat ctgcaacagt tatccctagc cactccgacc
    13921 ccatagccaa agcctcagaa atcccctctc cttgtgtagc tgagtattca aaatctggga
    13981 aatggacaaa ataagtcgct tctgttccgt ctgtgtcgtc ataataaaat aaagctggat
    14041 acgtaactaa catttcacta cctccatatc aaaaagcagg gactgaattt tacaacccag
    14101 cttgctttcc tatccctctt tcagtgtact tattcagctc accatgaggg attgtgatag
    14161 gtctttcccc ttgcttctcc attttaatat gggagccttt accgcctcta gtctttatcc
    14221 aaccatgggc cgtaaggagt ttaaccatct ctttttgtgt cataggcata gcgcttttac
    14281 ctcctgacaa caccattata acacgtgtta cacgtattgt aaaggagtga tacttattat
    14341 tctattatac ataaaagccc ctagatgtgg ttctaaggga aaccaattta ttcagaccta
    14401 tttttctaat gagtagtaaa aactgcttct ttatcgagca attcatcatc tgtatagtca
    14461 attgtaaaag tatctcgatc taagacagat tgaggcggag ttgaatgaat cataggaaca
    14521 ctgcgtactc tatatttttt atctccaatt tttacaaact gatactcttc gaaaatcaaa
    14581 ttcaaaccac gtcaacgtcg ccttaccgta ctcaagtaca gcctgcggct agtttcctag
    14641 tttgctcttt gattttcatt gagtatgatt aactctcaag tcttcgaaat caggattttc
    14701 aacagttatt acaaggaggc gatttactac ttcaaaaaca tcaatta
//
LOCUS       DAVGJL010000043        14732 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.043, whole genome shotgun sequence.
ACCESSION   DAVGJL010000043 DAVGJL010000000
VERSION     DAVGJL010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14732
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.043"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(45..1052)
                     /locus_tag="VVE42_001050"
                     /pseudo
     CDS             complement(45..1052)
                     /locus_tag="VVE42_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(1497..1895)
                     /locus_tag="VVE42_001051"
     CDS             complement(1497..1895)
                     /locus_tag="VVE42_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU3073257.1"
                     /translation="MENKESHKSQEIGNLIRVYNRSKREEAESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLEIEYLFVKEELRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAP
                     DFYQKHGYKEVFSLQDYLYIRQRYYYQKNL"
     gene            complement(2223..2705)
                     /gene="greA"
                     /locus_tag="VVE42_001052"
     CDS             complement(2223..2705)
                     /gene="greA"
                     /locus_tag="VVE42_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEU3073258.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
     gene            complement(2776..4431)
                     /gene="mltG"
                     /locus_tag="VVE42_001053"
     CDS             complement(2776..4431)
                     /gene="mltG"
                     /locus_tag="VVE42_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEU3073259.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSAEELMADSLSTVEEIMRKAPTVPTHPSQGVPASPADEIQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGPETPTPATETVDI
                     IRDTSRRSRREGAKPVKPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPI
                     DANSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQK
                     SMSTEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDQIAQTVGQLQGDFKESLTAEAF
                     LAKVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDE
                     MLAAMDKNLSPYYSTIKSKNLTVNELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDM
                     PLQSNIAILYAQGKLGQNISLAEDVAIDTNIDSPYNVYKNVGLMPGPVDSPSLDAIES
                     SINQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            complement(4512..5006)
                     /locus_tag="VVE42_001054"
     CDS             complement(4512..5006)
                     /locus_tag="VVE42_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU3073260.1"
                     /translation="MKIRQARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVGLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            complement(5003..5467)
                     /locus_tag="VVE42_001055"
     CDS             complement(5003..5467)
                     /locus_tag="VVE42_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU3073261.1"
                     /translation="MEIPIKIIQASKFDLPEIGALQTSSFPAEKQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQCLKVHSLVIESDHQRQGLGTLLLAALKEVAV
                     ELDYKGIRLESPDELLSYFEMNGFVDEEEILLYATSQGYSMIWFNPFYLEEQ"
     gene            complement(5477..6811)
                     /locus_tag="VVE42_001056"
     CDS             complement(5477..6811)
                     /locus_tag="VVE42_001056"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEU3073262.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFEAEGNDFVASDLLRSTTGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFAGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAVYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            complement(6822..7439)
                     /locus_tag="VVE42_001057"
     CDS             complement(6822..7439)
                     /locus_tag="VVE42_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEU3073263.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPITEEKPVKPIEKQVEKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQPTTPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSAPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            complement(7487..10585)
                     /locus_tag="VVE42_001058"
     CDS             complement(7487..10585)
                     /locus_tag="VVE42_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU3073264.1"
                     /translation="MAKLIPGKIRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLVFCACDFFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLDRILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINSQKYYVIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVSDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPNFQFDHQVDSYQTLLFQDMHADANLFAFTVTEYSDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMRLLKEIKALPVGQQGKKYLQFDSSDRDKLASC
                     LTLFIQMGTVSAPERLQIKTFAPSFYFDREEDNRIRLEIQFDYGNRQVSSRQELEELP
                     FSSDADLEERIFQVCLAAGFEADFQSWRQTLKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQDIAQEEIDQAMQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQARKSLAYSLAHLFEWRDRVSFSQEFQNLAQDL
                     THPEDFPRQATQVQADLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVAVVHGLKASREEILAESHQIYVTSY
                     ATFRQDSEFYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVVYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMEDYQ
                     GASGKLDSLRDLLLQVADGGHRVLIFSQFKGMLEKIEQELPDLGLTSFKITGSTPAKE
                     RQDMTKAFNQGERDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEA
                     ST"
     gene            complement(10678..11844)
                     /locus_tag="VVE42_001059"
     CDS             complement(10678..11844)
                     /locus_tag="VVE42_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU3073265.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IRQAVQTYADQLVYGYTYASEDLIKEVQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSVKLNNRRLITNSLVEKDGLFEIDFDQLEKDLVEEE
                     VKLYILCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVVDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNEAKVILNRGLDFGEEGSLHARINIAMPKSLLQEVCQRIVATFAKR"
     gene            complement(11852..12946)
                     /locus_tag="VVE42_001060"
     CDS             complement(11852..12946)
                     /locus_tag="VVE42_001060"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEU3073266.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVLATIESADYALATSSGMSAIVLAFSGFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGRFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHS
                     YGLTDDLLRLSIGIEDARDLIADLRQALEG"
     gene            complement(13103..13649)
                     /locus_tag="VVE42_001061"
                     /pseudo
     CDS             complement(13103..13649)
                     /locus_tag="VVE42_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866246.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(13685..14224)
                     /locus_tag="VVE42_001062"
     CDS             complement(13685..14224)
                     /locus_tag="VVE42_001062"
                     /inference="COORDINATES: protein motif:HMM:NF012869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073267.1"
                     /translation="MTEKIQEHELIKTNQAEKSVQDVLDNCIERVQNNSLKSDRVTSF
                     ETPFALLFIFATIAVMLTYGGYRVSAGYISVGTLVSFLIYLFQLLNPISNIANFVTVY
                     SRSKGSSVALDNLLAVPKEKFEGGKSVSGQGLNFNHVYFGYDENRPVLKDITCSIFKG
                     QKLLLLDHLDQENQRLCVC"
     gene            complement(14233..14732)
                     /locus_tag="VVE42_001063"
                     /pseudo
     CDS             complement(14233..14732)
                     /locus_tag="VVE42_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833286.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
BASE COUNT         4173 a         3282 c         2708 g         4569 t
ORIGIN      
        1 tacttagtgt agtcctaaaa tttaaatttg tcaactttta accgctattg gaagcgttta
       61 ataaaaatta tttccatttt gctaaatttc aagttcaagt tagccatcca gaagtcttct
      121 ctgggtgact tgtagtccaa gcatttttta ggatagttgt taatccactt ttcgatgaat
      181 gcgacttctt tgggagtcat tttcttggtt cccttaggta accatctacg aatgagcctg
      241 ttgtgattct cattagttcc cctttcccaa gaggcatagg gatgtgcata ataaatgtgc
      301 tcctcagaaa atacattaga caagcgattg aattccgttc cattatctgc cgtgatggaa
      361 agaatcttgt gttgttttaa gatgagtttt agagcctgat tgaccacctc agcactttta
      421 tttggaatca atcggatgat ctgatgtcta ctttttcgat ccgtcaagac aagcaagcag
      481 tagtttttcg ctctcgtaag tagaaccgta tcaatctcat aatgcccatt ctccaagcga
      541 aaattgatag cttcaggccg ctgttcgatg gattgaccag caggtttaaa gttggtgctg
      601 gcctgtttct taagcgcttt tccttttcta gggtaaagca gatcctgctt gctttacccc
      661 aattttccat gatgaatcca atagtaaatg gttgaaattc ccacgttaac ccctttagcc
      721 ataaccatca tttcaggcaa aaatttttgg ttatgatagt ggagaatctt ttcctttagt
      781 tccttggtca cgcttgatct cttgaccgag tgcttgcgat tattttcata agactgttga
      841 gcgtagtcgg cagaataaat ctctttgaag cgcccttttc caagacattt tcggactgtc
      901 ctacgcttga ttttagtgtg gatagtttga ggagcttttc caagtagaga ggcaatttct
      961 ctatttgatt ttccttcttt tttccatctt tcgattaagc gacggctatc gattgtcaaa
     1021 tgtttacctt ttgtagtata attgtcttgc atttctgtgc ctttcttgtg tttgtggttg
     1081 aacaacaagt ataacacaga ggtgttttct tatgcctaca agagctttca ttatttccat
     1141 tttcttttgg atttcactct attctgaaaa acttgtgtat atttactgaa gctagcaagt
     1201 cttacctgta aatttaatga aagcaacaca aaatccgaga ggggaatctc ggattaatag
     1261 atagagagtt tttagtttaa ataaattgtt taaaatatca acaacatcac ttcttttctt
     1321 aacctgataa gtcttgattc ctaattttgg ggctacgatt atattgtcct caatatcgtc
     1381 tagaaagaca caatttctag gttataactg gtatttatca atagttactc atatacatca
     1441 gtccacctcc atacttatgt gcgagcctct ctttgtatta tacctccata ctcaccttac
     1501 agattctttt ggtaataata tctttgccta atgtagagat agtcttgcaa agaaaaaact
     1561 tccttgtagc catgtttctg ataaaagtcc ggtgcctgga actggtaagt attgacaaag
     1621 gcaaaacaac aatttcgatt cttagcttca ctttctgcct gttgcaatag ttttgaaccg
     1681 attccttgcc ctcgcagttc ctcttttaca aacaaatact cgatttctag ccaatttcca
     1741 aaagtctctg ctatcaaacc tgccaggaga ttgccctttt catcttcgac ataaagatta
     1801 agtggctcac tttcagcctc ttctcttttt gaacggttat aaacacgaat cagattccct
     1861 atttcttgcg atttatgtga ttccttattt tccaatctaa agtatagtga aatgaaataa
     1921 aacatgcgca aatcgattaa ggaatttaat ctaatttcta acaatgtctt agaaatcaaa
     1981 gtgtactatt ttaacttcaa tgcactatac atctaatact caataaaaat caaagagcaa
     2041 actaggaaac tagccgcagg ttgctcaaaa cactgttttg aggttgtaga tgaaactgac
     2101 gaagtcagta accatacata cggcaaggcg acgctgacgt ggtttgaaga gattttcgaa
     2161 gagtagcaaa atggaaaaag gagtgagtga agcacatcgc ctccccactc ctttttctgt
     2221 ttttaggctg ttttttcaac cttcaagatt tttacatcat agctaccaac aggcgtttca
     2281 atggttgctg tatcacctgt tttcttgcca atcaaggcct gcccaattgg gctttcattt
     2341 gaaaccttac ctgcaaaggc atccgcacca gctgaaccta cgataatata aacttcttct
     2401 tcgtcctcac caatttcttg gatggtgact gttttaccaa tcgctacttc gtcctgggca
     2461 actgcgtcgc tattgacgat ttcagcatag cggatttttg tttctaagct agagatttgt
     2521 ccttcgacaa aggcttgttc atccttagct gcttcgtact cactgttttc tgaaaggtca
     2581 ccgtatgaac gggcaatctt aatgcgttct accacttctg gtcgacgaac caatttcaat
     2641 tcttctaatt ctttttcaag tttttccttt tcctcaaggg tcataggata tgttttttct
     2701 gccatttttc tcaactttct tctgataata ttttctaaag aaaattatgt gaagtatcac
     2761 ataattttag tttgtttagt ttaatttgct gttgacatgt tcagcgacat tgcggtcgtg
     2821 gtcttcttga ttgttagcat agtagacctt gccttctgtg acatctgcta caaagtagag
     2881 gttatcgctc ttagtttgat tgatgcttga ctcaatcgca tccagacttg gactatcgac
     2941 tggaccaggc atgagaccta catttttata aacattataa ggtgaatcaa tgttggtatc
     3001 aatcgcaaca tcctcagcta gactgatatt ttgccccagt tttccttggg catacaagat
     3061 tgcaatatta ctttgaagtg gcatatcacg attcaaacga ttgtagaata cacctgcaat
     3121 gagcttacga tcttctgtct tggcaccttc tttttcgacc aaggaagcaa tggtcaacaa
     3181 ctcattgaca gtcaagtttt tagatttgat agtactatag taaggagata ggttcttatc
     3241 catagcagct aacatctcat caatcaagct ctcaatagtt gtgctttcct tgatagagta
     3301 tgtagctggg aaaaggtatc cttccaaacg ataacgcgca ccgctgtctt ttacaggcaa
     3361 actttccagt aaagtaggat atttcgctac tgcttgactg ataaacgtct catcttgaac
     3421 tttagccaag aaagcctccg ctgtcaaaga ctctttgaag tcaccttgca attgacccac
     3481 agtttgagca atctgatcca aggtataacc ttctggaatt gtcaaagtcg caagtacagg
     3541 ttcttgcggt tcatctgttc cacctttttg caactctttg agtaagtctt ctgtactcat
     3601 actcttttgc aaattgtagt aacctgcttt caagtcggta taatttttat acttggcata
     3661 aaaactaaaa atcagaccat gctttaccaa accagctttt tcaagcgtcg taccgatttc
     3721 ttgaacgttt gaaccttctg gaattccaac cgtcacatat ttcttagaat tagcatcgat
     3781 aggtaataag gaatctagca cgtactggta accaaagtaa ccacctgctg agagcaaggc
     3841 aaggaataca aggaatgaaa tcacaaaagc tttcacatgt gacttcttct ctttcttagg
     3901 cttaacgggt tttgctcctt ctctacggct acgacgtgat gtatctctga tgatatccac
     3961 tgtttctgta gcaggcgtag gtgtttctgg acctgcttgt tctgcttttt tctctgccgt
     4021 cgtataggaa acagctaccc ttgttggggt ttcattgtat tctctttcaa gtttcttagg
     4081 tctaacagga cctggacctg gtcttgatcc actttcttcc gctggagaag aaggtacatc
     4141 ttgacttgga tgacttggaa caccaggagt ttctctttga atctcatctg ctggagaagc
     4201 tggtacacct tgacttgggt gagtaggcac ggtaggagct tttctcataa tctcctctac
     4261 cgttgacaag gaatcagcca tgagttcttc agctgaaggt tcatttgcag gagtgcgaac
     4321 tactgcctca tcttctttca gaacttcatc atagcctttt actttttcta aatctctcag
     4381 aatctgctct ttaaagctta atttctcttc ttctcttgac ttttcactca aaagtttttc
     4441 ctccttgttg ataatccata acattatatc ttaaaaatcc aaaaaaagca atctatgata
     4501 cttttcctaa cttatttttt catttcccag accatatcat accatgtttc ccctgcaaag
     4561 gttgactggg actggccttc gttgacaaat ccatgttttt cataataggc gatgagatag
     4621 tcatgacagg ttagattaat gccatctctc tcatcttcaa gagcaacttc tttcaaggct
     4681 gtcagcaatt tctgacccag tccgagtccc tgtgcctcct tggcaataga cagacaggtc
     4741 acagagatat aaccaccagg ctcatgacta tagtctttta tttcttctgt aaaagactgg
     4801 tcttgcagat ggcggtgcag gccaactggt ccttcgatat aacccatgat tcttccttct
     4861 ttttcagcaa ccagaaaaga ggtctgaatt tctctcaaat gtgcttcaaa gacagaagga
     4921 ggaatggctt cttcgaccga aaaattttca aattcaagtt caacaatccg atccaaatct
     4981 tctaatcttg cttgtctgat tttcattgtt cctccagata aaagggatta aaccaaatca
     5041 tactatagcc ctggctagtt gcatagagca aaatttcttc ttcatcaaca aaaccgttca
     5101 tttcaaaata ggaaagcagc tcatcaggac tctccaaacg aatccctttg taatccagct
     5161 caactgccac ctctttcaag gctgcaagaa gaagtgttcc caggccctgt ctctgatggt
     5221 cagactcgat gactaaagaa tgtactttta gacattgcgg attgtctgac tggggacttg
     5281 ataaaatata gcctaaaagt tgattttcat ccctagctag aagaaaggta tccgcacact
     5341 tacggatact ttcttctaaa atatgggaaa gttgctgctt ttcagctgga aaagacgagg
     5401 tctgaagtgc ccctatctca ggcaaatcaa acttgcttgc ctgaatgatc ttaattggaa
     5461 tttccatggg aacatcctat tgaacattgc ttgtcaagtt agacaagaga cgctcaaatg
     5521 agtattcata ggtttggatg tctcctgctc ccataaagac gtaaacagca ttgtcatggt
     5581 ctaggagtgg agaaacattt tcaacagtaa tcacttggtg ttttttgttg attttattgg
     5641 ctaggtcttc taccttaacg tcaccatgat ctacttcacg agccgagcca taaatttgcg
     5701 ctagataaac agcatctgct tggtttaaag catgggcaaa gtcgtccaac agggcaatgg
     5761 ttcttgtaaa ggtatgcggt tgaaagactg ctacaatttc cttgcttggg tatttctgac
     5821 gagccgcatc caaggtcgca ataatttctg ttggatgatg ggcaaagtca tcaataatca
     5881 ctgtatcatt gacaattttc tcagtgaaac gacgtttaac accggcaaat gttttcaagt
     5941 gctcacgcac caagttcaaa tcaaatcctg ctgtgtaaag aagaccaata acggctgtcg
     6001 cattcatgat attgtgacga ccaaaggttg gaatgtggaa ttgccccaag ttttgtccac
     6061 ggaaatgaac ggtgaaggtt gaaccagttg ttgaacgaag aagatcacta gctacaaagt
     6121 cattgccttc agcttcaaaa ccataataat aaattggtgc atcagacgta atcttacgca
     6181 attcagcatc ttcaccatag acaaaaagac ccttggtaat ttgtttggca tagtcgttaa
     6241 aggcattgaa aacatcctcg agacttgtga aataatctgg atggtcaaag tcaatgttgg
     6301 tgataataga gtattctggg tggtaaggca tgaagtgacg ctcatattcg tcagattcaa
     6361 agacaaaata tttggcatta gccgaaccac gacctgtccc atctccaatc aagaagctgg
     6421 tatctgtaat gtgagacaag acatgagaca acatacctgt cgttgaagtt tttccatgtg
     6481 ctcctgctac tcccatgcta acaaagtcac gcataaagct acctagaaac tcatggtaac
     6541 gtttgtagct gataccattt tggtccgcat aggcaatttc gacgttgtta tctggacgaa
     6601 aggcatttcc agcgataatt tccatatcac cgtctagatt cttttcatca aaaggaagaa
     6661 tggtaattcc tgcctgctca agaccgcgtt gggtaaagta gtacttttca acatctgatc
     6721 cctgaacctt gtgccccatc tggtgcaaca tcaaggccaa ggcactcatc cctgatccct
     6781 taattccgat aaaatgatat gtctttgaca tgttttctcc cctattctgt cattctggtc
     6841 agattcaact cttgggcaac ccgacgttct tgttctgttt gtttactttt tttattgtag
     6901 atttggctct tctttagaaa atcataattg tttttctttg gagcaggtgc tgacacttct
     6961 tcattcttgg tagggataga atgaacttct tccgccaaga tataatgaga ctgggtcaat
     7021 ttttggctat atttgacaaa ttcaccagga ttttcctttt ggaaaggagt tgtcggttga
     7081 ttgccctgtc taactagact gggctgagaa tgacgtctcg caaggctgaa atcctgagtt
     7141 aggtagttag cagagcgttt ctttttcaag tccgcacgcg cttcttcacg cgccacctcc
     7201 gcatagctct ttccttcttt tttaacccct aaaggagcct ttttaggttt ttcgacttgc
     7261 ttttcaatcg gttttactgg tttttcttca gtaataggag cccattctaa ataattttta
     7321 tctcgatact cacccttgat attactgatc agatcagact catcatagag attcatgact
     7381 ggcatttcag tcaacatgac ctcgtcatct gacaccaatg gaaatcgttc ttgtttcatt
     7441 ttctatttcc tttcaacact tcattatagc gtattgtctt gatttttcaa gtgctggctt
     7501 cagaaattcc caaaatttct ctaatttctg ctagggtcag actaccacgt gactctgtgc
     7561 catccaatac ttgtgacacc agatgtttct tttgttcttg gagttcctga attttttctt
     7621 caatggttcc cttggtcacc aagcgataga cctcaaccgt ttcttcctga cccatccgat
     7681 gggcacggcc aatggcttgc gcttccaccg caggattcca ccaaaggtca accaagatca
     7741 ctgtatctgc acctgtcagg ttcagaccga ccccaccagc cttgagggaa atcagaaagg
     7801 catctctttc tccttggtta aaggccttgg tcatgtcttg tctttccttg gctggggttg
     7861 aacccgtaat tttaaaggat gtcaggccca agtctggcag ttcttgttca attttttcca
     7921 acattccctt gaactgagag aaaatcaaga cacggtgtcc gccgtctgcc acctgtagca
     7981 gtaggtctcg gagactatct agtttgccgc tggctccctg ataatcttcc ataaacaggg
     8041 caggagtgtc acatatttga cgcaagcgca tcaaaccaga taaaatttcc acacgacttc
     8101 gctgaaattc ctgttctgac acttgagcca gatggtctcg catctgttgt aactgggcaa
     8161 ggtaaatagc cttttgctgg tcttccagtt catttttata aaccacctca atcaagtctg
     8221 gcaattcagt cagaacttct tctttcttgc gtcgcatcac gaaaggcttg ataaactgag
     8281 ccactcgctc tgctggcaat ttcataaatt ctttcttgct tggcaaaagt ccgggcatga
     8341 cgatttggaa aatagaccac aactcaccca gatggttttc aatcggagtt cctgacaagg
     8401 caaagaccga cggcaccaca aattgtctca aggtctgggc aatcttggtc tgggcatttt
     8461 tcatgacctg agcctcatct aagaaaagga agtcaaaggc catcccctga taaaactcac
     8521 tgtcctgacg gaaggtggca tagctagtca catagatttg atggctctcg gcaagaatct
     8581 cctcacgact tgctttcaaa ccatgaacaa cagccacatc caactgtggg gcaaatttct
     8641 gaaactcatc tgcccagttg taaatcaaac ctgacggagc gagaatcaaa acccgacttt
     8701 cttttgtcac ttgactagtc aaaaaagcaa tggtctgaag ggttttccca agtcccatat
     8761 catcagccaa aatcccacca aaaccataat gatggagcat ctgcaaccag ccaattccct
     8821 tttcctgata atctcgcaag tcagcctgaa cctgagttgc ctgtcgagga aagtcctctg
     8881 gatgtgtcaa atcctgggcc agattctgga attcttgtga aaaagaaaca cggtctcgcc
     8941 attcaaagag atgagctaaa ctgtaggcca aggatttccg agcctgcaag gtcccatctt
     9001 ttaattcaaa ttgccccagt tcctgtagat tttggcgaat tttcttggtt tcttcatcga
     9061 aaaagtaaac ttgattagac gaatcaatat aaaaatcctg attggcaacc aaggcctgca
     9121 tagcttggtc gatttcctcc tgggcaatat cttgaaaatc aaactggatt tccaagagac
     9181 ctcccttgga ggcaatctgc acctgaggac tcgctaggct ataaagctct tctagtttat
     9241 ctgataggtc aacatgcccg agtttttcaa agactggaat gatatcatga aaaaaatgat
     9301 agacagactc cgcttttaag gtctgacgcc aagattgaaa atcggcttca aagcccgcag
     9361 ccaaacagac ttggaaaatt ctttcttcta agtccgcgtc acttgaaaat ggtaattctt
     9421 ctagctcttg tcggctagat acctgtctat ttccataatc aaactgaatt tctaaacgaa
     9481 tccgattatc ttcttccctg tcaaagtaaa aagagggcgc aaaagttttg atttgtagac
     9541 gttctggagc tgaaacggtg cccatctgga taaaaagagt cagacaggag gccaatttgt
     9601 ctcgatcact gctatcaaat tgcaggtatt tctttccttg ttgacccaca ggtaacgctt
     9661 taatttcctt gagaagacgc atctgctggt ctgttaaaaa ataaacctga cctttatgga
     9721 aaagtactgc tccctgataa aagacattga ccctaggact ctcactgatt tccatttcaa
     9781 aatagtccga gtattctgtt actgtaaagg caaatagatt ggcatcagca tgcatatcct
     9841 gaaaaagcag ggtttggtag ctatccactt gatggtcaaa ttgaaaattg ggcaaggcca
     9901 tcagtaaatt cacaccctgc tcaaaaaagg tcagagggaa aaagaggtgc cgaccttggt
     9961 tttggaaaaa gaggtctgaa accagccctt cctccgttag tccgtgcaag aaagtcaaaa
    10021 gttcttggct ggcatcatca aaggcttccc aagaaagaga ctcctcataa atcttgccaa
    10081 tcatatacga ctttctctgc tcgacaatcc ttaaaaaaag tggaatatcc cgaatgacat
    10141 agtatttttg gctattgatt tggccgattc tcagagtcca caagatatga ttggttcctg
    10201 cttccacctg acccacagct gataactcat aggcgcattc tgattttgga gataaaattc
    10261 gatccaaaaa cttgccaccc aaggttacct tggtttcaac agcctctttt tcttcatgac
    10321 cttcttccag actccacaag atttcctgac cacgctcatc atttttcaga aaatgctcta
    10381 gcgctgccaa atgcacacag taacccctct tttgaaaaaa atcacaggca caaaaaacca
    10441 aatcatcctc taaactatag cgcagttctt cttctgcaac gcgagcgtag agccgattgt
    10501 tcttttcctt gatgatatca accttaccag tttcataaag ggcaacacct tcgatacgaa
    10561 ttttccccgg aatcaattta gccatatttt cacctttacc ttatcttttt attataccat
    10621 attttcgcct atgaaaatag ccttctagga agacttttct cctagaaggc tggattttta
    10681 acgtttggca aaagtagcca caatccgctg acagacttct tgcaacagag atttgggcat
    10741 agctatattg atgcgggcat ggagacttcc ttcctctcca aaatccaaac cacggttgag
    10801 gataaccttg gcttcatttc tcaacaactc ttgcaatgtt tcatcagtca ggtcataagc
    10861 tgaaaagtca agccaaatca agtaggtacc ttgcggtttc atgaccttga ttttagtctc
    10921 ttttccaaat agatccacca cataattgat gtggtcttca aagacttgct tgagttcctc
    10981 tagccaatct ttaccgtatc gataggcagc ttctgtcgcc aaataaccca agcctgaaat
    11041 ttcatgctga ttattggcca acaggcgttt ctggaaagct agtctcaact taggattttc
    11101 aatgactgca taggaatttt ttgttccagc aatattaaat gttttagtgg cactgctcaa
    11161 gacgatagca aaatttttga aggcaggatt gatggtattg aaagactggt gtttgtgacc
    11221 aaagagggtc aaatcttggt gaatctcatc cgaaactaac aaaacaccgt gtttttggca
    11281 gagttggcca atcttctcca acacttcttt ttcccaaaca cgtccaccag gattgtgagg
    11341 gttgcaaaga atatagagtt taacctcctc ttccaccaaa tccttttcaa gttggtcaaa
    11401 gtcaatctca aacagaccat ccttttccac taaggaatta gtaatcaatc tacgattatt
    11461 caacttgaca ctgcgagcaa agggtgggta gacaggcgtg ttaattaaaa ccgcctcgcc
    11521 ttcttttgta aaggtttgaa tagctgttga gatggctggt accacaccct cgataaagac
    11581 aagagcctct ttgtcaaagt tgtaaccgta ttgtgtagct tcccactttt gaacttcctt
    11641 aattaagtct tcactggcat aggtataacc ataaaccagt tggtctgcgt aagtttgcac
    11701 ggcttggcgg atttcaggca agaccacaaa gtccatatcc gctatccaag ctggtagaac
    11761 ttcactatcc gtttctgttt ctttccattt ataggtatgg tgccctaaac ggttgggcag
    11821 gcttgtaaaa tcatattttc ccatctttgt cttatccttc taaggcttgg cgcaaatctg
    11881 caatcaaatc tctagcatcc tcaatcccaa tagacaaacg caagaggtca tctgtcaaac
    11941 cataagaatg gcgtacttct gctggaatat cagcatgagt ttgagtcgtt ggataagtaa
    12001 taagactttc cactccgccc aaactttccg caaaagagaa gaccttgaga ctgttcaaaa
    12061 tatgaggaat gcgtgtttca tcggctactt taaaggaaat catgcctcca cgaccagtgt
    12121 agagaacttc cttaactgct ggagaatcct tcaaaaaggc aaccacttct tgggcgttag
    12181 ctgttgagcg ctccatacga agagacaagg tcttgagacc acgaagcaac tggtagctgt
    12241 caaatggaga caagactgcc cctgttgtat tgagattgta aaaaagcttc tcgtatagtt
    12301 ctaaactatt ggtcacaacc actccagcca agacatcatt gtggcctgct agatacttgg
    12361 ttgctgaatg gagaacgata tctgctccat cttcaatcgg acgttggtag atagggctat
    12421 agaaggtatt gtccaccacc actttggcac ccttagcatg agccaatttt gctagttttt
    12481 cgatatcaaa ttccaacatc aagggattgg ttggggtttc gatatagaga acatccacat
    12541 ccttttctaa ctcggcaatc aactcttctt ctgtattggc ataggtaaaa tggaaacgac
    12601 cttcctgctc cacttggtta aaccagcgaa aagaaccgcc gtaaagatca cgcactgcca
    12661 agaccttact tcctactgga aagccgctaa aggccagtac aatagctgac atccctgagc
    12721 tagtcgctaa ggcatagtct gctgactcaa tagtcgccaa gacttcctca gccttactac
    12781 gagttggatt tttagtgcgc gtatagtcaa acccagtaga tcgaccaaac tctggatgct
    12841 gataggtcgt tgaaaaatga agcggtgtca ccaatgcacc tgtcgcttca tctgacttaa
    12901 tacccgcctg ggccaaaatt gtgttaatgt gtaattcctt gctcatacaa tacctccaaa
    12961 tctatagtaa ctattgtacc acttattttc atcaactcat tttcttcttt tcaagagcta
    13021 gttatagttt caaactatat aaaaagggag tttttcctgc tccctttaat agactataaa
    13081 atggtgaatc tcaaaagaca cttcactcta tcatttgctc ctgcacaaaa cgagcataac
    13141 gctcatgatt ttccagtagt tccttatgag ttcctgagcc agtgattttc ccctcctcta
    13201 agaagaaaat acaatccaca tcttttaccg ttgacaaact atgcgctata atcacaaccg
    13261 tcttctcctt taatacagaa tagaggctac tgataatcgc atactcagaa tccgcatcaa
    13321 gattagcagt agcttcatca aatataagaa tttcagcatc ttttaagtag gttctagcta
    13381 tttgaagtct ttggctttgc cccccctgac aagaatcgtc cgcgttcacc aacttcagta
    13441 tctagtccct ctttcatgga gcgaatctca tcacctagtg atactaagtc tagcactttc
    13501 atcaattcat catcagttac taagcgattc aaaccgagac aaagattgtc acgaatactg
    13561 ccagataaga ctgcattatt ttgtgaaacc caagcgattt tacttctcca ttcttttaag
    13621 ttaaaatcat atatacttga ttgctccatt agaatatctc ctgaaagcgg tttataaaac
    13681 cgctctaaca aacgcacaat cgttgatttt cctgatccag atggtccaac aaaagcaatt
    13741 tttgcccctt gaaaattgaa caagtaatat cctttaagac aggtcgattt tcatcataac
    13801 caaaatagac atggttaaaa ttcaaccctt gtcctgatac cgattttcct ccctcaaatt
    13861 tttctttagg aactgcaagc aagttatcca gtgcaactga agatcccttg ctcctagaat
    13921 aaacagttac aaaattagct atattactaa taggattaag taattgaaag aggtaaatca
    13981 aaaacgaaac caaggttccc acagatatat atcctgcgct gacccgataa cccccatagg
    14041 ttagcatcac agctatagtc gcaaagataa ataagagagc aaacggggtc tcaaaagaag
    14101 taaccctatc tgatttcagt gaattgtttt gtaccctttc aatacaatta tccaaaacat
    14161 cctgtacact tttctctgct tggttagtct taattaattc atgttcttga atcttttcag
    14221 tcaattgccc tgttaaattt cctcctgtaa acgacgacta tacttttcac tgatattgga
    14281 aaggggcaag ataataaaca tcatacaagg aagagtgatg aataaaacta gagaaagatt
    14341 ccaatcaaga ctaaataaga ctacaatgga accaagtacc ataactaaac tcagaataat
    14401 atttgggaaa gtcgtaatta aaaactcacg aatgacactc atgtcattga caatggcaga
    14461 agtcaactcc ccactttggc tcttatcaaa gaaggatttc tctgcataaa tcaacccctc
    14521 tatcactttt ttcctgattt ttgctatctt tttttcaccc gattgactaa acagatagta
    14581 accaatagaa gaaaacaagg cttgaccaat acgattgaaa tactttggaa cctatatttt
    14641 caatagaact cccatctatt aaacccttta agataagggg aagcaacaaa gcaagtagac
    14701 tagacagaac aagtaagaaa ctccccataa tc
//
LOCUS       DAVGJL010000044        14648 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.044, whole genome shotgun sequence.
ACCESSION   DAVGJL010000044 DAVGJL010000000
VERSION     DAVGJL010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14648
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.044"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            66..1787
                     /gene="pabB"
                     /locus_tag="VVE42_001064"
     CDS             66..1787
                     /gene="pabB"
                     /locus_tag="VVE42_001064"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEU3073268.1"
                     /translation="MHRKTVIDFRVLGERYTFTQPIKEIKTRNVAEVADLLAQVESYQ
                     KQGYYVVGYVSYEAAPAFEEKLAVHKDPLLGEYLLYFTVHDSVETSPIPLTYEDIDLP
                     SNWQEVTSAADYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLNANPFAIYNRMVVEQEA
                     GYNAYVEHDEMAVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLEQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTVWQMTSTIKSQLREDVDLV
                     EIFRSLFPCGSITGAPKIATMEIIKNLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTIQ
                     LHKGQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGEISQKNLLFED
                     QHLERLRKASRYFAFPFDAEDLGHKIEEECQDCEANQDYRLRISLSKSGEIEVNRQVL
                     PPLSTSFCQAQVCLQEAALNQSFTYFKTTHRPHLSLGEQEKIYHNKSGELLETSIGNL
                     VLKIAGKLYTPPIRLGILPGIYRQYLLETGQVEEKVLTLTDFAQAEAIYGCNAVRGLY
                     ELSLEKN"
     gene            1945..2694
                     /locus_tag="VVE42_001065"
     CDS             1945..2694
                     /locus_tag="VVE42_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU3073269.1"
                     /translation="MNKRLFLKMSLVTLPILALFSQSVLAEENIHFSSCKEAWANGYS
                     DIHEGEPGYSAKLDRDHDGVACELKNAPKGAFKAKQSTVAQTNTSSATTSGWVEQDGA
                     WYYFDENGNPVKNAWQGNYYLKSDGKMAQGEWIYDSSYQAWYYLTSDGSYAYSTWQGN
                     YYLKSDGKMAVNEWVDGGRYYVGADGVWKEGQASTASSSNDSNSEYSAALGKAKSYNS
                     LFHMSKKRMYRQLTSDFDKFSNDAAQYAIDH"
     gene            2830..3789
                     /locus_tag="VVE42_001066"
     CDS             2830..3789
                     /locus_tag="VVE42_001066"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEU3073270.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTAGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDREKGTVIGAYNLNWKTLQPIKQKIE
                     KALGIPFFIDNDANVAALGERWMGAGDNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPIACTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFELAKEGDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENSFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            3886..4725
                     /locus_tag="VVE42_001067"
     CDS             3886..4725
                     /locus_tag="VVE42_001067"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEU3073271.1"
                     /translation="MTKADTIFKENIERILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPITTLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(4773..4943)
                     /locus_tag="VVE42_001068"
     CDS             complement(4773..4943)
                     /locus_tag="VVE42_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEU3073272.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            5064..5948
                     /gene="miaA"
                     /locus_tag="VVE42_001069"
     CDS             5064..5948
                     /gene="miaA"
                     /locus_tag="VVE42_001069"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEU3073273.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKRFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVSHHLIDVREITESYSAFDFVSEAKMTIEDIHSRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVDQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQETLYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRGEQTLEEASESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            5941..7179
                     /gene="hflX"
                     /locus_tag="VVE42_001070"
     CDS             5941..7179
                     /gene="hflX"
                     /locus_tag="VVE42_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEU3073274.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMEELASLAKTAGAVVVD
                     SYRQKREKYDSKTFVGSGKLEEIALMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESSTFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDLEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDG
                     EVITGYISEKNKWRLEEFYD"
     gene            7172..7795
                     /locus_tag="VVE42_001071"
     CDS             7172..7795
                     /locus_tag="VVE42_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEU3073275.1"
                     /translation="MTDIKTLALKYGGYTSLDKVYLDQLLAGKTEQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKPFIRLNLSGKSFGFCY
                     ESDGLGRIFSENEEKISDDLLFEIAQIFPHQLVFEESGKIYMKPVEDEEVVSLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            7810..8739
                     /locus_tag="VVE42_001072"
     CDS             7810..8739
                     /locus_tag="VVE42_001072"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEU3073276.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTIRPRKVSKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAEKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTDASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            8760..9515
                     /locus_tag="VVE42_001073"
     CDS             8760..9515
                     /locus_tag="VVE42_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU3073277.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEADAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLAGETFNYK"
     gene            complement(9569..10477)
                     /locus_tag="VVE42_001074"
     CDS             complement(9569..10477)
                     /locus_tag="VVE42_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU3073278.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLAHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            10541..10810
                     /locus_tag="VVE42_001075"
     CDS             10541..10810
                     /locus_tag="VVE42_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEU3073279.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            10810..11190
                     /locus_tag="VVE42_001076"
     CDS             10810..11190
                     /locus_tag="VVE42_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU3073280.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQ
                     LIDLRDPAEFHRKHILGARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
     gene            11279..11506
                     /locus_tag="VVE42_001077"
     CDS             11279..11506
                     /locus_tag="VVE42_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073281.1"
                     /translation="MKEKKKNPLFVGILSIILGLLFPIVGLILGIIGLVLAISYQKES
                     QLDYKIEKILNILGIVISVVNWIVAIALIFR"
     gene            complement(11546..12271)
                     /locus_tag="VVE42_001078"
     CDS             complement(11546..12271)
                     /locus_tag="VVE42_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HEU3073282.1"
                     /translation="MRLDNLLAQEKISRKAMKQALLRGEILVDGCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLD
                     RDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVEVNGLLTPDHIQAFQKGIVFLDGTVC
                     KPAKLEILSASPSISQASITISEGKFHQIKKMFLSVGVKVTSLKRIQFGDFTLNPDLA
                     EGNYRPLNQKELQIIKNYLEMSR"
     gene            12433..14274
                     /gene="typA"
                     /locus_tag="VVE42_001079"
     CDS             12433..14274
                     /gene="typA"
                     /locus_tag="VVE42_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEU3073283.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPVEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGVKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            14294..14548
                     /locus_tag="VVE42_001080"
     CDS             14294..14548
                     /locus_tag="VVE42_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEU3073284.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLILAYFSVRDITLMPVKKSKRR"
BASE COUNT         4303 a         2816 c         3368 g         4161 t
ORIGIN      
        1 aaatagatga gtaaaaaggt agattaagtt tttcttgatc tgccttttta tgctatactt
       61 aaggtatgca tagaaaaaca gtgattgatt ttagggtttt gggggagaga tacaccttta
      121 cccagcctat caaagagata aaaacgagaa atgtagcaga agtggcagac ttgctggcac
      181 aagtggaaag ctaccaaaag caaggctatt atgtggtggg gtatgtcagc tacgaggctg
      241 cacctgcttt tgaggaaaaa ttagcagttc acaaggatcc cctactggga gagtacctgc
      301 tttactttac tgttcatgat agcgtagaaa cttcccctat tcctctgact tatgaggata
      361 ttgatttgcc ctcaaattgg caggaagtaa cgtctgcagc agactatgaa aaggccattg
      421 cccaaattca ccatcattta cggcagggag atacctatca ggtcaactac accgtccaac
      481 ttaagcaaaa gttaaatgcc aatccttttg ccatctacaa tcgtatggtg gtagagcagg
      541 aggcgggcta caatgcctat gtggaacatg acgagatggc agtgatttcc atgagcccag
      601 agctcttttt tgagcaaaat gatcgcgagt tgacaacacg accaatgaag gggacgactc
      661 agcgtggggt aactgaccaa gaagatcttg aacaggccag ttggttggaa caggatccca
      721 aaaatcgctc tgaaaatatg atgattgtgg acctcttgcg caatgatatg aaccgtattt
      781 ctgaagttgg gagcgagcac gtggagcgtc tgtgccaggt agagcagtat tcaactgttt
      841 ggcagatgac ttcgaccatc aagagtcagt tgcgagagga tgtggacctt gttgaaatct
      901 tccgctcact ctttccttgt ggttccataa cgggtgcacc gaaaattgcg acaatggaga
      961 ttatcaagaa cttggagcct caaccgcgtg gagtctactg tggaacgatt ggtctcttgc
     1021 ttccaaatgg acgacggatt tttaatgtgg ccattcgtac cattcaactt cacaaaggtc
     1081 aagccattta tggagttggc ggagggatta cttgggatag cacatgggaa tctgaatacc
     1141 gagaggttca tcaaaaggca gcagttcttt atcgtaaaca agctcgcttc caactgatta
     1201 cgacagggga aatcagccag aagaacctgc tgtttgaaga tcaacatctg gaaagactga
     1261 gaaaagctag tcgttatttt gcctttcctt ttgatgcaga agacttggga cacaagattg
     1321 aggaagagtg tcaggattgt gaagctaatc aagattaccg cttgcgaatc agccttagca
     1381 aatctggaga gatagaagtc aatcgtcaag tattaccccc tctcagtaca agcttttgtc
     1441 aggcccaagt ctgccttcag gaagctgctt tgaatcaatc ctttacttac tttaaaacca
     1501 ctcaccgacc gcatttgagc ctaggagaac aagagaagat ttaccacaat aagtcaggag
     1561 aactgcttga aacctctatc ggaaatttgg ttttgaaaat tgctgggaaa ctctacacac
     1621 cgcctatcag acttggtatc ttgccaggaa tttatcgcca gtatttacta gaaacaggac
     1681 aagtagaaga gaaagtcttg accttgacag attttgccca agcagaagct atttatggct
     1741 gtaatgcagt gagaggcttg tatgaattaa gcctagagaa aaattagatg aaactggtat
     1801 ttttacacgg tttaggggag tcagcagagt cttggaaaga agttcggaat caattgaagg
     1861 ataagcccac agagactctt gaattgctga aataagtttg ataaaataat attgaaatcg
     1921 atatcataat tatgggagaa agaaatgaat aaacgtctat ttttaaaaat gagtctggtg
     1981 acgttgccaa ttttagcctt gttttcacaa tcagttttgg cggaagaaaa catccatttt
     2041 tcgagctgta aggaagcttg ggcaaatgga tactcggata ttcacgaggg agaaccggga
     2101 tattctgcca agttagaccg agaccatgat ggtgtagctt gcgaattgaa aaatgctcct
     2161 aagggtgctt tcaaagcaaa acaatcaacg gtggctcaaa ctaacacaag ttcagcaaca
     2221 acaagtggtt gggttgagca ggatggcgct tggtactatt ttgatgaaaa tggaaatcca
     2281 gtgaaaaatg catggcaggg aaactattat ttgaaatcag atggtaaaat ggcacagggc
     2341 gaatggattt atgattcttc ctatcaagct tggtattatt tgacatcaga tggttcttat
     2401 gcttacagta catggcaagg aaattactat ctaaaatcgg atggtaaaat ggctgtcaat
     2461 gaatgggttg atggtggacg ttattatgtt ggcgctgacg gagtttggaa ggaaggtcaa
     2521 gcaagtacag cttcttctag taatgatagc aatagtgaat attctgctgc tttaggaaag
     2581 gcaaaaagtt ataattcgtt attccacatg tcaaaaaaac gtatgtatag acaattaact
     2641 tctgattttg ataaattttc aaatgatgca gctcaatatg ccattgatca ttagatgatt
     2701 aacagatact ggtaggcgaa aaaaattcct tataattcac ttacgcaaac ctttgcataa
     2761 gtgaatgaat tttgatatac tagtactgta agcattttca attactacac aatgaaaaag
     2821 gagaatatga tgagtcaaaa gattattggg attgaccttg gtggaacttc tatcaaattt
     2881 gcaatcttaa caacagcagg agaaatccaa ggaaaatggt caatcaagac caacattttg
     2941 gatgagggaa gtcatatcgt tgatgatatg attgagtcta ttcaacatcg tttggacttg
     3001 cttggattgg cagcagcgga cttccaaggc attggaatgg gatcaccagg tgtggttgac
     3061 cgtgaaaaag ggactgttat cggtgcctac aacttgaact ggaaaaccct tcaaccaatc
     3121 aaacaaaaga ttgaaaaagc cttgggcatt ccatttttca tcgataatga tgccaacgta
     3181 gcagctcttg gtgagcgctg gatgggtgct ggagataacc aaccagacgt tgtctttatg
     3241 acactcggta ctggtgttgg tggcggtatc gtcgcagaag gcaaattgct tcacggtgtt
     3301 gctggtgcag caggtgagtt gggccacatc actgttgact ttgaccagcc aattgcatgt
     3361 acttgcggta agaaaggctg ccttgagaca gttgcttcag caacagggat tgtcaacttg
     3421 actcgtcgct atgccgatga atacgaaggc gatgcagcct tgaaacgctt gattgataac
     3481 ggagaagaag taactgctaa gactgtcttt gagctcgcaa aagaaggaga cgaccttgct
     3541 ttgattgttt accgtaactt ctcacgttac ttgggaatcg cttgtgctaa cattggttca
     3601 atcctaaacc catcaacaat cgtcatcggt ggtggtgtgt cagctgcggg agaattcctt
     3661 ctacaaggtg ttcaaaaagt ttacgatgaa aatagtttcc cacaagtacg cacatctact
     3721 aaattagctc ttgcaactct aggaaatgac gctggagtta tcggagcagc atcacttgta
     3781 ttgcaataag ataatgagag aggaaaatgc taacctagag ttagtaatgt tcctctttta
     3841 ttttttatgc tatactagtt aggacaatga atgaggtgaa acgaaatgac aaaagcagat
     3901 acgattttta aagagaatat tgaacgcatt ctaaaggaag gtgttttctc agagcaggct
     3961 cgtcctaagt acaaggatgg gactgttgct aattccaagt atgtaactgg tgccttttcc
     4021 gagtacgact tatctaaagg agaatttcct atcacaacct tgcgtcccat tgcaatcaaa
     4081 tccgccatca aagaagttct ctggatttac caagaccagt caaatagctt agaagtgctt
     4141 aatgacaagt acaatgttca ctactggaat gactgggaag ttggagacac gggaaccatt
     4201 ggtgagcgct atggtgccgt tgttaagaaa cacgacatta tcaataagct tctcaaacag
     4261 ttggaaacca atccttggaa ccgccgcaat attatttcgc tctgggatta ccaagctttc
     4321 gaagaaacag atggactgct cccgtgcgcc tttcagacca tgtttgatgt tcggcgtgtt
     4381 gatggggaaa tctatctgga tgcgaccttg acccagcgct ccaatgatat gctggtggcc
     4441 caccacatca acgctatgca gtatgtggct ttgcagatga tgattgccaa acattttggc
     4501 tggaaggttg ggaagttctt ctacttcatc aacaacctcc atatctatga taatcaattt
     4561 gaacaagctc aggaattgct ccgtcgggag ccgtcaaact gccaaccacg cttggtttta
     4621 aatgttcctg atgggactaa tttctttgat atcaaagcag aagattttga gttggtggat
     4681 tatgaccctg ttaagccaca gttgaagttt gacctagcta tttaaaagaa tagaaaaaag
     4741 aagttgagaa taatcccaac ttcttttgtt tcttaacgtg atacgcggcg acgagctgct
     4801 tttttacggt tttcttcgat gaaagctgct ttttgctctt ctggttcgat tactttcttt
     4861 ttaaatgcgt atactgcacc tgcaacggca gcgacagttc ctgcgacacc tgttacaaga
     4921 cctttagcga atcctttagc catgagtctt cctcctttat attctcaatc agccagcctc
     4981 ctcaagaggt cacatttttc tgactgacct ttttgtgtta taataatagt aacgaaaaaa
     5041 tgggaatttt tcaaggaaaa aagatgaaaa caaaaataat tgtgattgtt ggaccgactg
     5101 ctgttggaaa gacggctcta gctattgaag ttgcaaagcg ttttaatggc gaagtggtta
     5161 gtggagatag ccaacaagtc tatcgaggac ttgatattgg gacggccaag gctagtccag
     5221 aagagcaggc agctgtttct catcatttaa tcgatgttag agagataacc gagtcttact
     5281 cggcttttga ttttgtttca gaagctaaga tgactattga ggatattcac agccgtggca
     5341 agctagccat tatcgccggt gggactggac tttatatcca gagcttgcta gaaggttacc
     5401 acctaggtgg ggagactcct catgaggaga ttttagctta tcgagctagt ttggagccat
     5461 attcagatga ggaattagcc catttggtgg atcaagcagg ccttgagatt ccccagttta
     5521 atcgtcgtcg tgctatgcgt gccttagaaa ttgcccattt tggtcaggat ttggaaaatc
     5581 aagagacctt gtatgaaccg ctgattatct gcttggatga tgaacgtagt caactttatg
     5641 agcgtatcaa ccaccgagtg gatttgatgt ttgaggctgg gcttttggat gaggccaagt
     5701 ggctgtttga ccattcccct aatgtacagg ctgctaaagg aattggctat aaggaactct
     5761 ttccttattt ccgtggagag cagaccttgg aggaagctag tgagagtctt aaacaggcga
     5821 cccgtcgttt tgccaagcgt cagctgacct ggttccgtaa tcgcatgcag gtcacctttt
     5881 atcagattgg agaatctggt gtacaagacc gcattttaag ccagatagag gagtttttag
     5941 atgattgaaa cggagaaaaa agaggagcga gttctgctga ttggtgtgga attgcagggt
     6001 atggacagtt ttgacctctc catggaagaa ttggctagtt tagcgaaaac ggcaggggca
     6061 gtcgttgtag atagctacag acaaaaacgt gaaaaatatg attccaagac cttcgtcggc
     6121 tctggtaagt tggaagagat tgcgcttatg gtggatgcag aagaaatcac tactgtcatc
     6181 gtcaacaacc gtctgacccc aaggcagaat gtcaatctag aggaagttct cggtgttaag
     6241 gtcattgacc gtatgcagtt gattttggat atctttgcca tgcgggctcg aagccatgaa
     6301 gggaagctcc aagtccacct agcccaactc aaatacctct tgcctcgctt ggttggtcag
     6361 gggattatgc tcagccgtca ggcaggggga attggttccc gtggtcctgg tgaaagccaa
     6421 ctggaactga accgtcgtag cgttcgcaat caaatcacgg atatcgagcg ccagctcaag
     6481 gtggttgaga aaaatcgggc gactgtcaga gaaaaacgtt tggagtctag cacttttaag
     6541 attggtttga ttggttatac caatgctggg aaatcaacta tcatgaacat cttgaccagt
     6601 aagacccagt atgaagcaga tgagctcttt gcgactctgg atgcgacaac caagagtatt
     6661 catctgggag gcaacctcca agtaactttg acagataccg ttggctttat ccaagatttg
     6721 ccgacagagt tggtgtccag tttcaagtca accttggaag aaagcaagca tgtggacctt
     6781 ctggttcatg ttatcgatgc tagcaatcct taccacgagg aacatgaaaa aacggttctc
     6841 tccatcatga aagacctgga cctggaagat attcctcact tgacgcttta taataaagcg
     6901 gatttggtgg aggatttcac gcctacccaa acgccatata ccctcatttc tgccaagtct
     6961 gaggacagtc gtgaaaactt gcaagcatta ttgctagata agattaagga aatttttgaa
     7021 gcatttaccc tgcgagtgcc tttttcaaag tcctacaaga ttcatgattt agagagtgtt
     7081 gcaattctgg aagaacgtga ttatcaggaa gacggcgaag tgattacagg ctacatttcc
     7141 gagaaaaata aatggaggtt agaagaattt tatgactgat attaaaactt tggctctaaa
     7201 gtatggaggt tatacaagtc tggacaaggt ctatctggat cagcttctag ctggtaaaac
     7261 agagcaggag cagttaacac ttattacacc tccgccaagt gttgttaatg cctactttgc
     7321 agaactctat cagaaaaaaa gtcctcaggc tgcgacggat tattttgcag aactcagtca
     7381 ggaactgaat ctttacaata ctgagccaag tttcacccta gaaaataagc cttttattcg
     7441 gcttaatctg tctggtaaat cctttggttt ttgttatgag agtgacggcc ttggtcggat
     7501 tttctctgaa aatgaggaga aaatctcgga tgacttgctc tttgaaattg cgcaaatttt
     7561 cccccatcaa ttagtctttg aagagtctgg taagatttac atgaagcctg tcgaggacga
     7621 ggaagttgtc agcctagaaa gtctcacagc tttgactgat ttggaaagat tggctgatgg
     7681 tcgaaaacgt ctcaaaggct acagccaaga ggatttattg caagaagctg ctgctttttc
     7741 tggcaaacgc tatttccgat cggaaaaccg cacagccatg ttatatattg attaattaga
     7801 aagtatcgaa tggatattca atttttagga acgggggctg gtcagccctc taaagcccgc
     7861 aacgtttcaa gtctcgccct gaaacttttg gacgagatta acgaagtttg gctctttgac
     7921 tgtggagaag gtacgcaaaa tcgcattctg gaaaccacaa ttcgaccacg taaggtcagc
     7981 aaaatcttta ttacccatct gcatggagac cacatttttg gtttgccagg tttcctttct
     8041 agccgtgcct ttcaggccaa tgaagagcag acagatttgg aaatctacgg acctcaagga
     8101 atcaagtcat ttgtcttaac tagccttcgt gtgtcaggtt ctcgtctgcc ctaccgcatt
     8161 catttccatg agtttgacca agattctctg ggtaaaattc ttgaaaccga taaattcact
     8221 gtgtatgcag aggagctgga ccacactatt ttctgtgttg gctatcgtgt catgcaaaag
     8281 gatctagaag ggacgctgga tgctgaaaaa ctcaaggctg ctggtgttcc gttcggcccg
     8341 ctttttggta aaatcaaaaa cggccaggat cttgttttgg aagacggaac tgaaatcaag
     8401 gcagcagact atatctcagc gccacgtcca ggtaagatta tcactatttt aggagacact
     8461 cgaaaaacgg atgccagtgt gcgtctggct gtcaatgcag atgtcctagt tcatgagtcc
     8521 acttatggca agggtgatga aaaaattgct cgtaaccatg gtcactcaac taatatgcaa
     8581 gctgcacaag tagcggtaga agcaggtgcc aaacgcctcc tactcaacca tatcagtgcc
     8641 cgtttcctct caaaagatat tagcaaactc aagaaagacg ctgccacaat ttttgaaaat
     8701 gtccatgtgg tcaaagactt ggaagaagtg gaaatctagc agtcacagaa aggataagta
     8761 tgcctactat tctcattacc ggagctagcg gtggtctagc tcaagaaatg gtcaaactcc
     8821 tgcccaatga ccaactcatc ttgcttggta gaaataagga aaaattagcc caactctacg
     8881 gaaattattc ccatgcagaa ttgattgaaa ttgatattac cgacgattca gccctagaag
     8941 ctctggtaac tgatctttat ctccgctatg gcaagattga tgtcttgatt aacaacgctg
     9001 gttacgggat ttttgaggga tttgaccaga ttgctgataa agatattcac cagatgtttg
     9061 aggtcaatac ctttgccctg atgaatctgt ctcgtcacct tgcggctcgt atgaaggaaa
     9121 gcagcaaagg gcatatcatc aacatcgtca gcatggcagg tctaatagct actggcaagt
     9181 ctagtcttta ctcagcgacc aagtttgcgg ctattggttt ttcaaatgct ctgcgactcg
     9241 aacttatgcc ctatggagtc tatgtgacaa cagtcaatcc aggtccaatc cgaacaggat
     9301 tttttgacca agctgaccca gatggaactt atcttaaatc ggttgaccgc ttcctcttag
     9361 aggcagatgc agtggctaaa aagattgtca agattatagg caaaaataaa cgagaactca
     9421 atctcccgat tttgttgaac ctagcccata agttttatac tctctttccc aagctagctg
     9481 ataagttggc aggggaaact tttaattata agtaaaaaga accaatgtgc aggttgttgc
     9541 tagcctacat attggttctt atcatttctc aactatcaaa ctgaacttct tctagtagat
     9601 agtctataaa gcgttcgccc atcttagata ggctggtttt ctcatgctgg atatagacca
     9661 gctcaatcgg gtcatcaata tccagtggga tagaaacgat attgtctccg tttaggttgc
     9721 tgttcaaaat ccctgtcgca atggtataac catccaaacc aatcaagaga ttaaagaggg
     9781 tggcacggtc actgaccaca atggatttct tgtggtgttc ttgagaaaga atctcttctg
     9841 aaaagtagaa ggagttgtgc gtcccttggt catagctgag gtaagggaaa ttctccaaat
     9901 cagacagttt caccttgtct ttctttgcca gagggttggt cttgctgaca aagatatgcg
     9961 gttgcgctgt gaagagatgg tgggctagca ggtgattgtc atccagcatc ttggttaaaa
    10021 catcacggtt gtaactgttt aagaagagga ccccgacctc actgcggaag ttcttgacgt
    10081 cgtcgataat ctcccaagtc cgagtttcac gaaggaagag ttcgtatttc tccatatcgc
    10141 ttttcttgag caaagagaca aaggcattga ccacaaaggc atagtgttga gacgaaacgc
    10201 taaatagttc gcggtgggcg acaggatttt tatagcgttc ctccagaagc tgggtctgct
    10261 cgacaacctg acgggcataa gagagaaact ccatcccatc acgggtcaag gtgattccct
    10321 tgggattgcg gataaagatc tcaatgccca tttcattttc caaatctcgc actgcattgg
    10381 agagacttgg ctgagtgata aacagttgct tggctgcctc attcatggag ccagtttcga
    10441 cgattttgat aatataatgt aattgttgaa ttctcatgtt tttattgtac catacttgcc
    10501 ttagaatcgc taaagaagat aggaaaataa ggagcattgt gtgagtctct tcttgaaaaa
    10561 aacacaaaat tttgctagaa tgagtcttat gaaaacattc tatgatgtgc agcaattcct
    10621 caaacgattt ggtattattg tttacatggg aaaacgctta tatgatattg aactgatgaa
    10681 gttggaacta tctcggattt acgatgcagg gttgatggat aaattagact atctagaggc
    10741 agaagcagtt cttcgcagag agcacaaggt agaattggat tatattgaga aaaatggaga
    10801 aaagaactaa tggttacttg gattttgtgg gcacttatac tagcaatgtt ggcgtggatg
    10861 ggctttaact atcttcgtat tcgccgtgcg gctaaaattg tggacaatga ggagtttgaa
    10921 gccttgattc gtacgggtca attgattgat ttgcgcgacc cagcagaatt ccacagaaaa
    10981 catatccttg gtgcacgcaa tattccttca agtcagttga aaactagtct tgcagccctt
    11041 cgtaaagata aacctgtcct tctctacgaa aaccaacgtg cgcaacgagt tacaaatgca
    11101 gctctttact tgaaaaaaca aggtttttct gagatttata tcctttctta tggcttggat
    11161 tcttggaaag ggaaagtgaa gactagctaa ccttatttcc aaattagtcg aatgatagga
    11221 tgaagctatt gttttatagt ataattaatt tgtataaaat ttaaagagtt cttagaacat
    11281 gaaagaaaag aagaaaaatc cactttttgt gggtatcttg tcgattattc ttggtttgtt
    11341 atttccaatt gtaggcctga ttttgggtat cataggattg gtcttggcta tttcatacca
    11401 aaaagagtca caattagact ataaaataga aaagattctt aatatcttag gaattgtgat
    11461 ttctgtagtt aactggattg tagcaatcgc cttaattttt cgataaataa aagaaaaaag
    11521 ctttagtttt aaagcttttt ttgttttatc gactcatctc taaatagttt ttaatgattt
    11581 gtaactcttt ttggttcaaa gggcggtagt taccttctgc taaatctggg ttcaatgtga
    11641 agtccccaaa ttggattctc ttgaggctag tcaccttaac accaaccgag aggaacattt
    11701 tcttgatttg atgaaatttt ccttctgaaa tggtgataga ggcttgactg atggagggac
    11761 ttgcagatag aatctctagt tttgcgggtt tacagacagt accatctaaa aagacaattc
    11821 ccttttgaaa ggcttggata tggtcaggtg ttagaagtcc attaacctca acttgataag
    11881 tcttatcgac atgatattgg ggatggagga gttggaagcc caagggaccg ttatcggtca
    11941 agagaaggag tcctgttgta tctcggtcca gtcggccgac ggcatagagc ttgtcagact
    12001 ggatgtcaga tggaagcagg tccatgacgg tcggaagttc cttgtctttg ttggctgtaa
    12061 cgacaccagc aggtttatga agcataagat aggtgtgttc atagccttga atgattcgat
    12121 cctgaaaaag gagttcttgt agtcctgtat cgatattttg agctagggag cgggctgggc
    12181 aaccatcgac tagaatttcc cctctgagga gtgcttgctt catggccttt cggctgattt
    12241 tttcttgggc taataaatta tctaaacgca tactgtgcct atcttatcat aagtcgctcg
    12301 ctttgaaaag gcttgacgtg aaaagaaaag cagagtgaaa acatttacac ttgcttgagt
    12361 tatgttattt acttgaaatt atggtataat cgttcagtta gaaaataaat tttgaatatt
    12421 atagaggaaa tcatgacaaa attaagagaa gatatccgta acattgcgat tatcgcccac
    12481 gttgaccacg gtaaaacaac cctggttgac gaattattga aacaatcaga aacgcttgat
    12541 gcacgtactg aattggcaga gcgtgctatg gactcaaacg atatcgaaaa agagcgtggg
    12601 attaccatcc ttgctaaaaa tactgccgtt gcttacaacg gaactcgtat caacattatg
    12661 gacacaccag gacacgcgga ctttggtgga gaagttgagc gtatcatgaa aatggttgac
    12721 ggtgttgtct tggtcgtaga tgcctatgaa ggaaccatgc cacaaactcg tttcgtattg
    12781 aaaaaagcct tggaacaaga ccttgtccca atcgtggttg ttaacaaaat cgataagcca
    12841 tcagctcgtc cagtagaagt agtggatgaa gtcttggaac ttttcatcga gcttggtgca
    12901 gatgacgacc agcttgattt cccagtggtt tatgcttcag cgatcaacgg aacttcttca
    12961 ttgtcagatg atccagctga ccaagaagcg actatggcac caatctttga cacgattatc
    13021 gaccatatcc cagctccagt agataactca gatgagcctt tgcagttcca agtgtcgctt
    13081 ttggactaca atgacttcgt tggacgtatc ggtatcggtc gtgtcttccg tggtacagtt
    13141 aaggttgggg accaagttac cctttctaaa cttgacggta caactaaaaa cttccgtgtt
    13201 acaaaactct tcggtttctt tggtttggaa cgtcgtgaaa tccaagaagc caaagcgggt
    13261 gacttgattg ccgtttcagg tatggaagac atctttgtcg gtgaaaccat cactccgaca
    13321 gatgcagtag aagctcttcc aatcctacac atcgatgagc caactcttca aatgactttc
    13381 ttggtcaaca actcaccatt tgctggtaaa gaaggtaaat gggtaacttc tcgtaaggtg
    13441 gaagaacgct tgcaggcaga attgcaaaca gacgtttccc ttcgtgttga cccaactgat
    13501 tcaccagata aatggactgt ttcaggacgt ggagaattgc acttgtcaat ccttatcgaa
    13561 acaatgcgtc gtgagggcta tgaacttcaa gtatctcgtc cagaagttat cgtaaaagaa
    13621 atcgacggtg ttaaatgtga accatttgaa cgtgtacaaa tcgacactcc agaagaatac
    13681 caagggtctg ttatccaaag cctttctgaa cgtaaaggtg aaatgttgga tatgatttca
    13741 actggtaatg gtcaaactcg tttggtcttc cttgttccag cgcgtggttt gattggatac
    13801 tcaactgagt tcttgtcaat gactcgtggt tacggtatca tgaaccatac cttcgaccaa
    13861 tacttgccat tgattccagg ggaaattggt ggacgtcacc gtggtgccct tgtttctatc
    13921 gatgctggta aggctacaac ttactcaatc atgtctatcg aagaacgtgg tacgatcttt
    13981 gtcaacccag gtactgaggt ttatgaagga atgatcatcg gtgaaaactc tcgtgaaaat
    14041 gacttgacag ttaacatcac gaaggcaaaa caaatgacca acgttcgttc agctactaag
    14101 gaccaaacag ctgttatcaa gacgcctcgt atcttgacac ttgaagagtc tcttgagttc
    14161 ttgaacgacg atgagtacat ggaagtaacg cctgagtcta tccgtttgcg taaacaaatc
    14221 cttaacaaag cagagcgtga gaaagctaac aagaagaaaa aatcagctga ataagagcta
    14281 gaaagagaga aagatggtct atttagtcct aggaatttta ctgctcctac tctatgtatt
    14341 tgcgacacca gaaagcatta aagggacagt caatatcgtc gctatggtat gtattttagt
    14401 ggcactcttg attttattgg ttctatcttt tctgaaaatt tttcaattac caacagaaat
    14461 attcctagca atagccatgt tgatcctagc ttactttagt gtgagagaca tcacactcat
    14521 gccagtcaaa aaaagtaaaa gaagataaaa aagagagttt tggctctctt ttttgctagg
    14581 aattttacaa gattgcaatt tatttttgag ttatcaccat ctcttttgtt tggataatat
    14641 gaatggga
//
LOCUS       DAVGJL010000045        14613 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.045, whole genome shotgun sequence.
ACCESSION   DAVGJL010000045 DAVGJL010000000
VERSION     DAVGJL010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14613
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.045"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..1706
                     /locus_tag="VVE42_001081"
     CDS             <1..1706
                     /locus_tag="VVE42_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEU3073285.1"
                     /translation="NYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGTAGMRG
                     LVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFESAAVL
                     AKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDGGQMPP
                     HDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDVEYLKEVKDVNINPALI
                     EEFGKDMKIVYTPLHGTGEMLARRALAQAGFDSVQVVEAQATADPDFSTVTSPNPESQ
                     AAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAKYILEA
                     HKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEEKHNHT
                     YMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKEYGYYA
                     EKTISVTLSGVDGAEQIKAIMAKFRNNAPTEWNATAITVVEDFKAQTATVADGTVTNL
                     TTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKITNIEAEINAFV
                     K"
     gene            1817..2164
                     /locus_tag="VVE42_001082"
     CDS             1817..2164
                     /locus_tag="VVE42_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU3073286.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     AGTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFIHITDGQINVRCDS
                     SMSAQEIKDFAGL"
     gene            complement(2443..3279)
                     /locus_tag="VVE42_001083"
     CDS             complement(2443..3279)
                     /locus_tag="VVE42_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU3073287.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPEILKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGSRKEDTTLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
     gene            complement(3292..3921)
                     /locus_tag="VVE42_001084"
     CDS             complement(3292..3921)
                     /locus_tag="VVE42_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU3073288.1"
                     /translation="MLELRNINKVFGDKQILSDFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSVLENLTLSPVKT
                     MGMKQEEAEKKASGLLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            complement(3931..4572)
                     /locus_tag="VVE42_001085"
     CDS             complement(3931..4572)
                     /locus_tag="VVE42_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU3073289.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGIVTIISKKVEKKYSYYR"
     gene            complement(4718..5947)
                     /locus_tag="VVE42_001086"
     CDS             complement(4718..5947)
                     /locus_tag="VVE42_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU3073290.1"
                     /translation="MNNSQQMLQALEEQDLTKAEHYFAKALENDSSDLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLVL
                     KADLYQLEGLTDVAREKLLEALTYSEDSLLILGLAELDSELENYQAAIQAYAQLDNRS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELASLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALRIAKQGLEKNPFETRLLLAASQ
                     FSYELHDASGAENYLLTAKEDAEDTEEILLRLATIYLEQERYEDILDLQSEEPENLLT
                     KWMIARSYQEMDDLDTAYEHYQELTGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
     gene            complement(5937..7103)
                     /locus_tag="VVE42_001087"
     CDS             complement(5937..7103)
                     /locus_tag="VVE42_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU3073291.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFLAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFIPRKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVMFIIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVVSGLYELEGEEVKSEQ"
     gene            7247..8260
                     /locus_tag="VVE42_001088"
     CDS             7247..8260
                     /locus_tag="VVE42_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEU3073292.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEEGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNSTIEVLIYDGVGE
                     FVRMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIVVHQDSDNATVFKRNCDNGRLAELSNDFHVPEAVCIR
                     FAP"
     gene            complement(8305..8724)
                     /locus_tag="VVE42_001089"
     CDS             complement(8305..8724)
                     /locus_tag="VVE42_001089"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEU3073293.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDDKDHVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AEHAIEEAQDKHLIDQERRAKIALQRAINRINVGNRL"
     gene            complement(8735..10141)
                     /gene="atpD"
                     /locus_tag="VVE42_001090"
     CDS             complement(8735..10141)
                     /gene="atpD"
                     /locus_tag="VVE42_001090"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEU3073294.1"
                     /translation="MSSGKIAQVIGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMGF"
     gene            complement(10227..11105)
                     /locus_tag="VVE42_001091"
     CDS             complement(10227..11105)
                     /locus_tag="VVE42_001091"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEU3073295.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLADQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            complement(11121..12626)
                     /locus_tag="VVE42_001092"
     CDS             complement(11121..12626)
                     /locus_tag="VVE42_001092"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEU3073296.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYTGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALTHGFLDTVPV
                     DDIVRFEEEFHAFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            complement(12641..13177)
                     /locus_tag="VVE42_001093"
     CDS             complement(12641..13177)
                     /locus_tag="VVE42_001093"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEU3073297.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            complement(13177..13671)
                     /gene="atpF"
                     /locus_tag="VVE42_001094"
     CDS             complement(13177..13671)
                     /gene="atpF"
                     /locus_tag="VVE42_001094"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEU3073298.1"
                     /translation="MHVTVGELIGNFVLITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            complement(13685..14401)
                     /gene="atpB"
                     /locus_tag="VVE42_001095"
     CDS             complement(13685..14401)
                     /gene="atpB"
                     /locus_tag="VVE42_001095"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEU3073299.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNVLEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKIQTI
                     DGTNWWSSPTANLQYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQAIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            complement(14436..14600)
                     /locus_tag="VVE42_001096"
     CDS             complement(14436..14600)
                     /locus_tag="VVE42_001096"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEU3073300.1"
                     /translation="MGVSVGEGLLMNGLFKSVARQPDMLSEFRSLMFLGVAFIEGTFF
                     VTLVFSFIIK"
BASE COUNT         4477 a         3253 c         2670 g         4213 t
ORIGIN      
        1 aaaattacca gaaatgggtt gattttgtgg agcttcctga ctaccttcgt caagatttgg
       61 aaaatatgga cgaaaaaact aaggaagatg ccttctatac aaatcttgaa tttggtactg
      121 caggtatgcg tggcttggtt ggtgctggta caaaccgtat caacatctac gttgttcgcc
      181 aagctactga aggattggct cgtttgattg agtcaaaagg tggaaacgag aaagaacgcg
      241 gtgtagcaat tgcctacgat agccgtcact tctcacctga gtttgccttt gaatctgcgg
      301 cagttcttgc taaacacggc atcaaatctt acgtatttga aagccttcgt ccgactccag
      361 aactatcatt tgcagttcgt catctcaact gtttcgcagg tatcatggtc acagccagcc
      421 acaaccctgc tccatttaac ggttacaagg tttacggtga agacggtgga caaatgcctc
      481 cacacgatgc ggacgctttg actacttata tccgtgcaat cgaaaaccca tttgcagttg
      541 aagttgctga tgtggaaact gaaaaagctt ctggcttgat tgaagttatc ggcgaagctg
      601 ttgacgtaga ataccttaaa gaggttaagg acgtaaacat caacccagcc ttgattgaag
      661 aatttggtaa agacatgaag attgtctaca caccacttca tggtactggt gaaatgttgg
      721 ctcgtcgtgc tcttgcccaa gcaggatttg actctgttca agttgttgaa gcgcaagcaa
      781 ctgctgaccc agacttctca actgtaacat ctccaaaccc agaaagccaa gcagcctttg
      841 cccttgctga agaacttggt cgtcaagttg gtgcagatgt tcttgtcgca actgacccag
      901 acgctgaccg tgttggtgtt gaagttcttc aaaaagatgg tagctacctc aacctttcag
      961 gtaaccaaat cggtgctatc atggctaaat acatcttgga agctcacaaa aacgctggaa
     1021 ctcttcctga aaatgccgct ctctgcaaat ctatcgtttc aactgacttg gtaacgaaga
     1081 ttgctgaaag ctacggcgca actatgttca acgtcttgac aggtttcaaa tttatcgctg
     1141 aaaaaatcca agaattcgaa gaaaaacaca accacactta catgatggga tttgaagaaa
     1201 gcttcggtta cttgattaaa ccatttgtac gtgataaaga tgccatccaa gccgttcttg
     1261 tcgttgctga acttgctgcc tactaccgtt ctcgtggttt gacacttgct gacggtatcg
     1321 aagaaatcta taaagaatat ggctactacg cagaaaaaac aatctctgtt actctttcag
     1381 gtgtcgatgg tgctgaacaa atcaaagcga ttatggctaa attccgcaac aatgctccaa
     1441 cagaatggaa cgcaacagct atcactgtcg tagaagactt caaggcacaa actgctactg
     1501 ttgctgacgg tactgttaca aacttgacaa ctcctccaag tgatgtgttg aaatacacac
     1561 ttgctgacgg ttcatggatt gccgttcgcc cttcaggtac agaaccaaaa atcaagttct
     1621 acattgcagt tgtaggggaa accaacgaag aatcacaagc taagattact aacatcgaag
     1681 cagaaatcaa tgcatttgta aaataaagac ctataaaaga tgagaccaac caacctcatc
     1741 ttttttcgta tcaaatctaa gcaattttag aaactttatg gcatactaga cttatcaatg
     1801 aaagcgaggt aatctcatgg aacaattttt agataacatc aaagaccttg aagtcactac
     1861 agttgtgcgt gcgcaagaag ctcttgataa aaaagaaact gcaaccttct ttatcggtcg
     1921 caaaacttgc ccttactgcc gtaaatttgc aggtacattg tcaggtgtcg tagctgaaac
     1981 caaagctcac atttacttca tcaatagtga agaaccaagc caactcaatg atttgcaagc
     2041 attccgctca cgttatggaa tcccaactgt accaggtttt attcatatta cagatggaca
     2101 aatcaacgtc cgttgcgact cttcaatgtc agcacaagaa atcaaagatt tcgcaggatt
     2161 gtaaaaaatc agctaacaag tcttaaaatc acaaaaacgc ataatgtaag gtgcaaaaac
     2221 cttgatatta tgcgttttat tgttcctaaa acagttacat cagcatttca ttacagaaca
     2281 ttttaatttt ggaagtcaaa tcaattttta acaatgtttt agaaatcaca gtgggctatt
     2341 ccatattcaa ttatccagat tcaatcgcta ttatcgtttt cctagattac aaaaaaaact
     2401 acgttttgct aatcaaaacg tagtctcagc cactatttta tcttactgtc cttcttttac
     2461 ttctttggtt gctacatctt ctccaaacca tttttggctg atttcttgga acttgccgtc
     2521 cttgtaaaga ctagaaaaag cttcatttat cttcttaacc aaggttgtat cttccttacg
     2581 ggatccaacc gcaaaagctt ctgtttctag tccaactgta aagacattat aatcgtttaa
     2641 aacaccttct gcttctaaat aatagtttgc atagacacgg tcaatcaata gaccatcaat
     2701 tcgatcgttt ttcaaatcaa tcaaggcttc attaaaggtt tggtattgat tcgcttcctt
     2761 attagcgaca atattcttca aaatttctgg atttgcttca aagtccgcat aaccagatga
     2821 accagcttga gctcctaatg tctttccagt catatccttt gcagtcgtga taccagatga
     2881 tttcttcgta accaatacct gctcattctt catatatgag ttactgaaag ccaccttttc
     2941 acggcgttcg tctgtagcgg aatagccatt ccaaatcaga tcaatcgttc cttttgtcaa
     3001 ttcagcttct ttcaaatccc aatcaatcgg ttgccaattt accgtgattc cgtatttttc
     3061 aaaaacagct gtagctaaat caatatcaaa tcctgcataa gaaccatctt tctgagcaaa
     3121 tcccattgga acaaaagtac tatcaaatcc aatagtaata gacttgttag actggtactt
     3181 tgaccaatta tctccactag tttcgctaga atttttccca caagctacta agaacaaagc
     3241 agtcatcaga ctgactaata caagcatcca ttttttcatc catttttcct cctatttagg
     3301 ttctactttc aataatacat ctgcgatatt ttcagcaaac tgcaaatcat gggtaaccac
     3361 aatctgggtc atcccaagtt ccctattttg caagattagc ttctccactt ccaaacgtaa
     3421 ttctggatcc agggcagaag ttggttcatc gtagccaatg atttctgggt caatcatcat
     3481 agcacgcgcc aaagccaccc gctgcttttg cccaccagat agtgagaaag gataggcctc
     3541 tgcgtgtcct cctagtccta actgttccaa gagcccactc gccttcttct cagcctcttc
     3601 ctgcttcatt cccatggtct tcacaggcga taaagtcaaa ttttccagaa ctgatagatg
     3661 aggaaatagt tgaaaatctt ggaagacaaa tcccagtagg ttgcgcttct gcaattcatc
     3721 cagctctaaa ggttgtccat tataaaagat ttgccctgaa tcaatggttt caagacctgc
     3781 aagcatacgt aaaagagttg tcttacctcc accagaaggt ccaacgatag ccaggatttg
     3841 cttttcagga atacttagac tgaaatcaga caggatttgt ttgtctccaa agactttatt
     3901 gatatttcgt aattctaaca tagctgcccc ctatctataa taactatact tcttctcaac
     3961 ttttttggaa ataattgtca caatcccaat taaaatcaag taaatggctc ctgccaagaa
     4021 cataggaacc agactagcat cgcggttagc agctgttcga ctagccaaga taaggtctga
     4081 aattccgaga gcatagacca aagaagtatc cttgaccaaa ctcataactt cattaaagac
     4141 actaggaaga acgatcttgg tcacttgggg caagataata tagcgcactc tgtcaaaagg
     4201 gctaaacttc aagaccttgg cagcctcata ctgtcctctt ggaatagtgt caattccccc
     4261 acggaaaatt tctgcaaagt aagctgcata gttgagaaca aaggcaataa tagctgcagg
     4321 aaggcggtct aaacgaatcc caatacttgg gagcacataa tagataaaaa tcagttgcaa
     4381 gagtaagggg gttcctcgca taacccagat gtaaatgtta atcagataat ggaggggctt
     4441 ccaatggact tgcaaggcaa aggcaatcaa aacgcccaac ggaatcgaaa atagcaagac
     4501 cagtgcaaag acctgtacag tcgtgctcgc accattcagt aaactcggta atatctcaaa
     4561 taaataagac atactgcacc tcctaaaagt aattgttcct attatagcat gaataaacaa
     4621 gattaccaag tatttttgta aaaagattct accatataca aataaaaacc gtagctatca
     4681 aaagagataa actacagttc tatatgttta aacattctta caatctctca aacagttctt
     4741 gcatttgcac atcatctgga accagtttta agtaagtgtg agcatggact tttgcttctt
     4801 caaaatgtcc caattcacgc aagagataga tatagtgttc cagaaattct ggattgtcct
     4861 tcaaatctcc tgtcaactct tgataatgct cataagcagt atccaaatcg tccatttctt
     4921 gataagaacg agcaatcatc cacttggtca aaagattttc tggctcctca ctctgcaagt
     4981 ctagaatatc ctcataacgc tcctgctcca gataaatagt ggctaaacga agcaagattt
     5041 cttctgtatc ctcagcgtct tcttttgcag taaggagata attttctgca ccactagcat
     5101 catgcaattc ataagaaaat tgtgaagcag ctagcaagag gcgagtttca aagggatttt
     5161 tctctaatcc ttgcttagcg atacgcaggg cttcttgaac ttgatgttcc ttatgtaaag
     5221 cctgactgta cccatactca tagccttcaa agtcaggaga aatggtatca agctgcttaa
     5281 agtagagggt ggctttttga tattcttctt gatcaaaata aagactggcc aactcaaaag
     5341 ctgttaagtc atcgtattct aactccaggg ctttttctaa aaactcagta gccgtttcaa
     5401 atttccctaa ctgagcatag gcaaagccaa ttcgttgata ggtggaaatg cccgtttgct
     5461 cataaatcga gcgattatct aactgggcat aggcttgaat agccgcttgg taattttcca
     5521 actcactatc caactctgcc aaacccaata tcaagagaga atcctctgag taggtcaagg
     5581 cctccaataa tttctcacgt gccacatctg tcaaaccttc cagctggtaa aggtctgcct
     5641 tcaaaaccaa agacgagaca taccagtcac tgtcagcttg gatttcctca agataggtaa
     5701 aggcttcttc tatttgacca tcctcgctag caattgcagc tagattaaga tgaacctctg
     5761 gaaaatcctc tacaattttc aggtaaattt ccttggcctg aggatagaaa ccaatccctt
     5821 caagataagt tgccaactca tacagaagat cacttgaatc attttctaaa gctttggcga
     5881 aataatgctc agccttagtt aaatcttgct cctccaaagc ctgtaacatc tgttgactat
     5941 tgttcactct tgacttcctc accctctaat tcatatagac cactgactac cttataccat
     6001 tcgaaaatgg ctgaaatgac aaccttagca gaggcataaa ctggaatacc aagtaaaact
     6061 ccccagatac caaacataga tcctgaagtt aacaaaacaa agagaacatt aatagggtgg
     6121 atgtttaatt gacttcccaa aatcaatgga gagacaaaac ggccttcaat agtttgttct
     6181 acaataaaga caatcactac tttcaaaagc atgactggac cagcaatcaa acccaatact
     6241 agggcaggaa gcatggctag aaaactacca agataaggga ccagatttaa aataccagca
     6301 gtaaccccca gcgtaaccgc atagcgtaga ccaataatct tgaagaagat gataaacatt
     6361 actgctacaa taatagccac tgtcacttgc cctcgaacat agttggacaa ctgttgattc
     6421 acatctgata gaacttgtcc aacaggttcc ttcaattttc ttggaatgaa ttgggtcaaa
     6481 tagttacgca agcctttccc atcacgcaag agataaaaga gcatgaaagg aacgataatc
     6541 aaggcaacaa tcacttgaga agccccgcta ataaaggcac tcacccagtt gactgcctga
     6601 gatgaaacct tacttgccaa aactgtagcc tggctagaaa aattggtcaa aacttgctct
     6661 aattgaggtc tgaaatcatc tggcagatgc tgggctacca atccattaac aatcctatct
     6721 atatcttcta agtaaacagg aacgtttctt gcaaaggtca aaacctgacg ttgcagattt
     6781 ggaatggcga ctgccaagcc ccaaatgata aagagagcga tgataacaaa gacaatagtg
     6841 atagctataa cacgattaac cttatgcttc tccatccaat caacaatagg attcaacaaa
     6901 taatataaca aaccagacaa aatgactggc aacatcacaa ctgctaaaaa gtctaaaaca
     6961 ggtgaaaata gaaaactaat cttacttaaa ataaaaagat tcaatcccaa taataaggtt
     7021 actaaaaata ccgtaattgc cttgttatct aaaaaccact tgaaaaacca agataggcta
     7081 aaatgtttct ctttttgctc cataaatact tcctttctat tgttctctca ttataccatt
     7141 tttgaaacaa aataactctt ttttaaagtg cttacatcta attggatgtc tttaatttgc
     7201 ttgtttttgt gatataatag tcttatcttt acatagaggt atagtaatga aagaaactgt
     7261 ttattttgga acttatacac gtcgtacttc tcaagggatt tacaaggcag actttgatac
     7321 agaaactggt cagctttcaa atctagaact ttttgcagct gagccaagtc caacctacct
     7381 tgcctttgac cagcaccaac atttatacac tgttggtagc caagacgata aggggggaat
     7441 tgcagcctat caaactgacg ggactgtgtt aaatcatgtt gttgaagaag gagctcccca
     7501 ctgttatgtt gctgtcgatg aaaagcgtga tttggtttac gcagccaact atcacaaggg
     7561 acaagtcctt gtttataaac gccaggaaga tggtagtctt ctacttagtg atatggatca
     7621 acacagtggc caaggtccac atgaaaatca agcttccccc catgttcact atacagattt
     7681 aacacctgac cactatctag tgacctgcga cttgggaact gaccaagtca tcacctatga
     7741 cctcgatcaa gaaggaaaat tatctaagct ctatacctat cacagcaagc caggagcagg
     7801 ctcacgccat atcattttcc ataaccacta taaaatcgct tatctcattt gtgaactcaa
     7861 tagtactatc gaagttttaa tctacgatgg cgttggcgaa tttgtacgta tgcaggttat
     7921 ttcaacttta ccagaagctt acgaaggctt taatggaacc gctgctattc atctctctaa
     7981 agacggtaaa tacctctacg cttctaaccg tggccatgat tctatcgcag tatataccat
     8041 ccttgcggac ggtagcttag agttattaga aatcgttcca acgcatggtc agactccacg
     8101 tgattttgat ttgacacccg accaaaaatt tctcattgtt gtccatcaag actctgacaa
     8161 tgcaactgtc tttaaacgta attgtgacaa tggtcgtcta gcagaactct ccaacgactt
     8221 ccatgttccc gaagcagtct gcatccgttt tgctccttaa aacaccataa aaaatgaact
     8281 tggtattttc aagttcattt tttcttatag tctatttccg acattaatac ggttaatggc
     8341 acgttgcaaa gcaatcttag cacgacgttc ttggtcaatc aaatgtttgt cttgtgcttc
     8401 ttcaattgca tgttctgcac gaagtttggc acgttctgca cgactgatat cgatatcacg
     8461 agcacgttct gcagagtcag cgacgattgt aatcatatca ttggcaattt caataacgcc
     8521 accgtttact gcaatccagt tcacgtgatc tttatcatcg atacgtttta cctttacttc
     8581 atcaactgct aaaaccgcaa tcatattttc atgtcgtggc aagatcccca tctcaccatc
     8641 cagagttcga accgatacat agctggcatg gtgatcatag acgagaccat ctggtgtcac
     8701 gatctggaca gttaactgag ccataggtca cctcttaaaa tcccattttt tcagcttttg
     8761 caatcacatc ttcgatagaa cctacaccac ggaaggcatc ttctggcaag tggtcgtatt
     8821 taccatcaag gatttcctta aagccacgta cagtttcagc aactggaaca taagaacctg
     8881 gctgaccagt aaattgttcc gcaacgttga agttttgtga caagaagaac tggatacgac
     8941 gggcgcgagc aaccaaggtc ttttcttcat cagaaagctc atccatacca aggatagcaa
     9001 tgatatcttg caattcatgg taacgttgaa ggacacgttt tacttcagca gcaactgcat
     9061 agtgctcttc tccaacgatt tcaggtgcca aggcacgtga gcttgaagca agtgggtcaa
     9121 cggctgggta gatacccaat tgtaccaatt tacgttccaa gtttgttgtt gaatccaagt
     9181 gagcgaaggc tgttgctggc gctgggtcag tatagtcatc cgctggcaca tagatagcct
     9241 ggatagaggt tacagaaccc ttcttggttg atgtgatacg ttcttgcaat tgacccattt
     9301 ccgtagcaag tgttggttgg taaccaacgg ctgatggcat acgacccaaa agggcagata
     9361 cttctgaacc agcctgagtg aaacggaaga tattatcgat aaagagaagc acgtcttggc
     9421 cttccacatc acggaagtat tcagcgattg tcaaaccagt aagggcaaca cgcatacgtg
     9481 ctcctggtgg ctcattcatc tgaccaaaga ccatggctgt tttctcgata acgcctgatt
     9541 ctttcatttc ccagtaaagg tcattcccct cacgagtacg ttccccaaca ccagtaaata
     9601 ctgaaatacc accgtgctct tgggcaatgt tgtgaatcaa ttcttggatt aagacggttt
     9661 taccaactcc ggcaccaccg aaaagtccaa ctttaccacc tttaaggtaa ggggcaagaa
     9721 ggtcaataac cttgatccct gtttcaagga tttcagaaga ggtagacaac tcatcaaaag
     9781 ttggagcttt tttatgaatt ggctgacgct ctgcgtcttc tgtaaaagga gcttccaaat
     9841 caatggtatc tcccaaaacg ttgaagacac gtcccaaagt ttctttacct actggtacag
     9901 agattggacg acctgtgtcc aatacttcca ttccacgagt caacccatct gttgattcca
     9961 tggcgatagt acgaaccata ccatctccta actccaaggc tacttcaagg acgatttttg
    10021 tttttctttc gtcatttttg tagacgacaa gtgcattgtt aatctcagga agtttttccc
    10081 ctgctgcaaa caaaacgtct acaacgggac cgataacctg agcaatttta cctgaactca
    10141 tctccttctc ctattctata taagatactg tcggttccta gctcaactag gtcctaagtt
    10201 cattttcata cgagctggac tagagcctat tctaaggcac tagcacctgc tacgatttct
    10261 gtaatttctt gtgtaatcgc cgcctgtctg gcacggttat actgaattgt caaatcattg
    10321 atgactttct tagcattatc tgtcgctgtt tgcatggctg tcatacccgc agcattctca
    10381 gctgtcttgg catcgataat ggcaccgtaa atcatacttt ctgcaaactg aggcaacaac
    10441 tgctccagaa tttcttctcg gctggtttcc aattcaaaag tcaagctgta ctcttcatcc
    10501 gcctcatttg gatccaagtc aacaatcgga agcatttgtt ccacacgcat ttgactggtt
    10561 agcgtattga catggtggtt gtagcaaaca taaagctcat caaagagttc attttggtac
    10621 atttcaacag tttttgaaat aatcttacga acttgatcaa agctaggttg gtctgccaag
    10681 ccacgtaatt cataaagtgg ttgaataccg cgagccttaa agaaatcagc tcccatccca
    10741 ccgatacaga tcatttcaaa acctttaccg tctgggtggt attcttcttt caactccata
    10801 acagctttca aaatagagga attataacct ccaaccaaac cgcggtctga agtgataacg
    10861 atatagcctg tcttcttcac agaacggcta atcaacatcg gattagttga agcaccagct
    10921 ccattaccat gaaggatatc tgtcaaaagt ttacgcactt tctgagcgta aacttggaag
    10981 ttgcgagcag cttcttcaga gcgacctaac ttagcagccg ataccatttg catagcatta
    11041 gtgatttgac tcgtattttt tgttgaggcg atttttgttt taatatcatt tagagacact
    11101 gccatctgac acctctattc ttattggaag ctagattgat tgagaaactc tgtaatcgca
    11161 gcatccaaga ctgcttcttc tggcaagtct tttgtatcac gaatggtttc caaaatctct
    11221 ggatgttgag catcaaagaa ggcatggaac tcttcctcga aacgaacaat atcatctact
    11281 ggaacagtat ccaagaaacc atgtgtcaaa gcataaagaa tggttacttg tttctcaaca
    11341 ggtaatggtt tgtgaacagg ttgtttcaag acctcaacgg tacgacgtcc acggttcaac
    11401 ttagcctgtg ttgctgcgtc caagtcagaa ccaaacttag taaaggcttc caactcacgg
    11461 tatgaagcaa ggtcgatacg aagtgtacca gcaaccttct tcatggcttt gatttgtgca
    11521 gaaccaccta cacgagatac agatgaaccc gcatcgatgg ctggacgaat acctgcattg
    11581 aagaggccat cgccaaggaa gatttgtcca tcagtgatag aaatcacgtt ggttgcgata
    11641 taggctgaga tatctcctgc ttgtgtctcg ataaatggta gggctgtaat tgatccacca
    11701 ccaagttcat cagaaacttt agctgagcgc tcaagcaaac ggctgtggag atagaaaaca
    11761 tcccctggga aggcttcacg acctggagga cgacgaagca agagcgatag ttcacgataa
    11821 gctaccgctt gttttgatag atcatcgtat acaatcaaaa catgcttacc ttgatacata
    11881 aattcttccg ccatagcaac cccagtataa ggagctagga agagcaatgg agatggttgt
    11941 gaagcagagg ctgtcacaac gattgtgtag tccaaggcac cgtactgacg aagtgtttct
    12001 acttgcgtac gaactgttga ttctttttgt ccaatcgcga cgtagataca gatcatatct
    12061 tgatcttttt ggttcaagat tgtatcaatc gcaatggttg ttttccctgt ctgacggtca
    12121 ccgataatca actcacgttg accacgacca atcggtacaa gggcgtcaat agctttcaaa
    12181 ccagtttgca atggttctga aacagactta cgttgcataa caccaggagc tggtgcttct
    12241 actggacgag ttttatcagt gtggatttct ccaagaccgt caactggacg accaagcgga
    12301 tccacaacac gaccaatcag actttcacct acagggactt ccatgatttt ccctgtacgg
    12361 cggattgtat cgccttcacg gatatctgta aagtcaccta ggatgataat accaacgtct
    12421 gttgactcca agttttgagc cataccataa gagccgtttt caaaattcaa caactctcca
    12481 ctcatgacat tttcaaggcc atgagcacgc gcgataccgt ccccgatata ggttacaaca
    12541 cctgtttcag tcacatcaaa attgggtttg aaattttcaa tttgttgctt aattaaagcg
    12601 ctgatttctt gtgcgttaat tgccaaaaga acaccacttt ctatttcaaa ttttctttaa
    12661 caactttaag ttgttgttta atactcacat caattgtctt gtgattggca aaaatgacaa
    12721 aaccaccaat gagactttca tcgatttgtt cttttacact ccttactttc agagacattt
    12781 ttttctcaat caaagggagc aaacgagtct tctgttcatc agttagagga tgagcagacg
    12841 taatcgtcac ttcaaatcga tttgtttctt tttcaagtcg gttcaagcaa tctacaagca
    12901 catcataaaa aagatttgct ctgtgattgt aggccagaac ctggataaag ttttgtaata
    12961 aaggcgacac agaatcttgg aaaaaagcaa ttgttttttc cttatccgac tcgtctactg
    13021 ccacttgttt taaaaaagaa ggcagacctg ttttttcaac aacttgcttg atttgagtca
    13081 agtctgaaaa gatacggtct tcttctcctt tttcaagtac caattggaca aaaggcatgc
    13141 tgtatttttc aattaccttt actgttttct tgtccattaa gcttctccta gctgatcgat
    13201 atactgatca atgagtgctt tatgggcatg actgtcaagg ttttgtgaga tgattttacc
    13261 agctaagctg atggtcaaat ctgcgacctc acccttaaca ctctgtaaag cttctacttt
    13321 attttgagca atttcttgat tggctttttc ttttaagtgt cctgcttcta gtttagcatc
    13381 tgctaagata ttagccttac tttgctcagc tgtttccttt gcattttcaa tgattgtctt
    13441 agcttcttta cggctaccag ccaattcatc ttcgcgtttt tgagccaata cttctgcttt
    13501 ttgacgggct tcttcagctc tgtcaatatc tgaagcaatt ttttcagctc tttcttcgaa
    13561 aatgcctgta atattagacc atgcaaattt tttaatcaag actagcaaaa gaataaaaga
    13621 gccagtgatt aaaacaaaat taccaattaa ttcacctact gttacgtgca tcagttactc
    13681 ctttctattc ctcttcaata ttaatcttat tccctaaata cacagatgtc aaaagagtaa
    13741 aaacataagc ttggatgcag gaaataaaga cagaaaatgc agtccaagcc aaattagctc
    13801 caaaggctac tggataccaa taaatagctt ggtgggaaag aagaagtaac aaactcgtca
    13861 tgacctctcc tgcaaagata ttcccaaaaa tccgcaaagc caaagataag aagtttgtaa
    13921 attcttccaa gatattcatc ggtatgacaa aaacaggact cataaaagat tttatacttt
    13981 ttttaaatcc acgacgacga acgctttcta tatgtgtcaa caaaatgaca agaaaagata
    14041 aggttaagtc atactgtaaa ttagcggttg gcgaactcca ccagttagtc ccatcgatcg
    14101 tttgaatctt tgtcattaag ccaaggttat tggcaatcac catgaaaagg aataaacaaa
    14161 ggaaaaagag tgagtaatct ttcatgtagc gcgaaccaat gttaggttct gtaaatccaa
    14221 taacaaagtc atagacatac tcaagtacat tttgctttcc tttgggtttc aaggtcatat
    14281 tgcggcttgc ccaataaata aagacaaaaa taactcccac aatcaacaaa gtcatggcta
    14341 acatagtcag attgaagata acaggaccaa tagagatgat tggattaata ctttcttcca
    14401 tctaaaatcc tcccttttct tctcgttcca tgtatttatt tgataataaa tgagaagaca
    14461 agagttacaa agaaagttcc ttcaataaag gcaacaccta aaaacatcaa actacgaaac
    14521 tcagaaagca tatctggttg gcgtgctact gatttaaaca gtccattcat caataaacct
    14581 tcaccgacag atacgcccat acaggcaata cat
//
LOCUS       DAVGJL010000046        14376 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.046, whole genome shotgun sequence.
ACCESSION   DAVGJL010000046 DAVGJL010000000
VERSION     DAVGJL010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14376
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.046"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            81..878
                     /locus_tag="VVE42_001097"
     CDS             81..878
                     /locus_tag="VVE42_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEU3073301.1"
                     /translation="MISSEFISKIEFACNKKEGLYSQSKFKYAIRSMFAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSFLKKISW
                     RKTAEILLYCTLFNLIGALIAGWGFAHSAAYANLIHDSFISGVVEMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGAKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ADSIANFGVGNMLRHWGVTFIGNFIGGGLLMGLPYAFLNKNEDTYVD"
     gene            1026..1148
                     /locus_tag="VVE42_001098"
     CDS             1026..1148
                     /locus_tag="VVE42_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEU3073302.1"
                     /translation="MTRDFKFETLQLHAGQVVTPATKSRAVPIYQTTSFVFDDT"
     gene            1170..2285
                     /locus_tag="VVE42_001099"
                     /pseudo
     CDS             1170..2285
                     /locus_tag="VVE42_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231001.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
     gene            2381..3655
                     /locus_tag="VVE42_001100"
     CDS             2381..3655
                     /locus_tag="VVE42_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEU3073303.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMRQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHQALLAKDKEV
                     QDLENQLATLRLEHENQLQKTLSDLEKEHDQVKNQLLLQEKENELSLASVKQNYEAQL
                     KAASEQVEFYKNFKAQQSTKAIGESLEQYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRREKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            3665..4543
                     /gene="truB"
                     /locus_tag="VVE42_001101"
     CDS             3665..4543
                     /gene="truB"
                     /locus_tag="VVE42_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEU3073304.1"
                     /translation="MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMVEFMQDEGKIYEGEITLGYSTTTEDASGEVVAETPVLSPLDERLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVTIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALTLEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLTPEDATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
     gene            4784..5086
                     /locus_tag="VVE42_001102"
     CDS             4784..5086
                     /locus_tag="VVE42_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEU3073305.1"
                     /translation="MMRNEFRERVEQLLQQKEINENSELSHLFRLAIQNLDRNEKYQT
                     VMANLSQGLSLYLMTHHYQAPKSVIDFGLWIAKAPSQERGRLAFLQMLAQTLQGFR"
     gene            5194..5832
                     /gene="udk"
                     /locus_tag="VVE42_001103"
     CDS             5194..5832
                     /gene="udk"
                     /locus_tag="VVE42_001103"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEU3073306.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            5960..7300
                     /locus_tag="VVE42_001104"
     CDS             5960..7300
                     /locus_tag="VVE42_001104"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEU3073307.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQVTIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKAEPDG
                     VGALAIQFEQLKKKLTEEGLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEGAAEEIARNIARANQRDDLDLLIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLAHLQNQEK
                     RMATAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     KQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAEAKRLSEALLM
                     LDTSRIVARGYAIVKKEESIVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            7278..7490
                     /locus_tag="VVE42_001105"
     CDS             7278..7490
                     /locus_tag="VVE42_001105"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEU3073308.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            7487..8362
                     /locus_tag="VVE42_001106"
     CDS             7487..8362
                     /locus_tag="VVE42_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEU3073309.1"
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLEALQVAIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDPYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQTIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQLPFE
                     TESIVSVVESLRING"
     gene            8355..9170
                     /locus_tag="VVE42_001107"
     CDS             8355..9170
                     /locus_tag="VVE42_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEU3073310.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMLQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     SLILPALHRVLADQGQVVALVKPQFEAGREQIGKNGIIRDAKIHQNVLESVTAMAVEA
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQILAEIKEAVERAHSQFKNE"
     gene            9163..9594
                     /locus_tag="VVE42_001108"
     CDS             9163..9594
                     /locus_tag="VVE42_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU3073311.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIDTFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            9601..11268
                     /gene="recN"
                     /locus_tag="VVE42_001109"
     CDS             9601..11268
                     /gene="recN"
                     /locus_tag="VVE42_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEU3073312.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRAIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKARIEMLEFQMAEIEAANLQAGEDLALNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSSLSETYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVRFSKGKFSREGNEMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQHGQVLAISHLPQVIAIADYQFFIEKIS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            11270..11998
                     /locus_tag="VVE42_001110"
     CDS             11270..11998
                     /locus_tag="VVE42_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEU3073313.1"
                     /translation="MTDYYVIGDVHGKAGMLEDLLKTWDGQPQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYRRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEASDLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFTDQGMTEHHFIENDGFVAED"
     gene            12077..13900
                     /gene="lepA"
                     /locus_tag="VVE42_001111"
     CDS             12077..13900
                     /gene="lepA"
                     /locus_tag="VVE42_001111"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEU3073314.1"
                     /translation="MNLEELKKRQRKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPERVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNSKTFDVAEVGIFTPKAVGRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
     gene            complement(13981..>14376)
                     /locus_tag="VVE42_001112"
     CDS             complement(13981..>14376)
                     /locus_tag="VVE42_001112"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073315.1"
                     /translation="LAAGYSKHSFEVVDKTDEVSSKHGFEVVDETDEVSSKHGFEVVD
                     ETDEVSSKHSFEVVDKTDEVSSKHGFEVVDETDEVSSKHGFEVVDETDEVSSKHGFEI
                     VDETDEVSNHTYGKATLTWFEEIFEEYKN"
BASE COUNT         4236 a         2651 c         3397 g         4092 t
ORIGIN      
        1 ttttatgaaa tacagaaaac gcttcctaaa acctaaagtt tgtgatataa ttatcaagaa
       61 aaaagattta ggagtttatt atgatttctt cagaatttat ctcaaagatt gaatttgctt
      121 gcaataagaa agaaggtctt tatagtcaaa gcaaatttaa gtatgcgatt cgttcgatgt
      181 tcgcaggtgc atttttaacc ttcagtactg ctgcaggtgc agttggggct gacttgatta
      241 ataaaattgc accaggtagt ggacgcttcc tctttccatt cgtttttgct tggggcttgg
      301 cctacattgt ttttttgaat gccgagttgg tcacttcaaa catgatgttc ttgactgctg
      361 gtagtttctt aaaaaaaatc tcttggagaa aaacagctga gattttacta tactgtacct
      421 tgttcaacct tatcggagcc ttgatagcag ggtggggctt tgctcattcg gcagcctatg
      481 cgaatctgat acacgatagt ttcatctcag gtgttgttga gatgaagtta ggccgctcaa
      541 atgaattggt cttgcttgag gcgattttgg caaatatttt cgtaaatatt gcgattctgt
      601 catttatttt ggtcaaagat ggtggtgcca aactttggct tgtgttgtca gctatttaca
      661 tgtttgtatt cttaacaaac gagcacattg cggcgaactt tgcttctttc gcgattgtga
      721 aattcagtgt tgctgcggat tcaattgcca acttcggtgt tggaaatatg cttcgccact
      781 ggggtgtgac tttcatcgga aactttatcg gaggaggcct cttgatgggt cttccatatg
      841 ccttcctcaa taaaaacgaa gatacttatg tagattaaga aaatgagcac gattgagtcg
      901 tgcttttttc attttcaaaa taagataata gctatttctt atatcaaaat atagaaaact
      961 gatatttgta aactataact caaggtgcta caatatcctt aataaaataa tatggaggtc
     1021 accttatgac tcgtgatttt aaatttgaaa ctctacaact acatgctggt caagttgtga
     1081 ctccagctac taagtctcgt gcagtgccga tttatcaaac aacatctttt gtttttgatg
     1141 acacgtagga aggtgctgat ctgtttgcct tgaggaaacc agggaacatt tatactcgta
     1201 tcaccaatcc tacaacagct gcccttgaag gtggtgttga agcgctagca acagcatcag
     1261 gtatgactgc agtgacttat acgattttgg cgcttgccca tgctggtgac catgtagtgg
     1321 ctgcttcgac tatttacggt ggaaccttca atcttttgaa agaacccctt cctcgttatg
     1381 gtatcacaac aacctttgtc gatattgata atttggagga agtagaagca gctatcaaag
     1441 acaataccaa gcttgtcttg attgaaacct tgggtaaccc cttgattaat attccagacc
     1501 tggaaaaact ggcagagatt gctcataaac atcaaatccc acttgtgtca gacaatactt
     1561 ttgcaacacc ttatttgatt aacgtcttct ctcatggcgt tgacattgcc attcactctg
     1621 cgactaagtt tatcggtggg catggtacaa ctattggagg aataattgtc gatagtggtc
     1681 gttttgactg gacggcttca gggaaattcc cttaatttgt tgacgagggt ccaagctgcc
     1741 acaatttgag ctatactcgt gatgtgggtg cagcagcctt tattatagct gttcgagttc
     1801 aattgcttcg tgatacaggt gcagccttgt caccattcaa tgctttcctc ttgctacaaa
     1861 gacttgaaac cctttcactt cgtgtggaac gccatgtaca aaatgctgag acaattgttg
     1921 attttcttgt caaccatcct aaggtagaaa aggtaaatta tccaaaactt gcagatagtc
     1981 cttatcatgc cttggctgag aaatacttgc caaaaggtgt cggttcaatc tttaccttcc
     2041 acgtcaaagg tgacgaggaa gaagcacgca aggtcattga taatttagaa atcttttctg
     2101 accttgcaaa cgcggcagat gccaagtctc ttgttgttca tccagcaaca accactcatg
     2161 gtcaattgtc agaaaaagac ctagaagcag caggtgtcac accaaatcaa atccacttgt
     2221 ctatcggact tgaaaatgta gatgatttga ttgaagactt gcgcttggcc ttggaaaaaa
     2281 tttaaagaaa aagaagataa acagtggttt tcgactcgct gtttttgatt ttccttcatg
     2341 catgatataa tggttacaga agtctagaaa gaggaatgta atgaacgaaa tcaaatgtcc
     2401 caactgtggg gaagtcttta cagtaaatga aagccagtat gctgaacttt tgtcccaagt
     2461 gagaacggca gagtttgata aggaactaca cgataggatg aggcaggaac tggccttggc
     2521 tgagcaaaag gctatgaatg agcaacagac taaactggct cagaaggatc aagaaattgc
     2581 gcaattacag agtcagatcc aaaactttga tacagaaaaa gaattggcca agaaagaagt
     2641 tgaacagaca agccatcagg ccttattggc taaggacaag gaagtacagg atttggagaa
     2701 ccaattggcc actttgcgtt tggagcatga aaatcaactg caaaagaccc tttctgacct
     2761 agaaaaagaa catgatcagg tcaaaaatca gctcctcttg caagaaaagg aaaatgagtt
     2821 gtctttggct tctgttaagc aaaactatga agcccagctt aaagcagcta gtgaacaagt
     2881 cgagttttat aagaatttta aggctcaaca atctacaaaa gcgattgggg aaagcctaga
     2941 acagtatgca gagagtgagt ttaacaaggt tcgtagtttc gcctttccaa atgcttactt
     3001 tgagaaggat aacaaggtct cttcgcgtgg gtctaaaggg gactttatct tccgtgagtg
     3061 tgatgaaaat ggagttgaaa tcatttctat catgtttgag atgaaaaacg aagcggacgg
     3121 aacagagaag aagcacaaga atgcagattt ttacaaggaa ttggacaagg accgtcggga
     3181 gaagaactgt gagtatgccg ttttggtgac catgcttgag gccgataatg actactttaa
     3241 cacagggatt gttgacgtca gccacgagta tgaaaaaatg tatgttgttc gtcctcaatt
     3301 ctttatccag ttgattggtc tcttacgtaa tgcggcgcta aattccctaa aatacaagca
     3361 ggagttggcc ttggttcgcg agcaaaatat tgacattacg cattttgagg aagatttgga
     3421 tgcctttaag ctagcttttg ctaagaacta taattcagct tcgactaact ttggaaaagc
     3481 tattgatgaa atcgacaagg ccatcaaacg catggaagag gttaagaaat tcctgaccac
     3541 atctgaaaac caactccgtc tagctaacaa caaattggaa gatgtctctg ttaaaaaatt
     3601 gacccggaaa aatccaacaa tgaaagcgaa gttcgaagca ctgaaggggg agtagaaagc
     3661 aaaaatgaac ggtattatta acttaaaaaa ggaagcagga atgacctcgc atgatgcggt
     3721 ttttaaactg cgtaagattt tgggaaccaa gaaaattggt catggtggaa ccttggatcc
     3781 ggatgtggtg ggtgttttgc cgattgcggt tggcaaggcg acacgcatgg tcgagtttat
     3841 gcaggacgag ggtaagatct atgaggggga aatcactctg ggctattcca cgacgactga
     3901 ggatgctagt ggggaagtgg tcgcagaaac ccctgttttg tctcccttgg atgaaaggct
     3961 tgttgatgaa gcgattgcta gcttgactgg gcctattact cagattcccc ctatgtattc
     4021 ggcagttaag gttaatggtc gcaagctcta tgagtatgcg cgtgctggtc aggaagtgga
     4081 gcgtccagaa cgtcaggtga ccatttatca atttgagcga acaagtccga tttcttatga
     4141 tggccaactt gcccgattca cttttcgtgt aaaatgcagt aaagggactt acatccgtac
     4201 tttgtcagtt gatttgggtg aaaagcttgg ttatgcggct catatgtccc atttgactcg
     4261 tactagtgct gctggcttac aattagaaga tgctcttacc ttggaggaaa ttgctgaaaa
     4321 agtagaggct gggcaattag attttctcca tcctttagag attgggacag gtgaccttgt
     4381 caaagttttc ctaactccag aagatgctac agaagttcgc tttggtcgtt ttattgagct
     4441 agaccaaacg gacaaagaac tggctgcctt tgaagatgat aaattgttag ccattctaga
     4501 aaaacggggc aatctctata agccaaggaa ggtttttagc tagatcgttt aggaataaaa
     4561 atcgggtgat agataacaat tgcttgataa aaccccatac taatagtaga atggttttgg
     4621 gaattataat attccaattg ttgcgagttg tagatactca aataatctat atagaaattt
     4681 agaggtgtga aatgaagcaa cttaaaattc tttcagataa atatttagag tccattacag
     4741 gttctggtgg gaacttaggc ccaggatttg gtgtgataat tccatgatgc gaaatgagtt
     4801 tcgagaaagg gtggagcaac ttcttcaaca aaaagaaata aatgaaaata gtgagttgag
     4861 tcacctgttt cgtcttgcta tacaaaattt agacagaaat gaaaaatacc aaacggtcat
     4921 ggccaatttg agtcaagggt tgtcacttta cctcatgacg catcattacc aggcacctaa
     4981 gtctgtcatt gattttggtt tatggattgc caaagctcct agccaggaaa gagggagact
     5041 ggctttcttg cagatgcttg ctcaaaccct acagggcttt cgttaaaact gtcgaacatc
     5101 agagggaaga ctctggtgtt ttttgctgat gagactataa attttgtaaa aaatgtgata
     5161 gaatagacga cggataaaat aacggaggat agcatgcaaa atagaccaat cattattgga
     5221 gtgacaggtg gttctggtgg tggtaaaacc agtgtttcaa gagccatttt atcgcatttt
     5281 cctgatgaaa agatttccat gattgagcat gattcatact acaaggatca gtctcatttg
     5341 acctttgaag agcgtgtcaa aaccaactat gaccatcctt ttgcctttga tacagacttg
     5401 atgatcgagc agattaagga attgttggca gggcgtccgg tggacatccc tacctacgac
     5461 tatacagagc atacacggag tagcaagacc tatcgtcagg agcctcaaga tgtctttatc
     5521 gttgagggca ttttggtctt ggaggacaag cgtctgcgcg atttgatgga tatcaagatt
     5581 tttgtggata cggatgacga tgtgcgtatt attcgtcgta tcaagcgtga tatggaggag
     5641 cgtggccgta gccttgatag cgttattaac cagtacttag gtgtggtcaa accaatgtac
     5701 caccagttta tcgagtcaac taagcgttat gctgatatcg tcattcctga aggggttagc
     5761 aataccgtgg ctatcgacct gttgacgacc aagattgcaa agattttgga agaagctcga
     5821 aacagcaaat aatcacatgt ggaggccttg cctccttttt ctatttttcc ctcataatgg
     5881 tacataaatg gagattttta ggcatatttt tggtataata atacccatga aagagcaagt
     5941 aaatgaatag taggtggaga tggaaaagta tttatcggta acaactttga ccaagtatct
     6001 gaaaatgaaa ttcgataaag acccatactt ggaacgggtc tatttaactg gtcaagtttc
     6061 caactttcgt aaacgaccta ctcaccaata tttctcccta aaggatgacc atgcagttat
     6121 tcaagtgacc atctggtctg ggatttatca gaaattaggg tttgacctgg aagaaggaat
     6181 gaagatcaat gtgattgggc gtgtacaggt ctatgaacca agtggtagct actccatcat
     6241 cattgaaaaa gctgagcctg atggggttgg ggcgcttgcg attcagtttg aacaacttaa
     6301 gaaaaaattg acagaagaag gcctgtttca agaacgcttc aagcaagctc taccccaatt
     6361 ttctaagaga attggggtag taaccagccg tagtggagcc gttattcgag atattatcac
     6421 gaccgtcagc aggcgattcc caggtgttga catccttctt tatccgacca aggttcaagg
     6481 tgaaggggct gcggaggaaa ttgctagaaa tattgcgcgt gctaatcaac gggatgattt
     6541 ggatttgctc attattggtc gtggaggtgg ttctattgag gatctctggg cctttaacga
     6601 agaaattgtg gtacgagcta tttttgaatc tcgtttgcca gttatctcta gtgtggggca
     6661 tgagacggat gtgaccttgg cagattttgt ggcagatcga cgcgctgcaa cgccaacagc
     6721 ggcggctgaa ctggcaacac ctgtgaccaa gttggatgta ttagctcatt tgcaaaatca
     6781 ggaaaaacgg atggcaacgg cagtccgaaa tgttctatct aagaaacaag aggctttgaa
     6841 aaaatgcagt cagtctgtta tctttagaca acctgagcgc ttgtatgacg gttatttgca
     6901 acgcttggac caactgcaac tgcgtttgaa acaaagtttg cgaactcgga tttctgataa
     6961 caagcaatta gttcaagcaa gaactcatca attagtgcaa ttatcacctg ttaccaaaat
     7021 ccaacgctat caagaccgtt taggacagtt ggacaagctc ttaggtagcc aaatggcgtt
     7081 agtttatgac gccaaggttg ctgaggccaa gcgactttcg gaagctttgc tcatgttgga
     7141 tactagccga atcgtggcgc gtggttatgc tattgtcaaa aaagaagagt ccattgtaga
     7201 ttcggttgag agtttgaaga aaaaagacca agtaacgctt ttgatgcgag atggtcaagt
     7261 agaattagag gttaaagatg tcaaaacaaa agaaatttga ggaaaatcta gcagaactgg
     7321 aaaccattgt ccaaagtttg gaaaatggtg aaattgctct ggaagatgcg attactgcct
     7381 ttcaaaaggg catggtcttg tcaaaagagc tccaagctac gctggacaag gctgaaaaga
     7441 ccttggtcaa ggtcatgcaa gaagacggaa cagaaagtga ttttgaatga aaaagcaaga
     7501 aaaattagct cttgtcgagt cggctttgga agatttttat ggagaccagc agtttgcctc
     7561 tagtttacgg gagtctgttc tctattctat tcatgctggt ggcaagcgta ttcggccttt
     7621 tctcttgtta gaagttctgg aagccttgca ggttgccatc aaacctgctc acgcgcaggt
     7681 agctactgcc ttggagatga ttcatacagg gagcttgatt cacgatgacc ttcctgctat
     7741 ggatgatgac gattatcgaa gagggcggtt aaccaatcac aagaaattcg gtgaagctat
     7801 ggccattttg gctggagatg ccttattctt agacccatat gccttgattg cgcaggcaga
     7861 tttgccaagt cagatcaagg tggacttgat tgccaactta tcccttgctt caggtagtct
     7921 gggtatggtg gcagggcaag ttttggatat ggagggcgaa caccagcact tgtctctgga
     7981 agaacttcag actattcatg ccaataagac tgggaagtta ctagcctatc ccttccaagc
     8041 ggcagctatt atagctgaat tgtcacctga aatgcaggtg aagctgaaaa ctgtgggtga
     8101 attgattgga cttgcttttc aagtcagaga tgatgtactg gatgtgacag ctagttttga
     8161 ggaaatcggc aagacacctc aaaaggatct gcaggcagaa aaatcaacct atcctgcctt
     8221 gttgggcttg gaagagtcca ttgccttttg taaccaaacc ctggatcaag ctaatgaaaa
     8281 attagaagaa attgcccagc aacttccctt tgaaacagaa tcgattgtaa gtgtagtaga
     8341 aagtttgaga atcaatggct aaggaaagag tggatgtact agcttataaa caggggttgt
     8401 ttgaaacgag agagcaggcc aagcgaggtg tgatggctgg cctagtcgta gcagtcctta
     8461 atggagaacg gtttgacaag ccaggagaga aaattccaga tgacaccgaa ttaaaactca
     8521 agggggagaa actcaagtat gtcagccgtg gtggtttgaa actggaaaag gccttgcagg
     8581 tctttgattt gtcggtggat ggcgcgacta cgattgatat cggggcctct actggaggtt
     8641 ttaccgatgt catgctacag aatagtgcca agttggtctt tgcagtcgat gttggtacca
     8701 atcagttggc ttggaaatta cgccaagacc cacgagttgt cagcatggag cagttcaatt
     8761 tccgctatgc tgaaaagact gatttcgagc aggagccgag ctttgccagt attgatgtga
     8821 gtttcatttc ccttagtctg attttgccag ccttgcaccg tgtcttggct gatcaaggtc
     8881 aggtggtagc acttgtcaaa cctcagtttg aggcaggacg tgagcagatt gggaaaaatg
     8941 gaattattcg agatgctaag attcatcaga atgtccttga atctgtaaca gctatggcag
     9001 tagaggcagg tttttcagtc cttggcttgg acttttctcc catccaaggt ggacatggaa
     9061 atattgaatt tttagcgtat ttgaaaaaag aaaagtcagc aagcaatcag attcttgctg
     9121 agattaaaga agcagtagag agggcgcata gtcaatttaa aaatgaataa aaaagaaaga
     9181 cttgaaaaaa ttagaagatt ggtgacagat tatcaaatcg gcacgcaaga agaaattgta
     9241 gaacatttga aagaagcagg tatcactgcc actcaggcga cggtatcccg agatatcaaa
     9301 gagttaggta ttgtcaaaat tcctttgaga gacaacacct atgtctacga attaccaaaa
     9361 tcaatcgtaa aaagtctgca actggctgaa gacaatatcg aatcggctga attgatggat
     9421 aagatgatca atctccaagt tattccagga aatacggctt ttgtaaaagc tcagttaatc
     9481 gatacttttg cagacaagat ttttagttgt ttgactgatg atagctcgat tttagtcatt
     9541 gccagaagtg gaagtctagc agaggaaatc tttgaacaag taaaaaattg gtaggtctat
     9601 atgttacttg aaatttcgat aaaaaacttt gccattattg aggctatttc cctcaatttt
     9661 gaaaagggga tgactgtcct gactggtgaa acgggtgcag ggaagtcgat tatcattgat
     9721 gccatgaata tgatgttggg agctcgtgcg acgacagatg ttattcgtca tggtgcacca
     9781 aaggcagaga ttgaggggct tttctcagtt gagaatagtc gccttttaca ggaaattttt
     9841 gatgagcaag gtttagaatt aggtgatgaa attatcatcc gtcgggaaat cttgcaaaat
     9901 ggtcgtagta tcagccgtgt aaatggtcag atggttaatc tgtctgtttt gcgagctatt
     9961 gggcaacatc ttgtagatat tcatggtcag catgaccatg aggagttaat gcgtccccaa
    10021 ctgcatatcc agatgttgga tgaatttggt gatgccgctt tttgggactt gaaagaaacc
    10081 tatcaaacga gttttgatgc ctatcggaaa atgcgcaagc aggttctgga agtcaagaaa
    10141 aaccaacagg aacacaaggc acgtatcgaa atgttggaat ttcaaatggc agagattgag
    10201 gcagcaaact tgcaggctgg agaagacttg gctctcaatc aagagcgaga taaactcctc
    10261 aaccacaaaa atattgcgga tacactgacc aatgcctaca gtatgttgga caatgaagat
    10321 ttttcaagtc tggccaatgt tcgttcagct atgaatgaca tggaaagtgt cgaagagtat
    10381 gatcctgaat accgtgaaat ttcaagctct ctgtctgaga cctactatgt tttagaagac
    10441 attagcaaac gtttggaagc tatcattgag gaccttgatt ttgatggcaa tcgcctgatg
    10501 caggttgaga atcgtttgga cctccttcat actattactc gtaagtatgg tgggactgtt
    10561 gatgatgttt tgctttattt tgccaagatt acggaagaat acaatctctt gacaggcaat
    10621 aatctttcgt ctgaggacat ggaagcagag cttaagaagt tggaagtcaa tcttgtcaat
    10681 ttggcaggtc aacttgcttc tgctcgtcat aatttggctc agcaactcga agctgagatt
    10741 aaacaagaac tgcaagatct ttatatggaa aaagcccagt ttcaggttcg ttttagtaag
    10801 ggaaaattca gtcgtgaggg aaatgaaatg gttgagtttt atatttcaac caaccctgga
    10861 gaagacttta aacccttggt taaggttgct tctggagggg aattatctcg tctcatgtta
    10921 gccattaagt ctgccttttc acgtaaagaa ggcaagacta gcattgtctt tgatgaggtg
    10981 gatacgggag tttcaggtcg tgttgctcaa gctattgctc agaaaattca taaaattggt
    11041 cagcatggtc aggttttggc tatctcccat ttgccacaag taattgcgat tgcagattat
    11101 caattcttta ttgagaagat tagtaatgac cattcaacgg tttcgactgt tcgtctcttg
    11161 acggtcgaag agcgagtgga ggaagttgcc aagatgttgg caggtgatga tgtgacagaa
    11221 gcagccctga cgcaagccag agaattgttg agaaacaggg agaaataaga tgacagatta
    11281 ttatgtaatt ggagatgttc acggaaaagc tgggatgctg gaagaccttc tcaaaacctg
    11341 ggatggtcag ccccagttgc tctttctagg tgatttgatt gacagaggtg aggatagtca
    11401 ccgtgttctt gaaatggtca aggacttggt ggacaatcaa ggggctatct gtttgtccgg
    11461 aaaccacgag tatatgtttt tgacttggct agatgaccca gaagaaagtt atgaccatta
    11521 tcgtcgcaat ggtggagata caaccattaa ctctatccta ggtcgtccct tggatgcacc
    11581 agttgatgga gtagaagatg ccaagcgggt tgccgctgaa gcgtcagact tggtcgaatt
    11641 cattcgtcaa atgccatttg tagtagagac agacaagtat atatttgttc acgcaggtat
    11701 tgatttgact ttggacgact ggcatgaaac aacagattat aaaaaagtat ggcttagaaa
    11761 accattccac ggagctgaaa atcatactgg aaaaaccatt gtcttcggtc atacaccggt
    11821 ctatggtttg ttacagcaag aacgcggtac atctgagctt tggactacag aagatggcaa
    11881 gattggaatg gatggaggag ctgtttatgg tggtgtcctt cacgggattg tctttacaga
    11941 ccaaggaatg acagaacacc actttatcga aaatgacggc tttgtcgctg aagattagta
    12001 ctcctagcag ggtatggtct tgtcaaaata tcaaaaacaa tttataataa atagataccc
    12061 tgaaaggaag agaatcatga acttagaaga attgaaaaaa cgacagagga agatccgtaa
    12121 cttctctatt atcgcccata ttgaccacgg gaaatcaact ctagcagacc gcattttgga
    12181 aaaaacagag acggtttcaa gtcgtgaaat gcaggcccag cttttggata gcatggatct
    12241 agagcgggaa cgtggaatta ctattaagtt gaatgccatc gagttgaatt acactgcaaa
    12301 agatggggaa acttatattt tccacttgat tgacacacca gggcacgttg actttaccta
    12361 tgaagtttca cgttcgctag ctgcctgtga gggtgctatt ttggtggtcg atgcggctca
    12421 aggaattgag gctcaaactc ttgccaacgt ttatctggct ttggataatg atttggaaat
    12481 catgcctatc attaataaaa ttgacctgcc ggctgcagat ccggagcgcg tgcgtacaga
    12541 gattgaagat gtgattggtt tggatgccag tgaagcagtt ttggcttctg ccaaggctgg
    12601 tattgggatt gaagaaatcc tcgagcaaat tgtagaaaaa gtaccagcac caacgggtga
    12661 tgtgacggcg ccacttaagg ccttgatttt cgactctgtt tacgatgctt accgtggggt
    12721 tatcctccaa gtgcgtgtca tggacggagt ggtcaaacct ggtgataaga ttcagctcat
    12781 gagcaatagt aagacctttg atgtggccga agtcggtatt tttacaccaa aagcggttgg
    12841 tcgtgatttc cttgcgactg gtgatgttgg ttacattgcg gcgtctatca agacggttca
    12901 ggatactcgt gtgggtgata ccgttacctt ggcaaccaat cctgcggcag aaccattaca
    12961 tggttataag cagatgaatc ctatggtctt tgcgggtctc tacccaatcg aatcaaacaa
    13021 atacaatgac ctgcgtgaag cgcttgaaaa attgcaactg aatgatgcta gtcttcagtt
    13081 tgaaccagaa acatctcagg cacttggatt tggtttccgt tgtggatttc ttggacttct
    13141 ccatatggat gttatccagg aacgtttaga gcgtgagttc aatattgacc tcatcatgac
    13201 agctccgtct gttatttaca aagttaattt gaccgacggt gagtctatgg atgtgtctaa
    13261 cccatctgag tttccagacc caactaagat tgcgaccatt gaagagcctt atgtcaaggc
    13321 gcaaatcatg gtaccacagg agttcgtcgg agcagtaatg gagctagctc agcgtaagcg
    13381 tggggacttt gtgactatgg actatattga tgataaccgt gtcaatgtta tctatcaaat
    13441 tcctcttgct gaaattgtct ttgacttctt tgataaactt aagtcttcga cacgtggtta
    13501 tgcaagcttt gactacgaat tgtcagaata tcgcccatct aagctggtga aaatggatat
    13561 acttctcaat ggagacaagg tggatgccct cagctttatc gttcacaagg actttgccta
    13621 cgaacgtggg aaactcatcg ttgataaact caagaaaatc atccctcgcc aacaatttga
    13681 agttccaatc caagcggcta ttggacacaa gattgtcgct cgtactgata tcaaggccct
    13741 tcgtaagaac gtacttgcta aatgttatgg tggtgacgtt tctcgtaagc gtaaactcct
    13801 tgaaaaacaa aaagctggta agaaacgcat gaaatccatc ggatcagttg aagttccgca
    13861 agaagccttc ctcagcgtct tgagtatgga tgaagaataa gttatcagaa atgcctttcc
    13921 ttgttcacag gaaatttata taaaaatcaa tagtaaagca ggcttagatg gtctgctttt
    13981 ttagttttta tactcttcga aaatctcttc aaaccacgtc agcgtcgcct taccgtatgt
    14041 atggttactg acttcgtcag tttcatctac aatctcaaaa ccatgttttg agctgacttc
    14101 gtcagtttca tctacaacct caaaaccatg ttttgagctg acttcgtcag tttcatctac
    14161 aacctcaaaa ccatgttttg agctgacttc gtcagtctta tctacaacct caaaactgtg
    14221 ttttgagctg acttcgtcag tttcatctac aacctcaaaa ccatgttttg agctgacttc
    14281 gtcagtttca tctacaacct caaaaccatg ttttgagctg acttcgtcag tcttatctac
    14341 aacctcaaaa ctgtgttttg agtaacctgc ggctag
//
LOCUS       DAVGJL010000047        13603 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.047, whole genome shotgun sequence.
ACCESSION   DAVGJL010000047 DAVGJL010000000
VERSION     DAVGJL010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13603
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.047"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(<1..507)
                     /locus_tag="VVE42_001113"
     CDS             complement(<1..507)
                     /locus_tag="VVE42_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEU3073316.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILTENLETNLSVLDIGTGSGAIALALAKNRPDWSVTAADVSQEALDLASENAKNQNLQ
                     IFFKKSDCF"
     gene            complement(507..1586)
                     /gene="prfA"
                     /locus_tag="VVE42_001114"
     CDS             complement(507..1586)
                     /gene="prfA"
                     /locus_tag="VVE42_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEU3073317.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEEMAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLEELNK"
     gene            complement(1596..2075)
                     /locus_tag="VVE42_001115"
     CDS             complement(1596..2075)
                     /locus_tag="VVE42_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU3073318.1"
                     /translation="MTIELRDVTMENYFDVLNLDVKEYQKQFIATNAISLAEAYVYTK
                     NGDFVAPLAVYDNDVIIGFVMIAYDKKIGISSGNYLLFRFMIDKNFQNQGYFKPIMDK
                     VLDYVRTAPAGLSNKLWLSYEPENEQARFCYLSYGFKETGEISENEVVAIYDLTIEK"
     gene            complement(2065..2664)
                     /locus_tag="VVE42_001116"
     CDS             complement(2065..2664)
                     /locus_tag="VVE42_001116"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEU3073319.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GVGYVSSRIGMKRPALAIEETTDIFGYIRDLPEKPYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFAPMITSNKEQNYDN"
     gene            2787..2969
                     /locus_tag="VVE42_001117"
     CDS             2787..2969
                     /locus_tag="VVE42_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEU3073320.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAVVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            3106..4479
                     /gene="mnmE"
                     /locus_tag="VVE42_001118"
     CDS             3106..4479
                     /gene="mnmE"
                     /locus_tag="VVE42_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEU3073321.1"
                     /translation="MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIAQKIFKGKDL
                     NKVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNTRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTKLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEADLVLLVLNASEPLTAQDRQLLEISQDTNRII
                     LLNKTDLPETIETSKLPEDVIRISVLKNQNIDKIEERINNLFFENAGLVEQDATYLSN
                     ARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLF
                     SQFCLGK"
     gene            complement(4526..5461)
                     /gene="dapA"
                     /locus_tag="VVE42_001119"
     CDS             complement(4526..5461)
                     /gene="dapA"
                     /locus_tag="VVE42_001119"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEU3073322.1"
                     /translation="MSYQDLKECKIITAFITPFHEDGSINFDAIPALIEHLLAHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKVVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKAIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     YPNIIGVKECTSLANMAYLIEHKPEEFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFTAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAVLNYMGFEAGPTRLPL
                     VPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(5517..6593)
                     /locus_tag="VVE42_001120"
     CDS             complement(5517..6593)
                     /locus_tag="VVE42_001120"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEU3073323.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEESTLPIDKIRYLASARSAGKS
                     LKFKDQDITIEETTETAFEGVDIALFSAGGSTSAKYAPYAVKAGVVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGVKPCDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIAAF
                     PGAVLEDDVAHQIYPQAINAVGSRDTFVGRIRKDLDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            complement(7179..7251)
                     /locus_tag="VVE42_001121"
     tRNA            complement(7179..7251)
                     /locus_tag="VVE42_001121"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7216..7218),aa:Thr,seq:ggt)
     gene            complement(7351..7860)
                     /locus_tag="VVE42_001122"
     CDS             complement(7351..7860)
                     /locus_tag="VVE42_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEU3073324.1"
                     /translation="MKKLTIRDVADIAIVAAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLLDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIRKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     regulatory      complement(7858..7954)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            7983..8414
                     /locus_tag="VVE42_001123"
     CDS             7983..8414
                     /locus_tag="VVE42_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEU3073325.1"
                     /translation="MKKPIQKFFNNEILAYLFFGLATTLVSILSRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLFLTFLFVTQFP
                     HIIGQFVTENIDKINAIETIIAQIFIIIINYILSKVYIFRK"
     gene            8496..8873
                     /gene="mscL"
                     /locus_tag="VVE42_001124"
     CDS             8496..8873
                     /gene="mscL"
                     /locus_tag="VVE42_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEU3073326.1"
                     /translation="MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKEE
                     KTAEKKPTELEVLQEIKALLEKK"
     gene            complement(8915..10009)
                     /gene="hemH"
                     /locus_tag="VVE42_001125"
     CDS             complement(8915..10009)
                     /gene="hemH"
                     /locus_tag="VVE42_001125"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEU3073327.1"
                     /translation="MKKAILMMTFGSPEEITFEGVADFFTNIRRGVRPQDHEIQTLYD
                     NYVRIGGTPLQKITRQEVALVEARLGNEYSVYFANKFSSPFIPDVIGQMEADGIEQCI
                     CLILEPHYSFYSVMGYEKFLESKQIQFLVIKDWYQEEALLNYWADEIAKILKEEVKQD
                     SFKVIFSAHSVPIFALDFGDPYIDQIFENSKLVAEKLGLSSKQYTNTWQSESDIGIPW
                     IKPDVLEYLREQTEHPDHYIFVPISFISEHIEVLFDNDVECYDLCQEFGVNYHRPPMP
                     NTDSRLIDALVNTIRINENQEFKEFLPEEETFDELVPSDETKNILAESEDLQMPEFVK
                     KLIEKKGRENVKMPYLIKKMLEKAGKLPKE"
     gene            10164..11387
                     /gene="pepT"
                     /locus_tag="VVE42_001126"
     CDS             10164..11387
                     /gene="pepT"
                     /locus_tag="VVE42_001126"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEU3073328.1"
                     /translation="MTYPNLLDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDG
                     GVIKLGNSGFKLDPADFKSLEKYPGQTLITTDGTTLLGADDKSGIAEIMTAIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKGQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEA
                     RASYIIRDFEKDAFEARKASMQSIADKMNEELGSNRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKAVMEDLGITPIIEPIRGGTDGSKISFIGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVAYKG"
     gene            complement(11423..12052)
                     /locus_tag="VVE42_001127"
     CDS             complement(11423..12052)
                     /locus_tag="VVE42_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073329.1"
                     /translation="MNNANQLLEQIHEEGIRQSLAEEVENLKAATNKADADLDEVNSQ
                     VKDVLTRIASALQQEKENAEQDSQTLVLYQKLYDILMSLHSYLESNNGSDADFDKVDA
                     LLDQLSAKSKDKTALLESTKAILVLNHQIQSKASASEETSPARNAEANSDNTSAENQP
                     NATAESNIETASDENKPSNTRDSKPAESTSENKTTESSTTTGNQEKPVE"
     gene            12017..12238
                     /locus_tag="VVE42_001128"
     CDS             12017..12238
                     /locus_tag="VVE42_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073330.1"
                     /translation="MDLFQELICIVQKTFYFFLSICRRLLVAIYHVLLKQESYNPRLQ
                     GLTEIRNPDKTMFVQDAIRFAQQHGFNIL"
     gene            complement(12509..13521)
                     /locus_tag="VVE42_001129"
                     /pseudo
     CDS             complement(12509..13521)
                     /locus_tag="VVE42_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669441.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
BASE COUNT         4006 a         2856 c         2498 g         4243 t
ORIGIN      
        1 aaaacagtca gattttttaa aaaatatttg aagattttga tttttagcat tttcgctagc
       61 taaatctaaa gcctcttgag aaacatctgc tgctgtcact gaccaatctg gtctattttt
      121 tgctagagcg agagcaatag ctccactacc tgttccaata tccagaactg aaagattcgt
      181 ctcaagattt tcagttagaa taagctccac taactcctct gtttctggac gaggaatcaa
      241 aacccgttca tccaccttta aatgcattcc ataaaaatct gcctgtccaa tgatgtactg
      301 agctggcttg tgagctgcta gttgctggta aatatcttct acaaattgtt tttcttcctc
      361 tgttgtcacc tcctgctgga gggcaaaaat aaagtctgta aaagatagat ttttcagact
      421 acgatagaca aaagagaggc tttccgcttc ctctccttgt cttatcaact cttcttcaaa
      481 atttgaaaat aattgagcta atttcattat ttgtttaatt cttctagttt ttgtgtttgg
      541 tcataaagca ccaaggcatc cacaacttcg tccaatttac cagacaaaat cgtatctagt
      601 ttttggaggg tcaagccgat acggtggtct gtgacacggt tttgtgggaa gttataagtt
      661 cggatccgtt ctgaacggtc accagtaccg attgtcgact tacgctcagc gtcttgttca
      721 tcctgagcaa tctgagcaaa gtggtcagcg acgcgtgcac ggataatctt catagccttc
      781 tcgcggttct tctgctgggt acgttcttcc tgcatctcaa ccttgatatt ggttggcaag
      841 tgaacgatac gaacggcagt cgcaacctta ttgacgttct gtccaccagc accagaggcg
      901 tgatagatgt cgacacgaag gtcttttgga tcaatgtcgt attcaacctc ttcaacttct
      961 ggcataacaa gaactgtcgc tgtcgaagta tgaacacggc cttggctttc tgtcacagga
     1021 acacgttgca cacggtgggc acctgattca tacttaagct tagagtatac agactgacct
     1081 gaaaccatag caaccacttc tttaaaacca ccgacaccat tcatagaggc ttccatgact
     1141 tcaaagcgcc aaccttgggc ttccgcatac ttttggtaca tagttagcaa atctccagcg
     1201 aaaagtgccg cttcgtctcc accagctgct ccacggattt caaggatgat attcttgtca
     1261 tcgtttggat cctttggaag gagcaaaatt ttcagttttt cttcatattc ttctttttca
     1321 gccttggcat ctttgagttc ttgcttggcc atttcttcca agtccgcatc tccgcctgat
     1381 tccttaatca tctcttcggc atcgacgata ttttgaagga cttgtttata ctcacggtag
     1441 gctattacgg tgtcacgatt ggaagcttct tcttttgaaa gctccataaa acgcttggtg
     1501 tctgaaacga catcagggtc actcagcaat tctcctagtt cttcataacg gtcttctaca
     1561 gcttgtagtt gatcatagat gttcattttt tctccttatt tctcaattgt taaatcatag
     1621 attgctacta cttcattctc ggatatttcc ccagtttctt taaatccata actgagataa
     1681 caaaatcttg cctgttcatt ttctggttca taagacaacc aaagtttatt gcttaaacct
     1741 gctggcgctg ttcgaacata gtctagtact ttatccataa ttggtttaaa atatccttga
     1801 ttttgaaaat tcttatcaat cataaaacga aatagtaaat aatttccact actaattccg
     1861 atctttttat cataagctat catcacaaaa cctataatta catcattatc ataaactgcc
     1921 aatggagcta caaaatctcc atttttagtg tagacgtatg cttcagctaa actaattgcg
     1981 ttggttgcaa tgaattgttt ttgatattcc ttgacatcca aatttaaaac atcaaaataa
     2041 ttttccattg taacatctct tagttcaatt gtcatagttt tgctccttgt tagaggttat
     2101 cattggcgca aaataatgtt tacggcaaac cgagatatag gtttcattac caccaatctg
     2161 gatctgttct ccatcgtaaa cgggcagtcc atcctgtgtt cgcaacacca tggtcgcctt
     2221 tttcttgcaa tactgacaga tggtcttgat ttcgtcaatc ttgtccgcta aaagcaagag
     2281 atatttggaa ccttcgaaca attcattgcg aaagtcattt ttcaagccaa aagccatgac
     2341 gggtatgtct aactcgtcca caacacgagc taggtcgtaa acatggtggc gtttaagaaa
     2401 ctgggcttca tcgaccaaaa cacagtaagg tttttctggt aggtctcgga tatagccaaa
     2461 gatatccgtt gtttcctcaa tcgcaagggc agggcgtttc atgccaattc gactcgacac
     2521 atagccaaca ccgtcacgcg tatccagagc cgaggtcata atgacaacac cttttccttg
     2581 ctcctcgtag ttataggcca ctttgagaat ctcaatcgtt ttaccagagt tcatggtccc
     2641 ataacgatag tacaactgtg ccatgtttct tgcttcacgt ccatttctaa atttttgcta
     2701 cattctagta tatcataatt ttcttaagct ttaaacggca aaatgtggta aaatggaaga
     2761 aatcaaaact agtggaggaa gctattatgc catttgtacg catcgattta tttgaaggac
     2821 gcacgctcga gcaaaagaaa gctcttgcta aggaagtaac ggaagcagtt gtccgcaaca
     2881 ctggagcccc tcaatctgct gtccatgtca tcatcaacga catgccagaa ggaacttact
     2941 tcccacaagg ggaaatgcgt actaaataag ctagcttaag cagaattgct taggcttttt
     3001 caatctccaa gtagcattca ttgaagaaat atcctaaatt tgttacaatt tgaaaagaaa
     3061 cttggagaat ttccaagaaa agagctatta attaaaggaa acattatgat tacacgtgaa
     3121 tttgatacca tcgctgctat ctctactcca ctaggtgaag gggctattgg tattgtccgc
     3181 ctgagcggaa cagacagttt tgctattgcg caaaagattt ttaaaggaaa agacttgaac
     3241 aaggttgcca gccacactct caactacggt cacattattg atcctctgac tggtaaagtc
     3301 atggacgagg tcatggtagg agccatgaaa tctccaaaaa ccttcactcg tgaagatatt
     3361 atcgagatta acacccacgg tgggattgcg gtgaccaatg agattctcca gctagctatc
     3421 cgtgaaggag ctcgattggc tgaacctggt gaatttacca aacgtgcctt cctaaacggt
     3481 cgcgtagatt tgacacaggc tgaggcagtg atggacatca tccgtgccaa gactgacaag
     3541 gccatgaaca ttgcagttaa gcaattagac ggctcccttt ctgacctcat taacaatacc
     3601 cgtcaagaaa tcctcaatac acttgcccaa gttgaggtca atatcgacta tcctgaatat
     3661 gatgatgttg aggaagctac tactgctgtt gtccgtgaga agactatgga gtttgagcaa
     3721 ttgctaacca agctccttag gacagcccgt cgcggcaaaa tccttcgtga aggaatttca
     3781 acggctatca ttggacgtcc caacgttggg aaatcaagcc ttctcaacaa cctcttgcgt
     3841 gaggacaagg ctatcgtaac agatatcgct gggacaacac gagatgtcat cgaagagtac
     3901 gtcaacatca atggtgtacc tctcaaattg attgatacag ccggtattcg tgaaacagat
     3961 gacatagtgg aacaaatcgg tgttgagcgt tcgaaaaaag ccctcaagga agccgacttg
     4021 gttctactag tgctaaatgc cagtgaacca ctgactgcgc aagacagaca acttcttgaa
     4081 attagccaag ataccaatcg cattattcta cttaataaaa ccgacctgcc agaaacgatt
     4141 gaaacttcga aactacctga agacgttatc cgtatttcag tccttaaaaa ccaaaacatc
     4201 gacaagattg aagagcgaat caacaacctc ttctttgaaa atgctggctt ggtcgagcaa
     4261 gatgctacct acttgtcaaa cgcccgtcac atttccctga ttgaaaaagc agttgaaagc
     4321 ctacaagccg ttaatcaagg tcttgagctg gggatgccag ttgatttgct tcaagttgac
     4381 ttgactcgta cttgggaaat cctcggagaa atcactgggg atgctgctcc agatgaactt
     4441 attacccaac tctttagcca attctgttta ggaaaataag aaaaatccat gatccttcat
     4501 tcggtcatgg attttattgt ctttattagt aatctggtct taagacccct gttacagttg
     4561 ccttagttgc ttcgtagtcg ccatctacga caaccttgat aatgcgtttg gcatcttctt
     4621 ctggtgctgg aacaagaggt agacgagtgg gtccagcttc aaatcccata tagttaagaa
     4681 ctgccttaac tggagcagga cttggataag agaagagagc attaacctta ggaatgaatt
     4741 tacgctgaat tgctgcggct ttcttcatat cgctttctgc aatggcagta aacatctcgt
     4801 gcatttcatc cccatttgta tgagaggcaa cagaaataac cccatccgcc ccaaggttca
     4861 tggcatggaa agcatctcca tcctcacctg tataaatcaa gaactcttca ggcttgtgct
     4921 caatcaaata agccatatta gccaagctag tacattcttt gacaccgata atatttggat
     4981 agtcagccaa gcgaagcatg gtttctggag tcaattcgac aactacacgc cctggaatgt
     5041 tatagataat aattggtagg tcagaagcat ctgcaatagc cttaaagtgc tgatacatcc
     5101 cttcttgaga aggtttgttg tagtaaggaa caatagcaag cccagctgcg aaaccaccaa
     5161 attccgctac ttctttgaca aactcaatag agtcacgcgt atcattagta cctacacccg
     5221 caatcaaagg aacgcgtcca ttgacaacct tttgtacagc cgcaaacaac tccaactcct
     5281 catcgtgggt caaagttgga ctctcagcag tcgttcctgc gagaagaatt ccatccgtat
     5341 gatgggccaa taaatgctca atcaaggctg gaatagcatc aaagttaatg gaaccatcct
     5401 catggaaggg ggtaataaag gctgtaatga ttttacattc ttttaaatct tgataagaca
     5461 cgaaaacacc tctctatttc aaagaaggag ttcatctgaa ctcctaaacg atatgactat
     5521 tttaattcaa atttcaattc ggctgttgga cgaaccaatc cacgttcatg aagagtttca
     5581 gcaatctgaa ctgagttcca agcagcacct ttgagaaggt tatctgaaac aacccacatg
     5641 tgaattcctt tttctgcatc caagtcttta cggatacgac caacaaaggt atcacgcgaa
     5701 ccaactgcat tgatggcttg aggatagatt tgatgagcta catcatcttc aagaacagca
     5761 cctgggaagg ctgcgatagc tgcttttact tcttcgattg gagccacttc ttttgtttcg
     5821 atataaacag actcagagtg agctgacaag actggaatac gcacacatgt tgcagatact
     5881 gcaatgctat catcttccat aattttctta gtttccttgg tcatcttcat ctcttcgtac
     5941 gtgtaatcat tatcagtgaa aacatcaatt tgtggaagag cgttaaaggc gataggataa
     6001 tgtttcttgt caccacctga aggcaaaatt tccgcatgca aatcacatgg tttcacacca
     6061 tcattcaaga cttcacgaag ttcacgttgt gtctcaagaa ttgctcccat accagcacct
     6121 gaaacggctt gataagttga aacaatgata cggtccaagc cccatttttg gcgaaccggc
     6181 tcaagagcca ccatcatttg gattgttgaa caattagggc aggcaatgat tccgttgtga
     6241 gcatcaagtg catgagcatt gacctctgga acaaccaaag gaacatctgg attttgacgg
     6301 aaataagatg tattatctac tactaccacg ccagctttta ctgcgtatgg tgcatactta
     6361 gctgatgtag aaccacctgc tgaaaagaga gcaatatcaa ctccttcaaa agctgtttca
     6421 gtcgtttctt caatcgtaat atcttgatct ttaaatttca atgacttacc tgctgaacgt
     6481 gcagaagcaa ggtaacggat tttatcgatt ggaagtgttg attcttccaa catttttatc
     6541 atctgagcac cgacagcacc tgtcgcgccg actacagcaa ctgtatatcc cataaataac
     6601 ctctttcgga attttctaaa aatttctata atagaagtat tatactactt tttccatgaa
     6661 ttgcaaagct ttttcatcat tttttggaaa ataaaaatcc ccaatcgcta gactagggat
     6721 taagaggcat cttcatgtga tgagcaggtt cacacaactc atcaaggtcc gctcctgcgt
     6781 tatgacctcc ttatgctcaa tagtagcccg aagactacct atcgactcat cgcatctact
     6841 attctactaa agagaatgac ttttgtcaac agacgaaact ctttatactc ttcgaaaatc
     6901 tcttcaaact tcgtcagcgt cgccttgccg tatgtatggt tactgacttc gtcagtttca
     6961 tctacaacct caaaaacatg ttttgagctg acttcgtcag ttctatctac aacctcaaag
     7021 cagtgctttg agcaacctgc ggctagtttc ctagtttact ctttgatttt cattgagtat
     7081 tagttttatt tacacaggtg cataagaaat atattggaaa aattgggggc agacataaca
     7141 aaaacccttg taaatcaagg gcttttctgt ctatttaatg ctaattgccg ggattgaacc
     7201 ggcgacctca tccttaccat ggatgcgctc tgccaactga gctaaatcag cttacttaag
     7261 aagtatacta tattcagaaa aacttgtcaa gtttccttag aaattttcca taggaaaaag
     7321 ccaggtaaga actacctagc ttatcttctt ctatttactt aaatcaattc gacgaccaat
     7381 tttaccaata ataatcgctc caataatcaa tgaagcaaat tcaccgattc ctgttgtaaa
     7441 ccaagtaagg aagaatggta attctgctac aatatttaac tctgcagcga tagtaaacat
     7501 ggaaattgaa aagaggattg aaaagaagaa atgatctttt cgaatcaatc cgttaaagag
     7561 gtaatccttg ctatattttg caaaaagcca aacacctaaa ctgaggaata ctaaggttga
     7621 accaccacca acaaagacat ctagcagtcc gaagctaaag aaattagcaa tcatacaacc
     7681 gatggtaact ccgatgatgt acttaggatt gtaaaaagcc ataaagttca tcatttctga
     7741 aatacgaaac tgataagcac catagctgat ggcatttagg ggcggtgtga cagtcaaaac
     7801 gacgtagata gcagcgacga ttgcaatatc tgcaacatca cgaatagtta attttttcat
     7861 cttttctcct ttggcggtta tccgcgtgta atatgcttgg tgaaagaagc taagcaccaa
     7921 gggttgattc attcaacctt actagtatag cacaatagcc tgctttatgc tatacttaaa
     7981 gcatgaaaaa acctattcaa aaatttttta acaatgaaat cttagcctat ctcttttttg
     8041 gccttgcgac aactctagtt tcgattctct cacgactagt catttatcaa ctaagtcatc
     8101 aggaactcct tgctacggcc ttggcaaata ttatcggtat cttatttgcc tttatcacaa
     8161 atgatacgat tgtatttaag caaaaaaaga aaaatagact tatccgttta gtgaaatttt
     8221 ctcttgctcg cctttctact tttctgttag acttgttttt aacttttctc tttgtaaccc
     8281 aatttcctca catcataggg caattcgtaa ctgaaaacat tgataagata aatgctattg
     8341 aaacaataat tgcacaaata ttcattatta tcatcaatta tatcttgagc aaggtctata
     8401 tttttagaaa ataatgctcc atttatgagc cttttaattg ccttctataa tgatttagga
     8461 tacaatgaaa cacgaaacat tgaaaggaaa acagtatgtt aaaaaatcta aaatcgttct
     8521 tgcttcgagg aaatgttatt gaccttgctg tcggtgttgt cattgcctct gcttttggtg
     8581 ctatcgttac ttcacttgta aacgacatta tcactcctct tattttaaat cccgctttga
     8641 aagctgctaa agttgaacgt atcgctcaac tttcttggca tggagtcggc tatggtaact
     8701 tcttaagtgc tattatcaat tttatctttg tgggtaccgc cctcttcttt attatcaagg
     8761 gcattgaaaa agcacagaaa ctgactggca taaaggaaga aaaaactgcc gaaaaaaaac
     8821 caaccgaatt ggaagtcctt caagaaataa aagctctcct tgagaaaaaa taatcgatta
     8881 aaaggatcta gcgcaaagct aaatcctttt ttctttactc tttgggtaac ttgcctgctt
     8941 tttcaagcat ttttttgata agataaggca tcttaacatt ctcccgccct tttttctcaa
     9001 tcagtttttt cacaaattct ggcatttgta aatcttcaga ttcagccaaa atgtttttcg
     9061 tctcgtctga aggaactaac tcatcaaagg tctcttcttc tgggagaaat tccttaaact
     9121 cttgattttc attgattcta attgtattga ccaaagcatc aatcaaacga gaatctgtat
     9181 ttggcattgg tggacgatgg tagtttaccc caaattcctg acataagtca taacattcca
     9241 catcattgtc aaacaagacc tcaatatgct cactgatgaa actaataggt acaaaaatat
     9301 aatggtctgg atgttctgtc tgttctctga gatactccaa aacatctggt ttaatccatg
     9361 gaataccaat atcactctca ctctgccaag tgttggtata ttgttttgaa ctcaagccca
     9421 gtttttcagc gactagcttg ctattttcga aaatttggtc tatataagga tcaccaaagt
     9481 ccaaggcaaa aataggcaca ctatgggctg aaaagatgac tttaaagctg tcttgcttta
     9541 cttcttcttt taaaatctta gcaatttcat ctgcccaata gtttaagagc gcttcttcct
     9601 gataccagtc cttaatgacc aaaaattgaa tttgtttgct ttctaaaaat ttttcatagc
     9661 ccatgacaga gtagaaagaa taatggggct ccaaaatcaa gcaaatacac tgttcaattc
     9721 cgtctgcttc catttgaccg atcacatctg gaataaaggg actggaaaac ttattagcaa
     9781 agtagacact gtattcattc cccagcctag cttctaccaa ggcaacctct tgacgggtaa
     9841 ttttttgcag aggcgtgcct ccaattcgta cataattatc atagagagtc tgaatctcgt
     9901 ggtcttgggg tctcacccca cgacgaatat ttgtgaaaaa atcagctaca ccttcaaagg
     9961 taatctcttc tggtgaaccg aaggtcatca ttaaaattgc ttttttcatg actgcttcct
    10021 tgtgatgttt ttatcaagtt tattttatca ttaattcatc gataagtaaa cgctaacata
    10081 gggacattct caatcttctg tcttttgttt ttctcttctt tctatgatac aatggaaaaa
    10141 ataaattcaa aaggagtttt tttatgactt atccaaatct cttggaccgc ttcttaacct
    10201 acgttaaggt caacacgcgc tctgatgaac actctactac tactccaagt acacagagtc
    10261 aggttgactt cgcaacaaat gtcctaattc ctgaaatgaa acgtgttgga ctgcaaaatg
    10321 tttactatct accgaatggt tttgctattg gaaccttgcc agccaacgat ccgtctttaa
    10381 cacgtaagat tggttttata tcgcacatgg atactgctga ttttaatgct gaaggagtca
    10441 atccacaggt aattgaaaac tacgatggtg gtgtgattaa actagggaat tctggtttca
    10501 aactcgatcc agctgacttc aagagtcttg aaaaatatcc aggacaaacg ctcatcacaa
    10561 cagatggaac aaccttgcta ggtgctgatg acaagtcagg aattgctgaa attatgacag
    10621 ccattgaata tctaactgct catcctgaaa ttaagcactg tgagattcgt gttggttttg
    10681 gtccagatga agaaatcggt gttggtgcca ataaatttga tgcagaagat tttgatgtgg
    10741 attttgccta cactgttgat ggtggtccac taggtgaact tcagtacgag actttctcag
    10801 ccgctggtgc tgaattgcat ttccaaggcc gtaatgtcca ccctggtact gccaaagggc
    10861 agatggtcaa tgcccttcag ctagcaattg attttcataa tcaacttcca gaaaatgacc
    10921 gacctgagtt aactgaaggt taccaaggtt tttaccatct aatggatgtg acaggtagtg
    10981 ttgaggaggc gcgtgcaagc tacatcattc gtgattttga aaaagatgcc tttgaagcgc
    11041 gtaaagcatc catgcaatct atcgctgata agatgaatga agaacttggg agcaaccgtg
    11101 tcactctcaa cttgacagac cagtactaca atatgaaaga agtcattgaa aaagatatga
    11161 ctccaattac cattgctaaa gccgttatgg aagatctagg tatcacgcct attatcgaac
    11221 caatccgggg tggaacagac ggctctaaga tttcctttat tggaatccca actccgaata
    11281 tctttgcagg tggcgaaaat atgcacggac gttttgaata cgttagcctt cagactatgg
    11341 aacgtgcagt tgataccatc attggcatcg tagcttataa aggctaaaaa gacgaggtag
    11401 ctcagctact tcgcctttct ttttattcta ctggtttttc ttgatttcca gtagttgtag
    11461 aagattctgt tgttttattt tctgaagttg attcagcagg tttagaatct cttgtattgc
    11521 ttggtttgtt ttcgtcgcta gcagtttcaa tgttagattc tgcagttgcg tttggttggt
    11581 tctcagcact ggtgttatca ctatttgctt cagcatttct tgctggactt gtttcttcac
    11641 ttgcgctagc ttttgactgg atttgatgat tcaaaactag aatagctttt gtcgattcaa
    11701 gtaaagctgt tttgtcttta ctcttagcag aaagttgatc taataatgca tccaccttat
    11761 caaagtccgc atcagatcca ttattacttt ctaaataaga gtgaagcgac atgagaatat
    11821 cgtagagttt ttgatagagt acaagtgtct gagaatcttg ctcagcattt tccttttctt
    11881 gttgaagggc gctagcgata cgagtcaaaa catcttttac ctgactgttt acttcatcca
    11941 agtctgcatc agccttgttt gtggcagctt ttagattttc tacttcttct gccaaggatt
    12001 gtctgattcc ttcttcatgg atttgttcca agagttgatt tgcattgttc aaaagacttt
    12061 ctacttcttc ctttctatct gtcgcagatt attggttgct atctaccatg tactcctaaa
    12121 acaggagagt tataatccaa gattacaagg ccttacagaa ataagaaatc cagataagac
    12181 aatgttcgtc caagacgcta ttcgcttcgc acagcagcac ggattcaata tactttaatt
    12241 ttaaagttta ggtgtcaaga cctcttttta gtgtgcccaa aatttagaga agtaatcaat
    12301 caactaactt ttattttttt caaactttca gtaaactgac ctaaagctaa ctcaatctgt
    12361 ctttgtagat gcttctgcta tcagctagaa gttgatctac ttttgccaag actgccttct
    12421 catcaaaagt tccaggttga tagttggatt gcagggatgg aatcttgttt ttcaaagccg
    12481 cttcatatcc cttagtttga accttgatgt agtgattgtg gtcgccatga ggaatcacaa
    12541 aaccttctga atcttcactt ataattcgat tggcatcaaa aaccatgacc atcttcttcc
    12601 tcatggtgga catgtagtga cggattactt aatacagaac tagaagaact tcctacctct
    12661 tccgtgttag agtgtgatgg gggattgtta agagatgact taggaatata gtgatagtgt
    12721 tccccatgtc ttactatata agcatcacct atatctctga caatatcatt agggttaaag
    12781 acatatgtgg ctgctaattc acctgccgac aagtcactct caggaatgaa atgatagtga
    12841 ccaccatgtg gtactatagt aggttgaaat agaatatgag caaattgata aggggatttt
    12901 aaagtaattt ctaacaatga tttagaaact atgatgtgct attctaaatt caactcacta
    12961 tatataacca tcatcggtag tataacgtcc ctgtaatttt gctacagata cttctgcact
    13021 agctccttta tcgtctttac catgttcttg tttttggcga ttgatttcat cttttgttcg
    13081 tacattttct gcatgagctt gatctttaag gtaaacataa tactttccat ctaccttaat
    13141 aatatatcct cccttaacct aactgacgat atcttgatct ttcggctgat agttgggggc
    13201 tttcattagt agctcttcac taaagagcgc atcaaaagga actttaccat tatagtagtg
    13261 ataatgatcg ccatgagaag ttacataacc ttgatctgta atcttaataa caatttgttt
    13321 tgcttgaatt ccttcttttt gactaaccta gtctggagtc aaattttcag tcttcttagt
    13381 gtctttatta ctgtttacat atgaaacacg atttttatct gtattggcct gttagctatg
    13441 ttggttcaga gcataaacac acagacttaa ggaaaggata acaacagatc cagctgctat
    13501 atatttcttt ttaaatttca taattacctc atttctataa ttatttatat gatgtcttca
    13561 ttattaaatg attaaataaa ttaattaacc agttaattaa cta
//
LOCUS       DAVGJL010000048        13343 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.048, whole genome shotgun sequence.
ACCESSION   DAVGJL010000048 DAVGJL010000000
VERSION     DAVGJL010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13343
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.048"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            211..1998
                     /locus_tag="VVE42_001130"
     CDS             211..1998
                     /locus_tag="VVE42_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEU3073331.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRVPPEDWYHSLYNLKAL
                     GFNTVETYVAWNLHEPREGEFHFEGDLDLEKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPAYIEAVGRYYDQLLPRLVPRLLDNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEECGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKGLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHSYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            1995..2471
                     /locus_tag="VVE42_001131"
     CDS             1995..2471
                     /locus_tag="VVE42_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEU3073332.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPVEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            2499..3404
                     /locus_tag="VVE42_001132"
     CDS             2499..3404
                     /locus_tag="VVE42_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEU3073333.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERFDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQSVVDFVEAYKWVADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVAVAAPSTPSESGEIEDDEF"
     gene            3391..4206
                     /locus_tag="VVE42_001133"
     CDS             3391..4206
                     /locus_tag="VVE42_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU3073334.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALALSALGHFALGM"
     gene            4213..4617
                     /locus_tag="VVE42_001134"
     CDS             4213..4617
                     /locus_tag="VVE42_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU3073335.1"
                     /translation="MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEE
                     FTAKFEAVIEGLDDFLVFADLLGGTPCNVVSRLIMEGRDIDLYAGMNLPMVIEFINAS
                     LTGADADYKSRAAESIVKVNDLLAGFDDDEDE"
     gene            4911..6077
                     /locus_tag="VVE42_001135"
     CDS             4911..6077
                     /locus_tag="VVE42_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEU3073336.1"
                     /translation="MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAA
                     FLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVANPAT
                     YLKKDVATVLVSFARSGNSPESVATVDLAKSLVDELYQVTITCAADGKLALQAHGDDR
                     NLLLLQPAVSNDAGFAMTSSFTSMMLTALLVFDPTEFAVKSERFEVVSSLARKVLDKA
                     EDVKELVDLDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INDNTVVLVFGTTTDYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVALGCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK"
     gene            6193..7230
                     /locus_tag="VVE42_001136"
     CDS             6193..7230
                     /locus_tag="VVE42_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEU3073337.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYEI
                     SYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSITPDGVPAKTPEAN
                     RDVVKHIYNGTLLKDIFAEEDEQIQLASGLDHPFALPVGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
     gene            7437..8291
                     /locus_tag="VVE42_001137"
     CDS             7437..8291
                     /locus_tag="VVE42_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU3073338.1"
                     /translation="MTYEYKSHIYLAETVLNVKDLASQTAFYQQVIGLEILSQTETES
                     ILGLGGKVLVQLIQAQESGEVREHYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEAIYLEDLEGNGIELYRDKPVSTWDIREDGRIIGVTEALAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSSQFYQKVLGLEDKFSVPSASWIAAGDYHHHLAVNEW
                     GGKGLASRKQGLPGLAYYVIEVAHKEELLTIAQRAQEVDAPIKWMTSSQLEITDSDGI
                     VTCIRLAR"
     gene            8441..8698
                     /locus_tag="VVE42_001138"
     CDS             8441..8698
                     /locus_tag="VVE42_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEU3073339.1"
                     /translation="MTNSKYITCLKRSEGQLCGIQKMIEGDRDCADIVTQLTAVRSSV
                     ERVIEMIITENLTECINQPLDDSEAQKERLEKAIRYLIKRK"
     gene            complement(9028..9120)
                     /locus_tag="VVE42_001139"
                     /pseudo
     CDS             complement(9028..9120)
                     /locus_tag="VVE42_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(9156..9452)
                     /locus_tag="VVE42_001140"
     CDS             complement(9156..9452)
                     /locus_tag="VVE42_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU3073340.1"
                     /translation="METSISMADFYGKYQNENLELIDVREAHEFQAGHAPGAKNLPLS
                     TLEQGYKELKPDHEYYVICQGGVRSASTCQFLSSQGLTVTNVEGGMNAWPGQVE"
     gene            complement(9442..9603)
                     /locus_tag="VVE42_001141"
     CDS             complement(9442..9603)
                     /locus_tag="VVE42_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(FAD)-dependent dehydrogenase"
                     /protein_id="HEU3073341.1"
                     /translation="MDKKQAYIVSCHSGLRNYIAESILKQAGFTVQNLDGAYSLYKMA
                     NPEGVEYGN"
     gene            9673..10437
                     /locus_tag="VVE42_001142"
     CDS             9673..10437
                     /locus_tag="VVE42_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEU3073342.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLGEEI
                     DRYAREVGANCVGEFVSATKTYPVYVINYKGEEVCLAQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPSRYMEMQPEAIAAIEEVLE
                     DRGIPYEEVMTWSTDGFYRETAEKVAYRKEEDCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL"
     gene            complement(10677..10829)
                     /locus_tag="VVE42_001143"
     CDS             complement(10677..10829)
                     /locus_tag="VVE42_001143"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073343.1"
                     /translation="MRTKASLRKLVTGYSKHCFEVVDRTDEVSSKHCFEVADRTDEVS
                     NIYTAR"
     gene            11047..12426
                     /locus_tag="VVE42_001144"
     CDS             11047..12426
                     /locus_tag="VVE42_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEU3073344.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIASRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLI
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
     gene            12440..13105
                     /locus_tag="VVE42_001145"
     CDS             12440..13105
                     /locus_tag="VVE42_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEU3073345.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQSWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
BASE COUNT         3664 a         2517 c         3052 g         4110 t
ORIGIN      
        1 ttctatacaa aattatttta ggtactatat tccaaattta aagagcgttt gaaatgggtc
       61 taaaaatgtt tgaaactaat tgaatgtgtg tataatagta aaaatgcagt aaatatcccg
      121 acaatttcta ttgacaattc aaacagattg gtttataatt aatataacaa caaatgaaat
      181 cgcaaacttt cgcggtcgga aggtagtttt atgacacgat ttgagatacg agatgatttc
      241 tatctcgatg gaaaatcatt taagatttta tctggtgcca ttcattattt tagggttcct
      301 ccagaggatt ggtatcattc gctctataac ttgaaggctc ttggttttaa tacggtagag
      361 acttatgttg cttggaattt acacgagcct cgtgaaggtg agtttcattt tgaaggtgat
      421 ctggatttag agaaatttct ccaaatagcg caggatttgg gtctctacgc aattgtgcgt
      481 ccgtctccat ttatctgtgc ggaatgggaa ttcggtggct taccagcttg gctcttgacc
      541 aagaacatgc gaattcgctc atccgaccca gcatatatcg aggcagttgg tcgctactat
      601 gatcagttat tgccaagact ggtgcctcgt ttgttggaca atggtggcaa tattctcatg
      661 atgcaggttg aaaatgagta tggttcttac ggagaagata aggcttatct gagagcgatt
      721 cgacagctaa tggaagagtg tggcgtaacc tgtcccctct ttacatcaga tggtccatgg
      781 cgagctactc tgaaagctgg aaccttaatt gaagaggacc tctttgtaac aggaaacttt
      841 ggttctaagg caccttacaa tttttcgcag atgcaggaat tctttgatga acatggtaag
      901 aaatggccac tcatgtgtat ggagttctgg gatggttggt ttaatcgttg gaaagaaccg
      961 attatcacac gggatcctaa ggaattggca gatgcagttc gagaagtttt ggaacaaggc
     1021 tccatcaatc tttacatgtt ccacggtggt acaaactttg gtttcatgaa tggttgctcg
     1081 gctcgaggaa ctttggacct gccacaagtt acgtcttatg attacgatgc ccttctggat
     1141 gaagaaggaa atccaactgc taaatatctt gcagtcaaga agatgatggc aacacatttt
     1201 tcagagtatc cgcagttgga accactctac aaagagagta tggagttgga tgctattcca
     1261 ctagttgaaa aagtttcttt gtttgaaacc ttagatagct tgtcaagtcc tgtagaaagt
     1321 ctctatcctc aaaagatgga ggagctggga caaagttatg gctacctact ttatcgaaca
     1381 gaaacaaact gggatgcaga agaagaaaga cttcgtatca ttgatggtcg agatagggcc
     1441 cagctgtatg tcgatggtca gtgggttaaa actcaatatc agacagagat tggggaagat
     1501 attttttatc aaggtaaaaa gaaagggcta tctaggttag atatcttgat agaaaatatg
     1561 gggcgtgtca actatgggca taagttctta gcggatacgc aacgtaaggg aattcggaca
     1621 ggggtctgta aggatctgca tttcttacta aactggaaac actatccact cccactagac
     1681 aatcctgaga aaattgattt ttcaaaagga tggactcaag gacaaccagc cttttacgct
     1741 tatgacttta cagtcgaaga gccaaaagat acttacctag acttgtctga gtttggtaag
     1801 ggggttgcct ttgtcaatgg gcagaatcta ggacgttttt ggaacgttgg cccaactctc
     1861 tcactttata tccctcatag ctatctcaag gaaggtgcca accgcatcat tatctttgaa
     1921 acagaaggtc aatataaaga agagattcat ttaactcgta aacctacact aaaacatata
     1981 aagggggaaa acttatgaca attgtaggat gccgtattga tggacgtttg atccacggac
     2041 aagtagccaa tctttgggct ggaaaactaa atgtttcacg cattatggtt gtagacgacg
     2101 aagttgtcaa caacgatatt gaaaagagtg gtttgaaact tgcgacacca ccaggtgtga
     2161 aattgagtat tttgccagtt gagaaagctg cagccaatat tcttgctggc aaatacgata
     2221 gccaacgtct ctttatcgtg gctcgtaaac cagaccgctt ccttggtttg gtagaagcag
     2281 gtgtaccact tgaaactctt aatgttggga atatgtctca aacaccagaa actcgttcta
     2341 ttacacgttc tatcaacgta gtagacaagg atgtggaaga cttccacaaa ctggcagaaa
     2401 aaggtgttaa acttactgct cagatggttc caaatgatcc aatttcagac tttttgagct
     2461 tattaaaata ggaaaaaaat ttttaggagg tcattgttat gatacaatgg tggcaaattt
     2521 tacttctcac tttgtactca gcttatcaaa tctgtgatga gttgacgatc gtttcatctg
     2581 caggttcccc tgtatttgct ggtttcatta ctggtttaat catgggagat gtgactactg
     2641 gtttacttat cggtggtaac ttgcaactgt tcgttcttgg ggttggtacc ttcggtggtg
     2701 cttctcgtat cgacgcaact tctggtgcgg ttcttgcgac agccttctct gtttcacaag
     2761 gaattgatgc accgcttgca attactacaa tcgctgtacc agtagcagct ctcttgactt
     2821 acttcgacgt tcttggtcgt atgactacta ccttctttgc tcaccgtgtg gatgctgcaa
     2881 tcgaacgctt tgactataaa ggtattgaac gcaactactt gcttggtgcg attccgtggg
     2941 ctctatctcg tgcccttcca gtcttctttg cccttgcttt tggtggtgcc tttgtacaat
     3001 cagtagtaga cttcgttgaa gcctacaaat gggttgcaga tggcttgaca ctcgcaggac
     3061 gtatgcttcc aggtcttgga tttgcaatct tgcttcgtta ccttccagtt aaacgtaacc
     3121 ttcactacct tgctatggga tttggtttga cagctatgtt gactgttctt tactcatatg
     3181 taacaggtct tggtggcgct gttgctggta tcgtaggtac tcttcctgct gaagttgctg
     3241 aaaaaattgg tttcgtgaac aacttcaaag gtttgtctat gattggtatt tctatcgtag
     3301 gtattttcct tgcagtgctt cacttcaaaa atagccaaaa agtagctgta gcagcacctt
     3361 ctacaccatc agaaagtggg gaaatcgaag atgacgaatt ctaattacaa acttacaaaa
     3421 gaagatttta atcaaatcaa caaacgtagc ttgtttactt tccaattagg ttggaactac
     3481 gaacgtatgc aagcttctgg ttacctttac atgatcttgc ctcagttgcg taaaatgtat
     3541 ggtgatggaa ctcctgaatt gaaagaaatg atgaaagttc atactcaatt cttcaatact
     3601 tcgccattct tccataccat tatcgctggt tttgaccttg ccatggaaga aaaagatggt
     3661 gtaggttcaa aagacgccgt taacggtatc aagacaggtt tgatgggacc attcgctcct
     3721 cttggggata caatctttgg ttcacttgta cctgctatca tggggtcagt cgcagcaact
     3781 atggctatcg ctggccaacc ttgggggatc ttcctttgga ttgcagttgc agtagcgtat
     3841 gacatcttcc gttggaaaca gttggaattt gcttacaaag aaggggttaa ccttatcaac
     3901 aacatgcaaa gtaccttgac agctttgatt gacgctgcat ctgtacttgg tgtcttcatg
     3961 atgggtgctc ttgtagcaac agtgattaac tttgaaattt cttacaagtt gccaatcggt
     4021 gaaaagatga ttgatttcca agacatcttg aaccaaatct tcccacgttt gcttccagca
     4081 atctttactg cctttatctt ctggttgctt ggtaagaaag gtatgaactc tactaaagct
     4141 atcggtatta ttatcgtact tgctttggct ctttctgccc ttggtcactt tgcacttgga
     4201 atgtaattcc ttatgactaa atcattaatt ttggtgagcc atggtcgctt ctgtgaggag
     4261 cttagaggta gcacagaaat gattatgggc ccacaagaca acatttacac agtagctctt
     4321 cttccagaag atggcccaga agaatttact gctaaatttg aagctgttat tgaagggttg
     4381 gatgatttcc tagtctttgc ggatcttctc ggtgggacac cttgtaatgt ggtgagtcgc
     4441 ttgatcatgg aaggtcgtga tattgacctt tacgcaggga tgaatcttcc aatggtgatt
     4501 gaatttatca atgcgagcct tacaggcgca gatgcggact acaagagccg tgctgcagaa
     4561 agcattgtga aagttaatga cctgttagcg ggcttcgatg atgacgaaga tgaataatac
     4621 tcttcgaaaa tctcttcaaa ctacgtcaac gtcgccttgc cgtaggtata tgttactgac
     4681 ttcgtcaatc ttatccggca acctcaaaac ggtgttttga gctgacttcg tcagttctat
     4741 ctgcaacctc aaagcagtgc tttgagcagc ctgcggctag tttcctattt tagttggaac
     4801 tcgattcaat tcatgtgaca acgtgaaaat cgttagagca ttttatatag aatatacatg
     4861 ggaatggagc ttactcccat tcccatattt aatagaaaaa gaggaactca atgctacatt
     4921 atacaaaaga agacttgctc gaattgggtg cagaaatcac tacgcgtgaa atctaccaac
     4981 aacctgatgt atggagagaa gcttttgaat tttatcaagc aaaacgtgaa gaaattgcag
     5041 ccttcctaca agaaatcgct gataaacatg actatattaa ggttatcttg acaggtgctg
     5101 ggacttctgc ttatgtggga gacaccttgc taccttactt taaggaagtc tatgacgaac
     5161 gcaaatggaa tttcaatgct attgcgacaa cagatatcgt tgccaatcca gcaacctatt
     5221 tgaaaaaaga tgtggcaact gtccttgtgt cttttgctcg tagtgggaac tcgcctgaaa
     5281 gtgtggcgac tgttgatttg gccaaatcct tggtggatga gctttatcaa gtgacgatta
     5341 cttgtgcagc agatggtaaa ttggctcttc aagctcacgg tgatgaccgt aatctcttgc
     5401 tcttgcaacc agctgtctct aatgatgctg gatttgccat gacttctagc tttacgtcta
     5461 tgatgttgac agctctcttg gtctttgatc ctacagaatt tgctgttaag tctgaacgtt
     5521 ttgaagttgt atctagtctt gcccgtaaag ttttagacaa ggcagaagat gtcaaagagc
     5581 ttgttgattt agactttaac cgtgtcatct atctaggcgc tggtcctttc tttggacttg
     5641 ctcatgaagc tcagctcaag attttggaat taactgctgg tcaagttgcg accatgtatg
     5701 aaagcccagt tggcttccgt cacggtccaa aatctcttat caacgacaat acagttgttt
     5761 tggtctttgg tacaacgaca gactacactc gtaagtacga cttggacttg gttcgtgaag
     5821 ttgctggtga ccagattgct cgtcgtgttg tgcttttgag tgatcaagct tttggtcttg
     5881 aaaatgtcaa agaagtggcc cttggttgtg gcggtgtctt gaatgatatt taccgtgtct
     5941 tcccttacat cgtttatgcc caactctttg ccctattgac ttcactcaag gtagaaaata
     6001 aaccagatac accgtctcct acaggtacag taaaccgtgt agtacaaggt gtcataattc
     6061 acgaatatca aaagtaagac agtgtttatg aattcttgac aagaggattt gtaaattatc
     6121 agataaacca tagattgtca gtacgctttc tatggtttgt ttgcttgaga gaaatagtaa
     6181 aaggagaaca gaatgaaagc atacacagag cgtgtatttg gaaatgttga gggcgaggat
     6241 gtcttggcct atcgatttga gacagacggt ggctaccaac ttgaggttat gacttatggc
     6301 gcgaccatct tgcgctatgt cgcacctgac aaggctggaa attttgccaa tgttatcttg
     6361 ggatttgatg actttgatag ttatgtaggc aatagtccca agcatggagc aagtgtaggt
     6421 cctgtagcgg gtcgtattgc aggtgcgacc tttgagctca atggtaagac ctatgacctt
     6481 gaggttaata atgctagcaa ctgtaatcac agtggttcaa ctggttggga ttccagcttg
     6541 tttgaagttg aagaagtaag cgatcatggc ttgactctct acacagagcg tacagatggg
     6601 acaggagggt tccctggaaa tctcaagatt tggatcagtt atcacttgga agaaactggt
     6661 gcctatgaaa ttagctacaa ggtaacgacc gatcaggata cgctggtcaa tccaaccaac
     6721 cacagctatt tcaacttgtc tggtgatttc acgcagacga ttgaccgtca tgtcttccaa
     6781 ctaaacacag agggcattta ctcaatcact cctgacggtg ttcctgccaa aactccagaa
     6841 gccaaccgtg atgtggtcaa acacatctac aatggtacct tgttgaagga tatctttgca
     6901 gaagaagatg agcaaatcca gctggcatca ggtttggatc acccatttgc ccttcctgta
     6961 ggccatgaca atgctggatt cctttatgac caaaattcag gtcgcttcct gcttttcaag
     7021 acagaagctc cttgctttgt ggtctacaca gcaaactttg tggatgaaag tgtcatcata
     7081 ggaggtcagc caatgctaca gcacaatggg attgctcttg aagcgcaagc tttaccagat
     7141 gccattcaca gtgaccttaa aggccaagtc attcttaaag ctggtcaaac cttcaccagt
     7201 aagacacgtt atgaacttgt tgtgaaataa aaaagctggt caaaaagtct ttaaaattaa
     7261 aataacgcat attgtcaggt gatgaaaagt ttgataatat gcgttttatt atgaaatgat
     7321 ttactttatc ttatcctgaa atggtgttat ttttgagtta tctggcttga ctttcttgat
     7381 ggaaattata taatagttgt aataacaatg attacaacga gaaaagagga ccatttatga
     7441 cctatgaata caagagtcac atttatttgg cagagacagt tttaaatgta aaggatttgg
     7501 ccagtcaaac agccttttat cagcaagtta ttggtttaga aatcttatct caaactgaga
     7561 cagagtccat tctaggcctt ggtggaaaag tcttggtaca gctgattcaa gcacaagaga
     7621 gcggagaagt gagggaacat tatggtcttt accatttggc cattctcttg cccacacgca
     7681 aggctttagc tgatgtcttg aaacacctga cagatttaca gattcctctt gtcggcggtg
     7741 cagaccatgg ttacagtgaa gctatttact tggaagattt ggagggaaat ggcattgaac
     7801 tctatcgaga taagccagtt tccacatggg atattcgaga agatggacgc attatcggag
     7861 tgacggaggc tcttgcggcg caggacattt atgagttggg ggaaagagta gagcccttta
     7921 tcctggcaga aggtacgaga atggggcata ttcatctttc cgtcaaggat agtcgaaagt
     7981 ccagccagtt ttatcaaaag gtgttagggc tagaggataa attcagtgtg cctagcgcta
     8041 gttggattgc ggctggagat taccatcatc atttagcagt taacgaatgg ggaggaaaag
     8101 gtctggcttc gcgtaagcaa ggcttaccag gtttagccta ctatgtcatc gaagtcgcac
     8161 ataaagaaga actgttaacg attgcccagc gagcacaaga agttgacgca ccaatcaaat
     8221 ggatgacatc gagccaattg gaaatcacag actcagatgg catcgtgacc tgtattcgtt
     8281 tagcaaggta aaattaggaa tttttaataa gttataagta ttgttagttt gctttttgaa
     8341 cggtctttgg taacagaact gcgtgagctg gatgggcgag aattttgaca aagaagctga
     8401 ttagagatat aatgaagaaa aatcgagcaa ggaaaaagaa atgacaaatt caaagtatat
     8461 tacttgtttg aaacgttcag agggccagtt gtgtgggatt caaaagatga ttgaaggaga
     8521 tcgtgactgt gctgatattg tgacgcaact gacagcagtg agatctagtg tagagcgcgt
     8581 gattgagatg ataattaccg aaaatcttac tgaatgtatc aatcagccac tagatgattc
     8641 tgaagctcaa aaggaacgcc tagaaaaggc tatccgatac ttgattaaac ggaaataaat
     8701 gaatgttttg agacgatatg tgcaaaggtc tattaactat tagaatagtt taatgtgagt
     8761 caaaagaagt tgtaaaaaat ctttaaaatt aaaaaaacgc atagtatcag gtgttgaatg
     8821 tactgacccc caaaagttag attttttctg tctaactttt ggggtgcagt tcataagaac
     8881 cttggtaata tgcgttttat tatgtagaga cttatactct tctaaaatct cttcaaacca
     8941 cgtcagcttc accttgccgt agatatggtt actgacttcg tcagttctat ccacaacctc
     9001 aaaacagtgt tttgagctga cttcgtcagt tccatccaca accttaaaac agtgttttga
     9061 gcagcctgcg gctaacttcc tagtttgcac tttaattttc attgagtatt acttcatctt
     9121 ctcatgaaat tgagtttttg cccagctcct cttgattatt ctacttgacc gggccaagca
     9181 ttcataccac cttctacgtt ggtaacggtg aggccttggg agctgagaaa ttggcaggta
     9241 gatgcagagc ggactcctcc ttgacaaatg acatagtatt catggtcagg tttgagttct
     9301 ttgtagcctt gctctaaggt acttaatgga agatttttgg cacctggtgc atgtcctgct
     9361 tggaattcat gtgcttcacg gacatcgata agttctagat tttcattttg gtattttcca
     9421 taaaagtcag ccatgctgat actagtttcc atattctact ccttctgggt tagccatttt
     9481 gtatagtgaa taagcgccgt caaggttttg gacggtaaat cctgcttgct tgaggataga
     9541 ctctgcgata tagttgcgca aaccactgtg gcagctgacg atgtaggctt gttttttgtc
     9601 cagttcatca gcttcctcct tgacactttg tcagtttttg atacaatagt acaaaattag
     9661 aggaggtggg ctatgattca gaaacatgcg attcccattt tagagtttga tgataatcct
     9721 caggcggtta tcatgcccaa tcacgagggg ttggatttgc agttgccaaa gaagtgtgtc
     9781 tatgcttttt taggtgagga gattgaccgc tatgcgaggg aagtaggggc gaactgtgtt
     9841 ggcgaatttg tttctgctac caagacctat ccagtctatg tcatcaacta caagggtgag
     9901 gaggtctgtc tggctcaggc tcctgttggc tccgctccag cagcccagtt tatggattgg
     9961 ttgattggtt atggtgtgga gcagattatc tctactggga cctgtggtgt cctagctgat
    10021 atagaggaaa atgcctttct agtccctgtt cgcgctttgc gagatgaggg agccagttac
    10081 cactatgtgg caccttctcg ttatatggaa atgcagccag aggctattgc tgctattgag
    10141 gaagttttgg aagacagagg gattccttat gaagaagtca tgacctggtc cacagatggt
    10201 ttttaccgag aaacggctga aaaggtggct tatcgcaagg aagaagactg tgctgttgtg
    10261 gagatggagt gttccgctct tgcggcagta gctcaattgc gtggggttct ctggggtgaa
    10321 ttgttgttca cagcagattc tctagcggac ttggaccagt acgacagtcg tgactggggc
    10381 tcggaagctt ttaataaggc gctagaactg agtttagcaa gtgttcacca cctttagttg
    10441 tactggcaaa ggatttgttt tatcataaaa tgtctagctc atacttttca aaaatatgtt
    10501 taaacgaggt caccttcctc ttgtcctagg catgttgagg ttgggaaaaa tctttaaaat
    10561 cagaaaaacg tatcatatca ggtgatgaaa actttgatac tatgcgtttt atgttggtaa
    10621 gatttagagt gagatgaaat gatactcttc gaaaatcttt tcaaaccagg tcagcttcac
    10681 cttgccgtat agatgttact gacttcgtca gttctatctg caacctcaaa acagtgtttt
    10741 gagctgactt cgtcagttct atccacaacc tcaaaacagt gttttgagta acctgtgact
    10801 agctttctaa gcgatgcctt ggttctcatt gcctataatc aaaaagagaa attttctcct
    10861 gaaaagcata tagagtagct ggcgttaaaa gctcctgtct cgcttttttg acctatagtc
    10921 acatctatca agtattgttc ttgcctaagc tatcaataaa aaggtggcat tttttaggct
    10981 tggtgttagt agattttgcc ttatcctatc taagtcattt cgaacttttt atggtacaat
    11041 ggaaacatgt tattcaaatt atctaaggaa aaaatagagc taggcttatc tcgtttatcg
    11101 ccagcccgtc gtattttttt gagttttgcc ttggtcattt tactaggctc tcttcttttg
    11161 agcttgccct ttgtccaagt tgaaagctca cgagcgactt attttgatca tcttttcact
    11221 gctgtctctg cagtctgtgt gacaggtctc tcaacccttc cagtagctca cacctataat
    11281 atatggggcc aaataatctg tttgctcttg attcagatcg gtggtctagg gctcatgacc
    11341 tttattgggg ttttctatat ccagagcaag caaaagctta gtcttcgtag ccgtgcaact
    11401 attcaggata gttttagtta tggagaaact cgatctttga gaaagtttgt ctattctatt
    11461 tttctcacga cctttttggt tgagagcttg ggagctattt tgcttagttt tcgccttatt
    11521 cctcaacttg gctggggacg tggtcttttt agttccattt ttctagcgat ctcagccttc
    11581 tgtaatgccg gttttgataa tttagggagc accagtttat ttgcttttca gaccgattta
    11641 ctggtcaatc tggtgattgc aggcttgatt attacaggcg gccttggttt tatggtctgg
    11701 tttgatttgg ctggtcatgt aggaagaaag aaaaaaggac gtctgcactt tcatacgaag
    11761 cttgtactat tgttgactat aggtttgttg ttatttggaa cggcaactac tctctttctt
    11821 gagtggaaca atgctggaac gattggcaat ctccctgttg ccgataaggt tttagttagc
    11881 ttttttcaaa cagtgacgat gcgaacagct ggcttttcta cgatagatta tactcaggct
    11941 catcctgtga ctcttttgat ttatatctta cagatgtttc taggtggggc acctggagga
    12001 acagctgggg gactcaagat tacgacattt tttgtcctct tggtctttgc acgaagtgag
    12061 cttctaggct tgcctcatgc caatgttgcg agacgaacga ttgcgtcgcg aacggttcaa
    12121 aaatccttta gtgtctttat tatctttttg atgagcttct tgataggatt gattctgcta
    12181 gggataacag ccaaaggcaa tcctcccttt atccacctca tatttgaaac catttcagct
    12241 cttagtacag ttggtgtaac ggcaaatctg actcctgacc ttgggaaatt ggctctcagt
    12301 gttatcatgc cacttatgtt tatgggacga attggtccct tgaccttgtt tgttagcttg
    12361 gcagattacc atccagaaaa gaaagatatg attcactata tgaaagcaga tattagtatt
    12421 ggttaagaaa ggaaagagca tgtcagatcg tacgattgga attttgggct tgggaatttt
    12481 tgggagcagt gtcctagctg ccctagccaa gcaggatatg aatattatcg ctattgatga
    12541 ccacgcagag cgcatcaatc agtttgagcc agttttggcg cgtggagtga ttggtgacat
    12601 cacagatgaa gaattattga gatcagcagg gattgatacc tgcgataccg ttgtagtcgc
    12661 gacaggtgaa aatctggagt cgagtgtgct tgcggttatg cactgtaaga gtttgggggt
    12721 accgactgtt attgctaagg tcaaaagtca gaccgctaag aaagtgctag aaaagattgg
    12781 agctgactcg gttatctcgc cagagtatga aatggggcag tctctagcac agaccattct
    12841 tttccataat agtgttgatg tctttcagtt ggataaaaat gtgtctatcg tggagatgaa
    12901 aattcctcag tcttgggcag gtcaaagtct gagtaaatta gacctccgtg gcaaatacaa
    12961 tctgaatatt ttgggtttcc gagagcagga aaattcccca ttggatgttg aatttggacc
    13021 agatgacctc ttgaaagcag atacctatat tttggcagtc atcaacaacc agtatttgga
    13081 taccctagta gcattgaatt cgtaaagagg gatgacccct cttttttgat gcccaagatg
    13141 gcaaatagag acagaagccc cttgtcttct agtaaaagtt cttcaaagac tggactttat
    13201 ggtaaaatag aaagaagtga caagagagag taatactcaa tgaaaatcaa agatcaaact
    13261 aggaaactag ctgcgggctg ctcaaaacac tgttttgagg ttgtggatag aactgacgaa
    13321 gtcagctcaa aacactgttt tga
//
LOCUS       DAVGJL010000049        13223 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.049, whole genome shotgun sequence.
ACCESSION   DAVGJL010000049 DAVGJL010000000
VERSION     DAVGJL010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13223
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.049"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            257..649
                     /locus_tag="VVE42_001146"
     CDS             257..649
                     /locus_tag="VVE42_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073346.1"
                     /translation="MKIKKLLKMVIPVLMISAVGTTFVEANQIGAFSNFVITTSYKRT
                     GYLTKENEGAEYIMNLNPCRNLHPMTVKHRIVNSNGEARSGESLTTCGTRSTHGNWAT
                     VGYVYAADMARQNWWDLSAAISGSWSPN"
     gene            671..1462
                     /locus_tag="VVE42_001147"
     CDS             671..1462
                     /locus_tag="VVE42_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073347.1"
                     /translation="MKIPLLTFARHKFVYVLLTLLFLALVYRDVLMTYFFFDIHAPDL
                     AKFDGQAIKNDLLKSALDFRILQFNLGFYQSFIIPIIIVLLGFQYIELKNKVLRLSIG
                     REVSYQGLKRKLTLQVASIPCLIYLVTVLIIAIITYFFGTFSPLGWNSLFSDGSGLQR
                     LLDGEIKSYLFFTCVLLIGIFINAIYFLQIVDYVGNVTRSAITYLMFLWLGSMLLYSA
                     LPYYMVPMTSLMQASYGDVSLMKLFTPYILYIVPYMVLEKYEDNV"
     gene            1455..2096
                     /locus_tag="VVE42_001148"
     CDS             1455..2096
                     /locus_tag="VVE42_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073348.1"
                     /translation="MFKNFNNIWLKRKIVLLLRIVLMMILINYLLSTAVQKQDAVIFF
                     KRELISIFSYNDYSEANLEIPKLLLNLSLFMVGWLSVILLESDLADHYHHLIRYQSSS
                     FFDYTRKRLVVISKFFTQDLLVWFLGLLPLGIHFKTVALFFLLAQLMILYLLLSYLIA
                     LISAGAGFSFFLYFLAFVGQEWMMDHIVTVYLLLLSLLVMLIVSRLEEKFKKG"
     gene            2100..2732
                     /locus_tag="VVE42_001149"
     CDS             2100..2732
                     /locus_tag="VVE42_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU3073349.1"
                     /translation="MRLEIINGQKIYGKRPILNQLNLVFQSGKIYGLKGDNGSGKTVL
                     LKILAGYIKLDKGKVLQDGKVYGIKNHYIQDAGILIEKVEFLSHLSLRENLELLRYFS
                     SKVTEKRIAYWIQYYDLQEFEDIEYRHLSLGTKQKMALIQAFISSPSILFLDEPMNAL
                     DEKSVRLTKQVILSYLKKENGLVILTSHISEDISDLCTDVLVVENGHIQM"
     gene            2760..3047
                     /locus_tag="VVE42_001150"
     CDS             2760..3047
                     /locus_tag="VVE42_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073350.1"
                     /translation="MAHLKSFITRYSKVYIGLVLLIWLSFFFIPWDRAILGVRIDIFI
                     IQKILLAFGILSILMALLSKKVSLFVFGLICCLSLWINLFITFAILPIFGN"
     gene            3193..5706
                     /gene="secA"
                     /locus_tag="VVE42_001151"
     CDS             3193..5706
                     /gene="secA"
                     /locus_tag="VVE42_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEU3073351.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMAALTDDQLKAK
                     TVEFKERYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYAQRYDVITADRDL
                     APEIQSMIKRTIERVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRALKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKRQ"
     gene            5787..6818
                     /locus_tag="VVE42_001152"
     CDS             5787..6818
                     /locus_tag="VVE42_001152"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU3073352.1"
                     /translation="MVFTAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            6820..7851
                     /locus_tag="VVE42_001153"
     CDS             6820..7851
                     /locus_tag="VVE42_001153"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU3073353.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEIFGCSITDPCLGWENTEALV
                     EEIYVTLTK"
     gene            7890..8258
                     /locus_tag="VVE42_001154"
     CDS             7890..8258
                     /locus_tag="VVE42_001154"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEU3073354.1"
                     /translation="MRMIVGHGIDIEELASIESAVTRHEGFAKRVLTTQEMERFTSLK
                     GRRQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLS
                     ISHTDQFVTASVILEENHES"
     gene            8248..9351
                     /locus_tag="VVE42_001155"
     CDS             8248..9351
                     /locus_tag="VVE42_001155"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEU3073355.1"
                     /translation="MKASPHRPTKALIHLGAIRQNIQQMGAHIPQGTLKLAVVKANAY
                     GHGAVAVAKAIQDDVDGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFREASEVEQAQDLLQQHGVCVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLDLPYDLIPALTLESALVHVKTVPAGACMGYGATYQADSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKPYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            9370..11385
                     /gene="recG"
                     /locus_tag="VVE42_001156"
     CDS             9370..11385
                     /gene="recG"
                     /locus_tag="VVE42_001156"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEU3073356.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     TKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLTGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKDLAEYKQALRRIKFEELFYFQMQ
                     LQTLKSENRVQGSGLVLDWSQEKVTAVKASLPFALTQAQEKSLQEILTDMKSDHHMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLVLLT
                     GSLKAAEKREVLETIAKGEADLIIGTHALIQDGVEYARLGLIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKPIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQVYVISPLIEESEALDLKNAIALSEELTAHFAGKAEVALLHGRMKSD
                     EKDQIMQDFKERKTDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMTETTNGFVLAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVASYISSIEAWQEDPEWRMIALHLEKKEHLD"
     gene            11701..12681
                     /locus_tag="VVE42_001157"
     CDS             11701..12681
                     /locus_tag="VVE42_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEU3073357.1"
                     /translation="MKNPALLEEIKTYRGRDEVPEDFDAFWDGEVKNVSTPPSYHLEE
                     RDFHIPQVKCYELTFEGSKEGKVYARIVLPKSEEKVPLIFHFHGYMGRGWDWADMLGF
                     TVAGYGVVSMDVRGQSGYSQDGLRSPLGNTVKGHIIRGAMEGRDHLFYKDVYLDIYQL
                     VEIVASLSQVDEKRLSSYGASQGGALALVAAALNPRIQKTVAIYPFLSDFRRVIEIGN
                     TSEAYDELFRYFKFHDPFHETEEEIMATLAYIDVKNLAHRIQGEVKMITGLDDDVCYP
                     ITQFAIYNRLTCDKTYRIMPEYAHEAMNVFVNDHVYNWLCGSEIPFKYLK"
BASE COUNT         3872 a         2447 c         2990 g         3914 t
ORIGIN      
        1 tatcaaaaag gagggaaaaa gtcatgggct ttaaaaaata tttgaagaat ttaccgaaaa
       61 actctggatt tttgatttgg agttggattc aacttatctg gtttgaaaca tggttttggg
      121 gataaagaaa ttagagtcta tacataataa gtaaaggagg aatttgtatg atataccatc
      181 gtttagaata agtctatatt taaaatagaa gttataagga atcttcttga ataaaaaatg
      241 aaaggaaaca gaaaaaatga aaattaagaa attattgaaa atggttattc ctgttttaat
      301 gataagtgct gttggaacta ctttcgtaga agcaaatcag ataggtgctt ttagtaattt
      361 tgttattact accagttata agagaacagg ttatttgact aaagaaaatg aaggtgcgga
      421 atatatcatg aacttaaatc cttgtagaaa tttacatccc atgactgtta agcatcgtat
      481 agtaaattct aatggagaag ctcgtagtgg agaatcgtta acaacctgtg gtactcgttc
      541 aactcatggt aattgggcaa cggttggcta tgtttatgca gcagatatgg caagacaaaa
      601 ttggtgggat ttatcagctg ccatttcagg tagttggtca ccaaattaat tcgtaaagta
      661 ggtataattt atgaagattc ctctcttaac ttttgcaagg cataaatttg tttatgtctt
      721 gcttactttg ctttttcttg ctttggttta tcgtgatgtt ttgatgactt atttcttttt
      781 tgatattcat gcgcccgatc tagctaaatt cgatggacaa gcaattaaaa atgacttatt
      841 aaaatcagca ttagattttc gtattctcca gttcaatcta ggtttttatc aatcatttat
      901 tattccaatc atcattgttt tgctaggttt tcaatatatt gagctgaaaa ataaagtttt
      961 acgattgagt attggaagag aagtgagtta tcaagggtta aaaagaaagt tgactttgca
     1021 agttgcaagt atcccttgtt tgatatattt agtgactgtg ctgataattg caattataac
     1081 ctatttcttt gggacttttt ctcctcttgg atggaattct ctattttctg atggaagtgg
     1141 tttacaaaga ctcctagatg gagagataaa aagctatttg ttctttactt gtgtcctact
     1201 aatcggtatt ttcatcaatg caatctattt tttacaaata gttgattatg tggggaatgt
     1261 gactcgttcg gcaatcacct atttgatgtt tctttggctt ggttctatgc tgctctatag
     1321 tgccttgcct tactatatgg ttcctatgac gagtttgatg caagctagct atggggatgt
     1381 aagtttgatg aaactcttta ctccttatat cctttatatt gtcccttata tggtgcttga
     1441 aaaatatgaa gataatgttt aagaatttta acaatatttg gctaaagaga aagatcgttt
     1501 tactacttcg tatagttctg atgatgattt tgataaacta tctattgtca acagcggttc
     1561 aaaagcagga tgctgttatc tttttcaaga gagaattgat ttcaatcttt tcctataatg
     1621 actattctga agcgaattta gaaatcccca aactcttgtt aaacctttcg cttttcatgg
     1681 taggatggct ctctgtcatt ttacttgaaa gtgatttggc agaccattac catcacttga
     1741 ttcgctatca atcaagctcc tttttcgatt atacaaggaa acgattggtt gtcatttcta
     1801 aattttttac tcaagattta cttgtctggt tccttggttt acttccgcta ggaattcatt
     1861 tcaaaacagt cgcacttttc tttttacttg ctcagttaat gattttgtac ttactactgt
     1921 cttatctgat agcactgatt agtgcgggcg ctggtttttc cttttttctc tattttttag
     1981 catttgtggg acaagaatgg atgatggatc atattgtaac agtgtattta ctactcttaa
     2041 gcttattagt tatgttgatt gttagtcgct tggaagagaa atttaagaaa ggataaacga
     2101 tgagacttga aattataaat ggacagaaaa tttatgggaa aagacctatt ttaaatcagt
     2161 tgaatttggt gtttcaatca ggaaaaattt atggacttaa aggtgataat ggatctggca
     2221 agacggttct tttaaagata cttgctggtt atattaagct tgacaaagga aaagttcttc
     2281 aagacgggaa agtctacggg ataaaaaatc attatattca ggatgcagga attttaattg
     2341 aaaaagtcga gtttttatct catttatccc tgagagaaaa tttggaactg ttaaggtatt
     2401 tttcatctaa agttacggaa aaaagaattg cctattggat tcaatactat gatttacagg
     2461 aatttgaaga cattgaatac cgtcatttat ccttaggaac aaagcaaaaa atggccttga
     2521 ttcaagcctt tatttcctct ccttctatac tctttctcga tgaacctatg aatgctttgg
     2581 atgagaagag tgtgaggtta accaaacagg tcattttatc ttacctgaaa aaagaaaatg
     2641 gtctggttat cctgacatcg cacatatcgg aagatatttc agacctttgt acagatgtat
     2701 tagttgtcga aaatggacat atacaaatgt aaaggatata caatcctagg agatggctta
     2761 tggcacatct aaaatcattt attacacgct attccaaggt ttatattggt ttagttctgc
     2821 tgatctggct gtctttcttc tttatccctt gggatagagc gattctgggg gtaaggattg
     2881 acatcttcat catacagaaa atcttgctag cttttggaat tctgtccatt ctcatggcct
     2941 tgctgtccaa gaaagtcagt ctctttgttt ttggactgat ttgctgtctt tctctttgga
     3001 ttaacttatt tatcacattt gccattttgc cgatttttgg caattaaaca gtcataaaag
     3061 tcggagaggt tagcttgaaa actaacctct ttttcctttt caaaatgggg attcttcctt
     3121 gaaaataatc agtaattgtg ctaaaattaa aggaacattc taaaatattc ggaatttaaa
     3181 gtaaggaaaa acatggctaa tattttaaaa acaattatcg aaaatgataa aggagaaatc
     3241 cgtcgtctgg aaaagatggc tgacaaggtt ttcaaatacg aagaccaaat ggctgctttg
     3301 actgacgacc aactaaaagc aaaaacagtt gaatttaagg aacgttatca aaatggagaa
     3361 tcactggatt cattgcttta cgaagcattt gcggttgtcc gtgaaggtgc caaacgtgtc
     3421 ctaggtctct tcccttataa ggttcaggtc atggggggga ttgttcttca ccatggtgac
     3481 gtgccagaga tgcgtacagg ggaagggaaa accttaactg cgaccatgcc ggtatacctc
     3541 aatgcccttt caggtaaagg ggttcacgta gttacggtta atgaatacct gtcagaacgt
     3601 gacgcgactg agatgggtga attgtactct tggcttggtt tgtcagtagg gattaacttg
     3661 gctaccaaat ctccaatgga gaaaaaagaa gcctatgagt gtgatattac ttactcaact
     3721 aactcagaaa tcggatttga ctaccttcgt gacaacatgg tcgttcgcgc tgaaaacatg
     3781 gtacaacgtc cgcttaacta tgccttggtc gatgaggttg actctatctt gattgacgag
     3841 gctcgtacac ctttgattgt atcaggtgcc aatgcggttg aaaccagtca gttgtatcac
     3901 atggcagacc actatgtaaa atctttgaac aaagatgact acatcatcga tgtgcagtct
     3961 aagactattg gtttgtctga ttcagggatt gacagggctg aaagctactt caaacttgaa
     4021 aatctctatg acatcgaaaa cgtggctttg actcacttta ttgataacgc ccttcgtgcc
     4081 aactacatca tgcttctcga tattgactat gtggtgagcg aagagcaaga aatcttgatt
     4141 gtcgaccaat ttacaggtcg taccatggaa ggtcgtcgtt attctgatgg attgcaccaa
     4201 gctattgaag ccaaagaagg tgtgccaatc caggatgaaa ccaagacatc tgcctcaatc
     4261 acgtaccaaa accttttccg tatgtacaag aaattgtctg gtatgacggg tacaggtaag
     4321 actgaggaag aagaatttcg tgaaatctac aacattcgtg ttattccaat cccaacaaac
     4381 cgtcctgttc aacgtattga ccactcagac cttctttatg caagtatcga atctaagttt
     4441 aaagcggttg tcgaagacgt taaggctcgt taccaaaagg gtcaacctgt cttggttggt
     4501 acagtagcgg ttgaaactag tgactacatt tctaagaaat tggttgcagc tggtgttcct
     4561 cacgaagtct tgaatgccaa aaaccactat agagaagccc aaatcatcat gaatgctggt
     4621 caacgtggtg ccgttaccat cgcaaccaac atggcgggtc gtggtaccga catcaagctt
     4681 ggtgaaggtg ttcgtgaact tggaggactt tgtgttattg gtacagaacg tcatgaaagt
     4741 cgtcgtatcg ataaccagct tcgtggacgt tcaggtcgtc aaggagatcc aggtgagtca
     4801 caattctacc tatctcttga agatgatttg atgaaacgtt ttggttctga acgcttgaag
     4861 ggaatctttg aacgtctcaa catgtctgaa gaggcgattg agtctcgcat gttgacgcgt
     4921 caggttgaag cagctcagaa acgtgtcgaa ggaaataact acgatacccg taaacaagtc
     4981 cttcaatacg atgatgtcat gcgtgaacaa cgtgagatta tctatgctca acgttacgat
     5041 gtcatcactg cagatcgtga cttggcacct gaaattcagt ctatgatcaa acgcacgatt
     5101 gaacgtgtcg ttgacggtca tgcgcgtgct aaacaagatg agaaactaga ggcaattttg
     5161 aacttcgcta agtacaactt gcttcctgaa gattctatta cgatggaaga cttgtcaggc
     5221 ttgtctgata aggccatcaa ggaagagctt ttccaacgtg ccttgaaggt ttacgatagt
     5281 caggtttcaa aactacgcga tgaagaagca gttaaagaat tccaaaaagt tttgattcta
     5341 cgagtggtgg ataacaagtg gacagatcat atcgatgccc ttgatcaatt gcgtaacgcg
     5401 gttggacttc gtggctatgc tcagaacaac cctgttgttg agtatcaggc agaaggtttc
     5461 cgtatgttta atgatatgat tggttcgatt gagtttgatg tgacacgctt gatgatgaaa
     5521 gcacaaattc atgaacaaga aagaccacag gcagaacgtc atatcagtac aacagcgact
     5581 cgcaatatcg ctgctcacca agcaagtatg ccagaagatt tggatttgag ccagattgga
     5641 cgcaatgaac tttgcccatg tggttctggt aagaaattta aaaactgtca cggtaaaaga
     5701 caataaaatg agatagttta gaggcggata tcttgtgaaa agtaaatttt tactgggtat
     5761 ccgtttgctt tataaggaga tgagttatgg tatttacagc aaaaagctct aaaataaata
     5821 tagaagaagt tcgtgccttg tcaaaattag aaggtcaggc tttggagagg aaatcacagc
     5881 gagatcaaga gctagaagcc attatacgtg gagaagacca gcgaattctc ttagtaatcg
     5941 ggccatgctc atctgacaac gaagaagctg tccttgaata cgctaagcgt ttggcagtcc
     6001 tacaagaaga agtggcagat cgtatcttta tggttatgcg tgtttatact gccaaacccc
     6061 gtaccaacgg agatggctat aagggcttga ttcaccagcc taacgcgaca gaagcgccta
     6121 gtcttatcaa tggaatcaaa gccgttcgcc atcttcacta tcgtgtcatc acagaaacag
     6181 ggatgacaac tgctgatgaa atgctttatc ctgaaaacct tccgcttgta gatgatttga
     6241 tttcttacat ggcagttggt gcccgttcag ttgaagacca gcaacaccgc tttgtggcaa
     6301 gtggggcagg attttctact ggttttaaaa atccaacctc tggaaatctc aatgtcatgt
     6361 ttaatgggat ttatgctgct caaaacaaac aaagtttcct tttcttagga aaagaagtag
     6421 aaacaactgg gaacccgctt tcacacgcta ttcttcgtgg tgctcttaat gagtatggaa
     6481 aaaatattcc caactactat tatgacaatt taattgatac cattgcccag tatgagaaaa
     6541 tgggcttgga aaatcctttt atcatcattg ataccaatca tgacaattct ggtaagcagt
     6601 atattgaaca gatccgaatt gtccgccaga ccttgattaa ccgtgcttgg aatgaaaaaa
     6661 ttaagcagtt cgttcgtggt tttatgattg agtcttatct ggaagatggt cgacaaaatg
     6721 agccagaagt atttggtaag tctatcacag acccttgcct gggttgggat aacacagaag
     6781 ctcttgtcag agaaatttac aaaacgttag gagaataaga tggcatttat tgaaaaaggt
     6841 caagaaatcg atatggaagt catcaaggct gaaacccaat tgtctgcgga agccttgaga
     6901 ctcaaggaaa gccgtgacag ggaattggca gatattattt caggggaaga tgaccgtatt
     6961 ctcttggtga ttggtccttg ctcttctgat aatgaagagg cggtcttgga atatgctcgc
     7021 cgtttatccg ccttgcaaaa gaaggtagcg gacaagattt tcatggtcat gcgcgtgtat
     7081 actgctaagc ctcgtaccaa tggagacggc tataaaggat tggttcacca gccagatact
     7141 tctaaggctc caagcctgat taacggcttg caggctgtgc gccagttgca ctaccgcgtg
     7201 attacagaga ctggtttgac aacggcagat gagatgcttt atccgtcaaa tctgatcttg
     7261 gtggatgact tggtcagcta ccatgccgtt ggagctcgtt ctgtggaaga ccaagagcac
     7321 cgctttgtgg cttctgggat tgatgcacca gtagggatga aaaatccaac ctcaggaaat
     7381 ttgggtgtta tgtttaacgc catctatgct gctcaaaaca agcaaacctt cctttatcat
     7441 gggcaggaag ttgagacatc aggtaatcct ttggcccatg ttatcctccg tggagcagtc
     7501 aacgagtatg gcaattatat gccgaattac tactatgaaa acctactcca agccattgaa
     7561 cgctatgaaa ccatgggact tgaaaatcct tttatcctca ttgacaccaa ccatgataac
     7621 tcaggcaagc aatatatgga gcagattcga attgttcgcc agaccttgca gaatcgtgat
     7681 tggaatgaga aaattaaaaa gacggttcga ggatttatga ttgaatctta cctagcagat
     7741 ggtcgtcaaa accaaccaga gatctttggt tgctctatta ctgacccttg cctaggttgg
     7801 gaaaatacag aggccttggt agaagagatt tatgttacct tgacaaaata agtgaaaagg
     7861 atggagttgg ggaatctcaa ctccttttga tgagaatgat agttggacac ggaattgaca
     7921 tcgaagaatt ggcttcgata gaaagcgcag ttacacgaca tgaaggattt gctaagcgtg
     7981 tactgaccac tcaggaaatg gagcgcttca ccagtctcaa aggacgcagg caaatagaat
     8041 atttagctgg tcgctggtcg gctaaggagg ctttttccaa ggctatggga acgggcatta
     8101 gcaagctggg ttttcaggat ttggaagtct tgaacaatga acgtggggcg ccttatttta
     8161 gtcaggcacc attttcagga aagatttggc tgtctatcag ccacaccgat cagtttgtga
     8221 cagccagtgt cattttggag gaaaatcatg aaagctagtc cacatagacc aaccaaggct
     8281 ctgattcatc tgggagctat tcgacaaaat attcagcaaa tgggggctca tatccctcaa
     8341 ggaacgctca agttggctgt ggtcaaggcc aatgcttatg gccatggagc tgttgccgtt
     8401 gccaaggcaa ttcaagatga tgttgatggc ttttgcgttt ccaatatcga tgaagccatt
     8461 gaactcagac aagctggact tagcaagcca atcctaattt taggagtttc tgaaatcgaa
     8521 gctgttgctc tagctaaaga atatgacttc accttgacag tggctggact ggagtggatt
     8581 caagcactct tagataagga agtggaccta actggattga cagtccacct caagattgat
     8641 tcagggatgg gacggattgg ttttagagag gcaagtgagg ttgagcaggc tcaagatttg
     8701 ctccaacaac acggtgtttg tgttgaagga atctttaccc actttgctac tgctgatgag
     8761 gaatcagatg actattttaa tgcccagtta gaacggttta aaactatttt agctagtatg
     8821 aaggaagttc cagagctggt tcatgctagc aattctgcaa cgactctttg gcatgtagag
     8881 actattttca atgcggttcg tatgggagat gccatgtatg gtctcaatcc aagtggagcg
     8941 gtcttggatt tgccttatga tttgataccg gccttgacct tggagtctgc tctggttcat
     9001 gtcaagacag ttccagctgg agcttgcatg ggctatggag caacttatca agcggatagc
     9061 gagcaagtca tcgcgaccgt gccaatcggg tatgcagatg gatggacaag agacatgcaa
     9121 aatttctctg tcttggtaga tggccaagct tgcccaattg tcggcagggt ttcgatggac
     9181 caaatcacta ttcgattgcc taagccttac ccactcggaa ccaaggtaac cttgattggc
     9241 tccaatgggg ataaggaaat cactgcaact caggtagcga cctaccgcgt aaccattaac
     9301 tatgaggtgg tttgcctcct cagcgaccgt attccgagag aatattatta gaaaagaaag
     9361 gagtggagca tgaatctaca tcaacccttg catgtcttgc ctggtgtggg accaaagtca
     9421 gcagaaaaat acgccaaact aggaattgaa aacttgcaag atctcttgct ctactttcct
     9481 ttccgttatg aagacttcaa aaccaagcag gtgctggagc tggaagacgg tgagaaggca
     9541 gttctttctg gtcaggtagt gactcctgct agtgtccagt attatggttt caagcgcaat
     9601 cgcctgcgtt ttagtctcaa gcagggagag gtcgtttttg cggtgaattt ctttaaccag
     9661 ccctatctgg ctgataaaat agagttggga gcaacccttg ctgtctttgg aaaatgggac
     9721 cgcgctaagg ctagtctgac tgggatgaag gttctggctc aggtagaaga tgacctccag
     9781 cctgtctatc gtctggctca gggaatcagt caggccagtc tggtcaaggt catcaagacg
     9841 gcttttgatc agggactgga cctcttgata gaagaaaatc tgccccagtc tttactagac
     9901 aaatacaaac tcatgtcccg ttgtcaggca gtccgagcta tgcattttcc aaaggatttg
     9961 gcagaataca agcaggctct tcgccgtatc aagtttgagg aactctttta tttccaaatg
    10021 cagctgcaga cgctcaagtc tgaaaataga gttcagggaa gtggtctggt tctggattgg
    10081 tctcaggaaa aagtgacagc agttaaagca agtcttcctt ttgccctgac ccaagctcag
    10141 gaaaagagtt tgcaggaaat tttaactgat atgaagtccg accaccacat gaatcgtctc
    10201 ctacaaggag atgtggggag cggaaaaacg gtagtcgctg gcttggccat gtttgcggca
    10261 gtgacagcag gttatcaggc tgccctaatg gtaccaacag aaatcctcgc agagcaacac
    10321 tttgagagtt tacagaacct ttttcccaat ttgaaactgg ttctcttgac aggttccttg
    10381 aaagctgcag aaaagagaga ggtcttggag accattgcca agggtgaggc tgatttgatt
    10441 ataggaactc acgctctgat acaagatggg gtggagtatg ctcgtcttgg tttgattatt
    10501 atcgatgagc agcaccgttt tggtgtaggg caaaggcgta ttttacggga aaaaggcgac
    10561 aatccagatg tcctcatgat gacggcgact cccattccac ggacgcttgc catcacagcc
    10621 tttggagata tggatgtttc cattatcgac cagatgccag caggtcggaa gcctattgtg
    10681 acgcgctgga tcaaacatga gcaactacct caggtcttga cttggttaga gggggaaatt
    10741 caaaaaggtt ctcaagtcta tgtcatctct cctttgattg aagaatcaga agctctagat
    10801 ttgaaaaatg ccattgcctt atcagaagag ctgacggctc attttgcagg caaggcagag
    10861 gtggctcttc tacatggtag gatgaagagt gacgaaaaag accagatcat gcaggatttc
    10921 aaggagagaa agacggatat tctggtttcg acgacggtta ttgaggttgg ggtcaacgtt
    10981 cccaatgcga ctgtcatgat tatcatggat gccgatcgct tcgggctcag ccagcttcac
    11041 cagcttagag gtcgtgtcgg tcggggggac aagcagtcct acgctgttct cgttgccaat
    11101 cccaagacgg attctgggaa agaccgcatg cgcatcatga cagaaacgac caatggattt
    11161 gtccttgcgg aggaagattt gaaaatgcgt ggttctggtg agatttttgg aaccagacag
    11221 tcaggacttc cagagttcca agtggctgat attatcgaag attttccgat tttagaagaa
    11281 gcaagaaagg ttgctagcta cattagttct atagaagctt ggcaagaaga tccagagtgg
    11341 cgcatgattg cccttcatct ggaaaagaaa gaacatctgg attaagcttt ctctaaggaa
    11401 aacttaagct agtttttagg tttggctctt atactaaagt catcaaaaag aaacgaggac
    11461 tctcatatga cagtaacgat taaagtaaat taccaaacca ctttccaaaa gaaggaagca
    11521 aaaaactagt ataaacagaa gagagagcga aatgctcttt ttcgtttcta aaactacttt
    11581 cagcccatca tcctaaaagt aaagaatcta aattcacttt ctatttatcc ttctttcttg
    11641 cattgattac atagatatgc tacagttgtg gtaacgatta caaaataaaa ggagcatgct
    11701 atgaaaaatc cagctttgct agaagaaatt aagacctata gaggaaggga tgaggttccg
    11761 gaagactttg atgctttctg ggatggggaa gtgaaaaatg tttccacacc tccatcctac
    11821 cacttggagg aaagagattt ccacattcct caagtcaagt gctatgagtt aacatttgaa
    11881 ggaagcaagg aaggaaaggt ctatgcacgc attgttcttc caaagagtga ggagaaggtt
    11941 ccactaatct tccattttca tggttatatg ggacgtggct gggactgggc cgacatgctg
    12001 ggcttcaccg tagctggtta tggtgttgtt tccatggatg tgcggggcca gtcaggttac
    12061 tcacaagacg gcttgcgttc tcctttagga aataccgtga aggggcatat tatccgtggt
    12121 gctatggaag gtcgggacca cctcttttat aaggatgttt atctggatat ttaccagttg
    12181 gtcgaaattg ttgctagtct gtctcaggtt gatgagaagc gtctttctag ctatggtgcc
    12241 tcacaaggag gggctctagc tctagttgca gcagcgctca atcctcgaat tcagaaaaca
    12301 gttgccattt atcccttctt gtcagacttc agacgggtga ttgagattgg taatactagc
    12361 gaggcttacg acgaactttt ccgttatttc aagtttcacg accccttcca tgaaacagag
    12421 gaggaaatca tggcgaccct tgcctatatc gatgtcaaaa atcttgccca tcgtatccaa
    12481 ggtgaggtta agatgattac gggcttggac gacgatgttt gctatcccat tacccagttt
    12541 gcgatttata atcgtctgac ctgcgataaa acctatcgta tcatgcctga gtatgctcac
    12601 gaagccatga atgtatttgt taatgaccac gtctacaact ggctctgtgg aagtgagatt
    12661 ccttttaaat atctaaaata aggagtcgac tctaagcaca aaatcttaaa aattacaaac
    12721 acgcatagta tcaggggatt aaaaaaactt gatactatgc gttttatcat ggaaatatag
    12781 taaaatgaaa caagaatagg acaaaacgat caggacagtc aaatcgattt ctaacaatgt
    12841 tttagaagca aatgtgtact attctagttt caatctatta tatttataga attttttgtt
    12901 gctagatttg tcaaattgct taaaataatt tttttcagaa agcaaaagcc gatacctatc
    12961 gagtagggta gttcttgcta tcgtcaggct tgtctgtagg tgttaatact tttcaaaaat
    13021 ctctttaaac cacgtcagct tcgccttgcc gtatatatgt tactgacttc gtcagttcta
    13081 tctgccacct caaaacggtg ttttgagctg actttgtcag tcttatctac aacctcaaaa
    13141 cagtgttttg agcatcatgc ggctagcttc ttagtttgct ctttgatttt cattgagtat
    13201 aaaaacagat gagtttctgt ttt
//
LOCUS       DAVGJL010000050        12985 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.050, whole genome shotgun sequence.
ACCESSION   DAVGJL010000050 DAVGJL010000000
VERSION     DAVGJL010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12985
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.050"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            90..524
                     /locus_tag="VVE42_001158"
     CDS             90..524
                     /locus_tag="VVE42_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU3073358.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQENVEAIDFVEGMSADELK
                     QKILLAISNEEEVLILSDLLGGSPFKVSSTIMGENPAKTMNVLSGLNLAMLMEAVFAR
                     MAHSFDEVVNKSVVAAQGGVVNGKELFSTDAEEEEEDFESGI"
     gene            536..1726
                     /locus_tag="VVE42_001159"
     CDS             536..1726
                     /locus_tag="VVE42_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEU3073359.1"
                     /translation="MIKKVTIEKIKSPERFLEVPLLTKEEVGQAIDKVIRQLELNLDY
                     FKEDFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYEYSQQDAFKNIAHKNVLSFLDR
                     VNKRVELDHHDLGFLYTPSCMAEYKINGDGEAREATLKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYASANNVIRDDASSFHT
                     FYFDPETGQPFKGVTRQGYSDDSCWARGQSWGVYGIPLTYRHLKDESCFDLFKGVTNY
                     FLNRLPKDHVSYWDLIFNDGSAQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEHYANDQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            1737..2228
                     /locus_tag="VVE42_001160"
     CDS             1737..2228
                     /locus_tag="VVE42_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEU3073360.1"
                     /translation="MTMPNIIMTRIDERLIHGQGQLWVKYLGCNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKDVKDALTLVEGGVPIKE
                     INIGNIHNATGKEQVTRSIFLGEEDKAALKELSQTHQVTFNTKTTPTGNDGAVQVNIM
                     DYI"
     gene            2243..3022
                     /locus_tag="VVE42_001161"
     CDS             2243..3022
                     /locus_tag="VVE42_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEU3073361.1"
                     /translation="MSINVFQAILIGLWTAFCFSGMLLGIYTNRCIVLSFGVGIILGD
                     LPTALAMGAIGELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVSPEAALALST
                     PIAVAIQFLQTFAYTVRAGAPETAMKHLKNHNLKKFKFTLNATIWLFAFIGFTLGCLG
                     ALSMDTLLKLVDYIPPVLLTGLTVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLNIPTIGIAIVGTIFALIEFYNKPKTADHVVEEEAHDDWI"
     gene            3009..3827
                     /locus_tag="VVE42_001162"
     CDS             3009..3827
                     /locus_tag="VVE42_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU3073362.1"
                     /translation="MTGSNKLTKRDYLKTSLRAFFCQNGFNYSNYQGLGYANVMYPAL
                     KKHYGEDQEGFYQALEENCEFYNTNPHFLPFITSLHLIMLENGRPAKETRSIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASFAQEGLVVGPILFFLAMNTILTAIKLSTGLYGYK
                     LGTTVIDKLSEQMATISRIANIIGVTVIAGLAATSVKIMVPITFAAGEVKADAKQSIV
                     SIQGMLDKVAPALLPALFTLLVYYLIKEKKWTTYKLVILTVIIGIIGSWLKIIA"
     gene            3827..4120
                     /locus_tag="VVE42_001163"
     CDS             3827..4120
                     /locus_tag="VVE42_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEU3073363.1"
                     /translation="MDTTLFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQMEQRQEYLA
                     SLTSGDEVLLLSGIHGKIISIKDDLVSLQIAKGVVIYVEKESVMGKTKELLFK"
     gene            4142..6043
                     /locus_tag="VVE42_001164"
     CDS             4142..6043
                     /locus_tag="VVE42_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEU3073364.1"
                     /translation="MKEERRQFFERVDGNQCRDYILSHCSKDYEKVKSSLERLMDNRF
                     MFDSPWDMEPCSKIHQIQPMVWDQVFEDDPEWSYMLNRQEYLLQFMIGYLVEGDKDYI
                     QKCKFFLFDWIEQVREFSPQSLMTRTLDTGIRSFTWLKLLLLLLKFDLLEEKELEKIL
                     VSLEKQIDFMKSHYRAKYTLSNWGILQTIPMLAIYHFFSDKMDLEEAYHFASEELKQQ
                     IETQILGDGSQFEQSILYHVEVYKALLDLCLLLPDLQDSYQELLEKMATYIQMMTGLD
                     GRTLAFGDSDSTETTEILSLSAVVLNKEDLLNGLDVKVDLLSLLFLGREKVKRLQEFE
                     KRAWQPKSMIFEDSGHVCIKDEHRYLFFKNGPLGSAHSHSDENSFCLQYQGQPIFIDA
                     GRYSYREIYERYLLKSAWSHSTCIVDGKAPERITGSWEYEYYPHSLFCHHKEREGMHY
                     IEGAYWSAEPDLPYLHRRKILMLVEDVWLLVDDIRCQGQHEALTQFILDKDVTYQDGK
                     INQLRLWSEVDFDLEDTIISPKYNELERSSKLTKRQFFENQMLDYTIIAHESFEIIRH
                     SVYQTDDREVENALAFEVKNDETDKLILLLSEDICVGEKLCLVDGTKMRGKCLVYDKI
                     NERMIRLQC"
     gene            6103..7104
                     /locus_tag="VVE42_001165"
     CDS             6103..7104
                     /locus_tag="VVE42_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU3073365.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQIVKGIEDIASQNGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANMRHASLT
                     IEDKHTNLEQIKEFLQKEIDPDEKTLVFIPNCWALPLVFTVIKELNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPAFEEGQQATKILIDQIEGLNQEERQQVLDCSVSWKESTF"
     gene            complement(7375..7953)
                     /locus_tag="VVE42_001166"
     CDS             complement(7375..7953)
                     /locus_tag="VVE42_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HEU3073366.1"
                     /translation="MKKEDFTTRLLRNLFHIQGPFDECRQEIIYKACARSMVQIVYSS
                     FFLFLFYLLFGRFIESVRYAMPYVYSGLIFFITLKTQRVVKELHLEKDDKSEIILKTY
                     SKAQIKFRSWVVFIGLQIGLFTLLIFHKVFVQQMSLSDFGKLLMQFDKSVPFLMYGMI
                     IGSIFGTLTYGFLSLQEEKTPKNTKQKEKSKQ"
     gene            complement(7980..8306)
                     /locus_tag="VVE42_001167"
     CDS             complement(7980..8306)
                     /locus_tag="VVE42_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073367.1"
                     /translation="MKSLLTLLTYHLLFSSLLIFIIVSGNLPFSEIFLLLVFIPALNK
                     GLTYLKIDSQKTRILNLALCFMILSFPQLMLISSGWKYYLLLTIASLSSITYLYYLYQ
                     FVKENQ"
     gene            complement(8316..8558)
                     /locus_tag="VVE42_001168"
     CDS             complement(8316..8558)
                     /locus_tag="VVE42_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073368.1"
                     /translation="MKKEKKQLRHPGLKAGSIYGIVIFFIIPLIDTLTSENPNFITSL
                     LNTKHIFKTILGAFFFGVMMHIVDSLRIARAKKDQD"
     gene            complement(8569..9111)
                     /locus_tag="VVE42_001169"
     CDS             complement(8569..9111)
                     /locus_tag="VVE42_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HEU3073369.1"
                     /translation="MKKETFTEKLIKRTYGISDPLDEYKRREADRIGNQVFIFLFYLM
                     IFGNLIPLVLAYKYPQLVALVYPPLILVIALIVAGYLTYQMQKTGITTIEPDMLSEKE
                     SKQLHYPGLKAGIVYGLMSFFSTPLLLILLGESQDYLQSLLAFKNIFSSILQSVFFGV
                     VIQILISRRIAKAKKNQDND"
     gene            complement(9127..9321)
                     /locus_tag="VVE42_001170"
     CDS             complement(9127..9321)
                     /locus_tag="VVE42_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU3073370.1"
                     /translation="MNRVKEFRKELGISQLELAKDIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWEDNF"
     gene            9488..10438
                     /gene="rsmH"
                     /locus_tag="VVE42_001171"
     CDS             9488..10438
                     /gene="rsmH"
                     /locus_tag="VVE42_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEU3073371.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNHYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQAREVKPIETTTELAEIIKSAKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMEMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLKPKMELVSRKPILPSAEELEANNRSHSAKLRVARKIHK"
     gene            10448..10765
                     /gene="ftsL"
                     /locus_tag="VVE42_001172"
     CDS             10448..10765
                     /gene="ftsL"
                     /locus_tag="VVE42_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEU3073372.1"
                     /translation="MVDKKETTSQFLQSRIKKFSRVEKAFYLSIAFTALVLAVSIIFM
                     QTRLLQVQSDLTKINAQIEEKKTELDDAKQEVNELIRSERLKEIANSKDLKLNNENIR
                     SAE"
     gene            10769..>12985
                     /locus_tag="VVE42_001173"
     CDS             10769..>12985
                     /locus_tag="VVE42_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEU3073373.1"
                     /translation="MKWTKRITRFAIRNRKSPAENRKIVGKYISLLAVVLFAVFLVNF
                     AVIIGSGSKFGTDLVKEAKKVHQITRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDK
                     KYKSATGKILYVEDAQFNKVAEVFHKYLDMDEAYVKEQLSQPNLTQVSFGAKGNGITY
                     ANMMAIKKDLKDASVEGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     MESSLNSILAGKDGIITYEKDRLGNIVPGTEQVSQQTVDGKDVYTTLSSPLQSFMETQ
                     MDAFLEKVKGKYMTATLVSAKTGEILATTQRPTFNADTKEGITEDFVWRDILYQSNYE
                     PGSAMKVMTLASSIDNNTFPSGEYFNSSEFKIADATIRDWDVNEGLTTGGMMTFLQGF
                     AHSSNVGMSLLEQKMGDATWLDYLKRFKFGVPTRFGLTDEYAGQLPADNIVSIAQSSF
                     GQGISVTQTQMLRAFTAIANDGVMLEPKFISAIYDTNNQSVRKSQKEIVGNPVSKEAA
                     STTRNHMILVGTDPLYGTMYNHYTGKPIITVPGQNVAVKSGTAQIADEKNGGYLVGST
                     NYIFSVVTMNPAENPDFILYVTVQQPEHYSGIQLGEFATPILERASAMKESLNLQSPA
                     KNLDKVTTESSYAMPSIKDISPGELAEALRRNIVQPIVVGTGTKIKETSVEEGTNLAP
                     NQQVLLLSDKVEEIPDMYGWKKETAETFAKWLDIELEFEGSGSVVQKQDVRTNTAIK"
BASE COUNT         4035 a         2255 c         2907 g         3788 t
ORIGIN      
        1 tttaaaaaaa ctattgacaa gataaaaata aaggattaca atggggaata taaagtaaac
       61 cggtaaacct aaacaagaaa ggagaaaaga tgaaaattgt acttgtaggg catggacatt
      121 ttgctacagg gatttatagt tctttacaat tgattgcagg caatcaagaa aatgtggagg
      181 caattgactt tgtggaagga atgtcagcag atgaactcaa gcaaaaaatc ttacttgcaa
      241 tttcaaatga agaagaagtt ttaatcctaa gtgatctctt gggaggatcg ccattcaagg
      301 tttcttctac cataatggga gaaaatccag ccaaaacaat gaatgttctt tcgggtttga
      361 acttagccat gttaatggaa gcagtctttg ctagaatggc tcatagcttt gatgaggttg
      421 ttaataaatc agtagtggcg gcccagggcg gagtcgtaaa tggtaaagaa ttgttttcaa
      481 cggatgcaga ggaagaggaa gaagatttcg aatcgggtat ttaaagggta aaagaatgat
      541 aaaaaaggtt acgattgaaa aaataaaatc gcctgagcgc ttcttagaag taccacttct
      601 gacgaaagaa gaagtcggcc aggcaatcga taaggttatt cggcagttag aactcaacct
      661 tgactatttc aaggaagatt tcccgacgcc agctaccttt gataatgtct atccaatcat
      721 ggataacacg gaatggacca atggtttctg gacaggagaa ctgtggttgg cttatgaata
      781 cagtcaacag gatgcattta aaaacatcgc tcataaaaat gttctttctt tcctggatcg
      841 tgtcaataag agagtagaat tggatcacca tgatctcggc ttcttgtaca caccgtcttg
      901 tatggctgaa tataagataa atggagatgg agaggctaga gaagcaacct tgaaagctgc
      961 agataagttg attgaacgct atcaagaaaa aggtggtttt attcaagctt ggggagactt
     1021 gggcaagaaa gagcattacc gtttgattat cgactgcttg ctcaatatcc aactcttatt
     1081 ctttgcttat caagaaacag gcgatcaaaa atactacgat attgcagaaa gtcatttcta
     1141 tgcttcagct aataatgtaa tccgtgatga cgcttcgtcc ttccacacct tctattttga
     1201 tcctgagaca ggtcaaccct ttaaaggtgt aacgagacaa gggtatagtg atgattcatg
     1261 ctgggcacgt ggtcaatcat ggggagtcta tggtattcct ttgacttatc gtcacttaaa
     1321 agacgagtcc tgctttgact tgtttaaggg tgtgaccaat tatttcttga atcgtctgcc
     1381 aaaagatcat gtgtcctatt gggatttgat ttttaatgat ggtagtgctc aatcacgaga
     1441 ttcttcagca acagctatcg ccgtctgtgg gattcatgaa atgctaaaac atctcccaga
     1501 ggtggatgct gacaaagata tttataaaca tgctatgcat gccatgcttc gttccttgat
     1561 cgaacattat gcaaatgatc aatttacccc tggtgggaca agtctcctcc acggtgtgta
     1621 ctcatggcat tcaggtaaag gagtggatga aggcaatatc tggggtgact actattacct
     1681 agaagccctt atccgtttct acaaagactg gaacctatat tggtaggagg agaaatatga
     1741 caatgccaaa tattattatg acccgtatcg atgaacggtt gattcatgga caaggacaac
     1801 tttgggtaaa atacctaggt tgtaatacgg tcattgttgc caatgacgaa gtaagcacgg
     1861 acaagatgca acaaactctg atgaaaacag ttgtgccaga ctcagttgcc atgcgtttct
     1921 tccctttgca aaaggtgatt gatatcattc acaaggctaa tcctgctcaa acgatcttta
     1981 tcgttgtaaa ggatgtgaag gacgctttaa ccttggtaga aggtggtgtc cctatcaaag
     2041 aaatcaatat tgggaacatt cacaatgcta ctggtaaaga gcaagtgaca cgctccatct
     2101 tcctgggtga agaggacaag gcagccctca aggaattgag ccaaactcat caagtaacat
     2161 ttaatacgaa aacaactcca acaggaaatg atggagctgt tcaagtcaac attatggact
     2221 atatttaaaa gaggagatcg ttatgtcgat taatgtattt caagcgattt taattggatt
     2281 atggacagct ttctgtttta gtggaatgct gttagggatt tacaccaata gatgtattgt
     2341 tttgtcattt ggtgtcggaa ttattctagg tgatttgcct actgctcttg caatgggagc
     2401 tattggtgaa ttggcttata tgggattcgg tgttggtgct ggaggtactg ttccaccaaa
     2461 cccaatcgga cctggtatct ttggtacctt gatggctatc actagtgctg gtaaagtcag
     2521 tccagaagcg gctcttgccc tctctactcc gattgctgtg gcgattcaat tcttacaaac
     2581 tttcgcctac actgtacgtg ctggtgcgcc tgaaacggcg atgaagcact tgaaaaacca
     2641 taatttgaag aaattcaagt tcactctaaa tgcaacaatt tggttgtttg cctttattgg
     2701 atttaccttg ggttgcttgg gtgccctttc aatggatacc ttgttgaaac tcgtagacta
     2761 cattccaccg gtattgctta caggtttgac agttgctggt aaaatgctcc cagctatcgg
     2821 ttttgcgatg atcttgtcag tgatggctaa gaaagagttg attccctttg tcttgttggg
     2881 atatgtttgt gcagcttatc taaacatccc aacaattggt attgcaattg taggtactat
     2941 ctttgctttg attgaatttt ataacaagcc aaaaacagcg gatcatgtgg tagaggagga
     3001 agcacacgat gactggatct aacaaattaa caaaacgtga ttatcttaaa acgtctttgc
     3061 gggcattctt ttgtcaaaat ggatttaact atagtaacta tcaagggttg ggatatgcca
     3121 atgtgatgta tcctgctttg aaaaaacact atggagagga tcaggaaggt ttctaccaag
     3181 ccttggaaga aaactgtgaa ttctataata ccaacccaca cttcctgcct tttattacca
     3241 gcttgcatct tataatgttg gaaaatggcc gtccggcaaa agaaacacgt agcatcaaga
     3301 tggccttgat gggaccattg gcaggtattg gggattctct ttctcaattc tgtttagctc
     3361 cattgttctc aaccatcgca gcttcgtttg ctcaagaagg cttggttgtc ggtccaatct
     3421 tgttcttcct tgcgatgaat acgattttaa cagcgattaa attgtcaact ggtctgtatg
     3481 gatacaaact aggaacaact gtgattgata aactaagcga gcagatggca acgatttctc
     3541 gtattgccaa tattatcggt gtaaccgtaa ttgctggttt ggcagcgaca tctgttaaga
     3601 ttatggtgcc gattaccttt gctgcagggg aagttaaagc agacgctaaa caaagtatcg
     3661 taagtattca gggaatgctt gataaggttg ctccagctct tctaccagcc ctatttacac
     3721 ttttagttta ctacttgatc aaagaaaaga aatggacaac atataaactc gttattttaa
     3781 cagttatcat cggaattatc ggaagctggc ttaagattat agcttaatgg atacaacatt
     3841 gttttatgga atagtgattg tcttggcagt gagtcccctt ttactgtcaa gctttcattc
     3901 tattcgtcag caaaagttgc ttcgcaaaca gatggagcaa cgacaagagt atttagcttc
     3961 tttaacatct ggtgatgaag tgttgttatt gtctggaatt catggaaaaa tcatttctat
     4021 caaagatgac ttggtctcct tgcagattgc aaaaggtgtg gtcatctatg tagaaaagga
     4081 aagtgtaatg ggaaagacaa aagaactgct ttttaagtag ttctttttct ggtaatagat
     4141 aatgaaagaa gaaagaagac aattttttga aagagtcgat ggaaaccaat gtcgtgatta
     4201 tatcttgtcc cactgttcaa aagactatga gaaagtcaag agttcccttg aacgcttgat
     4261 ggacaatcgt tttatgtttg atagtccttg ggatatggag ccttgttcaa aaatccatca
     4321 aatccagccg atggtatggg atcaagtatt tgaagatgat ccagaatggt cttatatgct
     4381 caatcgacaa gaatatctct tgcagtttat gatagggtat ctggtagaag gagataagga
     4441 ctatattcaa aagtgcaagt tctttctatt tgattggatt gagcaggtga gagaattttc
     4501 tcctcaatcc ttgatgacta gaaccttgga tacgggtatt cgttccttta cttggttgaa
     4561 actactcttg ctcctcttga aatttgactt gctagaggag aaagaactag agaaaatttt
     4621 ggtcagtcta gaaaagcaga ttgactttat gaaaagccac tatcgcgcca agtacaccct
     4681 tagtaactgg gggattttac aaacaattcc gatgcttgct atctatcatt tcttttcaga
     4741 taagatggac ctagaagaag cttaccattt tgcttcagag gagttgaaac agcaaattga
     4801 gacacagatt ttaggagatg gaagccagtt tgaacagtcg attctctatc atgtagaggt
     4861 ttataaagcc ttgctggatt tgtgtctctt gcttccagat ttgcaagata gttaccaaga
     4921 gttgctggaa aagatggcga cctatattca aatgatgaca ggcttagatg gacggacttt
     4981 ggcttttggt gatagcgatt ctacagaaac gacagaaatt ttgagcctgt ctgctgtggt
     5041 tttgaacaag gaagaccttc ttaacggtct ggatgttaaa gtcgatttgc tcagcctctt
     5101 gttcctgggg cgagaaaagg tcaagcgact gcaggaattt gaaaagagag cttggcagcc
     5161 taagtccatg atctttgaag actctggaca tgtctgcatt aaggatgaac atcgttatct
     5221 atttttcaaa aatggtccgc taggaagtgc ccatagccat agtgacgaga atagtttttg
     5281 cttacagtat caaggccaac ctattttcat agatgctggg cgttattctt atcgggagat
     5341 atatgaacgt tatctcttaa agagtgcttg gagtcattcg acctgcattg tagatgggaa
     5401 agctccggaa agaatcacgg gatcctggga atatgaatac tatcctcact ccctgttttg
     5461 tcaccataaa gaaagggagg gaatgcatta tattgaaggg gcttattggt cagcagaacc
     5521 tgatttgcct tatcttcaca ggagaaaaat cctcatgttg gtagaggatg tctggctctt
     5581 ggtagatgac atcaggtgtc aaggtcagca tgaggcgttg actcagttta tccttgacaa
     5641 ggatgtgacc tatcaagatg ggaaaatcaa tcagttgaga ctatggagtg aagttgattt
     5701 tgatttggaa gataccatca tttctcctaa atacaatgag cttgaaagaa gtagcaaact
     5761 caccaagcgc caattctttg agaatcagat gctggattat accatcattg cgcatgagag
     5821 ttttgaaatc atccgtcatt ctgtctacca gacagatgat cgtgaagtgg aaaatgctct
     5881 ggcttttgaa gtgaaaaatg acgaaacaga caagctgatt ctgttattaa gcgaggatat
     5941 ttgtgtaggt gaaaaattgt gcctcgttga cggaacaaaa atgcgtggaa aatgtctagt
     6001 atatgataaa ataaatgaga gaatgattcg cttgcagtgc tagaaatagg cattttgaat
     6061 agtgaatatg ttataataag tattagtagg aggtgtttta gattggagaa gaaactgacc
     6121 ataaaagaca ttgcggaaat ggctcagacc tcgaaaacaa ccgtgtcatt ttacctaaac
     6181 gggaaatatg aaaaaatgtc ccaagagaca cgtgaaaaga ttgaaaaagt tattcatgaa
     6241 acaaattaca aaccgagcat tgttgcgcgt agcttaaact ccaaacgaac aaaattaatc
     6301 ggtgttttga tcggtgatat taccaacagt ttctcaaacc aaattgttaa gggaattgag
     6361 gatatcgcca gccagaatgg ctaccaggta atgataggaa atagtaatta cagccaagag
     6421 agtgaggacc ggtatattga aagcatgctt ctcttgggag tagacggctt tattattcag
     6481 ccgacctcta atttccgaaa atattctcgt atcatcgatg agaaaaagaa gaaaatggtc
     6541 ttttttgata gtcagctcta tgaacaccgg actagctggg ttaaaaccaa taactatgat
     6601 gccgtttatg acatgaccca gtcctgtatc gaaaaaggtt atgagcattt tctcttgatt
     6661 acagcggata cgagtcgttt gagtactcgg attgagcggg caagtggttt tgtggatgct
     6721 ttaacagatg ctaatatgcg tcacgccagt ctaaccattg aagataagca tacgaatttg
     6781 gaacaaatta aagaattttt acaaaaagaa atcgatcccg atgaaaaaac tctggtattt
     6841 atccctaact gttgggccct acctctagtc tttaccgtta tcaaagagtt gaattataac
     6901 ttgccgcaag ttgggttgat tggttttgac aatacggagt ggacttgctt ttcttctcca
     6961 agtgtttcga cgctggttca gcccgccttt gaagaaggac aacaggctac aaagattttg
     7021 attgaccaga ttgaaggcct caatcaagaa gaaaggcaac aagtcttgga ttgtagtgtg
     7081 agttggaaag agtcgacttt ctaagatgaa agggaaataa ctggcaacct ctgttaagaa
     7141 ataaaataat cccatctagc atgagctgga tgggattatt tgtctataaa atgagaaatt
     7201 acgggagtaa tctcctaaat caactgtttt tgatttactt ctttaactac ttgataaaag
     7261 ttatagaagt agaccaaact tgagatgatg gttacgacta ggaatattgg aaatttccat
     7321 tggatggagt ttcttgattg tcttgattca agactgaaaa atcgtatagt gaagtcattg
     7381 cttgcttttc tccttctgtt tggtattttt aggagttttc tcctcctgta gtgatagaaa
     7441 tccgtaggtc agggttccga aaatactacc gataatcatg ccatacatca ggaaaggaac
     7501 acttttatca aattgcatga gcaacttccc aaaatctgaa agggacatct gctgaacgaa
     7561 gactttatga aagatgagta gggtaaagag gccaatctga aggccgataa acacaaccca
     7621 gcttcgaaat ttgatttggg ctttgctgta ggttttgagg atgatttctg acttgtcatc
     7681 tttttccaga tggagttctt tgacgactct ttgagttttt aaagtaatga aaaaaatgag
     7741 tccagagtaa acgtagggca tggcatagcg aacggattct atgaagcgtc caaataacag
     7801 ataaaacaag aagaggaaga aggaagaata aacgatttgg accatggaac gggcacaagc
     7861 cttgtagata atctcttgtc ggcattcatc aaaagggcct tggatatgaa agagatttcg
     7921 aagtaggcga gtggtgaagt cttctttttt catactagta acctcctaaa tgagcggttt
     7981 tactgatttt cttttacgaa ttgatagaga taataaagat aagtgataga agaaaggcta
     8041 gcaatagtca gcagtaaata atatttccaa ccgcttgaga tgagcatcag ttgtggaaaa
     8101 gatagaatca taaagcacag tgctaagttg agtatgcgtg ttttttggga gtcaatcttt
     8161 agataagtta gtcctttgtt gagggctgga ataaagacta gaaggaggaa tatctcgctg
     8221 aatggcaagt tccctgaaac gatgatgaag atgagaagag aactgaacaa aagatgatag
     8281 gtaagtaaag ttagtaatga tttcataggt acctcctaat cctggtcttt tttagctctt
     8341 gcaatacgaa gtgagtcgac aatatgcatc atcactccga aaaagaaagc tcccagtata
     8401 gttttaaaaa tatgttttgt atttagaaga gaagtgataa aatttggatt ttcacttgtt
     8461 agggtatcaa tgagtggaat tataaaaaat atcactattc cataaatcga acctgctttc
     8521 agaccaggat gacgtaactg tttcttttct tttttcatga gtttcctcct aatcattatc
     8581 ttgatttttc ttggcttttg caatgcgacg ggagatgaga atctgtataa ccactccgaa
     8641 aaagacagac tggaggatgc ttgaaaaaat atttttaaaa gcgagaagtg actgaagata
     8701 gtcctgactc tcacctagca gtataaggag aagaggggtt gaaaaaaaac tcattaatcc
     8761 atagactatt ccagctttca gaccagggta atgtagttgc ttactttctt tctcactcag
     8821 catatctggt tcaatagttg taatccctgt tttttgcatt tggtaggtga gatatccagc
     8881 aacgatgagg gcaatcacta aaatcagagg aggatagact agagccacta gttgaggata
     8941 tttataggcc agaacgagcg gaataagatt gccgaaaatc atcagataaa ataggaagat
     9001 aaagacttga ttgccgatac gatcagcctc gcgtcgtttg tattcgtcaa gtggatcaga
     9061 aataccgtat gtgcgcttga tcagtttttc cgtgaaggtt tcttttttca tgagttggct
     9121 ccttttttaa aaattatcct cccaaaagag actgttgagg tcagtttgga ggctgcgggc
     9181 gagattgaga cagagttcca gagtcggatt gtacttgtcg ttttcaatca tgttgatggt
     9241 ttgtctcgag acaccgatat ccttggcgag ctcgagctgg gaaatgccca gttccttgcg
     9301 aaattctttc acacgattca tttgttctcc tttctgattt atgtcgcata tatttgacta
     9361 tattatattc ttctatagag agaatgtcaa gcatatttga catatttctt aaagaaatct
     9421 tacttgtttt tggctgattt gtctgactag tgcaagctag tcagatttgt ggtaaaatag
     9481 ataagatatg acaaaagaat ttcatcatgt aacggtctta ctccacgaaa cgattgatat
     9541 gcttgacgta aagcctgacg gtatctacgt tgatgcgact ttgggtggag caggccatag
     9601 cgaatattta ttaagtaaat taagtgaaaa aggccatctc tatgcctttg atcaggacca
     9661 gaacgccatt gacaatgcgc aaaaacgctt ggctccttac attgaaaagg gaatggtgac
     9721 ttttatcaag gataacttcc gtcatctaca ggcacgtttg cgcgaagctg gtgttcagga
     9781 aattgatgga atttgttatg acttgggagt gtctagtcct caattggatc agcgtgagcg
     9841 tggtttttct tataaaaagg atgcgccact ggacatgcgc atgaatcagg atgctagtct
     9901 gacagcctat gaagtggtga atcactatga ctatcatgac ttggttcgta ttttctttaa
     9961 gtatggcgag gacaaattct ctaaacagat tgcgcgtaag attgaacagg cacgtgaagt
    10021 caagccaatt gagacaacga ctgagttggc agagattatc aagtcggcca aacctgccaa
    10081 ggagctcaag aagaagggcc atcctgccaa gcagattttt caggctattc gaattgaagt
    10141 taacgatgag ctgggggcgg cagatgaatc tatccagcag gctatggaga tgctggctct
    10201 agatggtaga atttcagtga ttacctttca ttccttagaa gaccgcttga ccaagcaatt
    10261 gtttaaggaa gcttcaacag tggaagttcc aaaaggtttg cccttcatcc cagatgacct
    10321 caagcccaag atggaattgg tatcccgtaa gccaatcttg ccaagtgcag aagaattaga
    10381 agccaataac cgctcgcact cagccaagtt gcgcgtggcc agaaaaattc acaagtaaga
    10441 ggaaaaaatg gtagataaga aagaaacaac cagtcaattc ttgcagagtc gtataaaaaa
    10501 attctcccgt gtggagaagg ctttttatct ttccattgcg tttacggctc tcgtcttagc
    10561 agtgagtatt atctttatgc agacacgact cttgcaagtg caaagtgatt tgacaaaaat
    10621 caatgcacag atagaagaga agaagacaga gctcgatgat gctaagcagg aagtgaatga
    10681 attgattcgt tcagaacgct tgaaagaaat cgcaaattct aaggacttaa aattgaataa
    10741 cgagaatatt cgatcagcgg agtaagatat gaagtggaca aaaagaataa cccgatttgc
    10801 gattagaaac cgtaaatctc cagcagaaaa ccgtaaaata gtagggaagt acatcagctt
    10861 gttagctgtc gtacttttcg ctgtcttttt ggtcaatttt gctgttatta tcgggagtgg
    10921 tagtaaattt ggaacggatc tagtaaaaga ggctaagaaa gttcaccaaa taactcgtac
    10981 agtccctgcc aaacgtggga ctatttatga ccgcaatgga gtcccgattg ctgaggacgc
    11041 aacctcctat aatgtctatg ctgttattga taaaaaatac aaatcagcaa cgggtaaaat
    11101 tctttatgta gaagatgccc agtttaataa ggtagcagag gttttccata agtatctgga
    11161 tatggacgag gcttatgtta aagaacaatt gtctcaaccg aatctgactc aagtttcttt
    11221 tggtgccaaa ggaaatggaa ttacctatgc caatatgatg gctattaaaa aggatttgaa
    11281 agacgctagt gttgaaggaa ttgacttcac aactagccct aatagaagct atccaaatgg
    11341 acaattcgct tctagtttta ttggtttggc ccaactccat gaaaatgagg atggtagcaa
    11401 gagtttgctg ggaacttctg ggatggagag ttccttgaac agtattcttg cagggaaaga
    11461 cggtattatt acctatgaaa aagatcgtct gggtaatatt gtccctggaa cagaacaagt
    11521 ttcccaacaa acggtagatg gcaaggatgt ttatacaaca ttgtctagtc cgctacaatc
    11581 tttcatggaa actcagatgg atgcctttct agaaaaagta aaaggtaagt atatgaccgc
    11641 gaccttggtc agtgcaaaga ccggtgaaat cctcgctacc acccaacgac ctacctttaa
    11701 tgcagatact aaagaaggaa tcactgagga ctttgtttgg cgtgatattc tttatcaaag
    11761 taactatgaa ccaggatcag ccatgaaggt tatgacgtta gcttcttcta ttgataataa
    11821 taccttccca agtggagaat acttcaatag cagtgaattc aaaatagcgg atgcgacgat
    11881 tcgagattgg gatgttaatg aaggtttgac tactggtggg atgatgactt tcttacaagg
    11941 tttcgctcac tccagtaatg ttggaatgag tctacttgaa caaaaaatgg gagatgctac
    12001 ttggttggat tatctaaaac gctttaaatt tggagttcca actcgctttg gcttgacaga
    12061 tgaatacgct ggtcaacttc cagctgataa tattgttagt attgctcaaa gctcatttgg
    12121 gcaaggaatt tcagtgacac aaacacaaat gcttcgtgcc tttacagcta ttgctaatga
    12181 tggagttatg ctggagccaa aatttataag tgctatttat gatactaaca atcagtctgt
    12241 acgtaagtca caaaaagaaa tagtaggaaa tcctgtttcc aaagaggcag caagcacaac
    12301 tcgaaatcac atgatcttag ttgggacgga ccctctatat ggaactatgt ataatcacta
    12361 cacaggaaag ccaattataa cagttcctgg acaaaatgta gcagttaaat ccggtacggc
    12421 tcaaatcgct gatgagaaaa atggaggata cttggttggt tctaccaatt atattttctc
    12481 agttgtgact atgaatcctg ctgaaaatcc tgattttatc ttgtatgtaa cggttcaaca
    12541 gcctgagcat tattcaggta tccagttggg agaatttgcc accccaatct tggagcgggc
    12601 ttcagctatg aaagaatctc tcaatcttca atctccagcc aaaaatttag ataaagttac
    12661 gacagaatct tcttatgcaa tgcctagcat caaggatatt tcacctggtg agttggcgga
    12721 agccttacgc cgaaatattg tgcaaccaat cgttgtaggt actggaacaa agattaaaga
    12781 gacttctgta gaagaaggga ccaatcttgc accaaaccaa caagttctcc ttttatcgga
    12841 taaggtagaa gaaattccag acatgtatgg ctggaaaaaa gagactgccg agacctttgc
    12901 taaatggttg gatattgaac tggaatttga aggttcaggt tctgttgttc agaagcaaga
    12961 cgttcggact aatacagcta ttaaa
//
LOCUS       DAVGJL010000051        12902 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.051, whole genome shotgun sequence.
ACCESSION   DAVGJL010000051 DAVGJL010000000
VERSION     DAVGJL010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12902
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.051"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(383..1609)
                     /locus_tag="VVE42_001174"
     CDS             complement(383..1609)
                     /locus_tag="VVE42_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEU3073374.1"
                     /translation="MPNYNIPFSPPDITEAEIAEVADTLRSGWITTGPKTKELERRLS
                     LYTQTPKTVCLNSATAALELILRVLEVGPGDEVIVPAMTYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVELAGIVCDYDRLFQVVEKKRDFFTASSKWQK
                     AFNRIVIVSDSAHALGSTYKGQPSGSIADFTSFSFHAVKNFTTAEGGSATWKANPVID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYP
                     SLLQRRKDIVDRYDSGFAGSRIHPLAHKTETVESSRHLYITRVEGASLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMTNYPKAYAFFENEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTLSKK"
     gene            complement(1652..2344)
                     /locus_tag="VVE42_001175"
     CDS             complement(1652..2344)
                     /locus_tag="VVE42_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEU3073375.1"
                     /translation="MLKWEDLPVEMKSSEVESYYQLVSKRKGSLIFKRCLDWVLALVL
                     LVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGSLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMMATLLL
                     QAGITSPASINYKDEDTIISQMTEKGLSVDQAYVEHVLPEKMRYNLAYLREFSFFGDI
                     KIMFQTVFEVLK"
     gene            complement(2517..4268)
                     /locus_tag="VVE42_001176"
     CDS             complement(2517..4268)
                     /locus_tag="VVE42_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3073376.1"
                     /translation="MQNKQEQWAVLKRLMSYLKPYGLLTFLALSFLLATTVIKSVIPL
                     VASHFIDQYLSNLNQLAVTVLLVYYGLYILQTVVQYVGNLLFARVSYSIVRDIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTALVLLFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQAEFDEINQEHLVYANRSVALDALFLRPAMSLLKLLGYAVLMAYFGYRGFSIG
                     ITVGTMYAFIQYINRLFDPLIEVTQNFSTLQTAMVSAGRVFALIDERTYEPLQENGQA
                     KVQEGNIRFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGRVLLDDVDIRDFSQEELRKNIGLVLQEPFLYHGTIKSNIAMYQETSDEQVQAAA
                     AFVDADSFIQELPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKILILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELLALGG
                     TYHKMYSLQAGAMADTL"
     gene            complement(4258..6003)
                     /locus_tag="VVE42_001177"
     CDS             complement(4258..6003)
                     /locus_tag="VVE42_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3073377.1"
                     /translation="MSIIQKLWWFFKLEKRRYLVGIVALVLVSVLNLIPPMVMGRVID
                     AITSGQLTQQDLLLSLFYLLLAAFGMYYLRYVWRMYILGTSYCLGQIMRSRLFKHFTK
                     MSSAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYATSRLGRKTHKAFGESQAAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLVFVGSSYVLTLLVGSLMVQEGQITVGN
                     LVTFISYLDMLVWPLMAIGFLFNTTQRGKVSYQRIENLLSQESPVQDPEFPLDGIENG
                     RLEYAIDSFAFENEETLTDIHFSLAKGQTLGLVGQTGSGKTSLIKLLLREYDVDKGAI
                     YLNGHDIRDYRLTDLRSLMGYVPQDQFLFATSILDNIRFGNPNLPLSAVEEATKLARV
                     YQDIVDMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILDPDILILDDSLSAVDAKTEY
                     AIIDNLKETRKDKTTIITAHRLSAVVHADFILVLQNGQIIERGTHEDLLAMDGWYAQT
                     YQSQQLEMKGEEDAE"
     gene            complement(6209..6505)
                     /locus_tag="VVE42_001178"
     CDS             complement(6209..6505)
                     /locus_tag="VVE42_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEU3073378.1"
                     /translation="MSASDVALKLRSQGIFQMKQVKRAVQEQNGQLIVVQMGDENPKY
                     PVVTDGVIQVDVLESIGRSEEWLLDNLSKQGHDNVANIFIAEYDKGAVTVVTYK"
     gene            complement(6658..7113)
                     /locus_tag="VVE42_001179"
                     /pseudo
     CDS             complement(6658..7113)
                     /locus_tag="VVE42_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 family protein"
     gene            7247..8074
                     /gene="xth"
                     /locus_tag="VVE42_001180"
     CDS             7247..8074
                     /gene="xth"
                     /locus_tag="VVE42_001180"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEU3073379.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQE
                     TKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTISFPEI
                     GAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDVKYAEYLAELDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLTTGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL"
     gene            8086..8229
                     /locus_tag="VVE42_001181"
     CDS             8086..8229
                     /locus_tag="VVE42_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEU3073380.1"
                     /translation="MDYNAVIPELLVSNIEQSRSFYCGLLGFRIEYQRPEENFLFLLK
                     SVN"
     gene            8229..8468
                     /locus_tag="VVE42_001182"
     CDS             8229..8468
                     /locus_tag="VVE42_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEU3073381.1"
                     /translation="MLEEGTKDQLAELTYPFGRGVNLSFGIKDVPKLYQKVMEANYPI
                     YRPLTKRKFRVSDPYIYPHKFAVLDPDGYFLRFSE"
     regulatory      8851..8946
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            9346..9927
                     /locus_tag="VVE42_001183"
     CDS             9346..9927
                     /locus_tag="VVE42_001183"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEU3073382.1"
                     /translation="MKLLEERILKDGHILGDNILKVDSFLTHQVDFSLMREIGKVFAE
                     KFAATGITKVVTIEASGIAPAVFTAEALNVPMIFAKKAKNITMNEGILTAQVYSFTKQ
                     VTSTVSIAGKFLSPEDKVLIIDDFLANGQAAKGLIQIIEQAGATVQAIGIVIEKSFQD
                     GRDLLEKAGYPVLSLARLDRFENGQVVFKEADL"
     gene            9927..11189
                     /locus_tag="VVE42_001184"
     CDS             9927..11189
                     /locus_tag="VVE42_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEU3073383.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSAEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGSGAMFGAL
                     IASGIYVVLVSGIFSKVANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LAAITVLIILLINIFTKGFIKSISILIGLVVGTAIAATMGLVDFSPVAVAPLVHVPTP
                     LYFGMPTFEISSIVMMCIIATVSMVESTGVYLALSDITKDPIDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTGFSQNVGLVKLSGIKKRLPIYYAAGFLVLLGLLPKFGALAQIIPSS
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIVVASLLAIVLNAVLNHKKK"
     gene            complement(11276..12142)
                     /locus_tag="VVE42_001185"
     CDS             complement(11276..12142)
                     /locus_tag="VVE42_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-2 restriction enzyme DpnII"
                     /protein_id="HEU3073384.1"
                     /translation="MKQTRNFDEWLSTMTDTVADWTYYTDFPKVYKNVSSIKVALNIM
                     NSLIGSKNIQEDFLDLYQNYPEILKVVPLLIAKRLRDTIIVKDPIKDFYFDFSKRNYS
                     IEEYTMFLEKSGIFDLLQNHLVSNLVDYVTGVEVGMDTNGRKNRTGDAMENIVQSYLE
                     AEGYILGENLFKEIEQNEIEEIFSVDLSAITNDGNTVKRFDFVIKNEQVLYLIEVNFY
                     SGSGSKLNETARSYKMIAEETKAIPNVEFMWITDGQGWYKAKNNLRETFDILPFLYNI
                     NDLEHNILKNLK"
     gene            complement(12129..12899)
                     /locus_tag="VVE42_001186"
     CDS             complement(12129..12899)
                     /locus_tag="VVE42_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEU3073385.1"
                     /translation="MTKPYYNKNKMILVHSDTFKFLSKMKPESMDMIFADPPYFLSNG
                     GISNSGGQVVSVDKGDWDKISSFEEKHEFNRKWIRLAKEVLKPNGTVWISGSLHNIYS
                     VGMALEQEGFKILNNITWQKTNPAPNLSCRYFTHSTETILWARKNDKKARHYYNYDLM
                     KELNDGKQMKDVWTGSLTKKVEKWAGKHPTQKPEYLLERIILASTKEGDYILDPFVGS
                     GTTGVVAKRLGRRFIGIDAEKEYLKIARKRLEAENETN"
BASE COUNT         3822 a         2817 c         2509 g         3754 t
ORIGIN      
        1 tcatgcaaaa gaatgactag aacatgaaag acaggcacaa aactttgaac aatattctga
       61 aaacaaaaaa aagacgcgag tttccacaat ctcactcatg gaaactcacg tctttcaaaa
      121 aatagggtga attgaaaact agaacaagac actagatttt ctaaatgatg tctagtaagg
      181 attctaaatg ctacaccaaa ttgctcacat cttatttcaa accacgggac tcccacaaga
      241 aaggaaaatc ctataaaaat tctctctctg aggagaaagg agcctcaact acaggtgtaa
      301 actattatca attgaaaaca acagttttta cactcaatta gactttttag ggacaggatc
      361 actttcgctt gactgtagtt tgtcattttt ttgataaagt tagcactttt tcagaaactg
      421 ttttgaaagt ctcaatgata tagtctactt cttcatcgct taatttagta tgaagaggga
      481 gggtaatttc attctcaaag aaggcatagg ccttaggata gttcgtcata tcaaatccaa
      541 gattcttata ggctgtcaag agaggaagcg gtttgtagtg aacattactt gcaattcctg
      601 ctttagccaa ttcttggatg atgaggttgc gttcttctag gcttgctcct tctacacggg
      661 tgatgtagag gtggcgtgaa gattcgacag tttcagtctt gtgtgccaaa ggatggatgc
      721 gagaacctgc aaaaccacta tcatagcggt ccacaatgtc cttacggcgt tgtaacaaac
      781 ttggatagcg gtccaattgt accaaaccaa gtgaagccat gatatcggtc atgttgcact
      841 tataggctgg tgtaacgata tcgtattccc atgaccccaa ttgcatcttg gcaagagcat
      901 ccttagtttg cccgtgaagg gaaaggattt ggaattcctt gtacatctct tcgtcatcaa
      961 tcactggatt ggctttccaa gtcgcacttc caccttctgc cgttgtaaag ttcttaacgg
     1021 catggaatga gaaggaagta aaatcagcga tagaaccaga aggttgtcct ttataagtag
     1081 atcccaaagc gtgggcacta tcagagacaa tgacaatacg gttaaaggcc ttttgccact
     1141 tgcttgaagc ggtaaagaag tcacgttttt tctccacgac ttggaacaaa cggtcataat
     1201 cgcaaacaat ccctgcgagc tctactggga taatcacctt agttttctca gtgatagctt
     1261 gctcaagcag gtcatagtcc atctcaaacg tatctgcttg gatatccacc atgacagggg
     1321 ttgctcccac gtgcgtaatg acactacatg aagccgtata ggtcatggct ggaacgatga
     1381 cttcatcacc aggtcccact tccaaaacgc gtaaaatcaa ctccagagcg gctgtcgcag
     1441 agttgagaca aacagtctta ggtgtctgtg tgtaaagaga caagcggcgc tccagttctt
     1501 ttgttttagg acctgttgtg atccaaccag aacgcagggt atccgctact tcagcaattt
     1561 ctgcttctgt gatatcaggc ggtgaaaatg gaatattgta atttggcatt gatttgctcc
     1621 ttttatctgt actcattttc ttatgactac tttattttag tacctcaaac acggtttgaa
     1681 acatgatttt gatgtcccca aagaaactaa actctcggag ataggcgagg ttatagcgca
     1741 tcttttcagg aagaacatgc tccacatagg cctgatcaac tgacagacct ttctccgtca
     1801 tttgactgat gatggtgtcc tcatccttgt agttgatgct ggctggagag gtaattcctg
     1861 cttgcaagag caaggttgcc atcatttcag ggctatactg ctctgtataa cgtggcactt
     1921 caggtcgtgt accgacaaag gacatctcac ctttaaggac attgaccaac tgaggcagtt
     1981 cgtccaaacg gacacgtcgg atgaaatttc caaccttggt aatgcggcta tcgttagcag
     2041 aagtcaccag acttcctttt ttatccgcat ccgtcaccat ggtacgaaac ttccaaatct
     2101 tgaaccgacg gttgtactgg gtcacacgct cttgcttgta aatcactggc cctttgctat
     2161 ccaacttgat ccaaatgctc aagatgagaa agatgggaga ggtcagaacc agtaagacca
     2221 aggccaaaac ccagtccaag caacgcttga aaatcagcga acccttcctt ttagagacaa
     2281 gctggtagta agactcaacc tcgcttgatt tcatttccac aggcaagtct tcccatttca
     2341 gcatgctgtt ctcctttgtt tttattatac tatttgtcaa catttttcat tataccacaa
     2401 aatggaaaag gcggtgaaag aaatctgtaa tttgaaggat ttccttctag tactcaatga
     2461 aaatcaaagt acagctttga gattgcagat aaagctgaca tggtttaaag agattttcaa
     2521 agagtatcgg ccatggcccc tgcctgcaaa ctatacatct tgtgataggt tcctcccaga
     2581 gccaagagtt cctcatgggt tccactctcg ataatgcgtc ccttatccaa gacatagatg
     2641 cagttggcat cttgaatagt agaaaggcgg tgagcgatag caatagttgt tcggccctgt
     2701 ctcatcttcg ccagagaagc ttgaaccaag ctttctgttt cagagtcaat attggctgtc
     2761 gcttcatcca aaatcaggat tttaggctgg ctggcgactg ttctagcaaa ggcaagaagc
     2821 tggcgttgcc cagtagagaa gctcgaacca cgctcggaaa caggggagtc gtacccctga
     2881 ggaagttctt gaataaagga atctgcatcc acaaaggctg ccgcagcctg aacctgctca
     2941 tcactggttt cttggtacat ggcgatattg gacttaatag ttccatgata gaggaagggt
     3001 tcctgcaaga ccaaaccgat gttttttctc agctcttctt gactgaaatc cctgatatcc
     3061 acatcatcca agagaactct ccctgactgg aattcataaa agcgcatgag gacattgata
     3121 atagacgatt tccctgaacc tgtatgacct acaaaggcaa tggtttcacc cttattaaca
     3181 gagaaagaaa tgtcatccag aatcggatgt ttaccgtcat atgagaaaca cacatgttca
     3241 aaacggatat tgccttcttg gactttggct tgcccatttt cttgaagagg ttcataggtc
     3301 ctctcgtcta tcagggcaaa gacacgacct gcagaaacca tagccgtttg cagagttgaa
     3361 aagttttgcg tcacctcaat caaggggtca aaaaggcggt tgatgtactg gataaaggca
     3421 tacatggtcc cgaccgttat cccgatagaa aaaccacggt agccaaagta ggccatcaag
     3481 actgcatagc ctagaagttt cagcaaactc atggcaggtc tcaaaaagag ggcatccaag
     3541 gctacagaac ggttggcgta gaccaagtgt tcttggttga tttcatcaaa ttctgcctgc
     3601 aggcgcttct cttgattaaa ggcctgaata atcctgattc cctcgatatt ctctgccagc
     3661 ttactattga tatctgacaa gagacttctg gttttctcga tgattttcac tgactttttt
     3721 cgatagagat tgaccaaaag gaaaatcaaa ggaagaaaga gcaagactaa agccgtcaaa
     3781 cgaaaatcca gcaccaacat ggtataaagg gttgtcagaa agataaaaac tgctgagata
     3841 aagctggata aaatcccaga aaacatatca ctaatcgtct cggtatcgtt ggtcaaacga
     3901 gaaacgatag aacctgctgg cgtcttgtca aagtaagaca tgcccagttt ctccatatta
     3961 gcaaaggcat ctcgacgaat atccctaaca atactataag acacgcgcgc aaagagaaga
     4021 ttgcccacat actgaactac agtttgtagg atgtagagac catagtagac cagcaaaacg
     4081 gtaacggcta gttggttaag attgctgaga tactggtcga taaagtggga agccacgagg
     4141 ggtatgacac ttttaatgac cgtcgtcgct aggagaaaac tgagtgccaa aaaggtcagg
     4201 agtccataag gcttgagata agacatcaag cgcttcaata cagcccattg ttcttgttta
     4261 ttctgcatct tcttctcctt tcatttccaa ctgctgagac tggtaggttt gggcatacca
     4321 gccatccatc gctagcaagt cttcgtgcgt gcctcgttcg ataatttgac cattttgtag
     4381 aactaaaata aaatctgcat ggacaacagc actgaggcga tgggcagtga taatggttgt
     4441 cttgtccttt cgcgtctcct tgaggttgtc gataatcgca tactctgtct tggcatctac
     4501 ggcggataag gaatcatcca aaatcaagat atcagggtct aaaatcatag cccgactcat
     4561 agccaaccgt tgcttttgac caccagaaag actgactcct ttttcaccaa tcagcgtatc
     4621 aaatccttga ggcatgtcta caatatcttg gtaaacccgg gctagcttag tagcttcctc
     4681 gaccgctgaa aggggcaagt taggattgcc aaagcggata ttgtctagga ttgaagtcgc
     4741 aaaaagaaac tggtcctgag gaacatagcc catgagactg cgaaggtctg tcagacgata
     4801 gtcccgaata tcgtgaccgt ttagataaat ggcaccctta tccacatcgt attcacgcaa
     4861 gaggagcttg attaaggacg ttttcccaga gcctgtctgc ccaaccaagc ccagtgtttg
     4921 cccttttgcc aaactaaagt gaatatccgt cagtgtttcc tcattttcaa aagcaaagct
     4981 gtcaatggca tactccaaac gcccattttc aataccatcc agaggaaact cagggtcttg
     5041 tacaggagat tcctgagaca aaagattttc aatccgctgg taagaaacct tccctcgctg
     5101 agtagtatta aagaggaagc cgatggccat aagaggccag accagcatat ccaaatagct
     5161 gataaaggtg actagattcc caactgtaat ctgcccttcc tgaaccatca aggagccaac
     5221 caaaagcgtt aaaacatagg acgaaccaac aaacacgaga accatagggt caaagagact
     5281 atcatatttc atggtttgca ggttcttttg gaaggttaat tcattgactg cctgaaaaga
     5341 cttcaactcg tctgcctgat aaccgaaaga cttggtcact ttgatacctg atacggactc
     5401 ctgtaccttg ttattgagtt cagaaaaagc agcttgggat tcgccaaagg ccttatgagt
     5461 ctttctccct aggcgactag tcgcataggc catgaaaggt agggggagaa tggcaacaag
     5521 agtcatctgc catgagatgc taaagagcat ggtcaacaaa gtcaccagag ccgtgataga
     5581 ggcatccacc gcagacatga caccgccacc tgctaaacga gtcaaggcat tgatatcatt
     5641 ggttgcgtgt gccatcagat cacccgtccg ataggtttga taaaaggctg atgacatttt
     5701 tgtgaaatgc ttaaacaagc gagaccgcat gatctgtccc aagcaataag aggtcccaag
     5761 gatatacata cgccacacat agcgcaaata gtacatacca aaggctgcaa gtagcaagta
     5821 aaataggcta agaaggaggt cctgctgagt taattgcccc gatgtgatgg catcaatgac
     5881 tcgccccata accataggag gaatgagatt gaggacggaa accaagacca gggccacaat
     5941 cccgactaga taacggcgtt tttctaactt gaaaaaccac cagagttttt gaataatgga
     6001 cataaaatcc ctttctgatt gcaaatggaa acctgaggcc aatactcaat gaaaatcaaa
     6061 gagcaaacta ggaaactagc cgcaggctgc tcaaagcact gctttgaggt tgtagataga
     6121 actgacgaag tcagtaacct acatacggca aggcgacgtt gacgcggttt gaagagattt
     6181 tcgaagagta caagacccca ggtttttctt atttataagt tacgactgta acagcaccct
     6241 tgtcatattc agcaataaag atattggcta cattgtcatg cccttgttta ctgaggttat
     6301 caagcaacca ctcttcgcta cgaccaatcg attccaagac atctacttga atcacaccgt
     6361 cagtcacaac tggatactta ggattttcat ctcccatttg cacaacgatg agttgcccat
     6421 tttgctcttg cacagctcgt ttgacttgct tcatctggaa aatcccttgg ctacgaagtt
     6481 tgagggctac atccgatgca gacaaaccaa ctgaacgaca ggcttatggg tcaatattcc
     6541 catttttgat aaggagagtt ggcttaccat caatcaaacg tttcacaaag tgaacattgt
     6601 tgttgagcta tttgagagtc aagaccagtt tgattaaaat ttcgatataa ttaagtgtca
     6661 tttgttcaca tccaccagtt caattgcgtc tgtcttatac aattcgactt tctctaacag
     6721 gtacttgtct ggctcacttc cgtttagggc acgataatag cgttctccca ccttgataag
     6781 tgcgccatct gtgtttgtgg aagtattaac atagacttct gacttgtcta ctcccaaatc
     6841 tttggaaaca acctcgatga aatgaagtga agtttgaaat ttattgttag aaacttgatt
     6901 gttttgataa gtcgtgatgc tgaccaaaag cagagctaaa taaggccaga gctgaaatca
     6961 tgaccaactc acgaaattcg gttccctttt tatcatggta taccttaaaa gcaaaaaaac
     7021 tgtcacagct aatagaacaa tcccaaagcc aaccatgata ccattttgct gaccgatttg
     7081 gctgagtaca tagtcataag agtagaattt catgtgtttt cactcccttt cataaaatca
     7141 ttcttaatta taaacgaaag agagtaattt ttcaaactat acgttgggga aatagatctt
     7201 tttttggtat aataaaatct ataatctgaa tgaaaaaggt aactttatga aacttatctc
     7261 atggaatatt gattccctaa acgctgccct aactagtgac tcagctcgtg ccaaattgtc
     7321 ccaagaagtc ctacaaacct tggtcgctga aaatgctgat atcattgcta tccaagaaac
     7381 caagctttct gccaaaggac ctacaaagaa acacgtggaa attttagaag aactcttccc
     7441 aggctacgaa aacacgtggc gttcttccca agagcctgcc cgtaaaggct atgctggaac
     7501 catgttcctt tataagaaag aacttacacc tactatcagc ttcccagaaa tcggtgcccc
     7561 ttctaccatg gacttggaag gtcgtatcat cactctagaa tttgatgcat ttttcgtaac
     7621 ccaagtttac actccaaacg ctggtgacgg tcttaaacgc ttggaagaac gccaagtctg
     7681 ggatgtcaaa tatgcggagt atttggctga actagacaaa gaaaaaccag tccttgcgac
     7741 cggtgactac aacgtagccc acaatgaaat cgaccttgca aatcctgcta gcaaccgccg
     7801 ttcacctgga tttactgacg aggaacgtgc tggatttacc aaccttttga caactggatt
     7861 tacagacacc ttccgccatg ttcatggcga tgttcctgaa cgctacactt ggtgggcaca
     7921 acgcagcaaa acttctaaaa tcaacaatac aggctggaga atcgactact ggctcacaag
     7981 taaccgtatc gctgacaagg tcactaaatc tgatatgatt gactcaggtg ctcgccaaga
     8041 ccatacaccg attgtcttgg aaattgactt gtaaggattt tctatatgga ctacaatgcg
     8101 gtcattcccg agttgcttgt atcgaatatc gaacagtccc ggtccttcta ctgtggttta
     8161 ctgggtttta ggattgaata ccaacgtcca gaagaaaact ttctctttct cttgaagagt
     8221 gtcaactaat gttagaggag ggaacaaagg atcaactagc tgaactgacc tacccctttg
     8281 gtcgtggagt caatctctcc tttggcataa aggatgttcc aaaactctat caaaaagtga
     8341 tggaggcaaa ctatcctatt tatcgtccct tgaccaaaag aaagtttcgt gtcagtgatc
     8401 cctatatcta tcctcataaa tttgcagttt tggatccaga tggctatttt ttaagattta
     8461 gcgagtagaa taataaaaat tgggaaaagt ctttaaagtt agaaaaaggc atattgtcag
     8521 atgtctaaaa acttgatagt atgcctttta tgatggaaaa atcttctaat actcttcgaa
     8581 aatctcttca agccacgtca acgtcgcctt accgtaggta tatgatactg actctgtcaa
     8641 ttctatccac aacctcaaaa cagtgttttg aggttgtgga tagcttccta gtttgctctt
     8701 tgattttcat ttagtataaa tgttagctta caatggagtt tttgttcacc ctctcttctg
     8761 tttgttcgtt ttttaaacca aaacactaac aaaatagttg atttctaaag ttatttagtg
     8821 taaaataaaa acattggctc aagcctattt aaaattgaat atcgttttac ttgtttatgt
     8881 cgtgaattgg cacgacgttt ctacaaggtg ccggaacacc taacaataag taagtcagca
     8941 gtgagatatg gccgtttttg ccatgtctat ttaggggact tacttagaga ttaagtaggt
     9001 cttttttgaa tatttttaat atttccctta attagtgcgg acgagaggtg aaattatcct
     9061 gtgaataatt tcaggaatcc agggaatttc atccctaatt tttgagcccc tagctctttc
     9121 atttctaggc ttaggctaaa atagttcccc gaactatttt actctcaaaa atcccgtcat
     9181 agacaataac aagattattg tctatgatac cactacatcg ggaaatattt gataagactc
     9241 gcttcgctcg taatagtttt atcaaatata tagtattagc aaaagggatc actatagaaa
     9301 tatttaaaca acaagtaaat taacttttag actttaggag gtcttatgaa attattagaa
     9361 gagcgcatcc tcaaggatgg gcatatcttg ggtgataaca tcctcaaggt agattccttt
     9421 ttaacccacc aagttgactt tagcttgatg cgagagattg gtaaggtttt tgcggaaaaa
     9481 tttgctgcta ctggcattac caaggtcgta accattgaag cgtcgggtat tgccccagcc
     9541 gtttttacag ctgaagcctt aaacgttccc atgattttcg ccaaaaaagc taagaacatc
     9601 accatgaacg aaggcatctt aactgctcaa gtctactcct ttaccaaaca ggtgaccagc
     9661 accgtttcta tcgctggaaa attcctctca ccagaggaca aggttttgat tatcgacgat
     9721 ttccttgcta atggccaagc tgctaaaggc ttgattcaaa tcatcgaaca ggccggtgcc
     9781 acagtccaag ctatcggtat cgtgattgag aaatccttcc aagatggccg tgatttgctt
     9841 gaaaaggcag gctaccctgt cctatcactt gctcgtttgg atcgttttga aaatggtcag
     9901 gtcgtattta aggaggcaga tctctaatgc aaactcaaga aaaacactcg caagcagccg
     9961 ttctgggctt gcagcactta ctagccatgt actcaggatc tatcctggtt cccatcatga
    10021 ttgcgacagc ccttggctat tcagctgagc agttgaccta cctgatttct acagatatct
    10081 tcatgtgtgg ggtggcaacc ttcctccaac tccaactcaa caaatacttt gggattggac
    10141 tcccagtcgt tcttggagtt gcattccagt cggtcgctcc cttgattatg attgggcaaa
    10201 gccatggtag tggcgctatg tttggtgccc ttatcgcatc tgggatttac gtggttcttg
    10261 tttcaggcat cttctcaaaa gtagccaatc tcttcccatc tatcgtaaca ggatctgtta
    10321 ttaccacgat tggtttaacc ttgatccctg tcgctattgg aaatatggga aataacgttc
    10381 cagagccaac tggtcaaagt ctcttgcttg cagctattac tgttctgatt atcctcttga
    10441 tcaacatctt taccaaagga tttatcaagt ctatctctat tttgattggt ctggttgttg
    10501 gaactgccat tgctgctact atgggcttgg tggacttctc tcctgttgcg gtagctccac
    10561 ttgtccatgt cccaactcca ctctactttg gaatgccaac ctttgaaatc tcatctattg
    10621 tcatgatgtg tattatcgca acggtgtcta tggttgagtc aactggtgtt tatctagcct
    10681 tgtctgatat cacaaaagat ccaatcgaca gcacgcgcct tcgcaacgga taccgcgcag
    10741 aaggtttggc cgtacttctc ggaggaatct ttaacacctt cccttacacc ggattttcac
    10801 aaaacgttgg tttggttaaa ttgtcaggca tcaaaaaacg cctgccaatc tactacgcag
    10861 ctggtttcct ggttctcctt ggactgcttc ctaagtttgg tgcccttgcc caaatcattc
    10921 caagctccgt cctcggtggt gccatgctgg tgatgtttgg ttttgtatct attcaaggga
    10981 tgcaaatcct cgcccgagtt gactttgcta acaatgaaca caacttcctt atcgcagctg
    11041 tttcaatcgc tgcaggtgtc ggtctcaaca acagtaatct ctttgtcagc atgccgacag
    11101 ccttccaaat gttcttctca aacggaatcg tcgtagccag cctactcgcc attgtcctca
    11161 atgcggtact aaatcataaa aagaaataag aaaaagaggt gcgagcctct tttttgttta
    11221 tcacaaattg attataaata caatcaatcc caattcccat ctaattgaat tggacctatt
    11281 tcaaattctt taaaatatta tgttccaaat cgttaatatt atataagaat ggtaaaatat
    11341 caaatgtttc tcgtaaatta ttcttagcct tataccaacc ttgtccatca gtaatccaca
    11401 taaactcaac attaggaata gctttagttt cttcagcaat cattttgtaa gatctagccg
    11461 tctcatttaa ttttgaacca cttcctgaat aaaaattaac ctcaatcaga taaagcactt
    11521 gttcattttt tatgacaaaa tcaaaacgtt tcacagtatt cccatcattc gtaatagcag
    11581 ataaatcgac agaaaaaatt tcctctattt cattttgttc aatctcttta aataaattct
    11641 ctcctaaaat atacccttca gcttcaagat agctttgaac aatattttcc atcgcatctc
    11701 cagtccgatt tttgcgccca ttagtatcca taccaacctc aacacctgta acgtagtcaa
    11761 ctaaatttga tactaaatga ttttgcaata aatcaaatat acctgatttt tccaaaaaca
    11821 tagtatattc ttcaatacta tagtttcgtt tactaaaatc aaaatagaaa tcttttatcg
    11881 gatccttaac aataatcgta tctctcaaac gtttagcaat aagtagaggc acaactttca
    11941 aaatttctgg ataattttga tataagtcta aaaaatcctc ttgtatattt ttactaccaa
    12001 taagactatt cataatattt agagcaactt ttatactaga tacattttta taaaccttag
    12061 gaaaatctgt ataataagtc cagtcagcta ctgtgtcggt catcgttgac aaccactcat
    12121 caaaatttct agtttgtttc attttctgcc tccaacctct tccttgcaat ttttaaatat
    12181 tctttttcag catcgatacc tataaatctt ctacctaacc gcttcgcaac aacacccgta
    12241 gtgccactac caacaaatgg gtctagaata tagtcaccct ctttagtaga ggctaaaata
    12301 atacgttcta acaaatactc tggtttttga gttggatgtt tcccagccca tttttcaact
    12361 ttctttgtta aagaaccggt ccagacatct ttcatttgtt ttccatcatt caattctttc
    12421 attaaatcat aattgtagta atgacgagct tttttatcat tttttctggc ccataaaatg
    12481 gtttcagtag aatgggtaaa ataacgacaa gataaattgg gggcagggtt tgttttctgc
    12541 caagtaatat tattcagaat tttaaaacct tcttgttcta atgccattcc aactgagtat
    12601 atgttgtgca aactacctga aatccatacc gtcccattag gcttcagaac ttcttttgct
    12661 aggcgaatcc atttacgatt aaactcatgt ttttcttcga atgaagaaat tttatcccaa
    12721 tctcctttat caacagaaac tacttgtccc ccagaattag atattccacc attacttaaa
    12781 aaataaggtg gatcagcaaa aatcatatcc atactttctg gtttcatttt tgataagaac
    12841 ttgaacgtat ctgaatgaac aagaatcatc ttatttttat tgtagtatgg ttttgtcata
    12901 ag
//
LOCUS       DAVGJL010000052        12575 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.052, whole genome shotgun sequence.
ACCESSION   DAVGJL010000052 DAVGJL010000000
VERSION     DAVGJL010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12575
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.052"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            1..591
                     /locus_tag="VVE42_001187"
                     /pseudo
     CDS             1..591
                     /locus_tag="VVE42_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            782..3073
                     /gene="pcrA"
                     /locus_tag="VVE42_001188"
     CDS             782..3073
                     /gene="pcrA"
                     /locus_tag="VVE42_001188"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEU3073386.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNDLIDDVAYAAQA
                     GDMYTQIVAQCYTAYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNIWTQNADGEQIVYYRADDELDEAVFVART
                     IDELSRSQNFLHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGLGTVEKIRDFANLQNMSMLDASANIMLSGIKG
                     KAAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNAQATLESKARVENIE
                     EFLSVTKNFDDTTDVTEEETDLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDPDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSGLPFGQFTAGAKPASSEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
     gene            3116..3796
                     /gene="radC"
                     /locus_tag="VVE42_001189"
     CDS             3116..3796
                     /gene="radC"
                     /locus_tag="VVE42_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEU3073387.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQVSV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(3793..4482)
                     /locus_tag="VVE42_001190"
     CDS             complement(3793..4482)
                     /locus_tag="VVE42_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEU3073388.1"
                     /translation="MKKPVIGITGNEKTHPDDDIMMSYAAKGFVEGVKDAGGIPIILP
                     IGDQEMTCHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYHLQRDIFELALIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVKYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(4500..5141)
                     /locus_tag="VVE42_001191"
     CDS             complement(4500..5141)
                     /locus_tag="VVE42_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEU3073389.1"
                     /translation="MKDKQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGHALLH
                     YRFHERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSV
                     KSQEVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
     gene            complement(5224..5439)
                     /locus_tag="VVE42_001192"
     CDS             complement(5224..5439)
                     /locus_tag="VVE42_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEU3073390.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRGQKVYQGLVGDLYRFLSHADFLHQN"
     gene            complement(5447..5794)
                     /locus_tag="VVE42_001193"
     CDS             complement(5447..5794)
                     /locus_tag="VVE42_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEU3073391.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKELTGAKINITDKFGLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            complement(5799..6914)
                     /locus_tag="VVE42_001194"
     CDS             complement(5799..6914)
                     /locus_tag="VVE42_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU3073392.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHPEESGIDFLTTSAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEELEKQEEHFQHVQNLETAFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLEAMY
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            complement(6924..7883)
                     /locus_tag="VVE42_001195"
     CDS             complement(6924..7883)
                     /locus_tag="VVE42_001195"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEU3073393.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            complement(7996..8565)
                     /locus_tag="VVE42_001196"
     CDS             complement(7996..8565)
                     /locus_tag="VVE42_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEU3073394.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQFTGVTPVLQTNYYIDTPDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAENYLSKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDMTDYELELEVENHEQGKQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
     gene            8693..9364
                     /locus_tag="VVE42_001197"
     CDS             8693..9364
                     /locus_tag="VVE42_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEU3073395.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRGITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGAKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
     gene            9348..10166
                     /locus_tag="VVE42_001198"
     CDS             9348..10166
                     /locus_tag="VVE42_001198"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEU3073396.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLDKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIERIEYQIDHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            10163..11059
                     /locus_tag="VVE42_001199"
     CDS             10163..11059
                     /locus_tag="VVE42_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU3073397.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIYIPAEEGFETLEAIEHPLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGDGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     YIHLHTGRTHQIRVHFSHIGFPLLGDDLYGGSLEDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            11103..12077
                     /gene="pta"
                     /locus_tag="VVE42_001200"
     CDS             11103..12077
                     /gene="pta"
                     /locus_tag="VVE42_001200"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEU3073398.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
     gene            complement(12393..>12575)
                     /locus_tag="VVE42_001201"
     CDS             complement(12393..>12575)
                     /locus_tag="VVE42_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU3073399.1"
                     /translation="KIVNALQLPYSNAKLEATNNLIKLIKHNAFGFRNFENFKKRIFI
                     ALNIKKERTKFVLSRA"
BASE COUNT         3688 a         2391 c         2710 g         3786 t
ORIGIN      
        1 cgcacttatg aagaaattgc ggctgatttt ggtatccacg aaagcaactt aatccgtcgg
       61 agccaatggg ttgaagtaac tcttgttcaa agtggtgtta cgatttcaag aactcctctc
      121 agctctgagg acacggtaat gattgatgcg acggaagtaa aaatcaatcg ccctaaaaaa
      181 acaattagcg aatcattctg gtaaaaagaa atttcacgct atgaaggctc aagcgattgt
      241 cacaagtcaa gggagaattg tttctattgt catgatatga agttgttcaa aatgagtcgc
      301 agaaatatcg gacaagctgg taaaatcttg gctgacagtg gttatcaagg gctcatgaag
      361 atatatcctc aagcacaaac tccacgtaaa tccagcaaac tcaagccact aatagctgaa
      421 gataaagctt ataaccatgc gctatctaag gagagaagca aggttgagaa catctttgcc
      481 aaagtaaaaa cgtttaaaat gatttcaaca atctatcgaa atcatcataa acgcttcgga
      541 ttacgaatga atttgagtgc tggtattatc aatcatgaac taggattcta gttttgcagg
      601 aagtctattg aggtattgag ctagtttatg aaaaaattgg gtgaaaagtc gagtgtttta
      661 gaaacccaca gtgtagtatt ctagtttcaa tccactatat tttgctactc cccataaagt
      721 ttctattttc cctgatttct gatataatag aaatattgac ttcaagagta aggaagagaa
      781 gatgaacgca ttattaaatg gaatgaatga ccgtcaggct gaggcggtgc aaacgacaga
      841 aggtcccttg ctaatcatgg caggggctgg ttctggaaag actcgtgttt taacccaccg
      901 tatcgcttat ttgattgatg aaaagctggt caatccttgg aatatcttgg ccattacctt
      961 taccaacaag gctgcgcgtg agatgaaaga gcgtgcttat agcctcaatc cagctactca
     1021 ggactgtctg attgcgacct tccactccat gtgtgtgcgt attttgcgtc gcgatgcgga
     1081 ccatattggc tacaatcgta attttacaat tgtggatcct ggtgaacagc gaacgctcat
     1141 gaaacgtatt ctaaaacagt tgaacttgga tcctaaaaaa tggaatgaac gaactatttt
     1201 ggggaccatt tccaatgcta agaatgattt gattgatgat gttgcttatg ctgcccaagc
     1261 tggcgatatg tatacgcaaa ttgtggccca gtgttataca gcctatcaaa aagaacttcg
     1321 tcagtccgaa tccgttgact ttgatgattt gattatgctg accttgcgtc tctttgatca
     1381 aaatcctgat gttttgacct actaccagca aaaattccaa tacatccacg ttgatgagta
     1441 ccaagatacc aaccacgctc agtaccaatt ggtcaaactc ttggcttccc gttttaaaaa
     1501 tatctgtgtg gttggggatg cggaccagtc tatctacggt tggcgtggtg ctgatatgca
     1561 gaatatcttg gactttgaaa aggattaccc caaagccaag gttgttttgt tggaggaaaa
     1621 ttaccgctca accaaaacca ttctccaagc ggccaacgag gttattaaaa ataataaaaa
     1681 tcgccgtcct aaaaatatct ggactcaaaa cgctgatggg gagcaaatcg tttactatcg
     1741 tgccgatgat gagctggatg aggctgtatt tgtagccaga accatcgatg aacttagtcg
     1801 cagtcaaaac ttccttcata aggattttgc agttctctat cggactaatg cccagtcccg
     1861 tacaattgag gaagccctgc tcaagtctaa cattccttat accatggttg gcggaaccaa
     1921 attctacagc cgtaaggaaa ttcgcgatat tattgcttat ctcaacctta ttgctaattt
     1981 gagtgacaat attagttttg agcgtattat caacgagcct aaacgtggaa ttggtctagg
     2041 tacagttgag aaaatccgtg attttgcaaa tttgcaaaat atgtctatgc tggatgcttc
     2101 tgctaatatt atgttgtctg gtatcaaggg taaggcagcc caatctatct gggattttgc
     2161 caatatgatg cttgatttgc gggagcagct agaccactta agcattacag agttggttga
     2221 gtccgtccta gaaaaaacag gttatgtcga tattcttaac gcccaagcga ctctagaaag
     2281 caaggcacgg gttgaaaata tcgaagagtt tctttctgtt acgaagaact ttgatgacac
     2341 cacggatgtg acagaagagg aaactgatct ggacaaactg agtcgtttct taaatgactt
     2401 ggctttgatt gccgacacag attcaggtag tcaggagaca tcagaagtga ccttgatgac
     2461 cctgcatgct gccaaaggtc tcgaatttcc agttgtcttt ttgattggga tggaagaaaa
     2521 tgtctttcca cttagtcgtg cgactgaaga tccagatgaa ttagaagaag agcgccgtct
     2581 agcctatgta ggtatcacgc gtgcagagaa aattctctat ctgaccaatg ccaactcacg
     2641 cttgcttttt ggtcgtacca attataaccg tccgactcgt tttattaacg aaatcagttc
     2701 agacttgctt gagtatcaag gtctggctcg tcctgcaaat acaagcttta aggcatcata
     2761 tagcagtggt agtatttcct ttggtcaagg tatgagtttg gctcaggctc ttcaagaccg
     2821 taaacgcggt gctgccccaa aatcaatcca gtcaagcggt cttccatttg gtcaatttac
     2881 agctggcgca aaaccagcat ctagcgaggc aaattggtcc attggtgata ttgctctcca
     2941 caagaaatgg ggagagggaa ccgttctgga agtttcaggt agcggtgcta ggcaggaatt
     3001 gaaaatcaat ttcccagaag taggtttgaa aaaactttta gccagtgtgg ctccaattga
     3061 gaaaaaaatc taattttcca tccttctcac gaataataaa gtgaggagga tttttatgta
     3121 cagtatttca ttccaagaag attcactatt accaagagaa aggctggcca aggaaggagt
     3181 tgaagcgctt agtaaccaag agttgctagc tattttactc aggacaggaa cacgtcaagt
     3241 tagcgttttt gaaattgccc aaaaagtctt gaacaatctt tcaagcctaa cggatttgaa
     3301 aaaaatgacc ctgcaggaat tgcagagttt gtctggtatt gggcgtgtta aggccataga
     3361 attacaagct atgattgaac tggggcatcg tattcacaaa cacgagactc ttgaaatgga
     3421 aagtattctc agcagtcaaa agttggccaa gaagatgcag caggaattag gggataaaaa
     3481 acaagagcac ctggtggcac tctatctcaa tactcaaaat caaatcatcc atcagcagac
     3541 catttttatc ggttctgtaa ctcgtagtat cgctgaaccg cgagagattc ttcactatgc
     3601 aatcaagcat atggcgactt ctcttatctt ggtccacaat catccttcag gagcggtagc
     3661 gcctagccaa aatgatgatc atgtcactaa acttgttaaa gaagcctgcg aactgatggg
     3721 gattgttctc ttggaccatt tgattgtctc tcattctaat tactttagtt atcgtgaaaa
     3781 gacagattta atctaaagtt cattaacgac atagtcaaag agtttcttat ctttgggacg
     3841 attttcaaaa agaagttctg gatgccattg gacaccgaga aaggcgacat catccgtact
     3901 catgacagcc tcaatgatac catctttagg atcatgagcc acaactttta aatttggtgc
     3961 taagtccttg atgctctggt ggtggaagga gttgatatga gagatttctc catagatttc
     4021 ttggagaacg gtatctggtt ctgtcaccaa gcgttgagtt gtgtacttaa cagaagaatc
     4081 ctgccaatgg tcttcgatat cttggtacaa agttccaccc atggcaacgt taaagagttg
     4141 ggtaccacgg cagacagaga aaatgggctt tttctgttta atagcttcct tgatgagggc
     4201 cagttcgaag atatctcttt gaaggtgata gtcatcgcta tcaatggttt tgggttcgcc
     4261 ataaaatttt ggatcgacat tttgcccacc tgttaagatg agcttgtcaa tcaaactgat
     4321 atagtggcag gtcatttctt gatcaccaat cggtaggatg atgggaatcc ctccagcatc
     4381 tttaacgcct tcaacaaagc cttttgctgc gtagctcatc atgatgtcat catctggatg
     4441 agttttttcg tttcctgtaa tcccaataac tggttttttc ataaaatgat tttcgctttc
     4501 taatcctctt ttcgcatgaa gtagaggagg gtttggagtt cacttgtcaa atcgacatac
     4561 tgaacgacca cgtcttttgg taaatgcaga tggactggtg aaaaactgag aattcctttc
     4621 acaccagcat caaccaagag attagcaacc tcttgtgact tgacgctggg aacagttagg
     4681 atagcagtct tcacatcagc atccttgatt ttatccttga tctgagaaat cccgtaaatg
     4741 ggaatcccgt caggagtttg ggtaccgact tcaggatggt cgtctaggtc aaaggccatg
     4801 ataatcttca tcttgttacg ttcgtggaag cggtagtgga gaagggcatg gcccatattt
     4861 ccaataccaa ccagcatgac attggtaata gagttgtcat tgagcaaatc ggcaaaaaat
     4921 gtcattagtt ttttgacatc atagccaaaa ccacgacgac caagttcacc aaaataggaa
     4981 aaatcacgac gtacggtcgc tgaatcaatc ccaatggcct ctgcaatttg cttagagttg
     5041 gcacgttcaa tcttttctgc atgaaatctc ttaaaaattc gatagtagag agagagtctt
     5101 tttgctgtag cttttggaat agcaaactgt ttatctttca caaaatcaca acctttctat
     5161 tcttctattt tatagaaaca ttgtgaaaaa atcaacaaaa ataagaaaaa actaagaaaa
     5221 atcttagttt tgatgtaaaa aatctgcatg agatagaaaa cggtagaggt ctccgaccag
     5281 accctgataa actttttggc ccctaaaagt cagagaagtc acataaagtg tatctggtaa
     5341 ggttacacat cctgacaaag tcaacatgag agcctcatga tcctcatact tgagagtacg
     5401 ctctacatga tagcagtcct tataggtcag ttcaaacatt ttggctctat ctttccgatt
     5461 ttgtaaagac accacgttct accaagctat ccatgaggaa gtagaatttt tcctgatgaa
     5521 tatggtggtc ttctgatttg aaaatatcaa ctagacgaag gccaaacttg tcagtgatat
     5581 tgattttagc ccctgtaagt tccttgttaa tgatgatttt gagttggaag ccttcaccgc
     5641 tgtttggcac tttttccaaa aggcgagtca gttcatagtt accaacctta gtttcaaaaa
     5701 aggtgttatc tttgagggtg aattttttaa cagaagggct aagagtgtaa tcgtaacgac
     5761 aattttttaa ctgaatgatt ttttcaaatg ccatatggct aacctccgat aatttctttt
     5821 aaggtttttg cgagggtttg taggtcttca acggtatttt gtggcgacaa actgatgcga
     5881 agggattcct tcaagcgttc tgaatttgcg ccatacatgg cttcaagaac atggctggat
     5941 tggacaacgc ctgcagtaca ggctgagcca gtagagattg aaattccagc taaatctagc
     6001 cgaaggagta agaggtcatt tttctgacca ggaaatccaa tattgagaac ataagggaga
     6061 tgatgttttc ctctattcaa gtaatactga atgccctcca gctctgccag aaaggcagtt
     6121 tctagatttt gtacatgttg aaaatgttct tcttgttttt ctagttcttc ttttagggct
     6181 gcaaccatgc ctacaatggc aggcagattt tcagttcctg cacgtttttt ctgttcctgg
     6241 tctccgccat gtagatagga atcaaagtcc atgctagatg cgtagagaaa accgattccc
     6301 ttaggaccat ggaatttgtg ggcagaagta gtgagaaaat caatgcccga ttcttctgga
     6361 tgaataggga ttttaccaat agcctgaact gcatcgacat gataggcggc tgggtgttgc
     6421 ttgagtattt ggccaatttc agcgatgggc agtaggtttc ctgtctcatt attggcaaac
     6481 atggtagaaa ccaaaatcgt atcgtcacgt aaagcctttt gaatttgctg ggctgtgatt
     6541 tcttgatttt ctggctggat aatggttgct tcaaacccaa agtgttgaac caagtaatca
     6601 attgtttcaa ggacagcatg gtgctcgatg gcagttgtga tgatatgttt tccttgttct
     6661 tggtgacgaa gacagtagcc aatgatggta gtattattgc cttcagtccc accagaagtg
     6721 aaaaagatat gttgaggttt tgtccttagt aactgggcta gttcctgacg ggcttctcgc
     6781 aagagtttgc cagcttgacg accatgacca tgaatactag aaggatttcc gtgggtttct
     6841 tgcataacct tggtcatagc tgaaatagca actgctgaca taggagtcgt tgcagcattg
     6901 tccaaataaa tcaaagaatc accttatttc tttttattgt aggcaaagag tgggctgact
     6961 ggttttcttt cgtgaatacg gacgatagca tcaccaatta actcactagc agtgatgtag
     7021 catacatttt taggagtttt ttcttttgtt gctactgaat cagtcacaag aatttcttta
     7081 atattagtat tgtcaagaag ctcagcagct ccctcgacga agagaccgtg gctagaaaca
     7141 gcataaattt ctgtagctcc ttcacgttca acgattttag aagcttcaga gaaggtacgt
     7201 cctgtattta aaatatcatc aatcaagata gctttcttac cttcaacatc accaataata
     7261 taaccttcgt tacgagttgc atcgtcttga gggtagtcga taatggcgat aggagcatca
     7321 agatattcag ccaggctacg cgcacgtttg acacctgaat ttttagggct aacgacaaca
     7381 acatctgaac caagcaatcc tttatcgcag taatgttttg cgaatagggg aacagtgaaa
     7441 agattatcca ctggaatatc aaaaaaacct tgaacctgaa cggcatgtaa atcaagagtc
     7501 aggatacgat caactccagc cttaaccagc atattggcaa ctagttttgc tgtaagtggc
     7561 tcacgaggac aagcaatgcg gtcttgacgt gcatagccaa aatatggaag gacaacgttg
     7621 atactgtggg cacttgcacg cacacaagca tcgaccatga ttaacagttc cattaggtgg
     7681 ttgttgacag ggaaacttgt tgattggatg atgtaaacat cataaccacg gacactttct
     7741 tcgatattta cttggatttc tccgtctgaa aattgacgtg atgatagttt tccaagtggg
     7801 acaccaacag cttgggcaat tttctgtgca atctcttggt tagagttaag tgcgaaaagt
     7861 ttcatgtttt ttctatctga cattatagac cgtcctctgt aaactttata aatcctagtt
     7921 atatttatct tacatatatg aactagggtt tgtgtatttt tatcttttct attttaccaa
     7981 aaaatggaga ttatttcagc tatttttcat acttttgaca aatcgaacca attttgaagg
     8041 agctttttgg taggaaatct gatttttctc taaaaattgt cggaaatcct gtttgccttg
     8101 ctcatgattt tccacttcaa gctccaattc gtaatctgtc atatcaaagt atcggctctg
     8161 atccagtgcc atgagaccaa tagctgtttt catttcatag cgaagcgttg ttagacaacc
     8221 aagaacctgc cagttcttac tttggatacc atgtttcgcc aattcatcca gtactagtcc
     8281 ttgaggaagt tcttccttac tcagatagtt ctcagcatct tttagttgca atttttggtt
     8341 gtattccatg tttccaacac tctgcgggac tttgagtgtc aactcagccc agtcttcaaa
     8401 ggttcgaatg cgcatagcga ctttcttttc tcgcagttca aaatcaggcg tgtctatgta
     8461 gtaatttgtt tgaagaacag gagtgacacc tgtgaactgg tcttttagac gattgtattc
     8521 atcttttttc aatagtgttt tcaattcaat ttctaaatgt ttcatttttc ttaccttttt
     8581 ttatcgttga aagcggattt atggtataat aagcattgta tttattgtat atgaatctgg
     8641 agaaaaaatc aaagatattt ttgacggata atatgagaac aagggagaat atatgacctt
     8701 agaatgggaa gaatttctag atccttacat tcaagctgtt ggtgagttaa agattaaact
     8761 tcgtggtatt cgtaagcaat atcgtaagca aaataagcat tctccaattg agtttgtgac
     8821 cggtcgagtc aagccaattg agagcatcaa agaaaaaatg gctcgtcgtg gcattactta
     8881 tgcgaccttg gaacacgatt tgcaggatat tgctggctta cgtgtgatgg ttcagtttgt
     8941 agatgacgtc aaggaagtag tggatatttt gcacaagcgt caggatatgc gaatcataca
     9001 ggagcgagat tacattactc atagaaaagc atcaggctat cgttcctatc atgtggtagt
     9061 agaatatacg gttgatacca tcaatggagc taagactatt ttggcagaaa ttcaaattcg
     9121 tactttggcc atgaatttct gggcaacgat agaacattct ctcaactaca agtaccaagg
     9181 ggatttccca gatgagatta agaagcgact ggaaattaca gctagaatcg cccatcagtt
     9241 ggatgaagaa atgggtgaaa ttcgtgatga tatccaagaa gcccaggcac tttttgatcc
     9301 tttgagtaga aaattaaatg acggtgtagg aaacagtgac gatacagatg aagaatacag
     9361 gtaaacgaat tgatctgata gccaatagaa aaccgcagag tcaaagggtt ttgtatgaat
     9421 tgcgagatcg tttgaagaga aatcagttta tactcaatga taccaatccg gatattgtca
     9481 tttccattgg cggggatggt atgctcttgt cggcctttca taagtacgaa aatcagcttg
     9541 acaaggtccg ctttatcggt cttcatactg gacatttggg cttctataca gattatcgtg
     9601 attttgagtt ggacaagcta gtgactaatt tgcagctaga tactggggca agggtttctt
     9661 accctgttct gaatgtgaag gtctttcttg aaaatggtga agttaagatt ttcagagcac
     9721 tcaacgaagc cagcatccgc aggtctgatc gaaccatggt ggcagatatt gtaataaatg
     9781 gtgttccctt tgaacgtttt cgtggagacg ggctaacagt ttcgacaccg actggtagta
     9841 ctgcctataa caagtctctt ggcggtgctg ttttacaccc taccattgaa gctttgcaat
     9901 taacggaaat tgccagcctt aataatcgtg tttatcgaac actgggctct tccattattg
     9961 tgcctaagaa ggataagatt gaacttattc caacaagaaa cgattatcat actatttcgg
    10021 ttgacaatag cgtttattct ttccgtaata ttgagcgtat tgagtatcaa atcgaccatc
    10081 ataagattca ctttgtcgcg actcctagcc ataccagttt ctggaaccgt gttaaggatg
    10141 cctttatcgg tgaggtggat gaatgaggtt tgaatttatc gcagatgaac atgtcaaggt
    10201 taagaccttt ttaaaaaagc acgaggtttc taagggattg ctggctaaga ttaagtttcg
    10261 aggtggagct attctggtca ataatcaacc gcaaaatgca acgtatctat tggacgttgg
    10321 agactacgtt accatttaca ttcccgctga ggaaggcttt gaaaccttgg aggctattga
    10381 gcatccatta gatattctct atgaggatga ccactttcta gtcttgaata aaccctatgg
    10441 agtggcttct attcctagtg tcaatcactc taataccatt gccaatttta tcaagggtta
    10501 ctatgtcaag caaaattatg aaaatcagca ggttcacatt gttaccagac tagataggga
    10561 cacttctggc ttgatgctct ttgccaagca cggttatgcc catgcacgat tagacaagca
    10621 gttgcagaag aaatctatcg agaaacgcta ctttgctttg gttaagggag atggacattt
    10681 ggagccagaa ggggaaatta ttgctccgat tgcgcgtgat gaagattcca ttattaccag
    10741 acgagtggct aaaggcggaa agtatgccca tacttcatac aagattgtag cttcttatgg
    10801 aaatattcac ttggtctata ttcacctgca cactggtcga acccatcaaa tccgagtcca
    10861 tttttctcat atcggttttc ctttgctggg agatgatttg tatggtggta gtctggaaga
    10921 tggtattcaa cgtcaggctc tgcattgcca ttacctatcc ttttatcatc catttttaga
    10981 gcaagacttg cagttagaaa gtcccttgcc ggatgatttc agtaacctta ttacccagtt
    11041 atcaactaat actctataaa aactgtctca gagtataatt attatcttaa aggagaaaac
    11101 tcatggaagt ttttgaaagt ctcaaagcca accttgttgg taaaaatgct cgtatcgttc
    11161 tccctgaagg ggaagagcct cgtattcttc aagcaacaaa acgcttagta aaagaaacag
    11221 aagtgattcc tgttttgctt ggaaatcctg aaaaaattaa aatttatctt gaaattgaag
    11281 gaatcatgga tggttatgag gtcatcgacc ctcaacatta tcctcaattt gaagaaatgg
    11341 tttctgcctt ggtggagcgt cgcaagggca aaatgactga agaagatgta cgcaaggttt
    11401 tggttgaaga tgtcaactac tttggtgtga tgttggttta cttgggcttg gttgatggaa
    11461 tggtgtcagg agcgattcac tcaacagctt caacagttcg tccagctcta caaatcatca
    11521 aaactcgtcc aaatgtaact cgtacttcag gagccttcct catggttcgt ggtacggaac
    11581 gttacctatt tggagactgt gccattaaca tcaatccaga tgcagaagcc ttggctgaaa
    11641 ttgccatcaa ctcagcaatc acagctaaga tgtttggcat cgaacctaaa attgccatgt
    11701 tgagctattc tactaaaggt tcagggtttg gtgaaagcgt tgataaggtc gttgaagcaa
    11761 ctaaaattgc tcacgacttg cgtcctgacc ttgaaatcga tggtgagttg caatttgatg
    11821 cagcctttgt tcctgaaact gcagctctga aagctcctgg aagtacggta gctggtcaag
    11881 caaatgtctt catcttccca ggtatcgagg caggaaatat cggttacaag atggctgaac
    11941 gcctgggtgg ctttgcggct gtaggacctg ttttgcaagg tttaaacaag ccagttaatg
    12001 atctttctcg tggatgtaat gcagatgatg tttacaagtt gaccctcatc acagcagctc
    12061 aagcagttca tcaatagtga aaactataaa gcgatatact atgctatact gtagttatga
    12121 aactatgtac gaaaagcact gccattaatt cctgagaact aaattactga ttggtgtcaa
    12181 aaaggaaaac ttccaagcga tgatatcctg tctatacacg acctatagaa atctgtaata
    12241 tacatgtccg taaaacgata aattcccttt ttgattttaa atgagtatga aaagagaatt
    12301 ttttcatgag aaaacgcata atattaagtg tcttgacacc tgctattatg cgttttctgg
    12361 ctctttgtca actgtagtgg gttgaagaaa agctaagctc gagaaaggac aaattttgtc
    12421 ctttcttttt tgatgttcag agcgataaaa atccgttttt tgaagttttc aaagtttcga
    12481 aaaccaaagg cattgtgctt gataagtttg atgagattat tggtggcttc cagtttggcg
    12541 ttggaataag gtaattgaag ggcgttgacg atttt
//
LOCUS       DAVGJL010000053        12476 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.053, whole genome shotgun sequence.
ACCESSION   DAVGJL010000053 DAVGJL010000000
VERSION     DAVGJL010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12476
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.053"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(112..459)
                     /locus_tag="VVE42_001202"
     CDS             complement(112..459)
                     /locus_tag="VVE42_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU3073400.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKADYANDGFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEVEDVDQNYKRLNELGIKVLHGPTVTDWGTESLLVQ
                     GPAGLVLDFYRMK"
     gene            complement(570..2345)
                     /gene="spxB"
                     /locus_tag="VVE42_001203"
     CDS             complement(570..2345)
                     /gene="spxB"
                     /locus_tag="VVE42_001203"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEU3073401.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAVSKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
     gene            complement(2552..4795)
                     /locus_tag="VVE42_001204"
     CDS             complement(2552..4795)
                     /locus_tag="VVE42_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU3073402.1"
                     /translation="MTEIVKANLENGIQKIRIRAEKGYHPAHIQLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEAIGVDEEKVIRFTPQELGRHEFSCGMKMQKGSYTVVEKTR
                     KSLSLLQRFWITSIFTVPLVILMIGMLTGSISHQVMHWGTFLATTPIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDIIGDEVEVFGLAASLEDASQHPLAEAIVKRASEAGLEFQTVENFQALHGKGV
                     SGRINGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKENAKLAISQLKKRGLRTVMLTGDNADVARAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QQSGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLSLNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            complement(4805..5176)
                     /locus_tag="VVE42_001205"
     CDS             complement(4805..5176)
                     /locus_tag="VVE42_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEU3073403.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKKPEKSEQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHANLPMGEEYVVEITPE
                     QAGEFGFACGMNMMHGKMIVE"
     gene            complement(5187..5582)
                     /locus_tag="VVE42_001206"
     CDS             complement(5187..5582)
                     /locus_tag="VVE42_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEU3073404.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTVQTLL
                     ARLVEKECLTRKKEGKSFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     regulatory      5766..5864
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5948..6739
                     /gene="thiD"
                     /locus_tag="VVE42_001207"
     CDS             5948..6739
                     /gene="thiD"
                     /locus_tag="VVE42_001207"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEU3073405.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQARDVYGMAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLDSVFSDIPPQAVKTGMLATTEIMEIIQPYLKKLDCPY
                     VLDPVMVATSGDALIDSNARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEDMQRA
                     GRLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVI
                     TAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
     gene            complement(6949..7581)
                     /locus_tag="VVE42_001208"
     CDS             complement(6949..7581)
                     /locus_tag="VVE42_001208"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEU3073406.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKIETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARQVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPV
                     VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLTKLHDILS"
     gene            complement(7574..8377)
                     /locus_tag="VVE42_001209"
     CDS             complement(7574..8377)
                     /locus_tag="VVE42_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEU3073407.1"
                     /translation="MQALTNPFPIGSSSLIHCMTNEISCEMLANGILALGCKPVMADD
                     PREVLDFTKQSQALFINLGHLSAEKEKAIRIAASYAAQVCLPMVVDAVGVTASSIRKS
                     LVRDLLDYRPTVLKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYPG
                     MSFLVTGPKDLVVSKNQVAVLGNGCTELDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEVYE"
     gene            complement(8377..8901)
                     /gene="thiW"
                     /locus_tag="VVE42_001210"
     CDS             complement(8377..8901)
                     /gene="thiW"
                     /locus_tag="VVE42_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HEU3073408.1"
                     /translation="MRKHQLQVHKLTILSMMIALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFYFSALGE
                     IVGTGIIGSIVSYPVMVLFTGSAAKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFAERIE"
     gene            complement(8906..9598)
                     /gene="tenA"
                     /locus_tag="VVE42_001211"
     CDS             complement(8906..9598)
                     /gene="tenA"
                     /locus_tag="VVE42_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEU3073409.1"
                     /translation="MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKAFSEIYHLLADKTSNQEMKRLLKQNAQGLVEGELFIRQQFFKELEINDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDAFHISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            complement(9609..10259)
                     /locus_tag="VVE42_001212"
     CDS             complement(9609..10259)
                     /locus_tag="VVE42_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ABC transporter permease"
                     /protein_id="HEU3073410.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALNLSVVAFCILFMLY
                     YRRFKMLAWMIILAILPSFANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSISLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQELHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAHGYDEHAQLKNSYRTFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            complement(10261..11646)
                     /locus_tag="VVE42_001213"
     CDS             complement(10261..11646)
                     /locus_tag="VVE42_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3073411.1"
                     /translation="MGLELRAIQSPIFSEPFDFTFHAQAFTLLVGSNGSGKSSLFQMI
                     AQVSSLPYSGQVLIDGSEVSQLSIIARVQKVGILLQNPNHQFTMENLFEELIFTLENI
                     GYHLQEIDSKIAEVVQQCRCEAILHRPIHHLSGGEKQKAALAVLFAMNPRVYLLDEPF
                     ASIDRKSRIEILEILKELALDGKTVILCDHDLSDYKAYIDYMVELRDGKLREVFQIPS
                     YEMTQVASKEVAASPELFHMNRVTGELGNRPLFSIADFTFYQGISCILGDNGVGKSTL
                     FRSILQFQKYKGRIAWKGTVLKKKKSLYRDLTGVVQEAEKQFIRVSLREELQLDGPDS
                     ERNQRIFQALRYFDLEQAVDKSPYQLSGGQQKILQLLTILTSKASVILLDEPFAGLDD
                     RACHYFCKWIVEERNQGRSFLLISHRLDPLISVVDYWIEMTSQGLRHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            complement(11647..12207)
                     /locus_tag="VVE42_001214"
     CDS             complement(11647..12207)
                     /locus_tag="VVE42_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU3073412.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNILSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGLVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIKLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
BASE COUNT         3751 a         2779 c         2385 g         3561 t
ORIGIN      
        1 atatgcaatt tctttatata aaaagaccgg attgctccga tctttcaata gttcatattc
       61 tcaatttcta ttttaaaaat agctaaggtt aacgtcaaat gactacgcga cctatttcat
      121 acgataaaaa tcaagcacta gaccagcagg tccttgaact aataaggact ctgttcccca
      181 atcggttaca gttggtccgt gtaaaacctt tataccaagc tcgttcaacc gtttgtagtt
      241 ctggtctaca tcctcaacct cgatatgaat aatgattcct gactgaaagt tttccaaagg
      301 aaccaaatga ttttgtgaca acataaggca gtgactacca atcgtaaact gagcaaaacc
      361 atcattagca taatctgcct ttttatccaa gatatgctcc aagtcagcac agacttgggg
      421 aacatttgaa acgataatat ctaattgatt taaattcatt tactctcctc cataaaaaga
      481 ccggattgct ccgatctttt aaagttctgc tctatgaaaa tcaaagaata aagtctacaa
      541 gtttcatatt tgattttcgg cgagaggaat tatttaattg cgcgtgattg caatccttct
      601 tcttccaaga agagacggaa tggtacgagt tcttctgctt cgtatttttc cttgaaggct
      661 ttgatagctt cttctgagtg aagttttgga tccaattcaa gtacttctac tggaagtgga
      721 cggtgttgag tgatgcgagc atcgatgaca acagttttac cttctttgtt caatttaaca
      781 gcttctgcaa caactgcatc gatgtcttcg atacggtcaa ctgtgaatcc aacagctcct
      841 tgagcttccg caattttagc gtagtcagcg tttgtgaagt ctacaccaaa caagtgtttg
      901 tttgtatctt cgtatttgtt cttgatgaag ccgtactcag catttgagaa gacaaggttg
      961 ataactggaa ggtcgtattg aacgtttgtg ataacgtctg ggtagcacat gttgaatgct
     1021 ccgtcaccca tgatgttcca tacttggcga tctggattgt ctttcttagc agcgatacca
     1081 ccaggaaggg caatacccat tgtcgcaaag agtggagatg tacgccacat gttcttaggt
     1141 gtcatgtgaa ggtgacgagt agatgtttga gtagtgttac ctacgtcgat tgagtagata
     1201 gcgtcttgat cagcatgttt gttgattgca ttgtaaactt gatacaattg caattcaccc
     1261 tcagttttac cttcgagttt gttcatgtaa tcacgccagt tttggttgtt cttaacgttt
     1321 gcacgccacc atggagttga ttcaactggg tttactttgt caaggatagc tttagctgct
     1381 tgaccagcat caccaaggat tgaagcgtca agggcatgac gtttaccaag tttgtaaggg
     1441 tcgatatcga cttggatgaa tttttcagtg ttcttgaatg cttcgtaaac ttcagcaaat
     1501 gggaagtttg aaccaaggaa aagaactgtg tctgcttcaa agaccacttc gttggctggt
     1561 ttccaaccaa cacggtaagc agaacctgtc aaaccttcat agttccattc gaaagcttca
     1621 aagtttttac cagttgtgat gattggtgct ttgattttac gtgacaattc agtaatcact
     1681 tcaccagctt taacaccacc aaatccagca tagataactg ggcgttcagc attgttcaag
     1741 atttcaacag ctttgtcgat ttcaacttcg ttcaaagcag gagcgatgaa tgagcgttcg
     1801 tatgaacctg aaccgtagta tgagttttca tcgatttctt ggaaaccgaa gtttactgga
     1861 atttcaacaa cagctggacc ttttttagaa actgcagcac ggcaggcttc gtcaattact
     1921 tttggcaatt gctcagcgta agctacacgt ttgttgtaaa cagcgatacc gttgtacatt
     1981 gggttttgat taagctcttg gaaagcatcc atgttcaatt cgttaactgg acgtgatcca
     2041 aggatcgcta ggaatggagt gttatccata gctgcatcgt aaacaccgtt aatcaagtga
     2101 gtcgcacctg gaccacctga accaactgca accccgattg agccgccgaa tttagcttgc
     2161 ataaccgctg caagagcacc tgtctcttcg tggcgaactt gtaagaaacg gatatctttg
     2221 tcttcagcca aagcatccat caatgagctg agtgttcctg atgggatacc gtagattgta
     2281 tctacgcccc atgttttcaa tacgttaagc attgctgcag atgcagtaat tttcccttga
     2341 gtcataatga taactctcct tcaatttttt taaacttgga gaatacgatt acatagaatt
     2401 ggaaacgttc tccaaatttt tactattcca ctgtatcata tttatgctga cttttctaaa
     2461 aatctgctca aaactctcta ttctctattc taatacagtt ttgaaagtgt tgtcatttct
     2521 gttttataac aaagaaatct agtcattact tttagtctat tttactaaaa tttaacagaa
     2581 gggaactggt cagaacagat acagaactaa aggccatggc tagacctgcc aattctgggt
     2641 tgagagacag tccaacacct gaaaagactc ctgctgcaat cggaattccg acaacattgt
     2701 agataaaagc ccagaaaaga ttgagtagaa ttcgatgaaa ggttttctta ctcatatcaa
     2761 aggcacgaac cactcctaaa agattattgg ttgtcaacac caaatctgct gactcgatgg
     2821 cgatatctgt tccagctccc atagcaatcc ccacatctgc tacactaagg gcaggagcgt
     2881 cattgatacc gtccccaaca aaggctactt tccctgactg ttgcagttta tggatttcat
     2941 gggctttttc ttctggcaag acgcctgcaa tgacctcttc aattccgatt tgatctgcaa
     3001 tagcacgcgc cacatcagca ttgtctcctg tcagcatgac tgttcggaga ccacgttttt
     3061 ttagctgact gatggctagc ttagcatttt ccttaggaat atcttgcaaa gcaagcaagc
     3121 ctttgatttc attgtcaaca gctaaaaaca caactgtctt agcttctttt tctagttctt
     3181 ctagtttatc ttgataagta ttagaaatat ccatgccatc cagcatttta gcatttccaa
     3241 gtaaaacttg ttttccattg attcgccctg aaacaccttt cccgtgcaag gcctgaaaat
     3301 tttcaacagt ttgaaactca agtccagctt cactcgctcg cttaacgata gcctcagcca
     3361 gtgggtgttg agaagcatct tccaaggagg ctgccaatcc aaacacttct acttcgtcgc
     3421 cgatgatatc tgttaccaca ggtttccctt ccgtcaaagt cccggtctta tcaaagacaa
     3481 gagtttgaac tttctggatt tcctgtaaga cagttccatt tttgaggaga acccccatct
     3541 tggcactacg tcctgtcccc accataaggg ctgtcggtgt tgcaagtccc aaggcacaag
     3601 gacaggcgat aatcaaaacc gccactccgt agagaagaga ggacacaaag ctagctccaa
     3661 gcacgaccac actatccctg agcaagacga accaaaccca aaaggtcatg attcctaaaa
     3721 tgacaactac tgggacaaaa atccctgaaa tcttatccgt caagtcctga atcggcgcac
     3781 gacttgtctg agctttcttc acaaaatcca caatctgagc caaaacagtc tctgagccaa
     3841 ctttttctgc tctaaagaca agcgttccac tatgattgat ggttgagcca atgacagtat
     3901 ctccaactgt cttgtccaca ggcagactct cacctgtcac catggattcg tcaatactag
     3961 agacaccttc tactacgaca ccatcaacag caatcttttc accgggacgc actcgaatca
     4021 ggtcgcctac cttgacttgt tccaaaggaa cttggacata actatcatca ctcaagactt
     4081 ctgcggtttt agcttgcaag tccagtaatt tctccacagc ttgggacgta ttttttctca
     4141 ttttttcctc aaaaactgct cccaaaagaa cgaaaaagag gataaatcca gcactttcga
     4201 agtaaacagg gagaccagca aagagagcaa ctaggctata gaaataagcc actagagttc
     4261 ccagcgcaac caaggtatcc atgttggcat tgtgcttttt aaaactggcc caagcactct
     4321 ggatatatgg cttacctgca actaacataa taggcgttgt tgctagaaag gttccccaat
     4381 gcatgacttg atgactaatg ctacctgtca acatcccaat catgagaatc acaagaggca
     4441 cagtaaagat actagtaatc caaaaacgtt gcaggagaga tagagatttt cgagtcttct
     4501 caacgactgt atagcttccc ttttgcatct tcatgccaca agaaaattca tgtcgcccta
     4561 attcttgagg cgtaaaacga atgactttct cctcatctac gccgattgct tccaagatac
     4621 cttcttcttc aaacagaatt tccttataac agtttgaagg agtagcacga tgaaaggtaa
     4681 tctcagctgg aattcccttt tgaagctgga tatgggctgg atgatagcct ttttcagctc
     4741 ggatacggat tttttgaatg ccattttcta agtttgcttt cacaatttct gtcatagtct
     4801 ccacctactc tacaatcatc ttgccgtgca tcatgttcat accacaagca aagccaaact
     4861 ctccagcctg ttcaggcgtg atttccacta catactcttc ccccattggc aggttcgcat
     4921 gtacaccaaa atctggaaaa acaatttgat ccagacatgg tgaaggatcc ttgcggtcaa
     4981 agacaatgcg tgctggcact gatttcttga ggacaatcaa ctcaggagta tagcctccca
     5041 tgacttccac tcgaatctct tggtatccgt ttttttgctg ggctttttgt tcagattttt
     5101 caggcttttt gaaaaaccaa aacaagataa acgcgataag ggcaatacaa ataatggtta
     5161 caatactatt taacatgacg tctcctttac atacaattac atcttacttc tgttacagca
     5221 cttgatttct tctctgaaat cacagcttcc aagtcttcca agtcagtctg agtaaattca
     5281 cattctacaa tcaagtcagc caacaaattc ctaatcctac gggaacaaac cttgtctttg
     5341 atatcttgga caagtaaatc ccgactttgg tctagagtta aaagggctga ataaacaaag
     5401 gacttgcctt cttttttccg agtcagacac tctttctcaa ccaaacgagc caaaagtgtc
     5461 tgaacagtgg acttggacca atcaaaccgc tctgccaaaa ccctaatcaa atctgtactg
     5521 gtctgctccc cctgcatcca aataatcttc atgacctgcc attctgcatc tgaaatctgc
     5581 attaccatac ctccaaaatc tacatttgtc aattacactc atcagtatac tatcaaaatc
     5641 tacatttgtc aattatagaa ataatatttt cttcgaaaaa tagaatttta atcatttgaa
     5701 aaacgatttg cagtcaaata ttactatata aacaataaaa atatgctata ctaaagaaaa
     5761 aagaaaacaa ccactagggg tgcgtaaagc tgagattaac gactgttaga tccctctgac
     5821 tcaatctagg taatgctagc tgatggaagt ggaaatgata atggggacta gcagtcttct
     5881 attgcctttc taaaacagac tagcttgttc ttaagaatac aaacttcagt tggttgggag
     5941 gttttagatg acttatttac ccgttgcttt gaccattgca gggactgacc ctagtggtgg
     6001 tgctggcatt atggcagatt taaagtcatt ccaagcgaga gatgtctatg gaatggctgt
     6061 tgtaaccagt cttgtcgctc aaaataccag aggtgttcag ctaatcgaac acgtttctcc
     6121 tcaaatgttg aaagcccaat tggacagtgt cttttctgat attccacctc aggctgtaaa
     6181 aactggaatg ttggctacta ctgaaatcat ggaaatcatc caaccctatc ttaaaaaact
     6241 ggattgtccc tatgtccttg atcctgttat ggttgctaca agtggagatg ccttgattga
     6301 ctcaaatgct agagactatc tcaaaacaaa cttactacct ctagcaacta ttattacgcc
     6361 aaatcttcct gaagcagaag agattgttgg tttttcaatc catgaccccg aagacatgca
     6421 gcgtgctggt cgcctgattt taaaagaatt tggtcctcag tctgtggtta tcaaaggcgg
     6481 acatctcaaa ggtggtgcta aagatttcct ctttaccaag aatgaacaat ttgtctggga
     6541 aagcccacga attcaaacct gtcacaccca tggtactgga tgtacctttg ctgcagtgat
     6601 tactgctgaa ctagccaagg gcaagagtct ttaccaggca gttgataagg ccaaggcctt
     6661 tatcacaaaa gctattcaag atgcccctca actcggtcat ggttctggtc cagtcaacca
     6721 tacaactttt aaagattaag aaaaaaaact ctctagttcc cactttaagg gaattagaga
     6781 gtttttatac tcttcgaaaa tctcttcaaa ctacgtcagc ttccatctgc aacctcaaaa
     6841 cactgttttg agctgacttc gtcagtctta tctaaaacct caaggcagta ctttgagcaa
     6901 cctgcgacta gctttctagt ttactctttg attttcattg agtattaatt aggaaagaat
     6961 gtcatgcaac tttgttaaaa aggcttgcgt ttttgcctca atatcttctg cttgcatcaa
     7021 atcacgtaca acagctacac cagctatgcc agtgcccata agctgatcaa tattctccga
     7081 agtcaagcct ccaatagcaa ctactggaat ggcaaccgtt tggcaaattg ttttcaaggt
     7141 cgatatcaga gtaatgggcg cattttcctt ggtggtggtt gggaaaatgg ctcctgtacc
     7201 caagtaatct gcacctgatt tctccgcttc cagagctctt ttaaccgttt tagcggtgac
     7261 accgaggatt ttttcaggac ccaagacttg tcgggcaacc gaaactggga gttcgtcatc
     7321 tccaatatgc agacctgctg catcaaccgc aagacaaaca tccaaccgat catcgattat
     7381 caaggggacc tgataggcat ctgttatttc cttgacttgt tttgccagtt gataatattg
     7441 attggttgtg agattttttt ctcgcaattg gactatggta acccctgaac ggcaggccgt
     7501 ctcaattttt gcaagaaagc tttccacgga atcttgatag cgattggtta ccagatatag
     7561 tctaagtgct tctctattca taaacctctc ctttgatggt atctagccaa ttttcatctc
     7621 ttcttaggag cgaaagctga ttgagtactt ggtaacgaaa ttcttccaat cccattcctt
     7681 gaacaactat tttctcagca gcgatattga gataagagac tgctaagcaa gaagcttcaa
     7741 aaccagtctt tccttggctg agaaaaacag ctgttaaggc tccaaccaag tctcctgtcc
     7801 ctgttatcca gtctaattca gtacagccat ttcccagtac agcgacctga tttttcgaaa
     7861 cgacgaggtc cttgggacct gtgactaaga aagacatacc aggataggtc tgacaccagt
     7921 ctttcaagac ttgaagcaaa tcctccgttt cttgatcttt agcactcgca tcgaccccaa
     7981 cgccgtggtg ctttaatcca acaagacttc gaatttctga catgtttcct ttaaggaccg
     8041 taggtctata gtctaaaagg tctctgacta agctcttacg aatggatgaa gctgttacgc
     8101 caaccgcatc tactaccatt gggagacaaa cttgagctgc ataagaagct gctatacgga
     8161 ttgctttctc cttctcagct gacaaatgcc ccaaattgat gaagagagcc tgactttgct
     8221 tagtaaaatc aagaacctca cggggatcat ctgccatgac aggtttgcat cccagagcca
     8281 aaatcccatt tgccagcatc tcacaagaaa tctcattggt catacagtga atgagggaac
     8341 tagagcctat aggaaaagga tttgtcaatg cctgcatcat tctatccttt cagcaaagaa
     8401 atatccttgc acttttttaa agaattcctg cttgattaaa aatctaaatg caataaagga
     8461 aatcgctgta ccaatcaagg ttgctccgaa aaatcgaggc gtgtagataa accagctaag
     8521 cttagcagct gatcctgtaa agagtaccat aacaggatag gaaacaatag aaccaataat
     8581 acctgttccc acaatttctc ccaaggcaga aaagtaaaat tttcgaccgt acttataaaa
     8641 gagacctgct agaagggctc caaaagtcgc tcctgtgaga gctaaaggcg gaatcccttg
     8701 agtcgtcata cggataaagg ctgtcactgt agccatagcc aaggcataaa caggtcccat
     8761 catgattccc gctagaatat tgactacact ggacatcggt gccattccct caatccgaaa
     8821 gataggtgta aggactacat caagggcaat catcatagat aaaatggtca atttgtgaac
     8881 ttgtagttgg tgctttctca tgtttctatt cttctccttt ttctaaagac tgtaaatcgc
     8941 tcttccatgt ctggtgttgg taagccatct cccaaaactt ggcttccata tgaacactga
     9001 tgtggaaggc atctagcatt ttttgcttat ctgtctcatc actttctcga tagagctgat
     9061 tgaccagtgc tccctcctct ctgatctgtt gctctaactc atccgtaata taagtttcaa
     9121 tccattgttg atagagagga tttggtgatg gtttaagatt aagtgatttg cctatatcat
     9181 ggtataacca aggacaagga agcaagcttg caaaagctat ggctaagttc ggttctgcaa
     9241 attgcctata aatatgagaa atgtaatgat aacaggttgg agcgattgga tgttgctcca
     9301 tttcctggtc gttgatttcc aattccttga aaaattgttg gcgaataaat aactcaccct
     9361 ccactaaacc ctgagcattt tgtttcaaga gtcttttcat ctcttggttt gaagtcttat
     9421 cagccaacag atgatagatt tctgagaaag ccttcagata gtaggcatcc tgaatcaggt
     9481 aatagcggaa aatggcaggt tctaaattcc cctcttgtaa ttgtaaaata aagggatgat
     9541 gaaaggaagc ctgccaagct ttcttggata attccatcgc aatatctgta aattccataa
     9601 taactccttt ataaaaatag actggtttga agcaataaaa agaaaagcag gtagattaat
     9661 tttgtttttt taggaatata aaaagtccga tagctattct tcaactgtgc atgttcgtca
     9721 tatccgtgag cagatagagc tctcaggtaa agatggcgcc acctaaagac tgtcatcaga
     9781 accttactgt aaatcaaggg cgaccaaaaa tgtagttctt gaccacgtaa taggcaagct
     9841 tctttgaggg acttgatttc ttgctgaatg agaggaaagg aattgaatac cacaatcaag
     9901 gcataggacc aagagcgtga tagccccttt tgagccaagt acaagagaag ctcttttagt
     9961 gaaatagagg aaacaaagac aaggccgata caaactgtca caaaagccct cgttccaagc
    10021 atgactgcct gtgaagcatc tccgtgtaac tgaactgccc agtagttggc aaaagatggt
    10081 aaaatggcaa gtatgatcat ccaagctaac attttaaatc gacggtaata gagcataaag
    10141 agaatacaaa atgcgactac cgaaagattc agagcaatcg aaggaatgaa agatgtttcc
    10201 aaggataaaa tcagcaagaa gagactgata atcggtgtct gggttgctac tttgaccata
    10261 ctatctcacc tccccttggg tattgctact ctgagatgta agtggtttgg taatggtcac
    10321 ttctttcaca tgccgaagac cctgactagt catctcaatc caataatcaa ccacagaaat
    10381 caaagggtct aaacgatgac taatgagcag aaaacttctt ccttggttcc tctcctccac
    10441 aatccacttg caaaaataat ggcaggctct atcatccaaa cctgcaaaag gttcatctag
    10501 caagatcacg gaagccttac tggtcaagat ggtcaggagc tgaagaattt tttgctgacc
    10561 accacttaat tgatagggac tcttatcgac tgcctgctcc aaatcaaaat atcgtaaagc
    10621 ttgaaaaatc cgctgatttc tttcagaatc aggtccatct aattgaagct cctctcgcag
    10681 actgactcgg ataaactgct tctcagcttc ctgaacaaca cccgtcagat cacggtacaa
    10741 actctttttc tttttcagga ctgtcccctt ccatgcaatg cgccccttat acttttgaaa
    10801 ttgaagaata gaccgaaaga gggttgattt cccgacacca ttgtcaccca ggatacagga
    10861 aatcccttga tagaatgtga aatcagcaat tgaaaagagg gggcgattac caagctcacc
    10921 agtcacacgg ttcatatgga ataattccgg gctagcagca acttcctttg aagcaacctg
    10981 tgtcatctca taggaaggga tttgaaacac ttcccttagt tttccgtctc ttagctcaac
    11041 catatagtcg atataggctt tatagtcaga taaatcatgg tcgcacaaaa tgactgtctt
    11101 cccatcaaga gccaactctt ttagaatctc caatatctcg attctactct tgcggtcaat
    11161 ggaagcgaag ggctcatcca agagatagac cctaggattc atggcaaaga ggacagccag
    11221 cgctgctttt tgcttttccc cacctgatag gtgatgaatg ggacggtgta agattgcctc
    11281 gcaacgacat tgctggacaa cctctgctat tttagaatca atttcctgaa ggtgatagcc
    11341 gatattctcc aaggtgaaaa tcagctcctc aaacaagttc tccatggtaa attgatgatt
    11401 gggattttgc aagagaatgc caaccttctg gacacgtgcg atgatagaaa gctgactgac
    11461 ctcgctccca tctatcagga cttgaccact atagggaaga gaactgactt gggcaatcat
    11521 ttgaaagagg ctggattttc ctgaaccatt gctcccaact aacaaggtaa aggcttgcgc
    11581 atgaaaagta aaatcaaacg gctcagagaa gattggggac tgaatcgctc gtagttccag
    11641 acccatctat gcttttcctc cagttgcaaa ctgatgatag agtttgacaa tggcacgaac
    11701 caagatggta cagaagaaat aaacagaaat aaaacgtacc acaagcaagg aaaggacaaa
    11761 cggaagggaa aaggcgtagt aacctaactt aatgtattca tagacaaagc taacaagcgt
    11821 aatcccaata ctattagcag ttagagagag ccaactttca tagcgattct tagttacgat
    11881 aaaaccaaat tcacttccca aaccttgaac caagccagac aaaagagctc ctagaccaaa
    11941 ttggctacca taaaggactt cagcaagcgc agctagcact tctccaatcg ttgcacttcc
    12001 gactctcgga acaaagatgg cagcaatggg cgcagccata caccagagac cgaagaggat
    12061 ttcattggca aaggcctgca aaccaagagg tgttaagagt agactgagaa tattatacac
    12121 atatcctgaa ccaacgaaaa ccccaccaaa aaagatagac aagaaagcaa gcaagataac
    12181 atcttttaac tgccattttt tcaacataaa aaactccttt ttttaaagaa aagtggggca
    12241 ctcaagaaga cagacctaaa tactttgtat agcagactga atttagaaca gtacacaaga
    12301 acactaaaat atttctagaa attaatttga attttataat tgatttgttc gcatcttatt
    12361 tcaatctact atatcatctt catccagttt cgtaaaagaa aaaactctaa ttacagatac
    12421 gaattagagt tcagcttaca agattagaca gttcttttcg acatacgaaa aaaaca
//
LOCUS       DAVGJL010000054        12358 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.054, whole genome shotgun sequence.
ACCESSION   DAVGJL010000054 DAVGJL010000000
VERSION     DAVGJL010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12358
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.054"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(<1..376)
                     /locus_tag="VVE42_001215"
     CDS             complement(<1..376)
                     /locus_tag="VVE42_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEU3073413.1"
                     /translation="MQYSCGKININIPDGYGDIKDIVFSTHIIVRYNNGHCGGIDPHI
                     IGLCKKQIRRMSLYPILIIVSRDSKVIDDYKNLDIAYVDCTQCSNNFETALHVKNILK
                     LLKIQLIHCHGYSTNYFLYMLKK"
     gene            619..912
                     /locus_tag="VVE42_001216"
                     /pseudo
     CDS             619..912
                     /locus_tag="VVE42_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            1019..1370
                     /locus_tag="VVE42_001217"
                     /pseudo
     CDS             1019..1370
                     /locus_tag="VVE42_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1646..2952
                     /locus_tag="VVE42_001218"
                     /pseudo
     CDS             1646..2952
                     /locus_tag="VVE42_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011680908.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2992..4002)
                     /gene="tsaD"
                     /locus_tag="VVE42_001219"
     CDS             complement(2992..4002)
                     /gene="tsaD"
                     /locus_tag="VVE42_001219"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEU3073414.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKAFA
                     WAHGLPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLYHNAEQKGESLSTEDLCASFQAAVMDILMAKTKKALEKYPVKTLVVAGGVAANK
                     GLRERLAAEITDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFANLDLNAKPSLAFDT
                     ME"
     gene            complement(3992..4429)
                     /gene="rimI"
                     /locus_tag="VVE42_001220"
     CDS             complement(3992..4429)
                     /gene="rimI"
                     /locus_tag="VVE42_001220"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEU3073415.1"
                     /translation="MIEIKRIQQQPDLAQAIYAVMAAVYLVSPWTLEQIQADLSQDQT
                     WYALAYDGAEVIGFLAVQETLFEAEVLQIAVKGAYQGQGIASALFAQLPTDKEIFLEV
                     RQSNQRAQAFYKKEKMAVIAERKAYYHDPVEDAIIMKREIDEG"
     gene            complement(4426..5109)
                     /gene="tsaB"
                     /locus_tag="VVE42_001221"
     CDS             complement(4426..5109)
                     /gene="tsaB"
                     /locus_tag="VVE42_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEU3073416.1"
                     /translation="MKVLAFDTSSKALSLAILEDKQVLAETTINIKKNHSITLMPAID
                     FLMASLDWTPKDLDRIVVAEGPGSYTGLRIAVATAKTLAHTLNIELVGMSSLLALVPY
                     QQEGLFIPLMDARRNNVYAGFYENAKPVMPEAHLSFEEVLEKVKGASQVTFVGEVGPF
                     VEQIQEHLPRTNYKETLPNAANLALLAWDKEADSLHDFVPNYLKRVEAEENWLKNHTE
                     SGESYIKRL"
     gene            5287..5472
                     /gene="cibA"
                     /locus_tag="VVE42_001222"
     CDS             5287..5472
                     /gene="cibA"
                     /locus_tag="VVE42_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
                     /protein_id="HEU3073417.1"
                     /translation="MTNFDILDNQFLSLSENELSDIDGGLAPLVIFGVAVSWKAIAGG
                     TALIGSGLAAGYFLGGD"
     gene            5474..5626
                     /gene="cibB"
                     /locus_tag="VVE42_001223"
     CDS             5474..5626
                     /gene="cibB"
                     /locus_tag="VVE42_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HEU3073418.1"
                     /translation="MMKNLNNYREISNKELQEIKGGFGVGVGIALFMAGYAIGKDLRK
                     KFGKSC"
     gene            6444..6677
                     /locus_tag="VVE42_001224"
     CDS             6444..6677
                     /locus_tag="VVE42_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEU3073419.1"
                     /translation="MIYKVFYQETKERSPRRETTRALYLDIDASSELEGRITARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFEITEF"
     gene            6679..8358
                     /locus_tag="VVE42_001225"
     CDS             6679..8358
                     /locus_tag="VVE42_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEU3073420.1"
                     /translation="MTYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAP
                     KGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLCLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKGNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
     gene            complement(8553..10466)
                     /gene="mnmG"
                     /locus_tag="VVE42_001226"
     CDS             complement(8553..10466)
                     /gene="mnmG"
                     /locus_tag="VVE42_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEU3073421.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLSLRQTMIDEILVEDGKVVGVRTATYQEYAAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDEAPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQGKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEMGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNSKVEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEDLDDKIIELIETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
     gene            complement(10476..10931)
                     /locus_tag="VVE42_001227"
     CDS             complement(10476..10931)
                     /locus_tag="VVE42_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU3073422.1"
                     /translation="MTQQDFRTKVDNTVFGVQATALIVQNHKLLVTKDKGKYYTIGGA
                     IQVNEKTEDAVVREVKEELGVKAQAGQLAFVVENRFEQDGVSYHNIEFHYLVDLLEDA
                     PLTMQEDEKRQPCEWIDLDKFEDIQLVPVFLKTALPDWEGQLRHIHREE"
     gene            complement(11072..12193)
                     /gene="mnmA"
                     /locus_tag="VVE42_001228"
     CDS             complement(11072..12193)
                     /gene="mnmA"
                     /locus_tag="VVE42_001228"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEU3073423.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYARVVRDEDGTVHMLRGVDHGKDQTYFLSQLS
                     QEQLQKTMFPLGHLEKPEVRKLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKALSKNILYVGQ
                     GFYHDSLMSTSLEASQVHFTCDMPEEFTLECTAKFRYRQPDSKVTVHVKGDKAEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
BASE COUNT         3541 a         2867 c         2450 g         3500 t
ORIGIN      
        1 gtttttttaa catatataaa aaataattag ttgagtagcc atgacagtgt ataagttgga
       61 tttttaataa ttttaaaata tttttaacgt gtaaggcagt ttcaaaatta tttgaacatt
      121 gagtacaatc aacataggca atatctaaat ttttataatc atcaataacc tttgaatctc
      181 tagatacgat tatcaaaata ggatatagag acatccttct gatctgcttt ttacaaagtc
      241 caattatatg cggatctata cctccacaat gtccattatt atacctaact ataatatgag
      301 tcgaaaacac tatatcctta atgtctccat atccatcagg gatattaata tttatttttc
      361 cacaactata ttgcattgta accatctcct taaacgacgt attatgatat ttgatagaga
      421 aatttttatg aataactcaa taattttata gtaaatcatg cttatatctc aaagatacct
      481 attttctctt gtctcgacct tctccaaaga attgctataa tactattaca aatacatctg
      541 cactacactt caaattttag cactgtataa aaacgtttca atacactaac ttcaagaaaa
      601 cttccactat taattgaaaa agttgataga gataaattaa agatctatat tgaaactcat
      661 cccgatgctt atttgactga aatagctgct gaattcaact gtcctccaac aactattcat
      721 tacgctctaa aggctatggg atatagtcta aaaaagagcc gtacctactg cgaacaagac
      781 ccagaaaaag taaatcggtt ccttaaagaa ttgaatcact taagctacct gactcctatt
      841 tatatttatg agacaggggt tgagacctat ttttatctcg aatatgatcg agccttgagc
      901 aggcagttag tctctctgga agaagatata attatttgaa ttaagatcga gacaacgcac
      961 accagagatt gcgatactgt tatagaagta ctaatgccct tttttgtttc aatatactat
     1021 ggctccgatg acctataaag atacgatgac gagtgacttt ttcgaagctt gcttccaaaa
     1081 attcttacta cctactttag atacaccatc ccttatcatt atggacaatg caaggtttca
     1141 cagaatgaac atgtgtaagg agcagggcat agactgttac cacttcctac ctattcaccc
     1201 gagtataatc ccattgagaa aatatgggct tacatcaaaa acatctcaga ataatattgt
     1261 caaattacga tgcttttctt gaggcacttt tgtcctattc ttgtttcagc cgactatact
     1321 ccgttattgg gcagctacgg aacagtcgat gggacgatgg ggggacataa aaaaatcctc
     1381 cagttttgtt ttttataaca gtatactgga gaattgacaa tctcggtaga tacctcgtta
     1441 tagcgcggtt acttattagg cagttacaaa acaactgtga acagaaaaca ttccagagtc
     1501 agacaagact ttggaatgct ttggctctat aatttctgta gtgggtaatc ccaccccagg
     1561 aattataggg tcgtttcttg tagaaaaaaa gccccatatg acctataatg aaaagcgtct
     1621 aaccaactca ttagaaaggg ttcatatgga acaacttaag aataccacag atttgctcgg
     1681 attggaagac aaaaatatca aaatcttgtc tgttctgaaa taccaaaccc atctagtcgt
     1741 tcaggcaaag ttggattccc ccgctcctcc ttgtcctcat tgtcaaggga agatgatcaa
     1801 atacgacttc cagaaagcct ctaaaattcc gcttctcgac tgtcagggtt tacccacggt
     1861 actgcatctc aaaaagcgcc gctttcagtg caagaattgc cttaaggtgg tcgtttctca
     1921 aacatccatt gtcaagaaaa attgccagat ttccaacatg gtgagacaaa aaatcgctca
     1981 gctcctcctt gaaaagcagt ctatgactga gattgcccac agattggcgg gctcaacttc
     2041 caccgtcatc cgaaaactga gggaatttaa gtttgaaacc gattggacca agttgccaaa
     2101 agttatgagt tgggatgagt atagcttcaa aaagagcaaa atgagcttca ttgcccaaga
     2161 ttttgagtcc aaatccatcc tcgcaatttt agacgggcga actcatgcgg tgattcgaaa
     2221 ccatttccaa cgctatcaga gagaggttcg ggagctggtc gaggtcatca ccatggacat
     2281 gtacagccct tattatcggc tcgctaagca actctttcca aaggcgaaga ttgttcttga
     2341 ccgcttccac attgtccaac atctgagccg agctatgaac cgagtacgaa tccaaatcat
     2401 gaaccaattt gaccgaaaat ccttggagta tcgggcgctc aagcgctttt ggaaccctcg
     2461 ctttttcgtt tctaggctcg ggctaaatca gtccactgga ctgatttact acaccaggat
     2521 agcttcaagc tctgtcagaa acgattctat cagtccacgt ttcgaatgca cttaacccat
     2581 cgggaagtac gagataagct gctttcttac tctgagggat tacaggttca ctacgaactc
     2641 tatcaactcc tgctttttca ttttcaagag aagaatgccg accatttctt tggattgatt
     2701 gagcaagaac tgccaacggt tcatccgctt tttcaaacgg tcttttggac ttttttaagg
     2761 gatagagata agattatcaa cgcacttaag ctgccttatt ccaacgctaa acttgaagcg
     2821 accaataatt tgattaaggt tatcaagcgc aaagcctttg gtttccggaa ctttaacaat
     2881 tttaaaaaac ggattttgat gactttgaac atcaaaaaag agagtacgaa tttcgtactc
     2941 tccagattgt agcttttcgc ctacccacta cacttgacaa agagccactc tttattccat
     3001 ggtatcaaag gcaagacttg gtttggcatt gaggtccaag tttgcaaagt tttctttgtt
     3061 ccactcgctg acgctggcat aggcaatcat acctgcattg tctccgcaga gtcgcagagg
     3121 ggggatgata accttgacat ctgtgatttc ggctgctagg cgttctctga gacctttatt
     3181 ggctgccaca ccacctgcca caaccagggt tttaacagga tatttctcca aagccttctt
     3241 ggtttttgcc atgagaatgt ccataactgc tgcttggaag gaagcacaca aatcttctgt
     3301 agacaggctt tctccctttt gctcggcatt gtgataaaga ttgataaagg cagatttcaa
     3361 acctgagaag gagaactcca gattatcttc cttaatcatg gcacggggga aatcataaat
     3421 atcctgcccc tgatgagcca gctcgtcaat ctcacgacct gcaggatagg tcaagcccat
     3481 gacacgaccg accttgtcat aagcctcacc aaccgcatca tcacgggttt ccccaacaat
     3541 cttataatct cctgcctccg aaacataaac caactctgtg tgtccgccgc tgaccaagag
     3601 ggctagcaag ggaaactcca aaggctccac actctgagct gccatgaggt gcccagccat
     3661 gtgattaaca ggaatcagtg gaagtccgtg agcccaagca aaggccttgg cagctgacaa
     3721 accaactagc aaggctccga ccaagcctgg tccgtaggta accgcaacag ctgtcacgtc
     3781 ctcttcggta atccctgctt ctgccaatgc ctcctcgata caggctgtaa tgacctcgac
     3841 atggtgacga ctggctactt cgggcactac gccaccaaaa cgtttgtgac tctcaatttg
     3901 actagcaatg acattggaca agagctcatc gtcgtttttc aagacggcga cactggtctc
     3961 atcacaggat gtctcaaatg ctaaaatata tctatccttc atctatttct ctcttcatga
     4021 taatggcgtc ctcgactggg tcatggtagt aggcctttcg ctcagcgata actgccatct
     4081 tttctttctt gtaaaatgct tgcgctcgtt gatttgactg tctgacttcg aggaaaattt
     4141 ccttgtctgt cggcaattga gcaaacaagg ctgacgcaat tccctgaccc tgataagctc
     4201 ctttgacagc gatttgcagg acttctgctt caaagagagt ctcctgaaca gctagaaagc
     4261 caatcacttc tgccccatca taagccagag cataccaagt ctggtcttgg gacagatctg
     4321 cttggatttg ctccagagtc caaggactga ctaggtaaac agctgccata acagcgtaga
     4381 tggcttgagc taggtcaggc tgttgttgaa ttcgcttgat ttctatcata ggcgtttaat
     4441 gtaagactcg ccagactcgg tatggttctt gagccagttt tcctcagcct cgactcgttt
     4501 gaggtaattc ggcacaaaat catgcaagga gtctgcttcc ttgtcccaag ctaaaagagc
     4561 aagattggct gcattaggca aggtttcttt ataattagtc cttggcaagt gttcttgaat
     4621 ctgctccaca aaggggccaa cttctccgac aaaggttacc tgactagcac ccttgacttt
     4681 ttctagcacc tcttcaaaag ataggtgcgc ttctggcatg acaggtttgg cattttcata
     4741 aaatcctgca taaacattat tgcgacgcgc atccatcaag gggataaaca aaccttcttg
     4801 ttggtagggc accagagcca agagactcga cataccaacc aactcgatgt tcagggtgtg
     4861 agctaaggtc ttagcagttg ctaccgcaat tcgcaagcct gtatagctac ccggcccttc
     4921 agctaccacg attcggtcca agtccttggg tgtccaatcc aaacttgcca tcaaaaaatc
     4981 tatggcaggc ataagggtaa tactgtgatt tttcttaata ttaatcgtcg tctcggcaag
     5041 aacctgctta tcctctaaaa tagcgagaga aagagccttg ctggacgtat caaaagctaa
     5101 tactttcata acacattcct atctttttgt ctgcttacta ttatactaca aaagctggca
     5161 catgggaatt ttctttgccc ccagacaaga gtgccctcac ttaactaaaa ataatttaaa
     5221 aaaatgctca cttttccttt tcttttccga atataaaagt gaacaagaaa aaaggaggaa
     5281 agttcaatga caaattttga cattcttgac aatcaatttt tatccttatc tgaaaatgaa
     5341 ttgtcagata ttgatggcgg tctcgctccc ttggttatct ttggagtagc agtatcttgg
     5401 aaggctattg caggtggaac agcacttata ggttctggtt tggcagctgg ttatttttta
     5461 ggaggagatt aatatgatga aaaatttgaa caactatcgt gaaatttcta ataaggaatt
     5521 gcaagaaatc aagggtggct ttggtgtagg tgttggtatc gctttattta tggcaggtta
     5581 tgccattgga aaagaccttc gtaaaaagtt tggtaaatca tgctagataa gaaacacatt
     5641 tttagaagga taaattttat tgtcttcatc tcttacagtt tgctcagcat tctcaatgat
     5701 ttgaacatta ctactatccc tttaccattc gatttatctg tttgtattgt tttattttta
     5761 tgcttcaact ctatttttga gcagaacaat gactcccata aaaataataa gctttgaaaa
     5821 ttccattgtc atgtcatgtt agaaaaatgc aaagaccacc tcatcttgat agatggggtg
     5881 gaattttcgt gtcgtaaatc tactatctct acattcccaa acaaaaaacc ccagcataag
     5941 cagggcatct aagcatttaa tccaaagtaa aatacaaacc aaacgacata ggtcacgagg
     6001 aggagaaaaa gcgagtagag agtcacaaag gtaattttcc acaagaactt ggtttgtcat
     6061 cgttccagtt tggcaaatag aagatttcct ccataaacgc aagcaacaaa aacgataaaa
     6121 gctagcaaac gaactccgat agcaaaagca aataagttat acatagggca acctccctga
     6181 cttaaaatct atatggaatt atgacaagca ataaatttca cttccgttat caacataata
     6241 cattttcctt attttcgcaa acgcttacca aagaaatcgt cccctaactt tctcgtttcc
     6301 gtcttttact aatttttcat tttgtggtat aattgaaata attgtagcga atcaaggtca
     6361 atctagacac aaaatggaat gaaatcaagc aaatatctgc taaaagtttg gaataagctg
     6421 acctgtaaat agaaaggaac tatatgattt acaaagtttt ttatcaagaa acaaaagaac
     6481 gtagcccacg ccgtgaaaca acacgcgcgc tttacctaga catcgatgcc agctcagaac
     6541 ttgagggccg tatcactgct cgccaacttg tcgaagaaaa tcgcccagag tacaatatcg
     6601 aatatatcga actcttgtct gacaaattgc tcgattacga aaaagaaact ggcgccttcg
     6661 aaattacgga gttctaatat gacctacact cttaaacctg aagaagtcgg cgtttttgcc
     6721 atcggtggtc taggagaaat cgggaaaaac acttacggaa ttgaatacca agacgagatt
     6781 atcatcgtcg atgctgggat taaattccca gaagatgact tgcttggtat cgactatgtc
     6841 attcctgact actcttacat cgtggacaat atcgaccgcg tcaaggctgt tttaatcaca
     6901 cacggacacg aggaccacat tggtgggatt ccgttcctac tcaagcaagc aaatgtccct
     6961 atttatgctg gaccgcttgc cttggctttg atccgtggga aactcgaaga acacggcctc
     7021 ttgcgcaacg ccaaacttta cgaaatcaac cacaacaccg agttgacctt taaaaatctc
     7081 aaggcaactt tctttagaac gactcactct attccagagc ctttggggat tgtcattcat
     7141 actcctcaag ggaaaatcgt ctgtacgggt gactttaagt tcgactttac tccagttgga
     7201 gaacctgcgg acttgcatcg tatggctgcg cttggtgaag aaggcgtgct ctgtctcctg
     7261 tctgactcga caaatgcgga agtaccaacc tttaccaact ctgaaaaagt cgttggtcaa
     7321 tccattatga agattatcca aggtattgaa ggacgtatca tctttgcatc ctttgcctca
     7381 aatatcttcc gtctccagca ggcaacagaa gctgctgtta agactggacg caagattgcg
     7441 gtctttggtc gttctatgga aaaggccatt gtcaacggaa tcgatcttgg ctacatcaaa
     7501 gctcctaagg gaacctttat cgagccaaat gaaatcaaag attatcctgc aggagaagtt
     7561 cttatcctct gtacaggtag tcagggtgag cctatggcag ccctctctcg tatcgcaaac
     7621 ggaacccacc gtcaagtaca attacaacca ggtgataccg ttatcttctc ttctagtccc
     7681 atccctggaa acactactag cgtcaacaag ctgattaaca tcatttctga agctggtgtc
     7741 gaagttatcc acggtaaagt gaacaatatc catacatctg gacacggtgg tcagcaagag
     7801 caaaaactca tgctctgctt gattaagcca aaatacttca tgcctgtcca cggtgaatac
     7861 cgcatgcaaa aagtccacgc tggactagca gtggatactg gtgttgaaaa aggcaatatc
     7921 tttatcatga gcaatggcga tgtgcttgcc cttactgctg actcagctcg tatcgcaggt
     7981 catttcaacg cccaagatat ctatgtcgat ggaaatcgta tcggtgaaat tggcgcagct
     8041 gtcctcaaag atcgtcgcga tctatctgaa gacggtgtcg ttctggcagt cgcaactgtt
     8101 gacttcaaat cgcagatgat tctatctggt ccagacatcc tcagccgagg ctttgtctac
     8161 atgagagagt ctggcgactt gattcgccaa agccagcgta tcctcttcaa tgccattcgt
     8221 atcgcactga aaaataagga tgctagcgtg caatctgtca atggtgccat tgtcaacgct
     8281 attcgcccct tcctctatga aaataccgaa cgtgaaccga tcatcatccc gatgatcctc
     8341 acaccagatg aagaataaag caagaaaaca gccccgtcct cggagctgtt tttctctatg
     8401 ctttcttttg agattaaaac tcaatgaaaa tcagactaga aggctaagcg aaagcataac
     8461 ttgagttagc tcccatagtt cgggaaacta tgggaggctg gagatgaatc aaagccaagc
     8521 tttgaactca ttcgtaagaa gccaacgacg tatcattttg atttttgaag agttttagaa
     8581 atactacgat ttttaccttc cagatacacc atcaaaatag aaatatctgc tgggtttact
     8641 cccgaaatac ggctggcttg gccgatggtt tctggattga tgagtttgaa cttctgacga
     8701 gcttctgtcg caatagaatc gatgtcatcc cagtcgatat tggctggaat gcgtttttct
     8761 tccatacgtt tcatcttggc aacctgatcc atggctttgg aaatatagcc ttcgtacttg
     8821 atttctgttt caatcaattc gataatcttg tcatccaagt cttctgcagc tggtccgatg
     8881 aaggccacca catcttggta agaaacttct ggacggcgaa ggaattcctt ggctgtcacc
     8941 gcatctgtca acggcttgaa gcccatctcc tcaaccttgg aattggtttc cttgactggc
     9001 ttgagtttga tactgtctag gcgtttcatc tcattatcaa attgattttt cttgatttca
     9061 aaacgagccc agcgttcatc atccacaagg ccaatctcgc gtcccatctc agtcaagcgc
     9121 atatcagcat tgtcatggcg aagaatgaga cgatattcag cacgactggt caagagacgg
     9181 taaggttcaa tggttccctt ggtcactaag tcatcaatca tcacccctat ataaccatca
     9241 ctgcgcttca aaatcaactc aggtttacct tggattttca gagccgcatt gatacccgcg
     9301 ataatccctt ggccagcagc ttcttcgtaa cctgatgttc catttgtctg accagcagtg
     9361 aagagacctg agattttctt ggtttccaaa gtcgcacgca actgatgagg caagaccata
     9421 tcatactcaa tagcataacc tgtccgcatc atctctgcat tttccaaacc tttgatggaa
     9481 tgaaccaagt cacgctggac atcctcaggc agactggttg aaagtccttg aacatagact
     9541 tcctcagtat tgcgcccttc tggctcaagg aagagttggt gacgttcctt gtccgcaaag
     9601 cgcacaatct tgtcttcaat cgacggacag taacgaggcc ccactccctt gaccacacct
     9661 gtaaacatag gcgcacggtg gaggttgttt tggataatct catgactggt accattggtg
     9721 taggtcaacc agcatggcac ttggtccttg acataatcct catcacgtga agtgtatgag
     9781 aagtgattag gtgcttcatc tcctggttgg atttctgtca cgtcgtagtt gatagaagag
     9841 gccttgacac gtggaggggt tcctgtcttg aaacgaccga tttcgaggcc cagttccttg
     9901 agattgtcag ctaggttaat agaagccaag ctgtggttag gacctgatga gtacttgagg
     9961 tctccgatga taatttcccc acggagagca gtccctgtcg tcacaataac agccttagca
    10021 gcatattctt gataggtggc tgtgcgcaca ccgacaacct tgccatcttc caccaaaatc
    10081 tcatcaatca tggtctgacg aaggctcaga ttttcttgat tttcaactgt cttgcgcatc
    10141 tccttagagt aaagttcctt atcagcctgc gcacgaaggg cacgaacggc aggacccttt
    10201 cctgtgttga gcatcttcat ctggatgtaa gtcttgtcaa tagtcttggc catctcgcca
    10261 ccgagggcat cgacttcacg tacgacaatc cccttagcag aaccaccgat agagggatta
    10321 caaggcatga aagccagcat ttcaatattg atggtcgcaa gcaagacctt gcagcccata
    10381 cggctagcag ctaaggaagc ctcaacccca gcgtgtcccg caccaattac aataatatcg
    10441 tattcttcag taaaatgata agtcatgttt ctctcctatt cctcacgatg aatgtgtctt
    10501 agttggcctt cccaatctgg tagggccgtt tttaaaaaga ctggaactag ctggatatcc
    10561 tcaaacttat ccaagtcaat ccactcacag ggctgccttt tctcatcttc ctgcatggtc
    10621 aatggggcat cctccagcaa gtccaccaga taatgaaatt cgatattgtg ataggaaaca
    10681 ccgtcctgtt caaaacgatt ttcaaccaca aaagctagct gcccagcttg agctttgaca
    10741 cccagttctt ccttcacttc acggactacc gcgtcttccg ttttttcgtt aacttgaatc
    10801 gcacctccaa tagtgtaata cttgcccttg tctttggtaa ctagaagctt gtgattttgg
    10861 acaatcaagg ctgtcgcctg aacaccaaaa accgtattgt ctacttttgt ccgaaagtct
    10921 tgttgagtca ttcttgtcct ttcccttaaa cgacacaaaa acagtcaaaa ctacaaagaa
    10981 gtgcaggaca aaaaagcctg caacatccat gagctttgac catcatttct attgctgtta
    11041 ttattgtagc aaattgagaa aatttgtcaa tctaaatgta ctgacaaact tgtccatcgc
    11101 ggtaagcatt gtcaatcaaa ccgccaccta gacactcttc gccatcgtaa aagacaactg
    11161 cctgtcctgg tgtaatggcg cgttgtggtt ccgcaaagat gacctctgcc ttgtctcctt
    11221 tgacatggac ggtcacctta gagtcaggct gacggtaacg gaatttagcc gtacattcta
    11281 gcgtaaactc ctcgggcata tcacaagtaa agtggacttg gctagcctct aggctggttg
    11341 acatgagcga atcatggtag aatccttgtc ctacatagag aatattcttg cttagagctt
    11401 ttccgacaac gaaccaaggg gcattgtcac caccgtgttg cccaccgata ccaagcccgc
    11461 cacgctgacc aatcgtatag tacataagac ctgcatgctc gcccatatcg cgaccatcca
    11521 cagtcatcat gcgaccaggc tgagctggca ggtagttgct gagaaagttt ttaaagttct
    11581 tttctccgat aaagcaaatc cctgtcgagt ctttcttctt agcagtcgaa agacctgctt
    11641 cttctgctag ttttcgaact tcaggctttt ccaaatgtcc tagtgggaac atggtttttt
    11701 gaagttgttc ttgcgaaagt tggctgagga aataggtctg atccttgcca tggtccacgc
    11761 cacgaagcat gtgaacggta ccatcctcat cacgcaccac tcgagcataa tgcccagtcg
    11821 ctacatagtc tgcccccaag gtcatagcat agtccaaaaa ggccttgaac ttgatttcct
    11881 tgttgcacat aacgtccgga tttggcgtgc gccctgcacg gtattccgct aggaaatact
    11941 caaaaacgcg gtcccagtac tctttttcaa aattgacaga gtagtaggga atgccaatct
    12001 ggtctgccac cgcagccaca tccttgtaat cttcggtcgc cgtacagacg ccgttttcat
    12061 ctgtgtcatc ccagttcttc atgaagatac cgatcacatc gtagccctgc tccttgagca
    12121 aaagagccgt caccgacgaa tcaacaccac cactcatccc cacgacaaca cgtgttttag
    12181 agttatcact catggtaagt ctcccatcta ttcgtttatt cacgattgaa ggtcgtgtgt
    12241 gcttcacgat tgaaggtcgc ttgagcagta ttcattataa cacgcttggt tagagaagac
    12301 aaggaagagc ttaaaaattg ttttttaaaa cttatagtca atatacagat tatgtgaa
//
LOCUS       DAVGJL010000055        12290 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.055, whole genome shotgun sequence.
ACCESSION   DAVGJL010000055 DAVGJL010000000
VERSION     DAVGJL010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12290
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.055"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..656
                     /locus_tag="VVE42_001229"
     CDS             <1..656
                     /locus_tag="VVE42_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU3073424.1"
                     /translation="QITDKTLNIKIVVKDFARNTTVKEFILNKDTGEVSELKPHRVTV
                     TIQNGKEMSSTIVSEEDFILPVYKGELEKGYQFDGWKISGFEGKKDAGYVINLSKDTF
                     IKPVFKKIEEKKEEENKPTFDVSKKKDNPQVNHSQLNESHRKEDLQREDHSQKSDSTK
                     DVTATVLDKNNISSKSTTNNPNKLPKTGTASGAQTLLAAGIMFIVGIFLGLKKKNQD"
     gene            1282..1758
                     /locus_tag="VVE42_001230"
     CDS             1282..1758
                     /locus_tag="VVE42_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU3073425.1"
                     /translation="MVLDYFFDKNLVFCLEADNQEQLFDQVATLLEEREIVTPTYREA
                     LITREKSFPTGLDMEFLGKDLPNVAIPHTDIVHNLAEKVVVVRLEKPVTFHNMIAPDK
                     EVEVSLLFFIINNSSSSQTNILAQLMDFFTGNGHLEDLSKISEPEKLYAYIAEATA"
     gene            1796..2101
                     /locus_tag="VVE42_001231"
     CDS             1796..2101
                     /locus_tag="VVE42_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU3073426.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPAMSAPVFDNIESAIKEHGLS"
     gene            2149..3624
                     /locus_tag="VVE42_001232"
     CDS             2149..3624
                     /locus_tag="VVE42_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEU3073427.1"
                     /translation="MDAIFDLIGKVFNPILEMGGPVIMLIILTVLALLFGVKFSKALE
                     GGIKLAIALTGIGAIIGMLNGAFSASLAKFVENTGIQLSITDVGWAPLATITWGSAWT
                     LYFLLIMLIVNVVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQGVSLFIA
                     TAAIVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFF
                     PGLDKYDFDAAKLNKKIGFWGSKFFIGFILGIVIGIMGTPHPIAGVADADKWRLVIKG
                     WLSLGLTAGVSLELFSLIGSWFIAAVEPLSQGITNVATKRLQGRKFNIGLDWPFIAGR
                     AEIWACANVLAPIMLIEAVLLSKVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFG
                     TLLLPLFLLSGTLIAPFATELAKGVGAFPEGVSQTQLITHSTLEGPIEKLLGWTIGNT
                     TTGDIKAILGAVAFLVFYIGIFAWYRKQMIKRNEEYAAKAK"
     gene            3650..3727
                     /locus_tag="VVE42_001233"
                     /pseudo
     CDS             3650..3727
                     /locus_tag="VVE42_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            3929..10630
                     /locus_tag="VVE42_001234"
     CDS             3929..10630
                     /locus_tag="VVE42_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEU3073428.1"
                     /translation="MGKGHWNRKRVYSIRKFAVGACSVMIGTCAVLLGGNIAGESVVY
                     ADETLITHTAEKPKEEKMIVEEKADKALETKNVVERTEQSEPSSTEAIASEKKEDEAV
                     TPKEEKVSAKPEEKAPRIESQASSQEKPLKEDAKAVTNEEVNQMIEDRKVDFNQNWYF
                     KLNANSKEAIKPDADVSTWKKLDLPYDWSIFNDFDHESPAQNEGGQLNGGEAWYRKTF
                     KLDEKDLKKNVRLTFDGVYMDSQVYVNGQLVGHYPNGYNQFSYDITKYLHKDGRENVI
                     AVHAVNKQPSSRWYSGSGIYRDVTLQVTDKVHVEKNGTTILTPKLEEQQHGKVETHVT
                     SKIVNTDDKDHELVAEYQIVERGGHAVTGLVRTASRTLKAHESTSLDAILEVERPKLW
                     TVLNDKPALYELITRVYRDGQLVDAKKDLFGYRYYHWTPNEGFSLNGERIKFHGVSLH
                     HDHGALGAEENYKAEYRRLKQMKEMGVNSIRTTHNPASEQTLQIAAELGLLVQEEAFD
                     TWYGGKKPYDYGRFFEKDATHPEARKGEKWSDFDLRTMVERGKNNPAIFMWSIGNEIG
                     EANGDAHSLATVKRLVKVIKDVDKTRYVTMGADKFRFGNGSGGHEKIADELDAVGFNY
                     SEDNYKALRAKHPKWLIYGSETSSATRTRGSYYRPERELKHSNGPERNYEQSDYGNDR
                     VGWGKIATASWTFDRDNAGYAGQFIWTGTDYIGEPTPWHNQNQTPVKSSYFGIVDTAG
                     IPKHDFYLYQSQWVSVKKKPMVHLLPHWNWENKELASKVADSEGKIPVRAYSNASSVE
                     LFLNGKSLGLKTFNKKQTSDGRTYQEGANANELYLEWKVAYQPGTLEAIARDESGKEI
                     ARDKITTAGKPAAVRLIKEDHAIAADGKDLTYIYYEIVDSQGNVVPTANNLVRFQLHG
                     QGQLVGVDNGEQASRERYKAQADGSWIRKAFNGKGVAIVKSTEQAGKFTLTAHSDLLK
                     SNQVTVFTGKKEGQEKTVLGTEVARVRTLIGKEPKMPKTVGFVYSDGSRAERPVTWSS
                     VDVSKPGIVTVKGMADGREVEARVEVIALKSELPVVKRIAPNTDLNSVDKSVSYVLTD
                     GSVEEYEVDKWEIAEEDKAKLAIPGSRIQATGYLEGQPIHATLVVEEGNPAAPAVPTV
                     TVGGEAVTGLTSQKPMQYRTLAYGAKLPEVTASAKNAAVTVLQASAANGMRASIFIQP
                     KDGGPLQTYAIQFLEEAPKIAHLSLQVEKADSLKEDQTVKLSVRAHYQDGTQAVLPAD
                     KVTFSTSGEGEVAIRKGMLELHKPGAVTLNAEYEGATGQVELTIQANTEKKIAQSIRP
                     VNVVTDLHQEPSLPATVTVEYDKGFPKTHKVTWQAIPKEKLDSYQTFEVLGKVEGIDL
                     EARAKVSVEGIVSVEEASVTTPIAEAPQLPESVRTYDSNGHVSSAKVAWDAIRPEQYA
                     KEGVFTVNGRLEGTQLTTKLHVRVSAQTEQGANISDQWTGSELPLAFASDSNPSDPVS
                     NVNDKLISYNNQPANRWTNWNRTNPEASVGVLFGDSGILSKRSVDNLSVGFHEDHGVG
                     APKSYVIEYYVGKTVPTAPKNPSFVGNEDHVFNDSANWKPVTNLKVPAQLKAGEMNHF
                     SFDKVETYAVRIRMVKADNKRGTSITEVQIFAKQVAASKQGQTRIQVDGKDLANFNPD
                     LTDYYLESVDGKVPAVTASVSNNGLATVVPSVREGEPVRVIAKAENGDILGEYRLHFT
                     KDKNLLSHKPVAAVKQARLLQVGQALELPTKVPVYFTGKDGYETKDLTVEWEEVPAEN
                     LTKAGQFTVRGRVLGSNLVAEVTVRVTDKLGETLSDNPNYDENSNQAFASATNDIDKN
                     SHDRVDYLNDGDYSENHRWTNWSARPSTNPEVSAGVIFRENGKIVERTVAQAKLHFFA
                     DSGTDAPSKLVLERYVGPGFEVPTYYSNYQAYESGHPFNNPENWEAVPYRADKDIAAG
                     DEINVTFKAVKAKAMRWRMERKADKSGVAMIEMTFLAPSELPQESTQSKILVDGKELA
                     DFAENRQDYQITYKGQRPKVSVEENNQVASTVVDSGEDSLPVLVRLVSESGKQVKEYR
                     IHLTKEKPVSEKTVAAVQENLPKLEFVEKDLAYKTVEKKDSTLYLGETRVEQEGKTGK
                     ERIFTAINPDGSKEEKLREVVEAPTDRIVLVGTKPVAQEAKKPQVSEKADTKPIDSSE
                     ASQTNKAQLPNTGSAASQVAVAAGLALLGLSAGLVVTKGKKED"
     gene            complement(10878..11734)
                     /locus_tag="VVE42_001235"
                     /pseudo
     CDS             complement(10878..11734)
                     /locus_tag="VVE42_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007722232.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(11945..12289)
                     /locus_tag="VVE42_001236"
     CDS             complement(11945..12289)
                     /locus_tag="VVE42_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEU3073429.1"
                     /translation="MKLASILLLILTLVVCIILTKLFRLKKLGRNFADLAFPVLVFEY
                     YLITAKTFTHNFLPRLGLALSILAIILVFFFLLKKRSFYYPKFIKFFWRTGFLLTLIM
                     YIEMIVELFLIK"
BASE COUNT         4010 a         2265 c         2602 g         3413 t
ORIGIN      
        1 aacaaataac agacaagaca ctaaacatta agattgttgt aaaagatttt gcaagaaata
       61 caaccgtaaa agaattcatt ttaaataaag atacgggaga ggtaagtgaa ttaaaacctc
      121 atagggtaac tgtgaccatt caaaatggaa aagaaatgag ttcaacgata gtgtcggaag
      181 aagattttat tttacctgtt tataagggtg aattagaaaa aggataccaa tttgatggtt
      241 ggaaaatttc tggtttcgaa ggtaaaaaag acgctggcta tgttattaat ctatcaaaag
      301 atacctttat aaaacctgta ttcaagaaaa tagaggagaa aaaggaggaa gaaaataaac
      361 ctacttttga tgtatcgaaa aagaaagata acccacaagt aaaccatagt caattaaatg
      421 aaagtcacag aaaagaggat ttacaaagag aagatcattc acaaaaatct gattcaacta
      481 aggatgttac agctacagtt cttgataaaa acaatatcag tagtaaatca actactaaca
      541 atcctaataa gttgccaaaa actggaacag caagcggagc ccagacacta ttagctgccg
      601 gaataatgtt tatagtagga atttttcttg gattgaagaa aaaaaatcaa gattaggaca
      661 aaagctatag aaaaaaatgg tttatgtact gagattagat agtgaggtga tgacatagtt
      721 ttgtgaaaac agccatttat aactcaatta tttagtttac ttcactattg atactatttg
      781 gagttattaa tggacttagt ttagataact aatgaattga ttgaaagggt tagtattgac
      841 aatattggtc atattgatta gaaaatagag tccatcaaaa tttaaaggct aatagaggtg
      901 atgagacaat ttcaaggaat taagtagcgt ttggttgctt aattcctttt tatttggaag
      961 gaagtttttc gtaaccgccc aataacgaag tctattggac ggttacgata tgatcactga
     1021 cttcgtcagt cttatctaca acctcaaaac tgtgttttga gcaacctgcg gctagcttcc
     1081 tagtttgctc tttgattttc attgagtatt agaacagaaa aatgcttgga gtatttgttt
     1141 gtgtgtttat ttttatataa caaactataa acaaaacaaa aatataaaaa aagagacaaa
     1201 aaagaacaaa aagtaattga caacggtttc atatagtgtt atacttatgg cataaagtta
     1261 atggaaagaa gggaaaacct tatggtatta gattatttct ttgacaaaaa ccttgtgttt
     1321 tgcttagaag cggataatca agaacaactc tttgatcagg ttgcaacttt attggaagaa
     1381 cgagaaattg tcactccaac ttatcgtgaa gccttgatca cgcgtgaaaa gtcatttcca
     1441 actggtttag atatggaatt tctaggaaag gacttgccaa atgtagcgat tcctcataca
     1501 gatattgttc ataatctagc tgagaaagtg gtggttgttc gattagaaaa accagtaact
     1561 tttcacaata tgatagctcc tgataaggaa gtagaagtat ccttgctctt ctttatcatc
     1621 aataactcaa gttcaagtca aacaaatatt ctggctcagt tgatggactt tttcacagga
     1681 aatggacatc ttgaagacct atcaaaaatt tccgaaccag aaaaacttta tgcttacatt
     1741 gctgaagcaa ccgcttaatc ttgtctatta aaaaataaaa tcggaggaaa tctaaatgat
     1801 taaaattctt gctgcctgcg gtgcaggtgt taactcaagt caccaaatta aaagtgctct
     1861 agaagaagaa ctttcaaacc gtggttatga tgttcactgt gatgcagtca tggtgaaaga
     1921 tgtaaacgaa gaccttatga aaggttacga tatctttaca ccaatcgctg caacagacct
     1981 tggttttgaa ccaggtatcc cagttatcga agctgggcca atcttattcc gtatcccagc
     2041 aatgagcgct ccagtatttg acaatattga atcagctatt aaagaacacg gattaagcta
     2101 attctaattt gttaacattc atataacata aaaaagggag gaactgttat ggatgcaatc
     2161 tttgacctaa tcggaaaggt tttcaatccc atcttagaaa tgggtggacc tgtcatcatg
     2221 ttaatcattt tgacagtatt ggctttactt tttggagtga aattctccaa agcgcttgaa
     2281 ggtggtatca aacttgccat cgctcttaca ggtatcggtg ctatcatcgg tatgctaaat
     2341 ggtgctttct cagcatcact tgcaaaattc gttgaaaaca ctggtatcca attgagtatt
     2401 accgacgttg gttgggcacc actcgcaaca atcacttggg gttctgcttg gacactatac
     2461 ttcttgctca tcatgttgat tgtcaacgta gtgatgcttg ctatgaagaa aaccgataca
     2521 cttgatgtcg atatctttga tatctggcac ttgtctatca caggtctctt gattaaatgg
     2581 tatgctgata acaatggtgt gagtcaaggg gtttcactct ttattgctac agcagctatc
     2641 gtccttgtcg gtgtgttgaa aattatcaac tctgacttga tgaaacctac atttgatgac
     2701 cttcttaacg ccccaagttc atcaccaatg acatcaactc acatgaacta catgatgaac
     2761 ccagttatca tggttttgga taagattttt gaaaaattct tcccaggcct tgataaatat
     2821 gactttgatg ctgctaaatt gaacaagaaa atcggtttct ggggatctaa attcttcatc
     2881 ggtttcatcc ttggtatcgt tatcggtatt atgggaactc cacatccaat tgcaggtgtt
     2941 gcagatgcag ataaatggcg tcttgttatc aaaggatggt tgtctcttgg tttgactgcc
     3001 ggtgtatctt tggaactctt ctcacttatc ggttcatggt tcatcgcagc cgtagaacca
     3061 ctatcacaag gtattacaaa cgttgctact aaacgtcttc aaggacgtaa attcaatatc
     3121 ggtcttgact ggccattcat cgctggtcgt gctgaaatct gggcttgtgc caacgtactt
     3181 gcaccaatca tgttgattga agcagtgctt ctttcaaaag ttggaaatgg tatcttgcca
     3241 cttgcaggta tcatcgctat gggtgttact ccagctctct tggttgtaac tcgtggtaaa
     3301 ttgctccgta tgattatctt cggaacactc ttgttgccac tcttccttct ttcaggtacc
     3361 cttattgcac catttgcaac agaacttgct aaaggtgtag gtgccttccc agaaggtgtg
     3421 agccaaactc aattgattac tcactctact cttgaaggac caatcgaaaa acttcttggt
     3481 tggacaattg gtaacactac aactggtgat atcaaagcaa tccttggtgc agtagccttc
     3541 cttgtattct atatcggtat ctttgcttgg tacagaaaac aaatgatcaa acgtaacgaa
     3601 gagtacgcag caaaagcaaa ataatgcgct cctataaaat ataaactcaa tgaaaatcaa
     3661 agagcaaact aggaaactag ccacaggttg ctcaaaacac agttttgagg ttgtagataa
     3721 gactgacgaa atcagctcaa aacatggttt tgaggttgta gatgaaactg acgaagtcag
     3781 taatcatatc tacggcaagg cgacgctgac gaagtttgaa ttggattttc gcagagtgta
     3841 aattgttgaa actaggttgt cataccatgt ataccacttg ggctttggta gtagctaact
     3901 gcactaaata taatataagg agggttttat ggggaaaggc cattggaatc ggaaaagagt
     3961 ttatagcatt cgtaagtttg ctgtgggagc ttgctcagta atgattggga cttgtgcagt
     4021 tctattagga ggaaatatag ctggagaatc tgtagtttat gcggatgaaa cacttattac
     4081 tcatactgct gagaaaccta aagaggaaaa aatgatagta gaagaaaagg ctgataaagc
     4141 tttggaaact aaaaatgtag ttgaaaggac agaacaaagt gaacctagtt caactgaggc
     4201 tattgcatct gagaagaaag aagatgaagc cgtaactcca aaagaggaaa aagtgtctgc
     4261 taaaccggaa gaaaaagctc caaggataga atcacaagct tcaagtcaag aaaaaccgct
     4321 caaagaagat gctaaagctg taacaaatga agaagtgaat caaatgattg aagacaggaa
     4381 agtggatttt aatcaaaatt ggtactttaa actcaatgca aattctaagg aagccattaa
     4441 acctgatgca gacgtatcta cgtggaaaaa attagattta ccgtatgact ggagtatctt
     4501 taacgatttc gatcatgaat ctcctgcaca aaatgaaggt ggacagctca acggtgggga
     4561 agcttggtat cgcaagactt tcaaactaga tgaaaaagac ctcaagaaaa atgttcgcct
     4621 tacttttgat ggcgtctaca tggattctca agtttatgtc aatggtcagt tagtggggca
     4681 ttatccaaat ggttataacc agttctcata cgatatcacc aaataccttc acaaagatgg
     4741 tcgtgagaat gtgattgctg tccatgcagt caacaaacag ccaagtagcc gttggtattc
     4801 aggaagtggt atctatcgtg atgtgacttt acaagtgaca gataaggtgc atgttgagaa
     4861 aaatgggaca actattttaa caccaaaact tgaagaacaa caacatggca aggttgaaac
     4921 tcatgtgacc agcaaaatcg tcaatacgga cgacaaagac catgaacttg tagccgaata
     4981 tcaaatcgtt gaacgaggtg gtcatgctgt aacaggctta gttcgtacag cgagtcgtac
     5041 cttaaaagca catgaatcaa caagcctaga tgcgatttta gaagttgaaa gaccaaaact
     5101 ctggaccgtt ttaaatgaca aacctgcctt gtacgaattg attacgcgtg tttaccgtga
     5161 cggtcaattg gttgatgcta agaaggattt gtttggttac cgttactatc actggactcc
     5221 aaatgaaggt ttctctttga atggtgaacg tattaaattc catggagtat ccttgcacca
     5281 cgaccatggg gcgcttggag cagaagaaaa ctataaagca gaatatcgcc gtctcaaaca
     5341 aatgaaggag atgggagtta actccatccg tacaacccac aaccctgcta gtgagcaaac
     5401 cttgcaaatc gcagcagaac taggtttact cgttcaggaa gaggcttttg atacttggta
     5461 tggtggcaag aaaccttatg actatggacg tttttttgaa aaagatgcca ctcacccaga
     5521 agctcgaaaa ggtgaaaaat ggtctgattt tgacctacgt accatggtcg aaagaggcaa
     5581 aaacaaccct gctatcttca tgtggtcaat tggtaatgaa ataggtgaag ctaatggtga
     5641 tgcccactct ttagcaactg ttaaacgttt ggtcaaggtt atcaaggatg ttgataagac
     5701 tcgctatgtt accatgggag cagataaatt ccgtttcggt aatggtagcg gagggcatga
     5761 gaaaattgct gatgaactcg atgctgttgg atttaactat tctgaagata attacaaagc
     5821 ccttagagct aagcatccaa aatggttgat ttacggttca gaaacatcat cagcaacccg
     5881 tacacgagga agttactatc gccctgaacg tgaattgaaa catagcaatg gacctgagcg
     5941 taattatgaa cagtcagatt atggaaatga tcgtgtgggt tgggggaaaa tagcaaccgc
     6001 ttcatggact tttgaccgtg acaacgctgg ctatgctgga cagtttatct ggacaggtac
     6061 ggactatatt ggtgaaccta caccatggca caaccaaaat caaactcctg ttaagagctc
     6121 ttactttggt atcgtagata cagccggcat tccaaaacat gacttctatc tctaccaaag
     6181 ccaatgggtt tctgttaaga agaaaccgat ggtacacctt cttcctcact ggaactggga
     6241 aaacaaagaa ttagcatcca aagtagctga ctcagaaggt aagattccag ttcgtgctta
     6301 ttcgaatgct tctagtgtag aattattctt gaatggaaaa tctcttggtc ttaagacttt
     6361 caataaaaaa caaaccagcg atgggcggac ttaccaagaa ggtgcaaatg ctaatgaact
     6421 ttatcttgaa tggaaagttg cctatcaacc gggtaccttg gaagcaattg ctcgtgatga
     6481 atctggcaag gaaattgctc gagataagat tacgactgct ggtaagccag cggcagttcg
     6541 tcttattaag gaagaccatg cgattgcagc agatggaaaa gacttgactt acatctacta
     6601 tgaaattgtt gacagccagg ggaatgtggt tccaactgct aataatctgg ttcgcttcca
     6661 attgcatggc caaggtcaac tggtcggtgt agataacgga gaacaagcca gccgtgaacg
     6721 ctataaggcg caagcagatg gttcttggat tcgtaaagca tttaatggta aaggtgttgc
     6781 cattgtcaaa tcaactgaac aagcagggaa attcaccctg actgcccact ctgatctctt
     6841 gaaatcgaac caagtcactg tctttactgg taaaaaagaa ggacaagaga agactgtttt
     6901 gggaacagaa gtagcaagag ttcgtacatt aattggtaaa gaaccaaaaa tgccgaaaac
     6961 agtaggtttt gtatacagcg atggtagtcg tgcagaacgt cctgtaacct ggtcttcagt
     7021 agatgtgagc aagcctggta ttgtaacggt gaaaggtatg gctgacggac gagaagtaga
     7081 agctcgtgta gaagtgattg ctcttaaatc agagctacca gttgtgaaac gtattgctcc
     7141 aaatactgac ttgaattctg tagacaaatc tgtttcctat gttttgactg atggaagtgt
     7201 tgaagagtat gaagtggaca agtgggagat tgccgaagaa gataaagcta agttagcaat
     7261 tccaggttct cgtattcaag cgaccggtta tttagaaggt caaccaattc atgcaaccct
     7321 tgtggtagaa gaaggcaatc ctgcggcacc tgcagtacca actgtaacgg ttggtggtga
     7381 agctgtcaca ggtcttacta gtcaaaaacc aatgcaatac cgcactcttg cttatggagc
     7441 taagttgcca gaagtcacag caagtgctaa aaatgcagct gttacagttc ttcaagcaag
     7501 cgcagcaaac ggcatgcgtg cgagcatctt tattcagcct aaagatggtg gccctcttca
     7561 aacctatgca attcaattcc ttgaagaagc gccaaaaatt gctcacttga gcttgcaagt
     7621 ggaaaaagct gacagtctca aagaagacca aactgtcaaa ttgtcggttc gagctcacta
     7681 tcaagatgga acgcaagctg tattaccagc tgataaagta accttctcta caagtggtga
     7741 aggggaagtc gcaattcgta aaggaatgct tgagttgcat aagccaggag cagtcactct
     7801 gaacgctgaa tatgagggag ctacaggtca agttgaactc actatccaag ccaatactga
     7861 gaagaagatt gcgcaatcca tccgtcctgt aaatgtagtc acagatttgc atcaggaacc
     7921 aagtcttcca gcaacagtaa cagttgagta tgacaaaggt ttccctaaaa ctcataaagt
     7981 cacttggcaa gctattccga aagaaaaact agactcctat caaacatttg aagtactagg
     8041 taaagttgaa ggaattgacc ttgaagcgcg tgcaaaagtc tctgtagaag gtatcgtttc
     8101 agttgaagaa gccagtgtga caactccaat cgcagaagca ccacaattac cagaaagtgt
     8161 tcggacatat gattcaaatg gtcacgtttc atcagctaaa gttgcatggg atgcgattcg
     8221 tccagagcaa tacgctaagg aaggtgtctt tacagttaat ggtcgcttag aaggtacgca
     8281 attaacaact aaacttcatg ttcgcgtatc tgctcaaact gagcaaggtg caaacatttc
     8341 tgaccaatgg accggttcag aattgccact tgcctttgct tcagactcaa atccaagcga
     8401 cccagtttca aatgttaatg acaagctcat ttcctacaat aaccaaccag ccaatcgttg
     8461 gacaaactgg aatcgtacta atccagaagc ttcagtcggt gtcctattcg gagattcagg
     8521 tattttgagc aaacgttcag ttgataactt aagcgttggc ttccatgaag accatggagt
     8581 tggtgcaccg aagtcttatg taattgagta ttatgttggt aagactgtcc caacagctcc
     8641 taaaaaccct agttttgttg gtaatgagga ccatgtcttt aatgattctg ccaactggaa
     8701 accagttact aatctaaaag tccctgctca actcaaggct ggagaaatga accactttag
     8761 ctttgataaa gttgaaacct atgctgttcg tattcgcatg gttaaagcag ataacaagcg
     8821 tggaacgtct atcacagagg tacaaatctt tgcgaaacaa gttgcggcat ccaaacaagg
     8881 acaaacaaga atccaagttg acggcaaaga cttagcaaac ttcaaccctg atctgacaga
     8941 ctactacctt gagtctgtag atggaaaagt tccggcagtc acagcaagtg ttagcaacaa
     9001 tggtctcgct accgtcgttc caagcgttcg tgaaggtgag ccagttcgtg tcatcgcgaa
     9061 agctgaaaat ggcgacatct taggagaata ccgtctgcac ttcactaagg ataagaactt
     9121 actttctcat aaaccagttg ctgcggttaa acaagctcgc ttgctacaag taggtcaagc
     9181 acttgaattg ccgactaagg ttccagttta cttcacaggt aaagacggct acgaaacaaa
     9241 agacctgaca gttgaatggg aagaagttcc agcggaaaat ctgacaaaag caggtcaatt
     9301 tactgttcga ggccgtgtcc ttggtagtaa ccttgttgct gaggtcactg tacgagtgac
     9361 agacaaactt ggtgagactc tttcagataa ccctaactat gatgaaaaca gtaaccaggc
     9421 ctttgcttca gcaaccaatg atattgacaa aaactctcat gaccgcgttg actatctcaa
     9481 tgacggagat tattcagaaa atcatcgttg gacaaactgg tctgcaagac cgtctaccaa
     9541 tccagaagta tcagcaggcg taatcttccg tgaaaatggt aagattgtcg aacggactgt
     9601 tgcgcaagcc aaacttcatt tctttgcaga tagtggtaca gatgcaccat ctaaacttgt
     9661 tttggaacgt tatgtcggcc caggctttga agtacctacc tactattcaa actaccaagc
     9721 ctacgaatct ggacatccat ttaacaatcc agaaaattgg gaagctgtgc cttatcgtgc
     9781 ggataaagac atcgcagctg gtgatgaaat caacgtaaca tttaaagctg tcaaagccaa
     9841 agccatgaga tggcgtatgg agcgtaaggc tgacaagagc ggtgttgcga tgattgagat
     9901 gaccttcctt gcaccaagtg aattgcctca agaaagcacg caatcaaaga ttcttgtaga
     9961 tggaaaagaa cttgctgatt tcgctgaaaa tcgtcaagac tatcaaatta cctataaagg
    10021 tcaacggcca aaagtctcag ttgaagaaaa caatcaagta gcttcaactg tggtagatag
    10081 tggagaagat agccttccag tacttgttcg cctcgtttca gaaagtggaa aacaagtcaa
    10141 ggaataccgt atccacttga ctaaggaaaa accagtttct gagaagacag ttgctgctgt
    10201 acaagaaaat cttccaaaac tcgaatttgt tgaaaaagat ttggcataca agacagttga
    10261 gaaaaaagat tcaacactgt atctaggtga aactcgtgta gaacaagaag gaaaaactgg
    10321 taaagaacgt atctttacag cgattaatcc tgatggaagt aaggaagaaa aactccgtga
    10381 agtggtagaa gctccgacag accgcatcgt cttggttgga accaaaccag tagctcaaga
    10441 agctaaaaaa ccacaagtgt cagaaaaagc agatacaaaa ccaattgatt caagtgaagc
    10501 tagtcaaact aataaagccc agttaccaaa tacaggtagt gcggcaagcc aagtagcagt
    10561 agcagcaggt ttagctcttc taggtttgag tgcaggatta gtagttacta aaggtaaaaa
    10621 agaagactag aaattttgat accttcttta tcattaaaga ccaaagaaaa actcttgtcc
    10681 taaatagaag ataaagagtc tgggacaaaa tagttctcaa ccacaaaaaa gctagatatt
    10741 tccaattgtg gaactctagc tttttaattt tgagtcgttc tcttattgta tagtggattg
    10801 aaactagaat agtacacttc tgcttctaaa acattgttag aaatcgattt gactgtcctt
    10861 atcgatttgt cctgttctta tttcatttca ctatatttta ggaggttatt ggccataagt
    10921 accaatccca tgtcaattct cacttgacgc ttgcccctca gattacatct cttgtgaccc
    10981 aaacaagcct ttatctgccc aaagacaggt tccacatcaa tcttgcgttg agcgaaaatc
    11041 tgtctacctt ggggagataa aagcgcctga cattctttag ctttcaagtt ttgatagcgt
    11101 tcgttcatat acagtccctt ttgaggggct gattcaggtt cgtcggcgta gtaaaccttg
    11161 atttcctgtt gaaagtctgt ctgtgttttc tgatgtttgg tatggtgaaa acgataatac
    11221 cagccatcag gatgtgtgta gctatcctcc ttgtcattat agtgccaatt ccctaagttt
    11281 ctagctgact gtttataccc tctcttctgt tccttatcaa acatggcata tttaatcaga
    11341 aggggagtga tttcttcttg aaaatagact tgtatctgtt cttgaagttt ggcggagaat
    11401 ttctctgtcg ttttcttcca cacaaaacta tacttgttgg cattggcttc aatcttagtt
    11461 ccgtcaataa acaaacaatc taaggtcact aactcttcca tttttcaatt tttcgaccag
    11521 agaaaatccc ttgtgaatag gcaaatagaa gagtagatac aagcatttta aggtgataag
    11581 acgggcgatc aaaggcatga tagaaggcgt agaagtgatg ttcctccaag gtattcacca
    11641 ctttttcaat agtaaagacg aaatggtctt gtggtaagaa ggaactgatt tctagtggta
    11701 aagttgtttg atttgtgtta tagtgaatat gcatgggata gcctttctaa atggtttatt
    11761 aagcaattct attttaccaa gactatcgta accatgcaaa aaagcaacgg caaatccgtt
    11821 gccttttttg gatataagag actattgtcc cagactcttt tctttgtata aaattgtata
    11881 gtgttattgt ctatactatt ttgtagagaa agcaaaaatc cctagaaaat gcttagggat
    11941 tcgactattt tattaagaac aattcaacaa tcatctctat atacatgata agggttaata
    12001 aaaatcctgt acgccagaag aatttgatga atttaggata gtaaaagctg cgttttttca
    12061 aaaggaagaa aaagacgaga ataatggcta ggattgagag ggctagcccc agtctaggga
    12121 ggaaattatg ggtaaaggtt ttagctgtaa tcaggtaata ttcaaatacc aagaccggaa
    12181 aagccaaatc cgcaaagttt cgtcctagtt tttttaatct aaaaagtttg gttaggataa
    12241 tgcagacgac taaggtcagt atcaataata aaatagatgc taatttcatt
//
LOCUS       DAVGJL010000056        12274 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.056, whole genome shotgun sequence.
ACCESSION   DAVGJL010000056 DAVGJL010000000
VERSION     DAVGJL010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12274
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.056"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            18..701
                     /locus_tag="VVE42_001237"
     CDS             18..701
                     /locus_tag="VVE42_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU3073430.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQIGILGVDFSRLIPGILII
                     SMNSGAYVSETVRAGINAVPKGQLEAAYSLGIRPKNAMRYVILPQAVKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KHMGQGDKK"
     gene            701..1435
                     /locus_tag="VVE42_001238"
     CDS             701..1435
                     /locus_tag="VVE42_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU3073431.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGINLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKLIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVMENI
                     TLSPIKTKGESREVAEKRAQELLEKVGLPDKADAYPQSLSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQEQRTKDFLSKVL"
     gene            1589..2446
                     /locus_tag="VVE42_001239"
     CDS             1589..2446
                     /locus_tag="VVE42_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEU3073432.1"
                     /translation="MTQIIDGKALAAKLQGQLAEKTAKLKEETALVPGLVVILVGDNP
                     ASQVYVRNKERSALAAGSRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPK
                     HIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIDLEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLAHSRTHNLAKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASHITPVPGGVGPMTITMLMEQTYQ
                     AALRTLDRK"
     gene            2588..3460
                     /locus_tag="VVE42_001240"
     CDS             2588..3460
                     /locus_tag="VVE42_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEU3073433.1"
                     /translation="MKVINQILLEKVIIERSRSSHKGDYGRLLLLGGTYPYGGAIIMA
                     ALAAVKSGAGLVTVGTDRENIPALHSHLPEPMAFSLQDQQLLKEQLEKAEVVLLGPGL
                     RDDASGENLVKQVFVNLSQNQILIVDGGALTILARTSLSFPSSQLILTPHQKEWEKLS
                     GITIEKQKEDATASVLTSFPQGTILVEKGPATRIWEVGQSDYYQLQVGGPYQATGGMG
                     DALAGMIAGFVGQFRQASLYERVAVATHLHSAIAQELSQENYVVLPTEISRYLPKIMK
                     IICQQERVSKDKLV"
     gene            3742..4425
                     /gene="rpiA"
                     /locus_tag="VVE42_001241"
     CDS             3742..4425
                     /gene="rpiA"
                     /locus_tag="VVE42_001241"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEU3073434.1"
                     /translation="MENLKKMAGIKAAEFVSDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQITAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRHFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QISTSKKGK"
     gene            4439..5650
                     /locus_tag="VVE42_001242"
     CDS             4439..5650
                     /locus_tag="VVE42_001242"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEU3073435.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLNVPNMAKIGLGNIPRETPLKTVAAESNPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFSPTVLDKLNEAGIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPAFKGNGLIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            5652..6194
                     /locus_tag="VVE42_001243"
     CDS             5652..6194
                     /locus_tag="VVE42_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEU3073436.1"
                     /translation="MTRYALLVRGINVGGKNKVVMAELRQELTNLGLEKVESYINSGN
                     IFFTSIDSKAQLVEKLETFFAVHYPFIQSFSLLSLEDFEAELENLPAWWSRDLARKDF
                     LFYTEGLDVDQVIATVESLELKDEVLYFGKLGIFWGKFSEESYSKTAYHKYLLKVPFY
                     RHITIRNAKTFDKIGQMLKK"
     gene            6210..7019
                     /locus_tag="VVE42_001244"
     CDS             6210..7019
                     /locus_tag="VVE42_001244"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEU3073437.1"
                     /translation="MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVV
                     VDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIRFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTP
                     EYRATAHEVAKKLNIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL"
     gene            7495..7890
                     /locus_tag="VVE42_001245"
     CDS             7495..7890
                     /locus_tag="VVE42_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HEU3073438.1"
                     /translation="MLDSKEELKESENDAPKLETPLREEPRLAPQTLPEASEVLENKR
                     EESKVEITEPAQADDIRKVVGELAKDISITKLYMTGHSLGGYLAQIAAVEDYQKYPDF
                     YNHVLRKVTTFSAPKVITSRTVWDAKNGF"
     gene            7900..8475
                     /locus_tag="VVE42_001246"
     CDS             7900..8475
                     /locus_tag="VVE42_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000445853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEU3073439.1"
                     /translation="MESRKLAVSGKIKHYVVDNDNVVTPLIHNNRDIVTFTGNSRFKH
                     RSRGYFESPMNDIPNFNIGKQATLDKHGYRDPKLDKARFFKKQALPQSSSQPSAEPME
                     NIALGKQVTQSSTAFGGDARRAVDGKVDGNYGHNSVTHTNFQSKPWWQVDLAKEETIR
                     QINIYNRTDTAQDRLANFDVILLDGSGKEIE"
     gene            8500..9012
                     /locus_tag="VVE42_001247"
     CDS             8500..9012
                     /locus_tag="VVE42_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
                     /protein_id="HEU3073440.1"
                     /translation="MSAQITINHKKARYVRIELEGYNALSLAEVEVFRFIATNAETAT
                     QVSKPVQPISQTPVKDKTLTIQHSGAYIARYSITWEEVPVDKDGNQVVRSHSWEGNGR
                     NQTAGFVLNLPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPILAQPHRKITHWGTTL
                     NSKVSDDDVL"
     gene            9666..10376
                     /gene="deoD"
                     /locus_tag="VVE42_001248"
     CDS             9666..10376
                     /gene="deoD"
                     /locus_tag="VVE42_001248"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEU3073441.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLDDAVCFNEVRN
                     MFGYTGTYKGHCVSVMGTGMGMPSISIYARELIVDYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKELGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
     gene            complement(10399..10923)
                     /locus_tag="VVE42_001249"
     CDS             complement(10399..10923)
                     /locus_tag="VVE42_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HEU3073442.1"
                     /translation="MKIAIIGYSGSGKSTLAEKLSNYYSIPKLHMDTLQFQPGWQDSD
                     HEWMLTEIKNFLTKHKAWVIDGNYSWCYYQERMQEADQIIFLNFSPLTCLFRAFKRYL
                     KYRGKVRESMAADCPERFEWEFIRWILWDGRSKTQKENYQKLCQEYSHKVTILRNQRE
                     LDQFLDKKRKSYNS"
     gene            complement(10976..11212)
                     /locus_tag="VVE42_001250"
     CDS             complement(10976..11212)
                     /locus_tag="VVE42_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEU3073443.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSAKLTK"
     gene            complement(11280..12200)
                     /locus_tag="VVE42_001251"
     CDS             complement(11280..12200)
                     /locus_tag="VVE42_001251"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEU3073444.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEVYDIVPEKKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVESFAHQVWTSINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
BASE COUNT         3670 a         2195 c         2795 g         3614 t
ORIGIN      
        1 tagaaagcag tgtgactatg aatttttctt ttttacctaa gtatttacct tattttaact
       61 atggggctgt tgtgacgatt cttatttcta tctgtgttat ctttttggga actattttgg
      121 gtgttgtctt ggcttttggg caacgttcaa agtttaaacc gcttgtttgg ttggccaact
      181 tgtacgtttg gattttccgt gggacaccga tgatggttca aattatgatt gcctttgctc
      241 ttatgcatat caatgctccg actattcaga ttggaatttt aggtgttgat ttttcgcgtc
      301 tgattccagg gattttgatt atctctatga atagtggtgc ttatgtttcg gagactgttc
      361 gtgccggaat caatgcggtt ccaaaaggtc agctagaggc ggcttattcg ctagggattc
      421 gtcctaaaaa tgcgatgcgt tatgtgattt tgccacaagc agtcaaaaat atcttgccag
      481 cattggggaa cgaatttatc accattatca aggacagctc cctcttatca gctattgggg
      541 tcatggagtt gtggaatggg gctacaacag tttctacaac aacctatcta cctttaacac
      601 cacttttatt tgcagcattt tactacttga ttatgacctc tattctgaca gtagccttga
      661 aagcttttga aaaacatatg ggacaaggag ataagaaata atgacagaaa ccttgataaa
      721 aattgaaaat ttacataaat cttttggaaa gaatgaagta ttgaagggca tcaacctcga
      781 gattaaaaga ggagaagttg tcgttatcat cggtccttca gggagtggga aatctacctt
      841 gcttcgctct atgaatttgt tggaggaagc aaccaagggg aagcttatct ttgagggagt
      901 cgatattacg gacaagaaga atgacctgtt tgccatgcgt gagaagatgg gcatggtttt
      961 ccaacaattt aacctctttc ctaatatgac tgtgatggaa aatattacct tgtcacctat
     1021 caagactaaa ggtgaaagca gggaagttgc tgagaagaga gcccaagagc ttttggaaaa
     1081 agttggtttg ccagataagg cagacgctta tccacagagt ctgtcgggtg ggcaacaaca
     1141 acggattgcc atcgcgcgtg ggttggctat ggaaccagat gttttgctct ttgacgaacc
     1201 aacttcagcc ttggatcctg aaatggtagg tgaggtattg gctgttatgc aagaccttgc
     1261 caagtcaggg atgactatgg ttatcgtaac acatgagatg ggatttgccc gtgaggtggc
     1321 agatcgtgtt atctttatgg cagacggtgt ggttgttgaa gatgggacac ctgagcagat
     1381 ttttgaacaa acccaagaac aacggactaa agatttcttg agtaaggttt tataatctat
     1441 tttaaaattt caagacaaga ttagtgaaaa gttcaaccag agctttttct tatagtttga
     1501 aactatagga taacctagga aagaagtgtt agagggcact tgcagttttt ggtataatgg
     1561 aagatatttg aaagaaaaga gaagtgatat gacacagatt attgatggga aagctttagc
     1621 ggccaaattg caggggcagt tggctgaaaa gactgcaaaa ttaaaggaag aaacagctct
     1681 agtgcctggt ttggtagtga ttttggttgg ggataatcca gccagccaag tctacgttcg
     1741 caacaaggag aggtcagctc ttgcggctgg ttcccgtagc gaagtagtgc gagttccaga
     1801 gaccattact caagaggaat tgttagacct gattgctaaa tataatcagg atccagcttg
     1861 gcatgggatt ttggtccagt tgccattacc aaaacatatc gatgaagagg cggttttatt
     1921 agccattgac ccagaaaagg atgtggatgg tttccatccc ctaaacatgg gacgtctttg
     1981 gtctggacat ccagtcatga ttccctcgac acctgcagga attatggaaa tgtttcatga
     2041 atatgggatt gacttggaag gtaaaaatgc ggtcgtaatc ggtcgttcaa atatcgttgg
     2101 aaaacctatg gctcagcttc ttttggctaa aaatgcgaca gtaaccttgg cccactcacg
     2161 aactcataat cttgccaagg tggctgctaa agcagatatt cttgtagtcg caatcggccg
     2221 cgccaagttt gtgactgctg actttgtcaa accaggtgcg gttgtcattg acgttgggat
     2281 gaatcgagat gaaaatggga agctctgtgg agatgttgat tatgaggcag ttgcaccact
     2341 tgctagccat atcacgccag tccctggagg tgtcggtcct atgaccatta ccatgctgat
     2401 ggagcaaact tatcaagcag cacttcggac attggataga aaataagaga aacattcgat
     2461 taatagagtg tattttctat agctatatct aaaatggcag aaatgaatat taaattttag
     2521 atataagatt acaaaaggag gtctgcgtct cctttttatt gtataataaa agtgagagga
     2581 aaaaaggatg aaagtgatta atcaaatctt attagaaaaa gttattattg aacgttctcg
     2641 ttcaagtcat aaaggagatt atggtcgtct gctgctgctt ggtggtactt atccttatgg
     2701 aggtgccatc atcatggctg ctttagcagc tgtaaaaagc ggtgcaggat tggtgaccgt
     2761 tggaacggac agggaaaata tcccggctct gcacagccat ttacctgagc ctatggcctt
     2821 ttctcttcaa gaccagcaat tgttaaaaga gcaattggag aaggcagaag ttgtcttgct
     2881 ggggcctggt ttacgagacg atgcttctgg agaaaatcta gtaaaacagg tctttgttaa
     2941 tttaagccaa aatcagattt tgattgtaga cggaggtgcc ttgaccattc ttgctaggac
     3001 aagtttgtca tttccctcaa gccagcttat cctaactcct caccaaaaag aatgggaaaa
     3061 attatctgga attacgattg aaaagcaaaa ggaagatgca acggctagtg ttctgacttc
     3121 cttccctcaa ggaacaattt tggtggagaa aggcccagct actcgtattt gggaagttgg
     3181 ccaatctgat tattaccagt tacaggttgg tggtccttat caggcgactg ggggaatggg
     3241 agatgctctg gctgggatga tcgcaggatt tgtaggccaa ttccgacagg ccagtctcta
     3301 cgagcgtgtg gcagtagcga cccatcttca ttcagccata gcccaagaac tatctcaaga
     3361 aaattatgtg gtcttaccga cggaaatcag caggtatctt cccaaaataa tgaaaattat
     3421 ttgtcaacaa gagagggtaa gcaaagataa acttgtttaa aatagacaga taaatctact
     3481 tccttctctt aatgtatgat tcttgaaatg cgcctgcttt aaggctaaac tagacgtatc
     3541 aaagttaaga gtttgttttt gtaactggaa agagatgaac aaatcaaata cataaaagat
     3601 aaactcattt ttattcgttt ggtaaaaata aaatgcatat ttttaaagaa agattgatga
     3661 aaaatgtaga tttgatgttt ttacttgaat aaaaagcggt ttcatagtaa aataagaact
     3721 gagaatgatg gaggtaaatc ggtggaaaat ctgaagaaaa tggcaggtat caaggctgct
     3781 gagttcgtga gcgatggaat ggtcgttgga cttggaacag gctcgactgc ctattatttt
     3841 gtcgaagaaa tcggtcgtcg tatcaaggaa gaaggattgc agattacagc tgtaacaact
     3901 tctagtgtga ccactaaaca ggcagaaggc cttaatatcc cgctcaagtc tattgaccaa
     3961 gtagactttg tcgatgtgac ggttgatggc gcggatgaag tggatagtca gtttaatgga
     4021 atcaaaggcg gtggtggtgc ccttctcatg gaaaaggtgg tcgcaacacc atcaaaagaa
     4081 tacatttggg tggtggatga aagcaagctg gtcgaaaaac taggtgcttt taaattgcca
     4141 gtagaagtgg ttcagtatgg tgcagagcag gtctttcgtc attttgaacg agctggctac
     4201 aaaccaagtt tccgtgaaaa agacggccaa cgttttgtga cggatatgca gaattttatc
     4261 attgacctcg ccttggatgt cattgaaaat ccaattgctt ttggacaaga attggaccat
     4321 gtcgttggtg ttgtggagca tggtttattc aaccaaatgg tggataaggt aatcgttgct
     4381 ggacgggatg gagttcagat ttcaacttca aaaaaaggaa aatagaaggg ggcataagat
     4441 gtctaaattt aatcgtattc atttggtggt actggattct gtaggaatcg gtgcagcacc
     4501 agatgctaat aactttgtca atgcaggggt tccagatgga gcttctgaca cactgggaca
     4561 catttcaaaa acagttggtt tgaatgtccc aaacatggct aaaataggtc ttggaaatat
     4621 tcctcgtgaa actcctctta agactgtagc agctgaaagc aatccaactg gatatgcaac
     4681 aaaattagag gaagtatctc ttggtaagga tactatgact ggacactggg aaatcatggg
     4741 actcaacatt actgagcctt tcgatacttt ctggaacgga ttcccagaag aaatcctgac
     4801 aaaaatcgaa gaattctcag gacgcaaggt tattcgtgaa gccaacaaac cttattcagg
     4861 aacggctgtt atcgatgatt ttggaccacg tcagatggaa actggagagt tgattatcta
     4921 tacttcagct gaccctgttt tgcagattgc tgcccacgaa gacattattc ctttggatga
     4981 attgtaccgt atctgtgaat acgctcgttc gattaccctt gagcgtcctg cccttcttgg
     5041 tcgcatcatt gctcgccctt atgtaggtga accaggtaac ttcactcgta cggcaaaccg
     5101 tcgtgacttg gctgtatctc cattttcccc aactgttttg gataaattga atgaggctgg
     5161 tatcgatact tatgctgtgg gtaaaatcaa cgatatcttt aacggtgctg gtatcaacca
     5221 tgacatgggt cacaacaagt caaatagtca tggaattgat acactattga agactatggg
     5281 acttgctgag tttgaaaaag gattctcatt cacaaaccta gttgactttg atgcccttta
     5341 cggccatcgt cgtaatgctc acggttaccg tgattgcttg catgagtttg atgaacgctt
     5401 acctgaaatt atcgcagcta tgagagagaa tgaccttctc ttgattactg cggaccatgg
     5461 aaatgaccca acgtatgcag gaacggatca cactcgggaa tatattccat tgttggccta
     5521 tagccctgcc tttaaaggaa atggtctcat tccagtagga cattttgcag atatttcagc
     5581 gactgttgcc gataactttg gtgtggaaac tgctatgatt ggggaaagtt tcttagataa
     5641 attggtataa gatgacgcgc tatgctttgc tggtgagagg tatcaatgtt ggtggtaaga
     5701 ataaggtcgt catggcggag cttcgtcaag aattgacaaa cttgggactg gaaaaggttg
     5761 agagctacat caacagtggc aatattttct ttacttcgat agattccaaa gcccaattgg
     5821 ttgaaaagct agagactttc tttgcagtcc attatccatt tattcagagc ttttctttac
     5881 tgagtctaga ggactttgag gcggaacttg aaaatctacc agcttggtgg agcagagact
     5941 tggcacgaaa agattttctc ttttacactg agggtttgga tgtggaccaa gtcatcgcga
     6001 cagttgaaag tttagagctg aaagatgaag tgctttattt tggaaaactt gggattttct
     6061 gggggaaatt ttctgaagaa tcctattcta agactgccta tcataagtac ttgctgaagg
     6121 tgcctttcta ccgccacatc actattcgta atgctaaaac ctttgacaaa attggtcaaa
     6181 tgctaaaaaa ataataaagg agacacacaa tgacattttt aaacaaaatc catgaaactg
     6241 ctactttcct gaaagaaaag ggaattgcag cccctgagtt cggtctaatc cttgggtcag
     6301 gacttggaga attggcagaa gaaatcgaaa atccagttgt agtagactat gctgagattc
     6361 caaactgggg ccgttcaaca gtagtcggtc atgctggtaa attggtatat ggtgaactgg
     6421 caggtcgcaa ggtcttggct cttcaagggc gtttccattt ctatgaaggg aatcctctgg
     6481 aagtggtgac tttcccagtt cgtgtgatga aagttcttgg atgtgaaggt gttattgtaa
     6541 ccaatgcagc tggcggtatc agatttggtc ctggtacctt gatggctatc tcagaccata
     6601 tcaacatgac ggggcaaaat ccattgatgg gtgaaaactt ggatgacttt ggcccacgtt
     6661 tcccagatat gtctagggcc tacacaccag aataccgtgc cactgcccat gaagtggcta
     6721 aaaaacttaa tatcaagctt gatgaaggtg tctatatcgg agttactggt ccgacttatg
     6781 aaacaccagc agaaattcgt tcctataaga cactgggagc agatgcagtt ggtatgtcta
     6841 cggttcctga agttatcgtg gcagcccact ctggcttgaa agttctggga atttcatgta
     6901 tcactaactt tgcggctggt ttccaagaag aactcaatca cgaagaagtt gtagaagtga
     6961 ctgaacgtgt taaaggtgat ttcaaaggct tgcttaaagc gattcttgct gaattgtaag
     7021 aaaaaagatt taaaaggggg agtgcctctg tcttttcagg attgactgcc tatccgtatt
     7081 aaagaagaaa cagaggaata ctatgagctt cttcctgctc ttataactga aagaagcgga
     7141 agaataggtg tgtctgatct gatagccagc attgtgaaag acaagattct aggatactag
     7201 cattagcttc ctagccaagc agactagtat gataaggaga gatgagaatg aattgacttt
     7261 ctgaatttct cagtcttatc atatatagca caatgagatt tcgcttgagt ctgcttgtaa
     7321 ataaacgaaa agaaagataa gaaataatga aaattggtca acgaattatg cgctttggca
     7381 taaaaaatta agtatcggag ttgtatctgt tgtagtcggc tttgatttct agctccagct
     7441 ggaatttcag ccaatgaagt aaagcaagat gtaacatctg aagtggtaat aggtgtgcta
     7501 gattctaagg aggaattgaa agagtcagaa aatgatgctc caaaactaga aactcctctt
     7561 agagaggagc caagactagc tcctcaaacg cttccggaag caagtgaagt tcttgaaaac
     7621 aaaagggaag agtcaaaagt agagataaca gagccagctc aagcggatga tatccgcaag
     7681 gttgttgggg aattagccaa ggatataagt attactaagt tgtatatgac aggtcattct
     7741 cttggaggct acctagctca gattgcagcg gttgaagatt accaaaaata tcctgatttt
     7801 tataaccatg tattgaggaa agtgacaact ttcagtgctc ctaaagtgat tacttccaga
     7861 actgtttggg atgctaagaa tggtttctga gatgttggtt tggaaagtcg taaattagct
     7921 gttagtggaa aaattaagca ttatgtggtt gataatgaca atgttgtgac tcccttgatt
     7981 cataataatc gtgatattgt tacatttaca ggtaattcac gctttaaaca ccgttctcgt
     8041 ggctattttg aaagtccaat gaatgatatt cctaacttta atattggtaa acaagctacc
     8101 ttggataaac atggttatcg tgatccgaaa ttggataaag cgcgattctt taagaaacag
     8161 gctctacctc aatcttctag tcaaccaagc gctgaaccaa tggaaaatat tgccttagga
     8221 aaacaggtta ctcaaagttc gacagctttc ggaggagatg ctagaagagc tgtggatggc
     8281 aaagtcgatg gtaactatgg tcacaattct gtcactcata caaacttcca atctaagcct
     8341 tggtggcaag tagatttggc taaagaagaa accattcgcc aaatcaatat ttacaaccga
     8401 acagacactg cccaggatag attggcaaac tttgatgtca ttcttttaga cggttctggt
     8461 aaagaaattg agtgaaaacg tataatatct cctaaagatg tgtcagcaca aattacgatt
     8521 aaccataaaa aagcgcgcta tgttcggatt gagctagaag gctataatgc cctcagtctt
     8581 gcagaagttg aagttttccg ctttatagct acgaatgctg aaacggcgac acaagtttct
     8641 aagccagttc aaccaatcag tcagactcct gtgaaggata aaacattgac aattcaacac
     8701 agtggagctt acattgcccg ctactccata acttgggaag aagttccagt agataaagat
     8761 ggaaaccaag ttgttcgtag tcattcttgg gaaggaaacg gtcgcaacca gactgcaggt
     8821 tttgtcctca acctcccaat caaagaaaat atgagaaatc tgcgagttaa gattgagaaa
     8881 aagacgggcc tactatggaa tagatggcaa acaatctatg aaaacagacc aattttagct
     8941 caaccccacc gtaaaattac ccattggggt acgacattga attccaaggt gagtgacgat
     9001 gatgtcttgt aatctgatgg tacaatgaca gttagtttgt ctagtttata agaaagtact
     9061 acctgagctt gaatagaact caggtagctc tctgtgaaag aacaaaatta atactcaatg
     9121 aaaatcaaag agcaaactag gaaactagcc gcaggttgct caaagcactg ctttgaggtt
     9181 gtagataaga ctgacgaagt cagtcacata tataatccaa ggcgacgttg acgtggtttg
     9241 aagagatttt agaatagtat aaacagaaag gtagagcgcg tgttctaatt tgaacacgag
     9301 tagaaaactt ttctaaaaac aaaaacgaaa ggatgggtaa actgtattcg ctgaactgaa
     9361 tacgggcgac tctcctctaa atcaaaatta agaaaggaat tgaccccacc ctaaaagtag
     9421 tgggaaaaag atagttggtc tagcgagcat cgctcactgc gcccaactcc tattttccct
     9481 tcgctttttg atgggtttgg tatctttctc aatataaaat ataaaataaa gaaaggtaga
     9541 gcgcgtgttc taatttgaac acgagcggaa aactcggaaa atagataatc tgactgaaaa
     9601 atcaggattt ctcgtcaggt tcctaatttt cagtcgtttt cttctcgctc tttgtatcat
     9661 aaattatgtc tatccatatt gctgctcagc agggtgaaat tgctgataaa attcttcttc
     9721 ctggggatcc tcttcgtgct aagtttattg cggagaattt ccttgatgat gctgtttgtt
     9781 ttaacgaagt gcgtaacatg tttggttaca ctggtactta caagggtcac tgtgtatctg
     9841 tcatgggaac tgggatggga atgccatcta tttcgattta tgcgcgtgag ttaatcgtag
     9901 actacggtgt gaagaaattg attcgtgtgg gaactgcagg ttctttgaat gaagaggttc
     9961 atgttcgtga attagttttg gcgcaggcgg ctgcaaccaa ctcaaacatc gttcgtaatg
    10021 actggccaca gtacgatttt ccacaaattg ctagctttga tttgcttgat aaagcctacc
    10081 atatcgccaa agaacttggt atgactactc acgttgggaa cgttttgtca tctgatgtct
    10141 tttactcaaa ttactttgaa aagaatatcg agcttggtaa atggggagtc aaggctgtgg
    10201 aaatggaagc agcagctctt tactatcttg ctgcccaata ccatgttgat gcgctagcta
    10261 tcatgaccat ctctgatagc ttggtcaatc cagacgaaga cacaactgca gaagaacgtc
    10321 aaaatacctt cactgatatg atgaaggttg gtttggaaac cttgattgca gaataattat
    10381 agccaaaaag gaagcccttt atgaattgta ggacttcctt ttcttatcca gaaattgatc
    10441 tagctctctc tgatttcgaa gaatagtgac tttatgtgaa tattcttggc aaagtttttg
    10501 gtaattttct ttttgagttt tgctacgccc atcccaaaga atccatctga taaactccca
    10561 ctcaaagcgt tcagggcaat ctgccgccat actttctctg acttttccac ggtatttaag
    10621 ataacgctta aaggctctaa agagacaggt caatggcgaa aaattgagaa agatgatttg
    10681 gtcagcttct tgcattcgtt cttggtagta gcaccaagaa taattaccat cgatgaccca
    10741 agctttatgc ttggtgagaa agttttttat ctcggttaac atccattcgt ggtcactgtc
    10801 ttgccaacca ggttgaaatt ggagtgtgtc catgtgcagt tttggaatgg agtagtagtt
    10861 agataacttt tctgctagag ttgacttacc agaaccagaa tatccgataa ttgcgatttt
    10921 cattttctac cttttcctat ttggagacaa aaaaacagcc tctatggact gtttcttatt
    10981 tagtaagttt agctgaaaga cgagctttat cgcggcttgc tttgtttttg tgaatcaaac
    11041 ctttagtttc tgctttatcg atagctgagc tagcagcacg gaaaagttct tcagatgggt
    11101 ttgcttcgaa agcttttata gcagtacgca tagctgattt ttgagctgag ttcttttcgt
    11161 tttgtttaac gttcaattca gcgcgtttga tagctgattt aatgtttgcc aatgttctta
    11221 cctccatatt tactaactat accattatat atgaaaactt acgttttgac aagggaaaat
    11281 tactttttta agtaaatttc atcgatttca tggttcttgc ttttatgaag aatcacttct
    11341 gcacgatttc tggttggttc aatataattt tgcagatttg tgagattgat actggtccag
    11401 acctgatggg caaaggactc cacttcccca atcggcatct gagtaaaacg ataatagtag
    11461 ctatcagggt cgttttgggc taggctcagc attttcaaga aacggtccag ataccaactt
    11521 tcaatatcat ccactccagc atctacatag atggaaaagt caaagaagtc agtgatatag
    11581 agacgatcgt tttgtggatt ttgaaagaca ttaattccct caacaattac aaaatcagca
    11641 gctttgacac tttgtttctt ttcgggtacg atgtcgtaaa cttcatgaga atagacagga
    11701 atatctacat cttgtccatt tttgatgcgg tccaagaagt tgagaagagc ttccatatca
    11761 tagctttcag gaaatccttt acgatttaaa atcccctgct caatcaaggt ttgattggga
    11821 tagagaaaac catcagttgt aaccaactca accgtagcat ctgtaaacgt acgggacagt
    11881 aggatttgaa gtaggcgact ggttgtggat tttccaacgg caacactccc agaaacccca
    11941 ataataaaag gttgagattt actttcacgt tggaggaaaa ttccttttga aaaggctaaa
    12001 tcttccttag ttcgcttgta aatctgaatc agatgagcca aggggagata gatatctgta
    12061 acgtcttgga gactgatttg gtcattaaaa ctcttgatag attccaattc ttcttctgtc
    12121 aaaggaggtg ttgtctttcg atgtaaagat tgccaagtct ggcggctgat tttttcaaaa
    12181 tgtaaaaatt cgttggtcat ttgttttccc ctagatattt tgtctatcat accataaaaa
    12241 actaaaaaaa taaagcggtt tcttgaatgt ggta
//
LOCUS       DAVGJL010000057        11864 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.057, whole genome shotgun sequence.
ACCESSION   DAVGJL010000057 DAVGJL010000000
VERSION     DAVGJL010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11864
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.057"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            20..466
                     /gene="argR"
                     /locus_tag="VVE42_001252"
     CDS             20..466
                     /gene="argR"
                     /locus_tag="VVE42_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU3073445.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLVNETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDVNKDEWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            517..3051
                     /gene="mutS"
                     /locus_tag="VVE42_001253"
     CDS             517..3051
                     /gene="mutS"
                     /locus_tag="VVE42_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEU3073446.1"
                     /translation="MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSSKPDSQNNFLVSIDREGNQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSRQMNLVLSYEKESFEDLHLLDLRLATVEQT
                     ASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPTLAYLIAQLDAIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSA
                     NLEYEIFMRIREEVGKYIQRLQALAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IRKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLITGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTESPPPMRQT
                     SAVTEQISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            3198..4892
                     /gene="patA"
                     /locus_tag="VVE42_001254"
     CDS             3198..4892
                     /gene="patA"
                     /locus_tag="VVE42_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatA"
                     /protein_id="HEU3073447.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLGALLTG
                     KYEAIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGMMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTDV
                     SDELLGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDIPDEELVGSLSFENVTF
                     TYPMDKEPMLKDVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQDGAIKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
     gene            complement(5042..5485)
                     /locus_tag="VVE42_001255"
                     /pseudo
     CDS             complement(5042..5485)
                     /locus_tag="VVE42_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844241.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            5683..7449
                     /gene="patB"
                     /locus_tag="VVE42_001256"
     CDS             5683..7449
                     /gene="patB"
                     /locus_tag="VVE42_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HEU3073448.1"
                     /translation="MKTVQFFWHYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAYQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTHVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNIVLYIGLILV
                     MFSRNVTLALITIASTPLAFLMLIFIVKMARKYTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPVMNGMGLINTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEISHIDFSYLPDKPILKDVSISAPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVPDA
                     SQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSTGQKQLISIARTLMTDPEVLI
                     LDEATSNVDTVTESKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFE"
     gene            7588..8277
                     /locus_tag="VVE42_001257"
     CDS             7588..8277
                     /locus_tag="VVE42_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEU3073449.1"
                     /translation="MARTVVGVAANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLNEL"
     gene            8281..8460
                     /locus_tag="VVE42_001258"
                     /pseudo
     CDS             8281..8460
                     /locus_tag="VVE42_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866816.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8447..9796
                     /locus_tag="VVE42_001259"
     CDS             8447..9796
                     /locus_tag="VVE42_001259"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEU3073450.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWGTFVVPDDIGGRFSVLTAVGLL
                     PIAASGADIKALMEGANAARKDYTSDKISENEAYQYAAVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVLIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
     gene            9920..11380
                     /locus_tag="VVE42_001260"
     CDS             9920..11380
                     /locus_tag="VVE42_001260"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEU3073451.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWIIQKKDGYPTYNFAVAIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQIKSVDEIIPLTDLFFSDFPELTEA
                     EREVMTGETVPTVLEAFKAKLEAMTDDKFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENMLKEISK"
     gene            11394..11828
                     /locus_tag="VVE42_001261"
     CDS             11394..11828
                     /locus_tag="VVE42_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEU3073452.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAEDGQIFTGF
                     CMEGTCGVFHLCAERAALFNMYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACREFLL
                     ELNAENKDAEFMMDYNIRKTVKVAELIPYWWGKERASKFNER"
BASE COUNT         3508 a         2305 c         2700 g         3351 t
ORIGIN      
        1 atgcacgcaa gaggatttta tgagaaaaag agatcgtcat cagttaataa aaaaaatgat
       61 tactgaggag aaattaagta cacaaaaaga aattcaagat cggttggagg cgcacaatgt
      121 ttgtgtgacg cagacaacct tgtctcgtga tttgcgcgaa atcggcttga ccaaggtcaa
      181 gaaaaatgat atggtgtatt atgtactagt aaatgagaca gaaaagattg atttggtgga
      241 atttttgtct catcatttag aaggtgttgc aagagcagag tttaccttgg tgcttcatac
      301 caaattggga gaagcctctg ttttggcaaa tattgtagat gtaaacaagg atgaatggat
      361 tttaggaaca gttgctggtg ccaatacctt attggttatt tgtcgagatc agcacgttgc
      421 caaactcatg gaagatcgtt tgctagattt gatgaaagat aagtaaggtc ttgggagttg
      481 ctctcaagac ttatttttga aaaggagaga cagaaaatgg cgatagaaaa gctatcaccc
      541 ggcatgcaac agtatgtgga tattaaaaag caatatccag atgctttttt gctctttcgg
      601 atgggtgatt tttatgaatt attttatgag gatgcggtca atgctgcgca gattctggaa
      661 atttccttaa cgagtcgcaa caagaatgcc gacaatccga tccctatggc gggtgttccc
      721 tatcattctg cccaacagta tatcgatgtc ttgattgagc agggttataa ggtggctatc
      781 gcagagcaga tggaagatcc taaacaagca gttggggttg ttaaacgaga ggttgttcag
      841 gtcattacgc cagggacagt ggtcgatagc agtaagccgg acagtcagaa taattttttg
      901 gtttccatag accgcgaagg caatcaattt ggcctagctt atatggattt ggtgacgggt
      961 gacttttatg tgacaggtct tttggatttc acgctggttt gtggggaaat ccgtaacctc
     1021 aaggctcgag aagtggtgtt gggttatgac ttgtctgagg aagaagaaca aatcctcagc
     1081 cgccagatga atctggtact ctcttatgaa aaagaaagct ttgaagacct tcatttattg
     1141 gatttgcgat tggcaacggt ggagcaaacg gcatctagta agctgctcca gtatgttcat
     1201 cggactcaga tgagggaatt gaaccacctc aaacctgtta tccgctacga aattaaggat
     1261 ttcttgcaga tggattatgc gaccaaggct agtctggatt tggttgagaa tgctcgctca
     1321 ggtaagaaac aaggcagtct tttctggctt ttggatgaaa ccaaaacggc tatggggatg
     1381 cgtctcttgc gttcttggat tcatcgcccc ttgattgata aggaacgaat cgtccaacgt
     1441 caagaagtag tgcaggtctt tctcgaccat ttctttgagc gtagtgactt gacagacagt
     1501 ctcaagggtg tttatgacat tgagcgcttg gctagtcgtg tttcttttgg caaaaccaat
     1561 ccaaaggatc tcttgcagtt ggcgactacc ttgtctagtg tgccacggat tcgtgcgatt
     1621 ttagaaggga tggagcaacc tactctagcc tatctcatcg cacaactgga tgcaatccct
     1681 gagttggaga gtttgattag cgcagcgatt gctcctgaag ctcctcatgt gattacagat
     1741 gggggaatta tccgaactgg atttgatgag actttagaca agtatcgttg cgttctcaga
     1801 gaagggacta gctggattgc tgagattgag gctaaggagc gagaaaactc tggtatcagc
     1861 acgctcaaga ttgactacaa taaaaaggat ggatactatt ttcatgtgac caattcgcaa
     1921 ctaggaaatg tgccagccca ctttttccgc aaggcgacgc tgaaaaactc agaacgcttt
     1981 ggaaccgaag aattagcccg tatcgaggga gatatgcttg aggcgcgtga gaagtcagcc
     2041 aacctcgaat acgaaatatt tatgcgcatt cgtgaagagg tcggcaagta catccagcgt
     2101 ttacaagctc tagcccaagg aattgcgacg gttgatgtct tacagagtct ggcggttgtg
     2161 gctgaaaccc agcatttgat tcgacctgag tttggtgacg attcacaaat tgatatccgg
     2221 aaagggcgcc atgctgtcgt tgaaaaggtt atgggggctc agacctatat tccaaatacg
     2281 attcagatgg cagaagatac cagtattcaa ctgattacag ggccaaacat gagtgggaag
     2341 tctacctata tgcgtcagtt agccatgacg gcggttatgg cccagctggg ttcctatgtt
     2401 cctgctgaaa gcgcccattt accgattttt gatgcgattt ttacccgtat cggagcagca
     2461 gatgacttgg tttcgggtca gtcgaccttt atggtggaga tgatggaggc caataatgcc
     2521 atttcgcatg cgaccaagaa ctctctcatt ctctttgatg aattgggacg tggaactgca
     2581 acttatgacg ggatggctct tgctcagtcc atcatcgaat atatccatga gcacatcgga
     2641 gctaagaccc tctttgcgac ccactaccat gagttgacta gtctggagtc tagtttacaa
     2701 cacttggtca atgtccacgt ggcaactctg gagcaggatg ggcaggttac cttccttcac
     2761 aagattgaac cgggaccagc tgataaatcc tacggtatcc atgttgccaa gattgctggc
     2821 ttgccagcag accttttagc aagggcggat aagattttga ctcagctaga gaatcaagga
     2881 acagagagtc ctcctcccat gagacaaact agtgctgtca ctgaacagat ttcactcttt
     2941 gatagggcag aagagcatcc tatcctagca gaattagcta aactggatgt gtataatatg
     3001 acacctatgc aggttatgaa tgtcttagta gagttaaaac agaaactata aaaccaagac
     3061 tcactagtta atctagctgt atcaaggaga cttctttgac aattctccac ttttttgcta
     3121 gaataacatc acacaaacag aatgaaaagg agctgacgca ttgtcgctcc cttttgtcta
     3181 ttttttaagg agaaagtatg ctgattcaga aaataaaaac ctacaagtgg caggccctgg
     3241 cttcgctcct gatgacaggc ttgatggttg ctagttcact tctgcaaccg cgttatctgc
     3301 aggaagtctt aggcgccctc cttactggga aatatgaagc tatttatagt atcggggctt
     3361 ggttgattgg tgtggccgta gtcggtctag ttgctggtgg actcaatgtt gtcctcgcag
     3421 cctatattgc ccaaggagtt tcatccgacc ttcgggagga tgccttccgt aaaattcaaa
     3481 ccttttctta tgctgatatt gaacaattta atgcgggaaa tctagtcgtt cgaatgacaa
     3541 atgatatcaa ccagattcag aacgttgtca tgatgacctt ccaaattctt ttcagacttc
     3601 ccctcttgtt catcggttcg tttatcctag cggttcaaac cttaccttct ctgtggtggg
     3661 tgattgttct catggtagtc ttgatttttg gtttgactgc tgtcatgatg ggaatgatgg
     3721 ggcctcgttt tgctaaattt caaacccttc ttgagcgcat caatgccatt gccaaggaaa
     3781 atttacgtgg cgttcgtgtg gtcaagtcct ttgtccaaga aaaagagcaa tttgctaagt
     3841 ttacagatgt ctcagacgag cttcttggtc aaaaccttta cattggttat gccttttcag
     3901 tagtggaacc ctttatgatg ttggttggtt acggggcggt cttcctctct atttggctgg
     3961 tcgcgggaat ggttcagtcg gatccgtctg ttgttggttc catcgcttct tttgttaatt
     4021 acctaagcca gattatcttt accattgtta tggttggatt tttgggaaat tctgtcagcc
     4081 gtgccatgat ttccatgcgt cgtattcgag aaattcttga cgcagagcca gctatgacct
     4141 tcaaggatat cccagatgaa gagttggttg gaagccttag ctttgaaaat gtgaccttta
     4201 cctatccaat ggacaaggaa ccgatgctga aagatgtgag ctttactatt gaacctggtc
     4261 aaatggttgg tgtagttgga gcgactggtg caggaaagtc aactttggct caattgattc
     4321 cacgtctctt tgatccacag gacggggcca ttaaaatcgg tggcaaggat attcgagaag
     4381 tgagtgaagg aaccctgcgt aaaacagttt ccatcgttct ccaacgtgcc attcttttta
     4441 gtggaacgat tgcagataac ttgagacagg ggaagggaaa tgctactcta tttgaaatgg
     4501 agcgcgcagc caatattgcc caggctagtg aattcattca tcgtatggag aaaacctttg
     4561 aaagtccagt tgaagaacgg ggaaccaatt tctctggtgg acaaaaacaa aggatgtcga
     4621 ttgcgcgtgg gattgtcagc aatccacgta ttctgatttt tgatgattcg acctcagcct
     4681 tggatgccaa atcagagcgc ttggtgcaag aagctttgaa taaggacttg aagggtacga
     4741 caaccattat tattgctcaa aaaattagct cggttgtcca tgcagacaag atcttggttc
     4801 taaatcaagg acgattgatt ggtcaaggca cgcatgcaga cttggttgcc aacaatgccg
     4861 tttaccgtga aatctatgaa acacagaaat gaaagacaaa ctataagaaa agtcaatagt
     4921 tttatctaaa ctatttctta tttcaatttg atgatttggc gatgatttta gagcacggca
     4981 aaaagccctt gaaaaagtcc attttttcaa aggtaatcct gtgttgattt cagaaattac
     5041 atcacttttt gttcgtcaaa tggcagctct tttttaggat ataaaacagg gttcggataa
     5101 gtttttttgc aaggtggatg atggctacat tgtaatgttt tccttgttct aacttagtct
     5161 taagataggc cttaaaagca ggtgaaaagc gagcgcatgc tttggcagct tgtatgagtg
     5221 cccaccgcag atgaggggaa ccccgtttga tcattcttcc agctaaatca atctgacctg
     5281 actgataaat agaagaatcc agtccagcga aagcttgtaa ttgagcagga ttatcaaagg
     5341 catgaatatt tcgaatctcg gctaaaatga ccgcccctaa acgaccccca atcccagtaa
     5401 ccgtcgtgat gactgagttg aactcagcca tcaagtcatt gacacatgtt tccgccttgt
     5461 caatgagcct cttgtaatgt ttgatgtttt cattacacga gataaaacgt ctatgcgtta
     5521 tcaaactcat taccaattaa aacaaaaagc tgtggttaga tcctttcgga aatcgtcaag
     5581 cgattggagg aaatgaacta atccacagcg gcttattcca agtataccac ttgggctttg
     5641 gcagtagcta actgcgctaa atataatata aggaggagta aaatgaagac agttcaattt
     5701 ttttggcatt attttaaggt ctacaagttc tcatttgtag ttgtcatcct gatgattgtt
     5761 ctggcgactt ttgcccaagc cctctttcca gtcttttctg gacaagcggt gacgcagcta
     5821 gccaatttag ttcaagctta tcaaaatggc aatccagaac ttgtatggca aagcctatca
     5881 ggaatcatgg tcaatcttgg cctgctggtt ttggttctat ttatctctag tgtaatatac
     5941 atgtgtctca tgacgcacgt gattgcagaa tcgaccaacg agatgcgcaa aggcctcttt
     6001 ggtaagcttg ctcagttgac ggtttctttc tttgaccgtc gacaagatgg cgatatcctg
     6061 tctcatttta ccagtgattt ggataatatc ctccaagcct ttaacgaaag cttgattcag
     6121 gtcatgagca atattgtttt atacattggt ctgattcttg tcatgttttc gagaaatgtg
     6181 acgctggctc tcatcaccat tgccagcacc ccattggctt tccttatgct gattttcatc
     6241 gtgaaaatgg cacgcaaata caccaacctc cagcagaaag aggtagggaa gctcaacgcc
     6301 tatatggatg agagcatctc aggccaaaaa gccgtgattg tgcaaggaat tcaagaggat
     6361 atgatggcag gatttcttga acaaaatgag cgcgtgcgca aggcaacctt taaaggaaga
     6421 atgttctcag gaattctttt ccctgtcatg aatgggatgg gcctgattaa tacagccatc
     6481 gtcatctttg ctggttcggc tgtacttttg aatgataagt ctattgaaac aagtacagcc
     6541 ctaggtttga ttgttatgtt tgcacaattt tcacagcagt actaccagcc tattatccaa
     6601 gttgcagcga gttggggaag ccttcagttg gcctttactg gagctgaacg aattcaggaa
     6661 atgtttgatg cagaggagga aatccgacct gaaaaggctc caaccttcac taagttgcaa
     6721 gaaagtgttg aaatcagtca tatcgatttt tcatacttgc ctgataaacc tattttgaaa
     6781 gatgtcagca tttctgcccc taaaggccag atgacagcag ttgttgggcc gacaggttca
     6841 ggaaaaacga ctattatgaa cctcatcaat cgcttttatg atgttgatgc tggtggtatt
     6901 tattttgatg gtaaagacat tcgtggctat gacttagata gtcttagaag caaggtggga
     6961 attgtattgc aagattcggt cttgtttagc ggaacgattc gagacaatat ccgatttggt
     7021 gtgccagatg ctagtcagga aatggttgag gtagcagcaa aagcaaccca cattcacgac
     7081 tatatcgaaa gtttgcctga taagtacgat actcttattg atgatgacca gagcatcttt
     7141 tcaacagggc agaagcaatt gatttcaatc gctcgaaccc tgatgacaga tccagaagtt
     7201 ctcattctcg atgaagcaac ttcaaacgta gatacggtga cagaaagcaa gattcagcat
     7261 gccatggagg tggttgtagc aggtagaact agtttcgtca ttgcccaccg cttgaaaacc
     7321 attctcaatg cagatcagat tattgtcctt aaagatggag aagtcattga acgtggtaac
     7381 caccatgaac ttttgaagct aggtggcttt tattcagaac tctatcacaa tcaatttgtt
     7441 ttcgaataag aaagaagttg tcctatgtgg gcagcttttt cttgtccata aaaaatgttt
     7501 atcacagcct taaaaaaaac atattagacg aaagtcattt tgagtgatat gataggacta
     7561 tcgttagcat tcgaaaggag aggcatcatg gctagaacgg ttgtaggagt tgctgcaaat
     7621 ctatgtcccg tagacgcaga aggcaaaatc attcattcat ctgtatcttg tagattcgca
     7681 gagatcattc gtcaagtcgg tggtctccct ttagtcattc ctgttggtga tgagtcagtt
     7741 gtacgtgatt atgtggaaat gattgacaaa ctcattttga caggaggcca aaatgttcat
     7801 cctcagtttt atggagagaa aaagaccgtc gaaagcgatg attacaatct ggtccgtgac
     7861 gaatttgaat tggcactctt gaaggaagcg cttcgtcaga ataaaccaat tatggcaatc
     7921 tgtcgcggtg tccaacttgt caatgttgcc tttggtggaa ccctcaatca agaaatcgaa
     7981 ggtcactggc aaggactacc tttcgggaca tctcactcta ttgagacagt agaaggaagc
     8041 gtggtggcta agctatttgg gaaagaaagt caggtcaact cagtccatcg tcaaagcatt
     8101 aaagatttgg cacctaattt ccgtgtaact gccattgatt caagagacca aactattgaa
     8161 gcgattgagt ctatcgacga gcaccgcatt atcggtttac agtggcatcc agagtttctg
     8221 gttaatgaag aagatggcaa tttagaatta tttgagtatt tattgaatga actgtaacga
     8281 ttggaacatc tggtcgttct ttcttttatt ttttcaaaat ttttagaatg tggcattttt
     8341 acgcaaacgt ttgaattctg ataaaaattg gagaaattcg acaaaaaact tgaaaaaaac
     8401 gaaggtaagc gttatgatag aaaagaagaa atattggagg aagaacatgt cacatattaa
     8461 atttgattat tcaaaagttt tagacaaatt tgttgcacca catgaagtgg aatacatgca
     8521 atcacaagta acagcagcag atgaattgat ccgtaaagga actggtgctg gtagcgactt
     8581 cttgggatgg ttggaccttc ctgaaaaata cgatcgcgaa gaatttgacc gcatcttgaa
     8641 agctgctgag caaatcaagt cagacagcga tgttttggtt gtgatcggta ttggtggatc
     8701 ttaccttggt gcaaaagcag caatcgactt cttgaatcac cattttgcta acttgcaaac
     8761 aaaagaagaa cgcaaagctc cacaaatcct ttacgcagga aactcaatct catctaccta
     8821 ccttgctgac ttggtagagt atgttgcaga caaggacttc tcagtaaacg taatttctaa
     8881 atcaggtaca acaactgaac cagcgattgc tttccgtgtc tttaaagaac tcttggttaa
     8941 gaaatacggt caagaagaag ctaacaaacg tatctatgca acaactgacc gccaaaaggg
     9001 tgctgttaag gttgaagcag acgctaacgg ttggggaaca tttgttgttc cagatgatat
     9061 cggtggacgc ttctcagtat tgacagccgt tggtttgctt ccaatcgcag catcaggagc
     9121 tgacatcaaa gctcttatgg aaggtgcgaa tgcagctcgc aaagactaca cttcagacaa
     9181 aatctctgaa aacgaagctt accaatacgc agctgttcgt aacatccttt atcgtaaagg
     9241 ctatgcaact gagatcttgg taaactatga gccatcactt caatacttct cagaatggtg
     9301 gaaacaattg gctggtgaat cagaaggaaa agaccaaaaa ggtatctacc caacttcagc
     9361 caacttctca actgacttgc actcacttgg tcaatttatc caagaaggaa ctcgtatcat
     9421 gtttgaaaca gttgtccgtg ttgacaaacc tcgtaaaaac gtgcttattc ctactttgga
     9481 agaagacctt gacggacttg gttaccttca aggaaaagac gttgactttg taaacaaaaa
     9541 agcaactgac ggtgttcttc ttgcccacac agatggtgat gtaccaaaca tgtatgtgac
     9601 tcttccagag caagacgctt tcactcttgg ttacactatc tacttcttcg aattggcaat
     9661 tgccctttca ggttacttga atgctatcaa cccatttgac caaccaggtg ttgaagctta
     9721 taaacgtaac atgtttgccc ttcttggaaa accaggattt gaagaattga gcaaagaact
     9781 taacgcacgt ctataataga agaaaagagt ggtttgccca ctctttttac tctctttatc
     9841 catagaaatt ggactcagcc aagacttgtg atataatata gaaagcaaaa aggcagacgc
     9901 ctagataata ggagaaacta tgtcaaaaga tatccgcgta cgttacgcac caagtccaac
     9961 aggactacta cacatcggaa atgctcgtac agcattgttt aattacttgt atgcgcgcca
    10021 tcatggtgga acatttctca tccgtatcga agatactgac cgtaaacgcc atgtcgagga
    10081 tggtgaacgt tcacaacttg aaaaccttcg ctggttaggc atggattggg atgaaagtcc
    10141 agaatcacat gagaattatc gccagtctga gcgtttggac ttgtatcaaa aatacattga
    10201 ccaactatta gctgaaggaa aagcctataa atcttacgtt acagaagaag agttggcagc
    10261 tgaacgcgaa cgccaagaag tagctggcga aacaccacgc tacatcaatg aataccttgg
    10321 tatgagtgaa gaagaaaaag cagcttacat cgcagaacgt gaagcagcag ggatcatccc
    10381 aactgttcgt ttggctgtca atgagtcagg tatctacaag tggcatgata tggtcaaagg
    10441 cgatatcgaa tttgaaggtg gcaatatcgg tggtgactgg attatccaaa agaaagacgg
    10501 ttacccaact tacaactttg ccgttgccat cgatgaccac gatatgcaaa tctctcatgt
    10561 tatccgtgga gatgaccata ttgctaatac accaaaacag cttatggtct atgaagctct
    10621 tggttgggaa gctccagagt tcggtcacat gaccttgatt atcaactctg agactggtaa
    10681 gaagttatct aaacgtgata ctaatacact tcaatttatc gaagattacc gaaagaaagg
    10741 ttaccttcca gaagcagtct ttaactttat tgctcttctt ggttggaacc caggtggtga
    10801 agatgagatt ttctctcgtg aagaactcat taaacttttt gatgaaaacc gcctcagcaa
    10861 gtcaccagca gcctttgatc agaaaaaact agactggatg agcaatgatt atatcaagaa
    10921 tgcagacctt gaaaccatct ttgaaatggc aaaaccattc ttagaggaag caggccgttt
    10981 gactgacaag gctgaaaaat tagttgagct ctataaacca caaataaaat cagtagatga
    11041 gattatccca ttgacagatc ttttcttctc agatttccca gaattgacag aagcagagcg
    11101 cgaagtcatg acgggtgaaa cagttccaac agttcttgaa gcattcaaag caaaacttga
    11161 agcgatgaca gatgataaat ttgtgacaga aaatatcttc ccacaaatta aagcagtcca
    11221 aaaagaaaca ggtattaaag ggaaaaatct tttcatgcct attcgtatcg cagtttcagg
    11281 cgaaatgcat gggccagaat taccagatac aattttcttg cttggacgtg aaaaatcaat
    11341 tcagcatatc gaaaacatgc taaaagaaat ctctaaataa agaggataca ataatggaca
    11401 tctgggaaaa gatgtacgaa gaagcacaga aactatacaa tccacatgaa gtatcagact
    11461 ttgtttatgc caatcatgtt gtagctgcag tagaagcaga agatggacaa atatttacag
    11521 gattctgtat ggagggaacc tgtggtgttt tccatctctg cgcagaacgg gcggccctct
    11581 tcaatatgta ccaattttca ggacaaacta aggttaagaa agtattagcc tttcgagaca
    11641 aaccacctta tggtggaagt tcagccatgc cttgcggtgc ttgtagagaa ttccttttag
    11701 agcttaatgc tgaaaataaa gatgcagaat tcatgatgga ctataatata agaaaaacag
    11761 ttaaagtcgc agaactaatc ccttattggt ggggaaaaga acgtgcttct aagtttaatg
    11821 agcgatagaa gagtcagtat aattctggct ctttttacta taag
//
LOCUS       DAVGJL010000058        11848 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.058, whole genome shotgun sequence.
ACCESSION   DAVGJL010000058 DAVGJL010000000
VERSION     DAVGJL010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11848
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.058"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(61..510)
                     /locus_tag="VVE42_001262"
     CDS             complement(61..510)
                     /locus_tag="VVE42_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEU3073453.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYHYIKTHRVS"
     gene            complement(512..1270)
                     /locus_tag="VVE42_001263"
     CDS             complement(512..1270)
                     /locus_tag="VVE42_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEU3073454.1"
                     /translation="MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAQPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            complement(1385..3364)
                     /gene="pknB"
                     /locus_tag="VVE42_001264"
     CDS             complement(1385..3364)
                     /gene="pknB"
                     /locus_tag="VVE42_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEU3073455.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKNPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSAE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            complement(3361..4101)
                     /locus_tag="VVE42_001265"
     CDS             complement(3361..4101)
                     /locus_tag="VVE42_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEU3073456.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIIDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVSMNEEDAE"
     gene            complement(4116..5429)
                     /gene="rsmB"
                     /locus_tag="VVE42_001266"
     CDS             complement(4116..5429)
                     /gene="rsmB"
                     /locus_tag="VVE42_001266"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEU3073457.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLSAADKG
                     LVTELVYGTVARKLTLEWYLSHFIEDRDQLDSWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNAVLRRILREGWPDIASIKRKNKRDSIAYSLPVWLVTKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANNSLLATTGLVKEQGHFAG
                     HNLFANGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGVADRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            complement(5422..6357)
                     /locus_tag="VVE42_001267"
     CDS             complement(5422..6357)
                     /locus_tag="VVE42_001267"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEU3073458.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEDLMKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGALFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPEEEKLDWNKTN
                     RQLFNQIRGMNPWPVAHTFLKGDRFKIYEALPVEGKGNPGEILSICKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            complement(6370..8766)
                     /locus_tag="VVE42_001268"
     CDS             complement(6370..8766)
                     /locus_tag="VVE42_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEU3073459.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVLGLESQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKTMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLAKQAEMMRLTRK
                     GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGAVTIVQKEVQRSAAYFEGIEASRPLELNPEQRQVRDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEAAYK
                     QDSNPRYHAREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQVVLMLNRRGYSSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSRSIRYYGTGTQKAYDEL
                     AELFPQARILRMDVDTTRKKGSHQALLDQFGRGKADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRAGRAEKAGQVLIQSYNSQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKRAYEVMNILRSGLSETSPILG
                     PTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            complement(8832..9146)
                     /locus_tag="VVE42_001269"
     CDS             complement(8832..9146)
                     /locus_tag="VVE42_001269"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEU3073460.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            complement(9171..9797)
                     /gene="gmk"
                     /locus_tag="VVE42_001270"
     CDS             complement(9171..9797)
                     /gene="gmk"
                     /locus_tag="VVE42_001270"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEU3073461.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVPLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            complement(9927..11540)
                     /locus_tag="VVE42_001271"
     CDS             complement(9927..11540)
                     /locus_tag="VVE42_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEU3073462.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSQEAAELMLLNAEQ
                     EATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LERIGALSQAEARDIILAQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
BASE COUNT         3149 a         2827 c         2366 g         3506 t
ORIGIN      
        1 agaaagcgtt gttaatttat ttatctcatt tttcagaaaa ttcttttatt tctgtaaagt
       61 ctacgatact cgatgtgttt ttatataatg atacagtctg agaatcactg ttccgctagc
      121 cattccaata gagattacca gagccaacat gacaaccaag gtcgcacttg ccagtgcttt
      181 attatagtcc cctgtcacaa aaaaggcagt tgttcggtag gagagataac ctggaaccag
      241 cggtgccaaa atggccaaga taaagaccac agcaggtgtc ttataaagaa tacttaaaat
      301 ctggctgaca caagaaccaa taatggctgc aatgaaggta gctacaatga cattggtcgg
      361 ttccttgagc aagagataga ttagccagac agtcatgccc aaaatccctc caggcaagag
      421 catagaccgt tgcacattga gtacgattaa aaaagtgata atggcaagaa aacttgctac
      481 tgcttgtaat aaaaaggttg ttagtgtcat attagttcat caataccaag gcgacagaag
      541 ttcctgcccc taaagcgagg gtaatgagca gggattcaaa catcttactc ataccagagt
      601 ttatgtggtt ggtcataata tcacggaccg cattggtcaa ggcaatacct ggtacaaacg
      661 gcatgaccgc accagctata atcaaatctg ccgttgaagg aaaacctgtg tagcgagccc
      721 aaaactgggc aattatccca aagacaaagg ctccagcaaa ggctgtcaca aagggaattc
      781 ggataaattt ttccacatag agggaaaagg caaaaccaaa taaggtcgcc actcctgccc
      841 caagtgcgtc gtagatattt ccgctaaaca taactgaaaa gaaaggagca ctaaaggtcg
      901 cagccagagt tacctgcaac ttagtatagg gaaggggttg agcttgcaag gccgtcaatt
      961 gcttaaaggc tgtttctaag tcaatctgcc ccccaactag ctgacgagaa atctggttca
     1021 catcgcagac tttttcaatg ttataagaag aggaggtcac gcgcttcatg cgcgaaatat
     1081 tggtattttc aatagagaaa aagatagcgg caggcatggc aaggacattg caatccacaa
     1141 tcccctgcga atgcgcgatt cgaatcatgg tatcttctac acgatggatt tctgagccac
     1201 ttttaaggag aatagtcccc gctagcataa tcacatcaat gacggcattt aattcttttg
     1261 attcttccat gctttcctcc ttttatcaac tccctctatt ctatcacaaa tccggactca
     1321 aaaaaaatct ttgccatgaa atcatgacaa agattgatta ctcattttga ttatccatct
     1381 gcttttaagg agtagctgaa gttgttttag gtttgtagat tgaaatcttg actctagtct
     1441 tattgaggtc taccttttca cctgctctag gactttgttc aacaaccatg ccttctgcac
     1501 tacctgcagg cgctgtcgtc acttctacaa cttctatatt agcttcctta atcccaacaa
     1561 tttgaatcaa attgttctta gtaaactcca agctggaacc aatgtaactc ggcatggcaa
     1621 cacttgtaac ttttttagct actgtcaaga caatttgagt aggtttactc acatcataag
     1681 tcgttccggc acctggactt tgtttcataa tcgttcctgg ttcgctttcg ctggactctt
     1741 cttcctctat cttaatcaaa ttctcaggaa ccttcttctg cttgagttct gagattactt
     1801 ctgtagagtt ccgtccaata tagttcccta attgaatcgt cgtagctttt ttagctactg
     1861 tcaaaacaat ttgagttgcc ttactcaagt cataggtcgt accttctggt agactttgct
     1921 tcaggaccgt tccagcctca ctctcattgg actcttcttc ctcaatttta atcaaattat
     1981 ctggaacttt tttctctttt aattccgcaa tgacatcaga ggatttccgg ccgacataat
     2041 tactaatttg gaaggattgt ttgcctgatg agacaaccaa attgattttc gttccttctt
     2101 ttcgaccagt tccagcgcca ggatctgtac ggataatccg cccttcttcc accttttcac
     2161 tagcctctgt cttctcctca ccaatctcaa aattggcttt tttgagcgtt gccttggcct
     2221 ctgcaactgt ctgacctgcc acatctggaa tggcaatggt tgcaggagtt ctggatagta
     2281 tccaaataag agaagctgcc accaatacaa ggctggccaa caaaatcagg taacgcatct
     2341 taaatctatg ttttttcggt gcttgtggtt ggtaagtttc ctctgtcaca gcctggcttg
     2401 ggtttttgat tgatttgtgt tctgtctgcg cttgaacctt aggaatagat gtcaaggtac
     2461 tctgagaaac cttcggcaag gtcttggtat ctgccttgct cgtttcatca aagattaact
     2521 tactttcatt tctacgattg taggacaagc tactagacaa gtccacatac atctctgaaa
     2581 ccgagcggta gcgattggtc aactttttag cagttgcctt gataataaca ttttctaaag
     2641 cctgaggtac agatggattt tctgcaataa cggacggcag gggtttctgg aaatgctgga
     2701 gggcaatggt caccgcgcta tccccgtcat aagggatatg gcctgtcagc atctcataga
     2761 aaataatccc catggcatag atatcactct gcacagtcgc cttcgaacca cgcgcctgct
     2821 ctggtgacaa gtaatgaact gagcccaaca tcgagttagt ctgggtcaga cttgtctctg
     2881 caaaggctac agcaatccca aagtctgtga ccttggcagt cccatctggt gtcaaaagga
     2941 tattttgagg tttcaagtcc ctgtgaacaa ttcctctggt atgggccaaa cgcatggcta
     3001 ggaggatttg tcccatgata cggactgctt cttcattaga aagaggataa tgttccttga
     3061 tatagcgttt gaggtctagt ccagcaacat actccattgc aagatactgt tgaccgtctt
     3121 cctcaccaat atctgttatc cgaacgatat gaggatggtc tagatctgcc atagctctcg
     3181 cttcacgctg aaaacgagct acagctatcg ggtccgtctg gtagttggtc ctcagaacct
     3241 tcactgccac ttcttcccca tctaagatta agtctttggc taggtagaca tccgccatac
     3301 ctcctcgacc aatctgtttg acaatccgat agcgtccggc aaaaatcttg ccgatttgga
     3361 tcattctgca tcctcctcgt tcatagaaac aagggcaacc gtaatgttgt ctaaacctcc
     3421 tgcattgtta gcaaaacgaa caagtgtctc cgttttatct gctaaaggaa tatcactggt
     3481 tacaatatca cgaatctcac tgcctgaaat catgttggtc aagccgtcac tattgagcaa
     3541 gagatagtca cctgactcaa ggataactgt cccaaaatca ggctgaattt catctttttg
     3601 cccaatagac tgggtgataa tatttttttg cggatgagct tctgcctctt ctggtgtcaa
     3661 ttgaccagcc ttgagcaatt cattaaccaa agaatgatcg ctcgtcaact gatggtattc
     3721 ttctccacga atcaagccga tacgcgaatc accaatatga gcatagatag cctgattatc
     3781 aataatagca aggacttcca aagtagttcc catgcctctg taagcttcat cctgaccaag
     3841 ctggtgaatc ttttgatttt caatttctag gtaatgggcg aaccattcac gcacttcatt
     3901 gactgtatcg atctgggtat caacccaagc tacacccagg tctgtgaccg ccatttcact
     3961 agcgatattc cctgcacgat gacctcccat cccatcagct aaaataatca tggtacgtcc
     4021 agctctattg acatagtggt tgacatagtc ttggttattt gttcgtttct gaccaacatc
     4081 tgttaataat gaaatttcca tgtgtcagtt ccttcctaat ccgatatctt gcgaaattga
     4141 ctgatgaaga atccatcact tccatacaat tcaggtgtaa tgaggataca gccgtctttc
     4201 atgatatcct tacattcatg ttctagtttt acctgctcga actcgggatg actctctaaa
     4261 aaggccttaa cgacttgaaa attctcctct gagacgatag tgcaggtgct ataagttatt
     4321 ataccacctt tgcctagtat ttgacaaaca ctacctaata tttctaactg aatttcctgc
     4381 aaggacgcga aatctgccgt ttctttattg tatttgatat ctggttttcg gcgcaaaaga
     4441 ccgattcctg aacaaggagc atccaccaaa atcttatcaa aggaatcctg gtcaaaaaac
     4501 tcatgcacct ttctggcatc caatttttga gtttgaaccc gatctgcaac tcccagacgt
     4561 tgggcatttt cttgaattaa atccaacttg tggtcgtaca agtccagagc agtaacctga
     4621 cctgtcgtaa gataagaggc tatatgggct gttttcccac ctggagccgc acaggcatca
     4681 agcactcgct catcaccttg taaatcaagc gtcggagcaa ccagctgact ggactcatct
     4741 tggatggtaa tggctccatt cgcaaacaaa ttatgccctg caaaatgccc ttgctcctta
     4801 accagaccag tggttgctaa aagggaatta ttcgcctcca acaaggcttg gatttcctct
     4861 tttcgactta ggtctgttac acgaatactg gctttgtttc gcactaacag gctttcaaag
     4921 atggcttttg ctctctcctc tccgtattct tccttgagtt tggtaactag ccaaactggg
     4981 agagaatagg caatggagtc acgcttgttt tttcgcttga tgctagcaat atctggccag
     5041 ccttcacgca agatacggcg aaggacagcg ttgaccaatt tttcactgcc ttttttacgg
     5101 agtttggcca attccactgc ttcattgacc acagcatgat ctggaatctt gtccaaatag
     5161 cggagttggt aggcactcat gagaagaagg acatagagcc agctgtctaa ctggtctctg
     5221 tcttcgataa agtgggatag gtaccattcc agagtcaatt tacgggctac cgttccatag
     5281 actagctcgg tcactaagcc cttgtctgct gcagagagct gactcccctt gaggtgttta
     5341 tttaaggcga tatttgaata tgcttggttc acaaaaacat cctctagcac tgctagagct
     5401 aaacttctag ccgtttctac tttagtcacc aaatcgttct cctacagtca atgtacgtcc
     5461 aactccgttg aggaaggaag caatgtccat cttaggctta ccagctggct gcacttgttt
     5521 gagggataga gccccttcag ccgttgcgac aatcaattct ttcttgcaga tagagagaat
     5581 ctcacctgga tttcccttac cttctactgg tagggcttca taaatcttaa agcggtcgcc
     5641 cttaaggaaa gtatgggcaa caggccaggg gttcattcca cgaatttggt taaagagttg
     5701 acgattggtt ttgttccagt ccagtttttc ttcctctggc tttatatttg gagagaaggt
     5761 aacctgactc gtatcctgcg gttcaggttt gatatcacca gcaatatagg caggcagagt
     5821 gtccaaaagc aaatcacgac caactagcgc caatttttca aacaaggcgc caacattgtc
     5881 ctcatctgtg atcggaatgc tgcgacgaga aatcatatct cctgcatcca tttccttaac
     5941 catttccatg atggtcacac cagcttcctc atccccttga atcaaggcat aatggatagg
     6001 cgcaccacca cggtgtctag gaaggaggga ggcatgaacg ttgacagcaa agtccatgct
     6061 atcaaggagt ttgcttggga gaaactgccc aaaagcagca gtcacaattc catctgctcc
     6121 tagcttcata agatcttcca tctctggact tccagataat ttttcaggtt ggtagataga
     6181 tagtcctgct tccttggcag cctgcttgac tggggtttct tggataactt ttttacgacc
     6241 aacagcacgg tctggctggg tcacaacggc tagaatttcg taacggtcat ctgtcaaaag
     6301 tccttttaag actgttgctg aaaagtcggg ggtccccata aagattagtt ttgtcatatc
     6361 ttctcctttt tataaaaatt gctgcggctc atggtcaatg ctgagacgga gctcactatt
     6421 ttcccgttct tgagtcaagg ccaagacctg gttgagggtc ggccccagct catcttctaa
     6481 acggtattta attaaaatct ggtaatgata gaggttgtgg gtacgggcaa tcggttttgg
     6541 cgttggcccc agaatgggac tggtctctga caagcctgac cgcaaaatgt tcatgacttc
     6601 ataggcacgt ttgaaaacct cttcttcttt cttgtgagaa agggtaatac caatcgtgaa
     6661 atagtaaggt ggatagccga gttgtcgtct gattcccatt tcataggcat aaaagccttc
     6721 gtaatcttga tccttggcaa atcgaatagc atagtgctgc gaattgtagg actgtatcaa
     6781 gacttgacct gctttttcag cccgacctgc tcgacctgcc acctgagtca agagctggaa
     6841 ggttctctca gaagaacgga aatcaggcag attcaaggcc gtatccgcat ttagaactcc
     6901 gactagggta acattgggaa aatccaaacc ctttgcaatc atctgagtac caagtaaaat
     6961 atccgctttc cctcgcccaa actgatcaag caaggcttgg tgactgcctt tttttcgagt
     7021 cgtatccaca tccatcctca gaatgcgagc ttggggaaag agttctgcta gctcatcata
     7081 agccttctga gttcccgtcc catagtaacg aatactgcgg ctcttacagt taggacagac
     7141 ctgaggaata tccttcgaga aaccacaata atggcagttc atagtcttgg tatccatatg
     7201 caaggtcaga gaaatatcgc agttgggaca agtatccacc gtcccacact cccgacacat
     7261 gacaaagcta gaataaccac ggcgattgag catgagaacc acctgctctt ttttaaccag
     7321 acggtcttgg atagcctcta gcaaaggagg cgtaaagttt gacgtctcat tttgtccgat
     7381 atagtctcga aagtcaatca cttgaacctc agggattgta gccaaaggat tggcacgttg
     7441 ggttagacgt aagtgttgat agacgccttt gccagcacgt gcccggctct ctaagctcgg
     7501 cgttgcagat ccaagtacca gagttgcttg attatactga gcccgtaaaa tagctacctc
     7561 tctggcatgg taacggggat tgctgtcctg cttataagcc gcttcatgct cttcatcaat
     7621 aatcatgaca cccagatttt tcagaggagc aaagatagca gatctggcac caacaacaac
     7681 ttgggcatcg ccacgctcca ccttgcgcca ttcatcatac ttttcaccat tggataatcc
     7741 tgagtgaaga atggctacct tgtccccaaa acgtgctata aaacgctcgg tcatctgagg
     7801 agtcaaggaa atctcaggta ccagcaaaat agctgtcttg cccttatcca gggcaccttg
     7861 gataatctgc aagtaaacct cggtcttccc acttcctgta atcccttgaa gtagaaaggg
     7921 aggttgagaa ctgccaatag aactcacaac cgcatcacgc acctgtcttt gttctggatt
     7981 taactccaaa ggtctacttg cttcaattcc ttcaaaatag gcagccgagc gttgaacttc
     8041 cttttggact atggtaacag caccttgatc cacaaagaag ttgacttgct ctcgcgagta
     8101 ggactctaac aagctagcca aggaagcgct ctctggatga gacagcagat aatctctcag
     8161 ttccaacttt ttcttggcac gtgtagaaat ctcaacacct tctaattggg catggtcaac
     8221 ctcataccaa gactgggtct tgaccttctt ttgatcgact gcctgatatt ccagaccaag
     8281 caggcctttt ctagtcaaac gcatcatttc agcttgcttg gcaaggtcta gtgaagaaaa
     8341 ggctagcgaa tcttctgaac caaacaggcg tactcgttct tcctgactca agccttccag
     8401 aggatagaga atcttgtcat agctagaatt cagaaatcct ggaagcatgg tcttgaggat
     8461 agagattttg taggagaaga cagatttgcg taactcctca gccagccaga gttgttctgg
     8521 cgtgagaaca ggagaaaaat ccagcacctc tgcaatatct tttaaatctt gctccatctc
     8581 ttctccatct gattgggact ccaaaccaag aacaatccct tgaatcaggc gattaccctt
     8641 accaaaaggc acatgaaccc gcatcccaac ttccagcatt ccctcaaatt cctccggaat
     8701 cctgtaacta tagggctggt ccgtctgcat caagggcaca tctacgataa tcttagctag
     8761 ggccatcttc tcacctcctc cttgtaagta cattcttgca atagaaaaaa taagattgag
     8821 tccccccaac cttaaatttt ttcaccatct tctttttctt tagcaatttg ctctttgatt
     8881 ttcttttctt cttcttcttt gcggcgtttt tcttcttcga tacggcgacg cactgcttca
     8941 cgttttcctt ctggatctgg gtgaattgta acgtttcctg attcgatttc ttctaaagcg
     9001 cgaagagttg atttttcaga cttgaaacct tgagttgctg gggcacctgc ttccaattcg
     9061 tgggcacgtt ttgcttccaa gattacgagt gaatattttg aaggaacctt gtcgagcaag
     9121 gtatcaatag agggttttaa catcatttgc ttgtacctat tttctaaatt ttatcgggta
     9181 gttggagatt ttggtaacat ctcctgatag tgaccaatga cacgatccac acagaagtgt
     9241 tctgcttcaa tcacacattt gacacgttca gcagctaggg gcacctgatc gttgacaatc
     9301 gcataatcat actcacgcat gagggcaatt tcttccttgg ccttttcgat tcgttgggca
     9361 atcacttctg cactatctgt tccacgacct accaagcgat cttgcaattc atccaaatct
     9421 ggtggtgtca ggaagataaa gacagcatct ggaacctttt tcttgacctg aagagcaccc
     9481 tgaacttcaa tttcaaggaa aacatcgatt cccttgtcca aggtttcatt gacataggtc
     9541 agaggagttc catagtagtt gccgacatat tctgcgtatt ccaacatctg tccttgacga
     9601 atcagctctt caaattcttc acgagtacgg aagaaatagt caacaccgtc cacttctcca
     9661 ggacgttgtg cgcgtgtcgt catcgataca gaatattgaa attggttttc agaactctca
     9721 aaaatctctc ttctaaccgt tccttttcca acccctgaag gaccagaaaa aacgattagt
     9781 aagcctcggt ctgccattgt gtctcctttt agtcaatctg tgaaataaca tttctctaga
     9841 ataatggcaa aaagccagat tatcctttac attctttcta tctagtgtaa caaaaaagca
     9901 gtaatttttc aactgctctt tcttatttat ttagcataat ctactgcacg aagctcgcga
     9961 atcacggtta ccttgatatt tcctggataa tcgagattgt tttcaatttt cttacgaact
    10021 ttgtgagcca agattgtgac tttgtcgtcc ttgatttttc ctggattgac catgatacga
    10081 atttcacgtc ctgcttgaag ggcaaagcta gtttgcactc cttcaaagcc gttagcaatt
    10141 tcttccaaat catggagacg cttgatgtag ctttcaagag actcactacg agcacctgga
    10201 cgggctgcgc tcaaggcatc tgctgcagcg acgataactg ctatcacgct ctcagcttca
    10261 acatctccgt ggtgactagc aatcgtattc accacaactg ggggttcctt gtacttacgg
    10321 gccaattcca taccgatttc aacgtggcta ccttcaacct catggtcaat ggctttcccg
    10381 atatcgtgaa ggaatccagc acgacgggca agagccgcat tttcaccaag ttcgctcgcc
    10441 atgataccag ccaacttagc aacctcaatc gaatggcgca aaacattttg tccatatgaa
    10501 gtacggaact gcaaacgtcc cataatcttc atcaagtctg gatgaaggtt tggcgcacca
    10561 atttcatagg cagcagcctc accgtattca cgaatcttat tgtcaatctc ttgacggttt
    10621 ttctcaacca actcttcgat acgagctgga tgtatacgac catctttgag caacatttcc
    10681 atagtcatac gggcaatctc acgacgaatc ggatcaaatc ctgacaaggt caccacttct
    10741 ggtgtatcgt cgataatcac atcgacccct gtcaaacttt caaaggtacg aatgttacga
    10801 ccttcacgac caataatgcg tcccttcata gtatcgtctg gcagatgaac cgttgagttt
    10861 gttgactccg ctacatattc accagcgata cgttgcatag cttgaaccaa gatgtccttg
    10921 gccattttgt cagaacgttc cttgacctct tgctcagctt cgcgaatgcg actggcaatc
    10981 tccctggtca agttttcctc tgtctgagcc aagataatat ctcgtgcttc tgcctgagac
    11041 agagcaccaa tacgctctag ttctgcttct ttttgtcttt cgacttcctc taattgctct
    11101 tcacgcgcat caaggttttt cgctctatca gaaatacttt gttctttttg ttcaagtgtt
    11161 tgttctttac tcgtcaaatt gtcgtcctta cggtcaaggc tagtagctct ctctgtcaaa
    11221 cgactttcga tttgtttgag ttcttgacgt tctgatttga attcagcgtc cacttcttca
    11281 cggtattttc tggcttcttc tttggcctcc aatagtgctt cttttttaag agacttgctt
    11341 tcacgtttgg cttcattaac aagtaaatcc gcttcacgct cagcttgtcc acgtaaatta
    11401 gttgcttctt gttcagcatt taaaagcatc aactctgcag cttcctgaga tgatttcatc
    11461 ttagctgaga tgctgacata tccaatgact aaaccaatga tgacggcaaa aacagcaatc
    11521 gcaagcgaca tgatttccat gtttttacct cattttattg ttattccgaa tgacatacat
    11581 tcttttacat tctaccataa aaaagtgatt ttcacaaacc taaaatagaa tatgttttgg
    11641 ggaaattagt ctgaatttta ggattacact tccttggttt tttaaggaga taagtcagtg
    11701 ccgctatcaa aaagttccac cgaatttcta ccgctatgga ttttctattg ttgtgccaaa
    11761 tagtgttcca ctagattcaa acattttgct ttcaagtctt tgaaaattca ctttaggaaa
    11821 tcacagctta tcttcaagat caaatttg
//
LOCUS       DAVGJL010000059        11638 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.059, whole genome shotgun sequence.
ACCESSION   DAVGJL010000059 DAVGJL010000000
VERSION     DAVGJL010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11638
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.059"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(32..448)
                     /locus_tag="VVE42_001272"
     CDS             complement(32..448)
                     /locus_tag="VVE42_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU3073463.1"
                     /translation="MITIKKQEIVKLEDVLHLYQAVGWTNYTHQPEMLEQALSHSLVI
                     YLALDGDAVVGLIRLVGDGFSSVLVQDLIVLPIYQRQGIGSALMKEALEDYKDAYQVQ
                     LVTEETERTLGFYRSMGFEILSTYNCIGMTWMNRKK"
     gene            complement(460..1155)
                     /locus_tag="VVE42_001273"
     CDS             complement(460..1155)
                     /locus_tag="VVE42_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU3073464.1"
                     /translation="MPVNEYGQMIGESMEGYTPGELPSIDFLEGRYARIEALSVEKHA
                     EDLLAVYGPDTPQEMWTYLFQEPVADMGELVSLLHQMLARKDRFYYAIIDKATGKALG
                     SFSLMRIDQNNRVIEVGAVTFSPELRGTRIGTEAQYLLARYVFEELNYRRYEWKCDAL
                     NLPSRRAAERLSFIYEGTFRQAVVYKGRTRDTDWLSMIDKDWPQVKDRLETWLRPENF
                     DKNGQQYKSLREL"
     gene            complement(1349..3850)
                     /locus_tag="VVE42_001274"
     CDS             complement(1349..3850)
                     /locus_tag="VVE42_001274"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEU3073465.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPS
                     GVGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFTVLAPEHELVDAITSSEQ
                     AEAVADYKHQASLKSDLVRTDLAKEKTGVWTGAYAINPVNGKEMPIWIADYVLASYGT
                     GAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGNVEEAAYTEDGLHVNSDFLDGLNKEDA
                     IAKIVACLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPETELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVATDKVEKRDGSFFHIETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITSKEILAENNGALD
                     KAYNETVKAVTEQIESLKFNTAIAQLMVFVNAANKEDKLYVDYAKGFIQLIAPFAPHL
                     AEELWQTVAETGESISYVAWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDLSREEL
                     QEIALADEKVKAEIDGKEIVKVISVPNKLVNIVVK"
     gene            complement(4305..5393)
                     /locus_tag="VVE42_001275"
     CDS             complement(4305..5393)
                     /locus_tag="VVE42_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEU3073466.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNYPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
     gene            complement(5411..6079)
                     /locus_tag="VVE42_001276"
     CDS             complement(5411..6079)
                     /locus_tag="VVE42_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEU3073467.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHEIRRQAGDDIFIKVPVTPAGLRAIKVLK
                     KEGYHITATAIYTVIQGLLAIEAGADYLAPYYNRMENLNIDSNSVIRQLVLAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVTQN
                     SRSI"
     gene            complement(6098..8536)
                     /locus_tag="VVE42_001277"
     CDS             complement(6098..8536)
                     /locus_tag="VVE42_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEU3073468.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMSIYIEEESMIAGNQASSNKDAPIFPEYTLE
                     FVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKVALDLTDPASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSV
                     WFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGHFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSISKLPTDEIVGGVLLNQKVNPQTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
     gene            complement(8687..9985)
                     /locus_tag="VVE42_001278"
     CDS             complement(8687..9985)
                     /locus_tag="VVE42_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU3073469.1"
                     /translation="MAKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLSSMIVYILAKSLTNGGTFIEMIYSAIQV
                     PLQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASANLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFAAKSKQYQALGKVAAFPAIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGAIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVVTQLLVLVISTVVYFPFFKIQDRLAYQNEIKQS"
     gene            complement(10002..10310)
                     /locus_tag="VVE42_001279"
     CDS             complement(10002..10310)
                     /locus_tag="VVE42_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU3073470.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQASGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
     gene            complement(10356..10676)
                     /locus_tag="VVE42_001280"
     CDS             complement(10356..10676)
                     /locus_tag="VVE42_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU3073471.1"
                     /translation="MEIIVPDQIIMGLILYAGDAKQHIYKALDYIKNGTCERCEEEIQ
                     LADAALLEAHNLQTKFLAQEASGTKTEITALFVHSQDHLMTSMTEINLIKEIISLRKE
                     LHKK"
     gene            complement(10871..>11638)
                     /locus_tag="VVE42_001281"
     CDS             complement(10871..>11638)
                     /locus_tag="VVE42_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HEU3073472.1"
                     /translation="YGLESLEIIPNEFDDNPTILSERISQVAAGVLRNLIDDNMKIGF
                     SWGKSLSNLVDLIHSKSVRNVHFYPLAGGPSHIHAKYHVNTLIYEMSRKFHGECTFMN
                     ATIVQENKLLADGILQSRYFENLKNSWKDLDIAVVGIGDFSNKGKHQWLDMLTEDDFK
                     ELTKVKTVGEICCRFFDSKGKEVYENLQERTIAISLEDLKNIPQSLAVAYGDTKVSSI
                     LSVLRANLVNHLITDKNTILKVLEEDGDLTFREILGE"
BASE COUNT         3600 a         2513 c         1865 g         3660 t
ORIGIN      
        1 ttaaattttg cttaagaaaa aacaagtttt gttatttttt acgattcatc caagtcatcc
       61 ctatacaatt ataggtggat aagatttcaa agcccataga acgatagaag cccaaggttc
      121 tttctgtctc ttctgtcacc agctggactt gataggcatc tttgtaatcc tctaaagcct
      181 ctttcatcaa ggcactacca attccttgac gctgatagat tggtaaaacg attaaatcct
      241 gaaccaatac tgatgagaat ccatctccaa ccaaacgaat caagcccacc acagcatcac
      301 catcaagtgc cagataaatt actaatgaat gagataaggc ctgctccagc atctctggtt
      361 gatgggtata atttgtccaa ccgacagcct gatagagatg taaaacatcc tctagcttga
      421 caatttcttg ctttttaata gtaatcatct caacacctct taaagttctc tcaagctctt
      481 gtactgctgt ccatttttat caaaattttc aggacgcaac catgtttcca aacgatcttt
      541 gacttgaggc cagtccttat caatcataga caaccaatcc gtatctctcg tacgtccctt
      601 ataaaccact gcctgacgga aggttccttc ataaataaag ctcaaacgct ccgcagctcg
      661 tctggatggc agatttaagg catcgcattt ccactcgtag cgacgatagt ttagctcctc
      721 aaaaacatag cgagctagga gatactgagc ttcagttcct atccgtgtac cccttagctc
      781 tggagaaaaa gtgactgctc cgacttctat tactcggtta ttctggtcaa tgcgcatgag
      841 agaaaaagat cccaaagcct taccagtcgc cttgtctatg attgcatagt aaaaacggtc
      901 cttacgagcc aacatctgat gtaaaaggct aaccagctcc cccatatctg ctactggctc
      961 ctgaaaaagg taggtccaca tctcctgagg cgtatcaggc ccataaacag ctagcaaatc
     1021 ctccgcatgc ttttccactg aaagagcctc tatcctagca taacgccctt ctaagaaatc
     1081 aatagaaggt agttcacctg gtgtataacc ttccattgac tccccaatca tttgaccata
     1141 ttcatttact ggcatagctt tctccttttt tcacttttca aactttatag ttgcccatag
     1201 tataccatac tttcataaga agattcaaaa aatcctccag attctactct gaaggattcc
     1261 taccttattt cacaacgata ttaacgagtt tagttggctc aaacagtttg ggaaactgtt
     1321 tgaggttgga aatcagcaaa taaattggtt attttacaac aatattaacc aatttatttg
     1381 gtacactaat cactttcacg atttccttac cgtcaatttc tgctttgact ttttcatccg
     1441 ctagagcaat ttcttgcaat tcttcacgtg ataggtcttt agcgaccatg agtttggcac
     1501 ggactttacc tttgatttgg acaacgattt cgatttcgtc ttcaaccagt ttactttcgt
     1561 cccaagttgg ccaagctaca taagagattg actcacctgt ttctgcgact gtttgccaga
     1621 gttcttctgc caagtgaggt gcaaatggtg caatcaattg aataaagcct ttggcatagt
     1681 caacataaag cttatcttcc ttgttagcag cattgacaaa gaccataagt tgggcaatag
     1741 ctgtgttgaa tttgagagac tcaatttgct cagtaacagc tttgactgtt tcgttgtaag
     1801 ccttgtcaag agcaccattg ttttccgcaa ggatttcttt acttgtaatc aaacggtaaa
     1861 ctcggtcaag gaacttacgg cttccttcca aaccttcttc tgaccaagca atcgaagcat
     1921 cgagtggtcc cataaacatt tcataaacac gaagggtatc ggcaccgtat tgttccacca
     1981 catcatctgg gttaacaacg ttcttgagcg atttagacat cttggctggc gcttgctcca
     2041 actcttcccc tgtttctata tgaaagaagg aaccatcacg tttttcaacc ttgtcggttg
     2101 ccacaagagc accacggtgg tcacggtagc ttgttcccaa aatcatccct tggttaaaga
     2161 gtttttggaa tggttcctta gtcggaacaa caccgaggtc atagaggaat ttatgccaga
     2221 aacgagcata aagcaagtga agtacagcat gttccgcacc acccacgtag atatctactg
     2281 gcaaccattg tttgaggagg tcctcatcag ccaatttctc agtattgtgc gggtcaatat
     2341 agcggaggta gtaccagctt gaaccagccc attgtggcat ggtgttagtt tcacgacgac
     2401 ctttgacacc atcttcacga gtcacttcaa gccaatctgt caagttagct agtggacttt
     2461 caccagtacc tgaaggacgg atatccttgg ttacaggcaa gacaagcggc aattcagttt
     2521 caggaacagc tgttgaagtt ccatcttccc aatgaatgat tggaattggc tcaccccagt
     2581 aacgttgacg gctaaagagc cagtcgcgga ggcggtaggt aaccttctcc tgaccacagc
     2641 ctttttcttc caagcaagcc acaatcttgg caatagcgtc ttctttgttg agtccatcta
     2701 ggaagtctga attgacatgc aggccatcct ctgtgtaggc agcttcttcg acatttccac
     2761 cttcaagtac ttcgacgatt ggaaggtcaa attgtttggc aaattcccag tcacgttggt
     2821 cgtgggcagg cacagccata accgcacctg ttccataact agcaagaaca tagtccgcaa
     2881 tccagattgg catttcctta ccattgacag ggttgatggc ataggcacct gtccaaacac
     2941 cagttttttc cttagcaagg tctgtacgaa ccaagtcaga cttaaggctg gcttggtgtt
     3001 tatagtctgc tacagcttct gcttgctctg aacttgtgat agcgtctact aattcatgtt
     3061 caggagccaa gacagtgaaa gtcgcaccga aaagtgtgtc cggacgagta gtaaagactg
     3121 taaattcctt gtctgttcct tttactttga aagttacatt ggcaccagtt gatttaccaa
     3181 tccagttgcg ttgcatatcc ttgatagact ctgaccaatc tagttcatct aagtcattga
     3241 gcaagcgctc tgcgtaagcc gtgattttga gcatccattg gcgcattggt ttgcggacaa
     3301 ctggatagcc tccacgctca gaagttccgt caggaagcac ttcttcattg gcaatggcag
     3361 ttcccaattc ctcaacccag tttactggca cttcagcttc ataggccaag cctttttcgt
     3421 aaagcttggt gaaaatccat tgagtccact tgtagtagtt tggatctgtt gtgttgactt
     3481 cacgatccca gtcataagaa aatccaagcg cattgatttg acgtttgaag ttggcaatgt
     3541 tttccgctgt aaattctgct gggtcattac cagtatccat agcgtattgc tctgcaggca
     3601 aaccaaaagc atcccaaccc attgggtgaa ggacattgta gccttgcgca cgtttgtaac
     3661 gactgaggat atcggttgca gtataacctt ctgggtgtcc tacgtgcaga ccaactccag
     3721 acggataagg gaacatatca agcgcataaa acttaggttt tgatgcatct gttcctgtct
     3781 taaatgtatg atgttctgcc cagtagccct gccacttagg ctcaatttct ttatgattat
     3841 aaaaactcat ggtctctctc caattttgtg atgttactat tataccattt ttgagggaat
     3901 ttgaaatgtg tttaaagaaa aaactgtata ctgtatctca ttaattttga tgactatgaa
     3961 ttgctctatc tcccactttt tatcccattg ttatgcagta aatcaaggag taagtcaaga
     4021 cgcttatctc gtatagcaca aaatacttat tttagaccga agataaaact ggaatgctaa
     4081 ttaatgtatt cacaataaat agccgacaac ctatgtcgct ttttgattta tgaagaacgt
     4141 atagtagact gaatctgaaa tagtacgaaa caattgctaa aacatttata gaaattaatt
     4201 ttactttccc aatcgatttg ttctcatcct atttcaatct tctatagaat tcaatacggg
     4261 gatcaataaa agactaccat ttggaaaaca gtagtcctta ttgtttattt tgaatttaca
     4321 taggcatcta cagcgataat agcttgagca acatctgaag gcgaaatctt aaacggcatc
     4381 tgatgaattg tctcaccctc catagttgct tgtttaccaa cttttattaa atcatcatat
     4441 ccaacttgat ccaaatgcat ttcttttaga gttgttggca taccaatttt tttgtaaaac
     4501 tcaatatact tatcaagttc ttctttaggt ctattttcca ataatagttg tactaaagtt
     4561 ccataagcta ctttttcacc atgtgttaaa tgatgaatgt cacctgtcaa tgcagtaaaa
     4621 ccattatgaa ttgcatgcgc cgcagctaat cctccacttt caaaacctag accactcaat
     4681 aaagtattag cttcaacaat attttctaat gctggtgtca ccactttagc ttcacaagct
     4741 gccatagcct gtaaaccatc tgcaaacagc gtttcttcac atttcttcgc aattgcaact
     4801 ccagccaatg tttgctgttg tcccaacata gtttttccat ttgcctgcat aaccgcacgc
     4861 gcctcaaccc aagttgctaa accatctgca ataccagacg ctaataaacg cttaggggct
     4921 tgtgaaataa cttttgtatc aaccaaaact aaatctggat ttttagaata aaatagataa
     4981 tgatcaaatg caccttcatc tgtataaata acagataaag cagatacagg tgcgtcggtc
     5041 gatgcaattg ttggagcaat aataacaggc ttttcaatca aatctgcaat agcttttgcg
     5101 ctatcaatcg tctttccccc accaagaccg ataatactat cacaattttc tttctcagcc
     5161 aaggcaacaa ctcgattgat ttcattgtca gaagcttcac cattaaatag cgccagaaca
     5221 atatggaaac catacctatg tagataatct tcaaatcgtt ttccaacaat atcataaacc
     5281 aactgatcgc ataatagaat agggtaattt cccaaatcca aaattgattt ggcattttca
     5341 aacaaggcat tttccccctg aatatatcta gaaggactag caaaaattct catatgtatt
     5401 tcctctctat ctaaatggaa cgactatttt gagtaacaaa ccaatcgtca gaaaaatcat
     5461 caaccgcctt ttggatagat ggcatggcga aagctgattc aaaaacatct gctcctgctg
     5521 taacagcatg cgcacctgca gctaaagcat tatttacttg tgctacattt ttaaaggatg
     5581 cagccaaaat cttactagga gagttctgtc tatcaatagc aagaactaat tgacgaatga
     5641 cagaatttga atcaatgttc agattttcca ttctattata atatggagct aggtaatccg
     5701 ctcctgcttc gatagctaat aatccctgaa taactgtata aatagctgtt gcagtgatat
     5761 ggtagccctc tttttttagc acctttattg cacgtaatcc agctggagta acaggtactt
     5821 tgataaatat atcatctcct gcttgtcttc gaatttcatg agcatccttt aagatgcctt
     5881 caaaatcttg agaaatcacc tgaacatgaa tagagggtgt agagccaatc aattctctta
     5941 catctttgat tcgttcaaaa aaattaatag aaccctctct ttttgcaata gtgggatttg
     6001 aagttacccc agctagcggc aaaatttcag accacttttt aatctcatct aaatttaatg
     6061 tgtcaagcat aaattccata aaaagaacct ctttatttta caaagtatgc tcagtacgtc
     6121 ctataatgtc atcttgggtt gccttagaaa gaacattgaa gaatgcagag tatcctgcaa
     6181 cacgaacaat taagtctctg tgtttttcag gatgtttctg agcgtcaatc aacgtctctc
     6241 tggaaacaac attgtattga atatggtacc catgtaaacg attaaagaat gttcgtaaca
     6301 aagcaattag ttttaattta tcttcttctt tggctaacgt ttgaggattt actttctgat
     6361 ttaagagaac cccacctacg atttcatctg ttggtaattt tgaaatagat tttaaaacag
     6421 atgtagggcc gtgttgatcc atattatgtg atggtgaaca accctctgct aacggtgtac
     6481 ccgcgttgcg tccatctgga gttgctaatg ttccacgtcc ctgccctacg ttggctgata
     6541 tagaagatgt tcctgaataa cgaattcctc caataggccc tcttccataa cgtgtattag
     6601 gatatttagc aatttcatca acataaatgt cataagcagc agtaaccaat ttgtcagcat
     6661 aatcatcatc attaccatac ttaggtgcat catgaatcaa catttcttga atgaccttac
     6721 cttcttctcc ggcataatct gtttccagtg catgccaaag ctgacttggg cttatacgtt
     6781 cttcctcaaa caccaatttt ttaattgcag ctaatgaatc cgacaaattt gcaattccaa
     6841 cttgcaatcc tgatatataa tcatatactg ctccaccttc tttaaggtgt tttccacgac
     6901 caatacaatc atcagtcaat gctgaacata gaatatcagg aacttctcgt tccaatgata
     6961 aatcaataga attttcaaca ataacactca ttcgtgtcaa atatcttagt gttttatccc
     7021 aagcattttc taattcagaa aagttcttca tatccttaaa atgaccaaag cttggtgcaa
     7081 accgtttacc cgaagccgga tcaattccat cattcatcgt gataagtaga accttaggga
     7141 agttcatata actcatacct gtgcaacgat agccccattt ccctggaact gccgtttcaa
     7201 cacatccaat ggcactgtaa tcataagcat catcttccaa tactcctttt gcaataaaag
     7261 aaggaataat aatctcatca ttattaaatg caggcatacc aaaaccaagt ttcatcactt
     7321 caatacactc attcatgaaa cgagcatcta aacctgcatg gtaacgtaca gttagattag
     7381 gttgcggtag atgggtttgt gcaactgatt ttaataccaa ataagataat gggttaacag
     7441 catccttctt atctcgagtc tgtccaccaa ttgtaacatt ttgatataaa ggacttcctg
     7501 ctgaagaaaa tgtatgtgat tgactgcgaa ccttattaat tgtaattgtc ttaatccaaa
     7561 gatttgtcag acgttcaaca atgctatctt ctgtttcttt accactttct aaatcagcct
     7621 tcatatatgg atacatatat tgatcaaaac ggccatatga aagagagtgg ccattagatt
     7681 caatttgtaa aatacattga ataaaccaaa ctgattgaat agcttctgca aaagtagttg
     7741 ccggttcata tgggactcta gagcaaatat ctgcaatctc aagtaattct ttcttacgtt
     7801 taggatttgc attttcggct aaacttttag caagagcaac aaagcgcttt gcatatactt
     7861 taatagcatc gattacgata aatatagagt cgtaaaaatg atatttatca atacttgctg
     7921 gatctgttaa atctagagct acttttgctt tacgagcccg ctcttcaaaa cctcttaaac
     7981 caaattgcaa aagtttctga tagttaactg ctaagtgagc atctccagaa ttcatcttac
     8041 cttccatacc gaagaatcct gtttccatat aaactgacac ttcttcaggt aataaggcac
     8101 cagctctagc acgtaaatta ttattttccc aaaacggagc aatacttcta agttgttctt
     8161 ttgtttcttc tgtaatatag aaaacatctc catcacgctt ttcaaaaaga tccaactcat
     8221 tgagaacaaa ttctagcgta tattccggaa aaataggagc atctttattg gaagaagctt
     8281 gatttcccgc aatcatagat tcttcttcaa tatagataga catattttcc aaaatttctt
     8341 tcagcatata tgcacgtttt aggacattag gttgttcctt gtatcgttca taggcgcgtg
     8401 ttgctagaac tgctctctca gcatcaatat aaggtttttt atttaaaaca tcttctcgat
     8461 atttgttcat cctttcagta aggctgccaa aatattctgt cttgattgtt gttcttgcta
     8521 cttctgtatt taccatcgta agcacctttc tttcgaaaat atttttattt ctttcgaaaa
     8581 tataataccg atttttatag aaattttcaa gcttttaact agaaaaattt taactctcta
     8641 atttttagca caaatgtgtg agtttaacag taacacacaa atacctctaa gattgtttga
     8701 tttcattttg gtaagctaaa cgatcttgta tcttaaagaa tggaaagtaa accactgtag
     8761 atatcaccaa tactaataac tgagtcacaa caccttgcca tccacccacc aaaaatcctg
     8821 atagaatagc tggtgtactc caaggcaatg ttacccctga gaatggctgc atgaaacctg
     8881 ttgcaatagc tccatatact atcacagctg caagtacagg aacaagaatg aaaggtacaa
     8941 acataactgg attcatgaca atcggaaatc caaatacaac tggctcattt acgttaaata
     9001 ttgctggaaa agctgcaact tttcctaagg cttggtattg ttttgatttt gctgcaaaaa
     9061 gcatggcaac tacaagacca aacgtaatcc ctgaacctga tagaattaaa aatgaatcta
     9121 aaaattgttg agtaacaata tgtgcaccat tttctaatga tagattagca gaggctaaca
     9181 tagctttatt agcgtcaaga ttagataaaa gcagagctgt cactactcca tttactaccg
     9241 attgcccatg aacaccaaac caccacaaaa atgatataaa gaatgcaatt ccaatagcac
     9301 catacaaaga tccagttaaa ccttgcaacg gaacttgaat agcagaataa atcatttcta
     9361 tgaatgttcc gccattagtc aatgacttcg ctaaaatata tacaatcata gaagataaga
     9421 aaattacaaa tgctggaatc attgcttcaa actgtttggc aatagcttgt ggaacttgtt
     9481 ctggcatctt aataacaatt tttctcttta taaagaaggt ataaatactt cctactacca
     9541 aacctataat gatagcaccg ataattcctt ggcctccaaa ccaaacttta ctaatagcgt
     9601 ccccaatcgc ctcaccttgt ttagggatat aagatgatct tagcaaaata aagaatgcag
     9661 atacagatag aactccagct ggtaaagcct ctactccgct attcttagca taagaatagg
     9721 caattgaaaa acaagaaatt agacccataa tagcaaaagt tcctgaatat acttgcataa
     9781 acggctctgt ccaattagct ccaaaaacac tagcaatgct cttatttaat ccttcgaacg
     9841 gcaattgtcc cataatcaag aataaactac caactactgt caatggcaaa attgctaaca
     9901 tcccatcttt tagagctata atgccacgca tattcacaaa cttcatcatc ggtgcaatga
     9961 ttttctgaac atccatcttt gccatgataa atctcctttt cttacccact aatcaaagat
    10021 agggccaaat ctaatacttt tttcccatct aacataccat agtccatcat cggaataaca
    10081 gctatcggaa catcacactt atcacaaatt tcttttgatt tatctaatgt ataagcaact
    10141 tgtggaccca atagtgcaac atctatattt ggcgcataat ccgctaattt agactgagaa
    10201 aacgcctcta tttctgcctc aactccacta gcttgcgctg caattttcat attatttaca
    10261 agcataccag tagaaaaacc tgctgcacaa aacaaaccaa tcttcaccat tatgttttcc
    10321 tcctctatgt taataacaat gataatactc tagtattatt ttttatgaag ttcttttctc
    10381 aaactaataa tttctttgat taaattaatc tccgtcatac tagtcatgag atgatcttgt
    10441 gaatgaacaa agagagctgt aatttctgtc tttgtaccag acgcttcctg tgccaagaat
    10501 tttgtttgta gattatgagc ttctaataag gctgcatcag ctaactggat ttcttcttca
    10561 caccgttcac atgtaccatt ttttatgtaa tctaacgctt tataaatatg ttgtttcgca
    10621 tcaccagcat ataaaattaa acccatgata atttgatctg gaacaataat ttccataaat
    10681 tcctcctcat ttcactatgt aaaaagcttt tttcatttga aattttaaaa ttttcttttg
    10741 taactataga atacaactac ataataaagt tgtcaatcca aaaacaacag ctaaactatt
    10801 tttgtttata taagcacaaa tgtgcaatca actaaaaaga aaaacgataa actgaatcag
    10861 tctatcattt tcactcacct agaatctctc taaaagtcaa atccccatct tcttccaaaa
    10921 cttttaaaat tgtatttttg tctgtaatca aatgatttac taaattagca cgcaagacag
    10981 aaagaatcga agatactttc gtatcaccgt aagcaacagc taaactttga ggaatatttt
    11041 ttaaatcttc caaagagatt gctatcgttc tttcctgtaa gttttcatag acttctttac
    11101 cttttgaatc aaaaaatcga caacaaattt ctcctacagt tttaactttg gtcaattctt
    11161 taaagtcatc ctctgtaagc atgtctaacc attgatgttt tcctttattg ctaaaatcac
    11221 caattccgac tacagctata tctaaatctt tccaactatt tttcaaattt tcaaaatatc
    11281 ttgattgcaa aataccatct gctaacaatt tattttcttg cacaatcgtt gcattcataa
    11341 atgtacactc tccatgaaat tttctagaca tttcataaat cagtgtattc acatggtatt
    11401 tagcgtgtat gtgactagga ccacctgcta gaggatagaa gtgaacattt cggacacttt
    11461 tactgtgaat taaatctact aaattactta aacttttccc ccaagaaaag ccaattttca
    11521 tattatcatc aattaaattc ctaaggacac ctgctgcaac ttgagaaatt ctttcagata
    11581 aaattgttgg attatcatca aattcatttg gaataatttc taaactttcc agaccata
//
LOCUS       DAVGJL010000060        11610 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.060, whole genome shotgun sequence.
ACCESSION   DAVGJL010000060 DAVGJL010000000
VERSION     DAVGJL010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11610
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.060"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            24..875
                     /locus_tag="VVE42_001282"
     CDS             24..875
                     /locus_tag="VVE42_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU3073473.1"
                     /translation="MRWDYGQIFKEIRKSKGLTQQDVCGQVIHRTTLTNIEHGKVIPS
                     FENMVFLLEQIDMSLAEFKYICNEYHPSKRRDIIVESQNPSTFQDTRKMVELTEKCQK
                     YLKTHHDVPIQNIYRHTKIVTELRTKGFKNNHVLKDLYEEIWDYLEPMDTWYISDLKL
                     LGTILFFFPSENLPLLIDRIMKIIEKYKYFRETKAFLSSFLANLSTVYFQHHLFKECE
                     TITLQLLVLAEELKIYDILGFSQVRLGILQHNSDLIDKGITLLRLTKEEALVKILEKE
                     INDFSNL"
     gene            complement(1097..1447)
                     /locus_tag="VVE42_001283"
     CDS             complement(1097..1447)
                     /locus_tag="VVE42_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073474.1"
                     /translation="MEHLFKMIILLPCFYFFSWIDKDNRESKFFPIFYYFYWIYITLY
                     ALFSLAWTVFSVLFFNIVLRNLTDIKLWGIWLLLLLIAFASDWLAYVFFKKMIRLRRE
                     LGKSKGVHKGRCRN"
     gene            complement(1587..1922)
                     /locus_tag="VVE42_001284"
     CDS             complement(1587..1922)
                     /locus_tag="VVE42_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073475.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWFYIPFY
                     SLFSLAWTVVSVLFFNTVLRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFRLRQE
                     LGKSKGGRH"
     gene            complement(2013..3323)
                     /gene="der"
                     /locus_tag="VVE42_001285"
     CDS             complement(2013..3323)
                     /gene="der"
                     /locus_tag="VVE42_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEU3073476.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPEMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            complement(3337..4050)
                     /locus_tag="VVE42_001286"
     CDS             complement(3337..4050)
                     /locus_tag="VVE42_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HEU3073477.1"
                     /translation="MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     GLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYSVFGMAL
                     GVPNQHHDMKPRLPLENVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQ
                     FGQAEPSSTRKNLEQKKLL"
     gene            complement(4047..4943)
                     /gene="dnaI"
                     /locus_tag="VVE42_001287"
     CDS             complement(4047..4943)
                     /gene="dnaI"
                     /locus_tag="VVE42_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEU3073478.1"
                     /translation="MESVGDVLKRQPSRFYYQDLVQKIMKDPDVAAFIQQESLTPEEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVKNHGYADVSYEETPELIAAEKEAA
                     IKNRLKLINLPASLKKASLAQVDLDDLGRLPVFEKLLAFVEQYPAIRKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAIGDGNVKTLVDEIKLSEVLILD
                     DIGAEQSTAWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKGKNGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            complement(4944..6113)
                     /locus_tag="VVE42_001288"
     CDS             complement(4944..6113)
                     /locus_tag="VVE42_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HEU3073479.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLALHAPLSSQD
                     FLEHPVYRRLLEKKIGDSAVEDLKVESADGEEIPVSLNQVFPELAELGSQEDLGLKKK
                     VANDFDLDHFRQLMARDGLRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQVI
                     STKRMREKIAQKPVSSDFSPKETTIIKEAKSKTALQFLAEIKQTRKGTTTQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEEAVLRIRER
                     GQKAKTQKQNQTAPVKTNVPKWSNPEYKNETSEETRLELERKKQELLARLEKGGD"
     gene            complement(6114..6587)
                     /gene="nrdR"
                     /locus_tag="VVE42_001289"
     CDS             complement(6114..6587)
                     /gene="nrdR"
                     /locus_tag="VVE42_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEU3073480.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            6727..7092
                     /locus_tag="VVE42_001290"
     CDS             6727..7092
                     /locus_tag="VVE42_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU3073481.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMTH
                     FGYEKEELPGVVSDYIKGV"
     gene            7097..7792
                     /locus_tag="VVE42_001291"
     CDS             7097..7792
                     /locus_tag="VVE42_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3073482.1"
                     /translation="MSLLAFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            7809..8576
                     /locus_tag="VVE42_001292"
     CDS             7809..8576
                     /locus_tag="VVE42_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073483.1"
                     /translation="MFWNLVRYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLAISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAAYIGIQIVIGFIELFFNLSSNFYVNSLVGL
                     NDHFYMGAGIAIVKELIFIAIFYLGTYYILRNKVNLL"
     gene            complement(8929..10128)
                     /locus_tag="VVE42_001293"
     CDS             complement(8929..10128)
                     /locus_tag="VVE42_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU3073484.1"
                     /translation="MRNMWVVIKETYLRHVESWSFFFMVISPFLFLGISVGIGHLQGS
                     SMAKNNKVAVVTTVPSVAEGLKNVNGVNFDYKDEASAKEAIKEEKLKGYLTIDQEDSV
                     LKAVYHGETSLENGIKFEVTGILNELQNQLNRSTASLSQEQEKRLAQTIQFTEKIDEV
                     KENKKFIQTIAAGALGFFLYMILITYAGVTAQEVASEKGTKIMEVVFSSIRASHYFYA
                     RMMALFLVILTHIGIYVVGGLAAVLLFKDLPFLAQSGILVHLGDAISLNTLLFILISL
                     FMYVVLAAFLGSMVSRPEDSGKALSPLMILIMGGFFGVTALGAAGDNLLLKIGSYIPF
                     ISTFFMPFRTINDYAGGAEAWISLAITVIFAVVATGFIGRMYASLVLQTDDLGIWKTF
                     KRALSYK"
     gene            complement(10125..11018)
                     /locus_tag="VVE42_001294"
     CDS             complement(10125..11018)
                     /locus_tag="VVE42_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3073485.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEIGLDGQDIRQETYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLTDKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGRELFPILTQGQYIA
                     TFDQQAPTIDEIFKLESGVEV"
     gene            11135..11215
                     /locus_tag="VVE42_001295"
                     /pseudo
     CDS             11135..11215
                     /locus_tag="VVE42_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
BASE COUNT         3489 a         2501 c         2070 g         3550 t
ORIGIN      
        1 cacatttacc taggaggaac aatatgagat gggattatgg acaaattttt aaagaaattc
       61 gaaagtcaaa aggattgacc caacaagatg tatgtggaca agtcatacat cgaacaactc
      121 taacaaatat tgaacacggt aaagttattc ccagttttga aaacatggta tttcttcttg
      181 aacaaattga tatgagcttg gcagaattca agtatatatg caacgaatac catcctagta
      241 aaaggcgaga tattattgta gagagccaaa atccgtctac ttttcaagat actagaaaaa
      301 tggttgaact cactgagaaa tgtcaaaaat atcttaagac acatcacgat gttcctattc
      361 aaaatatcta tcgtcataca aaaattgtca cagagttacg aactaaagga ttcaaaaaca
      421 accacgtctt gaaagatttg tatgaagaaa tttgggacta tcttgaacct atggatacat
      481 ggtacattag tgatttgaaa ttgcttggaa ccattctctt tttctttcct tctgaaaatc
      541 ttccccttct tattgataga attatgaaaa tcatcgagaa atataaatac ttccgagaaa
      601 caaaagcatt tttatcatct tttttagcca atctctccac tgtatatttt caacatcatt
      661 tattcaaaga atgtgaaaca atcaccctac aacttttggt attagcagaa gaattaaaaa
      721 tatacgatat tcttggattc tctcaagtac gcttgggaat actacaacat aactccgatt
      781 tgattgataa aggaataact ttattacgac taaccaaaga agaggcatta gttaaaatac
      841 tcgaaaaaga aattaatgat ttttcaaacc tataatttgt aataactctc atcattttat
      901 tatagtagat tgtaatagaa tgtgaacgaa tcaattaggg gatctaaagc attttatacc
      961 aatgttttat aagttgtatt gtattattcc atattcaatc cactatacaa gatgaatatt
     1021 tctctcaagt ttcataatca aaataaaaat gactgtatct atagctccat aaacaaatca
     1081 tagatacagt cattttttag ttcctacacc ttcctttatg cacaccttta gatttcccta
     1141 gttcccgcct caagcgaatc atttttttga aaaagacata ggctaaccaa tcactagcaa
     1201 aagcgataag tagtaataat agccaaatac cccataactt gatatctgtc aaatttctca
     1261 agacgatatt gaaaaacaga actgaaaaaa ctgtccaagc aaggctaaaa agagcataga
     1321 gggtgatgta aatccagtaa aaatagtaaa aaattgggaa aaatttactt tctctattat
     1381 ccttgtcaat ccagctgaaa aagtaaaaac agggtaataa gattatcatt ttaaacaagt
     1441 gttccatcac aagcctcctt ttttaaattg ttttctttag tttcgttcta ttaaatgtta
     1501 ttaccaatat aaaccttgga atagcgtgta ataaatgatt ttagaggtac cataagccac
     1561 ctcctacaaa tagaaaccga tataaatcaa tgccttccac ccttagactt ccctagttcc
     1621 tgtctcaagc gaaacatttc tttgaaacag gaataagtta accaattcat accaatagct
     1681 agcagaataa aaagaaacca aatgccccat aacttgatat ctgtcacatt tctcaagacg
     1741 gtattgaaaa acagaactga aacaactgtc caagcaaggc taaaaagaga atagaagggg
     1801 atgtaaaacc agtaaaaata ataaaaaatt ggaaaaaact tactatttct gttggccttt
     1861 tcaatccagt tatcaaaata aaagtacggt gctaaaagta agaatttaaa caaatgttcc
     1921 atcaccgaca tcaccctcct tcttttgata gcgttttcta ttattttatt atatcaaaaa
     1981 aatccggaac tgtcattcca gattctactt ttttatttgc gttttcttgc gatgagatga
     2041 atcggtgttc cctcaaaaac aaaggccttg cggatttgat tttccaagaa acgcaggtaa
     2101 gaaaagtgca tgagttcttc ttcattgaca aagatgacaa aggttggtgg tttggttgcc
     2161 acttgggtcg catagaaaat cttgagacgt tttcctttgt ctgtcggtgt tgggttgatg
     2221 gcaatggcat ccatgatgac atcgttcaag acagctgatg gaatacgtgt attttgactt
     2281 tcgctgattt gcttaatcat ctcaggaagt ttgtggagac gttgcttggt taaagctgat
     2341 acaaagataa tcggtgcgta aggcaggtat tggaactgct cacggatatc ttcttcccag
     2401 tttttcatag tgtggttatc tttttcaagc gtatcccact tgttgaccac gataatcatc
     2461 cctttaccag cttcatgggc aaatcctgcg atacgcttgt cgtactcacg aatgccttct
     2521 tccgcattga tgaccatcaa gaccacatct gaacggtcaa tagcacgcat ggcacgcata
     2581 acagagtatt tctcagtatt ttcataaacc ttaccagact tacgcatacc agccgtatca
     2641 atcatggtaa actcttgacc atctgtatct gtaaagtggg tatcaatggc atcacgagtt
     2701 gttccagcaa caggactagc aataacacgg tcttcgccca agatagcatt gatcaagctt
     2761 gattttccaa cgtttggacg accaatcagg ctaaacttaa tgacatctgg attttcttct
     2821 tcatattcat ttggaaggtt ttccacaatc gcatctagta catcccctgt accgatacca
     2881 tggacagatg agataggcag tggttcaccc aaaccgaggg catagaaatc atagatatca
     2941 tttcgcatct cagggttgtc caccttgtta actgcgagga taactggttt gtgggtctta
     3001 taaagcttac gggctacgta ttcgtctgca tcggtaattc cttccttacc ggacacgaca
     3061 aagacgataa catctgcttc ttccatggca atttctgcct ggtgcttgat ttgttccatg
     3121 aaaggagcat cgacatcatc aattcctcct gtatcaatca tgctaaaaga acgattgagc
     3181 cactcacccg ttgcataaat acggtcacgt gtcactcctt cgacatcttc tacaatggag
     3241 attcgctcac cagcgatccg attaaatagg gttgatttcc caacattggg acgtcctaca
     3301 atggcaatag ttggtagggc cataatttct cactttctac aataatttct tctgttcaag
     3361 attttttcta gttgagcttg gttcagcttg accaaactgt tctgctaggc gctgactcca
     3421 gcttgtggtc gcacgcgccc cagcatagtc agcctgaaca cggtcataag cttggattgc
     3481 ctcagttgac tgttcttggt attcttcctc aaagacaaca ttctctagtg gcagtctcgg
     3541 tttcatatca tgatgttgat ttggcacacc cagtgccatc ccaaagacag aataggtgta
     3601 gtcaggcagg ttaaagagct ctgccacttc ttcagacttg tatcgaacca aaccgataat
     3661 cacaccacca tagcccaagc tttcagctgc caacaaggcg ttttgtccag caagagctgc
     3721 atcgaccgaa ctaatcaaga gaccttccac accttggggt tggaaggtgt cggtatgaag
     3781 tcgggctccc ttttctgctc ggttcaaatc tccgacaaag agaaggaaaa cagcagactg
     3841 gcgaatggct tcttgaggta ccaattcata caaggcatct ttcttctctt gacttcgtac
     3901 cacaatcaca gagtaggatt ggaaattctt ccaagatgat gccatctggg ctgctgtcaa
     3961 aatctcattt aagtctactt ggggaatttc ttgctcttta aacctgcgca ctgaagtatg
     4021 agccttcatc aatttaatgg tttctgtcat cgacggttta ctccttctaa acgagtctcc
     4081 tcagccaaat aacggatgcg ttccatgacc cgtctggctt cccaggtttc gtcatttcca
     4141 tttttccctt tagcgaaatg cttctccaaa tcttcaaagt tgaagttaga ggtgaaaaag
     4201 gtcggtaaat tttcctgcat ccgatattgg agaatgacct gtaggatttc atcacgcacc
     4261 caagcggtgg attgctcggc accaatatca tctaaaatca ggacttcaga aagcttaatc
     4321 tcatccacta aggtcttaac attgccatca ccaatagcat ttttgacatc aatgacaaag
     4381 ctaggatagt ggaggagagt ggatgataca ccacgttttt ctgataaatc atgagctagg
     4441 gcagccacca tgaaactttt acccacacca aagtctccat ataagtaaag accttttcga
     4501 atagctggat attgctccac gaaggctagt agcttttcaa aaactggtaa gcgccccaaa
     4561 tcatccaagt caacttgagc caaactagct ttcttgagac tggctggtag attgattaac
     4621 ttgagacggt tcttaatagc cgcttctttt tcagccgcga ttagctcagg agtttcttca
     4681 tatgaaacat ctgcatagcc atgattctta accaaaatcg gcttgtagcc tttggcaata
     4741 taatccgtat ccccacggag aaacttgtca cgctcggtga tgtactgatt aaacttggag
     4801 atactgcgat ttaattcctc tggagtcaag gattcttgct ggataaaagc cgcaacatca
     4861 gggtccttca tgattttctg gaccaaatct tgataataaa aacggctggg ttgacgtttg
     4921 agtacgtctc cgacactttc catctaatct cctccttttt ctaatcgagc taatagttct
     4981 tgcttcttac gttctagttc cagacgagtt tcctcgctgg tttcattctt atattcagga
     5041 ttactccatt taggaacatt ggtttttact ggggcagtct gattctgttt ttgtgttttt
     5101 gctttctgtc ctcgctcacg gatgcgcaag actgcctctt ctgccgaatg aatcttttga
     5161 taggcatagt cattggctac cttcatggca tatttctcat tgatatttgc cgaatcgacc
     5221 ttattaaagg tcaataaaag aatgatattg atgacttcgt ccagcaagcc caagccagcc
     5281 atctgttgca agagttctct ttctgtttgg gtagtggttc ccttgcgtgt ttgcttgatt
     5341 tctgccaaga actgcagggc agttttactt ttagcttcct tgataatggt tgtttcctta
     5401 ggactaaagt ccgaggaaac tggtttttga gcaatttttt cccgcatgcg tttggttgaa
     5461 ataacctggg aaacagctgt tgacttggcc aattgatagg tctcaaacca agtccacttc
     5521 ttctcctcag caatagcaaa caagttcaag acatcagact gctcatccgc aaaacgaagt
     5581 ccatctcgag ccatcagctg acgaaaatga tccaagtcaa aatcattggc cactttcttc
     5641 ttgagaccaa ggtcttcttg actgcctagt tctgccaatt ctggaaagac ttgattgagt
     5701 gagacaggta tttcttcacc atcagcactt tcaactttca aatcctccac agcagagtcg
     5761 ccaatctttt tctctaagag tctgcgataa acaggatgct ccaagaagtc ttgactagat
     5821 agaggagcat ggagggctag ctgataaaca tccccctttt gatagagggt caagagatta
     5881 aaagcagata agattttcaa tgattttatc agtctatcca tcccaaagtt gagatgattg
     5941 aggatacttg aaaaaagata ttcctttcta ccattatccc aaaaactgat tgtataaaga
     6001 taaaggctca gtgcctcctg accgataatc gggaggtagc actgtaccag agatgaggta
     6061 tcttgcgaca cccgattatt ctttagataa gaaaaacggt caattggctt catttatctt
     6121 tcctttttct ttttagagga ctgggtgatt tgttggagca agctctctaa ctcactgaca
     6181 tccttaaaac tacgatagac actagcaaaa cgtacatagg taatctcgtc caattcagcc
     6241 aactcctcca tgacgagtga accaatgtcc tcactttgaa tttcattttc atttcgacca
     6301 cggagtttct gttcgatacg attgactacc atgttgattt catcacttga cacaggacgt
     6361 ttctgggctg agcggataat cccattaaag attttatctc tggagaattg ttcccgtgtg
     6421 ccatcttttt taacaaccac taaggttctt tcttctactc gttcgtaggt tgtaaaacgg
     6481 tgttggcact cgtcgcactc tcgtcttcta cgaatggtgt tcccttcttc tgcttggcga
     6541 ctatcgataa cacttgactt ggtagcccca cattttggac aacgcatcct ttccctcctt
     6601 atcgttttct tttcattata ccatttttta agcgattccc aaaacaattc ttctttttgc
     6661 ttgacaagtt ttttgttttg ttgtattatt taattaagac aaaagaataa gagaaaggag
     6721 actaagatgt cctggacatt tgacaacaaa aaacccatct atttacagat tatggagaaa
     6781 atcaagcttc agattgtttc ccatacactg gaacccaatc aacaacttcc aaccgtgagg
     6841 gagctagcta gcgaggctgg tgtcaatccc aataccatcc aaagagcctt atcagacctt
     6901 gaacgagaag gatttgtcta cagcaagcga acaactggac gatttgtgac taaggataag
     6961 gagctaatcg ctcagtcacg caaacaatta tcagaagaag aattggaaca cttcgtttcc
     7021 tccatgaccc attttggcta tgaaaaagaa gaactaccag gcgtagtcag tgattatatt
     7081 aaaggagttt aagcctatgt cattactagc atttgaaaat gtatccaaat catatggagc
     7141 aacaccagcc cttgaaaatg tttctcttga cattccagct ggaaaaattg tcggccttct
     7201 tgggccaaac ggctcaggaa aaacaaccct gattaaacta attaatggcc tcttacaacc
     7261 agatcaagga cgtgtcctca tcaacgacat ggacccaagc ccagcaacca aggccgttgt
     7321 agcttatttg cctgatacga cctatctcaa tgagcaaatg aaggtcaaag aagccctaac
     7381 ctacttcaag accttctata aagatttcaa tcttgaacgc gctcatcatc tacttgcaga
     7441 cctgggcatt gatgaaaata gtcgtctcaa gaaactatca aaaggaaaca aagaaaaggt
     7501 tcaactgatt ttggttatga gccgtgatgc tcgtctctat gttttggacg aacccattgg
     7561 tggggtggat ccagcagccc gtgcttatat cctcaatacc attatcaaca actactcacc
     7621 aacttctacc gttttgattt ctacccactt gatttctgat atcgagccaa tcttggatga
     7681 aattgtcttc ctaaaagacg gaaaagtcgt ccgtcaagga aatgtagatg atattcgcta
     7741 cgagtcaggt gaatccattg accaactctt ccgtcaggaa tttaaggcct aagcaaagga
     7801 gattatttat gttttggaat ttagttcgct acgaatttaa aaatgttaac aagtggtatt
     7861 tagccctcta cgcagccgtg ctagtccttt ctgccctcat cggaatacag acacaaggct
     7921 ttaaaaatct accttaccaa gaaagtcagg ctactatgct actttttcta gccacagtct
     7981 ttggtggctt gatgcttaca cttgcgattt caaccatttt cttgattatt aaacgcttca
     8041 aaggtagtgt ctacgaccga caaggctatc tgactttgac cttgccagtt tctgaacacc
     8101 atatcatcac agccaaacta atcggtgcct ttatctggtc attgattagc accgctgtat
     8161 tggctctaag tgctgttatt attctggctt taacagctcc agaatggatt cctctttctt
     8221 atgtgattac atttgtagaa acacatctcc ctcagatctt tcttacaggt atatccttcc
     8281 tactaaatac catttcagga atcctctgca tctacctggc tatttccatt ggacagcttt
     8341 tcaatgaata ccgtacagca ctcgctgttg cagcctacat tggtatccaa atcgtcattg
     8401 gatttattga acttttcttc aatcttagtt ctaatttcta tgtcaattca ctggtaggac
     8461 tcaatgacca tttctatatg ggagcaggta tagccattgt taaagaactc atattcatag
     8521 ctatctttta tctcggaacc tactacatct tgagaaataa ggttaatttg ctttaaataa
     8581 tttttaccta gatatgtaac atactcatag aacaaaagaa accaggcaaa aagtctttaa
     8641 aattagaaaa cgcatagtat caagtgttaa aaatccttga tattatgcgt tttttgtgag
     8701 ctgacttatt tcctttcact atatcgcaaa atgaaataag aacggaacga tgagattttg
     8761 gaattcaaat caatttataa gaatgtttta gaagtaatat tatcctattc cagattcagt
     8821 tcactataca attgagtttt caagcaacct gtttacataa tgtgtacata attaggttcg
     8881 tgattccacc cttttcacct ttaaaaacct cgctttcgca aggctcttct atttataaga
     8941 taaggcacgt ttaaaggttt tccaaatccc taaatcatcc gtttgaagaa cgagactagc
     9001 atacatgcgt ccgataaatc ctgttgctac caccgcaaaa atcactgtaa tagcaagtga
     9061 aatccatgct tctgctcccc ccgcatagtc attaatcgtt cgaaacggca taaagaaggt
     9121 cgaaataaag ggaatataag aaccaatctt caagaggaga ttgtcaccag ctgcacctag
     9181 agctgtcact ccaaaaaaac cacccataat caaaatcatc aaaggcgaca aggctttccc
     9241 tgagtcctca ggacgagaaa ccatagatcc taggaaggct gccaagacta cgtacatgaa
     9301 aagactgatc aaaataaaga gcaaggtatt cagtgagata gcatctccca agtgaaccaa
     9361 aataccagac tgagccaaga atggcaaatc tttaaagagc aaaacggcag ccagaccacc
     9421 tacaacatag atcccaatat gcgttaaaat cactagaaac agagccatca tccgcgcata
     9481 gaaatagtga cttgccctta tgctagaaaa aacgacttcc ataattttgg tgcctttttc
     9541 actggcaact tcctgagctg ttacacccgc ataggtaatc agaatcatat aaagaaagaa
     9601 tcctaaggca cctgctgcaa ttgtttgaat aaacttttta ttttccttga cttcatcaat
     9661 cttttctgtg aattgaattg tctgcgctaa gcgtttttcc tgctcttgag acaaggaagc
     9721 agttgaacga ttaagctgat tttgcagttc attgagtata cctgtaacct caaatttaat
     9781 tccattttca agcgatgttt cgccatgata aactgccttt agaacactat cttcttgatc
     9841 aatggtcaaa taacctttta atttttcttc tttaattgct tctttggcac ttgcttcatc
     9901 tttatagtcg aagttaacac catttacatt cttcagtcct tctgctacag atggcactgt
     9961 tgtcactact gccactttat tatttttagc catagaagaa ccttggagat gcccaattcc
    10021 tacagagatt cctaaaaaga ggaacggcga aatcaccata aagaagaaac tccatgactc
    10081 gacatgtcga agataggttt ccttgattac aacccacata tttctcatac ttccactcct
    10141 gattctagtt taaagatttc atcgatagtt ggcgcttgtt ggtcaaatgt tgcgatatat
    10201 tgaccttgag tcaagattgg gaagagttcc cttccagcgc tctcatcctc caaaatcaat
    10261 ttccaactgc cttgtttggt caagctcacc tgtttgacat gaggaagatt ttccaattct
    10321 tccttgcttc gttcacttga aacaaagaga cgcgttttcc cgtattgatt gcggacatcc
    10381 tgaactggtc cgtgcaagac cacacggcca tctcggatca tcagaatatc gtcacaaagt
    10441 tcctcaacat tggtcatgac atggtcagaa aagataatgg ttgctccgcg ctctttttcc
    10501 tgaaaaatga cttgtttgag caattctgta ttaactgggt ccaatccact aaaaggctca
    10561 tccaagataa tcaggtctgg ttcatgaatc agagtaataa tgagctgaat cttctgctga
    10621 tttccttttg acagactctt gattttatct gtcagctttc ctttcacttc caacctcttc
    10681 atccattgag ggagtttttc tttgacttct ttggcatcca tgccttttag agtcgccaag
    10741 tagcgaactt gttcaagaac tgtcaattta ggcatgagac tgcgttcttc aggcagataa
    10801 ccaatccgag cataggtctc ctgacgaata tcctgaccat ccagaccgat ttctccctga
    10861 tattctagga atttcaaaat actatggaaa atcgttgttt ttccagcacc attttttccg
    10921 actagtccca aaatacgacc tggtcgcgct tgaaagtcaa taccaaacaa aacttgcttg
    10981 gatccaaaac ttttctctag acttcttact tctagcatct ttcacctccg aaatttcttg
    11041 cactcattat actccttttt gatagccttt acaatgtttt ttgtccattt ttagaagact
    11101 attgctgtgt aaaatatggc ctggagcact tttatactca atgaaaagca aagagcaaac
    11161 tagaaagcta gccgtaggct gctcaaagta cagctttgag gttgcagata aaactgacga
    11221 agtcagctca aaacactgtt ttgaggttgt ggatagaact gacgaagtca gtaactatat
    11281 ctacggcaag gcgaagctga cgtggtttga agagattttc gaagagtatt agtgataaat
    11341 ccattataca gtagcaaact taatttatac cttccgctcc tcaactgtct atttttaatc
    11401 ctgaattgtt atttgagtaa ctcctttttc ctcataaagt tttcttcctc taaaacttct
    11461 ggaaaaaggc gaatagtttc agacaacatt tttataagaa acaagttcat ctgtcatttc
    11521 aagaaggagt aatcctttat ctactaatgg acggaacaga attcaaccgc ttgtcccata
    11581 tgttttctaa ggattatata gtaaaatgaa
//
LOCUS       DAVGJL010000061        11534 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.061, whole genome shotgun sequence.
ACCESSION   DAVGJL010000061 DAVGJL010000000
VERSION     DAVGJL010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11534
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.061"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            75..1586
                     /locus_tag="VVE42_001296"
     CDS             75..1586
                     /locus_tag="VVE42_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEU3073486.1"
                     /translation="MSEKAKKGFKMPSSYTVLLIIIAIMAVLTWFIPAGAFIEGIYEA
                     QPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESTLSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNAWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     VIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALALGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
     gene            1608..2939
                     /locus_tag="VVE42_001297"
     CDS             1608..2939
                     /locus_tag="VVE42_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEU3073487.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQDVL
                     EKTLEICRDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQFKKRVRFIFGTDEETLWRCMARY
                     NTIEEQASMGFAPDSSFPLTYAEKGLLQVKLHGPGSDQLELEVGGAFNVVPDKANYQG
                     LLYEQVCNGLKEAGYDYQTTEQTVTVLGVPKHAKDASQGINAVIRLATILAPLQEHPA
                     LSFLATQAGQDGTGRQIFGDIADEPSGHLSFNVAGLMINHERSEIRIDIRTPVLADKE
                     ELVELLTRCAQNYQLRYEEFDYLAPLYVAKDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPGAKQTEHQANECAVLEDLYRAMDIYAEAVYRLAT"
     gene            3034..3161
                     /locus_tag="VVE42_001298"
                     /pseudo
     CDS             3034..3161
                     /locus_tag="VVE42_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001836165.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fucose isomerase"
     gene            complement(3344..3631)
                     /locus_tag="VVE42_001299"
                     /pseudo
     CDS             complement(3344..3631)
                     /locus_tag="VVE42_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3619..3870)
                     /locus_tag="VVE42_001300"
     CDS             complement(3619..3870)
                     /locus_tag="VVE42_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657761.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEU3073488.1"
                     /translation="MKEFQFERKQRFSLRPYAIGACSVLLGTSLFFAGMGAQPVQDTE
                     TSSALISSHYLDEQDLSEKLKSELQWFELENKLLNLWEH"
     gene            4074..4151
                     /locus_tag="VVE42_001301"
                     /pseudo
     CDS             4074..4151
                     /locus_tag="VVE42_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(4315..5199)
                     /locus_tag="VVE42_001302"
     CDS             complement(4315..5199)
                     /locus_tag="VVE42_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU3073489.1"
                     /translation="MIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     IRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
     gene            complement(5344..6495)
                     /locus_tag="VVE42_001303"
     CDS             complement(5344..6495)
                     /locus_tag="VVE42_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEU3073490.1"
                     /translation="MATFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKYIEGSD
                     ILPKVLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHGCDFIISLGGGSPQDAA
                     SCISIMAINGGKPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMLSKPKGLTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVENGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIAEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            complement(6641..8407)
                     /locus_tag="VVE42_001304"
     CDS             complement(6641..8407)
                     /locus_tag="VVE42_001304"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HEU3073491.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLK
                     YPDGEPVECVISPSTIGRVPEAAASHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAIWGFNGTERPGAVYLAAVLASHAQKGIPAFGIYGRDVQEANDTAIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRG
                     IYDPEEFERALKWVKENVKEGFDHNREDLVLSREEKDRQWEFVIKMFMIGRDLMVGNP
                     RLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVKRVTGHTLEGCAAAGFLHLINSG
                     SCTLDGTGQATRDGKPVMKPFWELEESEVQAMLENTDFPPANREYFRGGGFSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDPGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEDLESADYRACQLLGPLHK"
     gene            complement(8464..11523)
                     /locus_tag="VVE42_001305"
     CDS             complement(8464..11523)
                     /locus_tag="VVE42_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEU3073492.1"
                     /translation="MLCFGLLAGVKADNRVQMRTTINNESPLLLSPLYGNDNGNGLWW
                     GNTLKGAWEAIPEDVKPYAAIELHPAKVCKPTSCIPRDTKELREWYVKMLEEAQSLNI
                     PVFLVIMSAGERNTVPPEWLDEQFQKYSVLKGVLNIENYWIYNNQLAPHSAKYLEVCA
                     KYGAHFIWHDHEKWFWETIMNDPTFFEASQKYHKNLVLATKNTPIRDDAGTDSIVSGF
                     WLSGLCDNWGSSTDTWKWWEKHYTNTFETGRARDMRSYASEPESMIAMEMMNVYTGGG
                     TVYNFECAAYTFMTNDVPTPAFTKGIIPFFRHAIQNPAPSKEEVVNRTKAVFWNGEGR
                     ISSLNGFYQGLYSNDETMPLYNNGRYHILPVIHEKIDKEKISSIFPNAKILTKNSEEL
                     SSKVNYLNSLYPKLYEGDGYAQRVGNSWYIYNSNANINKNQQVMLPMYTNNTKSLSLD
                     LTPHTYAVVKENPNNLHILLNNYRTDKTAMWALSGNFDASKSWKKEELELANWISKNY
                     SINPVDNDFRTTTLTLKGHTGHKPQINISGDKNHYTYTENWDENTHVYTITVNHNGMV
                     EMSINTEGTGPVPFPTPDKFNDGNLNIAYAKPTTQSSVDYNGDPNRAVDGNRNGNFNS
                     GSVTHTRADNPSWWEVDLKKMDKVGLVKIYNRTDAETQRLSNFDVILYDNNRNEVAKK
                     HVNNLSGESVSLDFKEKGARYIKVKLLTSGVPLSLAEVEVFRESDGKQSEEDIDKITE
                     DKVVSTNKVATQSSTNYEGVAALAVDGNKDGDYGHHSVTHTKADSNAWWQVDLGEEFT
                     VSKVDIYNRTDAEPQRLSNFDVIFVSSSGEEVFRRHFDKVVDGLLSLKVPSVGAKLVK
                     IELKSAAIPLSLAEVEVYGSKRTPKKLSNIALTKETRQSSTDYNGFSRLAVDGNKNGD
                     YGHHSVTHTKGDSPSWWEIDLAQTEELEKLIIYNRTDAEIQRLSNFDIIIYDSNNHEV
                     FKQHIDSLESNNLSIDLKGLKGKKVRISLRNAGIPLSLAEVEVYTYK"
BASE COUNT         3309 a         2468 c         2038 g         3719 t
ORIGIN      
        1 agcgttttct taaatgttta tttaagaaag tagttggttt tttacacttt gttagacatc
       61 aggaggaaaa acaaatgagt gaaaaagcta aaaaagggtt taagatgcct tcatcttaca
      121 ccgtattatt gataatcatt gctattatgg cagtgctaac ttggtttatc cctgcggggg
      181 cctttataga aggtatttac gaggctcagc ctcaaaatcc acaagggatt tgggatgtcc
      241 tcatggcacc gattcgggct atgctaggta ctcatccaga ggaaggttcg ctcattaaag
      301 aaacgagcgc agcgattgat gtagccttct tcatccttat ggttggtggt ttccttggca
      361 ttgtcaacaa aactggtgct cttgacgtag ggattgcctc tatcgtgaag aagtataagg
      421 gccgcgaaaa aatgttaatt ttggtactga tgcctttgtt tgccctcggt ggtacaactt
      481 atggtatggg ggaagaaaca atggccttct atccactcct tgtgccagtt atgatggccg
      541 ttggttttga tagcctgact ggtgttgcaa ttattttgct cggttctcaa atcggctgtt
      601 tggcatctac tctgaatcca tttgcgacag gtattgcttc agcgactgcg ggagttggta
      661 caggggacgg tatcgtactt cgtctgatct tctgggttac cttgactgct cttagtactt
      721 ggtttgttta ccgttatgcg gataagattc aaaaagatcc gactaagtca ctggtttata
      781 gtactcgcaa agaagatttg aaacacttta acgtagaaga atcttcatct gtagaatcta
      841 cacttagcag caaacaaaaa tcagttctct tcttatttgt gttgacattc atcttgatgg
      901 tattgagctt cattccatgg acagaccttg gcgttaccat ttttgatgac tttaatgctt
      961 ggttgactgg tcttccagtt attggtaata ttgtcggttc atctacttct gcactaggta
     1021 cttggtactt cccagaaggc gccatgctct ttgcctttat gggtatcctg attggtgtta
     1081 tttatggtct taaagaagat aagattatct cttccttcat gaatggtgct gctgacttgc
     1141 tcagtgttgc cttgatcgta gcgattgctc gtggtattca agttatcatg aacgacggta
     1201 tgattaccga tacaatcctc aactggggta aagaaggctt gagcggtcta tcttcacaag
     1261 tctttatcgt tgtaacttat atcttctatc tacctatgtc attcttgatc ccatcttcat
     1321 ctggtcttgc cagcgcaact atgggtatca tggctccact tggagaattt gtaaatgtcc
     1381 gtcctagctt gattatcact gcttaccaat ctgcttcagg tgtcttgaac ttgattgcac
     1441 caacatctgg tattgtgatg ggagctcttg cacttggacg tatcaacatt ggtacttggt
     1501 ggaaattcat gggcaaactc gtagtcgcta ttattgtagt gaccatcgcc cttcttctcc
     1561 ttggaacctt ccttccattc ctataaaata gtgagtgagg tgattccatg aaaatagata
     1621 taacaaatca agttaaagat gaatttctta tatcattaaa aaccttgatt tcctatcctt
     1681 cagtactcaa tgaaggagaa aatggaacac cttttggaca agcaatccaa gatgtcctag
     1741 aaaaaacttt agagatttgt cgagacatag gtttcactac ctatcttgac cctaaaggtt
     1801 attacggata tgcagaaatc ggtcagggag cagagcttct ggccattctc tgtcatttgg
     1861 atgttgttcc atcaggtgat gaagcagatt ggcagacacc gccatttgaa gcaactatca
     1921 aagacggctg ggtattcgga cgtggtgtcc aagatgataa aggcccttcg ctcgcagctc
     1981 tctatgcagt aaaaagcttg ctggaccaag gtattcagtt caaaaagcgc gtacgcttta
     2041 tctttggtac cgatgaggaa accctctggc gctgcatggc acgctacaat accatcgaag
     2101 aacaggccag tatgggcttt gcacctgact catcttttcc tctgacctat gctgaaaaag
     2161 ggcttctaca ggtcaaactt catggcccgg gatcggatca actagagctt gaagtaggag
     2221 gcgcctttaa cgttgtacca gacaaggcca actaccaagg tctcctctat gaacaggttt
     2281 gtaacggtct caaagaagct ggttatgatt accaaaccac tgaacaaacc gtaacggttc
     2341 tcggagtgcc aaagcatgct aaggatgcta gtcaaggtat caatgctgtc atccgactag
     2401 ctaccattct tgctcctctc caagaacacc ctgctctcag ctttcttgca acacaagcag
     2461 gtcaagacgg cacaggaaga caaatctttg gtgatatagc agatgaacct tctggtcacc
     2521 tatcctttaa tgtcgcaggt ctcatgatca atcatgaacg ttctgaaatc cgtattgaca
     2581 ttcggactcc tgtcttagct gataaggaag aactagtaga gttgcttaca agatgtgcac
     2641 aaaactacca actccgctac gaagagtttg actatctagc gcctctatac gtcgcaaaag
     2701 acagtaaact cgttagcaca ctgatgcaaa tctaccaaga aaagactggc gataacagtc
     2761 ctgctatttc atccggtggt gccacttttg ctcgcaccat gccaaattgt gtagccttcg
     2821 gcgccttatt cccaggagcg aagcaaacag aacatcaggc aaatgaatgt gccgttctag
     2881 aagatttgta ccgtgctatg gatatttatg ccgaagccgt ctatcgactt gcaacttaat
     2941 caggcaactg tttctaccaa aaaaaatcga ccgattaatg aactgcaccc caaaagttag
     3001 acagaataaa tctaactttt ggggtgtttt attatgaaat tgaattatga agataaagtt
     3061 cagatctatg aactaagaaa gcaaggacaa agcttcaaac agctttcaaa aagatttgtg
     3121 tggatgtttc tggtctaaag tcatctgaat ctttgagatg agctttataa atcgcttttt
     3181 tcagtttttg cactggtgtt tcgataaact caaacttttt agccgtggta ttgcctgatt
     3241 ttatagtata ttgaaactag aatagtacac ctctacttct aaaacatttt tagaaatcga
     3301 tttgactgtc ctgaacgatt tgtcctgtta ttatttcatt ttactatatt tgagccactt
     3361 cgtctttaac ggctttattc ataagctctt gtaatttttc tttactatca attacttctg
     3421 attttccgtt gtaatttatt gtaataggtt ttaacttacc taatttctcg acacgctcat
     3481 taatttgatc ttttttgaag gctgcatatg tttttcctaa gattttttcg aaaatatatt
     3541 tatcagatag cggtttgtct tcttcagctt ggtttttgta ttaatttgaa acgtaaggaa
     3601 caaatccttc atagtaacct aatgctccca taagttcaaa agcttgtttt ctaattcaaa
     3661 ccattgcaac tcagatttca gcttttcaga taaatcctgc tcatccaaat aatgacttga
     3721 aattagtgct gaactcgttt ctgtatcctg tacaggctga gcacccatac cagcaaaaaa
     3781 taaactcgtt cctagcaaga ccgaacaagc tcctattgca tatggcctca aagaaaaacg
     3841 ctgctttctc tcaaattgaa attctttcat cccatctccc atcattcatt attactgtat
     3901 attttgtata tcagaaatag tttgtattca caaatctttc tagttattcc cttatcattc
     3961 ctaattaagg gagataacat acaataattt ttagttaaat gtatatcgat gttttttgtt
     4021 tttcttaata aacgcaatac aaaaagagcc tgttgccaag ctctttgtac tcaatgaaaa
     4081 tcaaagagca aactaggaaa ctagccacag gttgctcaaa acaccgtttt gaggtagtag
     4141 ataagactga cgaagtcagc tcaaaacact gttttgaggt tgtggataga actgacgaag
     4201 tcagctcaaa acactgtttt gaggttgcag atagaactga cgaagtcagt aacatctata
     4261 cggcaaggcg acgctgacgt ggtttgaaga gatttttcga agagtattag tctattattt
     4321 cttctcagcg cgaagggctg acaagatttg tgttcggata tcatccacac catttggcgt
     4381 atttggtaaa aagatggttt gatttccttt agaggcaaag gtattcaagg tatccaaata
     4441 ctggttggtc aagaggatag acatgatttg ttcttctgtc atgccaacat tggcttcctt
     4501 gagttcggtg atagactctg ccaatccatc cacaatcgcc ttacgttgtt gggcaatccc
     4561 cacaccatga aggcggtctt tttctgcttc tgcttcagct gcagtgacaa ttttaatctt
     4621 gtcagcttcc gccaattctt gtgctgcgac ccgcttacgt tgcgccgcat tgatttcatt
     4681 catggattgc ttaacttctg catccggttc gaccttggta atcaaggttt tcacgataat
     4741 gtagccgtaa gtggtcattt cttctgctac ttggtgttga acctcaaggg caatctcatc
     4801 ttttttctca aacaattcat ccaaggttaa ttttggaaca gaagagcgaa gagcgtcttc
     4861 gatataagat ttaatctgag attctggacg tatgagttta tagtaggcat ctgtcacgct
     4921 ctgctcgttg acacggtact gagtcgctac attcatcata acgaacacat tgtccttggt
     4981 cttagtctca accacaatat cactttgcaa caagcgcaac tgaatccgtg ctgcaatcga
     5041 gtcaatccca aaaggcaagc gaatatgaat accgctatta gcaacctttt ggtatttccc
     5101 aaagcgttca ataatcgcca ccgactgctg acgaaccaca taaactgtac tcagtgtgac
     5161 tatcaccaat aggagcacac aaacaatcag aaaaatcatg aaaaatattg ccataatgga
     5221 acctccacaa gtatttttct agtattatag cacatttaaa gaaggctgtg ccgtttttac
     5281 tgcgattttt cctgaaatgt caataattag aggtgaattg tcctattgtc gtccaatctc
     5341 ttgctaaaat aactctttat aaaaggcaat cgtttcttct aaggttggca taaatggatt
     5401 tcctggtgcg caggcatcaa tcaaggcatt cttagaaagg tattcaaagt cgaaatcttt
     5461 ttcttcaata ccaagttcag tcagtttctt aggaatacct actgtctcag aaagcttctc
     5521 aatctcagca atcgcataat cggcacattc ttgatctgat ttaccttcta catgaagtcc
     5581 caaggctttg gcaacattgc ggaaagcttc tggtacacgt ttagcatttt cacgttctat
     5641 aactggtagc aacatggcac agcacacgcc atgtggcaaa ttatatactg caccgagttg
     5701 gtgagccatt gagtgaacat acccaagacc agcattatta aagctcatac caccaaggaa
     5761 gatggcattg accatacctt cacgcgcttc aatatcatgt ccattttcta cagcacgagg
     5821 aagatattcc ttgataagct caatagcacc aatagacagt ttcttggtta catcataagc
     5881 accaggtgtt accaaagctt caacagcgtg agtcagagca tccataccag tagcagcagt
     5941 caggccttta ggtttggaaa gcatgagttc cgggtcatta acagagataa gagcaaggct
     6001 attcttgtca accattacca tcttaacctt gcgttcttca tcagtaatca catagttaat
     6061 agtgatttct gcagaagttc ctgctgttgt attgatagcc acaactggca agcctttttt
     6121 agcagactta tgaagccctt cataatcctg tggttttcca ccatttatag ccatgataga
     6181 aatacaacta gctgcatcct gtggagaacc cccgccaaga ctgatgataa agtcacagcc
     6241 atgttcttgc aaagcagcta ccccatctgt gacattttta caagtagggt ttggctctac
     6301 atcgctaaag atgacatatt cgattccttc tgtatccagt ggttttaaaa ccttaggcaa
     6361 aatatcactg ccttcgatgt acttatctgt aaccaaaagt gcctttttat agccaagttc
     6421 cttgatataa ggacccactt catttacaac acctttacca ataaggttga ctgccggaac
     6481 ataaaatgta gccatataat tttcctcctg cgcctccgcg ctatctattt aacatcatta
     6541 tattactttt tgtattcgtt ttcaattatt tgtgaattgt ttaacaaatc tttttaaaag
     6601 gataggaggg acgtaagttc acctcctata taaacaagtt ttatttatgt agtggtccca
     6661 acaactgaca tgcacgatag tctgctgatt ctagatcttc tgttccaaat aaggaccaat
     6721 ttttaggtct aaagatatct tcctcaggta cattatgcat attgacagga attctcaaca
     6781 tagaagccaa ggtaatcaag tctgctccaa tgtgtccata tgttatggct ccgtgattag
     6841 ctccccaatt attcatgacg tcatagacag acttgaaagc accttttcct gtcaaacgtg
     6901 gagcaaacca agtagttggc catcctggat ctgtacgatt atctaaagta tggtgaacat
     6961 cttcaggaag ttcaagtgta taaccttctg caatttgtag cactggacca acccctttta
     7021 gaagattgag acgtaccatt gttactggca tatccccctt cgtcaagaaa cgagttgaga
     7081 atcctcctcc acggaagtat tcgcggtttg ctggtgggaa gtctgtattt tcaagcatag
     7141 cctgcacttc actttcttcc aactcccaga atggtttcat aacaggtttg ccatctcgag
     7201 tagcttgacc tgtaccatcc aatgtacaag aaccagagtt gattagatgt aagaagccag
     7261 ctgcagcaca accctctaaa gtatgtcccg ttacacgttt aacagcctct ggactccaat
     7321 aagtgcgcac atcagcaaag atttgtggag tatttgttaa tagataatta aagagcatag
     7381 acacaccatt tagtgaatca ttctctgtcg caaatacaaa tggttttcga ataccattcc
     7441 agtcaaactg agtattgagg aaagtttcca taaagtcccc atttgggaaa tgatctgtcc
     7501 actggcgttg accttggaaa ccagctacta aagcatggtg accaactgct tcttcttcaa
     7561 aaccaagttc agcaagtctt gggttaccaa ccattaagtc acgtccaatc atgaacatct
     7621 taataacaaa ttcccattgt ctatcttttt cttcacggct taaaacaagg tcttcacggt
     7681 tatggtcgaa tccttctttt acgttttctt tcacccattt gagcgcacgt tcgaactctt
     7741 cagggtcgta aataccacgg tccatacggc gtgtgaactc cgtcatatct accgattcat
     7801 ttcgcattcc taagtattct tggaagaaat ctggatttac aatagaacca ccaatcccca
     7861 tcgaaacact acccattgat aggtaagcag tgtctctcat caagccagtt gcaagagctg
     7921 cacgcgcata acgtaaaagt ttttctttga catcttctgg aatagctgta tcattagctt
     7981 cctgaacatc tctaccataa atcccaaagg ctggaatccc tttttgagca tgtgaagcta
     8041 gtacagctgc aagatagaca gctcctgggc gttctgtccc attaaatccc caaatagcat
     8101 gaggaatatc tggagacata tccatagttt cactaccata acaccagcat ggtgtaactg
     8161 taattgttgc gcaaacattt gattttttaa acaactcatg ggaagctgca gcctctggaa
     8221 cacgaccaat ggtagatgga gaaatcacac attccacagg ttccccatct ggatatttca
     8281 atgtgcttga aatcaaatct gccacacttt tagccatgtt cattgtttgt acttcaagtg
     8341 attcgcgtac accttgacga cgaccatcaa tagtcggacg aatcccaata cgtggatgtt
     8401 gaatcatact attttcctct tatttttctg tttctttcat actaccgatg ataattttta
     8461 aaattactta taagtataaa cctctacctc tgccaaactt aaaggaattc ctgcgtttct
     8521 caaagaaatt ctaacctttt ttcccttcag cccttttaag tctatggata gattattgct
     8581 ttctaaactg tcaatatgtt gtttaaaaac ttcatgatta tttgaatcat atataataat
     8641 atcaaaattt gataatctct gaatttcagc atctgttcta ttataaataa ttaacttttc
     8701 taattcttcg gtttgtgcta aatctatctc ccaccatgaa ggagaatctc ctttggtatg
     8761 agtcactgaa tgatgaccat aatctccgtt tttatttcca tcaactgcta gacgagaaaa
     8821 accattgtaa tccgttgaac tctgtcgagt ttctttggtt aatgcaatat tagaaagttt
     8881 cttcggagtt ctctttgaac catagacttc aacttccgct aaacttaacg gaatagctgc
     8941 tgattttaat tctattttga ctagcttagc ccctacagaa ggtactttta aagataacaa
     9001 accatcaact actttatcaa aatgtcttct aaaaacttct tctcctgatg aagatacaaa
     9061 aataacatca aaattagata aacgctgagg ttcggcatct gttctattat aaatatcaac
     9121 tttagaaacc gtaaactctt ctcccagatc gacctgccac caagcgttag aatctgcctt
     9181 agtatgagtc accgaatgat gtccgtaatc tccatcttta ttaccatcaa ctgctaaagc
     9241 agctacaccc tcataattgg ttgaactttg agtagctacc ttatttgtag agactacttt
     9301 atcttctgtt attttatcta tatcctcttc agattgctta ccatctgatt ctctaaaaac
     9361 ctctacttct gctaaactca aaggcactcc actcgttagt aatttaactt taatatacct
     9421 tgctcctttt tctttgaaat ctagactaac agattcaccc gacaaattat taacatgttt
     9481 cttagcaact tcgtttctat tattgtcata tagaatcaca tcaaaattag atagacgttg
     9541 agtctcagca tctgtgcgat tataaatttt aacaagccca actttatcca tttttttcaa
     9601 atcgacttcc caccaagagg gattatctgc cctagtgtgt gttaccgaac cagagttaaa
     9661 attaccattt ctgttaccat ccacagctct attagggtct ccattgtaat ctacagaact
     9721 ttgtgttgtt ggttttgcat atgctatatt caaattacca tcattaaatt tatctggtgt
     9781 tgggaaaggg actgggcctg tcccttcagt atttatagac atctctacca ttccattatg
     9841 attaaccgta atggtataaa catgggtatt ctcatcccaa ttttctgtat aagtataatg
     9901 atttttatcg ccacttatat ttatctgagg tttatgacca gtatgccctt ttaatgtaag
     9961 tgttgttgtc ctaaagtcat tatctacagg attgatggaa taatttttgc ttatccagtt
    10021 cgctaactct aattcttctt tcttccaact ttttgatgca tcaaaatttc ctgataatgc
    10081 ccacatagct gtcttatctg tcctgtaatt gttcaataaa atatgtaaat tatttggatt
    10141 ttctttaaca acagcgtaag tatgtggcgt caaatctaac gataacgact ttgtattatt
    10201 agtatacata ggcaacatta cttgctgatt tttattgata ttagcattac tattataaat
    10261 ataccaggaa ttacctacac gctgagcata cccatctcct tcataaagtt ttggataaag
    10321 cgagtttaaa tagttgactt tactagacaa ttcctcacta tttttagtca aaatttttgc
    10381 attagggaat atagatgaaa tcttttcctt atcaattttc tcatgtatta caggaagaat
    10441 atgatatctc ccattattat ataaaggcat tgtttcatca ttcgaataaa gtccttgata
    10501 aaatccgttt aatgaactaa tcctaccttc tccattccaa aatacagctt ttgttctatt
    10561 tacaacttct tccttacttg gagctggatt ttgtatagca tgtctaaaga aaggaataat
    10621 acctttagta aatgctggag ttggtacatc atttgtcata aatgtatacg cggcacattc
    10681 aaaattataa actgtgcctc ccccagtata tacattcatc atttccatag caatcattga
    10741 ttctggttcc gatgcatagg atctcatatc cctagctctt ccagtttcaa atgtgtttgt
    10801 ataatgtttt tcccaccatt tccatgtatc tgttgatgag ccccagttat cacataagcc
    10861 actcaaccaa aatccactaa cgatagaatc tgtacccgca tcatctctta ttggcgtatt
    10921 tttagttgcc aacaccaaat ttttatgata tttttgactc gcttcaaaga atgtcggatc
    10981 attcataata gtttcccaga accatttttc atgatcatgc cagataaaat gcgctccata
    11041 tttggcacaa acttccaaat atttagcact atgcggagct aactggttat tgtaaatcca
    11101 ataattctca atatttaaaa caccttttaa cacactatac ttttggaatt gttcatctaa
    11161 ccactctgga ggaactgtat tacgctctcc agccgacata ataaccaaga aaactggaat
    11221 gtttagactt tgagcttcct ccaacatctt gacataccat tctctcaatt ctttcgtatc
    11281 tcgtggaata caacttgttg gtttacagac ttttgcagga tgaagttcaa tcgctgcata
    11341 tggctttaca tcttcaggaa tagcttccca tgctcccttc aatgtgttcc cccaccataa
    11401 tccgttacca ttatcattgc catacaacgg agaaagcaac aatggcgatt cattattaat
    11461 cgtcgttctc atttgaacac gattatcagc cttaactcct gctaacaatc caaaacataa
    11521 catcccaata cata
//
LOCUS       DAVGJL010000062        11512 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.062, whole genome shotgun sequence.
ACCESSION   DAVGJL010000062 DAVGJL010000000
VERSION     DAVGJL010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11512
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.062"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            91..744
                     /locus_tag="VVE42_001306"
     CDS             91..744
                     /locus_tag="VVE42_001306"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEU3073493.1"
                     /translation="MEHSSWHALIKAQLPEGYFGKINQFMEQVYSQGIIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANAFLKETGQEPIDWLR"
     gene            754..1218
                     /locus_tag="VVE42_001307"
     CDS             754..1218
                     /locus_tag="VVE42_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEU3073494.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECAAREILEETGLKAKPVLKGVITFPEFTPDLDWYTYVFKVTEFEGDLIDCNEGT
                     LEWVPYDEVLSKPTWEGDHTFVEWLLEDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            1231..2499
                     /locus_tag="VVE42_001308"
     CDS             1231..2499
                     /locus_tag="VVE42_001308"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEU3073495.1"
                     /translation="MLLIKNGRVMDPKSGLDQVCDVLVQDGKIIKIAPEITEEGAETI
                     DATGLVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAVGFSDDGIPLESSKIV
                     KEAMEEAKKLNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALFLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKAPSGMTGLETSLSLS
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFEAGYLAENGPADITIFDAKADRLVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            2513..2773
                     /locus_tag="VVE42_001309"
     CDS             2513..2773
                     /locus_tag="VVE42_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073496.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRTCACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            2784..4145
                     /gene="pdrM"
                     /locus_tag="VVE42_001310"
     CDS             2784..4145
                     /gene="pdrM"
                     /locus_tag="VVE42_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HEU3073497.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPTILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDKIKEGVRLGLPIGGTVFAEVAIFSVVGLIMAKFSSLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASLYGNDPKFIDLTVRFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     SKR"
     gene            4299..5267
                     /locus_tag="VVE42_001311"
     CDS             4299..5267
                     /locus_tag="VVE42_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEU3073498.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGPVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIYAD"
     gene            5283..6275
                     /locus_tag="VVE42_001312"
     CDS             5283..6275
                     /locus_tag="VVE42_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEU3073499.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSIRDKNPVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRR
                     VVQAAEELAEEGISVEIVDPRTLVPLDKDIIINSVKKTGKVVLVNDAHKTSGYIGEIS
                     AIISESEAFDYLDAPIRRCAGEDVPMPYAQNLENAMIPTVESIKDAIRKTYNKE"
     gene            6493..7536
                     /locus_tag="VVE42_001313"
     CDS             6493..7536
                     /locus_tag="VVE42_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEU3073500.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     DTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKVEEVPDNVTPYGEIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRK
                     KVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            7600..9285
                     /gene="lpdA"
                     /locus_tag="VVE42_001314"
     CDS             7600..9285
                     /gene="lpdA"
                     /locus_tag="VVE42_001314"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEU3073501.1"
                     /translation="MPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEA
                     EEDGYLIAILKGDGETVPVTEVIGYLGEERENIPTAGAASPEASSVPVASTSNDDDKS
                     DDAFDIVVIGGGPAGYVAAIKAAQFGGKVALVEKSELGGTCLNRGCIPTKTYLHNAEI
                     IENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVTVHKGIGT
                     ITKDKNVLVNGSELLETKKIILAGGSKVSKINVPGMESPLVMTSDDILEMNEVPESLV
                     IIGGGVVGIELGQAFMTFGSKVTVIEMMDCIVPAMDAEVSKNLRLILERKGMTILTGT
                     KLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYM
                     ETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVA
                     AVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVIADKKYGEILGVHIIGPA
                     AAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKKK"
     gene            9349..10338
                     /locus_tag="VVE42_001315"
     CDS             9349..10338
                     /locus_tag="VVE42_001315"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEU3073502.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     RHQNTIEEINRDYVRENGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEITEYVFS
                     EEELAEINHIKDTKFGTWDWNYGKSPEFNVRRGTKFTSGKVEVFANVTESKIQDIKIY
                     GDFFGIEDVAAVEDVLRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG"
     gene            complement(10403..11473)
                     /gene="xerS"
                     /locus_tag="VVE42_001316"
     CDS             complement(10403..11473)
                     /gene="xerS"
                     /locus_tag="VVE42_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEU3073503.1"
                     /translation="MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSVLENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETEGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LRDLNLKMMVIDVTRKGGKRDSVNVAAFAKPYLENYLAIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVNDEQKNALDSL"
BASE COUNT         3384 a         2161 c         2614 g         3353 t
ORIGIN      
        1 gatttattaa atctttagat aaactgtaaa aagtggtttc catagccact ttttgctata
       61 atagaggcag taaaaatgaa ggagtcggct atggaacact cgtcttggca tgctttgatt
      121 aaggcgcaat tacctgaggg ttatttcggg aaaatcaatc agtttatgga gcaggtctat
      181 tctcagggga ttatttatcc acccaaggaa aaggtttttc aggctctctt gacaacactg
      241 cttgaagaag ttaaggtggt aattctaggg caagacccct atcacggacc aggtcaagcg
      301 cagggcttga gtttttctgt acctgactct atcccagctc caccatcctt gcaaaatatc
      361 ttgaaagaat tgtcagatga tatcggagtt aagaaatctc atgatttgac agcttgggct
      421 gagcaaggag tcttgcttct taatgcttgt ttgactgttc ctgctggaca ggccaatggt
      481 catgctggtc aaatctggga gccttttact gatgctgtga ttcaggtggt caatcatcta
      541 gatagaccag tcgtttttgt actctgggga gcttatgcac gtaagaagaa ggcattagtt
      601 accaatcctc atcacttgat tatcgaatca gcccatccaa gtcctttgtc ggtttataga
      661 ggattttggg gttccaagcc cttttccaag gccaatgcat tcttaaaaga gacaggacaa
      721 gagccaatcg attggcttag ataaggagaa aatatgcctc agttagcgac gatttgctac
      781 attgataatg ggaaagaact gctcatgctc catcgtaata agaaacccaa tgatgtccat
      841 gaagggaaat ggattggtgt gggtggtaag ctagagagag gagagacgcc ccaggaatgc
      901 gcggcgcgtg aaatccttga agaaacaggg ctcaaagcca agccagttct aaaaggtgtc
      961 atcacttttc ctgaatttac accagattta gactggtaca cctatgtttt taaggtgacg
     1021 gagtttgagg gcgacttgat tgactgcaat gaggggacgc tagaatgggt tccctatgat
     1081 gaggttttga gcaagccgac ttgggaaggt gaccacacct ttgttgagtg gcttttagag
     1141 gataaaccct tcttttcagc caagtttgtt tatgatgggg ataaattgtt ggatacccaa
     1201 gttgatttct atgaataaag gagaaagcag atgctactaa tcaaaaatgg tcgtgtaatg
     1261 gatcccaagt ctggtttgga tcaagtttgt gatgtcttag ttcaagatgg gaaaattatc
     1321 aaaattgcgc ctgagatcac ggaagaagga gcagaaacga ttgatgctac tggtcttgtg
     1381 gttgctcctg gtttggtcga tattcatgtg catttccgtg aacctggtca aacacataaa
     1441 gaagacattc atactggtgc cctggcagcc gctgcaggtg gttttactac tgtcgtcatg
     1501 atggctaata ccagtccaac catttcagac gtggagactt tgcaagcagt tctccagtca
     1561 gctgccaaag agaagattaa tgtcaagaca gttgcgacca ttactaaaaa ctttaatggt
     1621 aaaaacttga ctgactttaa ggcgctctta gaagctggtg cggttggttt ctctgatgac
     1681 ggtattccgc ttgagagcag taagattgtc aaggaagcca tggaggaagc caaaaaactc
     1741 aataccttta tcagccttca tgaggaagat ccaggtttga acggtgttct tggctttaat
     1801 gaaaatattg ctagagaaca tttccatatc tgcggtgcta ctggggtggc tgagtacgct
     1861 atgatggcgc gtgatgtcat gattgcctat gcaactaaag cccatgttca catccagcat
     1921 ttgtctaagg aagaaagtgt taaagtagtg gagtttgctc aggggttagg tgcagaagtc
     1981 acagcagaag tagcgccaca gcatttctct aagacagaag cacttttttt aacacaaggt
     2041 agcaatgcta agatgaatcc accgcttcgt ttggaatcag accgtcgtgc cgttatcgaa
     2101 ggtctcaaat caggtgtcat cacagttatt gcgactgacc acgcgcctca tcatgtagat
     2161 gaaaaaaatg ttgaggatat taccaaagcg ccatctggta tgactggctt agaaacatcc
     2221 ctgtctctca gcttgaccta tttagtagaa gctggtgagt tgagcttgat ggaattactt
     2281 gaaaaaatga catacaaccc agccaagctt tacaactttg aagcaggtta cttggctgag
     2341 aatggtccag cagatatcac tatttttgat gccaaggctg accgccttgt ggactcccat
     2401 tttgcttcca aagcagctaa ttcaccattc atcggtgaaa ccttaaaagg gcaggttaaa
     2461 tataccatct gtaagggaca aatcgtctat caagcttgat aggagttaga atatgaatta
     2521 ttttcgaaaa cgtagggaga gacaagcgaa atctaatagt ggaatttact gtcccgcagc
     2581 taatcgttcc aaaattcaat atgaaactaa agaaaaagct gaccatgcgg ttcaatatga
     2641 taacggaggt ttagttagaa gctattattg tcgtacatgt gcgtgttggc atactacttc
     2701 aaaatctaat aaacctccgc taagtttgaa aaactatggt ctgaaactct ttggtttaga
     2761 taaatcagaa tagaaagaga agtatgtata agacaaagtg tttacgagag aagttagtat
     2821 tatttttaaa aattttcttc ccaatcctga tctaccaatt tgccaattat tctgcctctt
     2881 ttgttgatac tgcaatgaca ggtcaataca acactatgga cttggctggt gtatctatgg
     2941 caaccagtat ctggaatcct ttctttacat ttctaacagg gattgtgtca gccttggtgc
     3001 ctatcattgg tcaccatctt ggtcgaggaa aaaaggaaga agttgcgtct gatttttacc
     3061 aatttattta tttggccttg ggcctatctg tggtattgct ggggatggta cttttcttgg
     3121 caccaacaat cttgaatcat attgggttag aagcagcagt agcggcagta gcggttcgct
     3181 atctttggtt tttatctatc gggattatcc ccttgttgct ctttagcgtc attcgttcct
     3241 tgctggattc gctgggcttg accaaactgt ccatgtacct catgcttttg ttactccctc
     3301 tcaatagcgg atttaactat ctcttgattt acggtgcctt tggtgttcca gaactgggag
     3361 gggctggtgc tggtttagga acatccttgg cctactgggt cttgcttggg atttctgttc
     3421 tggttttatt taaacaggag aagctcaaag ccttacacct tgagaaacga attccactta
     3481 atatggataa aattaaggaa ggagttcgtt taggtctgcc tattggggga actgtcttcg
     3541 cggaagtggc tatcttttca gtggttggct tgattatggc taagttttcg tccttgatta
     3601 tagctagtca ccagtcagct atgaactttt caagtcttat gtacgccttt cctatgagta
     3661 tctcatcggc tatggctatt gtcgtttcct atgaagtggg agccaagcga tttgatgatg
     3721 cgaaaaccta tattggtcta ggaagatgga ctgccctcat ttttgcggcc ttcaccttaa
     3781 ccttccttta catttttagg ggaaatgtgg ccagtcttta tggtaacgac ccaaaattta
     3841 tcgatttgac agtgcgtttt ttaacttata gtcttttctt ccagttagca gatacctttg
     3901 cggcgccgct tcagggaatt ttgcgggggt ataaggatac agttattcct ttttaccttg
     3961 gtttgcttgg ttattggggc gtagcaatcc ctgtggctac gctatttgat tctctaacag
     4021 attttggagc ttattcttac tggatcggct tgattattag tttgattgtg agtggggcgc
     4081 tctaccgttg gcgtttaact gtgattatga agagatttga atctttagca aaatccaaac
     4141 gctaaaaagt ttgtgaaaaa atattcttga taagaaaatc ccttgttttc acggggtttt
     4201 cttttttatt ttgtgaaatt tcttttagaa aaactttgac agtatttgcc aaaaacggta
     4261 aaatagtaag gtaagaagaa agttagaaag gcaagaagat gtcaacttta gataaaaatc
     4321 ttttgctaga aatgttccgt aagatggaag aaatccgtcg catggactta aaaattgcac
     4381 aattagtaaa gaaagggaaa gtgccaggaa tgacgcactt ttctgttggt gaagaggcag
     4441 ctaacgtggg ggctatgtta gctctcaatc cagatgatct gattacctca aaccaccgtg
     4501 gacacgggca agctattgct aaagggattg acctcaacgg aatgatggct gaaatccttg
     4561 gtaaatacac tggaacctgt aaagggaaag gtggatctat gcatatcgct gaccttgatg
     4621 ctgggaacct tggtgccaat ggtattgtag gtggtggtat ggggatcgct gtcggtgcag
     4681 ccctcagtca gcaaatgcaa aataccggta aaatcgttgt ctgcttcttt ggagatggtg
     4741 cgaccaatga aggtgttttc cacgaagcag tgaacatggc ttctatctgg aacctgccag
     4801 tcattttcta ttgcattaac aacggttacg gtatctctgc ggatatcaag aaaatgacca
     4861 atatagaaca tatccatcaa cgtagcgccg cttatggaat tcctggaatg ttcatcgaag
     4921 atggtaacaa tgtcatcgat gtctatgaag gatttcagaa agctgtggac catgttcgca
     4981 gtggcaatgg tccagtcttg attgaaagtg taacttatcg ctggctcggt cactcatcat
     5041 ctgaccctgg taaatatcgt acgcgtgaag aagtggaatt gtggaaacaa aaagatccaa
     5101 tcgaaaacct ccgcaattac cttattgaaa ataacattgc aagtgccgaa gaattggaag
     5161 aaatccaagc gcaagtaaag gaagcagtag aagcttctgt taaatttgca gaggaaagcc
     5221 cattcccacc gcttgaatca gcctttgaag atatttacgc agactaagga gaaagagata
     5281 aaatggaaac aaaaacaatg tccttccgtg acaccattat ccttgctatg tctgaggaaa
     5341 tgcgtcgcga tgaaaatgtg ttcttgatgg gagaagacgt cggtgtcttc ggaggagact
     5401 tcggtacttc tgttggaatg cttgaagaat ttggtccaga acgcgtccgt gactgtccga
     5461 tttctgaagc tgccatctct ggagcagcag caggagctgc catgacagga cttcgtccaa
     5521 tcgtcgatat gaccttcatg gacttctctg ttattgccat ggacaatatc gtcaaccaag
     5581 ctgctaaaac acgttacatg tttggtggta aaggtcaggt tccaatgact gttcgttgtg
     5641 cagctggtaa cggagttggc tctgccgccc agcactcaca atctctagag tcttggttta
     5701 ctcacattcc aggacttaag gttgtggcac ctggaacacc tgcggacatg aaaggcctgc
     5761 ttaagtcttc tatccgtgat aaaaacccag ttattatact tgagtacaag tcagaattta
     5821 accaaaaagg ggaagtgcca gttgatccag actacacaat tccacttggg gttggtgaaa
     5881 tcaaacgcca aggaacggat gtaacagttg ttacttatgg aaaaatgctt cgccgtgtgg
     5941 ttcaagctgc tgaagaatta gcagaagaag gaatttcagt tgaaattgtt gacccacgta
     6001 cccttgttcc gcttgataag gatatcatca taaactcagt gaagaagact ggtaaggttg
     6061 ttctggtcaa cgatgcccac aaaacaagtg gctatatcgg tgaaatttca gctattattt
     6121 cagaatcaga agcatttgac tatctagatg caccaattcg ccgttgtgca ggagaagatg
     6181 tgccgatgcc ttatgcacaa aacctagaaa atgcaatgat tccaacagtt gaaagtatca
     6241 aagatgcaat ccgaaaaact tataacaaag aatagagtta cataagataa gttatgtaaa
     6301 taaaataaag ctgacgagaa actttgtatc ttttaggtgc agagctcttc gtcattaata
     6361 tcttagatta gagcacgggc taaaagctcg gaaaaaagat aaatctcctt ggcttcatcg
     6421 aagccatcgt cgatttccta tttttcagtc gcttttgacg ccctttgtat cataaataga
     6481 attggagagg tcatggctga tgacaagcta agagcgactc ctgcagctag aaagttagcg
     6541 gatgatttag ggataaacct ctacgacgtt tctggctcag gtgcaaacgg tcgtgtccac
     6601 aaagaagacg tggaaactta taaagacaca aacgtggttc gcatttcgcc acttgcaaaa
     6661 cgaattgccc tcgaacataa cattgcttgg caggaaatcc aaggaaccgg tcatcgtggt
     6721 aaaatcatga agaaggatgt tttggccctg cttcctgaaa atatcgaaaa cgatagcatc
     6781 aaatctcctg ctcagattga aaaagtggaa gaagtccctg ataacgtaac accatatggt
     6841 gaaatcgagc gtattccaat gacaccaatg cgtaaggtta ttgcccaacg catggttgaa
     6901 tcttacttga ctgcgccaac cttcaccctc aactatgaag ttgatatgac tgaaatgttg
     6961 gctcttcgta agaaggttct tgagccaatc atggaagcaa ctgggaagaa gactactgta
     7021 acagaccttc tttcgcttgc agttgttaag actcttatga aacacccata catcaacgct
     7081 tcattgacag aagatggcaa gactattatc actcacaact atgtcaatct tgccatggca
     7141 gttgggatgg ataatggatt gatgacacct gttgtttaca atgctgagaa aatgagtctt
     7201 tcagaactgg ttgtagcctt taaggatgtt attggccgta ccttggatgg taaattggct
     7261 ccaagtgagt tacaaaattc aacattcaca atcagtaatt tgggaatgtt tggtgttcag
     7321 tcctttggtc cgattattaa ccaacccaac tcagctatcc ttggtgtcag ttcgacaatc
     7381 gagaagccag ttgtcgtcaa tggtgaaatt gtgattcgcc caatcatgag tttaggatta
     7441 accattgacc accgtgtcgt agatggtatg gctggtgcta agtttatgaa ggacttgaaa
     7501 gagttgattg aaacgccaat ctcaatgtta atttaagagc aagagtattc atttttaaga
     7561 tatagataaa ggaaggaaga catggcctta gaagtaatta tgccaaaagc cggcgtggat
     7621 atgacagaag gacaaatcgt ccaatggaat aaaaaagtcg gagaatttgt aaaagaagga
     7681 gaaatccttt tggaaatcat gactgataaa gtcagcatgg aattggaagc cgaagaagat
     7741 gggtacttga ttgccattct caaaggagat ggtgaaactg tccctgtaac ggaagttatc
     7801 ggttaccttg gtgaagaaag ggaaaacatc ccaacagctg gagcagcatc gccagaagct
     7861 agctctgtac ctgtagcaag tacctcaaac gatgatgata agagcgatga tgcttttgat
     7921 atcgttgtga ttggtggagg tcctgctggt tatgttgcag ccattaaagc tgcccaattc
     7981 ggcggtaagg ttgcccttgt tgagaaatct gaacttggtg gaacctgctt aaaccgtgga
     8041 tgtattccaa ccaagaccta ccttcataac gctgaaatta ttgaaaatat cggtcatgct
     8101 gcaaatcgtg gtatcgtgat tgaaaatcct aacttcactg ttgatatgga aaaactttta
     8161 gaaactaaat ctaaagttgt taatactctt gttggtggtg ttgcaggact tcttcgtagc
     8221 tacggagtta ctgttcataa aggaattggt acaatcacta aagacaagaa cgtcttggta
     8281 aatggctctg aattgcttga aaccaagaaa atcattcttg ctggtggttc aaaagtcagc
     8341 aagatcaacg tccctggtat ggaatctcca cttgtcatga ctagtgatga cattcttgaa
     8401 atgaacgaag tgccagaaag ccttgttatc atcggtggtg gagttgtcgg tatcgaactc
     8461 ggtcaggcct tcatgacatt tggttcaaaa gtgactgtta tcgaaatgat ggactgtata
     8521 gttccagcta tggatgcgga agtttctaag aaccttcgct tgatccttga gcgtaaagga
     8581 atgaccatct tgactggtac taaactgcaa gaaatcattg aggaaaatgg tcaacttcgt
     8641 atcaaggttg aaggaaaaga cgatatcatc gcaagcaaag ctcttctttc aattggtcgt
     8701 atgccagacc ttgaaggtat tggagaggtt gagtttgaat tggatcgtgg ttgtatcaag
     8761 gtcaacgaat acatggaaac ttcagttcca ggcatttatg caccaggtga catcaacggt
     8821 actaagatgt tggctcacgc agctttccgc atgggtgaag tttccgctga aaatgccctt
     8881 aaaggaaatc atgcagttgc caaattgaat ttgactcctg cagccatcta cactctccct
     8941 gaagtagcag cagtaggttt gacagaagaa caagcccgtg agaaatacga tgttgccatc
     9001 ggtaagttta actttgctgc taacggtcgt gctattgcat ctgacgctgc tcaaggtttc
     9061 gtaaaagtta tcgctgataa gaaatacgga gaaatccttg gtgtacacat tattggtcct
     9121 gcagccgcag aattaataaa cgaagcatca agcatcatcg aaatggaaat cactgttgag
     9181 gaaatgctga agaccatcca cgggcaccca acttactctg aagtgatgta cgaagcgttt
     9241 gcagatgttc taggaatggc catccattca cctaagaaaa aataaaacga agatagatta
     9301 gactggaagg ttatttccag tttctatcat ataaagtaaa gggatatcat gaaatacatt
     9361 atcaatcatt caaatgacac tgcttttaat attgccttgg aagaatacgc ctttaaacac
     9421 cttttggatg aggatcaaat cttcctactt tggattaaca agccatctat cattgttggt
     9481 cgtcaccaga acactatcga agaaatcaac cgtgattatg ttcgcgaaaa tggtattgag
     9541 gtagttcgcc gtattagtgg tggtggagct gtttaccacg atttaaataa cctcaactac
     9601 acgatcatct ccaaagaaga tgaaaataag gcatttgact tcaagagctt ctcaactccg
     9661 gttatcaata ccctggctca acttggggtt aaagctgagt tcacaggtcg taatgacctt
     9721 gagattgatg gtaagaagtt ctgtggcaat gcccaagcct atatcaacgg ccgtatcatg
     9781 caccacggtt gcttgctctt tgacgttgat ttgtcagtcc tcgcaaatgc ccttaaggtt
     9841 tcaaaagata aatttgaatc aaaaggtgtg aaatccgtcc gtgcccgtgt aaccaatatt
     9901 atcaatgaat taccaaaaaa aatcacagtc gaaaaattcc gtgatttact cttggaatac
     9961 atgaaaaaag agtacccaga gattactgaa tacgtctttt cagaagaaga attggccgaa
    10021 atcaatcaca tcaaggatac taagtttgga acttgggact ggaactatgg taaatcacct
    10081 gaatttaacg tccgtcgtgg aaccaaattc actagtggta aggttgaagt ctttgctaac
    10141 gttactgaat caaaaatcca agacatcaag atttatggtg acttctttgg tattgaagac
    10201 gttgctgcag tagaagatgt ccttcgtggg gtgaaatacg aacgtgaaga tgttcttaag
    10261 gcgctaaaaa ccattgatat cacacgctac ttcgctggta ttagccgtga agaaatcgct
    10321 gaagcggtag ttggataaat caaaaagaag ttggccaact tctttttttg atatttattt
    10381 acataattta aaatacgtaa aattataaac tatccagagc attcttttgt tcatcattaa
    10441 caatatgggt atagaggtca gtgacttgtg tgctggcatg tcctagttgg tgactgacta
    10501 aaacttgtga tttagtcgca tcatagagcc tagttgctag tgtatggcgc agtttatggg
    10561 gtgttacacg tactttaaaa tcctctgagt atttagcaac cattttctca acgctagaag
    10621 catcgatacg attaggaaca cctctgtaga gagttaaaaa aagggctgta tctgtttttt
    10681 ccgttttata gcgttgattc cgaatggcca gataattctc taaataaggt ttagcaaaag
    10741 cagcgacatt gactgagtca cgtttgccac cttttcgagt aacatcaata accatcattt
    10801 ttagattgag atctcttaga tctagattaa cagcttcaga taagcgaaca ccagatgcca
    10861 agagaagggc aataatggct aaatctcgtt ctttattttt gttgaatgat gagagagctc
    10921 gatttgaaag ctgttgtggg tgctcttgat cgatataagt tagaaaacct tctgtttcat
    10981 cacctagaaa gagtttttgc ttgatatttt cagctctggc agcaagggtt tctttctttt
    11041 tcttggttga aacttttttc attacattac gatagaaata aggttccccc tgatcgtttt
    11101 caacctcctc ggttagatat ttgtaaagac tagaaagtgc tgataaggtt cgattgatag
    11161 ttgtctgtga aacaccttgt tttgttgtat tagcattcag caagggacgt tcacgtagat
    11221 aaaggataaa ggattccatg tctttcttag acatattttc caaaactgat aaaggaatat
    11281 cggatatttt atcagcgttt gaaataccag actccaaaac ccagctgaaa aatcggtcat
    11341 attccttaag gtattcgtac agggttgtaa aactgtaggg cacagccagc ttagattggt
    11401 agtattccag aacataccag ggcatgagtt gttttagttt gtcgattcgt tccagtaaaa
    11461 tctcacgttt catgtatttt ctccataagt aagtatacta gtagtatagc at
//
LOCUS       DAVGJL010000063        11356 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.063, whole genome shotgun sequence.
ACCESSION   DAVGJL010000063 DAVGJL010000000
VERSION     DAVGJL010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11356
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.063"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            12..572
                     /locus_tag="VVE42_001317"
     CDS             12..572
                     /locus_tag="VVE42_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEU3073504.1"
                     /translation="MTWIILGVIALIVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNVKLETFPSNIIAGMFG
                     FKAADFLQTPEEEKSVPKVDFSGLGD"
     gene            574..1473
                     /gene="htpX"
                     /locus_tag="VVE42_001318"
     CDS             574..1473
                     /gene="htpX"
                     /locus_tag="VVE42_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEU3073505.1"
                     /translation="MLFDQIASNKRKTWILLLVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRVFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALVSAITMLSGMAGRMMWWGGAGRRRSDDDRDGNGLEIIMLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINDPKKG
                     GGFQKLFYTHPPISERIERLKQM"
     gene            complement(1622..3181)
                     /locus_tag="VVE42_001319"
     CDS             complement(1622..3181)
                     /locus_tag="VVE42_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU3073506.1"
                     /translation="MHYEHSRKGNHMIKINHLTITQNKDLRDLVSDLSMTIQDGEKVA
                     IIGEEGNGKSTLLKILMGEALSDFTIKGNIQSDYQSLAYIPQKLPEELKKKTLHDYFF
                     LDSIDLDYSILYRLAEELHFDSNRFASDQKIGNLSGGEALKIQLIHELAKPFEILFLD
                     EPSNDLDLETVDWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLV
                     EHLDYDSYSEQRKANFAKQSQQAANNQRAYDKTMEKHRRVKQNVETALRATKDSTAGR
                     LLAKKMKTVLSQEKRYEKAAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLS
                     IDDRVLVQKLQLTVRGQEKIGIIGPNGVGKSTLLAKLKQLLNDKREISLGFMPQDYHK
                     KLQLDLSPIAYISKTGEKEELQKIQSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDL
                     VLRKPNFLLLDEPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRFLKEICSIIYRMTE
                     HGLKLVNLEDL"
     gene            complement(3420..3737)
                     /locus_tag="VVE42_001320"
     CDS             complement(3420..3737)
                     /locus_tag="VVE42_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEU3073507.1"
                     /translation="MEDKKLIQLLSKLNKSYQNCKQGTADDIRLQELLNTTMQELKKT
                     EKLNNSILIDLEKFYQPTSLLIGLGSLKLNDQARTAWRNYDKFHYEHVKHVLSLYGPV
                     FGF"
     gene            3907..4449
                     /locus_tag="VVE42_001321"
     CDS             3907..4449
                     /locus_tag="VVE42_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEU3073508.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVDDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNQLDQEVLDDD
                     LVLPIENGELDLAESVSDNILLNIPIKVLTAEEEAGQGFISGNDWQIMTEEEYQAQKA
                     VKKEENSPFAGLQGLFDGDE"
     gene            4449..5078
                     /gene="nth"
                     /locus_tag="VVE42_001322"
     CDS             4449..5078
                     /gene="nth"
                     /locus_tag="VVE42_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEU3073509.1"
                     /translation="MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPGLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            5289..5810
                     /gene="pyrR"
                     /locus_tag="VVE42_001323"
     CDS             5289..5810
                     /gene="pyrR"
                     /locus_tag="VVE42_001323"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEU3073510.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            5829..6752
                     /locus_tag="VVE42_001324"
     CDS             5829..6752
                     /locus_tag="VVE42_001324"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEU3073511.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGAELFFAGPEEWRSQEFADYGKFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            6802..7881
                     /gene="carA"
                     /locus_tag="VVE42_001325"
     CDS             6802..7881
                     /gene="carA"
                     /locus_tag="VVE42_001325"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEU3073512.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            8194..>11356
                     /gene="carB"
                     /locus_tag="VVE42_001326"
     CDS             8194..>11356
                     /gene="carB"
                     /locus_tag="VVE42_001326"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEU3073513.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKERPDALLPTLGGQTGLNMAMEL
                     SKNGILDELGVELLGTKLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAVAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTYAMFEPSLDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLETGVHHNEIPELAAVSDDALIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKTAKLNGFSDRKIAELWGTTDDKVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIKSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAALAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKNVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFV"
BASE COUNT         3238 a         2248 c         2517 g         3353 t
ORIGIN      
        1 ggacttatat catgacttgg attattcttg gagttatcgc tcttattgtt atttttgtga
       61 ttgttagcta taacggtttg gttaaaaatc gtatgcaaac caaggaggct tggagtcaga
      121 ttgatgttca gttgaaacgt cgcaatgatc tcttgccaaa cttgattgag actgtaaaag
      181 gttatgccaa atatgaaggt tctacccttg aaaaggtggc agaactacgt aaccaagtgg
      241 cggcagcgac ttcaccagca gaagctatga aagccagtga tgccctcact cgtcaggttt
      301 caggtatttt tgcagttgca gaaagctatc cagatttgaa agctagtgct aactttgtta
      361 aattgcaaga ggagttgaca aacacagaaa ataaaatttc ttactctcgt caactctata
      421 acagtgttgt cagcaactac aatgtaaaat tagaaacttt cccgagcaat attatcgctg
      481 gaatgtttgg atttaaagcg gcagatttcc ttcaaacacc tgaagaggaa aagtcggttc
      541 ctaaagttga ttttagcggt ttaggtgact aagatgttgt ttgatcaaat tgcaagcaat
      601 aaacgaaaaa cctggatttt gttgctggta tttttcctac tcttagctct tgttggttat
      661 gcggttggtt atctctttat aagatctgga cttggtggtt tggttatcgc actgattatc
      721 ggctttatct acgctttgtc tatgattttt caatcgacag agattgtcat gtccatgaat
      781 ggagcgcgtg aggtggatga gcaaacggca ccagacctct accatgtagt ggaagatatg
      841 gctctggtcg ctcagattcc tatgccccgt gttttcatca ttgatgatcc agccttaaat
      901 gcctttgcga caggttctaa tcctcaaaat gcggctgttg ctgcgacttc aggtctacta
      961 gctatcatga atcgtgaaga actagaagct gttatgggac atgaagtcag tcatattcgt
     1021 aattatgata tccgtatttc gactattgca gttgcccttg ttagtgctat caccatgctt
     1081 tctggtatgg caggtcgtat gatgtggtgg ggtggagcag gtcgcagacg aagtgatgat
     1141 gaccgagatg gaaatggtct tgaaatcatt atgctagtgg tttccctact agctattgta
     1201 ctggcacctc tcgctgcaac cttggttcag ctcgctattt ctcgtcagag ggaatttctg
     1261 gcagatgcat ctagtgtcga gctgactcgc aatccccagg gaatgattaa tgccctagat
     1321 aagttggaca atagcaaacc tatgagtcgc cacgtcgatg atgctagcag tgctctttat
     1381 atcaatgatc ccaagaaagg tggggggttc caaaaactct tttataccca cccacctatc
     1441 tcagaacgga ttgaacgttt aaaacagatg taaaatgaag gctggaaaaa agtctttaaa
     1501 atctgaaaaa cgcataatat caggtgtgaa aacttgatat tatgcgtttt actatgggaa
     1561 gatttacttc tttttctcct aaaattatgt ttttgcccca cctatctgct atgttgcaaa
     1621 tttataaatc ttctaaatta actagcttca aaccgtgttc tgtcatgcga tagatgatcg
     1681 agcagatttc ttttaagaaa cgacggtcat gcgaaacagt gatgagaccg cctggatagg
     1741 ttgcaaagag ttttctgatt tggggttgag aagtgggaga aaagtttcgt gtgggttcat
     1801 ccagcaggag aaagtttggt ttgcgcagga ctaaatccaa aagcaggagt tttccctgtt
     1861 gtccgccaga taaggagcga atttgatgct gcatttctgg ataactgaaa ttgagactgg
     1921 ctaggtgaga ttggattttc tgaagttcct ctttttcccc agttttgctg atataagcta
     1981 ttggtgataa atccaattgc agttttttgt ggtaatcttg tggcataaaa ccaagggaaa
     2041 tctctctttt atcattcaga agttgcttta atttggctaa cagagttgat ttcccaacac
     2101 catttggccc gataataccg attttttctt gcccacggac agttagttgt agtttttgaa
     2161 ccaaaactcg gtcgtcaatg gacaaatttt ctttttccag ttggactaag actttagaag
     2221 ctggtaatgg ttggatgtct gaaaagaaaa gttggatttg ttcctcttca agtggctttt
     2281 gagtcatgga ctgagctgcc ttttcgtagc gtttttcttg tgagaggaca gttttcatct
     2341 ttttagccaa taggcgaccg gcagtactat ctttggtagc tcgaagcgca gtttctacat
     2401 tttgcttaac tctccgatgt ttttccatgg ttttatcgta ggctctttgg ttgttagcag
     2461 cttgctgact ttgtttggca aaattagcct ttctctgctc actatagcta tcatagtcta
     2521 aatgctctac tagcgtttcc gcttctttac ggtgtttgac cagtcgcaag tgaacaatag
     2581 tgtctgctgt ttcagaaaga aagtcttcat catgggaaat gaaaataacg gtttgcctgg
     2641 tcttttgaat ctggcctttt agccaatcaa ctgtctcaag gtctaggtca tttgaaggtt
     2701 catctaaaaa tagaatctca aagggtttgg ctaactcatg gataagctga attttcaaag
     2761 cttctccccc tgatagattg ccaatctttt ggtcacttgc gaaacgattg ctatcaaaat
     2821 gcaattcctc cgccaaacga tagaggatac tgtagtctaa atcaatagaa tctaaaaaga
     2881 agtagtcgtg taaagttttc tttttcagct cctcgggaag tttttgagga atgtaggcca
     2941 gtgactgata gtcagattgg atgtttccct tgatagtgaa atcagacaaa gcttccccca
     3001 ttaaaatttt aagtaaggtt gatttgccat ttccttcttc accaataata gcaacctttt
     3061 ccccgtcttg gatggtcatg cttaggtcag agacaaggtc tcgtaaatct ttgttttgtg
     3121 tgatggttag atgattgatt tttatcatat gatttccctt tctagaatgt tcatagtgca
     3181 taacaaaaag ctctacttta cctagtagag cccaaagtct atgtcaaaaa aactctatta
     3241 acatgccaga aaaagagcat aaaagagcac acaaaaagag acaaaaacaa gatctactaa
     3301 ataaaggttc tttatttgat agacttagtt gttcatgtct cccttacctc cgagtattag
     3361 tatctttata ttataccttt aaggaaattt tgtcaacaga aaactggaaa ttctatgctc
     3421 taaaatccaa aaacaggtcc atagagactt agtacgtgtt tgacatgctc gtaatggaat
     3481 ttatcatagt ttcgccaagc agtgcgtgct tgatcgttta gttttaggct acccagtcca
     3541 atcagaagac tggtgggttg gtaaaatttc tcaagatcaa ttaagatact gttgttcaac
     3601 ttttccgttt ttttgagctc ttgcatagta gtgtttagca gctcttgtag tcgaatatca
     3661 tctgccgtac cctgtttaca gttttggtag cttttattta acttggatag gagttgaatg
     3721 agtttcttgt cttccatgtg agcatcctcc ttgatctgat gtactctttc cctatagtat
     3781 agcactaaaa aacaaaactg tcatagtaaa tatgttaaaa ttctgaattt taattacaaa
     3841 gtttggtgtg ctgatttgta gccttttcat ggtataatca agagagtaaa tctttatgga
     3901 ggaagtatga aattaaatat tcaagaaatt cgtaagcagt ctgaaggttt gaactttgaa
     3961 caaacgttag atttagttga tgacctgcgt gcacgtaatc aagaaatttt agatgtaaaa
     4021 gatatccttg cagttgggaa agtacaatat gaagaccgta tgtatttctt agattatcaa
     4081 ctatcttata ccattgttct tgcttcgagt cgcagtatgg agccagttga gttagttgaa
     4141 tcttatccag tcacggaagt tttcatggaa ggcgcaacta accagctaga tcaagaagtt
     4201 ttagatgatg acttggtctt gcccatcgaa aatggggagc ttgaccttgc tgagagtgta
     4261 tcagacaata tcctgctaaa cattcctatc aaggtcttga cggctgaaga agaagctggt
     4321 caaggattta tctcaggaaa tgactggcaa atcatgacag aggaagaata ccaagctcaa
     4381 aaagcagtaa agaaagaaga aaacagtcct tttgctggct tacaaggact atttgacgga
     4441 gatgaataat ggtcttgtca aaaaaacgag cacgaaaggt gctagaagaa atcattgccc
     4501 tcttcccaga tgccaagcct agtcttgatt ttaccaatca ttttgaactc ctggttgcgg
     4561 tcatgttatc agcccagacg acagatgcag cggtaaataa ggccacacca ggtctctttg
     4621 ttgcctttcc aacaccccaa gctatgtctg tagcgacaga gagtgagatt gcttcacata
     4681 tttctcgctt gggattgtat cggaataaag ctaaattcct taaaaaatgt gcccaacagt
     4741 tactagacga ttttgatggt caagtccctc agacacgtga agaattggag agtttggcag
     4801 gtgttggtcg caagacagcc aatgttgtca tgagtgtagg atttgggatt ccagcctttg
     4861 cagtggatac tcatgtggag cgtatttgca aacaccacga tattgtcaaa aaatcagcga
     4921 cgccacttga ggtggaaaag cgggtcatgg atatcttgcc gcctgagcag tggttagctg
     4981 cccatcaggc catgatttat tttggaagag ccatttgcca tccaaaaaat ccagagtgtg
     5041 accagtaccc acaattatat gattttagca atttgtaaga tgctacattg aaactttttg
     5101 cttgatttta agcatgcttt gtgctatagt aaatgataac taaatattcc tttaaacctg
     5161 tcccgtgagg caggcaagga gcgcgataaa gtagaagggt gaagtctata ctgtgtgcag
     5221 tagggctcgc ttggctataa atttcaagtc tccttgttta aggagacttt tctttttgga
     5281 ggtttgtcat gaaaacaaaa gaagttgtag acgaattgac cgtcaaacga gcgattacgc
     5341 gtattactta tgagattatc gaacgcaaca aagatttgaa taagatcgtc ttggctggta
     5401 ttaaaactcg tggtgtcttt attgcccacc gaatccaaga acgtttgaag cagttagaaa
     5461 atctttcagt tcctgttgtg gaattggata ctaaaccttt ccgtgatgat gttaaaagtg
     5521 gagaagatac ttctttggtt tctgtcgatg tgacagaccg cgaagttatc ttggtggatg
     5581 atgtgctcta tacaggtcgt accatccgtg ctgctattga taatattgtc ggtcatggtc
     5641 gtcctgcgcg tgtgagttta gcagttctag tcgatcgtgg acatagagaa ttgccaatcc
     5701 gtccagatta cgttggaaaa aatatcccaa ccagtcgttc tgaagaaatc atcgtagaga
     5761 tgacagaact tgatgaccaa gacagagttc tgattactga agaagcttag aaagttaaag
     5821 gagtagccat gtcagaaaat caacaagcat tgaaccatgt ggtgtccatg gaagacctca
     5881 ctgtcgatca agtaatgaaa ttgatcaagc gaggaattga gtttaaaaat ggagcccagc
     5941 ttccctatga agaccatccg attgtttcca atctcttctt tgaggattct acacggacac
     6001 ataagtcctt tgaagtcgca gagattaaac ttggattgga acgacttgac tttgatgtga
     6061 agactagttc ggttaataag ggtgagacac tttatgacac cattttgact ctgtctgctt
     6121 taggagtgga tgtctgtgtg attcgccatc cagaggtcga ctactataga gaattgattg
     6181 ctagtccaac gattacgact tccatcatca atggtggaga tggttcgggc caacacccta
     6241 gccagagctt gcttgatttg atgaccattt atgaggaatt tggccacttt gagggtctta
     6301 aagttgctat tgcaggtgac ttggaccact cacgcgttgc caaatccaat atgcagattt
     6361 tgaaacgctt gggagctgaa ctctttttcg ctggacctga ggaatggaga agtcaagagt
     6421 ttgcagacta tggaaagttt gtaaccattg atgaaatcat tgatcaggtt gatgtcatga
     6481 tgtttctccg tgtgcaacac gaacgccatg atagtggagc tgtattttca aaagaagact
     6541 atcatgccca acatggcttg actcaagaac gttacgatcg tttgaaagaa acagcaatcc
     6601 tcatgcaccc agctccaatc aatcgtgatg tagaaatagc agaccacttg gttgaagcac
     6661 caaaatcacg gattgtccaa caaatgacca atggtgtctt tgttcgaatg gcaatcttag
     6721 aatccgtact agcgagtaga aacgccaact aaaacacaag acgttaaaag acgccagtga
     6781 gcgtccacga gaggtgaaaa tatgacaaaa agaattctag tattagaaga tggcacagtt
     6841 tttgaaggca aggccttcgg agcagatatt gatgtaacag gcgaaatcgt ctttaataca
     6901 gggatgaccg gctaccaaga atccattaca gaccagtctt ataatggaca aatcttgacc
     6961 tttacttatc ctttggtagg aaattatggt attaatcgtg atgattatga atccattatt
     7021 ccaacttgta agggagtcgt tgtttttgaa gaagcgcgta gagctagcaa ctggcgcaac
     7081 caaatgacct tggatgaatt tttgaaagcc aagaaaattc cagggatttc aggaattgat
     7141 acgcgtgctc ttaccaagat tatccgtaag catggtacta tgcgtgcaac cttgacccat
     7201 gttggggaca gtatggacca tgtgacggac cagctccaag caacagtttt accgacagac
     7261 aatatcaaac aggtttctac taaaacgtct tatccagctc caggagttgg tttgagcgtg
     7321 gtgctagtgg actttggtct caagcactca atcctacgtg aactttctaa acgcaactgt
     7381 aacgtgacag ttgttcctta ttcgacaacg gctgaagaaa ttctccatct caatcctgac
     7441 ggagttatgt tgtcaaatgg tccaggtaac ccagaagacg ttccacaagc actcgacatg
     7501 attcgcggtg tacaaggaaa aattccaatc tttggtattt gtatgggaca ccaactcttt
     7561 gcaatggcaa acggggctaa gacctataag atgaagtttg gccaccgtgg attcaaccat
     7621 gcggtacgtg aaatcgcaac aggacgagta gacttcacca gccagaacca tggttatgca
     7681 gtcagccgcg aggatttgcc agagcacttg attattaccc acgaagaaat caatgacaag
     7741 tcagtggaag gtgtgcgcca cagataccaa cctgctttct ctgttcaata ccacccagat
     7801 gcagctcctg ggccacacga cgctagctac ctatttgacg agtttatcga gatgatggaa
     7861 gtttttaaac aatcaaacta agaacgagta aaagctcgtc ggagaattta tgtaactgaa
     7921 cacggacgga aagctcagaa tgtagagaaa tcaacttgga ttgtcaatcc ttccttgatt
     7981 ttctaaaatt caatcgcttt ctattcgtcc tttgtatctt atttaatgtc gccctgagag
     8041 gcgacgaata gaaaggagaa gggtgctccg tatttgctga actgaatacg ggctacggac
     8101 ggtgctaaaa agatagttat tcctagaact gacagttctt cgtcataact cctattttag
     8161 ctttgtccgc ttgacgccct taatatctta tatatgccta aacgtactga tattcaaaaa
     8221 attatggtga ttggttctgg tccgattatt attggccagg ctgctgagtt tgactacgct
     8281 gggactcagg cttgcttgtc gttgaaagag gaaggttatg aggttgtttt ggttaactca
     8341 aatcctgcaa ccatcatgac ggacaaggaa attgctgaca aggtttacat tgaaccgatt
     8401 acacttgagt ttgtgacacg tattcttcgt aaggaacgtc cagatgcctt gctaccaaca
     8461 ctcggtggtc agacaggtct caacatggcc atggaattat ctaaaaatgg tatcctagac
     8521 gagcttggcg ttgaacttct gggtaccaaa ttatctgcca ttgaccaagc ggaggaccgt
     8581 gacctcttta aacaattgat ggaagagctt gaacaaccca ttccagaatc tgaaattgtt
     8641 aacacagtag aagaagcagt tgcctttgca gcgacaattg gctacccagt catcgttcgt
     8701 ccagccttta cccttggtgg tactggtggt ggtatgtgtg ccaacgagaa agaattgcgt
     8761 gaaatcactg aaaatggttt gaaattgtca cctgttaccc aatgtttgat tgagcgttcg
     8821 attgccggtt tcaaggaaat tgaatacgaa gtgatgcgtg actcagctga caatgccttg
     8881 gttgtttgta acatggaaaa ctttgaccca gttgggattc acacagggga ttccattgta
     8941 tttgcccctg cgcaaaccat gtcagactat gaaaaccaaa tgcttcgtga tgcgagcttg
     9001 agcattattc gtgccctcaa gattgaaggt ggatgtaatg ttcagctagc tcttgatcct
     9061 aacagcttca agtattatgt tatcgaagta aaccctcgtg tatcacgttc gtcagccctt
     9121 gcttctaaag cgactggata tccgattgct aagttggctg ccaagattgc agtaggtttg
     9181 accttggatg aggttataaa cccagttaca ggttcaacct atgccatgtt tgaaccatcc
     9241 cttgactacg tagttgctaa gattccacgt ttcccatttg acaagtttga aaagggtgaa
     9301 cgccgtctcg gtacacaaat gaaggcgact ggagaagtca tggcaatcgg tcgtaatatc
     9361 gaggaatctc ttctcaaggc ctgccgctcc cttgaaactg gggtgcatca caatgaaata
     9421 cctgaacttg cagcagtttc tgatgatgcc ttgattgaaa aggttgtcaa agcccaagat
     9481 gaccgtctct tctacgtatc agaagccatt cgtcgtggct acacacctga agaaattgca
     9541 gagcttacaa aaattgatat cttctatctt gataaactct tgcatatctt tgaaattgag
     9601 caagaattgg gtgctcatcc acaagatcta gaagttttga aaacagccaa acttaatggt
     9661 ttttcagacc gtaagattgc tgaactctgg ggaacgactg atgataaagt tcgccaactt
     9721 cgcttggaaa acaagattgt tccagtttat aagatggtag acacttgtgc ggcagagttc
     9781 gattccgaaa cgccatattt ctattcaacc tatggttggg aaaatgaatc tatcaaatct
     9841 gataaagaat ctgtacttgt cctaggttca ggtccaatcc gtatcggtca aggggttgag
     9901 tttgactacg caaccgttca ctctgttaag gctatccagg cagctggtta tgaagctatc
     9961 atcatgaact caaacccaga gaccgtttca acagacttct ctgtatcaga taagctttac
    10021 tttgagccat tgacattcga agatgttatg aacgtcattg acttggaaca accaaaggga
    10081 gttatcgttc agttcggtgg tcaaacagcc atcaaccttg cggagccatt ggcaaaagca
    10141 ggtgtgacca tccttggtac acaggttgct gacctagacc gtgccgaaga ccgtgacctc
    10201 ttcgagcaag ctctcaaaga attggatatt ccacagccac caggacaaac ggctaccaat
    10261 gaagaagaag cagctcttgc agctcgtaag attggcttcc cagttcttgt ccgcccatct
    10321 tatgtacttg gtggacgtgc catggaaatc gttgaaaacg aagaagacct tcgttcttac
    10381 atgcgaactg ctgttaaggc tagtccagac catccagttc ttgtcgactc ttatatcgtt
    10441 gggcaagagt gcgaagttga tgccatttca gatggtaaaa atgtactcat tcctggtatc
    10501 atggagcata tcgaacgtgc tggtgtccac tcaggtgact caatggctgt ttacccacca
    10561 caaaccttgt cgcaaaaggt tcaggagacc atcgcagact acaccaaacg cttagcaatc
    10621 ggtcttcact gccttggaat gatgaacatc cagtttgtta tcaaggatga aaaagtctac
    10681 gttattgagg tcaatccacg tgccagccgt accgttccat tcctatctaa ggtaaccaat
    10741 attcctatgg ctcaagtagc gaccaagctc attcttggtc aaagtttatc agaacttggc
    10801 taccaaaatg gactttaccc tgaaagcacc cgtgttcata tcaaggcacc tgtcttctcc
    10861 tttaccaaac tagctaaggt agacagcttg ctcggtcctg aaatgaagtc aacaggtgaa
    10921 gttatgggtt ctgatgctac tttggaaaaa gctctctata aagcctttga agcttcttat
    10981 ctacacttgc caacttttgg taatgtcgtc tttaccatcg ctgatgatgc caaagaagaa
    11041 gccttgaact tggctcgtcg tttccaaaac attggttatg gaatccttgc gacagaaggg
    11101 acagcagcct tctttgctag tcatggcttg caagcccaac ctgttggtaa gattggtgat
    11161 gacgataagg atatcccaag ttttgtacgc aaaggaagaa ttcaagctat cattaacaca
    11221 gtaggaacaa aacgaactgc tgacgaagat ggtgagcaaa ttcgtcgttc agccattgaa
    11281 cacggggtgc ccctcttcac agcgctagat acagctaatg ccatgcttaa ggtacttgaa
    11341 agtcgtagct ttgtta
//
LOCUS       DAVGJL010000064        11158 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.064, whole genome shotgun sequence.
ACCESSION   DAVGJL010000064 DAVGJL010000000
VERSION     DAVGJL010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11158
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.064"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            71..451
                     /locus_tag="VVE42_001327"
     CDS             71..451
                     /locus_tag="VVE42_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073514.1"
                     /translation="MKLKRLALISGIVGLVGGILLLIGPFVLLGTAVNTAATTLNGGA
                     TAGAFSGVALLLNALKIANLVLGIIAIVYYKGDKRVGAAPSVLMIVSGGVSLIPFLGW
                     VGGILAIIGGSLFLATLKKFKSEE"
     gene            985..1677
                     /locus_tag="VVE42_001328"
     CDS             985..1677
                     /locus_tag="VVE42_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEU3073515.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIKFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFIGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            1730..4141
                     /locus_tag="VVE42_001329"
     CDS             1730..4141
                     /locus_tag="VVE42_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEU3073516.1"
                     /translation="MIRNKKQDYVLAYKQPASTTYMGWEEEALPIGNGSLGAKVFGLI
                     GAERIQFNEKSLWSGGPLPDSSDYQGGNLQDQYGFLAEIRQALEKRDYNLAKELAEQH
                     LIGPKTSQYGTYLSFGDIHIEFSQQGTTLSQVTDYQRQLNISKALATTSYVYKGTQFE
                     REAFASLPDDLLVQCFTKEGLETLDFTIELSLTCDLASDGKYEQEKSDYKECKLDITD
                     SHILMKGRVKDNDLRFASYLAWETDGDIRVWSDRVQISGASYANLFLAAKTDFAQNPA
                     SNYRKKLDLEQQVIDLVDTAKEKGYTQLKSRHIEDYQALFQRVQLDLEADVDASTTDD
                     LLKNYKPQEGQALEELFFQYGRYLLISSSRDCPDALPANLQGVWNAVDNPPWNSDYHL
                     NVNLQMNYWPAYVTNLLETVFPVINYVDDLRVYGRLAAVKYAGIVSQKGEENGWLVHT
                     QATPFGWTAPGWDYYWGWSPAANAWMMQTVYEAYSFYRDQDYLREKIYPMLRETVRFW
                     NAFLHKDQQAQRWVSSPSYSPEHGPISIGNTYDQSLIWQLFHDFIQAAQELGLDEDLL
                     TEVKEKSDLLNPLQITQSGRIREWYEEEEQYFQNEKVEAQHRHASHLVGLYPGNLFSY
                     KGQEYIEAVRASLNDRGDGGTGWSKANKINLWARLGDGNRAHKLLAEQLKTSTLPNLW
                     CSHPPFQIDGNFGATSGMAEMLLQSHAAYLVPLAALPDAWSTGFVSGLMARGHFEVSM
                     SWEDKKLLQLTILSRSGEDLRVSYPDIEKSVIKMNQEKIKAKCMGKDCISVATAEGDL
                     VQFYF"
     gene            4391..5092
                     /locus_tag="VVE42_001330"
     CDS             4391..5092
                     /locus_tag="VVE42_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3073517.1"
                     /translation="MAYIEMKHCYKRYQVGDTEIVANRDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDALNSDQVLTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGATVIIVTHNGALAPIADRVIHMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            5094..7802
                     /locus_tag="VVE42_001331"
     CDS             5094..7802
                     /locus_tag="VVE42_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU3073518.1"
                     /translation="MIKRKTYWKDLIQSFTGSKGRFLSILILMMLGSLALVGLKVTSP
                     NMEATANAYLTTAQTLDLAVMSNYGLDQADQEELKQTEGAEVEFGYLTDVTMDNGQDA
                     IRLYSKPERISTFQLRKGRLPQSDKEIALATHLQGQYSVGQEISFKEKEEGHSSLKDH
                     TYTITGFVDSAEILSQRDMGYAGSGSGTLTAYGVILPSQFDQKVYNIARLKYQDLAGL
                     NAFSSAYEEKSKQHQEELEQILSDNGKVRLRLLKKEGQESLDKGQETLDKAQTNLQEG
                     KRRLAAAQARIQAQDSQLALFPQAQREQASAQLTQAKQELGKEEDKLKQAEQNLAQEK
                     EKLEKHQQVLDDLAEPRYQVYNRQTMPGGQGYLMYSNASSSIRAVGNIFPVVLYAVAA
                     MVTFTTMTRFVDEERTHAGIFKALGYRSKDIIAKFLLYGLVAGTVGTALGSILGHYLL
                     ASVISSVITKGMVVGETQIQFYWTYSLLALVLSWLASVLPAYLVAWRELHAEAAQLLL
                     PKPPVKGAKILLERIGFIWRRLSFTHKVTARNIFRYKQRMLMTIFGVAGSVALLFAGL
                     GIQSSVAGVPSKQFQQIQQYQMLVSENPSATNQDKVELAEVLKGQEILAYQKIYSKAL
                     YKDFKGKAGLQNITLMMIEKEDLTPFIHLQHHQQELTLKDGIVITAKLAQLAGVKVGQ
                     TLEIEGKELKVAAITENYVGHFIYMSQASYEQLYGQLPQANTYLVSLRDTSATSIESQ
                     AGLLMNQSAVSSVVQNASAIRLFDSVASSLNQTMTILVIVSVLLAIVILYNLTNINVA
                     ERIRELSTTKVLGFHNNEVTLYIYRETIVLSLVGIVLGLVAGFYLHQFLIQMISPATI
                     LFYPQVGWEVYVIPVAAVSIILTLLGFFVNYYLRKVDMLEALKSVE"
     gene            complement(7898..8389)
                     /gene="tpx"
                     /locus_tag="VVE42_001332"
     CDS             complement(7898..8389)
                     /gene="tpx"
                     /locus_tag="VVE42_001332"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEU3073519.1"
                     /translation="MVTFLGNPVSFTGKQLQVGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEGLDNAIM
                     LSDYFDHSFGRDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(8512..9441)
                     /gene="psaA"
                     /locus_tag="VVE42_001333"
     CDS             complement(8512..9441)
                     /gene="psaA"
                     /locus_tag="VVE42_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HEU3073520.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            complement(9473..10321)
                     /locus_tag="VVE42_001334"
     CDS             complement(9473..10321)
                     /locus_tag="VVE42_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEU3073521.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            complement(10318..11040)
                     /locus_tag="VVE42_001335"
     CDS             complement(10318..11040)
                     /locus_tag="VVE42_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEU3073522.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKAKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
BASE COUNT         3288 a         2072 c         2535 g         3263 t
ORIGIN      
        1 agaacatact ttttttaaaa gcaaatatga tacaatttta tttgaaaaaa ataaaaaagg
       61 agattttatt ataaaattaa aaagacttgc tttaattagt ggtatcgtcg gtcttgtggg
      121 aggaatttta cttcttattg gtccttttgt cttgttggga acagcggtaa acacagctgc
      181 tacaactctt aatggaggag ctactgcagg ggctttttca ggtgtagcct tactcttgaa
      241 tgccttgaag attgcaaatc ttgttcttgg tatcattgct attgtttact ataaaggaga
      301 taagcgtgta ggtgcagctc cgtctgtact aatgattgtt tctggtggag ttagtctcat
      361 tccattctta ggctgggttg gggggattct tgctattatc ggaggatctc tattccttgc
      421 aacattgaag aaattcaaat cagaagaata aaaggtattt tagcatgaaa agaacaaaaa
      481 gtttatcggt ataggagtag ctctattatc tctttctctt ctagttgcat gtggaacata
      541 aagttcaaag aatacttcaa caagtaatga tgagaagaca gtagcaacat ccaatagttc
      601 aaaagaaaca atcactttcg atacaccggt tgtaacagac gatgcgattg aatcaatacg
      661 cacttatgca gattatatag atctttataa aaatattttt gatgattatt ttactaaagc
      721 tgaggaaggt ttcaaaggca cagctatgga aaataatgac tcgtttacta aactaaaaga
      781 gtcaactcaa aaattattcg atgcgcagaa aaaaaggtta aataatgaag atagaataga
      841 aacaaccaaa aacaatgtga ttgccaaaca ttgtcaaaca gtcctttcct ttttggtttt
      901 gactagcttt tttgtgaaaa attgtgtaaa atagaataga taaacgaggg gaaacctcgg
      961 aaaatttaaa ggagaatcca tctaatggta aaattggttt ttgctcgcca cggtgagtct
     1021 gaatggaaca aagctaacct tttcactggt tgggctgatg ttgatttgtc tgaaaaaggt
     1081 acacaacaag cgattgacgc tggtaaattg atcaaagaag ctggtatcaa atttgaccaa
     1141 gcttacactt cagtattgaa acgtgctatc aaaacaacta acttggctct tgaagcttct
     1201 gaccaattgt gggttccagt tgaaaaatca tggcgcttga acgaacgtca ctatggtggt
     1261 ttgactggta aaaataaagc tgaagctgct gaacaatttg gtgatgagca agttcacatc
     1321 tggcgtcgtt catacgatgt attgcctcca aatatggacc gtgatgatga gcactcagct
     1381 cacacagacc gtcgttatgc ttcacttgac gactcagtaa tcccagatgc tgaaaacttg
     1441 aaagtgactt tggaacgtgc tcttccattc tgggaagata aaatcgctcc agctcttaaa
     1501 gatggtaaaa acgtattcat aggagctcac ggtaactcaa tccgtgccct tgtaaaacac
     1561 atcaaaggtt tgtcagatga cgagatcatg gacgtggaaa tccctaactt cccaccattg
     1621 gtattcgaat tcgacgaaaa attgaacgtc gtttctgaat actaccttgg aaaataaaaa
     1681 attgtaagcc taggattgat ttctaggctt tttatgttag tatggaagta tgataaggaa
     1741 taaaaaacaa gattatgtac tggcctacaa gcaaccagct tcaaccactt acatgggttg
     1801 ggaagaagaa gctttaccga taggcaatgg ttctttagga gcaaaagtat ttggccttat
     1861 aggggctgaa cggattcaat ttaatgaaaa aagtctctgg tctggaggtc cacttcctga
     1921 tagttcagat tatcagggtg gaaatcttca ggatcagtat ggttttttag ctgagattcg
     1981 gcaggctttg gagaagagag attacaatct ggctaaggaa ctggctgagc agcacctaat
     2041 tgggccaaaa acgagtcaat atgggaccta tctgtctttt ggggatattc acattgagtt
     2101 cagccagcaa ggtacgactt tgtctcaggt gacggactat cagagacagc tgaatattag
     2161 taaggcactt gcgacgactt cttatgtcta taagggaacg caatttgaac gtgaagcttt
     2221 tgcgagtctt ccagatgatc tcttggttca atgttttact aaggaagggt tggaaactct
     2281 agattttact atagaactat ccttgacctg tgatttggct tctgatggaa agtatgagca
     2341 ggaaaaatct gattacaagg agtgtaagtt ggatattact gattctcata tcttgatgaa
     2401 gggaagagtt aaggataatg atctgcggtt tgctagttat ctagcttggg aaacggatgg
     2461 agatattaga gtttggtcag atagggttca gatatcagga gccagttatg ccaatctctt
     2521 cttggccgct aagacggatt ttgcccaaaa tcctgctagc aattatcgca agaaactaga
     2581 tttagagcaa caggtgatag acttggtgga cacagctaaa gaaaagggct atacccaatt
     2641 gaaatcaagg catatcgagg actaccaagc cttattccag cgtgttcaat tggatttgga
     2701 agctgatgtt gacgcatcca ctacagatga tttgttaaaa aattataagc cacaagaagg
     2761 gcaggctttg gaggagctgt tcttccagta tggacggtat ttattgatta gttcgtccag
     2821 agactgccca gatgctctac cagctaacct acagggagtc tggaatgcgg tcgacaatcc
     2881 tccttggaat tcggactatc acttaaatgt caatctgcag atgaattatt ggccagccta
     2941 tgttaccaat cttctagaga cggtctttcc agtcatcaac tatgtagatg atttgcgtgt
     3001 ctatggtcgt ctagcggctg taaagtatgc aggaatcgtc tctcagaaag gtgaggagaa
     3061 tggttggttg gttcatactc aagcgactcc ctttggttgg acggcacctg gttgggatta
     3121 ctattggggt tggtcaccag ctgccaatgc gtggatgatg caaaccgttt atgaagccta
     3181 ttcattttat agggaccaag actatctcag ggagaaaatt tatcccatgt tgagggaaac
     3241 ggttcgtttt tggaatgcct ttttacataa ggatcagcag gcgcagcgtt gggtgtcttc
     3301 tccgtcttat tccccagaac atgggccgat ttcgattggc aatacttatg accaatctct
     3361 gatttggcag ttatttcatg attttattca ggctgctcag gaattgggac tggatgagga
     3421 cttgttgact gaggttaagg agaagtctga tttactaaat cctttgcaaa tcactcaatc
     3481 tggtcgaatc agggagtggt atgaggagga agagcagtat tttcaaaatg agaaagtgga
     3541 ggcccagcat cggcacgctt cccatctagt aggactctat cctggcaatc tctttagcta
     3601 caagggacaa gagtatattg aagcggtacg tgctagcctc aatgatcgtg gagatggcgg
     3661 cacaggctgg tccaaggcta ataagatcaa tctctgggcg cgtttgggag atggcaatcg
     3721 agcccataaa ttattggcag agcagttaaa gacatccacc ttgccaaatc tttggtgtag
     3781 ccatcctcct tttcagatag atggtaattt tggtgctact agtggcatgg cagaaatgtt
     3841 actccagtct catgcagctt atctggtacc tctagctgcc ctacctgatg cttggtcaac
     3901 aggttttgtt tcaggcttaa tggcacgtgg acattttgaa gtgagcatga gctgggaaga
     3961 taaaaaactc ttacagttga ccattttatc aaggagtgga gaagatttgc gagtttctta
     4021 tccagatatt gagaagagtg tgattaaaat gaatcaagaa aaaataaaag cgaaatgcat
     4081 ggggaaagat tgtatttcgg tggcaacagc agaaggtgat cttgttcaat tttattttta
     4141 agaagatgtt ataaggcagt aatttgaaac tgccttttaa taaggattta agaatatagg
     4201 cagtttccaa ctagttgaaa aagcgttata atgataatag gaagtaatac tcaatgaaaa
     4261 tcaaagagca caaacaagga agctagccgc aggttgctca aaacagtgtt ttgaggttgc
     4321 agatggaagc tgacgtggtt tgaagagaga ttttcgagga gtataatttg tttgatagag
     4381 ggtgggtctg atggcttata ttgagatgaa acactgttac aagcgttatc aggttgggga
     4441 cacggagatt gtggccaatc gtgatgtgaa ttttgagatt gaaaaggggg aactggttat
     4501 tatccttggt gcttcaggtg caggcaagtc aacagttctt aaccttcttg ggggaatgga
     4561 taccaatgat gaaggggaaa tctggattga tggtgttaat attgcggatt atagttccca
     4621 ccagcgcacc aattaccgta gaaatgatgt ggggtttgtt tttcagtttt ataatctagt
     4681 ttctaatctg acagctaagg aaaatgtgga actggcttct gaaattgtga cagatgcctt
     4741 gaattctgat caggtcttga cagatgtagg tctggctcat cgtctaaata actttccagc
     4801 ccagctttct ggaggggagc aacagcgagt ctccattgca cgcgcggtag ccaaaaatcc
     4861 taaaattctt ctttgtgatg aacctactgg agccttggat tatcagacgg gcaagcaggt
     4921 tttgaaaatt ctccaagaca tgtctcgtca aaagggagcg acggtgatca tcgtgactca
     4981 taatggagct ttggcgccca ttgctgatcg cgtgattcat atgcacgatg ccagtgtcaa
     5041 ggatgtggtg ctcaaccagc atcctcagga tattgacagt ttggagtact agtatgatca
     5101 agcgaaaaac ttattggaag gacttaattc agtccttcac aggctccaag gggcgttttt
     5161 tatccatctt gatcctgatg atgttgggat ctctagcctt agtaggcctc aaagtaacca
     5221 gtcccaacat ggaggcgaca gctaatgctt atttaacaac tgctcaaacc ttggatttgg
     5281 cagtcatgtc taactatggc ttggatcaag cagaccaaga agaactaaaa cagacggagg
     5341 gcgcagaggt cgagtttggc tatttgacag atgtgactat ggataatggg caggatgcca
     5401 ttcggctgta ctccaaacca gagcgaattt caacctttca gctaagaaag ggacgacttc
     5461 ctcagtcaga caaggaaatc gctttggcca ctcatttgca aggccaatac agcgtgggac
     5521 aggagattag ttttaaagaa aaagaagagg gtcattcctc tttaaaagac catacttata
     5581 ccattactgg ttttgtggat tcggctgaaa tcctctccca gcgagatatg ggctacgcag
     5641 gaagtggaag tgggactctg acagcctatg gggtgatttt acctagtcaa tttgatcaga
     5701 aagtctacaa tatagctcgt ttgaaatatc aagatttagc aggtttaaat gccttttcat
     5761 cagcttatga agaaaaatcc aagcaacatc aggaagagct tgaacaaatt ttatcagata
     5821 atggcaaggt acgtctgcga cttttgaaaa aagaaggaca agagtctcta gacaaggggc
     5881 aagagaccct tgacaaggct cagactaatt tgcaggaagg caagcgtcgt ttagcagctg
     5941 ctcaagctcg tatacaggct caagacagtc aactagcctt gtttcctcaa gctcagagag
     6001 agcaggctag tgctcaactt acccaagcca agcaggaatt gggcaaggaa gaggacaaac
     6061 taaagcaagc tgaacaaaat ctagcccaag aaaaggaaaa attagaaaaa catcagcaag
     6121 tcttggatga tttggcggag ccaaggtatc aggtttataa tcgtcagacc atgccaggtg
     6181 gtcagggcta tcttatgtat agcaatgctt catccagtat tcgagcagtg ggcaatatct
     6241 ttcctgtggt actttatgcc gtagcagcca tggtgacctt tacgaccatg actcgctttg
     6301 tagacgaaga gcgaactcat gcagggattt ttaaggcctt gggttaccgt agtaaggata
     6361 ttatcgccaa gtttctcctt tatggactag tagctgggac tgtcggaacg gctctaggta
     6421 gtatacttgg tcattatttg ctagccagtg taatttcaag tgtcattaca aaaggcatgg
     6481 tggtgggaga aacccagatt cagttctatt ggacctatag cttactagct cttgtcttga
     6541 gctggttggc gagtgtgtta ccagcctatc tggtggcttg gagggaactt catgccgaag
     6601 cagcccagct tctacttcct aaacctcctg tcaaaggagc taaaatctta ttggagcgta
     6661 tcggttttat ctggcgtcgt ctcagtttta ctcataaggt aacagcccgc aacatctttc
     6721 gttataagca gagaatgttg atgacaatct ttggtgtggc aggttctgta gctctgctct
     6781 ttgcaggttt gggaatccaa tcttctgtag caggagttcc gtctaaacag tttcaacaaa
     6841 tccaacagta tcagatgctt gtctctgaaa atcctagtgc gaccaatcag gacaaggtag
     6901 agctagcaga agtgttgaaa gggcaggaga tactagccta ccagaaaatc tattctaaag
     6961 cgctatacaa ggatttcaaa ggcaaagctg gtcttcaaaa cattactctt atgatgatag
     7021 agaaggaaga tttgactccc tttatccatc ttcaacatca tcagcaggag ctgacattaa
     7081 aagatggcat cgttattaca gctaaactcg cccagctggc aggtgtcaag gttgggcaga
     7141 ctttagaaat tgaaggtaag gaactaaagg tcgctgctat tactgagaac tacgttggtc
     7201 actttattta tatgagtcag gctagctatg agcaacttta cggacagcta ccccaagcca
     7261 acacttatct ggtctcatta agggatacca gtgcaactag tatcgaaagt caggcgggct
     7321 tgcttatgaa tcaatctgcg gtgtccagcg ttgtccaaaa tgcttcagcc attcgactct
     7381 tcgactctgt cgctagctca ctcaatcaga ccatgaccat cttggtcatc gtatcggttc
     7441 tattagctat tgtcatcctt tacaatctga ccaatatcaa cgtagctgag agaatccgtg
     7501 aactctccac taccaaggtt cttggttttc ataataatga agtcaccctc tacatttacc
     7561 gtgagacgat tgtgctatcc cttgtgggaa tcgtacttgg tctggtagct ggtttctatt
     7621 tacaccaatt tttgattcaa atgatttcgc ctgcgactat tctcttttat ccgcaggtag
     7681 gctgggaagt ctatgtaatc ccagtggcag cagtaagcat cattttgacc ttgcttggtt
     7741 tcttcgtcaa ttattatctg agaaaggttg atatgttaga agccctgaaa tctgtagagt
     7801 aaggtagtta tttttagctg attgaacttc tatttactaa tattcaaaaa tcctctgtca
     7861 caaagagcag ggagctcttt gtgacagagg attttttcta tagggcttta gcagctgcaa
     7921 ttgcggcttc gaagtttggc tcagaattga tattatccac gtattcaacg tagcgaatcg
     7981 tattgtcagt atcgaggaca aagactgcgc gtgctaatag gtgccattcg ttgatcaaga
     8041 gggcataatc gcgcccgaaa gaatggtcaa agtagtctga aagcataatg gcattgtcaa
     8101 ggccttcagc accgcaccaa cgtttttgag caaaaggtag gtccattgaa acagtcaata
     8161 ctaccgtgtt gtccagtcca gccaattctt cattaaaacg acgtgtttga gttgagcaga
     8221 tgcctgtatc gatagaagga acgacactca agactttttt cttgccatca aaatcagcca
     8281 gagatttttt agaaagatct gttgtagtaa gagaaaaatc aagcgccttg tcgccgactt
     8341 gtagttgttt acctgtaaag ctcacaggat ttccgagaaa agttaccata ggatactcca
     8401 atcttttttc ttccattgta tctgaaacag tcagaatttt ccaatgattt gaccggaaat
     8461 gtgtgcatag aaaaaacgcc agctcacatg agaatgacgt ttttcagagg cttattttgc
     8521 caatccttca gcaatcttgt caaggttgta tttcatcatg ctgtagtagc tgtcgccttc
     8581 tttaccttgt tctgcgatag agtcagtaaa gatttgtgcg tagattggga tgtttgtgtc
     8641 ttgagaaaca gttttcattg gacggtcatc cacacttgat tctacaaaga gtgatggaac
     8701 ttttgtttgg cgaagttttt caaccaaggt cttgatttgt tcaggagttc cttcttcttc
     8761 agtattgatt tcccagatgt aggcacttgg aacaccatag gctttagaga agtatttgaa
     8821 tgctccttcg ctggttacaa tgagtttctt ttcagcaggg atcttattaa atttatcctt
     8881 actttcttta tcaagtttgt ctaacttatc agtatattct ttgagatttt tttcatagaa
     8941 ttctttattg ttagggtctt tggcgctcaa ttgtttagcg atatttttag caaaaataat
     9001 accgttttca aggttaagcc aagcgtgtgg gtcttctttt cctttttcat tttgaccttc
     9061 aaggtagata acatcaacgc cgtcgctgac tgcgaagtag tctttgtttt cagttttctt
     9121 ggcattttct accaattttg taaaccaagc attgccacct gtttcaaggt tgataccgtt
     9181 atagaaaatc aaatcagcct cagaagtttt cttaacgtct tcaggaagtg gttcgtattc
     9241 gtgtgggtct tgcccaatcg gaacgatact atgaaggtca attttgtcac cagcaatatt
     9301 tttagtaata tcagcgatga ttgagtttgt agcaacaact tttagttttt gaccagaagt
     9361 tgtatctttt tttccgctag cacatgctac aagaatgatt gcagaaagaa agagaacgag
     9421 taatgtacct aattttttca ttagatcctc caatttatta gggctttgcc ccttatttta
     9481 acaaatgttt atttttcagt ttcaaatatc gttgtttggg agcgataaag aagctaatga
     9541 gaaagaaact agcagctgta agcacgatac tagaacctgc cgcaacatta aaactatagc
     9601 caataaagag tcccaaaact gaagcagtag ctccgaaggt tgaggaaagg aaaatcatac
     9661 ttttcagact attagcatac agataagcag ttgcagctgg ggtaattagc atggctacaa
     9721 tcaggatagt tccgacactt tgcatggctg tcacagacac gagagtcagg agtaccatga
     9781 gaaggtagtg atagaaattg acaggcattc ccatggcttt agccaagagt tcatcaaagg
     9841 aagttatcaa gagttgcttg aagaaaatcc agattaacaa gaggatggct gcccccacac
     9901 ccatagtaat aaacatatcc gtatcttgga cggccaggat attaccaaaa aggatatgga
     9961 aaaggtcagt tgaactttta gcgacaccaa tcaagatgat accgagagct aagaaagaag
    10021 aaaaggtaat gccgatggcg gtatcgcttt tgataatcga gtttcctttg atgtaggtaa
    10081 tgatgatggc agctagcaat ccaaagacaa tggctccgat aaagaagtca aggcccaaga
    10141 tgaaggagag ggctacacct ggtaagacag catgtgaaat ggcatctccc atgagtgaca
    10201 tcccgcgtag aataatgaaa catcccacag ctccagctac aatcccgacg acaatagctg
    10261 ttatcaaggc attttgtagg aaatggaatt tttgcaatcc atcgataaat tctgcaatca
    10321 taggtcacct ccattgaaaa agagttgatt accgtaagct tcttttagat tggtttcggt
    10381 aaaagtttct tttgttggac caaaggcaat cacttctcga ttgacaagta agacttgatc
    10441 gaagtagtgg ggaatcttgc tgaggtcgtg gtgaacgatg agaaccgtct tcccagcttt
    10501 tttcaaatct ctcagcgtat tcatgatgat ttcctcactg acagagtcaa tcccagcaaa
    10561 gggttcatcc aagaggatat agtcggcttc ctgcaccaaa catctggcaa tcaagacccg
    10621 ctggaattga cctccagaca gttgactaat ttgacgttca gcgtagtcag ctaggccgac
    10681 gatttcaagg gcctcttgca ctttcttcca atgtttagcc tttaaacttc gaaagagagg
    10741 aatagaggga aatagtccta acgagacgca ttccttgacc ttgatgggaa agttgtagtc
    10801 gatattgatt ttttgttcga cataggcaat tcggtgtaag gattttttaa cttccttgtc
    10861 atcgagaaat gcctgacctt gatgtgggat aattcccagc atacctttta atagtgttga
    10921 tttcccagcg ccgtttggac caatgatgcc ggtaattgtt ggtccatgga gcactagtga
    10981 aatatcctta agtgccaacg tttctttgta ggagacactg aggttttcga tacgtatcat
    11041 aaacttgtat tcctcctgtc tcttaatata cattaaaaaa aaaattaagt caagttaatt
    11101 tttgaaaaaa ttaaaataat aactgaaaaa tagattctaa agataacttt caggataa
//
LOCUS       DAVGJL010000065        10985 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.065, whole genome shotgun sequence.
ACCESSION   DAVGJL010000065 DAVGJL010000000
VERSION     DAVGJL010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10985
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.065"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(<1..1887)
                     /locus_tag="VVE42_001336"
     CDS             complement(<1..1887)
                     /locus_tag="VVE42_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEU3073523.1"
                     /translation="MKSFLKTYRTYFISFIIPVVIMSGVYLSQGIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSSLFYTFTSGLGLNFYALSSYYLGSFLAPLVYFFDLTNMP
                     DAVYLTTLLKFGLIGLSTFFSLNKLFQSIPQTLKLALSTSYALMSFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTRKSSVLDFIVISFLAGMASLIMTLPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLLPFILTILFFTLKSIKFHVKLIYIIFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLKRLKELKVWNFLVSLFLVVTGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSAYSRDIPAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVNFDY
                     FSPIP"
     gene            complement(1949..3571)
                     /locus_tag="VVE42_001337"
     CDS             complement(1949..3571)
                     /locus_tag="VVE42_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU3073524.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEVQAKVKELWKD"
     gene            3777..4802
                     /gene="trpS"
                     /locus_tag="VVE42_001338"
     CDS             3777..4802
                     /gene="trpS"
                     /locus_tag="VVE42_001338"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEU3073525.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
     gene            4954..6432
                     /gene="guaB"
                     /locus_tag="VVE42_001339"
     CDS             4954..6432
                     /gene="guaB"
                     /locus_tag="VVE42_001339"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEU3073526.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SENLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(6483..7580)
                     /gene="recF"
                     /locus_tag="VVE42_001340"
     CDS             complement(6483..7580)
                     /gene="recF"
                     /locus_tag="VVE42_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEU3073527.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSDQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNITDKQNLSESFKIALEKSKSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKAAVNGN"
     gene            complement(7583..7951)
                     /gene="yaaA"
                     /locus_tag="VVE42_001341"
     CDS             complement(7583..7951)
                     /gene="yaaA"
                     /locus_tag="VVE42_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEU3073528.1"
                     /translation="MEYKLFEEFITLQALLKELGITHSGGAIKSFLSEHSVYFNRELE
                     SRRGKKLRIGDKVDIPDMNIDILLTQPTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQAPRFPGR"
     gene            8110..9360
                     /locus_tag="VVE42_001342"
     CDS             8110..9360
                     /locus_tag="VVE42_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEU3073529.1"
                     /translation="MELVHGISTHFIQSKKFKTNKITVRFTAPLSLDTIAGHMLSASM
                     LETANQMYPTSQDLRRHLASLYGTDMSTNCFRRGQSHIIELTFTYVRDEFLSRKNVLT
                     SQILELVKETLFSPVVVDNGFDPALFEIEKKQLLASLAADMDDSFYFAHKELDKLFFH
                     DERLQLEYSDLRNRILAETPQSSYSCFQEFLANDRIDFFFLGDFNEVEIQNVLESFGF
                     KGRKGDVKVQYCQPYSNILQEGMVRKNVGQSILELGYHYRSKYGDEQHLPMIVMNGLL
                     GGFAHSKLFTNVRENAGLAYTISSELDLFSGFLRMYAGINRENRNQARKMMNNQLLDL
                     KKGYFTEFELNQTKEMIRWSLLLSQDNQSSLIERAYQNALFGKSSADFKSWIVKLEQI
                     DKDAICRVANNVKLQAIYFMEGIE"
     gene            9357..10640
                     /locus_tag="VVE42_001343"
     CDS             9357..10640
                     /locus_tag="VVE42_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEU3073530.1"
                     /translation="MTKVVFEEKYYPAVKEKVYRTRLANGLTVALLPKKEFKEVYGSV
                     TVQFGSIDTLVTEVDGDVKQYPGGIAHFLEHKLFEREDSSDLMSAFTSLGADSNAFTS
                     FTKTNYLFSATDYFLENLYLLDELVTSAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKPTDSMRMEVSSPKLAIGVRGKREVSEADCY
                     RHHILLKLLFAMMFGWTSDRFQKCYESGKIDASLSLEVEVTSRFHFVMLTMDTKEPVA
                     LSHQFRKAIRNFTKDLDITEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGETIF
                     DLPKILQEITLEDVLDAGHHLIDDGDIVDFTIFPS"
     gene            10682..>10985
                     /locus_tag="VVE42_001344"
     CDS             10682..>10985
                     /locus_tag="VVE42_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU3073531.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKK"
BASE COUNT         3518 a         1731 c         2202 g         3534 t
ORIGIN      
        1 aggtattgga gaaaaataat caaaattgac gttagcaagt tgatttaaaa atgaggcctg
       61 attatccaag gtatgttcat tgaacttgac atcattgtaa acagattgac tagcaactgc
      121 aatcggaaga gagtattgat tttcatatag ggtaagatta tctttttgat agatatcttt
      181 aaagccatac ttatcaatag gactgtctga gatattgtac tggataccaa ataaactatc
      241 agccaaaata ctattatttg catatcggag attgagatta gtcccagagg atttaaaacc
      301 aagtttatct aaagtagagc ttgatgaacg atttcgaaca gatgaaaatt gagagattcc
      361 attgtagttg aatttcatac tgtcatttcc tgtctgagtt tgtagttttt cagtacgagt
      421 aaattgattt ccaatatatg ttgagaaaga ttccatagct gggatatctc gactataagc
      481 acttcgagaa gcaaatcccc attccttagc aattccgtcc atttgagatg aagcatttaa
      541 actcatttca accagtataa ataaagagat tagaatggca aatagattca cagatataaa
      601 ctttttgata actgcaagga gtaaaagaga atagacaacc aaaaattcaa gagtaagcag
      661 aatattcaaa tctgttaaaa aagaataatg cgattttaga tagatggtag ctaaaaatcc
      721 tgttactaca agaaaaagcg aaactaaaaa attccagact ttaagttctt tcagacgctt
      781 taagacttct gctgctgtgt aaattaacaa ggtagagaaa atccaagcat agcgatgtaa
      841 aaacatgttt ggagtatgca tgccttgcca aaataagtca agagcttcta tgtaaaagct
      901 tgcaattaga aatgcaaaga atattatata tatgagtttc acgtgaaact taatagattt
      961 cagcgtaaaa aataaaatgg tcaaaataaa gggaagtagt ccaacaaaaa tcattgggat
     1021 ggccccatac tttgttgtgt caaaggaacc aatgaattgc ttagcaaaga gatcaagata
     1081 ccagctactt tcagtttgaa actttgtaac ttcagtcaat ttttccccat gtgtctgtaa
     1141 atcaaacaga gtgggaagag tcataatcaa actagccata ccagctaaaa aggagataac
     1201 tatgaaatca agaacagatg attttcgagt cttaaagtcc cacgaaattt gacagagata
     1261 ccagaaaata agaaacaata ctgtcatata tccaaaataa taattttgaa taaataagat
     1321 tgacagactt gtaaagtaca ataggagttt cttttcagtt atcagtagat gtaaaccagt
     1381 tataattaaa ggaatcaaga taaaaacatc tagccaggtt tttatctcta attgactgac
     1441 agtgaaactc atcagagcat aggaagtaga taaggctagt tttaaagtct gagggataga
     1501 ttgaaacaat ttattcaaac taaaaaaggt tgacagacca atcaatccaa attttaagag
     1561 agtggtcaga tagacagcat ctggcatatt cgttagatca aaaaagtaaa ctagaggcgc
     1621 gagaaaacta cccaagtaat aactagatag ggcatagaag tttagcccta gaccacttgt
     1681 aaaggtgtaa aacagactac tatttccatg taggatattt cgtaaggcta catcaaaaat
     1741 aacgtattga tgaaagccat ctcctaatag aggagagttg tcgctattcc agtagatacc
     1801 ttgagataga tatactccag acataatcac tacaggaatg atgaaagaaa taaaataggt
     1861 tcgatatgtt tttaaaaatg atttcatgtt acctcgtaga atgatagaaa actcagttgg
     1921 ttaacccaac tgagttttga agttttattt agtctttcca aagttcttta acttttgctt
     1981 gtacttctgc attttctagg aattcatcgt aggtttcatc gatacggtca atgacgccat
     2041 ttttagataa gacaatgata tggttagcca aagtttgaat aaattcgtgg tcatggctgg
     2101 caaagatgat tgattcttta aagtttttca atccatcatt caagcttgag atagattcca
     2161 agtccaagtg atttgttgga tcatcaagta caaggacatt tgattttaag agcatgagtt
     2221 ttgaaagcat gacacgaact ttttctcccc ctgacaagac atttacaggt ttgttaactt
     2281 catctccaga gaagagcata cggccgagga agccacgtag gaaagtattg tcatcttctt
     2341 ctttacttgc gaattgacgc aaccagtcaa gaattgattc tcctcctgca aaatcagctg
     2401 agttatcttt tggtaggtaa gattgactag ttgtaactcc ccacttgaca gttccttcat
     2461 agtcaatatc tcccatgatt gcacgaatta atgcagtcgt ttgaatatca ttttgtccaa
     2521 taagtgctgt cttatcatct ggacgcaaga tgaaactaat attatccaag atagtttcac
     2581 catcaatctt tacagttaga ttttctactg tcaagagatc attaccaatc tcacgttccg
     2641 ctttaaagtt gataaatgga tatttacgac tagatggtac aatctcttct agctcaatct
     2701 tatcaagcat tttcttacgt gatgttgcct gccttgactt agaagcattg gcagagaaac
     2761 gagcaacgaa ttcttgcaat tgtttaattt tttcttctgc tttagcatta cggtctgcta
     2821 gcaatttagc agcaagctca gaagattcct tccagaagtc gtagtttccg acatagagtt
     2881 tgatttttcc aaagtcaagg tcggccatgt gagtacaaac tttgtttaag aagtgacggt
     2941 cgtgggatac tacgataact gtgttatcaa agtcaatcaa gaagtcttct aaccaagtaa
     3001 tcgattggat atccaaaccg ttagtaggct cgtccaagag aagaacatct ggtttaccaa
     3061 aaagtgcttt ggcgaggaga acctttactt tttcaccgtt ggccaattcg ctcatgtttt
     3121 ggtagtgtaa ttcttctgga atgtttaggt tttgaagtag ttgagaggct tcactctctg
     3181 cttcccaacc tccaagttcg gcaaactctc cttcgagttc ggcagcacga accccgtcct
     3241 cgtctgagaa atcttccttc atgtagatag catctttctc tttcatgatg ctataaagtt
     3301 tttcatttcc catgataacg acatcaatgg cacgttcatc ttcgtagtca aagtgatttt
     3361 gacgaagaac agagagacgt tcatctggac caagagagat gtgaccagta gtaggttcga
     3421 tatctccagc taaaattttt aaaaaggttg attttccggc accattagca ccgattaatc
     3481 cgtaagtatt tccttctgta aatttgatat tgacatcatc aaaaagtttg cgatcactaa
     3541 aacgtagtga aacatcagat actgtaagca atatttttct cctatatgtg taatatattt
     3601 attctactag aaaatacaga aatattcaaa tttttatttg tcaattttgt gtaaattata
     3661 tttacagtat cctttacaca aatctgtaaa aagcaaggct gatttatttt gataaattac
     3721 ggttatttca ttaaaaaaat gctataattg aaaggactat atcgaaggag aacaaaatga
     3781 ctaaacccat tattttaaca ggagaccgtc caacaggaaa attgcatatt ggacattatg
     3841 ttggaagtct caaaaatcga gtattattac aggaagagga taagtatgat atgtttgtgt
     3901 tcttggctga ccaacaagct ttgacagatc atgccaaaga tcctcaaacc attgtagagt
     3961 ctatcggaaa tgtggctttg gattatcttg cagttggatt ggatccaaat aagtcaacta
     4021 tttttattca aagccagatt ccagagttgg ctgagttgtc tatgtattat atgaatctag
     4081 tttcgttagc acgtttggag cgaaatccaa cagtcaagac agagatttct cagaaaggat
     4141 ttggagaaag cattccgaca ggattcttgg tctatccaat cgctcaagca gctgatatca
     4201 cagctttcaa ggctaattat gttcctgttg ggacagatca gaaaccaatg attgagcaaa
     4261 ctcgtgaaat tgttcgttct tttaacaatg catataactg tgatgtcttg gtagagccgg
     4321 aaggtattta tccagaaaat gagagagcag ggcgtttgcc tggtttagat ggaaatgcta
     4381 aaatgtctaa atcactaaat aatggtattt atttagctga tgatgcggat actttgcgta
     4441 aaaaagtaat gagtatgtat acagatccag atcatatccg cgttgaggat ccaggtaaga
     4501 ttgagggaaa tatggttttc cattatctag atgtttttgg tcgtccagaa gatgctcaag
     4561 aaattgctga tatgaaagaa cgttatcaac gaggtggtct tggtgatgtg aagaccaagc
     4621 gttatctact tgaaatatta gaacgtgaac tgggtcctat tcgtgagcgc cgtattgaat
     4681 ttgctaagga tatgggagaa gtttataata tgattcaaaa aggtagtgaa agagcgcgtg
     4741 aagttgcggg tcaaacccta tctgaggtaa aaggggcaat gggacttcat tactttaact
     4801 aagtttattt tacaagaaac tatcatttct atctcgaaga aaagatagtt ttttatttac
     4861 ccctgtaaca tttgacaaat ttgtatagaa tggtatgata gatacaatat aaaaagagtc
     4921 aagctcaaaa agaaagaaaa gaggaaactt cgaatgtcta attgggacac taaatttttg
     4981 aaaaaaggtt ttacctttga tgatgtattg cttattccag ctgaaagtca tgtgttgcct
     5041 aacgatgcag atttaacaac taaattggca gataatttga ctttaaatat cccaattatt
     5101 accgctgcca tggacacagt tacagagagt caaatggcca ttgctattgc tcgtgcaggt
     5161 ggtctcggag ttatccataa aaacatgtca attgctcaac aagcagacga ggttcgtaag
     5221 gtaaaacgtt ctgaaaatgg agttattatt gatccgttct tcttgacgcc tgaacataca
     5281 attgctgaag cagatgagct tatgggtcgt taccgcatca gtggtgttcc agttgttgaa
     5341 acacttgaaa atcgtaaatt ggttggtatt ttgacaaacc gagatcttcg ttttatttca
     5401 gattataatc aaccaatttc aaaccatatg actagtgaaa atcttgttac tgctcctgtg
     5461 ggtacggatc ttgcaacggc tgagagtatt cttcaagagc atcgtattga aaaacttccg
     5521 ttggtcgatg aagaaggcag tctttctggt ttgatcacta tcaaagatat tgaaaaagtt
     5581 attgagtttc caaatgcggc taaagatgag tttggtcgtc ttctagttgc aggtgcagta
     5641 ggtgttactt cagatacatt tgaacgtgca gaggctcttt ttgaggcagg agcggatgcg
     5701 attgttattg atactgcaca tggtcattct gcaggtgtct tgcgtaaaat tgccgagatt
     5761 cgtgctcatt tcccagatcg gactttgatt gctggaaata ttgctactgc tgaaggtgca
     5821 cgtgcccttt atgaagcggg tgtagacgtt gttaaggttg gtattggacc aggttctatc
     5881 tgtactactc gtgtgattgc tggtgttggt gttccgcaag taacagctat ctacgatgct
     5941 gcagctgttg cgcgcgaata tggtaaaacg attattgctg acggtgggat caagtattct
     6001 ggagatattg taaaagcact tgctgcaggt ggaaatgctg ttatgcttgg atctatgttt
     6061 gctggaactg atgaagctcc aggcgaaact gaaatcttcc aaggacgtaa attcaagact
     6121 taccgtggta tgggatcaat tgctgctatg aagaaaggtt caagcgaccg ttatttccaa
     6181 ggttctgtca atgaagcaaa caagcttgtt ccagaaggaa ttgaaggtcg tgttgcttat
     6241 aaaggagcgg cagctgatat tgttttccaa atgattggtg gtattcgctc tggtatgggt
     6301 tactgtggtg cagctaacct taaagaacta cacgataatg ctcaatttat tgaaatgtct
     6361 ggtgctggtt tgaaagaaag ccatcctcat gatgtgcaaa ttactaatga ggcaccaaat
     6421 tattctatgt aaaaaacaat gaaaagaact ccagtgaaaa caggagttct tttacaatgt
     6481 tgtcaatttc catttacagc agctttacca tcctgaatag tgaagatact tagattttct
     6541 ggcagatttt gaagatggtc taagcttgtt gttgtgataa aggtttggat tgattgagaa
     6601 atcgtttcta ataattttaa ctgtctagtg ttgtcaagtt cactcatcac atcgtcaagc
     6661 agtaatatcg gagattctgt ggtaatgctt tccattaatt cgatttctgc taattttatc
     6721 gagaggacga gactacgatg ttgaccttgg cttccgaaac tagcatccat cccatttata
     6781 taaaaagaaa tgtcatctcg atgaggaccg acaccagtat tctttttaaa taaatctctg
     6841 gatttacttt tttctaaagc aattttgaaa gattcggata agttttgttt gtcagttata
     6901 ttgacagaag attgatagga tattgacaac tcttcgatct gattagagag ttcaaaatgt
     6961 ttcttacgcc caaatgattc tagttttttt atgaaatcta agcggtgatt cattacacga
     7021 catccataat caactagctg atcatctaac acagaaagga atgtttcatc tattttttga
     7081 tctgatttta ggtaagtgtt tctttgcttt aggatgtggt tataattggt taagtcagat
     7141 aagtagattg gcttaatttg cccaagttcc atatcaatga attttcgtcg aatcgaaggt
     7201 gctcctttaa ttagttgtaa atcttcagga gcaaataaga caacattcat gtgtcctaca
     7261 taatctgaaa ggcgtgcctg ttttaagtga ttaacttttg ttacacggcc tttttgtgtt
     7321 agttcgattt ctaggggaat ggatccagtt tttttctgaa cgagacctga aagatgaagt
     7381 tgttcctcat caaaatgaat gagattttta tctgttcgag ttcgatgact acgcgttaag
     7441 gctaaaaaat agatagcctc taacatgttt gtttttcctt gtgcattacg tcccaaaaag
     7501 acattcaatt taggattaaa gtctattttc gtttctttgt aattacgaaa agtcttgaga
     7561 gatagatgtt gtagccacat gattatctac cagggaatcg tggagcttgt tgacttttag
     7621 gtgatgaagc aggtttcgat ttgtcttttt taactccctt attcatcttt ttgacaagtt
     7681 tagcgatccg ttctttttca actttatcag cttggtattc atcttgttct tcagaagtag
     7741 gttgtgtcaa caagatgtca atgttcatgt cagggatgtc aactttatcg ccaatacgaa
     7801 gtttttttcc acgacgactc tctaattccc tattaaagta aacagaatgt tcagagagaa
     7861 atgatttaat agctcctccg ctatgtgtaa ttccaagttc tttgagtagt gcttggaggg
     7921 taataaattc ttcaaataat ttgtattcca taattcacct caatctttgg tattatacca
     7981 tattttccta aaaatagtga gagagacagc gagaaatgta agcgattttt atttcaaaag
     8041 tgttacaggg aacaagaaaa tttcaggttt tcgtgatata atagaagtct gtatataagg
     8101 aggtaaatca tggagttagt gcatggaatt tcaacacatt ttatccaatc aaaaaagttt
     8161 aaaacaaaca aaattaccgt gcgttttacc gctccattat cccttgatac gattgcaggt
     8221 cacatgttga gtgcaagtat gctagagact gctaatcaga tgtaccccac ttctcaagat
     8281 ttgaggagac acttggccag tctatacggt acagatatgt caaccaattg tttcagaaga
     8341 gggcaaagcc acattataga attgacattt acctatgttc gtgatgagtt tttaagtagg
     8401 aaaaacgtgc taacctctca gattttggaa cttgtaaaag aaactctttt ttcacccgta
     8461 gtagttgata atgggtttga tccggcctta tttgaaattg agaaaaaaca attgctagca
     8521 agtttagcag ctgatatgga tgattctttt tattttgcac ataaagaatt ggataaattg
     8581 ttttttcatg atgaacgtct tcaattggaa tatagtgatt tacgaaatcg tattttagct
     8641 gaaactccac aaagttctta ttcttgtttc caagaatttt tagccaatga tcgaatagat
     8701 ttctttttcc taggtgattt taatgaggtt gaaattcaaa atgtattaga atcatttggc
     8761 tttaaaggtc gaaaaggaga tgtgaaggtt cagtattgtc aaccttattc taatatcctt
     8821 caggaaggta tggttcggaa aaatgtggga caatccattt tggaattagg ttatcattac
     8881 cgttctaaat atggtgatga gcaacattta cccatgattg taatgaatgg tttacttggt
     8941 ggatttgctc attctaagct ctttacaaat gtccgtgaaa atgctggatt agcttatacc
     9001 atttcaagtg agcttgattt atttagtgga ttcttgagga tgtatgctgg tatcaatcga
     9061 gaaaatcgta accaggctcg taaaatgatg aataatcaac tgcttgattt aaaaaaaggt
     9121 tattttacag agtttgagtt aaatcagacc aaggaaatga ttcgttggtc gttgttactt
     9181 tctcaagata atcaatcttc attgattgaa cgtgcttatc aaaatgcctt atttggaaaa
     9241 tcttcagcag actttaaaag ttggattgta aagcttgaac agattgacaa agatgctatt
     9301 tgtagagtag ctaataatgt gaaactacaa gcgatttact ttatggaagg aatagaatga
     9361 caaaggttgt ttttgaagaa aaatactatc cagctgtaaa agaaaaggtt tatcgaactc
     9421 gtttggccaa tggattgaca gttgctcttt tgcctaaaaa ggaatttaaa gaggtttacg
     9481 ggagtgtaac tgtacagttt ggttcgatag atacgcttgt cacagaagtt gacggagatg
     9541 taaaacaata tcctggagga attgctcatt ttcttgaaca taaattattt gagagagaag
     9601 attctagtga tttgatgtcg gcttttacga gtctaggtgc agatagtaat gcctttacaa
     9661 gctttacaaa aacaaactat cttttttcag caacggatta ttttttagaa aatttatatt
     9721 tacttgatga attggtaaca tcagcacact ttactgaagc ttccattctg acagagcagg
     9781 atattattca gcaagaacga gaaatgtacc aagatgatcc agattcgtgt ttattctttt
     9841 caactttagc gaatttgtat cctggtacac ctttagcaac tgatatagtt ggaagtgagg
     9901 agtccatttc ccaaatcaat ctaactaatt tgcaagaaaa ttttacaaag ttttacaaac
     9961 ctgtaaacat gtctctgttt ttagttggta attttgatgt ggagcgagta caggactatt
    10021 ttgaaagcaa agaactgaaa gattcagatt ttcaggaagt agcaagagaa aagttgtttt
    10081 tacagcctgt aaagccaaca gatagtatga gaatggaagt atcttctccc aaactagcga
    10141 ttggagttag aggtaagcga gaagtttctg aagcggattg ctatcgacat catattttat
    10201 taaaattatt gtttgcaatg atgtttggtt ggacttcgga tcgttttcaa aaatgttatg
    10261 aatcaggtaa aattgatgca tccttatctc tggaagttga agtaacaagt cgctttcatt
    10321 ttgtcatgtt gacaatggat acgaaagagc cagttgcttt gtctcatcaa tttaggaagg
    10381 ctattcgtaa ttttacaaag gatttagata ttacagagga acatttagat attatcaaaa
    10441 gagagatgtt tggcgaattt ttcagtagca tgaactctct tgaatttatt gcaacgcaat
    10501 atgatgcttt tgaaaatggt gagacaattt ttgatttgcc gaaaatttta caggaaatta
    10561 ctttagagga tgtccttgat gctggacatc atttaataga tgatggtgac atagttgatt
    10621 ttacaatatt cccatcgtag taacctatta taatagacac tagaaagaag ggatgacaag
    10681 tatgagaaaa aaaacaattg gagaggtttt acgattagct agaatcaatc agggattgag
    10741 tttagatgaa ttgcagaaaa agacagaaat ccagttagat atgttggaag caatggaagc
    10801 agacgatttc gatcaacttc caagtccttt ttacacgcgt tctttcttga aaaaatatgc
    10861 atgggctgtt gagttagatg accaaattgt tttggatgct tatgattctg ggagtatgat
    10921 tacttatgag gaagtagatg ttgatgaaga tgagttgaca ggtcgtagac gttcaagtaa
    10981 gaaaa
//
LOCUS       DAVGJL010000066        10916 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.066, whole genome shotgun sequence.
ACCESSION   DAVGJL010000066 DAVGJL010000000
VERSION     DAVGJL010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10916
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.066"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            19..204
                     /gene="briC"
                     /locus_tag="VVE42_001345"
     CDS             19..204
                     /gene="briC"
                     /locus_tag="VVE42_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
                     /protein_id="HEU3073532.1"
                     /translation="MTGTNTFTVLSTEDLEQTSGGLAVWEDGYSRWLYYREFAPYMRQ
                     GALNSYIDAWKYGFRAG"
     gene            222..944
                     /locus_tag="VVE42_001346"
     CDS             222..944
                     /locus_tag="VVE42_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU3073533.1"
                     /translation="MRNTKKLRQFGIFLLIILLSTYLPQTIGLYVTIILGLGADVYSL
                     ILTMGLVGSFLLLIWRLKKKKMLFIFEKKSWNWSFVFYLFATYVVYQILGNFWARYAH
                     LINHRNIHDEYFTVLFSNGQPTFLSTILSFVLPVIIGPVFEETLDRGYFMNTFFPKSK
                     YYLDVILSGLIFGLSHLILSHRDPISLLYYSLIGFFFALVYRSTDNLRLTILCHSFFN
                     FLNHAKPIWIFVYNYIYYHFFR"
     gene            complement(994..1416)
                     /gene="nusB"
                     /locus_tag="VVE42_001347"
     CDS             complement(994..1416)
                     /gene="nusB"
                     /locus_tag="VVE42_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEU3073534.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTDVETACRFAYTHDREDT
                     DVQLPAFLIDLVSGVQAKKEELDKQITQHLKAGWTIERLTLVERNLLRLGVFEITSFD
                     TPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            complement(1409..1798)
                     /locus_tag="VVE42_001348"
     CDS             complement(1409..1798)
                     /locus_tag="VVE42_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU3073535.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGRGIYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            complement(1820..2380)
                     /gene="efp"
                     /locus_tag="VVE42_001349"
     CDS             complement(1820..2380)
                     /gene="efp"
                     /locus_tag="VVE42_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEU3073536.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            complement(2509..3951)
                     /gene="gatB"
                     /locus_tag="VVE42_001350"
     CDS             complement(2509..3951)
                     /gene="gatB"
                     /locus_tag="VVE42_001350"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEU3073537.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDIHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEVELEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEVQRQAEILRSGGQIRQETRRYDEANKATILMRVKEGAADYRYFPEPD
                     LPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LGGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMIAIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAVLIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVALKLLAQELAKLKEN"
     gene            complement(3951..5417)
                     /gene="gatA"
                     /locus_tag="VVE42_001351"
     CDS             complement(3951..5417)
                     /gene="gatA"
                     /locus_tag="VVE42_001351"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEU3073538.1"
                     /translation="MTFNNKTIEELHNLLVSKEISATELTQTTLENIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDADNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAIASEDAKDSTSAPVRIADFTSKIGQDIKGMKIALPKEYLGEGINPEVKE
                     TILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKYSEETIYQVAAAFEATTDYHKQQPVIFGGDN"
     gene            complement(5417..5719)
                     /gene="gatC"
                     /locus_tag="VVE42_001352"
     CDS             complement(5417..5719)
                     /gene="gatC"
                     /locus_tag="VVE42_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEU3073539.1"
                     /translation="MKITQEEVTHVANLSKLRFSEKETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVAEEGTDRDRLFKNVPEKDNYYIKVPAILDDGGDA"
     gene            5932..7476
                     /locus_tag="VVE42_001353"
     CDS             5932..7476
                     /locus_tag="VVE42_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEU3073540.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLETFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVTNAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMNPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            7904..8101
                     /locus_tag="VVE42_001354"
     CDS             7904..8101
                     /locus_tag="VVE42_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HEU3073541.1"
                     /translation="MKEEKKAIVLGADNAYMDKVETTIKSLCVHHYNLKFYVFNDDLP
                     REWFQLMEKRLETLNSEIVNV"
     gene            8212..8388
                     /locus_tag="VVE42_001355"
     CDS             8212..8388
                     /locus_tag="VVE42_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HEU3073542.1"
                     /translation="MDSDIVVRKSIDELWDLDLTAIPLAAVRDDFYTHNFNSGVLLIN
                     NGMWRAENVTQDLI"
     gene            8504..8692
                     /locus_tag="VVE42_001356"
     CDS             8504..8692
                     /locus_tag="VVE42_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEU3073543.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
     gene            8849..9214
                     /locus_tag="VVE42_001357"
     CDS             8849..9214
                     /locus_tag="VVE42_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU3073544.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            9217..10884
                     /locus_tag="VVE42_001358"
     CDS             9217..10884
                     /locus_tag="VVE42_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEU3073545.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDTVEVMRAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFYSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALSD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
BASE COUNT         3136 a         2075 c         2479 g         3226 t
ORIGIN      
        1 tttaagaagg tacttgcaat gacaggtaca aatacattta cagttctttc aactgaggac
       61 ttggagcaaa cttcaggtgg tcttgctgtt tgggaagatg gatatagtag atggttatat
      121 tatagagaat ttgctcccta tatgaggcaa ggggcactta attcttatat agatgcttgg
      181 aagtacggct tccgagcagg gtaatctttc aaaaaagtac gatgagaaac acaaaaaaac
      241 taagacaatt tggaatattt ttgcttatta ttttactatc tacctattta ccacaaacaa
      301 ttggattgta tgtgacgatc attttagggt taggagctga tgtctattct cttattttaa
      361 caatgggatt agtgggaagt ttccttctat taatttggag gttaaaaaag aaaaagatgc
      421 tctttatctt tgagaaaaag agctggaact ggtcatttgt cttctactta tttgcaactt
      481 atgtggttta tcagattctc ggtaattttt gggcacgcta tgctcatttg attaatcata
      541 ggaatattca tgatgagtat tttaccgtgc ttttttcaaa tggacaacca acctttttat
      601 cgactatctt atcttttgtc ctgcctgtaa tcatcggtcc tgtttttgag gagactcttg
      661 atagagggta ttttatgaat accttctttc ctaagtcaaa gtactattta gatgtcatct
      721 tatcaggcct tatctttggg cttagtcatt tgatactatc tcacagagat ccaattagtt
      781 tattatatta tagtttgatt ggtttctttt ttgcccttgt ttatcgttct accgacaatc
      841 tgagattaac gattctctgc catagttttt ttaactttct caatcatgca aaacctatct
      901 ggatttttgt ttacaattat atctattatc atttttttag atagtgggag gcaaagaaaa
      961 aagtgtttga tatctatcaa acactttttc tttttattgt tcttctgtta caaactggct
     1021 gagcagtcca ttgataaaac gggcagattt ttgatcggag aagtcctttg caagctcgat
     1081 agcttcatta acagcaacca gctgaggagt gtcaaatgaa gtgatttcaa agactcccaa
     1141 gcgaaggagg tttctctcca cgagcgttaa gcgttcaatg gtccaacctg cttttaaatg
     1201 ctgagtgatt tgcttatcta gttcttcctt tttagcttga acaccagaaa cgaggtctat
     1261 caaaaaggct ggaagttgta catccgtatc ttcacgatca tgagtatagg cgaaacgaca
     1321 agcagtttcg acatccgtac cgaactcaag gctcatgaga gcttgaaaag cgcatttacg
     1381 gagttggcgt ctagattcta atagtggact agtcattgag gaagtcctcg tcaaatagat
     1441 ctttcaattc tggttttggt gttttatctg ggacgatacc tgcaacgtga atattgatag
     1501 cagcgagttc tacatcagcc atattacgga cggcatcttt gacagctttc tggatagcaa
     1561 ccgctacctt aggaactttt actccgtact caaggtagag atagatatct gctgtgagtt
     1621 cttcgtccac gtttttaaga taaatgccac ggccgagtga aagttttgaa agggtatcag
     1681 acactgatct gtttgaaaaa gagtgaacac cctctacctt tgcagtagcg atagcaatga
     1741 ttttttcaag tacacgtggg gcgataacga tttcgccaag ttgttcttca attcccatag
     1801 aatgacctct ttctagagat taggcacgag aaacgtaagt tccttctgca gtgttgataa
     1861 cgagtttttg tcctgcttcg atgaagtctg gaacgtttac gacaagtcca gtttccatcg
     1921 ttgctggttt accagaacct gtaacagtag cacctttgat agatggttga gtttcagcaa
     1981 ctgtcaactc aacagtagta ggaacggtga caccgatcac ttcagttccg tagaattgga
     2041 ttttcacatc agagttttca aggatgtaaa gcaattcgtt ttcaacattg actacaggga
     2101 tttcgtattg gtcgtaagtt tctgtattca tgaagtatgc tgtgtcatcc attttgtaca
     2161 agtattgagc tgggacagtc tcgataatag cttgttcaaa tttttcctct ggacggtagc
     2221 ttgtgtcaaa tgtagaacca gtacggacat cacgcaattt catacgcatg atcgtgtttc
     2281 ctttacctgg tttgtggtga ctagcttcca aaacgcgaat caatttgccg tcagctgttt
     2341 caaaggtcat accagctttt aatttacttg cttcaatcat gtttttacct ctttaataaa
     2401 tattattact cttcatttta ccataaaata ctctggaaat aaagccaaaa tagttattgg
     2461 gagttggtag agaagaaaaa accaacctta gggctggttt tgttctgtct agttttcttt
     2521 caacttcgcc aattcctgtg caagtagttt aagggcaact tgtgggttgg cttggccttt
     2581 ggttgccttc ataaggaatc ctgtaaaggc tttgtcggcg ttacgtttgc ctgacttgaa
     2641 gtcggcaaca gcagcttcgt tatccgcaaa gacttggtgg ataattggaa tcaaaacagc
     2701 tggatctgaa atttgaacca tacctgcttt ttccacgtat tcacgcgcgc caccgccatt
     2761 tttagctaga tggacaaaga ctttcttggc aatcttagat gaaatagtac cgtcttcgat
     2821 gatggcaatc atttcaacca agttttctgg tgtcaattcg atttgttcca gtgttttacc
     2881 ttcagcattc aagaactgag cgacttcccc ttggagccag ttagagactt gtttggcatc
     2941 accacctagg gcaacagctt tttcaaagaa gtcagaagtg actttattag cagtcaactg
     3001 actagcatcg tagtctgata aaccaaggtc agatacataa cgcgcacgac gttcttttgg
     3061 aaactctggc aactcagtcc gcatttcctc aatccactcg tcagaaattt caaagagggg
     3121 taggtctggt tctgggaagt agcggtagtc agcagcccct tccttgacac gcatgaggat
     3181 ggttgcttta ttcgcttcat cgtaacggcg tgtttcttgg cggatttgac cacctgagcg
     3241 aagaatttca gcctggcgtt ggacttcgta ttcaagacct ttacgaacgt ttgagaagga
     3301 gttgaggttc ttcaattcag tcttggtacc gaatttctct tgaccataag gacgaaggga
     3361 gatgtttgca tccacacgca tcgaaccttc ctccatctta acgtcagaaa tgccagcgta
     3421 ctggataact tccttgagtg ctgtcagata agcataggcc tcctctggcg aacgcatatc
     3481 tgcctcagat acaatctcaa tcaagggaac cccttggcgg ttgaggtcaa cataagagta
     3541 gccatctgta ccatgggtgt ttttaccagc gtcttcctct aggtgggcac gttcgatacc
     3601 gattttcttg gtcgtaccgt cttctagttc gacttcaatc cagccattat atccgattgg
     3661 ttcatcaaat tgagaaattt ggtaggcttt ggggttatca ggatagaagt agttcttgcg
     3721 gtcaaagtgc atctttttgt ggatgtccat gttgagggca agagcagcct tgattccggc
     3781 atcgacaacc cctttattga gaactggcag aactcctggg aaagaccagt caatcacgtt
     3841 agtgttggca ttttgatcat ttccgaagtg ggcagaagta ggtgagaaga tttttgaatt
     3901 ggtgttgagc tctacgtgga cttcaagtcc gatgactgtt tcaaagttca ttagttgtca
     3961 cctccaaaaa tcacgggttg ttgtttgtgg tagtctgttg ttgcttcaaa agcagcagca
     4021 acttggtaaa tggtttcctc agagtacttg ggaccaatca attggagtcc gacaggtaga
     4081 ccttgagaga atccagcagg aatcgaaatt ccaggcagtc ctgccaagtt tacaggtatg
     4141 gtcaataggt cggctaagta catggcaact gggtcatggt tgagtgagtc caagtcatag
     4201 gcaacacttg gagcagttgg acccaaaatc aaatcgtaat ccgcgaagac tttttcgaaa
     4261 tcttgaatga tgagggtacg gacttgacca gcctttttgt agtaggcatc atagtaacct
     4321 gatgaaagac tgaaagtacc cagcatgata cgacgtttta cctcttcacc aaagccttgg
     4381 cttcggctgt ttacatagat ttcatcaagg ttggttgcat cttctgcgcg atagccgtaa
     4441 cggataccgt caaagcgttg caagtttgat gaagcttctg atgaagcgat gatgtagtat
     4501 acggcaactc cgtatttaga gtgaggaagg ctgacttctt cgacaatagc acccaatttt
     4561 tcaaagtgtt tggccgcgtt taagattgtt tccttaacct ctgggttaat tccttcaccg
     4621 aggtattcct taggcaaagc gattttcata cccttgatgt cttggccgat ttttgaagta
     4681 aagtcggcga tgcggacagg agcagaagta gagtctttag catcttcgct ggcaatagcg
     4741 ttgagcaaga gggcattttc cttaacagta ggagcaaaag gtccaatctg gtctaatgag
     4801 ctgccaaagg caatgagacc gaaacgtgaa actgttccgt aggttggttt gagaccaacg
     4861 attccgttga aggcagcagg ttggcggatg gaaccaccag tatcagaacc aagtgacaag
     4921 cgaacttgtc ctgaggctac agctgcggca gaaccacttg atgacccacc aggaaccttg
     4981 ctgtggtccc aagcgttttt agttgctccg tagtgtgaag tttcacctga accacccata
     5041 gcaaattcgt ccatgttggt ctttccaacg acaatcatgc ccttggtttt tgcattggca
     5101 acagctgtcg catcaaagat tggctcatag ttgtagagca tttttgaggc agcagttgtg
     5161 agaataccgt ctgtagagat gttatcctta acagcaagtg gaattcctga aaggacattg
     5221 tcagcatcaa ttccagcttc atcaatggct ttagcttgaa caagagcttg ctcctcagcg
     5281 atggtgacaa atgaattgag agcttcctca cgagacttga tattttcaag tgttgtttgg
     5341 gtcaattctg ttgcagaaat ttccttagag acaaggagat tgtgcaactc ttcaatagtt
     5401 ttattgttaa aagtcattag gcatctcctc catcgtctag gatagctggc accttgatat
     5461 agtagttgtc tttttcaggt acgtttttaa acaagcgatc acggtctgtt ccttcttcgg
     5521 ccacatcagg gcggagtaca gtcttgcggt cagccatagt cgtagtaggt gcgacaccag
     5581 ttgtgtcaac ttcgcccagc aattcaacca tgtcaacaat cttagacaag gtggtcgcaa
     5641 aggcagcagt ttctttttca gagaatctta attttgaaag attggcaacg tgtgttacct
     5701 cttcttgcgt aattttcatc ttgcatcctt tcgtgaaatg atgattttta gtctgttcta
     5761 ttttaccata ttttcctata aataagggag ggggaagtga aaagaaatag aaattgaaaa
     5821 aaatgctaaa atccgatata atggagtgtt gaaaggaatg gtaaaatcca atgtaaaaat
     5881 cattctcagc tattgaaaca agaaaaatag agaatcaaag aaagagaact tatgaatatt
     5941 caagaagaaa ttaagaaacg tcgtaccttt gccattatct cccacccgga cgcggggaaa
     6001 acaaccatca ctgagcagtt actctacttt gggggtgaga ttcgtgaggc tggtacggta
     6061 aaagggaaga aaacagggac ttttgctaaa tctgactgga tggatatcga gaagcaacgt
     6121 gggatttctg ttacttcatc tgttatgcaa tttgactacg acggcaagcg cgtgaatatc
     6181 ttagacacgc cagggcacga ggacttctca gaagatacct atcgtacctt gatggcggtg
     6241 gatgctgcgg tcatggtcgt ggactctgcc aaggggatcg aggctcaaac caagaaattg
     6301 tttgaggttg tgaaacatcg tggcattcca gtctttacct ttatgaacaa gttggatcgt
     6361 gacggtcgtg agcctttgga tctcttgcaa gaattggaag aaatcttggg cattgctagc
     6421 taccctatga actggcctat cgggatgggg aaagcctttg agggcttgta tgacctctat
     6481 aaccaacgtt tagagcttta caaaggggat gagcgttttg ctagcctaga agatggggac
     6541 aaactttttg gtagcaatcc tttctacgag caagtcaagg atgacattga gcttttaaat
     6601 gaagctggga atgagttttc agaggaagct attctggctg gagaattgac gcctgtcttt
     6661 ttcggttcag ccctgacaaa ctttggtgtg cagaccttcc ttgagacctt cctcaagttt
     6721 gctccagaac cacatggtca caagaaaaca gacggtgaaa ttgtggatcc ttatgacaag
     6781 gatttctcag gctttgtctt taaaatccaa gccaacatgg atcctcgtca ccgtgaccgt
     6841 attgcctttg tccgtatcgt atcaggcgaa tttgagcgtg gcatgagtgt caatctccct
     6901 cgtactggta agggtgccaa actatctaat gttacccagt ttatggcgga gagtcgtgag
     6961 aatgtgacca atgccgtggc aggtgatatt atcggggttt acgatactgg tacttatcag
     7021 gttggggata ccttgacggt tggaaaaaac aagtttgaat ttgaacccct gccaaccttt
     7081 acacctgaga ttttcatgaa agtttctgct aagaatgtta tgaagcaaaa atccttccac
     7141 aaggggattg agcaattggt gcaagaagga gccgttcagc tttataagaa ttaccaaaca
     7201 ggtgagtaca tgctaggcgc tgtaggtcaa ctccagtttg aagtctttaa acaccgcatg
     7261 gaaggcgagt acaatgcgga agtagttatg aacccaatgg gtaaaaagac cgttcgttgg
     7321 atcaagcctg aggacttgga tgaacggatg tcgtcaagtc gcaatatctt agccaaagac
     7381 cgttttgacc aaccagtctt tctctttgaa aatgactttg ccctccgctg gtttgcggac
     7441 aagtatccag acgtagagtt agaggagaag atgtgactca gtaccaacaa ttggaactaa
     7501 agttccaaat tgttggacgc tagtcgctat ttggcgaact agctaactgc ctcactaact
     7561 acgtttggca aatagaatcg atttgtaaaa ctccgtgtcg taatctaata atcggtaacc
     7621 gctatggcgg cttacctaaa cgcttcacta ggaaaaatcc acgaatggta ttgatttgtg
     7681 gatttttccg tcgtagcaga aagtaggtag cctagctaac tgccttacta actacgtttg
     7741 gcaaatgaaa tcgatttgta aaactccgtg tcgtaatgta aaaattgtaa accgtaatgg
     7801 gacttcatct aaaatctcca ctggtatggt tttacgaacg aataggcgaa aataatggtt
     7861 acgtagaaac atatgatggg aataaaaaga aatgagataa agagtgaaag aagagaaaaa
     7921 agcgattgtc ttaggtgcag ataatgctta tatggataaa gttgaaacaa caataaaatc
     7981 tctttgtgtt catcattata atcttaagtt ttatgttttt aatgatgacc ttccgcgaga
     8041 atggttccaa ttgatggaaa aaagattaga gactttgaat agtgagattg tgaatgttta
     8101 gattgacagt agtattctga aaggttatcg tctccccttt gagggacttt catatgcggc
     8161 ttttttaggt attttattcc aaagtatgta agcgagtctc gtgtactgta tttggattca
     8221 gatattgttg taagaaaatc gatagatgag ctgtgggatt tggacttgac ggctataccc
     8281 ttagctgcag ttagagatga tttttataca cataatttta attctggtgt tctgttaatc
     8341 aacaatggca tgtggagagc agaaaatgtc acgcaagacc tgatttaaga cacaaataaa
     8401 aaatcttcca attcccttgc aaaaaacaag aaagtgtgat aacatagtac ggtatgtggt
     8461 gctagcacat ccgctatatt agatctaata ggaggaaaac acaatggcta aagtatgtta
     8521 ctttacaggt cgtaagactg tatcaggaaa caaccgttca cacgcgatga accaaacaaa
     8581 acgtgccgta aaaccaaacc ttcaaaaagt tactgttctt atcgatggta aacctaaaaa
     8641 agtttgggct tcagctcgtg ctttgaaatc aggtaaagtt gaacgcgttt aataataaaa
     8701 gcaaaggaga ccttagggtc ttttttcttt tattgtgggt ataacatcgt ttgaaaaata
     8761 gagtaaatat ccgctgatac atcattctgc ttttacactt gggctgaaat atgataaaat
     8821 agagtatcaa ctagttgagg taaaaaggat gactgtaaaa attaatacaa aagatggtca
     8881 aatcgaacta acagatgaag tgattgcaac cgtcgtaggt ggagcagcaa ctgagatttt
     8941 tggtgtggtc ggtatggcta gtaaaaatgc cctcaaagat aatttccaag ccctgctagg
     9001 taaggaaaat tattctaaag gtgtcgtcgt aaaggcagcc gaagatggca gtattgcagt
     9061 tgatgtatat accgtgttaa gctacggaac aaagattagt gaagtgtcaa aaaacattca
     9121 agagcgtgtt gcctttagct tggaaaacca gcttggaatt actgctcaga ctgtaaatgt
     9181 ctacattcaa aatatcaaag ttgtaggaga ataaccgtgt caaagattac tactagctta
     9241 tttcaagaaa tggtgcaggc tgcatcaact cgcttgaata agcaagctga atatgtcaat
     9301 tcattaaacg tctttccagt tccagatgga gatactggga caaatatggg aatgaccatt
     9361 gaaaatggtg ctaaagaagt tgcagacaag ccagcttcta cagttggaga agtagcgagc
     9421 attcttgcca aagggctttt gatgggtgcg cgtgggaact caggagtgat tacatctcag
     9481 cttttccgtg gattttccca agctatcaag gataaagacg agttaacagg tcaagacttg
     9541 gctctggcct tccaatcagg tgtggaagtt gcctataagg cagtgatgaa acctgttgaa
     9601 ggaacgattt tgacagtttc tcgtggagct gctatcggtg ctaagaaaaa agctgagcaa
     9661 acagatgata ctgttgaagt catgcgtgca gccttggaag gtgctaaaac agctctagct
     9721 aaaacaccag acatgcttcc agtattgaaa gaagttggcg ttgtggactc aggtggtcaa
     9781 ggattggtct tcatctacga aggtttcctt tcagccctta ctggcgaata tattgcatct
     9841 gaggactttg tagcgactcc tgccaacatg agtgagatga tcaatgtaga gcatcataag
     9901 tctgtagctg gtcacgtagc gactgaggac atcacgtttg gttactgtac tgaaatcatg
     9961 gtagctctta agcaaggtcc aacctatgct aaagattttg actacgatga attccgtaac
    10021 tacttggatg agcttgggga ttctctcctc gttgttaacg atgatgaaat tgtcaaagtc
    10081 catgttcata cagaagatcc aggacttgtt atgcaagaag gtctcaaata tggtagcttg
    10141 gtcaaggtaa aagttgacaa tatgcgtaac caacacgaag cacaggttga gaaagaagct
    10201 actcaagtta tcaagtcggc tgaagaaaaa gagtatgctt taattgctgt ggtggctggt
    10261 aaaggtctag cagatatctt ctattctcaa ggcgtggact atgttatcga aggcggtcaa
    10321 accatgaacc cttcaacaga agactttatc aaggctgttg aacaggtcaa tgcccgtaac
    10381 atcatcttct tgccaaacaa caagaacatc ttcatggcag ctcaatctgc ggcagaagtt
    10441 ttggagcaac cagcagtagt ggtagaggct cgcactcttc ctcaaggtat gacaagtctt
    10501 cttgcctttg atccaagcaa gtccattgaa gaaaaccaag agcgtatgac agctgctctt
    10561 agcgatgtcg ttagcggaag cgtcacaaca gccgtgcgtg atacaacgat cgatggctta
    10621 gaaatccatg aaaacgataa tctaggtatg gtggatggaa aaattcttgt gtcaaaccct
    10681 gatatgcacc aaacattgac tgaaaccttg aaacatatgt tggatgaaga cagtgaaatc
    10741 gtaaccttct atgtcggtga agacggaagc gaagaacttg ccaatgaaat cgctcaagaa
    10801 atcgtagaag aattcgaaga cgttgaagtc gagattcatc aaggtcaaca acctgtttac
    10861 ccatacctat ttagtgtgga ataagattaa tgaaggactg agaaatcagt tctttt
//
LOCUS       DAVGJL010000067        10840 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.067, whole genome shotgun sequence.
ACCESSION   DAVGJL010000067 DAVGJL010000000
VERSION     DAVGJL010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10840
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.067"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(148..1470)
                     /locus_tag="VVE42_001359"
     CDS             complement(148..1470)
                     /locus_tag="VVE42_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEU3073546.1"
                     /translation="MLELLKSIDAFAWGPPLLILLVGTGIYLTIRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFAALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDHGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVALLGIG
                     TFTQVNSIAESIQNTTTISPAITALVLSVFVAIAVFGGLKSISKVSTTVVPFMAIIYI
                     LGTLTVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILVTGVWSGDLNGVALT
                     QSAFSTVFSHFGPALLTIFLVLFAFTTILGWNYYGERCFEFLFGARFIWLYRVVFVLM
                     VLLGGFIELDMVWIIADIVNALMALPNLIALLVLSPVVIAETKKYFDK"
     regulatory      complement(1561..1649)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(1813..4149)
                     /locus_tag="VVE42_001360"
     CDS             complement(1813..4149)
                     /locus_tag="VVE42_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEU3073547.1"
                     /translation="MNKKILETLEFDKVKALFEPHLLTEQGLEQLRQLAPTAKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGTDLNIEEFLLLKRVLLASREL
                     QSFYANLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEELARIRRKIHD
                     SESQVRDVLQDLLKQKAQMLTEGIVASRNGRQVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHATEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVHFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADRGSRVGIFEEIFADIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQIDQD
                     NDVNRIIEQLEEQTLESRKRLDNIREVEQENLKMNRALKKLYNELNREKETELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKAKLKKLAPEKVDLSKNKVLQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQVGLIKMTLEEKEFDLVQAQQEKPV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYEEAMNELDIFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     gene            complement(4253..4801)
                     /locus_tag="VVE42_001361"
     CDS             complement(4253..4801)
                     /locus_tag="VVE42_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEU3073548.1"
                     /translation="MISLLLLLVLVWGFYIGYRRGLILQVYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEGQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIVYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPITTSWLKQIWVTNLIG"
     gene            complement(4798..5100)
                     /gene="zapA"
                     /locus_tag="VVE42_001362"
     CDS             complement(4798..5100)
                     /gene="zapA"
                     /locus_tag="VVE42_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEU3073549.1"
                     /translation="MANLNRFKFTFGKKSLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSADDETIALLLAVNCLSTQLSREIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            5187..6059
                     /locus_tag="VVE42_001363"
     CDS             5187..6059
                     /locus_tag="VVE42_001363"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEU3073550.1"
                     /translation="MASITLTPSEKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFGYQAVEQTSGQNLPLIGTDEVGNGSYFGGLAVVASFV
                     TPDQHAFLRKLGVGDSKTLTDQKIRQIAPILKEKIQHQALLLSPSKYNEVIGDRYNAV
                     SVKVALHNQAIYLLLQKGVQPEKIVIDAFTSAKNYDKYLAQEANRFSNPISLEEKAEG
                     KYLAVAVSSVIARDLFLENLENLGRELGYQLPSGAGTASDKVASQILQAYGMQGLNFC
                     AKLHFKNTEKAKNA"
     gene            6071..6685
                     /gene="lepB"
                     /locus_tag="VVE42_001364"
     CDS             6071..6685
                     /gene="lepB"
                     /locus_tag="VVE42_001364"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEU3073551.1"
                     /translation="MNLFKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKHLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRFWPITRIGTF"
     gene            6765..6887
                     /locus_tag="VVE42_001365"
     CDS             6765..6887
                     /locus_tag="VVE42_001365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorohydrolase"
                     /protein_id="HEU3073552.1"
                     /translation="MKIKEQTRKLAVGCLKQCFEVVDRTDEVSSKYCFEVADGS"
     gene            6964..9330
                     /locus_tag="VVE42_001366"
     CDS             6964..9330
                     /locus_tag="VVE42_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEU3073553.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYTFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGIAGLSAKNREAFVSTLRLNYGTEMVLAKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEARDLLEQTLTLLESSRPVELDPSQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLIRILEKGEQKSHDLETIQKHITTIEEELGIEYDN
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDFRKKSNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDADFIIVDECSMVDTWLANQL
                     FSNISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTRLEKIYRQSKESTIVTLA
                     SQIRQGILPADFTQKKADRSYFEIASGHIPATIEKILGAALRNGIPARDIQVLAPMYR
                     GTAGIDAINQLMQDLLNPPQKDQLSFEAPQCHYRKRDKVIHLVNDAEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAITRAKSKLILLGELQAFDYATQHIGTARKTYLIERFSDLLEN
                     VEEKQQAVSETVTSSASEQSYILTEENWDSIPAMIGITDTDLKEIFGK"
     gene            complement(9378..10661)
                     /locus_tag="VVE42_001367"
     CDS             complement(9378..10661)
                     /locus_tag="VVE42_001367"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEU3073554.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDKKFGEESLYQDVMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVIAAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
                     ENGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYRKEL
                     AAAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQNLLSADMLKHDITIKKAVELITSTATVK"
BASE COUNT         3097 a         2454 c         2198 g         3091 t
ORIGIN      
        1 ttatctaaga taaagcttta ttctctcata agattttcgt tgttatatta gtttatcaca
       61 cttccaatca cttgcataat atatattata tatcaggctg ataaaaatta tttataggca
      121 aaaaatcaca cgagctgtgt gattccatta tttgtcaaaa tactttttag tttcagcaat
      181 aacgactggc gacaagacca agagggcaat caagtttggc agagccatca aggcgttaac
      241 gatatctgcg ataatccaga ccatatccaa ctcgataaat cctcctaaca agaccatgag
      301 cacaaaaacc acacggtaga gccagataaa gcgagcccca aagaggaact caaaacagcg
      361 ttctccgtaa tagttccaac ctagaatcgt tgtaaaggca aaaagtacaa ggaagatggt
      421 caagagggca ggcccaaagt gtgaaaagac tgttgagaaa gctgactgag tcaaggcaac
      481 cccattcaag tcaccactcc aaactccagt taccaagatg gtcaaaccag ttagagtaca
      541 aatgatgagg gtatcaataa aggttcctgt catggaaatc aaaccttgct ctactggttc
      601 atttgtcttg gcagctgcag ctgcaatagg agcagaaccc agaccagatt cgtttgagaa
      661 cacaccacgc gccacaccat tttgaatagc catccgaacg ctagcaccag caaatccacc
      721 taccgcagca aggggactaa aagctgaggt aaagactaaa gcgattgtgc cagggatttt
      781 tccaatatta aagaaaataa ctgtaagagt tcctaagata taaatgatgg ccataaaagg
      841 aacaacagta gttgaaacct tagaaataga cttgagtcca ccaaagactg caatcgctac
      901 aaagacagac aagacgagag ctgtgatggc tggcgaaatc gtcgttgtat tttggataga
      961 ttctgcaatc gagttgactt gggtgaaggt tccgattccc aagagagcaa ccaatactcc
     1021 tgctactgca aacagaacag caagtggtcg ccacttttct cccatcccta gaaggatata
     1081 atgcatggga cctcccgcta ctgcaccatg gtcgtccttg gtgcggtatt tgatggccaa
     1141 gagtccttcc gcatacttgg tagccattcc aaagaaagcc gccatccaca tccaaaatag
     1201 agctcctggt ccaccaacct tgatagccgt cgcaactcct atgatatttc ctgttccaac
     1261 agttgatgcc aaggctgtac acagagctgc aaaactggat acatcaccat gtcccttatc
     1321 ctggataaaa ataagctgaa aagccttggg cagacgcaaa acctgcaaga gtcctagccg
     1381 aatagttagg taaatccctg ttccgaccaa taaaatcaag aggggcggtc cccaagcaaa
     1441 agcatcgatt gatttaagca attctaacat ttccttctcc tatcgtttca accccaaaag
     1501 aaagagcaca tgcaagatac atgtactctg gaatgcttag ataaatgcta aaaagcggtc
     1561 tatcctagct ctgtcctttt acctgagagt ttgagcagtt gcctgccttg ccccttcggt
     1621 gcctttacgg tctctccaga gttccgtcca tttacagtca tggaaaatca aacgattccc
     1681 cacttctatt aaacttcatt cggtgttggt atttaattga ttctaatttt acaaaaaatg
     1741 ttggcttttg tcaatgtgtt tattagtaaa aattagttca acagttttta ctttataaag
     1801 tccagaatac tgctatcctt taaaagtgac aatagtcgca ccactgcctc cagcattttg
     1861 tggggcatag ccgaaactct tgacatgttt gtttctttgc aagtatttgg taactccttc
     1921 acggatgact cctgttccga taccatggat gatatcaact tgagccatat tgttaagcaa
     1981 ggcttggtcg atgaagatat ctagctcatt catggcttct tcatagcgct tgcctcgaag
     2041 atccagtcta gcttgaggtc ctcgcccaga agttcgtttc acaacattga cctgcttctt
     2101 cttgactggt ttttcttgct gggcttgaac aagatcaaac tctttctctt ccaaggtcat
     2161 cttaatcaag ccaacttggg cttcccagcg gccgtccttg agttgactgg tcaaggtacc
     2221 acgctgacca taactgagaa ccacgatatc atctcccacc tttggagctc gttttttctt
     2281 ggccttttga aggaccttgt ttttagacaa gtccactttt tcaggagcca attttttcaa
     2341 cttggctttg gcttcaatga tttcgtgggg cttgagttgg gatttactgt ggagattttt
     2401 gagaatctgg tcactttcac ttagggccat atccacaatc tcagcagcct gttcacgcgc
     2461 cttgttaagc tcggtttcct tttcacgatt aagctcgttg tagagttttt ttagcgcacg
     2521 gttcatcttg agattttctt gctccacctc acggatattg tccaagcgtt tgcggctttc
     2581 cagcgtctgc tcttctaatt gctcaatgat acgattgacg tcattgtcct gatcgatctg
     2641 ctgactggca tctcctacga taacttcaga taggcctaga cgtttggcaa tttcaaaggc
     2701 attacttcgg ccaggaacac cctgcataaa gcgataggtc gggcgaagag ttgcagtatc
     2761 aaactccata ctggcatttt gcacaaaggc tgtctcaata ccgtaggcct tgagttctgg
     2821 atagtgggtc gtcgccatgg tcttgatttg acgcaggcga aggtcctcca gaatagccat
     2881 ggcaagggct gctccctctt ggggatcagt accagccccc aactcatcca aaagtaagag
     2941 tgaatgttgg ttgaccttgc caagaatatc cacgatattg gtcatatggc tagagaaggt
     3001 agacaagctc tgctcaatag actgctcatc tccaatatca gcaaagattt cttcaaaaat
     3061 accaacacga cttcccctgt ctgctaaaat cggcaatcct gactgggcca tgacctgtgt
     3121 caagcccaga gttttgagca tgatggtctt cccacctgta ttgggacctg taatgacaat
     3181 agctgttaaa tcttgaccaa aatggacatc atttgcgacg gcatttttga ccaaaggatg
     3241 gcagacatgg agcagttgaa tctcttgatt ttctgacagc tgaggcacga ctgcttgtct
     3301 ttcttggata aatcgaacct tggcacgaat caagtccaga tgaccgataa tccaagcgtc
     3361 attagcaatc tcagtcgcat gagggcggac acgctcagaa atttcttgga gaatgcgaag
     3421 catttcatag cgctcatctg ctcgcagact agcaatttct tcgctcagtt tgactacctc
     3481 acgtggttcg atatagacgg tgtttccact agcagaaata tcatgaacga cacctgcaat
     3541 cttattgcgg taggtgtttt tgactggtaa aacctgacgg ccatttctgc tagcaacaat
     3601 tccttctgtc aacatctgcg ctttttgctt gagcaagtct tgtaaaacat cgcgtacctg
     3661 actctcgcta tcatgtattt ttcgacggat tcgcgccaat tcttcactgg caaaattttc
     3721 aatgaaaccc gcatcattaa aggcctgaag atttccttgt aattgcggaa aatcatgtaa
     3781 tttctcaaac caaagggcta attcttccaa gctgacattt tccagattgg cgtaaaaact
     3841 ttgaagttct cggctggcaa gaagcacgcg tttcaagagt aggaactcct cgatattgag
     3901 atccgttccc atctccaacc tcttgcagac tcctgcaatt tccttagttg agagaatagt
     3961 aaaatgcggt tgctcgacga aaagagcctg catttctttc atctcagcaa aagcctgttt
     4021 gattttatct gctttggcag tcggagccag ctgtctcaat tgctccaagc cctgctcggt
     4081 caacaaatga ggctcaaaca aggccttgac cttatcgaac tctaatgttt ctaatatttt
     4141 cttattcatc tttatttcct tgtctttgaa tgtctaggtg tggtagcttt ttacattctg
     4201 atagattcga gtcaaatctg taaacaaagg gctaggaaaa ctcctgccct ttttatccga
     4261 ttaaatttgt cacccagatt tgtttgagcc aactggttgt tatcggtatg ctctggatga
     4321 tgtgttttgc gacgatactc ttttcaagag gattttgtat aactgccatg gggatggtcg
     4381 ccaagattgt caaggccatt tgcaagacaa ataaggtcac caacatggac aagatacctg
     4441 ctgaaacttg gaacaactta ccacccagtt ttttgctagg aatcaagtgt aagagaagac
     4501 caagcaaacg accaatgcta tagacaatcc caaatacaag caagtagccg atacctgcat
     4561 aaaagacctt atccagctga aagagttgat ccgatgggaa aaagaaagtc ccctgacctt
     4621 cctgcgaatt tgcataaggg agcaataaat ggaattgctc tccaagcccc ttataaaact
     4681 ggccagccat aaaagccgat gccatggctg aaatcaggta ataaacctgt aagatcaggc
     4741 ctctccgata gccgatataa aatccccaaa ccaagaccaa tagaagaagg agtgaaatca
     4801 taaggaatcc tcaatcttgc tctgttcttg cttacaagtc acaagcttgt gacggagttc
     4861 ttctagctct tgctccttat cgtcaaattc aatctcacgg ctgagctgag ttgataaaca
     4921 gttgactgcc aacaaaagag cgattgtttc atcatctgcg ctaggcattt gttctttaat
     4981 tgcttggtat ttttctgtcg caaccttagc gatttcctcc ataaaaaggt tatcatgttc
     5041 gcttgtcaag gttaacgatt ttttcccgaa tgtaaatttg aatcgattta gatttgccat
     5101 aaaattcacc tcacgatatt ataccaaaat tcgctaattt tgtcagtttt tacaaatttt
     5161 actgcttttg tggtacaata gaaactatgg caagtatcac actcacacca agcgaaaagg
     5221 atattcaggc ttttcttgaa cactatcaaa ccagtctggc tcctagcaag aatccctata
     5281 tccgctactt tttgaaacta cctcaagcaa cggtttctat ctatacttct ggaaaaatct
     5341 tgcttcaggg tgaaggggct gaaaaatacg ccagtttctt tggctatcaa gctgtagagc
     5401 aaaccagcgg acaaaatctt cctttaattg ggacagatga ggtgggaaat ggttcctact
     5461 ttggtgggct tgcagttgtg gcttcctttg tcacacctga ccagcatgcc ttcttacgaa
     5521 aactcggtgt gggagattct aagactctga ccgaccaaaa gatccgtcag attgctccta
     5581 ttctcaagga aaaaatccag caccaggcac tccttctctc acccagcaag tacaacgagg
     5641 tcatcggaga ccgctacaat gctgtttcgg ttaaggttgc cctccataat caggctatct
     5701 atctcctcct tcaaaaaggt gttcagcctg agaaaattgt gattgatgcc tttaccagtg
     5761 ctaaaaatta tgacaagtac ttggcacaag aggccaatcg tttcagcaat cctatcagct
     5821 tagaagaaaa ggctgagggc aaatacttgg ctgtcgcagt ttcttctgtc attgcgcgtg
     5881 atctctttct ggaaaatctt gaaaatttgg gacgagaact gggttatcag cttccaagtg
     5941 gagctggaac ggcttctgac aaggtggcta gccagatttt gcaagcctat ggtatgcagg
     6001 gactcaactt ctgcgctaag ctacacttta aaaatactga aaaagcgaaa aacgcttaga
     6061 aaggtaagtt atgaatttat ttaaaaattt cttaaaagag tggggattat tcctcctgat
     6121 tctgtcatta ctagctttga gccgtatctt tttttggagc aatgttcgcg tagaaggaca
     6181 ttccatggat ccgaccctag cggatggtga aatcctcttt gttgttaagc acctccctat
     6241 tgaccgtttt gatatcgtgg tggcccatga ggaagatggc aataaggaca tcgtcaagcg
     6301 cgtgattgga atgcctggcg acaccattcg ttacgaaaat gataaactct acatcaatga
     6361 caaagaaacg gacgagcctt atctagcaga ctatatcaaa cgcttcaagg atgacaaact
     6421 ccaaagcact tactcaggca agggctttga aggaaataaa ggaactttct ttagaagtat
     6481 cgctcaaaaa gcccaagcct tcacagttga tgtcaactac aacaccaact ttagctttac
     6541 tgttccagaa ggagaatacc ttctcctcgg agatgaccgc ttggtttcga gcgacagccg
     6601 ccacgtaggt accttcaaag caaaagatat cacaggggaa gctaaattcc gcttctggcc
     6661 aatcacccgt atcggaacat tttaagaaac ctaagaggcc gagaatcacc aatctcagcc
     6721 tcttcttcta tcgtgagaag tgattgttta ttgacaaata ctcaatgaaa atcaaagaac
     6781 aaactaggaa gctagccgta ggctgcttaa aacagtgttt tgaggttgtg gatagaactg
     6841 acgaagtcag ctcaaaatac tgttttgagg ttgcagatgg aagctgacgt ggtttgaaga
     6901 tattttcgaa gagtataaaa taaaaacaat cccaactctc acctattcat gcaaaggaat
     6961 tttatggaag tttatttttc aggaaccatt gaacggatta tttttgaaaa tcccagcaat
     7021 ttttatcgca tcctcctcct agaaatcgac gatacggacg cagaggattt tgatgatttt
     7081 gaaatcattg tcacaggaac catggctgat gtaattgagg gcgaagacta tactttttgg
     7141 gggcaaattg tccagcactc caagtatgga gaacaactgc aaatcagtcg ttatgatcgc
     7201 gcaaaaccaa ctagtaaggg attggtcaag tacttttcaa gtagccattt caaggggatt
     7261 ggtctcaaga cagctcagaa aatcgtagat acctatggcg aaaataccat tgatgaaatt
     7321 ttgcaacacc cagaaaagtt agaaggcatc gcaggactct ctgccaaaaa tcgcgaggct
     7381 ttcgtctcca ctctccgtct caactacgga acggagatgg ttttggccaa actagccaac
     7441 tacggcattc ccaacaaact agcctttcag attcaagact tttacaagga agaaaccctt
     7501 gatgtggttg aaaattatcc ctaccagctg gttgaggata tcaagggttt gggctttacc
     7561 attgctgacc aactagctga ggaactaggc atcgaaagtc aggctcctga acgcttccgc
     7621 gccggtctag ttcacagtct ttttcaggcc tgtatggaaa caggggacac ctatgttgaa
     7681 gcacgggatt tgctggaaca aacccttact ctccttgagt cttcccgtcc cgtagaacta
     7741 gaccccagcc aagttgccca agagctctcc tacctgatcg aagaagacaa ggttcagcag
     7801 attgatacca agatctttga caacagcctc tttttcgctg aggaaggcat ccgcagtcac
     7861 ttgattcgta tccttgaaaa aggagaacag aagagtcatg atttagaaac cattcaaaaa
     7921 catatcacta ctatcgagga agagctggga attgagtatg ataacattca aaaacaggct
     7981 atctgtgatg ctatccagaa taaggtcttt atcctgacag gtggacctgg tactggtaag
     8041 acaactgtta tcaatggaat catcgctgtt tatgcccttt tagaaggact tgacttcagg
     8101 aagaaaagca atctgcccat tcttcttgct gctccaactg gacgagccgc tcgccgcatg
     8161 aatgaattga caggtttgcc tagcgcgacc atacaccgcc acttgggaat gacaggtgac
     8221 gatgatacca gtcatctgga agattaccta gatgctgact ttatcatcgt ggatgaatgc
     8281 tctatggtag atacttggct ggccaatcaa ctcttctcca atatctcttc taacagtaag
     8341 atcctcatcg taggtgacag tgatcagcta ccttctgtca gtcctggaca ggttctagcg
     8401 gatctgcttc atattccttt gattcctcag actcgcttgg aaaaaattta tcgacaaagc
     8461 aaagaatcaa ccatcgtcac cctagctagt cagattcgac agggcatctt gccagctgat
     8521 ttcacccaaa aaaaagctga ccgttcctac tttgaaattg ctagtggcca tattcctgcc
     8581 accattgaaa aaatcttagg tgccgccctc agaaatggta ttcctgcccg tgatatccaa
     8641 gttctggctc ctatgtaccg agggacggca gggattgatg ctatcaatca gctcatgcaa
     8701 gacctgctca atcccccaca aaaagatcaa ctcagttttg aagctcctca gtgccactat
     8761 cgtaagagag acaaggtcat tcatttggtt aacgatgctg aaatcaatgt ctttaatggg
     8821 gatttaggag ctatcacaga cctgattcct ggtaaataca ccgaatcgaa acaagacgag
     8881 attgtcattg attttgatgg taatgaagtc tcttaccccc gtaacgaatg gtacaagatt
     8941 cgcttggcct atgccatgag tattcataag tctcagggaa gtgagttccc agttgtcatc
     9001 ctaccgatta ctagtgctag ccgacgtatg ctggagcgaa atctcatcta cacagccatt
     9061 acacgtgcca aaagcaaact catcttacta ggcgaattac aagccttcga ctatgctacc
     9121 caacacatcg gaactgcccg aaaaacctat ctgattgaac gcttcagtga tctactggag
     9181 aatgttgaag aaaagcaaca agctgtctct gaaacagtca catcaagtgc ctctgaacaa
     9241 tcctacatcc taaccgaaga aaactgggac agcattccag ccatgattgg gattacagac
     9301 acagacctca aagagatttt tggaaaatag tacatcagaa aacccaccaa ttcaggtggg
     9361 ttttctgttt attaagatta ttttactgtt gctgtgcttg tgatcaattc aacagctttt
     9421 ttgatagtga tatcatgttt caacatgtca gctgaaagca agttttgaac ttgtgcaact
     9481 tccatgttgt agtctgctgc caattgctca acttcttttt ggatttcttc ttctgaagca
     9541 tcaaatcctt cagctttggc aactgcttcg ataacaaggt tagtcttagt acgtgactca
     9601 gcttctgctt ggtattggtt gtgaaggtct tcttgagtag ttccagtgat ttggaagtac
     9661 atgtcagggt tgatcccttg acgttgcaag ttcccaagga attcatttac tgaacggtga
     9721 acttcttcat ggatcatttc ttctggaagt tctacgattt cagcgttttc tacagctgta
     9781 tcaattgctg caccttcaac tgcatctttg taagcttctt ctttagcagc agccaattct
     9841 ttgcggtatt tttctttcaa gtcagcaagt gtttcaactt cttcatcaat gtcttttgca
     9901 agttcatcgt caagagctgg aacttcttta gcttttactt cgtggatagt tgtcacgaat
     9961 ttagcttctt tacctgcaag gtcttctgct tggtagtctt ctgggaatgt tacgataaca
    10021 tcaacggttt cgccagctga gtgacctacc aattggtctt cgaaaccagg gatgaattga
    10081 cctgaaccaa gtccaagtga gaagttttca ccttttccac cgtcaaattc aacaccgtcg
    10141 atagaaccaa cgaagtcgat cacaacagtg tcgccgtttt cagcagcagc ttccttgata
    10201 accaattcag ccaggttgtt gcgttcgcgt tcgatacgct cttcaacatc agcgtcagtt
    10261 acttcttttt ctacatcaac tgatacttca aggtttttgt agtcacccaa ttttacttca
    10321 ggttttgtaa cgacttcagc agcgataacc cagtcttgac ctttttccat tgaagttacg
    10381 tcaatttttg gttgggcaac cacttcaaga ccagcttctt ttacagctgc ttcataagcg
    10441 tttggcaaaa gagcgttcat aacgtcttgg taaagtgatt cttcaccaaa ttttttatcg
    10501 aagataggac gtggaaggtg acctttacgg aaacctggaa cattaagaga tttcttcact
    10561 gacttgaaga cacggtccaa ttctggtttg atttggtctt gagagatagt gaaagtcaag
    10621 acaccacggt ttgtttcttt gttttcaaat gatacagaca ttctgtcatt tctccttaaa
    10681 attttttaaa tacagtctat tataacattt tctataaata aaatctagac taatgcagcc
    10741 aattatagac aacaagcctt tttcctaact aaaacactca taaagtatag acaattttag
    10801 actactttgg atatcttttg gttagtcatt ttccaaaact
//
LOCUS       DAVGJL010000068        10667 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.068, whole genome shotgun sequence.
ACCESSION   DAVGJL010000068 DAVGJL010000000
VERSION     DAVGJL010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10667
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.068"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(33..1457)
                     /locus_tag="VVE42_001368"
     CDS             complement(33..1457)
                     /locus_tag="VVE42_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEU3073555.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAIERAAYLHKTAAILERDKEKIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGGFEAASKNKLAVVCREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMFKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHAAKQIVAGAF
                     SYSGQRCTAIKRVIVLESVADKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     SLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVAWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSG
                     AGVQGIKYSIEAMTNVKSIVFDVK"
     gene            complement(2002..4281)
                     /gene="pulA"
                     /locus_tag="VVE42_001369"
     CDS             complement(2002..4281)
                     /gene="pulA"
                     /locus_tag="VVE42_001369"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HEU3073556.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKAYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     STSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAPFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLTPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGTYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKIETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTSAFSY
                     SSYDEIYHHVFVHSAIEHSGCLIYEVHGKEHLLVVVNAKSEPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(4393..6351)
                     /gene="ligA"
                     /locus_tag="VVE42_001370"
     CDS             complement(4393..6351)
                     /gene="ligA"
                     /locus_tag="VVE42_001370"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEU3073557.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVSDSEYDRLYRELVELET
                     AYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQARQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KASQLLLQYFHSIENLYQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETG
                     VNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSVSKKTNLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
     gene            complement(6444..7622)
                     /locus_tag="VVE42_001371"
     CDS             complement(6444..7622)
                     /locus_tag="VVE42_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU3073558.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVWLASMGTIGQTVLGMY
                     QISELVTSILVNPFGGVISDRFSRRKILMTADLVCGILCLAISFIRNDSWMIGALIVA
                     NIVQAIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LHMTLLLDSLTFFIAFVLVAFLPKEEAKVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FLLLVASSVNFFFAAFEFLLPFSNQLYGSEGAYASILTMGAIGSIIGALLASKIKANI
                     YNLLILLALTGVGVFMMGLPLPTFLSFSGNLVCELFMTIFNIHFFTQVQTKVESEFLG
                     RVLSTIFTLAILFMPIAKGFMTVLPSVHLSSFLIIGSGVIILSCISFIYVRTHFEKLI
                     "
     gene            complement(7702..8565)
                     /locus_tag="VVE42_001372"
     CDS             complement(7702..8565)
                     /locus_tag="VVE42_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEU3073559.1"
                     /translation="MEHLGKVFREFRTSGNYSLKEAAGESCSTSQLSRFELGESDLAV
                     SRFFEILDNIHVTIENFMDKARNFHNHEHVSMMAQIIPLYYSNDIAGFQKLQREQLEK
                     SKSSTTPLYFELNWILLQGLICQRDASYDMKQDDLDKVADYLFKTEEWTMYELILFGN
                     LYSFYDVDYVTRIGREVMEREEFYQEISRHKRLVLILALNCYQHCLEHSSFYNANYFE
                     AYTEKIIDKGIKLYERNAFHYLKGFALYQKGQCKEGCKQMQEAIHIFDVLGLPEQVAY
                     YQEHYEKFVKS"
     gene            complement(8568..10469)
                     /locus_tag="VVE42_001373"
     CDS             complement(8568..10469)
                     /locus_tag="VVE42_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU3073560.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVGEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFNNLRRTERQLR
                     QMELEMGEKSGEDLDKLMSDYDRLSENFRQAGGFTYEADIRAILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETIAWLENYLVNYSGALLIVSHD
                     RYFLDKVATITLDLTKHSLDRYVGNYSRFVELKEQKLATEAKNYEKQQKEIAALEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKAANMTFQSEKTSGNVVLTVENTAI
                     GYDGEVLSQPINLDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRVAT
                     HVLELSENGSTLYLGDYDYYVEKKATAEMSQTEEASTSNQAKEASPVNDYQAQKESQK
                     EVRKLMRQIESLEAEIEELESQSQAISEQMLETNDADKLMELQAELDKISHRQEEAML
                     EWEELSDQV"
BASE COUNT         3195 a         2353 c         1910 g         3209 t
ORIGIN      
        1 aaaccaggaa aacaatttcc tggttttaca cgttatttca catcaaaaac aatggatttg
       61 acatttgtca tcgcttcaat gctatattta attccttgca ctccagctcc agaacctttg
      121 acaccaagga atgggaaatt atctggacca cgctgggttt tattattaat gtggactgta
      181 cctacttcaa gtttttcagc aatttcaaag gcttttttga aatcatttgt aaagactgat
      241 gattgaaggc cgaattcaga ttcgttggca aaggcaatag cttcctctac actagccaca
      301 cgaatgattg gtaaaacagg accaaatggc tcttcccatg ccactttcat atcttttgta
      361 acttggtcaa aaagcactgg ccagagaaga ttgccctcac gtttgattgg tgtaagagcc
      421 tgagcttctt tttcttgtgc atcctcaatc aagctccaaa tgaagtcggc tgaagcattg
      481 tcaataacag gtgtaatatc agcattgtca aatggatcac caactgttaa tttagaaact
      541 tcttcctgaa gcaaagtagc taatttatct gctacacttt cgagaacaat gacacgttta
      601 atggccgtgc aacgttgtcc tgagtagcta aaggctcccg caacaatttg cttggcagca
      661 tgttccaaat ctgcatcttc tagtacaaga gctgcatctt tcccaccaag ttccaacatg
      721 ataggacgca taccagctaa acgaccaata cgttctccaa taggagttga acctgtaaag
      781 ttgatgaagt tgacttcttt gtgctcaatg atataatccc caatttctga accacgacct
      841 gtaatggtgt tgaaaacacc tgccggaatc cctgcttctt caaatgcttt agccaacaag
      901 agtccagaaa tggaaccttg tgttggtggc ttaaacatga ccacattccc tgcaatcaag
      961 gcaggtgcaa ttttagaagc agataaatta actggataat taaagggagc aatcgctagc
     1021 acgataccaa ctggttcacg acagacaaca gccagtttgt ttttacttgc tgcctcaaaa
     1081 ccaccacctt ccattgcttg tccagtgata cggagacctt cctcagcagc ataacgaatc
     1141 aagtctgctg tacgcactac ttctccaatt gctgctttaa tcccttttgc tacttctttg
     1201 gcaaggatag taccaatttt ttccttatcg cgttctaaaa tagctgctgt tttatgcaaa
     1261 taagccgcac gttcaattgc tgataaagct cgccatgctg gcagggctgc acgcgcagct
     1321 tgcatagcct catcagcttc agtctgagtc atggctggaa ctgtacccaa ttcttcttga
     1381 ttgattggtg aataaatcgt aatttcttgt tcagatgatt tccattttcc atttactaaa
     1441 ttctgatatc ttgtcaaatt cttgtcctcc tgaaaatgat taagatatat tttaccaaat
     1501 tttcagaaaa tcacaaccct ttttgcaaaa tttttgagat tattttcaca agcttgattt
     1561 ttcaaagtat actcaataaa aataaaaaaa atccactaca tcaaggcgag gctaatgtgg
     1621 tttgaagaaa ttttcgaaga gcgtgaatga gtatcatcta tagtaaaata aaaaaactga
     1681 acaatttggt tggggacagc caaaccaatt tctcacaatg tttcagaaac aagggtgtgc
     1741 tattccaatt tcagcctact ataactgtca tagattgctg aaacaaagtc taggtaaaag
     1801 tcttcataat aaaaagacct cctatcaagt gttcaaaaac tttgatagga ggtcttgttt
     1861 tgtgaaaata tttatcaaat tttctataca agtgagctgt tagccaggtt ctttctattc
     1921 tttcaatttc aatgaatgga ttttttatca atactcataa ctgggaattt gtctgtgtaa
     1981 aaatagcgag atagatggta tttataaaac actcaagaca gctagactaa tatcatttaa
     2041 aacattatct tcttttgagc gactgttggt taccaacata gctaaatttc ctgcattttc
     2101 aaattgatag ggttctgatt tagcattcac aaccaccaag aggtgttctt tgccgtgaac
     2161 ttcatagata aggcagccgc tatgttcaat cgcagaatgc acaaagacat gatggtaaat
     2221 ttcatcatag ctagagtaag aaaaggcact agtttttgtc ttcaatcgga tgacttgacg
     2281 gataaactca atactgtctt gacgctcatt aatcaagttc cagttcactt ggttcacact
     2341 gtcaggagca ttatagctat tcatcgcacg ctctctatca tcatgggtca actcaccatt
     2401 ttcaccagtc gcaaccagtt tggtacgacc aaattcttga ccgatttcca taaaggccat
     2461 cccctgcatg agcagattca tggctgtggc agtttcgatc ttgcgcatga tttgctctga
     2521 actttggtct ggatgaaggg ttgccaataa atcgtgaaga ttgtaattgt catgggcttc
     2581 tacatagtta agcacctgat ttggatgtgt ataggttcct aattcacgac ttcctaggat
     2641 tgctttagct agaattggct ctgtcgcagc accactgaca aaacctgact tgatagcacc
     2701 ataaacttct ccccctttga cagcatcgcg ctgattgtca ttaaagaaac caatatttgg
     2761 catctggtag gcattgtcct tcttggcctt atcataaggg gtaagacctg ttcccatatc
     2821 ccatccttct ccatagagga taatgttgga gtcgatttca tccaagcttt gacgaatcat
     2881 ctgcatggtc ttgacatcat gaatccccat caagtcaaaa cggaagccgt caatattata
     2941 ttcctgcacc cagtatagaa gagaatcaat catatacttg cgaaacattt cgtgttcact
     3001 ggctgtttca tttccaacac ccgttccatt ctggaaggta ccatctggat tcatacgata
     3061 atagtaatca gggactgttg tttggaatgg tgcatcaaca actgagaagg tatggttata
     3121 gactacatcc ataatgactc caatacccgc atcgtgataa gcttgaacca tcaccttcaa
     3181 atcacgaatg acctgagctg gatcatctgg attagttgaa aaactagttt ctggcgcgtt
     3241 atagttttgt ggatcataac cccagttgta ggttacattt ccatcctcat cgtattcttt
     3301 atgacggtct gcaattggtt gcaattgaac ataattgtag cccagcttct tgatgtaatc
     3361 aaaagcagtt gactggccgt attggttaac tgttccagcc tgagcagcac ccaagaaagt
     3421 tcctcgaaga tgttcatcta cacccgatgt agatgattta gtcaaatcac gaatatgcat
     3481 ttcacagata actgccttac atggattttc caagcgccaa gtagcctccg aaccgtgctt
     3541 aacctcgaag ttttcaactt gcttttctac atggctcaga atagctgaac gtttgccatc
     3601 agggctggtc gcgattgtat aaggatcacg tgtcagtgtt tggtgatgag ggaattggac
     3661 ttgatactga taagccttac ctaccaaatc ttcctcaaca tccaaactcc agacaccgat
     3721 tgtattgtcc ttatgattat aagagtagct attgcctctt ttcatctcaa aagtcttcca
     3781 aacgggtgca tcattagcag ctgattcata aacgacaact tgcacttctg tcgctgtagg
     3841 tgaccagagg gaaaaatgag cctgattgtc ctctacacgg caacccaatt ctccttggta
     3901 accccaatga tgatcaaaac tagcactgtt aatggcctta tcaaaggcaa aaggattttg
     3961 atttttatag aaaggactgg caatagcagg attttcagag taataaatcc tatcatcgcc
     4021 ttccaaaatc cagacctctg ttaatagggg atagtgatta aaacggatag aatattcttt
     4081 actagtttga cctgtatgaa ccacaaaatt caagctttct ataacatgtg aacttgggtg
     4141 ttcaaagcta aataaagctc caaaataatc ttctttgtag gttagcaaat caattcgttg
     4201 atcctgactt tttacaaagg agcaagtgtc atattctcca ttcttacgat ggtaatgaat
     4261 gcgcataggg tagttataca ttttttattt ttccttttta ctttgtttct atttcactaa
     4321 taaatttttg tcaatctcgt ctcaattaac agacatagtc atattctcta aactctgttt
     4381 ttaaacgatc cattacaaac tttctagcca tgcctcatct ctgacctgga taccaagttc
     4441 ttgtgctttt tgcagcttac ttccagcgtc tgcacctacc acgacgaggt tggtcttttt
     4501 agaaacacta cctgtcactt tggcacccag actttcgagt ttacttttag cttctgagcg
     4561 cttgagtcgt tccaattttc ctgtcaatac cacggtcaaa cctgacaagg ccgcatccgc
     4621 tactaccgtc tgtcctttat agtccagatt gaccccagtt tctttcaatt ctctgagcag
     4681 aatttcagag ccttctgtcg caaaataagt ctgaagactt ttggcaatca cgccacctag
     4741 actttcaata ctagccactt cctctgaatc tgcctgatac agattttcaa ttgaatggaa
     4801 atattgaagt aaaagctgac tagccttgct tccgacatga cgaattccca aaccaaataa
     4861 gagcttctcg gcagaatttt cctttgacgc ttggatagcc tgatacagtt tagcagcgga
     4921 cttttcctta accccctcta aaaggaggaa atcctcttct tgcaaacgat aaatatccgc
     4981 cacatccttg actaaattag cagcaaaaag cttctcaaca atagatggac caaggcctgt
     5041 aatattcata gcatcacgag aagcaaagtg aatcaagcct tccatgattt gagcagggca
     5101 acgcggattg atacaacgta gggccacttc atcttcaaag tgcaacaagt cagagttaca
     5161 acttggacag tttgtaggga tatctagttt ttcttcagaa acccgtttgg actctaccac
     5221 acgtaaaacg gcagggatga tgtcaccagc cttatataca atgaccgtat cgtcttttcg
     5281 gatatctttt tcagcaatat aatctacatt gtgcagggtc gcacggctaa cagtcgtacc
     5341 ggcaagttgt actggtgtta gattagcagt tggagttaca acaccggtac ggccaactgt
     5401 ccagtcaact gataagagtt gagcttcttt ttcttcagca gggaacttgt aggctactgc
     5461 ccactttgga gccttaacgg taaaaccaag ttcttcttga cttgctaggt cgttgacctt
     5521 gattaccact ccatcaatat cgtaaggtag attttcccgt tcctgtccta cttcttggat
     5581 aaaattccag atttcatcta tgttttcagc caagattcgc ttaggattga ccacaaaacc
     5641 tagttgttct aggtacttca aacccttttc ttggctatca cgagttgaag ggctggcttc
     5701 ttgatagaga aacgttgcaa gattacgctt ggcaactact gctgtatcca actgacgcag
     5761 agttcctgct gccgcattac gaggattagc aaattcaggc tctccatttt cttggcgcgc
     5821 ttggttaact tggtcaaagg aagcgcgtgg catgtaacat tccccacgaa ctgtgatatc
     5881 tagttcttct ggcaaagtca aagggatgtc cttaacacgc ttgaggtttt ctgtgatatt
     5941 ttcaccaatt gaaccatctc cacgtgttac cccagcaacc aaaatcccct tttcataagt
     6001 cagcgagata gataagccat cgattttcag ctcacaaata taggtcggat gagccacttc
     6061 cttacgaaca cgcgcatcaa aagcatctag ctcctcacgt gaaaaagcat cctgcaaact
     6121 ataaagagga tactgatgac tgtatttttc aaaaccatct aaaaccttgc caccaacacg
     6181 atgagtcgga ctgtctgcta gcacttgctc tggataagca gtttctaact cgaccaactc
     6241 acggtaaagg cggtcatact cactgtctga aaccgaggga ttatcgctgg tatagtactc
     6301 agtcgcatag cgattgagca aagcgactaa ctcattcatt cttttattca taagaccatt
     6361 ttaccataaa acaagccctc ctcacaaacg agaaggacgg aaaaaacact tagtttgaaa
     6421 ttatttttga aactcaagca accttatatc aatttttcaa aatgagttcg aacataaata
     6481 aacgatatac aagacaagat gataacacca cttccaatta tcaggaaaga agagagatgt
     6541 acacttggca agactgtcat aaatcctttt gcaataggca taaatagaat agctaaggta
     6601 aaaattgtac tcagtactct cccaagaaat tcgctctcaa ccttggtttg tacttgagta
     6661 aaaaagtgaa tattaaaaat cgtcataaac aattcacaaa ctaaatttcc agaaaaggaa
     6721 agaaaagttg gaagtggtaa tcccatcata aaaactccga cacctgtcaa agccagtaaa
     6781 atcaaaagat tataaatatt agctttaatt ttactagcta gaagagcccc aatgatggaa
     6841 ccaatagccc ccatagttaa aatacttgca taggctcctt ctgatccgta aagctgattc
     6901 gaaaagggaa gtagaaattc aaaagctgca aaaaagaaat taacgctgga agctaccagc
     6961 aaaaggaaga aaatttcttg ctgatgccag atatagtgta acccatcctt gatatctaca
     7021 aaaatatctc tcccagtaaa agcctttttc tcttgaactt ttgcttcctc ttttggaagg
     7081 aaagccacta gaacaaaagc aatgaaaaaa gtcagcgagt ctagcagtag cgtcatatgg
     7141 agacttgcaa actgtaaaac aaggaaggaa agaacaggag agctaacacc tacaacctgc
     7201 aaaaccagct ctaggcgaga attatagatc acaatctcat ctttctccac cacttcagtt
     7261 atgatagctt tattggctgt gcgagaaaag gcaaaagcaa tagcctgcac aatgttagca
     7321 acaatcaaag cgccaatcat ccagctatca ttccttatga aagaaatagc cagacaaaga
     7381 atcccacaaa caagatctgc cgtcattaaa atcttacgac gagaaaaacg gtctgaaata
     7441 actccgccaa agggattgac gagaatagat gtgacgagct cagaaatctg atacattcct
     7501 aaaactgtct gtcctatagt ccccatagaa gccaaccaga cactatttcc ataatcatag
     7561 agcatatttc ccattttatt gatagcccca cggctaatca actgcactgc atagcgattc
     7621 atattaaagc tcctctcaaa ttttgaaact attgtatcaa aaccgaaagg agctttttat
     7681 tttttccctt atttgggaaa attaactttt gacaaatttt tcgtagtgtt cctgataata
     7741 ggctacttgc tctggaagac ctaacacatc aaaaatatgt atggcctctt gcatctgctt
     7801 acagccttct ttacactgtc ctttttgata taaggcaaaa ccttttaaat aatggaaagc
     7861 attacgctca taaagcttaa tacctttgtc aataatcttc tctgtataag cctcaaaata
     7921 gttggcatta taaaaagaag aatgctctaa acaatgctgg taacaattga gggccaaaat
     7981 caaaactaat ctcttatggc gactaatctc ttggtaaaat tcctccctct ccataacttc
     8041 tctaccaatc cgagtgacat agtctacatc gtagaaacta tagaggttac cgaaaagaat
     8101 caactcatac atggtccatt cttctgtttt gaagagataa tctgctacct tatccaaatc
     8161 atcctgcttc atatcataac tcgcatctct ttgacaaatc agaccttgta gcaaaatcca
     8221 gttcagctca aaataaaggg gagtcgtcga actcttagac ttttcaagtt gttctctttg
     8281 aagcttttga aaacctgcaa tatcgtttga atagtaaagt gggataatct gtgccatcat
     8341 agacacatgt tcatgattat gaaaattcct tgccttatcc atgaaatttt cgattgttac
     8401 atgaatgtta tccaaaatct caaagaaacg ggagactgcc aggtcagact ccccaagctc
     8461 aaagcgagat aactgagagg tagagcagga ttcgcctgct gcttccttta aagaataatt
     8521 tccacttgtt cgaaattcac gaaatacttt tccaagatgt tccatcttta cacctgatct
     8581 gataattctt cccactcaag catagcttct tcctgacgat ggctgatttt gtccagctca
     8641 gcctgtaatt ccatgagttt gtcggcatcg tttgtttcca acatttgttc agaaatggct
     8701 tggctttgac tttctagctc ttcaatttca gcttctagac tttcgatttg tcgcatgagt
     8761 ttgcgaactt ctttttgact ttctttctgg gcctgatagt cattgactgg acttgcttcc
     8821 tttgcttgat tgctagttga agcttcctca gtctgactca tttctgctgt tgctttcttc
     8881 tcaacatagt agtcgtaatc tccaaggtag agagttgaac cattctcaga caattccaaa
     8941 acatgagttg ccacacgatt gataaagtaa cgatcatgac tgacaaaaag caaggtacca
     9001 tcaaagtcaa tcaaggcatt ttctagcact tccttactat caatatccaa gtggttggtc
     9061 ggctcatcca gaatcaaaaa gttattgttt tccatagaca atttagctaa aagcaaacga
     9121 gctttttcgc caccagatag catgccgact gattttttaa catcatctcc tgagaaaagg
     9181 aaggctccaa gacggttgcg gatttcaact tctggtgtca gtttgaaatc attccagagt
     9241 tcatccagca ccgtattact tggtgtcagc ttgctttggg tttggtcata gtaacccacc
     9301 tcaacattag cgccaaagcg cttttctccc ttgataaaag gaatctggtc cacaatagac
     9361 ttgataaagg ttgacttgcc gataccattt ggaccaacga tagcgacagc attcatctta
     9421 cgaagatcta ggttgatagg ttgtgacaag acttccccgt cataaccaat agcagtattt
     9481 tcaacagtca aaacaacatt gcccgacgtt ttttcagact ggaaggtcat gttggctgct
     9541 ttcttgccag cttcaggctt gtccaaacgc tccatttttt ctagttgttt acggcgagat
     9601 tgagcacgtt tagtcgttga agcacgaact agattgcgat tgacaaagtc ttccagagca
     9661 gcgatttcct tctgttgctt ttcatagttt tttgcctcag tagctagctt ttgctccttc
     9721 aattcgacaa aacgagagta attccccaca tagcgatcca aggaatgctt ggtcaaatct
     9781 agcgtaattg tcgcaacctt gtccaagaaa taacggtcgt ggctgacgat aaggagggca
     9841 ccgctatagt ttaccaagta attctctagc caggcgatgg tttcaatatc caagtggtta
     9901 gttggctcgt ccaagaccaa gagattgggc ttttcaagga gcattttggc aagtgccaaa
     9961 cgagtatttt gaccaccaga aagctcagca attttcatct gccacataga ctcgtcaaac
    10021 ttgaatccat tcaaaatcgc tcgaatatca gcttcatagg taaagccacc tgcttggcga
    10081 aaattctcag ataagcggtc ataatctgac atcagtttat ccaaatcctc accagacttt
    10141 tcacccatct ccagctccat ctgacgcagt tgtctctccg tccgacgcaa attattaaag
    10201 acatgaagca tttcatcgta gatggtattt tcagactcaa aacggctatc ttgggctagg
    10261 taagacagag aaatatcttt tttcttattg atttctccgc tagttggctc ctcttctcca
    10321 actaaaatct tcaaaagagt agacttacct gcaccatttt tcccaacaag ggcaatccga
    10381 tctcgttcat caacctgcag gttgatatta tcgaaaagaa cctctcctgc aaaagaacgt
    10441 tcaattttat tagcttgtaa aataatcata agagtagtat agcatgtttc cctaaggcat
    10501 tcaagataat cgtaagtctt ttagtacaac ttttataaca taaaataaac taaattatgt
    10561 atattttata ttagattctt cactatcttg ttggattttc taaccagcta atcttgtttc
    10621 aaatagttat cgcacaagcc tattatttaa ttcttttcat tatttac
//
LOCUS       DAVGJL010000069        10497 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.069, whole genome shotgun sequence.
ACCESSION   DAVGJL010000069 DAVGJL010000000
VERSION     DAVGJL010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10497
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.069"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            207..2867
                     /gene="rrgA"
                     /locus_tag="VVE42_001374"
     CDS             207..2867
                     /gene="rrgA"
                     /locus_tag="VVE42_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus tip adhesin RrgA"
                     /protein_id="HEU3073561.1"
                     /translation="MKKVRKIFQKAVAGLCCISQLTAFSSIVALAETPETSPAIGKVV
                     IKETGEGGALLGDAVFELKNNTDGTTVSQRTEAQTGEAIFSNIKPGTYTLTEAQPPVG
                     YKPSTKQWTVEVEKNGRTTVQGEQVENREEALSDQYPQTGTYPDVQTPYQIIKVDGSE
                     KNGQHKALNPNPYERVIPEGTLSKRIYQVNNLDDNQYGIELTVSGKTVYERKDKSVPL
                     DVVILLDNSNSMSNIRNKNARRAERAGEATRSLIDKITSDPENRVALVTYASTIFDGT
                     EFTVEKGVADKNGKRLNDSLFWNYDQTSFTTNTKDYSYLKLTNDKNDIVELKNKVPTE
                     AEDHDGNRLMYQFGATFTQKALMKADEILTQQARQNSQKVIFHITDGVPTMSYPINFN
                     HATFAPSYQNQLNAFFSKSPNKDGILLSDFITQATSGEHTIVRGDGQSYQMFTDKTVY
                     EKGAPAAFPVKPEKYSEMKAVGYAVIGDPINGGYIWLNWRESILAYPFNSNTAKITNH
                     GAPTRWYYNGNIAPDGYDVFTVGIGINGDPGTDEATATSFMQSISSKPENYTNVTDTT
                     KILEQLNRYFHTIVTEKKSIENGTITDPMGELIDLQLGTDGRFDPADYTLTANDGSRL
                     ENGQAVGGPQNDGGLLKNAKVFYDTTEKRIRVTGLYLGTGEKVTLTYNVRLNDQFVSN
                     KFYDTNGRTTLHPKEVEKNTVRDFPIPKIRDVRKYPAITIAKEKKLGEIEFIKINKND
                     KKPLRDAVFSLQKQHPDYPDIYGAIDQNGTYQNVRTGEDGKLTFKNLSDGKYRLFENS
                     EPAGYKPVQNKPIVAFQIVNGEVRDVTSIVPQDIPAGYEFTNDKHYITNEPIPPKREY
                     PRTGGIGMLPFYLIGCMMMGGVLLYTRKHP"
     gene            3090..5087
                     /gene="rrgB"
                     /locus_tag="VVE42_001375"
     CDS             3090..5087
                     /gene="rrgB"
                     /locus_tag="VVE42_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus major pilin RrgB"
                     /protein_id="HEU3073562.1"
                     /translation="MKSINKFLTMLAALLLTASSLFSAATVFAAGTTTTSVTVHKLLA
                     TDGDMDKIANELETGNYAGNKVGVLPANAKEIAGVMFVWTNTNNEIIDENGQTLGVNI
                     DPQTFKLSGAMPATAMKKLTEAEGAKFNTANLPAAKYKIYEIHSLSTYVGEDGATLTG
                     SKAVPIEIELPLNDVVDAHVYPKNTEAKPKIDKDFKGKANPDTPRVDKDTPVNHQVGD
                     VVEYEIVTKIPALANYATANWSDRMTEGLAFNKGTVKVTVDDVALEAGDYALTEVATG
                     FDLKLTDAGLAKVNDQNAEKTVKITYSATLNDKAIVEVPESNDVTFNYGNNPDHGNTP
                     KPNKPNENGDLTLTKTWVDATGAPIPAGAEATFDLVNAQTGKVVQTVTLTTDKNTVTV
                     NGLDKNTEYKFVERSIKGYSADYQEITTAGEIAVKNWKDENPKPLDPTEPKVVTYGKK
                     FVKVNDKDNRLAGAEFVIANADNAGQYLARKADKVSQEEKQLVVTTKDALDRAVAAYN
                     ALTAQQQTQQEKEKVDKAQAAYNAAVIAANNAFEWVADKDNENVVKLVSDAQGRFEIT
                     GLLAGTYYLEETKQPAGYALLTSRQKFEVTATSYSATGQGIEYTAGSGKDDATKVVNK
                     KITIPQTGGIGTIIFAVAGAVIMGIAVYAYVKNNKDEDQLA"
     gene            5134..6315
                     /gene="rrgC"
                     /locus_tag="VVE42_001376"
     CDS             5134..6315
                     /gene="rrgC"
                     /locus_tag="VVE42_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus minor pilin RrgC"
                     /protein_id="HEU3073563.1"
                     /translation="MISRIFFVMALCFSLVWGAHAVQAQEDHTLVLQLENYQEVVSQL
                     PSRDGHRLQVWKLDDSYSYDNRVQIVRDLHSWDENKLSSFKKTSFEMTFLENQIEVSH
                     IPNGLYYVRSIIQTDAVSYPAEFLFEMTDQTVEPLVIVAKKADTVTTKVKLIKVDQDH
                     NRLEGVGFKLVSVARDGSEKEVPLIGEYRYSSSGQVGRTLYTDKNGEIVVTNLPLGTY
                     RFKEVEPLAGYTVTTMDTDVQLVDHQLVTITVVNQKLPRGNVDFMKVDGRTNTSLQGA
                     MFKVMKEENGHYTPVLQNGKEVVVASGKDGRFRVEGLEYGTYYLWELQAPTGYVQLTS
                     PVSFTIGKDTRKELVTVVKNNKRPRIDVPDTGEETLYILMLVAILLFGSGYYLTKKTN
                     N"
     gene            6654..7550
                     /gene="srtC-1"
                     /locus_tag="VVE42_001377"
     CDS             6654..7550
                     /gene="srtC-1"
                     /locus_tag="VVE42_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus system sortase SrtC-1"
                     /protein_id="HEU3073564.1"
                     /translation="MAKTKKQKRNNLLLGVVFFIGMAVMAYPLVSRLYYRVESNQQIA
                     DFDKEKATLDEADIDERMKLAQAFNDSLNNVVSGDPWSEEMKKKGRAEYARMLEIHER
                     MGHVEIPAIDVDLPVYAGTAEEVLQQGAGHLEGTSLPIGGNSTHAVITAHTGLPTAKM
                     FTDLTKLKVGDKFYVHNIKEVMAYQVDQVKVIEPTNFDDLLIVPGHDYVTLLTCTPYM
                     INTHRLLVRGHRIPYVAEVEEEFIAANKLSHLYRYLFYVAVGLIVILLWIIRRLRKKK
                     RQSERALKALKEATKEVKVEDE"
     gene            7543..8436
                     /gene="srtC-2"
                     /locus_tag="VVE42_001378"
     CDS             7543..8436
                     /gene="srtC-2"
                     /locus_tag="VVE42_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus system sortase SrtC-2"
                     /protein_id="HEU3073565.1"
                     /translation="MSKSRYSRKKSVKKKKNPFILLLIFLVGLAVAMYPLVSRYYYRI
                     ESNEVIKEFDETVSQMDKAELEERWRLAQAFNATLKPSEILDPFTDQEKKQGVSEYAN
                     MLKVHERIGYVEIPAIEQEIPMYVGTSEDILQKGAGLLEGASLPVGGENTHTVITAHR
                     GLPTAELFSQLDKMKKGDIFYLHVLDQVLAYQVDQIVTVEPNDFEPVLIQHGQDYATL
                     LTCTPYMINSHRLLVRGKRIPYTAPIAERNRAVRERGQFWLWLLLGAMAVILLLLYRV
                     YRNRRIVKGLEKQLEGRHVKD"
     gene            8423..9273
                     /gene="srtC-3"
                     /locus_tag="VVE42_001379"
                     /pseudo
     CDS             8423..9273
                     /gene="srtC-3"
                     /locus_tag="VVE42_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091736.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus system sortase SrtC-3"
     gene            complement(9345..10497)
                     /locus_tag="VVE42_001380"
                     /pseudo
     CDS             complement(9345..10497)
                     /locus_tag="VVE42_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3369 a         1745 c         2444 g         2939 t
ORIGIN      
        1 caagtgtgtc attgccaggt tgagaagata gctataacgc acttttatac gcttttgcta
       61 cgtttgttag tgaacggatt aactcagtga gataaatttt atcagaacat aagtaatccg
      121 tttcttcgtg tatacagatt gaaagtacct atgaatcata gaaggattaa cttgttctat
      181 gaataatgct taacagggag acacacatga aaaaagtaag aaagatattt cagaaggcag
      241 ttgcaggact gtgctgtata tctcagttga cagctttttc ttcgatagtt gctttagcag
      301 aaacgcctga aaccagtcca gcgataggaa aagtagtgat taaggagaca ggcgaaggag
      361 gagcgcttct aggagatgcc gtctttgagt taaaaaacaa tacggatggc acaactgttt
      421 cgcaaaggac agaggcgcaa acaggagaag cgatattttc aaacataaaa cctgggacat
      481 acaccttgac agaagcccaa cctccagttg gttataaacc ctctactaaa caatggactg
      541 ttgaagttga gaagaatggt cggacgactg tccaaggtga acaggtagaa aatcgagaag
      601 aggctctatc tgaccagtat ccacaaacag ggacttatcc agatgttcaa acaccttatc
      661 agattattaa ggtagatggt tcggaaaaaa acggacagca caaggcgttg aatccgaatc
      721 catatgaacg tgtgattcca gaaggtacac tttcaaagag aatttatcaa gtgaataatt
      781 tggatgataa ccaatatgga atcgaattga cggttagtgg gaaaacagtg tatgaacgaa
      841 aagataagtc tgtgccgctg gatgtcgtta tcttgctcga taactcaaat agtatgagta
      901 acattcgaaa caagaatgct cgacgtgcgg aaagagctgg tgaggcgaca cgttctctta
      961 ttgataaaat tacatctgat ccagaaaata gggtagcgct tgtgacttat gcttccacta
     1021 tctttgatgg gaccgagttt acagtagaaa aaggggtagc agataaaaac ggaaaacgat
     1081 tgaatgattc tcttttttgg aattatgatc agacgagttt tacaaccaat accaaagatt
     1141 atagttattt aaagctgact aatgataaga atgacattgt agaattaaaa aataaggtac
     1201 ctaccgaggc agaagatcat gatggaaata gattgatgta ccaattcggt gccactttta
     1261 ctcagaaagc tttgatgaag gcagatgaga ttttgacaca acaagcgaga caaaatagtc
     1321 aaaaagtcat tttccatatt acggatggtg tcccaactat gtcgtatccg attaatttta
     1381 atcatgctac gtttgctcca tcatatcaaa atcaactaaa tgcatttttt agtaaatctc
     1441 ctaataaaga tggaatacta ttaagtgatt ttattacgca agcaactagt ggagaacata
     1501 caattgtacg cggagatggg caaagttacc agatgtttac agataagaca gtttatgaaa
     1561 aaggtgctcc tgcagctttc ccagttaaac ctgaaaaata ttctgaaatg aaggcggttg
     1621 gttatgcagt tataggcgat ccaattaatg gtggatatat ttggcttaat tggagagaga
     1681 gtattctggc ttatccgttt aattctaata ctgctaaaat taccaatcat ggtgccccta
     1741 caagatggta ctataacggg aatattgctc ctgatgggta tgatgtcttt acggtaggta
     1801 ttggtattaa cggagatcct ggtacggatg aagcaacggc tactagtttt atgcaaagta
     1861 tttctagtaa acctgaaaac tataccaatg ttactgacac gacaaaaata ttggaacagt
     1921 tgaatcgtta tttccacacc atcgtaactg aaaagaaatc aattgagaat ggtacgatta
     1981 cagatccgat gggtgagtta attgatttgc aattgggcac agatggaaga tttgatccag
     2041 cagattacac tttaactgca aacgatggta gtcgcttgga gaatggacaa gctgtaggtg
     2101 gtccacaaaa tgatggtggc ttgctaaaaa atgcaaaagt gttctatgat acgactgaga
     2161 aaaggattcg tgtaacaggt ttgtaccttg gaacgggtga aaaagttaca ttgacttata
     2221 atgttcgctt gaatgaccaa tttgtaagca ataaattcta tgacacgaat ggtcgaacaa
     2281 ccctacaccc taaggaagta gaaaagaaca cagtgcgcga cttcccgatt cctaagattc
     2341 gtgatgtgcg aaaatatcca gcaattacga ttgcaaaaga gaaaaaactt ggtgaaattg
     2401 agtttattaa gatcaataag aatgataaaa aaccactgag agatgcggtc tttagtcttc
     2461 aaaaacaaca tccggattat ccagatattt atggagctat tgatcaaaat ggcacttatc
     2521 aaaatgtgag aacaggtgaa gatggtaagt tgacctttaa aaatctgtca gatgggaaat
     2581 atcgattatt tgaaaattct gaaccagctg gttataaacc cgttcaaaat aagcctatcg
     2641 ttgccttcca aatagtaaat ggagaagtca gagatgtgac ttcaatcgtt ccacaagata
     2701 taccagcggg ttacgagttt acgaatgata agcactatat taccaatgaa cctattcctc
     2761 caaagagaga atatcctcga actggtggta tcggaatgtt gccattctat ctgataggtt
     2821 gcatgatgat gggaggagtt ctattataca cacggaaaca tccgtaaagt gtagaaatga
     2881 taatatctat gttctgaaca atacttttaa gaagtagcac tcaagaagag atttaagttt
     2941 acttggtgaa accagttttc ttcgccaagt aaaccaccat tgaaagggga gatgttttcg
     3001 aaaacttgca cagaaaaagg attattattg tcatgtgtaa ttcattacat tgctcacagt
     3061 tgattttaag agataaataa ggagaaatca tgaaatcaat caacaaattt ttaacaatgc
     3121 ttgctgcctt attattgaca gcgagtagcc tgttttcagc tgcaacagtt tttgcggctg
     3181 ggacgacaac aacatctgtt accgttcata aactattggc aacagatggg gatatggata
     3241 aaattgcaaa tgagttagaa acaggtaact atgctggtaa taaagtgggt gttctacctg
     3301 caaatgcaaa agaaattgcc ggtgttatgt tcgtttggac aaatactaat aatgaaatta
     3361 ttgatgaaaa tggccaaact ctaggagtga atattgatcc acaaacattt aaactctcag
     3421 gggcaatgcc ggcaactgca atgaaaaaat taacagaagc tgaaggagct aaatttaaca
     3481 cggcaaattt accagctgct aagtataaaa tttatgaaat tcacagttta tcaacttatg
     3541 tcggtgaaga tggagcaacc ttaacaggtt ctaaagcagt tccaattgaa attgaattac
     3601 cattgaacga tgttgtggat gcgcatgtgt atccaaaaaa tacagaagca aagccaaaaa
     3661 ttgataaaga tttcaaaggt aaagcaaatc cagatacacc acgtgtagat aaagatacac
     3721 ctgtgaacca ccaagttgga gatgttgtag agtacgaaat tgttacaaaa attccagcac
     3781 ttgctaatta tgcaacagca aactggagcg atagaatgac tgaaggtttg gcattcaaca
     3841 aaggtacagt gaaagtaact gttgatgatg ttgcacttga agcaggtgat tatgctctaa
     3901 cagaagtagc aactggtttt gatttgaaat taacagatgc tggtttagct aaagtgaatg
     3961 accaaaacgc tgaaaaaact gtgaaaatca cttattcggc aacattgaat gacaaagcaa
     4021 ttgtagaagt accagaatct aatgatgtaa catttaacta tggtaataat ccagatcacg
     4081 ggaatactcc aaagccgaat aagccaaatg aaaacggcga tttgacattg accaagacat
     4141 gggttgatgc tacaggtgca ccaattccgg ctggagctga agcaacgttc gatttggtta
     4201 atgctcagac tggtaaagtt gtacaaactg taactttgac aacagacaaa aatacagtta
     4261 ctgttaacgg attggataaa aatacagaat ataaattcgt tgaacgtagt ataaaagggt
     4321 attcagcaga ttatcaagaa atcactacag ctggagaaat tgctgtcaag aactggaaag
     4381 acgaaaatcc aaaaccactt gatccaacag agccaaaagt tgttacatat ggtaaaaagt
     4441 ttgtcaaagt taatgataaa gataatcgtt tagctggggc agaatttgta attgcaaatg
     4501 ctgataatgc tggtcaatat ttagcacgta aagcagataa agtgagtcaa gaagagaagc
     4561 agttggttgt tacaacaaag gatgctttag atagagcagt tgctgcttat aacgctctta
     4621 ctgcacaaca acaaactcag caagaaaaag agaaagttga caaagctcaa gctgcttata
     4681 atgctgctgt gattgctgcc aacaatgcat ttgaatgggt ggcagataag gacaatgaaa
     4741 atgttgtgaa attagtttct gatgcacaag gtcgctttga aattacaggc cttcttgcag
     4801 gtacatatta cttagaagaa acaaaacagc ctgctggtta tgcattacta actagccgtc
     4861 agaaatttga agtcactgca acttcttatt cagcgactgg acaaggcatt gagtatactg
     4921 ctggttcagg taaagatgac gctacaaaag tagtcaacaa aaaaatcacg atcccacaaa
     4981 cgggtggtat tggtacaatt atctttgctg tagcaggggc tgtgattatg ggtattgcag
     5041 tgtacgcata tgttaaaaac aacaaagatg aggatcaact tgcttaagta agagagaaag
     5101 gagccattga tgacaatgca gaaaatgcag aaaatgatta gtcgtatctt ctttgttatg
     5161 gctctgtgtt tttctcttgt atggggtgca catgcagtcc aagcgcaaga agatcacacg
     5221 ttggtcttgc aattggagaa ctatcaggag gtggttagtc aattgccatc tcgtgatggt
     5281 catcggttgc aggtatggaa gttggatgat tcgtattcct atgataatcg ggtgcaaatt
     5341 gtgagagact tgcattcgtg ggatgagaat aaactttctt ctttcaaaaa gacttcgttt
     5401 gagatgacct tccttgagaa tcagattgaa gtatctcata ttccaaatgg tctttactat
     5461 gttcgctcta ttatccagac ggatgcggtt tcttatccag ctgaatttct ttttgaaatg
     5521 acagatcaga cggtagagcc tttggtcatt gtagcgaaaa aagcagatac ggtgacaaca
     5581 aaggtgaagc tgataaaggt ggatcaagac cacaatcgct tggagggtgt cggctttaaa
     5641 ttggtatcag tagcaagaga tggttctgaa aaagaggttc ccttgattgg agaataccgt
     5701 tacagttctt ctggtcaagt agggagaact ctctatactg ataaaaatgg agagattgtt
     5761 gtgacaaatc ttcctcttgg gacctatcgt ttcaaggagg tggagccact ggcaggctat
     5821 actgttacga cgatggatac ggatgtccag ttggtagatc atcagctggt gacgattacg
     5881 gttgtcaatc agaaattacc acgtggcaat gttgacttta tgaaggtgga tggtaggacc
     5941 aatacctctc ttcaaggggc aatgttcaaa gtcatgaaag aagaaaacgg acactatact
     6001 cctgttcttc aaaatggtaa ggaagtagtt gtggcatcag ggaaagatgg tcgtttccga
     6061 gtggaaggtc tagagtatgg gacatactat ttatgggagc tccaagctcc aactggttat
     6121 gttcaattaa catcgcctgt ttcctttaca atcgggaaag atactcgtaa ggaactggta
     6181 acagtggtta aaaataacaa gcgaccacgg attgatgtgc cagatacagg ggaagaaacc
     6241 ttgtatatct tgatgcttgt tgccattttg ttgtttggta gtggctatta tcttacgaaa
     6301 aaaacaaata actgatattc aatgtacatc attatgaaaa agatagcagg ctgaagggaa
     6361 gaccagagta ctctgaggtg atgttaatca ggaatcatgg tgatttggca tgaatcataa
     6421 taacggatat gaggctgggc agattgtgcc agcctcattg tgggttattg tttgtaaaac
     6481 gataggactg gtctggtaat cattttagga atggacagga ctgggattct gatttaaaat
     6541 ggatggtgaa tcagaaagaa atgagatttt ctcgtttctc ttagcagata ggattgtctg
     6601 ttaggaaaag cgataaaatg atgagtttga agttaaagga atgctgataa aaaatggcaa
     6661 aaacaaaaaa gcaaaaacga aacaatctcc tattaggagt ggtatttttc attggaatgg
     6721 cggtaatggc gtatccgctg gtgtctcgct tgtattatcg agtggaatca aatcaacaaa
     6781 ttgctgactt tgataaggaa aaagcaacgt tggatgaggc tgacattgat gaacgaatga
     6841 aattggcaca agccttcaat gactctttga ataatgtagt gagtggcgat ccttggtcgg
     6901 aagaaatgaa gaaaaaaggg cgagcagagt atgcacgcat gttagaaatc catgagcgga
     6961 tggggcatgt ggaaatccct gctattgatg tagatttgcc ggtttatgct ggtactgctg
     7021 aagaggtatt gcagcaaggc gctggacatc tagagggaac ttctctaccg atcggaggca
     7081 attcgaccca tgcggtgatt acggcacata caggtttgcc aacggctaag atgtttacgg
     7141 atttgaccaa acttaaagtt ggggataagt tttatgtgca caatatcaag gaagtgatgg
     7201 cctatcaagt ggatcaagta aaggtgattg agccgacgaa ctttgatgat ttattgattg
     7261 taccaggtca tgattatgtg accttgctga cctgtacgcc atacatgatc aatacccatc
     7321 gtctattggt tcgggggcat cggataccgt acgtagcaga ggttgaggaa gaatttattg
     7381 cggcaaacaa actcagtcat ctctatcgct acctgtttta tgtggcagtt ggtttgattg
     7441 tgattctttt atggattatt cgacgcttgc gcaagaagaa acggcaatca gaaagagctt
     7501 tgaaagcatt gaaggaagct actaaggaag tgaaggtaga ggatgagtaa gagtagatat
     7561 tcacggaaaa agagcgtgaa aaagaagaaa aatccgttca ttcttcttct gatttttttg
     7621 gtggggcttg ccgttgcgat gtatcccttg gtgtctcgtt attattatcg tattgagtca
     7681 aacgaggtta ttaaagagtt tgatgagacg gtttcccaga tggataaggc ggaacttgag
     7741 gagcgttggc gcttggctca agccttcaat gcgaccttga aaccatctga aatcctcgat
     7801 ccttttacag atcaggaaaa gaaacaggga gtttcagaat atgctaacat gctaaaagtt
     7861 catgagcgta tcggatatgt agaaattcct gcgattgaac aggaaatccc catgtatgtt
     7921 ggcacaagtg aagacattct tcagaaaggg gcagggctgt tagaaggggc ttcgctgcct
     7981 gttggaggtg aaaataccca tacagtgatc actgctcaca gaggattgcc aacggcagaa
     8041 ttgttcagtc aattggataa gatgaagaaa ggggatatct tttatcttca cgttttagac
     8101 caggttttgg cctatcaagt ggatcagata gtgacggtgg agccgaatga ttttgagcct
     8161 gttctgattc aacatggaca agattatgcg actttactga cttgtacgcc atacatgatt
     8221 aacagccacc gtttgttggt acgtgggaaa cggattccat atacagcgcc gattgctgag
     8281 cggaatcgag cggtgagaga gcgtgggcaa ttctggttgt ggttattact aggagcgatg
     8341 gcggtcatcc ttctcttgct gtatcgcgtg tatcgtaatc gacggattgt caaaggacta
     8401 gaaaagcaat tggaggggcg tcatgtcaag gactaaacta cgagccttat tgggatactt
     8461 gttgatgttg gtagcctgtt tgattcctat ttattgtttt ggacagatgg tgttgcagtc
     8521 tcttggacag gtgaaaggtc atgctacatt tgtgaaatcc atgacaactg aaatgtacca
     8581 agaacaacag aaccattctc tcgcctacaa tcaacgcttg gcttcgcaaa atcgcattgt
     8641 agatcctttt ttggcggagg gatatgaggt caattaccaa gtgtctgacg accctgatgc
     8701 agtctatggt tacttgtcta ttccaagttt ggaaatcatg gagccggttt atttgggagc
     8761 agattatcat catttaggga tgggcttggc tcatgtggat ggtacaccgc tgcctctgga
     8821 tggtacaggg attcgctcag tgattgctgg gcaccgtgca gagccaagcc atgtcttttt
     8881 ccgccatttg gatcagctaa aagttggaga tgctctttat tatgataatg gccaggaaat
     8941 tgtagaatat cagatgatgg acacagagat tattttaccg tcggaatggg aaaaattaga
     9001 atcggttagc tctaaaaata tcatgacctt gataacctgc gatccgattc ctacctttaa
     9061 taacgcttat tagtgaattt tgaacgagtc gctgtttatc aaaaatcaga tccacaaaca
     9121 gctgcagttg cgagggttgc ttttacgaaa gaaggacaat ctgtatcgcg tgttgcaacc
     9181 tctcaatggt tgtaccgtgg gctagtggta ctggcatttc tgggaatcct gtttgttttg
     9241 tggaagctag cacgtttact acgagggaaa taaaaagaaa tgaaaggaaa gctaaggctg
     9301 ttcctttttc cggctctttg tcaactgtag tgggttgaag tcagctaagc tcgagaaagg
     9361 acaaatttcg tcctttcttt tttgatgttc aaagcgataa aaatccgttt tttgaagttt
     9421 tcaaagtttc gaaaaccaaa ggcattgcgc ttgataagtt tgatgagatt attggtcgct
     9481 tccagtttgg cattagaata gtgtagttga agggcgttga taatcttttc tttatctttg
     9541 aggaaggttt taaagacagt ctgaaaaata ggatgaacct gcttaagatt gtcctcgata
     9601 agttcgaaaa atttctctgg ttccttattc tggaagtgaa aaagcaagag ctgatagagc
     9661 tgatagtggt gtttcaagtc ttgtgaataa ctcaaaagct tgtttaaaat ctctttattg
     9721 gttaaatgca tacgaaaagt aggacgataa aatcgcttat cactcagttt acggctatcc
     9781 tgttgtatga gcttccagta gcgcttgata gccttgtatt catgggattt tcgatgaaac
     9841 tgattcatga tttgaacacg cacacgactc atagcacggc taagatgttg cacaatgtga
     9901 aagcgatcaa gaacgatttt agcattcggg agtgaaacag tctgggagac tgtttcagcc
     9961 tgagcctaga aatttgaaag cgaagctgtt tagccaagtc atagtaaggg ctaaacatat
    10021 ccatagtaat gattttgacg cgacatcgga cggctctatc atatttaaga aagtgatctc
    10081 ggatgacagc ttgtgttcta ccttcaagaa cagtgatgat atcgagcttt tcaaaatctt
    10141 gcgcaataaa gctcatctcc atctcccgat tgaaacagtc actccccgga ctgtttcaac
    10201 gtcccaggac ataatctcag gaagacgcga aaaatcatgc tcaaagtgag aatcattgag
    10261 cttgcgaatg acagttgaag ttgaaatggc cagctgatga gcaatatcgg tcatagaaat
    10321 cttctcaatc aacttttgcg caattttttg gttgatgata cgagggattt ggtgattttt
    10381 cttgacgata gaagtttcag cgaccatcat ttttgaacag tgatagcact tgaaacggcg
    10441 ttttctaagg agaattctag aaggcatacc agttgtttcg aggtaaggga tcttaga
//
LOCUS       DAVGJL010000070        10450 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.070, whole genome shotgun sequence.
ACCESSION   DAVGJL010000070 DAVGJL010000000
VERSION     DAVGJL010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10450
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.070"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(<1..509)
                     /locus_tag="VVE42_001381"
     CDS             complement(<1..509)
                     /locus_tag="VVE42_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU3073566.1"
                     /translation="MKKVKLGEILSLKKGKKATVLAEQTTLSQRYIQIDDLRNNNNLK
                     FTESLNMTEALPDDILIAWDGANAGTVGYGLSGAVGSTITVLKKNERYKEKIISDYLG
                     VFLESKSQYLRDHSTGATIPHLNKNILLDLQLELLGIEEQENIICILNTIKRLITKRK
                     FQLDELNLLV"
     gene            complement(522..2015)
                     /locus_tag="VVE42_001382"
     CDS             complement(522..2015)
                     /locus_tag="VVE42_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEU3073567.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
     gene            complement(2523..3689)
                     /locus_tag="VVE42_001383"
     CDS             complement(2523..3689)
                     /locus_tag="VVE42_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU3073568.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFRVDNLENIDEVKRAVAFLFYKHGPIDRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKTEADVDQAVKEIGLPMIAKPDNGVGA
                     AATFKLETEDDINHFKQEWDHSTLYFFEKFVTSSEICTFDGLVDKDGKIVFSTTFDYA
                     YTPLDLMIYKMDNSYYVLKDMDPKLRKYGEAIVKEFGMKERFFHIEFFREGDDYITIE
                     YNNRPAGGFTIDVYNFAHSLDLYRGYAAIVAGEEFPASEFEPQYCLATSRRANAHYVY
                     SEENLLAKYSQQFKVKKIMPEAFAELQGDYFYMLTTPSRQEMDQMIADFGQRQE"
     gene            complement(3702..4441)
                     /locus_tag="VVE42_001384"
                     /pseudo
     CDS             complement(3702..4441)
                     /locus_tag="VVE42_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546805.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
     gene            complement(4463..5287)
                     /locus_tag="VVE42_001385"
     CDS             complement(4463..5287)
                     /locus_tag="VVE42_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU3073569.1"
                     /translation="MNQSYFYLKMKEHKLKVPYTGKERRVRILLPKDYEKDTDRSYPV
                     VYFHDGQNVFYSKESFIGYSWKIIPAIKRNPDISRMIVVAIDNDGMGRMNEYAAWKFQ
                     ESPIPEQQFGGKGVEYAEFVMEVVKPFIDETYRTKADCQHTAMIGSSLGGNITQFIGL
                     EYQDQIGCLGVFSSANWLHQEAFNRYIECQKLSPDQRIFIYVGTEEADDTDKTLMDGN
                     IKQAYIDSSLCYYHDLIAGGVHLDNLVLKVQSGAIHSEIPWSENLPDCLRFFAEKW"
     gene            complement(5423..6637)
                     /gene="thiI"
                     /locus_tag="VVE42_001386"
     CDS             complement(5423..6637)
                     /gene="thiI"
                     /locus_tag="VVE42_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEU3073570.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYPQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSAVQEIMQDIYKE
                     GMTFKISSKRSDHTFELDSRELNQTLGGAVFEAIPNVQAQMKSPDINLQVEIREEAAY
                     LSYETVRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAE
                     KDEVDDLIDNLL"
     gene            complement(6646..7788)
                     /locus_tag="VVE42_001387"
     CDS             complement(6646..7788)
                     /locus_tag="VVE42_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU3073571.1"
                     /translation="MIYFDNSATTRPYPEALETYMQVASKILGNPSSLHRLGDQATRI
                     LDASRQQIADLIGKKSDEIFFTSGGTEGDNWIIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKAQGFEVDFAPVDNKGFVDVEALEDLIRPDTTLVSIMAVNNEIGSVQPIEAI
                     SKLLADKPTISFHVDAVQALAKIPTEKYLTERVDFATFSGHKFHGIRGVGFIYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIRQALL
                     DYPDIFVFSDEEDFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGT
                     LIAMGVDKDKAQSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            complement(7948..8325)
                     /locus_tag="VVE42_001388"
     CDS             complement(7948..8325)
                     /locus_tag="VVE42_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073572.1"
                     /translation="MKHKIMKKIIVVVFAIPWLTFLSQVHADYVSKSSSLDGYSLVGL
                     NVLNVTFNASGYSDTYSGSVSNMYFTDWAWFPNTISNRSTWKSSIPYGQRANGTVHIG
                     VGVNSPWGAVNLWGGQNYFSLDF"
     gene            complement(8335..10419)
                     /locus_tag="VVE42_001389"
     CDS             complement(8335..10419)
                     /locus_tag="VVE42_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU3073573.1"
                     /translation="MFEIRNLSLSFAGRRLLEPTDLIFERGKVYTIYGKSGVGKSSLL
                     NKIGLLSATDPSVSYFFDGKEIDIENKKSVSVFIAEEVAFVFQGRNLINDLTVFENLR
                     LALNFYDLSPDEIESKIDTVLADLQISSLKHAYPEDLSGGEEQRVAIARALVTGKTLI
                     LADEPTSSLDKENREKVSALLIDLAHKYHKIVLIVSHDEAMIAIGDVQLHFKDHKLVG
                     NCLILNSDEGIKDSKKWSSCLSSSHTKLSVLKHRRPFLPRLLAFFISLVVAIAVTSIN
                     FQSLFADKYHQLVNNSLENGFLVINDSLHLQTTKVLDDFLSFDKEEITSISTSQNIQT
                     VNPYIEFLSLGMTFDNSKDYSQLQKNFQPTLTIDGQTRSLTRNFSIQPLYQTNTTQRQ
                     IEYFDRSNEKGIYLSEQFISDEKLPNIVSGTTVTLTFYIPISLYESSIEKEGNILKGD
                     GDLFVKVDKTYPVLGIVKKSYPFEYSPYGNTLFMNIAEMEELQNEAIQQHPMTKMALD
                     GFPLKSWMPSAIHVLLKDSGAIPEELSRIRAISSQITILSSYENYEEFNKGLTYIRQF
                     LMLLSLVLLILVIAVLSFVFFLLNRPRRFEVGILKSLGYSTQNIVWLFLKELIGYGKT
                     ISILASCLLVILSILAMQVLKLEISDVFKFYLTSVFTLIGLSVSVLIISGLLPIYTTC
                     RQTVVDTIRKNG"
BASE COUNT         3118 a         2191 c         1852 g         3289 t
ORIGIN      
        1 acgagcaagt ttagttcatc taactgaaat tttcttttag taataagcct tttaatcgta
       61 ttaagaatac agataatgtt ctcttgttct tcgataccta gcaattctaa ttgtaaatca
      121 agtaatatat tcttgtttaa atgaggaata gttgcacctg ttgaatgatc tcgtaaatac
      181 tgcgatttac tttccaaaaa gactcccaag taatctgata taattttttc tttgtatcgc
      241 tcattctttt ttaagaccgt aattgtacta ccaacagctc ccgataaccc atagccaact
      301 gttcctgcat tagcaccatc ccatgctatt agaatatcat ctgggagtgc ttcagtcata
      361 tttaaacttt cagtgaattt taaattatta ttatttctta aatcatctat ttgaatataa
      421 cgttggctta gagttgtttg ttcagcaagt acagtggctt tctttccttt ttttagagat
      481 aagatttccc ccaacttcac ttttttcata cagccacctc cctacttgag taatttttcc
      541 aattcagcca agccagcttg aatttctttt tctaaatcat tgattttctt taagatgact
      601 tctgttggtt catactcaac tttttcatac tcaatctctt tatatttatt gatagacaaa
      661 tcataatcat tttcctttat ctcagcaact ggaacaaaga aagattgatc cgttctctga
      721 cgttctgctt ctttttcaag atgatgaaag cgttcgataa tatctggaat atcattgtcg
      781 ctaatcggtt gtcgcttatc atccaaactt aaaccatccg ctttcatatc gtaaaaccag
      841 actttgtcag taccaccatt accagttttt gtaaagatga gaatggcagt tgaaactcca
      901 gcataaggct tgaacacacc actaggcatt gagattacag catcaagctt atgattctct
      961 acaatttcct gacgaattcc tttatgagct ttagacgaac caaaaaggac accatcaggt
     1021 acgataactg ctgctcgtcc acctggtttt aaagttcgca agaaaagaga aagaaagagt
     1081 aattctgttt ttttggtttt tacggttgca agaaggtcat tagaggttga attgtagtca
     1141 agtgagccct taaaaggagg atttgctaaa accaaagtat atttatcggc ttcttcatta
     1201 tcttgagaca gcgagtcaag gtaactgatt tgtggatttt ctactccatg tagcatcatg
     1261 ttcatcgccc caagtctcaa catagtcgta tccgtatcat ttccatgaaa catctgatta
     1321 tgaaaatgat tgatattatc tgtattggtt tcccattcat ctttcttacg ctttaagtaa
     1381 cggctagcag atactaagaa gccagcagaa cccatagcgg gatctgagat gatatctttg
     1441 atagtcggtt gcatcaactc aaccatcata tcgatgatgt gacgaggtgt acggaactgt
     1501 ccatttttac ctgcggtcga caattttgat aacagatatt catagatatc tccgatatca
     1561 gtaatacttt gtttgtcatt atcaaaatct acatctaatc ccctagttgg aaaaacatct
     1621 aagatagaaa ttgccttttg aagcgtagca ggtttattta tttgaaaaat agcttctcgc
     1681 atatatcgtg aaaaggctgt atcatctgta tcccccttga gatttttaat aaacggaaaa
     1741 atctcctgag tcattaaacg ataaacttcc tgagcatctc ctatattttt aaaagttgac
     1801 caacggtatt caggtttatc ttttggaaaa actccctcat aaggaatccc tagaaattca
     1861 gcatcacttt cacgtccaag ctcgacacta tccaaatctt tcataaataa gagataagtc
     1921 aactgttcaa tatttgttaa aggatttgcg tttccttctg tccaaagaat ttcccacagc
     1981 tgatcgattt tattttttaa ttcgcctgta atcatatctt cccttttctt tagtttttaa
     2041 aacttagtaa tatcattatt ttatcagatt tcaaacaaag cttcaattaa ctatatctat
     2101 gcgtcacctg aatattttat actcttcgaa aatctcttca aaccacgtca gcttcacctt
     2161 gccgtaggta tatgatactg actctgtcag ttctatccac aacctcaaaa cagtgttttg
     2221 agcaacctgc ggctagcttc ctagtttgct ctttgatttt cattgagtat taatagactt
     2281 gtttggtatc ctgaaaacta tatcgtatag gtgtcgtcat ggatcctttt agctgattta
     2341 aggatacgaa aacacaatac tatgactaaa ttgaaatgtt aaaattcaat ttctagccac
     2401 ctaatcctgc ttcaaatagt tatcgaacaa gttttatgat agctaccttg ttaggatgct
     2461 cttatttttt accagacaaa acaaaaggat taagggagtg aatttcctta atccgatagt
     2521 tcttattctt gacgttgtcc gaaatcagca atcatctgat ccatttcttg tcgactcgga
     2581 gttgtcagca tataaaagta atctccttga agttccgcaa aagcctctgg catgattttt
     2641 ttaaccttga actgctggct atatttggca agtaagttct cctcagaata gacatagtga
     2701 gcatttgcac gacgtgatgt agccaaacaa tactgaggtt caaactctga cgctgggaac
     2761 tcttctcctg cgacaatggc tgcatatcca cgatagaggt ccaaggaatg agcaaagtta
     2821 taaacatcaa tggtgaaacc acctgcaggg cggttattgt actcaatggt aatataatcg
     2881 tccccctcac ggaagaactc aatatggaaa aaccgttctt tcataccaaa ttctttgaca
     2941 attgcttccc catacttgcg cagtttagga tccatatcct tgagcacata ataagaattg
     3001 tccatcttat aaatcatgag gtcaagcggt gtataggcgt agtcaaaggt tgttgagaag
     3061 acaatctttc catccttgtc cacgagcccg tcaaaggtac agatttcgct ggaagtgaca
     3121 aatttttcaa agaaataaag ggttgaatgg tcccattctt gcttgaagtg attgatatcg
     3181 tcttctgtct caagtttaaa ggttgcggct gctcccactc cattatcagg tttggcaatc
     3241 attggaagac cgatttcttt cactgcttga tcaacatctg cttccgtctt gataacagct
     3301 ccaggtacca caggaacacc tgcttttttg aaaagtttct tcatttcaga cttatatttc
     3361 gtctttttga gatcctctgg tttggcacca aaaacattga attgttctct gagtgttgcg
     3421 tctagctcaa gccagtattc attgtgagac tcgatgcggt caattggacc atgtttataa
     3481 aagagaaaag caactgcacg tttgacttca tctatgttct caagattatc aacacgaaaa
     3541 tactcggtca agctattgcg caagggctca tccaattgct cgtaagactc ttgaccaatt
     3601 cccaagactg tgatgccttt attagctagt tcgatggtaa actgttgaaa gttttgtgga
     3661 tagtagggag aaataacaag gtaattcata ggtaactcct tttataaata gagattaccg
     3721 aggaaataag gcatttgttt acgccaccat tcccagtcat gggcgacatc atgtccccat
     3781 tcagcaaacc aggctggaat ttgtttcttg tcaaaggctt ctttgagctt gtaaaaggat
     3841 ggcaaaccat cttgttccca ggctccaagc cccgtacaca gcacaatctc tgcctgacgg
     3901 taacggtcaa taaaccagcc gtcgttttgg ttccaaatat aatctactgg cgagttttgg
     3961 taaatagcat catcgttgta gtaatcaccg acaaagaaac gtgcgtcgta aacaccactg
     4021 agagcaatca ctttggtaaa gacatctgga tgctggagga agaaattgag tgcatgatag
     4081 gctcccatag agcaacctgt cgtcatcatg ccatcaaacc aacctgtctt gtgcttgata
     4141 aaagaatggc ctcctcaatc acataacatt cgtaggcacg gtgcatttcc gcttggtcat
     4201 gagcattttt ccaagtagcc aaccagctct cactatccaa actagatagg gtaaagaact
     4261 ggacacggcc ttcctcgata aaggaagcac aggcatcaat catgccaaaa tcatagtatt
     4321 cgttgtgact actacctgat gaagcaaaga ccacaactgg aatcccacca tgtccataac
     4381 ggttaaggta catttcacgg ttaggatgac cactccagtg gctaagatgt tcaatatgca
     4441 tttcgttttt tcctttctta acttaccatt tttctgcaaa aaatctcaga caatctggta
     4501 gattttctga ccaagggatt tcactatgga tggcaccaga ctgaactttt agcacaagat
     4561 tatccagatg tactccccct gctatcaaat catggtaata gcaaagcgac gagtcgatat
     4621 aggcttgttt gatattgcca tccatcaagg tcttgtctgt atcatctgct tcttctgttc
     4681 ctacatagat gaagatgcgc tggtcaggcg atagtttctg gcactcgata tagcggttaa
     4741 aggcttcttg gtggagccag tttgcagatg aaaaaacgcc caagcaacca atttggtctt
     4801 ggtattccaa accgataaac tgggtaatat tgcctcctag tgaggaacca atcatagccg
     4861 tatgctggca gtctgctttt gtacgatagg tctcatcgat aaaaggcttg accacctcca
     4921 tgacaaactc agcatactcc acacccttac caccaaactg ctgctctggg ataggagatt
     4981 cttggaactt ccaagccgca tactcattca ttcgccccat accatcattg tcaatagcaa
     5041 cgacaatcat gcgactgata tccggatttc gtttgatagc tgggataatc ttccatgaat
     5101 atccaatgaa agactctttg ctataaaaaa cattttgccc gtcatgaaag tatacaacag
     5161 gataggaacg gtctgtatct ttctcataat ctttaggaag aagaatacgt acacggcgct
     5221 ccttacctgt ataaggaacc ttgagtttgt gttctttcat ttttagataa aagtaggatt
     5281 gattcattgt cagaaaactc tctatattca aaattttata tcattatatc atgaaaatag
     5341 aaaaaagtta gttttttacc tgtttttaga aaaaaaacta acttttttta ctgattttcg
     5401 ctattctttt ggattttctg aattagagta gattatcaat caagtcatca acttcatctt
     5461 tttcggcttg aggtgtgatt tcagtaatca tgattccagc cactgctcgc tcaaccaagc
     5521 cttcaacatc catacgcgct tcgtactgct ccgcattctt aattttagga tttgtttttg
     5581 gacgatctgg tgcaaaaatg gtacaacagt cttcaaacgg ttggattgaa atgtcaaagg
     5641 tatcgatttc ctgggcgatg tcaatgattt ccaacttgtc catggtaacc acaggacgaa
     5701 tgatgggagt gttggtaaca gcattgatag ccttcatact ttcaagggtt tggctggcta
     5761 cttgacctag actttcccca ttgatgataa ccaaaccatt tcgtacctca cgaatacggt
     5821 cagtaatacg catcataaaa cgacgcgtca aggtcataag gtaggcttct ggcgctttgg
     5881 ctttgatttc ctcttgaatc tctgtaaaag gaacttcgat aaactggata tttcccccaa
     5941 atttggttaa tttacgggtc aaatcttggg cttttttaag agcaccagga ctcgtgtaag
     6001 gtgggctggc aaagtgaact gcctctatat ctacccctcg tttaagcgct agatagccag
     6061 ctacagggga atcgattccc cctgacaaca tgagcatgcc cttaccagaa gttcccactg
     6121 gtaatccacc tgcccctcga acggtttcat aagaaagata ggctgcttct tcacgtatct
     6181 ccacctgaag attgatgtca ggacttttca tttgagcttg tacgtttgga atggcttcga
     6241 atacagcccc tccaagcgtt tggttgagtt cacgactgtc caattcaaag gtgtggtcgc
     6301 tacgcttgct agagatttta aaggtcatgc cttccttgta gatgtcctgc ataatctctt
     6361 ggacagcaga cttcagaact tctacagatt tttcaacctt ataaacaggg gaaaagtttt
     6421 gaattccaaa tacttgtttg agcgattctg ctactgctgt gtaatctgct ccattgaggt
     6481 aagcgtgggc acggtcgcga tctgctgtta ccttaacttg gggatagata gacaaaacgt
     6541 ctgaaatatt attgcgaagt ttattgatga aacgcatacg atttttgccc ttggttgaca
     6601 gctcaccgta gcgaatcata atttctgaat actgcatgaa tgctcctatc ttacttttct
     6661 agtttgattg taaattaatt ttagcttggt caagaactgc tcgacctgac tcatatcatt
     6721 ttcaaggtct agactaagac gcacagctga ctgagcctta tccttgtcca ctcccatagc
     6781 aatcagggtt ccggctggtt tcccagcctt ggacgaacaa gccgaggtcg ttgagatgaa
     6841 aatatcatag tcttcaaagg cgtgaacaat gacctcacca cgaacaccct tgattccaaa
     6901 agtcagaata tgaggggcga agtcttcctc atctgaaaag acaaatatat ccggatagtc
     6961 cagaagtgct tgacgaatca ctgccttcat ctgcccagtc ttgctcctaa agatatctag
     7021 cttttccata gataaacgga gagccttagc ggtcgctgca attcctgcca cattttcagt
     7081 tgtcgaacgg taatcacgtt cctgaccacc acctgttaaa agaggcgtaa tcttcttgcc
     7141 agacttgata tagataaaac caacaccacg gattccgtgg aacttgtgac cagagaaagt
     7201 cgcgaaatcc actcgttctg tcagatactt ttcagtcgga atcttggcaa gcgcctgaac
     7261 cgcatcaacg tggaaggaaa tagttggctt gtctgccaat agttttgaaa tagcctcaat
     7321 gggctgaaca gagccaattt cattgttaac tgccatgatg gaaacgaggg tcgtatcagg
     7381 tcttatcaaa tcttctagcg cctcaacatc cacaaatcct ttgttatcaa ctggagcaaa
     7441 atccacttca aaaccttgag ctttcaacca gagggctgat tccttaactg ctggatgttc
     7501 aatggctgat acgatgatgt gcttgccaaa ctgggctttt tcaaatgcta ctcccttgat
     7561 gatccagtta tccccttctg tcccaccaga tgtaaagaag atttcatcac ttttcttacc
     7621 gattaaatct gcaatctgtt gacgggaagc atctaaaatt cgtgttgcct ggtctcccaa
     7681 acgatggaga ctagatggat ttcctaaaat ttttgaagcc acctgcatat aggtttcaag
     7741 ggcttctgga tagggcctgg tcgtcgccga attatcaaag taaatcatgt ttctcacgct
     7801 ttctaaaatc actccttcta ttgtatcatg aaacggagcc tgcgacaaga aagggcaatg
     7861 tttgcattaa tctgaataac ataaaatact cttgatacca tcatcaagag cattcttatt
     7921 cattctatct aactttgatt atttctgtta aaaatctaaa gaaaagtaat tttgtccgcc
     7981 ccagagatta accgctcccc aaggcgaatt tactcctaca cctatatgga ctgtaccatt
     8041 tgcgcgttgg ccatatggaa tcgaactttt ccaagtggaa cgattactta tcgtattagg
     8101 aaaccaagcc cagtcagtga aatacatatt tgacacactg ccactataag tatcagaata
     8161 gccagaagca ttaaaagtca cattaagaac attcaagcct accaagctat aaccgtctaa
     8221 agaactagac ttacttacat aatctgcatg tacttgactt aaaaatgtta accatgggat
     8281 agcaaacact accactatta tttttttcat tatcttatgt ttcatgatat cacctcatcc
     8341 attctttcgg atagtatcga caactgtttg cctacaagtt gtgtatatag gtagtaagcc
     8401 acttattatc agtactgaga ctgataatcc aattaaggtg aaaacggaag ttagatagaa
     8461 tttaaaaaca tctgaaattt ccaatttaag cacttgcatt gctaaaatgg aaagaatgac
     8521 gagtaaacaa cttgctaata tcgaaattgt tttaccataa ccaattaatt cttttaagaa
     8581 aagccaaaca atattttgag tactgtatcc caaagatttt aaaatcccta cttcaaagcg
     8641 tcgaggacga ttgagcaaaa agaaaacaaa ggacaatact gcgatgacaa ggatgagtaa
     8701 gactagggat agcaacatta aaaattgcct gatataggtg agtcctttat taaattcttc
     8761 gtagttttca tatgatgata aaatagtaat ttgtgaagag attgctctta ttctactcaa
     8821 ttcttcagga atagcaccgc tatcctttag taacacatga atcgcactcg gcatccatga
     8881 tttcaaagga aaaccatcta aagccatctt cgtcatggga tgctgttgaa ttgcttcatt
     8941 ttgcaattct tccatttcag caatattcat aaatagggtg ttcccataag gggaatattc
     9001 aaacggataa ctttttttaa caatccctaa aacaggatat gttttatcca ctttgacaaa
     9061 taaatcgcca tctcctttca atatattccc ctctttctct atacttgatt catataaaga
     9121 aattggaata taaaaagtca gtgtcacggt agttcccgaa actatatttg gtaatttttc
     9181 atctgaaatg aattgctcag atagataaat ccctttttca ttgctcctat caaaatactc
     9241 tatctgcctc tgtgttgtat ttgtctgata caagggttga attgaaaaat ttcttgtcaa
     9301 gcttctggtc tgaccatcaa tagtcaaagt tggctgaaag ttcttttgaa gttgtgaata
     9361 gtcttttgaa ttatcaaacg tcatacccaa agagagaaac tctatataag gattgacggt
     9421 ttgaatattc tgacttgtgc taattgaggt gatttcctcc ttatcaaaac tgagaaaatc
     9481 atccaacact tttgtagttt gtaaatgaag cgaatcatta atcaccaaga agccattttc
     9541 gagtgagttg ttcaccagtt ggtgatactt atctgcaaat aaggactgaa agttaataga
     9601 ggttactgct atagcaacta caagagagat aaaaaacgct aataatctag gtaaaaaagg
     9661 ccttctatgt ttcaaaacgg atagcttcgt atgagaagac gacaaacagg aggaccactt
     9721 cttagaatcc tttatccctt catcagaatt caaaataaga caattcccaa ctaatttatg
     9781 gtctttaaaa tgaagctgaa catctcctat tgcaatcatc gcctcatcat gcgaaacaat
     9841 caatacaatt ttatgatatt tgtgagctaa atcaattaac aaggcggaga ctttttctct
     9901 attttcttta tccaaagaac ttgttggttc atcagctaag atgagagtct ttcctgtaac
     9961 taaggctctt gcgattgcca ctcgttgttc ttcaccaccg gataaatctt ctggataagc
    10021 atgctttaaa gaagaaatct gtaaatccgc caatactgta tcaattttgc tttctatttc
    10081 atctggactc aagtcataaa agtttaaagc tagtcttaaa ttttcaaata cggtcaaatc
    10141 atttattaga tttcgcccct gaaatacaaa ggcaacttct tcagctataa agacagagac
    10201 tgatttctta ttttcgatat caatctcttt tccatcgaag aaataagaaa cactaggatc
    10261 cgtagcagag agcaaaccaa ttttattcag taaagaagac tttcctacgc ctgacttccc
    10321 atatattgta tatacttttc ccctttcaaa tatcaaatct gttggctcta gcaaccgcct
    10381 gcctgcaaaa gagagactca aatttctaat ttcgaacata taacgccctc cttttttgaa
    10441 atcgctttct
//
LOCUS       DAVGJL010000071        10346 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.071, whole genome shotgun sequence.
ACCESSION   DAVGJL010000071 DAVGJL010000000
VERSION     DAVGJL010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10346
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.071"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            143..5083
                     /locus_tag="VVE42_001390"
     CDS             143..5083
                     /locus_tag="VVE42_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEU3073574.1"
                     /translation="MKNPFFERRCRYSIRKLSVGACSLMIGAVLFAGPALAEETAVPE
                     NSGANTELVSGESEHSTNEADKQNEGEHTRENKLEKAEGVATASETAEAASAAKPEEK
                     ASEVVAETPSAEAKPKSDKETEAKPEATNQGDESKPAAEANKTEKEVQPDVPKNTEKT
                     LKPKEIKFNSWEELLKWEPGAREDDAINRGSVVLASRRTGHLVNEKASKEAKVQALSN
                     TNSKAKDHASVGGEEFKAYAFDYWQYLDSMVFWEGLVPTPDVIDAGHRNGVPVYGTLF
                     FNWSNSIADQERFAEALKQDADGSFPIARKLVDMAKYYGYDGYFINQETTGDLVKPLG
                     EKMRQFMLYSKEYAAKVNHPIKYSWYDAMTYNYGRYHQDGLGEYNYQFMQPEGDKVPA
                     DNFFANFNWDKAKNDYTIATANWIGRNPYDVFAGLELQQGGSYKTKVKWNDILDENGK
                     LRLSLGLFAPDTITSLGKTGEDYHKNEDIFFTGYQGDPTGQKPGDKDWYGIANLVADR
                     TPAVGNTFTTSFNTGHGKKWFVDGKVSKDSEWNYRSVSGVLPTWRWWQTSTGEKLRAE
                     YDFTDAYNGGNSLKFSGDVAGKTDQDVRLYSTKLEVTEKTKLRVAHKGGKGSKVYMAF
                     STTPDYKFDDADAWKELTLSDNWTNEEFDLSSLAGKTIYAVKLFFEHEGAVKDYQFNL
                     GQLTISDNHQEPQSPTSFSVVKQSLKNAQEAEAVVQFKGNKDADFYEVYEKDGDSWKL
                     LTGSSSTTIYLPKVSRSASAQGTTQELKVVAVGKNGVRSEAATTTFDWGMTVKDTSLP
                     KPLAENIVPGATVIDSTFPKTEGGEGIEGMLNGTITSLSDKWSSAQLSGSVDIRLTKP
                     RTVVRWVMDHAGAGGESVNDGLMNTKDFDLYYKDADGEWKLAKEVRGNKAHVTDITLD
                     KPITAQDWRLNVVTSDNGTPWKAIRIYNWKMYEKLDTESVNIPMAKAAARSLGNNKVQ
                     VGFADVPAGATITVYDNPNSQTPLATLKSEVGGDLASAPLDLTNQSGLLYYRTQLPGK
                     EISNVLAVSVPKDDRRIKSVSLETGPKKTSYAEGEDLDLRGGVLRVQYEGGTEDELIR
                     LTHAGVSVSGFDTHHKGEQNLTLQYLGQPVNANLSVTVTGQDEASPKTILGIEVSQEP
                     KKDYLVGDSLDLSEGRFAVAYSNDTMEEHSFTDEGVEISGYDAQKTGRQTLTLRYQGH
                     EVNFDVLVSPKAALNDEYLKQKLAEVEAAKNKVVYNFASPEVKEAFLKAIEAAEQVLK
                     DHETSTQDQVNDRLNKLTEAHKALNGQEKFKEEKTELDRLTGEVQELLAAKPNHPSGS
                     ALAPLLEKNKALVEKVDLSPEELATAKQSLKDLVALLKEDKPAVFSDSKTGVEVHFSN
                     KEKTVIKGLKVERVQASAEEKKYFAGEDAHVFEIEGLDEKGQDVDLSYASIVKIPIEK
                     DKKVKKVFFLPEGKEAVELAFEQTDSHVIFTAPHFTHYAFVYESAEKPQPAKPAPQNK
                     VLPKPTYQPASDQQKAPKLEVQEEKVAFHRQEHENTEMLVGEQRVIIQGRDGLLRHVF
                     EVDENGQRRLRSTEVIQEAIPEIVEIGTKVKTVPAVVATQEKPAQNTAVKSEEASKQL
                     PNTGTADANEALIAGLASLGLASLALTLRRKREDKD"
     gene            5173..6369
                     /locus_tag="VVE42_001391"
     CDS             5173..6369
                     /locus_tag="VVE42_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEU3073575.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            6505..7032
                     /locus_tag="VVE42_001392"
     CDS             6505..7032
                     /locus_tag="VVE42_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEU3073576.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFGFFGWKGLQI
                     GALTAVFSLRESFDESVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETISYVFVRVSETFMGVFVAIIVN
                     YDIDRIRLFLEKKEK"
     gene            7109..7465
                     /gene="glnR"
                     /locus_tag="VVE42_001393"
     CDS             7109..7465
                     /gene="glnR"
                     /locus_tag="VVE42_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HEU3073577.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYEDQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRRALHNEL
                     LQQGRFASVQSPFGRG"
     gene            7502..8848
                     /gene="glnA"
                     /locus_tag="VVE42_001394"
     CDS             7502..8848
                     /gene="glnA"
                     /locus_tag="VVE42_001394"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEU3073578.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYIAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY"
     gene            complement(8944..9063)
                     /locus_tag="VVE42_001395"
     CDS             complement(8944..9063)
                     /locus_tag="VVE42_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEU3073579.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKSNSQKSQEDFSKNK"
     gene            9551..10346
                     /locus_tag="VVE42_001396"
                     /pseudo
     CDS             9551..10346
                     /locus_tag="VVE42_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187211.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT         3380 a         1845 c         2234 g         2887 t
ORIGIN      
        1 tagatttgtg aaaacagcta ttgttttttg tctaaaaatg ttttaggata aaaatgtgat
       61 tatcatgatt ctagagttaa aaaactacag aatataaagc gctttcttga aaacaacaaa
      121 atcaatcttt taggaggaga aaatgaagaa tccatttttt gaaagacgtt gtcgttacag
      181 tattcgtaag ttatcagtag gagcctgctc gctgatgatt ggtgctgttt tatttgctgg
      241 tccagccttg gctgaagaaa ctgcagttcc tgaaaatagc ggagctaata cagagcttgt
      301 ttcaggagag agtgagcatt cgaccaatga agctgataag cagaatgaag gggaacatac
      361 tagagaaaac aagctagaaa aggcagaagg agtagcgaca gcatctgaaa ctgcagaagc
      421 agctagcgca gctaaaccag aggaaaaagc aagtgaggtg gttgcagaaa caccatctgc
      481 agaagcaaaa cctaagtctg acaaggaaac agaagcaaag cccgaagcaa ctaaccaagg
      541 ggatgagtct aagccagcag ccgaagctaa taagactgaa aaagaagtcc agccagatgt
      601 ccctaaaaat acagaaaaaa cattaaaacc aaaggaaatc aaatttaatt cttgggaaga
      661 attgttaaaa tgggaaccag gtgctcgtga agatgatgct attaaccgcg gatctgttgt
      721 cctcgcttca cgtcggacag gtcatttagt caatgaaaaa gctagcaagg aagcaaaagt
      781 tcaagcctta tcaaacacca attctaaagc aaaagaccat gcttctgttg gtggagaaga
      841 gttcaaggcc tatgcttttg actattggca atatctagat tcaatggtct tctgggaagg
      901 tctcgtacca actcctgacg ttattgatgc aggtcaccgt aacggggttc ctgtatacgg
      961 tacactcttc ttcaactggt ctaatagtat tgcagatcaa gaaagatttg ctgaagcttt
     1021 gaagcaagac gcagatggta gcttcccaat tgcccgtaaa ttggtagaca tggccaagta
     1081 ttatggctat gatggctatt tcatcaacca agaaacaact ggagatttgg ttaaacctct
     1141 tggagaaaag atgcgccagt ttatgctcta tagcaaggaa tatgctgcta aggtaaacca
     1201 tccaatcaag tattcttggt acgatgccat gacctataac tatggacgtt accatcaaga
     1261 tggtttggga gaatacaact accaattcat gcaaccagaa ggagataagg ttccggcaga
     1321 taacttcttt gctaacttta actgggataa ggctaaaaat gattacacta ttgcaactgc
     1381 caactggatt ggtcgtaatc cttatgatgt atttgcaggt ttggaattgc aacagggtgg
     1441 ttcctacaag acaaaggtta agtggaatga cattttagac gaaaatggga aattgcgcct
     1501 ttctcttggt ttatttgccc cagataccat tacaagttta ggaaaaactg gtgaagatta
     1561 tcataaaaat gaagatatct tctttacagg ttatcaagga gaccccactg gccaaaaacc
     1621 aggtgacaaa gattggtatg gtattgctaa cctagttgcg gaccgtacgc cagcggtagg
     1681 taatactttt actacttctt ttaatacagg tcatggtaaa aaatggttcg tagatggtaa
     1741 ggtttctaag gattctgagt ggaattatcg ttcagtatca ggtgttcttc caacatggcg
     1801 ctggtggcag acttcaacag gggaaaaact tcgtgcagaa tatgatttta cagatgccta
     1861 taatggcgga aattccctta aattctctgg tgatgtagcc ggtaagacag atcaggatgt
     1921 gagactttat tctactaagt tagaagtaac tgagaagacc aaacttcgtg ttgcccacaa
     1981 gggaggaaaa ggttctaaag tttatatggc attctctaca actccagact acaaattcga
     2041 tgatgcagat gcatggaaag agctaaccct ttctgacaac tggacaaatg aagaatttga
     2101 tcttagctca ctagcgggta aaaccatcta tgcagtcaaa ctatttttcg agcatgaagg
     2161 tgctgtaaaa gattatcagt ttaacctagg acaattaact atctcggaca atcaccaaga
     2221 gccacaatcg ccgacaagct tttctgtagt gaaacaatct cttaaaaatg cccaagaagc
     2281 ggaagcagtt gtgcaattta aaggcaacaa ggatgcagat ttctatgaag tttatgaaaa
     2341 agatggagac agctggaaat tactaactgg ctcatcttct acaactattt atctaccaaa
     2401 agttagccgc tcagcaagtg ctcagggtac aactcaagaa ctgaaggttg tagcagtcgg
     2461 taaaaatgga gttcgttcag aagctgcaac cacaaccttt gattggggta tgactgtaaa
     2521 agataccagc ctaccaaaac cactagctga aaatatcgtt ccaggtgcaa cagttattga
     2581 tagtactttc cctaagactg aaggtggaga aggtattgaa ggtatgttga acggtaccat
     2641 tactagcttg tcagataaat ggtcttcagc tcagttgagt ggtagtgtgg atattcgttt
     2701 gaccaagcca cgtaccgttg ttagatgggt catggatcat gcaggagctg gtggtgagtc
     2761 tgttaacgat ggcttgatga acactaaaga ctttgacctt tattataaag atgcagatgg
     2821 tgagtggaag ctagctaagg aagtccgtgg taacaaagca cacgtgacag atatcactct
     2881 tgataaacca atcactgctc aagactggcg cttgaatgtt gtcacttctg acaatggaac
     2941 tccatggaag gctattcgta tctataactg gaaaatgtat gaaaagcttg atactgagag
     3001 tgtcaatatt ccgatggcca aggctgcagc ccgttctcta ggcaataaca aggtacaagt
     3061 tggctttgca gatgtaccgg ctggagcaac tattaccgtt tatgataatc caaattctca
     3121 aactccgctc gcaaccttga agagcgaagt tggaggagac ctagcaagtg caccattgga
     3181 tttgacaaat caatctggtc ttctttatta tcgtacccag ttgccaggca aggaaattag
     3241 taatgtccta gcagtttccg ttccaaaaga tgacagaaga atcaagtcag tcagcctaga
     3301 aacaggacct aagaaaacaa gctacgccga aggggaggat ttggacctta gaggtggtgt
     3361 tcttcgagtt cagtatgaag gaggaactga ggacgaactc attcgcctaa ctcacgcagg
     3421 tgtatcagta tcaggttttg atacgcatca taagggagaa cagaatctta ctctccaata
     3481 tttgggacaa ccagtaaatg ctaatttgtc agtgactgtc actggccaag atgaagcaag
     3541 tccgaaaact attttgggaa ttgaagtaag tcaggaaccg aaaaaagatt acctagttgg
     3601 tgatagctta gacttgtctg aaggacgctt tgcagtggct tatagcaatg acaccatgga
     3661 agaacattcc tttactgatg agggagttga aatttctggt tacgatgctc aaaagactgg
     3721 tcgtcaaacc ttgacgcttc gttaccaagg tcatgaagtc aactttgatg ttttggtatc
     3781 tccaaaagca gcattgaacg atgagtacct caaacaaaaa ttagcagaag ttgaagctgc
     3841 taagaacaag gtggtctata actttgcttc accagaagta aaagaagctt tcttgaaagc
     3901 aattgaagcg gccgaacaag tgttgaaaga ccatgaaact agcacccaag atcaagtcaa
     3961 tgaccgactt aataaattga cagaagctca taaagctctg aatggtcaag agaaatttaa
     4021 ggaagaaaag acagagcttg atcgtttaac aggtgaggtt caagaactct tggctgccaa
     4081 accaaaccat ccttcaggtt ctgccctagc tccgcttctt gagaaaaaca aggccttggt
     4141 tgaaaaagta gatttgagtc cagaagagct tgcaacagcg aaacagagtc taaaagatct
     4201 ggttgcttta ttgaaagaag acaagccagc agtcttttct gatagtaaaa caggtgttga
     4261 agtacacttc tcaaataaag agaagactgt catcaagggt ttgaaagtag agcgtgttca
     4321 agcaagtgct gaagagaaga aatactttgc tggagaagat gctcatgtct ttgaaataga
     4381 aggtttggat gaaaaaggtc aagatgttga tctctcttac gcttctattg tgaaaatccc
     4441 aattgaaaaa gataagaaag ttaagaaagt atttttctta cctgaaggca aagaggcagt
     4501 agaattggct tttgaacaaa cggatagtca tgttatcttt acagcaccac actttactca
     4561 ttatgccttt gtttatgaat ctgctgaaaa accacaacct gctaaaccag caccacaaaa
     4621 caaagtcctt ccaaaaccta cttatcaacc ggcttctgat caacaaaagg ctcctaaatt
     4681 ggaagttcaa gaggaaaagg ttgcctttca tcgtcaagag catgaaaata ctgagatgct
     4741 agttggggaa caacgagtca tcatacaggg acgagatgga ctgttaagac atgtctttga
     4801 agttgatgaa aacggtcagc gtcgtcttcg ttcaacagaa gtcatccaag aagcgattcc
     4861 agaaattgtt gaaattggaa caaaagtaaa aacagtacca gcagtagtag ctacacagga
     4921 aaaaccagct caaaatacag cagttaaatc agaagaagca agcaaacaat tgccaaatac
     4981 aggaacagct gatgctaatg aagccctaat agcaggctta gccagccttg gtcttgctag
     5041 tttagccttg accttaagac ggaaaagaga agataaagat taaatatcga aaaatcttgt
     5101 gaaatctttc cttatatttc caaagtgtga tataatagtt tcgaataaaa taaataaagg
     5161 agtttttgta acatggcaaa acttactgtt aaagacgttg acttgaaagg taaaaaagtc
     5221 ctcgttcgtg ttgacttcaa cgtaccattg aaagatggcg taatcactaa cgataaccgt
     5281 atcacagcag ctcttccaac tattaagtac atcatcgaac aaggtggacg tgcaattctt
     5341 ttctctcacc ttggacgtgt gaaagaagaa gctgataaag ctggtaaatc acttgctcct
     5401 gtagcagctg acttggcagc aaaacttggt caagatgttg ttttcccagg tgtcactcgt
     5461 ggtgctgaat tggaagcggc aatcaacgct cttgaagatg gacaagttct cttggttgaa
     5521 aacactcgtt acgaagatgt tgacggcaag aaagaatcta aaaacgatcc tgaacttggt
     5581 aaatactggg catcacttgg agatggtatc ttcgtaaacg atgcattcgg tacagctcac
     5641 cgtgcacacg catctaacgt tggtatctca gcaaacgttg aaaaagcagt tgctggtttc
     5701 cttcttgaaa acgaaattgc ctacatccaa gaagcagttg aaactccaga acgtccattc
     5761 gtggctatcc ttggtggttc aaaagtttca gacaagatcg gtgttatcga aaacttgctt
     5821 gaaaaagctg ataaagtcct tatcggtggt gggatgactt acacattcta caaagcacaa
     5881 ggtatcgaaa tcggtaactc acttgtagaa gaagacaaat tggatgttgc gaaagctctt
     5941 cttgaaaaag caaatggtaa attgatcttg ccagttgact caaaagaagc taacgcattt
     6001 gctggttaca ctgaagtgcg tgacactgaa ggtgaagcag tttctgaagg cttccttggt
     6061 cttgacatcg gtccaaaatc tatcgccaaa tttgacgaag ctttgactgg tgccaaaaca
     6121 gttgtatgga acggacctat gggtgtattt gaaaacccag atttccaagc tggtacaatc
     6181 ggtgtgatgg acgctatcgt gaaacaacca ggagttaaat caatcatcgg tggtggtgac
     6241 tcagctgccg cagcgattaa ccttggccgt gcagacaagt tctcatggat tagtacgggt
     6301 ggtggagcat caatggaact tcttgaaggt aaggttcttc caggacttgc agccttgaca
     6361 gaaaaataag attttataaa taaatcaaag aagagaggga tgaaagttcc tcttttcttt
     6421 tgcttaaaat aaaaacgctt cctctcaact attactcata aaaatcaccg atttatgata
     6481 aaatggaaat agaaagttga gattatgagt tattttaaaa aatataaatt cgataaatcc
     6541 cagttcaaac ttggtatgag aacctttaaa acaggtattg ctgtttttct agttctcttg
     6601 atttttggct tttttggctg gaaaggtctt caaattggtg ctttgacagc cgtttttagc
     6661 ctgagggaga gttttgatga gagtgttcat tttgggactt cgcgtattct aggaaatagt
     6721 atcggtggac tctatgcctt ggtcttcttc ttattaaata cctttttcca tgaagccttt
     6781 tgggtgacct tggtagttgt tccaatctgc accatgttaa ccattatgac aaatgtagcc
     6841 atgaataaca aagcaggggt tattggtggt gtagcagcta tgttaatcat taccctatca
     6901 attccaagtg gtgagacaat ttcgtacgtg tttgtgcgtg tatcagaaac gtttatggga
     6961 gtttttgtcg caattatcgt aaattacgat attgatcgta ttcgtctctt tttagagaaa
     7021 aaagaaaaat aatgttacat tttataacat tatcaattga cgtttgtctt tttttagact
     7081 ataatagaca gaaagaagga aattgtaaat gaaggaaaaa gaatttcgcc gaaatatggc
     7141 tgtttttcct atcggcagtg ttatgaagtt gaccgatcta tcggcgcgtc agattcgtta
     7201 ttatgaagat caagagttga tcaagcccga tcgaaacgaa ggaaatcgtc gcatgtattc
     7261 cttgaatgac atggatcgtc tgcttgaaat caaagattat atctctgaag gttataatat
     7321 cgctgccatt aagaaaaaat atgctgaacg tgaagcgaaa tccaagaaag cggttagtca
     7381 gactgaagta cgtcgtgcac ttcacaatga actcctccaa caggggcgtt ttgcttcagt
     7441 acagtcacct tttggtcgcg gttaggcaac cgcaagtagt catagattaa ggagaaaact
     7501 tatgccaatc acagctgcag atattcgtcg tgaagtcaag gaaaaaaatg ttacctttat
     7561 tcgtcttatg ttctcagata ttttgggaac catgaaaaac gtcgaaattc ctgctacaga
     7621 tgaacagtta gataaggtct tgtcgaacaa ggttatgttt gatggatctt ctattgaagg
     7681 ttttgtacgt atcaatgagt cggatatgta cttgtacccg gacttggata catggacagt
     7741 cttcccttgg ggagatgaaa atggaagtgt tgcaggtctg atctgtgatg tctatacaac
     7801 agaaggtgaa ccatttgcgg gtgaccctcg tggtaatttg aaacgagctc ttcgtcacat
     7861 ggaagaagtt ggattcaaat ccttcaacct tggtccagag ccagaattct tcctatttaa
     7921 gttggatgaa aatggggacc caacacttga agtgaatgac aagggtggct actttgattt
     7981 ggcacctact gaccttgcgg acaacacacg tcgtgagatt gtgaatgtct tgaccaaaat
     8041 gggatttgaa gtagaagcga gtcaccacga ggttgcggtt ggacagcatg agattgactt
     8101 taagtacgat gaagttctcc gtgcttgtga taagattcaa atctttaagc ttgttgttaa
     8161 aaccattgct cgcaaacacg gactttacgc aacatttatg gcgaagccaa aatttggtat
     8221 tgctggatca ggtatgcact gtaatatgtc cttgtttgat gcagaaggaa ataacgcctt
     8281 ctttgatcca aatgatccaa aaggaatgca gttgtcagaa acagcttacc atttcctagg
     8341 cggtttgatc aagcatgctt acaactatac tgccatcatg aacccaacag ttaactcata
     8401 caaacgtttg gttccaggtt atgaagcgcc tgtttacatt gcttgggctg gtcgtaaccg
     8461 ttcgccactt gtgcgcgtac ctgcttcacg tggtatggga actcgtcttg agttgcgttc
     8521 agtggatcca atggcgaacc cttacattgc tatggctgtt cttttggaag ttggtttgta
     8581 tggtattgaa aataaaatcg aagcaccagc tcctatcgaa gaaaatatct acatcatgac
     8641 agcagaagag cgcaaggaag ctggtattac agaccttcca tcaactcttc ataatgcttt
     8701 gaaagctttg acagaagatg aagtggttaa agctgctctc ggagatcaca tctacactag
     8761 cttccttgaa gccaaacgaa tcgaatgggc aagttatgca accttcgttt cacaatggga
     8821 aattgataat tatttagacc tttactaata ctaatataga agaaagattg cctgtgggta
     8881 aacagtcaca cagcagtcta agccatcaaa tacctataac ccttaaaata gcgatttcgg
     8941 tcgctattta tttttcgaaa aatcctcttg actcttttgc gaattagatt tatcatcaga
     9001 cgtgtagaag aaaatcaagg agtcactatg gaaaaattca atttcaaaaa caatatcggg
     9061 caagaaaaca agttacttca aatcgaaatc tacaagttta ctaacttttg caagcttcaa
     9121 aattacacat ctgtaaatat tttttcgaaa gatatatttg aagccatagt aaattgagac
     9181 tagaatagta cactacatct gctaaaaatt tctagaaatt aatttaactt tcctaatcga
     9241 tttgtttaca tcttatttca atctactata ttatctcaat aaatgatgat ggcgactgtt
     9301 ttgtttgtgc ttgtcgattt ttgaatcctg atcgagcgga agaatatttc tgccgaggtt
     9361 gtcaagggga aaatcctgaa gatattgaat tttatgatga acaattacag gcagaaaagg
     9421 tggaggcttt gaatattcga ttggaagtaa aaaattgaat tatctacaat ctcatcaaca
     9481 ataaacctat ttcaacccca ttatctatag gcgtattttt acgccttttt atatacttga
     9541 agcacataag aaacattatg ggaagtttgc tgatggaagc actcaaggaa ttgatgttcc
     9601 ttatgatatt cctgatactt gggagtgggt gaggttttct acattggttg aaattgtcag
     9661 aggtggctct ccacgaccaa tcaaagatta tcttacttct gaagtagatg gaataaattg
     9721 gataaaaata ggtgatactg aaaagggtga aaagtatata aataatgtta aagaaaaaat
     9781 caaaaaatca gggcttaaca aaactagatt tgtaaaaaaa ggtacatttt tgttaactaa
     9841 ttctatgagt tttggtagac cttatatttt gaatgttgat ggtgcaatac acgatggatg
     9901 gttggctatt tcgaactatg aaaactcatt aaataaagat tacctattct atattctttc
     9961 atcaaatgta gtttattctc aatttctatc tctaattagt ggagctgttg tgaaaaactt
    10021 gaatagtgat aaagttgctt ctattcttat ccctctccct ccgctagctg aacaacaacg
    10081 aatagtagaa gcaatcgaat cagctttaga aaaagtagat gaatatgctg aaagttataa
    10141 tagactagaa cagctagata aagaatttcc agataaacta aaaaaatcta ttcttcaata
    10201 tgctatgcaa ggaaaattag ttgaacaaga cccaaatgat gaatcagtcg aagttttact
    10261 tgaaaaaata cgagcagaaa aacaaaaact ctttgaagaa ggcaagatta aaaagaaaga
    10321 tttggacatt tctattgttt cccaag
//
LOCUS       DAVGJL010000072        10301 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.072, whole genome shotgun sequence.
ACCESSION   DAVGJL010000072 DAVGJL010000000
VERSION     DAVGJL010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10301
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.072"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            301..993
                     /locus_tag="VVE42_001397"
     CDS             301..993
                     /locus_tag="VVE42_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU3073580.1"
                     /translation="MNTSFVHAADGIQYVRDDTRDKEEGIEYDDADNGDIIVKVATKP
                     KVVTKKISSTRIRYEKDETKDRSENPVTIDGEDGYVTTTRTYDVNPETGHVTEQVTVD
                     RKEATDTVIKVPAKSKVEEVLVPFATRYEADNDLSAGQEQEITLGKNGKTVTTITYDV
                     DGKSGQVTESTLSQKEDSQTRVVKKGTKPQVLVQEIPIETEYLDGPTLDKSQEVEEVG
                     EIGKLLLLQSIL"
     gene            1047..1436
                     /locus_tag="VVE42_001398"
     CDS             1047..1436
                     /locus_tag="VVE42_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU3073581.1"
                     /translation="MVKRRIRRGTREPEKVVVPEQSSIPSYPVSVTSNQGTDVAVEPA
                     KAVAPTTGWKQENGMWYFYNTDGSMATGWVQVNGSWYYLNSNGSMKVNQWFQVGGKWY
                     YVNTSGELAVNTSIDGYRVNDNGEWVR"
     gene            complement(2097..3125)
                     /gene="queA"
                     /locus_tag="VVE42_001399"
     CDS             complement(2097..3125)
                     /gene="queA"
                     /locus_tag="VVE42_001399"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEU3073582.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFH
                     SIIDMLEPGDALVMNDTRVLPARLYGQKVETGGHVELLLLKNTSGDEWEVLAKPAKRL
                     KVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSEEAAATLRSVKENGGRVIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHAIQEHYRFFS
                     FGDAMFIY"
     gene            3276..3983
                     /locus_tag="VVE42_001400"
     CDS             3276..3983
                     /locus_tag="VVE42_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEU3073583.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESDLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
                     FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLLEK"
     gene            4123..4299
                     /locus_tag="VVE42_001401"
     CDS             4123..4299
                     /locus_tag="VVE42_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEU3073584.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            4429..5364
                     /locus_tag="VVE42_001402"
     CDS             4429..5364
                     /locus_tag="VVE42_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEU3073585.1"
                     /translation="MSVLVKEVIEKLRLDIVYGEPELLEKEINTADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLPETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            5357..6145
                     /locus_tag="VVE42_001403"
     CDS             5357..6145
                     /locus_tag="VVE42_001403"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEU3073586.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGALVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGATVDNLDYLPGFIRDQMY
                     IEGSYRQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFGFRVSQWLSVVLIGLGIMIVIYQNRKKAPYYITEEEN"
     gene            6146..6529
                     /locus_tag="VVE42_001404"
     CDS             6146..6529
                     /locus_tag="VVE42_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEU3073587.1"
                     /translation="MLEVAYILVALALIVFLVYLIITVQKLGRVIDETEKTIKTLTSD
                     VDVTLHHTNELLAKVNVLADDINVKVATIDPLFSAVADLSLSVSDLNDHARVLSKKAS
                     SAGSKTLKTGASLSALRLASKFFKK"
     gene            6545..6934
                     /locus_tag="VVE42_001405"
     CDS             6545..6934
                     /locus_tag="VVE42_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEU3073588.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQICSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQAKSYAFQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIDITEE"
     gene            7018..8148
                     /locus_tag="VVE42_001406"
     CDS             7018..8148
                     /locus_tag="VVE42_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEU3073589.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            8153..8890
                     /locus_tag="VVE42_001407"
     CDS             8153..8890
                     /locus_tag="VVE42_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEU3073590.1"
                     /translation="MGLTYQMKMKIPFDMADMNGHIKLPDVILLSLQVSGMQSIELGV
                     SDKAILEDYNLVWIIAEYDIEVVRLPRFAEEITIETEALSYNRLFCYRRFTIYNEAGQ
                     ELIHMMATFVLMDRDSRKVHAVEPEIVAPYQSDFDKKLIRGPKYESLEEPISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSVVERTG
                     LESKHEITSDGVINAQAIITWQEIKKA"
     gene            8901..9674
                     /locus_tag="VVE42_001408"
     CDS             8901..9674
                     /locus_tag="VVE42_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEU3073591.1"
                     /translation="MKYKGYLIDLDGTIYKGKDRIPAGETFVHELQKRGIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVYVVGEAGLKEAIK
                     AAGYVEDKENPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     AGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     GIPTLLVTTGFTKAEEVAGLPIAPTHVVSSLAEWDFDEN"
     gene            9664..10281
                     /locus_tag="VVE42_001409"
     CDS             9664..10281
                     /locus_tag="VVE42_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEU3073592.1"
                     /translation="MKTKLTFWGSMLFLLSLSILLTIYLAWIFYPMEIQWLNLTNRVY
                     LKPETIQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLAQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLTLVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYESFFGYLCLKSRRK"
BASE COUNT         3085 a         1849 c         2334 g         3033 t
ORIGIN      
        1 aattatcaag ttggaatgtt tgtatctaaa tatattagca tgtatttaga taagatgtct
       61 gcaatccttt atatatgaga ttgattacga tatagttaaa gtgttagttc aataatcaat
      121 atatcattct aagtctattt gatatataaa gtatactgtt taacgtttga tagaaaagat
      181 gctatactag aagtaggatg taatggcaaa atctaatact aataagcgct atgcattacg
      241 taaactcgga aagatttttg gttctgcggt aatcgggcta actttatttt taggaggaat
      301 ctgaatacaa gttttgttca tgctgctgat gggattcaat atgtcagaga tgatactaga
      361 gataaagaag agggaataga gtatgatgac gctgacaatg gggatattat tgtaaaagta
      421 gcgactaaac ctaaggtagt aaccaagaaa atttcaagta cgcgaattcg ttatgaaaaa
      481 gatgaaacaa aagaccgtag tgaaaatcct gttacaattg atggagagga tggctatgta
      541 actacgacaa ggacctacga tgttaatcca gagactggtc atgttaccga acaggttact
      601 gttgatagaa aagaagccac ggatacagtt atcaaagttc cagctaaaag caaggttgaa
      661 gaagttcttg ttccatttgc tactagatat gaagcagaca atgacctttc tgcaggacag
      721 gagcaagaga ttactctagg aaagaatggg aaaacagtta caacgataac ttatgatgta
      781 gatggaaaga gtggacaagt aactgagagt actttaagtc aaaaagaaga ctctcaaaca
      841 agagttgtta aaaaaggaac caagccccaa gttcttgtcc aagaaattcc aattgaaaca
      901 gaatatctcg atggcccaac tcttgataaa agtcaagaag tagaagaagt aggagaaatt
      961 ggtaaattac tcttactaca atctatactg tagatgaacg tgatggaaca attgaagaaa
     1021 ctacttctcg tcaaattact aaagagatgg taaaaagacg tataaggaga gggacgagag
     1081 aacctgaaaa agttgttgtt cctgagcaat catctattcc ttcgtatcct gtatctgtta
     1141 catctaacca aggaacagat gtagcagtag aaccagctaa agcagttgct ccaacaacag
     1201 gctggaaaca agaaaatggt atgtggtatt tttataatac tgatggttcc atggcaacag
     1261 gttgggtaca agttaatggt tcatggtact acctcaacag caacggttct atgaaagtca
     1321 atcaatggtt ccaagttggt ggtaaatggt attatgtaaa tacatcgggt gagttagcgg
     1381 tcaatacaag tatagatggc tatagagtca atgataatgg tgaatgggtg cgttaacaga
     1441 ttcctccctg cgacaaaatt gataatagta tgtctgaata tataattata tataaaaagc
     1501 tatagataag gtcatcttat agcgactata aaaaagtgaa caagcctagt cttctgtaca
     1561 ttagaattca gatttgttca ctttttgttt aatactcaat gaaaatcaaa gagcaaacta
     1621 gaaaactagt cgcaggttgc tcaaagtact gctttgaggt tgtagataga actgacgaag
     1681 tcagtcacat atataatcca aggtgacgtt gacgcggttt gaagagattt ttgaagagta
     1741 taagtctttg attttgagac taaggagagt cagggtcaaa gtagtgaatg attctgaaat
     1801 aactcataat agtctcttga gaggacagat aagagtaaca aatatggtgc tactcatgtg
     1861 tcttgcattt tgatttacaa acagcgaaga aaatatagga agctgaaata aaatatagat
     1921 gaatcaattt ggagagtcaa cgcaatctat ggcaatattt tcgaagctag ggggaagcat
     1981 tttaatttca atgtactagg cgtcttctac agctagctag agtttcaatt gtagatgttc
     2041 tttgaaataa tatcttagcg atttattggt attagaagaa ttagagaaat tctttctcaa
     2101 taaataaaca tggcgtcacc aaaactgaag aagcggtagt gttcttggat ggcatggtgg
     2161 taggcatcta agactaattc acggcctgca aaggcagaaa ccaacatgac cagagttgat
     2221 tttggcagat ggaagttggt tgagaaggca tccacgacct tccactcata cccaggtttg
     2281 ataaagatat tggtccaacc agaatctgct tggatttgcc catcaaactt ggaaccaata
     2341 gtttccaagg tgcggataga agtggttccg acagcgatga cgcgaccacc attttctttg
     2401 acagagcgaa gggtggcagc agcttcctca gaaagttgat agaactctga atgcatttcg
     2461 tgttcgtcca gattatccac agaaacaggt ctaaaggttc cgagtccgac atggagagtc
     2521 agatagacta gatgaacacc cttagcttgg atttctgcca gcagttcttt ggtgaagtga
     2581 agaccagcag tcggtgctgc agcagagccg ctttccttgg cgtagacggt ttgataacgt
     2641 tcacggtcat ctaatttttc gtggatataa ggtggcagag gcatttctcc cagactttcc
     2701 aagacttcta ggaaaattcc ttggtattca aagcggacaa tgcgtccccc gtgggtcaat
     2761 tcttctgtaa cgacagcgct gaggcggcca tcaccaaagc tgatacgagt accgaccttg
     2821 aggcgtttgg caggtttagc cagaacttcc cactcgtctc cactagtgtt cttaaggagg
     2881 agaagttcca catgacctcc tgtttccact ttttgaccat agaggcgggc agggagaact
     2941 cgggtgtcgt tcatgacaag ggcatcacca ggttccagca tatcaataat agagtggaag
     3001 tgtttatctt gcatttctcc tgtctcacgg ttgacgatga ggagtttgga ggcatcacgt
     3061 ttttcaaggg gcgtttgggc aatcaattcc tctggcaagt ggaaatcaaa atcagctgta
     3121 ttcatatatc tgttcattct ttctaagttc taatcctatc cattataaca cgtttcaagt
     3181 ttggacgctc tttttttaga aattaaatcg ttttcacttg acaaaaattg gtctatacca
     3241 tataataaat atagacagaa atggaggatg aaaagatgaa agttattaaa gttgaaaacc
     3301 aagttcaagg tggaaaagtt gcttttgaga ttttgaagga aaaattggct aatggagctc
     3361 aaactctagg acttgcgaca ggaagtagcc cacttgaatt ttacaaggaa attgttgaga
     3421 gtgaccttga tttttcaaat ctaaccagtg tcaaccttga tgagtatgtg ggccttgatg
     3481 gggacaatcc acagtcttat cgttacttca tgcaagaaaa cttgttcaac caaaaaccat
     3541 ttaaagaaag tttcttgcct cgtggtgtta aggacaatgc tgaagctgaa gttgaacgct
     3601 acaaccaaat tttggctgac catccagttg acctccaaat cttgggaatc ggtcgcaatg
     3661 ggcatatcgg ctttaatgag cctggtactc catttgacag tcaaacgcat ctagtagaac
     3721 ttgaccagtc tactatcgaa gccaatgcac gcttctttgc taagattgaa gacgtcccaa
     3781 cccaagctat ttcaatgggg attaaaaaca ttttggatgc caagtctatt attctttttg
     3841 cttacggtga gtcgaaagca gaagccattg ccggaacagt atctggccca gtgactgaga
     3901 acctacctgc aagtagcctc caaaatcacc ctgatgtaac catcatcgca gatgctgaag
     3961 cgcttagctt actcgaaaaa taaaaaagca atgttctatt gaacgctttc aactcttgta
     4021 taaatggaag aaaaaatcaa aattaaaccg catttttgct tgacaattat tccttttacg
     4081 tgtaaaatag aatagatctt gaacttgaag ggagtgaaaa aaatgtctaa aacagtagta
     4141 cgtaagaatg aatctcttga cgacgcactt cgccgtttca aacgtgcggt tactaaagct
     4201 ggtactcttc aagaaacacg taaacgtgaa ttctatgaaa aaccttctgt aaaacgtaaa
     4261 cgtaaatcag aagtagctcg taaacgtaaa aaattctaat tgaaatgaaa ggctagagaa
     4321 atctagtcct ttttctttta aataaatact ccaaagcctg caaaaatctg aaacttcctc
     4381 ctacaatttg atataataga gagaagaact catttgaagg aggaaatgat gtcggtttta
     4441 gtaaaagaag tgattgaaaa gcttagacta gacattgtct atggtgaacc agaattgctt
     4501 gaaaaggaaa tcaatacagc ggatattacg cgacctggtc ttgaaatgac aggctatttt
     4561 gactactata caccagagcg gattcaactt ttggggatga aggagtggtc ttatctgatc
     4621 agcatgcctt ccaacagccg ttatgaagtt ttgaaaaaaa tgtttctacc tgagacacca
     4681 gcagtcattg ttgcccgtgg tttggtggtt ccagaggaga tgttaaaggc tgctagagaa
     4741 tgtaagattg ctattttaac cagccgtgca gctaccagtc gtttatctgg agagttatct
     4801 agctatctgg attctcgttt ggcagaacgt accagtgtac acggtgtctt gatggatatt
     4861 tatgggatgg gcgtcttgat tcagggagat agtggaattg gtaagagcga gacaggtctt
     4921 gagcttgtca aacgtggtca ccgtttggta gccgatgacc gtgtcgatat ctttgccaag
     4981 gatgagatta ctctctgggg tgaaccagct gaaattttga aacacttgat tgaaattcgt
     5041 ggggttggta ttatcgatgt tatgagtctc tacggtgcga gtgctgtcaa ggattcttca
     5101 caggttcagc ttgctgtcta tttggaaaat tacgatacgc ataagacctt tgatcgtctt
     5161 ggaaacaatg cagaggaact tgaagtttct ggcgtagcca ttcctcgtat tcgtattcca
     5221 gttaaaacag gtcgtaatat ctctgttgtg attgaggcag ctgccatgaa ttatcgtgcc
     5281 aaggaaatgg gctttgatgc gacccgtttg ttcgacgaac gactgacaag tctcatcgct
     5341 cgaaatgagg tgcaaaatgc ttgatccaat tgctattcaa ctaggacccc tagccattcg
     5401 ttggtatgcc ttatgtattg tgacaggctt gattcttgcg gtttatttga ccatgaaaga
     5461 agcacctaga aagaagatca taccagacga tattttagat tttatcttag tagcctttcc
     5521 cttggctatt ttaggagccc gtctctacta tgttattttc cgatttgatt actatagtca
     5581 gaatttagga gagatttttg ccatttggaa tggtggtttg gccatttacg gtggtttgat
     5641 aactggggct cttgtgctct atatctttgc tgaccgtaaa ctcatcaata cttgggattt
     5701 tttagatatt gcggcgccta gcgttatgat tgctcaaagt ttggggcgtt ggggtaattt
     5761 ctttaaccaa gaagcttatg gtgcaacagt ggataatctg gattatctac ctggctttat
     5821 ccgtgaccag atgtatattg aggggagcta ccgtcaaccg actttccttt atgagtctct
     5881 atggaatctg cttggctttg ccttgattct gatttttaga cggaaatgga agagtctcag
     5941 acgaggtcat atcacggcct tttacttgat ttggtatggt ttcggtcgta tggttatcga
     6001 aggtatgcga acagatagtc tcatgttctt cggctttcga gtgtcccaat ggctgtcagt
     6061 tgtccttatc ggtctcggta taatgatcgt tatttatcaa aatcgaaaga aggcccctta
     6121 ctatattaca gaggaggaaa actaaatgtt agaagttgca tatattcttg ttgccctagc
     6181 tttgattgtc tttttggtct atctgatcat tactgtacaa aagcttggtc gtgtcatcga
     6241 tgaaacagaa aagacgatta aaaccttgac ttcagatgtg gatgtgacct tgcatcacac
     6301 caatgagttg ttggctaagg tcaatgtctt ggcagatgat atcaatgtca aggtggctac
     6361 gattgatcca ctcttcagtg ctgttgcaga tttatctcta tctgtttcag acctcaatga
     6421 ccatgcgcgt gtcttgagca agaaagcttc atcagctggt tcaaaaacac tcaagactgg
     6481 tgcaagtctg tcagctcttc gtcttgcaag taaatttttc aaaaaataaa aaaggagaat
     6541 ccttatgggt aaattatcct caatcctttt aggaacggtt tcaggtgcag ctcttgcctt
     6601 gtttttaaca agtgataagg gcaaacaaat ttgcagtcag gctcaagatt ttctagatga
     6661 tttgagagaa gatccggagt atgccaagga gcaagtctgt gaaaaactga cagaagttaa
     6721 ggagcaggct acagattttg ttctgaaaac aaaagaacag gttgagtcag gtgaaatcac
     6781 tgtggacagt atacttgctc aagctaaatc ctatgctttt caagcgacag aagcatcaaa
     6841 aaatcaatta aataatctca aggaacaatg gcaagaaaaa gccgaagctc ttgatgactc
     6901 agaagagatt gtgattgata taacagaaga ataaaccatc accatctccg aacggactat
     6961 gtatctgggg atggtgattt ttatctggaa tctagtcttt gtggtataat aattactatg
     7021 cagaaaaaac caacgtcagc ctatgtgcac atcccatttt gtacccagat ttgttattat
     7081 tgtgattttt caaaggtctt catcaaaaat cagccagtcg acagctattt agagcatctg
     7141 ctggaagagt ttcgttctta tgatattgaa aagttgtcaa ccctttatat cggtggtgga
     7201 acaccgacag ccctgtcggc tccgcaactg gaggttttac tgaatggctt gactaaaaac
     7261 ttggacttgt ctgtcttaga agagttgacc attgaagcca atccaggcga tttggatgcg
     7321 gataagatag ctgttttgaa aaattcggct gtcaatcgtg tttcgctagg tgtccagacc
     7381 tttgatgata agatgttgaa aaagattggg cgcagtcatt tggagaagga tatttatgaa
     7441 aatatcgatc gcctgaaact ggctggtttt gacaacatct ccattgattt gatttatgct
     7501 ctgcctggtc agaccatgga gcaagtaaag gaaaatgtgg ctaaagccat tggactggat
     7561 attccccaca tgagtttgta tagtttgatt ttagaaaacc atacggtctt tatgaaccgg
     7621 atgcgacgtg ggaaattgcc tctgcctaag gaggaactag aagcggagat gtttgagtac
     7681 atcattgcag agctggagcg agcgggtttt gagcattatg agatttctaa tttctccaaa
     7741 cccggctttg aaagtcgtca caatctcatg tactgggaca atgctgaata ctatggtatt
     7801 ggtgcagggg catctggtta tgtcaatgga gtacgctata aaaatcatgg tcccattcgt
     7861 cattatctca gtgcggttga ggaaggcaat gcttgtatta cagaagatca cctgagtcaa
     7921 aaggagcaaa tggaagaaga aatgttcttg ggactccgca agaaatccgg ggtttccatg
     7981 gcgcgatttg aggaaaaatt tggacagtct tttgctggac tttatggaga aattgtcaga
     8041 gatttggttc aacaaggcct catgcagatt gagggtgacc acgttcgcat gacaaagaga
     8101 ggtctctttt tgggagatac tgtagcagaa cgatttattt tggagtagga tcatgggctt
     8161 aacttaccaa atgaaaatga aaattccttt tgatatggct gatatgaacg gtcatatcaa
     8221 acttccagat gtgattttgc tgtcactgca agtttcaggg atgcaatcga ttgaactggg
     8281 agttagtgat aaggccattt tggaagacta taatctggtc tggattatcg cagaatatga
     8341 tattgaggtg gttcgtttgc ctcgttttgc ggaagaaatt accatcgaaa cggaagcctt
     8401 gagctacaat cgactttttt gctaccgtcg ctttactatt tataatgaag cggggcagga
     8461 gctcatccat atgatggcga cctttgttct catggaccgc gacagtcgaa aagtccatgc
     8521 tgttgaacct gagattgtgg ctccttacca gtctgatttt gataaaaaac ttatccgtgg
     8581 accgaagtat gagtccttgg aagaaccgat cagcaaggat taccatgttc gtttttacga
     8641 cttggatatg aatggtcatg tcaataacag taaatacttg gactggattt ttgaggtcat
     8701 gggagcggat tttttgaccc actatattcc caagaaaatc aacctcaagt atgtcaagga
     8761 agttcgacca ggtggagtga ttacatcggt tgttgaacgg actggactgg aaagcaagca
     8821 tgaaattaca agtgatgggg ttatcaatgc ccaagcaatc attacttggc aagaaataaa
     8881 aaaggcttag ggagaaatag atgaaatata aaggctattt aattgattta gacggaacca
     8941 tttataaggg gaaagaccga atccctgctg gagagacttt tgttcatgaa ttgcaaaagc
     9001 ggggcattcc ctatctcttt gtaaccaaca atacaactcg cactccagag agtgttaagg
     9061 agatgttggc tcagaatttt aatatcgata cgcccctatc gactgtctac acagcgactt
     9121 tggcaaccat cgactatatg aatgatttgg ggcttgaaaa gaccgtctat gtcgtcggag
     9181 aagcaggact caaggaagcc atcaaggcgg ctggttatgt ggaagacaag gaaaaccctg
     9241 cctacgtggt agttggtctg gattggcaag tcgactatga aaaatttgcc acagcaactc
     9301 ttgctattca aaagggtgcc cattttatcg gaaccaatcc tgacctcaac atcccgacgg
     9361 aacgcggtct tttgccaggt gctggctcac tgattacgct gcttgaagta gcgacacgag
     9421 tgaagccggt ttatatcgga aaacccaatg ccatcattat ggacaaggcg gttgagcact
     9481 taggtttgga acgtgaagag ttaatcatgg ttggggacaa ttatttgact gatatccggg
     9541 ctgggattga caatggcatt ccaacgctct tggtgacgac aggttttact aaggcagaag
     9601 aagtagctgg cctgccaatc gcaccgacac atgtggtttc tagccttgcg gagtgggatt
     9661 ttgatgaaaa ctaaattgac cttttggggc tctatgctct ttctcctctc cctctcaatc
     9721 cttctgacca tttatctggc ttggattttc tatcctatgg agattcagtg gctaaactta
     9781 acaaatcgag tctatctaaa accagaaacc attcaataca attttcatat cttaatgaat
     9841 tatctgacca atccttttag tcaggtctta cagatgcctg attttcgttc gtcagcagct
     9901 ggtctgcacc atttcgcagt ggtcaagaat ctctttcatt tggctcagct agtagctcta
     9961 gtgacactgc caagtttcta tgtctttgtc aataggattg tgaaaaagga ctttttgtct
    10021 ctttatcgaa aaagtctcct gactctagta gtcttacctg tgatgattgg acttggggga
    10081 gttttgattg gttttgacca attctttact cttttccatc aaattctctt tgtgggagat
    10141 gatacctggc tttttgatcc agccaaggat cctgttatta tgattttgcc agagaccttc
    10201 tttcttcatg ccttcctcct cttttttgcc ctctatgaaa gcttctttgg ctatctgtgt
    10261 ctgaaaagtc gtaggaagtg aaatgaaaga tgtttttaat t
//
LOCUS       DAVGJL010000073        10289 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.073, whole genome shotgun sequence.
ACCESSION   DAVGJL010000073 DAVGJL010000000
VERSION     DAVGJL010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10289
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.073"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..144
                     /locus_tag="VVE42_001410"
     CDS             <1..144
                     /locus_tag="VVE42_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEU3073593.1"
                     /translation="KERSKVENIFAKVKTFKMFSTTYRNHRKRFGLRMNLIAGIINHE
                     LGF"
     gene            212..460
                     /locus_tag="VVE42_001411"
     CDS             212..460
                     /locus_tag="VVE42_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073594.1"
                     /translation="MVSSSGSEFQSGWQEHQLIAEKVSKTLDKTFDKDVREIPTSQFY
                     QKFVDEMGRIYSGNLILQELITVNGAYKATYIGELSSN"
     gene            591..1763
                     /locus_tag="VVE42_001412"
     CDS             591..1763
                     /locus_tag="VVE42_001412"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEU3073595.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENTDSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLDKDRLN
                     KLNQRTALSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            1763..3037
                     /locus_tag="VVE42_001413"
     CDS             1763..3037
                     /locus_tag="VVE42_001413"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEU3073596.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNGQEYTVPYVTEEPSVVAAASYASKIIKRAGGFT
                     AQVHQRQMIGQVALYQVANPKLAQEKIASKKAELLELANQAYPSIVKRGGGARDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLMGILSNYATDSL
                     VTASCRMAFRYLSRQKDQGREIAEKIALASQFAQADPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VALSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVERLISDKTFNLETAQRYLENLRS"
     gene            complement(3073..4038)
                     /locus_tag="VVE42_001414"
     CDS             complement(3073..4038)
                     /locus_tag="VVE42_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU3073597.1"
                     /translation="MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     GYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQSIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTREKPDAIFASDDLTAILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKSI"
     gene            complement(4019..5473)
                     /locus_tag="VVE42_001415"
     CDS             complement(4019..5473)
                     /locus_tag="VVE42_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEU3073598.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWAQLESDDLVHFKETGIKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDENWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFKGQYYAVVGGQDLEKKGFVRLYKAVNNDYTNWQAVGDLDFANDRT
                     AYMMECPNLVFVEEQPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRALAVSWLGLPDVSYPSDRFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRASEEAFSNRSQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNGQVTVDRSQAGEQYAQEFGTTRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGTYYELDYGRKTN"
     gene            5850..7733
                     /locus_tag="VVE42_001416"
     CDS             5850..7733
                     /locus_tag="VVE42_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEU3073599.1"
                     /translation="MNNQEIAKKVIDALGGRENVNSVAHCATRLRVMVKDEEKINKEV
                     IENLEKVQGAFFNSGQYQIIFGTGTVNKMYDEVVVLGLPTSSKDDMKAEVAKQGNWFQ
                     RAIRTFGDVFVPIIPVIVATGLFMGVRGLFNALEMPLPGDFATYTQILTDTAFIILPG
                     LVVWSTFRVFGGNPAVGIVLGMMLVSGSLPNAWAVAQGGEVTAMNFFGFIPVVGLQGS
                     VLPAFIIGVVGAKFEKAVRKVVPDVIDLLVTPFVTLLVMSILGLFVIGPVFHVVENYI
                     LIATKAILSMPFGLGGFLIGGVHQLIVVSGVHHIFNLLEVQLLAADHANPFNAIITAA
                     MTAQGAATVAVGVKTKNPKLKTLAFPAALSAFLGITEPAIFGVNLRFRKPFFLSLIAG
                     AIGGGLASILGLAGTGNGITIIPGTMLYVGNGQLPQYLLMVAVSFALGFALTYMFGYE
                     DEVDATAAAKQAEVAEEKEEVAPAALQNETLVTPIVGDVVALADVNDPVFSSGAMGQG
                     IAVKPSQGVVYAPADAEVSIAFPTGHAFGLKTRNGAEVLIHVGIDTVSMNGDGFEAKV
                     AQGNKVKAGDVLGTFDSNKIAAAGLDDTTMVIVTNTADYASVAPVATGSVAKGNAVIE
                     VKI"
     gene            7882..8769
                     /locus_tag="VVE42_001417"
     CDS             7882..8769
                     /locus_tag="VVE42_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU3073600.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEMVARNNAGGRIENLVYYTIGTGIGAGVIQRGEFIGGVGHPEMGHY
                     YVARHPMDIEKEFKGVCPFHKGCLEGYAAGPSLEARTGVRGETIELNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEVSK"
     gene            complement(9089..9706)
                     /locus_tag="VVE42_001418"
     CDS             complement(9089..9706)
                     /locus_tag="VVE42_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEU3073601.1"
                     /translation="MSQDKQMKAVSPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQAGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
     gene            complement(10121..>10289)
                     /locus_tag="VVE42_001419"
     CDS             complement(10121..>10289)
                     /locus_tag="VVE42_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEU3073602.1"
                     /translation="RLIHRWLPQGTKKTTTKEVAFIEKWINNYPKKCLNYKSPREDFW
                     MTNLNLKFSNN"
BASE COUNT         2976 a         2040 c         2298 g         2975 t
ORIGIN      
        1 aaggagagaa gcaaggttga gaacatcttt gccaaagtaa aaacgtttaa aatgttttca
       61 acaacctatc gaaatcatcg taaacgcttc ggattacgaa tgaatttgat tgctggtatt
      121 atcaatcatg aactaggatt ctagttttgc aggaagtcta ctactttcct tattgtctgt
      181 aaatctactg accttgttgt ttatcccagt catggtttct agttcgggct cagagtttca
      241 aagtggatgg caagagcatc aattgattgc tgagaaggtt agtaaaacac ttgacaagac
      301 atttgataag gatgtcagag aaattccgac cagtcagttt tatcaaaaat ttgtagatga
      361 gatgggaagg atttactcag gaaatttgat cctccaggag ctgataactg tgaatggagc
      421 ttataaagct acttatatcg gtgaactctc tagcaactaa tcattcttta aaatgatgac
      481 caataagagg cttgatttgg tgaatcaagt cttttttctc tcttttactg ccatttgtga
      541 taaaatagta tagaacgatt ttttacatga atgataaaac agaggtaaat atgacaatcg
      601 gtattgataa gattggtttt gcgaccagtc aatatgtctt gaaattacaa gacttagcag
      661 aagcgagggg aattgaccct gaaaaattaa gtaaaggact cttactcaag gaattgagta
      721 ttgcgcccct aactgaggac atcgtgacct tggcggccag tgctagtgac tctattttaa
      781 ctgagcaaga aagacaagaa gttgacatgg tcattgtggc taccgagtca ggaattgacc
      841 agagtaaggc tgcggccgtc tttgtgcatg gcttgctggg catccagccc tttgctcgta
      901 gtttcgagat taaagaagcc tgctacggag cgactgctgc cctccattat gccaaattgc
      961 atgtggaaaa ttctccggag tccaaggtct tggtcattgc cagtgatatt gccaaatacg
     1021 gtattgaaac tccaggagaa ccaactcaag gtgctggaag tgtagctatg ttgattacac
     1081 aaaatccacg catgatggcc tttaataatg acaatgtagc tcagacccgt gacatcatgg
     1141 atttctggcg accaaattac tcgacaactc cttatgtaaa tggtgtctat tctacccaac
     1201 aatacttgga tagtttgaaa acgacttggc ttgaatatca aaaacgctac cagcttactt
     1261 tggatgattt tgcggctgtt tgtttccact tgccttatcc taaattagcg ctaaaaggct
     1321 tgaaaaaaat catggataag agcctgcctc aagagaaaaa agacctctta caaaagcatt
     1381 ttgaccagtc tattctctac agtcaaaagg tggggaatat ctacacaggt tcacttttcc
     1441 ttggactttt gtctctcttg gaaaatacag atagcttgaa agctggggat aaaatcgccc
     1501 tttatagtta cggaagtgga gctgtggctg agttcttcag tggtgaattg gttgaaggat
     1561 atgaagctta tttggataaa gaccgtttga acaagctcaa ccaacgaact gccctatccg
     1621 ttgcagacta tgaaaaggtc ttttttgagg aagtaaactt ggatgaaaca aactctgccc
     1681 agtttgctgg ctatgaaaat caagattttg ccttggttga aattctcgac caccaacgcc
     1741 gttatagcaa ggttgaaaaa taatgaagat aagttggaat ggattttcta aaaaatcata
     1801 ccaagagcgc ctcgagctgt taaaagctca ggcgctcctt agtcctgaga gacaagctag
     1861 tctggagaag gatgaacaga tgagcgtgac tgtggcagac cagctgagtg agaatgtagt
     1921 gggaactttt tctctgcctt attcactggt tccagaggta cttgtcaacg gtcaggaata
     1981 caccgttccc tatgtgacag aagaaccctc tgtggttgcg gcggccagct atgccagcaa
     2041 aatcatcaag cgtgcaggtg gttttactgc acaagtccat cagcgacaga tgattgggca
     2101 ggtagccctt tatcaagttg ctaatcctaa actagcgcaa gagaagattg ccagcaagaa
     2161 agcggagctc ttggagcttg ccaatcaagc ctatccttct atcgttaaac gtggaggtgg
     2221 ggcgcgtgat ctgcatgtcg agcagataaa aggcgaacca gactttctcg ttgtttatat
     2281 tcatgtcgat acccaggaag ccatgggtgc caatatgctc aacaccatgc tggaagcctt
     2341 gaaaccagtc ttagaagaac tcagtcaggg acagagtctc atgggaatcc tgtccaacta
     2401 cgcgaccgat tctctggtga ctgcaagctg tcgcatggcc tttcgctact tgagccgcca
     2461 aaaggatcaa ggacgagaga ttgcggagaa aattgcattg gctagtcagt ttgcgcaggc
     2521 tgatccttac cgagctgcta ctcataataa aggaattttt aatggtattg atgcgatttt
     2581 gattgccact ggtaatgact ggcgtgccat cgaagctggg gcccatgcct ttgccagtcg
     2641 agatggacgc tatcaaggtc ttagctgctg gacgctggac cttgaaagag aagaattggt
     2701 cggtgagatg accctgccca tgcctgtagc gactaagggt ggctctatcg gcctcaaccc
     2761 acgtgtagct ctcagtcatg atctactagg aaatccttct gccagagaat tagcccagat
     2821 tatcgtgtcc atcggtcttg ctcaaaattt tgcagccctc aaagctttgg taagtacggg
     2881 catccagcaa ggccacatga aactacaggc caaatcccta gctctcctag ctggggctag
     2941 tgaatctgaa gttgctcccc tagtagagcg cctcatctca gataaaacct ttaacctaga
     3001 gacagcccag cgctatctcg aaaatttaag atcataaaaa actcagacga atcggtctga
     3061 gttttcttgt gtttaaatac tttttcctgg taatagtgta actggtaaga agtaaccagt
     3121 tgtggcaact tccttgcctt caatcttttg caagagaaga tcaatagtga gacaagcaat
     3181 ctcttccaaa ggttgcttga tagtagccaa ttgagggtag taattttcga taaagtaggt
     3241 cccatcatag ccgatgacct tgagctcttt tgggacagaa atgcccaatt cttgagcgat
     3301 tttaatgacc agaatagctg tcaaatcatc cgaagcaaaa atggcatctg gtttttcccg
     3361 ggtcaagata ttcttgattt ccatttcttt tctgacggga gaaaagtcac tggaaacatt
     3421 gataatagga gcttttggga gtacggatgc aaaaccagcg tggcgtagtc cggttggcga
     3481 attggaattg tcattccctg taatcatgat gatagactgg gcacctgtct tgaccaaggt
     3541 ttgggcagca agaaccccac cagcatagtt gtcagaggag acgacaggga tgtctggcga
     3601 taggtttcgg tcaaaggaaa taatcggcgc tgtcacacga ttgtagtctt cgattcctag
     3661 gttgtgacta ccagaaatga tgccgtccac ctgattggct tccaacattt cgatgtattc
     3721 gcgttccttc tcagaatcat gttcactgtt gcagatgatg gtcttgtaac catttttgaa
     3781 gagttggtgt tccaatttat caatcaattc tgcatagaaa acattggaaa tattggggaa
     3841 aatcaagccg attaacttag ctgattttcc ttgcagacta cgagccaggt tgttgggttt
     3901 atagcccaat tctcgcatgg cttcattgac tttttggatg gttttctcag atagataccc
     3961 ttttttattg ataacccgag aaacggtagt aggactgacg cctgcaagtt tggcgacatc
     4021 agttagtttt gcgaccataa tctaattcat agtaagttcc agttgggttt ccagatttga
     4081 tcaggatacc attttggtcc gcatgtggga agacacgacc agaaaatact ttttctcctt
     4141 tattgatgaa aatttcaaag acagagttat cgatgaagat tgtagcagta gtagcctgat
     4201 tctcgatagg gcaagagcga gttgtcccaa attcttgggc atactgttct ccagcctggc
     4261 tacgatccac tgtcacttga ccgtttacaa ggtcaaagtt gattgaaagt cccttacctt
     4321 ctttatcagc aagtaagaca atctcgctct ggctattagc ttccaagttg agttcaagtt
     4381 cgtaagtgtt cttggtttgg gaacggtttg agaaggcttc ttcagaagca cgaaggtcct
     4441 taatagcagc gactggatac tggtagagct tgtcgtcttt gatagtgagt tccttgacca
     4501 aagagaaggt tccttggtgg tcaaaacggt cagatgggta agaaacatct ggcaaaccaa
     4561 gccagctaac tgctagagca cgcccatcag gagcgttgaa ggcttgagtt gcataggctt
     4621 cgaaaccgta atccatgttt tgaagttgag acacatctac cattttggca tttttagggt
     4681 caaaggaagc cccaatctta tacatatttg gaaagatatt atcgtagtct agaactttct
     4741 tatccaatcc ttgtggacag tagagaagga caggctgttc ctctacaaag accagattag
     4801 gacattccat catgtaggca gtacggtcgt tagcaaagtc aaggtcgcca actgcttgcc
     4861 agtttgtgta gtcgttattg acagccttgt agagacggac gaagcctttt ttctccaagt
     4921 cctgtccacc gacgacagca tagtattgac ctttaaagtt aaaaatttgt ggatcgcgga
     4981 agtggtcagt agagtctgct ggctggtcaa tcaagatctt gtcaatcttt gtaatcttac
     5041 cctccttgtc catcaaagca ccgatttggt atgggtgacg gatccagttt tcatcgcgaa
     5101 catttcctgt ataaaatagg aataagttat cgccaaattg catggcagaa ccagagtagg
     5161 caccgtggct atctaatgga gtatccggca aaactttgat tccagtttct ttaaagtgaa
     5221 ccaaatcatc actttctagc tgtgcccaag atttcaaacc gtgggctgca ccaaaaggaa
     5281 aattctggta aaagaggatc cacttgccat caaagtaaga aaagccattt gggtcgttga
     5341 gaagtcctgt ttttggctca acatggtaat gagtatgcca tggagattgt gccatatttt
     5401 cctttatatg ttgaatttct tcttgcgtcc aatcttgata aagtctgtaa cgacgctcag
     5461 ttgtccattc cattcattcc tccaaaaatc ttatcacttt taatagtaac tgttttcgat
     5521 aaaaaatgca agccttttat gttaaaaaag aggaaaaact atcaaacgat tatcataaaa
     5581 taaatacagg aaatcaatca aatttttacg aaaatgtgaa agataatgaa ataaaagact
     5641 ttttaatcaa ggtttcaaac ttatttttca aggaatccct gatgaaacag tcaaaaacaa
     5701 tcaatctaaa gcttgcccgc taagtggata gaatattttt tctgcaaaac gcttgacata
     5761 tttttaaaac atgataaaat aatagtcgta agggcgaata aaatcgcttt caaacaagaa
     5821 caaaatgtta tataaggaga tttttgcaaa tgaacaatca ggaaattgca aaaaaagtca
     5881 tcgatgcctt gggcggacgt gaaaatgtca atagtgttgc ccactgtgcg actcgtctac
     5941 gtgtcatggt caaagatgaa gagaaaatca ataaagaagt gattgagaac ttggaaaaag
     6001 ttcaaggtgc tttctttaac tcagggcaat accaaattat ctttggtaca ggtacagtta
     6061 acaaaatgta cgatgaagtt gttgtacttg gcttaccaac atcatctaag gatgacatga
     6121 aagcagaagt tgctaaacaa gggaactggt tccaacgtgc tatccgtact ttcggtgatg
     6181 ttttcgttcc aatcatccca gttatcgtag cgacaggtct cttcatgggt gtgcgtggtc
     6241 ttttcaacgc tcttgaaatg ccacttccag gtgactttgc aacttacaca caaatcttga
     6301 cagatacagc cttcatcatc ttgccaggtt tggttgtgtg gtcaaccttc cgtgtatttg
     6361 gtggaaatcc tgccgttggt atcgttcttg gtatgatgct tgtctctggc tcacttccaa
     6421 acgcttgggc agttgctcaa ggtggtgaag taacagcgat gaacttcttt ggtttcatcc
     6481 ctgttgttgg tttgcaaggt tccgttcttc cagccttcat catcggggtt gtcggagcta
     6541 aatttgaaaa agctgtccgc aaggttgttc cagatgtcat tgacctcttg gtaacaccat
     6601 tcgtgacact tttggtcatg tctatccttg gactctttgt cattggacca gttttccacg
     6661 ttgttgaaaa ctacatcctt attgctacaa aagcgattct tagcatgcca tttggtcttg
     6721 gtggtttctt gattggtggg gttcaccaat tgatcgtcgt gtcaggtgtg caccacatct
     6781 tcaacttgct tgaagtgcaa ttacttgctg ctgaccatgc taacccattc aacgctatca
     6841 tcacagctgc tatgacagct caaggtgctg ctactgttgc ggttggtgtt aaaacaaaaa
     6901 atccaaaact gaaaacactt gctttcccgg ctgctctttc tgccttccta ggtattacag
     6961 agcctgctat cttcggggtg aacttgcgct tccgtaaacc attcttcctt tcattgattg
     7021 ctggtgcaat cggtggtgga ttggcttcta tccttggact tgctggtact ggtaatggta
     7081 tcaccatcat ccctggtaca atgctttatg ttggtaacgg acaacttcca caataccttc
     7141 ttatggtagc tgtatcattt gcccttggtt ttgctcttac ttacatgttt ggttacgaag
     7201 atgaagtaga cgcaactgca gctgcaaaac aagctgaagt ggctgaagaa aaagaagaag
     7261 ttgcgccagc agctcttcaa aatgaaacac ttgtaactcc tatcgttggt gatgttgtcg
     7321 ctcttgctga tgtcaatgac ccagtcttct caagtggagc tatgggacaa ggtatcgctg
     7381 tgaaaccaag ccaaggcgtg gtctatgcac cagctgatgc tgaagtttca attgcctttc
     7441 caacagggca cgcttttggt ttgaaaacaa gaaatggtgc tgaagttttg attcacgttg
     7501 gtattgacac tgtatctatg aacggtgacg gttttgaagc aaaagttgct caaggtaata
     7561 aggtgaaagc tggcgatgtt cttggaacat ttgactcaaa caaaatcgct gcagctggac
     7621 ttgatgatac aacaatggtt atcgttacaa atacagctga ctacgcttca gtagctccag
     7681 tcgcaacagg ttcagttgcg aaggggaatg ctgtgatcga agtgaaaatc taatcaatcc
     7741 tctctaatgt gaaaacgaac aaatgacatg tttgttcgtt tttattagat agtaagattt
     7801 acagaggaaa atctaaaaaa tagagacatt tagactttcg aagtatgcta taataaagaa
     7861 aataaaaaca agaggtttat catgacaaaa ttatatggaa gcttggaagc gggcggtaca
     7921 aagtttgtct gtgctgtcgg tgatgaaaac tttaacgttg tagaaaaaac acaatttcca
     7981 acaacaactc caatcgaaac aatcgataaa accattgagt tcttctcaaa attcgataac
     8041 cttgctggtc ttgcagttgg ttcatttggg ccgattgata ttgacaaaaa ctcaaaaact
     8101 tatggcttta tcacgacgac tccaaaacca aactgggcaa atgtggactt gcttggtgcc
     8161 cttcgtcgcg ccctaaacgt gccaatgtac ttcacaacag acgtaaacag ctctgcttac
     8221 ggtgaaatgg ttgcccgtaa caatgctggt ggtcgtatcg aaaacttggt ttactacaca
     8281 atcggtacag gtatcggtgc aggcgtcatc caacgtggtg agtttatcgg tggtgtgggt
     8341 caccctgaaa tgggtcatta ttatgttgct agacacccaa tggatattga aaaagagttt
     8401 aagggtgttt gtcctttcca taagggatgt ctagaaggtt atgcagctgg tccaagtttg
     8461 gaagctcgta caggtgtacg tggggaaact attgaactca acaaccctgt ttgggatgtt
     8521 caagcctact atatcgctca agctgcggtt aatgcgacag tgactttccg cccagacgtg
     8581 attgtctttg gtggaggggt catggctcaa caacatatgc tggaccgtgt ccgtgagaaa
     8641 tttacatctc ttcttaatgg ttacctacca gtaccagatg tgcgtgacta tatcgtgacg
     8701 ccagcagtcg caggaaatgg ttctgccaca cttgggaact ttgttcttgc aaaagaagtt
     8761 tcaaaataaa gcacaaaatc cgcttgggaa accaaacgga ttttgtggtt gaaaacaata
     8821 tttttgagcc tttccttaag tgattacgga cggtagcgac tttcttcgaa attccatacc
     8881 taaactttga gcctagtgtc tcaaagtttc cgaatacctg aaaccattag atttcaggtg
     8941 tttctatcac ggcggaaagg ctaggtaagt cctcgcctca ctatcgaaaa atatgcatga
     9001 tttccatacc aaagtatatt tagtatcaag ctatagttaa tctacgcttt aaaaatccac
     9061 ttgggaaacc aaacggattt tttacgtgtc aaagcatttg ccagaaaaag tcaataatat
     9121 aggtcagacc gtaggtataa accacgaggg taaagggttt ggtcagaatg atgatggtca
     9181 tgtagcgctt gaaactcatc ttggtcaggg cagccagcat acagagaaag tcagctgggc
     9241 taatgggcca aatcatcata aaaataaaga agcggtcaaa acgattgccc ttatctagcc
     9301 agccgatgta cttgtcgtag gtgcgcttgc tgacgacaga ctggacaaag gcagctccgt
     9361 ataggcgcac tagataaaag ataatggcac agccaatcac gatgccgata tagttgtaga
     9421 tagtcccgat gatgtgcccg tagataaaga ccccagccac cgaggtcaag gcccctggaa
     9481 tgatagggac gacagtctgt aaaatctgta aaaagataaa gagaggtgga ccccagatgc
     9541 ctgcctgctg gataaaggca gagagggttt ccttggattg taaaatccca gcctgataag
     9601 cccaaataca gaaaatcaaa ctcccaaccc caccgacaat cgatgagata ttgataactc
     9661 gctgcagaag gggagaaaca gctttcattt gtttatcctg tgacatgtaa actcctcata
     9721 gatagtatat ttctactata ccatattttg gaggagaaag ggggaatgag tcttttttga
     9781 taaggaatag aaagtaaata gcccttcgag acttagaata ggtctgtcaa ctaaaggtta
     9841 tatgcattct aggtttcgaa taatcgcaat tccataaatt taaaagcatt ctaagtttcg
     9901 aatagcttat ctgagataat tttgaggcat tcgcgctttc gaatagtcat ttttcctaaa
     9961 gtttagatgt attctgaagc tagaaagact aaaatctaaa agtagtaaga gtctctctct
    10021 gatggacgta atttatggct agtatgaatc ctttgaattg ttagttaatt ttaatattaa
    10081 tcagttctga aataattact aaatatagtt agatggtgaa ttaattattg ctaaatttca
    10141 agttcaagtt agtcatccag aagtcttctc tgggggactt gtagttcaag cattttttag
    10201 gatagttgtt aatccacttt tcgatgaatg cgacttcctt tgtagtcgtt ttcttagttc
    10261 cctgaggtaa ccatctatga atgagtctg
//
LOCUS       DAVGJL010000074        10238 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.074, whole genome shotgun sequence.
ACCESSION   DAVGJL010000074 DAVGJL010000000
VERSION     DAVGJL010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10238
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.074"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            59..385
                     /locus_tag="VVE42_001420"
     CDS             59..385
                     /locus_tag="VVE42_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU3073603.1"
                     /translation="MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEQLLTLKDEWALYTDTINGIIEGS
                     IRHDKN"
     gene            372..965
                     /locus_tag="VVE42_001421"
     CDS             372..965
                     /locus_tag="VVE42_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEU3073604.1"
                     /translation="MTRTEYLTQLELYLKKLPEADRIEAMDYFRELFDDAGVEGEEEL
                     IASLGTPKEAAHEVLSNLLDKKINEAPAQKNNRQILHIALLALLAAPIGIPLGIAILV
                     TLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTILAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYVARFFGLLIVRLVQFVLKKGKRGNQHA"
     gene            958..1869
                     /locus_tag="VVE42_001422"
     CDS             958..1869
                     /locus_tag="VVE42_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HEU3073605.1"
                     /translation="MRKWTKGFLIFGVVTTVIGFILLFVGIQSDGIKSLLSMSKEPVY
                     DSRTEKLTFGKEVENLEITLHQHALTITDSFDDQIHISYHPSLSAHHDLITNQNDRTL
                     SLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILQLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNSYILRIEGSRIKNSKLTTPNIVNIFDTVLTDSQLESTENHFHAEN
                     IQVHGKVELTAKDYLRIILDQKESQRINWDISSNYGSIFQFTREKPESRGTELSNPYK
                     TEKTDVKDQLIARSDDNIDLISTPSRR"
     gene            1932..2996
                     /locus_tag="VVE42_001423"
     CDS             1932..2996
                     /locus_tag="VVE42_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073606.1"
                     /translation="MKQEWFESNDFVKTTSKNKPEEQAQEVADKAEETIADLDTPIGK
                     NTQVEEEVSQAEVELESQQEEKIETPEDSEARTEIEEKKASNSTEEEPDLSKETEKVT
                     IAEESQEALPQQKATTKEPLLISKSLESPYIPDQAPKSRDKWKEQVLDFWSWLVEAIK
                     SPTSKLETSITHSYTAFLLLILFSTPSFFFSIYHIKHAYYGHIASINSRFPEQLAPLT
                     LFSIISILVATTLFFFSFLLGSFVVRRFIHQEKDWTLDKVLQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFTALLCVISIGIILLASLHIITRPSQASETDSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGDYLRVLAFL"
     gene            complement(3068..3928)
                     /locus_tag="VVE42_001424"
     CDS             complement(3068..3928)
                     /locus_tag="VVE42_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 domain-containing protein"
                     /protein_id="HEU3073607.1"
                     /translation="MAKTFFIPNKQSILGEQEILTAKSILALLDGLESHSYDVVYLRQ
                     PLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDLPDLLTKTDWQIIKSFLDALLAYT
                     GTDIEGLDGFDFEAYFQASIQAYLADPVARFTICQGIFNPIFFSRENLKSFLEADGLA
                     QFEARVRAVQETDAYFARVSFYQDGEGKVHGVYHLAQGVKTVLPREPFVPAAYIEQLV
                     DKEVQWEIDLVQITGDGSKPEDYESIARLDYAKFLEVLPPSFYPQLDANQLEIQPILV
                     QDFKTLAQEE"
     gene            complement(4203..5189)
                     /locus_tag="VVE42_001425"
     CDS             complement(4203..5189)
                     /locus_tag="VVE42_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEU3073608.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMEDLWFKIDEENEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            complement(5461..6936)
                     /locus_tag="VVE42_001426"
     CDS             complement(5461..6936)
                     /locus_tag="VVE42_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3502 domain-containing protein"
                     /protein_id="HEU3073609.1"
                     /translation="MKNWKKYAFASASVVALVAGLAACGNLTGNSKKAADSGDKPVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEILNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
     gene            complement(7194..8117)
                     /locus_tag="VVE42_001427"
     CDS             complement(7194..8117)
                     /locus_tag="VVE42_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU3073610.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            complement(8131..9060)
                     /locus_tag="VVE42_001428"
     CDS             complement(8131..9060)
                     /locus_tag="VVE42_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU3073611.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGALWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            complement(9379..9600)
                     /locus_tag="VVE42_001429"
     CDS             complement(9379..9600)
                     /locus_tag="VVE42_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073612.1"
                     /translation="MSLQIKLKKLAKELSKLLKDSNLETVDKDVLENSQKELQKAVLF
                     LADEKGSEHTEAEVIDNLKEVIAKLKANA"
     gene            complement(9554..9814)
                     /locus_tag="VVE42_001430"
     CDS             complement(9554..9814)
                     /locus_tag="VVE42_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073613.1"
                     /translation="MTGVSNPTTDSARLVLAEAKKAFADDSLTEQGLRDILQTVKDAI
                     ASLESIKESQSATKDGGQTAGKETAGEDESANQTQEISQGIK"
BASE COUNT         3089 a         2135 c         1945 g         3069 t
ORIGIN      
        1 atgtatgtgt agaaaaatta tatataaaaa tcttacacat tagaaaagga ggtttcccat
       61 gtactttcca acatcctctg ccttgattga atttctcatc ttggccgtac tggagcaggg
      121 tgattcttat ggttatgaga ttagccaaac cattaagcta atcgctaata tcaaagaatc
      181 cacactctat cccattctca aaaaattgga aggcaatagc tttctgacaa cctattctag
      241 agagttccaa ggtcgcatgc gcaaatacta ctccttgaca aacggtggta tagagcagct
      301 cttgacccta aaagatgaat gggcactcta tacagacacc atcaatggca tcatagaagg
      361 gagtatccgc catgacaaga actgaatacc tgactcagct agaactctat ctcaagaaac
      421 tacctgaagc tgaccgtatc gaagccatgg actatttcag agagctcttt gacgatgctg
      481 gagtcgaagg agaagaagaa ctcatcgcta gtttgggaac tcccaaagaa gcggcccacg
      541 aagttctatc caatcttctc gataaaaaaa tcaatgaagc acccgctcaa aaaaataacc
      601 gacaaatttt acatatcgcc ttgttagccc tccttgcagc acctatcggc attcctctgg
      661 gaatcgccat cctcgtgacc ctgttcgcaa tccttgtagc cgctttgact gtcattctgg
      721 ctttctttgc agtttccata ctgggtatca tcggcggatt cctattttta gttgaaagtt
      781 tcactatcct cgcccaagcc aaatcagcct ttatcttgat ttttggttct ggtttactgg
      841 ctatcggtgc ttcttcgcta gttttacttg gcatttccta tgtagctcgc ttcttcggtc
      901 tactcattgt tcgtctggta caatttgttc ttaaaaaagg aaagagaggt aatcagcatg
      961 cgtaaatgga caaaaggatt tctcatcttt ggtgtggtga ctaccgttat cggctttatc
     1021 ctgctttttg taggtatcca atctgacggg attaagagcc tactttccat gtccaaagaa
     1081 cctgtctatg atagccgtac ggaaaagcta acctttggca aggaagtcga aaacctagaa
     1141 attactctcc accaacacgc gctcaccatc acagactctt tcgatgatca aatccacatt
     1201 tcttaccatc catctctttc tgctcaccat gatcttatca ccaatcagaa cgatagaact
     1261 ctgagtctca ctgataagaa actgtctgaa actccgtttc tctcttctgg aattggtggg
     1321 attcttcata tcgcaagtag ctactctagt cgttttgaag aagttattct ccagctacca
     1381 aaagggagaa ctctaaaagg gatcaacatc tcagccaatc gcggacaaac caccatcata
     1441 aatgctagcc ttgaaaatgc gaccctcaat acaaacagct atatcctccg aattgaagga
     1501 agtcgtatca aaaacagtaa actcacaaca cccaatatcg ttaatatctt tgatacagtt
     1561 cttacagata gtcagctaga gtcaacagag aatcacttcc acgctgaaaa tatccaagtc
     1621 catggtaagg ttgaactgac tgccaaagat tatctcagaa tcatcctaga ccagaaagaa
     1681 agccaacgaa ttaactggga catctcaagc aactatggtt ctatcttcca attcacaaga
     1741 gaaaagcctg aatcaagagg tacggaatta agcaaccctt acaaaactga aaaaaccgat
     1801 gtcaaggatc aactcattgc gagatctgat gataatattg atctaatatc cacaccaagc
     1861 agacgttgac aaaaacgctt acatctgcta ctatggaagc atatttatct aactaaaaag
     1921 gaggttctac catgaaacaa gaatggtttg aaagtaatga ttttgtaaaa acaacaagca
     1981 agaacaagcc tgaagagcaa gctcaagagg ttgcagacaa ggctgaagaa acgatagccg
     2041 atctcgatac accaattgga aaaaatactc aagtagagga ggaagtctct caagctgaag
     2101 tcgaactgga aagccagcaa gaagagaaaa ttgaaactcc tgaagacagt gaagcgagaa
     2161 cagaaataga agaaaagaag gcatctaatt ctactgaaga agagccagac ctttctaaag
     2221 aaacagaaaa agtcactata gctgaagaga gccaagaagc tcttcctcag caaaaagcaa
     2281 ccacgaaaga gccacttctt atcagtaaat ctttagaaag tccttatatc cccgaccaag
     2341 ctccaaaatc tagggataaa tggaaagagc aagtgcttga tttttggtct tggctagtgg
     2401 aagcgatcaa atctcctaca agtaagttgg aaacaagtat cacacacagt tacacagcct
     2461 ttctcttgct cattctgttt tctacacctt cctttttctt tagtatctat cacatcaaac
     2521 atgcttacta tggacatata gcaagcatta acagtcgctt ccctgagcag ctagctcctt
     2581 taactctttt ttctatcatc tctatcctag tagcgacaac actcttcttc ttttcattcc
     2641 tcttgggtag tttcgttgtg agacgattta tccaccagga aaaggactgg acgctagaca
     2701 aggttctcca acaatatagt caactcttgg caattccaat cttcctcact gctattgcta
     2761 gtttctttgc cttctttgat agcctacgat ttacagctct cttgtgtgtg attagcattg
     2821 gaatcattct gcttgccagt ctccatatca ttacaagacc aagtcaagca agtgaaaccg
     2881 actccttcta tcaattattc ttgtctgtcc ttgtgaacgg agttattatc ctcctcttct
     2941 ttgtagctga agtggcactg attggagatt atcttcgtgt cttggccttt ctttaaaatt
     3001 caatcctgtc atccttggca ggattttttc tatgtcaaaa aagcaagtcg attgacctgc
     3061 ttctgcttta ctcttcttgt gctaatgttt taaaatcttg tactaggatg ggttgtattt
     3121 ctaattgatt ggcgtctagc tgggggtaaa aagatggggg taacacctct aagaattttg
     3181 catagtccaa gcgagctatg gattcatagt cttctggttt agagccgtct cctgtgattt
     3241 gaaccaagtc aatctcccac tggacttcct tatccaccaa ttgctcaata taggctgcag
     3301 gaacaaacgg ttctctcggt aaaactgtct tgactccttg agctagatgg taaacgccat
     3361 gcacttttcc ttctccatcc tgatagaagg aaactctcgc aaagtaggca tctgtctctt
     3421 gaaccgcacg cacacgcgct tcaaactgag ccaagccatc tgcctctaaa aagcttttca
     3481 agttctcacg actaaagaaa ataggattaa aaattccttg gcaaatcgta aaacgagcta
     3541 cagggtctgc tagataggct tgaatacttg cttggaaata agcttcaaaa tcaaaaccat
     3601 ctagcccttc aatatcagtc cctgtataag caagcaaagc atctaaaaat gacttgataa
     3661 tctgccagtc tgtctttgtt agtaggtcag gaagatcgac acggtaagcc ttttgaccat
     3721 cagcataact gaccttaaaa agaaattgtg attgccccac tatcgcacac tcaatatact
     3781 cgagacgatt aagaggctga cggagataga ctacatcata gctatgtgac tccaaaccgt
     3841 ctagcaaggc caagatcgac ttggcagtca aaatctcttg ttctcctaaa atgctctgtt
     3901 tatttggaat aaaaaatgtt ttcgccataa ctggactcct ttgaaatcgt ttacatacag
     3961 tataccttat ttttctgaaa gatacaaaaa caaactttat attcttcgaa aatctcttca
     4021 aaccgcgtca gcttcgcctt gccgtagata tatgtaactg acttcgtcag tcttatctac
     4081 aacctcaaag cagtgctttg agcagcctgc agctagcttc ctagtttgct ctttgatttt
     4141 cattgagtat tagatttttg agtacaaagc atagaaaaac agcccctaag gactgtttga
     4201 tcttatacag tagctgcctg atccaagctt tcaccgatag cggctaggcg ctcgatcact
     4261 tcagcttgtg tcaattcatt ttttgaaaca tagcggttac gtgggtgaac acggcactcg
     4321 tgtgagcatc cacgaaggta cttgtcttca ttttcttctg atgtcaagat acgacggtta
     4381 cagaagggat ttccacagtt gacataacgt tcacatggtg ttccatcaaa ccagtctttc
     4441 cctacgatag ttgggttgac atggttgaca tcaacggcaa tacgctcgtc aaagacgtac
     4501 attttcccat cccaaagctc accttgaact tctgggtctt taccgtaagt tgcgattcct
     4561 ccgtgcaatt gaccgacatc tttgtagcct tcacggacca tccagcctga gaatttctca
     4621 cagcgaacgc cacctgtaca gtaaaccacg acacgcttgt ccatgaattt ttccttgtta
     4681 tcacggaccc attgtggtaa ctcacggaag ttgcgaatat ctgggcgaat agccccacgg
     4741 aaatgtccta ggtcgtactc ataatcgtta cgtgtgtcaa ggacaacggt atccttatca
     4801 agaagcgctt ctttgaactc ttttggagac aagtaagcac ctgttgtttc aagtgggttg
     4861 atgtcattgt caaagtcgtt gtcttccaaa ccaaggtgga caatttcttt cttgtagcga
     4921 acaaacatct tcttgaaggc ttgttcattt tcttcgtcaa tcttgaacca gagatcttcc
     4981 attcctggaa ggctgtgaac gtagtccatg tatttttgag ttgtttcata gtcacctgaa
     5041 actgttccgt taattccctc gtcagcgact aggatacggc ctttaaggcc gattgattta
     5101 cagaaagcca agtggtctgc agcaaattgc tctgcatttt caattggagt ataaaggtag
     5161 taaagtaaga cacgaatatc ttttgccata agatttgttc tcttttctat tcttaaattt
     5221 tctaaatttt ttatttatcc atactagtat acctgcttga gaaaacgaat gcaatttatt
     5281 tgactggaaa ttgtagaggg ttctcgaaat ttcctgaatg gttaaaataa ggacaagaga
     5341 aaacatggat atctatatcc ttgtgccaaa aaaaccactg ccctccccag accaacctga
     5401 ggaaagcagt gattcttatt ttaggagtta ggaatgaata cacgaaatca atttagctga
     5461 ttattttttg tttttcaaga attcatcgta ttgtttttgc atttcgttca atactttttc
     5521 gtaggcacct tcagatttca atttttccat caattctgga atcgctttat ctgggtctac
     5581 agtaccagtg ttgatagctg tatcaaattg ttgcattgtg ttagcaatag ctgagatttc
     5641 agattttaca ttgtcagtat tgaagataaa tccaagcgct ggagattctt tagcttctgc
     5701 caattctttc ttagaatttt cgatttgttg gtctgtaacg ttttcgttga tgtaaaggat
     5761 ccagttgtta ccagtgttcc atccacccat gtgagtgttt cctttgtagc catcaagaac
     5821 gcgaacacgg ttttctttac cttcaatttt ttcccagttc ttgccttctg gaccgtaaac
     5881 aagaccgttc aagagttctg gattcgtatt caagaggttc aagatttcca ttgatttttc
     5941 tttgttctta gagttgtttg agatgacaaa gttagcaact tgtgttgttt ggtttttctt
     6001 gatgaagtta gtaattggtt tgatttggat atctttgttg gcaacacgtg aaagcaagct
     6061 gttaccgtag tcagctggtc ctactgtttc ttcacgaacg aaccaagtat cttgttgaag
     6121 gtcaaaggaa gtatcgcttg ttgcgacgtc ttttggaatg tagccagctt catagaattt
     6181 gtgaagagtc ttcaagtgtt ctttgaaacg aggcacttcg taacggttta caactttagt
     6241 agtatcgcct tcaaggtcaa taacgaatgg aagaccgttt gctactgggt agtcaaagtt
     6301 atcagatggg atgaaaactt taccaatagc aaatggtact acgtctggag ctttttcttt
     6361 gatttgtttc aagactggct caagagtttc gtaagaagta acacctgaaa tatcgatacc
     6421 atatttggca aggagagttc cgttgaaggc aaagttttga gatgatgcaa cgttggctgc
     6481 aactggaaca gcgtaaattt taccgtttac agtattaccc ttgatataag ctgggtcaag
     6541 tgccttgtaa aggtctttac cttctttttt gtacaattct gtcaagtcag cataagcacc
     6601 tttttgagca tttacaatat agttatctgc aaaggcgata tcatagtttt caccagatga
     6661 tgtgataact gacattttct taccatagtc accccagcca aggtattgga tatccaattt
     6721 agcaccaact ttttcttcga tgattttgtt ggcatttgct aacaattcat ccaagttgtc
     6781 tggtttgtca ccaatttggt acattttgat aacaggtttg tcacctgaat cagcagcttt
     6841 tttgctgtta cctgtcaagt ttccacaagc agcaagacct gcaaccaaag cgactacact
     6901 agcagatgca aaagcatatt ttttccagtt tttcatgata aaaactcctt tttttatttt
     6961 taaacttata aacaatgtaa tgatcttata ctcaatgaaa atcaaagagc aaactagaaa
     7021 actagccgca ggctgctcaa agcactgctt tgaggttgta gataagactg acgaagtcag
     7081 ttacatatat ctacggcaag gcgacgttga cgcggtttga atttgatttt cgaagagtat
     7141 taacttcaca caagggaagt tgggaactga gaaatgttct ttttcaataa gcactattct
     7201 ttcacaccac cgatagtcaa accttttaca aagtagcgtt ggaagaatgg atacaaaatc
     7261 gcgattggaa gggttgctac cacaaccatg gccatacgac ctgtttcttt cggtagagca
     7321 actcccagtt gaccagtcaa gccgaccgct ttggcaatgt agtccatatt ttgttggatt
     7381 tgcatgagca aatattgcaa tggatacaag ttatcactct tgatgtaaag aagggcgttg
     7441 aaccagtcat tccagaaacc aagagctgtc aaaagcgtga tggttgcgat acctggtagt
     7501 gacaatggca aacagatttg gaagaaaatc cgagcctcac tggcaccatc gatacgagcc
     7561 gattcgagaa tggcttctgg aatggtcttc ttgaaaaaag aacgcatcaa gatgatattg
     7621 aatggtgaga gaagcattgg aacaatcaag gcccaaacag tgtcaccaag ctgaagtaca
     7681 cgggtcacca tgatataacc tggtaccaaa ccagcgttga acaacatact gagaaggacg
     7741 aagatggtaa aaaatctgcg atacttaaag gttgtccgtg aaatagcgta ggcataggtt
     7801 gttgtgataa agacatttgt caatgtccca actacggtta caaagacaga gatgaagagg
     7861 gcttgtagga ttttatcctt aaactgtgcc aaaaactcaa aaccgtctaa gccaaattgg
     7921 gatgggaaga agctatagcc gtattggagg aggcttttct cgtctgtcac tgaaataatg
     7981 ataacgaata caaaaggtag gatacaagag agggcaatca aacccgaaat gatactgaag
     8041 aagatatctg ctttcttact gaaggagtga atgccgacat tatcaatttt ttctttttta
     8101 attttctttt ttgccatatt ctcctccttt ctagaacaaa gctgagtttg gatcgactcg
     8161 tcttgcaagc aagtttgata ggataaccag aatcaaacca acaacggatt ggtaaagacc
     8221 ggctgctgca gccataccga tatctgctgt ctgagtcaaa ccattaaaga catatacgtc
     8281 caaaacgttg gttacattgt aaagctgacc agcattgtgt gggatttgat agaagagacc
     8341 gaagtctgcg cggaagatat ttccgactgc aaggatggtc aatacagtta caagcggagt
     8401 caactgagga atggttacgt tgcgaatacg ttgccacttg ctagctccgt ccactgtcgc
     8461 tgcttcgtag taggttggat caattcccat gatcgtcgca tagtacatga cactgctata
     8521 tccaaagcct ttccaaatac ctaggaaaag taggagatag ggccagatgc ccaggtcagc
     8581 gtagaaattg acttctttga gaccaagact ttccaataga tgattgaaca cccctttatc
     8641 aatatttagg aaggcatctg taaagaaact gatgataacc caagacaaga agtaagggaa
     8701 caacatagaa gtttgaaaaa tcttcaccat tctcttagaa cggagctcgc tgaggataat
     8761 ggcaatccct acagatacaa ctaaacctag aaagataaag ccaagattgt agaggacggt
     8821 atttcgtgtg ataataaagg cgtctcttga actaaataag aatctgaaat tatcgagtcc
     8881 gacccattta ctatttatga tactatctat gaaaccatta ctggtcatgt ggtagtcttt
     8941 gaaggcaacc acgttcccaa atactggaat gtaaaagaat agaatcaacc agagtgcccc
     9001 tggcaaaacc atcaagagaa agatccagtt gtctctcaat gtttttgaaa actttttcat
     9061 aatttcctcc ctttttattt tgatatccat ctaaaaattc ttttttagac ttttgataac
     9121 gattacatta ttagtatact cctatttgca ggttaggtta aactcctaat tatagaaaaa
     9181 actccacaaa ttatgtagca gatttaaaac tttatcacca ctatcaaaca aatgtcctaa
     9241 atcaattgtt tattttatct ctattagccc agtgatggcg tcactctgtt ataagcatcc
     9301 aacaacgggg tatactgaaa aatctccaga ctagggaact cagcgatagt tcctaatctg
     9361 gagattttta atatgttatt aggcgtttgc tttcaactta gcaataacct ctttaagatt
     9421 atcaatcacc tctgcttcag tatgctcaga gcctttttca tctgccaaga acaaaactgc
     9481 tttttgaagt tctttttgag agttttcaag gacatcctta tctactgttt caaggtttga
     9541 gtctttaaga agtttactta attccttggc taatttcttg agtttgattt gcagactcat
     9601 cttctcctgc tgtttctttg cccgctgttt gtcctccatc cttagttgct gactggcttt
     9661 ccttaatgga ctctagggaa gcaatggcat ctttgactgt ttgcaagata tcacgtaaac
     9721 cttgctctgt caaactatca tctgcaaaag ctttcttagc ctctgccaaa accagacgtg
     9781 ctgaatctgt ggtaggattc gatacacctg tcaatgatct caaaagattt tctaaggttt
     9841 gagtctgctt actaatacta gactaaaatc aaaaagcatt atataacagt gatatgaaat
     9901 caactaaaga agaaatccaa accatcaaaa cacttttaaa agactctcgt acagctaaat
     9961 atcataaacg ccttcaaatc gttctatagt aaaatgaaac aagaacagga caaatcgatc
    10021 aggaaagtca aatcgatttc taacaatgtt ttagaagtag aggtgtacta ttctagtttc
    10081 aatccattat atttcgtctg atgggcaaat cttataaaga gattatagag cttttatagt
    10141 gatttgaaat aagatgtgaa caactctatc aggaaagtca aattaattta tagaaatatt
    10201 ttagcagcca aggtgtactg ttataaattc aatacact
//
LOCUS       DAVGJL010000075        10211 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.075, whole genome shotgun sequence.
ACCESSION   DAVGJL010000075 DAVGJL010000000
VERSION     DAVGJL010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10211
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.075"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            397..819
                     /locus_tag="VVE42_001431"
     CDS             397..819
                     /locus_tag="VVE42_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU3073614.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            816..1046
                     /locus_tag="VVE42_001432"
     CDS             816..1046
                     /locus_tag="VVE42_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU3073615.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            1507..1710
                     /locus_tag="VVE42_001433"
     CDS             1507..1710
                     /locus_tag="VVE42_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEU3073616.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            1792..3009
                     /locus_tag="VVE42_001434"
     CDS             1792..3009
                     /locus_tag="VVE42_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU3073617.1"
                     /translation="MSEKRRDNKGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNE
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMK
                     RLNKEKQQERLVA"
     gene            3286..3699
                     /locus_tag="VVE42_001435"
     CDS             3286..3699
                     /locus_tag="VVE42_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073618.1"
                     /translation="MKKIIFIKTIELLAIDGIMLAFLTFKEGLTWDWILIYSGWLIFF
                     HPVLLAYLSNQLCDHFSHLYSQIRPKFWHFALQILLWDSLMILSLICLSGVPLLLQGT
                     LLILGHLISSYRISQRLKRDFPKAYQETISFWSIL"
     gene            complement(3738..4181)
                     /locus_tag="VVE42_001436"
     CDS             complement(3738..4181)
                     /locus_tag="VVE42_001436"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEU3073619.1"
                     /translation="MKIIIQRVTKAQVSIEGQVQGKINQGLLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGELLSISQFTLFADTKKGNRPAFTGAAKPDMA
                     SDFYDAFNQKLAQEVPVQTGIFGADMQVELVNDGPVTIILDTKNR"
     gene            complement(4211..6433)
                     /locus_tag="VVE42_001437"
     CDS             complement(4211..6433)
                     /locus_tag="VVE42_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEU3073620.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALVYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRVIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTDRHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHELWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKAMHEV
                     AEYGVAAHWAYKKGVKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IVTNPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEESLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEIKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYSSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
     gene            7406..8035
                     /locus_tag="VVE42_001438"
     CDS             7406..8035
                     /locus_tag="VVE42_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU3073621.1"
                     /translation="MKIHKTVNPVAYENTYYLEGEKHLIVVDPGSHWEAIHQTIEKIN
                     KPICAILLTHAHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFHFKVLPTPGHSIGGVSLVFPDAHLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGPATTIAHEKAFNPFF"
     gene            complement(8158..10050)
                     /gene="pepO"
                     /locus_tag="VVE42_001439"
     CDS             complement(8158..10050)
                     /gene="pepO"
                     /locus_tag="VVE42_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HEU3073622.1"
                     /translation="MTRYQDDFYDAINGEWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPVLPLLKEFQELETFADF
                     TAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVAAVVLSNEESSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEEAWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
BASE COUNT         3044 a         2185 c         2010 g         2972 t
ORIGIN      
        1 gccaataaaa aaaaccgtta tatggtagtg ctatttacgc tgttaaaata ttgtatatta
       61 cttccaaatg gcggtttgtt ggaggtcaac gtcgccatga agtacatcat atacaataaa
      121 tttccttaca ttgggttctt gtcaaaaaaa gtcgtctatc tgcaatagat aagtacgtcc
      181 accaatgtgg ttttataaat catatagata gaataacaga agcatgtaaa cagagaaata
      241 aatctgttta tatgcttttt tggctattca gaactttttt acaaagttta tttatcagta
      301 atgcaacaaa tccccctttc acattgggac taagagtgaa aggagataaa cgagcaaggc
      361 tcacttcctt tcctagacag aaagggggtg agaaacatga aaccatcttc ttttcagacc
      421 acaatagaaa atcagtttga ctatatctgt aaacgtgcta tggaagacga gcgaaagaat
      481 tatatgcttt atctttcaag gattgcaaag cgtgaggtgt ccttttcgga tgttggcgat
      541 tatcttgtta gccagtttgc gacaacagat aactattcaa ctgactttca gatttttaca
      601 ctcaatgggt tatcagtagg cgttgaaaat gatttgttga gtgaagcatt acgtgagttg
      661 ccagacaaga aacgtgaaat tctactgctg ttttacttta tggacatgag cgattcagaa
      721 attgcagacc tgttgaaatt gaaccgttct actgtctatc ggcatagaac cagtggacta
      781 gccttaatta aaaagtttat ggaggaattt gaagaatgaa aacacaatat cctatgattc
      841 cctttcctct cattgtaaag gcaacagatg gcgataccga agcgattaac cagattctac
      901 atcattacag agggtacata acgaagcgtt ccctacgact tatgaaagat gaatatggca
      961 atcaaagtat ggtcgttgat gaagtcttac gtggaagaat ggaaaccaga ctgattacaa
     1021 agattttgtc atttgaaatt aagtaatatc ctctctcctt tcgtggaagc gtgctaaacc
     1081 attccacgct tcccgaacag ggaggtttgt tattccacca aagcatattg agctttcaat
     1141 gtgttttgat aggctaacga gccattgttc tttgaaaact gaataaaagt aatcgaatac
     1201 gtttcgataa gaaaagagcc aacggaacta accgccatga cctatcttat aaagatagcg
     1261 agcgattcat gttagtgatc cgagaagcaa tctttagcag gattgcctgc aacgacattc
     1321 ttatcgtgat aatgatactc ccatacagtc aatagtccga gcgtgataaa accgtcgcag
     1381 gcaatgagta tggctacatg agaaccatgc aggggtggaa ctcccgtgag ctttgctaaa
     1441 gctgttcgat tgctggtaaa acaactttta tgaaatccaa ataagtgatt tggaaaggag
     1501 gattttatga agcagactga cattcctatt tgggaacgtt ataccctaac cattgaagaa
     1561 gcgtcaaaat attttcgtat tggcgaaaac aagctacgac gcttggcaga ggaaaataaa
     1621 aatgcaaatt ggctgattat gaatggcaat cgtattcaga ttaaacgaaa acaatttgaa
     1681 aaaattatag atacattgga cgcaatctag cgtcgccaaa gggtcttgta tatgataaaa
     1741 tagtattaag tcgtatcaag gctctttcca taaaggaaag gagcaaatgc catgtcagaa
     1801 aaaagacgtg acaataaagg tcgaatctta aagactggag agagccaacg aaaagacgga
     1861 agatacttat acaaatatat agattcattt ggagaaccgc aatttgttta ctcgtggaaa
     1921 cttgtggcta cagaccgagt accagcagga aagcgtgatt gtatctcact tagagagaaa
     1981 atcgcagagt tacagaaaga cattcatgat ggtattgatg ttgtaggaaa gaaaatgaca
     2041 ctctgccagc tttacgcaaa acagaacgct caaagaccaa aggttagaaa aaacactgaa
     2101 actggacgca aatatcttat ggatattttg aagaaagaca agttaggtgt aagaagtatt
     2161 gacagtatta agccatcaga cgctaaagaa tgggctatta gaatgagtga aaatggttat
     2221 gcttatcaaa ccatcaataa ctacaaacgt tctttaaagg cttcattcta tattgctata
     2281 caagatgatt gtgttcggaa gaatccattt gactttcaac tgaaagcagt tcttgatgat
     2341 gatactgtcc ctaagaccgt actaacagaa gaacaggaag aaaaactgtt agcctttgca
     2401 aaagctgata aaacctacag caaaaattat gatgaaattc tgatactctt aaaaacaggt
     2461 cttcgtattt cagagtttgg tggtttgaca cttccagatt tagattttga gaatcgtctt
     2521 gtcaatatag accatcagct attgagagat actgaaattg ggtactacat tgaaacacca
     2581 aagaccaaaa gtggcgaacg tcaagttcct atggttgaag aagcctatca agcatttaag
     2641 cgagtgttag cgaatcgaaa gaatgataag cgtgttgaga ttgatggata tagtgatttc
     2701 ctctttctta atagaaagaa ctatccaaaa gtggcaagtg attacaacgg catgatgaaa
     2761 ggtcttgtta agaaatacaa taagtataac gaggataaat tgccacacat cactccacat
     2821 agtttgcgac atacattctg taccaactat gcaaatgcag gaatgaatcc aaaggcatta
     2881 cagtacatta tgggacatgc taatatagcc atgacgctga actattacgc acatgcaaca
     2941 ttcgattctg caatggcaga aatgaaacgc ttgaataaag agaagcaaca ggagcgtctt
     3001 gttgcttagt agtacaaatg aatttactac ttatttacca cttctgacag ctaagacatg
     3061 aggaaatatg caaagaaacg tgaagtatct tcctacagta aaaatactcg aaagcacata
     3121 gaataaggct ttacgagcat ttaagaaaat ataaaaagat aattagaaat ttatactttg
     3181 tttcaaagaa aaaagatagt aaacgctttc acactccaac taaatattgt ataataatta
     3241 tattcaatat tacgtcacta acagatgaaa aaaggaggtt ttagcatgaa aaaaatcatc
     3301 ttcatcaaaa ccattgagct ccttgccatc gatggaatta tgctggcatt tttgacattt
     3361 aaagaggggc tcacttggga ttggatttta atttatagtg gttggctcat tttctttcac
     3421 cctgtgctat tggcctatct ttcaaaccag ctttgtgacc actttagtca cctctattcc
     3481 cagattagac caaaattctg gcattttgcc ttacaaatcc ttctatggga tagcctgatg
     3541 attctctcct tgatatgttt gagtggtgtg ccacttctcc ttcagggaac tctcctaatc
     3601 ctaggacatc tcatctcttc ctatcgcata agccaaagac tgaaaagaga cttcccaaaa
     3661 gcctatcaag aaacgatttc attttggagt attttatgat agatgagaaa aaccaagccg
     3721 actgggcttg gtctttctta tctattttta gtatctagga taatggtaac tggtccgtca
     3781 ttgaccagct caacttgcat atccgctcca aagatacctg tctgaacggg tacttcttgc
     3841 gctaattttt gattgaaagc gtcatagaag tctgatgcca tatcaggctt ggctgcacct
     3901 gtaaaggctg gacgattgcc tttcttagta tccgcaaaga gggtaaactg agaaatagaa
     3961 aggagttctc cttcaatatc tttgacagac aggttcatct tgccttctgc gtctgaaaaa
     4021 atccgcatat tgacaagctt tctcacagca tagtccaaat cttcctcttg gtcctctggt
     4081 ccaacaccaa ccagcaataa gaggccttgg ttgattttcc cctgcacctg accttcgata
     4141 cttacttggg cttttgtaac ccgttggata atgattttca taatagcctt tctagtaaga
     4201 gctagggcaa ttaaccgtta gtccgtttga cagaatagac ttctggcaca ctcttaatct
     4261 tatctacgac agtagtcagt gtagagaggt tggcaatgcc gaaggacaca tggatattag
     4321 caaatttcat atccttggtt ggttgggcgt tgaccgttga aatattcttg gttgtgtttg
     4381 aaagaacttg cagtacatcg ttcaataatc ctgtacggtt gaggccgtaa atatcgatat
     4441 gggccatata ctccttattt gagctagagt actggtcttc ccattccaca tcaaggagac
     4501 gttgctcata gttttcttgg gcacgcaggt tcatacagtc cacacggtga atagccacac
     4561 cacgcccctt ggtaatgtag ccaacaatat cgtcacccgg aacaggatta caacacttgg
     4621 caatccgcac taggagacca gaggctcctt caatgaccac accaccctca tgcttgacct
     4681 tgagggtttc tttgttttca accttgatct cgccaccttt gacaagttct tctgcttccg
     4741 ccttagcctt ggcacgctct tcttcacggc gttccttttc agtcagacgg ttaaagacgg
     4801 taatagcacc gatttctcca aaaccgatgg ccgcaaagag ggattcttct gtcttgtagc
     4861 tagtcttttg tagaacttgg tccatgtggc gcttgtccat gaatttattg gccacatagc
     4921 cattttcttg gaactgagcc atcagcattt cacgaccctt gttgacagac aattccttat
     4981 cttgattttt aaagaactga cgaatcttgt tacgagcctt gctggtcttg accatattga
     5041 gccaatcacg gctcggtcca aaggagttag ggttggtgac aatttcaacc tgatcccctg
     5101 tcttgagctt ggttgtcagt ggaaccatac ggccattgac cttggcaccc gtcgcttttt
     5161 caccgacttt ggtatgaatt tcgtaggcaa agtcaatcgg tcctgaatct ttgggaagag
     5221 aacggacagc tccatctggt gtaaagacgt aaatctcctc agccagatag ttttccttaa
     5281 cagagtccac aaattccttg gcatcatcag cttgatcttg aagctccatc atctccttga
     5341 tccagttcat cccaatagcc gattccttgc tgttaacctg ccccttaacg cctttcttat
     5401 aagcccagtg agccgcaacc ccgtactcag ccacctcgtg catagccttg gttcgaattt
     5461 ggaattcgat cggccctttt ggcccataaa cagtcgtatg gatagactga taaccattgg
     5521 ccttgcggtt agcaatatag tctttgaagc ggccaggcat cggtttccaa agttcatgca
     5581 cataaccaag catggcataa acatcacttt gggtatccaa aatacaacga atagcaatca
     5641 gatcatagat ttcctcaaac cgttttctct tatcctgcat tttgcggaaa atcgagtaaa
     5701 tatgcttagg acgaccgtaa atcttccctt tcaggtgacg atctgtcgta tattcctcta
     5761 attttgtgac tacctcatcc accaaggctt cacgctctct acgtttttcc ttcatcatat
     5821 gggtaatctt gtaaaactcc gttggattga gataacggaa agataggtct tccaattccc
     5881 atttgacact ggaaatcccc agacggtggg caagtggggc atagatttcc atggtttctt
     5941 tggaaatacg ctcctgcttg tcttttctaa ggtgtttcag ggtccgcata ttgtgcaagc
     6001 ggtcagacag tttgaccaag ataacacgga tgtcctcaga catggccatg agcatcttgc
     6061 gatgattttc cgctaattgc tcctcgatag atttgtactc gaccttccca agtttggtaa
     6121 ccccatctac gattacccgc acatcaggac caaactctct ttccaaatcg tccaaggtcg
     6181 catctgtatc ctccaccaca tcatgcaaga aaccgcaagc taccgttacg gcatccagct
     6241 tgagcttggc taaaatacct gccacttgga tagggtgaat aatataaggt tcgccagatt
     6301 tgcggtattg accactatgg cattccaccg catagaccaa ggccttatgg acaaaatgga
     6361 catcctcttc cgttaaatat tctttggtta aagcgacaac ttcttcgcct gttaaattca
     6421 cttctttcgg catctctact ctccaattct tcctaccatt ctatcacttt tttaagaata
     6481 tgaaaactag attggaacag aataagaaaa aaataattca aaattgcttg ataattctga
     6541 attattggcc cataatatac tacgaagtta gattttaaac ttaggtgata gaagaagaga
     6601 tagaagaacg gaaaccatat tgtaacccaa agactttctg acttccccaa ttccattgaa
     6661 gatacgaaag ataaacggtg gaactcgtat cccttgactg gattttggaa ttaatactca
     6721 atgaaaatca aagagcaaac taggaaacta gccgcaggtt gctcaaagca ctgctttgag
     6781 gttgtagata agactgacaa agtcagtcac atatatacgg caaggcgacg ttgacgcggt
     6841 ttgaagagat tttcgaagag tataaaaatc ctcaagatac tttcttctat cctttagttt
     6901 ataaggagaa tacctatgaa aaaaactgct atttctatct ttgctctcct aatgttagga
     6961 gtttgctgcc tgttcctatt cagccagcaa agctataaaa aacagtcgtt caatactatg
     7021 ctaacgacca gaacctgccc agtaggataa cttatagtga atatagcgac aaacgagaag
     7081 ccaactacgg tagcactcta aacatcacgt ctatcaaaca agctaatgac ggagtttatg
     7141 caacctatga agggcaattg acacctttcc aatattgatc cagcctgtct tcatctagtc
     7201 atgctgattt ttaagttcat tttaaatcct tacctattct ccctaactgt gctatactta
     7261 atttatactc aatgaaaatc aaagagcaaa ctagaaagct agccgcaggc tgttcaaagc
     7321 actgctttga ggttgcagat aaagttgacg cagtttgaag agattttcga agagtattag
     7381 tatattcttt gagattggag ctagtatgaa aatccataaa accgtgaatc ctgttgccta
     7441 tgaaaatacc tattatctag aaggcgaaaa gcacctcatc gtcgtcgatc ctggtagtca
     7501 ttgggaagcc attcatcaga caatcgagaa gatcaacaaa ccgatctgtg ctattctctt
     7561 gacccacgcc cattatgacc atatcatgag tctggacttg gttcgcgaga cgtttggcaa
     7621 tcctcctgtc tatatcgcag agagcgaagc cagctggctc tacactcctg tcgataatct
     7681 ctccggtctc cctcgccacg atgatatggc agatgtggtc acaaaacctg cagaacacac
     7741 ctttgtcttt cacgaagaat accaactaga ggaatttcat tttaaggttc taccgacccc
     7801 agggcactct atcggtggtg tttccctagt ctttcctgat gctcacctag tcttgacggg
     7861 agatgctcta ttccgcgaaa ctatcggacg gaccgacctt ccgactggta gcatggagca
     7921 actccttcat agtatccaga cccaactctt caccctacca aactacgatg tctatccagg
     7981 acatggtcca gctactacta tcgctcacga aaaggccttc aatccctttt tctagcaaga
     8041 tgatgacaat cgaaatttaa gtaaactatc cagcaaatct ttctattaca aaaggcatcc
     8101 tatcaaggtt ttcacacatg attggatgcc ttttttctga tgactagatt ttttgcatta
     8161 ccaaataatc acgcgctcct ctggtgaacg ccacattccg tctccttctt tgacatcata
     8221 ggttgtaaag aaatcgtcga agtttggtac ttgcacattg acacggagtt tggctggtgc
     8281 gtgcacatcg acgctagcca aaagtttcat aaattctggt cgacctttca tgcgccagat
     8341 gcgaccgaag ttgtagaaga actcttctgc tgagaagtct gcttctctct tagctgcttc
     8401 aagcgctgct gcgattcctc ccaagtcagc cacgttttct gatacagtca atttaccgtt
     8461 aatggttgct ccataagaat cctgtccatc aaattggtca atgacttttt gtgttttctc
     8521 cttgaaggca gcatagtcgc tctctgtcca ccaatccttg aggctaccat tttcgtcaaa
     8581 ggaagccccg ttagtatcaa aggcgtggga aatttcatgg gcaatcactg ccccaatacc
     8641 accgtagtta gcagaagatg actgatgcaa gtcatagaaa ggcgcctgta aaatggccgc
     8701 tggaaagaca atcaggttct tctgaggatt gtagtaggca ttgaccatat gagcaggcat
     8761 gccccattcc ttatagtcta caggctggtt ccacttactc caactgtgct tgatttccac
     8821 acgcgcaaag gctagagcat tctcaaaaag actggcagtt tcattcacta ccttatcctt
     8881 gtaacgtgca ggcaattctt ctggatagcc aatataaggt ttgatcacat tgagcttcac
     8941 gatagcctgt ttacaggttt ctggagtgag ccagtcattc ttaagcagac gctccttata
     9001 gacatcaatc atggttgcca cttttttctc cacatccgcc ttggcttctg gagagaactt
     9061 ctcacgggcg taccaaagcc ccagggcttg cttgaaaggt tcctgtgcta gatgataagc
     9121 tgctttgacc ttatcttttg cctctggaac tccagaaagg gcacggctgt aggcaccaga
     9181 caaaacacgg atatcctctg ttaaatagct ggttgaaaga ttgacaacac tcaaaatcaa
     9241 ggttgcttta aggagagacc aggcttcctc actgtaaaat tgctctgctg cttgccagaa
     9301 acgttcctcg tctacaataa ccttgtctgg taattgccca ataactgctt tgaagaagtc
     9361 atccaaaggt agggcaggcg cgaatttctt gaaatcttcg taagaatatg gatgatagag
     9421 tttagcatat tctgaacttt cttcattaga gagcaccact gccgcaactc ggcggtccaa
     9481 ttcaagtctt ttttctagca agtcttcaat ttcttcatca gagaaatcat aagccttgag
     9541 gagatttgcg ctactttctt tccaaagagt caagagctct tcgcgctgag gatgttcttc
     9601 tgcatagtag gtcgtatctg gcaagattgt gcttggagcg ctagcccata gaacattgat
     9661 tctagcatcc ataaagtctg gcgatacacc aaaaggaagg aagtttggtt ttcctgcaag
     9721 ctcaaactct gctagtttag ctgtaaaatc cgcaaaagtt tccaattctt ggaattcttt
     9781 aaggagtggt aagacaggtg tgataccgtc agcttctctc ttgtcaaaat cacgaactag
     9841 gcggtggtat ttgacaaagt tttccaagat agcatcctca ggcacttctt cacctgctaa
     9901 ccacttgtct gttgtcgcca gcatcaggtc ttcaatttcc tggtctaaat caacaaaacc
     9961 tcctgtttga gacttatctg ctgggatttc agctgtctgt tgccattctc cattgatagc
    10021 atcataaaaa tcatcttgat aacgtgtcat cttgttctcg ctttcatttg tatttgcctt
    10081 tatcttaaca aaaatcacta gggaatgcaa ttaacaacca tttaactatt attttttagc
    10141 tattataaaa aacctttgca tgataaaacg cataattcca agttttttac acttgatact
    10201 atgcgtttta t
//
LOCUS       DAVGJL010000076        10102 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.076, whole genome shotgun sequence.
ACCESSION   DAVGJL010000076 DAVGJL010000000
VERSION     DAVGJL010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10102
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.076"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(312..1622)
                     /locus_tag="VVE42_001440"
     CDS             complement(312..1622)
                     /locus_tag="VVE42_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEU3073623.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNVAITNGTLDVLAQHILYRTRTHPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGRTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYQKTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTTIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            1859..2731
                     /locus_tag="VVE42_001441"
     CDS             1859..2731
                     /locus_tag="VVE42_001441"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEU3073624.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAILLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
     gene            2862..3694
                     /locus_tag="VVE42_001442"
                     /pseudo
     CDS             2862..3694
                     /locus_tag="VVE42_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            3755..4717
                     /locus_tag="VVE42_001443"
     CDS             3755..4717
                     /locus_tag="VVE42_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU3073625.1"
                     /translation="MNMSNITIDGQEMDLSQTMPSYAQVVSLYDDTSISVSNNETDKV
                     LAGSLCTDTNEQGLTIPSSLLKNWNEQTGKNLTANDLIGKSVSASIVESAAETSKIAQ
                     FQTKIVRVINDEDDMEDSNSFMLSHQMETISKEAGFTKAVSYFILELKDPSQTKVVTE
                     ELQKNKKYTVLSQQRVLDIVITFIRVIQGLLIVLSSQAIVVAAVMIGIIIYINIMQRS
                     KEIGVMKAVGYQNRDVKGIFIYEAIWIVGIALLLAFLVAQGVGSLANAIVSHFYPSIT
                     KVFELNLLSVLGTLVFALLLGYVSAYFPARKISKMDPVESLRYE"
     gene            4894..5901
                     /gene="gap"
                     /locus_tag="VVE42_001444"
     CDS             4894..5901
                     /gene="gap"
                     /locus_tag="VVE42_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEU3073626.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFVKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHDVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(5968..6804)
                     /locus_tag="VVE42_001445"
     CDS             complement(5968..6804)
                     /locus_tag="VVE42_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU3073627.1"
                     /translation="MTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVHWNEIVNPGD
                     VCQLTFDEEDYSQKTIPWGNPDLVQEVYQDQHLIIVNKPEGMKTHGNQPNEIALLNHV
                     STYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISREYWALVDGNINRK
                     ELVFRDKIGRDRHDRRKRIVDAKNGQYAETHVSRLKQFSNKTSLARCKLKTGRTHQIR
                     VHLSHHNLPILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLEKLTFTTLSNTFEKELKK
                     NG"
     gene            6930..9125
                     /locus_tag="VVE42_001446"
     CDS             6930..9125
                     /locus_tag="VVE42_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEU3073628.1"
                     /translation="MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYI
                     DKNQETEAAEVDMTSQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQIVYENISLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEAGWALNKQLDNHTMQYDSYKVDNYAGI
                     KTSREVPMYQALAESLNLPAVATVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIASHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYIMAGKTGTTEAVFNPEYTSDQWVIGYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTSGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
     gene            9178..9330
                     /gene="rpmG"
                     /locus_tag="VVE42_001447"
     CDS             9178..9330
                     /gene="rpmG"
                     /locus_tag="VVE42_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU3073629.1"
                     /translation="MALKKASLACAVCGSRNYSIKISGNPKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            9340..9516
                     /gene="secE"
                     /locus_tag="VVE42_001448"
     CDS             9340..9516
                     /gene="secE"
                     /locus_tag="VVE42_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEU3073630.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            9571..>10102
                     /gene="nusG"
                     /locus_tag="VVE42_001449"
     CDS             9571..>10102
                     /gene="nusG"
                     /locus_tag="VVE42_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEU3073631.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAE"
BASE COUNT         3138 a         1768 c         2208 g         2988 t
ORIGIN      
        1 tttgactatc ctgatctatt tgtcctgttc ttatttcatt tcactatatc tcaaattgag
       61 tatgacgaag tgcgctccca tgtcctggga acgcactttc ttcatatttt tcatattctt
      121 gaatccatcg ataaagacta ttgggatgaa tttttaaagt tgaactaatc atttttacag
      181 gatgagattt acagcagaga gtttgaaggc tttatcaaag gtttttcttg gcataatgac
      241 ttttcctcgt ttccacttaa ttttgtgtct actttattat accaagtcca cccttaagtt
      301 agataataaa tctaacttaa ggaagctaga aggatgagaa tccaggtggt caagagtccc
      361 aaacttaagc tgatggggac acccagaata atttgctttt tgaaggcaag gccacgttcc
      421 tctatattgg gaagtgagag ttgaatgaga gaaccagctg atgaaaaggg tgagatatta
      481 gtagatagag cgccaataac ggtggctgtt gtgagtaagt gaatatcaat ctgaggattt
      541 tgagcactga tgatagcaat gatgggaaag agggctggag ctacaacgga tagggtggaa
      601 ctaaagagtg acatcactcc ggctatcaca caaaagaaca gaggtaacca gaaatgagga
      661 atggttgttg tcatgaggtg ccctatcagt gtgactaaac ctgacttgac cgctagagac
      721 attagtaagc tcatgccgca gagcatgata attgtagccc agggaacctt agctaaaatg
      781 gcttcttgct tccctaattt gagccttaag gcgaggcaga ccatgagtat tgagacaaag
      841 ccaatatcaa atgttttttg ataagtagct atccaggcga tgtttgggaa aatgagatgc
      901 aacaagggaa aaagccaaac caaaaccatg ctgctgatca tgagcaaggt ggtttgtctt
      961 tgaaccttgc tgaggagtgg tggttggtca atagtcaagg atgagtttgt tcttccctta
     1021 ctatagtgac tgtaacagga taataaaagc aagacgatga gtgggtagat aatgctgacg
     1081 ataaagatat gattgccaag tgaaaaagct tgctcttccc atcccatttg cttaaacagg
     1141 ccttgaaaga caatgcctga gctactggtt atcaaattag cccctcctga agctccccaa
     1201 ttgacggctt gagctccaat caaagggtgt ttgtccgctt tttgacagag ggtaatcgct
     1261 agaggacagc aaacggccat agtagtgaaa aatccagcac ctaaagcaga caaaagggtt
     1321 gccatcaggt ataaaatcat gtagagggcg ttagggtggg tgcgtgtgcg gtagagaatg
     1381 tgttgagcca aaacatcaag agtaccgtta gttattgcaa cgttataaaa gagagagacg
     1441 ctaaaaatgg taaaaaagag tgaggttggc caaaaatgaa gaagttcttt ggggcttaat
     1501 cccatgagag tggttgcgat gaggtaagaa aaagcaatag ccagcaggcc aatattgatt
     1561 ttggtgcggt aaccaattcc aatagctaga gcaatggcgc taatcattat taaatgaatc
     1621 attgaattgt cctttcagtt agaatatgga aataggttgg caattttggc gctcaaaaga
     1681 ccacctgttt cctattggta cttgactatt atacacaaaa aaaatacaat tgtcttgaca
     1741 attgtattga cacttgttag aattaaaaat gaggtattca ggcttgtgat ttcctatgga
     1801 agttaatagt gattgcctct aatgcttaca agtgatatta aaaatagagg acctagtgat
     1861 gtcaatcatt tcaactgatt taaccccttt tcaaatagat gatacattga aagcagcctt
     1921 gcgagaagat gttcattccg aagattacag taccaatgcc atttttgatc atcatggcca
     1981 agccaaggtg tcgctttttg ccaaggaagc tggtgtttta gcggggctaa ccgtttttca
     2041 aagggttttt accctatttg atgccgaggt gaccttccag aatcctcatc aatttaagga
     2101 tggggatcgt ttgactagtg gcgatttggt tttagaaatc ataggctcgg tgagaagtct
     2161 cttaacatgt gaacgcgttg ccttgaattt tttacaacat ttatcaggga ttgcttcgat
     2221 gacagctgct tatgtagaag ccttaggcga tgattgcatt aaggtatttg atactcgaaa
     2281 aactactcct aatttacgtc tttttgagaa atatgccgtg agagttggcg gtggctataa
     2341 tcatcgcttt aatttatcag atgctatcct gctaaaagac aatcacattg cggcagtagg
     2401 tagtgttcaa aaggcaattg ctcaagcgcg tgcctatgcc ccttttgtga aaatggtcga
     2461 ggtggaagtg gaaagccttg ctgctgccga agaagctgcg gcggcgggtg ctgatattat
     2521 catgttggat aatatgtcat tggaacagat tgaacaggcc attaccctaa ttgcaggacg
     2581 ttctcggatt gaatgttctg gaaatattga tatgaccact attagccgtt ttcgtggttt
     2641 agcgattgat tacgtctcca gtggtagttt aacccatagt gctaagagtc ttgatttttc
     2701 catgaagggt ttaacctacc ttgatgtcta agttgtaaaa taaactaact ttttaaagga
     2761 tgtctttcct ctagaacgag ttttatgtca gatagtttaa gcgcctcttc aaatatagta
     2821 aaatgaacca aaaatagtac acaatgtggt ataatcttct tatggcatat tcaatagatt
     2881 ttcgtaaaaa agttctctct tattgtgagc gaacaggtag tataacagaa gcatcacacg
     2941 ttttccaaat ctcacgtaat accatttatg gctggttaaa gctaaaagag aaaacaggag
     3001 agctaaacta ccaagtaaaa ggaacaaaac caagaaaggt tgatagagat agacttaaaa
     3061 actatcttac tgacaatcca gacgcttatt tgactgaaat agcttctgaa tttggctgtc
     3121 atccaactac catccactat gcgctcaaag ctatgggcta cactcgaaaa aaagaaccac
     3181 acctactatg aacaagaccc agaaaaagta gccttatttc ttaagaattt taatagttta
     3241 aagcacctag cacctgttta gattgacgaa acaggatttg atacttattt ttatcgagaa
     3301 tatggtcgct ctttaaaagg tcagttaaga agaagatatc agaggatttc tttggttgca
     3361 ggtctaacaa atgaatggtg agttaatcgc tccaatgact tacgaagaga cgatgacgag
     3421 cgactttttt gaagcatggt ttcagaagtt tctcttacca acattaacca caccatcggt
     3481 tattattatg gataatgcaa gattccatag aatgggtaag ttagaactct tgtgtgaaga
     3541 gtttgggcat aaacttttac ctcttcctcc ctactcacct gagtacaatc ctattgagaa
     3601 aacatgggct catatcaaaa agcacctcaa aaaggtatta ccaagttgca atacctttta
     3661 cgaggctttt ttatcctgct cttgtttcaa ttgactatag ttcacggata cagttgggaa
     3721 agaagctatt aaatatttgg agagtccttt tgggatgaac atgtcaaata ttactataga
     3781 tgggcaagaa atggatctga gtcaaacgat gccttcttat gctcaagtag tgagtcttta
     3841 tgatgataca agtatctctg ttagtaataa tgagacggac aaagtgttgg ctggctccct
     3901 ctgtactgat acaaatgaac agggattaac gattccaagc agtttactaa aaaattggaa
     3961 tgaacagaca ggaaaaaatt tgacagctaa tgatcttatt ggcaaatcag tctcagccag
     4021 cattgtagaa agtgctgccg aaactagtaa gattgctcaa tttcaaacga agattgtacg
     4081 tgtaatcaat gatgaagatg acatggagga cagcaacagt ttcatgctgt ctcatcaaat
     4141 ggaaacgatt tcgaaagagg ctggatttac gaaagctgta tcttatttta tcttggaact
     4201 caaagatcca tcacagacaa aagtagtaac agaagaatta cagaaaaata agaagtatac
     4261 tgtgctttct caacagagag ttcttgatat tgtgattacc tttattcgtg ttattcaggg
     4321 attattgatt gtgctttcat cacaagctat tgtggtagca gcggttatga ttggtatcat
     4381 tatttacatc aatatcatgc aacgttccaa ggaaataggt gtcatgaagg cagttggcta
     4441 tcagaatcgt gatgtcaaag gaatttttat ttacgaggct atctggattg taggcatcgc
     4501 cttgctgctg gcatttttgg ttgcacaagg ggtgggaagt ttggcgaatg cgattgtaag
     4561 tcacttttac ccatccatca ctaaggtttt tgaattaaat cttttatctg ttttaggaac
     4621 tctagttttc gctctattac ttggttatgt ctcagcctac ttcccggcgc gtaagattag
     4681 taaaatggat cctgtagaat cgttacgcta tgaatagtaa gggattcctt gatttacagt
     4741 cttaaaaaga aactaacata gaaaaacaat ggccatactt gcaattcaac tcaaatagtt
     4801 gtataatagg tttgttgaaa ggtgatttgt agtgattcaa gttactcttt tcacaataaa
     4861 aatttcatga ttttcataag gaggaaatca ctaatggtag ttaaagttgg tattaacggt
     4921 ttcggacgta tcggtcgtct tgctttccgt cgtatccaaa acgtagaagg tgttgaagtt
     4981 acacgcatca acgaccttac agatccagtt atgcttgcac acttgttgaa atacgacaca
     5041 actcaaggtc gtttcgacgg tactgttgaa gttaaagaag gtggattcga agttaacggt
     5101 aaattcgtta aagtttctgc tgaacgtgat ccagagcaaa tcgactgggc tactgacggt
     5161 gtagaaatcg ttcttgaagc tactggtttc tttgctaaga aagaagcagc tgaaaaacac
     5221 cttaaaggtg gagctaaaaa agttgttatc actgctcctg gtggaaacga cgttaaaaca
     5281 gttgtattca acactaacca cgacgttctt gacggtactg aaacagttat ctcaggtgct
     5341 tcatgtacta caaactgctt ggctccaatg gctaaagctc ttcaagataa ctttggtgtt
     5401 gttgaaggat tgatgactac tatccacgct tacactggtg accaaatgat ccttgacgga
     5461 ccacaccgtg gtggtgacct tcgccgtgct cgcgctggtg ctgcaaacat cgttcctaac
     5521 tcaactggtg ctgctaaagc tatcggtctt gtaatcccag aattgaacgg taaacttgac
     5581 ggatctgcac aacgcgttcc aactccaact ggatcagtta ctgaattggt agcagttctt
     5641 gaaaagaacg ttactgttga tgaagtgaac gcagctatga aagcagcttc aaacgaatca
     5701 tacggttaca cagaagatcc aatcgtatct tcagatatcg taggtatgtc ttacggttca
     5761 ttgtttgacg caactcaaac taaagttctt gacgttgacg gtaaacaatt ggttaaagtt
     5821 gtatcatggt acgacaacga aatgtcatac actgcacaac ttgttcgtac tcttgaatac
     5881 ttcgcaaaaa ttgctaaata attcttgagt tgatagaaag caaggctttg tggtcttgct
     5941 ttttatatgg aaaaatggat gacacgatca tccattcttt tttaattctt tttcaaatgt
     6001 atttgaaagg gtagtgaaag ttagcttttc taaagtaagt gggtgggtaa aggaaagtcg
     6061 gaaggcatga agcataagcc gacttgtctt tgatttacta ttatagagag ggtcgcccag
     6121 gataggaaga ttatgatgcg aaaggtgcac acgaatctga tgggttcgcc ctgtctttag
     6181 cttgcaacga gccaaggaag tcttgtttga gaattgcttt aatctgctta catgcgtttc
     6241 agcatattgc ccattttttg catcaactat tctttttcta cgatcatggc gatcacgtcc
     6301 aattttgtct ctgaaaacaa gttcttttct gttgatattt ccatcaacta gagcccaata
     6361 ttctctagaa atctcttttt tctccaataa gcgattgaga atgggcagga taaaaggatt
     6421 tttggcaaag agaactaagc cactggtttc catgtccaga cgatgaacga catagcaggt
     6481 ttggccaaca taggtactga catggttaag aagggcaatt tcgtttggtt gattaccatg
     6541 cgttttcatc ccctctggtt tgtttacaat aatcaagtgt tgatcttgat aaacttcctg
     6601 cactaagtct gggttgcccc aagggatcgt cttttgggaa taatcttcct cgtcaaaagt
     6661 caactggcaa acatctccag gatttacgat ttcgttccag tggacttctt cttgatttat
     6721 caaaatatgt ttcttgattc tcaaaaaatg acggattttt ctagggatga ggagttgttc
     6781 ctcaagtaat tgctttaccg tcatttgagg tagagaggcg ggtaatgtaa atgtgaattg
     6841 catacagata ttgtaacaaa aaaagcccta tttggatagg aaatagctaa attcttgtct
     6901 tcctatgatg aagatgataa aataaacgca tgaaattaga taaattattt gagaaatttc
     6961 tttctctttt taaaaaagaa acaagtgaac tagaggactc tgattctact atcttacgtc
     7021 gctctcgtag tgatcgaaaa aaattagccc aagtaggtcc gattcgaaaa ttctggcgtc
     7081 gttatcatct aacaaagatt atccttatac taggtttgag tgcaggattg ctagttggaa
     7141 tctatttgtt tgctgtagcc aagtcgacca atgtcaatga tttgcaaaat gccttgaaaa
     7201 ctcggactct tatttttgac cgtgaagaaa aagaggctgg tgccttgtct ggtcaaaagg
     7261 gaacctatgt tgagctgact gacatcagta aaaacttgca gaatgctgtt attgcgacag
     7321 aagaccgttc tttctataaa aatgacggga ttaactatgg ccgtttcttc ttggctattg
     7381 tcactgctgg acgttcaggt ggtggctcta ccattaccca acagctggct aaaaacgcct
     7441 atttatcgca ggatcaaact gttgagagaa aagcgaaaga atttttcctt gccttagaat
     7501 taagcaaaaa atatagtaag gagcaaattc taaccatgta ccttaacaac gcttattttg
     7561 gaaatggtgt gtggggtgta gaagatgcga gtaagaaata ctttggagtt tctgcatcag
     7621 aagtgagtct ggatcaagct gcgactctgg cagggatgct caaggggccg gaactgtata
     7681 atcccttgaa ttctgtagaa gattctacta atcggcgcga tactgtcttg cagaatatgg
     7741 ttgcagcagg atatattgat aaaaaccaag aaaccgaagc tgctgaagtt gatatgactt
     7801 cgcaattgca cgataagtat gaaggaaaaa tctcagatta ccgttacccc tcttattttg
     7861 atgcggtggt taatgaagct gtttccaagt ataatctaac agaggaagag attgtcaata
     7921 atggctaccg catttacaca gagctggacc aaaactacca agcaaatatg cagattgttt
     7981 atgaaaacat atcgctattt ccgagggcag aggatggaac gtttgctcaa tcaggaagtg
     8041 tagctctcga accgaaaaca gggggagttc gtggagttgt cggtcaagtt gctgacaatg
     8101 ataaaactgg attccggaat ttcaactatg caacccaatc aaagcgtagt cctggttcta
     8161 caattaagcc tttagttgtt tatacgccag cagttgaagc aggctgggct ttgaataagc
     8221 agttggataa ccataccatg cagtatgaca gctataaggt tgataactat gcagggatca
     8281 aaacgagtcg agaagttcct atgtatcaag ccttggcaga atcgcttaat ctacctgctg
     8341 ttgccactgt taatgatttg ggtgtcgaca aggcttttga ggcaggcgaa aaattcggac
     8401 tcaacatgga aaaggttgac cgtgttcttg gtgtcgcctt gggaagcggt gttgaaacca
     8461 accctcttca aatggctcaa gcatacgccg cctttgcaaa tgaaggttta atgcctgaag
     8521 ctcattttat tagtagaatt gaaaatgcta gtggacaagt tattgcgagt cataaaaatt
     8581 cacaaaaacg ggtgattgat aagtctgtag ctgacaagat gaccagtatg atgttgggga
     8641 ctttcaccaa cggtaccggt attagttcat cgcctgcaga ctatattatg gcagggaaaa
     8701 ctggaacaac tgaagcagtt tttaatccgg agtacacaag tgaccagtgg gtaattggtt
     8761 atactccgga tgtagtgatt agccactggc ttggctttcc gaccactgat gaaaaccact
     8821 atctagctgg ctctacttca aacggtgcag ctcatgtctt tagaaacatt gccaatacca
     8881 ttttacctta tacgtcagga agtaccttta cggttgaaaa tgcttataag caaaatggaa
     8941 ttgcaccagc caatacaaaa agacaagtac aaaccaatga taatagccag acagatgata
     9001 atttgtctga tattcgaggg cgtgcgcaaa gtctagtaga tgaggctagc cgggctatct
     9061 cagatgcgaa gattaaggaa aaggctcaaa caatatggga ttcgatagtc aatctatttc
     9121 gctaagatgc ttgtcaaagc ctagctttct tgttataatg gataagatgg aggcgttatg
     9181 gcactaaaaa aagcaagcct agcttgtgcg gtttgtggtt cgagaaacta ttcaatcaag
     9241 atcagcggaa accccaagcc tacacgacta gaagtaaata aattttgtaa gcattgtggc
     9301 aagtacacta cacatagaga aacgagatag gagagagcga tgcgttttat tggagatatt
     9361 tttagacttc ttaaagacac aacgtggcca actcgcaagg aaagctggag agattttcgt
     9421 tctatcatgg aatacacagc tttctttgta gtaattattt acatttttga ccagttgatt
     9481 gtttcaggtt tgattcgatt tattaacatt ttttagaaga ttagtggagt taattacact
     9541 agaaatcttc tatttatgaa aggaaatatc atggatagtt ttgataaagg atggtttgtt
     9601 ttacaaactt attctggtta tgaaaataag gtgaaagaaa atctattaca acgtgcacaa
     9661 acctacaata tgttggataa tattctacgc gttgaaattc caacacaaac agtgcaagtt
     9721 gaaaaaaatg gaaagagaaa agaagtagaa gaaaatcgct ttccaggtta tgttcttgta
     9781 gaaatggtca tgacagatga agcttggttt gttgttcgaa acacaccaaa tgttacagga
     9841 tttgtcggat cacacgggaa cagatcaaaa ccaactccat tattggaaca agaaattcgt
     9901 gacattttgg tatctatggg acaaactgtt caagaatttg atttcgatgt tgagattggt
     9961 caaactgtac gtattattga tggtgctttt gcagactaca ctggtaagat tacagaaatt
    10021 gataataaca aagtgaaaat gattatctct atgtttggta atgacacagt tgcagaagta
    10081 aacctaaacc aaatcgcaga at
//
LOCUS       DAVGJL010000077         9750 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.077, whole genome shotgun sequence.
ACCESSION   DAVGJL010000077 DAVGJL010000000
VERSION     DAVGJL010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9750
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.077"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(10..1416)
                     /gene="lacG"
                     /locus_tag="VVE42_001450"
     CDS             complement(10..1416)
                     /gene="lacG"
                     /locus_tag="VVE42_001450"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEU3073632.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTGYGQVNAKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHACAVKLYKEKGYKGEIGVVHAL
                     PTKYPLDPENPADVRAAELEDIIHNKFILDATYLGRYSAETMEGVNHILSVNGGSLDL
                     REEDFTALEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGEKGSSKYQIKGIGRR
                     VAPDYVSRTDWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKQHLEILSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKVAETQIID"
     gene            complement(1631..3325)
                     /locus_tag="VVE42_001451"
     CDS             complement(1631..3325)
                     /locus_tag="VVE42_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEU3073633.1"
                     /translation="MDKLIIFIEKGKPFFEKLSRNIYLRAIKDGFISSMPAVLFSSIF
                     ILIAAVPNIFGFKWSDEQLAFILKPYNYSMGILALLVAGTTAKSLTDSVNTRSMEKTN
                     QINYMSTFLAAVVGLLILAADPIEGGFANGLLGTRGLLTAFLAAFITVNIYKVCIKNN
                     VTIRLPEEVPPNIAQVFKDVIPFALSVLSIYGLDLIVRNIFGTNVAESVGKILAPLFS
                     ATDGYIGLAIVFGAYAFFWFVGIHGPSVVEPLIVAISYANIEANVQLVQAGMHADKIL
                     NPVTQTFVVTMGGTGATLVVPFMFMWLCKSKRNRIVGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFIPFVTAPIINVWIMKFFVDVLQMNSFSIILPWTTPAPIGIVMGTALAPLSF
                     VLAITLIIIDTLIYYPFVKVYDHQILEEERKGNSSSELKEKVAANFNTAKADAILEKA
                     GVDAAQNTITEETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGGYGAHREMLP
                     EFDLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQEQFD"
     gene            complement(3327..3644)
                     /locus_tag="VVE42_001452"
     CDS             complement(3327..3644)
                     /locus_tag="VVE42_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU3073634.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKAAEAGDFEKADALVE
                     EAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVK"
     gene            complement(3680..4516)
                     /locus_tag="VVE42_001453"
     CDS             complement(3680..4516)
                     /locus_tag="VVE42_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEU3073635.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEEVIVIGKGIAFGKKKGDLIAENQ
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALTQGRYKDSNLPDISAKYPVAFQIANEAFEIYRQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLTDYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGSKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
     gene            complement(4750..5730)
                     /gene="lacD"
                     /locus_tag="VVE42_001454"
     CDS             complement(4750..5730)
                     /gene="lacD"
                     /locus_tag="VVE42_001454"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEU3073636.1"
                     /translation="MALTEQKRVRLEKLSDENGIISALAFDQRGALKRLMAQHQTEEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAAFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGETAAREWLRTTGFENIDELNKVLQRTATSWKERV"
     gene            complement(5732..6661)
                     /locus_tag="VVE42_001455"
     CDS             complement(5732..6661)
                     /locus_tag="VVE42_001455"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEU3073637.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGKLGEFLVEHIDNQVKKDFFSIQGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQEFLEHFKKLLESVEVVAISGSLPAGLPVDYYASLVELANQAGKPVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLDELKEVLQEPLFAGIEWI
                     IVSLGANGTFAKHGDTFYKVDIPRIQVVNPVGSGDSTVAGISSGLLHKESDAELLIKA
                     NVLGMLNAQEKMTGHVNMANYQVLYDQLIVKEV"
     gene            complement(6672..7187)
                     /gene="lacB"
                     /locus_tag="VVE42_001456"
     CDS             complement(6672..7187)
                     /gene="lacB"
                     /locus_tag="VVE42_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEU3073638.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPSEENKKLIAKIEHLESHNAQQTDANFFTEF
                     LEKWDRGEYHD"
     gene            complement(7218..7643)
                     /gene="lacA"
                     /locus_tag="VVE42_001457"
     CDS             complement(7218..7643)
                     /gene="lacA"
                     /locus_tag="VVE42_001457"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEU3073639.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMG"
     gene            complement(7802..8266)
                     /locus_tag="VVE42_001458"
     CDS             complement(7802..8266)
                     /locus_tag="VVE42_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HEU3073640.1"
                     /translation="MGGHPGFNCPLLDDEVYEDYYLEFEKEEICSVPRPFPETGMLDF
                     QDRSPWLEGQKEIDLSYDLFSTDAVTLDELQSRTIALRSLKHDKGLKVHFAEFPNLII
                     WSTLNKGPFITFEPWSGLSTFLEEGDHLEDKKNVCLLEANQVEELGFEIEVL"
     gene            complement(8315..9056)
                     /locus_tag="VVE42_001459"
                     /pseudo
     CDS             complement(8315..9056)
                     /locus_tag="VVE42_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(9064..9366)
                     /locus_tag="VVE42_001460"
     CDS             complement(9064..9366)
                     /locus_tag="VVE42_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU3073641.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPAMSAPVFDNIRLPAKQNMV"
     gene            complement(9404..>9750)
                     /locus_tag="VVE42_001461"
     CDS             complement(9404..>9750)
                     /locus_tag="VVE42_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU3073642.1"
                     /translation="LITREKSFPTGLDMEFLGKDLPNVAIPHTDIVHNLAEKVVVVRL
                     EKPVTFHNMIAPDKEVEVSLLFFIINNSSSSQTNILAQLMDFFTGNGHLEDLSKISEP
                     EKLYAYIAEATA"
BASE COUNT         2910 a         2016 c         1686 g         3138 t
ORIGIN      
        1 ctaattctac tagtctataa tctgagtttc cgctactttc ttgtaccagt gagctgattt
       61 cttaggataa cgttcttgag tttcaaaatc tacgtagaag agaccataac gtttctcata
      121 accgtttgac catgagaaga catccattaa tgaccaaatg aagtaacctt ttacattagc
      181 tccatctgca atcgcatcag acaagatctc caagtgttgt ttcacgtaat caatacgacc
      241 atcatcgtaa acagtgttat caacgaactc atctttatag ccgagaccat tttcagtgat
      301 gtaaatcttc ttgtagttag gataatcttt cttcacacgc atgatttggt catacaaacc
      361 ttgagggtag ataatccaat cccaatccgt gcgtgataca tagtcaggag ctacacgacg
      421 accaatacct ttgatttgat acttagagct tcctttttca cctttaccat tatggataat
      481 ttcagtttct ccatcaaagg cttccatcca gtcactcata tagtagttga ttcctaggaa
      541 atcattcaag tcttttgcag cttctaatgc tgtaaaatct tcttcacgaa gatccaaact
      601 accaccattg actgataaga tatggttgac accttccatg gtttcagctg aatagcgacc
      661 tagataagtt gcgtctaaga tgaatttatt gtgaatgata tcttccaact ctgctgcacg
      721 aacatctgct ggattttcag gatctagagg atatttagta ggcagggcgt gaacaacacc
      781 aatttcccct ttatagcctt tctctttgta caattttacc gcgcatgcat gagacaccat
      841 catattgtgg tgtgattgaa agactttggc aaggtcgtac tggatacctg gagggaattt
      901 cccaaccaaa tattgaccat caccgattgg tccaatttca ttgaaggttg tccaatagtt
      961 tacttctgga aattcttcaa aacagaaggc agcgtagtct acaaaatgtt cgatattttc
     1021 acggtttaag aagtctccat ttgagtggag agcttctggc gtgtcaaagt gatgaagagt
     1081 tacaaagggc tcaacatgac gtttgtgaca ctctgcaaat aaattatgat aaaactcaac
     1141 acctttagca tttacttgac cataaccagt cgggaaaata cgtgaccagg caatagaaat
     1201 tcgaatgcca ttgacaccat actcttctgc tagcttgaga tcaactggat atcgattgta
     1261 aaaatcactg gctggttctg cagtgtacca gtagttatct tcaagatatt tatcccacgc
     1321 tactggtcct ttaccatcag tatgtgtagc accttctgct tgataggcag ctgttgcgcc
     1381 accaaaaata aagtcttttg gaagtgtttt tgtcattttt ttcacctatt tcttgattta
     1441 aataaagtaa aagtgagagg agaggagtca gtgagccatc cttctccatc tcacttcttg
     1501 taccaagtca ccgaattgtg acatgaggag gtaaaaacga tatcttttta ccgacgaatt
     1561 aataaggcga ttaggaaatt cgccatagcg atttccgtaa cgataggaga ctgtttcaca
     1621 gttcctagcc ttaatcgaat tgttcttgta cgaatgctag ggcacctttt ccatcacgag
     1681 ttaatttgat gtattgagcg ccttctgttt tcgctagttt aataccgagc ttatctgttt
     1741 ctgccttcat atcttcaaag tttgaagcaa cttgaggagc aaggataacc aaatcaaact
     1801 ctggtaacat ttcacggtga gcaccatagc cgcctgctgc tgctttcaca gggacattgt
     1861 attctgctgc tgccttattc aaagcatttg caaggagtcc acttgtacct ccaccagcac
     1921 agagaacgag gacatttgtt tcttcagtga tggtattttg tgctgcatcg acacctgctt
     1981 tttcaagaat agcatccgct tttgcagtat tgaagtttgc agcaactttt tctttcaatt
     2041 cagatgaaga gtttcctttc cgttcttctt ctaaaatttg atggtcataa actttaacaa
     2101 atggataata gattaaagtg tcgataatga ttaaagttat agccaagacg aatgacaatg
     2161 gagccagcgc cgttcccata acaataccaa ttggagcagg tgttgtccaa ggtaaaataa
     2221 tactgaaact attcatttgt agaacatcta caaaaaattt cataatccaa acattgataa
     2281 taggagcagt aacaaacgga ataaagaaga taggatttaa aacaattgga gcaccaaata
     2341 ggattggttc atttacgcca aagaaagtag gaactacaga cgcacgtccg acaattctat
     2401 ttctttttga tttacataac cacataaaca taaatggaac tactagtgtt gcaccagtac
     2461 cacccattgt tacaacaaaa gtttgagtaa ctggatttaa aattttatct gcgtgcatac
     2521 cagcttgcac aagctgaaca ttagcttcta tatttgcata actgatagct acaattaaag
     2581 gttctacaac agatggccca tgaattccaa caaaccaaaa gaaagcataa gcaccaaata
     2641 caatagctaa accgatataa ccatcagtag ctgaaaatag aggggctaat attttaccaa
     2701 cagattctgc tacatttgta ccaaaaatat ttctaacaat taaatccaat ccatatattg
     2761 aaagaacaga taaggcaaac ggaataacat ctttaaaaac ttgtgcaata tttggtggaa
     2821 cctcttcagg aagtctaatg gttacattat ttttaataca tactttatag atatttactg
     2881 taataaaagc tgccaaaaat gcagtaagta atcctctagt acctaaaaga ccattagcaa
     2941 atccaccttc aattggatca gctgccagta ttaacaatcc aacaacagct gctaaaaaag
     3001 ttgacatgta gttgatttgg ttggttttct ccatactacg tgtattaact gaatctgtta
     3061 acgattttgc agttgtccca gctactaaga gagctaaaat tcccattgag tagttgtaag
     3121 gtttcaaaat aaaagctaac tgttcatcag accatttaaa cccaaaaata ttgggcacag
     3181 cagctattag tataaaaata cttgagaata atactgctgg catactggaa ataaaaccat
     3241 cttttatcgc tcttaagtat atatttctcg ataatttttc aaagaaaggc tttcctttct
     3301 caataaaaat tattaactta tccattttat ttaactcctc tcttgtaaag ttcaattaaa
     3361 tggtgcatta ggtcttttaa caagatagtt gtcatcaagt gatcttgacc atgcatcatg
     3421 gtcacactat acgctaagtc ctcaccagaa gcttccttag tcaatagact tgtttgcgcg
     3481 tggtgagcct ctgcaataca gctaccagct tcctctacca gagcgtccgc tttttcaaaa
     3541 tcaccagctt cagcagcctt caaggcttcc aatagttttg aacgagcatc gccagcatag
     3601 gctacgattt caaaacctaa caatgttact tcttctctat tcatgataga attctcctta
     3661 tatagtttta aataaatttt tatgacaata aacgttggat atgtagaact agataaactc
     3721 gttcactttt atacaaatca agacccgtat gttgcgtaat cacatcatag atcttggaac
     3781 caatctcgaa ggcttttgga taggattgtt taatatgatc ttccatatcc agaagtgatt
     3841 ggttatcatc tctagatctg tctaaataat ccaagaaata attcaaatgg atcataaagc
     3901 gatcatagaa atggttattc tttttagttc gttgaattgc atagtccgtt aaaacttctt
     3961 caacattcct gagaatttct ttcctcttat caatcgactc cacaagttcc acttcatttt
     4021 caccttcagc attaatgaaa tgataagcaa tccgaataat ttcgtcctca ggaaaatgat
     4081 ctgctagctt ctgacggtaa atttcaaaag cttcatttgc gatttgaaaa gcgacaggat
     4141 acttagcgga aatatctggc agattactat ccttgtacct tccttgagtt agagcttgat
     4201 aagaacagta aatatgatct gtcaaggtta catagagata ctcttgaatc ggataatgat
     4261 atttctttga tagcttatca atgatttcat aggtcactgt gataaaatca agcggaacat
     4321 ctttgagaag agccataaag ttttctctgg actcttcggt cttcatccga aagattttct
     4381 caacctgatt ttcagcaatc aaatccccct tcttctttcc gaatgcaatc cccttaccaa
     4441 ttacaatcac ctcttctccc ttatcatttc tgacaagcga gacattgtga ttcattggat
     4501 ttagaattcg atacatgtca acctcctttt atatcttaaa ggtatatgag tacaaaaaat
     4561 gaccacaaat gaactagaaa atcagctgat ttctaattca cctgtgatca tgcctaatat
     4621 tacttagtaa cactcacctt gtattaactt gtgatttaag tatagcacaa gtaagttatt
     4681 ttgtaaacct ttacatacaa ctttttttaa atttttttag attcatgttc ctaaactagg
     4741 aggactttct tacacacgtt ctttccatga agttgctgtt ctttgaagaa ctttgttgag
     4801 ctcatcaatg ttttcaaatc cagttgtgcg aagccattcg cgagctgctg tttcaccatc
     4861 tttgatgtat gcttcaactg atccagccca tgttgcacgg ccacaaagaa ctccgttaaa
     4921 gtttgcacct gattcatgag caaatacaag agtatcttgg aagagtttag ctgatacacc
     4981 agcactcaag tagatgtatg gcaagttcgt tgcttcatct tgcgctttga agaaggctgc
     5041 tgcttcttca cgtgtataaa ctacttcacc ttcagcgaag ccttcaacat atttaatgtt
     5101 aacaggaact tcaactttca agacatcaat gttaaagcgt gggtctgaaa agactttcat
     5161 agcgccgata actttgtgtg gttttacttt cgcgtattct acagaacctg catccgcaat
     5221 tttttcatcg taagcaagaa tttcaaggaa gaatgggata tcttcagcca cacactcaga
     5281 accgatgcgt tcgatgtagg cttgtttttc ttgattgagt tcgtctgagc tatctacatc
     5341 atagtaaagc aagaatttaa ctgcatctgc accttcttct ttaatacgtt ttgcagacca
     5401 gacatccaag cagtctggca agcgttttgt gcttgttgtg tcataacctg ttttttcata
     5461 agcaaggaga agaccagctt tttcatcaag agctttagtt gctggaagtc catactcagg
     5521 gtcaagaagc atagatgaag cgtatttagt caattcatct gctaccaaga ctttaagttc
     5581 ttccatttgg gccacagttg gttcttctgt ttggtgttga gccatgaggc gtttcaaagc
     5641 accacgttgg tcaaatgcaa gagctgagat gataccattt tcatcagaaa gtttttctaa
     5701 gcgtacacgt ttttgttctg ttaaagccat tttatacctc ttttactatt aattgatcat
     5761 atagaacttg atagttggcc atgttgacat gaccagtcat tttttcttga gcattgagca
     5821 taccaaggac atttgccttg atgagtaatt ctgcatccga ttctttgtga agaagtcctg
     5881 aagaaattcc tgccacagta gagtctccag atccaacagg atttaccacc tgaattctag
     5941 gaatatctac cttgtagaaa gtgtcaccat gtttggcaaa agtaccgttg gcaccaagtg
     6001 aaacgataat ccattcaatc cctgcaaaca aaggttcttg aagtacttct tttaattcat
     6061 ccaaatcctc agaaacttct cttccaagaa gctgagacaa ttcttcatta tttggtttga
     6121 tgactgttgg tttatggggt gattcaagaa cagcctgaag tgctgcacct gagcagtcca
     6181 agactacagg cttgccagct tgattagcaa gttctaccaa gctcgcatag taatcaactg
     6241 gaaggccagc tggcagacta cctgagatgg ccactacttc aactgactcc aagagttttt
     6301 tgaaatgttc caaaaactct tgaccttcct gttccaatac ttcaggacct ttttcaagaa
     6361 cttctgtttg gttgtctccg tggagaatag cgatacagtt acgagtttct ccctgaattg
     6421 agaagaaatc tttctttact tgattatcga tatgttcaac caaaaactca ccaagtttac
     6481 cacccactaa accagtagca agaacagaat cgccaaattc tgaaagtact cgggtaacat
     6541 tgagtccctt accaccagcc gttttggtta catccaccac acgattgaca gtatcaatct
     6601 tcaactcatc caagggatag gaaatatcaa tggatgggtt cattgtgact gttaaaatca
     6661 taggtcacct cttagtcgtg gtattctccg cgatcccatt tttcaaggaa ttctgtaaag
     6721 aagtttgcgt ctgtttgttg agcattgtga ctttcaaggt gttcaatttt cgcaatcaat
     6781 tttttgtttt cttcagatgg tttgtattca gcatgaatga aagcttcgat gatatcacac
     6841 ataagcaatt caccagtaat cttaccacca aaaccgataa cgttggcgtt caattgttct
     6901 ttagcataaa gagctgttgt catatcacga accaaggcag aacgaacacc tggaacttta
     6961 tttacagcgt tgttgatacc aacaccagta ccacagatac ataccccgag atcagcttga
     7021 ccactagtta ctgcttcccc tactttttta ccaaagattg ggtagtgagt ccgtgtatgg
     7081 tcataggtac caaagtcaat gacttcatat ccttttgatt tcaaaaattc tgaaaccgcc
     7141 attttttcat cagttacgat gtggtcacat ccaattgcaa ttctcatttt taacttacct
     7201 ttcttactat aatctaatta gcccattttg ttcaacatgt caacccggat ttggtgacga
     7261 ccaccgtcgt atttaccatt aacaaatcct ttagcgatat ttttagccaa ttcatcacca
     7321 acaagttgtg cccccatagt gatcatacgt gagttgttgt ggccacgagt catataagct
     7381 gaacgttcgt cagatacttc tgcagcaacc attcctttga tcttagttgc aaccataaat
     7441 ggaccagctc cataagcatc aatcacgata ccaaggtttt gttcttcttt gtttacttct
     7501 gcagcaacag cgagagtcac atcaacaaag tcttgacctt cagctgtaac atccacaagg
     7561 tggaagtttt ctttttctaa gaagtctttc acaacttctt tcaatctcaa acctgcagca
     7621 tctgcaccga taacaataga catattgtat actccttttt attttttcta tgctggattc
     7681 cagcagtgta ttggctgtct ttaaaaaaac agtcaattag tcctcacaaa cataatatac
     7741 tactattgtt tgaagaagtc aatagtttgt gtttgttttt gttatttatt ttttactttt
     7801 tttataaaac ttctatctca aaccctaatt cttcaacctg attggcttct aagagacaaa
     7861 cattcttctt atcttctaaa tgatctcctt cttcaagaaa tgttgacaag ccagaccatg
     7921 gttcaaaggt aatgaaaggt cccttgttca aagttgacca gatgatgagg tttggaaatt
     7981 ctgcaaaatg cactttcaat cccttatcat gtttaagaga acgaagggca attgttcgag
     8041 attgcaattc atccaaagtc actgcatctg tgctgaaaag atcataacta agatctattt
     8101 ctttttgtcc ctctagccat ggacttctat cttggaaatc caacatacct gtttctggga
     8161 aaggacgtgg aacagagcaa atctcttctt tctcaaactc tagataataa tcttcataga
     8221 cttcatcatc aagtagagga caattaaatc ctggatgtcc accaataaag taaggcctga
     8281 ccttgctagt ttctttatta gacttcctgc aaaactagaa tcctagttca tgattgataa
     8341 taccagcaat caaattcatt cgtaatccga agcgtttacg atgatttcga taggttgttg
     8401 aaaacatttt aaacgttttt actttggcaa agatgttctc aaccttgctt ctctccttgg
     8461 atagcgcatg gttacaggct ttatcttcag ctgttagcgg cttgagtttg ctggatttac
     8521 gtggagtttg tacttgagga tatatcttca tgagtcctta ataaccactg tcagccaaga
     8581 ttttaccagc ttgtccgata tttctgcgac tcattttgaa caacttcata tcatggcaat
     8641 agaaacaatt ctcccttgac ttgtgacaat cgcctgagcc ttcatagcgt ggcatttctt
     8701 tttacttccg tcacatcaat cattatcgtg ttctcagaac tgagaggagt tcttgaaatc
     8761 gtaacaccac tttgaacaag agttacttca acccattggc tccgacggat aaagttgctt
     8821 tcgtggatac caaaaacagc cgcaatttct tcataagttc gatattctcg cacatattga
     8881 agagtggcca taagaaggtc ttctaggctt aatttaggtt ttcgtccacc ttttgcgtgt
     8941 ttaagttgat aagctgtttt taatacagct aacatctctt caaaagtggt acgctgaaca
     9001 ccaacaagac gcttaaatcg tgcatcagtt agttgtttac ttgcttcatc attcatagaa
     9061 ctactatacc atattttgtt tcgcaggaag tctaatattg tcaaatactg gagcgctcat
     9121 tgctgggata cggaataaga ttggtccagc ttcgataact gggatacctg gttcaaaacc
     9181 aaggtctgtt gcagcgattg gtgtaaagat atcgtaacct ttcataaggt cttcgtttac
     9241 atctttcacc ataactgcat cacagtgaac atcgtaacca cggtttgaaa gttcttcttc
     9301 tagagcactt ttaatttggt gacttgagtt aacacctgca ccgcaggcag caagaatttt
     9361 aatcatttgg atttcctccg attttatttt ttaatagaca agattaagcg gttgcttcag
     9421 caatgtaagc ataaagtttt tctggttcgg aaatttttga tagatcttca agatgtccat
     9481 ttcctgtgaa aaagtccatc aactgagcca gaatatttgt ttgacttgaa cttgagttat
     9541 tgatgataaa gaagagcaag gatacttcta cttccttatc aggagctatc atattgtgaa
     9601 aagttactgg tttttctaat cgaacaacca ccactttttc agctagatta tgaacaatat
     9661 ctgtatgagg aatcgctaca tttggcaagt cctttcctag aaattccata tctaaaccag
     9721 ttggaaatga cttttcacgc gtgatcaagg
//
LOCUS       DAVGJL010000078         9715 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.078, whole genome shotgun sequence.
ACCESSION   DAVGJL010000078 DAVGJL010000000
VERSION     DAVGJL010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9715
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.078"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..134
                     /locus_tag="VVE42_001462"
     CDS             <1..134
                     /locus_tag="VVE42_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU3073643.1"
                     /translation="LPPYSPEYNPIEKTWAHIKKHLKKVLPSCNTFYEAFLSCSCFN"
     gene            complement(618..1922)
                     /gene="eno"
                     /locus_tag="VVE42_001463"
     CDS             complement(618..1922)
                     /gene="eno"
                     /locus_tag="VVE42_001463"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEU3073644.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
     gene            2086..2532
                     /locus_tag="VVE42_001464"
     CDS             2086..2532
                     /locus_tag="VVE42_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEU3073645.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAEPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SPFGLVIHSNAPVQVEEKDLRLAFPKLWEVKKEEPAKTSLWKKWFS"
     gene            complement(2529..3332)
                     /locus_tag="VVE42_001465"
     CDS             complement(2529..3332)
                     /locus_tag="VVE42_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEU3073646.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLKEKWIQVTDPSGQKEAMRYFQKGELALFEVADLVGLGKIPLEKR
                     NPLQIQTCGIGELILHLISKGIKDIYIGVGGTASNDGGLGIAAGLCAFAQASIVSGID
                     TCLDLINFDKKVSDADLVVVGEGRLDSQSFAGKAPIGVAKRTPVGVPVIAICGSLAED
                     LPPLPFENIQAVFSILEKSEPLEDSLKNASLYLERTAANIGHLLNMCKI"
     gene            complement(3341..3986)
                     /gene="serB"
                     /locus_tag="VVE42_001466"
                     /pseudo
     CDS             complement(3341..3986)
                     /gene="serB"
                     /locus_tag="VVE42_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084485.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
     gene            complement(4045..5478)
                     /gene="glgA"
                     /locus_tag="VVE42_001467"
     CDS             complement(4045..5478)
                     /gene="glgA"
                     /locus_tag="VVE42_001467"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEU3073647.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGNQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYRWIQAYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGFERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDQILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWFAQIYPDKLSTNITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            complement(5475..6614)
                     /gene="glgD"
                     /locus_tag="VVE42_001468"
     CDS             complement(5475..6614)
                     /gene="glgD"
                     /locus_tag="VVE42_001468"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HEU3073648.1"
                     /translation="MKIDKYSAILGNTVGFHNMSTLTDHRPVASLPFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTLLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLAKKDIS
                     EVNAILDVDETDHVLSHKLFDSKSTAETFNMSTDIFVVDTPWLIEHLEEEAKKEHPEK
                     LRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQKIYTK
                     VKNEEPTYYANTSKVSTSQFASGSIIEGQVANSVLSRNIHVHRDSLVKDSLLFPRVVI
                     GEGAQVEYAILDKGVEVEPGVVIRGTAEHPVVVKKGAKVTEDIHS"
     gene            complement(6604..7746)
                     /locus_tag="VVE42_001469"
     CDS             complement(6604..7746)
                     /locus_tag="VVE42_001469"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEU3073649.1"
                     /translation="MKNEMLALILAGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGINSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVPLKEA
                     SRFGIMNTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLESGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGTVKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVATDED"
     gene            complement(7736..9664)
                     /gene="glgB"
                     /locus_tag="VVE42_001470"
     CDS             complement(7736..9664)
                     /gene="glgB"
                     /locus_tag="VVE42_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEU3073650.1"
                     /translation="MDNREALKTFMTGENFYLQHYLGAHREELNGEHGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPF
                     AVRYEARPGTGAILTELPEKKWKDGLWLARRKRWGFEERPVNIYEVHAGSWKRNSDGS
                     PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTHNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDVYHLDGIRVDAVSNMLYLDYDDAPWTPNKDGGNLNYEGYY
                     FLQRLNEVIKLEYPDVMMIAEESSSATKITGMKEIGGLGFDYKWNMGWMNDILRFYEE
                     DPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNL
                     YTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNLEDPMNAKMKYFTSQLNQFYKDH
                     RCLWEIDTSYDGIEIIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKDFTIGLPV
                     AGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRL
                     KSTKTVTNKNQKGVENEK"
BASE COUNT         2931 a         2174 c         1719 g         2891 t
ORIGIN      
        1 ctcttcctcc ctactcacct gagtacaatc ctattgagaa aacatgggct catatcaaaa
       61 agcacctcaa aaaggtatta ccaagttgca atacctttta cgaggctttt ttatcctgct
      121 cttgtttcaa ttgactatat aaattgtcta agcgaaacaa ccgataagaa ttggcacaaa
      181 agcgaccgta tttttgttac caatacagga aaaccagttc atagttctat cttgagcaag
      241 tctctccagc gagcaaacga acgccttaaa aaaccaattc ccaaacatct gtcccctcac
      301 atcttcagac acaccactat tagcatctta tcagaaaata aaattccttt aaaaacaatc
      361 acggacaggg ttggtcattc cgactctgaa gtcactactt ccatctacac acacgtcaca
      421 aagaacatga aagatgaagc aatcaatgta ctggataaag ttatgaaaaa gattttttaa
      481 aaagttttgc cccttttttg tcctctaaat acaaaaatag cccttcggat aaaatccgag
      541 gggctagaaa cgttgttaaa tcaacggccg aacttttgaa cttcatggtt cgggacaaaa
      601 tagttcactg aactatttta ttttttaagg ttatagaatg atttcaatcc acgatattca
      661 gctacttcac caagttggtc ttcgatacga agcaattggt tgtatttagc gatgcggtct
      721 gtacgtgaaa gtgaaccagt cttgatttgt cctgcgttag ttgcaactgc aatatcagcg
      781 attgttgaat cttcagtttc acctgaacgg tgtgatacaa cagcagtgta accagcttct
      841 ttagccattt cgatagcttc aaaagtttca gtaagagtac cgatttggtt aactttgata
      901 aggattgagt tagcagcacc ttcttggata ccacgtgcaa ggtagtcagt gtttgttacg
      961 aagaagtcgt caccaacaag ttgtactttc ttaccaagac gttcagtaag agctttccaa
     1021 ccatcccagt cgttttcatc cataccatct tcaatagtga tgattgggta tttgttaacc
     1081 aattcttcaa ggtagtcgat ttgttctgca gatgtacgaa cagcagcacc ttcaccttca
     1141 aatttagtgt agtcgtaaac tttacgttct ttatcgtaga attctgatga agcacagtca
     1201 aatccgataa atacgtcttt acctggtaca tatccagcag cttcaatcgc agcaaggata
     1261 gtttcaacac catcttcagt tccttcgaaa cgaggagcga atccaccttc gtcacctacg
     1321 gcagtttcca aaccacgtga tttaaggatt ttcttaagag cgtggaagat ttcagcaccg
     1381 taacgaaggg cttctttaaa tgttggcgca ccaactggca agatcatgaa ctcttggaaa
     1441 gcgattggag cgtcagagtg agaaccaccg ttgatgatgt tcatcattgg agttggaaga
     1501 actttagtgt tgaatccacc aaggtagctg taaagtggga tttcaaggta gtcagcagca
     1561 gcacgagcta cagcgataga cacaccgagg attgcattcg cacccaattt acctttgtta
     1621 ggagtaccgt caagtgcgat catagcacgg tcaatagctt gttgatcacg tacatcgtag
     1681 ccaatgatag cttcagcaat gatgttgttt acgttgtcaa cagctttttg tgtaccaaga
     1741 ccaccgtaac gagatttgtc accgtcgcga agttcaactg cttcgtgttc accagtagaa
     1801 gctcctgatg gaaccatacc acgtccgaaa gcacctgatt cagtgtaaac ttctacttca
     1861 agtgttgggt taccgcgtga gtctaggact tcgcgagcgt aaacatcagt aataattgac
     1921 attttttact ctccttatga gttaaatttt ttacacctct ataatacctt aaaacccctc
     1981 ctttttcaag aaaaaacgtt atctttgtgc aacttttcct taactttata aagtaatcgc
     2041 tttcttttgt ctgttttatt ccaactttta tgatatactg ttttcatgac agatttatca
     2101 aaacaattac ttgaaaaagc tcatggtggg ttaaaaataa atccggatga gcaaagacgc
     2161 tatcttggta cttttgagga aagagttctt ggatatgtag atattgacac agcaaatagc
     2221 cctcagttag aaaaaggctt tttatttatt ttagaaaacc ttcaggaaaa agcagagcca
     2281 ctatttgtga agatttcacc aactatcgaa tttgataagc aagttttcta cttaaaagaa
     2341 gcaaaagaaa ctgatagtca agccaccata gtatctgaag agcatattac ttctcctttt
     2401 ggcctggtta ttcatagcaa tgcaccagtt caagtagaag aaaaagacct tcgacttgct
     2461 tttccaaaac tttgggaagt taaaaaggaa gaaccagcca aaacatcctt atggaagaaa
     2521 tggtttagct aaatcttgca catatttaat aagtgcccaa tattggcagc cgtgcgctcc
     2581 agatagagac tggcattttt caaactatct tctaaaggtt cacttttctc caaaatagaa
     2641 aagacagctt ggatattttc aaatggtagg ggaggtaaat cttcagcaag actaccgcaa
     2701 atagcaataa caggaactcc aacaggggtt ctttttgcaa cacctatagg cgctttccca
     2761 gcaaagcttt gactatcaag tcttccttct ccaacaacaa ccaagtcagc atctgaaact
     2821 ttcttatcaa agttgattaa gtccaagcag gtatcaattc cagacacgat acttgcctga
     2881 gcaaaggcac acaaaccagc agcaatcccc agtcctccat cattactggc cgtgccacca
     2941 acaccgatat aaatatcttt aatcccttta gaaatgagat ggagaatcaa ctctccaata
     3001 ccacaagttt ggatttgaag tggatttcgt ttctctagcg gaatttttcc aagaccaacc
     3061 aagtcagcta cttcaaatag tgccagttcc cctttttgaa aatagcgcat ggcttctttt
     3121 tgtccagaag ggtctgtcac ttggatccat ttttctttta ggtcaagaga atgtcggata
     3181 gcatctacag taccttctcc cccatcacca acagggcaga ggagacattc tacatctgct
     3241 atcgattgtt ggaagcctct ttttattgct tcagctacct gttgagctgt caagctttcc
     3301 ttaaacgaat ccggtgcaat tacaatcttc atattttccc tcattctaaa cagtcaatca
     3361 aaggaagaac ttctaaaaaa tccctcttgt caacatgatg tggtatttct tttttgagca
     3421 cttctttggc acaaaaggcg attcctaact ccgccgactt caacattaat agattattaa
     3481 ccccatcacc gattgccacc cttctttctt tagaaagttt tagtttcttt ctccattttt
     3541 ccagagtctc ttttttgacc tggggactta taatttgtcc aactaatttt cctgttaaaa
     3601 gaccttcttt gacttcaagc tggttggcag tgaaataggc aataccaagg gattttgcta
     3661 atctctccaa ctattggtgt aaatccacca gacaccagac caactaggat gccattcttt
     3721 tggagaatag agatgaactc tgggacattt agcgatagat gaattgagtt gaagacgtta
     3781 tcaaagacca aaataggaag accttccaac aaggacactc tttttcttaa actgctttca
     3841 aagaccaact ctcctcgcat tgcccgactt gtaatctgcg aaatttccgc ctcatgacct
     3901 gcctctctcc ctaaaagatc aatcacttat tctaggatta aggttccatc tacatccaaa
     3961 acacacaagc cttttacttg agacatcagt tctcctctct aaacagccta aaaatcgtat
     4021 gaagtcatca tacgatttta tctattaatt aactaaacta tggtacaagt caaggtatga
     4081 cttgcaggct gtatcccatg agaagtcact ctccatagct tgtttttgta ggtttctcca
     4141 aatgtctgga tggtttctat acaagtccaa tgctgtttgg aaagtccaat ttaaccaata
     4201 aggagataga ttgtcaaagc taaagccagt accgcttcct tcgattggat tgaaagcgcg
     4261 aactgtatct cgcaagcctc caacttcatg gaccaatggc aaggttccat aacgcatagc
     4321 catcatttga gacaagccac acggttcaaa acgacttggc atgaggaaga ggtcacaagc
     4381 agcgtagatt tcctgagcaa gtttgacatc aaaagtgata tttgttgata gcttgtctgg
     4441 gtaaatctga gcaaaccatg agaaagctcc ttcaaaggct ggatcgccag ttcccaaaag
     4501 aacaatctga acatcttctt gcaagatatg gtgaagactt tcgaccacca catcaaaacc
     4561 tttttgacgt gtcaaacgag aaacaattcc caccagtgga acgtctgctc taacaggcaa
     4621 gccaactctt tcttgcaatt ttgccttatt tttggctttc ccagacaaat cttcctgatt
     4681 gaaatgatag tctaaaagag catccgtctg aggattataa agatcagcat caatcccatt
     4741 cacgatacca gatactttac cagactccat tttaagaatc tgatccaaat tacatccaaa
     4801 ctgactagtc ataatttcat gagcatagct aggtgaaacg gttgaaacac ggttcgcata
     4861 gagaatacct gccttcatcc agttcagaca gttgttccat cgaagggtgc catcagcgta
     4921 acgttcaaag ccaactccaa acaaatcacc caacattcct tctgaaaatt gtccttggaa
     4981 ttctaaatta tgaatggtta aaactgtttc aatgtcctca taggcttgaa tccaacggta
     5041 tttttccttc aacaagaaag gaatcatagc tgtatggtag tcatgaacat ggagaagatc
     5101 aggaataaag tcaatccttt ccatagcctc aatggcagcc agttggaaaa aggcaaagcg
     5161 ttctccgtca tcaaaatcac cgtaaacatg accacggaag aaataatatt gattgtcaat
     5221 aaagtagaag gttacaccat ttaatactgt tttcttaatt ccacaatact gtctgcgcca
     5281 accaacgctc acctcaaaat gaagcacatc ttcaatctga tttccaaatt tagcctctac
     5341 catatcatag tagggtaaaa tcactgcaac ttcgtgccca gcttttacca gtgattttgg
     5401 aagagcgcca atgacgtctc ccaagccacc tgtttttgaa aagggtgcac cctctgctgc
     5461 tacaaataaa attttcatga atgaatatcc tctgttactt tagcaccttt cttaaccaca
     5521 actggatgtt ctgcagttcc tcgaatcaca acaccaggct caacttcaac ccctttgtcc
     5581 aagatagcat attcgacctg agccccttct ccaataacaa cacgagggaa gagcaggcta
     5641 tctttaacca agctatccct atggacatga atattacgtg atagaacaga attagctact
     5701 tgaccttcaa taatactacc agaggcaaac tgagaagtgc ttaccttaga tgtattagca
     5761 tagtaagttg gctcttcgtt tttgaccttt gtataaatct tttggtttgg tgagaaaaga
     5821 gaatagaatt tttgtgattc aagcatatcg atattcgctt gataataaga tttaacagag
     5881 tgaatattgg ctagatagcc cgtgtactcg taggcgaaag ctccctcttt tacagccaaa
     5941 tcccgtaaaa catagcgcaa tttctctgga tgttcttttt tagcttcttc ttccaagtgt
     6001 tcaatcaacc aaggtgtatc aacgacaaag atatctgtag acatattgaa cgtttcagct
     6061 gttgacttgc tatcaaagag tttatgagaa agaacatggt ctgtttcatc tacatccaag
     6121 attgcattta cttctgaaat atctttctta gctagttttt tataaactac agtgataggc
     6181 tcttttgttg tactatgtag gtggaaaact tggttcaaat caatgttaat aagaacatcg
     6241 cagttgaggg caaccgtttg gtttgagcca gaacgtttca aataagtaag aagctgttgg
     6301 tagtattctt ttccaactgt actactttct acacgggtat tgtaaattcc tagatagtaa
     6361 tgactaagaa gggttgataa gccccactcg cgtcctgaac gaatatggtc aaatactgag
     6421 ctgatattat cctgctggaa aataccaaag acactacgaa cacctgcatt agcaaggctt
     6481 gaaagtggga agtcaatcaa acgatatttc ccaccaaatg gcaaacttgc tactggacgg
     6541 tggtccgtca atgtcgacat attgtgaaaa ccaactgtat ttcctaaaat ggcagaatat
     6601 ttatcaatct tcatctgttg ctacccccac tacttcatta tatcctacaa cttgtacttc
     6661 atctgttcca tcaatttcga caccgtcaga aataatcgca ccttcaccaa taatggcacg
     6721 tttaatctta gctccttgac caatgatagc tccactcatg ataactgaat caaggacttc
     6781 cgctccttcg cgaacttgcg cgcctgttga aaggatagaa tgtttaacag ttccatcaac
     6841 gaaacatccg tctacaacta atgagtcttc cacatgagca tttgccccga ggaagtttgg
     6901 tggtgaaatc aagtttcttg agtaaatctt ccattgacgg ttacgactat ccaaggcatt
     6961 ttctggagaa atatactcca tgttcgcttc ccaaagtgac tcaatagtac caacatcttt
     7021 ccaataacca ctaaattcgt aagcataaac actttcacct gactcaaggt aatttggaat
     7081 gacattttta ccaaagtctg acatgccaac cttgctcttt tcagcagcga ctaacatatt
     7141 acgaaggcgt tgccaatcaa aaatgtagat tcccatagaa gcttttgtag atttaggttg
     7201 agctggtttt tcttcaaatt caacaatacg attgttagca tctgtgttca tgataccaaa
     7261 acggcttgct tctttaagag ggacgtctaa aactgctact gtcaagctgg cattattatc
     7321 cttatgagac tggagcatat catcatagtc cattttgtag atgtgatccc cagacaaaat
     7381 caagacatac tcaggattga cactgtcgat atagtcgata ttttggtaaa tagcgtgact
     7441 agtcccctca aaccaacgat ttccttcact tgcagaataa ggttgaagaa tagagacacc
     7501 tgaattaata ccgtctagtc cccagcttga accattccca atatggttgt tgagagcaag
     7561 tggttgatac tgtgtaacga ccccaacatt atgaatccct gagttggcac agtttgatag
     7621 ggcaaagtca atgatacggt agcgcccacc aaattgcaca gctggttttg cgatgctttg
     7681 agtgagttta ccgagacgag ttccttgccc accagcaaga atcaaagcta acatttcatt
     7741 tttcattttc tactcctttt tggtttttat ttgtgacggt tttagtagat ttcaagcgac
     7801 gtttgatttt ccatacactt gctcccatag ccggtagagt aaaggttaag gtctgctcat
     7861 aatctttcca taggccttct tgcgtttgaa cagtttgatt atgttctttc caaacgcctc
     7921 cccactcttc caactcagta ttccatactt cttcgtaaat tcctgcaacg ggtagtccga
     7981 ttgtaaaatc tttccgctca acaggtacca tgttaaagat acagactaac atttctccct
     8041 ttttaccctt acgaataaag gaaagaacac tctggtctcg attatccgca tcaatgattt
     8101 caataccatc atagctggta tcgatttccc acagacagcg atgatctttg taaaactggt
     8161 ttagctgaga agtgaaatac ttcatcttag cattcattgg gtcttctagg ttagaccatt
     8221 ccaactgttc ttcagatttc cattctagga attgaccgta ttcgctaccc atgaagagca
     8281 atttcttacc agggtgacaa atttggtacg tatagagatt gcgcaagcct gcgaattgat
     8341 tgtaacgatc tccccacatc ttatgcatca tactcttctt gccatgaacc acttcatcgt
     8401 gcgagaatgg caagagataa ttctccttga aaacatacat aaagctgaaa gtcaccaggt
     8461 taaagtcata tttacgataa atcggatctt cttcgtagaa acggaggata tcattcatcc
     8521 agcccatgtt ccatttgtag tcaaatccta gaccaccaat ctctttcatt cccgtaatct
     8581 tggtcgcaga cgaactttct tctgcaatca tcatcacatc tggatattct aacttaataa
     8641 cttcattcaa gcgttgaaga aaataataac cttcatagtt gagatttccg ccatctttat
     8701 taggtgtcca tggagcatca tcatagtcca aatagagcat gttgctaaca gcatccacac
     8761 gaataccatc caaatgatag acatcaatcc aatgcttaat gcaagaaatt aagaaggact
     8821 gaacttcatt ttttccaagg tcaaaattaa gggcacccca accatggtta tgagccttat
     8881 tatggtcttg gtattcaaaa gtcggtgtcc catcataata ggctaaggca tcatcgttga
     8941 tggtaaagtg acctggtacc cagtccacaa taaccccaat attatgggta tgacactcct
     9001 cgacaaaatc ttgaaactcc tctggtcggc cataagcatg ctctaaagcg aagtaaccca
     9061 taagctgata cccccaactc aagcccaaag gatgggacat caagggcata aactcaatat
     9121 gagtatagtt catttcaacg agataaggaa tgagttcatc cttgagctgg gcaaaactat
     9181 aaggactgcc atcagaattt cttttccatg atccagcgtg aacttcataa atattgacag
     9241 gacgctcttc aaagccccaa cgttttcttc gtgccagcca aagtccatcc ttccatttct
     9301 tctcaggaag ctctgttaag attgcccctg ttcctggacg agcctcatac ctgacagcaa
     9361 aagggtcaat cttcatcagt tgatgaccat tttgacgtgt gacatgatat ttgtaaatat
     9421 gcccttcttg agccatattg gtaaagactt cccagacccc aaaatcattt cttaccattg
     9481 gaatctgatt ttcaatccag ttggtaaaat caccaaccaa gtgaacagcc tgagcattag
     9541 gtgcccaaac acggaaggta tagccatgct ctccatttag ttcttcccta tgtgctccta
     9601 gataatgttg gagataaaaa ttttcacccg tcataaaggt ttttaatgct tctctattat
     9661 ccatatactc cccttctcct gtaagcgttt tctatgtttt tattatacta ccttt
//
LOCUS       DAVGJL010000079         9660 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.079, whole genome shotgun sequence.
ACCESSION   DAVGJL010000079 DAVGJL010000000
VERSION     DAVGJL010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9660
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.079"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(55..1146)
                     /locus_tag="VVE42_001471"
     CDS             complement(55..1146)
                     /locus_tag="VVE42_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEU3073651.1"
                     /translation="MGKIVAIDLFSGAGGTTSGLKKSGIDVQVAVEIDSVAVKTYKLN
                     NPEVSVIDKDINVVSGDEVKEYLKIDTDDKLMLVACPPCQGFSTIGTNNENDERNQLV
                     FQFLRLINELNPDFLLMENVVGMTRTRNEKIFKKFLTSIDNNYSVIYDILNTADYGVP
                     QSRKRLVLHGIRKEILDNTNLTLNLPSKTHSNIPELDLLSWINADIIMDLPPIEGGEE
                     YIGEGIYNHVANKLSNLNIERITHIRNNGGSRTSLPKSLELNCHKNRLGHSDVYGILD
                     INKPSITITGGCMSYSKGRFGHPTQNRALSAREAARLQSFDDDYIFVGNRGQLAKQIG
                     NAVPVKLAQASGNYFMSVYREIANGQSDN"
     gene            complement(1227..3878)
                     /locus_tag="VVE42_001472"
     CDS             complement(1227..3878)
                     /locus_tag="VVE42_001472"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEU3073652.1"
                     /translation="MSKELSPKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ISRYDLGRESFLTKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VNLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGKNVILPIANKLIPIVGDEHADPEFGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAFEFSGMDRFEARKAVVAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNADGEMYVGEEAPEGDGWTQDEDVLDTWFSS
                     ALWPFSTMGWPEVDSEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVL
                     IHGLIRDEQGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNGGLTLDVAHDNVTKVATGEAGNVTDRWILHNLNETIAKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEDDKVITRSVLLYTLDKI
                     LRLLHSIMPFVTEEIFGQYAEGSIVTAAYPTVNPVFEDLAAHTGVESLKDLIRAVRNA
                     RAEVNVAPSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIYLPLADLLNVEEELARLDKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     EKDKQADYQAKYDATVARIDEMKKLVK"
     gene            complement(3871..4449)
                     /locus_tag="VVE42_001473"
     CDS             complement(3871..4449)
                     /locus_tag="VVE42_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU3073653.1"
                     /translation="MHLFIIGAPASGKMTIGQELSRLTDATLFYNHQAIDFALEIYQD
                     YTEEMWEFVRGITFSFLGASARNQRSVILTDVIDFSNQYHLIYLKQIQSLLDDYHQEI
                     LFVELETSLEECIRRNRTENRLKYKPLKRHIEVSEREIIETAETLQLNSQHQPNELNH
                     YFKINNTNLSAEEAAKQIQNKINKIEKGHTHV"
     gene            complement(4449..5009)
                     /locus_tag="VVE42_001474"
     CDS             complement(4449..5009)
                     /locus_tag="VVE42_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU3073654.1"
                     /translation="MARTELPDKIETERLVLRVRTVADTEDIHAYASLPEVAYPAGFL
                     PVKTLEDEIYYLGHILPNRNQKENFPAGYGIVVKGTDKVIGSVDFNHRHEDDVLEIDY
                     TLHPDYWGRGYVPEAARTLIDLAFKDLGLHKIELTCFGYNLQSKRVAEKLGFTLEARI
                     RDRKDAQGNCCDDLRYALLKSEWEVI"
     gene            complement(5094..5573)
                     /locus_tag="VVE42_001475"
     CDS             complement(5094..5573)
                     /locus_tag="VVE42_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEU3073655.1"
                     /translation="MKQSFKTNKLYYGFPVFILGYQDQNFSYNIMTCSSSYSLGDWVV
                     IGVVARENAAEQIKQYQKFTVNISDETSMLAMEQAGFISHQEKLERLGVHYEISERTQ
                     TPILDACPLVLDCRVDRIVEEDGICHIFAKILERLVAPELLDEKGHFKNQLFAQPIS"
     gene            complement(5856..6251)
                     /locus_tag="VVE42_001476"
     CDS             complement(5856..6251)
                     /locus_tag="VVE42_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEU3073656.1"
                     /translation="MSKKLNRKKQLRNGLRRAGAFSSTVTKVVDETKKVVKRAEQSAS
                     AAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANLGGLPLDRAKTFYDEGIKSASDFK
                     NWTEKELLALKGIGPATIKKLKENGIKFK"
     gene            complement(6347..6601)
                     /locus_tag="VVE42_001477"
     CDS             complement(6347..6601)
                     /locus_tag="VVE42_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEU3073657.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKAGKGFHDLPEGLFGERNY"
     gene            complement(6611..7945)
                     /locus_tag="VVE42_001478"
     CDS             complement(6611..7945)
                     /locus_tag="VVE42_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HEU3073658.1"
                     /translation="MADERIHVLRDILLELHNGASPESVQERFDATFAGVSAIEISLM
                     EHELMNSDSGVTFEDVMELCDVHANLFKNAIKGVEVSDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYEYMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTTAKSLPEVSISSVKEAFEAFATEFESMIFKEESIL
                     LMILLESFTQDDWLQIAEESDAYGYAIIRPSEKWVPERQSFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKDDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKTIMKGLREGTKDKY
                     EMWFKSESRGKFVHITYAAVHDEDGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            complement(7945..8178)
                     /locus_tag="VVE42_001479"
     CDS             complement(7945..8178)
                     /locus_tag="VVE42_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HEU3073659.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFKPLANPLMRNTVGR
                     KVSLKQGSKLAGTPMDKIVRTLEANGYEVIGLD"
     gene            8209..8283
                     /locus_tag="VVE42_001480"
                     /pseudo
     CDS             8209..8283
                     /locus_tag="VVE42_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            8272..8397
                     /locus_tag="VVE42_001481"
     CDS             8272..8397
                     /locus_tag="VVE42_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004238277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysyl-tRNA synthetase"
                     /protein_id="HEU3073660.1"
                     /translation="MDRTDEVSNHTYSKVKLTWFEEIFEEYKMILLLILYHMERE"
     gene            complement(8596..8778)
                     /locus_tag="VVE42_001482"
     CDS             complement(8596..8778)
                     /locus_tag="VVE42_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073661.1"
                     /translation="MGLIKTLAKIYGNYFLTVQGVKVMKTIKKDDHVVVGLGKLFIAD
                     KLMDTARWLIKPEDKK"
     gene            complement(8780..9559)
                     /locus_tag="VVE42_001483"
     CDS             complement(8780..9559)
                     /locus_tag="VVE42_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073662.1"
                     /translation="MAEQDLAMQVLQQVVKLPVVKVDRSKFLVDKFSKELGPQDIPTL
                     LEQGPTSLLSQEILDRVANACIRDNVLLASGTSVLAGLPGGLAMAITIPADVAQFYGF
                     SLKLAQELGYIYGYEDLWASRDELSEDAQNTLLLYLGVMLGVNGTAALLRAGGITIAK
                     QVMKTVPNKALTKTLWYPILKKVLRIFGVNLTKGGLAKGMGKFIPILGGIISGGLTFA
                     TMKPMGESLQKELSKLVNYSEVQYQEDVETIRKEAEIIEGE"
BASE COUNT         2746 a         2168 c         1803 g         2943 t
ORIGIN      
        1 ggataaaatt gatttttatt tttaaaaaaa tttttagtta ttccttctaa actctcaatt
       61 atctgattgt ccatttgcta tctccctata aactgacata aaataatttc cactagcttg
      121 agctaatttt actggaactg cattaccaat ttgcttagct aattgacctc tatttccaac
      181 aaaaatatag tcatcatcaa aagactgtaa tcttgcagct tctcttgcac ttaaagctct
      241 attttgagtt gggtgcccaa atctaccttt tgaatatgac atacaccctc ctgtaatagt
      301 aatggaaggt ttgttgatat ctaatattcc atatacgtca ctatgaccta aacgattttt
      361 atgacaattt aattctaaag attttggcaa agaggttcta cttcccccat tatttctaat
      421 atgagtaatt ctctcaatat ttaaattaga tagtttgttt gcaacatgat tgtagatacc
      481 ttctccaata tattcttcac caccttcaat aggaggcaag tccattatta tatccgcatt
      541 aatccatgaa agaaggtcca attctggtat atttgaatga gttttggaag gcaaattcaa
      601 agttaaattc gtgttatcaa gaatttcttt tctaatcccg tgtaacacca atctttttct
      661 agattgagga actccataat cagctgtatt taaaatgtca taaataacac tataattatt
      721 atcgattgat gtcaaaaatt ttttaaagat tttttcattt cttgttctag tcatacccac
      781 aacattttcc attaatagaa aatcaggatt caattcatta atcaaacgaa gaaattgaaa
      841 aacaagttga ttcctctcgt cattttcatt attagtacca atagttgaaa aaccttgaca
      901 cggtgggcat gccacgagca ttagtttatc gtctgtatca attttaagat actcttttac
      961 ttcatctcct gatactacat ttatatcttt atctattaca gacacctcag gattatttaa
     1021 cttataagtt ttaacagcta cggaatcaat ttcaacagca acttgaacat ctatgcctga
     1081 ttttttaagg ccacttgtag tgccacctgc accgctaaat aaatcaatag caacaatttt
     1141 ccccatagta ttctcctaaa gtttctcctt tttattataa cattatcaaa tgtaaaaccc
     1201 aacccgatag ggttaggttt ttaacatcat ttcaccaact tcttcatctc atcaatacgt
     1261 gctacggtcg catcatactt ggcttggtag tcggcttgtt tgtctttttc tttttggacg
     1321 acttctggtt tggcgttggc tacgaagcgt tcgttagaga gtttcttacc aaccatgtcc
     1381 agttcttttt gccatttagc aagttccttg tcgagacggg ccagttcttc ttcaacattg
     1441 aggagatcgg ccagtggcag gtagatttct gctcctgtga tgacacttga catagccagt
     1501 tcaggtgcag ggatggttga tgcgatttcc aaatgttctg gatttgtaaa gcgtttgatg
     1561 tagttgacat tgctgttaaa gaaggcttcc aagtcactat cgctagtctt aacaaggatg
     1621 gtgataggtt tgcttggtgc tacatttact tccgcacgcg cattacgaac ggcacggatc
     1681 aagtctttga gactttccac accagtgtgg gcagcaaggt cctcaaagac tgggttgaca
     1741 gttgggtatg ctgctgtcac gatagagcct tcagcgtact gaccaaagat ttcttctgtc
     1801 acgaacggca ttattgagtg aaggagacga aggattttat ccagcgtata gaggagaaca
     1861 gatcgagtaa tgaccttatc gtcttcattg tcgctgtaaa gaacttcctt ggtcaactca
     1921 acataccagt tggcaaattc ttcccaaatg aagttgtaaa ggatatgacc agccacacca
     1981 aactcgaact tatcaaagtt ttcagtaact tttgcaatgg tttcgttgag attgtggaga
     2041 atccagcggt ccgtcacatt accagcctca cctgttgcaa cttttgtgac attgtcatgc
     2101 gccacatcca gcgtcaaacc tccattgttc atgaggatat agcgagaaat gttccaaatc
     2161 ttgttaataa agttccatga agcatccatt ttctcataag agaaacgcac gtcttgtcct
     2221 ggcgcagaac cgtttgaaag gaaccatcga agggcatcgg caccgtattt ctcgataaca
     2281 tccattgggt caatcccgtt accgagagac ttagacatct tgcgtccttg ctcgtcacga
     2341 ataagaccgt ggataaggac gttttggaat ggttgacggc ctgtgaattc caatgactgg
     2401 aagatcatac gagacaccca gaagaagatg atgtcgtaac ctgttaccaa ggttgaagtt
     2461 gggaaataac gtttaaagtc ttctgagtcg acttcaggcc agcccatggt tgaaaatggc
     2521 cagagggcag aactgaacca agtatccaag acgtcttcgt cctgagtcca tccgtcacct
     2581 tctggagctt cttcgccgac atacatttca ccatcagcat tgtaccaggc agggatttgg
     2641 tgaccccacc aaagctgacg agagataacc cagtcgtgga cattttccat ccattgaagg
     2701 aaggtatcgt tgaaacgagg tgggtagaat tcgaccttgt cctctgtgtc ttggttggca
     2761 atggcgttct tagccaattg gtccatcttg acgaaccatt gagtagacaa gcgtggctca
     2821 actacgacac ctgtacgttc tgagtgacca acactgtgga cacgtttttc gattttgaca
     2881 agggcaccga tttcttccaa cttagcaacg actgccttac gagcttcaaa acgatccatg
     2941 cctgaaaatt caaaggcaag ctcattcata gttccgtcgt cgttcatgac gttgacttgt
     3001 ggcaagttat gacgttggcc aaccaagaag tcatttggat cgtgggcagg tgtgattttc
     3061 acgacaccag taccaaactc aggatctgcg tgctcatctc caacgattgg gatgagttta
     3121 ttagcgattg gaaggatgac gtttttacca atcaagtcct tgtagcgcgg gtcttctgga
     3181 ttaaccgcaa ccgcaacgtc cccaaacata gtctcaggac gagttgtagc aacttcaagg
     3241 gcgcgtgaac catcttccag catgtaattc atgtggtaga aggcaccttc cacatccttg
     3301 tgaatcacct caatatcaga aagggctgtg cgagctgctg ggtcccagtt gatgataaac
     3361 tcaccacggt agatccagcc tttcttgtaa aggttcacaa agaccttacg aacagctttt
     3421 gacaaacctt cgtcaagagt gaaacgctca cgagaatagt ctacagagag ccccatcttg
     3481 ccccattgtt ccttgatggt agtggcatat tcgtctttcc attcccagac tttcgtcaag
     3541 aaagactcac gaccaaggtc atagcgggaa atgccctcac cacgcaagcg ctcctcaacc
     3601 ttagcctgag tggcaatccc tgcgtggtcc atcccaggaa gccaaagggt atcaaaacct
     3661 tgcatgcgtt tttgacggat gataatatct tgcaaagttg tatcccaagc gtgaccaagg
     3721 tggagtttcc cagttacgtt tggtggtgga atcacgattg aataaggctt agccttttga
     3781 tcgcctgaag gcttgaaaac atcggcatca agccattttt ggtaacgacc agcctcaacc
     3841 tcggctggat tgtatttagg tgaaagttct ttagacatgt gtgtgtcctt tctctatttt
     3901 gtttatttta ttttgaatct gcttagcagc ttcttctgca gacaaattcg tattatttat
     3961 tttaaagtag tggttcaact cattcggttg atgttgggaa tttaattgaa gtgtttcagc
     4021 ggtctctata atttctcttt cagatacctc aatatgtcgt tttaagggtt tgtactttaa
     4081 tcgattctcc gttcgatttc gacgtatgca ctcttcaaga cttgtttcca actcaacaaa
     4141 aagaatctct tgatgataat catccaacaa actctgaatt tgctttaaat atatcagatg
     4201 atactgattt gaaaaatcaa ttacatctgt taaaattact gatcgctgat ttcttgcact
     4261 cgctccaagg aatgaaaaag tgattccacg aacaaattcc cacatttcct ctgtatagtc
     4321 ctgatagatt tctagtgcaa aatcgatggc ttgatggtta taaaataggg tagcatccgt
     4381 cagtcgagat aattcttgac caatggtcat ttttcctgat gctggagcac caatgatgaa
     4441 aagatgcatc aaatcacctc ccattcactc ttcagcaagg catatcttaa atcatcacag
     4501 cagttgcctt gagcatcttt gcggtctctt atgcgagctt cgagggtaaa gccaagcttt
     4561 tccgcgactc gtttactttg aaggttatat ccaaagcaag ttagttcaat cttgtgaaga
     4621 cccaaatctt taaaggctaa gtcaatcaag gtacgcgcag cttctggcac ataacctcgc
     4681 ccccaatagt ctgggtgtaa ggtatagtcg atttccagca catcatcttc atggcgatgg
     4741 ttgaaatcaa cagaaccaat gactttatcg gttcctttga cgacaattcc gtagccagct
     4801 gggaaatttt ccttttgatt gcgattggga aggatatgcc ccagatagta aatctcatct
     4861 tccaaggtct tgacgggcag aaaacctgct gggtaggcga cctctggcaa actagcatag
     4921 gcatggatat cctcagtatc cgccactgtg cggactcgta aaacgagacg ttctgtttcg
     4981 attttatctg gcagctcagt tcttgccatc cttcttcctc gcttttttga tgaaactacc
     5041 cttcatatct acacgcttgt ccagatagca ataaacgcgc tgatatccat ctctcatgaa
     5101 ataggttggg caaacagttg atttttaaaa tgtccctttt catccaggag ttctggggca
     5161 acaagtcgct caagaatctt ggcaaagatg tggcaaatac cgtcttcctc aacaatccta
     5221 tctacccgac aatctaaaac aagtggacag gcgtctaaaa taggagtctg agttcgttca
     5281 gaaatttcat aatgcactcc caaacgttcc aatttctcct gatgactgat aaaaccagcc
     5341 tgctccatcg caagcataga agtttcatca gaaatattca cagtaaattt ttgatattgt
     5401 ttgatctgct ctgcggcatt ctctctcgca acgactccaa tcacaaccca atctcctaga
     5461 ctataagagg aactacaggt catgatgtta tagctaaaat tctgatcttg atatcctaaa
     5521 ataaaaacag gaaaaccata atatagttta ttagttttaa atgattgttt tataacaacc
     5581 tcctttgact aagacacaaa agaaaagccc tgccatctac atgacaggga cgaatgtgtt
     5641 tatccgcggt accacccaat ttcgggccaa gcccgcatct ttagtcttta aaataaaaag
     5701 acagaaatat ttcatttttt cagtcattag caaccagttt tgacacattt gtggacttct
     5761 cagcgccgcc actttctgta aaatgtggga ttcaaaacac ctctaatggc tctattgtag
     5821 caagtttttc tcggaaatgc aaggctcaag aaaaattact tgaacttgat gccattttct
     5881 ttcaatttct tgatggtagc tgggccgatt cctttcaagg caaggagttc tttttcagtc
     5941 cagtttttga aatctgaagc agacttgatt ccttcatcat agaaagtctt ggcacgatca
     6001 agtggaagtc cacccaagtt tgctgcaaaa tcttctacag aattagctac tttttgagct
     6061 tgctctttgg tagcttctac tgcttgttca acttttttag aaacagcctt accagctgcg
     6121 cttgctgact gttctgcacg cttcacgact ttttttgtct catctacaac cttagtcaca
     6181 gtacttgaaa aggcacctgc gcgacggagg ccatttcgta attgtttttt acgattgagt
     6241 ttctttgaca tgataaaatc ctttcaataa aaaatccctg ctctgacaag gatttaacat
     6301 aaacattcta tcaagatttt agtgaaaaat caagaatctt tctcgtttaa taatttcgct
     6361 caccaaacaa gccttctggc aaatcatgga atcccttgcc tgctttgaag ttttcaaact
     6421 taccaagcaa gaactgataa gcatccaagc gactctcgta gcgaatcatg gcatctttgg
     6481 cacgctcgtc ctggtcttct tcgtaaactt tttgactttc cttgtgcgcc atgtcgttaa
     6541 tcatgatttg ggtattgacc caagcttcaa attccttcat aaattcttgt tcgtaactca
     6601 ttttttctcc ttattctaat ccacgaaaat agtccgtatc aatctcacgg tagggctgga
     6661 tatcctgaac atactccaac actccttgga attctccgtc ttcatcgtgt actgcagcat
     6721 aggtgatgtg gacaaactta cctcgcgact cagacttgaa ccacatttca tacttgtcct
     6781 tggtcccctc acgaagcccc ttcatgatag ttttgacctt gtccaagtac ttaggcggat
     6841 ggcagagttc gacattgcgc ccgacttgag acggcgtccg tttgaaaatc atctcatcag
     6901 ctggcgtatt gtcattgtaa tactggaaaa tatcatcttt attgacaaag gtaatctcca
     6961 tagggaggtg attgaggatg agattggcct gctcgactga aagatagcca ttaccaaaag
     7021 cctgttgact atggcggtcc agcacagctt ccttttcctt aggggtaaag gtaatggtaa
     7081 aatggccttc tggcgtatct atgacttgtt gaacttgacc ttctgccgta tctagctgta
     7141 caggctcctc tgcaatcttt tcctcaataa agctctgtcg ttctggcacc catttctctg
     7201 acggacggat gatggcataa ccataggcat cgctctcctc cgcaatctga agccagtcat
     7261 cctgagtaaa agactcaagg agaatcatga ggaggatgga ctcttccttg aaaatcatac
     7321 tttcaaactc tgtcgcaaaa gcttcaaaag cttcctttac actgctaatt gacacttctg
     7381 gtagtgactt ggctgtcgtt agagctgttt gaaagagttc cctaatctga tcatccactc
     7441 cccacataac tttgggaggt gaatcgtgtc catagcgctc catgatagga aagaagagtt
     7501 cttccttacg ttggtaatgg atgtcaaatt gacccacaag tcccatctga cgcaccaaac
     7561 ccttacgcat ctccgccagc atttcctcgt cttccatata ctcataggta tctaacaatc
     7621 tacgaatgcg aatcaaggcc gcacggagag ccagattttc ttctttgaag acacgaactg
     7681 ggtgacctgg atgctcagta tctgaaactt cgacaccttt gatagcattt ttaaaaagat
     7741 tggcatggac atcacagagt tccataacat cttcaaaggt gacacctgag tctgagttca
     7801 tcagctcgtg ctccataagg gaaatctcga tggctgagac acccgcaaag gtcgcatcaa
     7861 agcgctcttg aaccgactca ggagaggcgc cattgtgcaa ttctaacaaa atatcccgta
     7921 ggacatgaat ccgttcatct gccattagtc taatccaatc acttcgtagc cattcgcttc
     7981 cagtgtgcgt acaatcttgt ccataggagt tcctgctagc ttagaaccct gtttaagtga
     8041 tactttacga ccaactgtat tgcgcattaa gggattggca aggggtttaa aacccaactc
     8101 cactagaatt tccaagactt ctggatgctt gtccaccact tctgcaacag gaattgacac
     8161 atcgatgata ttgtccatga cgacctccat tgtatgttct aatactcaat gaaaatcaaa
     8221 gagcaaacta ggaagctagc cgcaggttgc tcaaaacagt gctttgaggt tgtggataga
     8281 actgacgaag tcagtaacca tacctacagc aaggtgaagc tgacgtggtt tgaagagatt
     8341 ttcgaagagt ataaaatgat tttactgctt atattatacc atatggaaag agaataaaaa
     8401 actagcagtg gagttcctgc tagttctctg cgacttggat taccatctag ataagtttaa
     8461 agagccaatc caatatctta aacactatct tgtaaaacag caattggact gcaaaagaga
     8521 tgatcatcca aaagaatttc acaatcccaa taatcggttt gataaaaata atggcaagaa
     8581 gtccccagaa aaatttcatt ttttatcctc tggcttaatg agccaccgag ccgtatccat
     8641 tagcttatct gcaataaaga gttttcccag accaacaacg acatggtcat ctttctttat
     8701 cgttttcatt acttttacac cttgcacggt caaaaagtaa ttaccataaa ttttagctag
     8761 agtttttatg agtcccatat tactctcctt cgatgatttc agcctctttt cggattgttt
     8821 caacatcttc ttgatattga acttcactat agttaactag cttggacaat tctttctgca
     8881 agctttcacc catcggtttc atagttgcaa aggttaaacc acctgaaatg ataccaccca
     8941 agataggaat aaatttcccc attcctttgg ccagccctcc cttggtaaga ttcacaccaa
     9001 atattcttaa gactttcttc aaaataggat accaaagcgt ctttgttaaa gctttattag
     9061 gtactgtttt cattacctgt ttggcaattg ttataccacc agcacgtagc aaagcagcag
     9121 ttccattcac ccccaacatg acgcctagat aaagcaaaag ggtattttga gcatcttcac
     9181 tcaactcatc gcgtgaagcc caaagatcct cataaccata aatataacct aattcttggg
     9241 ccaatttcag agagaaacca taaaattgag ccacatcagc tggaatggta attgccatag
     9301 caagccctcc aggtaatcct gccaaaacag aagtcccact cgctaataaa acattatccc
     9361 taatacaggc attagccact cgatctaaga tttcttgaga taagagagac gttggacctt
     9421 gttccaataa ggtaggaatg tcctgtggac ccaattcttt ggaaaactta tccactaaaa
     9481 atttcgaacg atcaacctta acaactggta gttttaccac ttgttgtaat acttgcatag
     9541 ctaagtcttg ttcagccata tacaaactcc ttatttcttt tttacacctt atcatattat
     9601 atttcctaca aaaaaagcat tttctaatta aataacatta aaaaatacag aatccctttt
//
LOCUS       DAVGJL010000080         9633 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.080, whole genome shotgun sequence.
ACCESSION   DAVGJL010000080 DAVGJL010000000
VERSION     DAVGJL010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9633
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.080"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(<1..795)
                     /locus_tag="VVE42_001484"
     CDS             complement(<1..795)
                     /locus_tag="VVE42_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEU3073663.1"
                     /translation="MQEHYTPKGKHLTIDNRRLIERWKNENKSNREIAGLLGKAPQTI
                     HNEVKRGTTLQQVRKGLYKKVYSADYAQTVYQFNRKRSVKKLILTKEIREKILHYHKQ
                     KFSPEMMVNKKQVKVGISTIYYWFHNGHYEIDTVLLTKIKNYCLLVLTDRRSRHQIIR
                     LIPNKTAESVNQALTLLLGEHRILSITADNGSEFKRLSEVFPEEHIYYAHAYSSWERG
                     SNENHNRLIRRWLPKGTKKTTPKEVAFIENWINNYPKKCLDYKSPSE"
     gene            1226..1525
                     /locus_tag="VVE42_001485"
     CDS             1226..1525
                     /locus_tag="VVE42_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEU3073664.1"
                     /translation="MKTKKHRLLALVLISSFTLLGAASAAVQYPDGGVWTYGEGSGGG
                     WAFSNYYHGKKYHYSSIVSRWDGHSDKGEAPAGKTSYAWIWTKWGEQVAFYCDYD"
     gene            1596..3617
                     /locus_tag="VVE42_001486"
     CDS             1596..3617
                     /locus_tag="VVE42_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU3073665.1"
                     /translation="MKKLFILLSTFFLSFFLAWIIVLRAPQYLYASYDSVSLLRVKKD
                     TQEPTREVFEQELENFANSEQSLIVRRIVEPSKDGTTHFTYATYGQGTLPKEFQEASQ
                     ESRERSDPLNSYLLLSGSLTKEKLADKLGDLGYKASADRKIPPYFLAFRILLNPLILI
                     SLAIFGLSFFALVIITRIKEMRAAGIKLFSGQTLLSIMGDSLSTDIKWLLLSALLSFL
                     GGGVVLFSQGLFYPILLATYGFGISFYLLFLLAISILLMLLYLMSLSYKALVPVIKGR
                     LPLKRLMILTLLCQLVAVFTVGYAVKTGLTSYQRLKELEISKQAWQDRADYYQISFGL
                     GDRGKDTENQSKWYAFAKEAIEEEQALYVKDNLLHFANPQGKNEQGETLDTYSPDANT
                     LYVSPSYLDKEKVVVDAETKQKLAHLQKGEFILLLPEHLRSREAELKKVFEERLSYYG
                     KSGEEASAPLDYEMKAHVSYLSMGEKRFVYNNGENPVSTQYLTDPILVVFTPTSTGDS
                     FISLSSWSINAGKQLFIKGYESGLELLKKAGIYEQVSYLKEGRSVYLTRYNEVQTETA
                     TLILGAIVGIASSLLLFYSVNLLYFEQFRRDILIKRISGLRFFETHAQYMVSQFASFV
                     FGASLFILSSRDLVIGLLTLLVFLASAVLTLYRQAQKESRVSMTIMKGK"
     gene            3619..4260
                     /locus_tag="VVE42_001487"
     CDS             3619..4260
                     /locus_tag="VVE42_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3073666.1"
                     /translation="MIELKNISKKFGSRQLFSDMNLHFEGGKIYALIGTSGCGKTTLL
                     NMIGRLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIESQTVKENLNLGLV
                     GKKLKEKEKISLMKQALNRVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTASLDPKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            4364..5716
                     /locus_tag="VVE42_001488"
     CDS             4364..5716
                     /locus_tag="VVE42_001488"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEU3073667.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQSLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYNNPMIEKALAKGIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQTASD
                     KDTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGSFSEYVAAKWNIQNKMTAAD
                     FLVLNFNQDLAKDLTSKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPG
                     SHNVENALATIAVAKLRDVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            5720..6778
                     /locus_tag="VVE42_001489"
     CDS             5720..6778
                     /locus_tag="VVE42_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEU3073668.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKRGIEHQ
                     EILKSGLDVTFHSIATGKLRRYFSWQNMLDVFKVGWGIVQSLFIMLRLRPQTLFSKGG
                     FVSVPPVIAARVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLAKVEHVGA
                     VTKVSDKNTPEPDELVDIQTHFNPKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELRQNLFRVDYVTDLYQPLMELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQIENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            6788..7996
                     /gene="divIB"
                     /locus_tag="VVE42_001490"
     CDS             6788..7996
                     /gene="divIB"
                     /locus_tag="VVE42_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HEU3073669.1"
                     /translation="MSKDKKNEDKETLEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     EKERQARMGEESEKSEDKQDQESETDQEDSESAKEESEEKVASSEADKEKEEKEEKEE
                     PESKEKEEQDKKLAKKATKEKPAKAKIPGIHILRAFTILFPSLLLLFVSAYLLSPYAT
                     MKDIRVEGTVQTTADDIRQASGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQF
                     PTKFTIKVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYLSVLFNDSEQIK
                     VFVSELAQISPELKAAIQKVELAPSKVTSDLIRLTMNDSDEVLVPLSEMSKKLPYYSK
                     IKPQLSEPSVVDMEAGIYSYTVADKLIMEAEEKAKQEAKEAEKKQEEEQKKQEEESNR
                     NQTTQRSSRR"
     gene            8229..8930
                     /gene="pyrF"
                     /locus_tag="VVE42_001491"
     CDS             8229..8930
                     /gene="pyrF"
                     /locus_tag="VVE42_001491"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEU3073670.1"
                     /translation="MREHRPIIALDFPSFEAVKEFLALFSAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKVLSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
                     QVIKQATNPDFICLTPGIRPAGVAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPV
                     AAYHAIKDEWTQDWN"
     gene            8964..9596
                     /locus_tag="VVE42_001492"
     CDS             8964..9596
                     /locus_tag="VVE42_001492"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEU3073671.1"
                     /translation="MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVEAIKEAFPEVEVIAGTATAGIPHGAIIADKMDLPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFADAGVKLVTLSNYSELIHLAQEEGYITPEGLDLLKRFKEDQENWQEG"
BASE COUNT         2923 a         1788 c         2111 g         2811 t
ORIGIN      
        1 ttcacttggc gacttgtagt ccaagcattt tttagggtag ttgttaatcc aattttcgat
       61 aaaagctact tctttcggag tcgttttctt ggttccttta ggtaaccatc tccgaattaa
      121 tcgattatga ttttcatttg aacctctctc ccatgaagag taagcatgtg cgtagtagat
      181 atgttcctca ggaaatacct cagacaatcg tttgaactcc gaaccattat ctgcagtaat
      241 ggacagaata cgatgctccc ctaatagtaa cgtaagcgcc tgattgacag attcagcagt
      301 tttatttgga attaacctta taatttggtg tctgctccgc cggtcggtta agactaacag
      361 gcaataattt tttatcttag tcagtaggac ggtatcaatt tcataatgac cattatgaaa
      421 ccagtagtag atggttgaaa taccaacttt cacttgcttc ttgttaacca tcatttcagg
      481 cgaaaatttt tgcttatgat agtgtaagat cttctctctg atttcctttg ttaaaattaa
      541 ctttttcacc gaccgttttc gattgaattg gtaaacagtt tgtgcgtaat cggcagaata
      601 gacctttttg tatagccctt ttctcacttg ttgtaaagtt gtacctcttt tgacttcatt
      661 atgaatcgtt tgaggcgcct ttcctaacaa gcctgcaatt tcacgattgg acttattttc
      721 attcttccac cgctcaatca agcgacggtt atctattgtc aaatgtttcc cttttggggt
      781 ataatgttct tgcatctctg tgcctttcct tgtgtttgtg gtgaacaaca agtatagcac
      841 agaggtgttt tcttatacct taaatcacat ttcatttgac aacaggaacc cttgaacctg
      901 ttttttcagc taaaccaagg ctatgattag ccttggccac ctagcctaac agtttcaaac
      961 ctgttatcaa atgaaatcat caacttactc aaacatagtg aaacatctaa ttcttgacta
     1021 aggtaaggtg gctaacttca ttatagaact ttcattcata gaggtaatat agtaaaatat
     1081 ctaaaaaatt cgattttttc cttgacaaca aataataaaa atgttattct catcacataa
     1141 gaaagcggtt tcaacaaaag aaagaggagg gtttattttg ttgcattgtt gctcaaggtg
     1201 tgcttttttt gaaagaaaaa ataaaatgaa aacgaaaaaa catagattac ttgctctagt
     1261 tcttatttca agttttacat tattgggagc tgcatcagct gctgtacaat atccagatgg
     1321 aggagtatgg acatatggag aaggttcagg aggtggttgg gctttttcaa attactatca
     1381 tggtaaaaaa tatcattatt catccatcgt gagcagatgg gatggtcatt ctgataaagg
     1441 agaagctcct gctggaaaaa cctcctatgc atggatttgg actaaatggg gagaacaagt
     1501 agcattttac tgtgattatg actaatcgtt cattgattta aatcgttccc cttgtaagaa
     1561 attacagggg gaattttgtt tacaaggagt ctactatgaa gaaactattc atattattat
     1621 caactttttt tctcagcttc ttccttgctt ggattattgt cttacgtgcg ccacaatatt
     1681 tatatgcaag ctatgattcc gtttctttac ttcgtgtcaa aaaagatact caggaaccga
     1741 cgcgtgaggt atttgaacag gaattggaga attttgcaaa ctcagaacag agtttaatag
     1801 ttagaagaat tgtagagccg agtaaggatg ggacgactca ctttacttat gcaacttatg
     1861 gtcagggaac tttaccaaaa gaattccaag aagctagcca agaaagtcgt gaacgtagtg
     1921 atccgctaaa tagttatctc cttttgtcag gctccttgac gaaagaaaag cttgccgata
     1981 aattaggaga tttgggttat aaagcaagtg ctgaccgaaa gataccgccc tattttcttg
     2041 cttttcgaat attactaaat ccccttattt taattagttt agcaatattt ggcttatctt
     2101 tctttgcttt agtgattatc actcggatta aggaaatgag agcagcaggt ataaaactct
     2161 tttctggtca gactctctta tccatcatgg gggattcttt atctactgat atcaagtggc
     2221 tccttctatc agccctcctt tccttcctag gtgggggagt cgttcttttt agtcaaggtt
     2281 tgttttatcc tatcttgtta gccacctatg gttttgggat tagtttctat ctgttgtttt
     2341 tattggcgat ttcaatttta ctaatgcttc tttatctaat gagtttgagt tacaaagcat
     2401 tagttcccgt tattaagggg agattacccc ttaaacgcct gatgatttta accctattgt
     2461 gtcagttggt tgctgtcttt acagtaggat acgctgttaa gacaggtttg acgtcttacc
     2521 aacgattgaa agaacttgaa atttcaaaac aagcatggca ggatagagca gactattatc
     2581 aaatttcttt tggcttaggt gatagaggaa aagatacaga aaatcagagc aagtggtatg
     2641 cctttgccaa ggaagcaatc gaagaagaac aagctcttta tgtaaaggat aatctgctcc
     2701 attttgccaa tccacaagga aaaaatgaac agggagagac actggatacc tatagtccag
     2761 atgctaatac gctctatgtt agtcccagtt atttggacaa ggaaaaggtc gtggtagatg
     2821 ctgagaccaa acagaagtta gcccatctcc aaaaaggtga gtttatcctc ttgctcccag
     2881 aacatttgcg ctctcgagaa gcagaactta agaaagtttt tgaagaaaga ttgagttatt
     2941 atggaaaatc tggtgaggag gcaagtgctc ctttggatta tgagatgaaa gcgcacgtta
     3001 gttatctttc aatgggagaa aagcggtttg tttataataa cggtgagaat cccgtatcca
     3061 ctcagtattt aactgatccg attttagtgg tattcacgcc gacttctaca ggtgatagtt
     3121 ttatatcctt atctagttgg tctatcaatg ctggaaaaca actctttatc aaaggatatg
     3181 agagtgggct agaactcttg aagaaagctg gaatttatga gcaagtatcc tatcttaaag
     3241 aaggaagaag tgtttatcta actcgttata atgaagttca aactgaaaca gcaactttaa
     3301 tcttaggagc tattgttgga atagccagtt ccttgttact cttttattct gtcaatcttc
     3361 tatatttcga gcaattccgc cgagatatct tgattaaacg aatttcaggt ttacgatttt
     3421 ttgaaacaca tgctcagtat atggttagcc aatttgccag ttttgtattt ggtgctagtc
     3481 tctttatttt aagcagtcga gacttggtga ttggcttgct cactttattg gtctttctag
     3541 ctagtgcagt tttgacgctt taccgtcaag cgcagaaaga atctcgtgtt tctatgacaa
     3601 ttatgaaagg aaaataggat gattgaacta aagaatatat ctaaaaaatt tggaagccgt
     3661 cagctatttt cagatatgaa tcttcatttt gaaggtggga aaatttatgc cttaatcggt
     3721 acaagtggct gtggtaagac aacactcttg aatatgattg gacgattaga gccatatgac
     3781 aaagggcaaa tcatatatga tggcacttct cttaaggaca ttaagccttc tgttttcttt
     3841 agagattact taggatactt atttcaagat tttggcttaa ttgaaagcca aaccgtcaaa
     3901 gagaatctca atctgggttt agttggtaaa aagttgaagg aaaaagagaa aatctctttg
     3961 atgaaacaag ctctaaaccg tgtaaacctc tcttatttgg atttgaagca acctatattt
     4021 gagttatcag gaggagaagc acaacgtgtt gcactagcga agataatttt aaaggatcca
     4081 cctttgatcc tcgcagatga accaaccgct tccttagacc ccaaaaactc tgaggaatta
     4141 ctttccatcc tagaatcttt aaaaaatccg aatcggacca ttattattgc gacccataat
     4201 cctctgattt gggagcaagt ggatcaggtc attcgagtta ccgatttatc acatagataa
     4261 tatggtaaga ttcagttaga agaaagagtc acaaacacac tttgtggctt ttttatttcc
     4321 ataaaaatgg taaaatagta ggagtagaaa tggagttcga gacatgaaag taatagatca
     4381 atttaaaaat aagaaagttc ttgttttagg tttggccaag tctggtgaat ctgcagctcg
     4441 tttgttggat aagctaggcg ccatagtgac agtaaatgac gggaaaccat tcgaggacaa
     4501 tccagctgcg caaagtttgc tggaagaagg gatcaaggtc attacaggtg gccatccttt
     4561 ggaactcttg gatgaagagt ttgcccttat ggtgaaaaat ccaggtatcc cctacaacaa
     4621 tcccatgatt gaaaaggctt tggccaaggg aattccagtc ttgactgagg tggaattggc
     4681 ttatttgatt tcagaagcac cgattattgg tatcacagga tcgaacggta agacaaccac
     4741 aacgactatg attggggaag ttttgactgc tgctgggcaa catggtcttt tatcagggaa
     4801 tatcggctat ccagctagtc aagtggccca aactgcgtcg gacaaggaca cgcttgttat
     4861 ggaactttct tctttccaac tcatgggtgt tcaagaattt catccagaga ttgcggttat
     4921 taccaacctc atgccaactc atatcgacta ccatgggtca ttttcggaat atgtagcagc
     4981 caagtggaat atccagaaca agatgacagc agctgatttc cttgtcttga actttaatca
     5041 agacttggca aaagacttga cttccaagac agaagccact gttgtaccat tttcaacact
     5101 tgaaaaggtt gatggagctt atctggaaga tggtcaactc tacttccgtg gtgaagtagt
     5161 catggcagcg aatgaaatcg gtgttccagg tagccacaat gtggaaaatg cccttgcgac
     5221 tattgctgta gccaagcttc gtgatgtgga caatcaaacc atcaaggaaa ctctttcagc
     5281 cttcggtggt gtcaaacacc gtctccagtt tgtggatgac atcaagggtg ttaaattcta
     5341 taacgacagt aaatcaacta atatcttggc tactcaaaaa gccttatcag gatttgacaa
     5401 cagcaaggtc gtcttgattg caggtggttt ggaccgtggc aatgagtttg acgaattggt
     5461 gccagacatt actggactca agaagatggt catcctgggt caatctgcag aacgtgtcaa
     5521 acgggcagca gacaaggctg gtgtcgctta tgtggaggcg acagatattg cagatgcgac
     5581 ccgcaaggcc tatgaacttg cgactcaagg agatgtggtt cttcttagtc ctgccaatgc
     5641 cagctgggac atgtatgcta actttgaagt acgtggcgac ctctttatcg acacagtagc
     5701 ggagttaaaa gaataaaata tgaaaaaaat tgtctttaca ggtgggggga cggttggaca
     5761 cgttaccctc aatcttttgt taatgcccaa gttcatcgaa gatggttggg aagtccacta
     5821 tatcggggac aagcgtggta tcgaacacca agaaatcctt aagtcaggtt tggatgtcac
     5881 tttccactcc attgcgactg ggaaattgcg tcgctatttc tcttggcaaa atatgctgga
     5941 cgtcttcaaa gttggctggg gaatcgtcca atcgctcttt atcatgttgc gactgcgtcc
     6001 acagaccctt ttttcaaagg ggggctttgt ctcagtaccg cctgttatcg ctgcgcgtgt
     6061 gtcaggagtg cctgtcttta ttcacgaatc tgacctgtct atgggcttgg ccaataaaat
     6121 cgcctataaa tttgcgacta agatgtattc aacctttgaa caagcttcga gtttggctaa
     6181 ggtcgagcat gtgggagcgg tgaccaaggt ttcggacaaa aacactccag aaccagatga
     6241 attggtggat attcaaaccc actttaatcc caaattgcca actgtattgt ttgtcggcgg
     6301 ttctgcaggc gctcgtgtct ttaaccaatt ggtgacagac cataagaaag aactaacaga
     6361 gcgctacaat attatcaatc taactggaga ttctagtctg aatgagttga ggcaaaatct
     6421 ttttcgtgtt gactatgtga ccgatctcta tcaacccttg atggaattgg ctgatattgt
     6481 tgtgacacga ggtggtgcca atacgatttt tgagctcttg gcgatagcaa aattgcatgt
     6541 cattgtgccg cttggtcgtg aagctagtcg tggtgaccag attgaaaatg cagcttactt
     6601 tgttaagaaa ggctatgcag aagaccttca agaaagcgat ttgaccttgg atagtttgga
     6661 agagaagctt tctcacttac taagtcacaa ggaagattac caagctaaga tgaaagcttc
     6721 taaggaattg aaatctctag cagattttta tcaattgttg aaaaaagatt tatcataagg
     6781 aaagtaaatg tcaaaagata agaaaaatga ggacaaagaa accctcgaag aattgaaaga
     6841 gttatcagaa tggcagaaac gaaaccaaga atatctaaaa aagaaggctg aagaagaggc
     6901 ggctctagct gaggagaagg aaaaggaaag acaagctcga atgggagaag aatctgagaa
     6961 gtcagaggat aaacaggacc aggagagtga aacagaccag gaagattcag aatcagctaa
     7021 ggaagagtct gaagaaaaag tagcatcctc agaggctgac aaagagaaag aagagaaaga
     7081 agagaaagaa gaaccagagt ctaaagagaa ggaggaacag gataaaaagc ttgctaaaaa
     7141 ggctacaaag gaaaaaccag ccaaagcaaa gattcctggt atccatatct tgcgagcctt
     7201 cacgatttta tttccaagtc tgcttttatt gtttgtctct gcctacttgc tcagtcctta
     7261 tgcgaccatg aaagatattc gtgttgaggg aacggtgcaa actacagctg atgatattcg
     7321 acaggcttca ggcattcagg attcggatta tacgattaac cttctgctag acaaggcaaa
     7381 atatgaaaag cagattaagt ctaactattg ggttgaatca gctcaacttg tctatcaatt
     7441 tccaactaag ttcactatta aggtcaagga atatgatatt gtggcctact atatttctgg
     7501 tgaaaatcat tatcctattc tttccagtgg tcagcttgag actagttctg tgagtctgaa
     7561 cagtttacca gaaacttatt tatcagttct ctttaatgat agtgaacaaa tcaaagtttt
     7621 tgtctcagaa cttgctcaaa ttagcccaga actcaaggcg gctatccaaa aggtggaatt
     7681 agccccaagc aaggtgacat ccgatttaat tcgattgacc atgaatgatt cggacgaagt
     7741 cttggttcct ctatctgaaa tgagtaagaa actgccatat tacagtaaga ttaagcctca
     7801 attgtcagaa ccgagtgtgg tcgacatgga agctggaatc tacagctaca ctgtggcgga
     7861 taaattaatt atggaagctg aggaaaaagc caaacaagag gccaaggaag ctgagaaaaa
     7921 acaagaagaa gaacagaaaa aacaagagga agagagcaat cgaaaccaaa cgacccagcg
     7981 ttcatcgcgt cgctaggttt accttttctc ttatagttct ttagtgacca tgtttttact
     8041 tgacaagtgc tagtagaaat aatagaataa tagaaaacct ttaaagcagt ccagagaggc
     8101 agctaaggtt agacggtgaa agggtggaga ctacccattt ttcgtggaac cttgctgttg
     8161 gcaggttcct tttttcgtgg cttctgttgg ccagactctc tcactagtaa aggtaaaagg
     8221 agaaacctat gcgagaacat cgtccaatca ttgctcttga ttttcctagt tttgaggcgg
     8281 tcaaggaatt tttagctctt ttctcagcag aagaaagcct ttatctcaag gtagggatgg
     8341 agctttatta cgcagcgggg cctgagattg tgtcctactt aaaaggtttg ggtcatagtg
     8401 tctttttgga tctcaaactt catgacattc ctaatacagt caagtcagcc atgaaggtct
     8461 tgtctcagct tggtgtcgat atgactaatg tccatgcggc tggtggtgta gagatgatga
     8521 aggcggcgcg tgaaggtctt gggagtcaag ccaaattgat cgctgtaacg cagctgacat
     8581 caacgtcaga agcccagatg caggagtttc aaaatatcca aaccagtctg caagagtctg
     8641 tgattcacta tgccaagaag acagctgaag ctggcttgga tggtgttgtt tgctcggctc
     8701 aggaagtaca agtcatcaag caggctacca atccagattt tatctgtctg acaccaggga
     8761 ttcgtccagc tggtgttgca gttggagatc aaaaacgagt catgacacct gctgatgcct
     8821 atcaaatcgg cagtgactat atcgtagtgg gacgtcccat tacccaagct gaggatcctg
     8881 ttgcagctta tcatgccatc aaggatgaat ggacacagga ctggaattaa agaactagat
     8941 tagaaaaata aaaggagaat accatgacac ttgctaaaga tatcgctagc cacctcttga
     9001 aaatccaagc cgtttacctc aaaccagagg aacccttcac ttgggcatct ggtatcaagt
     9061 caccgattta cactgataat cgtgtgacac tagcctatcc agaaactcgt accctaattg
     9121 aaaatggttt tgtggaagct atcaaagaag cctttcctga agtagaagtg attgcaggaa
     9181 ctgcaacagc agggattcca cacggagcca ttattgctga taagatggac ttgccttttg
     9241 cctacatccg tagcaaacca aaagaccacg gagctggtaa tcaaatcgaa ggtcgcgtag
     9301 ctcaaggtca aaaaatggta gtggttgaag accttatttc aacgggtggt tcagttcttg
     9361 aagctgtagc agcagccaag cgagaaggag cagatgtact tggagttgta gcgattttca
     9421 gctaccaatt gccaaaagca gataagaact ttgcagatgc tggtgttaaa cttgtgacgc
     9481 tttcaaacta tagcgagctt atccatctag cccaagaaga aggttacatc acgccagagg
     9541 gccttgatct tctaaaacgc tttaaagaag accaagaaaa ttggcaagaa ggttaggtca
     9601 gtaagataaa gagagacgag gctaccgagt ctc
//
LOCUS       DAVGJL010000081         9569 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.081, whole genome shotgun sequence.
ACCESSION   DAVGJL010000081 DAVGJL010000000
VERSION     DAVGJL010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9569
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.081"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            147..380
                     /locus_tag="VVE42_001493"
     CDS             147..380
                     /locus_tag="VVE42_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComC/BlpC family leader-containing
                     pheromone/bacteriocin"
                     /protein_id="HEU3073672.1"
                     /translation="MDTKMMSQFAVMDNEMLARIEGGDVSDIYRGYANQVSPFGSYPP
                     ILKNSGPFPVSGYCPRGYHDRGYIGAGFHLCGI"
     gene            412..1101
                     /locus_tag="VVE42_001494"
     CDS             412..1101
                     /locus_tag="VVE42_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU3073673.1"
                     /translation="MKKYQLLFKISAVFSYLFFVFGLSQMTLIIQNYWQFSSQIGNFV
                     WIQNFLSLLFSGVMIWILVKTGHGYLFHIPRKKWLWYSILTVLVVVLQISFNVQTAKH
                     VQSTAEGWAVLIGYSGTNFAELGIYVTLFFLTPLMEELIYRGLLQHAFFKHSRFGLDL
                     LLPSILFALPHFSSLPSLLDIFVFATFGIIFAGLTRYTKSIYPSYAVHVINNIVATFP
                     FLLTFLHRVLG"
     gene            1141..1374
                     /locus_tag="VVE42_001495"
     CDS             1141..1374
                     /locus_tag="VVE42_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073674.1"
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFIGAGYVVGFWLLILNENQRAN"
     gene            1525..2136
                     /locus_tag="VVE42_001496"
     CDS             1525..2136
                     /locus_tag="VVE42_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU3073675.1"
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGDFFQNESALIIVSL
                     LVTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHL
                     AEVSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGGLLLGLAYKKSQNLWVSTLVHMFYNSWPLLYYL"
     gene            2297..3091
                     /locus_tag="VVE42_001497"
     CDS             2297..3091
                     /locus_tag="VVE42_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEU3073676.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            3088..3723
                     /gene="trmB"
                     /locus_tag="VVE42_001498"
     CDS             3088..3723
                     /gene="trmB"
                     /locus_tag="VVE42_001498"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEU3073677.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEVGVPNIKLLWVDGSDLTD
                     YFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            3849..4328
                     /gene="rimP"
                     /locus_tag="VVE42_001499"
     CDS             3849..4328
                     /gene="rimP"
                     /locus_tag="VVE42_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEU3073678.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEMISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDELTMEYMDKTRKKTVQIPYSLVSKARLAVKL"
     gene            4372..5508
                     /gene="nusA"
                     /locus_tag="VVE42_001500"
     CDS             4372..5508
                     /gene="nusA"
                     /locus_tag="VVE42_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEU3073679.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     CGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            5530..5823
                     /locus_tag="VVE42_001501"
     CDS             5530..5823
                     /locus_tag="VVE42_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEU3073680.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            5816..6115
                     /locus_tag="VVE42_001502"
     CDS             5816..6115
                     /locus_tag="VVE42_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEU3073681.1"
                     /translation="MNKQKISNLLGLAQRAGRIISGEELVVKAIQDGKAKLVFLAHDA
                     GPNLTKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            6132..8924
                     /gene="infB"
                     /locus_tag="VVE42_001503"
     CDS             6132..8924
                     /gene="infB"
                     /locus_tag="VVE42_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEU3073682.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKPAAAPKVEAKPAAPKVSAEKKAEKSEPAKPAVAKEEAKPAEPVAPKTEKVA
                     AKPQSRNFKAEREARAKEQAERRKQNKGNNRDQQQNGNRQKNDGRNGGKQGQSNRDNR
                     RFNDQAKKQQGQQKRRNERRQQEDKRSNQAAPRIDFKARAAALKAEQNAEYARSSEER
                     FKQYQAAKEALAQANKRKEPEEIFEEAAKLAEQAQQVQAVVEVVPEKKEPAVDTRRKK
                     QARPDKNRDDYDHEEDGPRKQQKNRSSQNQVRNQKNSNWNNNKKNKKGNNKNNRNQTP
                     KPVTERKFHELPTEFEYTDGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGET
                     IELLMVDYGIEAKQKVEVDNADIERFFVEDGYLNEDELVERPPVVTIMGHVDHGKTTL
                     LDTLRNSRVATGEAGGITQHIGAYQIVENGKKITFLDTPGHAAFTSMRARGASVTDIT
                     ILVVAADDGVMPQTIEAINHSKAANVPIIVAINKIDKPGANPERVIGELAEHGVMSTA
                     WGGDSEFVEISAKFNQNIEELLETVLLVAEIQELKADPTVRAIGTVIEARLDKGKGAV
                     ATLLVQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGD
                     HFAVYEDEKSARAAGEERAKRALMKQRQATQRVSLENLFDTLKAGELKSVNVIIKADV
                     QGSVEALSASLQKIDVEGVKVTIVHSAVGAINESDVTLAEASNAFIVGFNVRPTPQAR
                     QQAEADDVEIRLHSIIYKVIEEMEEAMKGMLDPEFEEKVIGEAVIRETFKVSKVGTIG
                     GFMVINGKVARDSKVRVIRDGVVIYDGELASLKHYKDDVKEVTNGREGGLMIDGYNDI
                     KMDDVIEAYVMEEIKR"
     gene            9175..9525
                     /gene="rbfA"
                     /locus_tag="VVE42_001504"
     CDS             9175..9525
                     /gene="rbfA"
                     /locus_tag="VVE42_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEU3073683.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMLGD
                     LSVAKVYYTILSNLASDNQKAQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
BASE COUNT         2931 a         1794 c         2215 g         2629 t
ORIGIN      
        1 tgctttcttt ttcgactatt ttaacgcctc aagatgtttt gatgacaatt caagatttga
       61 tgaaaaaaat taataaacga tgatatacta aacttgtcaa ggttgcaaca ggacaaaaat
      121 taaacataaa aaaggagata ttcgtcatgg atacaaaaat gatgtcacaa tttgcagtta
      181 tggataatga aatgcttgct cgtatagagg gtggggatgt atctgatatt tatagagggt
      241 acgctaatca ggtaagtcca ttcggttctt accctcctat attaaaaaat tcaggtcctt
      301 ttccagtaag tggttactgc ccgcgcggtt atcatgaccg tggttatata ggagcaggtt
      361 ttcatttatg tggaatatag gctgaaatac aagaaatgag gaagaatttc tatgaaaaag
      421 tatcaacttc tattcaaaat aagtgcagtt ttctcttact tatttttcgt atttggtctt
      481 tctcagatga cgcttattat tcaaaattat tggcaatttt cttcccagat tggcaatttc
      541 gtctggattc aaaatttctt gagtttgcta tttagcggag tcatgatttg gattctggtt
      601 aagacaggtc atggttatct ctttcacatt ccaagaaaaa aatggctttg gtattcgatt
      661 ttgacagtat tagtggtagt gctccagatc tcttttaacg ttcagacagc taaacatgtt
      721 cagtcaactg ctgaaggttg ggctgtattg atcggttata gtgggaccaa ctttgctgag
      781 ctaggtatct atgtaaccct gtttttcctg actccactaa tggaagagtt gatttatagg
      841 ggattacttc aacacgcctt ctttaagcat tcgcgatttg gccttgattt gcttcttcct
      901 tccattttgt ttgctcttcc tcatttttca agcctaccta gtttgttaga tattttcgtc
      961 tttgcaacat ttggaatcat ctttgccggt ttgacccgct ataccaagag tatttatcca
     1021 tcctatgcgg tgcatgtgat caataatatt gtagcgacct tcccgttttt gctcactttt
     1081 ctacataggg tcttggggta aaaaaacaaa aggtgctttt cagccataga ggaggtcatc
     1141 atgtataaac acttattttt cctagattcc aaaactttag atcggttgac accctatatt
     1201 ctagtcttgg cttctgacac cattgctttt aatgtttttg tgctaacctt tgtatctgcg
     1261 gtggttttta atttcctaaa ttccatgcta gctttaatgg ctatattcat aggggctggc
     1321 tatgtggtcg gattttggtt actaatactc aatgaaaatc aaagagcaaa ctaggaaact
     1381 agccgcaggc tgtacttgag tacggcaagg cgacgttgac gtggtttgaa tttgattttc
     1441 gaagagtata aaatggtttg ttttggaaag gctagaacta aagaatgacg tgtaggaagt
     1501 ctgtttgttg aggaggataa ttttatggag ttttttgata aatttcatgc cttgtgtttt
     1561 ggatttttag tactaataat tgtcattaca gttccttata cgattaacca tggggatttt
     1621 tttcaaaatg aatctgcatt gattattgta agtcttcttg taacctcgct gagtgttgct
     1681 tatgctagaa agtttgaaat gatttctttt gggatgttaa gcaagaaaca acttttgctt
     1741 ttcattgcaa tctttcttct aagtgtactt gagacgctgg tttatattca tttcttcgct
     1801 gtttcttctg gctcaggggt ccaacacttg gcggaagtca gcagaggaat ttccctgtct
     1861 ttgattttga ctacctcagt ttttggcccc atccaggagg aactcatttt cagaggactt
     1921 cttcaaggtg cggtttttga caattcttgg ttagggcttg tgctaacttc ctctctcttt
     1981 tctttcatgc atggaccttc taatgtccct tcgtttattt tttatctact tgggggcttg
     2041 ttgctgggcc ttgcttataa aaagagccaa aacctatggg tttctactct agtccacatg
     2101 ttttacaaca gttggccact cctatattat ttataaaaat tatgaaaagg taagcagaag
     2161 acggtgctta cctttttcaa gctataatga tacccaagcc aatccagagg cttttctttg
     2221 agaatcgggt tagagtggtt gacgaatagg ccaaaaacta gtagaatagt aaggaaactt
     2281 tatacggagg aaagaaatgg atttgggtga taatgagcta acactgactc ccatacctgg
     2341 gaaaagtggc aaggcttata tgggtagcta tcctgatggg aagcgcatct ttgtaaaaat
     2401 gaacacctct ccaatcctac ctggtctagc tagagaacaa attgctccac aattattatg
     2461 gagtcgtcgt ttggcagatg ggcgtgatat gtgtgctcaa gaatggttga caggcaagat
     2521 attgaccccc tatgatatga atcgtaagca aatcgtcaat attttaaccc gtcttcatcg
     2581 ctcacgtccg ttgatgacac aattgagtcg tttgggctat gccatggaaa cacctgtaga
     2641 tttactacag tcttggcaag aaacggctcc agatgctttg cgtaaaaatc attttatcag
     2701 tgaagtgatg gctgatttgc gtcagactat tccaggattt agagaggacc atgcgaccat
     2761 tgtccatgga gatgtacgac atagtaattg gattgagaca gatagtggct tgatttattt
     2821 agtagattgg gattcggttc gcttgaccga tcgcatgttt gatgtggccc atatgctctg
     2881 ccattatatt tcagaacatc agtggaagga atggttgacc tactacggtt acaagtacaa
     2941 tcaaacggta ttaagtaaat tgtattggta tggtcaattg tcttatttga gtcagatttc
     3001 caagtattat atgaaccaag atttagaaaa tgtcaatcgg gagattcatg gtttgcgtca
     3061 tttccgagac aagtatggaa agagaagatg agagttagaa atcgtaaagg ggcaacagaa
     3121 ttactagagg caaatcccca gtatgtggtc ctcaatccct tggaagccaa ggcaaaatgg
     3181 cgggacttgt ttggcaatga taatcccatt catgtggaag ttggaagtgg aaagggtgcc
     3241 tttgtttcag gtatggccaa gcaaaaccct gacatcaact atatcgggat tgatattcaa
     3301 aagtctgttt tgagctacgc tttggacaag gtgcttgaag ttggagtgcc taacatcaag
     3361 ctcttgtggg tagatggttc tgacttgact gactactttg aagacggtga gattgatcgc
     3421 ttgtatctga acttttcaga tccatggccg aaaaaacgcc atgaaaagcg tcgtttgacc
     3481 tacaagacct ttttggatac cttcaaacgt atcttgcctg aaaatggaga aattcatttc
     3541 aagacggata accgtggctt gtttgagtac agtttagtga gcttttctca atatggcatg
     3601 aaactcaatg gtgtctggct agatttgcat gccagtgatt ttgaaggcaa tgtcatgaca
     3661 gaatacgagc aaaaattctc aaacaagggg caagttatct accgagttga ggcagaattt
     3721 taagagatag cctaaaatta ggctgtacaa gtgcttttgc tttacataag ttggcaaacg
     3781 tgctatactg atagtaagaa tatgaaaagt gaggcgggga aatatcttcg cctcttgctt
     3841 atgaggaggt ggacgcaatc gcaacaatcg tagaattagt cagagaagtt gtagaacctg
     3901 tcatagaagc tccttttgaa ctcgtggata tcgagtatgg aaagattggc agtgacatga
     3961 ttctcagtat ttttgtagat aaacccgaag gaattacctt gaacgacacg gcagacttga
     4021 cagaaatgat cagtcctgtc ctagacacca tcaagccaga tcccttccca gaacaatatt
     4081 tcctagaaat taccagtcca ggtttggaac gtcctttgaa aaccaaggat gccgtcgctg
     4141 gagcggttgg aaaatacatc catgtcgggc tctaccaagc catcgataag caaaaggtct
     4201 ttgaaggaac cttgttggcc ttcgaagagg acgagttgac tatggaatat atggacaaga
     4261 cgcgtaagaa aaccgtccaa attccataca gtttagtatc aaaagcacgt ttagcagtta
     4321 aattatagaa aaagaaagga tagcttttga ggattcaaaa gtgaagaaaa catgagtaaa
     4381 gaaatgctag aggccttccg cattttggaa gaagacaagg gaatcaaaaa agaagatatc
     4441 atcgacgcag tagtagagtc gcttcgttcc gcttatcgca gacgctatgg tcagtcagac
     4501 agcgtagcta ttgacttcaa cgaaaaaaca ggtgacttta cagtttatac tgtccgtgaa
     4561 gttgttgatg aagtatttga tagccgtttg gaaatcagct tgaaagatgc tcttgccatt
     4621 aattcagctt atgaacttgg agacaaaatc aagtttgaag aagcaccagc tgagtttggt
     4681 cgtgtagcag cccaatctgc caaacaaacc atcatggaaa aaatgcgcaa gcaaacacgt
     4741 gccatcactt acaatactta caaagaacat gagcaagaaa tcatgtctgg tacagtagaa
     4801 cgctttgaca accgctttat ctatgtcaac cttggtagca tcgaagccca attgtcaaaa
     4861 caagaccaaa ttcctggaga agtttttgct tctcatgatc gtatcgaagt ttatgtttac
     4921 aaggttgaag acaaccctcg tggtgtgaac gtctttgtta gccgtagcca tccagaaatg
     4981 atcaaacgtt tgatggagca agaaattcca gaagtttatg atggaactgt tgaaatcatg
     5041 agcgtggctc gtgaagcagg tgaccgtacg aaggttgctg ttcgtagcca caatccaaac
     5101 gtggatgcta tcggtacaat cgttggacgt ggtggtgcta atatcaagaa gattactagc
     5161 aaattccacc cagctcgtta cgatgctaaa aatgaccgca tggtaccaat cgaagaaaat
     5221 atcgatgtta tcgagtgggt agcagatcca gctgaattta tctacaatgc catcgctcct
     5281 gctgaggttg accaagttat ctttgatgaa aacgacagca aacgtgcctt ggtggttgtt
     5341 ccagataaca agctttctct tgccattggt cgttgtggac aaaacgtgcg cttggcggct
     5401 cacttgactg gttaccgtat cgatatcaag tctgctagcg aatttgaagc catggaagac
     5461 gctgcttcag tagagttgga agtagaaaac gatactgtag aagaataaaa gctgctagag
     5521 gagggaaaga tgaaaacaag aaaaatccct ttgcgcaagt ctgttgtgtc taacgaagtg
     5581 attgataagc gtgatttgct ccgcattgtc aagaacaagg aaggacaagt ctttattgat
     5641 cctacgggca aggccaatgg ccgcggcgct tatatcaaac tagacaatgc agaagcccta
     5701 gaggcgaaaa agaagaaggt ctttaaccgc agctttagca tggaagtgga agaaagcttt
     5761 tatgacgagt tgatcgctta tgtggatcac aaagtgaaaa gaagagagtt gggacttgaa
     5821 taagcaaaag ataagtaatc tcttggggct tgctcagcga gcagggcgca tcatatcggg
     5881 tgaagaattg gtggtcaagg ccattcaaga cggcaaggcc aagttggtct ttctagctca
     5941 tgatgctgga cccaatctga ccaagaagat tcaagataaa agtcattatt atcaagtaga
     6001 aattgtaacc gtgttttcaa cactggaatt aagcatagca gtcgggaaat cgagaaaggt
     6061 tttggctgta acagatgctg gatttacaaa gaaaatgagg tctcttatgg aatagaagag
     6121 gaggacatga tttgtctaag aaaagattgt acgaaatcgc aaaagaactt ggaaaagaaa
     6181 gtaaagaagt tgtagcgcgt gcaaaagagt tgggcttgga tgtgaaaagc cactcatcaa
     6241 gtgtggaaga agctgtcgct gcaaaaattg ctgccagctt taagcctgca gctgctccga
     6301 aagtagaagc aaaacctgca gcaccaaaag taagtgcaga aaagaaagcc gaaaaatctg
     6361 agccagctaa accagctgta gctaaggaag aggcaaaacc ggctgagcca gttgctccga
     6421 aaacagaaaa agtagcagcg aaaccgcaaa gtcgtaattt caaggctgag cgtgaagccc
     6481 gtgctaaaga gcaggcagaa cgacgcaagc aaaataaggg caataaccgt gaccaacaac
     6541 aaaacggaaa ccgtcagaaa aacgacggcc gtaatggtgg aaaacaaggt caaagcaacc
     6601 gcgacaatcg tcgctttaat gaccaagcta agaagcagca aggtcagcaa aaacgtagaa
     6661 atgagcgccg tcagcaagag gataaacgtt caaatcaagc ggctccacgt attgacttta
     6721 aagcccgtgc agcagcccta aaagcagagc aaaatgcaga gtacgctcgt tcaagtgagg
     6781 aacgcttcaa gcagtatcag gctgctaaag aagccttggc tcaagctaac aaacgcaagg
     6841 aaccagagga aatctttgaa gaagcggcta agttagctga acaagcacag caagttcaag
     6901 cagtggttga agtcgtccct gagaaaaaag aacctgcagt ggatacacgt cgtaaaaaac
     6961 aagctcgacc agacaaaaat cgtgacgatt atgatcatga agaagatggt cctagaaaac
     7021 aacaaaagaa tcgaagtagt caaaatcaag tgagaaatca aaagaatagt aactggaata
     7081 acaacaaaaa gaacaaaaaa ggcaataaca agaacaaccg taatcagact ccaaaacctg
     7141 ttacggagcg taaattccat gaattgccaa cagaatttga atatacagat ggtatgaccg
     7201 ttgcggaaat cgcaaaacgt atcaaacgtg aaccagctga aatcgttaag aaacttttca
     7261 tgatgggtgt catggccaca caaaaccaat ccttggatgg ggaaacaatt gaactcctca
     7321 tggtggatta cggtatcgaa gccaaacaaa aggttgaagt ggataatgct gacatcgaac
     7381 gtttctttgt cgaagatggt tatctcaatg aagatgaatt ggttgagcgt ccaccagttg
     7441 ttactatcat gggacatgtt gaccacggta aaacaaccct tttggatact cttcgtaact
     7501 cacgtgttgc gacaggtgaa gcaggtggta ttactcagca tatcggtgcc taccaaatcg
     7561 tggaaaatgg taagaagatt accttccttg atacaccagg acacgcggcc tttacatcaa
     7621 tgcgtgcgcg tggtgcttct gttaccgata ttacgatctt ggtcgtagcg gcagatgacg
     7681 gggttatgcc tcagactatt gaagccatca accactcaaa agcagctaac gttccaatca
     7741 tcgtagccat taacaagatt gataaaccag gtgctaaccc agaacgcgtt atcggtgaat
     7801 tggcagagca tggtgtgatg tcaaccgctt ggggtggaga ttctgaattt gttgaaatct
     7861 cagctaaatt caaccaaaat atcgaagaat tgttggaaac agtccttctt gtagctgaaa
     7921 tccaagaact caaggcagac ccaacagtgc gtgcaatcgg tacggttatc gaagcgcgct
     7981 tggataaagg aaaaggtgcg gtcgcaaccc ttcttgtaca acaaggtacc ttgaatgttc
     8041 aagacccaat cgttgtcgga aataccttcg gtcgtgtccg tgctatgacc aacgaccttg
     8101 gtcgtcgtgt taaagttgct ggaccatcaa caccagtctc tatcacaggt ttgaacgaag
     8161 caccgatggc gggtgaccac tttgccgttt acgaggatga aaaatctgcg cgtgcagcag
     8221 gtgaagagcg tgccaaacgt gccctcatga aacaacgtca agctacccaa cgtgttagcc
     8281 ttgaaaacct ctttgatacc cttaaagctg gggaactcaa atctgttaat gttatcatca
     8341 aggctgatgt acaaggttct gttgaagccc tttctgcctc acttcaaaag attgacgtgg
     8401 aaggtgtcaa agtgactatc gtccactcag cggtcggtgc tatcaacgaa tcagacgtga
     8461 cccttgccga agcttcaaat gcctttatcg ttggtttcaa cgtacgccct acaccacaag
     8521 ctcgtcaaca agcagaagct gacgatgtgg aaatccgtct tcacagcatt atctacaagg
     8581 ttatcgaaga gatggaagaa gctatgaaag ggatgcttga tccagaattt gaagaaaaag
     8641 ttattggtga agcggttatc cgtgaaacct tcaaggtgtc taaagtggga actatcggtg
     8701 gatttatggt tatcaacggt aaggttgccc gtgactctaa agtccgtgtt atccgtgatg
     8761 gtgtcgttat ctatgatggt gaactcgcaa gcttgaaaca ctataaagac gacgttaagg
     8821 aagttacaaa cggccgtgaa ggtggattga tgatcgacgg ctacaatgat attaagatgg
     8881 atgatgtgat tgaggcgtat gtcatggaag aaatcaagag ataagatttt ttgctccttt
     8941 cttaggtggt gagggacgca agcaaaccga tggtttcatt gcttattttt gagcctaggg
     9001 tctcaaaaat cccctgtgat gggactgata aatcagttcc atcactttca ccacggcgaa
     9061 agaagcagat gacttcaaat tgaacttcgt ttcaatttaa actgaaaatc aagaagttta
     9121 aaatagctag gtctgctggc ctagcttttg gttcaaagta gagaaaggaa tatcatggca
     9181 aatcatttcc gtacagatcg tgtgggcatg gaaatcaagc gtgaagtcaa tgagattttg
     9241 caaaagaaag tccgtgatcc acgtgtccaa ggtgtgacca tcacagatgt tcagatgctg
     9301 ggtgacttgt ctgttgccaa ggtttattac accattttga gtaaccttgc ttcggataac
     9361 caaaaagccc aaatcgggct tgaaaaagca actggtacca tcaaacgtga acttggtcgc
     9421 aatttgaaat tgtacaaaat cccagatttg accttcgtca aagacgagtc catcgagtat
     9481 ggaaacaaga ttgacgagat gctacgcaat ctggataaga actaaagaag aggggttgcc
     9541 cctctttttt ggtggaggaa aataggttg
//
LOCUS       DAVGJL010000082         9343 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.082, whole genome shotgun sequence.
ACCESSION   DAVGJL010000082 DAVGJL010000000
VERSION     DAVGJL010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9343
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.082"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            10..1230
                     /locus_tag="VVE42_001505"
     CDS             10..1230
                     /locus_tag="VVE42_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEU3073684.1"
                     /translation="MLTVSLLGGASTAVAQDFTIAAKHAIAVEANTGKILYEKDATQP
                     VEIASITKLITVYLVYEALENGSITLSTPVDISDYPYQLTTNSEASNIPMEARNYTVE
                     ELLEATLVSSANSAAIALAEKIAGSEKDFVDMMRAKLLEWGIQDATVVNTTGLNNETL
                     GDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTITSTNYML
                     EGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNADHQDNNPYARFTATS
                     SLMDYISSTFTLRKIVQQGDAYQDSKTPVQDGKEDTVTAVAPEDIYLIERVGNQSSQS
                     VQFTPDSKAIPAPLEAGTVVGHLTYEDKDLIGQGYITTERPSFEMVADKKIEKAFFLK
                     VWWNQFVRFVNEKL"
     gene            complement(1461..2873)
                     /gene="sufB"
                     /locus_tag="VVE42_001506"
     CDS             complement(1461..2873)
                     /gene="sufB"
                     /locus_tag="VVE42_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEU3073685.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSA
                     AKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFDDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(2927..3367)
                     /locus_tag="VVE42_001507"
     CDS             complement(2927..3367)
                     /locus_tag="VVE42_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEU3073686.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQISLNNPTCGDVI
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDAVLGKTKQEILELATIFSEMVQGQ
                     KDERQGQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            complement(3354..4580)
                     /locus_tag="VVE42_001508"
     CDS             complement(3354..4580)
                     /locus_tag="VVE42_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU3073687.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKAINS
                     YYEQDNANVHRGVHTLAERATASYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR
                     FAEEILTEGDQVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHASNVLGVINPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQFASWKELPWKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAVEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLEVPATARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            complement(4591..5853)
                     /gene="sufD"
                     /locus_tag="VVE42_001509"
     CDS             complement(4591..5853)
                     /gene="sufD"
                     /locus_tag="VVE42_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEU3073688.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIERVKFH
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     DSNVPFNKHIMIIAGKNSKISYLERLESRGEGSDKVTANITVEVIARSGAQVKFAAID
                     RLGENVTAYISRRGKLGNDASIDWAIGVMNEGNVVADFDSDLIGNGSHADLKVVALSS
                     GRQVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            complement(5881..6651)
                     /gene="sufC"
                     /locus_tag="VVE42_001510"
     CDS             complement(5881..6651)
                     /gene="sufC"
                     /locus_tag="VVE42_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEU3073689.1"
                     /translation="MSVLEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEDDEKISVREFITKLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            complement(6801..6995)
                     /locus_tag="VVE42_001511"
     CDS             complement(6801..6995)
                     /locus_tag="VVE42_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEU3073690.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
     gene            complement(7023..8678)
                     /gene="dnaX"
                     /locus_tag="VVE42_001512"
     CDS             complement(7023..8678)
                     /gene="dnaX"
                     /locus_tag="VVE42_001512"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEU3073691.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYAPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIKEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTAISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTDLLHYLRDLLIVQTGGENTHHSSVFVENLALPQKNLFEMIRLATVNLADIK
                     SSLQPKIYAEMMTVRLAEIKPEPALSGAVENEIATLRQEVARLKQELSNAGAVPKQVA
                     PAPSRPATGKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLVGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQTEKEVEESLIPEGFEFLADKVKVEED"
     gene            complement(8678..9175)
                     /locus_tag="VVE42_001513"
     CDS             complement(8678..9175)
                     /locus_tag="VVE42_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU3073692.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTTWDFTM
                     FEEKS"
BASE COUNT         2730 a         2086 c         1761 g         2766 t
ORIGIN      
        1 tttttaactt tgttaactgt ctctctttta gggggtgctt ctactgctgt tgctcaagat
       61 tttaccattg ccgctaaaca tgcgattgct gttgaggcaa atactggtaa aattctctat
      121 gagaaggatg caacgcaacc tgtcgaaatt gcttccataa caaaattgat tactgtttat
      181 ctggtctatg aagctttgga aaacggaagt attaccctct ccactcctgt agatatttct
      241 gattatcctt atcaattgac gacaaattct gaagccagta atattcctat ggaggcccgt
      301 aattatactg tcgaagagtt gcttgaagca actctggtat ctagtgccaa cagcgccgct
      361 attgccctag ctgagaaaat tgctggctca gaaaaagatt tcgtcgatat gatgcgggca
      421 aaactcttgg aatggggaat tcaggatgcc actgttgtca atacgacagg tcttaacaat
      481 gaaactctag gggataacat ttacccaggt tctaaaaaag atgaggaaaa taagcttagt
      541 gcttatgatg tcgctatcgt tgctcgcaac ctcatcaaaa aatacccaca agtcttagaa
      601 atcaccaaaa aaccttcttc tacttttgct gggatgacaa tcacttcaac caactacatg
      661 ttagaaggta tgcctgctta ccgtggtggt tttgatgggc taaaaacagg aacaacagat
      721 aaggctggag agtcttttgt tggtactact gtcgaaaaag gcatgagagt catcacagtt
      781 gttttaaatg cagatcatca agacaataat ccttacgctc gatttacagc tacatcttcc
      841 ctaatggatt atatttcttc tacatttaca cttcgcaaaa tcgttcaaca aggcgatgcc
      901 tatcaagata gcaaaacccc tgtacaagat ggaaaagaag atacagtaac tgcagtggct
      961 ccagaggata tctatctaat cgaacgtgtt gggaatcaat cttcccaatc tgttcaattc
     1021 acacctgatt ccaaagcaat cccagcacca cttgaagctg gaacagtggt tggccatttg
     1081 acttatgaag acaaggactt gattggtcaa ggttacatca ccacagagcg ccctagtttc
     1141 gaaatggtag cagacaagaa aattgaaaaa gccttcttct taaaagtttg gtggaatcag
     1201 tttgtccgct ttgttaacga gaaattataa aaaatcgcga ttttaaaccg cgattttttg
     1261 atacaagttc atcttcaaaa tccttgattt tgagtaaatc taatactcaa aatcaaagaa
     1321 caaactagga agctagccgc agcttgctca aaacactgtt ttgaggttgt agatgaaact
     1381 gacgaagtca gtaaccatac atacggtaag gcgatgctga cgtggtttga agagattttc
     1441 gaagagtata aaatcaaatt ttatccaact gatccctcca tttcatagct aatcaagcgg
     1501 ttcagctcaa ctgcgtattc cattggaagt tcttttgtaa agggttctac aaatcccatg
     1561 acaatcattt cagttgcctc agattctgac aatccacggc tcatgagata atacaattgc
     1621 tcttctgaaa tcttagatac tttggcttcg tgttccaaag ccacttgcga gttgtgaatt
     1681 tcattaaatg gaatagtatc tgatgctgac aagtcatcca tgataatggt atcacattca
     1741 atgtgggaaa cagatttctt agagttcttg ttaaaggtga cttgtccacg gtagtcaacc
     1801 tttcctccac ctttagcgat ggatttagac acaatagacg agctggtatg tggagcattg
     1861 tgaatcatct tagcacccgt gtcttggtgt tgccctgcat tagcaaaggc gatagagagc
     1921 atggtaccac gcgctccttc tccatcaagg taaacagatg gatatttcat agtcgttttg
     1981 gcacccaagt ttccatcaat ccactcaaca gtggcatcct tttgagcctt agcacgcttt
     2041 gttaccaagt tatagacgtt atcagaccag ttttggatag ttgtataacg catataagct
     2101 ccgtccaaag caaaaatttc tacaatggca gcgtgtaagc tattgcttga atatgttggt
     2161 gctgtacatc cttctacgta gtggacgctt gctccctcat caacgataat caaggtacgt
     2221 tcgaactgac ctatattttc gttattgata cggaaataag tttgaagtgg aatatctacc
     2281 ttgacacctt ttggcacgta gataaaagtt ccacccgacc atactgctga gttgagggct
     2341 gccaacttgt tatctgtcgg cggtaccaac ttcgcaaagt attgtttaaa taagtctggg
     2401 tattccttga gtgcggaatc tgtatctgta aagataatac ctaatttttg gaactcttcc
     2461 ttcatgttgt ggtaaaccac ttctgactcg tactgggcag aagcccctgc taaataagca
     2521 cgttcagctt ctggaatccc gatacgttca aaggtttctt taatcttttc aggtacatca
     2581 tcccaagaac gggctggttt gtcagatggt ttttggtagt agattaagtc atcaaagtca
     2641 atctctgaca agtctgctcc ccaagtttgc atgggcattt ttttgaaggt ttcataagac
     2701 ttcaaacgga actccaacat ccactcaggc tcacccttag cagcagataa ttcacgaata
     2761 acaccttcgt tgagtccttt tcctgtcgat aagacaggct ctacatcgtc atggaaacca
     2821 aatttatatt caccaaggtc aattggtttt ggttctactc tttcttcagc catagtatcc
     2881 tttctttctt catttctaat aattcataag acaaaagaaa cttgtcttac tgtttttctt
     2941 gattttcaat tgttttctta agggcattcc aagctagggt tgcacacttg attctttgag
     3001 ggaatttggc aacacctgac aagaatgccg cgtctccaag ttggccttga cgctcatctt
     3061 tttgcccttg aaccatttca gaaaaaatag tcgccagttc taaaatttct tgtttggttt
     3121 ttcctaaaac ggcatctgtc atcatactag cagaagcagt tgaaatcgtg catcctgaat
     3181 ttagaaaagc aatatcttcc aaacggtcct ctgcatcaaa cttgacagag aggttgatga
     3241 catccccaca agtcggattg ttgagactga tttgctcagc atcttctaac ttcccttggt
     3301 gatgtggatt tttcgaatgg tctgctacca ctgccatata aaggctatct agtttagaaa
     3361 gtgccattga aaaactcctt tgtcttttgt agggcatcga ctagtttgtc gcaatctgcc
     3421 ttggtattgt agatataaaa acttgcacga gctgttgctg ggacttccaa atactgaagc
     3481 aagggttgcg cacaatggtg accagcacga acagccactc cttcataatc cagagccgtc
     3541 gcaagatcgt gaggatggag atcacctagg ttaaaggcaa taacacccga acgttgagcc
     3601 aaatcctgag aaccgtaaat ggtcaatccc tcaattgcct gcagttttgg atagacgtac
     3661 gcaatcaatt cctgttcatg agcttcaacg gcatccatac caatcttttc caaataatca
     3721 actgcagtcg caagtccaat agctcctgcc atatttggcg ttccagcctc aaatttccaa
     3781 ggcaattcct tccaactagc aaattgctcg tagacaaaat caatcatctc gccgccaaat
     3841 tctactggag acatttgctc aagatacttt tctttgccgt aaaggacacc gataccagtc
     3901 ggaccagcca tcttgtgacc cgaaaaggcg aaaaagtcca gatccaagtc ctggacatca
     3961 atcttcatat gaggtgtaga ttgagcacca tccactacca tgattgcccc aacttggtgg
     4021 gctaattgag tgatttcctt gatcggattg atcacaccaa gaacattgga ggcatgagct
     4081 agggaaacaa atttaacctt atcagtcaat ttagctcgca aatcctccat atccaaggca
     4141 ccgtctttaa gatagacata gacaagctct gctccagtct ttcgacaagc ttcctgccat
     4201 ggaatgatat tagaatggtg ttccattact gaaatcaaga cctggtctcc ctcagtgaga
     4261 atttcctcag caaagcgtgc cacccagtta aggctggttg tcgttcctct ggtaaagaga
     4321 acttcctttg tagagcctgc attaataaac ttacgaatgg tttcacgagc agcttcataa
     4381 gaagctgtcg ctcgttccgc taaggtatgg acaccacggt gaacattggc attgtcctgc
     4441 tcatagtaac tgttaatagc tttcagaact actaggggtt tttgtgtcgt cgcagcattg
     4501 tccagataga ccagaggttc atcattgaca atctgatcta aaattggaaa atccttgcga
     4561 atcgcttcta catctaacat aggcttcccc ttagcgtttt gacaatttct cttcgatagt
     4621 tgcaatcatt tcatcacgaa cttccttgac tggaatctcc acgataacag atccaaggaa
     4681 accacgaaca accaaacgct ctgcagttgc cttatccaat ccacgactca tgaggtaata
     4741 catgtcttct ggatcaactt gtccgataga cgctgcgtgt cctgcagtga catcattttc
     4801 atcaatcaaa agaattgggt tagcatctga acgcgcttgg tctgaaagca tgagaacacg
     4861 gctctcttgt tgcgcatctg ctcccttagc acccttgatg atgtggccga taccattgaa
     4921 agtcaaagtt gctttttcaa ggataacccc atgttgtagg atatttccga tagagttgca
     4981 gccatagtta gttacacgag tatcaatccc ttgtacctga cgaccacttg aaagagctac
     5041 aaccttgagg tcagcatggc taccattacc aatcaagtca ctatcaaaat cagcaacgac
     5101 atttccttcg ttcatgacac cgatagccca gtcaatactt gcatcgttgc ctaatttacc
     5161 acgacggcta atgtaggcag tgacgttttc acctagacgg tcgatagcag caaacttgac
     5221 ttgcgcacca gaacgtgcaa tcacttccac tgtgatattg gcagttactt tgtcacttcc
     5281 ttcaccgcgt gactctaaac gctccagata actaatctta gaatttttac cagcgataat
     5341 cataatatgc ttgttaaacg gcacattgct atcgctatct tggtagaaaa ttccttcaat
     5401 tggctctgtg atttctacgt tatctggaat atagagtaca gcaccactgt taaagtaagc
     5461 tgtgtggtaa gccgccaact tgtcatcatc atacttaaca gatgacatga agaattcttc
     5521 gatcagctct ggaatttctt ctaaagctga gtgaaagtct gtgaagacaa caccctgttc
     5581 agctaactca actggagttt gttcgaaaac agtttgagtt cctacttgca ccaacttcaa
     5641 gtgatgatct aaagctgtga aatctggaac atttgctgat ggctcatttt ctgtaatcgt
     5701 tccatcaccc agattccaac ggtggaattt gacacgctca ataactggta attccaaagt
     5761 ctcaatcttg tcaaaagctt tttgacggag atcagccaac cagcttggtt cagcgtgcat
     5821 ttctgaaaaa agtttaatat tttctctagt catttacttc tcctaaaaga tacgaggaaa
     5881 ttacaattct tccttgtagt cgtagccaag ttcttcagct aattttgcgt atccttcacg
     5941 ttccaaacgc gcagccaatt ctggaccacc agaaaggaca acacgacctt ccatcatcac
     6001 gtgtaccaca tcaggtgtga tatagttcaa aagacgttgg tagtgagtga tgatcatagc
     6061 accaaaacct tcaccacgca tggcattgac acctttagac acaactttaa gagcgtcaat
     6121 atcaagacct gagtcaatct cgtccaaaag agcaaatgtt ggctccaaca tcaaaagttg
     6181 aagaatttca ttgcgttttt tctcaccacc agagaagcct tcgttgaggt aacgctctgc
     6241 catttcttct ttcatgttga gcaattccat tttttcatct agcttagtaa taaactcacg
     6301 aactgaaatc ttctcatcat cttctttacc cgcattcata gcggcacgaa gaaactcagc
     6361 attggtaatt ccagggattt ctgatgggta ttgcatagca aggaaaagtc ccatacgcgc
     6421 acgctcatcc acttccaact caaggatgtt tacgccatca aacaaaactt cacctttagt
     6481 tacttcatag tttggatttc ccatgatagc ggcagaaaga gtcgatttac ctgtaccatt
     6541 tggtcccatg atagcggcaa tttctcctgt tttcagggtc aggttaaccc cttttaaaat
     6601 ttcttttcct tcaatctcaa cgtgaagatc tttaatctct aatactgaca tgatagttcc
     6661 tttctttttc aaaactttta cagtacttac tagaaaaacg tctgatttcc ccagaaatag
     6721 agcaaaaatt ctataaatat acttttctag tataacaaaa aaaagcctaa aaggctttga
     6781 ttttgtctgg aagctgaggg ctagtctttc ctattaaaat gctgatcgat gacatcaacg
     6841 attttgccca tcacataact gactcggaaa tttctaaaga gtaaaattgc ccaaaaggct
     6901 gccataatat agcggccagt catccaggct tcattgaaaa aataggtctc agcagctgta
     6961 aacagcgcca taataataaa ttgttgttta ttcataaact tattgtatca tgaaatcttt
     7021 ctttagtctt cctctacctt cactttatca gccaaaaatt caaatccttc tggaatcagg
     7081 ctttcttcta cttctttttc agtttgagaa gatttggctt tggctgaaaa ggctgcgcga
     7141 acttctttcc attcctccat ggaaatagct aaaatctcag gtgaaaaacc tgccgcctga
     7201 ctgaggatat taccaaacat ggtattgaga ttgtctcgtt tcatagtttg accagcattg
     7261 aagttagact caaaagcaag aatagcatgg tgttcattgg cagcaaccgg ttgagaacca
     7321 actagcagag ccttgtccgg cccacctaga ctttcaatta cctctcccca ggcattctgc
     7381 aaacgaatta gattttgacg tgctaaatca ggattttcga cggcctcttg taagatagat
     7441 tgcactttat tgcgatcgac acgatagact gttttgcccg tagctggtcg actaggagct
     7501 ggtgcaactt gtttaggaac cgcacctgca ttagaaagct cttgtttgag acgggcaact
     7561 tcctgtctca gcgtagcaat ttcattttca accgctcctg atagagctgg ttcgggcttg
     7621 atttccgcca aacggacggt catcatttca gcataaatct tgggctgcaa actagactta
     7681 atatctgcta aattcactgt tgctaagcga atcatttcaa acagattttt ttgaggaagt
     7741 gccaaatttt ctacaaagac tgaactatga tgagtatttt ctccccctgt ttgaacaatt
     7801 aacaagtctc ttaaatagtg caaaagatcg gtcacaaaac gagtcatgct cttaccattg
     7861 tcaaaaagaa gattcaagca agacaaagct ttgggaacat cctgttgaga caaggccgcc
     7921 acataatcat ccaaggctga taggctaatg gtgccagtaa tttcttcaga gatagcagtc
     7981 gtcagctcat ttccctgtgt caaactcagg gcttgatcca aaatagacaa ggcgtcccgc
     8041 attccacctt ccgcccgtct ggcaatgatt tccacagcct ctggttcaga actgatattt
     8101 tctttttcta agatatagtg aatatgttcc ttaatatcct gtgtcttaat tgatttaaac
     8161 tcaaaacgtt gcacacggga tagaatagta gcaggaatct tgtgcaattc agtagtggcc
     8221 aaaataaaga ctacattctg tgttggttct tccagcgtct ttaggagggc attaaaagcc
     8281 cctgtagaca gcatgtgaac ctcatctatg atataaacct tataacgagc aaggctaggc
     8341 gcataggtag atttatcacg aatttcgcga atttcatcta ccccattatt agaagctgca
     8401 tccatttcaa tgacatcttc taaactaccg tccgtcactg cttgacaaat atagcagtta
     8461 ttgcaaggtt cgccacccac ttgattggga cagttcatag ccttggcaaa gattttagca
     8521 acactggttt ttcccgttcc acgaggacca gaaaaaagat aagcgtgact tattttctct
     8581 tgctccaccg cttgtttaag agtcttagcc acaacttctt gaccaactaa ctgggagaag
     8641 ttttgacttc tatattttcg ataaagtgct tgatacatta agatttttcc tcaaacatcg
     8701 taaagtccca tgttgtcttt tcaagcaaaa tagcgacaaa ttgttccaaa taatctcgat
     8761 ccatagcatc gtaatcctca atctctgaag aatccagatc cagaactcca agtaactgac
     8821 cattcttcat catcggcacc acaatttcac ttttagctag actatcacaa gaaatatagt
     8881 tgagataggt cgtcacatct ccaacaataa cagtttcctg aaagtgagct gcctcaccac
     8941 aaacaccctt gcctagtgca atacggatgc aggaaacacc tccttggaag gggcctaaaa
     9001 ccaattcctt tccatcgaac aaataaaagc ctgcaaatac ggtattagga aaacgtgatt
     9061 ttatgagagc actggcgttg gaaagattag ccaaaacatt ggtttcgcct tccaatagga
     9121 aggacaattc ttcatttaac atttgataac gtgattgttt ttctgatttt aacatagaac
     9181 tattatatca aaaaaggagc agagacaaaa gaaaaatccc ttgtttagta aacaaaggat
     9241 tttgggctct ataatatttg tagtgggtaa cccccctata gatattatgg agcctatttt
     9301 attgtagaaa aaaagtccca taagatctat aatgaaaagc gac
//
LOCUS       DAVGJL010000083         9249 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.083, whole genome shotgun sequence.
ACCESSION   DAVGJL010000083 DAVGJL010000000
VERSION     DAVGJL010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9249
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.083"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            342..929
                     /locus_tag="VVE42_001514"
     CDS             342..929
                     /locus_tag="VVE42_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073693.1"
                     /translation="MFVFADDSLSANSKVVSGEAQFENGSSVRFGDTQVNILSDEVLE
                     VVNPDGSVDTIERRADGVYINGAFYMAYQKNEIDLNISFRSYDPNVWNYVNTIHGNKQ
                     ANTFANFMTGAGISYMIGRIGALLGGPWGAIIGGAYFGIQAYQSYLDSQSPYPYYITS
                     TYIHVAQRKWKFITEYYRNSNYTGYVKTVTTYVNF"
     gene            complement(1016..1162)
                     /locus_tag="VVE42_001515"
     CDS             complement(1016..1162)
                     /locus_tag="VVE42_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004247184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073694.1"
                     /translation="MLVEFHKSQGTLETPEAQAEIAQKREEIEQRRAELEDKKQELLN
                     RLNK"
     gene            complement(1410..2114)
                     /locus_tag="VVE42_001516"
     CDS             complement(1410..2114)
                     /locus_tag="VVE42_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEU3073695.1"
                     /translation="MMSELFGEFLGTLILILLGNGVVAGVVLPKTKSNSAGWIVITMG
                     WGIAVAVAVFVSGKLSPAHLNPAVTVGVALKGDLHWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEVEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
                     IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPIPNKGDGDWSYAWIPVV
                     GPVIGAALAVLVFSLF"
     gene            complement(2182..4008)
                     /gene="glpO"
                     /locus_tag="VVE42_001517"
     CDS             complement(2182..4008)
                     /gene="glpO"
                     /locus_tag="VVE42_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEU3073696.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKSDP
                     MLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTPTANKVLSKDQVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKGTQFSQMRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     SAVSKLESSTSEKYLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSRGLDSKEAHYLANLYG
                     SNAPKVFALAHSLEQAPGLSLADTLSLHYAMRNELALSPVDFLLRRTNHMLFMRDSLD
                     SIVEPVLDEMGRFYDWTEEEKATYRADVKAALAQNDLAELKN"
     gene            complement(4050..5558)
                     /gene="glpK"
                     /locus_tag="VVE42_001518"
     CDS             complement(4050..5558)
                     /gene="glpK"
                     /locus_tag="VVE42_001518"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEU3073697.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEV
                     RSNSEIYGKTAPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYA
                     RDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQVDTQTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEVDD"
     gene            complement(5717..7144)
                     /locus_tag="VVE42_001519"
     CDS             complement(5717..7144)
                     /locus_tag="VVE42_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU3073698.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKALDSGLELTIHSEDENLRLSIGAATKGRDIRSLFLESAVKYQILVYLFY
                     HQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIRYFYFCLF
                     RKVWSSQEWEGHMQKPERKQEIANLEEICGASLSVGQKLDLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPAFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QTPVYYLKNDLDMEDLVAIRQLLFT"
     gene            7309..8181
                     /gene="hslO"
                     /locus_tag="VVE42_001520"
     CDS             7309..8181
                     /gene="hslO"
                     /locus_tag="VVE42_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEU3073699.1"
                     /translation="MDKIIKTISESGAFRAFVLDSTETVRTAQEKHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGTGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKVAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGAEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            8168..9148
                     /gene="dusB"
                     /locus_tag="VVE42_001521"
     CDS             8168..9148
                     /gene="dusB"
                     /locus_tag="VVE42_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEU3073700.1"
                     /translation="MTNLNTPFMIGNVEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLIN
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALLQLEKV"
BASE COUNT         2646 a         2113 c         1831 g         2659 t
ORIGIN      
        1 ttatttttta ttgtagagca gacccaagcg aatgaacata ggttgtaaaa agcaaatgaa
       61 aaaactgcga ggataatagc ttctgtgtct aaataaccca tttcgagacc tcttttcaac
      121 aacaacaagc ctgtagtttc gataacgatc aaacataaaa atatataaaa aattttcgca
      181 ttaagcatat tgttaccgct ttctttttat ttggttttat tgtataataa aagttgaaaa
      241 aaatcaaaaa tacaaaggag tttccgttat gtctaagatg tttaaatgca tgtgtttgct
      301 agttatgtct gttttctgta tgtctagcat ttccccgtcg cttgtttgtt tttgctgatg
      361 atagcttatc ggcaaactct aaagttgtat ctggtgaggc tcagtttgag aatggctcat
      421 cagtgcggtt cggagatacg caagtaaata ttttaagcga tgaagttctt gaggttgtca
      481 atccagatgg aagtgtagac actattgaac gacgtgctga cggcgtctat atcaatgggg
      541 cattttatat ggcttatcaa aagaatgaaa tcgacctaaa tatatctttt agatcatacg
      601 atcctaatgt ttggaattac gtcaacacaa tacatgggaa taaacaagct aacacattcg
      661 ctaacttcat gactggtgct ggaatcagtt atatgattgg taggataggt gctttattgg
      721 ggggtccatg gggtgcaata attggtggag catattttgg tatacaagct tatcagtctt
      781 acttggattc acaaagccca tatccatact acataacaag tacttatatc catgttgctc
      841 aaagaaaatg gaagtttatc actgaatact atagaaactc aaactatact gggtatgtaa
      901 aaaccgtaac aacctacgtg aatttctaaa atataaaccc tgcctagata taaaggcagg
      961 gtttatattt tagttaattg ctcaagtaat ttgtaagcat aatacttgcg aaactctatt
     1021 tgttcaagcg gttcaaaagc tcttgttttt tatcctcaag ctcagctctc ctttgctcga
     1081 tttcttcacg tttttgggcg atttctgctt gcgcctcggg agtttccaaa gtgccttgtg
     1141 acttgtgaaa ttctactagc atatcaacga tttttgggtc taattcgttg acataatcag
     1201 gatttgacta tttcggcacg ttgcttgcag gtgcttgctt tttgccgcct tttttgggcg
     1261 ttttggcttg ttgttcagct ttctgactgt agttgacctc gtggaatttt cgtgcttgat
     1321 atgatgcgtt ttattatgag aagatttact tcattttttg ctgaaattgg gctcttgccc
     1381 aagctctatc aacataattg ttttctagat tagaaaagtg agaatacaag cactgccaag
     1441 gctgctccga taacagggcc tacaacagga atccaagcgt aagaccagtc tccgtctccc
     1501 ttgtttggaa ttggcaagat gctgtgcatg atacgaggtc caaggtcacg agctgggttc
     1561 aaggcataac ctgttgtccc acctagtgat agaccgatac cgacaatcaa agttcccact
     1621 gcaaaggttc cgatacctgc ctgaaaatcg taaagaccca aagcaaagat tgtcaacacc
     1681 aaaacaaagg ttccaaggat ttcgctaatc aagtttgata cagtatcctt gatggctggt
     1741 ccagtactga aggttgccag gatattgcct gcattttctt ctacctcata atgcggtttg
     1801 aattgcaacc aaaccaaaat ctgacccagc atggcccctg cgaactgggc taagatataa
     1861 ggcaaaacgg aagcccaatg caagtcacct tttaaagcta caccaactgt cacagctgga
     1921 tttaaatgag ctgggctgag ttttccagat acaaatactg caaccgcaac tgcaatcccc
     1981 caacccatag taatcacaat ccaacctgca ctgttgctct tggtttttgg aagaaccaca
     2041 cctgcaacaa caccatttcc tagaagaatc aggattaaag tccctaaaaa ttctccaaat
     2101 aattcactca tcatttttct gtctccatta aaaaaggggc gggcgacaag gattgctacc
     2161 ctccacctct tttatttttt cttaattttt taattctgct aagtcatttt gagcaagagc
     2221 tgcttttaca tcagcacggt aagttgcttt ttcttcttct gtccagtcat agaatcgtcc
     2281 catttcatcc aaaactggct caacgatact atccaagcta tcacgcataa agagcatgtg
     2341 attggtacga cgaagaagga agtcaactgg gctaagagcc aactcgttgc gcattgcata
     2401 gtgaagggac aaagtatctg ccaagctgag tcctggcgct tgttccaagc tgtgagcaag
     2461 tgcaaagact ttcggtgcat ttgaaccgta aagatttgcc agatagtgag cttccttgct
     2521 atccaaacca cgtgatactc caagttgcgc aaaggcttcg atttctgaat ccacatttgc
     2581 tgggttcaat tctccacctg aaacagggta agttttagaa ttgatcaatt taaagctacg
     2641 gtcaaattct gctttgagga tgtcaaccac gcgctccata gctccttcag ccatcttacg
     2701 gtagtctgtg attttaccgc cagcaagagt caaaagacca ttgtcatcac ggtccaagct
     2761 agacccacga gaaactgcag atggatccaa atatttctca gatgtgctac tttcaagctt
     2821 gctgacagca gactcaacat cttcacgtgt tttttctttg gagagataag attcaacagt
     2881 cgcaatcaag ttgtcaaagc tttcatcact gatggtaccg ttatttccac cattatagtc
     2941 agaggcactg ttccctgcaa tcaatggacg aagacctgcc cagctgcttt cgatatcatc
     3001 aatggtgatg ttggattctg gaaagcggtt gttgacaatg ccaagtagat aatctacatc
     3061 ttcttgagtt acttttggat gctccaaatc acctgtgtag tctgtatcag ttgtaccaaa
     3121 gtaagtcttg ttttcacgtg ggagaacaaa gaccatacga ccgtcaccca aacctgtgtc
     3181 aaagtaaact ggctgtgaaa ccttgatttt gcttgaatct actaccaagt gaactccctt
     3241 agttgggcgc atttgtgaga attgcgttcc cttattagac aaattacgta ctttatcact
     3301 ccaaggacct gttgtattaa taaccagacg ggccttgatt tcaaatactt ggtctgtcaa
     3361 gagatcacga gctacaacac ctgtaatctt gccactttcg tcaaaaagga agccttctgc
     3421 cttcacgtgg ttggcaatga gggcaccgtc ttggttggca cgtttgatgt tttcaatcac
     3481 gagacgcgca tcgttgttac ggaagtcaag atacactcca cctcctacca agccttcctt
     3541 cttcaagttt ggctggcgtt ccaagacttg atccttgctc aaaaccttgt tcgcagttgg
     3601 tgtgttgcta acacctgcca agaggtcgta caagtccatg gctactttaa gacggaagag
     3661 gctaaaggtt gctccatctt catcgtaaac tggtaagagc attggatctg attttggaat
     3721 gtgtggagcg atttgttgaa ccactgcacg ttcagaaacc gtatctgaga ccacttctac
     3781 gtcgaattgt ttgaggtaac gaagtcctcc gtgaaccaat tttgttgaac gactagatgt
     3841 tccttctgca aagtcttgca tttcaatcaa accagtctca agaccgctag ctgccgcctg
     3901 caaggctaca ccagctcctg tgattcctcc accgataatc aagaggtcca gggtacgttc
     3961 ctgcattttt ttaattgaca attcacgtgt tttctttgaa aattccatat ttacacactt
     4021 tctaaataaa tcgctttatt ctgccagtat tagtcgtcta cttccgcaaa gacttgagtt
     4081 gctttcacag ccttcttcca gcccttgtag agttgttcct tgcgagattc gttcatagat
     4141 ggctcaaaga gttctcctgt ctcgttcaag agtttcaact cgtccaagtc tttccagtac
     4201 cctactgaca aacctgctag gaaggccgct cctagagctg ttgtttccag gtttttagca
     4261 cgtgcaatgt caatgcctaa aatatccgcc tggaactgca tgaggaagtt gttcatggct
     4321 gcaccaccat ccaccttcag tacttgaatg gcggtctgag tatccacttg catggtgtcg
     4381 atgatatcac gcacttgata agcaatagat tgcaaagtcg ccttgataaa gtcttctttg
     4441 cttgttccac gagtcaaacc aaagacggaa ccacgagcat tttggttcca gtatggagcg
     4501 cctagacctg taaaggctgg aacgacataa acttcatcgt tgttgtgaga atcacgagcg
     4561 tatttttcag attctggtga attttcaacc atgcgaagac cgtcacgaag ccactgaata
     4621 gcacttcctg cgatgaagat agaaccttcc aaggcataat aaaccttacc gttgattccg
     4681 taaccaatgg ttgtcaagag gttgttttca gacaactgca tctcttcccc agtattcatg
     4741 atgatgaaag agcctgttcc ataagtattc ttaaccatac ctggctcaaa agccaactgt
     4801 ccaaagaggg ctgcttgttg gtccccagcc atacctgaga ttggcacctc tccaccgtag
     4861 aaatggaatg gagctgtctt gccgtagatt tcggagttag aacgaacttc tggaagtata
     4921 gccttcggaa tgttaaggat ttccaaaatc tcatcatccc atttgagttc tttaatgtta
     4981 taaagcatgg tacgagctgc atttgagtag tcagtcacgt gagccgcacc gtcagtcaat
     5041 ttccaaacca accaagtatc gatagtacca aagagcaatt cccctttttc tgctcgctct
     5101 tgagcacctt ctacatgatc caaaatccaa cgaaccttgg tagcagagaa gtaagcatca
     5161 ataatcaaac cagtcttttc atggaatttt tccacataac cttggctttt tagttgctca
     5221 gccaaaggtg ctgtctggcg tgactgccaa acgatagcat tgtagatagg aagtcctgtt
     5281 ttcttatccc agacaaccgt tgtttcacgt tggttggtaa tcccgattgc ctcgatttga
     5341 tttggcttga caccactttc gatgaaagca cccgcaataa ctgactgaac agagttccaa
     5401 atttcattgg cattgtgctc aacccaacct gcttgaggga aaatctgggt aaactctttt
     5461 tgactcgagc taaccttttc cccttttttg ttgaaaatga tggcacgaga acttgtagtt
     5521 ccctggtcaa tggccatgat gtatttttct tgtgacatgt actgtcctcc tgataaagat
     5581 cttgaggtgt ttcctcttta cacttactag tatacaaaat aaagcgcttt cttgtttatc
     5641 aacttttttt aatttttaaa aaaatgtgag gagattgtgt gaatttcaca aaaaagacct
     5701 ggattaacca agccttttaa gtgaataata actggcgaat cgctaccaaa tcctccatat
     5761 ccaagtcatt ttttaaataa tagactggtg tctgttcctt cttatgaatc gggttattgg
     5821 taaccagcaa atcataatgc cgactagggt cataagcttc aatggtaata aaacgattgt
     5881 attccaaata ccgtttcaaa atggctgcca tccttgaaaa gacaagaaaa ccagatgtca
     5941 aatccagacc aatccgcata tgctgaccac cgttttcagc catatattcg attaactgga
     6001 gccattccca gagaatcttc ttatctaaat ctgtcccctg ttgaaaagca ggtagagcat
     6061 gaaaaatctc ctctgctgtc tctttaaaat catgttccat cagtaaatat ggatgacgat
     6121 tctctaactg gtacttgtag cgatcctgta aaatatagcc cttatagaga tagacttgag
     6181 cacaaagctg actgagttca taggtgacat ggtcctctgt ataatccccc aatagttcct
     6241 ccttcttcat ttcttgaatc aattgcgtca gtaaatctgc caactgccct ccaaaaccaa
     6301 gaatatactc catagtatga agaggaagaa tgcgatgaga taggagaaaa gagaagaata
     6361 tcatcatctc accatcctca actcctagtg gaatatgttg cccagcaaaa aaggacggaa
     6421 cctttctcag caaacgaaga tagaagatat tgtcaaaata ccctcgcatt ttctcttcta
     6481 tgacttgaaa ctgacaagca ttgacccgaa gacgttgttg actgatgtga gcccagagaa
     6541 ccaagtccaa tttctgcccc acagacaaac ttgcaccgca gatttcctct aaattggcaa
     6601 tctcctgttt tctctctggt ttctgcatgt gaccttccca ttcctgactc gaccagacct
     6661 ttcggaaaag acagaaatag aaatagcgaa tctgatgctc tggacctcgc caacgtccat
     6721 tttggatgga taaatcaaat tctgacaaaa tctgatttaa accagccaag tgacgaccaa
     6781 gcgtagcctc gctaatcacc aattcttgag ccagctgatg ggctaaaaac tgttggtggt
     6841 agaaaagata aaccaaaatc tggtatttaa cagcactctc caaaaacaag ctccgaatat
     6901 ctctcccctt ggtagctgca ccgatagaca gacgcagatt ttcatcttct gagtgaatag
     6961 tcagttctaa gccactatcc aaagccttgt cattgagcag ggtgacatat ttggttaggg
     7021 ttgcttttga aaatcctgtt tcttccatta cagccttgac ggtcgtctga gactcttgta
     7081 atagaaagga aaggattgaa aattgaccac actcggcttt ctccatcaaa tctcctaaat
     7141 acatttgtta cccctttcat tttctacctt aagcatagca taaatcttac aaatgctaaa
     7201 ataatctgtt tctcttttta ttttcatgct gatttcctgg tccattatcc tgaaaatcag
     7261 taaacacacg gctccccctt tgggcatttt tatgctaaaa tagtagctat ggataaaatt
     7321 attaaaacta tatcagaaag cggagccttt cgtgcttttg tccttgatag cacggaaacc
     7381 gtccgcactg ctcaagaaaa acatcaaacc caagctagct caactgtagc gcttggtcga
     7441 actcttatcg ccagccagat tctcgcagcc aatgaaaaag gaaataccaa acttacagtt
     7501 aaggtgttgg gctctagctc tctaggtgct attatcaccg tcgctgatac caaggggaac
     7561 gtcaaaggct atgttcaaaa tcctggtgtt gacatcaaaa agactgcgac tggtgaagtc
     7621 ctagtcggac cttttgttgg aaatggtcaa ttcctcgtta tcacagacta cggtactgga
     7681 aatccttaca actctataac tcccctcatc tctggagaaa tcggtgaaga ccttgccttt
     7741 taccttactg aaagccaaca aacgccttca gcggtcggcc tcaatgtcct tttggacgag
     7801 gaagacaagg tcaaggttgc aggtggtttc ctagttcaag tcttgccagg agccaagaaa
     7861 gaagagattg ctcgctttga aaaacgcatc caagaaatgc cagctatctc tactcttctc
     7921 gaaagcgacg accatatcga agccctcctc aaggctatct acggggacga agcctacaag
     7981 cgtctttctg aagaagaaat ccgtttccaa tgtgactgta gccatgaacg ctttatgaac
     8041 gctcttgcca gccttccaag ctcagactta caggaaatga aagaggaaga ccacggggca
     8101 gaaatcactt gtcaattctg ccaaactact tacaactttg atgaaaagga cctggaggaa
     8161 ctcattcgtg acaaatctta atacaccttt tatgattggc aatgttgaga ttcccaatcg
     8221 taccgtttta gcgcctatgg ctggcgtgac caactcagcc tttcgtacca tcgcaaaaga
     8281 gctcggagct ggactcgttg taatggaaat ggtctctgac aagggaatcc aatacaacaa
     8341 cgaaaaaacc ctgcacatgc ttcatatcga tgagggcgaa aaccctgtct ctatccaact
     8401 ttttggtagc gatgaagaca gcctagcacg cgcagcagaa ttcatccaag aaaacaccaa
     8461 gaccgatatc gtcgatatca acatgggctg ccctgtcaac aaaatcgtga agaacgaagc
     8521 tggtgctatg tggctcaagg atcctgacaa gatctactct atcatcaaca aggtccagtc
     8581 tgtccttgat atcccactta ctgtcaaaat gcgtaccggc tgggcggacc catctcttgc
     8641 agtagaaaat gccctcgctg ctgaggctgc aggtgtttct gccctcgcca tgcatggccg
     8701 tacccgtgaa caaatgtata ctggccacgc agaccttgag accctttaca aggttgccca
     8761 agctctaacc aagattccat tcatcgccaa cggtgatatc cgtactgtcc aagaagccaa
     8821 gcaacgcatc gaagaagttg gtgctgacgc agtcatgatt ggccgagctg ccatgggaaa
     8881 tccttacctc ttcaaccaaa tcaaccatta ctttgaaaca ggagaaatcc tacctgattt
     8941 gacctttgaa gacaagatga agatcgccta cgaacacttg aaacgattga ttaacctcaa
     9001 aggagaaaac gtcgcagttc gtgaattccg cggcctcgct cctcactatc tccggggaac
     9061 atctggcgct gccaaactcc gtggagccat ttcgcaagct agcaccctgg cagagattga
     9121 agccctcttg caattggaaa aagtataatg caaaaaagag cctaggacaa aagtataata
     9181 ttaaatataa aaagcgaaca aaatcagtta tctgacactc agaattctgt cttgttcgct
     9241 tttttgtct
//
LOCUS       DAVGJL010000084         9167 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.084, whole genome shotgun sequence.
ACCESSION   DAVGJL010000084 DAVGJL010000000
VERSION     DAVGJL010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9167
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.084"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            1..1515
                     /gene="rlrA"
                     /locus_tag="VVE42_001522"
     CDS             1..1515
                     /gene="rlrA"
                     /locus_tag="VVE42_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus transcriptional regulator RlrA"
                     /protein_id="HEU3073701.1"
                     /translation="MEKRITDKITILNILLDIRSIELDELSTLTSLQSKSLLSILQEL
                     QETFEEELTFNLDTQQVQLIEHHSHQTNYYFHQLYNQSTILKILRFFLLQGNQSFNEF
                     TQKEYISIATGYRVRQKCGLLLRSVGLDLVKNQVVGPEYRIRFLIALLQFHFGIEIYD
                     LNDGSMDWVTHMIVQSNSQLSHELLEITPDEYVHFSILVALTWKRREFPLEFPESKEF
                     EKLKNLFMYPILMEHCQTYLEPHANMTFTQEELDYIFLVYCSANSSFSKDKWNQEKKT
                     HTIQLILQHTRGKHLLSKFKNILGNDISNSLSFLTALTFLTRTFLFGLQNLVPYYNYY
                     EHYGIESDKPLYHISKAIVQEWMTEQKIEGVIDQHRLYLFSLYLTETIFSSLPAIPIF
                     IILNNQADVNLIKSIILRNFTDKVASVTGYNILISPPPSEEHLTEPLIIITTKEYLPY
                     VKKQYPKGKHHFLTIALDLHVSQQRLIYQTIVDIRKEAFDKRVAMIAKKAHYLL"
     gene            1780..3042
                     /locus_tag="VVE42_001523"
     CDS             1780..3042
                     /locus_tag="VVE42_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEU3073702.1"
                     /translation="MEHINHTTLLIGIKDKNITLNKAIQHDTHIEVFATLDYHPPKCK
                     HCKGKQIKYDFQKPSKIPFIEIGGFPSLIHLKKRRFQCKSCRKVTVAETTLVQKNCQI
                     SEMVRQKIAQLLLNREALTHIASKLAISTSTSTVYRKLKQFHFQEDYTTLPEILSWDE
                     FSYQKGKLAFIAQDFNTKKIMTILDNRRQTTIRNHFFKYSKEARKKVKVVTVDMSGSY
                     IPLIKKLFPNAKIVLDRFHIVQHMSRALNQTRINIMKQFDDKSLEYRALKYYWKFILK
                     DSRKLSLKPFYARTFRETLTPRECLKKIFTLVPELKDYYDLYQLLLFHLQEKNTDQFW
                     GLIQDTLPHLNRTFKTTLSTFIRYKNYITNAIELPYSNAKLEATNKLIKDIKRNAFGF
                     RNFENFKKRIFIALNIKKERTKFVLSRA"
     gene            complement(3100..5424)
                     /gene="pflB"
                     /locus_tag="VVE42_001524"
     CDS             complement(3100..5424)
                     /gene="pflB"
                     /locus_tag="VVE42_001524"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEU3073703.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIDPIRDEVLEFESVKANFEKSLDWLTDTYVDALNIIHYMTDKYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
     gene            5819..6880
                     /gene="dinB"
                     /locus_tag="VVE42_001525"
     CDS             5819..6880
                     /gene="dinB"
                     /locus_tag="VVE42_001525"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEU3073704.1"
                     /translation="MLIFPLLNDLSRKIIHIDMDAFFAAVEIKDNPKLRGKPVIIGSD
                     PRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKAVGLQIRAI
                     FKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGVSYNKF
                     LAKMASDYQKPHGLTVILPEQAEDFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLE
                     VPEVTLIDRFGRLGYDLYRKARGIHNSPVKSNRIRKSIGKEKTYGKILRAEEDIKKEL
                     TLLSEKVALNLHQQEKAGKIVILKIRYEDFSTLTKRKSLAQKTQDASQISQIALQLYE
                     ELSEKERGVRLLGITMTGF"
     gene            complement(7027..7872)
                     /locus_tag="VVE42_001526"
     CDS             complement(7027..7872)
                     /locus_tag="VVE42_001526"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEU3073705.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLIATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGVAFIYLEKRNKARAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
     gene            complement(7903..8040)
                     /locus_tag="VVE42_001527"
     CDS             complement(7903..8040)
                     /locus_tag="VVE42_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073706.1"
                     /translation="MRYQTHYQLKQKLWLEPFGNRQAIGGNELIHSGLFQAYHLGFGS
                     S"
     gene            8127..9059
                     /locus_tag="VVE42_001528"
                     /pseudo
     CDS             8127..9059
                     /locus_tag="VVE42_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT         2881 a         1878 c         1707 g         2701 t
ORIGIN      
        1 attgaaaaaa gaattacaga taaaattacc attttaaata tattattgga tattcggtct
       61 attgaactag atgaactgtc tactttaact tcattacaat ctaaaagtct cctaagcatt
      121 ttgcaggaat tacaagaaac attcgaggaa gagttgacat ttaatttaga tacgcaacaa
      181 gttcaattaa tcgaacacca ttctcatcaa acgaattatt acttccatca gctttataac
      241 caatcaacta ttttaaaaat actccgcttc tttctactac aaggtaatca aagctttaac
      301 gaatttacac aaaaagagta tatttccatc gcaacaggct accgcgtacg acaaaagtgt
      361 ggcttgttac tacgttcagt aggtttggac cttgtgaaaa atcaagtagt tggtcctgaa
      421 tatcgcattc gttttttaat tgcactcctc cagtttcatt ttggtattga gatttatgat
      481 ttgaatgatg gaagtatgga ttgggtcaca catatgattg tgcagtctaa ttctcaactc
      541 agtcatgaac tgttagaaat cacacctgac gagtatgttc attttagcat actagtggca
      601 ctcacttgga aacgtcggga atttccactt gagttccctg agtccaaaga gtttgaaaaa
      661 ttaaaaaacc tctttatgta tccgattttg atggaacatt gccaaactta cttagagcca
      721 catgccaaca tgacttttac tcaggaagaa ctggattata tctttttggt ttattgttct
      781 gctaattctt cgttttccaa ggacaaatgg aatcaagaaa aaaagactca tactattcaa
      841 ctgattcttc agcatacaag aggaaagcat ctactatcca agttcaagaa tatcttagga
      901 aatgacattt cgaattctct ttctttttta acagcactga ctttcttaac aagaacgttt
      961 ttgtttggtt tacaaaatct agttccctat tataattatt acgaacatta tgggattgaa
     1021 agcgacaagc cactgtatca tatttcgaaa gcaattgttc aagagtggat gactgagcag
     1081 aagattgaag gagttatcga ccagcatcgg ctgtaccttt tctcactcta cttgacagaa
     1141 accatctttt cctcactacc tgctatcccg atttttatca ttttaaataa tcaagcagat
     1201 gtaaacttaa taaagtccat tattttacgg aattttactg acaaagttgc ctctgttaca
     1261 ggatacaata ttctaattag tcctcctcct tcagaggagc atttgacaga gccattgatt
     1321 attattacaa cgaaagaata cctgccttac gttaaaaagc agtatcccaa agggaaacat
     1381 cactttttga cgattgcctt agatttgcat gttagccaac agcgtctcat ctatcagaca
     1441 attgtagata tccgaaaaga ggcttttgac aaacgggtcg caatgattgc taaaaaggct
     1501 cactatttgt tataagttca tcgtactgtc tacacacaaa aaggggcttc ctaagccatc
     1561 tctattctta tccccatatt tcccagtttt gggtaaatct gaacagaaaa acacttctgc
     1621 tctctagttt ctgctagtga acaaaagtgt tttttctggc tctataattt ctgtagtggg
     1681 taaatcccac tgtggagatt atagggcttt ttgagtatca aaaaagtccc ataagattta
     1741 taatgaaaag cgcctaaaca actcattaga aagtatctta tggaacacat caatcatacc
     1801 acattactca tcggaataaa agacaaaaac atcaccttaa ataaagccat tcagcacgat
     1861 acccatatcg aagtcttcgc tacacttgat tatcacccac ctaaatgtaa acactgtaaa
     1921 ggaaaacaaa tcaaatacga cttccaaaag ccttctaaaa tcccttttat cgagattggt
     1981 ggtttcccta gcctcattca tttgaaaaag agacgatttc aatgcaagtc ctgtcggaaa
     2041 gtcactgtag ctgaaacaac tctcgttcag aaaaattgcc aaatctcgga aatggtgaga
     2101 cagaaaattg cccaactcct actcaacaga gaggctctta cacatatcgc ttctaaatta
     2161 gccatctcta cctctacctc taccgtctat cgtaagctca agcaatttca tttccaagag
     2221 gattacacca ctttgcctga aatcctctcc tgggatgaat tctcctacca gaaggggaaa
     2281 ttggctttca ttgctcagga tttcaacact aagaaaatca tgaccattct tgataacaga
     2341 cgtcaaacaa ccatccgaaa tcatttcttc aagtactcga aagaagctag aaaaaaagtt
     2401 aaagtcgtca ctgttgatat gtctggaagt tatatccctc tcattaagaa attatttccc
     2461 aatgctaaaa ttgttctcga tcgtttccac attgtccaac acatgagcag ggctcttaat
     2521 cagactagaa tcaacatcat gaagcaattt gatgataaat ctctggaata cagagctctt
     2581 aaatattact ggaaatttat cctaaaagat agtcggaaac tctctcttaa gcctttctat
     2641 gctagaactt tcagagagac cttaactcct agggagtgtc tgaagaaaat ctttactcta
     2701 gtacctgaac ttaaagatta ctatgacctg tatcaactac tcctatttca tctacaagag
     2761 aagaatactg accagttttg gggcttaatt caagacacgt tacctcatct caaccgcacc
     2821 tttaaaacca ctttgagcac atttattcgc tataaaaact acatcactaa cgccattgaa
     2881 ttgccttatt ctaacgctaa acttgaggcc actaacaaac tcatcaaaga catcaagcgc
     2941 aatgcctttg gttttcggaa ctttgaaaac ttcaaaaaac ggattttcat cgctctgaac
     3001 atcaaaaaag aaaggacaaa atttgtcctt tctcgagctt agcttttctt caacccacta
     3061 cagttgacaa agagcccttt ttattattca agaacttgat tagctcaatg catccaaggc
     3121 gtcatccatt gaaagaactt cgtggaagac acgttgtgtc aattcagttt tttgttctgg
     3181 agtgaggtat ttagtgttta cacagtatcc agagatacgt acgataacgt cttcgcctga
     3241 catgattttt tcgtaaacat cgttcaagtc cataacgttc aagttaacgt gttgtccacc
     3301 gttttcgaag taaccatcaa ggattgttac caagttatca acttgttcat cacgagtctt
     3361 accaagagcg cgaggtgata cttgtgtagt caatgagata ccgtcagctg cataactaaa
     3421 gtcaaggcta gaaagtgagt tcaagttttg caaccaacca cctttagctt tgttagatgg
     3481 gttagcacct ggtgagaaga attcaagttt agacaagttc acagaaccat cttcgttgag
     3541 gtatacacct ttgtgaactg gtgagttacc agtttgttta gagtaagcaa cgttagatgt
     3601 gattgtcaaa agtgatactg tagcttctgc gtctttgtat agtttgtggc tacgtagacg
     3661 agttgtgtaa gcttcgatca accattctgc caattcgttt gaacgtgggt catcttcacc
     3721 ccagcgtggg tagtcaccga ttgtttcgta atcgtagatg tagccatctt catcacggat
     3781 tggtttaact gtagcgtatt taatcgctga caatgtatca acagtgttag caaatccaca
     3841 aataccgaat cccatgttgg cacgttgttt agttggcaag aaggccattt gaacagcttc
     3901 gtagttgtac ttatcagtca tgtagtggat gatgttcaag gcatctacgt aagtgtcagt
     3961 caaccagtca agagattttt caaagttcgc tttaactgat tcaaattcaa gaacttcgtc
     4021 acgaatagga tcgatgtcaa atactttgta gtctttgtga acatcgtcgt aaccaccatt
     4081 caaaccagta agaagggctt taagaacgtt tacacgagca ccgaagtact ggatgttgtg
     4141 gcgttgttct tcattttctg gatcaagtgg agacacacag catgagatac agctcatttc
     4201 accatatccg tctttagcca ttgttgttac accttcgtat tggatagaag agtgtttgtg
     4261 gctcatgtgc atacagtagc gacggaagtt gtatggcaat ttgtcagtcc aaagaactgt
     4321 caagtttggc tctggagagt taccgatgtt gtcaagagtg ttcaagaaac ggtagtccat
     4381 cttagtaaca cggtgacgac cgtcgttacc cataccagcc atagaagttg tgatgaatgt
     4441 tgggtcacct gagtacaatt ggtcataagc ttttgtacga gcaaatttaa ctgtacgaag
     4501 tttcataacg aaatcatcaa cgaattcttg gatttctgat tcagtaaatg taccacgagc
     4561 aaggtcacgt tctgcaaaga tgtccaatac gattggtaca cgacctagag atgtagcagc
     4621 accgttaatc acacggcaga cagccatgaa agcaatgtta acccattgga ttgcttcttt
     4681 cacgttcatc gctggtttgc gaacatcaac cccgtaaagg tcacccaggc gaacaacttg
     4741 ttgcaatgct tggtattgaa ggtttacttc ttcacgaaga cggattgttt cttcatcgat
     4801 ttctttgatt gcattccagt cgtttacttt ttcttgcatc aagtagtctg caccgtaaag
     4861 agcaagacgt gcgtaaacac cgatgatacg tccgcgtgag tatgcatctg gaagaccagt
     4921 tacagtgtgt gcatgacgag cgcgacgaat atttgaagtg taggcacgga aaataccgtc
     4981 gttaactgtt gttacatatt tagtgaagat ttcgtgaaca gctgggtctg gttcgtatcc
     5041 attttctttt aaagtagttt cagccatacg gataccacct tttggcatga agttcaattt
     5101 gaagagttca tcattttgga taccaaagat aacttcattt tctttgtcga taaatccagc
     5161 agggatatca gcgatagatg ttggacgagt gtccattggg aaacgagttt cttcgtagtg
     5221 tgctttagtt tcttctacaa ttttcttgat gtgaagtgaa cgctctgttg gtcctgcaag
     5281 gaagctttcg tctccatcat aaggtgtgta gttagcttgt acaaagcgtg atacacttgc
     5341 tttttctttc caatctacgc ctttgaagcc ttcccaagct ttgtcaaaaa tatcttgtgc
     5401 ttcaacaact gtcttaacaa ccatattaat gtcctctttt ttctagtaac agtcatctgt
     5461 tacattcatg agacaagtat accataaagt aaccgatttc aacaagtgaa aaattcctat
     5521 ttttacactt tcttttctaa aacagtctat attttatttc aaactgtatt atatttttga
     5581 aaaaaataaa gtcctttttt ctttttttca gaaaaaaggg tataataaaa gaaaataagc
     5641 agtaacactc aatggaaatc gaaaagcaaa ctaggaagct agccgcaggc tgctcaaagc
     5701 actgctttga ggttgtaaat agaactgacg aagtcagctc aaaatactgt tttgaggttg
     5761 cagatggaag ctgacgtggt ttgaagagat tttcgaagag tataaaaagg tgctaggcat
     5821 gttgattttt cctttgttaa atgatttgtc aagaaaaatc atccatattg acatggatgc
     5881 cttttttgct gcggtggaaa tcaaggataa tcctaaactc agaggaaaac ctgtcattat
     5941 tggaagcgac cctcggcaaa caggtggacg gggagtcgtt tctacctgta gctatgaggc
     6001 tcgagctttt ggtgtccatt ctgccatgag ttccaaggaa gcttatgaac gttgtcccca
     6061 ggccgtcttt atctcaggaa attatgagaa atacaaagct gtgggactcc agattcgagc
     6121 tatttttaag cgctatacag atttgattga acccatgagc attgacgaag cctatttgga
     6181 tgtgacagaa aataaactcg gtatcaagtc agcggtcaaa attgctcgcc tcattcaaaa
     6241 agatatctgg caagaactcc atctaactgc ttccgcaggc gtttcttaca acaaattctt
     6301 agctaaaatg gcgagtgatt atcaaaaacc acatggtttg acagtgattc tacctgaaca
     6361 ggctgaggat tttctcaaac aaatggatat ttccaaattt catggagtag gaaaaaagac
     6421 agtagaacgt cttcatcaaa tgggcgtttt tactggtgct gatttacttg aagttcctga
     6481 ggtaacccta atagaccgtt ttggtagact aggctatgat ctatatcgaa aggctcgtgg
     6541 cattcacaac tctccagtca aatccaatcg catccgtaaa tcaatcggca aggagaaaac
     6601 ctacgggaag attctccgtg ccgaggaaga tatcaaaaaa gagctgactc ttctatcaga
     6661 aaaagtcgct ctcaatctac atcaacaaga aaaagctgga aaaattgtca ttttgaaaat
     6721 ccgctacgag gacttttcaa ctcttaccaa acgaaaaagt cttgctcaaa aaacacaaga
     6781 tgctagtcag ataagccaaa tagccctgca actctatgaa gaattaagcg agaaagaaag
     6841 aggtgtccgc ctattgggga ttaccatgac tggattttaa agctcaatga aaatcaaaga
     6901 gcaaactagg aagctagccg caggctgctc aaaacactgt tttgaggttg cagataaagc
     6961 tgacgcggtt tgaagagatt ttctaagagt ataaaacctt gaagagttgc cccttcaagg
     7021 tttttcttat acaaataaac ggacaaaact gtaaagtagc aaaacactac caagcacgat
     7081 acggtattta ccaaaaaggg tgaagtcgtg ttttttcaca tagctggtca agaagcgaat
     7141 agccaccatg ctgaccgcaa aagctactcc catcgcgacc aagagcaaaa acaattgccc
     7201 aaagctcaag agttctccgg ctttcacaaa tttgaaaatc tttaaggcac tagctccaaa
     7261 cataacggga atcccaagat agaaggtaaa ttctgtcaca actgaacgac tggttccatt
     7321 taacaaacca ccgacaatcg ttgcacctga acggctagtc cctggtaaaa gagcaagaac
     7381 ttggaagagt ccgatataga aagcggtcgt ataaggaagc ttgtccaact ctgttacact
     7441 tggctcgata gcacgcgctt tattgcgctt ttccaaatag atgaaggcaa ccccgtagat
     7501 aatcaacatg agagcaactg aaaccatgtt atggaagtgg gtatcaaacc aatcatcaaa
     7561 tttaaagaca ccaagtaaag gtaaagtagc aatcaagacc ttcaaccata gtctccaagt
     7621 cttacgaact tcctgtttgt ccttagtcgg tttaaaagga ttgagcttgt taaaataaat
     7681 caccataact gctaaaatag caccaagctg aatcacgaca ttaaacatgg acataaaggc
     7741 ttcattttga ttttggtatt ggatgaattc ctctgctaaa atcaagtgac ctgtactgga
     7801 aatcggcaac cattccgtaa ttccttcaac aatcccgaag aagatagatt ttaaaatttc
     7861 aataagatac atagattact ccctatatta tatttagcgc agttagctac tgccaaagcc
     7921 caagtggtat gcttggaata agccactgtg gattagttca tttcctccaa tcgcttgacg
     7981 atttccgaaa ggctctaacc acagcttttg tttcaattgg taatgagttt gataacgcat
     8041 agacgtttta tctcgtctaa ggtaacaagg caaaaagttc gtggataacc aagtagactt
     8101 gaaatactat atctggaggt actcccatgc tttatgtggg cattgatgtc gctaaaaata
     8161 aacacgatgt tacagccttg aatgttccag gaaaactgtt cttaaaccac tcactttttc
     8221 aaataataaa gctggttttg aactcttaga tctgtctctt cgacagctca accaagactg
     8281 tctcatcgct cttgaagata ctggacatta tgcctttaat ctactgaact tcctgcatga
     8341 acaaggatat aaagtttaca cctacactcc tttgctcatc aaggaatttg ccaaatcatt
     8401 atcacttcgc aagactaaaa cggataagaa agacgctcat ggcatcgctc ttaaacttct
     8461 ttctgacccc aatcgtgaac aatttcaaca cgataatcgg caagtagaac taaaaatact
     8521 gactagacat attcatcgtc tcaagaaaaa acagtctgat tggaaagtac aatacactcg
     8581 ttgtcttgat atcatctttc ctgagttgga taaaatcgtt ggaaagcatt cagaatatac
     8641 ctaccaactc ttgacacgct accctaatcc tcagaaaagg cttgaggcag gatttgataa
     8701 gctgatagaa attaagcgat tgaccgcttc taaaattcag gatatcctct cagtcgcacc
     8761 tcgttctatc ggaacaacat ctcctgctcg tgaattcgaa atcatcgaaa tcatcaaaca
     8821 ttacaagagg ctcattgaca aggcggaaac atgtgtcaat gacttgatgg ctgagttcaa
     8881 ctcggtcatc acgacggtca ctgggattgg gaatcgttta ggggcggtca ttttagccga
     8941 gatttgataa tcctgctcaa ttacaagctt tcgctggact ggattcttct atttatcagt
     9001 caggtcagat tgatttagct ggaagaatgg tcaaacgggg ttcccctcat ctgcggtagg
     9061 tactcataca agctgccaaa gcatgccctc gcttttcacc tgcttttaag gcctatctta
     9121 agactaagtt agaacaagga aaacattaca atgtagccat catccac
//
LOCUS       DAVGJL010000085         8914 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.086, whole genome shotgun sequence.
ACCESSION   DAVGJL010000085 DAVGJL010000000
VERSION     DAVGJL010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8914
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.086"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            120..275
                     /locus_tag="VVE42_001529"
                     /pseudo
     CDS             120..275
                     /locus_tag="VVE42_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
     gene            475..3750
                     /gene="rexB"
                     /locus_tag="VVE42_001530"
     CDS             475..3750
                     /gene="rexB"
                     /locus_tag="VVE42_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEU3073707.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLEYLSQQASFSITVTRFAQMARYLVLNDLPAKTTLDDIGLGLAFYKCLAELDPKDL
                     RVYGAIKQDPQLIQQLIELYHEMTKSQMSFLDLENLTDEDKRADLLLIFEKVTAYLNQ
                     GQLAQGSQLSHLIEAIENDKVSSDFNQIALVIDGFTRFSAEEERVVDLLHGKGVEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKASRLLE
                     SSYDFSELALDVDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYRFRQEDLINLL
                     RTDLYTDLSQSDIDAFEQYIRYLGINGLPAFQQTFTKSHHGKFNLERLNVLRLRILAP
                     LETLFASRKQKAEKLLQKWSVFLKEGAVTKQLQDLTTTLEAVEQERQAEVWKAFCHVL
                     EQFATVFAGSQVSLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKISQNTSLLTDEERQNLNQATEEGVQLLIASSENLKKNRYTMLSLVNSA
                     RKQLFLSAPSLFNESESKESAYLQELIHFGFRRREKRMNHKGLSKEDMGSYHSLLSSL
                     VAYHQQGEMSDTEQDLTFVKVLSRVIGKKLDQQGLENPAIPTSPSSKTLAKDTLQALY
                     PAKQEFYLSTSGLTEFYRNEYSYFLRYVLGLQEELRLRPDARSHGNFLHRIFERALQL
                     SNEDSFDQRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPAIE
                     TIKEEANFGGKDQAFIQLDNGRSVFVRGKVDRIDRLKANGSIGVVDYKSSLTQFQFPH
                     FFNGLNSQLPTYLAALKREGEQNFFGAMYLEMAEPVQSLMAVKSLAGAVVEASKSMKY
                     QGLFLEKENSYLGEFYNKNKANQLTDEEFQLLLDYNAYLYKKAAEKILAGRFAINPYT
                     ENGRSIAPYVQQHQAITGFEANYHLGQARFLKKLDLADGKRLVGEKLKQAWFEKIREE
                     LNR"
     gene            3747..7397
                     /gene="addA"
                     /locus_tag="VVE42_001531"
     CDS             3747..7397
                     /gene="addA"
                     /locus_tag="VVE42_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEU3073708.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSGQNIL
                     VSASAGSGKTFVMAERILNQLARGVEISQLFISTFTVKAATELKERLEKKISKKIQET
                     DDVDLKQHLGRQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLLLE
                     NEVFHEVFEAHYQGKQKETFSHLLKNFAGRGKDERGLRQQVYKIYDFLQSTSNPQKWL
                     SESFLKGFEKADFTSEKEKLTEQIKQALWDLESFFRYHLDNDAKEFAKAAYLENVQLI
                     LDEIGSLNQESDSQAYQAVLARVVAISKEKNGRALTNASRKADLKPLADAYNEERKTQ
                     FAKLGQLSDQITILDYQERYHQDTWELAKTFQTFMSHFVEAYRQRKRQENAFEFADIS
                     HYTIEILENFPQVRESYQERFHEVMVDEYQDTNHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAQNPQEGKLILLKENFRSSSEVLSATNDVFERLMDQEVG
                     EINYDNKHQLVFANTKLTPNPDNKAEFLLYDKDDTGEEEESQTETKLTGEMRLVIKEI
                     LKLHQEKGVAFKEIALLTSSRSRNDQILLALSEYGIPVKTDGEQNNYLQSLEVQVMLD
                     TLRVIHNPLQDYALVALMKSPMFGFDEDELARLSLQKAEDKVHENLYEKLVNAQKMAS
                     SQKGLIHTALAEKLKQFMDILASWRLYAKTHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIRMIDQVLEAQHDLASVAVAPPKDAVELMTIH
                     KSKGLEFPYVFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLESKEYPAAK
                     NGKLNSNTRLQARNFQDWLWVISKVFTKDKLNFSYRFIGEDQLTREDIGELETKSPLQ
                     DSSQADNRQSDTIKEALEMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVGKKISFDLPDFSTKEKVTGAEIGSATHELMQIIDLSQQLTLASLTETLK
                     QVQTSQAVRDKINLDKILAFFDTALGQEILANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIENIEKYLI
                     LLGKDEVQVVKV"
     gene            7612..8790
                     /locus_tag="VVE42_001532"
     CDS             7612..8790
                     /locus_tag="VVE42_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073709.1"
                     /translation="MTLPVRKSLHDAVLQASKADTWEQATKEWNEVSLIFNGIGRSNC
                     VCGNAIKYAYELFNGVTGQRLFPIGSDCVRHFHRLSLDQQLEEEEKLLRKVENLTRKA
                     QKKEKIKVNKSDFDERLLKWLWEKGVFKPNRGNQFAPERDYQLFLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERAIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWLLNSYFRELPEEKARFSRLLLEYRKNGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDHRSSAQGSWIEGILLNCLKPFLERLVI
                     "
BASE COUNT         2762 a         1863 c         1890 g         2399 t
ORIGIN      
        1 gattgaaata agatacgaac agcttgatta gggaaatcaa attaatttct agaaatattt
       61 tagcatccac ggtgtactgt tatagtttca atttactata gttcaatcat tctatcaaga
      121 atattgaata tataaaggca cgtaatatta aaacttcttc tgatatcgaa ttagagtccg
      181 atgttgatgg cgataaatca gacaatctac ccgtcgacat taagatactg ggacaacaca
      241 ttgaagtgta ttccgtacct aagaaacaat cataaaactc aaagccttgt taccgaggct
      301 tttttattat gtgattttca gtagccagac ttattctatc taatacaagt aaagctaagt
      361 atttaagatt tagcagatga tgaagaaaag aaaggatggc ctaaatattt taacagattt
      421 acgaggctaa tcatggtata atatagggtg atgaaaaatt ccaacgaggc tgagatgaaa
      481 ttactttata ctgatattcg gacttctttg acagaaattc taacaagaga ggcagaagag
      541 ctagttgcag ctggcaagcg ggtcttctac attgccccca actctctttc ttttgaaaag
      601 gaacgcgccg tgctggaata cttgtcccag caggcttctt tttcgattac cgtcacgcgc
      661 tttgctcaaa tggctcgcta tctggtcttg aatgatttac cagctaaaac tactcttgat
      721 gatatcggtc ttgggttggc cttttacaaa tgccttgccg aactcgatcc caaggacttg
      781 cgtgtttatg gcgctattaa gcaggatcct caattgatcc agcagttaat tgagctttac
      841 catgagatga ccaaatctca gatgagtttt ttggacttgg agaatttaac agatgaggat
      901 aagagggcgg atttactctt gatttttgag aaagtaacag cctatcttaa tcaaggtcag
      961 ttagcccagg gaagtcagtt gtcccatttg attgaggcta ttgagaatga caaggtaagt
     1021 agtgatttta atcaaatcgc cttggtcatt gacggcttta ctcgtttttc tgctgaggaa
     1081 gagcgggttg tggacttact tcacggcaaa ggtgttgaga ttgttatcgg ggcttatgct
     1141 agtaagaaag cctataccag tccttttagc gagggcaatc tctaccaagc cagcgtaaaa
     1201 tttctccatc atctggcttc taaataccaa acgcctgctc aggactgttc tcaaactcat
     1261 gagaagatgg atagttttga caaggcctct cgtttgttgg agtcttctta tgacttttca
     1321 gaactcgctt tggatgtcga tgagaaagac cgtgaaaatt tacaaatctg gtcttgtttg
     1381 acgcaaaagg aggagttgga gctagtagcc cgtagtattc gtcagaaatt acatgagaac
     1441 tcagacctga gctacaagca ttttcgtatt ctcttggggg atgtagcttc ttaccagtta
     1501 tctctcaaaa ccatttttga ccagtatcag attccttttt atcttggtag aagcgaagcc
     1561 atggctcatc atcccttgac tcagtttgtc gagtctattt tagctttaaa acgttaccgt
     1621 tttcgtcagg aggatttgat taatcttctt agaactgatt tgtatactga cctcagtcag
     1681 tctgatattg atgcttttga gcaatatatc cgctatcttg gtatcaatgg cttgccagcc
     1741 tttcagcaaa ccttcaccaa atcccaccat ggaaaattta atcttgagcg tttgaatgtc
     1801 ctccgcctga gaattttagc acctcttgaa accctctttg ccagccgaaa acaaaaggct
     1861 gaaaaactcc tacaaaaatg gagtgtcttt ctaaaagaag gagctgtgac caagcagtta
     1921 caagatttga caaccacttt ggaagctgta gaacaggaaa gacaagccga agtttggaag
     1981 gctttctgcc atgttttaga acaatttgcg actgtttttg ctggttcaca ggttagtctg
     2041 gaagacttcc tagccttgct ccattctgga atgagtttgt cccaataccg taccattcca
     2101 gcaacagtgg acactgttct ggtgcagagt tacgatttga ttgcaccatt gactgctgac
     2161 tttgtctatg ctattggact aactcaggac aatttaccaa aaatttctca aaacaccagt
     2221 cttctgacag atgaagaaag gcaaaatcta aaccaagcga ccgaagaagg cgttcaatta
     2281 ctgattgcca gcagtgaaaa tctcaagaaa aatcgctaca ctatgctttc cttggtcaat
     2341 tctgctcgta agcagttgtt cttgtcggct ccaagccttt ttaacgaaag tgaaagtaag
     2401 gaatctgcct atcttcaaga gttgatccat tttggattta ggcggagaga gaagaggatg
     2461 aatcacaaag gactgtctaa ggaggatatg gggtcctatc acagtctttt gtctagtctg
     2521 gttgcctatc accagcaggg tgagatgagc gatactgagc aagatttgac ttttgtcaag
     2581 gttctgtcgc gtgtcatagg taaaaaacta gatcagcaag gtctggaaaa tccagctatc
     2641 ccaaccagtc caagcagcaa gaccttagcc aaggacacct tgcaagctct ctatcctgcc
     2701 aaacaggagt tttacctgtc tacgtcgggt ttgacagagt tttatcgcaa tgaatacagt
     2761 tatttcctac gctacgtttt aggcttgcag gaggaattac gtttgcgtcc tgatgcccgt
     2821 agtcacggga atttcttgca tcgtatcttt gaacgcgcct tgcagttgtc taatgaagat
     2881 tcctttgacc aacgtctaga acaagctatt caagaaacca gtcaagaacg cgaatttgaa
     2941 gctatttatc aagaaagttt ggaagcccag tttaccaagg aagttttgct tgatgttgca
     3001 cggacaactg gacatattct ccgacacaat ccagccatcg aaaccatcaa agaagaagca
     3061 aattttggtg gaaaagacca agcctttatt caattagaca atggacgcag tgtctttgta
     3121 cgaggcaagg tggaccggat tgaccgtttg aaagctaatg gatcgatagg agtagtagac
     3181 tacaaatcca gtctgactca gttccagttt cctcatttct ttaatgggct caattctcag
     3241 ttaccaacct atcttgctgc cctaaaaaga gaaggggagc agaacttttt cggcgccatg
     3301 tacttggaaa tggctgaacc tgtccaatct ctgatggcgg taaaaagtct ggcaggagca
     3361 gtggtagaag ccagcaaatc tatgaaatac caagggctct tcttggaaaa agaaaacagt
     3421 tatttaggcg aattttataa caaaaacaag gctaatcaac tgacagatga ggaatttcag
     3481 ctcctactgg actacaatgc ctatctttac aagaaagctg ctgagaagat tttagcaggc
     3541 cggttcgcca tcaatcctta tactgaaaat ggcagaagca ttgccccata cgtccagcaa
     3601 catcaggcta ttacaggctt tgaagccaat taccatctgg gccaagcccg tttcctaaaa
     3661 aagttggacc tagctgatgg caagcgtctg gtcggagaaa aactcaagca agcttggttt
     3721 gaaaaaataa gagaggagtt aaatcgatga agcttattcc ctttttaagt gaggaggaga
     3781 ttcaaaaact gcaagaagca gaagcaaatt cgagcaagga acaaaagaaa actgccgagc
     3841 aaatcgaagc tatctacact tctggccaga atatcctggt ctcagcatcg gctggttctg
     3901 gaaagacctt tgtcatggca gagcgcattc tgaaccaatt ggcgcgtggt gtcgaaattt
     3961 ctcaactctt tatctcaacc tttaccgtca aggctgcaac tgaacttaaa gaacgtttag
     4021 agaaaaaaat cagcaagaaa attcaagaaa cagatgatgt cgacctcaaa caacacttgg
     4081 gacgccagtt ggcagaccta ccaaacgctg ccattggaac catggactct ttcacacaaa
     4141 aattccttgg caaacatggt tatctgcttg atattgcacc taatttccgt attttacaaa
     4201 accaaagcga gcaacttctt ctcgaaaacg aagtctttca tgaggtcttt gaagcgcatt
     4261 accaaggtaa acagaaagag acctttagtc atttgctgaa aaactttgct gggcgtggca
     4321 aggacgaacg gggtctgcgc cagcaggtct ataaaatcta tgacttcctc caatccacca
     4381 gtaatcctca aaagtggttg agtgaatctt tcctcaaagg ctttgagaaa gctgatttta
     4441 ccagtgaaaa agaaaaactg actgagcaaa tcaaacaagc cctttgggat ttggaaagct
     4501 ttttccgtta ccatctggat aacgatgcca aggagtttgc aaaggctgcc tatttagaaa
     4561 atgttcagtt aattctggat gaaattggct ccctaaatca ggagtccgat agtcaggctt
     4621 atcaggcagt gcttgcgcgt gttgtcgcca tctctaagga gaaaaacggt cgagctctga
     4681 ctaatgccag tcgtaaggct gatttgaagc ccctggctga tgcctacaac gaagagagaa
     4741 agacccagtt tgctaaacta ggacaattat cagaccagat aaccattctc gactatcaag
     4801 aacgttatca tcaagacaca tgggaactag ctaaaacctt ccaaaccttt atgagtcatt
     4861 ttgtggaggc ttatcgtcag cgcaaacgcc aggaaaatgc ctttgaattc gctgatatca
     4921 gccattacac cattgagatt ttagagaatt tcccacaagt tcgtgagtct tatcaggagc
     4981 gcttccatga agtcatggtc gatgagtatc aggataccaa ccatattcaa gaacggatgc
     5041 tggaattgtt gtctaatggc cacaatcgct ttatggtggg agatatcaag caatccatct
     5101 atcgtttccg tcaggcagac ccgcagattt tcaatgagaa attccaacgc tatgcgcaaa
     5161 atccccaaga aggcaagctc attctcctca aggaaaattt ccgtagtagt tcagaagtgc
     5221 tgtcagcaac caatgatgtc tttgaacgtc tcatggacca agaggtcggc gaaatcaact
     5281 atgataacaa gcaccagctt gtttttgcca ataccaaact gactcccaat ccagacaaca
     5341 aggcagaatt tctcctctac gacaaggacg atacaggtga ggaagaagag agtcaaacag
     5401 aaacgaaact aacaggcgaa atgcgcttag ttatcaagga gattctgaaa cttcatcaag
     5461 aaaaaggtgt tgcctttaag gaaattgccc ttctgacctc cagccgcagt cgtaatgacc
     5521 agattctcct cgccctgtct gagtacggga ttcctgtcaa aactgacgga gagcaaaaca
     5581 attatctcca atccctagaa gtgcaagtca tgctagacac tcttcgtgtc attcacaatc
     5641 ccctgcaaga ctacgccttg gttgccctta tgaagtctcc aatgtttggt tttgatgagg
     5701 atgagctagc acgtttgtcc cttcagaaag cagaggataa agtccacgaa aatctctatg
     5761 agaaactggt caatgcacaa aaaatggcaa gtagtcaaaa aggcttgatt cacacagctc
     5821 tagctgaaaa actaaagcaa ttcatggata tcctagcttc ttggcgcttg tatgccaaaa
     5881 cccactctct ctatgacttg atttggaaga tttacaacga ccgtttttat tatgactatg
     5941 ttggggcttt gccgaatggt cctgctaggc aggccaatct ctatgcccta gcactgcgtg
     6001 ctgatcaatt tgaaaagagc aatttcaaag gtttgtcgcg ttttattcgt atgattgacc
     6061 aagtcttaga agcccagcac gatttggcaa gcgtggccgt cgcaccgcca aaagatgcag
     6121 tagagctcat gaccatccac aagagtaaag ggctggagtt tccttacgtc tttatcctca
     6181 atatggatca agatttcaac aagcaagact ctatgtcaga agtcattctc agtcgtcaga
     6241 atggtcttgg tgtcaaatat attgccaaga tggagacagg ggcagtagaa gaccactatc
     6301 ctaaaaccat caaactctcc attcctagtc tgacctatag gcagaacgaa gaggaattac
     6361 agctagcaag ctattctgag cagatgcgtt tgctgtatgt tgctatgacg cgggctgaga
     6421 aaaagctcta tcttgtcggc aagggttctc gtgaaaagct ggaatccaag gaatacccag
     6481 cagccaaaaa tgggaaacta aatagcaata ctagactgca agcacggaat ttccaagatt
     6541 ggctttgggt tatcagtaaa gtgtttacta aggacaagct caactttagt tatcgtttta
     6601 ttggcgaaga tcagttgacc agagaagata tcggagagtt ggaaaccaag agtcctctcc
     6661 aagatagctc ccaagcagac aatcgtcagt cagataccat caaagaagct ctggaaatgc
     6721 tgaaggaggt ggaagtttat aatactcttc accgcgcagc tattgaactt cctagtgttc
     6781 aaaccccaag tcaaatcaag aaattctacg aaccagttat ggatatggaa ggtgtcgaga
     6841 ttgctggtca aggtcagtca gtaggcaaga aaatcagctt cgatttgcca gatttttcaa
     6901 ccaaagaaaa ggtaactgga gctgagattg gtagtgctac tcacgaactc atgcagataa
     6961 ttgacctcag ccagcaacta acccttgcta gcctaacaga aacactcaaa caagttcaaa
     7021 ctagccaagc tgtcagagac aagatcaatc ttgataaaat tcttgctttc tttgacacag
     7081 cactcggtca ggaaattctt gctaataccg accatctcta tcgcgagcaa cctttctcca
     7141 tgctcaaacg agaccaaaag agtcaggaag actttgttgt ccgtggtatc cttgatggct
     7201 atctgcttta cgaaaacaaa attgttctgt tcgactacaa gacagaccgc tatgatgaac
     7261 caagtcaact cgtagaccgc tatcgtggtc agttagctct atacgaagag gctttatcac
     7321 gagcctattc gattgaaaat attgaaaaat acttgatttt actcggtaaa gacgaggttc
     7381 aagttgtaaa agtataactt atactcttcg aaaatctctt caaaccacgt cagcttcgcc
     7441 ttgctgtata tatggttact gacttcgtca gttctatcca caacctcaaa acagtgtttt
     7501 gagctgactt cgtcagttct atctacaacc tcaaaacagt gttttgagct gactgcggct
     7561 agctttctag tttgctcttt gatttttatt gagtattaga aaggagacct catgacactt
     7621 ccagttagaa aatccctgca cgatgcagtt ttacaggctt cgaaagcgga tacttgggaa
     7681 caagctacca aggaatggaa tgaagtttcc ttgattttta acggtattgg ccgtagcaat
     7741 tgtgtctgtg gaaatgccat caaatacgct tatgaactct ttaacggtgt cacaggccaa
     7801 cgcctctttc caattggtag cgactgtgtt cgtcattttc atcgattgtc ccttgaccag
     7861 caattggaag aggaagaaaa actgctcaga aaggttgaaa atctaaccag aaaagctcag
     7921 aaaaaggaaa aaatcaaggt caataaaagt gactttgacg agcgacttct aaaatggctc
     7981 tgggaaaaag gtgttttcaa acccaatcgt ggcaatcaat ttgcgcctga gagagactac
     8041 cagcttttcc tagaagtctt tcagggagga agttggacca aggcggaacc aaagaaaaag
     8101 gctcggatgg aagaagtcct tgaaaagtgt atcaaaccct ttttacttgg gaaatcggat
     8161 gaccaactct accttgtcaa gctaggcaag gagaaaattg actacgaaca agaactccgt
     8221 atccaggcag agaaagaacg taagaagagg gataaaatcg ccaagcaata tgcagacaat
     8281 ctggttctag ccatgggacc cgcagagcgt gcttatcaag attactttgg ctttacagag
     8341 accctgactc aagaagaacg caagtgggaa aaaattcttt ttggtaaaaa tagagcagaa
     8401 cgggctatca aggccaaaca ataccaaaaa gagctggaaa aagaccaacg aattgccagt
     8461 caggatccaa ttgaaagaaa acagaagcag acttggctcc tcaattccta ttttcgtgag
     8521 cttcctgaag aaaaagctcg attttctagg ctcttattag aatatcgaaa aaatggtgaa
     8581 gtaccctttt caactgaata cctgtcagat catctcatcg actttttcta taaaatgaaa
     8641 gcctttgaat ttgaaatcgc accagaacaa gtccgagatt ttctaaaaaa aagcctgcag
     8701 gaagaccatc gctcatcagc acaaggaagc tggatagaag gaattctcct caactgcctc
     8761 aaaccatttt tagaacgatt agtgatatga gtaacaccta ccgtgtaaat ttcatggtag
     8821 gtgttattgt acccccaaat aatataaaac aaagtatgaa agcgcatctc aattttaaga
     8881 cttttttgta aattaagagt ataattaagg tata
//
LOCUS       DAVGJL010000086         8878 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.087, whole genome shotgun sequence.
ACCESSION   DAVGJL010000086 DAVGJL010000000
VERSION     DAVGJL010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8878
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.087"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            82..969
                     /locus_tag="VVE42_001533"
     CDS             82..969
                     /locus_tag="VVE42_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000909058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HEU3073710.1"
                     /translation="MLISPLMTPILGVGLSLAIFDFKLLRKSFKILAIQILASLIAST
                     LYFYLSPISYASSEIVARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATAL
                     MPPLCTVGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDS
                     KMRRILLLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGHNILKKTYSKKTHT
                     LKLTISGNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEYFFQYIKD
                     KEAKEKEKANKFYTESEEQ"
     gene            1136..3223
                     /gene="topA"
                     /locus_tag="VVE42_001534"
     CDS             1136..3223
                     /gene="topA"
                     /locus_tag="VVE42_001534"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEU3073711.1"
                     /translation="MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNDEVKEVLSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEAASFITDRFGS
                     KYSKHGSKVKSASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQKGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEEKIK"
     gene            3329..3688
                     /locus_tag="VVE42_001535"
     CDS             3329..3688
                     /locus_tag="VVE42_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEU3073712.1"
                     /translation="MRLIYLIIGFLSLALAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQAYVADFRETKSITRERKKKIIVSIYVLMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
     gene            3781..4413
                     /locus_tag="VVE42_001536"
     CDS             3781..4413
                     /locus_tag="VVE42_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEU3073713.1"
                     /translation="MIYEFCAENVTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYNDLEIAIMLEDIRLTAQAGSQGVVFGALTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQPEAIDWLSQAGVTRILTRAGVSGDSL
                     EKRFVHYHRILEYAKGKIEILPGGGIDLENRQTFIDQVGVTQLHGTKVVF"
     gene            4457..5113
                     /locus_tag="VVE42_001537"
     CDS             4457..5113
                     /locus_tag="VVE42_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEU3073714.1"
                     /translation="MFEIFKSYQFNQEKAHDYGFIENSEVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETGDLYPHVYMESMRGSFVGNVREACLEILYQIRKACFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMKISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELIEKSWNLTSKK
                     "
     gene            5132..5764
                     /locus_tag="VVE42_001538"
     CDS             5132..5764
                     /locus_tag="VVE42_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEU3073715.1"
                     /translation="MNFQLAKYSLLKKFLENIGFTRLEEYGAIFKYLIENVKTDRQII
                     CSPHCHDDLGMAVANSLAAVKNGAGRVEGTINGIRERAENAALEEIAVALNIRQDYYQ
                     VETSIVLNETINTSEMVSRFSGIPVPKNKAVVGGNTFSHESGIHQDGVLKNPLTYEII
                     TPELVGVKIPLGELSGRHAFVEKLRELALDFTEEDIKPLFAKFKALADKK"
     gene            5792..6226
                     /locus_tag="VVE42_001539"
     CDS             5792..6226
                     /locus_tag="VVE42_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HEU3073716.1"
                     /translation="MVAGTMVENPEGFHFDDLQLQTHADNDIEALVSLANMDGEKVEF
                     NATGQGSVEAIFNAIDKFFNQSVRLVSYTINAVTDGIDAQDRVLVTVENRDTETIFNA
                     AGLDFDVLKASAIAYINANTFVQKENAGEMGRSVSYHDMPSV"
     gene            6238..7275
                     /gene="leuB"
                     /locus_tag="VVE42_001540"
     CDS             6238..7275
                     /gene="leuB"
                     /locus_tag="VVE42_001540"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEU3073717.1"
                     /translation="MAKKIVALVGDGIGPEIMEAGLEVLEALAEKTGFDYEIDRRPFG
                     GADIDAAGPPLPDETLKASREADAILLAAIGSPQYDGAAVRPEQGLMALRKELNLYAN
                     IRPVKIFDSLKYLSPLKPERISGVDFVVVRELTGEIYFGDHILEERKARDINDYSYEE
                     VERIIRKAFEIARNRRKIVTSIDKQNVLATSKLWRKVAEEVAQDFPDVTLEHQLVDSA
                     AMLMITNPAKFDVIVTENLFGDILSDESSVLSGTLEVMPSASHSENGPSLYEPIHGSA
                     PDIAGQGIANPISMILSVSMMLRDSFGRYEDAERIKRAVETSLAAGILTRDIGGQAST
                     KEMTEAIIARL"
     gene            7272..7541
                     /locus_tag="VVE42_001541"
     CDS             7272..7541
                     /locus_tag="VVE42_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEU3073718.1"
                     /translation="MKLDEKITLVLLIWNVIIFLIYGIDKSKARRRVWRIPEKILLIL
                     AFTFGGFGAWLAGIIFHHKTRKWYFKIVWFLGMVTTLVALYFIWR"
     gene            7545..7904
                     /locus_tag="VVE42_001542"
     CDS             7545..7904
                     /locus_tag="VVE42_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEU3073719.1"
                     /translation="MAGSSREYAAWALADYGFKVVIAGSFGDIHYNNELNNGLLPIVQ
                     PREVREKLAQLKPTDQVTVDLEQQKIISPVEEFTFEIDSGWKHKLLNSLDDIGITLQY
                     EELIAAYEKQRPAYWQD"
     gene            8122..8265
                     /locus_tag="VVE42_001543"
     CDS             8122..8265
                     /locus_tag="VVE42_001543"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073720.1"
                     /translation="MTSDKAGLERKFAAKERKRNKPGVVLCGSMDELCALAQLNPEIE
                     AFY"
     gene            8517..8687
                     /locus_tag="VVE42_001544"
     CDS             8517..8687
                     /locus_tag="VVE42_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073721.1"
                     /translation="MVSMVDKDGKLIPEQGGARSTSPAPVVIRKGLDIDKIMMHLSDT
                     FNSWDYRQVEYY"
BASE COUNT         2715 a         1584 c         1986 g         2593 t
ORIGIN      
        1 gatttagcga ttttactatg tgccatgctt atcgcctcta tcggattaaa tatggattcg
       61 actcccgtga ttattggagc catgttaatc tctcctttga tgacacctat tctgggagtg
      121 gggctctctc tagctatatt tgattttaaa ttgttaagaa aatcttttaa aatattagct
      181 attcaaattc ttgccagttt aatagcttca acactttatt tttatctttc tcccatttcg
      241 tatgctagtt cggaaattgt tgctagaacc tctccgacta tttgggatgt tctcattgct
      301 tttgtaggag ggatagcagg tattattggt gctaggaaaa aagagaccaa taatattgtt
      361 cctggtgttg ctattgcaac cgccttgatg cctcctcttt gtacagtagg ttatgctatt
      421 gcttctgcta atctaaaatt tatcataggc tcctcttacc tattcctcat caattgtagc
      481 tttattgtca ttgcgactta tataggtgtt aggttgatga tggttaagaa acattatttt
      541 aaagataatg aagaagactc taaaatgcgt aggattttgc ttctagttgc tgttttgctg
      601 atgattccga gtttcatctc tgcaacgact ttagtgagag aaacgttgaa aaaagagtcc
      661 cttaagaaat ttatatcaga gcagtttcag gggcataata ttttgaaaaa aacctattct
      721 aaaaagactc ataccctaaa gctaaccatt tcaggaaatt atttgacaga agaagaactc
      781 gatatgattt caagtaagag aggtgactat ggtttaagtg atgtttctgt tcaagtttca
      841 caattgtctg attcagaaca acttagtaag gaagaactgg tggagtattt cttccagtat
      901 atcaaggata aggaagcaaa agaaaaggaa aaagctaata agttttatac agagtctgag
      961 gagcaataat ttcttgagaa tagctggttt ttctcgtgag tcttctatgt atatcaaagg
     1021 aagactgagg ttttaagtat aaaacttttc ttctattata gtagctaagt cttgacaggg
     1081 ttcaaaaaat ggtttacact ttataaagtt tattactttg aaaaggtgtg atactgtggc
     1141 tacggcaaca aaaaagaaaa aatcaacagt taaaaaaaat ctagtcatcg tggagtcgcc
     1201 tgctaaggcc aagacgattg aaaaatatct aggcagaaac tacaaggttt tagccagtgt
     1261 cgggcatatc cgtgatttga agaaatccag tatgtccgtc gatattgaaa ataattatga
     1321 accgcaatat atcaatatcc gaggaaaagg ccctcttatc aatgacttga aaaaagaagc
     1381 taaaaaagct aataaagttt ttctcgcgag tgacccggac cgtgaaggag aagcgatttc
     1441 ttggcatttg gcccatattc tcaacttgga tgaaaatgat gccaaccgtg tggtcttcaa
     1501 tgaaatcacc aaggatgcag tcaaaaatgc ttttaaagaa cctcgtaaga tcgatatgga
     1561 cttggtcgat gcccaacaag ctcgtcggat cttggatcgc ttggtagggt attcgatttc
     1621 gcctattttg tggaagaagg tcaaaaaggg cttgtcagca ggtcgcgttc agtccattgc
     1681 ccttaaactc atcattgacc gtgaaaatga aatcaatgcc ttccagccag aagaatactg
     1741 gacagttgat gctgtcttta aaaagggaac caaacaattt catgcttcct tctatggagt
     1801 agatggtaaa aagatgaaac tgaccagcaa tgacgaagtc aaggaagtct tgtctcgtct
     1861 gacgagtaaa gacttttcag tagatcaggt ggataagaaa gagcgcaagc gcaatgctcc
     1921 tttaccctat accacttcat ctatgcagat ggatgctgcc aataaaatca atttccgtac
     1981 tcgaaaaacc atgatggttg cccaacagct ctatgaagga attaatatcg gttctggtgt
     2041 tcaaggtttg attacctata tgcgtaccga ttcgactcgt atcagtcctg tagcgcaaaa
     2101 tgaggcggca agcttcatta cggatcgttt tggtagtaag tattctaagc acggtagcaa
     2161 ggtcaaaagc gcatcaggtg ctcaggatgc ccatgaggct attcgtccgt caagtgtctt
     2221 taatacacca gaaagcatcg ctaagtatct ggacaaggat cagctcaagc tatataccct
     2281 tatctggaat cgttttgtgg ctagccagat gacagcggcc gtttttgata ccatggctgt
     2341 taaattgtct caaaaagggg ttcaatttgc tgccaatggt agtcaggtta agtttgatgg
     2401 ttatcttgcc atttataatg attctgacaa gaataagatg ttaccggaca tggttgttgg
     2461 agatgtggtc aaacaggtca atagcaaacc agagcaacat ttcacccaac cgcctgcccg
     2521 ttattctgaa gcaacactga ttaaaacctt agaggaaaat ggggttggac gtccatcaac
     2581 ctacgcgcca accattgaaa ccattcagaa acgttattat gttcgcctgg cagccaaacg
     2641 ttttgaaccg acagagttgg gagaaattgt caataagctc atcgttgaat atttcccaga
     2701 tatcgtaaac gtgaccttca cagctgaaat ggaaggtaaa ctggatgatg tcgaagttgg
     2761 aaaagagcag tggcgacggg tcattgatgc cttttacaaa ccattctcta aagaagttgc
     2821 caaggctgaa gaagaaatgg aaaaaatcca gattaaggat gaaccagctg gatttgactg
     2881 tgaagtgtgt ggcagtccaa tggtcattaa acttggtcgt tttggtaaat tctacgcttg
     2941 tagcaatttc ccagattgcc gtcataccca agcaatcgtg aaagagattg gtgttgagtg
     3001 tccaagctgt catcagggac aaattattga gcgaaaaacc aagcgtaatc gcctattcta
     3061 tggttgcaat cgctatccag aatgtgaatt tacctcttgg gacaagcctg ttggtcgtga
     3121 ctgtccaaaa tgtggcaact tcctcatgga gaaaaaagtc cgtggtggtg gcaagcaggt
     3181 tgtttgtagc aagggtgact acgaagaaga aaagattaaa taagaggagg ggcccgaaag
     3241 taaatttcag gctccttttg gttagaactt gacaaaatac atcattttat gcgaaactag
     3301 aagaagatta ttttaactag gagaagttat gcgtcttatc tatctgataa ttggtttttt
     3361 atcactggcc ttggctattg ttggggttgt tttacccttg ttgccgacaa cgcctttcct
     3421 tttgttgtct attgcttgtt tctccagaag ttccaagcgt ttcgaagact ggctttatca
     3481 taccaagctc tatcaagcat atgtagctga ttttcgcgag actaagtcta ttacgcgtga
     3541 acgaaagaaa aaaatcatcg tctctatcta cgtcttgatg ggaatttcta tttattttgc
     3601 acctctttta ccagtcaaaa tcggtctggg tgctttgacc atctttatca cttattatct
     3661 cttcaaggtc attccagaca aagaatagtt aaaacagtag ttatttgcct tgataaaatt
     3721 gaaagcatat tcataacaat atgatataat aaatttgaag taatattcaa ggagaatcaa
     3781 atgatttacg aattttgtgc tgaaaatgtg actttacttg aaaaagcgat gcaggctgga
     3841 gctcgtcgga ttgaactctg tgataatcta gcagttggtg ggacaacacc cagctatgga
     3901 gtgactaagg cagcggttga actggcagct aactacgata caaccatcat gaccatgatt
     3961 cggccacgtg gtggtgactt tgtctataat gacctagaaa ttgctatcat gctagaagac
     4021 attcgtttga ctgctcaggc tggaagtcaa ggggttgtat ttggagcttt aactgctgat
     4081 aaaaagttgg ataagcctaa tctggaaaag ttaattgctg catcaaaagg aatggaaatt
     4141 gtctttcaca tggcctttga tgaactaagt gatgaagatc aaccggaagc tattgactgg
     4201 ctcagtcaag ccggtgtcac tcgtatccta actcgtgctg gtgtgtctgg cgactcctta
     4261 gaaaaacgtt ttgttcacta tcacagaatt ttggagtacg ctaaaggtaa aattgaaatt
     4321 ctaccaggtg gggggattga ccttgaaaac cgtcaaacct ttatcgacca ggtgggggta
     4381 acacaattgc atggtactaa ggttgttttt taaaaaatag aaaggaactg ctagctttgg
     4441 gtagcagttt tcacttatgt ttgaaatttt taaatcctat caatttaatc aagaaaaggc
     4501 tcatgattat ggttttatag aaaatagcga agtctggaca tatagttgcc agattttgca
     4561 aggtgacttt gtcatgactg tgtccatcac tgctgataat gtgaactttc aagtctttga
     4621 ccaagagact ggtgacctct atcctcacgt ttatatggaa agcatgaggg gaagttttgt
     4681 cggaaatgtc cgtgaggctt gtctggagat tctttaccag attcggaagg cttgttttga
     4741 tgtgcaagat tttatctgtc atcagactaa gcgtatcatg actcaagttc aggaaaagta
     4801 tggaaaccag ttggagtatc tgtgggaaaa atcgcctgat acagctgtat tgcgccatga
     4861 aggcaatcaa aagtggtatg ccgtcttgat gaaaatctct tggaataagc tggaaaaggg
     4921 cagagaagga caagtggaag cagtcaacct caagcatgac caagtagcta atttgctttc
     4981 acaaaagggg atttatccag ccttccatat gagcaagcgc tactggatta gtgtgtccct
     5041 tgatgatact ttatcagatg aagaagtact ggaattgata gaaaaaagtt ggaacttaac
     5101 ctctaaaaaa tgaaatattt taataatttt catgaacttt caattagcta aatattcttt
     5161 actgaagaaa tttttagaaa atataggatt taccagacta gaggaatatg gtgccatctt
     5221 caaatacctg attgagaatg tcaagacgga tcgtcagatc atctgttcgc ctcactgtca
     5281 tgatgacctc ggaatggcag tggcaaatag ccttgctgct gtcaagaatg gtgcaggacg
     5341 tgttgaaggg actatcaatg gtattaggga gcgagctgaa aatgctgctt tggaagaaat
     5401 tgcagtggct ctcaatattc gccaagatta ctaccaagta gaaaccagta ttgtcctaaa
     5461 tgagaccatc aatacgtcag aaatggtttc tcgcttctct ggtattccag ttcctaaaaa
     5521 caaagccgtc gttggtggca ataccttctc ccacgaatct ggtattcacc aagatggagt
     5581 ccttaaaaat cctctcactt atgagatcat cacacctgaa ttggttggtg ttaagattcc
     5641 gcttggagaa ttatctggtc gccatgcttt tgttgagaaa ctgagagaat tggccctaga
     5701 ttttacagaa gaggatatca aaccactctt tgctaagttc aaggcactgg ccgataagaa
     5761 gtaagaaatc acagatgcag atactcgagc tttggtagct ggaaccatgg ttgaaaatcc
     5821 agaaggcttc cactttgatg atttacaact tcaaactcat gcagataatg acattgaagc
     5881 gctcgttagc ctagccaata tggatggtga gaaagtcgaa tttaatgcga cagggcaagg
     5941 ttccgttgaa gcaatcttta acgctatcga taagttcttt aaccaatctg ttcgtttggt
     6001 gtcctacact atcaatgcgg taacagatgg aatcgatgcc caggatcggg ttttggtcac
     6061 tgttgaaaac agagatacag aaaccatctt taatgcagca gggcttgatt ttgatgtgtt
     6121 gaaggcttct gctattgcct atataaacgc taataccttt gttcaaaaag agaatgcagg
     6181 tgagatggga cgcagtgttt cttaccacga tatgcctagt gtgtaaagga gaaggctatg
     6241 gcaaagaaaa tagtagctct agtaggagac ggaattggcc cagaaatcat ggaggctggt
     6301 ttagaagttc tggaggctct agctgaaaaa acaggttttg actatgagat tgacagacga
     6361 ccgttcggag gtgcagatat tgatgcagca ggacctccct tacctgatga aacccttaag
     6421 gcaagtaggg aagcagatgc tatcctacta gcagctatcg gtagtcctca gtatgatgga
     6481 gcagcggttc gccctgaaca aggcctgatg gctctccgta aggaactcaa tctttacgct
     6541 aatattcgtc ctgtaaaaat ctttgacagt ctcaagtatt tgtcaccact caaaccggaa
     6601 cgaatttctg gtgtagactt tgtcgtggtg cgtgaattga caggcgagat ttactttgga
     6661 gatcatattc ttgaagagcg caaagcgcgt gatatcaacg actatagcta tgaggaagtg
     6721 gagcggatta ttcgcaaagc ctttgaaatt gcaagaaatc gcagaaaaat cgttactagt
     6781 atcgataagc aaaatgttct agcgacctca aaactctggc ggaaagtagc tgaggaagtc
     6841 gcacaggatt tcccagatgt aaccttggaa caccagctgg tagactcagc tgctatgctt
     6901 atgattacca atcctgctaa gtttgatgtt attgtaacgg agaatctttt tggagatatt
     6961 ttatctgatg aatcaagcgt cttatctggt acacttgagg ttatgccatc agccagtcat
     7021 tctgaaaatg gaccaagtct ctatgaacct attcacggtt cagcacctga tattgcaggt
     7081 caaggaattg ccaatcctat ttccatgatt ttatcagttt ccatgatgtt gagagatagt
     7141 ttcggacgtt atgaggatgc agagcgtatc aaacgtgctg ttgagacaag tctggcggca
     7201 ggaattttaa cgagagatat aggaggtcag gcttcaacaa aggaaatgac ggaagctatt
     7261 attgcaaggt tatgaagtta gacgaaaaaa ttactctagt ccttttgatt tggaatgtca
     7321 tcattttctt gatttatggt attgacaaat ctaaggcaag gagaagagtt tggcgcatcc
     7381 ctgagaaaat cttacttatt ttagccttta cttttggtgg ttttggtgcc tggctagcag
     7441 gaatcatctt tcaccacaag actcgaaaat ggtactttaa aatagtttgg tttcttggga
     7501 tggtgaccac actagtagcc ttatatttta tttggaggta atggatggca gggtcttcga
     7561 gggaatacgc tgcttgggct ctagcggact atggttttaa ggtcgtgatt gcaggatctt
     7621 tcggtgacat tcattacaat aatgaactca ataatggcct gttgccaatc gttcagccta
     7681 gagaggttag agagaaacta gcccagctaa aaccaaccga ccaggtaact gtggacttgg
     7741 aacaacaaaa aatcatctca ccagttgaag aattcacctt cgagatagat agcgggtgga
     7801 aacataaact cctaaatagt ttggatgata tcggtattac cttgcagtat gaagagttga
     7861 ttgctgctta tgaaaaacaa cgaccagcct actggcagga ttagaaaaat agaaaaggag
     7921 atatagtaaa ctgaaataag atgtaaacaa atgaattgga gcttaacatc catttccagc
     7981 aattttttag aaactacagt ggactattct ggattcaaca cattataaaa ttatgacaaa
     8041 acacattcac aagaaggcta cgacatttta aaaggtgagg gcggatgtat cgtttgccct
     8101 actaaagttg gttacattat catgaccagt gacaaggcag gacttgagcg taagttcgca
     8161 gccaaagaac gtaagcgtaa caaaccaggt gttgttctct gcggtagcat ggatgaactt
     8221 tgcgctttag cgcaactcaa cccagaaatt gaagcattct actaaaaaca ttgggatgaa
     8281 gatattcttc ttggttgtat ccttccttgg aaaccagaag cctttgaaaa actcaaagca
     8341 tacggggatg gccgtgaaga acttattact gatgtacgtg gtactagctg ttttgttatc
     8401 aagtttggaa aagcaggtga acaattggct gccaagcttt gggaagaagg taaaatggtc
     8461 tacgcctcat ctgcttcaat gacaaaacga ttgaaactcg ctatgagcaa ggtgtaatgg
     8521 tgtctatggt cgataaggac ggcaaactca tcccagaaca aggaggagca cgttcaactt
     8581 caccagctcc agttgtgatc cgtaaagggc ttgacattga taaaatcatg atgcacctgt
     8641 cagatacttt taactcatgg gactaccgtc aggttgagta ttattaggat agagaagaag
     8701 tctagtgtta tgagatatta aagctcctaa cactgggctt ttgtttagaa tttcttttct
     8761 ttttctatag gatatggtat tctatgtaga aaatatatgt taataagtaa tgccaatatt
     8821 taaacatcat tagtaaaagg agttagattg atgaataaaa gaaaagttag tttagaag
//
LOCUS       DAVGJL010000087         8858 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.088, whole genome shotgun sequence.
ACCESSION   DAVGJL010000087 DAVGJL010000000
VERSION     DAVGJL010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8858
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.088"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(144..707)
                     /locus_tag="VVE42_001545"
     CDS             complement(144..707)
                     /locus_tag="VVE42_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU3073722.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGVTLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILTLAIVPRLQTLKK"
     gene            complement(691..1242)
                     /gene="coaC"
                     /locus_tag="VVE42_001546"
     CDS             complement(691..1242)
                     /gene="coaC"
                     /locus_tag="VVE42_001546"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEU3073723.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLEKQGHQVTVLMTQAATEFIQ
                     PLTLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLIAPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETIDEKTL"
     gene            complement(1254..1943)
                     /gene="coaB"
                     /locus_tag="VVE42_001547"
     CDS             complement(1254..1943)
                     /gene="coaB"
                     /locus_tag="VVE42_001547"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEU3073724.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLFAGHEVC
                     LITTKRALKPEPHPNLSIREITNTKDLLIEMQERVQDYQVLIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQAKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISAGQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            2173..3843
                     /locus_tag="VVE42_001548"
     CDS             2173..3843
                     /locus_tag="VVE42_001548"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEU3073725.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELGIDQRR
                     ILWKRVVDLNDRALRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATLRAL
                     KMNGGVAKDALTEENVEAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAVL
                     KELCASIDVPVELASVWADGAEGGVALAETVVKTIAENPANYKRLYDNDLSVQEKIEK
                     IVTEIYRGSKVNFEKKAQTQIAQIVQNGWDKLPICMAKTQYSFSDNPNALGAPENFEI
                     TIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF"
     gene            4171..5346
                     /gene="mutY"
                     /locus_tag="VVE42_001549"
     CDS             4171..5346
                     /gene="mutY"
                     /locus_tag="VVE42_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEU3073726.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATAPEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     FIEVDNFSQEEQFDLFHQVAEESVNSGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQAYAQANLDSSQD"
     gene            5401..6105
                     /locus_tag="VVE42_001550"
     CDS             5401..6105
                     /locus_tag="VVE42_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU3073727.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            6098..7447
                     /gene="vicK"
                     /locus_tag="VVE42_001551"
     CDS             6098..7447
                     /gene="vicK"
                     /locus_tag="VVE42_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEU3073728.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQI
                     NQKVKDLIAGDYSKVLDMQGGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     HLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            7449..8258
                     /locus_tag="VVE42_001552"
     CDS             7449..8258
                     /locus_tag="VVE42_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU3073729.1"
                     /translation="MSEIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
     gene            8349..8591
                     /locus_tag="VVE42_001553"
     CDS             8349..8591
                     /locus_tag="VVE42_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEU3073730.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE"
     gene            8593..8847
                     /locus_tag="VVE42_001554"
     CDS             8593..8847
                     /locus_tag="VVE42_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEU3073731.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQTNVLEDPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHRRDIY"
BASE COUNT         2656 a         1570 c         2006 g         2626 t
ORIGIN      
        1 ctgtcctgat cgatttgtcc tgttcgattt gtcctgttcg atttgtcctg ttcttatttc
       61 gttttactat agtaaagatt tcattaaaaa gaaactgtat agagcaaaat ctccaccttc
      121 aggtttgaaa gcggagattg tttttatttt ttcaaggttt gtagtcgtgg aacaatggct
      181 agggttagaa ttgcagaaat gaccaattca gcaattgaat ttgttgagat aacggttgcc
      241 agaagaagtt ggatatttcc attataaaca tttccaaata ggaagaagat tcctccaagg
      301 acaaagatag tatttgtcaa ggaaccaaga gccccagcta aaatcagacc agtcttgttt
      361 ttcatcagtt tatagactaa gtaaggagtt aaaccaatca aaatacgtgg gacaatggca
      421 atgatagctg agtagatgtt tccgtttggt acgaagggag agaagaggta gcttgtcggt
      481 agaatcgtaa tcgtgttaac cgtcaagcta agtaatccca tcaaaaatcc aagtgtaacc
      541 ccaactcgtg gaccataaat aatgctggca ataatgacag gaatatgaac aatggtcggt
      601 ttgattggaa atggaaaaag gttaaagata agtgagctca gaaagtgtat cacgagcatg
      661 gtagcaaaaa agatagcaat gggtgcaata ttagagcgtt ttttcatcga tagtttcctt
      721 tattctttct aaaataattg tgaggtcagc taaagctcct cgtccgtggt ctccacaagc
      781 tagtagggat tccttaggag caatcagctg atagccgtag gtttctaatg ttttcagatt
      841 attctgagtt actggatggt catacatttt tgtattcata gcaggagcta ttagtttggg
      901 aatatgactt ggtagggcta gagctgtact ggttaccatg ttgtccgcaa atccgtgagc
      961 tagttttgca atagtgttag cagttgcagg taccacgata aataaatctg ctttttttcc
     1021 aagttcgata tgattgacct gatcaggata gggttccttc atgacatcca agtggacagg
     1081 attctgtgag agtacctgta gtgtcaaagg ttggataaac tctgtagcag cctgagtcat
     1141 taagacagtg acttgatggc cttgtttttc tagagaactg actaaatctg ccgacttata
     1201 agaggcgatt gaacccgtta cagccaagag aatgtttgcc atagttttcc tttctaagaa
     1261 tgataggctt gaattttttc aaggaggagt tctgcaattt cttctttagt ctggactgtt
     1321 tgaagctgat ttttctcaac aaatatagct cggtgctgac ctgctgaaat ttgagtcagg
     1381 tcattcgcga tgattaaatc tgcttgattc ttgataagac tttttcgtgc aatgtcaacc
     1441 agatgatctt cggtaacatc aaccagcagt ttgaaaccaa tcagatgaat agtaggattc
     1501 cattccttga ctagggatat gattttgggt gtctttttaa ggaacaaaac ctgaacctca
     1561 tcagttgaag aaatcttggc ctgatgattc tgcttgctta aaaattcttt tagattggag
     1621 ctagcctgaa cttcctcaag ccctgtcata taaacaggag tgtagtcaga aacagccatt
     1681 gagtggatca agacctgata atcctgaaca cgttcttgca tttctattag aaggtccttg
     1741 gtattggtaa tttctcgaat acttaggtta ggatgaggct ctggcttcag agctcgtttt
     1801 gtcgtaatta aacaaacttc atgccctgca aaaagcaaag tctctgtgat aattttcccc
     1861 aagtgacctg tagaatggtt agtgatagag cggacgctat cgatagcttc actggtaccg
     1921 cccgatgtaa ctaaaatttt catagcacta ttgtacacaa aaataagcgg taagtaaaat
     1981 gaaagtgata aatttttggt aaaaacgaag atttactata gtttcaaact ataaagccat
     2041 ataaaattta agaaaggact ctaaaagcct taaaaacaga gatatttacg aacgtttaga
     2101 gagttttagg atgttaaaaa tcttgttttg tgatataatg aattatcata taagaggtta
     2161 gaggaatttt gaatgaaaac agatattgaa atcgcacaga gtattgagtt gaagccaatt
     2221 gttgatgttg tagagaaact tggtatttct tacgacgatt tggagttgta cggaaagtac
     2281 aaggctaaac tcagctttga taaaattcgt gcagttgaga gtaatccagt cggtaaattg
     2341 atcttggtta ctgccatcaa cccaacacct gcaggtgaag gaaagtcgac gcttaccatt
     2401 ggtcttgcgg atgccttgaa caagattggc aagaaaacca tgattgctat ccgcgaaccg
     2461 tctcttggtc cagtcatggg gatcaagggt ggtgctgctg gtggtgggta tgcacaagtt
     2521 ctgccaatgg aagacatcaa cctccacttt actggagata tgcatgctat tacaactgcc
     2581 aacaatgccc tttctgcctt gattgacaac cacttgcacc aagggaatga gctgggaatt
     2641 gatcaacgtc gtatcctctg gaaacgcgtt gtggacttga acgaccgtgc gctccgccat
     2701 gtgactgttg gtcttggtgg tcctctaaac ggtattccac gtgaggatgg ttttgatatt
     2761 acagttgctt cagaaatcat ggcaattctt tgcttggcaa cggacatcga ggacttgaaa
     2821 cgtcgtttgg cgaatatcgt tattggttat cgctatgacc gtacgcctgt ttctgtaggt
     2881 gatttgcagg ttgagggtgc cttggctttg attttgaagg atgctattaa gccaaacttg
     2941 gttcagacaa tttacggtac acctgccttt gtacacggtg gtccatttgc caatatcgct
     3001 catggctgta actctgtttt ggcgacgaca acagcccttc acttggctga ttacactgtt
     3061 actgaagctg gttttggtgc ggatcttggt gctgagaaat tccttgatat caagacacca
     3121 aacttgccaa catctccaga tgcagttgtt attgtcgcaa cccttcgtgc ccttaagatg
     3181 aatggtggtg tggctaaaga cgctctgact gaagaaaatg tagaggcagt tcgtgcaggt
     3241 tttgctaact tgaaacgcca cgttgaaaat atccgtaagt tcggtattcc agcagttgta
     3301 gctattaacg aatttgtatc tgatacagaa gctgaaattg cagtcttaaa agaactctgt
     3361 gcctcaatcg atgtaccagt tgaattggct agtgtctggg ctgatggagc agaaggtgga
     3421 gtagcacttg ccgaaacagt tgttaagaca attgctgaaa atccagctaa ctataaacgt
     3481 ttgtacgata atgacctttc tgtccaagaa aagattgaaa aaattgtcac tgaaatctat
     3541 cgtggtagca aagtgaactt tgagaagaaa gctcaaacac aaattgctca aatcgttcaa
     3601 aacggttggg acaaattgcc aatttgtatg gctaaaactc aatacagttt ctcagacaat
     3661 ccaaatgcgc ttggagcacc agaaaacttt gaaattacca ttcgtgaatt ggtaccaaaa
     3721 ttaggtgcag gcttcatcgt tgccttaact ggtgatgtta tgaccatgcc aggtcttcca
     3781 aaacgtccag cagctctcaa catggatgtt gaaagcgatg gaactgtact aggcttgttc
     3841 tagtggattg aaattgactt taagtgagtt tggaaataat atccaagctc attttttctc
     3901 agactgcaag cagttcatca ataagattta tactcttcga aaatcttttc aaactacgtc
     3961 agcttcacct tgccgtaggt atatgatact gactctgtca gttctatcca caacctcaaa
     4021 acagtgtttt gagcaacctg cggctagctt cctagtttgc tctttgattt tcattgagta
     4081 taagtagata gaaaagttat atactatcta ttgaaaatat tataaatcag atacaaagac
     4141 aggtgaaacc ttctgtgtta tactagagat atgttagatt tgaaagaata cggtatcgtc
     4201 atgtggccgg aggagaaagt catttctttc cgtgagaaac ttctcgcttg gtatgatgaa
     4261 aacaaaagag atttgccttg gaggagaagt aaaaatcctt atcacatctg ggtatctgaa
     4321 atcatgcttc agcagaccag ggtggataca gttatccctt actacgaaag atttttggac
     4381 tggtttccaa ctgtcgaaag tctggcaact gcgcctgagg agagtttact gaaagcttgg
     4441 gagggcttgg gctattattc tcgagttcgc aatatgcagg ctgcagccca gcagattatg
     4501 actgactttg gtggccaatt tccaaatacc tatgaaggaa tttccagctt gaaagggatt
     4561 ggaccttaca cagcaggagc catttccagt attgctttta acttgcctga gccagctgta
     4621 gatggtaatg tcatgcgggt cttggcgcgt ctgtttgaag tcaaccacga tattgggatt
     4681 ccaagtaatc gaaaaatttt tcaggcaatg atggagatct tgattaatcc ggatcgtccg
     4741 ggtgatttta atcaagcatt gatggactta gggtctgata ttgagtctcc tgtaaatccc
     4801 agacctgaag aaagcccagt caaggacttt agtgcggcat atcagaatgg aaccatggac
     4861 cgttatccaa ttaaatctcc taagaaaaag ccggttccta tttatcttaa agccttagtt
     4921 gtgaaaaatt ctcaaggaca atttttactt gaaaaaaatg aaagcgaaaa gctcttggca
     4981 ggtttttggc atttcccctt tatagaagtt gataactttt cgcaagaaga gcagtttgac
     5041 ctctttcatc aggttgcgga agaaagtgtg aactctggcc ccagtccaga agagagtttc
     5101 cagcaggact atgacctaga tgttgattgg cttgatgttt gttttgatac agtccagcat
     5161 gtttttagtc atcgaaaatg gcatgtacag attgtagcag gtcaggtgag tgacttccat
     5221 gatttttcag atagggaagt tcgttggctt tcaccagaag aattcaagaa ttacccactt
     5281 gccaaacccc aacaaaaaat ctggcaggct tatgcacaag ccaacttaga cagtagccaa
     5341 gactagctat tgtgtcttct gactattttt tggtataata gtagagaaaa aggtgaacat
     5401 atgaaaaaaa tactaattgt agatgatgag aaaccaatct cggatattat caagtttaat
     5461 atgaccaagg aaggttacga agttgtaact gcttttaatg gtcgtgaagc gctagagcaa
     5521 tttgaagcag agcaaccaga tattattatt ctggatttga tgcttccaga aattgatggt
     5581 ttagaagttg ctaagaccat tcgtaagaca agcagtgtgc ccattcttat gctttcagcc
     5641 aaagatagtg aatttgataa ggttatcggt ttggaacttg gggcagatga ctatgtaacg
     5701 aaacccttct ccaatcgtga gttgcaggcg cgtgttaaag ctcttctgcg tcgttctcaa
     5761 cctatgccag tagatgggca ggaagcagat agtaaacctc aacctatcca aattggggat
     5821 ttagaaattg ttccagacgc ctacgtggct aaaaaatatg gcgaagaact agacttaacc
     5881 catcgtgaat ttgagctttt gtatcattta gcatcgcata caggtcaagt catcacgcgc
     5941 gaacacttgc ttgagactgt ctggggttat gactattttg gtgatgtccg tacagttgat
     6001 gtgactgtac gacgtctgcg tgagaagatt gaagatacgc ccagccgacc agagtatatc
     6061 ttgacgcgcc gtggtgtagg gtattacatg agaaataatg cttgatttac tgaaacaaac
     6121 catttttacc agagatttta tctttatcct gattttgtta ggtttcatcc ttgttgtgac
     6181 cctcttatta ctggaaaata gacgtgataa tattcagttg aagcaaatca atcaaaaggt
     6241 taaagatttg attgcaggag attattccaa ggttcttgat atgcaaggtg ggtctgaaat
     6301 caccaatatt accaataatt tgaatgactt gtcggaggtt attcgtctca ctcaggaaaa
     6361 tctagaacaa gagagtaaga ggctaaatag tattctgttt tatatgacag atggggttct
     6421 tgcgaccaac cgtcggggtc agattatcat gattaacgat acagccaaga agcaactggg
     6481 gttggttaag gaagatgttc tgaatagaag cattttggaa ttgctcaaga tagaagaaaa
     6541 ctatgaattg cgtgatttga ttacccaaag tccagaattg ttgctagatt cccaagatat
     6601 caatggcgaa tatttgaacc ttcgagttcg ctttgccttg atacgtcgag agtctggctt
     6661 tatttcaggt ttggtggctg ttttgcatga tacgacggag caggagaagg aagaacgcga
     6721 acgaagactc tttgtttcca atgttagcca tgagttacgg actcctctga ctagcgtaaa
     6781 atcctatctt gaagccttgg atgagggggc tttgtgtgaa actgtagcac cagactttat
     6841 caaggtttct cttgatgaga ccaaccgtat gatgcgcatg gtgacggatc tcctccatct
     6901 ttcacgtatt gataatgcta ccagtcacct agatgtggaa ctgattaact tcactgcttt
     6961 tattaccttt atcctcaatc gttttgacaa gatgaaagga caggaaaagg agaaaaaata
     7021 tgagttggtg agagattatc ccatcaattc tatctggatg gaaattgata cagataagat
     7081 gacgcaggtt gtcgacaata ttttaaataa tgctattaag tattcgccag atgggggtaa
     7141 aatcactgtc agaatgaaga caactgaaga ccagatgatt ttatccattt ctgaccacgg
     7201 tttggggatt cctaagcagg atttaccacg tatctttgac cgtttctatc gtgtggatcg
     7261 tgctagaagt cgtgcacaag gtggtacagg tctaggactg tctatcgcta aagaaattat
     7321 caaacaacat aagggcttta tttgggccaa gagtgaatac ggcaagggtt caacctttac
     7381 cattgtgctc ccttatgata aggatgcagt gaaagaagaa gtatgggagg atgaagtaga
     7441 agactagaat gagtgaaata ggctttaaat acagtatttt agcgtcgggt tccagtggaa
     7501 attcttttta tctggaaacc tcaaaaaaga agcttttagt agatgcaggc ttgtctggca
     7561 agaaaattac cagtctgcta gctgaaatta accgtaagcc agaagacctg gatgccatct
     7621 tgattaccca tgagcattca gatcatatcc atggagtagg cgttttggct cgcaagtatg
     7681 gtatggatct ttatgccaat gaaaagacct ggcaagctat ggaaaatagt aaatatcttg
     7741 gcaaggtgga ttcttcgcaa aagcatattt ttgaaatggg taaaaccaaa acctttggag
     7801 atatcgacat cgagagtttt ggtgtaagcc atgatgcagt cgcaccgcag ttctatcgct
     7861 ttatgaagga tgataagagt tttgtcctct tgacagatac aggttatgtc agtgaccgta
     7921 tggcgggcat tgtcgaaaat gcggatggtt atcttatcga ggccaaccat gatgtagaga
     7981 ttttgcgatc aggttcttac gcttggcgac tcaaacaacg aatcctatct gaccttggtc
     8041 acctttctaa cgaggacggt gctgaagcta tgattcggac gctaggaaat cgtactaaga
     8101 agatttacct tgggcattta tctaaggaaa acaatatcaa ggaactggct catatgacca
     8161 tggtcaatca gctggctcaa gctgatctgg gagtcggagt agactttaag gtttatgata
     8221 cctcaccaga taccgcaaca ccattgacag agatatagaa agaacgctga gaaggtgttc
     8281 tttttatatt gactggacac ctaaaaagta atacaatggt gttaccatta aaaaagggag
     8341 cacaaaagat gactactatt acattaaaag tttctgaagc tgataaaaca tttatgaaag
     8401 caatggctaa gtttgaagga gtttcccttt cggaacttat tcgcaccaaa actcttgaag
     8461 ccctagaaga tgaatacgat gctcgtgtgg cagatttagc ctatcaagag tatttagaag
     8521 acttggaaaa aggagttgaa cccattactt gggaagaaat gatgcatgat ttaggcttga
     8581 aggatgaata atttgtataa attagttcca acaagacgtt ttatcaagca attgaaaaaa
     8641 ttggaccgtt atacgcagaa gctaattaca aactatttac aaaccaatgt tttggaagat
     8701 ccaagacgac acggaaaggc tttggttggt aatcgcgttg gtcaatggcg ctatagaatt
     8761 ggtaattatc gagttatcgt acaaattgta gatgatgaat tagttgttgc tactctagaa
     8821 gttggtcatc ggagagatat ttattgaatt aaagctga
//
LOCUS       DAVGJL010000088         8704 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.089, whole genome shotgun sequence.
ACCESSION   DAVGJL010000088 DAVGJL010000000
VERSION     DAVGJL010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8704
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.089"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(31..921)
                     /locus_tag="VVE42_001555"
     CDS             complement(31..921)
                     /locus_tag="VVE42_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEU3073732.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGIFGSSAVLADSVHDLVDA
                     IAIGISAFLETISNREEDNQYTLGYKRFSLLGALVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINMLASLVVGKGKTKNESILSLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLVISFFILSKALPRFWSTLKIFLDAVPEGLDIKQVKSGLERLDNV
                     ASLNQLNLWTMDALEKNAIVHVCLKEMEHMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
     gene            1054..1590
                     /locus_tag="VVE42_001556"
     CDS             1054..1590
                     /locus_tag="VVE42_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU3073733.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKGYEATTVQDIIDLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFHKNQDHITSLLFS
                     KNDYFLRQLQRELEHHVYSVLADNLKEAHPNLPTSYLQHLVMSNFIETLTWWLKKGQD
                     FTDQEVVQFYLDLLIPKN"
     gene            complement(1756..2562)
                     /locus_tag="VVE42_001557"
     CDS             complement(1756..2562)
                     /locus_tag="VVE42_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEU3073734.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSGTMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELIYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDGKGWTKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLLN"
     gene            complement(2907..4427)
                     /locus_tag="VVE42_001558"
     CDS             complement(2907..4427)
                     /locus_tag="VVE42_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEU3073735.1"
                     /translation="MTNLIATFQDRFSDWLTALSQHLQLSLLTLLLAILLAIPLAVFL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLKGIDPNLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLAALIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVAL
                     LLGLSYSPALLVQKEKENLVIAGKIGPEPEILANMYKLLIEENTSMTATVKPNFGKTS
                     FLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDGIAKQDHLAYLKP
                     MSYQNTYAVAVPKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVATMEPALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELERVLNTLAGKITESQMSQLNYQVGVEGKSAEQVAKEFLQEQGLLKK"
     gene            complement(4420..5148)
                     /locus_tag="VVE42_001559"
     CDS             complement(4420..5148)
                     /locus_tag="VVE42_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3073736.1"
                     /translation="MIEYKNVALRYTEKDVLRDVNLQIEDGEFMVLVGPSGSGKTTML
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLSTGYVLQAIALFPNLTVAENIALIPEM
                     KGWSKEEITKKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKIFLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVA
                     NPETILKVPATDFVADLFGGSVHD"
     gene            complement(5163..5927)
                     /locus_tag="VVE42_001560"
     CDS             complement(5163..5927)
                     /locus_tag="VVE42_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU3073737.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGDTYIQHSTGVPDGK
                     EGFAAFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFRADENG
                     RIVEHWDVIDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTEIFQNGELEQW
                     SDYVADDLIQHNHEIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAQYDIFRLENGKIVEHWDNKEVMPKVEDLTNRGKF"
     gene            complement(5931..6374)
                     /locus_tag="VVE42_001561"
     CDS             complement(5931..6374)
                     /locus_tag="VVE42_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU3073738.1"
                     /translation="MKDSHLLAHHIRLLNGRIFQKLLSQDPEALYRGEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKKLEEQKLVIVSPCGKDKRKKYLVLTELGKSQ
                     KEVGHRVSQKLDTIFYKGFSEEEIHQFEGFQERILANLKEKGNEV"
     gene            complement(7056..7352)
                     /locus_tag="VVE42_001562"
     CDS             complement(7056..7352)
                     /locus_tag="VVE42_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEU3073739.1"
                     /translation="MVEPNLESLIKDLYNHARHDLSEDLVAALLETTKKLPTTNEQLQ
                     AVRLSGLVNRELLLNPKHPAPELLNLARFIKREEAKYRGTATSALMYEELFKML"
     gene            complement(7374..8438)
                     /gene="pepA"
                     /locus_tag="VVE42_001563"
     CDS             complement(7374..8438)
                     /gene="pepA"
                     /locus_tag="VVE42_001563"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEU3073740.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSGQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKAETESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
BASE COUNT         2582 a         2064 c         1496 g         2562 t
ORIGIN      
        1 agatagtacc caagtatttt tcactttttt ctaatgttga tgctcataac tccgttccaa
       61 gtcacacacc tttcgcttat gggtttggtg agtttctagg tcagcatcaa tttcaatggt
      121 aatattttga aaaccacaat cttttaggaa aattcgaata gactctttac aagtttccat
      181 atgttccatt tcttttagac aaacatggac aatggcattt ttttccaaag catccatagt
      241 ccagagatta agctgattaa ggctggccac attgtccaat cgctccaggc cactctttac
      301 ttgcttgata tcaagacctt ctggcacagc atccaagaaa atcttgagtg tagaccaaaa
      361 acgtggaagg gcttttgaaa gaataaagaa agaaatgaca agggacaaaa gaggatctag
      421 gatataccag tccgtaaatc gaagaacaat cgccatcagg ataacagcta cccaccctag
      481 cgtatcttcc agaaaatgca gactcagaat agactcattc tttgtctttc ccttaccaac
      541 caccagactc gctaacatat tgatagtaat cgcaataatt cctaaccaga gaatcccctc
      601 atcattgact ggttgcggat gcaaaatctt cgtgacattt tccaaaatga ctagaacaga
      661 gcccgttacg agaatcacag ctgttaccaa ggctcctagc aggctaaacc gcttatagcc
      721 caaggtgtac tgattgtctt cttcacgatt ggagattgtt tctaaaaaag ctgatattcc
      781 aattgcaatc gcatctacca agtcatgcac agagtcagca agaacagcgc tagaaccaaa
      841 tattccacct gcaataaact caacaatggc ataagtcaaa tttaagaaaa aagccaccca
      901 aacagcatat tttgccttca tatttctcat tcctttgtta taatagattt atgaacacct
      961 tgttcattat cattatccac taaaactcaa agaaaggata gaagcaaaac gtcagcttta
     1021 ttcagttctg aacaatttgc cttaagtgtc catatgacta acattgaccg ccgtatcagc
     1081 aaaaccaaaa aagccatcta tcaagctttt atacaacttt tgaatgctaa gggctacgag
     1141 gccactactg ttcaggatat cattgatctc gcagatgtgg gacgatccac cttttactgt
     1201 cactatgaga gtaaggagct tcttctggac cagctctgcc gctacctctt ccatcatctt
     1261 tttgaaagag agcaagccat ttcaaccgag gactacctcg cccacctctt tctccatttt
     1321 cataaaaacc aagaccatat caccagtctg ctattttcca aaaatgacta cttcctccgt
     1381 caactccaga gagagcttga gcaccacgtc tattccgtgc tagctgataa tttaaaagaa
     1441 gcccacccta atctgcctac ttcttacctc caacacttgg tcatgtccaa ctttatcgag
     1501 acattgacct ggtggctcaa aaaaggtcaa gatttcacag accaggaagt tgtccaattt
     1561 tatctagacc ttctcattcc taaaaattga atatagagta aagctcagtt gtcttatttc
     1621 tagattactg agttttttat cttttcaaca acaaaagagg acccgccgat cctctttttc
     1681 atactataaa tccttgatta tcaactatat ctgttttaat cgaaatctca aaacagcact
     1741 ttcaaacatc ttttcctagt taagtaaatc agtattctgc ttagctgcct tgctccattg
     1801 ataccaacca actagactgt taatgagata aattagatat ttcccttgaa tttgcaggct
     1861 ttctccccac cagagataga ttgaaaagac attggtagcc gcccagaata tccactgttc
     1921 acggtaaaca gctgtcatga ggatttgccc taccccattg gttgcatctg tgattgaatc
     1981 acgataggga cgattggcac caatagactg ataaatgaag ccaaaggcca accaccaaag
     2041 cacactaatg gaaagatact ttgtccagcc cttgccgtcc agtttacgcg cgacaaactc
     2101 ctgcttttcc ttcttaaact gtgcctgata aatccaaact agaagtccaa ttggctgcat
     2161 gactgtgaag taaagtgtcg tcagcacctc accataaaag cctttctgta gggccaaaat
     2221 aaggtaaata acagagttaa tcaagccaaa aagataatta cttgctcgac cttccgatac
     2281 aaagattaca cagataatcc ctgtcaagct acaaatcatc ccaatccagt caacaatacg
     2341 atgttcgtaa atcaactcca gccagagagg aaaacttcct aaaaccagca aataaatcca
     2401 ctgggcaaaa ctacgatggg caaagaggtc atcccagata gccttcatag ttcctgaaaa
     2461 tcctaaatca gccatagccg caaccatacg acggtaacca cctgacattt cacctagggt
     2521 tgttttgata ttttcaattt tcttttgcaa ataagtatgc atcatttctc cttttgtttt
     2581 taaagagccg tgtctggata gactttcgga cgcaacgctc tattagataa tgaactgcct
     2641 atacacaaga tttctaacct tagtcgacat gagctgaaac cccttatttg ttaagtagtt
     2701 cacaaaatat tatacatcta ttttatgaat agtcaagtgt ctttacagta aaattttaga
     2761 aaatcatgaa aattttctct ttctttccat tttaagtgac attcagtcat tctcacatca
     2821 aaaaagccca gacgaaattg tctgagcatt cttttatcta gtcgtttaag gaagttgagt
     2881 tcagtatgtt taaagtctct gtcccatcat ttcttcaaca aaccttgttc ttggagaaac
     2941 tccttggcta cttgctctgc tgacttgcct tcaacaccga cttggtagtt gagctggctc
     3001 atctggcttt ctgtaatctt accagccaat gtattaagaa ctctttccaa ctctgggtgt
     3061 ttcttgagaa gagcttcttt catgagtgga gccccttgat aaggtgggaa gagttgcttg
     3121 tcatcttcca agacctgtaa atcataacgc tccaattccg catcagtcga ataggcgtcc
     3181 gtaatttgaa tatcacctga ctgaatagcc tgatagcgaa gggctggctc catggtcgct
     3241 acattgagat tgagaccata cattgattgc aagcccttat ttccatcttc acggtcgtta
     3301 aactcgagtg taaaacctgc cttcaactgc ccttccactt ttttcaagtc tgaaatggtc
     3361 ttcaagccat attcttgagc aatctttttc ggaacagcta cagcataggt gttttgataa
     3421 gacatgggtt tgagataggc tagatgatcc tgcttagcaa tgccatcacg cgccacctga
     3481 taaacctgtt ctggttcatg actcaccttg ggtgatggtt gaagcaaact ttcagtcacc
     3541 gtaccagtaa attcaggata gatgtcaata tcgccttttt tcagagcttc ataaaggaag
     3601 cttgtcttcc caaaattcgg tttaacagtc gcagtcatgc tggtattttc ttcaatcagc
     3661 aacttataca tattggccaa aatttctggt tctggaccta ttttcccagc aataaccaag
     3721 ttttccttct ctttttgaac caaaagagct ggactataag acagacccag taataaagcc
     3781 accaaggcaa aacctgagaa aatcgtccgt aattttgctt tttccattac ttttagtagg
     3841 aagttaaagg caatagcaag cactgcagaa gaaagtgccc caatcaaaat caaactggca
     3901 ttattacggt caattcccaa caggataaag gaacccagtc cccctgctcc aatcaaggcc
     3961 gccaaggttg ccgtaccgat aatcaaaaca gctgccgtcc gaatcccaga catgataaca
     4021 ggcatggcga gtggaatttc aaatttcttg agacgttccc atctggtcat cccaaaggca
     4081 atcccagcct cttgcaggtt cggatcaatt cccttcagcc cagtgatagt attttgcaaa
     4141 atagggaaaa tcgcataaat cactagagct gtcaaagccg gcaaggtccc aattcccatc
     4201 aaagggataa agagccccaa caaggccaga gacgggatgg tctggaaaat acctgcaatc
     4261 tgcaagaccc agtcggccag cttctcatga tagcgaagaa aaacagccaa gggaatcgca
     4321 agcaaaatag ctagtaacaa ggtcaaaagt gacaactgca aatgttgaga tagagctgtc
     4381 aaccaatcac taaaacgatc ctgaaaagtt gcaattaaat tagtcatgaa cactacctcc
     4441 aaacaagtct gctacaaagt ctgttgcagg cacttttaaa attgtctcgg gattcgctac
     4501 ctggcgaatt tctccatcct gcaagacagc aatacggtcc gccaacttca aggcttcatc
     4561 cgtatcatgg gttacaaaaa tcgttgtcat cccaaactct ttatgcaatt cttttgtcag
     4621 aacctgcaac tgttttctcg aaatagcatc caaggccgaa aagggttcat ccatgaggaa
     4681 aatcttgggc tgaccaatca tagctcggac aataccgacc cgttgctgtt ctccaccaga
     4741 taattcacta ggtaagcgat gcccatactc ggctactggt aaaccaacct tagccaaaag
     4801 ctcttctgtt ttcttcgtaa tttcttcctt gctccacccc ttcatttcag gaatgagagc
     4861 aatattttcc gcaactgtta gatttggaaa aagagcaata gcctgtaaaa cataaccagt
     4921 agaaagacga agttcacgct catcatagtc tttgatgcgc ttcccatcca tataaatatt
     4981 tccatcagtt ggttccaaaa gacggttaat catcttgagc atggtcgtct tacctgaccc
     5041 agaaggccct actaaaacca taaattcccc atcctcaatc tgtaagttga catctctcaa
     5101 gacatccttt tctgtgtagc gcagtgctac atttttgtat tcaatcattc tttgtcctca
     5161 atttaaaact tccctcgatt agtcaagtct tctaccttag gcatgacttc cttattatcc
     5221 caatgctcca caattttccc gttctctaaa cggaagatat cgtactgggc ataagcaacg
     5281 ccatcaatct gagtctgacc atagctaacc acatagtttc cttgtcctaa gagttggaaa
     5341 acaaagtcaa aagtgacact atattcagcc acatagtttt tataagcagc acttccttgt
     5401 ccaatctcat gattatgctg aatcaaatcg tctgccacat aatcactcca ctgctctagc
     5461 tccccatttt ggaaaatttc tgtcaagaaa cggcgaacca gctttttatt ttctgctttc
     5521 ttatccaaat ccttgatttc aaaatctcca aaaatttgat ctagttgatc attttcaggt
     5581 gttcgatagt agtcaatgac atcccaatgc tcaacaatac gaccattctc atccgcacgg
     5641 aaagtatccg tcgtcaccca ttgagcttct ccaccattca gatattgatg aacatgaaca
     5701 aagaccagat tgccatcctc aatggtgcgg acaatcttaa tctgacgctc tggatgacgc
     5761 tcaaagaaat ctgcaaagaa ggctgcaaat ccttctttcc cgtcaggaac acctgtcgaa
     5821 tgttggatat aggtatcccc tacagactgg gcttgagcct cagcaactcg tccgtcttga
     5881 atggcatgga tgtataggtt gtgagcattt ttcacttgtt gtgacatatt ctaaacctca
     5941 tttcccttct ctttcaaatt cgccaaaatt ctttcttgaa aaccttcaaa ttggtgaatt
     6001 tcttcctctg aaaatccttt gtaaaagata gtatccaatt tctgactgac acgatgcccc
     6061 acttctttct gggacttgcc taactccgtt aaaactaaat acttcttacg cttgtctttt
     6121 ccacacggac taacaattac aagcttttgt tcctctagct tttttatcat agtcgtcagc
     6181 gtattattcg caagcccagt cgcaagcgcg atatctgtcg cagttgcgca gccagtttca
     6241 ctattccata aaaccgctaa aatcttgccc tgttcacccc tataaagagc ctcaggatct
     6301 tgactcagta acttttgaaa aatccgccca ttcaacaaac gaatatgatg ggctagcaaa
     6361 tgactatctt tcataacacc tccaatttat ttcgatgtcg aaatgaataa aataattgta
     6421 acactcatcg ttctaactgt caactatttc gatttggaaa taatttttga taattattca
     6481 caccaccata ctccggctca actaactttt aacgagagtt tctaaattcc ttcgtcctcc
     6541 agtctacaaa agccttccat ccgtactatc ctatttttta tgaggggaca catttttcct
     6601 atcagatcat ttattttaaa gatagaagta aatcataatt gcttccatct gttcttttat
     6661 agtatattga agttggacta ggacactgta tcttctaaaa cattgataaa aagcgacttg
     6721 aatttcccaa tcaatgtgtt cgtatttata gcgttttgaa attggaatag gacgccatga
     6781 ctgctaaaag atttctataa attcatttaa ttcccccaat caatttgttt atatcttatt
     6841 tcatcccgct ataatttcac cttgccctat ctttttcgta gcacccttca aacagcctat
     6901 cccctaccgt ttgacgattc ctcacttcgc tccacttcca ttacagaagt ttcttcacta
     6961 ctatggtctc ggctgacttc tcatgattcc ttgttactac tatttgaacg ctcacgagat
     7021 aggtcttata aaaaatgctt tgatccacaa tggaatcaaa gcattttaaa aagttcctca
     7081 tacataagcg cagaagtcgc agttcctctg tacttggctt cttctctttt gataaagcga
     7141 gccaagttga gcaactcagg tgctggatgt ttgggattta ggagcaattc acgattgacc
     7201 aggcctgaga gacgaactgc ctgcaattgc tcatttgtag taggcagttt tttagtagtc
     7261 tctaggagag cagcaactaa atcttcactc aaatcatgtc gagcatgatt gtaaagatct
     7321 tttataaggc tttctaggtt tggttctacc atccctacca cctcccttat ggtttaataa
     7381 tgtttaatca aatcaaccgt tgaacgatcc aatttcttca ccaaggcttg taagaaagct
     7441 tgcgcttcta ggaagtcatc cattgcatag agggtttggt gagaatggat ataacgagcg
     7501 cagacaccga tagttgttga tgggacacca ccatttttca gatgagctgc acctgcatct
     7561 gttccgcctt taccacagta gtattggtac ttgataccag cttcttcagc cgttgtcaaa
     7621 aggaaatcct tcatccctgg aagaagcaag tgacctggat catagaaacg aatcaaggtt
     7681 ccatctccaa tcttgccttg accaccgtag acatcacctg ctggtgagca atcaactgcg
     7741 aggaagactt ctgggtcaaa cttggttgta gaggtatgag cgccacgcag accaacttct
     7801 tcttggacgt tagaacccag atagagttca ttgccgagtt tttgacctga taaagcttca
     7861 gctagctcgc ttaccatgag gacaccgtag cggttatccc aagcttttga gatgatattt
     7921 ttttcattgg ctgtcaaaat tgcagaacta tctggtacaa tggtatcacc aggacggatg
     7981 ccaaaacttt ctgtctcagc cttgtccgca aaaccaccat caaaaacgat atcggcaatg
     8041 gctggcatgg ttggtccccc ctttccacga gtcaaatgcg gaggaacaga acctgaaatc
     8101 acaggaattt catgaccatc acgagtcaag agtttgaaac gttggccgct aaccaccatg
     8161 gggttccagc caccgatttc tacgacacgg aaggtaccat ctggcttgat ttcgctgacc
     8221 ataaaaccaa cttcgtccat atgagaagcg accaagacgc gcggtgcatc cacagcttct
     8281 gaatgtttga taccaaaaat accacccaag ccatctgtca ccacttcatc cacatgcggt
     8341 gtcaactttt cacgaagata agcacggaca ggcgcttcat gacctgagac tgcagcaagt
     8401 tctgttactt ctttaatttt tgaaaataat gttgtcattt cagttccttc tttctttcat
     8461 ccattttacc actttttata ggagaaggat agtgggaagg tggatttcta agttagtatc
     8521 ttagtcctgc tctatcttag aaaaggatag tattctcttg catgtagtgc aaaatctagt
     8581 aaacattcca aaattaactc gaatatttat ttccaaacaa aaaaacaata caccatcaaa
     8641 gttgtttaga ttttttatga aatttacaga aaataattga cttccctttc ttctttcttt
     8701 aaat
//
LOCUS       DAVGJL010000089         8655 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.090, whole genome shotgun sequence.
ACCESSION   DAVGJL010000089 DAVGJL010000000
VERSION     DAVGJL010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8655
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.090"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            13..1014
                     /locus_tag="VVE42_001564"
     CDS             13..1014
                     /locus_tag="VVE42_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU3073741.1"
                     /translation="MKNINGKKVTIYDIARLSGFSPKTVSRVINGGVNVKEETYQAIQ
                     KVIEELSYIPNAYAKNLTKKEAINILISVKKIDSFPLIWFHTLLDKVLRTCKEFGVNA
                     IVEYFGEEDTISNSIISSTGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSG
                     VVYVSNNNFQATYDMMKAVTEEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDL
                     KVIYGLSTIDSVYSYAMQHLTTQNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISII
                     GFDNIPISQYYTPALSTIAPNYVKLAKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF"
     gene            1622..1699
                     /locus_tag="VVE42_001565"
                     /pseudo
     CDS             1622..1699
                     /locus_tag="VVE42_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            2155..3516
                     /gene="trpE"
                     /locus_tag="VVE42_001566"
     CDS             2155..3516
                     /gene="trpE"
                     /locus_tag="VVE42_001566"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEU3073742.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAY
                     NPVFEIKFENGVLYQNGQVIDRDPLDFLYEVIHKSQHHSELPFGGGAIGFVGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYMVFDHKKEKIHVIEDALYSERSQEALEKSLNQV
                     LEELRIPVPNEFEDLDLSPLDFKPHIAPHKFEVMVETARDLIRNGDMFQCVLSQRFSA
                     EVTGNSFDFYRNLRVTNPSNYLYFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGVTDEEDKTLATDLLSDEKETAEHRMLVDLGRNDIGRISETASVQVTKYMKVELFRY
                     VMHLTSVVKGRLLAELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRGVYAGAI
                     GYLSATGDMDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGE
                     LRP"
     gene            3513..4079
                     /locus_tag="VVE42_001567"
     CDS             3513..4079
                     /locus_tag="VVE42_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEU3073743.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMICDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEEGRAVMRYHSILIEEMPEDFEVTARSTDDQAIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEKVVK"
     gene            4090..5094
                     /gene="trpD"
                     /locus_tag="VVE42_001568"
     CDS             4090..5094
                     /gene="trpD"
                     /locus_tag="VVE42_001568"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEU3073744.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIVTGRVTEAQIASLLLALK
                     MKGETPEERTAIAQVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNMGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEGYAMEDIRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK
                     "
     gene            5091..5858
                     /gene="trpC"
                     /locus_tag="VVE42_001569"
     CDS             5091..5858
                     /gene="trpC"
                     /locus_tag="VVE42_001569"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEU3073745.1"
                     /translation="MSQEFLARILEQKAREVEQMKLEQIQPLRQTYRLAEFLKNHQDC
                     LQVIAEVKKASPSLGDINLDVDIVQQAQTYEENGAVMISVLTDEVFFKGHLDYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNAGATVILLIVAALSEERLKELYDYATELGLEVLV
                     ETHNLAELEVAHRLGAEIIGVNNRNLTTFEVDLQTSVDLAPYFEEGRYYISESAIFTG
                     QDAERLAPYFNGILVGTALMQAENVAQRIKELQIDKG"
     gene            5845..6444
                     /locus_tag="VVE42_001570"
     CDS             5845..6444
                     /locus_tag="VVE42_001570"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEU3073746.1"
                     /translation="MTKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEAAE
                     LAKLIPADVKKVGVFVSPSRVELLEAIDKVGLDLVQVHGQVAADLFENLPCVSIQAVQ
                     VDGNGHVPNSQADYLLFDAPVAGSGQTFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQ
                     HFTPHAVDVSSGVETDGQKDHEKIRRFIERVKHGISGTK"
     gene            6422..7645
                     /gene="trpB"
                     /locus_tag="VVE42_001571"
     CDS             6422..7645
                     /gene="trpB"
                     /locus_tag="VVE42_001571"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEU3073747.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDLTGQNLPDALVACVGGGSNAIGLFHPFVEDESVAMYGAEAAGFGVDTEHHAATL
                     TKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            7638..8414
                     /locus_tag="VVE42_001572"
     CDS             7638..8414
                     /locus_tag="VVE42_001572"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEU3073748.1"
                     /translation="MPKTLTKKLNAIKAAGKGIFVPYIMAGDHEKGLDGLAETIHFLE
                     DLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLADTDIALIPLVSLTTGI
                     ERQKELIEGAEGFVYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     DLERFNAVSDGVIVGSKIVKALHQGEPIQDFIRQAVAYQK"
     gene            complement(8386..8487)
                     /locus_tag="VVE42_001573"
                     /pseudo
     CDS             complement(8386..8487)
                     /locus_tag="VVE42_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
BASE COUNT         2573 a         1610 c         2072 g         2400 t
ORIGIN      
        1 tggaggactg aaatgaaaaa tataaatggg aaaaaggtaa ctatttatga tattgcacgt
       61 ttatcaggtt tttctccaaa aacagtttca cgagttatta atggtggagt aaatgttaaa
      121 gaagaaactt atcaagcgat tcaaaaagtt atagaggaat tgtcgtatat tccaaatgcg
      181 tatgcaaaaa atttaactaa aaaagaagct ataaatatat tgatttctgt aaaaaaaatc
      241 gactcttttc ctttaatttg gtttcatacg ctgttagata aagtgttacg gacgtgtaaa
      301 gaatttggtg ttaatgctat agttgagtac tttggagagg aggatacaat tagtaattca
      361 attatatcaa gcacaggtag cctagtagat ggtgttattg ttttttatga aagtgtagat
      421 gatatcagaa ttcagtattt aaagaagaac catatgcctt ttcttgtttt cggtgaatct
      481 caaacgtctg gagtagtcta tgtatctaat aataattttc aagctactta tgatatgatg
      541 aaagctgtaa ctgaagaaaa atttaaaaat atgtggttgc ttatgggagg agaatcccat
      601 gttaataagg atcgtgaaag aggtgtgcgt tcttttctga atgataaaaa ttactttatg
      661 gatttaaaag tcatttatgg cttatctaca attgattcag tttattctta tgcaatgcag
      721 cacttgacta cccaaaatta tccggatatt atttttgttt caggagatga aaaggttcag
      781 ggcttgattc gtgcgtgcta tgagaaagga attcttattc cagatgatat ttctatcatt
      841 ggatttgata atattcctat ttctcagtat tatactccag ctttgtctac aattgctccc
      901 aattatgtta aattggctaa agaaatgata gaaggtgttt tagcaattat taaaggggaa
      961 agtgtcacat ctgttgaagt ttctcctaaa tttgttagga gacagagttt ctagtattgt
     1021 ttaattcttg aaaatcctag tggaaaagat ggagtgttct aaaataagtt ctgtttagcc
     1081 tccattcagt ttctattgag ttggatccaa tttctaagaa gtgggacatg ttcatgttcg
     1141 atctaaggaa gacatggtca tattcgaatg atattttcaa aaaaatcgta aaaaagtctt
     1201 gacaaagaaa aaacaaagta ttaaaataag ttcaacaaat cagaaaagta actatttttg
     1261 atcttcaggg agcctgtggt gattgtgaat aggtggttgg aagtagtgaa agtgggctga
     1321 ttttgaaaat gaatttgaaa caatgaaaat tcggtgcgca caccttacag tgcagcttgt
     1381 tgttaaacaa ggcagagatg taaagggata gtccctttat aattgaggtg gcaccgcgtt
     1441 accaacgccc tcacacggaa gtatattctg tgtgtgggct tttttctatc cgtcgtttgg
     1501 tttatctttt attagggcct taagtcgctt tgatgaactt gagttctatc tacagctcct
     1561 tgcctactac taaaagcaaa caaaaaggct ggactaatac gaaaaaagct agtaatactc
     1621 aatgaaaatc aaagagcaaa ctaggaaact agccgcaggc tgctcaaaac actgttttga
     1681 ggttgtggat agaactgacg aagtcagtaa ccatacctac ggcaaggcga cgttgacgcg
     1741 gtttgaagag attttcgaag agtataaagg tgtaccgaga tgaacatctc ttcatcaatg
     1801 tctctggtcg tggaggcaag gacctagctg cgattgcaga ttactagaag ctaaaaaata
     1861 atagatggaa aaattacagt cttttctctc acagagagct tgtggttgct gaaaacaagc
     1921 agagaaagct gtaaagttgg gttcatctga aacgagagaa gaaaacatca ttctcaaggg
     1981 agtgcccttt atcgcacagc ctgttacagg aggtatctga gacaggaatg agagatagga
     2041 gaaatcctat aattgaggtg gcaccgcgaa tttcgtcctc acgcaagtta ttttgcgtgg
     2101 ggatttttca tacagtcgcc acgaactaga attagaactg aaagggaaat tacaatggaa
     2161 cgaatcattc atggagatgt cttatcacca atcttggctt atatgcgctt aaaggggcaa
     2221 cataaggtta ttttagagag tattccgaga gacaaggaaa cggctcgttt ttctatccta
     2281 gcctataatc cagtttttga gattaagttt gaaaatggag ttctctatca aaatggtcaa
     2341 gtgattgatc gggatccctt ggatttcctt tatgaagtga ttcataagag tcagcaccac
     2401 tcagagctcc cttttggtgg tggagccatt ggttttgtgg gttacgatat gatttcgctt
     2461 tatgaagaaa ttggtcaaat ccctgaggat acaattggga cgccagacat gcatttcttt
     2521 gtctatgaga gctatatggt ctttgaccac aagaaggaga agatacatgt catagaggat
     2581 gcactttata gtgagcgcag tcaagaggcc ttggaaaaat cattgaacca agtgcttgag
     2641 gaattacgca ttcctgttcc aaatgaattt gaagacttgg atctatctcc gttagacttc
     2701 aaaccgcata tcgctccaca taagtttgag gtaatggtag aaacagctcg tgacttgatt
     2761 cgtaatggtg atatgttcca atgtgtactc agccagcgtt tctcagcaga agttactgga
     2821 aattcatttg acttctacag aaatctccgc gtgaccaatc catccaatta cctctatttc
     2881 tatgattttg gggattatca aatcatcgga gctagtccag aaagtttagt ttctgtcaaa
     2941 aatggcatcg tgacaaccaa tccgattgca gggacgcgac caagaggggt tacggatgaa
     3001 gaagacaaga ccttggcgac tgacctcctc tctgatgaga aggaaacagc agaacatcga
     3061 atgttggtag acttggggcg taacgatatt ggccgcatct ctgaaacggc cagtgtccaa
     3121 gttactaagt atatgaaggt ggagctcttc cgctacgtca tgcatttgac cagcgtggtc
     3181 aaggggcgtt tgcttgcaga actcacggcc atggatgcct tgaaagctac acttcctgct
     3241 ggaaccgttt ctggagcacc aaagattcgg gcgatgagac gcatctatga actggaaacg
     3301 gaaaaacggg gcgtatatgc aggagcaatc ggctacttgt ctgcgacggg tgatatggat
     3361 ttggccattg ccatccgaac catgattctc aaaaatcaaa gagcctatgt gcaggctggg
     3421 gcagggattg tctacgactc tatcgcccaa aacgaatacc aagaaaccat taacaaagca
     3481 aaatctatga ctagaattgg agaactaaga ccatgatttt attgattgac aactatgatt
     3541 cttttaccta taacttggcc caatacattg ggaattttgc agaagttcag gttctgagaa
     3601 atgatgattc caagctgtat gaagaagctg aaaaagcaga tggtctggtc ttttctcctg
     3661 gtcctggttg gccagttgat gctggaaaga tggaagacat gatttgtgat tttgcaggca
     3721 agaagccgat tctagggatt tgtttgggcc accaagccat tgcagaagtc tttggtggta
     3781 agctaggttt ggctccaaaa gtcatgcatg ggaaacagag caatatcaac tttgaagcgc
     3841 catctgtttt gtatcaaggt attgaggaag gacgtgcggt catgcgttat cacagtattt
     3901 tgattgagga aatgccagaa gactttgaag tgacagctcg ttcgactgat gaccaagcca
     3961 tcatggggat tcaacataaa aacctaccga tttatggctt ccagtaccat ccagagagca
     4021 ttggaacgcc agacggcttg tcttctattc ggaattttat cgagaaggtt gtaaagtgag
     4081 gaaactagga tgaaagagat tattgaaaaa ctagcaaaat ttgaaaattt atcaggtgtg
     4141 gaaatgacgg atgtcattga gcgtatcgta actgggcgtg taactgaagc gcagattgct
     4201 tctctcctct tagctcttaa gatgaagggg gaaacacctg aagaacgcac agccattgcc
     4261 caagtcatga gaggacatgc ccagcatatt ccaactgaga ttcatgatgc catggacaac
     4321 tgtggtacag gtggggacaa gtctttcagt tttaatattt ccacaactgc agcctttgtc
     4381 ttggctggtg gcggtattca catggccaag catggtaacc gctcgatttc ttctaaatct
     4441 ggttccgcag atgtcctcga agccttggga atcaatcttg acctcaaacc agctgaacta
     4501 ggtaaggtct ttgataaaac tggaatcgtc tttctcttcg ctaaaaatat gcacccagct
     4561 atgaaataca tcatgccagc tcgtttggaa ttgggaattc caacgattat gaacttgact
     4621 ggtccactga ttcatccaat ggctttagaa acacagcttc ttggaattag tcgtccagaa
     4681 ctcctagaaa gtacagctca ggttttgaaa aatatgggtc gcaaacgtgc catcgtggtt
     4741 gctggaccag aagggttgga tgaagctggc ttgaacggaa caaccaagat tgcacttctt
     4801 gaaaatggcg aaatcagctt gtcaagcttt actccagagg atttgggaat ggaaggctat
     4861 gctatggaag atattcgtgg tgggaatgct caggaaaatg cagaaatttt gcttagcgtt
     4921 ctgaaaaacg aagcaagtcc attcttggaa acgacagtct tgaatgctgg tcttggtttc
     4981 tatgctaatg gtaagattga tagcatcaag gaaggagttg ccttggcccg tcaagtgatt
     5041 gctagaggca aggcccttga aaaactcaga ctgttacagg agtaccaaaa atgagtcagg
     5101 aatttttagc acgaatctta gagcagaagg cgcgtgaggt ggagcagatg aagctggagc
     5161 aaatccagcc tctgcgccag acctatcgct tggcagaatt tttgaagaat catcaggact
     5221 gcttgcaggt aatcgctgag gtcaagaaag ctagccctag tttgggagat atcaatctcg
     5281 atgtggatat tgtgcaacag gcccagactt atgaagaaaa cggagcagtg atgatttcgg
     5341 tgttgacaga tgaggttttc tttaaagggc atttggatta tctacgggaa atttccagtc
     5401 aggtagagat tccgacgctc aacaaagact ttatcataga tgaaaagcaa atcatccgcg
     5461 ctcgcaatgc aggtgcgaca gttatcttgc ttattgtggc agccttgtcc gaagaacgcc
     5521 tcaaggaact gtatgactac gcgacagagc ttggtctgga agtcttagtg gagactcaca
     5581 atctagctga actagaggta gcccacagac ttggtgctga gattatcggg gtcaacaacc
     5641 gcaacttgac tacctttgag gtcgacttgc agaccagtgt agatttagcc ccttactttg
     5701 aggaaggtcg ctattacatt tctgaatctg ccattttcac agggcaggat gcggaacgac
     5761 tagccccata ctttaacgga attttggtag ggacagctct tatgcaggca gaaaatgtgg
     5821 cccagagaat caaggagttg cagattgaca aaggttaaaa tttgtggact atcgaccaaa
     5881 gaagcggtgg aaacagccgt ttcagcagga gccgactata tcggttttgt ctttgcacct
     5941 agtaaaagac aggtgacttt agaagaggca gctgagttgg caaagcttat tcctgcagat
     6001 gtgaaaaagg ttggagtatt tgtttcacca agtcgggtag aactgctgga agcgattgac
     6061 aaagttggct tggacttggt tcaagttcac ggtcaggtgg cagctgattt atttgagaat
     6121 ttgccttgtg tcagtattca ggctgtgcag gtagatggaa atgggcatgt ccccaattct
     6181 caggcagact atctactctt tgatgcccca gtggcaggga gtggccagac ctttgactgg
     6241 ggtcaactgg atacgactgg actagcacag cccttcttta tcgcaggtgg ccttaatgaa
     6301 gataatgtag taaaagcaat tcaacatttt actccccatg cagtagatgt atcgagcgga
     6361 gtggagacag atggacaaaa agatcatgaa aagattagaa gatttataga gagggtaaag
     6421 catggcatat caggaaccaa ataaagatgg attttacgga aaattcggcg gacgttttgt
     6481 cccagaaaca ttgatgacag cagttttgga gttggagaag gcctaccgtg aaagtcaggc
     6541 agacccaagt ttccaagagg aattaaacca gcttttgcgc cagtacgtag gacgtgaaac
     6601 tcctctttat tacgcaaaaa acttgaccca gcatatcggc ggagccaaga tttatctcaa
     6661 acgggaagac cttaaccata caggagccca caagattaac aatgccttgg gacaagtttg
     6721 gcttgccaaa cgcatgggta aaaagaaaat tatcgcagaa actggtgctg gtcagcacgg
     6781 tgtggcaact gcaactgctg cggccctctt taacatggaa tgtaccatct acatgggtga
     6841 ggaagatgtc aaacgccaag ccctcaatgt cttccgtatg gagcttttgg gagctaaggt
     6901 cgaggccgtg acagatggtt cgcgcgtgct caaggatgcg gtcaatgcag cccttcgttc
     6961 atgggtggct aatatagatg atacccacta tatccttggt tctgccttgg ggcctcatcc
     7021 atttccagaa attgttcgtg acttccaaag tgtcattggt cgagaggcta aacaacagta
     7081 ccgtgacttg acaggacaaa atctgccaga tgccctagta gcctgtgttg gtggtgggtc
     7141 gaatgccatc ggtctcttcc atccctttgt agaagatgag tcagtagcca tgtatggagc
     7201 tgaagcggct ggatttggtg tggatacgga gcaccacgca gctaccttga ccaagggtcg
     7261 tccaggtgtc cttcacggtt ccctcatgga tgtgctccaa gatgcccatg gtcaaattct
     7321 tgaagccttc tctatctcag caggtttgga ctatcctggt atcggtccag agcattctca
     7381 ctaccacgat attaaacgtg ccagctatgt ccctgtgaca gacgaagaag ccttggaagg
     7441 attccaactc ttgtctcgtg tggaagggat tatcccagct ttggaatcta gccatgctat
     7501 cgcctttgcg gtgaaattgg ccaaagaact tggaccagaa aagtctatga ttgtatgcct
     7561 atcaggtcgt ggggacaagg atgtggttca agtcaaagac cgcttggaag cagatgcagc
     7621 aaagaaggga gaagctcatg cctaagacac taacaaaaaa attaaatgct attaaagcag
     7681 ctggaaaagg aatttttgtt ccctatatca tggctggtga ccacgaaaaa ggtttggatg
     7741 gtcttgctga aacaattcac tttttagaag atttgggtgt ctctgcaatt gaagtgggta
     7801 ttcccttttc agaccctgtt gcagatggac ctgttatcga agaagctggc ttgcgcagtt
     7861 tagcccacgg gacctctacc caggctttag ttgaaacctt gaaaaccatt gaaacagaga
     7921 ttccactggt catcatgacc tacttcaacc ccctctttca gtacggtgtg gagaactttg
     7981 tcaaagatct ggctgataca gctgtgaagg gcttgattat cccagacctg cctcatgaac
     8041 atgccaactt tgtagaacca tttttggcag atacagatat cgccttgatt cctttagtaa
     8101 gcttgaccac aggaattgag cgccagaaag agttgattga aggcgcggag ggattcgtct
     8161 atgccgttgc catcaatggg gtgacaggga aatcaggcaa ttaccgtgca gatttggaca
     8221 agcacttggc gcaacttcat caagtggccg acatcccagt cttgacaggt tttggtgtat
     8281 ctagtcaagc cgatctggaa cgcttcaatg cggtgtcaga tggtgttatc gtcggttcga
     8341 aaatagtaaa agctctccac caaggagagc cgattcagga ttttatcaga caagcagtag
     8401 cttaccaaaa ataatcaaac aagtagcaag taagaggaaa gacacgaaag gaagtctttc
     8461 ctttttcttt tgcaggagaa aggctagttt tatagtgaat tgaatctgga atattacact
     8521 acggttccta aaatatttta agaaagcgat ttgaatttcc caataaattt gttcagtttt
     8581 taattgattt tactatagaa gatataattt tctaaattac tctgacacag aactttaaga
     8641 aaaagctaat tattg
//
LOCUS       DAVGJL010000090         8631 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.091, whole genome shotgun sequence.
ACCESSION   DAVGJL010000090 DAVGJL010000000
VERSION     DAVGJL010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8631
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.091"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            47..397
                     /locus_tag="VVE42_001574"
     CDS             47..397
                     /locus_tag="VVE42_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEU3073749.1"
                     /translation="MLRHPGISPTNDLIAKKIFSNPEITCQFIRDMLDLPAKNVTILE
                     GSDIHVLLSMPYSVQDFYTSIDVLAELDNGTQVIIEIQVHHQNFFINHLWAYLCSQVN
                     QNLEKIRQREGDTH"
     gene            612..728
                     /locus_tag="VVE42_001575"
     CDS             612..728
                     /locus_tag="VVE42_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEU3073750.1"
                     /translation="MVGVFRQQALYPATATSHYPSKSTAGLQELVRGGQENV"
     gene            721..954
                     /locus_tag="VVE42_001576"
     CDS             721..954
                     /locus_tag="VVE42_001576"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073751.1"
                     /translation="MFSQLHMREEQVLLAQDYALETARAEGLEQGLERGKVEGRAERK
                     LFAFLDIVRQGLLTSEVASQQLGMSVSEFEALL"
     gene            1080..1361
                     /locus_tag="VVE42_001577"
                     /pseudo
     CDS             1080..1361
                     /locus_tag="VVE42_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1495..2463)
                     /locus_tag="VVE42_001578"
     CDS             complement(1495..2463)
                     /locus_tag="VVE42_001578"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEU3073752.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALAEAGAVEVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
     gene            complement(2608..3231)
                     /locus_tag="VVE42_001579"
     CDS             complement(2608..3231)
                     /locus_tag="VVE42_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HEU3073753.1"
                     /translation="MPKKVLILSLLGGMFLSGWLSSFANTYIHDLLGVLFPDSTFLNA
                     FESAIVAPLVEEPLKLLPLVFVLALIPVRKLKSLFLLGIASGLGFQMIEDIGYIRTDL
                     PEGFDFTISRILERIISGIASHWTFSGLAVVGVYLLYRAYKGQKVGKKQGLIFLGLAL
                     GTHFLFNSPFVELETELPLAIPVVTAIALYGFYHAYCFVEKHNELMT"
     gene            complement(3213..3422)
                     /locus_tag="VVE42_001580"
     CDS             complement(3213..3422)
                     /locus_tag="VVE42_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073754.1"
                     /translation="MKKSSILFIFILLLCIGLQYETIYYTDGSRSGAEYGLMGVSIFL
                     ALFYMIPALYFLFRIGKNGNCQRRF"
     gene            complement(3447..3944)
                     /locus_tag="VVE42_001581"
     CDS             complement(3447..3944)
                     /locus_tag="VVE42_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEU3073755.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESPLIPDDVIISGAIYNVDTGSM
                     TVVEL"
     gene            complement(4017..5378)
                     /gene="radA"
                     /locus_tag="VVE42_001582"
     CDS             complement(4017..5378)
                     /gene="radA"
                     /locus_tag="VVE42_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEU3073756.1"
                     /translation="MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
                     RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKK
                     VFA"
     gene            complement(5392..5907)
                     /locus_tag="VVE42_001583"
     CDS             complement(5392..5907)
                     /locus_tag="VVE42_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU3073757.1"
                     /translation="MKIIFVRHGEPDYRELEERSYIGFGIDLAPLSEMGRQQVQKLSK
                     NPLLSSAEIIVSSAVTRALETASYVVCATGLPLRVEPLLHEWQVYKTGIENFETARRL
                     FLENKGELLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            complement(5909..6352)
                     /locus_tag="VVE42_001584"
     CDS             complement(5909..6352)
                     /locus_tag="VVE42_001584"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEU3073758.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAAGGVRTGGFGSTGH"
     gene            complement(6412..6505)
                     /gene="ffs"
                     /locus_tag="VVE42_001585"
     ncRNA           complement(6412..6505)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VVE42_001585"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(6538..7005)
                     /locus_tag="VVE42_001586"
     CDS             complement(6538..7005)
                     /locus_tag="VVE42_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEU3073759.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGAAGSLYNILTDERLNHRVEVEIGILEDECAAIMQDFFRNRRKK"
     gene            complement(7206..8492)
                     /locus_tag="VVE42_001587"
     CDS             complement(7206..8492)
                     /locus_tag="VVE42_001587"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEU3073760.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGVRNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
BASE COUNT         2713 a         2085 c         1478 g         2355 t
ORIGIN      
        1 ttattcacat cttatttcaa ctcactatag aaggaggaat aggaggattc tcagacatcc
       61 gggcatcagc ccaactaatg atttgattgc taagaaaata ttcagcaatc cagaaatcac
      121 ttgtcaattt attcgcgata tgctggactt gccagcaaaa aatgtgacca ttttggaggg
      181 aagcgatatt cacgtattac tctccatgcc ttactcggtg caggattttt ataccagtat
      241 agacgtcttg gcggagttgg ataacggtac tcaagtaatt attgagattc aagtccatca
      301 tcagaatttt ttcatcaatc acttgtgggc ttacctgtgc agtcaggtta atcaaaatct
      361 tgaaaaaatt cgtcagcgag aaggtgatac tcactagatc tacaaacaca tcgctcctgt
      421 ttacgtcatt gctatcgtgg atagtaatta tttctcagat gacctggctt ttcatagctt
      481 tagtatgcgc gaagacacaa caggtgaggt attggcgatt accaacaatg gacaggaaaa
      541 ccatctggtt aagatggcat tcttggaatt aaaaaataca gagaaaccag caaagacaag
      601 gttcgcaagc catggttgga gtttttcggc aacaagccct ttacccagca accgcaacga
      661 gccattaccc aagcaaatca actgctggac tacaagagct ggtccgagga ggacaggaaa
      721 atgtttagtc aactacatat gcgagaagaa caagtcttgt tagcacagga ctatgccttg
      781 gaaactgcta gggctgaagg ccttgaacaa ggactagagc gtgggaaagt tgaaggaagg
      841 gcagaaagga aactttttgc cttcctagac atagtacgcc aaggtcttct gacttctgag
      901 gttgccagcc agcaattagg tatgtcagta tctgaatttg aggcactgtt gtaaaatggc
      961 tccataatat ccatagggga tttacccact atggatatta tggagcctat tttgtgtaaa
     1021 aaaaagtccc atatgaccta taatgaaaag cgacaaaaca actcattaga aagaatcata
     1081 tggaacaatt acattttatc acaaaattac tagacattaa agaccctaat gtccagattt
     1141 taaacatcat caataaggat acacacaagg aaatcatcgc caaactggac tacgacgccc
     1201 catcttgccc tgagtgcgga aaccaattga agaaatatga ctttcaaaaa ccttctaaaa
     1261 ttccttatct tgaaacgact ggtataccta ctagaattct ccttagaaag cgtcgattca
     1321 agtgctatca ctgttcaaaa atgatggtcg ctgaaacttc tgatgacgta cagtcatatt
     1381 tcttctcttt ttattatatc acagttttaa atctagcttt actagattca ccgctactat
     1441 ctatttattc ggaaaaaaga cgaaaaaacc tgagaatcat ctcaggcttg gtcattaaat
     1501 ttttttctca atatcgaaaa gtggagaaag tggtcgtttt tcatgaatac gtacgatagc
     1561 atcccctagg agatgagcga ttgaaatctg ctcaatctta tcaatcaaac gctcttctgg
     1621 cagatagatg gtatccaaaa caaccaattt cttaatagct gatttttgga tattgtccgt
     1681 agcaggacca gaaagaactg ggtgcgtaca gcttgcatag acttcaacag caccagcttc
     1741 cgcaagagca tctgccgcat gacaaatcgt tccagcggta tcaatcatat catcaatcaa
     1801 gatacaagtc ttgccttcaa ccttaccgat gatgttcata acttcactag tattcatctt
     1861 atcaacgcta cgacgtttat caataatagc gatagatgtt ttcaaaaatt ctgccaactt
     1921 acgagcacga gtcacccctc catggtccgg gctgacaacc acatagtcag aaccaaccat
     1981 accacgacgc tcaaaataat ctgcaatcag aggagcaccc atcaaatgat ccacaggaat
     2041 atcaaagaat ccttgaattt gcgcagcatg caagtcgatg gtcaataaac gatccactcc
     2101 agctacttca agcatatttg cgacaagttt tgaagtgatt ggctcacgcg ctctcgcctt
     2161 tctatcctga cgtgcatacc catagtaagg catgacaaca ttaacagatt ctgcactcgc
     2221 acgcttcaaa gcatctacca taatcaaaat ttcaagcaga ttgtcattta caggcgaact
     2281 agttgattgt aagataaaga cgtgtttccc acggattgat tcttcaatgt tgacctgaat
     2341 ctctccatct gaaaattggc gaacacttga tttccccaac tctatcccaa tctcctgcgc
     2401 cacacgttct gccaattctt tattagaaga aagggcaaac agctttaaat cagaaaaaga
     2461 catgatttcc tccggtatat atgtataact tgtgcttttc acaagatttt ccatctacca
     2521 ttgtagcgct ttttgcacta tttttcaatc aaaaataaaa gaagggcacc atatttgtac
     2581 ccttgcatca ttcttttgaa aaatattcta ggtcatcaac tcattgtgtt tctcaacaaa
     2641 gcaataagca tgataaaaac catagagagc aatagccgta accactggaa tcgctaaagg
     2701 caactctgtt tccaactcca caaaaggaga gttaaacaag aagtgagttc ccaaggctaa
     2761 acctagaaaa ataagtccct gtttcttgcc aaccttctgt cctttatagg ctctgtaaag
     2821 caagtaaaca cctactacag ctagacctga aaaagtccag tgagaggcaa ttcctgagat
     2881 gatacgctct aaaattcgcg aaatagtaaa gtcaaagccc tctggcaaat ccgtacgaat
     2941 ataaccaata tcctcaatca tttggaatcc caaaccggaa gcaattccaa gtaaaaacaa
     3001 agattttaat tttcgcacag gaatcaaagc caaaacaaaa acaagtggca ataatttcaa
     3061 gggttcttct accaaaggag ccacaatagc actttcaaag gcatttaaaa atgtactatc
     3121 tgggaaaaga acccccagta aatcatggat ataagtatta gcaaaactag acaaccagcc
     3181 tgaaaggaac atccctccca ataaagacag aatcaaaacc ttctttggca attcccattt
     3241 ttcccaatac ggaagagaaa ataaagagcc ggaatcatgt aaaagagagc tagaaagata
     3301 gaaactccca ttagtccata ttccgcacct gacctcgaac cgtccgtata gtagatggtt
     3361 tcatactgta aaccaataca tagcaataaa ataaaaataa ataaaatact gcttttcttc
     3421 atacactttc tttctaaatg aagtatttat aattctacga ctgtcatact tcctgtatca
     3481 acattgtaaa tggcaccaga gataatgaca tcgtctggta ttaggggaga ctcgataagc
     3541 agttgcatat cctcgcgtac actctcttct atatcttgga agggcaagaa gtcctggtct
     3601 gacacatcta cacctaattc ctcttttaaa tactcctgaa aaggttcatt ttcaaaggtc
     3661 tgagcaccac agtctgtatg gtgcaatacc acaatctctc ttgtccccat ttgttgctgg
     3721 gaaataacta gcgaacgaat catgtcttca gtcactcgac cacctgcatt ccgcaagata
     3781 tgagcatccc caagtgccaa acctagagct tgcgcaacgt gcaaacgtga gtccatacag
     3841 gtcacaatgg ctactctggt tttaggttta agtggcagat ttaactgccc atgtagggca
     3901 acataagcct gattggcttg cataaactgt tcaaaatacg acacgattcc ctccttgaaa
     3961 atttgatagt caaatatttc tcctatctta tcatttttaa gagaatttgt cacggattat
     4021 gcaaagacct ttttcaagac ttcctgaatc gttgtcacgc caatgacctg aatttcctta
     4081 ggcagagtga ttcctgtcaa ggaattctta ggtacataaa tcttagtaaa gcccagttta
     4141 gcagcttcgt tgatgcgttg ctcaatacga ttcacgcgcc gaatctctcc tgtcaagccc
     4201 agttctccga caaaacattc ctgaggatta gttggcttgt ctttgtagct cgaagcaata
     4261 gcaactgcaa cagccaagtc aatggcaggt tcatccaatt taacaccacc agcagatttg
     4321 agataggcat cctgattttg caagagaagc cctgcccgtt tttccaaaac agccataatc
     4381 aagctagcac ggttaaaatc aagtcctgtc gtagtacgct tggcatttcc aaacatggtc
     4441 ggtgttacca aagcctgaac ctccgccaaa atcggacgcg tcccttccat ggttacaacg
     4501 atggaggaac cagtcgcccc atccaaacgc tcttctagga aaacttgact cggattgagt
     4561 acctcaacca agccgcccga ctgcatctca aaaatcccaa tctcattagt ggaaccaaaa
     4621 cgatttttga ccgctctcaa aatacgaaag gtgtggtgac gctccccttc aaagtaaagc
     4681 accgtatcca ccatatgctc caacatacga ggcccagcca aggttccttc tttggtcaca
     4741 tgacctacga taaagatggc aatgttattg gtcttggcca actgcatgag ttcagcggtc
     4801 acttcacgta cctgagaaac agacccctgc acccctgaaa tctcaggaga catgatggtc
     4861 tggatggaat caataatgag aaagtctggc tggatacgct ccacttctgc acgaacactc
     4921 tgcatattgg tctctgcata gagataaaac tcactatcaa tatcacctaa gcgctctgca
     4981 cgtagtttaa tctgctgggc agactcctcc ccactgacat agagaactgt ccccacttgg
     5041 gacaactggg ttgagacttg taggagaaga gttgatttcc caatcccagg atccccaccg
     5101 atgaggacga gacttcctgg taccactccg cctccaagca cacggttgaa ttcctccatc
     5161 tccgtcttgg ttcgattgac attgatggaa gtcacctcag ctagtttcat gggcttggtt
     5221 ttctcacctg tcaaggacac acgcgcattc ttgacctcgg caacctcaac ctcttccaca
     5281 aaagaagacc aagacccaca gttggggcaa cgtcccagat atttagggga attataccca
     5341 caattttgac atacaaatgt cgcttttttc tttgcgatga caaacctctt tctatatctc
     5401 taactcacac tcaatcactt ggcaaaaatc aatcttctca tttggcacaa actggcgcat
     5461 gagcattcga tgagcaacaa ctaccacagt ctgatgttct cgatacttag acatacattc
     5521 tagaaaccga gacttcattt ccgtagctgt ctcatattga ataggactat taggaagcaa
     5581 ctcccccttg ttttctaaaa acagtcttct agctgtttca aagttttcta ttcctgtttt
     5641 atagacctgc cattcatgta ataaaggctc tactcttaaa ggaagacccg tagcacagac
     5701 cacatacgaa gccgtttcta aagctcttgt gactgcagaa gatacgatta tttcagctga
     5761 cgagagtaaa ggatttttgc tcaatttctg gacttgctgc cgtcccatct cagacaaggg
     5821 tgccaaatct atcccaaatc ctatataaga acgctcctct aactcacggt aatctggctc
     5881 cccatgacgt acaaagataa tcttcattct agtgccctgt cgatccaaat ccaccagttc
     5941 gaacgccacc agctgcatct ccatctgcaa ttaagaaagt agcaaaaaca gcctggacaa
     6001 tacgttcccc aacttcaaga acaacctctt ggtctgtgat attcttcatc tgcgcaaaaa
     6061 tatgcccttc atttccagga tttccataat aatccccatc aatgacccca actgagttaa
     6121 ttaaaaccaa gcccttctta cgaggatttg aagaacgatc atagaggtag agaacctcag
     6181 tcggctgcat ataagcctta acccctgtcg gaaccaagac aatctctcct ggcgcaacaa
     6241 ctgtacgcac agcaaccttt aagtcgtaac cagccgcatg cgctgtctca cgcttgggca
     6301 ataaattttc atctgtaaaa cttgaaacca attcaaaacc acgaattttc ataattttct
     6361 cttttctatt atcatttatt ctagattatt ctatcttatt tattcggaaa aagcacgaaa
     6421 aaagagcaca caattcaaat cgcttagggc tgctggattc ctcccctgac ccgcttcacg
     6481 cagaactgtt gctccactat ttattatatc acattcctat tcattttaaa agcaaaatta
     6541 ttttttccgt ctatttctaa aaaaatcctg catgatagct gcgcattcat cttccaaaat
     6601 tcctatttca acctccacac gatgattgag acgctcatct gtcaagatat tgtacaaact
     6661 cccagcagcg ccaaatttct ggtttttagc cccatagacc acatttggaa tgcgggcaag
     6721 cccaatcgct ccactacaca tgacacaagg ttcaatggtc acaaaaagtg tgcaatccag
     6781 caagcgccag ctctcctcac tcaagttcgc atcctctata gccataattt ccgcatgcat
     6841 aaccgctcgc tgtaattcct cacgcgcatt atgcccacga ccaatgattt ccccatcttt
     6901 gacaatcaca caaccaattg gaatttcatc gtgttcaaga gcaatctcag cctctctcaa
     6961 agcctccctc ataaagactt ctttttcttc aagcgtataa tacatctctt ttctcttttc
     7021 ctacttatcg attttattat tatatcatga atcccaacac aaaaaaagcc accgaatgcg
     7081 gtgactctat agggagatta ttatgaaaaa gaaaagttta ggatatttgt tacaacaagt
     7141 taggaggtct tcttgtaacc gtctatagta tacccgacct atcttaaaca aatcttaaaa
     7201 atctcttagg accaaacact ttctaaaata tttgtttgtt cacgaccagg acctactgag
     7261 aaagtagaaa tacgaacgcc aaccaattca ctcacacgac gaacatagtt acgcgcattc
     7321 tcaggaagat cttccaaatt gcgaactccg gtaatatctt ctgaccaacc tggcaactct
     7381 tcatagatag gcttgcaacg tttcaattgc tcaagactag ctggatagta gtcaatacgt
     7441 tgaccgtcaa gatcataggc cacacagatt ttcacagtat ccaaaccgct caaaacatca
     7501 atagagttca aagaaaggtt agtaatacca gaaacacgac ggctatgacg catcacaact
     7561 gagtcaaacc aacctacacg acgtggacga ccagttgttg taccatactc atgacccact
     7621 tcacggatac gttctcccac ttcatcaaac aactcagttg ggaaaggacc atctcctaca
     7681 cgactcgtat aagctttaca tacacctaca accttgtcaa tcttgcttgg accgacacca
     7741 gaaccaattg tcacaccacc agctacaggg tttgatgacg taacaaatgg ataagtacct
     7801 tggtcgatat ctagcataac accttgtgca ccttcaaaaa gcacacgttt gccattatca
     7861 agcgcatcat tcaagataac agatgtatct atcacgtatt tcttgatttg ttgaccatat
     7921 tcgtaatatt cttcaaaaat atcatcgaaa acaatcgctt tactgtcata caatttttca
     7981 aaaagacgat tcttttcagc aaggttacgt tctaaacgct cacggaaaat atctttatct
     8041 aaaagatctg caatacgaat tccaacacga gcagccttgt ccatataagc tggaccaatt
     8101 cccttaattg tcgtaccaat cttattgtcg cccttagctt cttcttgcaa gcgatccaac
     8161 tcgatatgat aaggcaaaat aacatgcgca cgatcagaaa tacgcaagtt atcagttgtt
     8221 acaccttcct catgaagata gctcaactct tttacaagag atttaggatt tacaaccata
     8281 ccattcccaa tgacagatat tttttcaggg aagaaaatcc cagatggaat caagtgcaac
     8341 ttaaatttct taccgtcaat cacaatcgtg tgaccagcat tatcaccacc ttggtaacgt
     8401 gcaatcactt ctgcattcgc tgaaaggaag tctgtaatct tccctttacc ttcatcaccc
     8461 cattgggtac ctacaacaac aactgaagtc ataatcttgt ctgagccctc aggctcttcc
     8521 tttctcacat acatggcagg actctcacct gcaattatat cttacaattt attataataa
     8581 aaaatcgcct ttttatcaag aagaaacaat agaaagattt gctatttcca a
//
LOCUS       DAVGJL010000091         8491 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.092, whole genome shotgun sequence.
ACCESSION   DAVGJL010000091 DAVGJL010000000
VERSION     DAVGJL010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8491
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.092"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(95..748)
                     /gene="phoU"
                     /locus_tag="VVE42_001588"
     CDS             complement(95..748)
                     /gene="phoU"
                     /locus_tag="VVE42_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEU3073761.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
     gene            complement(760..1518)
                     /gene="pstB"
                     /locus_tag="VVE42_001589"
     CDS             complement(760..1518)
                     /gene="pstB"
                     /locus_tag="VVE42_001589"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU3073762.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLDPQHKETEDYITGKFG"
     gene            complement(1531..2334)
                     /gene="pstB"
                     /locus_tag="VVE42_001590"
     CDS             complement(1531..2334)
                     /gene="pstB"
                     /locus_tag="VVE42_001590"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU3073763.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            complement(2345..3229)
                     /gene="pstA"
                     /locus_tag="VVE42_001591"
     CDS             complement(2345..3229)
                     /gene="pstA"
                     /locus_tag="VVE42_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEU3073764.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVVHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGSYLHKKLTAA"
     gene            complement(3219..4136)
                     /gene="pstC"
                     /locus_tag="VVE42_001592"
     CDS             complement(3219..4136)
                     /gene="pstC"
                     /locus_tag="VVE42_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEU3073765.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGTWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            complement(4136..5014)
                     /gene="pstS"
                     /locus_tag="VVE42_001593"
     CDS             complement(4136..5014)
                     /gene="pstS"
                     /locus_tag="VVE42_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEU3073766.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            complement(5588..6892)
                     /locus_tag="VVE42_001594"
     CDS             complement(5588..6892)
                     /locus_tag="VVE42_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEU3073767.1"
                     /translation="MQFPEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
                     YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGRLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQIFGDQLYLLPEL
                     LPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKPSQVEQSVEIGQEAFVKYAAGET
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            complement(6893..7666)
                     /locus_tag="VVE42_001595"
     CDS             complement(6893..7666)
                     /locus_tag="VVE42_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEU3073768.1"
                     /translation="MESKFEFAKELVKKAGQYILDHMQEDLRVETKSSPTDLVTRLDK
                     EVQELLVGEILSRYPEDKICAEEGCLRASVQDGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNDRPLAPFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRAVLGVRVYGSAAISFAKILSGRLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            complement(7656..7934)
                     /locus_tag="VVE42_001596"
     CDS             complement(7656..7934)
                     /locus_tag="VVE42_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEU3073769.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGEGKISLGK"
     gene            complement(7934..8335)
                     /locus_tag="VVE42_001597"
     CDS             complement(7934..8335)
                     /locus_tag="VVE42_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEU3073770.1"
                     /translation="MITLFLSPSCTSCRKAKAWLEKHKVPFVEHNIMTSPLTRKELQH
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
BASE COUNT         2530 a         1992 c         1586 g         2383 t
ORIGIN      
        1 gttttttaaa tttttatctc aaaaatattt tttcgttcaa aaaaaggagc catcagttga
       61 tttcaagctc ctttttatac agaattaaac tattttatag ttcgacaatc ttacctgttt
      121 caaagtagac aacccattca cagatatttt tagcatagtc accgatacgc tccaagtagg
      181 aaataacttg gaaataatca cgacccgtaa caatggcttc tggatttttc ttaatctctt
      241 cagtcgcaag gtcacggata gtttcaaaat agtggttaat ttgctcatcc atggaggcca
      301 cccggtatgc gtcgtcaaca gaaccattaa gataaagatc aagtgctgct tcaacaacgc
      361 ttttaacttc acgtcccatt tttttaattt cttcctctac agctggaata cgttcttccc
      421 ccttcatacg gatggttgcc tgggcaatgg ctacagcgtg atcccccata cgctccacat
      481 ctgatacagc cttaaggaca gtcaagactg tacgcaaatc ttgagagact ggttgttgga
      541 gtgcgatcat ttcaaatgat ttcttttcca gtttcacttc gtattcattt acttctgcat
      601 catcttcgat gacctctttt gccaggtcac ggtcatgcgt gacaaaagca cgtaccgtac
      661 gattgatttg tgagagcact tcttgtccca tagcgtagaa ctggttatgt aatttctcta
      721 aatcttcttc aaattgagat cgtaacatct ttcatctcct tatccaaatt ttcctgtaat
      781 atagtcttcc gtttccttgt gttggggatc aaggaacatc tgcttggtat cattaaattc
      841 aatcaaatct ccatctagga aaaatcctgt cttatcagag atacgtgaag cttgctgcat
      901 ggaacgggtt accagaagca tggtgtactt gtcttttaga ccatacaagg tttcctcaat
      961 tttaccagct gaaatcggat ccaaagccga agttggctca tccaagagga tgattttagg
     1021 actagttgcc aagacacggg ccacgcagac acgctgctgt tgaccacctg acaatccaat
     1081 agctgaatca tatagacgat ccttgacctc atcccagata gaggcacctt gcaaggcttt
     1141 ttctacggct tcatccagaa cctgcttatc cttaattcca ttgatacgaa gcccgtagac
     1201 aacattctca tagatagtca tagggaaagg attaggttgt tggaaaacca ttccgatttc
     1261 cttacgtaat tcaaccgtat ctgtacgcgg actgtagatg ttgtgaccat tgtacaccac
     1321 ggatccagtt gtggtcacct ctggattgag atctcccatg cggttgagag acttgaggag
     1381 ggttgacttc cctgatccag atggaccaat caaggctgta atttccttag gttggaaaga
     1441 tagggaaaca ctattcaaag ccttcttttt attataataa acggacaggt ctgatacctg
     1501 taaaatcgca tctgtcatac ggtttccttt ctaaccaaag tgaccagata catagtcatt
     1561 ggtggactgt agcttggcat tttggaaaat agttgcagtc ttgtcatact caatcaaatc
     1621 acccaagtaa aagaagcctg tatagtcact tgcacgagca gcctgctgca tattatgcgt
     1681 tacaatgatg atggtaaagt ttttcttgag ctcaaacatg gtctcttcta gttgcatggt
     1741 cgcaatcgga tccaaggctg aggctggctc atccattaag aggatatctg gcttaacaga
     1801 gatggcacga gcgatacaga gacgttgttg ctgaccacct gataaggtca aggctgactt
     1861 gtggagatcg tctttaacct gatcccagag ggcagcctga cgaagggagg tttctacgat
     1921 ttcatctagg acttgcttat ccttaactcc agcacgttca tgcgcaaagg taatattacg
     1981 gtaaattgac ttagcaaatg gattggggcg ttgaaaaacc attccaatgt gtttacgcat
     2041 ttcataaacg ttgatttctg gacggttgac atcaattcca cgatagagaa tctgcccagt
     2101 tactttagca atatcaatag tatcattcat gcgattgaga ctgcgtaagt aggtagattt
     2161 ccccgatccc gacgggccaa tcaaagctgt aattttattt ctttcaaatt gcatatcaat
     2221 ccccttaatg gattcatttt taccatagta aacatggaca tccttagtag aaagggctac
     2281 tttttcttca ggaaaggtaa ggatatgctt ctcatcccag ttatatgttg acatggcttc
     2341 tcctttaggc agcggttaat ttcttgtgta gatagcttcc gaacttacga gctccaaagt
     2401 taaaaatcag gataaagatc aggagcacag cggcagaacc tgctgataca atggttccat
     2461 ctggaatagt gccttcacta ttgactttcc agatatggac agccaaggtt tctgcttgac
     2521 ggaagataga gatggggcta gtcacactga ggatattcca gttagaccag tcaagagctg
     2581 gcgccgattg ccctgctgta tagatcagag ctgcagcttc gccaaagata cgaccagatg
     2641 ccaagacgac acccgttaca atacctggaa gcgcttctgg aataacaaca tgaaccactg
     2701 tctcccagcg agaaatccca agagccagac cagcctcacg ttgggtatgg tgaacgtgtt
     2761 tcaaactatc ttctacatta cgcgtcatct gaggcaagtt aaagactgtc aaggccaagg
     2821 cacctgaaat gattgaaaat ccatactcaa actggactac aaagatcaag taaccaaaga
     2881 gacccaccac cactgatggt aaagaggaca agatttcaat acaagtccgc acaaagttgg
     2941 taacaggacc ttttttagca tattcagcca agtaaatccc agctcccata gaaagaggta
     3001 cagaaataat caaggtaatg accaatagga aaaaggaatt gtaaagctga atgccaatcc
     3061 caccacctgc ttgaaaagca gaagaccttc cagtcaagaa agaccaagag atatggggca
     3121 agccccgaac caagatatag agaatcaagg aagccaagat tgtcacaatg atgctagcaa
     3181 tcgtatagag gacagctgtt gcaagtttat ctaatttctt agcgcgcata atttttcttt
     3241 cctctttctt tcgtaatcaa tttaatcaca ctgttaaaaa ctaaactcat caagagcagt
     3301 accaaggcca gtgaccagag aacattatta tttacagttc ccatgacagt gttcccaatt
     3361 cccatagtta atatagaagt taaagttgca gctggtgtgg tcaaggaagt tgggataaca
     3421 gctgagtttc cgacaaccat ctggatagct aaagcctcac caaaggcacg cgccatccca
     3481 aagaccactg cagtgaaaat accagaacgg gccgccttca agatcacacg ccagatagtc
     3541 tgccagcgag tggctcccat agcgaaactg gcttcacgat aataacgagg aaccgcacgc
     3601 aagctatccg ttgtcataaa ggttacggtc ggcaaaatca tgacaaagag gacggaaatc
     3661 cctgacaaaa tcccaaaacc agtcccacca aagacactgc gaacaaaggg aacgacgact
     3721 tgcaagccaa taaatccgta cactactgaa ggaatcccaa ccaggagttc aatagctggt
     3781 tgcaaaatct tcgccccttt tggtgatact tcggtcataa aaactgctgc accaatagca
     3841 aagggggttg cgataagggc tgagagaatg gtaacgataa aggaacccaa aatcatagga
     3901 agggcaccaa attctttact agaaggattc caagttcctc ccaaaagaaa gtcaaagata
     3961 ttcacaccat tgacaaagaa ggtcgacaag cctttttgcg ctacgaaaac caaaatcatg
     4021 gccacaagga tgactatcaa agaaagacag gcaaaggtca aaccttttcc taatttctcc
     4081 agacgagaat tctttgatgg aagcaacatt ttcttagcta attcttcttg attcattatt
     4141 gtctcccttc caacactgtc acagttccgg cagcatcttt ttcaaccttc atttccttaa
     4201 tcggaatata cttcaatcct ttgacaatcc cttcttgggt ctcatccgag agaacaaaat
     4261 tgagaaattc tgcagccaac tcattgggct gccccaatgt atacatatgc tcataagacc
     4321 acaagggcca attattgcta cttatatttt ctggacttaa gtcatagcca ttcaacttca
     4381 tgcttttgac cgaatcatct atataggtaa gagataaata agagatagct cctggacttt
     4441 ttgatacgat tgattttacc gctccatttg aatcctgctc ctgactttgc atggcagact
     4501 gaccttccat aatgacagta tcaaaggtag cacgagaacc agagccggct gcccgattga
     4561 taacagagat gggtaagtcc ttaccaccaa cctctttcca attggttacc tcacctatga
     4621 agatttgacg aagttgctct gtcgttaggt tatcaacatc aacctcctta ttgacaatca
     4681 gagccaagcc agctaccgcg accttgtggt caacaagagc agaagcatca attccgtctt
     4741 tttcctcagc aaatacatct gagtttccta tatcaactgc cccagactga acctgggaca
     4801 agcctgtacc agaacctccc ccttggacat tgaccgtttt tccaacatgg atcgtgccaa
     4861 attcatctgc cgctacttca accaagggtt gcaaggcagt tgagccaaca gccgttatgg
     4921 attctccacg atcaatccag ctagcacagc ctactaaaca agccgtcagc caaaaagcga
     4981 taagagacag agcaagcttt tttctttttt tcactgtttt tctcctcgaa aataattatg
     5041 aatactgtga attttttaag tagttcttta tgagttgacg catgaattct taccaaattt
     5101 ctgcgcaatt gattatttat ataatatagg ctatattact ctttcctaac ctcctttttt
     5161 catatgtgga taaaatctct tgtctatccc ttcccccatt gtcacccatt atagtcattt
     5221 cgtgtctctt tttccccttt ttaatgcaag ggaaattact ctccttagat gataatccaa
     5281 aagctagaaa ggtatctcaa acctctctac tctcccagac tagtttacaa ctaaaaggaa
     5341 aagattctat tttatgagaa atctagttta caagcggtaa gaacgctaat aactaaactt
     5401 cttgtactct ttgaaaatct cttcaaacca gtgttttgag ctatctatgg ctagcttcct
     5461 agtttgctct ttgattttca tcgagtagta aaactacatg tgatggcaat caagatatca
     5521 agaatcatcc tactaaaaaa atccatactt tcactataac atagaataag atatttgact
     5581 agcattttca tttgaatctg aggccttttg gaaaataatt tttcaaaaca tttccagtaa
     5641 cctttgcaaa gcccaagcca ttgcctttaa ccaaaacttg gtaccaacca tttggcagac
     5701 tttctgccag ctgaacggtt tctccagccg catacttgac aaacgcttct tggccaattt
     5761 caaccgactg ttcgacctga ctcggtttca aggctaaacc aagagcgaaa ctgggctcaa
     5821 agcgtttctt cttaaaagta cccagatgca gtccattgcg agcaatcttg agcttcccta
     5881 aatctggcaa aagttctggc aagagataaa gctggtctcc aaaaatctgc aagatacccg
     5941 gtagattgac cttcaaatgg ttttgggcaa attcctgcca caaggcaact tgttcacggc
     6001 tgaggttact cttacttgcc ttaaatttag gagctggatt gttaccctta aactgtagat
     6061 gggcaacaaa ctgaccctct cccttaaact gatgagggta catccgagcc gtttctggca
     6121 ggtcaatacc agctaccatt ccattgatat gctctactgg caacaagtca aaatcatact
     6181 cttccagcaa ccaattgaca atctcttcgt tttcctcggg tgcccaggta caggtcgaat
     6241 aaaccagacg accaccttca gctaacatgg tcactgcatc ctccagaatt tctctttgca
     6301 agctagcaca ttgactcgga taatctaagc tccaatagtc catagcatca ggttgcttac
     6361 gaaacattcc ttcaccagag caaggggcat caagaacgat taagtcaaaa tagcctttaa
     6421 agaccttgac caagcggtcg gcagattcat tggtcaccac gacatttgtc gctccaaaac
     6481 gctccatgtt ttcaaccaaa atcttagccc gtttgcttga aatttcattg gaaacaagta
     6541 gcccctcccc tgctagatag gctgccagtt gagttgattt gccccccggt gcagcagcca
     6601 agtccaagac cttcatacca ggactgggtt gggctacttg agccaccatt tgagcagcag
     6661 gttcttgcga ataaactaaa cctgtagcat gctcaggcga tttccctgaa accttcccat
     6721 agtggcccca aggggtttga gtaatggcat cagaaaagga aagttgctct tcttttaagg
     6781 gattgacccg aaaggccgaa accgcttcct cctcaaaaga ggcaagaaaa tctcttgcct
     6841 catctcctag tatctcttta tatttttcaa caaatccttc tggaaattgc atttaagttc
     6901 ttttcctttc gtaaatatag gactgaattt cctcctgcat ctcaagaggc accatcatga
     6961 ccggctgtct ggtttgaaaa tcaggagctt caccaaaaag ggtcacaacc cgatagccca
     7021 gactttcccc taaaatacta gctgcggcat aatcccatgg ttgcagataa gtgagatagg
     7081 tcaacaaacg ccctgacaaa atcttggcaa aactaatggc cgcacttcca tagacacgaa
     7141 caccaagaac cgctcggctc aaatcagcca gcccccattc attggtttcc agcataccac
     7201 tattccctgc aatgagaaaa tctccaagtg gtttagtttt aaaaggagct aggggcctat
     7261 catttagaca aactggaaat tccccaccac cgtggtaaca atcccctttg accacatcat
     7321 aaatcagacc aaactgtccc tgaccatttt caaaataagc catcataaca gcaaaatctt
     7381 cctgctgggc tacaaaatta ttggtaccat caatgggatc aatgacccaa accttgccat
     7441 cttgaaccga ggctcgcaga caaccttctt cagcacaaat cttatcctca ggataacggg
     7501 acaaaatctc accaaccaag agttcctgaa cttctttgtc cagtctggtc accaaatctg
     7561 ttggagaaga cttggtttca acacgcaagt cttcctgcat atggtcaaga atgtactgac
     7621 ctgctttctt aacaagctct ttagcaaatt caaatttact ttccaagaga aatctttcct
     7681 tccccttttt ctttggcagc ctgaactgct cggtaaagtg aatagccact aaccgtttca
     7741 aattctcgtc ccaaacgttt ctcttcgctc ttgcttggca cgaccgcttt gaatccctta
     7801 taggagtcca gtaacttttt agcctctact ttgccttcat aggcagcttc aacatcatta
     7861 aaaaaagaaa gcactgaagc aagttcttca gtgctccacg acaaatctag tgggtaacta
     7921 tactgtttgt tcattaacta ataccagctc tcattcttgc ttcttttagt tcttgcttac
     7981 gataactacg agggagaaaa gcacgaatct catcttcatt aaaaccgatt tgcatacgct
     8041 tggcatcaat aataattgga cgacgcaaaa gactaggata ctgctcaatc aaatgaagca
     8101 attccgatac cgaaatactc tctacatcaa tattcaattt ttgaaaaatt tttgaacgag
     8161 ttgaaatgat gtcatcagta ccattttcgg tcaaggaaag gatgtgttgc aattcttttc
     8221 ttgttaaagg actggtcata atattgtgtt ccacaaaggg aaccttatgt ttttctaacc
     8281 aggccttagc cttacgacat gatgtacagc tcggtgatag aaatagtgta atcatgcttt
     8341 tctcttctta tctatacttt gctacttcta ttatacaaaa aaataaagcg cttgactagg
     8401 gatttttaga aaaaaagcct attttttcaa gaaaaatagg ctgtttgcga acgattgaca
     8461 caattggatt tggttaattc actcttaacg a
//
LOCUS       DAVGJL010000092         8413 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.093, whole genome shotgun sequence.
ACCESSION   DAVGJL010000092 DAVGJL010000000
VERSION     DAVGJL010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8413
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.093"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(<1..1165)
                     /locus_tag="VVE42_001598"
     CDS             complement(<1..1165)
                     /locus_tag="VVE42_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HEU3073771.1"
                     /translation="MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSL
                     GVLTSLPLLMFTLFSPFSTRLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLM
                     VGASVAVINVLLPSLIQTNQPKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGL
                     ILLLTLLCLATFLVWLPNHRYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFY
                     TVMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVS
                     LAGVVGISMLFFPINNFIYWLAIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGL
                     SQTGGYILAAFGPTLFGYSFDLFHSWVPSVAALLLIDILMTVALFTVDRADKIL"
     gene            1223..2380
                     /locus_tag="VVE42_001599"
     CDS             1223..2380
                     /locus_tag="VVE42_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU3073772.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIEWWKEKFRRVVVTQNVKSLLVSIV
                     ISAYNEEKYLPGLIEDLKNQTYPKENIEILFINAMSTDGTTAIIQQFIKEDTEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGDLILKIDAHSKVTESFVMNNVAIIQQGEFVCGGPRP
                     TIVEGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREHGYKIRYSPSILSYQYIRPTFKKMLHQKYSNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPITFVFITLLLGAYFLLLSLLTLLTLLKHKNGF
                     LIVMPFLLFSIHFAYGLGTIVGLIRGFKWKKEYKRTIIYLDKISQINKNML"
     gene            2422..4272
                     /locus_tag="VVE42_001600"
     CDS             2422..4272
                     /locus_tag="VVE42_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEU3073773.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMIGFWGLNASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVARAVDEAKVPKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE"
     gene            complement(4628..4705)
                     /locus_tag="VVE42_001601"
                     /pseudo
     CDS             complement(4628..4705)
                     /locus_tag="VVE42_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(4755..5387)
                     /locus_tag="VVE42_001602"
     CDS             complement(4755..5387)
                     /locus_tag="VVE42_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU3073774.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAVQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPL
                     YITTTKDTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLAPEQAIII
                     GDTKFDMLGARETGIQKLAITWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
     gene            complement(5409..6281)
                     /gene="sdaAA"
                     /locus_tag="VVE42_001603"
     CDS             complement(5409..6281)
                     /gene="sdaAA"
                     /locus_tag="VVE42_001603"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEU3073775.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIENGKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            complement(6290..6961)
                     /gene="sdaAB"
                     /locus_tag="VVE42_001604"
     CDS             complement(6290..6961)
                     /gene="sdaAB"
                     /locus_tag="VVE42_001604"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEU3073776.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
     gene            7203..7790
                     /locus_tag="VVE42_001605"
     CDS             7203..7790
                     /locus_tag="VVE42_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU3073777.1"
                     /translation="MKSITKKIKATLAGVAALFAVFAPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVGQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVAETPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNG
                     DYSAENQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     gene            complement(7843..8163)
                     /locus_tag="VVE42_001606"
     CDS             complement(7843..8163)
                     /locus_tag="VVE42_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEU3073778.1"
                     /translation="MQRLEVYKNYQHLYDLRIAILLNLSTLYLYNQDKNMCKQICYTL
                     LEDAKNKKSYDRLAICYVPIGICRDDAKLIQKGFSLLELTEETSMLSHLKKEVETHYQ
                     PKKL"
BASE COUNT         2509 a         1560 c         1852 g         2492 t
ORIGIN      
        1 aaaggatctt atccgctctg tccactgtaa agagggccac agtcatcagg atatcgatga
       61 gcaagagggc agctacagat ggtacccaag agtggaacag gtcaaaactg taaccaaaga
      121 gggttggccc aaaggctgct aggatatagc ctcctgtttg agataggccg gacaattggg
      181 ctgtcttttc aggggcgctt gtcttgagtg aaaagttgac catgagataa gggaagaggg
      241 cgctggttgc ggttccgatg aggagatgga tggcaagcca gtaaatgaaa ttattgattg
      301 ggaaaaagag catggaaatg ccgaccacac cagctagtga aaccagagtg agcatgagct
      361 gacggttgcg agtagataaa ctggttgtca ggcttgggat ggtcattgaa aaaggaatgc
      421 taatcagaga taagatagaa gtcagcaagc cagcttcgtg actggataga cctgcatgga
      481 tagacatggt aggtaaccag gtcatgacgg tgtaaaagat caaggattga aaacctgaaa
      541 agataataat tgcccaaacc tgtttattac gcatgacctt tatttgactt ttttgtttgg
      601 tttgtggagc tagtctatga ttatagcggt gatttgggag ccagaccaaa aaagttgcta
      661 gacagagtaa cgtgaggagg aggataagtc ctttccaaga actggcttgt gtaatgggca
      721 cagctagata ggaagccaga gccgttgcaa tccccataga cgttacatat aaggtggtca
      781 gaaaaccaat tttctttggt tgattggttt ggataagact aggaagcagg acattgatga
      841 ctgcgacact tgccccaacc atcaaggttc ctagatagag caggggcaga ttgattagtc
      901 gaatgagtga gccgatggtc aagaagaaga ggctgtaggt gaagagatgc tccaagccga
      961 ttttctgagc cagtcgggta gaaaatggtg aaaagagggt aaacataagg agaggtaggc
     1021 tggtcaagac accaagcgaa ctaacttcta cctccagacc ttgcgaaata tttcccaaaa
     1081 taatgggtaa aacagtaaaa ggagttcgca aggaaacacc aatcaggata atacctggaa
     1141 caaaaaagag tgattgcttt ttcatataat acctcttcta gctgatttta ataacagctt
     1201 attttaaggc tttttcacta taatgtcaag taaggtttct ggctttcaag ataaaaatgg
     1261 gaaagtcttg aaaattatga tagaatggtg gaaggaaaaa ttcaggagag tagtagtgac
     1321 tcaaaatgtt aaaagtcttc tcgtatccat tgtaatcagt gcatacaatg aagaaaaata
     1381 tctgcctggt ctaattgaag acttaaaaaa tcaaacttat cctaaagaga atattgaaat
     1441 tctatttata aatgctatgt ccacagatgg gaccacagct atcattcagc aatttataaa
     1501 ggaagataca gagtttaact caattagatt gtataacaat cctaagaaaa atcaagctag
     1561 tggttttaac ctgggagtta aacattctgt aggggacctt attttaaaaa ttgatgctca
     1621 ttcaaaagtt actgagagtt ttgtaatgaa caatgtggct attattcaac aaggtgaatt
     1681 tgtctgtggg gggcctagac cgacgattgt cgaaggaaaa ggaaaatggg cagagacctt
     1741 gcatcttgtt gaggaaaata tgtttggcag tagcattgcc aattatcgaa atagttccga
     1801 ggatagatat gtttcttcta tttttcatgg gatgtataaa cgagaggttt tccagaaggt
     1861 tggtttagta aatgagcaac ttggccgaac tgaagataat gatattcatt atagaattcg
     1921 agaacatggt tataaaatcc gctatagccc aagtattcta tcttatcagt atattcgacc
     1981 aacattcaag aaaatgctgc atcaaaagta ttcaaatggt ttgtggattg gcttgacaag
     2041 tcatgttcag tttaagtgtt tatcattatt tcactatgtt ccttgtttat ttgttttgag
     2101 tcttgtgttt agtctagcat tgttaccgat cacattcgta ttcataactt tactattagg
     2161 tgcctatttt ctacttttgt cattactcac tttgctgact ttattaaaac ataaaaatgg
     2221 atttctaatt gtgatgccct ttcttttatt ttccattcac tttgcttatg gccttgggac
     2281 gattgtaggt ttaattagag gatttaaatg gaagaaggag tacaagagaa caataattta
     2341 tttggataaa ataagccaaa taaataaaaa tatgctataa taacaatata gtaaaactct
     2401 tttaaggagg agtagatttc tatgaataaa aaactaacag attatgtgat tgatctggtg
     2461 gaaattttaa ataaacaaca aaagcaggtt ttctggggaa tatttgatat tttcagtatg
     2521 gtggtttcca taattgtatc ttatatttta ttttatgggc tgattaatcc agcacctgtt
     2581 gactacatta tctatacgag tttggccttc ttgttctatc aattgatgat tggtttttgg
     2641 gggttgaacg cgagcattag tcgttacagc aagattacgg atttcatgaa aatctttttt
     2701 ggtgtgactg ctagcagtgt cttgtcatat agtatctgtt atgccttctt gccactcttc
     2761 tccatccgtt tcatcattct ctttatcttg ttgagtacct tcttgatttt attgccacgg
     2821 attacttggc agttaatcta ctccagacgc aaaaaaggta gtggtgatgg agaacaccgt
     2881 cggaccttct tgattggtgc cggtgatggt ggggctctct ttatggatag ttaccaacat
     2941 ccaaccagtg aattagaact ggtcggtatt ttggataagg attctaagaa aaagggtcaa
     3001 aaacttggtg gtattcctgt tttgggctct tatgacaatc tgcctgaatt agccaaacgc
     3061 catcaaatcg agcgtgtcat cgttgcgatt ccgtcgctgg atccgtcaga atatgagcgt
     3121 atcttgcaga tgtgtaataa gctgggtgtc aaatgttaca agatgcctaa ggttgaaact
     3181 gttgttcagg gccttcacca agcaggtact ggcttccaaa aaattgatat tacggacctt
     3241 ttgggtcgtc aggaaatccg tcttgacgaa tcgcgtctgg gtgccgaact gacaggtaag
     3301 accatcttag tcacaggagc tggaggttca atcggttctg aaatctgtcg tcaagttagt
     3361 cgcttcaatc ctgaacgcat tgtcttgctc ggtcatgggg aaaactcaat ctaccttgtt
     3421 tatcatgaat tgattcgtaa gttccaaggg attgattatg tacctgtgat tgcggacatt
     3481 caagactatg atcgtttgtt gcaagtcttt gagcagtaca aacctgctat tgtttatcat
     3541 gcggcagccc acaagcatgt tcctatgatg gagcgcaatc caaaagaagc cttcaaaaac
     3601 aatatccgtg gaacttacaa tgttgctaga gccgttgatg aagccaaggt gcctaagatg
     3661 gttatgattt cgacagataa ggcagttaat ccaccaaatg ttatgggagc aaccaagcgc
     3721 gtggcggagt tgattgtcac tggctttaac caacgtagcc aatcaaccta ctgtgcagtt
     3781 cgttttggga atgttcttgg tagccgtggt agtgtcattc cagtctttga acgtcagatt
     3841 gctgaaggtg ggcctgtaac ggtgacagac ttccgcatga cccgttactt tatgaccatt
     3901 ccagaagcta gccgtctggt tatccatgct ggtgcttatg ccaaagatgg ggaagtcttt
     3961 atccttgata tgggcaaacc agtcaagatt tatgacttgg ccaagaagat ggtgcttcta
     4021 agtggccaca ctgaaagtga aattccaatc gttgaagttg gaatccgccc aggtgaaaaa
     4081 ctctacgaag aactcttggt atcaaccgaa ctcgttgaca atcaagttat ggataagatt
     4141 ttcgttggta aggttaatgt catgccttta gaatccatca atcaaaagat tggagagttc
     4201 cgcactctca gtggagatga gttgaagcaa gctattatcg cctttgctaa tcaaacaacc
     4261 cacattgaat aaaaaagaaa aacgcatagt atcaagtttg ttaaccttgg tactatgcgt
     4321 tttattatgt agagacttat actcttcgaa aatctcttca aaccacgtca acgtcgcctt
     4381 gccgtatatg gttactgact tcgtcagttc tatccacaac ctcaaaacag tgtttagagc
     4441 tgacttcgtc agttctatcc acaacctcaa aacagtgttt agagctgact tcgtcagttc
     4501 catctacaac cttaaaacag tgtttagagc tgacttcgtc agttccatct acaaccttaa
     4561 aacagtgttt agagctgact tcgtcagttc catctacaac ctcaaaacag tgtttagagc
     4621 tgacttcgtc agttctatct gcaacctcaa aacagtgttt agagcagccc gcagctagtt
     4681 tcctagtttg ctctttgatt ttcattgagt attacttcat tttcttctga aatggaattg
     4741 ttacccagtc tatgctattg aaaatacgcc aaaacttcta agggtttgtg agcgatataa
     4801 tcaggttgat agtttagtag atctgcttgc tctccaaatc cccaagtgat ggccaatttc
     4861 tgaatacctg tttctcgagc tcccagcata tcaaacttgg tatctccgat gatgatggct
     4921 tgttctggtg ctagttgatg tgtctgcaag gcttggtgaa tgacatctgc cttatggggt
     4981 gcttcagggc tagaaccata aatgccatca aagaaatgat ggatttccaa gttttttgcc
     5041 atgtcttgag cagtagatgt atcctttgtc gtggtgatgt agagtggata actgctcgat
     5101 aactcctcaa gcaagtctat aatctgagga aagagttgag cttcatagat gccttttgcc
     5161 ttatagtaag aacgatatat ctgcacggct tcagaaattt ggtctttgga caggcaggtc
     5221 gcaaaactac tttcgagagg tggtcccata aaaccacgaa tagttttggc atcagggcta
     5281 ggcaccccca gctctttaaa ggtataggta aaggcattgt gaatcccgat agaactatca
     5341 acgagggttc catccaaatc gaaaaaaatc gctgtgatag aggtcatggt ttctcctatt
     5401 tgataagctt attctccgaa aatttctttt tggaggcgac gaccagtagg ggtggtagcg
     5461 agtccacctt cagctgtttc acgaaaggca gttggcatgc ttgctcctac ttggtacatg
     5521 gcatcgatca cttcatccac agggatttta gattcgatac ctgccaaggc catgtctgct
     5581 gcgatgaaag caaagctagc tcccatggca ttacgtttga cacagggaac ttcgaccaaa
     5641 cctgcaacag ggtcacagat gaggcctagc atatttttaa tgacaaaggc aatagcttga
     5701 ctggcctgat aaggtgttcc acctgcagcc agagtcaagg cggcagcact catagcagag
     5761 gctgaaccaa cttcagcttg acacccaccc tcagcacctg agatggaggc attgtttgcg
     5821 atgactagtc caaaggcacc agcagcaaag aggaaatcca attgttgctc gtggctgagg
     5881 tctaattttt caatagcagc agtgagaacg gatggcagac agccagcact tccagcggtt
     5941 ggagtggcac agaccaagcc cattttggca ttgtgttcat taactgcgat ggcatttcgg
     6001 gcagccgaga gaatcgtata atctgacaga gtttttccgt tttcgatgta gtgatccaat
     6061 ttggcagcat ctccacctgt caggccacta cgagatttat tttcattgag gccaagttgg
     6121 acagaggctt tcataacttc cagattgcgt tccatgagaa ggaagacttc ttcacgttcg
     6181 cgaccggtca attcaaactc tgttgtaatc atgagttctg cgacatttcc ttgaaagtcc
     6241 agatctgctt gctcgaccaa ttctttgata gaataaaaca tgcttcctcc tatttaaaga
     6301 aattgacatt gtggagatga gggatttttc gaatttcttc gatagcctca tcacagttgc
     6361 gactgtcaac ttcgataatc ataatggctt tttcaccagc tttttcacga gtgacattca
     6421 tctgggcgat attgatacca tagcgggaaa gcgcctctgt aacaagggca atcatacctg
     6481 gaatatcttg atgaacgatg atgatagtcg gtgtattcat attgagagag acggcaaaac
     6541 cattgagttc ggttacctga atatttcctc caccgataga aataccagtc acgctgatgg
     6601 tcttgtgggc atttttaaca gtaattttag tggtgttagg gtgaggggca ttgctgtctt
     6661 tctgaatggt ccagacaatc ttgataccac gcttgtgggc aatttccaga ctatttggaa
     6721 tttcaggatc atctgtatcc attcctaaaa tacctgcaac aagggctagg tctgttccgt
     6781 gaccacgata ggtcttggca aatgagttaa aaagttggaa ttcaacttct gtcggagtat
     6841 catcaaaaat ggaagagaca atcttcccaa tacgaacagc accagcggta tggctactag
     6901 atgggccaat cataactggt ccgatgatat caaagacaga ttgaaaacga agtgatttca
     6961 tcagtttccc cttataaaaa ttcttatctc tattatatca aagaatgagg ggcttggctt
     7021 taattgtgga tgaaaacctt tctaatacct caaatagcat aaaaatagta tcttttatga
     7081 caaaaaacac cttatttagg gaaataaaaa ataattttgt aatatttcta cataaaagtg
     7141 tcaagaaacg gtaatattta aagggtatga tagaactata gaaagaagga gaattttcga
     7201 atatgaaatc aataactaaa aagattaaag caactcttgc aggagtagct gccttgtttg
     7261 cagtatttgc tccatcattt gtatctgctc aagaatcatc aacttacact gttaaagaag
     7321 gtgatacact ttcagaaatc gctgaaactc acaacacaac agttgaaaaa ttggcagaaa
     7381 acaaccacat tgataacatt catttgattt atgttggtca agagttggtt atcgatggcc
     7441 ctgtagcgcc tgttgcaaca ccagcgccag ctacttatgc ggcaccagcc gctcaagatg
     7501 aaactgtttc agctccagtg gcagaaactc cagtagtaag tgaaacagtt gtttcaactg
     7561 taagcggatc tgaagcagaa gccaaagaat ggatcgctca aaaagaatca ggtggtagct
     7621 atacagctac aaatggacgt tatatcggac gttaccaatt aacagattca tacctgaacg
     7681 gtgactactc agctgaaaac caagaacgtg tagcagatgc ctatgttgca ggacgttacg
     7741 gttcatggac agctgctaaa aacttctggc ttaacaatgg ctggtattaa gaactaacga
     7801 acaaataata tgtaaagagt cgaggaaatc cctcgacttt ttttataatt tctttggttg
     7861 ataatgggtc tctacttctt ttttgagatg agacagcata gaagtttcct cggttagctc
     7921 taggagtgag aaaccttttt ggataagttt cgcatcatcc ctacaaatcc caatagggac
     7981 atagcagata gccagcctat cgtagctttt cttattcttg gcgtcctcca gtaaagtgta
     8041 gcagatttgc ttacacatgt ttttatcttg attgtataga tagagtgtgg aaaggttgag
     8101 caggattgct attcgcaagt catataaatg ttgatagttt ttatagactt ctaagcgttg
     8161 caagattttc tcggattatt gacaaataaa cggacattct gatagacgat tgccctagcg
     8221 gcttagtgta aactttacaa atttggcttg taattttttt ataaaattca cttcagttac
     8281 ttgacagtta ttatattttc acttattata tagataaaag gatgggatag gagtttaaaa
     8341 atttgaaagc gctatccata atcataacgc ataattttct ccttttcgtg aaaaaagtta
     8401 acgtaatttt gaa
//
LOCUS       DAVGJL010000093         8269 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.094, whole genome shotgun sequence.
ACCESSION   DAVGJL010000093 DAVGJL010000000
VERSION     DAVGJL010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8269
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.094"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(64..267)
                     /locus_tag="VVE42_001607"
     CDS             complement(64..267)
                     /locus_tag="VVE42_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU3073779.1"
                     /translation="MIKLYSPYAFLSKYLGAILLSFFCYYAKEIGCNNLTLSVWNDNE
                     GALRFYQRQGMKPQETTMEMIID"
     gene            complement(260..1342)
                     /locus_tag="VVE42_001608"
     CDS             complement(260..1342)
                     /locus_tag="VVE42_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEU3073780.1"
                     /translation="MSKLQQILTYLESEKLDVAVVSDPVTINYLTGFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYHGLKTVFETAEFDNLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDRAARE
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFDLFTSTSKDLLYFD"
     gene            complement(1468..2250)
                     /locus_tag="VVE42_001609"
     CDS             complement(1468..2250)
                     /locus_tag="VVE42_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEU3073781.1"
                     /translation="MVYTSLSSKDGNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGAHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            complement(2250..3593)
                     /locus_tag="VVE42_001610"
     CDS             complement(2250..3593)
                     /locus_tag="VVE42_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEU3073782.1"
                     /translation="MNLKTTLGLLAGRSSHFVLSRLGRGSTLPGKVALQFDKDILQNL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRYGEIYTTYKMILDAIRKVPT
                     ATVLLNGDSPLFYKPAIPNPVEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGAYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDGQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQLIKQGFDKSRAVFGRQETFHIGDKECTLVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
     gene            complement(3692..5008)
                     /locus_tag="VVE42_001611"
     CDS             complement(3692..5008)
                     /locus_tag="VVE42_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEU3073783.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASADKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYTTVAGTGVDIFDAEAHFLS
                     NKVIEIQAGDEKQELTAETIVINTGAVSNVLPIPGLATSKNVFDSTGIQSLDKLPEKL
                     GVLGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYLEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGAFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
     gene            5201..6427
                     /locus_tag="VVE42_001612"
     CDS             5201..6427
                     /locus_tag="VVE42_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEU3073784.1"
                     /translation="MKSNRYIIAFAGVILHLMLGSTYAWSVYRNPIIEKTGWDQASVA
                     FAFSLAIFCLGLSAAFMGRLVEKFGPKVMGSLSAFLYAGGNILAGFAIDRQELWLLYL
                     AYGILGGLGLGAGYITPVSTIIKWFPDKRGLATGLAIMGFGFASLLTSPIAQHLIAGV
                     GIVETFYILGASYFIIMLLASQFIKRPNEQELAILSSSGKEKTASLTQGMAANQALKS
                     NRFYMLWIIFFINIACGLGLISAASPMAQEMAGLSTSHAAVMVGVLGIFNGFGRLLWA
                     SLSDYIGRPLTFSILLLVNLFFSLSLWLFTDSVLFVVAMSILMTCYGAGFSLIPAYLS
                     DIFGTKELAALHGYILTAWAMAGLAGPILLAETYKMAHSYTQTLFVFLILYSIALALS
                     YYLGRSIKKESQKPLT"
     gene            6626..8200
                     /locus_tag="VVE42_001613"
     CDS             6626..8200
                     /locus_tag="VVE42_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU3073785.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRTEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQDIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVAPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRGNGRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNT
                     LSEKQTGFVIRNKGDK"
BASE COUNT         2360 a         1617 c         1842 g         2450 t
ORIGIN      
        1 gaagattttc ctcattttct agtgaaattg aactttttct aacatctttt gatagatgac
       61 ttcttaatca attatcattt ccattgttgt ttcttggggt ttcatccctt gtctttgata
      121 aaagcggaga gcaccctcgt tatcattcca gacacttagg gttagattat tgcagccaat
      181 ttctttagca taatagcaga aaaatgacaa taaaatagcc cctagatatt ttgaaaggaa
      241 agcatagggg ctatatagtt taatcaaaat aaagcaaatc tttgctggtg cttgtaaaga
      301 ggtcgaagcc atccttggta acaacaccgc agtcttcaat acgaacaccg actttaccag
      361 ggatatagat acctggttca acagagaagc acatgccttc ttcgatgacc atgtcgtttc
      421 cttccatgat agatgggaat tcatggacat ccataccgat accatgcccg agacggtggt
      481 tgaagtactc accataacca gctttttcga tgacctcacg ggcagcgcgg tccacttcat
      541 gagcagtcac acctggcttg ataaagtcaa gagcagcttg ttgggcttca agagtcaagt
      601 tgtaaatatc tttcttgaat tggtctggtt tgccgacagc gactgtacga gtcatatctg
      661 acgcatagcc attgaccaga acacccaggt caaagaggag aagagcatca ttttcaacct
      721 tattagctgc tggaatgccg tgtggattcg cagcattatc accagtcaag accatggtat
      781 caaagctcat ttcgtaacct tcacgtttca tggcaaagtc aatttgtgcg atgatatctg
      841 tctcagtctt atcaagagaa atattgtcaa aaccaacatg aacagccttg tcagcataaa
      901 gacctgcaac catcattttt tgcacttcat cagctgattt gatgaggcgc atgcgttgga
      961 tacgaggagt gaggttgtca aactcagcag tctcaaaaac tgttttcaaa ccatggtatt
     1021 tggtcaagat gagattgtca aactcaacag cgacacgttt gaagtcaagt tgtggaagag
     1081 catgtttgat tttttgccat ggattttcag aatcgacata gcccactact gggaaggaaa
     1141 cggtgctact tgcacgttct acttcaagag ctgggacaaa gaggagaggt tcctgatccg
     1201 ctaggacaaa gaggaacatt tggcgttcat ggggatcact gtaaaaacca gtgaggtaat
     1261 tgattgtgac ggggtcagat acgacagcga cgtctagttt ttctgattca agatatgtta
     1321 ggatttgttg taatttagac atatgctacc ttctttctac ccctctattt tggcaaaaaa
     1381 tgagagaaaa tgcaagggag aagggtaaaa gaaaagacaa aaaactccga caagataacg
     1441 gagttctttg atatcatttt cctttgttta agaaaagtca gccttgcttt tgacgtcact
     1501 gtactcttca gcgatttctt ggctgaggat atcctcatag gcagggagtt ggatatcctg
     1561 accatatttc ttcttgaggg cagtagtaac taggcgataa gccagattgg cattacgaga
     1621 gaggatagga ccgtggaagt aggaaccaaa gacattctta taatgaaccc cttcaccgac
     1681 cttttcttcg ttgtttccat ttccatagac aacctgtccc agcggttttt ggtcatcaga
     1741 gaggaaggta cggccttggt gattttcaaa tccatagtag gtttcatcga aatcttcatt
     1801 gtgaatcttg atgtcaccga taaaacggtt attggtctgg ttgagcgtgt agtgtcccat
     1861 gacccctagc ccttcgatac gttttcctga agcttcaaca taatattgac ccaatagttg
     1921 gaaaccaccg cagatagcca gaactacacc gtcgttttgg atgtagttgt caatgctctc
     1981 ttttttagca ggtaggtcgt ctgcaatgat actttgttca aagtcttgac caccaccgaa
     2041 aaaggcgatg tcgtagtgat tttcatcaaa gtcatcatgg agagaaacga tgtcaacggt
     2101 cacatgggct cccagttttt cagccacata cttgagcatg aggatgtttc cattgtcccc
     2161 gtaggtattc atgagatttc cgtagaggtg ggcaatgttg agctgatagg ggtaattgcc
     2221 atcttttgag gaaagtgaag tataaaccat tagttcatct cctttctaac aatctgacga
     2281 ctagccagca gttcacgaaa ttccagcatg gcagtataag ttgccagaat ataggcatgc
     2341 ttgcagtctt gattctcaat ggtcttgaga acttgctcca gattactcgt ttcagtgatt
     2401 ttctcagctg gatagccagt cactcggagg cgacgagcga tttcagaatg acgaacaccg
     2461 ccagcgttga tttcaggaat gtccatgtca gtgatttgtt caaagtctgc atcccagatc
     2521 cagctagtgt caattccatc tgcatagttg gcattaagga ggacagatag gctaaatgga
     2581 taaggtgcta gtttgatcat ttcgatagct tgggttgcac cgactggatt tttaatcaag
     2641 acaagggtac attccttgtc accaatatga aaggtttctt ggcgtccaaa gacagcacgg
     2701 ctcttgtcaa atccctgttt gatgagttgg gaatctgcgc cgaggaaacg ggcgatggcc
     2761 acagcagcta gggcgttata gatattatag agcccgccga tttggatacc gtattcttgg
     2821 ccgtctatga caaagcgaga gcgattgttg gtcaactcaa ccagttttgt caaacgatag
     2881 tcgagatcag gacgtttaca tccacaacct tcacagatat aggcacccaa gtttgcatag
     2941 gtattatgct catatttgag gatgccttgg cagtcaggac agaggattcc ttcggtatta
     3001 tagtgagcca gctgggctgg acctttctcc aagtcaaaac caaaatactc tacaggattt
     3061 ggaatagctg gcttgtagaa aagtggactg tctccattaa ggagaacagt ggcagtaggc
     3121 accttacgaa tggcatccaa aatcatcttg taagttgtgt aaatctcacc atagcggtcc
     3181 atctggtcac ggaaaatatt agtaataaca aataagcttg gctggatata gtcacagata
     3241 cgagatagac tggcctcgtc gatttctagg acggcaatat ttttcccagt tttagaagat
     3301 ttggctgtta agaaggttgt cgcaattcct gtaatcatgt tggcaccgct tgggttggtc
     3361 agaacttgac cataaacttc ttttaaaata ccgacagtga gggcagttgt cagggttttt
     3421 ccatttgttc cagtgacaac gacaatctcg tagttcttag ctaggttttg taaaatatct
     3481 ttatcaaatt gaagggcgac tttacctggg agcgtacttc cacgtccaag acggcttaaa
     3541 acgaagtggg aagaacgccc agcaagaagg cccaaagtag tttttaagtt catgtttcta
     3601 ttatatcaca aaagagggaa aagacagatt tggtgtttcg tagaagtaaa aacagcccaa
     3661 agagggctgt cagttaagat gagatttcaa cttaaatcgc aaacaagtca ttcaagttct
     3721 cagccaaggt tgggtgagtg aaaatttgtt ttgtgaagta agtgtaagga atcttgttgt
     3781 ccatagcaac agtaatgatg ttgatgattt cttgagaacc ttctgagaag atgcttgctc
     3841 caagaatttc ttttgtttca gtattaacaa cagctttgaa agctccgcga aggtctccat
     3901 ttacgtgacc acgaggcatg gctgcaacag ggatttcttt cactgcgtat ggaagtttca
     3961 aatcagctgc ttggctttca gtcaaaccaa cttgtgaaag tgcaggtgtg atgaacatag
     4021 tatttggcac attgagacgg tcctcaagtg tgtagctgcc atctccagca aggtagctgt
     4081 aaacaacacg gaagtcatca agtgaaatgt aagtaaattg aagaccaccg ttgacatctc
     4141 caactgcaaa gacaccagga acgtttgttt gacagtgttt atctacttta atagcgccac
     4201 gttcagttag ttcaatatct gtattttcaa gttgaagtgg ttctacattt ggtttgcgtc
     4261 cagttgcgta gagaagggcg tcgaaacggt aagtttcgtc ttcagttacg acaagcactt
     4321 ggtcaccgtc gtttttaatt tcagtagtat ggatattttg aagcaattca ataccgtctt
     4381 cttccaggta ttgtttagca agagctgcga tggaaggttc tgcacgaggt aggaatgtat
     4441 ccaaggcatc taggactgta accttgcttc ctagtttatt gtaaaggcca gcaaattcaa
     4501 gaccgatatt tccgccacca aggactccaa gtttttcagg caatttatcc aagctttgga
     4561 tacctgttga gtcaaagacg tttttgcttg tagcaagtcc agggattggc aagacgtttg
     4621 aaacagcacc agtgttgatg acgatagttt cagcagtcaa ttcttgtttt tcatcaccag
     4681 cttggatttc gatgacttta tttgaaagga agtgagcttc agcatcaaag atatcgacgc
     4741 cagtaccagc aacagtcgta tagtttttac cgttgaggcg accagtgatc gtgtttttgg
     4801 tagcaatgac ttcttcaaaa gacaagtcct tttcagcagc aactagcaag gttttagttg
     4861 ggatacaacc gatgttgata caagttccac cgtacatagc tttgctacgt tcaacgaggg
     4921 caactttttt gtcagctgaa gccaatttac cagctagtgt tttaccagct ttaccaaatc
     4981 caataacaat taaatcgtat gttaacattt atcttcctcc tattcgaatt acattctagc
     5041 acaagaaaaa aacttttgca caattctgct acgaaaaaga cagaaaaaga taaaaacgct
     5101 ttccagacaa aaaactagta attttttaac aagcttgata aactaaaatc ctatctattt
     5161 ttatataaga tagaataaat gttcgaaaat aggaggtttt atgaaatcga atcgttatat
     5221 tattgccttt gctggggtca ttttacactt aatgctgggt tcgacttatg cctggagtgt
     5281 ttatcgtaac cctattattg aaaaaacggg atgggatcag gcttctgttg ccttcgcctt
     5341 tagtctagca atcttttgtt tgggcttatc ggctgcattt atggggcgtt tggtagaaaa
     5401 atttggtccg aaagtcatgg gaagtctatc tgcttttcta tacgcaggtg gaaatatctt
     5461 agcaggattt gcaatagacc gtcaggagct gtggttgttg tatctcgctt atggcatttt
     5521 aggtgggctt ggtttgggag caggctatat tacccctgtg tcgacgatta taaaatggtt
     5581 tcctgataaa cgtggtctcg caacaggttt agcgattatg gggtttggtt ttgcttcttt
     5641 attgactagt cccatagcgc aacacctcat cgcaggggta gggattgtag aaacttttta
     5701 tattttagga gcaagttact ttattatcat gctcctagct tcacaattca ttaagcgtcc
     5761 aaatgagcaa gagcttgcaa ttttatcttc ttcagggaaa gaaaaaacag cctctttgac
     5821 gcaaggaatg gctgcaaatc aggctctgaa aagcaatcgg ttttatatgc tttggattat
     5881 tttctttatc aacatagctt gtggtttagg cttaatttca gcggcatcgc caatggcaca
     5941 ggagatggct ggcttgtcta caagtcatgc agcagtaatg gtgggtgttt tggggatttt
     6001 caatggattt ggtcgcttgc tctgggcgag tttgtctgac tatatcggtc gccctctaac
     6061 ctttagtata ttactgcttg tcaatctttt cttttctctc tcactttggc tctttacaga
     6121 ttccgtttta tttgtagttg ctatgtctat tttgatgact tgctatggag ctggtttttc
     6181 tttgattcca gcttatctca gtgatatttt tggaaccaag gaattggccg ctctgcatgg
     6241 ttatatttta acagcttggg caatggctgg tttagcggga cctattttat tagcagagac
     6301 ttataaaatg gctcattcgt acacacaaac cttgttcgtt tttctcattt tatacagtat
     6361 cgccttggct ttgtcttatt atctaggtcg ttcaatcaaa aaagaaagtc aaaaaccgct
     6421 tacatgattt tctgtagaaa tggggctatc ttttgctctg gtcttgtgtt atactagata
     6481 ggttgcaaag aaaacagtac ttttcttttg tggaaaagaa gcaacacgat tttatatcta
     6541 gttatatgaa aatacgtcat aaaaagaaaa gtataaacta agccctatag ggtggcttcg
     6601 caccaccttt agaaagaaga ataacgtgaa atttaatgaa ttaaacttgt ctgctgattt
     6661 gctagcagaa attgaaaaag ctggttttgt agaagctagt cctatccaag aacaaactat
     6721 tcctttggcc cttgaaggca aggatgttat cggtcaagct cagactggta caggaaaaac
     6781 tgcagccttt ggcttgccta cccttgaaaa aatccgtaca gaagaagcga ctatccaagc
     6841 cttggtcatc gctccaactc gtgaactagc tgtccaaagt caagaagaac tcttccgctt
     6901 tggtcgtagt aagggagtca aagtccgttc agtatatggc ggatcaagca ttgaaaaaca
     6961 aattaaggct cttaaatctg gtgcccatat cgtggtggga actccaggtc gcctcttgga
     7021 cttgattaaa cgcaaggcct tgaaattaca agacattgaa acccttatcc ttgacgaagc
     7081 ggatgaaatg cttaacatgg gcttccttga agacatcgaa gccattattt cccgtgtacc
     7141 tgagaaccgt caaactttgc ttttctcagc aactatgcca gatgccatca aacgtatcgg
     7201 tgttcagttt atgaaagccc ctgaacatgt caagattgcg gctaaggaat tgacaacaga
     7261 attggttgac cagtactata tccgtgttaa ggaacaagaa aaatttgaca ccatgactcg
     7321 tctcatggat gtggcacaac cagaactcgc tattgtattt ggtcgtacca aacgccgtgt
     7381 ggatgaattg actcgtggtt tgaaaattcg tggcttccgt gcagaaggaa ttcatggcga
     7441 cctagaccaa aacaaacgtc ttcgtgtcct tcgtgacttt aaaaatggca atcttgatgt
     7501 tttggttgcg acagacgttg cagcgcgtgg tttggatatt tcaggtgtga cccatgtcta
     7561 caactacgat attccacaag atcctgagag ttatgttcac cgtatcggtc gtacaggtcg
     7621 tgctggtaag tcgggtcaat ctattacttt tgttgctcca aacgaaatgg gttaccttca
     7681 aatcattgaa aacttgacta agaaacgcat gaaaggtctc aaacctgcaa gtgtagaaga
     7741 atccttccaa tcaaaaaaac aggtagctct caagaaaatc gaacgtgatt ttgcagatga
     7801 aaccattcgt gccaactttg agaaatttgg taaggatgct cgcaaattgg ctgctgagtt
     7861 tactccagaa gaattggcaa tgtatatctt gagtctgaca gtccaagacc cagatagcct
     7921 tccagaagtg gagattgcac gtgaaaaacc actaccgttt aaaccatcag gtaatggttt
     7981 cggtggtaaa gctaagggag gtcgtggagg ccgtcgtggg gacgaccgtc gagagcgtga
     8041 tcgccgtggc aatggtcgcc gtgatgagtt caaaaaagga agtcgtggca acgatcgttt
     8101 tgataaggaa aaacgttacc gtaaggataa taaaaaacca cgcaatactt taagcgaaaa
     8161 gcaaacaggc tttgttattc gtaacaaagg cgataaataa gaaaaagcgg ttcaaaatga
     8221 atcgcttttt tatataagga gaccgaatgt gaaaagacaa ataaaatta
//
LOCUS       DAVGJL010000094         8234 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.095, whole genome shotgun sequence.
ACCESSION   DAVGJL010000094 DAVGJL010000000
VERSION     DAVGJL010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8234
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.095"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(1..290)
                     /locus_tag="VVE42_001614"
                     /pseudo
     CDS             complement(1..290)
                     /locus_tag="VVE42_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(336..569)
                     /locus_tag="VVE42_001615"
     CDS             complement(336..569)
                     /locus_tag="VVE42_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU3073786.1"
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQNVGDVVKIIQGK"
     gene            complement(575..1567)
                     /gene="plsX"
                     /locus_tag="VVE42_001616"
     CDS             complement(575..1567)
                     /gene="plsX"
                     /locus_tag="VVE42_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEU3073787.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIK
                     QYLTATERVSIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFIVGRIKNIDRPGLMSTLPTVDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLVADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIMGLLKTAITGGGLRAKLGALLLKDSLRGLKKQLN
                     YSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREFSGE"
     gene            complement(1564..2334)
                     /gene="recO"
                     /locus_tag="VVE42_001617"
     CDS             complement(1564..2334)
                     /gene="recO"
                     /locus_tag="VVE42_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEU3073788.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKRMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGACLCPEHYHEDKRRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            complement(2331..3500)
                     /locus_tag="VVE42_001618"
     CDS             complement(2331..3500)
                     /locus_tag="VVE42_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU3073789.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQVISEIPGVLRLTLGEPDFTT
                     PDHVKEAAKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKAVILNYPANPTGITYSREQLEALAAVLRKYEIFVVCDEVYSELTYTGEAH
                     VSLGTMLRDQAIIINGLSKSHAMTGWRLGLIFAPAAFTAQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            3649..4659
                     /locus_tag="VVE42_001619"
     CDS             3649..4659
                     /locus_tag="VVE42_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEU3073790.1"
                     /translation="MSFLSKNGAGILVCLLISIVSWYLGGFFPVIGAPVFAIFMGMLL
                     HPFLSSYKQLDVGLTFSSKKLLQYAVILLGFGLNISQVFAVGQSSLPVILSTISIALI
                     VAYLFQRFFALDTKLATLIGVGSSICGGSAIAATAPVIHAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNDGFALFAGTAVNDTSSVTATASAWDSLYQTNTLESATIVKLT
                     RTLAIIPITLFLSYWQSREQKNKQGLQLKKVFPLFILHFILASLLTTLLTSLGVSSSF
                     FTPLKQLSKFLIIMAMSAIGLKTNLVAMVKSSGKSIVLGAVCWIAIILTSLGMQTLIG
                     IF"
     gene            complement(4862..4942)
                     /locus_tag="VVE42_001620"
                     /pseudo
     CDS             complement(4862..4942)
                     /locus_tag="VVE42_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(4977..5414)
                     /locus_tag="VVE42_001621"
     CDS             complement(4977..5414)
                     /locus_tag="VVE42_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEU3073791.1"
                     /translation="MSQEFINPSDGVIRQYLATSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLEAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
     gene            complement(5499..8132)
                     /gene="polA"
                     /locus_tag="VVE42_001622"
     CDS             complement(5499..8132)
                     /gene="polA"
                     /locus_tag="VVE42_001622"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEU3073792.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNAAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDH
                     MGIRHYELAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEHGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNVSSADVSESLDFTIVDQISQDMLSEESIFHFELFGENYHTDNL
                     VGFAWSCEDKLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQPYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVETEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIVVKKETLLEMQAENEL
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYLQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDNVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPGIKNYMDEVVREARDKGYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEMKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
BASE COUNT         2266 a         2107 c         1513 g         2348 t
ORIGIN      
        1 tagaatccta gttcatgatt gataatacca gcaatcaaat tcattcgtaa tccgaagcgt
       61 ttacgatgat ttcgataggt tgttgaaaac attttaaacg tttttacttt ggcaaagata
      121 ttctcaacct tgcttctctc cttagatagc gcatgattac aggctttatc ttcaactgtt
      181 agcggcttga gtttgctgga tttacgtgga gtttgtgctt gaggatatat cttcatgagc
      241 ccttgataac cactgtcagc caagatttta ccagcttgtc cgatatttct catttctaaa
      301 aaccatctac ttccgttgac ttggaactcc gattgctatt ttccttgaat gattttaacc
      361 acatctccta cgttttggag ttggtcaatt tcttcatcgc tgatttcgat actaaattca
      421 tcttccagag tcaagataaa ctccatcaag tcaacagaat ccgcatccaa atcgtctttc
      481 agactcaagg attctgtcac gacaaagtcc tctccctgtc gctcttggat aatggtcaca
      541 atacggtcaa aaatttcttt ttctgtcatc tcttttattc tcctgaaaat tcacgcgcag
      601 tctgggcaac tacgtctgtt tctagcatgg tacgaatctg gcgaatcgta ctataaacag
      661 ccttggcatc gcttgagcca tgagtcttga caacaggtgc cttaacacca aacaagaccg
      721 ctccaccaac atctgaataa ttgagctgtt ttttcaaacc tctgaggctg tccttgagaa
      781 ggagggcacc tagtttcgct cgaagaccac cacctgtaat agctgtcttg agcaagccca
      841 tgattcccat agctgtccct tcgatggatt tgagcacagc gtttcccgtg aaaccatctg
      901 ccacaacaac atctgcaaca ccattcatca aatcacgcgc ttccacattt ccgataaagt
      961 tcaaactttc atcagccact agtaattcat aagtttcctt acgaagcgga tcgcccttgc
     1021 tactctctgt tccgttattg agcaagccaa cacgtggttg cgcaatgcca cgaacatttt
     1081 tagcatagaa ggatcctaga accgcgtatt gatggaggtg ctgggctgta ttttccgcat
     1141 tggcaccgag gtctagcatg tcaaatccct tcccatctac agtcggcaaa gtcgacataa
     1201 gcccaggacg gtcgatattc ttgatacgac ccacgatgaa gaatcctgct gccaacaagg
     1261 cacctgtatt cccagccgaa aggacagcgt ctgcttcacc atctttgaca gccttggctg
     1321 ccaataccat actggcattt ttcttattcc taatagctct cgtaggttca tcatccgaat
     1381 caatcttctc atccgtatgg ataatgctga cgcgctctgt cgctgtcaga tattgcttga
     1441 ttttagcttc atctccgtaa agttgaacct cgatatctga aaagtcagat agggcttgat
     1501 tgacaccctc aacaatggcc tgaggtgcgt aatcgccccc catggcatct actgcgattt
     1561 ttttcatttc ttttcctctt ttagtaattg tccccagtct gctagggaat caataaattt
     1621 ctttgatttt aggtgaatcc caacgtactc ttcatataat tgatccataa attggcgtag
     1681 ctcttgcttg attccaggct tgagcgaaat ggtctccaaa gtctcaaaat caatagcttg
     1741 aaattgattg agcagatagg ggatattggg attgagatga caacgtctct tatcctcatg
     1801 ataatgctct ggacagaggc aggctccata tttgaaagaa aagtcaaaag cctgaccaac
     1861 ccgatggcag aagacacact cattaaaatt gaggctgatt ccaaatcgag tcaaaatttg
     1921 aatttcaaaa atattggtca aaacctgata atccaagcct gcttccatca actccaaagt
     1981 cttttgcaaa aaagcaaaca agggagcatc ctgctgattg tcctgcaaac tagcatctgc
     2041 aagagctgcc acataggtcg cataggccat gacaaagagg tcactattaa tcttgggaaa
     2101 agtcatgacc tcatgatagt cttcgatgta actgagtccg tcatcattga ttcgcaagag
     2161 aaatcgtgcc agcaccaagg gctgaataac aggcgccagc ttagactgac cagcgtgttt
     2221 gacaaaaaac atgcgtttgc caacctgctc tgtaaaaatt ttgacgagct tgtcatcctc
     2281 acgaaaattg cgattgtaaa gcaccaagcc ttgactcgtg atagactgaa tcatgcttct
     2341 ctcatgtact cctcaagtcg tttcatggct tctttgatag tctccatgct ggctgcataa
     2401 gataggcgga cgtagccttc cccgtaacgt ccaaaggctg caccagggat aaaggcaacg
     2461 gccttcttct gagcaaaatc cttcagaaaa gcaaaggagt cttgattgta gcccgctgga
     2521 attttagcaa aaatatagaa ggcaccgtct ggtttgataa tctcaaaacc aagagcagtc
     2581 attttttcga tgatatagtc ccgacgctgg atatattcct tcttcatggg ctccgcatca
     2641 tttttaccag ccgtcaaggc ttctaccgca gcatgttgcg ccatggtatt tgcggcagtg
     2701 accaagtatt ggtgactctt gattaactgg gctgtgaagg ccgcaggagc gaaaatcagc
     2761 cccaaacgcc aacctgtcat ggcatgcgat ttagacaaac cattgataat aatagcctgg
     2821 tctctcaaca tcgttcctag agacacatgg gcttcgcctg tgtaggtcaa ttctgagtaa
     2881 acctcatcac agacaacaaa aatttcgtac ttgcgtaaaa cagctgccaa ggcctctaac
     2941 tgctctcgac tgtaggtaat tcctgtcgga ttggctggat agttgagaat aaccgccttg
     3001 agcttatcac cctgctccaa aatggccttc tccaacatct caggagtcaa gacaaaacca
     3061 ttttcagtcg tatcaatctc aacaatttct gccccaacta agttaacaat cggttcatag
     3121 cctggataag caggagctgg caaaagtacc ttgtctccct cttccaaaat agccgtcaaa
     3181 gtcgcagata aagcctctgt cgccccaatt gtaaccaaga tttcattttc aggagcatag
     3241 tccagttggt acttttcctt aacaaagtca ctggctgcct gacgtagagt cagcagacca
     3301 ctcatccctg tatagtagga ttggttctga tcaatcgctc gcttggccgc ctccttgaca
     3361 tggtctggcg ttgtaaaatc aggttccccc aaggtcaaac gcaagacccc aggaatctcc
     3421 gaaataacct ggtcaaactg acgaatcaac gaaacttgaa ttttatctaa ctgtttatta
     3481 aagcgcttag ttaagtccat agatacctcc tactttcaaa acgtatctct attatactac
     3541 aaaagccccc cgaccgcaaa aatacattag agaatagctt tccactttct attttacttt
     3601 tcctgtttac aggtctataa tggtaacaga ttctatttgg aggtttttat gtcatttcta
     3661 tcaaaaaatg gagcaggcat cttggtctgc cttctcattt ctatcgtatc ttggtactta
     3721 ggaggattct tccctgtgat tggcgcgccc gtttttgcga tttttatggg catgctccta
     3781 catccctttc tctcgtctta taaacaactg gatgttggat tgacctttag ttccaagaaa
     3841 ttgctccagt atgccgttat cttgcttggt tttggtctca atatctctca agtcttcgca
     3901 gttgggcaat cttcactccc tgtcatcctt tccaccattt caatagcctt gattgttgcc
     3961 tacctcttcc agcgcttctt tgccctggat acaaaactgg ctaccttgat tggagtaggt
     4021 tcttctatct gtgggggttc tgccattgca gcgacagcgc ctgttatcca tgctaaggaa
     4081 aaggaagtag cccaagccat ctccgttatc tttttcttca atgtcttggc tgcgctcatc
     4141 tttccaaccc tcggcacctg gcttcatcta tccaatgacg gcttcgccct ctttgcagga
     4201 actgcggtca atgatacttc ctctgtaacc gccacagcta gcgcttggga cagtctctac
     4261 cagaccaata ccctcgaatc cgcaaccatt gtcaaactca cgcgcaccct agctatcatc
     4321 cccatcactc tctttctctc ctactggcaa agtcgcgagc aaaaaaataa gcaaggctta
     4381 caactgaaaa aagtcttccc actttttatc cttcacttca tccttgcatc gctcctaacc
     4441 acccttctga cctctctcgg tgtgtctagt agtttcttta cccctctcaa acaactctct
     4501 aaattcctca ttatcatggc catgagtgcc atcggtctca aaactaatct ggtcgctatg
     4561 gtcaaatcca gtggaaaatc cattgttctc ggagccgtct gctggattgc catcatcctc
     4621 accagccttg gtatgcagac ccttatcggc attttctaat actcttcgaa aatctcttca
     4681 aaccacgtca acgtcgcctt gccgtatata tgttactgac ttcgtcagtt ctatctgcaa
     4741 cctcaaaacg gtgttttgag ctgacttcgt cagttctatc tgcaacctca aaacggtgtt
     4801 ttgatctgac ttcgtcagtt ctatctgcaa cctcaaaacg gtgttttgag ctgacttcgt
     4861 cagttctatc tacaacctca aaacagtgtt ttgagcagcc cgcggctagt ttcctagttt
     4921 gctctttgat tttcattgag tataacacaa aaggtagccc atcagctacc tttttcttat
     4981 gcttcctcaa tcaagcgagt atgttctctc ttgatacagc gattcatcac gatatcatca
     5041 catccaccat cacgcaaaat ctctttcgct tctaaacttt caagtcctag ctgtgcccaa
     5101 aaaatcttgg catcagcttt gagaaaatca cgcgccacat cgggcagaaa ttcactgcga
     5161 cgataaacat tgacaatatc tacaggaaaa ggaatttcag cgaggctagc ataagccttt
     5221 tcacccaaga tttcgccacc tgccgccttg ggattgactg ggatgatttt atagccccga
     5281 gcctgcattt cctttgttac tcgattgctg gttgtttctt cacggtcaga caaacccacc
     5341 acagcaaggg ttttactcgt tgcgagatac tgacgaatca cgccatcact tggattgata
     5401 aattcttgac tcatagaaat cctccttttt catcagtata gcacattttg aaaaggtttg
     5461 cagaattcta ctacaaaaaa ggaggactag cccccttttt atttagcctc gtaccaggtt
     5521 gccccttcat tctcatctgc gataagagga acactgagtt gaatggcttc ttccatggtt
     5581 tgtttcacca attttttcat ctctaccaat tcagatttag gcacttcaag gacgatttca
     5641 tcgtgcactt gtaacagcat cttagtctga taaccacctg caaccaaggc tttatccagc
     5701 tgaatcatgg caatcttgag aatatctgct gccgaaccct ggataggtga gttgatagca
     5761 gttcgctccg caaaaccacg aatattgaag ttgcgcgaat tgatatctgg caactcacgg
     5821 cgacgcttaa agagggtctc tacatagccc ttatcacgcg cctcccgcac cacttcatcc
     5881 atgtagtttt taatacctgg aaaacgttca aagtaggtat caatgtaggc tttggcttcc
     5941 ttacgactaa ttcccaaatt attagacaag ccaaagtctg aaatcccata aaccactcca
     6001 aagttaactg ccttggcatt gcgacggtca tttgcagtca cattatcagg acgctcaatg
     6061 ccaaagaccc gcatggctgt cgaagtatgg atatctgccc cctcttggaa ggccttaatc
     6121 aagtgctcat ccttagaaat atgcgccaaa acgcgcaatt caatctgtga atagtcagag
     6181 ctgagtagca cactatcctc ccactctggc acaaaagcct tccgaatcaa gcgcccttgt
     6241 tccaatcggg caggaatatt ttgcaagttt ggatccacac tagacaaacg cccggtctgg
     6301 gtcaaatcct gcaaatagcg agtatgaatc tttccatcag ccaaaatcca gtcctgcaag
     6361 ccaattacat aagtagattg aatcttagca atttgacggt aatccaggat tttcttaaca
     6421 atcggagcaa taggagcgag acgctccaac acatccactg ctgtcgaata acctgtcttg
     6481 gttttcttag tgtattctag aggaagtccc aatttctcaa agagaagcac gcccaactgc
     6541 ttaggcgagt tgacattaaa ctcctcacca gccagctcgt aaatctcttg agtcagtttt
     6601 tcaatgacaa gctcattttc agcctgcatc tcaagcaagg tctctttctt gaccacaatc
     6661 ccagcaattt ccatcttggc aaggacaaaa gccagaggtt gctccatatc ataaagaagc
     6721 tctaattgcc cattttcgct gagtttttca agtaaaatag gctctgtttc taccaaaaca
     6781 gcaagtttac aagctaagtg ttccaagaat ttctcacgtt caggaatggc ctttttaaca
     6841 cccttaccgt agaaagtttc atcatcaacc aagtaaggct gaccataaag actagcgatg
     6901 gtcgcaattt cattgtcctc cacagtcgaa aggaggtatt tagccaaacg gatgtcaaaa
     6961 gcaggcgcct gcaaatccac accaaaacgt tgcaaaagaa ctttaacctt cttaaagtca
     7021 taaactctca gagatgtttt ttctaagaaa tccttgaaaa tcgggtcttg caacagctca
     7081 agcttgtctg tggcatagag cttatcctca caagaccagg caaatccaac caaattatcc
     7141 gtatggtaat tctcaccaaa aagctcaaag tggaagatag actcttcact cagcatatct
     7201 tgactgattt ggtcaacaat agtaaaatcc aaactctcag acacatcagc tgacgacaca
     7261 tttaaagcct gctttagctg tttgaagccc atctcatcgt agaatttccc aagattttca
     7321 acatctggac cactatagac caagtcctct aaaccaatcg caatcggtgc cttggtatca
     7381 atggtcgcta gtgttttaga caaaaaggcc tgttccttgt cattgatgag attttccttc
     7441 atcttagaag tcttcattcc atcaatattt tcataaatcc cctcaagcga accatgctcc
     7501 agcaagagct taatacccgt cttttcaccg actttggtca ccccagggat attatccgac
     7561 ttatcaccca tgagcgcctt gagatcgata aactgagctg gtgtgaggcc catttcttcc
     7621 atgaggtaat ctggcgtaaa ggcctcaaac tcagccacac ctttcttgga aatttcaacc
     7681 accgtatgct catccgtcag ctgaatcaaa tccttgtccc cactgacaat ggtaatatca
     7741 aaaccatcct gctctgctag cttatccagc gtcccaatga tgtcatccgc ctcatactga
     7801 gccagctcat agtgacgaat ccccatatga tccagcaact cacgaatgaa aggaaattgc
     7861 tcacgaaact catcaggagt cttggcccga ccacccttat agtccgcata catctctgtc
     7921 cggaaggtcg tctttcccgc atcaaaagcc accaaaatat gactcggctc aacccgctcc
     7981 aacaaatgac tcaacatcaa ctgaaaacca taaatcgcat tggtatgcaa accagccgca
     8041 ttcttaaaac ggtccaactg ctgatacagc gcaaaaaacg cccgaaaagc tacagaagac
     8101 ccatcaatca ataataattt tttcttatcc atacacccat tataaaggaa agaatcaaaa
     8161 aataccattg ggaagagcta gagcaagtat ttttcaaact ttttccgaat aaatagatag
     8221 agccagagaa ttta
//
LOCUS       DAVGJL010000095         7990 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.096, whole genome shotgun sequence.
ACCESSION   DAVGJL010000095 DAVGJL010000000
VERSION     DAVGJL010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7990
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.096"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            25..585
                     /locus_tag="VVE42_001623"
     CDS             25..585
                     /locus_tag="VVE42_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HEU3073793.1"
                     /translation="MQKFIQAYIEKLDVTTIIENILTKVISLLLLLIVFYIAKKMLHT
                     MVQRIVKPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGA
                     GIAGVAIGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQ
                     LRSEEQALHFVPNRNITVVSNFSRTD"
     gene            642..1550
                     /locus_tag="VVE42_001624"
     CDS             642..1550
                     /locus_tag="VVE42_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEU3073794.1"
                     /translation="MVLEKQLGNGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESGTVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIE
                     QMTRYLESHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFA
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDEPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            1502..2092
                     /locus_tag="VVE42_001625"
     CDS             1502..2092
                     /locus_tag="VVE42_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEU3073795.1"
                     /translation="MDCFILVTKENCEKKLRKGARMAIENYIPDFAVEAVYDLTVPSL
                     QAQGIKAVLVDLDNTLIAWNNPDGTPEMKQWLHDLRDAGIGIIVVSNNTKKRVQRAVE
                     KFGIDYVYWALKPFTFGIDRAMKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKP
                     LVQHDSIKTQINRTRERRVMRKITEKYGPITYKKGI"
     gene            2095..3201
                     /gene="yqeH"
                     /locus_tag="VVE42_001626"
     CDS             2095..3201
                     /gene="yqeH"
                     /locus_tag="VVE42_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEU3073796.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTNDDFLKLLHEVGDSDALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAYEEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFIAGEKQ
                     GFTAFFDNELKLHRSKLEGASAFYDKHLGTLLTPPNSKEKEDFPRLVQHVFTIKDKTD
                     LVISGLGWIRVTGTAKVAVWAPEGVAVVTRKAII"
     gene            3415..3726
                     /gene="yhbY"
                     /locus_tag="VVE42_001627"
     CDS             3415..3726
                     /gene="yhbY"
                     /locus_tag="VVE42_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEU3073797.1"
                     /translation="MSLTSKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            3778..4407
                     /locus_tag="VVE42_001628"
     CDS             3778..4407
                     /locus_tag="VVE42_001628"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEU3073798.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLVPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEQHPDTDYYFIIGADMVDYLPKWYRIDELVDMVQFVGVQRPRYKA
                     GTSYPVIWVDVPLMDISSSMVRDFIAQGRKPNFLLPQPVLDYIEKEGLY"
     gene            4407..5000
                     /gene="yqeK"
                     /locus_tag="VVE42_001629"
     CDS             4407..5000
                     /gene="yqeK"
                     /locus_tag="VVE42_001629"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEU3073799.1"
                     /translation="MSYQDYINCSREALLEKMAELLPEKRLTHCLGVERAAMELAQRF
                     GVDVEKASLAGLLHDYAKKLSDQEFLDLIDRYQLDPGLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEVHTVGAGQMTDLDKVVYVADYIEHNRSFPGVDVAREVASLSLNKA
                     VAYETARTVEYLAHQGFPIYPQTLETYNAFVHYLKEN"
     gene            5001..5501
                     /locus_tag="VVE42_001630"
     CDS             5001..5501
                     /locus_tag="VVE42_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEU3073800.1"
                     /translation="MKMAFIIIDVQNVLVETGFQTKSLLEKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEDWQLSALLNRKPAEKVFQKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKIAFEYGYQLIVPEGAVTTFDGDDIPAETINEFYEDIWEERFADVL
                     DYKHIF"
     gene            5514..5867
                     /gene="rsfS"
                     /locus_tag="VVE42_001631"
     CDS             5514..5867
                     /gene="rsfS"
                     /locus_tag="VVE42_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEU3073801.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYFVITSSMN
                     SRQLDAIAANIREKVAQAGFKGSHVEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEANSVDISEALA"
     gene            5970..6716
                     /locus_tag="VVE42_001632"
     CDS             5970..6716
                     /locus_tag="VVE42_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU3073802.1"
                     /translation="MIMATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKERKKLLE
                     LACGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKIAFIEGNMLNLSKAGK
                     YDFVTCYSDSICYMQDEVEVGDVFKDVYNVLNEEGVFIFDVHSTYQTDEVFPGYSYHE
                     NAEDFAMLWDTYEDEVPHSIVHELTFFIKEADGSFSRHDEVHEERTYEILTYEILLEQ
                     AGFKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            6726..7823
                     /locus_tag="VVE42_001633"
     CDS             6726..7823
                     /locus_tag="VVE42_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEU3073803.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLDQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRTQMALENGADLVVELPFLVSVQAADFFGQGAVDILDRLGIDSLVFGTEEVLD
                     YQKIADLYTEKGAEMEKFVENLPDSLSYPQKTQAMWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGRNIKLHPIQRQGAGYHSVNKDVDFASATALRQHQKDQDFLERFMPSVALFEQA
                     SKVIWEDYFPLLRYQILSNPDLTTIYQVNQEMAVRIKEAIKTAQSVEELVELVTTKRY
                     TKARVRRLLTYILMQARESDLPEAIHVLGFTEKGRQHLKSLKGQVSLVSRIGKEPWDA
                     MTQKADQIYQLGKPSIAEQNFGRVPIRIETN"
BASE COUNT         2389 a         1580 c         1833 g         2188 t
ORIGIN      
        1 tataatgaaa taaaggagtt ttatatgcaa aaatttattc aggcttatat tgaaaagcta
       61 gatgtgacaa ccattatcga gaatattcta accaaggtca tttctctttt actgcttttg
      121 attgtatttt atattgctaa aaaaatgctt cataccatgg tgcagagaat tgtcaaacct
      181 tctctaaaaa tgtctcgtca tgatgtcgga cgccaaaaaa ccatctcacg tttactagaa
      241 aatgtgttta attatacgct atatttcttt ttactctact gcattttgtc gattttaggt
      301 ttgccagttt ctagtttgct ggctggagct ggtattgctg gggtagcgat tggtatggga
      361 gcccaaggct ttctgtctga tgtcatcaat ggctttttca tcctctttga acgtcaactg
      421 gatgtgggag atgaggtcgt tctgacaaat ggaccgatta ctgtatcggg taaggttgtc
      481 agtgtgggaa ttcgtacgac acagcttcgt agcgaggagc aagcccttca ctttgtccct
      541 aaccgaaata tcacagttgt tagcaatttc tcacgcacag actagacctg ttattttaag
      601 taatttgtgg tacaatagag ggagtttaat aaggagaaaa gatggtttta gaaaagcagt
      661 tgggcaatgg ttgtacctgg atagacctag acctaggaaa gttgaataaa ctagaagacc
      721 tttctgaaat ttacggtttg gacaaggaaa ccattgaata cgcactggat agaaacgagc
      781 gcgcccacat ggactaccac cgtgaaagtg ggacggttac ctttatctat aatgtcctag
      841 atgtaaaaaa ggacaaggcc tactatgaga cttttcccat gacctttatt gtcgagcatc
      901 gtcgcctgat taccattagt aataccaaga acgcctatgt cattgaacag atgactcgtt
      961 atctggagag ccatgacacg ctttcgattt ataagtttct ctttgccagt ctggaaatta
     1021 tcagcaatgc ctactatcct gtcattgagc agatggacaa gagtagggat gaggtcaatg
     1081 acctcttgcg ccagcgaact accaagaaaa acctctttgc cctgtctgat ttggagactg
     1141 gtatggttta tctgacggca gctgccaaac aaaatcggat tttgttagag catattcaag
     1201 gtcatgcctt gtatcgtagt tttgatgaga ttgagagaga acagtttgat gatgccatga
     1261 ttgaggctca tcagctggta tccatgacag acctaatctc tcagatttta cagcagcttt
     1321 cagcctctta caacaatatt ctaaacaata atctgaatga caatttgaca accttgacta
     1381 tcatttcagt cttgctagct gttttggcag tcgtgacagg ctttttcgga atgaatgttc
     1441 ccttaccttt aacagatgag ccccatgctt ggctctatat cagtttggct agtgcaggtt
     1501 tgtggattgt tttatccttg ttactaagga aaattgcgaa aaaaagttaa gaaaaggagc
     1561 cagaatggcg attgaaaatt atataccaga ttttgctgtg gaagcagtct atgatctgac
     1621 agtcccaagc ctgcaggcgc agggaatcaa ggctgttttg gtcgatttgg ataataccct
     1681 cattgcttgg aacaaccctg atggaacgcc agagatgaag caatggctac atgaccttcg
     1741 ggacgcgggt attggcatta tcgtagtgtc aaataacacc aaaaaacgcg ttcaacgagc
     1801 agttgagaaa tttgggattg attacgttta ctgggccttg aagcccttca catttggtat
     1861 tgaccgtgcc atgaaggaat tccactatga caaaaaggaa gtagtcatgg ttggcgacca
     1921 gctcatgaca gatatacgag cagcccaccg tgcagggatt cggtcaattt tagtcaaacc
     1981 cttggtccaa catgactcaa tcaaaacgca gattaaccga actcgtgagc gtcgtgttat
     2041 gagaaaaatc actgaaaagt acggaccgat tacatataaa aaaggaattt aactatggaa
     2101 gaaattctct gtattggttg tggagcaacc attcagacga cagataaagc tggtcttggt
     2161 tttacccccc agtcggcact tgaaaaaggt ttggagactg gcgaagtcta ttgccaacgc
     2221 tgtttccgtc tccgccacta caatgaaatc acagatgtcc agttgacgaa cgatgatttc
     2281 ctcaagctct tgcacgaggt gggagacagt gatgctttag tggtcaatgt cattgatatc
     2341 tttgatttta atggatctgt catcccaggt ttaccacgtt tcgtctcggg caatgatgtc
     2401 ctcttggtag gaaataaaaa agatatcctt cctaagtcag ttaagtctgg taagattagc
     2461 cagtggctca tgaaacgtgc ctatgaagaa ggtcttcgtc cagtcgatgt ggtcctaact
     2521 tcagcacaaa ataaacatgc cattaaggaa gtcattgaca agattgaaca ctaccgtaag
     2581 ggccgcgatg tctatgtggt cggtgtgacc aacgttggaa aatcaactct aatcaatgct
     2641 attatccaag aaatcacggg tgatcagaat gttatcacta cttcacgctt cccagggaca
     2701 accttggaca aaatagagat tccgcttgac gacggatctt atatttacga tacgccggga
     2761 attatccacc gccaccagat ggctcactac ttgacggcca aaaacctcaa gtatgtcagt
     2821 cctaaaaagg aaatcaagcc taagacctat cagcttaatc ctgagcaaac cctattttta
     2881 ggtggtttgg gacgctttga ctttatagca ggagaaaagc aaggatttac tgctttcttt
     2941 gataatgaac tcaaactcca tcgtagcaag cttgaaggag ctagtgcttt ctacgataag
     3001 cacctgggaa ctcttctgac accaccaaat agcaaggaaa aagaagattt cccaaggcta
     3061 gtccagcatg tctttaccat taaagataag acagacctag tcatctcagg cctaggctgg
     3121 attcgtgtaa caggcacagc aaaagtcgcc gtctgggcac cagaaggcgt cgccgtcgtt
     3181 acacgaaaag caattattta aaacaaaatc gctgagcgaa caaagtgagc tcatatagcg
     3241 atagttattg ccgtggtgtg attgccaatc tgtagattgg caaggggcac aattgagacc
     3301 aaggctcaat tgtgaggtca ggaaatccat ttttcaaaga tgaaatcttt ggaaaattag
     3361 ttccgaccgt ccctcaccac ctaaaataac tatcaaaaaa caaggaagaa aattatgtca
     3421 ttaacatcaa aacaacgtgc cttcctcaac agccaggcac acaccctcaa acctatcatc
     3481 caaatcggga aaaatggact caacgaccaa atcaaaacca gcgtccgtca agctcttgat
     3541 gcgcgtgaat taatcaaggt tactctctta caaaacacag atgaaaacat ccacgaagta
     3601 gctgaaattt tggaagaaga aatcggtgtg gatacagtcc aaaaaatagg acgcatcttg
     3661 attttgttta aacaatctag caagaaagaa aatcgcaaga tttctaagaa agtcaaagaa
     3721 atctaagacc gaaactccaa ataactgttt ttatagagaa ataaagggga ctagcctatg
     3781 acaatcgaac tattgactcc ctttaccaag gtagagttgg agccagaaat caaggagaaa
     3841 aaacgcaaac aagttgggat tttagggggg aattttaacc ctgttcacaa tgcccatctc
     3901 attgttgcgg atcaagtacg gcaacagttg ggactggatc aagttcttct cgtgcctgaa
     3961 taccaacctc ctcacgttga taaaaaggaa accatccctg aacaccatcg tctcaagatg
     4021 cttgagttgg caattgaggg gattgacggc ctagtcattg aaaccattga gttggagcgc
     4081 aagggtattt cctacaccta cgacaccatg aaaatcttga cagagcaaca tccagatacg
     4141 gattattact ttatcatcgg cgccgacatg gttgactatc tgcctaagtg gtaccgaatt
     4201 gatgaactgg ttgacatggt tcagtttgtg ggggttcagc gtccacgcta caaggcaggg
     4261 acttcctatc cagttatctg ggtggacgta ccgctcatgg atatctcgtc cagcatggtg
     4321 cgggacttca ttgcccaagg tcggaaaccc aactttctcc tacctcagcc agtgctagac
     4381 tacatcgaga aggaggggct ctactgatgt cctatcaaga ctatatcaac tgctcccgtg
     4441 aggccttgtt ggaaaaaatg gcagagcttc tacctgagaa acgtctaacc cattgtctag
     4501 gtgtggagcg tgccgctatg gaattagctc agcgctttgg tgtagatgtt gagaaagcaa
     4561 gtctagcagg tcttcttcat gactatgcta aaaagttgtc agatcaggaa tttctagact
     4621 tgattgaccg ttaccagcta gaccctggcc tcaaaaactg gggcaataat gtctggcatg
     4681 gtatggtcgg aatttacaaa attcaggaag atttggattt gcatgattca gaaatcctgc
     4741 gagccattga agtccataca gtcggtgctg gtcagatgac agacctagat aaggttgtct
     4801 atgtagcaga ctatatcgag cacaatcgat cctttcctgg agtagatgtg gcgcgtgagg
     4861 ttgcaagtct atcgctcaat aaggcggtgg cctacgaaac agctcgtacc gtggagtatc
     4921 tagctcatca gggattcccc atctatcccc aaacccttga aacctataac gcctttgtgc
     4981 actatttgaa agagaactaa atgaagatgg cttttataat cattgatgtt cagaatgttt
     5041 tagtagaaac tgggttccag acaaaaagtc tattggaaaa aatttcttat ttacaaaacc
     5101 aggctagaag caagaatatt gaaattatct atgttcaaca tattgagaac tctgaagctc
     5161 aaacatcaga agattggcag ttatctgcgc ttttaaatcg aaaacctgct gaaaaggtct
     5221 ttcagaagaa atataacagt attttcaaag aaactggctt aaaagaatac ttggataagc
     5281 aggggattga aaaattagtt ttatgtggta tgcagacaga atattgtgtg gatacctctg
     5341 tcaagattgc ctttgaatat ggctatcagc ttattgttcc agaaggtgct gtcacaacct
     5401 ttgatgggga tgacattcca gcagaaacga ttaatgaatt ttatgaggac atttgggagg
     5461 agcgctttgc agatgtccta gattacaaac atattttcta aaaagaggat taaatgaacg
     5521 aaaaagaatt actagaacta gtcgtgaaag cggctgatga gaaacgtgcg gaggatatcc
     5581 tcgcacttga tgtacaagat ttgactagtg tgacggacta ctttgtcatc actagctcaa
     5641 tgaatagccg tcagctggac gctatcgcag ctaatatccg tgaaaaagta gctcaagcag
     5701 gctttaaagg tagccatgtc gaaggcgatg cagctggagg ctgggtctta ctggacctcg
     5761 gtgctgttgt cgtgcatatc ttttcagaag aaatgcgtgc ccattataat ctagagaagc
     5821 tatggcatga ggcgaattca gtagatattt cagaagctct tgcttagaag atgaactcag
     5881 ttgtagtcaa ctgggtttct ttgcttattt tagaaaaatt gatagaaagg taaagggcta
     5941 gtggtgttta ccatggagct cttagtatca tgattatggc gacttatgaa acctttgcgg
     6001 cggtctatga tgcggtcatg gacgatagtt tatacgacaa atggacgaat ttttctctgc
     6061 gtcatttgcc taagaccaag gagagaaaga aactcttgga attggcttgt gggacaggaa
     6121 ttcaatcagt gcgcttctct caggctggtt ttgatgtgac tggacttgac ttgagtgcgg
     6181 atatgttgaa gattgcggag aagagagcaa cttcagccaa gcaaaagatt gcttttatag
     6241 aaggtaatat gctgaatttg tccaaggcag ggaaatacga ttttgtcacg tgttattcgg
     6301 actctatctg ctatatgcag gatgaggtgg aagtagggga cgtctttaag gacgtgtaca
     6361 atgtgctgaa tgaagaggga gttttcatct ttgacgtgca ctcgacctac cagacagatg
     6421 aagtattccc tggctattcc taccatgaaa atgcggaaga ttttgccatg ctttgggata
     6481 catatgagga cgaagttcct cactccatcg tgcatgagct gactttcttt atcaaggagg
     6541 ctgacggttc ctttagtcgc cacgatgaag tacatgagga gaggacctat gagatcttga
     6601 cctatgagat cttgctggaa caggctggat tcaagtcctt caaactctat gcagactttg
     6661 aggataagga gccaacagaa actagcaccc gttggttttt tgtggcgcag aagtaggaga
     6721 ttagcatgac catcacaggt attatcgcgg agttcaatcc ttttcataat ggccataaat
     6781 acctgctgga tcaggcagag ggactgaaaa ttgttgccat gtcagggaat ttcatgcagc
     6841 gtggagagcc tgctattgtg gataagtgga caaggaccca gatggcgctg gaaaatggcg
     6901 cagacttggt agtggaattg ccctttttag tcagtgttca ggcagctgat ttctttggtc
     6961 aaggagctgt ggatatcttg gatcgcttgg gcattgatag tctagttttt gggacagagg
     7021 aagttctgga ttaccagaaa atcgctgact tatacacaga gaaaggtgct gagatggaga
     7081 aatttgtgga aaatctgcct gattcccttt cctatcccca gaaaacccaa gctatgtgga
     7141 aggaatttgc tggtcttgat ttttcaggta atacccccaa tcatgttctt gctctggcct
     7201 atgccaaggc ggttgcagga cggaatatca agctgcatcc gattcagcgt cagggggcag
     7261 gttaccattc tgtgaataaa gatgtggact ttgcctcggc gacagccctc cgtcaacatc
     7321 agaaggacca agatttctta gaacgtttta tgccttctgt tgccctattt gagcaggcca
     7381 gtaaggtgat ctgggaagac tattttccct tgctccgcta tcaaatcttg tcaaatcccg
     7441 acctaaccac catctatcag gtcaatcaag aaatggctgt gcgcattaaa gaagctatta
     7501 aaacagccca gtctgtggaa gaattggtcg agttagtgac taccaaacgt tacaccaagg
     7561 cgcgtgtcag acgcctattg acctatattt tgatgcaggc tagagaaagt gacttgccag
     7621 aagccattca tgtccttgga tttactgaaa aaggtaggca acatctcaag tctttgaaag
     7681 gtcaggtcag tctagtcagc cgaattggca aagaaccttg ggatgctatg acccaaaaag
     7741 cagaccagat ttaccaacta gggaagccaa gtatagcaga gcaaaatttt ggcagagtgc
     7801 cgattagaat agaaacaaac taagtctacc gaaaggtagg cttttttatt gtagaaattt
     7861 tagataacaa attgaagata aagtttttgg aaaatgataa actccctaat acagaagaca
     7921 aaaagtcaat aaagaaaatt agtatatgaa ccttaatctt tatctttgaa tccgctttat
     7981 gcttgttatt
//
LOCUS       DAVGJL010000096         7921 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.097, whole genome shotgun sequence.
ACCESSION   DAVGJL010000096 DAVGJL010000000
VERSION     DAVGJL010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7921
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.097"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(<1..192)
                     /locus_tag="VVE42_001634"
     CDS             complement(<1..192)
                     /locus_tag="VVE42_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073804.1"
                     /translation="MKKTVYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSET
                     LQDSPGTNNFSLPNVSPTYG"
     gene            complement(342..608)
                     /locus_tag="VVE42_001635"
                     /pseudo
     CDS             complement(342..608)
                     /locus_tag="VVE42_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(633..944)
                     /locus_tag="VVE42_001636"
     CDS             complement(633..944)
                     /locus_tag="VVE42_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEU3073805.1"
                     /translation="MDWDFADTCWEKEPRKYQYVAANYLKAMQSYLKETDLPKLEQLV
                     VTKSWWDTVDILDRVVGSLVYEHPELEEIILILFENLFKPRQLYLQPQNSVLSNLRLA
                     S"
     gene            complement(869..1111)
                     /locus_tag="VVE42_001637"
                     /pseudo
     CDS             complement(869..1111)
                     /locus_tag="VVE42_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812384.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(1112..1744)
                     /locus_tag="VVE42_001638"
     CDS             complement(1112..1744)
                     /locus_tag="VVE42_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU3073806.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGHEEF"
     gene            complement(1758..2753)
                     /locus_tag="VVE42_001639"
     CDS             complement(1758..2753)
                     /locus_tag="VVE42_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU3073807.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLHDVEKTEK
                     FVFLLLVFSMSITCLLALFWRGIEELSLRKMQANLKRLLAGQEVVQVADPDLDASFKS
                     LSGKLNLLTEALQKAENHSLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQ
                     QALKLDREKMQTQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFQLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            complement(2750..3448)
                     /locus_tag="VVE42_001640"
     CDS             complement(2750..3448)
                     /locus_tag="VVE42_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEU3073808.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAATILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KMVGTTKIIVPVDVEVSLSVNCLYGDLTFFNQPKRALRNEHYHQETKDYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            complement(3526..4536)
                     /locus_tag="VVE42_001641"
     CDS             complement(3526..4536)
                     /locus_tag="VVE42_001641"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEU3073809.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAETCGILFVTGSYSAALKNPTDDS
                     FSVKSSHPNLLLGTNIGLDKPVELGLQTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSKQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SGQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKAVGLSRT
                     VLELVETYTVEEVIGIVQGWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEANDQMK
                     KA"
     gene            complement(4520..5527)
                     /locus_tag="VVE42_001642"
     CDS             complement(4520..5527)
                     /locus_tag="VVE42_001642"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEU3073810.1"
                     /translation="MIAVKTCGKLYWAGEYAILEPGQLALIKDIPIYMRAEIAFSDSY
                     RIYSDMFDFAVDLRPNPDYSLIQETIALMGDFLAVRGQNLRPFSLEICGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYDVSVDQELLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     VYQSFDRQKVAAWLEEENLATVLERDWGFSISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLISSKETVVSLVEALEQGKSEKIIEQVEVASKLLEGLSTDIYTPLLRQ
                     LKEASQDLQAVAKSSGAGGGDCGIALSFDAQSTKTLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            complement(5514..6467)
                     /gene="mvaD"
                     /locus_tag="VVE42_001643"
     CDS             complement(5514..6467)
                     /gene="mvaD"
                     /locus_tag="VVE42_001643"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEU3073811.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEMVPATSSISLTLENMYT
                     ETTLSPLPANVTADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YPVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMDFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEHLSEIFGQRYRLIVSKTKDLSQDDCC"
     gene            complement(6449..7327)
                     /gene="mvk"
                     /locus_tag="VVE42_001644"
     CDS             complement(6449..7327)
                     /gene="mvk"
                     /locus_tag="VVE42_001644"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEU3073812.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVP
                     AESPWRLYEEDTLSMAVYASLEYLNITEACIRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQADLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSLEADSLVETTLSHGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            complement(7449..7778)
                     /locus_tag="VVE42_001645"
     CDS             complement(7449..7778)
                     /locus_tag="VVE42_001645"
                     /inference="COORDINATES: protein motif:HMM:NF024736.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU3073813.1"
                     /translation="MTRKAYDTDLNDQEWAKIEPYFSKHRTYKWPKRVLVNETLYVTK
                     TGCQWRMLPHDFPLYLTVWSFFRRSMTTGWFQVNGRWYYAYSSGALAVNTTVDGYSVN
                     YNGEWVQ"
BASE COUNT         2283 a         1880 c         1465 g         2293 t
ORIGIN      
        1 accataagtc ggtgaaacat tgggaagtga aaaattatta gttcctggac tatcttgtaa
       61 tgtctcactt acctcatatt cttcacctgt actagaaata acactcaaag cagatactgt
      121 cgataactgg ctagccaata aagtactcgc aataattgaa atacccaatt ttttataaac
      181 agttttcttc attattgtat cctcctaatg taattatagc gtactattct aaatttctta
      241 atctactata gaatcaagaa atctaccacc ttctttaaat accctccatt atcacataaa
      301 caggtaaact tttcaattaa tgactgcgct tttcaatcac gctagaggta cttacttgct
      361 tctttgatac taagttcagc cattctttcc ttggttttct caataaagca tgttacccaa
      421 gtgggattcg ttttggagta gtctcgcaga gtccagccaa tggctttatt gataaaaaat
      481 tctgtttggt tcaagttatg aagtagaatc ttttccatta attgagtatt ggtcttctct
      541 tttcttaaca actggtggtc aatagcgaca cgtctcagcc agatattatc tgataggctc
      601 cattttatac tcaatgaaaa tcaaagagca aactaggaag ctagccgcag attgctcaaa
      661 acactgtttt gaggttgcag atagagctga cgtggtttga agagattttc gaagagtatt
      721 aagattattt cttctagttc agggtgttca tacaccaaac tccctactac tcgatctagg
      781 atatctaccg tgtcccacca ggattttgtc acgactaact gctctagctt aggcaaatcg
      841 gtttccttta gataagactg cattgctttc aaatagttag cagccacata ttggtatttt
      901 ctaggctcct tttcccagca agtgtctgca aaatcccaat cgataatctt tgtttttttc
      961 gcttctagta aatattttta tagagtttat ttctttcagg caccgcaata cctagaaaag
     1021 aaaattgatg gcgcatatag gcttccatgg accttgcttt tttagagtct tttgctgctt
     1081 ctagctcctc aagtaaatct gctaaactca tctaaaactc ctcatgcccg accaaatggt
     1141 gctgaaaggc atagacagcc gcctgggtac gatcgctaac ttcaagtttg gcaagaatgt
     1201 tggacacgtg ggtcttgacc gtcttgagag agataaagag gtcatctgcg atgcgctgat
     1261 tttcgtagcc cttggcgatg agttggagaa catctcgctc acgcgcagtc aattcttcat
     1321 gaagttccat atgattgcgg tggtattcaa ccttcttgct aacctcttgc tcaatggcca
     1381 gctcgccagc agctacctta ctgacggcat gaagcaattc atctgcacta gaagtcttga
     1441 gcatatagcc tttggcacca gcatctaaga ctggcatgat tttttcattg tccaaataag
     1501 aggtcacaat caaaatcttg gcttcaggcc attctttaag gattgctaag gtcgcgtcaa
     1561 tcccattcat ctcaggcatg acaatatcca tgacaatgac atctggacgc agttccaagg
     1621 ccaagtcaat cccttgagac ccgttggacg cctcacccac aacttctaca tcgtcttgga
     1681 ggtcaaagta gcttttcaaa cccaaacgga ccatttcatg gtcatctact agtaaaattt
     1741 tcatctttac tcctttatca ttccttatct aacaggggaa tacggatatc aactgccagc
     1801 ccttgcttgg gagctgttaa taactgaacc gtccctgcca tatcttcaac ccgctccttg
     1861 atatttcgca gtccataact caagtcatct aagctcccta actggaaacc aatcccattg
     1921 tccaccacct tcagttgcaa ttcaacatct gtctgataga ggtagacatc taggcaagat
     1981 gcctgggcat ggcggagcgt attgctaatc aactcttgca agatacggaa gatatgctcc
     2041 tcgattttct taggcaattt cgtcatattc tgcttgagac taaccctaag atcactcttg
     2101 tcctcaagct cttttaagag aatctgaatc ccttctatca agctcttctg ctccagttca
     2161 actggtcgca aatgcaagag caaaacccgc aaatccttct gggctgtttc taaaatagct
     2221 gtgacactct gcaactgggt ctgcatcttt tctctatcca atttcaaagc ctgctgactg
     2281 atacccgata aaatcatgtg ggccgcaaac aactcctgac tgactgtatc gtgcaaatcc
     2341 cgagcaattc gcttccgttc cttctcgatg atttcctctt cctgagcaag gctatgattt
     2401 tcagcttttt gaagagcttc tgtcaaaagg ttaagtttac ctgataagga cttgaaactg
     2461 gcatccaaat ctggatctgc aacctgaacc acttcttgcc ctgccaataa acgcttgaga
     2521 ttagcctgca tttttcttag agaaagctct tcgatccctc gccaaaacag ggctaagaga
     2581 caggttatgg acatgctgaa aaccaacaat aaaaagacaa atttttctgt tttttcgaca
     2641 tcgtgcaaaa agatagacca gtcaaaatca agtatttcca gcaagctgtg ggagaaaaaa
     2701 aagacaaaca ggaaggaggt gagagcaata atgacatagg cttgtttttt catcctctaa
     2761 ccacctccac atcaccaatc atagtggtca agaaaatctt gacactcttg ttactcttga
     2821 gatagtcttt tgtttcttga tgatagtgtt cattgcggag ggctcgcttg ggctggttga
     2881 aaaaagtcaa atccccatag agacagttaa cgctgagact gacttccaca tctacaggta
     2941 cgatgatttt ggtcgttcct accatctttc tgaggataat gacattgtca tgattggtta
     3001 agatgaccct ctccagatga atagtgtcct tgcccatgaa gcgaaagaga ttgatatcat
     3061 cgaattggca agtctggtag cttgaaaaat gatgaagatt tccaaaccaa cgatttttct
     3121 ccttcttaac cgtcacgacc tcttcaaaaa ccaaattggt ctgctctttt tcctggttca
     3181 tcatcgggta aagaagaaag aggctataga taaccgcaac aaaaatagct agaatcacaa
     3241 aaggattgag cataacgatg aaaaagaaga ggatggttgc cgctactaaa agaagattat
     3301 ttccctcttt accagtgtag tagcgaatca aaagcaaaaa gaggaatagt atcagcagaa
     3361 aacgcgaaaa atgctctgat accatcaaaa tcagagctcc tgtcagaaga caggcttcga
     3421 taaataaaaa gattttaaat tttctcatag gttcatcctc tcccttctat tttatcacaa
     3481 ttcaaaaaag tcacctcagt ctgaggatgg aaaaaaaggc gctggttacg cctttttcat
     3541 ctgatcattt gcttctttta attttccata aagaagatag tctacttttt gtagatctgc
     3601 tatggtggca cagttaaggg aacacataat caagcgtaga tctgctttcc agccttggac
     3661 aatgccaatc acttcttcaa ctgtgtaggt ttcaaccaat tccagaacgg ttcgtgacaa
     3721 tcccacagcc ttagcaccaa aaaccaagca cttaatcata tccagcggat tccgaacccc
     3781 tccactaacc aagagttcga ccttatcttt ccattcttgg gcattgagaa gggcctgcat
     3841 ggtagactga ccccattgat tgaggtaatc acgctggcca ctacgacggt tttcgatata
     3901 ggcaaagctg gtgccaccac gacccgatag gtccactgta cgaacaccga attcataggc
     3961 tctttcgatt gtcttggcat ccattccaaa gcccacttcc ttgaggacaa taggaacggg
     4021 aatttgcttg ctataatctg ctagatgcga ttgccagctt ctaaacttcc tttctccctc
     4081 gggcatgagt aattcctgca tgacattgac atgcacttgc aatagaacag gattcatctc
     4141 ttctacagtc tgaagtccta actcgacagg cttgtccaat ccaatattgg ttccaaggag
     4201 gagattggga tgactagact tgacagaaaa agaatcatcc gttggatttt tgagggctgc
     4261 gctataagaa cccgttacaa ataaaatacc acaggtttcc gccacctgag ccagcttttg
     4321 attgatttct cttcccttat tacttccacc agtcatggca ttgatataaa aaggaaagtc
     4381 ccactttcga ccagcaaact ctgtcgaaag atcgatttca tccagattgt aaagaggcaa
     4441 ggaagaatga atcagctcca cctcatcaaa gctattatag gaacttttct gctcaagggc
     4501 atagaggata tgctcgtcct tacgatttgt cgtcatgtcc tatcctttct tgatataaga
     4561 gctcaatccc cagatcggcc caacgatttt ttaaggtttt ggttgattgc gcatcaaaac
     4621 tcagggcgat gccacagtca ccaccaccag caccactact cttggcaacg gcctgcaaat
     4681 cttgactggc ttctttcaac tgtctaagca aaggcgtgta aatatctgta ctcaagcctt
     4741 ctaaaagctt gctggctact tctacttgct cgataatctt ttctgatttc ccctgttcca
     4801 aggcttcgac caaagaaacc accgtttctt ttgaggaaat taaaaaattt tgattgatat
     4861 tttgcttgat ttgctggacc atgtgactcg atacagccac ttccttggtc catcccacta
     4921 agaaatcaca ttctaaagtt ggtttcactt gtgaaattga aaagccccaa tcacgctcca
     4981 gaactgtcgc caagttttct tcttctaacc aagcagccac cttctggcga tcaaatgact
     5041 ggtagacaac caaatcctct gccacaatac aggcaaggtc tcccatggaa ccattgtctc
     5101 ctcgcttgag caagacagcg ctagtcagct tgaacaagag ctcctgatca acagaaacat
     5161 catacagagc cagtaaagcc ttgacaacca agacaacgac gctgccacta gaacctagac
     5221 caaacttttt cccttctcgt tccattttgc cacagatttc tagagaaaaa ggtcttaaat
     5281 tctgaccacg aacagcgagg aagtctccca tcaaagcaat cgtttcttga atcaagctat
     5341 agtcaggatt gggccttaag tccactgcga aatcaaacat atctgaatag atacggtagc
     5401 tgtcagaaaa agcaatctca gccctcatat agatgggaat atcctttatc aaagctaact
     5461 gccctggctc taaaatagca tattcacctg cccaatagag ttttccgcaa gttttaacag
     5521 caatcatctt gactcaaatc ctttgttttt gacacaatca agcgataacg ctgaccgaaa
     5581 atttctgaca aatgctccaa gtctttctcc tgacagaaga ccttaacatt gggaccagca
     5641 tccatggtaa agtagcaggc ctctcctttc tcacgaagct gacgaacaaa gtccatagcc
     5701 tcataagagg catccgtcag ataagaaaag gctggactag cagtctttgt cgtagcatgc
     5761 atagccaggg catttttctc cgttaattct ccaatcttgg caaaatcatt ttccttgaga
     5821 taaatcagca tatcctgata gtccttctca gactgacgaa cccagtcgtc gaaagtcgtc
     5881 gaggtttcca cacaaagttt catcccgtca cggctagaga ttggtttttt cttgtcctct
     5941 agcaccaaca taatcatagc tagtttcaag tctgtctcta cagggtaaat ttctccacta
     6001 tccttatccc aggctcctag tggtccataa aaactccgag aagaagagcc tgaggcaaat
     6061 ttggcttcct gtgccaactg acttctatcc aatccaagct tgaaataagc attacaagcc
     6121 ttgaccaggg cggacaaacc actagaactt gaggacaggc ccgctgccgt aggcatattg
     6181 ttttgagtat cgatacggac aaagccctca ccagctggac gataacggtc aataatctta
     6241 ctcatcttgg catgctcgac ctcattttgt agctgaccat tgatgtaaaa ttcgtcagct
     6301 gttacattgg ctggtaaagg cgacaaggtc gtctctgtat acatattttc caaagttaga
     6361 gaaatactgc tagtagcagg caccatctct ttttcttttt tctttcccca atatttgata
     6421 atagcaatat ttgcgtagga acgtactgtc acaggctctc tatccatgtc tgaacagctc
     6481 ctttctcttc taatctttct gctagttctt gtgcgtgtgt caaattggtt accaaggcta
     6541 tgatacaacc tcctagccca ccaccgctca tcttggcacc cagagcacca tggctaagag
     6601 tcgtttcaac caaagagtct gcctcaaggc tactgacacc aatttctttt aaatgtaaat
     6661 gtgcttgact gaggatttgt cccagccctt cagcatcttt ttgtgaaatc gcaacttctg
     6721 cctgctgggt taattctccc aaggcatgca aaaacggtag ggcatccttg cccttatttt
     6781 gaaccacttg gatggcttca cgagtatgac cataaacacc cgtatcggca atcaccaaat
     6841 aggcggataa atccatctca agttctgtaa atcctacgtt cttgataaag cgaataggtt
     6901 ggtcactgag acaggtctta gcatccaaac cactaggatt catatgggca atcatttcag
     6961 ctcgattgac caagatttct agtacatcat gaggcagatc agcctgatag tagtcaaata
     7021 ccgcacgaat ggccgctatg ctgatagccg ctgacgaacc catcccccgt ttctcaggga
     7081 tagccgagtc aatctcacaa cgaatgcagg cttctgtgat attcaaatac tccagtgagg
     7141 cataaaccgc catggacaag gtatcctcct cataaaggcg ccaaggactc tctgcaggaa
     7201 ctaccttaca ggtcacctcc acctccaaaa gaggcaggga aatggcagga taaccgtaaa
     7261 cgaccgcatg ttcccctatc aaaattatct tactatgtgc ctgaccgaca ccaacttttt
     7321 ttgtcatttt ttccttttac tagacgaaaa aacgtcttat ttttcataca agtattaatt
     7381 ctttcctatc tattttatta tattttcaca aaaaaagcga ttgtttccat tcacaatcgc
     7441 ttctttcatt attgaaccca ttcgccatta tagttgacag aatagccatc tacagtcgta
     7501 ttcactgcca aggcacctga gctataagcg tagtaccatc tgccattgac ctggaaccaa
     7561 cctgtcgtca tagaacgacg aaagaaactc cataccgtta agtaaagagg aaagtcgtga
     7621 gggagcatgc gccattgaca acctgtttta gtgacgtaca aagtctcatt aacaagtact
     7681 cgtttcggcc atttataggt gcggtgtttg gagaaatagg gttcaatctt agcccattct
     7741 tgatcattta aatcagtatc atatgctttg cgtgtcataa ctctagttta acattttttt
     7801 gtgaatagag gttctaaata atcgaccacg aaaatttctt aagtggaaaa cgccttatga
     7861 agtatgctac gggaaagtta tgcacttaat ttgacaattc aagatgtaaa aatatatact
     7921 a
//
LOCUS       DAVGJL010000097         7572 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.098, whole genome shotgun sequence.
ACCESSION   DAVGJL010000097 DAVGJL010000000
VERSION     DAVGJL010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7572
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.098"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            21..563
                     /locus_tag="VVE42_001646"
     CDS             21..563
                     /locus_tag="VVE42_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEU3073814.1"
                     /translation="MQESNKRLKTKRTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYKDKYDMIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSTDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDTN"
     gene            complement(604..677)
                     /locus_tag="VVE42_001647"
     tRNA            complement(604..677)
                     /locus_tag="VVE42_001647"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(641..643),aa:Asn,seq:gtt)
     gene            complement(683..754)
                     /locus_tag="VVE42_001648"
     tRNA            complement(683..754)
                     /locus_tag="VVE42_001648"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(719..721),aa:Glu,seq:ttc)
     gene            complement(806..1558)
                     /locus_tag="VVE42_001649"
     CDS             complement(806..1558)
                     /locus_tag="VVE42_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU3073815.1"
                     /translation="MKVLILEDVIEHQVRLERILDEISKESNIPISYKTTGKVREFEE
                     YIENDEVNQLYFLDIDIHGIEKKGFEVAQLIRHYNPYAIIVFITSRSEFATLTYKYQV
                     SALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
                     IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRK
                     NLEIVFYEDHRCPISRLKIRKLKDILEKKSQK"
     gene            complement(1555..2880)
                     /locus_tag="VVE42_001650"
     CDS             complement(1555..2880)
                     /locus_tag="VVE42_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU3073816.1"
                     /translation="MDLFGFGTVIVHFLIISHSYHFICKGQINRKELFVFGAYTLLTE
                     IVFDFPLYILYLDGLGIERFLFPLGLYSYFRWMKQYERDRGLFLSLLLSLLYESTHNF
                     LSVTFSSITGDNFVLQYHFPFFFVVTVLTYFVTLKIIYYFHLELAYFDEDYLYPFLKK
                     VFFALLLLHIVSFVSDMVSTIKHLNSFGSILSSIVFISLLLTFFAMNSHKVQMEKEIA
                     LKQKKFEQKHLQNYTDEIVGLYNEIRGFRHDYAGMLVSMQMAIDSGNLQEIDRIYNEV
                     LVKANHKLRSDKYTYFDLNNIEDSALRSLVAQSIVYARNNGVEFTLEVKDTITKLPIE
                     LLDLVRIMSVLLNNAVEGSADSYKKQMEVAVIKMETETVIVIQNSCKMTMTPSGDLFA
                     LGFSTKGRNRGVGLNNVKELLDKYNNIILETEMEGSTFRQIIRFKREFE"
     gene            complement(2901..3026)
                     /gene="comC"
                     /locus_tag="VVE42_001651"
     CDS             complement(2901..3026)
                     /gene="comC"
                     /locus_tag="VVE42_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence-stimulating peptide ComC"
                     /protein_id="HEU3073817.1"
                     /translation="MKNTVKLEQFVALKEKDLQKIKGGEMRLSKFFRDFILQRKK"
     gene            complement(3191..3264)
                     /locus_tag="VVE42_001652"
     tRNA            complement(3191..3264)
                     /locus_tag="VVE42_001652"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3228..3230),aa:Arg,seq:tct)
     gene            complement(3308..3787)
                     /gene="rlmH"
                     /locus_tag="VVE42_001653"
     CDS             complement(3308..3787)
                     /gene="rlmH"
                     /locus_tag="VVE42_001653"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEU3073818.1"
                     /translation="MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTP
                     DKASESENQKILEIEGQRILSKIADRDFVIVLAIEGKTFFSEEFSKQLEETSIKGFST
                     LTFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK"
     gene            3970..5151
                     /locus_tag="VVE42_001654"
     CDS             3970..5151
                     /locus_tag="VVE42_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEU3073819.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLRSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVVVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLE
                     S"
     gene            5209..5967
                     /locus_tag="VVE42_001655"
     CDS             5209..5967
                     /locus_tag="VVE42_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEU3073820.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEARAYESLVKKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLEALLTEKKQKKQQKTNHFIQNEEKQLRKLL
                     GLDVEIKLSKKDSGKIIISFSNQEEYSRIINSLK"
     gene            6180..7541
                     /gene="dnaA"
                     /locus_tag="VVE42_001656"
     CDS             6180..7541
                     /gene="dnaA"
                     /locus_tag="VVE42_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEU3073821.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYDYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVAMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKK
                     KIK"
BASE COUNT         2631 a         1369 c         1201 g         2371 t
ORIGIN      
        1 tttgtttaca aggaggaaat atgcaagaaa gtaacaaacg cttaaaaaca aagcgaacta
       61 ttgaaaatgc tatggtacaa ttactgatgg aacagccatt tgataaaatt tctactgtca
      121 aattagtaga aaaagccgga attagtcgtt ccagcttcta tactcactat aaggataagt
      181 atgatatgat tgagcactat caaagcaagc tattccatac atttgaatat atttttcaaa
      241 aacatgctca tcacaaaaga gacgctattt tagaagtttt tgaatatcta gagtcagaac
      301 cacttctggc tgcccttctt tctgaaaatg ggactaaaga aatccaaaat ttcttacgaa
      361 ataaacttca tatcatgctt agtacagatt tacaaaagcg atttatgcaa ctgaatctaa
      421 ataccaccga attagaatat agtagcatct atctaactca tgcacttttt ggtgtctgcc
      481 aaacttggat tgcacatgga aaaaaagaaa gttctcaaga aataacagac ttccttatga
      541 aaatgcttgg tgatacaaat tgatacaaaa agaggaacac agctgtgttc ccttttatct
      601 atactccgcc agtaggactc gaacctacga catcatgatt aacagtcatg cgctactacc
      661 aactgagcta tggcggataa aatagtccgt acgggattcg aacccgtgtt accgccgtga
      721 aaaggcggtg tcttaacccc ttgaccaacg gaccttctat ctgtagcaga tataaccatt
      781 atatcaattt cttgctaatt gtcaatcact tttgagattt tttctctaaa atatctttta
      841 attttctaat ttttaatctt gaaataggac aacgatggtc ttcatagaaa acaatttcta
      901 agttttttcg atcaatttct ctgatattgc ctatatttac caaaaatgac ttgtgaggag
      961 aataaaatcg ctgagtatgt ttgtcctttt cctgaatatc tgtcatggta ccataaaact
     1021 cttttgcaaa attcttacca ataatgcgca atttatgaga tacccctgtt gtttcaatat
     1081 acaaaatatc atggtaagga atttttaaat catttccctt gtaattgtag tcgaaataat
     1141 ctacaacatc ttcattttca agtaacatac tcttcgtgta gaagatattt tgctcaattc
     1201 tcttcttaaa catctcatca ttgatatcct tatcaacaaa atctagggct gatacctgat
     1261 atttataggt tagagtcgca aactctgatc gactagtgat aaagacgata atagcgtaag
     1321 gattgtaatg acgaatgagc tgagccactt caaatccctt tttctcaatt ccatgaatat
     1381 cgatatctag gaaataaagc tgatttactt catcattttc aatgtattct tcaaattcac
     1441 ggacttttcc cgttgtcttg tatgatattg gaatattcga ttctttcgaa atttcatcca
     1501 atattctctc tagtctcact tgatgttcaa taacatcttc taaaattaaa actttcattc
     1561 aaattccctc ttaaatctaa tgatttgtct aaatgtactg ccttccatct ctgtttctaa
     1621 aataatattg ttgtacttat ctagtagttc tttcacatta tttaatccga ctccgcgatt
     1681 tcttccctta gtggagaatc ctaaggcaaa tagatctcct gaaggagtca tcgtcatttt
     1741 acatgaattc tgaatcacaa taactgtttc agtttccatc ttaataactg ctacttccat
     1801 ctgcttttta tagctatcag ccgatccttc gacagcatta ttcaataaaa cgctcatgat
     1861 acgaaccaaa tccaatagtt caattggaag cttggtaatc gtatctttta cttccagtgt
     1921 aaactctaca ccattatttc gagcatagac aattgactga gcaaccaaac ttcgtaaagc
     1981 tgagtcttct atgttgttca aatcaaagta agtgtactta tctgaacgca atttatgatt
     2041 tgctttgact aaaacttcat tgtaaattct gtcaatttcc tgtaaattac cactgtcaat
     2101 tgccatctgc atgctgacaa gcattccagc ataatcatgt cgaaaaccac ggatttcatt
     2161 atacagacca acaatttcat ctgtgtaatt ctgtaaatgt ttctgttcaa atttcttctg
     2221 cttcaaagca atctctttct ccatttgaac tttatgagaa ttcattgcaa agaaggtcaa
     2281 aaggagagag ataaagacaa tagatgacaa aatacttcca aaactattca aatgtttaat
     2341 cgtacttacc atatctgaaa cgaaagatac aatatgtagc aatagtaaag caaaaaatac
     2401 ttttttcaag aaaggataaa ggtagtcctc gtcaaaatag gctagttcca aatggaaata
     2461 gtaaatgatt tttaatgtaa caaaataggt taacaccgtc acaacgaaaa agaatgggaa
     2521 atgatattgt aaaacaaaat tatctcctgt tatagaggag aaagttacgg acagaaagtt
     2581 atgagtgctc tcatataaaa gagatagtag taaacttagg aatagtcctc tatccctctc
     2641 atactgtttc atccatcgaa aataggaata taatcccaaa ggaaataaaa atctttcaat
     2701 ccctaaccca tctaaatata gaatatataa gggaaaatca aatactattt ctgttagtaa
     2761 tgtataagca ccaaaaacga ataattcttt tctatttatt tgacctttac aaataaaatg
     2821 gtaactgtga ctaataatta aaaaatgaac aataaccgtc ccaaatccaa ataaatccat
     2881 tactctttcc ccttatttca ttactttttt ctttgtaaaa taaaatcacg gaagaatttt
     2941 gacaacctca tctccccacc tttaatcttt tgtaagtctt tttccttcaa agctacaaac
     3001 tgttccaatt taactgtgtt tttcataata aaatctccta aaatgttttt tcttgtaagc
     3061 taacttacaa aaaccattat acaaaatgga atttcgtttt agataaaatt ctctcaactg
     3121 tcattttttt ctcccaaagt gtactttttt aagaaaaaag ccgggaaaat tcccagcttt
     3181 gctattatat tgatcccagc aggattcgaa cctgcgaccg ttcgcttaga aggcgaatgc
     3241 tctatccagc tgagctatga gacctaatac aattattcta ccaaaaattc aattaaaagt
     3301 caattttcta tttatggtag gggaatccct gctgaatcgt aaaagcgcga tagatttgtt
     3361 caacaagaac tagtctcatt aactgatggg gtaaggttag gcgaccaaaa ctgacagaaa
     3421 gattggctct attttttaca gatgatgaca atcctaaact tcccccaata ataaaagtaa
     3481 gagtagaaaa tccttttata gaagtttctt ctaactgctt actaaattct tctgagaaga
     3541 aagttttccc ttcaatggct aacacaataa cgaaatcacg gtcagcaatt tttgataaaa
     3601 ttctctgacc ttctatttct aaaatctttt gattttctga ttcactggcc ttatctggtg
     3661 ttttttcatc tgataactca atcatttcaa acttagcaaa tctagaaatt cgttttgaat
     3721 actctgcgat accatctttt aaatactttt ctttcagttt cccaactgtt acaactttaa
     3781 ttttcatgac tctattctaa catattctct attttttcac atcttattca caaaataaaa
     3841 aatagatttc aattaagaaa atcacagttt caaaagagtt atccacagtt tgtgtaaaac
     3901 ttttgtgttt aagttataat taagctagtc agtttatact ttcagtaatt caaacatatg
     3961 gaggcaaata tgaaacatct aaaaacattt tacaaaaaat ggtttcaatt attagtcgtt
     4021 atcgtcatta gcttttttag tggagccttg ggtagttttt caataactca actaactcaa
     4081 aaaagtagtg taaacaactc taacaacaat agtactatta cacaaactgc ctataagaac
     4141 gaaaattcaa caacacaggc tgttaacaaa gtaaaagatg ctgttgtttc tgttattact
     4201 tattcggcaa acagacaaaa tagcgtattt ggcaatgatg atactgacac agattctcag
     4261 cgaatctcta gtgaaggatc tggagttatt tataaaaaga atgataaaga agcttacatc
     4321 gtcaccaaca atcacgttat aaatggcgcc agcaaagtag atattagatt gtcagatggg
     4381 actaaagtac ctggagaaat tgtcggagct gacactttct ctgatattgc tgtcgtcaaa
     4441 atctcttcag aaaaagtgac aacagtagct gagtttggtg attctagtaa gttaactgta
     4501 ggagaaactg ctattgccat cggtagcccg ttaggttctg aatatgcaaa tactgtcact
     4561 caaggtatcg tatccagtct caatagaaat gtatccttaa gatcggaaga tggacaagct
     4621 atttctacaa aagccatcca aactgatact gctattaacc caggtaactc tggcggccca
     4681 ctgatcaata ttcaagggca ggttatcgga attacctcaa gtaaaattgc tacaaatgga
     4741 ggaacatctg tagaaggtct tggtttcgca attcctgcaa atgatgctat caatattatt
     4801 gaacagttag aaaaaaacgg aaaagtgacg cgtccagctt tgggaatcca gatggttaat
     4861 ttatctaatg tgagtacaag cgacatcaga agactcaata ttccaagtaa tgttacatct
     4921 ggtgtagttg ttcgttcggt acaaagtaat atgcctgcca atggtcacct tgaaaaatac
     4981 gatgtaatta caaaagtaga tgacaaagag attgcttcat caacagactt acaaagtgct
     5041 ctttacaacc attctatcgg agacaccatt aagataacct actatcgtaa cgggaaagaa
     5101 gaaactacct ctatcaaact taacaagagt tcaggtgatt tagaatcttg attgacatct
     5161 atgtaaagaa agctttacat aagagaaaag atgtgttagt gtagaatcat ggaaaaattt
     5221 gaaatgattt ctatcacaga tatacaaaaa aatccctatc aaccccgaaa agaatttgat
     5281 agagaaaaac tagatgaact agcacagtct atcaaagaaa atggggtcat tcaaccgatt
     5341 attgttcgtc aatctcctgt tattggttat gaaatccttg caggagagag acgctatcgg
     5401 gcttcacttt tagctggtct acggtctatc ccagctgttg ttaaacagat ttcagaccaa
     5461 gagatgatgg tccagtccat tattgaaaat ttacagagag aaaatttaaa cccaatagaa
     5521 gaagcacgcg cctatgaatc tctcgtaaag aaaggattca cccatgctga aattgcagat
     5581 aagatgggca agtctcgtcc atatatcagc aactccattc gtttactttc cttgccagaa
     5641 cagattcttt cagaagtaga aaatggcaaa ctatcacaag cccatgcgcg ttccctagtt
     5701 gggttaaata aggaacaaca agactatttc tttcaacgga ttatagaaga agatatttct
     5761 gtaaggaaat tagaagctct tctgacagag aaaaaacaaa agaaacagca aaaaactaat
     5821 catttcatac aaaatgaaga aaaacagtta agaaaactac tcggattaga tgtagaaatt
     5881 aaactatcta aaaaagacag tggaaaaatc attatttctt tttcaaatca agaagaatat
     5941 agtagaatta tcaacagcct gaaataaggc tgttctttta tttttttatc tcacaaggtt
     6001 atccactatg tttttcgata aaaagcttaa taaatcaata atttcttctt ttatccccaa
     6061 cctgtggata aagtttggta acattgtgga ttatttttca cagcttgtgg aaaattcttg
     6121 ctatctatgg taaaatatct ctagtattaa acttttaaat agtaaaggag gagaaaggat
     6181 tgaaagaaaa acaattttgg aatcgtatat tagaatttgc acaagaaaga ctgactcgat
     6241 ccatgtatga tttctatgct attcaagctg aactcatcaa ggtagaggaa aatgttgcca
     6301 ctatatttct acctcgctct gaaatggaaa tggtctggga aaaacaacta aaagatatta
     6361 ttgtagtagc tggttttgaa atttatgacg ctgaaataac tccccactat attttcacca
     6421 aacctcaaga tacgactagc tcacaagttg aagaagctac aaatttaact ctttatgact
     6481 atagtccaaa gttagtatct attccttatt cagatacggg attaaaagaa aagtatacct
     6541 ttgataactt tattcaaggg gatggaaatg tttgggctgt atcagccgct ttagctgtct
     6601 ctgaagattt ggctctgacc tataaccctc tttttatcta tggaggacca ggccttggta
     6661 agactcactt attaaacgct attggaaatg aaattctaaa aaatattcct aatgcgcgtg
     6721 ttaaatatat ccctgccgaa agctttatta atgactttct tgatcaccta agacttgggg
     6781 aaatggaaaa gtttaaaaag acctatcgta gtcttgatct tttgttaatc gatgatatac
     6841 agtcactcag cggaaaaaaa gtcgcaactc aggaagaatt tttcaatacc tttaacgccc
     6901 ttcatgacaa gcaaaaacag attgtcctaa cgagtgatcg tagtccaaaa catctagaag
     6961 ggctcgagga gaggcttgtc acgcgtttta gttggggatt gacacaaact atcacacccc
     7021 ctgactttga aacacgtatt gccattttac aaagtaagac ggaacattta ggctacaatt
     7081 tccaaagtga tactctagaa tacctagctg ggcaatttga ttcaaatgtt cgagatcttg
     7141 agggagccat caacgacatc actttaattg ccagagtaaa aaaaatcaag gatatcacta
     7201 ttgatattgc tgcagaagcc attagagccc gcaaacaaga tgttagccaa atgctcgtca
     7261 tcccaattga taaaatccaa actgaagttg gtaactttta tggtgttagt atcaaagaaa
     7321 tgaagggaag tagacgcctt caaaatattg ttttggcccg tcaagtagcc atgtatttat
     7381 ctagagaact aacagataat agtcttccaa aaattgggaa ggaatttggg ggaaaagatc
     7441 ataccacagt cattcatgcc catgccaaaa taaaatcttt gattgatcaa gacgataatt
     7501 tacgtttaga aattgaatca atcaaaaaga aaatcaaata atttgtggat aacttttagt
     7561 tttttatctt tt
//
LOCUS       DAVGJL010000098         7452 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.099, whole genome shotgun sequence.
ACCESSION   DAVGJL010000098 DAVGJL010000000
VERSION     DAVGJL010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7452
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.099"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            210..557
                     /gene="ssrA"
                     /locus_tag="VVE42_001657"
     tmRNA           210..557
                     /gene="ssrA"
                     /locus_tag="VVE42_001657"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(730..817)
                     /locus_tag="VVE42_001658"
     tRNA            complement(730..817)
                     /locus_tag="VVE42_001658"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(781..783),aa:Ser,seq:gga)
     gene            980..1720
                     /locus_tag="VVE42_001659"
     CDS             980..1720
                     /locus_tag="VVE42_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU3073822.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPHSLQEEETIQEKSVKNLQEKKLLAQKAASLIKEKDVIFIDAGT
                     TTAFLIHELVNKNVTVVTNSIHHAAQLVEKQIPTVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGMNGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
     gene            1717..2628
                     /gene="pfkB"
                     /locus_tag="VVE42_001660"
     CDS             1717..2628
                     /gene="pfkB"
                     /locus_tag="VVE42_001660"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEU3073823.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVEPVQLEELKAILSSLTVEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALLVTSEGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDAVEAFKWGVACG
                     TATTFSDDLATAEFIKETYGKVEVEKR"
     gene            2625..4577
                     /locus_tag="VVE42_001661"
     CDS             2625..4577
                     /locus_tag="VVE42_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU3073824.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIDEMIKNLTEHGYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALAELSQYLMKDGFADKLRQATSAGQVIELFDQASEKTEELVQAP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLALSGDTEVYRAANGAK
                     AATASNEKQSLGGALYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALGVPNENLGNLG
                     SYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YAAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGGLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSNA
                     LLYLVSVLVGAIVSGVVYGYLRKPQA"
     gene            4741..6966
                     /locus_tag="VVE42_001662"
     CDS             4741..6966
                     /locus_tag="VVE42_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEU3073825.1"
                     /translation="MLISLGIAILLIFAAFKLGAAGITLYNLIRLLVGSLAYLAIFGL
                     LIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSVLKGTMAQVVTDLT
                     GFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWSVYDIAEFFS
                     RGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKALLDLPPVDMETGEIL
                     TEEAVQNLPPIPEEKWVEPEIILPQAELKFPEQEDDSDDEDVQVDFSAKEALEYKLPS
                     LQLFAPDKPKDQSKEKKIVRENIKILEATFASFGIKVTVERAEIGPSVTKYEVKPAVG
                     VRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFRELWEQSQTK
                     AENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASILMKARPDQV
                     KFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFAKVGVRNI
                     AGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGI
                     HMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLLGRGDMLFKP
                     IDENHPVRLQGSFISDDDVERIVNFIKAQADADYDESFDPGEVSENEGEFSDGDAGGD
                     PLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEIAGVIGPAEGTKPRKVLQ
                     Q"
     gene            complement(7011..7439)
                     /locus_tag="VVE42_001663"
     CDS             complement(7011..7439)
                     /locus_tag="VVE42_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073826.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTVATIASILGLITASITIMN
                     ALDNNKNIKKEPTTSQTTTIVKEIQKESPKENTSPTKETNTSQEKTQQEETPKSSVKE
                     EKKEDQKTATQDSSTPASSKPATENEKQSNAPTSENKSNQ"
BASE COUNT         2129 a         1402 c         1718 g         2203 t
ORIGIN      
        1 tgaaaagtag accgaataat ataaggattg attttatagt ttttaaactc aaatgaattg
       61 aaataaagag agtacgaaaa ttctcatctg aaagtatttt agaataattc tcttcgtgaa
      121 tttcttcaaa acagatagct tcatcttagg tatgtgattt ctttttgcat ttttgagtta
      181 gataaggtat aatggtttta ttgtcttttg gggtcgttac ggattcgaca ggcattatga
      241 ggcatatttt gcgactcgtg tggcgacgta aacgctcagt taaatataac tgcaaaaaat
      301 aacacttctt acgctctagc tgcctaaaaa ccagcaggcg tgacccgatt tggattgctc
      361 gtgttcaatg acaggtctta ttattagcga gatacgatta agccttgtct agcggtttga
      421 taagagattg atagactcgc agtttctaga cttgagttat gtgtcgaggg gctgttaaaa
      481 taatacataa cctatggttg tagacaaata tgttggcagg tgtttggacg tgggttcgac
      541 tcccaccggc tccattattc ctttgcattc cttggtaaaa cgttgttaaa tcaacgtttt
      601 ttatttttat ctttggtatt cctttgcatt cttttgctaa aaagggagtc acaaacagac
      661 cctattttaa aaaaaggata gaaaaaagga tacaacattt gtcgcatcct aaaaataatc
      721 ttttttcgac ggaagacatg ggattcgaac ccacgcacgc tattacacgc ctaccgcgtt
      781 tccaacacgg cctcttaagc ctcttgagta atcttccaat acttactcaa atagtctacc
      841 ataaaggctc ttatcttgca ataaaaattc tagaaataag aaaaaatgat agattttgaa
      901 agaaaatgat aaaaaatgct tgacttcgaa agaaagtatg atagaatgaa tagtgtaaac
      961 gataacagga ggtgattcag tgttaaaaac agaacgtaaa caactaattt tagaggagtt
     1021 aaatcagcat catgtagttt ctctagaaaa attagttagt ttgctagaaa cgtcagaatc
     1081 aacggttcga agagacttgg atgagttgga agcggaaaac aagcttcgtc gtgtgcatgg
     1141 tggagcagaa ctcccccact ccttacagga agaagaaacc attcaagaaa aatctgtcaa
     1201 aaaccttcaa gaaaagaagt tgctggctca gaaagcagcc tctctcatta aagaaaaaga
     1261 tgtcatcttt atcgatgctg gaacaacaac tgcttttttg attcatgaat tggtcaataa
     1321 gaatgttaca gttgtgacca actccattca ccatgccgct cagttggttg aaaagcagat
     1381 tccaactgtc atggttggag gaaacgtcaa gacggcgaca gatgctagta tcgggggcgt
     1441 tgctcttaac cagattaacc aattgcactt tgaccgtgcc tttatcggaa tgaatggtgt
     1501 tgacgatggc tattatacga ctcctgatat ggaggaggga gctgtgaaaa gagctatttt
     1561 ggagaatgcc aagcagacct acgtcttggt ggattcgtca aaaattggac aaacttgctt
     1621 tgccaaggta gcaccactca aacgcgctat cgttatcact agtcaagggc atgagctctt
     1681 gcaggttatt aaggagaaaa cggaggtaat agaagtatga tttatacagt cacactcaat
     1741 ccatccattg actatatcgt tcgtttggac caagtcaaag ttggtagtgt caatcgtatg
     1801 gacagtgatg ataagtttgc tggtgggaaa ggaatcaatg tcagccgtgt cttgaaacgt
     1861 ttgaatatac caaatacagc gacgggattt atcggtggct ttactggtaa atttatcaca
     1921 gatactctag cagaggaaga aatcgagaca cgttttgtcc aagtggcaga agatactcgt
     1981 atcaatgtta aaatcaaagc agaccaagaa acagaaatca acggaacggg tccaactgtt
     2041 gaaccggttc agctagaaga attgaaagct attttatcta gtctgacagt agaagataca
     2101 gttgtctttg caggttcaag tgctaaaaat ctaggcaatg ttatctataa ggatttgatt
     2161 tccttgacgc gccagactgg tgcgcaagtg gtctgtgact ttgaaggaca gaccttaatt
     2221 gatagtttgg actaccagcc tcttcttgta aaaccaaaca atcatgaact tggagcgatt
     2281 tttggggtta aactcgaaag tttagatgaa attgagaaat acgctcgtga gttactggct
     2341 aagggtgctc aaaatgttat tatctctatg gctggtgatg gcgcccttct tgtcacatct
     2401 gagggagctt acttcgctaa accaatcaaa ggaacagtca aaaattcagt tggagctggt
     2461 gattctatgg ttgctggatt cacaggtgaa tttgtcaaat caaaagacgc agtagaagcc
     2521 ttcaaatggg gagtggcttg cggaacggca actaccttct cagatgactt ggcaacggcg
     2581 gaatttatta aagaaacata tggaaaagtt gaggtagaaa aaagatgaaa attcaagacc
     2641 tattgagaaa agatgtcatg ttgctagatt tgcaggcaac tgaaaaaaca gctgtcatcg
     2701 acgagatgat taaaaatttg acagagcacg gttatgtaac agattttgaa acatttaaag
     2761 aaggaatttt ggcgcgtgaa gctttgactt ctactggttt gggtgatgga atcgcaatgc
     2821 ctcacagcaa aaacgctgct gtcaaagaag cgacagttct atttgctaag tcaaacaagg
     2881 gtgttgacta cgagagcttg gatggacaag caactgacct cttcttcatg attgcagctc
     2941 cagaaggtgc caatgatact cacttggcag ccttggcaga attgtctcaa tacttgatga
     3001 aagacggttt tgcagacaaa cttcgtcaag caacatctgc aggccaagtt atcgaacttt
     3061 ttgaccaagc ttcagaaaaa actgaggaac ttgttcaagc acctgctaat gactctggtg
     3121 actttatcgt agctgttaca gcttgtacaa caggtattgc ccacacttac atggcccaag
     3181 aagcccttca aaaagtagct gctgaaatgg gggttggtat caaggtcgaa accaacggtg
     3241 ctagcggtgt tggaaatcaa ctaactgcag aagatatccg taaggctaaa gctattatca
     3301 ttgcagcaga caaggccgtt gaaatggatc gatttgatgg aaaaccattg atcaatcgtc
     3361 cagttgctga cggtatccgt aagacagaag agctaattaa cttggctctt tcaggagata
     3421 ctgaagtcta ccgtgccgct aatggtgcca aagctgcaac agcctctaac gaaaaacaaa
     3481 gccttggtgg tgccttgtac aaacacttga tgagtggtgt atctcaaatg ttaccattcg
     3541 ttatcggtgg tggtatcatg attgcccttg ccttcttgat tgacggtgct ttgggtgttc
     3601 caaatgaaaa ccttggcaat cttggttctt accatgagtt agcttctatg ttcatgaaaa
     3661 ttggtggagc tgcctttggt ttgatgcttc cagtctttgc gggttatgtt gcctactcta
     3721 ttgctgaaaa accgggtttg gtagcaggtt tcgtggctgg tgctattgcc aaagaaggtt
     3781 ttgcctttgg taaaattcct tatgccgcag gtggtgaagc aacttcaact cttgcaggtg
     3841 tctcatctgg tttcctaggt gcccttgttg gtggatttat cgcaggtgcc ttggttcttg
     3901 ccatcaagaa atacgttaaa gttcctcgtt cactcgaagg tgctaaatca atccttctat
     3961 tgccacttct tggaacaatc ttgacaggat ttgttatgct agctgtgaat atcccaatgg
     4021 ctgcaatcaa cactgctatg aatgacttcc taggcggtct tggaggaggt tcagctgtcc
     4081 ttcttggtat cgtccttggt ggaatgatgg ctgttgacat gggtggacca gttaataaag
     4141 cagcttatgt ctttggtaca ggtacgcttg cagcaactgt ttcttcaggt ggttctgtag
     4201 ccatggcagc agttatggct ggaggaatgg tgccaccact tgcaatcttt gtcgcaactc
     4261 ttcttttcaa agataaattt actaaggaag aacgtaactc tggtttgaca aacatcatca
     4321 tgggcttgtc atttatcact gagggagcga ttccatttgg tgccgctgac ccagctcgtg
     4381 cgattccaag cttcatcctt ggttcagcag tagcaggtgg actcgttggt cttactggta
     4441 tcaaactcat ggcgccacac ggaggaatct tcgttatcgc ccttacttca aatgctctcc
     4501 tttacctcgt ttctgtcttg gtaggagcaa tcgtaagtgg tgtggtttat ggttacctac
     4561 gcaaaccaca agcataaaaa atagaaaaat gaaaagattg gaccgtttgg tgcagtcttt
     4621 ttctcttccc gaaatgcctg tgaaatatgg tataatagaa gaatggcaaa caagaataca
     4681 agtacaacaa gacggagacc gtctaaagca gaactggaaa gaaaagaagc gattcaacga
     4741 atgttgattt cattaggaat tgcgatttta ttgattttcg cagccttcaa attaggggct
     4801 gcaggtataa ccctttataa tttaattcgc ttgctagtgg gtagcctagc ttatctggcg
     4861 atattcggcc tattaatcta tctcttcttt ttcaagtgga tacgaaaaca ggaaggactc
     4921 ttatctggct ttttcaccat atttgctggc ttactcttga tttttgaagc ctacttggtt
     4981 tggaaatatg gtttggacaa gtccgttcta aaagggacca tggctcaggt tgtgacagat
     5041 ctgactggtt ttcgaacgac tagttttgct ggagggggct tgatcggggt cgctctttat
     5101 attccaacag cctttctctt ttcaaatatc ggaacttact ttattggttc tattttgatt
     5161 ttagtgggtt ctctcttagt cagcccttgg tctgtttacg atattgctga atttttcagt
     5221 agaggctttg ccaaatggtg ggaagggcac gagcgtcgaa aagaggaacg ctttgtcaaa
     5281 caagaagaaa aagctcgcca aaaggctgag aaagaggcta gattagaaca agaagagact
     5341 gaaaaagcct tactcgattt gcctcctgtt gatatggaaa cgggtgaaat tctgacagag
     5401 gaagctgttc aaaatcttcc acctattcca gaagaaaagt gggtggaacc agaaatcatc
     5461 ctgcctcaag ctgaacttaa attccctgaa caggaagatg actcagatga cgaagatgtt
     5521 caggtcgatt tttcagccaa agaagccctt gaatacaaac ttccaagctt acaactcttt
     5581 gcaccagata aaccaaaaga tcagtctaaa gagaagaaaa ttgtcagaga aaatatcaaa
     5641 atcttagaag caacctttgc tagctttggt attaaggtaa cagttgaacg ggccgaaatt
     5701 gggccatcag tgaccaagta tgaagtcaag ccggctgttg gtgtaagggt caaccgcatt
     5761 tccaatctat cagatgacct cgctctagcc ttggctgcca aagatgtccg gattgaagca
     5821 ccaatccctg ggaaatccct aatcggaatt gaagtgccca actccgatat tgccactgta
     5881 tctttccgag aactatggga acaatcgcaa acgaaagcag aaaatttctt ggaaattcct
     5941 ttagggaagg ctgttaatgg aaccgcaaga gcttttgacc tttctaaaat gccccacttg
     6001 ctagttgcag gttcaacggg ttcagggaag tcagtagcag ttaacggcat tattgctagc
     6061 attctcatga aggcgagacc agatcaagtt aaatttatga tggtcgatcc caagatggtt
     6121 gagttatctg tttacaatga tattccccac ctcttgattc cagtcgtgac caatccacgt
     6181 aaagccagca aggctctgca aaaggttgtg gatgaaatgg aaaaccgtta tgaactcttt
     6241 gccaaggtgg gagttcggaa tattgcaggt tttaatgcca aggtagaaga gttcaattcc
     6301 cagtctgagt acaagcaaat tccgctacca ttcattgtcg tgattgtgga tgagttggct
     6361 gacctcatga tggtggccag caaggaagtg gaagatgcta tcatccgtct tgggcagaag
     6421 gcgcgtgctg caggtatcca catgattctt gcaactcagc gtccatctgt tgatgtcatc
     6481 tctggtttga ttaaggccaa tgttccatct cgtgtagcat ttgcggtttc atcaggaaca
     6541 gactcccgta cgattttgga tgaaaatgga gcagaaaaac ttcttggtcg aggagacatg
     6601 ctctttaaac cgattgatga aaatcatcca gttcgtctcc aaggctcctt tatctcggat
     6661 gacgatgttg agcgcattgt gaacttcatc aaggctcagg cagatgcaga ctacgatgag
     6721 agttttgatc caggtgaggt ttctgaaaat gaaggagaat tttcggatgg agatgctggt
     6781 ggtgatccgc tttttgaaga agctaagtct ttggttatcg aaacacagaa agccagtgcg
     6841 tctatgattc agcgtcgttt atcagttgga tttaaccgtg cgacccgtct catggaagaa
     6901 ctggagatag caggtgtcat cggtccagct gaaggtacca aacctcgaaa agtgttacaa
     6961 caataaaaaa atagcttctt tccgagtttg gagggaagct attttagtgg ctattgattg
     7021 cttttatttt ctgaagttgg cgcattggac tgtttttcgt tttcagtagc aggtttactt
     7081 gaagcaggag tagaagagtc ctgagttgct gttttctgat cttctttttt ctcttccttg
     7141 acgctagatt ttggtgtttc ctcttgctgt gttttttctt gactagtgtt agtctcttta
     7201 gttggactgg tgttttcctt aggggattcc ttttggattt ctttgacaat ggttgtcgtc
     7261 tggcttgtcg taggttcttt tttaatattt ttgttattat ccaaggcgtt cataatcgtg
     7321 atacttgcgg taatcaagcc aaggatacta gcgattgtag caacggtttt ttgaaagact
     7381 gtaaagcctt ttttgatgtg ttctgttttt ggttttgaag ttctacgata gttagacata
     7441 cattctctcc tt
//
LOCUS       DAVGJL010000099         7372 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.100, whole genome shotgun sequence.
ACCESSION   DAVGJL010000099 DAVGJL010000000
VERSION     DAVGJL010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7372
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.100"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            36..290
                     /locus_tag="VVE42_001664"
     CDS             36..290
                     /locus_tag="VVE42_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001834032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-(glutamine-N5) methyltransferase"
                     /protein_id="HEU3073827.1"
                     /translation="MNVLHSEPHLALFADEDGLAIYCRIAEDAKDYLKDGGKIYLEIG
                     YKQGQSVPELFRKHLPEKRVRTLKDQFGQNRMVVVDDGQD"
     gene            277..876
                     /locus_tag="VVE42_001665"
     CDS             277..876
                     /locus_tag="VVE42_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEU3073828.1"
                     /translation="MDRIRQELENGGAVVLPTETVYGLFAKALDEKAVDHVYQLKRRP
                     RDKALNLNIASFEDILHFSKNQPAYLQKLVETFLPGPLTIILEANDRVPYWVNSDLAT
                     IGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFEQILKDFDQEVLGLEDDAFLT
                     GQDSTIVDLSGDKVKILRQGAIKREDILARLPEISFEEA"
     gene            878..1309
                     /locus_tag="VVE42_001666"
     CDS             878..1309
                     /locus_tag="VVE42_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014947335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU3073829.1"
                     /translation="MLRDLQETDVKAICDINQEALGYTFSPEETASQLARLSQDSHHF
                     LLGYEDAANHVLLGYVHAEVYESLYSKAGFNILALAVSPQAQGQGIGKSLLQGLEQEA
                     KRCGYGFIRLNSANHRLGAHAFYEKVGYTCDKMQKRFIRIC"
     gene            1371..2627
                     /locus_tag="VVE42_001667"
     CDS             1371..2627
                     /locus_tag="VVE42_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEU3073830.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMSLIEPGDTVMGMDLASGGHLTHGAPVSFSGQTYNFVSYSVDPKTELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKLREIADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFK
                     EVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQ
                     NLLDEVNITLNKNSIPYETLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTLKN
                     SENEAVLEEVRSAVKELTDAFPLYED"
     gene            2635..3609
                     /locus_tag="VVE42_001668"
     CDS             2635..3609
                     /locus_tag="VVE42_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEU3073831.1"
                     /translation="MDIYIKKAIIHQFSPDDTELFLADKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLANLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSRKYHLIE
                     KRIKYNGTFLNYFSDNLLAVAPKISPKKSIKELEKTAQRIAESFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEAVPEPVQFDEIDASRQLKKFE
                     NQKLSLSNGIELIVPNNVYQDAESVEFIQNENGTYSILIKNIEDIQSK"
     gene            3609..4223
                     /gene="pmp23"
                     /locus_tag="VVE42_001669"
     CDS             3609..4223
                     /gene="pmp23"
                     /locus_tag="VVE42_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HEU3073832.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     KDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGVDIWTAVQAYNFGPAYIDFIAQNGKENTLALAKQYSRETVAPLLGNRTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVRLNLYIIKCFTLFSTSG"
     gene            4297..5271
                     /locus_tag="VVE42_001670"
     CDS             4297..5271
                     /locus_tag="VVE42_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HEU3073833.1"
                     /translation="MRKKLFLTSAAILWAVTAMNSVHAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNASTDTKELKTMTPDVYSKIMNVANDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGMEHAKITVAAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKSGYDANKLNVALADIKSGLAKAKESK
                     GNLTEEDIRKIVEDTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDL
                     KQSIVSQAGDSFKNINLNFDADKALEDGGNFLSSLWQALVNFFKSFGS"
     gene            5345..6976
                     /gene="rlmD"
                     /locus_tag="VVE42_001671"
     CDS             5345..6976
                     /gene="rlmD"
                     /locus_tag="VVE42_001671"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEU3073834.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMR
                     VLKVNKKIGFGKVEKYLVQSPHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDS
                     LYKIAGIADVEVAETLGMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHDLMPLEDFF
                     IQDPVIDQVVVALRDLLRRFDLKPYDEKEQSGLIRNLVVRRGHYSGQIMVVLVTTRPK
                     VFRVDQLIEQVIKQFPEIVSVMQNINDQNTNAIFGKEWRTLYGQDYITDQMLGNDFQI
                     AGPAFYQVNTEMAEKLYQTAIDFAELKKDDVVIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSKKNAQLNNISNAHYVCDTAENAMKNWLKDGIQPTVILVDPPRKGLTESFI
                     KASAQTGADRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVETVALLSK
                     LDVDKHISVEIELDEMDLTSAESKATYAQIKEYVWNKFELKVSTLYIAQIKKKCGIEL
                     REHYNKSKKDKQIIPQCTPEKEEAIMDALRHFKMI"
     gene            7130..7279
                     /locus_tag="VVE42_001672"
                     /pseudo
     CDS             7130..7279
                     /locus_tag="VVE42_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895229.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
BASE COUNT         2357 a         1409 c         1540 g         2066 t
ORIGIN      
        1 cctatatctc tcgtgaagat gagtcagagg taggcttgaa tgttttgcat tcggagcctc
       61 atctagctct ctttgcagac gaggatggcc tagctattta ctgtagaatt gcggaagatg
      121 caaaagacta tctcaaagat ggtggtaaga tttaccttga aattggatac aagcaaggtc
      181 aaagtgttcc cgaacttttt aggaaacatc ttcctgaaaa aagagttcga acactcaagg
      241 accaatttgg tcaaaatagg atggttgtgg ttgatgatgg acaggattag acaagagttg
      301 gaaaatggtg gagctgtcgt tctacctaca gagacggttt acggtctttt tgctaaggcc
      361 ttagatgaaa aagctgtcga ccatgtttac caactcaaac gtcgtcctag agataaggca
      421 ctcaatctca atatcgcctc tttcgaggac atcttgcact tttcaaagaa tcagccagct
      481 tatctacaaa aacttgtaga gacctttttg ccaggtccct tgaccattat tctcgaagcc
      541 aatgaccgag ttccctattg ggtaaattct gaccttgcaa ctattggatt tcggatgccc
      601 agtcacccta tcacactgga tttaattcga gagacaggtc ccttgattgg gccgtctgcc
      661 aatatctcag gtcaggcaag tggtgtaacc tttgaacaaa ttctgaagga ttttgaccaa
      721 gaggttctgg gtctggaaga cgatgctttt ctaactggac aggattcaac tattgtggat
      781 ttgtctggag acaaggtgaa aatcttacgc caaggcgcaa ttaaacgaga agatattctt
      841 gctcggttgc cagagatttc ttttgaggag gcttgaaatg ctaagagatt tgcaagaaac
      901 agatgtgaaa gcgatatgtg acatcaacca agaggctttg ggttatactt ttagtccaga
      961 ggaaacggct agccaactag ctagactgtc tcaggattcc catcatttcc tacttggcta
     1021 tgaggatgca gctaatcatg tcttacttgg atatgtccac gctgaagttt acgaatcact
     1081 ctattccaaa gcaggattta atatcttagc tttagcagtt tcacctcaag cgcaaggtca
     1141 aggtattggt aaaagtttac tacaagggtt ggaacaagaa gcaaaaagat gtggttatgg
     1201 gtttatccgc ttaaattctg ccaatcatcg tctgggtgct catgcatttt atgaaaaagt
     1261 tggctatact tgtgataaaa tgcagaaacg gtttattcgc atctgttagt ttgattttct
     1321 tattgtaaaa tcaaactaat ggactagtca cacaataaag gagaagacct atgatttttg
     1381 acaaagatga ttttaaagca tatgatgctg atctctggaa tgctattgcc aaagaagaag
     1441 aacgccaaca aaataatatc gagttaattg cttcggaaaa cgtagtttcc aaggctgtta
     1501 tggcagctca agggtctatc ttgacaaata aatatgccga gggttaccca ggacgccgtt
     1561 attatggtgg aactgatgta gtagacgttg tagagactct tgctattgaa cgcgcaaaag
     1621 aaattttcgg tgctaaattt gccaatgttc aaccacattc aggaagccaa gctaactgtg
     1681 cggcttacat gtccttgatt gagccaggtg atacggttat gggaatggat ttggcatcag
     1741 gtggtcattt gactcatggg gctcctgtta gcttctctgg tcaaacctac aactttgttt
     1801 cttatagtgt tgatcctaaa acggaactct tagactttga tgctatcttg aaacaagccc
     1861 aagaagtaaa accaaaactg attgtagctg gtgcttcagc ctattctcaa attatcgatt
     1921 tttcaaaatt acgtgaaatc gcagatgctg tcggtgcgaa gctcatggtg gacatggccc
     1981 atatcgctgg cttggttgcg gctggccttc atccaagccc agttccatac gctcatatca
     2041 caacaacaac gacccacaaa acccttcgtg gacctcgtgg tggtttgatt ttgaccaatg
     2101 acgaagaact tgctaaaaaa atcaattcag ctattttccc aggtattcag ggcggtcctt
     2161 tagagcatgt tgtggcggct aaggcagttt ccttcaaaga agttttggat ccagccttca
     2221 aggaatatgc tgccaatgta attaagaaca gcaaggctat ggcagatgtc ttcttgcaag
     2281 accctgattt ccgtattatt tcaggtggaa ctgaaaacca tctcttcctt gttgatgtga
     2341 ctaaggttgt agaaaacgga aaagttgctc aaaacttgct ggatgaagtc aatattaccc
     2401 taaataaaaa ttcaatccct tacgaaacct tgtcgccatt caagacaagt gggattcgta
     2461 tcggagcagc agctattact gcacgtggat ttggtgaaga agaaagtcgc aaagtggctg
     2521 aactcatcat taaaaccctt aagaattcag aaaatgaggc tgtattagaa gaagtgagaa
     2581 gtgcagtcaa agaattgaca gatgccttcc cattatacga ggactaaaac ttttatggat
     2641 atttatatta agaaagccat tattcaccag ttcagtccgg atgataccga gctgttctta
     2701 gcagataagt ttctcaatat tactccaaaa atcgaagaat acctacgtaa aaaaattgaa
     2761 catgtgtatt cagatgaagc caagactggg attttcgaag aagaaaatcc cttcttcaat
     2821 catattacag acgatttgtt ggagacatca gtaacgctgg ctaatctctg gaaagaggag
     2881 tttagcattt ctgaaaatct caagaccaat gacttgattt ttgttcaatt ttctaaagaa
     2941 ggtgtagaac atttcgcttt cttgcgaatt gccctgcggg agaccttgac ccacctcgga
     3001 ggagaagttg ataatccaat caagctgact cagaataacc tgcctggatt tggaacggga
     3061 gctgatgagg ccttggtggt caatcttcag agtcgcaagt atcacctgat tgaaaaacga
     3121 atcaagtaca acgggacttt tttgaactat ttttcagata atcttcttgc tgtcgctcct
     3181 aagatttctc ctaaaaaatc tatcaaggaa ctggaaaaaa cagcccagag aattgctgaa
     3241 tcttttaaca cagatgattt tcaatttcaa tccaaggtca aatcagctat tttcaacaac
     3301 ctagaagaaa gcaatgaatt gtcacctgag aaattggcta atgacctttt tgacaacaat
     3361 ctgacggctc gtttgagctt tattgaccaa gtcaaagaag ccgtaccaga acctgttcaa
     3421 tttgatgaaa ttgatgccag tcgccaatta aagaaatttg aaaaccaaaa actctcctta
     3481 tcaaatggaa ttgagctcat cgttcccaat aacgtctatc aagacgccga gtctgttgag
     3541 tttatccaaa acgaaaatgg aacctactct atcttaatca aaaatatcga ggatatccaa
     3601 agtaaataat gtttaaacga attcgaagag tgcttgtact agcagtcttc ctttttgctg
     3661 gctataaagc ttaccgcgtt catcaagatg tcaaacaagt catgacctat caacccatgg
     3721 tgcgagaaat attgagtgaa aaagacaccc cagcaaacga agagcttgtg cttgctatga
     3781 tttatactga aacaaaagga aaagaaggcg atgttatgca gtctagtgag tctgcaagtg
     3841 gttccaccaa caccatcaat gataatgcct ctagcattcg gcaaggcatt caaactctga
     3901 caggcaatct ctatctggcg cagaagaagg gtgtagatat ctggacagct gttcaagcct
     3961 ataattttgg acctgcctat atcgatttta tcgcccaaaa tggcaaggaa aataccttgg
     4021 ctctagccaa acagtactct cgtgagactg ttgccccctt gcttggtaat aggactggaa
     4081 agacttatag ttatattcac cccatttcca tttttcacgg tgctgaactc tatgtaaatg
     4141 gaggaaacta ttattattct agacaggtac gacttaacct ttacatcatc aaatgtttca
     4201 ctctcttttc aacatctggc tagaccaggt gtttttgtta taagttttct ttaagataga
     4261 tatattactc taaagaagta aaggaggaaa ttccctatga gaaagaaact ctttctgact
     4321 agcgctgcga tcttgtgggc agtaacagct atgaatagcg tccatgcagc aacagatgtt
     4381 caaaaagtta tcgatgaaac ctatgtccaa cctgaatatg tcctaggttc ttccctatct
     4441 gaagaccaaa aaaatcaaac tcttaaaaaa ctgggctaca atgcctcaac agataccaaa
     4501 gaactcaaga ccatgacacc tgatgtttat tctaaaatca tgaatgtggc caatgactct
     4561 agcttacagt tgtattcatc agccaagatt caaaagctag gtgacaaatc gccacttgag
     4621 gtcaagattg aaacaccaga aaatatcact aaggtgactc aggatatgta ccgaaacgca
     4681 gcagtaacgc tgggtatgga acatgccaaa atcactgtag cagcccctat tccagttaca
     4741 ggagaaagtg ctttagcagg gatttactat tcgctagagg ctaatggagc caaggtgcca
     4801 caagctaata aagatttggc tcaagaagag ctaaaagctt tgtcagatat caatgctgaa
     4861 aacaaggaca aatcaggcta tgatgctaat aaattaaacg ttgccctagc tgatatcaag
     4921 tcaggactcg ccaaagctaa agaaagcaag ggaaatctga cagaagaaga tatccgcaag
     4981 attgttgaag ataccttaaa aaattacaaa cttgatcagg tcataacagg aaaccagatc
     5041 aatatcatca tcaattttgc cttgaatctc tcaaagagtg atatcctcag caatgcagat
     5101 ttcactaaaa ccctaaatga ccttaaacaa agcatcgtat cacaagctgg cgacagtttt
     5161 aaaaatatca accttaactt tgatgcggat aaggcgctag aggacggtgg taacttctta
     5221 agctccctct ggcaagccct tgtcaacttc ttcaagagtt ttggttctta agaaatttca
     5281 tggtataatg gatggtaacc gtaacgttgc cgtctttttt gtcggccaat agaaagagaa
     5341 aagaatgtta aagaaaaatg atattgtaga agttgaaatt gttgacttga cccatgaagg
     5401 ggcaggagtt gccaaggtag atggtttggt cttttttgta gagaatgctt taccgagtga
     5461 aaaaattctc atgcgtgtcc tcaaggtcaa taaaaagatt ggctttggaa aagttgaaaa
     5521 ataccttgtc cagtcaccac accgtaatca agatctagat ttggcttacc tgcgttcagg
     5581 aatcgcggat ttaggacacc tttcttatcc agaacagctc aagtttaaaa ccaagcaagt
     5641 caaggacagt ctctacaaga ttgctggaat tgcagatgta gaagttgctg aaacgcttgg
     5701 tatggaacat ccagtcaagt atcgcaataa ggcgcaggtg cccgttcgtc gagtgaatgg
     5761 tgtcttggaa acaggatttt tccgtaagaa ttcgcacgac ctcatgcccc ttgaagattt
     5821 ctttatccag gatcctgtca ttgaccaagt cgtagtagct cttcgagacc tgctccgtcg
     5881 ttttgattta aaaccttatg acgaaaagga acagtctgga ttgattcgga atcttgtggt
     5941 gcgtcgtggt cactattcag gacaaatcat ggtcgttttg gtgacaactc gtccaaaagt
     6001 ttttcgtgtt gaccaattga ttgaacaagt tatcaagcag ttcccagaga ttgtgtctgt
     6061 catgcaaaat atcaacgacc agaataccaa tgcgattttt ggtaaggagt ggcgcactct
     6121 ttatggtcaa gactatatta cggaccagat gttgggaaat gacttccaaa tcgctggccc
     6181 agccttttac caagtcaata ctgaaatggc ggagaaactc tatcaaacag ccattgactt
     6241 tgcagagtta aaaaaagatg acgtggttat tgatgcttat tctggtattg gaaccattgg
     6301 tttatcagtc gccaagcatg tcaaagaagt ctacggtgtt gaactgattc cagaagcggt
     6361 tgagaatagt aaaaaaaatg ctcagctgaa caatatttca aacgcccact atgtctgtga
     6421 cacagctgaa aatgctatga agaattggct taaagatggg attcaaccaa ccgttatctt
     6481 ggttgatcct ccacgcaagg gcttgacaga aagctttatc aaagcaagcg cccaaacagg
     6541 agccgatcgc atcgcctata tctcctgcaa tgtcgcaacc atggcgcgtg atatcaaact
     6601 ctaccaagaa ttgggatatg aattgaagaa agtccagccg gtggatctat ttcctcaaac
     6661 gcatcacgtc gagacggtag cacttttgtc caaacttgat gtcgataagc acataagtgt
     6721 tgaaattgag ctggatgaga tggatttgac aagtgcggag agcaaagcaa catatgctca
     6781 aatcaaagaa tatgtttgga ataaatttga attaaaagtt tcgacattat atattgcaca
     6841 gataaaaaag aaatgtggaa tagaattacg agaacattac aacaagtcta aaaaggataa
     6901 acaaattatt ccacagtgta cacctgaaaa agaagaagcc atcatggatg ctttgagaca
     6961 cttcaaaatg atttaataga aaagaatgac agtatatgac tttctgcatt tattacattc
     7021 ctacttggta taggaacagc tattattcct ttcttgcaag gtatcaatta gaaaataggc
     7081 tcaatataaa gattgatagg atcattttta tatttaaagg agcgttgaaa tgattgataa
     7141 aggcaacaaa aaattttagg ataaatttgc taagttgtat gcccctttta tgaaaaaaga
     7201 taaagaggtt tatgataaag tttgtgaata tcttagtcct catttgaata aagatatgga
     7261 ggtgcttgaa cttgcttgtt ggtttcgtgt cataacagtt atagaggcaa atagttatgt
     7321 aaatataagg agttcaagac ttctaccaaa gtttaaaact caaaaaataa at
//
LOCUS       DAVGJL010000100         7256 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.101, whole genome shotgun sequence.
ACCESSION   DAVGJL010000100 DAVGJL010000000
VERSION     DAVGJL010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7256
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.101"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            485..710
                     /locus_tag="VVE42_001673"
                     /pseudo
     CDS             485..710
                     /locus_tag="VVE42_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001824185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(1399..2904)
                     /gene="pyk"
                     /locus_tag="VVE42_001674"
     CDS             complement(1399..2904)
                     /gene="pyk"
                     /locus_tag="VVE42_001674"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEU3073835.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
                     VAKDDATREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATSSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAVEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
     gene            complement(2963..3970)
                     /gene="pfkA"
                     /locus_tag="VVE42_001675"
     CDS             complement(2963..3970)
                     /gene="pfkA"
                     /locus_tag="VVE42_001675"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEU3073836.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     S"
     gene            complement(4053..7154)
                     /locus_tag="VVE42_001676"
     CDS             complement(4053..7154)
                     /locus_tag="VVE42_001676"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEU3073837.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLLGLEMTVFVDDQEVNLRFLALSSVGYQQLMKLSTAKMQGEK
                     TWSVLSQYLEDIAVIVPYFDRVESLELGCDYYIGVYPETLASEFHHPILPLYRVNAFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQERFPQALDNLEKLISG
                     ISYDLDTSLKLPRFNPARPAVEELRERAELGLVQKGLTSKEYQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGRSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLA
                     ETEGIHLKIEEIDLEDKETLALFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDPVLEDILAPTYGIMLYQEQVMQVAQRFAGF
                     SLGKADILRRAMGKKDASAMHEMRASFIQGSIEAGHTAEKAEQVFDVLEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQIMLNSANSDYVTDALEAGFEVAPLSINTIPYH
                     DKIANKAIYLGLKSIKGVSNDLALWIIEHRPYSNIEDFIAKLPENYLKLPLLEPLVKV
                     GLFDSFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQESEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVQKIKVIRTKKGENMAFLQADD
                     SKKKLDVTLFSDLYRQVGQEIKEGAFYYVKGKIQSRDGRLQMIAQEIREAVAERFWIQ
                     VKNHESDQEISRILEQFKGPIPVIIRYEEEQKTIVSPHHFVAKSNELEEKLNEIVMKT
                     IYR"
BASE COUNT         2204 a         1607 c         1273 g         2172 t
ORIGIN      
        1 ctacagcgta ctattccaaa ctcaatcaac tataaaacaa tatagactag taatggttgg
       61 actcaataag aattgggtac taagataggt ataagtaaaa acaccattag aaattatgaa
      121 aaaagggtta ggtctactaa gaaaaatact atatttgact tagttaaagt attcagtagc
      181 ttgattgatg ccctctttcc tccagttcaa aaggattctc ctagcgatat ccaatctatc
      241 tacgaccaac gggcaccacc cagacaaggt aaagttctga cctatgccga gaggcaactg
      301 tatgatcaga aaaacgaagt atcgtaaaaa tcatcagact ggacgactac agacagacta
      361 cttactctcg tgttaggtag tttatctctg caggtagccg ctcgatgcag gacgacgatt
      421 tagttatgga gtttccatta tcccagacga ctacgaccca atcgcttatg tcgtcagcaa
      481 gtccatggag ccaaagataa gaaacagcga ctacctattt atcaagaata ccccacagat
      541 tgactataat accatcggca tcttccaagt agacggcact aactatgtca agaaactccg
      601 ccaaggctgc ctcaaaaaaa ctcaatcccg aataccccga catccaccta gacgaaagca
      661 accatattcg taccatcggg aaagctatca ggctatatag ggagaattaa aaaaggggga
      721 gaatggcgat ataaaacaaa cagattttaa aagattcgaa aaactttgaa aaagttcatt
      781 ctacatttca aaaaaagaat aagtgaccta aagtagatta gattttgtta aacaatagtg
      841 atatgtgaat ttagagaaag gcatttaaca tcactttgca aaaggcaaat actaaactag
      901 agaaaccgat aaaccagccc cttagttccc atattttcag gcacacactc cttagtacac
      961 tagctgagaa gaatgctata atggttagag tggaacacaa aaacgctaaa acagtaaata
     1021 acatctacac tcacgtaagt aaaagaatgg agcaagctac tttggaggtt ttaaatacca
     1081 tatcattaaa tcgaaaatat atcagatcga atctagacaa gtagattgcg attactaaaa
     1141 catttgtaga aattcatttg actttctaat caatttgtgc atatcttatt tcatactcaa
     1201 tgaaaatcaa agagcaaact aggaagctag ccgcaagctg tacttgagta cggtaaggcg
     1261 acactgacgt ggtttgaatt tgattttcga agagtatcaa tccattatga aagttttgcc
     1321 ccctttttgc cccctggaca aaaaaagagc ccatcataca ggctagaaag ctggatatga
     1381 taggttttta tatttttctt aacgtactgt gcggatacgc attgtgtttg tgcgaacagc
     1441 ttctcctact ggcacaccag caacgataac gatatcatcg cctgactcaa cgagacctgc
     1501 ttctaccgct ttacgttcag cgatttcgaa catatcgtca gttgaagatg gagcatctgt
     1561 caacattggg ataacacccc agttcaacat caagccacgt tctgtcaatt cgtcaaatgt
     1621 caatgctaag atgtcagcat ttggacggta tttagaaatc aaacgtgcag tatgacctgt
     1681 cttagtaaga gttacaacca atttgatatc cattgagcta gtagcatctt taacagcaga
     1741 agccattact tctgtcttag agttacgctc aaatgaatct gaatcaagac gtccgtattc
     1801 attaagaaga gcttgagcgt tcttgtcgat tgtagccatt gtagttactg actcgagtgg
     1861 gtatttaccg tttgcagact cgcctgacag cattgtagcg tcagttccgt cgataacagc
     1921 gttgaatacg tctgatactt ctgaacgagt tgcacgtggt ttttcagtca ttgtttcaag
     1981 catgtttgtt gcagtgataa caactttacc tgcagcattg actttcttga taatcatttt
     2041 ttgataaact ggaaccattt cgaacggtac ttcgataccc atatcaccac gagcaatcat
     2101 aataccatca gctgcttcga tgatttcgtc taagttatcg ataccttgtt ggttttcgat
     2161 tttagcgaac aattgaacat gtccgtttcc agtttcttca cagattgcac gaacttcgtt
     2221 cacatctttt gcagtacgta cgaatgaaat tgcgatgaag ttgatacctt gttcaagacc
     2281 gaaacggata tcgtcgttat cgcgttcagc aagagctggg aaaggaattt tagtgttagg
     2341 gatgttcaca cctttttgtt tagcgatgat accatcgttt tcaacttcaa cttcaaattc
     2401 acgagttgca tcatctttag caaccacacg aagaccaagt ttaccatcgt caaccaaaac
     2461 ttgacgacca acttcaacat catcatagat atcaagagca ccagcaacgt tcaacgcaat
     2521 cacttcacga gttgatttga ttccttgttt agttgcaaca cgaatttttt caccagtttt
     2581 gtatgaatat tctttagctt caccttcgaa caattctgta cggatttcag gtccttttgt
     2641 atcaagaagg aaaccaactt ttttacctgc aattttttcc gcaagtttaa cagttgccat
     2701 acgctcacct tgttcttggt ggtcgccgtg tgagaagttg aatcggaatg tgttagcacc
     2761 agcttcaatc aatttagcaa tgtttttagc tgaagcttca acatcaagtt tttcacccca
     2821 gtatccatcc tcaccgaatt ttttaccacc acggatttct accgcaggac ccaaagttgc
     2881 aacgatcttt acacgtttgt tcatgatttt tgtgactcct ttatatatat gacctttttg
     2941 gtcttattaa ctaaattgta aattatgaca agctcttatt caagctagat agctcaatat
     3001 cggctttgtg cgggttgtta accacaatct taccttctgc agtaaggcta aacaatgccc
     3061 cttcttctgc agtaccaaga attggatttt caaccatttt ttcgttacga ataccaaccg
     3121 caacaccacc gataccttct ttaagaagtt taacagcatg tgcacccata cgtgacgcca
     3181 aaacacggtc acgcgcagtt ggagaaccac cacgttgaat atgtccaagt tctgttacac
     3241 gaaggtcgct tgtatctcca gcttctttaa gtttttgacc aaattcagcc gctgacatca
     3301 caccttcagc taagacgatg atattgtgtt ttttaccaca ttcataacca gctttgatgc
     3361 ttgctacgat atcttccatc ttgaagcctg cttcagggat gatgatttca tcagcaccag
     3421 ttgcaatacc agcccaaaga gcgatatcac cagcgttacg tcccataact tcgattacaa
     3481 aagtacgacg gtgacttgat gatgtatcac gaatcttatc gatagcgtcc atggcagtag
     3541 taaccgctgt gtcaaaaccg attgtaaagt cagtaccaac gatatcgtta tcgattgtac
     3601 ctggaagacc aatagctggg aagccatgtt cagtcaaacg catagcgccg tggtaagatc
     3661 cgtcaccacc gataacaact acaccttcaa ttccgtgttt tttcaattgc tcaatccctt
     3721 taagttgccc ttcaagttga gcgaactctg gataacgagc tgagtgaaga aaagtaccac
     3781 cacgagaaat gatgtcccct actgaagctg catctagggg atgaatttca ccagcaacca
     3841 taccagcata tccgtcatag ataccaaaca cttccattcc ttctgaaatt gcttgacgaa
     3901 caactgcacg gatggcagcg ttcataccag gtgcgtctcc gccactagtc aaaacagcaa
     3961 tacgtttcat attggtttat gctccttttt cttttaacat tcttcttgat tatatcacat
     4021 ttgattttaa aattcttcta ttttccgtat ttttagcgat aaatcgtttt cataacgatt
     4081 tcattcaatt tctcctctaa ttcattggat ttagctacaa aatgatgggg agaaacgatg
     4141 gttttctgtt cctcttcata ccggatgatg actgggattg ggcctttaaa ttgttctaaa
     4201 atgcgtgaaa tttcttgatc cgattcatga tttttcacct gtatccaaaa gcgttcagca
     4261 actgcttctc ttatttcttg tgcaatcatt tgcagacggc catcacgtga ttgtattttt
     4321 ccttttacat agtagaaggc tccctctttt atttcctgtc cgacctgacg atataagtct
     4381 gaaaagagag tgacatccaa ttttttctta ctatcatctg cctgtaagaa ggccatattt
     4441 tcaccctttt tggtacgaat cacttttatt ttctgaactt caaccaaaat aatagcatag
     4501 ctattttctg acaaatttcc gattggggta atcgggtaaa tagccttact tgcaatagct
     4561 tgtagtggat gtttgctgac acctatccct aaaagctctt gttccatata aaatttttct
     4621 tgttccgtcc aatcttccga ttcctgccaa ctataaatag catctccaaa caaacttccc
     4681 aactctttca caaattcaaa tagattagct aagttattaa atactttttg acgatttttt
     4741 tcaaatgaat cgaaaagacc aacttttacc aaaggttcta gcagaggaag tttcagataa
     4801 ttctcaggta atttagctat aaaatcttca atgttagaat aaggtctatg ttcaataatc
     4861 cagagagcta aatcattact gactccttta atggatttca aacctagata gatggccttg
     4921 ttggcaattt tatcgtgata gggaatcgtg ttgatggaca atggggctac ttcaaaacct
     4981 gcttctaagg catctgttac ataatcactg ttagcagaat tcaacataat ctgataaaat
     5041 atggctggat aatgtgtttt aaaataagcc aactggaagg ccaaggctga gtaggcgtag
     5101 gcgtgtgacc tgttaaaacc atagcctgca aacttctcca gaacatcaaa aacctgctct
     5161 gctttttccg cagtatggcc tgcttctatg gagccttgaa taaaggaagc cctcatctca
     5221 tgcatagcag aagcatcctt tttacccata gctcgacgca aaatatcggc cttcccaaga
     5281 ctaaatccag caaatcgctg ggcaacctgc ataacctgct cctgatagag cattatgccg
     5341 taggttggag ccaaaatatc ctccagtact ggatccagaa cagtcacttc ttcctgccca
     5401 tgctttcttg ccacaaaatt attgatatag tcactagcac ccggtcgatt tagagaagta
     5461 gtcgcgacga catcttcaaa acagactggt tgcacacgct taagcagacg aatggcacct
     5521 ggttgctcaa attgaaagat accttttgta ttaccagagg caaataaagc taacgtttct
     5581 ttgtcttcta aatcgatttc ttcaattttc agatgaatac cttctgtttc agcaagcaac
     5641 tcttgcatct tctggacaaa ggtcaaattt cgtagtccca gaaagtccat cttcaaaagt
     5701 ccgctagcct caactccatg agcatcatac tgagtcagtg gaatttcatc accatacttt
     5761 agaggaatgt agttggttaa atcttggtca ctaattacaa caccagccgc atggacagag
     5821 gtttgccttg gatagccctc tatcttgcaa gcaatctcaa aagctttttg gtattctaac
     5881 ttactattga tttgctgacg aaactggaga ttgccctcat aggccgactt aagattgtca
     5941 cgaaaactga ttttcttagt aattgcagat aattcatact ctggcacacc aaagcgtttc
     6001 aagacatctc gaagagcttg cttggctcca aaggttgaaa aagtaacgat ttgtgccgca
     6061 tgtttactac catatttatt accaacatat ctgataaaat ctggacgata aatatctggg
     6121 atatcaatat caatatcagg catggtatag cgttcacgat taagaaagcg ttcaaaaatc
     6181 agatttttct ctactgggtc aatccccgtg atgtctaagg cataagaaac caaactgcct
     6241 actgcagaac cccttcccat tcccatataa tagccattcg atcgtccaaa acgcaacaaa
     6301 tcccaaacaa ccaagaaata atcatcaaag cccatatcat gaataacaga caattcttgg
     6361 tctagtctat cttgatattc tttactagtc aaccccttct gaacaagccc cagttcagca
     6421 cgctctctca actcctctac tgctggtcta gctggattaa aacgaggcag tttcagacta
     6481 gtatccaagt cgtaagaaat gcctgaaata agcttttcta aattgtccaa agcttgcgga
     6541 aaacgctctt ggaatagttt ctctaaagaa cttgctgata taaagacatc ttgtctcgaa
     6601 cgcaagggaa cttctctgag cggtagattt tctttaatcg ctgttaaaac ttgaagaact
     6661 tctctatccc tgctttcaaa agcgttgacc cgataaagag gtaagatagg atgatgaaat
     6721 tcgcttgcta gtgtttctgg ataaacccct atatagtaat cacagcctag ttctaaagac
     6781 tcaactctat caaaataagg cacaatgacc gcgatatcct ccaggtactg ggacaggact
     6841 gaccaagttt tctccccctg catcttggct gtcgaaagct tcatcaactg ctgatagccc
     6901 acactagata gagctaaaaa gcgcaaattc acttcctgat catctacaaa cactgtcatt
     6961 tcaagcccta gcaaaggatg aatgccgtat ttttttgtaa tctctagaaa gtcgaaagcg
     7021 ccataaagat tgtcaatatc catcatagcc aaatgagtgt agccgtattc tttagctgct
     7081 ctcacatact tttcgatcga aatgacgctt tccataaaac tatagactgt ttttgtatct
     7141 agttgtgcga tcaatttaca cttctcctct atccttctca ctatattata ccattttcac
     7201 ctataaatgg cttctcttga gaaaaatttc atcagcttac taatttattt tctatt
//
LOCUS       DAVGJL010000101         7134 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.102, whole genome shotgun sequence.
ACCESSION   DAVGJL010000101 DAVGJL010000000
VERSION     DAVGJL010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7134
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.102"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            17..730
                     /gene="rsmG"
                     /locus_tag="VVE42_001677"
     CDS             17..730
                     /gene="rsmG"
                     /locus_tag="VVE42_001677"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEU3073838.1"
                     /translation="MKPETFYNLLAEQNLPLSNQQKEQFERYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
     gene            1033..2316
                     /locus_tag="VVE42_001678"
     CDS             1033..2316
                     /locus_tag="VVE42_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEU3073839.1"
                     /translation="MKQESTVDLLLDVDQRPSDGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSVALFASGVGTLIYMIATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLKANWFE
                     IPGFYLPFSTGGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLLGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGL
                     GGAILKLGPVTLSGTALSAMTGIILNLILPYENKD"
     gene            complement(2511..4082)
                     /gene="ffh"
                     /locus_tag="VVE42_001679"
     CDS             complement(2511..4082)
                     /gene="ffh"
                     /locus_tag="VVE42_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEU3073840.1"
                     /translation="MAFESLTERLQNVFKNLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKALAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVRHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTPEERENPD
                     LLNPSRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPDLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(4094..4426)
                     /locus_tag="VVE42_001680"
     CDS             complement(4094..4426)
                     /locus_tag="VVE42_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009660419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEU3073841.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDDFLQEQIEI
                     LTSIDNRE"
     gene            complement(4517..4894)
                     /locus_tag="VVE42_001681"
     CDS             complement(4517..4894)
                     /locus_tag="VVE42_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEU3073842.1"
                     /translation="MKIRMRNTIQFDEQLEVIDQLYDVEVHEKGDYSYLLFYNEEKEK
                     VVIKFHGQELVMSRFSNPKTIMRFLKDSDSLAYIPTPMGMQEFIIQTNRYQVDGQKIE
                     LDYQLQNQEGHPFASYQLEITWG"
     gene            4966..6270
                     /locus_tag="VVE42_001682"
     CDS             4966..6270
                     /locus_tag="VVE42_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU3073843.1"
                     /translation="MNEKVFRDPVHNYIHVNNQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTMTAALLHDLGHGAYS
                     HTFENLFDTDHEAITQEIIQNPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPIENGIAFQRNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPEDKDFFARTSLHLLPFFEKNVTLSD
                     YLALDDGVMNTYFQLWMTSPDKILADLSQRFVNRKVFKSITFSQEDQDQLASMRKLAE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESENPRTQIEILQKNGELAELSSLSPIVQSLAG
                     SRHGDNRFYFPKEMLDQNSIFASITQQFLHLIENDHFTPNKN"
     gene            6283..7089
                     /gene="yidA"
                     /locus_tag="VVE42_001683"
     CDS             6283..7089
                     /gene="yidA"
                     /locus_tag="VVE42_001683"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEU3073844.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDQGDYVVTFNGALVQETATGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGTYTVHESTLVSMPIFYRTPEEMAGKEIVKCMFIDEPE
                     ILDAAIEKIPAEFYEHYSINKSAPFYLELLKKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRAMLEVVGNPVVMENGNPEIKKIAKYITKTNDESGVAHAIRTWVL"
BASE COUNT         2032 a         1651 c         1332 g         2119 t
ORIGIN      
        1 aaagaaggaa ataactatga aaccagaaac attttacaac ttgcttgccg agcagaatct
       61 tccactttcg aaccagcaaa aagaacaatt tgaacgttat tttgagctct tggtcgagtg
      121 gaatgagaag attaatttga cggcgattac ggacaaggaa gaagtttatc tcaaacattt
      181 ttacgattcg attgcaccca ttcttcaagg tttgattccc aatgaaacta tcaaacttct
      241 tgatatcggg gctggggcag gatttcctag tctaccaatg aaaattctct atccggagtt
      301 agatgtgacc attattgatt cactcaataa gcgcatcaac ttcctacaac tcttggctca
      361 agaactggat ttgaacggag ttcatttcta ccacggacgt gccgaagatt ttgcccaaga
      421 caagaacttc cgtgctcaat atgattttgt aacagctcgt gcggttgccc gtatgcaggt
      481 cctatctgaa ttgactattc cctaccttaa ggttggtgga aaactattgg cactcaaggc
      541 cagcaatgcg cctgaggagt tgttagaagc taagaatgcc ctcaatctcc tttttagtaa
      601 agtcgaagac aatctcagct acgccctacc aaatagagat ccgcgttaca tcacagtggt
      661 agaaaagaaa aaagaaacac caaataaata tccacgtaag gctggtatgc caaataaacg
      721 cccgctttaa atttttaagt aaaaaaatat ttacaaaatc aacctcgctc tcttattttt
      781 aggctcggga aaaaatgatt tacaaaatca gcctcgctct tttatttcta ggctcgggaa
      841 aaaatgattt acaaaatcat ttttttctgc tatactatcc taagcaaagg tttttaatgt
      901 catcccgtga ggtgacgaag acgcaaaaat atttgaaact ctttaaagtc tagattttag
      961 agaagtctta ctctgagggc ctattgctgt aaaataatgg gctcttttct gatgcccaaa
     1021 agtgaggttt atatgaaaca agaatcaact gttgatttgt tactagacgt tgaccaacgt
     1081 ccttcggatg gaaaaggaat tctccttagc ttccaacacg ttttcgccat gtttggtgcg
     1141 accatcttgg taccattgat tttgggaatg cctgtatctg ttgccctttt tgcttcaggt
     1201 gttggaacac tcatctacat gattgctact ggttttaaag ttccagttta tctaggttct
     1261 tcatttgcct ttatcacagc tatgtcactg gctatgaaag aaatgggggg tgatgtatct
     1321 gctgcccaaa caggggttat cttgactggt ttggtctatg tccttgttgc taccagcatc
     1381 cgatttgtag gaacaaaatg gattgataaa ctcttgccac caatcattat cggtcctatg
     1441 atcatcgtta tcggtcttgg acttgcaggt tcagctgtta ccaatgcagg tcttgtagca
     1501 gacggaaatt ggaaaaatgc tctggtagcc gttgttactt tcctaattgc tgcctttatc
     1561 aatacaaaag gaaaaggctt cctacgaatc attccattcc tctttgccat tatcggtggt
     1621 taccttttcg cactaactct tggcttggtt gactttacac cagttcttaa agccaactgg
     1681 ttcgaaattc ctggtttcta cttgccattt agcacaggtg gtgcctttaa agagtacaat
     1741 ctttactttg gtccagaagc catcgctatc ttgccaatcg ctatcgtaac aatttctgaa
     1801 catatcggag accatactgt tttgggtcaa atctgtggcc gtcaattctt aaaagaacca
     1861 ggtcttcatc gtactcttct tggtgacggt atcgcaactt ctgtttctgc cttccttggt
     1921 ggaccagcca atacaactta cggagaaaat acaggggtta tcggtatgac tcgtatcgct
     1981 tctgtctcag ttatccgtaa cgctgccttc atcgcgattg ccctcagctt ccttggtaaa
     2041 ttcactgcct tgatttcaac tattccaaac gctgtacttg gtggtatgtc aatccttctc
     2101 tatggggtta tcgccagcaa tggtttgaaa gtcttgatta aagaacgtgt tgatttcgct
     2161 caaatgcgaa acctcatcat cgcaagtgct atgttggttc ttggacttgg aggagctatc
     2221 cttaaacttg gtccagttac actttcaggt actgcccttt cagccatgac aggaatcatc
     2281 ttgaacttga tcttgccata cgaaaataaa gactaagagt ctaaatacac ctaatccact
     2341 cagacagctg agtggatttt tcgcatacca taataaaagt gtcttaacaa aattattaaa
     2401 atcaaaaaac gtataatatc agatattcta aaaccttgat actgtacgtt ttatcataga
     2461 aatttttact ttattttctc atcaaatgag atttgcatca atctcttgtc ttacttgcgt
     2521 ttcttcttcg ctttcttcat tttgttagcc atacgtttca tggactgttt catggcaaat
     2581 tcaccaattt tacctttcaa accgccacca aacatctggc tcatatctgg cattcctgct
     2641 cctccgagag ctgataagtc aggcataccg ccttgtccca tcattccttc aagggcagac
     2701 atatccattc ctcccatatt tggcatattt ttaggaaggt tatttggatt aatccccatt
     2761 tgcttcatca ttttattcat atccccagac ataacaccct gcatgagctg tttagcctgg
     2821 ttaaagtcct tgatgaattt attgacttcg acgaatgtat ttccagaacc agcagcaata
     2881 cgacggcgac ggcttggatt taacaaatct gggttttcac gctcttcagg tgtcatcgaa
     2941 gacacaatgg cacgtttacg agcaatctgg cgttcatcca ccttcatgtt ttgaagggct
     3001 ggattgttgg ccatacctgg aatcatcttg agcaagtctt ccatcggccc catattttgc
     3061 acctgatcta attgatcgat gaaatcatta aaatcaaagg tgttttcgcg catcttctca
     3121 gccatttcaa gggctttttg ttcatcgtat tcctgagaag ctttctcaat caaagtgagc
     3181 atatccccca taccaaggat acggctagac atgcggtctg ggtggaaggt ttcaatgtcc
     3241 gtaatctttt cacctgtacc agtgaacttg attggttttc cagtaatgtg acgaacagac
     3301 agagcagcac caccacgagt atcgccatca atcttggtaa ggatgacccc agtcacttcc
     3361 aactgagcat taaactcacg cgcaacattg gctgcttcct gaccaatcat agcatcaacg
     3421 acaagcaaga tttcatttgg ttgagccaat gctttcacat cacgaagctc attcatgagg
     3481 agctcatcaa tctgcaaacg acccgcagta tcaatcaaga catagtcgtt atgattagtt
     3541 tgggcttgct ccaaaccttg acgtacaatc tcaacagctg gtacttctgt tccaagtgca
     3601 aagacaggca catcaatctg ttgtcccaag gtcttaagct ggtcaatggc agctggacga
     3661 taaatatccg ccgcaatcat caaaggacga gcattttctt ctttcttgag tttgttggcc
     3721 aatttaccag caaaggttgt tttaccagcc ccttgtaaac caaccatcat gatgatggtt
     3781 ggaatcttag gtgacttgat aatttctgcc gtatcagaac ctaaaacggc tgtcagttcc
     3841 tcatcaacga ttttaataat ctgttgcgca ggattaagcg tatcaatgac ctcatgcccg
     3901 actgcacgct cacgaacttt cttgataaag tcctttacaa caggcaaggc aacgtcggcc
     3961 tcgagcaagg ccaagcgaat ttctttggtt gcctcttgga catcagattc agagattttt
     4021 ccttttttac gtagattttt aaagacgttc tgcaaacgtt ctgttaaact ttcaaatgcc
     4081 atttttcttc ctcttattct ctattatcaa tgcttgttaa aatttctatc tgctcctgca
     4141 gaaagtcatc cttgggatag cgctccaaaa tctgatcaaa aatctgactg cggacaatat
     4201 agtccgagta catgtgcaat ttcatctcat aatcttccag aatcttttct gttcgcttga
     4261 tattgtcata gacagcctga cgactgacac cgaactcctc ggcaatttca gcaaggctgt
     4321 aatcatcagc gtagtagagc tcgatataat tcatttgctt atctgtcaaa agcgccgcat
     4381 aaaattcaaa gagcgcattc atacgattgg ttttttcgat ttccataact tttattatac
     4441 caaaaattag cctaatctac cacactagga agccgatcca agaagataga tagctaaatt
     4501 tgaaaaagac atgagcctag ccccaagtaa tttccaattg atagctggca aagggatgtc
     4561 cctcttgatt ttgtagttga taatctagtt caatcttttg cccatccact tgataacgat
     4621 tcgtttggat aataaactcc tgcataccca taggtgtggg aatataggct aaactatcac
     4681 tatcctttag aaagcgcata atggtcttgg gattggaaaa tcgactcatc acaagttctt
     4741 gaccatgaaa tttaatcact actttttcct tttcctcatt atagaaaagc aggtagctat
     4801 aatctccttt ttcatgcact tccacatcat aaagctggtc aatcacttcc aactgctcat
     4861 caaactgaat tgtatttcgc atccgaatct tcacatcagg ccctctttct tgtctcttgt
     4921 cctactattt taccaaaaag agcaggattt tgctataatg gtcatatgaa cgaaaaagta
     4981 ttccgtgacc ctgttcacaa ctacatccat gtcaataatc aaatcatcta tgacttgatt
     5041 aatacaaaag aatttcagcg tctgcgccgg atcaaacaac tgggaacttc cagttatacc
     5101 ttccacggtg gagaacacag tcgcttctct cactgtctag gagtctatga aattgcacga
     5161 cgcatcacag agattttcga agaaaaatat cctgaggaat ggaatcctgc cgagtctctc
     5221 ttgaccatga ccgctgctct cctacacgac cttgggcatg gtgcctactc ccatactttt
     5281 gaaaatctct ttgatacaga ccatgaagcc attactcagg agattattca aaatcctgag
     5341 acagagattc accaagtcct gctacaagtg gcacctgatt tcccagaaaa ggtggccagt
     5401 gtcattgacc atacctatcc taataagcag gtcgtgcagc tcatttctag tcagattgac
     5461 gcagatcgca tggactatct cttgcgcgac tcctatttta caggagcatc ctatggggaa
     5521 tttgacctga ctcgaatcct ccgagtcatt cgtcctatcg aaaatggtat cgcctttcag
     5581 cgcaatggca tgcacgccat cgaagactac gtcctcagtc gctaccagat gtacatgcag
     5641 gtttatttcc accccgcaac acgcgccatg gaagttctcc tacagaatct tctcaaacgc
     5701 gccaaggaac tctatcctga ggacaaggat ttctttgccc gaacttctct acacctcctg
     5761 cctttcttcg aaaaaaatgt gaccttgtct gactatctgg ctctggatga tggcgtgatg
     5821 aatacctact tccagctttg gatgaccagc cctgacaaga ttcttgcaga tttatctcaa
     5881 cgctttgtca accgcaaggt ctttaaatcc attacctttt cacaagagga ccaagaccaa
     5941 ctcgccagca tgagaaaatt agctgaggat atcggctttg atcccgatta ctacactgcc
     6001 attcataaga actttgacct cccttatgat atctatcgtc ccgaatctga aaacccacgg
     6061 acacagattg agattttaca aaaaaatgga gaactggccg aactctctag cctgtctcct
     6121 atcgtccaat cccttgctgg aagtcgccac ggagataatc gcttttattt tccaaaagaa
     6181 atgttggacc aaaacagcat ctttgcaagc attacccagc aatttttaca cttgattgag
     6241 aacgatcatt ttaccccaaa taaaaactag aagaggaatt ttatgagtat taaactaatc
     6301 gctgtcgata ttgacggaac cctagtcaac agtcaaaagg agattactcc tgaagtcttt
     6361 tctgccatcc aagatgccaa agaagctggt gtcaaagtcg tgattgcaac tggccgcccc
     6421 atcgcaggtg ttgccaaact tctggacgac ttgcagttga gagaccaagg tgactatgtg
     6481 gtgaccttca atggtgccct tgtccaagaa actgctactg gccatgagat tatcagcgaa
     6541 tccttgacct atgaggatta tctggatatg gaattcctca gtcgcaagct cggtgtccac
     6601 atgcatgcca ttaccaagga cggtatctat actgcaaatc gcaatatcgg aacatacact
     6661 gtacacgaat caaccctcgt cagcatgcct atcttctacc gtacccctga agaaatggct
     6721 ggcaaagaaa ttgttaaatg tatgtttatc gatgaaccag aaattctcga tgctgcgatt
     6781 gaaaaaatac cagccgaatt ttacgagcac tactctatca acaaatctgc tcctttctac
     6841 ctcgaactcc ttaaaaagaa tgtagacaag ggttcagcta ttactcactt agctgaaaaa
     6901 ctaggattaa ccaaagatga aaccatggct atcggagacg aagaaaatga ccgtgccatg
     6961 ctggaagtcg ttggcaaccc tgtagtcatg gaaaacggaa atccagaaat caaaaaaatc
     7021 gccaaataca tcaccaaaac aaatgacgaa tccggcgttg cccatgctat tcgtacatgg
     7081 gtactgtaaa agtatcattt ttcaataaga attgattagc aataaaatcc aatg
//
LOCUS       DAVGJL010000102         6964 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.103, whole genome shotgun sequence.
ACCESSION   DAVGJL010000102 DAVGJL010000000
VERSION     DAVGJL010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6964
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.103"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            87..3437
                     /locus_tag="VVE42_001684"
     CDS             87..3437
                     /locus_tag="VVE42_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEU3073845.1"
                     /translation="MGNFSFLLKNDEYESFSKPCIEAENMIATSTVATAFMARRALEQ
                     AVHWIYSHDSYLEAPYRATLSSLVWDDDFRDIVDSELHKQIVLLIRWGNHAAHGGEIK
                     EREAILALHHLYQFVNFIDYCYSNEFVERYFDEKCLPLSANIKYRETPQSMIKLQDSL
                     PELPDFHEQMAAQSVEVQETYTEKRETAAQRQDVPFHIDQLSEAETRKLFIDIDLRLA
                     GWIFEENCRVEIAVDGLKHGSGIGYCDYVLYGKNGKILAIVEAKKASVNPEVGEVQVK
                     EYAEALEKHIGYQPICFITNGLKHYILDGPNRRQIAGFYSQEELQLVMDRRHLQKPLE
                     DISSKIRDDISGRHYQKHAIASVCEAFSNHRRQALLVMATGAGKTRTAVSLVDILSRH
                     NWVKNVLFLADRTSLVKQAYDSFRKLLPDLSVCNFLEDKEGAQSSRMVFSTYPTMIGA
                     ISGQEEVNQRPFTVGHFDLIIIDESHRSIYQKYKSIFDYFDARIVGLTATPRQDLDKN
                     TYGFFNLENGVPTYAYDLEEAVKDGYLVAYHSIETKLKLPTDGLHYDDLSEEEKEHFD
                     SKFEDDSCEKDIDGSVFNSFIFNKSTVEIVLNELMTRGIQTASGDEIGKTIIFAKNHD
                     HAEYIRGIFNNRYPEKGSDYAQVIDYSIKHYQTLIDDFKIKEKYPQIAISVDMLDTGI
                     DVPEVVNLVFFKKVRSKTKFWQMIGRGTRLCKDLFGPEQDKENFLVFDYGDNFDYFRA
                     DPRDGEGRHIVSLTQRLFNIKVDLIRELQGLQYQEDQFARAYRQQLVSELQGRIESLN
                     ELDFRVRMVLDTVYSYRKLESWQNLTAVTSETIQKNLSPLLFDEDKEDEMARRFDLWL
                     LHIQLGQLTAKSSTVHISQVMKTARALSAIGNIPQVFEQAEIIRKVQEPEFWKEVNLS
                     DLEKIRLAIRDLLQFLDKTDRKPYYVNFEDRILSTVHETTAFLQVNDLRSYNEKVEHY
                     LKTHLDEESISKLYHNKKLTSDDMLALEKLLWEKLGSKADYQSHYENKAIPRLVREII
                     GLDRESANRIFSKFLSDENLNARQISFVKLIVDYIVENGFLETKVLTQEPFKSYGSVQ
                     LLFQHQLPVLRNIVQIIELINNRAGEAA"
     gene            3681..3782
                     /locus_tag="VVE42_001685"
                     /pseudo
     CDS             3681..3782
                     /locus_tag="VVE42_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(4023..4493)
                     /locus_tag="VVE42_001686"
     CDS             complement(4023..4493)
                     /locus_tag="VVE42_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU3073846.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQAELQALLAKNDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(4510..6783)
                     /locus_tag="VVE42_001687"
     CDS             complement(4510..6783)
                     /locus_tag="VVE42_001687"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEU3073847.1"
                     /translation="MRFNQYSYINFPKENILSELKKCGFDLQNTANHKDSLETFLRRF
                     FFTYQDTNYPLSILAADKKTDLLTFFQSENELTADIFYTVAFQLLGFSYLVDFKDSDV
                     FRKETGFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLAT
                     FSSQDVIREVVYVESRVDTDQKGLPDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKA
                     SDKALYKMEGELEVSLPHKIELEKPQLNLVQPQGKAELVAEAEEKLTHINSSYTLNDY
                     FLPRGFATLYVSGVGTKDSTGFMTNGDYQQIEAYKNVIDWLNDRCRAFTDHTRQRQVK
                     ADWSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGG
                     YPGEDFDSLAELTYSRNLLAGDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHDRNYL
                     LNAHKVKAEVVFTHGSQDWNVKPLHVYQMFHALPTHIHKHLFFHNGAHVYMNNWQSID
                     FRESINALLTKKLLGQETDFQLPTVIWQDNTAPQTWLSLDNFGGQENCKTFSLGQEEQ
                     AIQNQYPDKDFERYSKTYQTFNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSS
                     TNKGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKG
                     YLNLQNRNDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNT
                     DYHLTVDLAQSMLTLPC"
BASE COUNT         2142 a         1161 c         1513 g         2148 t
ORIGIN      
        1 gcttgtgaga tagctataaa agtgtggaga aatagcttga aatttagtat aatatagcta
       61 aactatttgt ttaaagtgag aaaaaaatgg gaaattttag ctttctttta aaaaatgacg
      121 aatatgaatc tttttcaaaa ccttgcattg aagctgaaaa tatgattgct acatcaactg
      181 tggctactgc ctttatggcg cgtcgtgctt tagagcaggc tgtccattgg atatatagtc
      241 acgattcata tttagaagct ccctatcgtg ctactctatc ttctttagta tgggatgatg
      301 attttaggga tatcgtagat tctgaactcc acaagcagat agttctgttg attcggtggg
      361 gaaaccatgc tgctcatggt ggtgaaatta aggaacgaga agcgatttta gctttgcatc
      421 atttgtatca gtttgttaat tttatcgatt attgttacag caatgagttt gtggagcgtt
      481 attttgatga gaagtgctta ccactttcag caaacatcaa ataccgagaa actccacaat
      541 ctatgataaa gttacaagac agtttaccag aactgcctga ttttcatgaa cagatggctg
      601 ctcagtccgt agaagttcaa gagacttata ctgaaaaacg tgagactgca gcgcaacggc
      661 aagatgtgcc tttccatatt gatcaattgt ctgaggcaga gacaagaaag ctctttattg
      721 atatcgatct ccgtttagca ggatggatat ttgaagaaaa ctgtcgtgtt gagatagccg
      781 ttgatggtct caagcacggt tcaggaattg gttactgtga ctatgtactt tatggtaaaa
      841 atgggaaaat tttagcgatt gtagaggcta aaaaagcctc tgtcaatcca gaagtagggg
      901 aagtacaggt caaagaatat gctgaagctc ttgagaaaca tatcggctat cagccaattt
      961 gctttattac aaatgggttg aagcactata tacttgatgg tccgaaccgc cgccagattg
     1021 caggctttta ctctcaagaa gaattgcaat tagtgatgga tagacgtcat cttcaaaaac
     1081 cgcttgagga tatttctagt aaaattaggg acgatatttc cgggcgtcac taccaaaaac
     1141 atgccattgc aagcgtttgt gaagctttct ctaatcatcg tagacaggca cttctggtta
     1201 tggcaactgg ggcggggaaa actcgtacag cagtttctct agttgatatc ttatcacgtc
     1261 ataactgggt aaaaaacgtt ctcttcttag ccgatagaac ttccttggtt aagcaagcct
     1321 atgattcgtt tagaaaatta ctcccagatc tttccgtttg taacttctta gaagataaag
     1381 aaggagctca atcaagtcgt atggtctttt caacttatcc gaccatgatt ggagcgatta
     1441 gtggtcaaga agaagtaaat caacgccctt ttactgttgg gcattttgac cttatcataa
     1501 ttgacgaatc tcaccgttct atttatcaga aatacaagtc catttttgat tattttgatg
     1561 caagaattgt aggcttaaca gctactccgc gtcaagattt agataaaaac acctatggat
     1621 tctttaattt ggagaatggg gttccaacat atgcatatga tttggaagag gctgttaaag
     1681 acggatattt agtagcctat cattctatcg aaaccaaact gaaactacct acggatggtc
     1741 tacattatga tgatttgtcc gaagaagaaa aggaacattt tgatagcaaa tttgaagacg
     1801 atagctgtga aaaagatatt gatgggagtg tatttaattc ctttattttc aataaaagta
     1861 cagtagaaat tgttttaaat gaactcatga caagaggaat tcagacagcc tcgggtgatg
     1921 aaattggtaa aactattatt tttgctaaaa atcatgatca tgcggaatat atcagaggta
     1981 tttttaacaa ccgctatcct gaaaaaggga gcgactatgc tcaggtgatt gattatagta
     2041 ttaagcatta tcagaccttg attgatgatt ttaaaattaa ggagaagtat cctcaaattg
     2101 cgatttctgt cgatatgtta gatacaggta ttgatgtacc agaggttgtt aatttagtct
     2161 tcttcaagaa agtacgctct aaaactaagt tttggcagat gattggtcga ggaacccgtc
     2221 tatgtaaaga tttatttgga cctgagcagg ataaggaaaa cttcttggta tttgattatg
     2281 gggataattt tgattatttt cgtgcagatc caagagatgg agagggtcgt cacattgttt
     2341 cgttgactca gcgtttattt aatatcaaag tggacttgat tcgagaactt cagggactcc
     2401 aataccaaga agatcagttt gcgagagcat accgtcagca gcttgtctcg gaacttcaag
     2461 gtcgtataga gagcttaaat gagttggact tcagggttcg tatggtttta gatacagttt
     2521 atagctatag gaaattggaa agttggcaga atctaactgc tgttacaagt gaaaccattc
     2581 aaaaaaatct ctctccgctt ttatttgatg aagataaaga agatgagatg gcgaggagat
     2641 ttgatttgtg gttgcttcat attcagttgg ggcaactgac agctaaatct tccactgttc
     2701 atatttccca agtgatgaag acggctagag ctctttctgc tattggcaat atcccgcagg
     2761 tttttgagca ggctgaaatt atcaggaaag tacaggagcc tgaattttgg aaagaagtta
     2821 acttgtctga tttggaaaaa attcgtcttg ctattcgaga tttattacag tttttggata
     2881 aaacagaccg taaaccctac tatgttaact ttgaagatcg tatactctcc actgttcacg
     2941 agaccacagc atttttgcag gtcaacgatc ttcggtctta caatgaaaaa gttgagcatt
     3001 atttgaaaac tcatctggat gaggagtcca tttctaagct ataccataat aaaaagttga
     3061 catctgatga tatgcttgca cttgaaaaat tgctctggga aaaattaggt agtaaagcag
     3121 actaccaaag tcattatgaa aataaggcaa ttccgagatt ggttcgtgag attattggct
     3181 tagatagaga gtctgccaat cgtatttttt ctaaattttt gtcggatgag aatcttaatg
     3241 ccaggcagat ttcatttgta aaattgattg tagactacat tgtagaaaat ggttttttag
     3301 agacgaaagt gttaacgcaa gagccgttta aatcttatgg ttctgttcaa ctactcttcc
     3361 aacaccaact accagtactt cgtaatattg ttcaaatcat tgaacttatc aataatcgag
     3421 ctggagaagc ggcttaaatt ctaaagtgat tgccatgctg agactcattt aaaattaaaa
     3481 agagtagaaa tttatgctat atatgagaag ttttattagg aagaatgtca tcgttttcct
     3541 agaatacagt atcagttgtt aagtggttga taaatttcaa agtagatact tgtaccacga
     3601 tgtttgttga tcgagttatt aacaaaagag ctactttgat tttaaagaaa tagaaaacaa
     3661 aaagccgagc aagaattcaa ttgcaggaga aaatgaaata atactcaatg aaaatcaaag
     3721 agcaaactag gaaactagct gcaggctgct caaaacactg ttttgaggtt gtagataaga
     3781 ctgacgaagt cagctcaaaa catggttttg aggttgtaga tgaaactgac gaagtcagta
     3841 accataccta cggcaaggcg acgttgacgc ggattgaaga gattttcgaa gagtataaat
     3901 cttcctagga taaagcaaaa cgcatagtat caagggtttt cagcacttga tactatgcgt
     3961 tttctgatgt taaagacttt ctaccagttt ttttaagcat aattgttagt tgtagtcatt
     4021 tattattctt caaagaaaaa tggtggggcg aattttttca gttcttcaaa gcacttttga
     4081 gcagtatctg catcttcaca gatgataaga cagacatcat taccacaaag ggtagcgata
     4141 gcgtcaggga agctcaaagt atcaatgata gaaccaaagg attgagccag tccaggaagg
     4201 gtttttagta ggacttggtg ttgaactggg cgcatccaga caagggcgtc ttccatgtag
     4261 agttcgagac gtttttccca ttttgagatg gaaccattgt taagaacata ataagcgcta
     4321 tcttcttcgc ggacttttga taggttcata tttttgatgt cgcgtgagag ggttgcctgg
     4381 gttacttgaa tgtcgttctt agcaagaagg gcttgcaact cagcctgtgt atgaatcttg
     4441 ttttttgtga taagagcgcg tataagttgg tggcggtgtt ctgatttatt cataataatg
     4501 taactccttt tagcaaggta aggtaagcat ggactgagcg aggtcgacag tcaagtggta
     4561 gtctgtattg tcacggatgg tgatttcaaa gtcagtagta tagaggacta aacggagagt
     4621 gtctccttct tttagcttgt aaatagttgg ctgcagttca aattgaacgt ccatccattc
     4681 atctgcagta atatcctcta ctaacagtaa atcatttcta ttttgtaaat taaggtaacc
     4741 ttttgtcacg actcgttgtg cctctggtct aaatggcaat tcacagagat tttccaacat
     4801 gtgatagcga ccgttgtcaa tggttctagc acttaaaata gctggataag gttgtaggta
     4861 tttcttttgc ccaaattcta gcagttgggc agataagagc cccttgtttg tactggattt
     4921 gatacgaaga ttgagctgag cgcgaccgtt taggtggaga tctttagtca caggaaggtt
     4981 aatagtaatc tgattagctt tcccttgata gagctctgta ttgaaggttt ggtatgtctt
     5041 actatagcgc tcaaaatcct tatctgggta ctggttttga atagcttgct cttcttgacc
     5101 aagtgagaag gttttacagt tttcttgccc accgaagtta tcaagtgata accaagtctg
     5161 tggagctgta ttgtcctgcc agataacagt aggaagttga aagtctgttt cctgtcctag
     5221 taatttcttg gttaataagg catttatgga ctcacggaag tcaattgatt gccaattgtt
     5281 catgtaaaca tgggcaccat tatggaaaaa gagatgcttg tgtatatgag taggaagagc
     5341 atggaacatc tggtaaacat gaagtggttt gacattccaa tcctgagaac catgagtaaa
     5401 gacaacctct gcctttactt tatgggcatt gagcagataa ttgcggtcat gccaaaactg
     5461 attgtagtcc ccagtttttc ggtctagctg agctttcact ttttctaagt cagcttggtg
     5521 agcttcattg ccacggatat agtcgccagc taagagatta cgagaatagg ttaactcagc
     5581 aagggagtca aagtcctcac ctggataacc acctgggcta gtcaccagac cgttttcacg
     5641 gtagtagttg taccatgatg aaattcctgc ctcggcaatg ataacttcta aaccatcgac
     5701 tcctgtagtc gcaagaccat tggacatggt acctagatag gaaagtcccg ttgtagcaac
     5761 ttttccgttt gaccaatcag ccttgacttg acgctggcgc gtgtgatcag taaaggcacg
     5821 gcaacggtcg ttaagccaat cgatgacatt tttataagcc tcgatttgct ggtagtctcc
     5881 attagtcatg aaacctgtcg agtctttggt accaacacct gagacataga gagtggcaaa
     5941 gcctctcgga aggaagtagt cgtttagtgt atagctagag ttgatgtgag ttagcttttc
     6001 ctcagcctct gctacaagct cagctttacc ttggggttgg acgagattta gttgaggttt
     6061 ctctagctca atcttgtgag gaagagaaac ctcaagctcg ccctccatct tgtagagagc
     6121 cttgtcacta gccttatcat tggttccctg atgataaggg ctggctgtca tgatggcagg
     6181 gatttttcca tcaaaacgag ggcgaataat gctaaccttt actaggtctg gtagcccttt
     6241 ttggtcagta tcgacacgag actcaacgta aacgacttca cgaatgacat cctggctaga
     6301 aaaagtagcc aaactcttgc cgttaaagta gtggtagtca ttatcctccg gaataagacc
     6361 atcactaaca agttggtcga taagagtatt tccttttttg gtgcgagtat tgagtaactg
     6421 atagagattt tcaatcaagt caccatatat aatgggaaat ccagtttctt tacgaaaaac
     6481 gtcactatct ttgaagtcaa ccaaataaga aaagcctaaa agttgaaaag caacagtata
     6541 aaaaatatct gctgtcagtt cattttccga ttgaaaaaat gtcagcaggt ctgttttttt
     6601 atcagctgct aggatggaaa gtgggtagtt ggtgtcttga taagtgaaaa agaaacgacg
     6661 taaaaaggtt tcaagtgagt ctttgtgatt ggctgtattt tgtaaatcaa agccacattt
     6721 ttttagttca gataagatat tttcttttgg aaaattgata taactatatt gattaaaacg
     6781 catagaacct ccatatagaa tgacagttaa ggttattata tcaaaaaaaa agcagaaagg
     6841 gaattgttaa cttcaaaagg aaataatcca ataaaaatga ataaagtact aaattcagta
     6901 tagagaacag agtaacaata agaataatag atagggtata aaagttctag gagatttata
     6961 ttat
//
LOCUS       DAVGJL010000103         6839 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.104, whole genome shotgun sequence.
ACCESSION   DAVGJL010000103 DAVGJL010000000
VERSION     DAVGJL010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6839
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.104"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(<1..817)
                     /locus_tag="VVE42_001688"
     CDS             complement(<1..817)
                     /locus_tag="VVE42_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HEU3073848.1"
                     /translation="MKIKEIKKVTLQPFTKWTGGKRQLLPVIRELMPKTYNRYFEPFV
                     GGGALFFDLAPKDAVINDFNAELINCYQQIKDNPQELIEILKVHQEYNSKEYYLDLRS
                     ADRDERIDMMSEVQRAARILYMLRVNFNGLYRVNSKNQFNVPYGRYKNPKIVDEELIS
                     AISVYINNNQLEIKVGDFEKAIVDVRTGDFVYFDPPYIPLSETSAFTSYTHEGFSFAD
                     QVRLRDAFKRLSDTGAYVMLSNSSSALVEELYKDFNIHYVEATRTNGAKSSSRG"
     gene            complement(974..1378)
                     /locus_tag="VVE42_001689"
     CDS             complement(974..1378)
                     /locus_tag="VVE42_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEU3073849.1"
                     /translation="MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VDITNQEGRNVCKATFTMFVTGQRSEERQVRI"
     gene            complement(1448..2929)
                     /locus_tag="VVE42_001690"
     CDS             complement(1448..2929)
                     /locus_tag="VVE42_001690"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEU3073850.1"
                     /translation="MILVDKFVTHVISESSFEEMDRIYLTNRVLARVGEGVLEVETNL
                     DKLIDLKDQLVEEAVRLETIEDSQTAREILGTELMDLVTPCPSQVNRDFWEAYAYSPE
                     QAIEDFYQLSRKNDYIKLKAIAKNIAYRVPSDYGELEITINLSKPEKDPKEIAVAKLV
                     QASNYPQCQLCLENEGYHGRVNHPARSNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVEQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKTFRFAGFEQVKAGIIKWPMSVLRLTSDSKEDLINLADKIFQEWRQYSDSSVQ
                     ILAETDGTPHHTITPIARKRDGQFELDLVLRDNQTSAEYPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKAL
                     AIVKDSVGAIFARVLEDAGVYKQTEQGQTAFMRFVEHVGILLD"
     gene            complement(2948..4126)
                     /locus_tag="VVE42_001691"
     CDS             complement(2948..4126)
                     /locus_tag="VVE42_001691"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEU3073851.1"
                     /translation="MTQHLTAETLRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPAAISLGTYGAARKRDDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGAGLSSSASLELLTGVVAEHLFDLKLERLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVALDIQTLGELDEWAFDQYSYLIKDENRLKRAR
                     HAVLENQRTLKAQAALQAGDLETFGRLMNASHVSLERDYEVTGLELDTLVHTAWAQEE
                     VLGARMTGAGFGGCAIALVQKDTVEAFKEAVGKHYEEVVGYAPSFYIAEVAGGTRVLD
                     "
     gene            4290..5297
                     /gene="galR"
                     /locus_tag="VVE42_001692"
     CDS             4290..5297
                     /gene="galR"
                     /locus_tag="VVE42_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HEU3073852.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAM
                     KQVVDYFLSQGMDRIGILTGLEETTDQEEIIQDKRLENFKNYSQARGIYHDELVFQGR
                     FTAQSGYDLMKEAIQSLGDQLPPAFFAASDSLAIGALRALQEAGISLPDRVSLISFND
                     TSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHGRKIPSLTMLGTRLTLRESTLNQ
                     E"
     gene            complement(5343..6335)
                     /locus_tag="VVE42_001693"
     CDS             complement(5343..6335)
                     /locus_tag="VVE42_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU3073853.1"
                     /translation="MDLRPQIIEADDVIIRVVRACVCGSDLWRYRNPETKAGHKNSGH
                     EAIGIVEEAGEAITTVKPGDFVIVPFTHGCGECDACLAGFDGSCDNHIGNNLGGDFQA
                     EYIRFHYANWALVKIPGQPSDYTEGMLKSLLTLADVMPTGYHAARVANVQKGDKVVVI
                     GDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATAVVAERGQEGITKVREIL
                     GGGADAALECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRALGSTFMQNISVAGGAA
                     SATTYDKQFLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDERKTIKSMIVIE"
     gene            complement(6370..6723)
                     /locus_tag="VVE42_001694"
     CDS             complement(6370..6723)
                     /locus_tag="VVE42_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU3073854.1"
                     /translation="MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
BASE COUNT         2018 a         1641 c         1230 g         1950 t
ORIGIN      
        1 ttcctcgact tgaagatttt gctccattag ttcgggtagc ttcaacataa tgtatattaa
       61 aatccttata caactcctct actaaagcac tagaagaatt tgataacata acataagctc
      121 ctgtatcact caatctctta aaggcatctc ttaatcttac ttgatctgca aaagagaatc
      181 cctcatgagt ataagacgta aatgcactcg tctcagacaa tggaatatat ggagggtcaa
      241 aatacacaaa atctcctgtt cgaacatcta caattgcctt ttcaaaatct cccactttaa
      301 tttctagttg attgttattt atataaactg aaatagcaga tatcaattcc tcatcaacaa
      361 ttttaggatt cttataacgt ccatatggaa cattaaattg attcttagaa ttcacacgat
      421 atagaccatt aaagttcact ctcaacatat atagaatacg tgcagctctt tgtacttcgg
      481 acatcatatc tattctttca tcacgatctg cagaacgtaa atctaaataa tattcttttg
      541 aattatattc ctgatgaact ttcaaaattt caatcaattc ttgaggattg tccttaattt
      601 gttgatagca atttattagt tcagcgttaa aatcattaat aactgcatct ttaggagcca
      661 aatcaaaaaa taaagctcca cctccaacaa aaggttcgaa atacctgtta taggttttag
      721 gcattaattc tctaataaca ggcaataatt gtcttttacc acctgtccat ttcgtgaacg
      781 gttgtaaagt aactttcttt atttctttta tcttcatgct tttaattata ccatagaaat
      841 taaaacacaa ctcttgtatt ttctaccaca ttactaaatt ttctatttta gaattaaatc
      901 atatcaatat caaaaaatga gtaaggcaaa tcttataaat tccaaaattc acctcactct
      961 attaaattac tctttatatc cttacctgtc tttcttctga ccgctggcct gtgacaaaca
     1021 tagtaaaggt tgccttacag acatttctgc cctcttgatt ggtgatatcc acatccatca
     1081 cacaggttgt acgaccttga tggacacatt ctcctttaat ggtcaataca tcgtcgagtt
     1141 ttcctgcctt aaggtagttg atagaggatt ggagtgtcac cccatcaagt cccagcgaga
     1201 taaccaccaa accactgatt tggtcacata gcgtgaatag atagccacca tgggcattgc
     1261 catagtagtt gagcgacgag ttcactactt tggtcgtcac cacaacgtga ccatctctca
     1321 ttttttcaat ttcgtaattt tcaaaggcag atatagcgtc aaaatgaaag tctttcatcg
     1381 tttcctcctg atatttcaaa cgacactatg atactacttt ttataggact gtgcaaggag
     1441 aaagctccta gtctagtaaa attccgacat gttccacaaa gcgcataaag gctgtctgcc
     1501 cttgttctgt ctgcttgtag actcctgcat cctcaagtac acgcgcaaag atagcaccca
     1561 cagagtcctt gacgattgca agggcttttt ctttatccgt taggtctgga tgttgggatt
     1621 tgagttggtc tgcccactcc tgatgataat cggcaactgt aacagcttct cctacaagat
     1681 agctagcgac ttgctccact tcttctttta gacgtggtgg caagattgcc aagcccatga
     1741 cctcaatcaa gccgatattt tccttcttga tatgttggac atccttgtgg ggatgataga
     1801 taccatcagg atactctgct gaagtctgat tgtctcgcaa gaccaagtcc aactcaaact
     1861 gtccatcacg cttacgcgct attggtgtga tggtgtgatg cggtgtcccg tctgtctctg
     1921 ctaaaatctg cacactagaa tctgaatact gacgccattc ctggaaaatc ttatcagcta
     1981 ggttaatcaa atcctctttg gaatccgaag tcaagcgtag gacagacatt ggccacttga
     2041 taattccagc cttgacctgc tcaaaaccag caaatcggaa ggtcttttgc aagggagcca
     2101 attccatagg aaatacgtga cggcctccct gataatgatc atgagttaga atagagcccc
     2161 ccacaatcgg caggtcggca ttagatccag caaaatatcc tggaaactgc tctacgatag
     2221 ccaacagacg ttcaaaactc tgacgactaa tggccatggg acgatgctgg ccatctaaaa
     2281 agatacaatg ctcattaaag taagcatagg gcgaatactg gaacccccat tcctgaccaa
     2341 ccatttcaaa acggataatc cggtgattgc tgcgagctgg gtggttgacc cgaccatggt
     2401 aaccctcatt ctctaaacaa agttgacact gaggataatt actagcttgc accaacttgg
     2461 ctaccgcaat ctctttggga tccttttcag gcttagagag attgatggta atttcaagtt
     2521 ctccataatc agatggaaca cgataagcga tatttttagc aatggccttg agtttgatgt
     2581 agtcattttt ccgactgagt tgataaaaat cctctatcgc ttgctcagga gagtaggcat
     2641 aggcttccca gaagtcacga ttgacctgac tcggacaagg agtcaccaag tccatcagtt
     2701 cagtaccaag gatttcacgc gcagtctgac tatcctcaat cgtctctaat cgaacggctt
     2761 cctcaactag ctggtccttg aggtcaatca atttatccag attggtctca acttccaaaa
     2821 caccttctcc cactcgtgcc aaaacacgat tggtcaggta gattcgatcc atttcctcaa
     2881 atgaactttc agaaatgaca tgtgttacaa atttatctac taagatcact atagagcctc
     2941 cttttgacta gtcaaggacg cgagtgccac ctgcaacttc agcgatatag aagcttggag
     3001 cgtatccaac tacttcctcg tagtgtttgc ctacagcttc cttaaaggcc tcaacagtat
     3061 ctttttgaac caaggcaatg gcacagccac caaaaccagc ccctgtcata cgagcaccga
     3121 gaacttcttc ttgtgcccaa gctgtgtgaa caagagtatc caattccaaa ccagttactt
     3181 cataatcacg ctccagagaa acgtgtgacg cattcatcaa acgaccaaat gtttccaaat
     3241 cacctgcttg aagggctgct tgagctttaa gggtacgttg gttttcaagc acagcatggc
     3301 gagcacgttt caaacgattt tcatctttaa tcagatagct atattggtca aaggcccact
     3361 cgtccaattc acccagggtc tgaatatcca aggcaacttg caattcttcc actgcttttt
     3421 cacactcagc acgacgttca ttgtatttag agtccgccaa ttcacggcgt ttgttggtgt
     3481 tcatgataac aacgacattg tccttcaaat caagtggcac caaatcatac tctaaggtgt
     3541 tggtatcaag gtaaatagca cgttgatcag cccccatacc aatagcaaac tggtccatga
     3601 taccagagtt gactccgata aagttatttt ctgtttgttt tccgatttta accaaatcga
     3661 gacgctctaa ttttaaatca aagagatgct ctgccactac ccctgtcaag agctccaagg
     3721 atgctgaaga agacaaacca gcaccatttg ggatattccc ataaacataa aaatcaaaac
     3781 ctttgtcaat cacgtgccca gcttcttgca agaaatgaag gacacctttt ggataattgg
     3841 tccagttgtg ctctttttca aacttgaggt cagcgagagg cacttcgata atgcccttgt
     3901 cctcaaagtt agctgagtag aaacgcaaaa cttggtcgtc acgcttacga gctgcaccgt
     3961 aagttcccaa ggaaatagca gcaggaaaaa cgtgcccacc gttgtagtct gtgtgttcac
     4021 caatcaaatt gatacgacct ggtgaaaaga aagtttggtc tgcttcttga ccaaaaacag
     4081 caagaaagtc tttgcgaaga gtttcagcag taagatgttg tgtcatatga tttctccttt
     4141 gactgtccgt taagccacct taacgtgtaa tcgttttctt ctattgtatc caagggattt
     4201 cccaaggtca atccttttta ctaaaatttt actaaactat cagtaaaaag cagaaaaata
     4261 tgatatacta acctaatcaa ggaggattta tggctacctt aaaagacatt gcacagctag
     4321 cctctgtctc tatcgcgacc gtatcccgtg tcctcaaccg cgaccagagc ctatctgtta
     4381 cagaagaaac cagacaccgt attttaaccg ttgctgaaga gctgggctac accaagcacc
     4441 tcaagacagg cgagtcccat aaacccaagc aaaagattgc cattattcaa tgggtcagcg
     4501 aacaagggga gctagacgac ctctactact accagattcg cctaggaata gaaaaaagag
     4561 cccaagagtt ggactacgat atcttgcgct attttaatga ccaccctttt accctaagcg
     4621 aggaagtgat tgggattctc tgcatcggaa agtttagtcg agctcagatt tctgcctttg
     4681 aagaatacca aaagcctctt gtatttctag acagcgatac actttccctg ggacatacct
     4741 gtattatcac ggatttttac actgctatga aacaggttgt cgattatttc ctcagtcaag
     4801 ggatggaccg catcgggatt ctaacagggc ttgaggaaac aacagaccaa gaagaaatca
     4861 ttcaggacaa gcgtctagaa aacttcaaaa actacagtca agcgagggga atctatcatg
     4921 atgaactggt ctttcaagga agatttactg cccagtctgg ctatgactta atgaaggagg
     4981 ccattcagag cttgggagac caacttccgc cagcattttt cgcagccagc gatagtttag
     5041 ctatcggtgc cctccgtgcc ctccaagaag ctggaatcag cctgccagat cgcgtcagcc
     5101 tcatttcatt caacgacacc agtctgacca aacaggtcta tcctccactc tctagtatta
     5161 cagtttatac tgaagaaatg gggcgagcag gtatggatat tcttaacaag gaagtcctcc
     5221 acggtcgcaa aattcctagc ctgaccatgc tgggaaccag actgacatta agagaaagta
     5281 ccctaaatca agaataggat aacataaaaa acgaatagag ttctaaaact cctattcgtt
     5341 ttttattcga ttacaatcat agacttaatg gtcttacgtt catccatatc tttgtaggct
     5401 tggtcgatat cttccagttt ataacttgaa gtaaagacgc gacctggatt gatatcacca
     5461 tcaaggacgg cttttagtaa aaattgctta tcgtatgttg tagcagaagc tgccccacct
     5521 gctacagaga tattttgcat aaatgtcgaa ccaagagcac gattattata gtgtgggact
     5581 cctacaaagc ccatacgccc tccattatga agaacaccta gcgcctgttc tatagcagcc
     5641 tccgtaccaa cacattcaag tgctgcatct gctcctccac cgaggatttc acgcaccttg
     5701 gtaattcctt cttgaccacg ttctgcaaca acagctgtcg cacctgactc catagccatc
     5761 ttttgacggt cttcatgacg gctcataagg ataatttgtg atgctccacg catcttagcc
     5821 gcgatgacag cacattgacc aacagcccca tcaccgataa caacaacctt gtcccctttt
     5881 tgaacatttg caacacgcgc cgcatgatag cctgtcggca tgacatctgc aagagtcaaa
     5941 agggacttga gcatcccttc tgtatagtca gaaggttgac cagggatttt aaccagcgcc
     6001 cagtttgcat agtggaagcg aatatattct gcctgaaaat caccccccaa attattgcca
     6061 atatgattgt cgcaagaacc gtcaaatcca gcaagacagg catcacactc accacatcca
     6121 tgtgtaaaag ggacaatcac aaaatcacct ggtttcaccg tcgtaatggc ttccccagct
     6181 tcttcaacaa tcccaatcgc ttcgtgtcca ctatttttgt gtccagcttt cgtttctgga
     6241 ttacggtacc tccataaatc tgaaccacaa acgcacgcac gaaccacacg aataatcaca
     6301 tcatccgctt ctattatttg cggacgcaag tccaacctga cctgcctttg tatatactgc
     6361 tgatttcatt taaaattttc cttccttata aagtttaatt ttgagattta aacgatttaa
     6421 agctgtctgt agctgagaca agcgctcctc taatttttgc ttttcctctt ctaaaatacc
     6481 aagcctctcc tctctcgttt catctccctt ttggtagagc gacatatagt caactaaact
     6541 atctacagag acacccgccg aacgaaaaca cttaataaat tccagcgctt cgatatcctg
     6601 atcttgaaaa tcacgaatcc cagtagcagt acgagtaatc ggtggcacaa gaccaacccg
     6661 ttcataatac cgaatcgtat ccgctgaaat tcccaacaaa tcactggcag atttaatatt
     6721 cacacgctaa cctcctctca tttcattcac tgaattcacg ctattctaac tcacttatct
     6781 tattatataa ctttgagttg actccaagtc aagcattttg tatctaatcg aaaaatttt
//
LOCUS       DAVGJL010000104         6510 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.105, whole genome shotgun sequence.
ACCESSION   DAVGJL010000104 DAVGJL010000000
VERSION     DAVGJL010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6510
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.105"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(51..890)
                     /locus_tag="VVE42_001695"
     CDS             complement(51..890)
                     /locus_tag="VVE42_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU3073855.1"
                     /translation="MTKIKIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLK
                     EEGKFLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQ
                     GASLSTADVTVVDSSFTDQALKFQVVEAAKLAQEGKDMEAILSHVEEVKNHTELYIGV
                     STLENLVKGGRISRVTGLLSSLLNIRVVMQMKDHELQPMVKGRGTKTFKKWLDELITS
                     LSERAVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIR
                     YHS"
     gene            1032..1880
                     /locus_tag="VVE42_001696"
     CDS             1032..1880
                     /locus_tag="VVE42_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEU3073856.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSAWLAFFEHIPLHIDLQ
                     GDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCIL
                     MLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDL
                     GRGVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEG
                     FTFEKE"
     gene            complement(1973..2890)
                     /locus_tag="VVE42_001697"
     CDS             complement(1973..2890)
                     /locus_tag="VVE42_001697"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEU3073857.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKDYFDGEVIIVPPVEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRTIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVDQLKADEEVTRNWS"
     gene            3328..3533
                     /locus_tag="VVE42_001698"
                     /pseudo
     CDS             3328..3533
                     /locus_tag="VVE42_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(3844..4137)
                     /gene="rpmA"
                     /locus_tag="VVE42_001699"
     CDS             complement(3844..4137)
                     /gene="rpmA"
                     /locus_tag="VVE42_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEU3073858.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(4154..4498)
                     /locus_tag="VVE42_001700"
     CDS             complement(4154..4498)
                     /locus_tag="VVE42_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEU3073859.1"
                     /translation="MIQAVFERAEDGELRSAEITGHAESGEYGLDVVCASVSTLAINF
                     INSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENS
                     SEFVQTRVITEN"
     gene            complement(4514..4828)
                     /gene="rplU"
                     /locus_tag="VVE42_001701"
     CDS             complement(4514..4828)
                     /gene="rplU"
                     /locus_tag="VVE42_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEU3073860.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYIEKLNVEAGQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     misc_feature    complement(4865..4952)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            complement(5047..5646)
                     /locus_tag="VVE42_001702"
     CDS             complement(5047..5646)
                     /locus_tag="VVE42_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEU3073861.1"
                     /translation="MTFCAGYVDAYTFIVRGNTLVAGQAGNVVFLSVELIQNNVSDIR
                     DKLLTLIAFMMGVFLLTIYKEKLRIVKKSILSLIPLAILSIIIGFVPQTVDNIYLVPP
                     LAFCMGLVTTAFGEVSGIAYNNAFMTGNIKRTMLAFGDYFRTKHTPFLREGFIFVSLL
                     SSFVLGVVFSVYLTIFYHEKTILGVPIMMSIFYLSMLFA"
     gene            6117..6416
                     /locus_tag="VVE42_001703"
     CDS             6117..6416
                     /locus_tag="VVE42_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEU3073862.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVVSIDFNPAVVDPEDLETLSDMTVQAINSALEQIDETTKKKLGAFAGKLPF"
BASE COUNT         2069 a         1314 c         1084 g         2043 t
ORIGIN      
        1 ccttttaggt caagttaaaa acgctatttc ttcccatttt atttattttt ttaggagtgg
       61 taacgtatca aaatagccca agcgttctca cccgtgtgag tttgaataat ggaacccgtt
      121 tccaaaacag aaattggctt ttcaacataa gcttgtaagc tttctttcat ctcttttgcc
      181 caatcatcac taccagaata tgaaattcca atctctgcta cagcacgttc agaaagcgat
      241 gttatcaact catctaacca ttttttaaat gttttagttc cacgaccttt aaccattggc
      301 tgcaattcat ggtctttcat ttgcatgaca acacggatat tgagaagaga gctcaacaag
      361 ccagttacac ggctaattcg tccacctttg acaagatttt ccaaagttga aacaccaata
      421 taaagctctg tatggttttt aacctcttct acatgagata aaattgcctc catatcttta
      481 ccttcttgag ctaacttcgc agcctcaaca acttggaatt tcagggcttg gtcagtgaag
      541 gaactatcaa caacagtcac atctgcagta gataggctag caccttggcg tgctgcttct
      601 accgtacccg aaagagcatg ggacatatga atagcaagaa tctggccacc atctttgcat
      661 aggtcttcaa aaatctcagc aaagacacct acaggtggct gacttgtttt cggaagattc
      721 ttactttctt gcatcaactg aagaaattta ccttcttctt tcaaatccgc atcagaataa
      781 acaacattat caatcattac agataatgga acaattgtaa tatctaattg ctttactagt
      841 tcaggttcaa tagtaacaga tgaatcggtt acaatcttaa tttttgtcat agtatcaatc
      901 tttctatttt aggattcaga ttggtttcct tacttttaat tatatcaaaa aaaagattaa
      961 aaatcctaat ggagtcaatc aaattttccg taaaatttga tataatcaac ttataagaaa
     1021 agaggtgtcc tatgattaaa aaaatttacc ccatttttac cattttacta ggtgctgcta
     1081 tttatgcttt tggactgact tattttgtag ttccccatca tctctttgaa ggaggggcga
     1141 caggcattac cctcatcacc ttttatcttt ttaaaatccc tgtttccctc atgaacctgc
     1201 tgattaatat tccccttttc atcctagctt ggaagatctt tggagccaaa tccctctatt
     1261 ctagtttact aggaacctta gctttgtccg cctggttagc tttttttgag catattcccc
     1321 ttcatattga tcttcaaggt gatttactaa tcacagccct tatagcggga atcctattgg
     1381 gaattggcct tggaattatt tttaatgctg gaggtacaac tggcggaact gatattctag
     1441 ctcgtattct caacaaatac actcatatat ccataggaaa actgctcttt atcttagatt
     1501 tttgtattct catgttgatt ctcctaatct tcaaggattt gagattggtt tcctacacgc
     1561 ttttgtttga ttttattgtt tctcgtgtta ttgatttgat tggtgaagga ggatatgccg
     1621 gcaaaggctt tatgattatc acaaaacgtc ctgaccaact tgctaaggcg attaatgatg
     1681 acctcggaag aggtgttact tttatttctg gtcaaggcta ctatagtaaa gaaaatttga
     1741 aaatcatcta ctgtattgtc ggaagaaatg aaattgtgaa aacgaaggaa atgattcatc
     1801 gaatcgatcc tcaagccttt ataactatta cagaagccca tgaaatccta ggagaaggct
     1861 tcacctttga aaaagaataa aaagaggtaa tgtcgtgacc tcaaaagtta gactaaatca
     1921 tctatctttt gggttacaga caacctcttt tttgttttat ttactcaagc tcttaagacc
     1981 aattccgagt tacttcttca tcagccttta actgatccac taattggtca actgagtcaa
     2041 atttggtcat atcacgaatg cgatcaagcc aataaaccat gacggtttcc ccataaatat
     2101 cttgattaaa atcaaaaata ttgacttcaa aacgtgcttc ttctccatca aaggtcacat
     2161 ttttcccgac actagccata gcacgatact tctgtctttg aatctcaaca tcaacgacat
     2221 aaacgccatc tgctggcata taagtacggt ctaaaagcac taaattcgct gtcggataac
     2281 caattgtacg accacgagca ttaccatgaa ctaccatacc tcttgatgga agcggtgccc
     2341 ccaaaagttt tcctgcttct ttcacatttc catctaaaat agcttgacgg atacgagttg
     2401 aactaatctt tcctttctca tcttctacag gtggaacaat gataacttct ccatcaaagt
     2461 aatcctttaa atcttctgct gtttttttgt cagaaccaaa tgtataatca aaacctgcaa
     2521 caataatttt ggcattcata gccttgatat aagttgcaaa gaattcttgt gcagtgagac
     2581 tagcgaattg actactaaaa tcaaggagat ataattcttc tacaccttcg cgctttaatt
     2641 ttctttcacg ttcagcaggg ttcaaaatat gcaaaaacaa atctggatga taaggctcta
     2701 aagcgatctt tggagattca ttaaaggtca taacgacgat aggcaataaa tcctttctcg
     2761 cagccttgtt ggcaacacga aataattctt gatgcccctt atgtatgcca tcaaaatagc
     2821 cgagaacaac gactgaatca gatggtgtgc caatatcttt ttggtttttt ataggaatag
     2881 taataatcat aaaataatta tatcatagcg atagctattt ctggaacaga aaatctgaaa
     2941 tgttgttttt ttcacatgaa gtgtacctgt tttcaaaagg cactttattc tatcgttgct
     3001 taactatgaa ctttgcaata ttcttctcaa aaacttgtag gacatcttca aaattttgca
     3061 aggagtgatt agacttgttc ggtaaccata aagtgtcata ctatgcttat gtatgaaaaa
     3121 gcaatgcaac taactcctga gaactttaaa ttactaattg gtgccgaaaa ggtagaattt
     3181 agaatcgagg tacacctatg gctataaaat ttacaaaatg agacaacttg ggcaagatgt
     3241 ttgaagaatt tcctaaactc cctgatttga agcaagtcac tttccctgat gacaaagaaa
     3301 aaagccaaag caataaagaa aaactagatg actgctttcc aacaactccc atctagtgtg
     3361 cttcagactg ggctattttt ctttccatct gttagcttgg attctcagac cgtttcagct
     3421 aaagaatatc ttttccttta tcagaaggaa cggctcaagc cattcagaca agtgaaggga
     3481 cgacaagcca atatttgaaa ccaggtagca gttcttatag tcaattgaaa taaaatctga
     3541 agaaatcgag taggaaactt aaatcaattt ttagcagtgt tctattccag attcatactc
     3601 aatgaaaatt aaagtgcaaa ctaggaagtt agccgcaggc tgtacttgag tacggcaagg
     3661 tgaaactgac gtggtttgaa tttgattttc gaagagtatc aatccactat acatattaaa
     3721 aagtatgggt accaaaacaa aaaaacaggc tctccgaaaa ctcggaaagc cttattttaa
     3781 gctgtttcta gcttcctcac ctttacattt caaggttcgg gataaaaggt tcaatggacc
     3841 tttttattta gcgattgggt aaacagaaac ttgtttttta tcgcgtcctt tacgttcaaa
     3901 gcgtactacg ccttcaactt tagcgaacaa agtatcgtct ccaccacgac caacgtttac
     3961 acctggatag atgtgtgtac cacgttgacg gtaaaggatt gatccacctg ttacagtttg
     4021 tccgtcagct gctttagctc caagacgttt cgcttgtgaa tcacgtccgt ttgatgtaga
     4081 acctccacct tttttgtggg cgaaaagttg caagttgtta agagtcattt ttaacataat
     4141 gttttcctcc gtgttagttt tctgtgataa ctctggtttg gacgaactca gaagagttct
     4201 ccgataagtt tgccatacct aagaaaaatg attcaaagaa taactgggtc atttctctct
     4261 ggtgtgaagg aagatctttt ggtatttcaa ccatcagata gccaccttca tcttcgttta
     4321 attctaggat tggttcatag cctgcaaatt tctcaataga attgataaag ttaatggcaa
     4381 gcgtagaaac cgatgcacac acgacatcta agccgtattc gccactctcg gcgtgtccag
     4441 taatttccgc actcctcagc tcgccatctt cggctctctc aaagactgct tgtatcatgt
     4501 gttctcctta aaattaagcg ttaattgcgt tgatgacaac ttttgtatat ggttgacggt
     4561 gaccttgttt acggtggcta ccttttttag gtttgtactt gtaagtaaca actttctttt
     4621 gttttccttg tttttcaaca gttccaacta cagtagctcc agcaacaagt ggagttccga
     4681 caacagtgtt ttcaccacca acaagaacaa cttcgttaaa agtaacttct tgaccagctt
     4741 caacgttcaa tttttcaatg taaactgctt gaccaacttc aactttaact tgttttccgc
     4801 cagttttgat aattgcgtat gtgctcatta tgcacctcct atgattttta tgggtttccc
     4861 cgattttttt gtgaagactc gcctagcatc gtgggacaaa ccacttaaac ttgaataatc
     4921 aagcaacgat gttcgtgcgg ttgcacagga tcgtgcatag tcaactcttc aagtgtagca
     4981 tatttcctat tttcttacaa gtaataacac ctaaaatcaa gctttttctt ttactttttt
     5041 ctgccactag gcaaaaagca tactgagata aaaaatgctc atcataatag gaacaccaag
     5101 aatggtcttt tcatgataga aaatcgtcaa atagactgaa aagacaacgc caaggacaaa
     5161 actactaagc aggctaacaa atatgaaccc ttcacgcaaa aaaggagtgt gcttagttcg
     5221 gaaataatct ccaaaagcta gcattgttcg tttgatattc cctgtcataa aggcattatt
     5281 gtaggcaata cccgaaacct caccaaaagc agttgtcacc agtcccatac agaaggccaa
     5341 gggcggcact agatagatat tatctacagt ttgtggcaca aaaccaataa ttattgatag
     5401 gattgctaaa ggaatcaagg acagaataga ttttttcaca attctcaatt tttccttata
     5461 aatcgttaat aaaaagactc ccatcataaa cgctattaag gttaaaagct tatccctaat
     5521 atccgaaaca ttattttgta tgagctctac cgaaagaaag acaacatttc cagcttgtcc
     5581 agctacaagg gtattcccgc gaacaataaa agtgtaagca tccacatatc cagcacaaaa
     5641 cgtcaaaaaa gtgctaacct tttagactga cgtgatattt ttcttatagg taataacctc
     5701 attttccctc ccattgtatt ttctcttaga aatattgtac cattttcttt ctaaaaaatc
     5761 gtaggctacc atttagattt tactattagc ataaaaataa taatagacaa ctatttatcc
     5821 aaaaatagat agaggtaaca tgtttgcaaa caaagcatac gaacctttag taaaatcatt
     5881 tccatgaaac tagaatagag cccctcttag caaaaatcat tattttaatt tatttctaat
     5941 cactccttga cataaataac tctcaccaat aaaagactat gtcttaaaaa aatggtataa
     6001 taaaatcaat acttgggctt gatggctatg ctactaataa caattaggag agaaaatcag
     6061 gcacttgtta acaacaagga ttatcccctt gagatgaaag gaactttaga aatcttatga
     6121 tgaacatgca aaacatgatg cgtcaagcac aaaaacttca aaaacaaatg gaacaaagcc
     6181 aagctgaact tgctgctatg caatttgttg gcaaatctgc tcaagatctt gtccaagcaa
     6241 ccttaactgg cgataagaaa gttgtcagca ttgatttcaa tccagctgtc gttgacccag
     6301 aagaccttga gactctttct gatatgaccg ttcaagccat caactctgct cttgaacaaa
     6361 tcgatgaaac tactaagaaa aaactgggtg ctttcgctgg gaaattgcca ttctaaaaac
     6421 aaggagctag aacaatgctt gtcgataaca aaggctaaga aaggtgcaaa aattataaaa
     6481 aacgcatagt atcaagcaac ctaaaacttt
//
LOCUS       DAVGJL010000105         6415 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.106, whole genome shotgun sequence.
ACCESSION   DAVGJL010000105 DAVGJL010000000
VERSION     DAVGJL010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6415
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.106"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            39..1712
                     /locus_tag="VVE42_001704"
     CDS             39..1712
                     /locus_tag="VVE42_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEU3073863.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALGDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLWIGI
                     SKERITIEKLIELTEVSRNTVLNDLNSIRYQLTLEQYQVILQVSKSQGYHLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQVLPYLLLSCHNVEQYQERHQDIEKEFSLIRKRIEYQVSKKLGERLF
                     QKFEISLSGLEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKALLFRKTYGIFSKNPLTKQIRSKYGELFLVTRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTPSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNESKSFSENLSSLISSYIVDSKLASKFQEEVQTLINQEIVVQAFLEDI"
     gene            1824..2915
                     /gene="ulaG"
                     /locus_tag="VVE42_001705"
     CDS             1824..2915
                     /gene="ulaG"
                     /locus_tag="VVE42_001705"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEU3073864.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNPKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            3029..5005
                     /gene="tkt"
                     /locus_tag="VVE42_001706"
     CDS             3029..5005
                     /gene="tkt"
                     /locus_tag="VVE42_001706"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU3073865.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVADYKEAHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDANRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVSAAKEL
                     ASQGEKIRVVSMPSTDVFDKQDAAYKEEILPNAVRRRVAVEMGVSQNWYKYVGLDGAV
                     LGIDTFGASAPAPKVLAEYGFTVENLVKIVRNLK"
     gene            5122..5421
                     /gene="yajC"
                     /locus_tag="VVE42_001707"
     CDS             5122..5421
                     /gene="yajC"
                     /locus_tag="VVE42_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEU3073866.1"
                     /translation="MNPNITFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVDGVYLTFELAAIKTVLPLKETASLEGAIEK"
     gene            5468..5896
                     /locus_tag="VVE42_001708"
     CDS             5468..5896
                     /locus_tag="VVE42_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEU3073867.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQQYEIPYDKNKTSLQISKEDFEAFDYIIGMDASNISDLRQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            5898..6308
                     /locus_tag="VVE42_001709"
     CDS             5898..6308
                     /locus_tag="VVE42_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073868.1"
                     /translation="MENLTNFYEKYRVYLTRPRLELLAVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
BASE COUNT         2032 a         1130 c         1354 g         1899 t
ORIGIN      
        1 attgctgtga tatcaataga tttgggggat tttttaatat ggtactggat aaggcaagtt
       61 gtgatttgct tcaatatttg atggatcaag aaacgtccaa aacgattatg gcgatttcga
      121 aagatttgaa agagtcaaga aggaaaattt attatcacat tgacaaaatc aatgctgctc
      181 tgggtgacga ggcgcttcac atcattagta ttccacgaat tggtattcac ttaacggaag
      241 agcagagaga tgcttgttgt aaactattat cggaagtaga ttcgtacgat tatatcatga
      301 gtgcgcatga acgtatgatg ataatgttac tatggatagg tatttctaaa gaacgtatta
      361 cgattgaaaa attgatagag ttaacagagg tatctaggaa tactgttctc aatgatttga
      421 atagtattcg ttatcaacta actttggaac aatatcaggt gatcttgcaa gtgagcaagt
      481 cacagggata ccaccttcat gcccaccctc ttaataaaat tcagtatctt caatcgcttc
      541 tatatcatat ttttatggaa gaaaatgcca cttttgtatc tattttagaa gataagatga
      601 aagagaggtt agatgatgag tgtttgcttt ctgttgaaat gaaccaattt tttaaggaac
      661 aggttccttt agttgaacaa gatttaggga agaaaataaa ccatcatgaa ataactttta
      721 tgttgcaggt tctaccttat ttgctgttaa gctgtcataa tgttgaacag tatcaagaaa
      781 gacatcagga tatagagaaa gaattttctt tgataagaaa aagaatagag tatcaggtgt
      841 ctaagaaatt aggagaacgg ttgtttcaaa agtttgaaat ttctttgtca ggacttgaag
      901 tttctcttgt agctgttctc ctcctctcct atcgtaaaga tttggatatt catgcagaaa
      961 gtgatgattt tcggcaatta aaacttgctt tagaagaatt tatctggtat tttgaatcac
     1021 aaatccgaat ggagattgag aacaaggatg atttgttacg aaatttgatg atccactgta
     1081 aagccttgtt atttagaaag acttacggta ttttttctaa aaatcctcta acaaaacaaa
     1141 ttcgatccaa gtatggagaa ttatttttag tcactagaaa atctgcggaa attttagaag
     1201 gagcatggtt tattcggcta acagacgatg atattgccta tttgacgatt catattggag
     1261 gatttttaaa gtatacacca tcgtctcaaa aaaatatgaa aaaagtttat ctcgtttgtg
     1321 atgaaggtgt tgcggtttcg agacttttgc tgaaacaatg caaactttat tttccaaatg
     1381 agcaaattga cactgtattt acaacagaac aatttaagag tgtggaagat attgcacaag
     1441 ttgatgtagt gattactact aatgatgatt tggatagcag atttccgatt ttaagggtta
     1501 atcctatcct tgaagcagaa gatattttga aaatgctaga ctatcttaaa cacaatatat
     1561 ttcgtaatga gagcaaaagt ttcagtgaaa atctttctag tcttatttcg tcttatattg
     1621 tagatagcaa gttggctagt aagttccaag aagaggttca aacacttata aatcaagaaa
     1681 tagtagttca agcttttttg gaagatattt gaaggacagt ccaatgatga acacaaacct
     1741 gtgtttttct tggtcttttt tagtgttttg aagggtggta tactaatctc aaagataaca
     1801 attatatcca aaggaggcaa catatgccaa acgtcaaaga aattacaaga gagtcatgga
     1861 ttttagccac tttcccagag tggggaacat ggttgaacga agaaatcgaa gaagaagtcg
     1921 tacctgaagg caactttgcc atgtggtggc taggcaactg tggtacttgg attaagacac
     1981 cagctggtgc taacgttgtc atggaccttt ggtcaaatcg tggaaaatca accaaaaaag
     2041 tgaaagatat ggttcgtggg caccaaatgg caaatatggc aggtgttcgt aagttgcaac
     2101 caaacttgcg tgttcagcca atggttatcg atccatttgc tatcaacgaa ctagactatt
     2161 acttagtttc acacttccac agtgatcata tcgacccata cacagctgca gcaattctca
     2221 acaatcctaa gttagagcat gttaagttta tcggtcctta ccactgtgga cgaatctggg
     2281 aaggatgggg tgttccaaaa gaacgtatca tcgttgttaa accaggtgac actatcgaat
     2341 taaaagatat gaagattcat gcagtagaat catttgaccg tacttgcttg gtaactctcc
     2401 cagtgaacgg tgctgatgag acaggcggtg aacttgctgg cttggctgtt acagatgaag
     2461 aaatggctca aaaggctgtt aactatatct ttgaaacacc aggtggaacc atctatcatg
     2521 gtgcagattc tcacttctca aactattttg caaaacatgg taaagacttt aaaattgatg
     2581 ttgctttgaa taactatggt gaaaatccgg taggtatcca agacaaaatg acatctatcg
     2641 accttcttcg tatggcagaa aatctgcgta ccaaagtcat tatcccagtt cactatgata
     2701 tctggtctaa cttcatggct tctactaatg agattctaga actttggaaa atgcgaaaag
     2761 atcgcttgca atacgatttc catccattta tctgggaagt tggcggtaag tacacttatc
     2821 ctcaagatca acacttagta gaataccatc atccacgtgg ttttgatgat tgttttgaac
     2881 aagactctaa cattcaattt aaagctttgc tataatataa aaatattctt tggaggttat
     2941 ccgtatgttg catgatacgg ataattttca tataatagta aaatagaatg tgtgattcaa
     3001 taatcacctc aaatagaaag gaaattctat gtcaaatcta tctgttaatg caattcgttt
     3061 tctaggtatt gacgccatta ataaagccaa ctcaggtcat ccaggtgtgg ttatgggagc
     3121 ggctccgatg gcttacagcc tctttacaaa acaacttcat atcaatccag ctcaaccaaa
     3181 ctggattaac cgcgaccgct ttattctttc agcaggtcat ggttcaatgc tcctttatgc
     3241 tcttcttcac ctttctggtt ttgaagatgt cagcatggat gagattaaga gtttccgtca
     3301 atggggttca aaaacaccag gtcacccaga atttggtcat acggcaggga ttgatgctac
     3361 gacaggtcct ctagggcaag gtatttcaac tgctactggt tttgcccaag cagaacgttt
     3421 cttggcagcc aaatataacc gtgaaggcta caatatcttt gaccactata cttacgttat
     3481 ctgtggagac ggagacttga tggaaggtgt ctcaagcgag gcagcttcat acgcaggttt
     3541 gcaaaaactt gataagttgg ttgttcttta tgattcaaat gatatcaact tggatggtga
     3601 gacaaaggat tcctttacag aaagtgttcg tgaccgttac aatgcctacg gttggcatac
     3661 tgccttggtt gaaaatggaa cagacttgga agccatccat gctgctatcg aaacagcaaa
     3721 agcttcaggc aagccatctt tgattgaagt gaagacggtt attggatacg gttctccaaa
     3781 caaacaagga actaatgctg tacacggcgc ccctcttgga gcagatgaaa ctgcatcaac
     3841 tcgtcaagcc ctcggttggg actacgaacc atttgaaatt ccagaacaag tatatgctga
     3901 tttcaaagaa catgttgcag accgtggcgc atcagcttat caagcttgga ctaaattagt
     3961 tgcagattat aaagaagctc atccagaact ggctgcagaa gtagaagcca tcatcgacgg
     4021 acgtgatcca gtcgaagtga ctccagcaga cttcccagct ttagaaaatg gtttttctca
     4081 agcaactcgt aattcaagtc aagatgcctt gaatgttgtg gcagctaaat taccaacctt
     4141 tctaggtgga tcagctgacc ttgctcactc aaacatgact tatatcaaga cggatggact
     4201 tcaagacgat gccaatcgct tgaatcgtaa cattcagttt ggtgttcgtg aatttgcaat
     4261 gggaacaatc ttgaacggga tggcccttca tggtggactt cgtgtatacg gtggaacttt
     4321 cttcgtcttc tctgactatg tgaaggcagc tgtccgcttg tcagccttac aaggacttcc
     4381 tgtgacttat gtctttaccc atgattcaat cgcagttggg gaagatggtc cgactcacga
     4441 accagttgag catttagcag gtcttcgtgc tatgccaaat ctaaatgttt tccgtccagc
     4501 agatgcgcgt gaaacgcaag cagcttggta ccttgcagtg acaagtgaga aaacaccaac
     4561 tgcccttgtc ttgacacgtc aaaacttgac tgttgaagat ggaacagact tcgacaaggt
     4621 tgctaaaggt gcttatgttg tatatgaaaa tgcagccgac tttgatacca tcttgattgc
     4681 gacaggttca gaggttaatc ttgctgtctc agctgctaaa gaattggcta gtcaaggcga
     4741 aaaaatccgc gtagtcagca tgccatctac agatgtcttt gataaacaag atgcagctta
     4801 caaggaagaa attcttccaa atgcagtccg ccgtcgtgtt gcagtcgaaa tgggtgtaag
     4861 tcaaaactgg tacaaatatg ttggtctcga tggtgccgtt ctaggtattg ataccttcgg
     4921 agcctctgcc ccagcaccaa aagtattggc agaatatggc tttactgtag aaaatcttgt
     4981 aaaaattgtt cgaaacttga aataatccta aaaatcaggg cgtaagctct ggtttttctt
     5041 accagaaaag taaggtacaa tcttgtaaaa gtagctgaaa tttgatatag tagtcctatg
     5101 taaaagacaa aggagaaaac aatgaatcca aatattactt ttttaatcat gcttgtaggt
     5161 atgatggcct tgatgttctt tatgcaacgt tctcaaaaga aacaagctca aaagcgtatg
     5221 gaaagcttaa ataaactaca aaaaggctat gaagtgatta caatcggtgg actttatgga
     5281 acagtcgatg aagtagatac ggagaaggga acaatagttc ttgacgtaga tggagtttac
     5341 ttgacttttg aactagctgc tatcaagaca gtattaccgc tgaaagaaac agcttcacta
     5401 gaaggcgcaa ttgaaaaata agacgggatc acgaactccc gtttttctat aaaagaaagg
     5461 aaatgggatg aaaaaattag tctttgtctg tctgggaaat atttgccgta gccctatggc
     5521 cgagtttgtt atgaaatcaa tgacagataa ctacgaaatc caaagtcgag caacttcctc
     5581 ttgggaacat ggcaatccga ttcataaggg gactcaggga atttttcaac agtatgagat
     5641 tccttatgac aagaacaaga catcgcttca gattagtaag gaagattttg aagcctttga
     5701 ttatattatc ggaatggacg cttcaaatat ttctgactta cgtcagatgt gtccagtaga
     5761 ctgtcaagat aagatttact cattttcatc tgaaagtgtt ccagaccctt ggtatacagg
     5821 agattttgaa gaaacctatc gacgtgttca agagggctgt caagcttggt tagaacgttt
     5881 agaaaaggag agttaaggtg gaaaatctta cgaattttta cgaaaagtat cgtgtctatc
     5941 tgactcgtcc acgtttagag cttttggcag tagttaccat tgttttctgt gctgtactcg
     6001 tcttttttct aaatattcca ggaaaaggtg tcttaaaact cgataatgga acgattgttt
     6061 atgatggcag tcttgtccgt ggtaaaatga atggtcaagg taccattacc ttccaaaatg
     6121 gagaccaata tacaggtggc ttcaacaatg gagccttcaa cggaaaaggt acctttcaat
     6181 ctaaagaagg ctggacctac gaaggtgatt ttgtaaatgg tcaggctgaa ggaaaaggga
     6241 aactaacaac agaacaagaa gtcgtttatg aaggaacttt taaacaaggc gtttttcaac
     6301 aaaaataaag cctccttatc caaaggaggt attattagaa ccacaaggta agcgcttacc
     6361 tgtaaatccc tttctttcca aatccctctt ccaagcaagt ttgtgaaata aaaaa
//
LOCUS       DAVGJL010000106         6216 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.107, whole genome shotgun sequence.
ACCESSION   DAVGJL010000106 DAVGJL010000000
VERSION     DAVGJL010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6216
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.107"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..5859
                     /locus_tag="VVE42_001710"
     CDS             <1..5859
                     /locus_tag="VVE42_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU3073869.1"
                     /translation="EKYFGEKQERFSFRKLSVGLVSATISSLFFMSVLASSSVDAQET
                     AGVHYKYVADSELSSEEKKQLVYDIPTYVENDDETYYLVYKLNSQNQLAELPNTGSKN
                     ERQALVAGSSLAALGILIFAVSKKKVKNKTVLHLVLVAGIGNGVLVSVHALENHLLLN
                     YNTDYELTSGEKLPLPKEISGYTYIGYIKEGKTTSDFEVSNQEKSAATPTKQQKVDYN
                     VTPNFVDHPSTVQAIQEQTPVSSTKPTEVQVVEKPFSTELINPRKEEKQSSDSQEQLA
                     EHKNLETKKEEKISPKEKTGVNTLNPQDEVLSGQLNKPELLYREETIETKIDFQEEIQ
                     ENPDLAEGTVRVKQEGKLGKKVEIVRIFSVNKEEVSREIVSTSTTAPSPRIVEKGTKK
                     TQVIKEQPETGVEHKDVQSGAIVEPAIQPELPEAVVSDKGVPEVQPALSEAVVTDKGE
                     PAVQPELPEAVVSDKGEPAVQPELPEAVVTDKGETEVQPESPDTVVSDKGEPKQVAPL
                     PEYKGNIEQVKPETPVEKTKEQGPEKTEEVPVKPTEETPVNPNEGTTEGTSIQGAENP
                     VQPAEESTTNSEKVSPDTSSENTGEVSNKPSDSKPPVEESNQPENSGNTTSENGQTEP
                     EPSNGNSTENVSTKSNTSNSNGNEEIKQENELDPDKKVEDPEKTLELRNVSDLELYSL
                     SNGTYKQHISLEQVPSNPNSYFVKVKSSSFKDVYLPVASISEERKNDKILYKITAKVE
                     KLQQEIESRYKDNFTFYLAKKGTEETTNFTSFSNLVKAINQNLSGTYHLGASLNANEV
                     ELSTDDKSYIKGTFTGQLIGEKDGKHYAIYNLKKPLFESLRGATIEKLSLKNVSISGK
                     DDIGSLANEAQNNTKIKQVHVDGVLAGERGIGGLLAKADQSSITESSFKGRIVNTYET
                     TASYNIGGLVGHLTGSKASLTKSKATVVISSNTNSSDQTVGGIAGLVDKDAHIQNSYS
                     EGDINNSQRFGKVAGIAGNLWDRESNSENHAGRLTNVLSDVNVTNGNAISGYHYNGMK
                     ITDAFSNKANKVFNVTLEKDEVVSKESFEERGTMLDASQIASKKAEINLLTPPIVKPL
                     STSGKKDSDFSKIAHYQANRALVYKNIEKLLPFYNKATIVKYGNLVKENSILYQKELL
                     SAVMMKDDQVITDIISNKQTANKLLLHYKDHSSEKFDLRYQADFAKLAEYSLGDTGLL
                     YTPNQFLYDQDSIINQVLPELQQVAYDSEAIRKTLGISPEVKQTELYMEEQFTKTKQD
                     LANSLKKLLSADAGLAGDNPVTRGYLVDKIKNNKEALLLGLTYLERWYNFSYGQVNVK
                     DLVMYHPDFFGKGNTSPLDTLIELGKSGFNNLLAKNNVDTYAISLASHHGTTDLFSTL
                     ENYRKVFLPDKTNNDWFKSQTKAYIVEEKSNIEEVKTKQGLVGTKYSIGVYDRITSDS
                     WKYRNMVLPLLTLPERSVFVISTISSLGFGAYDRYRNKEHQANGDLNSFVEKSAHETA
                     ERQRDHYDYWYRILDEKGREKLYRNILLYDAYKFGTDHTEGKATEVANFDNPNPAMKH
                     FFGPVGNKVGHNGHGAYATGDAVYYMGYRMLDKDGAITYTHEMTHDSDQDIYLGGYGR
                     RSGLGPEFFAKGLLQAPDQPSDATITINSILKHKTSDSTEGQRLQVLDPTTRFNDAAD
                     LQNYVHNMFDVVYMLEYLEGQSIVKQLDAYQKMTALRKIENKYVKDPADGNDVYATNV
                     VKNLTEDEAKKLTSFDSLIDNNILSAREYKAGTYERNGYFTIKLFAPIFSALSSEKGT
                     PGDLMGRRIAYELLAAKGFKDGMVPYISNQYEEDAKQQGQTINLYGKERGLVTDELVL
                     KKVFDGKYKTWAEFKTAMYQERVNQFGNLKQVTFKDPTKRWPSYGTKTINNVDELQKL
                     MDQAVLQDATGTRWSNYNPETDSAVHKLKRAIFKAYLDQTNDFRSSIFENKK"
BASE COUNT         2241 a         1074 c         1258 g         1643 t
ORIGIN      
        1 gaaaagtatt ttggtgaaaa acaagagcgt ttttcattta gaaaattatc agtaggactt
       61 gtatctgcaa cgatttcaag tttatttttt atgtctgtat tagctagttc atctgtggat
      121 gctcaagaaa ctgcgggagt tcactataaa tatgtggcag attcagagct atcatcagaa
      181 gaaaagaagc agcttgtcta tgatattccg acatacgtgg agaatgatga tgaaacttat
      241 tatcttgttt ataagttaaa ttctcaaaat caactggcgg aattaccaaa tactggaagc
      301 aagaatgaga ggcaagccct agttgctggt tctagcttag ctgctctggg aattttaatt
      361 tttgctgttt ccaagaaaaa ggttaagaat aaaacggtat tacatttagt attggttgcg
      421 ggaataggaa atggtgtctt agtttcagtc catgctttag aaaatcatct tttgctaaat
      481 tacaatacgg actatgaatt gacctctgga gaaaaattac ctcttcctaa agagatttca
      541 ggttacactt atattggata tatcaaagag ggaaaaacga cttctgattt tgaagtaagt
      601 aatcaagaaa aatcagcagc cactcctaca aaacaacaaa aggtggatta taatgttaca
      661 ccaaattttg tagaccatcc atcaacagta caagctattc aggaacaaac acctgtttct
      721 tcaactaagc cgacagaagt tcaagtagtt gaaaaacctt tctctactga attaatcaat
      781 ccaagaaaag aagagaaaca atcttcagat tctcaagaac aattagccga acataagaat
      841 ctagaaacga agaaagagga gaagatttct ccaaaagaaa agactggggt aaatacatta
      901 aatccacagg atgaagtttt atcaggtcaa ttgaacaaac ctgaactctt atatcgtgag
      961 gaaactatag agacaaaaat agattttcaa gaagaaattc aagaaaatcc tgatttagct
     1021 gaaggaactg taagagtaaa acaagaaggt aaattaggta agaaagttga aatcgtcaga
     1081 atattctctg taaacaagga agaagtttcg cgagaaattg tttcaacttc aacgactgcg
     1141 cctagtccaa gaatagtcga aaaaggtact aaaaaaactc aagttataaa ggaacaacct
     1201 gagactggtg tagaacataa ggacgtacag tctggagcta ttgttgaacc cgcaattcag
     1261 cctgagttgc ccgaagcagt agtaagtgat aaaggcgtac cagaagttca accagcgtta
     1321 tcggaagcag tcgtaaccga caaaggtgag cccgctgtcc aaccagagct accggaagca
     1381 gtagtaagtg ataaaggtga accagcagtc caaccagagc taccggaagc ggttgtgact
     1441 gacaaaggtg agactgaggt tcaaccagag tcgccagata ctgtggtaag tgataaaggt
     1501 gaacctaaac aggtagcacc gcttccagaa tataagggta atattgagca agtaaaacct
     1561 gaaactccgg ttgagaagac caaagaacaa ggtccagaaa aaactgaaga agttccagta
     1621 aaaccaacag aagaaacacc agtaaatcca aatgaaggta ctacagaagg aacctcaatt
     1681 caaggagcag aaaatccagt tcaacctgca gaagaatcaa caacgaattc agagaaagta
     1741 tcaccagata catctagcga aaatactggg gaagtatcca ataaacctag tgattcaaaa
     1801 ccaccagttg aagaatcaaa tcaaccagaa aattcaggta atacaacatc agagaatgga
     1861 caaacagaac cagaaccatc aaacggaaat tcaactgaga atgtttcaac caaatcaaac
     1921 acatccaatt caaatggaaa cgaagaaatt aaacaagaaa atgaactaga ccccgataaa
     1981 aaggtagaag atccagagaa aacacttgaa ttaagaaatg tttccgacct agagttatac
     2041 agtttgtcaa atggtactta taaacaacac atttcgttag aacaagttcc aagcaatcca
     2101 aatagctact ttgttaaagt gaaatcttct tcattcaaag atgtatacct accagtagca
     2161 tcaatatcag aggaaagaaa aaatgataaa atcctttata aaatcacagc aaaagtagag
     2221 aagcttcagc aggagataga aagcagatat aaagataatt ttaccttcta tctagctaag
     2281 aagggaacag aagaaacaac aaactttact tcctttagta atctggtcaa agctataaac
     2341 caaaatctct ctggaaccta tcatttagga gctagcctga atgctaacga agtggagctt
     2401 agtactgatg ataaatccta tatcaaggga acctttactg gtcagttgat tggtgaaaaa
     2461 gatggtaagc attatgctat ttacaacttg aaaaagcctc tttttgaaag cttgagaggg
     2521 gccacaatag aaaagttgag tctgaaaaat gtctctattt cagggaaaga tgatatcggt
     2581 tcactggcaa atgaagctca gaataacaca aaaattaagc aagttcatgt ggatggtgta
     2641 cttgccggtg aacgtggtat cggtggtttg ttggctaagg cagatcaatc aagcatcaca
     2701 gagagtagtt tcaagggaag aattgtcaat acctatgaaa caactgcttc ctataatatc
     2761 ggtggcctgg tcggtcattt gacaggaagc aaagcttcac tgactaagtc aaaagcgaca
     2821 gtggtcattt catcaaatac aaatagttct gaccagaccg ttggaggtat cgctggtctt
     2881 gtagacaaag atgcgcatat tcagaatagc tattctgaag gggatattaa taatagtcaa
     2941 cgttttggaa aagttgctgg tatagcaggt aacttatggg atcgtgaatc aaatagtgaa
     3001 aatcatgctg gaagattgac taatgtgctt agtgatgtca atgtaactaa tggaaatgcc
     3061 attagtggtt accattataa tggaatgaaa attactgatg catttagtaa taaagcgaat
     3121 aaagttttca atgtcacttt agagaaggat gaggtcgtca gcaaagaatc ctttgaggaa
     3181 agaggaacaa tgctagatgc ttctcaaatt gcaagcaaaa aagcagaaat caatcttctc
     3241 actccaccaa tagtgaaacc cctctcaaca agcggtaaga aagacagtga tttttctaag
     3301 atagcccatt atcaagccaa ccgtgctttg gtttataaga acattgaaaa attgttacct
     3361 ttctataaca aggcgaccat cgtgaaatac ggaaacctgg ttaaggagaa cagtatctta
     3421 tatcaaaaag aactcttgtc tgcagttatg atgaaggatg accaagtaat cacagatatt
     3481 atttccaaca aacagactgc aaacaaactc ttgcttcact acaaggacca ttcatctgaa
     3541 aagtttgatc tcaggtacca ggctgatttt gctaagttag cagaatatag tctaggagat
     3601 actggtcttc tctatacgcc aaaccaattc ttgtatgacc aagactctat catcaatcaa
     3661 gttttacctg aattacagca ggttgcctac gattcagaag ctatcagaaa gacactcgga
     3721 atttctccag aggtcaagca aactgagctc tatatggaag aacaatttac caaaacaaag
     3781 caagatttgg caaacagttt gaaaaaactc ttgtctgcag atgctggact tgctggtgac
     3841 aacccagtta ccagaggcta tcttgtagat aaaatcaaga acaataagga agccttacta
     3901 ctcggtttaa cttatttaga acgttggtat aactttagct atggtcaagt gaatgtcaaa
     3961 gacctagtta tgtatcatcc ggacttcttt ggtaaaggaa atacttcacc attagatact
     4021 ctgattgagt taggtaaatc tggctttaac aatcttcttg ctaaaaacaa tgtcgatact
     4081 tatgctatca gtcttgccag ccatcatgga acgacagatt tgtttagcac gttggaaaat
     4141 taccgaaaag tctttctacc agacaaaacc aataatgact ggtttaaatc acagactaag
     4201 gcttacattg tcgaagaaaa atccaatatc gaagaggtga aaacgaagca aggactagtt
     4261 ggtactaagt attctattgg tgtctatgat cgaatcacta gcgacagctg gaaataccgt
     4321 aatatggtac tacctctact taccttgcca gaaagatccg tatttgtcat ttcaaccata
     4381 tctagtctag gttttggtgc ttatgatcgc tatcgtaata aagaacatca agcaaatggt
     4441 gatcttaata gctttgttga aaagagtgct cacgaaacag cagagcgtca acgagatcac
     4501 tatgattatt ggtatcgtat tctagatgaa aaagggcgtg aaaagcttta tcgaaatatc
     4561 ttgctttatg atgcctataa atttggaact gatcatactg aaggaaaggc tacagaagta
     4621 gcaaattttg ataatccaaa tcctgcaatg aaacatttct ttggacctgt tggaaataag
     4681 gttggacata atggacatgg tgcttatgct acaggggatg ccgtttatta tatgggttat
     4741 cgtatgttgg acaaggacgg cgccattact tatacgcatg agatgacaca tgactcagat
     4801 caggacattt atcttggagg ttatggaaga agaagtggac ttggacctga gttctttgct
     4861 aaaggcttac tacaagcacc agaccaacca agtgatgcaa ccattaccat caactctatc
     4921 ttgaaacata aaacatcaga tagtacagaa ggtcagcgat tgcaagtact tgatccaact
     4981 acaagattta atgacgcagc agatcttcag aactacgtcc acaatatgtt tgatgtcgtt
     5041 tacatgttgg aatatctcga agggcaatct atcgtgaaac agttagatgc ttatcagaaa
     5101 atgacggccc tgagaaaaat cgagaataaa tacgtgaaag atcctgcaga tggaaatgat
     5161 gtttatgcta ctaatgttgt gaaaaatttg actgaagatg aagctaaaaa attgacgagc
     5221 tttgatagtt tgattgacaa taacattctt tcggctcgtg agtataaggc tggcacttat
     5281 gagagaaatg gctactttac tataaaactc tttgcaccaa tcttttcagc attgagcagt
     5341 gagaaaggaa ctccaggcga ccttatggga cgtcgtatag cctatgaact actagctgct
     5401 aaaggcttta aagatggtat ggtaccatat atctcaaatc aatacgaaga agatgctaaa
     5461 caacaagggc aaactatcaa tctttatggt aaagaacggg gcttggtgac cgatgagctt
     5521 gttttgaaaa aggtatttga tggtaagtat aaaacttggg cagaatttaa gacagctatg
     5581 taccaagaac gcgtgaatca gtttggaaac ttgaagcagg tgacctttaa agatccgaca
     5641 aaacgatggc caagctatgg cacaaagact atcaataatg tggatgaatt gcagaaacta
     5701 atggatcaag ctgttttgca ggatgctaca ggtacacgtt ggagtaatta taatccagaa
     5761 acggacagtg ccgtccataa gttgaagaga gcaatcttta aagcctatct tgaccaaaca
     5821 aatgatttta gaagttcaat ttttgagaat aaaaaatagt gtttactata tagtgtttac
     5881 tattaggaaa taaagtttaa aaaggtgatg aagaacaaac caagattcaa gcaggaattc
     5941 ctactgataa tgaagtaagt tatgctctta tttatcagca ggaaactctt cctgcaacag
     6001 gttcatcttt atcagcagga aactcttcct gcaacaggtt catcaacttc tgcacttaca
     6061 gctttaggcg cctattagct gttggtagtt tagttctttt ggttcataat atgacgggaa
     6121 cagttttttg ctccctctga aaagtcatca tttgatggct tttttctata tagggtaaaa
     6181 gatagggtaa aaggctatca tcggacaaaa taaaga
//
LOCUS       DAVGJL010000107         6208 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.108, whole genome shotgun sequence.
ACCESSION   DAVGJL010000107 DAVGJL010000000
VERSION     DAVGJL010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6208
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.108"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            86..1567
                     /locus_tag="VVE42_001711"
     CDS             86..1567
                     /locus_tag="VVE42_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU3073870.1"
                     /translation="MRDLLSKKSHRQLELLELLFEHKRWFHRSELAELLNCTERAVKD
                     DLSHVKSAFPDLIFHSSTNGIRIINTDDSDIEMVYHHFFKHSTHFSILEFIFFNEGCQ
                     AESICKEFYISSSSLYRIISQINKVIKRQFQFEVSLTPVQIIGNERDIRYFFAQYFSE
                     KYYFLEWPFENFSSEPLSQLLELVYKETSFPMNLSTHRMLKLLLVTNLYRIKFGHFME
                     VDKDSFNDQSLDFLMQAEGIEGVAQSFESEYNISLDEEVVCQLFVSYFQKMFFIDESL
                     FMKCVKKDSYVEKSYHLLSDFIDQISVKYQIEMENKDNLIWHLHNTAHLYRQELFTEF
                     ILFDQKGNTIRNFQNIFPKFVSDIKKELSHYLETLEVCSSSMMVNHLSYTFITHTKHL
                     VINLLQNQPKLKVLVMSNFDQYHAKFVAETLSYYCSNNFELEVWTELELSKESLEDSS
                     YDIIISNFIIPPIENKRLIYSNNINTVSLIYLLNAMMFIRLDE"
     gene            1929..2924
                     /locus_tag="VVE42_001712"
     CDS             1929..2924
                     /locus_tag="VVE42_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU3073871.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLRKVQR
                     AIKTLDYIPDNYARGMKKNRTETIALIIPSIWHPFFSEFAYHVEKELLKYNYKLFICN
                     ADSDSQNEIEYIEMLKQNKVDGIIGITYSDIDKYILSDLPFVSIDRHFSENVCYVTSA
                     NYKGGQTAAEELVRRDNKHLAFIGSINMYENETILRSKGFREKAKELGVDVYNFEMKE
                     PLINPEDQIRIFLQSHKEIDGIFTINDMMALRVISVMKELGKIAPDDYQIIGFDGLKT
                     SVDQSYLVSTIVQDIGMMAKESVAMILKMIKREEILEKRIVIPTYFWGDKTTKKI"
     gene            3051..4361
                     /locus_tag="VVE42_001713"
     CDS             3051..4361
                     /locus_tag="VVE42_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEU3073872.1"
                     /translation="MRKGTMLSVVAGLSLAILAGCSGGTNSKQASSSNDIKVWVQFSD
                     ETNEGKAWQKVVDNFNQSGKSKYKVVTEYIPRSGSGGGYEDKVNAAITTNSLPDVITL
                     DGPNTAAYAKSKVITPLDDYLKDNNMDDVLDSIKQQGTYNGKFYAFGFSESNVGVYYN
                     KKMFKEAGIAESELPTLEKPWTWDEFNTIAKKLKDHYNKPTIDFRINSNDEMLPYAYM
                     PLIWSNNGSVVNEDGTKAEGYFNSKESVEAVQFIQNLVKEGYTTVSPVEKGFETGEYP
                     MLLSGSWTIADMNTNYKDIDFGILPYPVSSKTKKLVSPSGSWQLAVTTKSDKKEAASE
                     FVKFATNTESSEIISLGNSVLPIRKSTIENIKDKVSEPMRFLMEQNSKTAHARPVVVA
                     YPQVSRAFQQAMQDISYYEENPNVQKVLDTRTKEMQTAIDQSLK"
     gene            4457..5359
                     /locus_tag="VVE42_001714"
     CDS             4457..5359
                     /locus_tag="VVE42_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU3073873.1"
                     /translation="MSKETVVDTKRREKIKDNILGYSFLAPALILLGIFLVIPVGMVI
                     YYAFTDYYLLTPDERKFVGLENFIRLTKDPIFLKSFLNTLKFVVWIIPVQLGAALGMA
                     LIVNKKRKGNMFFKVAFFAPVVMSLVVISILWLYLLNPNSGLLNAILNKVGIASQPFL
                     TSPKQAMYAIVFVSAWQGAGYQMLLFLGGMQNIPQDVYEAAELDGFSKWAQFRYITMP
                     LLKPTALFVLLTTLISAFKLIVQPMVMTQGGPLNSTITMVYYIYQQGFTDRLVGYSSS
                     IALVFTTLIGMISLVQRRVLKEDD"
     gene            5361..6206
                     /locus_tag="VVE42_001715"
     CDS             5361..6206
                     /locus_tag="VVE42_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU3073874.1"
                     /translation="MKRFKPTTILEYILLTLLAGLFLFPMVWMVVSAMKPEADVYKNL
                     TSWQAFLPSLNPADWFKPYQEVFERFSIFTYLGNSIFYAGTFAIGSIIVNALAGFAFA
                     KVNFSGKKILFGFLLALLIIPVETVLIPQFTIINSLGLVNNRLAVIIPGLASVFNIYL
                     FRNFFIAIPEEILESAKMDGASIVRIFFRIMLPMSKPAVATVGVLSFISSWNDYIWPL
                     MVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTISTIPLILVYVVAQKYIVQGLGGS
                     GTGIK"
BASE COUNT         2110 a          867 c         1136 g         2095 t
ORIGIN      
        1 agtcgttata tcatgctata cctattcttt gtggtataat tgcaagaggt ttaatccgat
       61 aatttataaa gagagaaaga tattcatgag agatttatta tctaaaaaaa gtcataggca
      121 attagaatta ttagaattat tatttgaaca taaacgttgg tttcatcgtt ctgaactagc
      181 agagttatta aattgtacag aacgtgcagt caaagatgat ctatcccatg ttaaatctgc
      241 ttttcctgac ttgatttttc attcttctac taatggtata cgcattatta ataccgatga
      301 tagtgatatt gaaatggttt accatcattt ctttaaacat tcaactcatt tttcgatttt
      361 agaattcatc ttctttaatg aaggttgtca agctgagagt atttgtaaag aattttatat
      421 cagttcatct tcgctctatc gtattattag ccaaatcaat aaagtgatta aaaggcaatt
      481 tcaatttgaa gtcagtctga cccctgttca aatcattgga aatgagagag acattcgtta
      541 cttttttgca caatattttt cagaaaaata ttatttccta gaatggccat ttgaaaattt
      601 ttcatcagag ccactatctc aattgttaga attggtttat aaggaaacaa gctttccaat
      661 gaatttgtca acgcatagaa tgctaaagtt gctcctagtt acgaacctat atagaataaa
      721 gtttggtcat tttatggaag tagataaaga ttcttttaac gaccaaagtt tggatttttt
      781 aatgcaggca gaaggaatag aaggtgttgc tcagagtttt gaatcagaat acaatatctc
      841 tttagatgaa gaagttgttt gccaattatt tgtgtcttac tttcaaaaaa tgtttttcat
      901 agatgaaagt ctctttatga aatgcgtaaa aaaggatagt tatgttgaaa aatcttacca
      961 tctattgagt gattttattg atcagatttc agtcaagtat cagattgaaa tggagaataa
     1021 ggataatctg atttggcatc tgcataatac cgcacatctg tatcgtcaag agttgtttac
     1081 tgagtttatt ttatttgatc aaaaagggaa tacaatcagg aactttcaaa atatttttcc
     1141 aaaatttgtt tcagatataa aaaaagagct ttctcactat ttagagactc ttgaggtttg
     1201 ttctagttca atgatggtaa atcatctatc ttataccttc atcactcata ccaaacattt
     1261 ggtgattaat ttgctacaaa atcagcctaa actaaaggtt ttggttatga gtaattttga
     1321 tcagtatcat gcaaaattcg ttgcagagac actttcttat tactgtagca ataattttga
     1381 actggaagtt tggactgaat tagaattatc aaaggaatct ttagaagatt catcttatga
     1441 tatcattatt tctaatttta ttattcctcc tattgaaaat aagagactca tctattcaaa
     1501 taatataaac acggtctcac tcatatattt gttaaatgcc atgatgttta ttcgattaga
     1561 tgagtaacca aaaataacaa aaaaacgcta tttcgcgttt gaactgtacc tcaaaagtta
     1621 gacagaaaaa atctaatttt tggggtgttt ttgttaagaa aagaccttca atcatttaag
     1681 caaaatgtac atctagattt atagccctct aaaactcttg ctttttttgt agaagatacg
     1741 aaaaaagcca taaaaccaag gcttttcctg ttttatgtag atgtttacac atgaaaattg
     1801 aagtattttc aaaagctaat tggagtgatt ttattcatga tgggataaat gatctggcca
     1861 aaaatgattg agaaatagtt gcttaaaaag catttgcatg atactatata ttgaaaggag
     1921 gtgataatat ggtagaacaa agaaaatcaa ttaccatgaa agatgttgct ttagaagcag
     1981 gagttagtgt tggaactgtt tcacgtgtaa ttaataaaga aaaaggcatt aaagaagtaa
     2041 ctttaaggaa agttcaaagg gctataaaaa cattggacta tatccctgat aattatgctc
     2101 gaggtatgaa aaagaataga acagaaacta tcgctttgat tattccgagc atatggcatc
     2161 catttttttc ggagtttgcc tatcatgttg agaaagagtt attaaaatat aattataaat
     2221 tatttatttg taacgctgat agcgattcac aaaatgaaat cgaatatatc gaaatgttga
     2281 aacaaaataa agtggatgga attattggta ttacatattc tgatattgac aaatatattt
     2341 tatctgattt accgtttgta agtatagata gacatttttc ggaaaatgta tgctatgtta
     2401 cttcagcaaa ttacaaagga ggacaaactg cagcagagga actggttaga agggataata
     2461 agcaccttgc ttttattggt tcaattaata tgtatgagaa tgagactata ctcagaagta
     2521 aaggattcag agaaaaagct aaggaattag gagtagatgt ctataatttt gaaatgaaag
     2581 aacctttaat aaatcctgaa gatcagataa gaattttttt gcaatctcat aaagaaattg
     2641 atggtatatt tacaataaat gatatgatgg ctctgagagt tattagtgtt atgaaagaac
     2701 tggggaaaat agcacctgat gattaccaga taattgggtt tgatggttta aaaacttctg
     2761 tcgatcaatc ttatctagtg tctacaatag ttcaagatat tggtatgatg gcaaaagaat
     2821 ctgttgcaat gatactgaaa atgattaagc gagaagagat tctagaaaaa cgaatcgtaa
     2881 ttcccacata tttttgggga gataaaacaa caaaaaaaat ttaaaaagcg cttgacaatt
     2941 aatgaaagcg ctgttataat ataatcaaca aatgaaacgt ttcaaaaata ggctagaagc
     3001 ttgtttaaaa aataaataaa aaatgaaacg tttcaaaaag gaggaaaatg atgcgtaaag
     3061 gtacaatgtt atctgttgtt gcggggctat cgttagctat acttgcaggt tgtagtggag
     3121 gaactaactc aaaacaagcg agttcttcaa atgatattaa agtctgggta caattctctg
     3181 acgaaacaaa tgaaggaaaa gcttggcaaa aagttgttga taactttaat caatcaggaa
     3241 aaagtaagta caaggttgta actgagtata ttcctcgtag tggtagtggt ggaggatatg
     3301 aagacaaggt taatgctgct attacgacta atagtcttcc agatgttatt acccttgatg
     3361 gaccaaatac ggcagcttat gcaaaatcta aagtaattac tccgctagat gattatctaa
     3421 aagataataa tatggatgat gttttggaca gtattaaaca acagggaact tataatggga
     3481 aattctatgc atttggattt tctgaatcga atgttggtgt ttattacaat aaaaaaatgt
     3541 tcaaagaggc cggaattgcg gaatcagagc ttccaacgct agaaaagcca tggacatggg
     3601 acgaatttaa tacgattgct aaaaaattga aagatcatta taacaaacca acaattgatt
     3661 ttcgtattaa ttcaaacgat gaaatgttgc catatgctta tatgccgttg atttggtcaa
     3721 ataatggttc tgtcgtaaac gaagatggaa caaaggcaga aggatacttc aactccaaag
     3781 agagtgtaga agcagttcaa ttcattcaaa atcttgtaaa agaaggctat acaactgtta
     3841 gtccagtcga aaaaggtttt gaaacaggag agtatccaat gcttttgagt ggttcttgga
     3901 cgattgctga catgaataca aactacaaag atattgattt tggtatcctt ccttacccag
     3961 tatctagtaa gacgaaaaaa ttagtatccc cttcaggtag ttggcaatta gctgtgacga
     4021 caaaatctga taaaaaagag gccgcatctg aatttgttaa atttgctaca aatacagaat
     4081 caagtgaaat tattagcttg gggaattcgg tccttccaat tcgtaaatct acaattgaaa
     4141 atatcaaaga taaagtatcc gaaccaatgc gtttcttgat ggaacaaaat tcaaaaacag
     4201 ctcatgctcg cccagttgtt gtagcatatc ctcaggtttc tcgagctttt caacaagcaa
     4261 tgcaagatat tagctattat gaagaaaatc caaatgtgca aaaagtcttg gatactcgta
     4321 caaaagaaat gcaaactgct attgatcagt cactcaaata atctgtaaaa aagaaggtgg
     4381 aaggaaaatg tttggaacta ccttcccctt tcttgttaat agcctagttt gatatatatg
     4441 attaaaggag atggatatga gtaaagaaac tgttgtggat acaaaaagac gtgaaaaaat
     4501 aaaggataac atattaggtt atagtttttt agctccagct ttaattttat taggtatctt
     4561 tttagtaatt ccagttggaa tggtcattta ttacgctttt acagattatt atttgttgac
     4621 accagatgaa agaaaatttg ttggacttga aaattttatt cgattaacaa aagatccgat
     4681 tttcttaaag agcttcttga atactttgaa atttgttgtt tggattattc cagttcaatt
     4741 gggagcggcc ttaggaatgg ctttgattgt caataaaaag cgaaaaggaa atatgttttt
     4801 taaagtggct ttttttgctc cagttgtcat gtccttggta gttatttcta ttctttggct
     4861 ttatctttta aatccaaata gtggcttatt aaatgctatt ttaaataagg taggtattgc
     4921 ttctcaacct ttcttgacta gtcctaaaca agctatgtat gcaattgttt ttgtttctgc
     4981 ttggcagggt gcaggttatc agatgctttt atttttagga ggaatgcaga atattccaca
     5041 agatgtctat gaagctgctg aattagatgg attctcaaaa tgggctcagt ttcgttatat
     5101 cacgatgccc ctactaaaac caacagccct gtttgtattg ttaacaactt taatttctgc
     5161 ttttaagctg attgtacaac caatggttat gacgcaaggt gggccattaa actcaacaat
     5221 tactatggtg tactatattt atcaacaagg ttttactgat cgtcttgtag gatattctag
     5281 ctcaatcgct ttagtattta caacgttaat tggtatgatt agcttagtac aacgtcgcgt
     5341 tctgaaggag gatgactaag atgaaacgat ttaaaccaac aacaatctta gaatatattc
     5401 tgttaacttt attggcagga ctcttccttt ttcctatggt ttggatggta gtttcagcta
     5461 tgaagccaga agcggatgtt tataaaaatt tgactagttg gcaagccttc ttaccaagtt
     5521 taaatcccgc tgattggttt aaaccttatc aggaagtatt tgagcgcttt agtattttta
     5581 catatctagg gaacagcatt ttttatgctg gaacgtttgc tattggttct attattgtca
     5641 atgcactggc cggtttcgct tttgcaaaag tcaatttttc aggtaagaaa attttatttg
     5701 gtttcttact tgctttactt attattccag tggaaactgt gttaattcca caatttacca
     5761 ttatcaattc cttaggtttg gtcaataatc gtcttgcagt tattattcca ggattggcga
     5821 gtgtatttaa tatttactta tttaggaatt tctttattgc aattccagaa gagattcttg
     5881 agtctgcaaa gatggacgga gcaagtatcg ttagaatttt ctttagaatt atgcttccaa
     5941 tgtctaaacc tgcagttgct acagttggtg ttttatcatt tatttcaagc tggaatgatt
     6001 atatctggcc attgatggtt ttgacagatt cttctaaatt ttcaatgcag gttgcaatta
     6061 caactattaa tacaactcaa ccagtttata tcaatcaggt aatggctgta ttaactattt
     6121 caacgattcc gttgattttg gtctatgtag tcgctcagaa atatattgtt caaggtctgg
     6181 gtggttccgg aactggaata aaatagga
//
LOCUS       DAVGJL010000108         6203 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.109, whole genome shotgun sequence.
ACCESSION   DAVGJL010000108 DAVGJL010000000
VERSION     DAVGJL010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6203
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.109"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            34..1734
                     /locus_tag="VVE42_001716"
     CDS             34..1734
                     /locus_tag="VVE42_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEU3073875.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDTLPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVQFHA
                     "
     gene            1727..2203
                     /gene="ilvN"
                     /locus_tag="VVE42_001717"
     CDS             1727..2203
                     /gene="ilvN"
                     /locus_tag="VVE42_001717"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEU3073876.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDEVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            2269..3291
                     /gene="ilvC"
                     /locus_tag="VVE42_001718"
     CDS             2269..3291
                     /gene="ilvC"
                     /locus_tag="VVE42_001718"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEU3073877.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYTVAEATKLADVIMILAPDEIQQELYEAEIAPNLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            3398..3634
                     /locus_tag="VVE42_001719"
     CDS             3398..3634
                     /locus_tag="VVE42_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073878.1"
                     /translation="MLADQNKADWSYDENTVINIYDDANFEDGRLHMTFEQFFKLAQI
                     AREEGLEIHSPFERAGATKSARYIAKWILRNKKH"
     gene            3872..4228
                     /locus_tag="VVE42_001720"
     CDS             3872..4228
                     /locus_tag="VVE42_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEU3073879.1"
                     /translation="MNITNLFSIKTGCDETDRQLQKLFFQLDLQLGELTDQLRKLDSN
                     FVPRSQFVDTLDLNDVEYKEILNYFIFHRNDSEESLVEWLYDWISTNRYELPKEFSIR
                     MAHKYHESVTEVFGDE"
     gene            4278..5528
                     /gene="ilvA"
                     /locus_tag="VVE42_001721"
     CDS             4278..5528
                     /gene="ilvA"
                     /locus_tag="VVE42_001721"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HEU3073880.1"
                     /translation="MLSSKDIIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHIKTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
BASE COUNT         1907 a         1042 c         1419 g         1835 t
ORIGIN      
        1 caagttattt ttaagaggaa agaaagggga acaatggaga aaatcagttt agaatctcct
       61 aagacggggt cggacctagt tttggaaaca cttcgtgatt taggagttga taccatcttt
      121 ggttatcctg gtggtgcggt tttgcctttt tatgatgcga tatataattt taaaggcatt
      181 cgccacattc tagggcgcca tgagcaaggt tgtttgcatg aagctgaagg ttatgccaaa
      241 tcaactggaa agttgggtgt tgccgtcgtc actagtggac caggagcaac aaatgccatt
      301 acagggattg cggatgccat gagcgatagc gttccccttt tggtctttac aggtcaggtg
      361 gcgcgagcag ggattgggaa ggatgccttt caggaggcag acatcgtggg aattaccatg
      421 ccaatcacta agtacaatta ccaagttcgt gagacagctg atattccgcg tatcattacg
      481 gaagctgtcc atatcgcaac tacaggccgt ccagggccag ttgtaattga cctaccaaaa
      541 gacatatctg ctttagaaac agacttcatt tattcaccag aagtgaattt accaagttat
      601 cagccgactc ttgagccgaa tgatatgcaa atcaagaaaa tcttgaagca attgtccaag
      661 gctaaaaagc cagtcttgtt agctggtggt ggaattagtt atgctgaggc tgctacggaa
      721 ctaaatgaat ttgcagaacg ctatcaaatt ccagtggtaa ccagtctttt gggacaagga
      781 acgattgcaa cgagtcaccc actctttctt ggaatgggag gcatgcacgg gtcattcgca
      841 gcaaatattg ccatgacgga agcggacttt atgattagta ttggttctcg tttcgatgac
      901 cgtttgacgg ggaatcctaa gactttcgct aagaatgcta aggttgccca cattgatatt
      961 gacccagctg agattggcaa gattatcagt gcagacattc ctgtagttgg agatgctaag
     1021 aaggccttgc aaatgttgct agcagaacca acagttcaca acaatactga aaagtggatt
     1081 gagaaagtca ctaaagacaa gaatcgtgtt cgttcttatg acaagaaaga gcgtgtggtt
     1141 caaccgcaag cagttattga acgaattggt gaattgacga atggagatgc cattgtggta
     1201 acagacgttg gtcaacacca aatgtggaca gctcagtatt atccctacca aaatgaacgt
     1261 cagttagtga cttcaggtgg tttgggaaca atgggctttg gaattccagc agcaatcggt
     1321 gctaaaattg ctaacccaga taaggaagta gtcttgtttg ttggggatgg tggtttccaa
     1381 atgaccaacc aggagttggc tattttgaat atttacaagg tgccaatcaa ggtggttatg
     1441 ctgaacaatc attcactcgg aatggttcgc cagtggcagg aatccttcta tgaaggcaga
     1501 acatcagagt cggtctttga tacccttcct gatttccaat tgatggcgca ggcttatggt
     1561 attaaaaact ataagtttga caatcctgag accttggctc aagaccttga agtcatcact
     1621 gaggatgttc ctatgctaat tgaggtagat atttctcgta aggaacaggt gttaccaatg
     1681 gtaccggctg gtaagagtaa tcatgagatg ttgggggtgc agttccatgc gtagaatgtt
     1741 aacagcaaaa ctacaaaatc gttcaggagt cctcaatcgc tttacaggtg tcctatctcg
     1801 tcgtcaggtt aatattgaaa gcatctctgt tggagcaaca gaagatccga atgtatcgcg
     1861 tatcactatt attattgatg ttgcttctca tgatgaagtg gagcaaatca tcaaacagct
     1921 caatcgtcag attgatgtga ttcgcattcg agatattaca gacaagcctc atttggagcg
     1981 cgaggtgatt ttggttaaga tgtcagcgcc agctgagaag agagctgaga ttttagcgat
     2041 tattcaacct ttccgtgcaa cagtggtaga cgtagcgcca agctcgatta ccattcagat
     2101 gacgggaaat gcagaaaaga gcgaagccct attgcgagtc attcgcccat acggtattcg
     2161 caatattgct cgaacgggtg caactggatt tacccgcgat taaaaatcca acttaaattt
     2221 attaaaccag cctaaaaggc aataaataat agaaaagaga gaaaagctat gacagttcaa
     2281 atggaatatg aaaaagatgt taaagtagca gcacttgacg gtaaaaaaat cgccgttatc
     2341 ggttatggtt cacaagggca tgcgcatgct caaaacttgc gtgattcagg tcgtgacgtt
     2401 attataggtg tacgtccagg taaatctttt gataaagcaa aagaagatgg atttgatact
     2461 tacacagtag cagaagctac taagttggct gatgttatca tgatcttggc gccagacgaa
     2521 attcaacaag aattgtacga agcagaaatc gctccaaact tggaagctgg aaacgcagtt
     2581 ggatttgccc atggtttcaa catccacttt gaatttatca aagttcctgc ggatgtagat
     2641 gtcttcatgt gtgctcctaa aggaccagga cacttggtac gtcgtactta cgaagaagga
     2701 tttggtgttc cagctcttta tgcagtatac caagatgcaa caggaaatgc taaaaacatt
     2761 gctatggact ggtgtaaagg tgttggagcg gctcgtgtag gtcttcttga aacaacttac
     2821 aaagaagaaa ctgaagaaga tttgtttggt gaacaagctg tactttgtgg tggtttgact
     2881 gcccttatcg aagcaggttt cgaagtcttg acagaagcag gttacgctcc agaattggct
     2941 tactttgaag ttcttcacga aatgaaattg atcgttgact tgatctacga aggtggattc
     3001 aagaaaatgc gtcaatctat ttcaaacact gctgaatacg gtgactatgt atcaggtcca
     3061 cgtgtaatca ctgaacaagt taaagaaaat atgaaggctg tcttggcaga catccaaaat
     3121 ggtaaatttg caaatgactt tgtaaatgac tataaagctg gacgtccaaa attgactgct
     3181 taccgtgaac aagcagctaa ccttgaaatt gaaaaagttg gtgcagaatt gcgtaaagca
     3241 atgccattcg ttggtaaaaa cgacgatgat gcattcaaaa tctataacta attagaaata
     3301 tatagcgctg gagatgattt tatgaaaaag attatgagaa aaattgcatc gttattattg
     3361 gttctagttg tataatgtaa ttacaccgtc ggtaatagtg ctagcagacc aaaataaagc
     3421 agattggtcg tatgatgaaa atactgtaat taacatttat gatgatgcta attttgaaga
     3481 tggtaggttg catatgacct ttgaacaatt cttcaaattg gcacaaatag ctagagaaga
     3541 aggtcttgaa attcattctc cgtttgagag agctggtgcg actaaatctg ctcgttatat
     3601 agcgaaatgg attttgagaa ataaaaaaca ttaacaaata tagttggtaa atcattagga
     3661 cctaaatcag ctgttagatt cggagaagct ttatcctata ttgaaggtcc tcttcgcaga
     3721 ataaatgaga cgatagatgg cggtttatat caaatagagc aaattattgc atctggattg
     3781 aaagaatcgg gtttaaatga ctggactgcg aaaactttag cttcagctat tcgtgggata
     3841 ttagatgtac ttatttaggg gttgaaatca tatgaatatt accaatttgt tttctatcaa
     3901 gacaggatgt gatgaaactg ataggcaact gcaaaaacta ttttttcagt tggatttaca
     3961 attgggagaa ttgacagatc aactaagaaa attagattct aattttgttc ctcgtagtca
     4021 atttgtagac acgttggatt tgaatgatgt agaatataaa gaaattttaa actattttat
     4081 cttccatcgt aatgatagtg aagaaagttt ggtagaatgg ttatatgatt ggatttccac
     4141 aaatcgttat gaacttccta aagagttttc gattcgtatg gctcataaat accatgaaag
     4201 tgttactgaa gttttcggag atgaataact aaaaaacagt cattagtgac tgttttttat
     4261 agaaaaagag gttttatatg ttaagttcaa aagatataat caaggctcac aaggtcttga
     4321 acggtgtggt tgtgaatact ccactggatt acgatcatta tttatcggag aagtatggtg
     4381 ctaagattta tttgaaaaaa gaaaatgccc agcgtgttcg ctcctttaaa attcgtggtg
     4441 cctattatgc catttcccag ctcagcaagg aagaacgtga acgtggggta gtctgcgctt
     4501 ctgcgggaaa tcatgcgcag ggagtagcct atacttgtaa tgaaatgaaa attcctgcta
     4561 ctatctttat gcctattact acgccacaac aaaagattgg tcaggttcgc ttttttggtg
     4621 gggattttgt aactattaaa ctagttggag atacctttga tgcctcagcc aaagcagctc
     4681 aagaatttac agtctctgaa aatcgtacct ttattgatcc ttttgatgat gctcatgttc
     4741 aagcaggtca aggaacagtt gcttatgaga ttttagaaga agctcgaaaa gaatcgattg
     4801 attttgatgc tgtcttggtt cctgttggtg gtggcggtct cattgccggg gtttctacct
     4861 atatcaagga aacaagtcca gagattgagg ttatcggagt agaggcgaat ggagcgcgtt
     4921 ccatgaaagc tgcctttgag gctggaggtc cagtcaaact caaggaaatt gataaatttg
     4981 cggatgggat tgctgtgcaa aaggtaggtc agttgaccta tgaagcaact cgtcaacata
     5041 ttaaaacttt ggtaggtgtc gatgagggat tgatttctga aaccttgatt gacctttact
     5101 ctaagcaagg gatagtcgca gagcctgctg gagcggctag tatcgcctct ttagaggttt
     5161 tagctgaata tattaagggg aaaaccattt gttgtatcat ttctggagga aataatgata
     5221 tcaaccgtat gccagaaatg gaagagcgtg ccttgattta tgatggtatc aaacattact
     5281 ttgtggtcaa tttcccacag cgtccaggag ctttgcgtga gtttgtaaat gatatcctgg
     5341 ggccaaatga tgatatcaca cgttttgagt atatcaaacg agctagcaag ggaacaggcc
     5401 cagtattgat tgggatcgct ttggcagata agcatgatta tgcaggtttg atccgtagaa
     5461 tggaaggttt tgatccagct tatattaact taaatggtaa tgaaacgctt tataatatgc
     5521 ttgtctgagg actaataaaa aaatatcata ttatttgtac aactgatgta tatgtgctat
     5581 aatgaagatg aagaaagggg acgattccta tggttttaga aaacttatcg tgcaattctt
     5641 acttctgttc attatagcac ttatgatgtt tggctcagat ttccatcctt cattttgatt
     5701 tcctatctat tgacaagcat agtcacactg tctttaatac tcttcgaaaa tctcttcaaa
     5761 ccacgttagc tctatctgca acctcaaaac agtgttttga gcaacttgcg gctagcttcc
     5821 tagtttgctc tttgattttc attgagtata aggtatgatt tgatttcttt ttgttgacaa
     5881 atataccata ttaaaaggat atataagtaa ttaactgagc ttatctgtct tgtcatcttt
     5941 attaaggatg gtttagataa tcgggtgtct gcttctaggc tagcacctca atatccaaag
     6001 gagtgatgaa tttgaaggat ataaggaata cctatctctc agatgattta ttgaggaaga
     6061 aagataggag tttttgagct agtgaaggct tggatttcta aaggttagaa ctatcatctt
     6121 cagttcttaa atcgaagaaa taagctatct tacggaaata gagaagcatt tttaagaact
     6181 tgaataattt cgcaccttaa gag
//
LOCUS       DAVGJL010000109         6113 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.110, whole genome shotgun sequence.
ACCESSION   DAVGJL010000109 DAVGJL010000000
VERSION     DAVGJL010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6113
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.110"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..416
                     /locus_tag="VVE42_001722"
     CDS             <1..416
                     /locus_tag="VVE42_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEU3073881.1"
                     /translation="KALNTLSNFYSLVGREQEYEANYSHLIELYQTKNLDHQEFLFGY
                     IRVRYNYAHYLVSKEKYNEAIQEALETIELCKQRQTSYQLAPLLILVGNAGAKFLDRE
                     QVKNYYIEARELCKIYNNPLMLMKIENYLKELDTV"
     gene            799..1419
                     /locus_tag="VVE42_001723"
     CDS             799..1419
                     /locus_tag="VVE42_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073882.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTAFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYIGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIVTLIFF"
     gene            1505..1948
                     /gene="tsaE"
                     /locus_tag="VVE42_001724"
     CDS             1505..1948
                     /gene="tsaE"
                     /locus_tag="VVE42_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEU3073883.1"
                     /translation="MYTKNEEELQALGERLGHLLAKNDVLILTGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLLEELQYGV"
     gene            1938..2456
                     /locus_tag="VVE42_001725"
     CDS             1938..2456
                     /locus_tag="VVE42_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU3073884.1"
                     /translation="MEYELLIREAEPKDAAELVAFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVRHIGDLFIVIGKRYWNNG
                     LGSLLLEEAIEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLIG"
     gene            2464..3480
                     /locus_tag="VVE42_001726"
     CDS             2464..3480
                     /locus_tag="VVE42_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEU3073885.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNVERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEEALVYARMRYQDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSVGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRAHMLEMQNILRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            3565..4821
                     /locus_tag="VVE42_001727"
     CDS             3565..4821
                     /locus_tag="VVE42_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEU3073886.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FALRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNQLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQIDPTLAPYAKTGEVTLR
                     LSTKASSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGVSSIFEGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQDQGTEVIKVNIG
                     GRSRADVRHIAVMHAFNLVRKALLSD"
     gene            4876..6042
                     /gene="recA"
                     /locus_tag="VVE42_001728"
     CDS             4876..6042
                     /gene="recA"
                     /locus_tag="VVE42_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEU3073887.1"
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVTLDLGDELEIEIEE"
BASE COUNT         1915 a          999 c         1418 g         1781 t
ORIGIN      
        1 taaaggcgtt gaatactcta tctaatttct attccttagt gggtcgtgaa caagaatatg
       61 aggcaaacta ctctcattta atagagttgt atcagacaaa aaatttggac catcaagagt
      121 ttttatttgg ctacatcaga gttcgttaca actacgctca ctacctagta tcaaaggaaa
      181 aatataatga agccatccaa gaagctcttg aaacgattga actctgtaaa caaagacaga
      241 caagctacca attggctcct ctacttattc ttgtaggaaa tgctggagcc aaatttctag
      301 acagagaaca agtcaaaaat tattatatag aagcaagaga gttatgtaag atttataaca
      361 atcctttaat gttgatgaag atagaaaatt atttgaagga actagatact gtttagttaa
      421 tcttgacatt atagtttttc tgaaacgtta aataacctgt aaggctgata gttagattaa
      481 tactatcagc cttataatta tgtgactctg agtccgtctc gctccgtgag gtgcgagaca
      541 aaacaaccac ccgtttgacg gattttcatg atttcaaatt tatattcttc gtaaatctct
      601 tcaaaccacg tcagctctat ctgcaatctc aaaacagtgt tttgagcaac ctgtggctag
      661 ctacctagtt tgctctttga ttttcattga gtattagtct ctagtgtttg tttagcgtct
      721 taaagagaca tttgagaaga ttagaaacag ttctctcttt tctcctagta taatgattgg
      781 taggaaggga gagagaagat gaaatcaatg agaatcttat ttttgttagc tttaattcaa
      841 atcagtttga gtagctgttt cctatggaag gaatgcatct tgtcctttaa acaaagtaca
      901 gcttttttca tcggaagcat ggttttcgtt tcaggaatct gtgctggagt aaattatctt
      961 tatactcgta agcaagaagt ccatagtgtc ctagccagta agaagtcggt gaagcttttt
     1021 tacagtatgt tactcttaat taatttgtta ggagctgttc ttgttttgtc agataacttg
     1081 ttcatcaaaa atacgctgca gcaagaatta gttgactttt tattgccatc cttctttttc
     1141 ctatttgggc tagatttgct gattttttta cccttgaaaa aatacgtgcg cgattttctt
     1201 gctatgctgg acagaaaaaa gacagtgttg gtgactattt tagcaacact tcttttctta
     1261 agaaatccaa tgaccattgt ctcacttctg atttatattg gactgggctt gttttttgca
     1321 gcctatcttg tcccaaattc ggttaagaag gaagtttcct tttatggtca tattttccga
     1381 gatcttgtat tggtcattgt tacgctcatt ttcttttaga gaaagcctgt tttttatgga
     1441 tgttatgatg ataagtttag taggaaaata gacacaaaag aaaagttttt ggtataataa
     1501 gagtatgtac acaaaaaatg aagaagagtt gcaagcctta ggggagcgtt tgggccatct
     1561 attagcaaag aatgatgttt taatcttaac tggagaactg ggtgcaggta aaacgacctt
     1621 tactaaagga cttgcaaaag gattacagat ttctcaaatg attaaaagtc ccacctatac
     1681 tatcgtgaga gagtatgaag gtcgacttcc actttatcac ctagatgttt atcgtattga
     1741 aggagatgct gattctatcg acttggatga gtttatcttt ggtggcggcg tgactgttat
     1801 tgagtgggga aatctcttag gagatgcctt gccagatgct tatttagaat tggaaattct
     1861 aaaagaagca gatggacgcc gtttaaattt tcaggcaaag ggtttgcgtg ctgagaaatt
     1921 gttagaggag cttcaatatg gagtatgaat tgctcattag ggaagcagag cccaaagatg
     1981 cagctgaatt agtggccttt ttaaatcgtg tgagtttgga gacagacttt accagcctag
     2041 acggagatgg tattctcttg actagtgagg agatggaaat attcctcaac aagcaagcta
     2101 gttcggacaa tcagataacc ttacttgcat tcttaaatgg caaaattgct ggtattgtaa
     2161 atattacagc tgatcagcgt aagagagtcc gtcatattgg agatctcttc attgtgattg
     2221 gaaaaagata ttggaataat ggcttgggaa gtttgttgct agaagaagcg atagagtggg
     2281 cacaagcaag tggcattctg cgtcgtctcc aactgactgt ccaaactcgt aatcaagcag
     2341 cagtccatct ttatcaaaag catggctttg tcattgaagg tagccaagag cgtggggcat
     2401 atatagaaga agggaaattt atcgatgttt acttgatggg taaactgata ggttagtaga
     2461 aatatggtta aaaaaattat tggaatggtg ctagctttac tttctgtaac tgtagtagga
     2521 gtaggtgttt ttgcttatac tatttatcaa caagggacag aaaccttagc taaaacctat
     2581 aaaaaaatcg gtgaagaaac caaggttatt gaagcgactg aacctctaac cattctgtta
     2641 atgggagtgg acaccggaaa tgttgaacga actgaaactt gggtcggtag aagtgatagc
     2701 atgatcttga tgacagtgaa tcctaaaacg aaaaaaacaa caatgatgag tttagagcgg
     2761 gatattctga cgcgcattga atcagggaat ggtcaggctc atgaagcgaa actgaactca
     2821 gcatatgcag atggtggagc agagcttgct atagaaacca ttcaaaaaat gatgaatatc
     2881 catattgatc gctatgtgat ggtcaatatg agagggttgc aaaaactagt ggatgcagta
     2941 ggaggtatta cagtcaataa tatcctaggt ttcccaattt ctatcagtga ccaagaagaa
     3001 tttaatacta tttctatcgg tgttggggag caacatattg ggggagaaga agccctagtc
     3061 tatgcacgaa tgcgttacca agatcctgag ggggattatg gtcgtcaaaa acgtcaacgt
     3121 gaagttattc aaaaagtcat ggaaaaagct ctcagtttaa atagcgttgg tcattatcaa
     3181 gagattctaa aagctttgag tgacaatatg cagaccaata ttgatttgtc tgcaaaaagt
     3241 atccctaact tgctaggcta taaagattca tttaaaacca ttgaaactca gcagttgcag
     3301 ggtgaaggag agatacttca aggtgtttct taccagattg tttcgagagc acatatgttg
     3361 gaaatgcaaa atatactccg acgttctttg ggacaagaag aagttactca gcttgaaacc
     3421 aatgcggttt tatttgaaga tttatttggc agagcacctg ttggtgatga agataattaa
     3481 cttttgatac aaataaaaaa atcaatcgta ggaaaattcc tgcgattttt tcaaaaaaat
     3541 acgaatagat aggtaggagg aaacatgaaa gcagaaatca ttgctgttgg aacagagatt
     3601 ttgacaggac agattgtcaa caccaatgcc cagtttttgt cggaaaaact agctgagatt
     3661 ggggtagatg tatattttca gacggctgta ggagacaatg aagttcgtct cttgtctttg
     3721 cttgagattg ccagtcaacg tagcagtctg gtgattttga caggcggttt ggggccaact
     3781 gaggacgacc taaccaaaca aaccctagct aaatttttag ggaaagcatt agtctttgat
     3841 cctcaggctc aggagaagtt ggatatcttt tttgccctgc gaccagacta tgcccgaaca
     3901 ccaaataacg aaagacaagc tcaaattgta gaaggagcga ttccactgcc aaacgaaaca
     3961 ggactggctg ttggaggaaa attagaagta gacggagtga cctatgtcgt ccttccaggt
     4021 ccgccaagtg aattgaaacc catggtctta aaccaacttc tacccaagtt gatgacaggg
     4081 agcaagctgt attcccgagt tcttcgtttc tttgggattg gcgagagcca gttggttacg
     4141 attttggctg atttaattga taatcagatc gatcctacct tggcccctta tgccaagaca
     4201 ggagaagtca ctctacgtct gtcaacaaag gctagcagtc aagaagaggc gaatcaagcg
     4261 ctggatatct tggaaaatca aatcttggac tgccagactt tcgaaggaat ttctttacga
     4321 gacttttgct atggttatgg ggaagaaact agtttagcca gcattgtggt agaagaactg
     4381 aaaaggcaag ggaaaaccat cgcggctgca gagagtttga cggcaggtct tttccaagct
     4441 accgtggcga atttttctgg agtttcaagt atatttgagg gtggttttgt gacctatagc
     4501 ttggaggaaa aatcaaggat gttggatatt cctgccaaga atttggaaga acatggtgtg
     4561 gtgtctgaat ttacagctca gaagatggct gagcaggcac gaagcaagac ccagtctgat
     4621 tttggaatta gtttgactgg agtggcagga ccagatagcc tagaaggaca cccagttggg
     4681 acagtcttca taggcttggc gcaagatcaa ggaactgagg ttatcaaggt taatattgga
     4741 ggcagaagcc gagcagatgt acgtcacatt gcggttatgc atgcctttaa cctagttcgc
     4801 aaggctttat taagtgacta acttttgata taatagtaga taggtctgag gatcattaga
     4861 atgtaggaga atagaatggc gaaaaaacca aaaaaattag aagaaatttc aaaaaaattt
     4921 ggggcagaac gtgaaaaggc cttgaatgac gctcttaaat tgattgagaa agactttggt
     4981 aaaggatcaa tcatgcgttt gggtgaacgt gcggagcaaa aggtgcaagt gatgagctca
     5041 ggttctttag ctcttgacat tgcccttggc tcaggtggtt atcctaaggg acgtatcatc
     5101 gaaatctatg gcccagagtc atctggtaag acaacggttg cccttcatgc agttgcacaa
     5161 gcgcaaaaag aaggtgggat tgctgccttt atcgatgcgg aacatgccct tgatccagct
     5221 tatgctgcgg cccttggtgt caatattgac gaattgctct tgtctcaacc agactcagga
     5281 gagcaaggtc ttgagattgc gggaaaattg attgactcag gtgcagttga tcttgtcgta
     5341 gtcgactcag ttgctgccct tgttcctcgt gcggaaattg atggagatat cggagatagc
     5401 catgttggtt tgcaggctcg tatgatgagc caggccatgc gtaaacttgg cgcctctatc
     5461 aataaaacca aaacaattgc catttttatc aaccaattgc gtgaaaaagt tggagtgatg
     5521 tttggaaatc cagaaacaac accgggcgga cgtgctttga aattctatgc ttcagtccgc
     5581 ttggatgttc gtggtaatac acaaattaag ggaactggtg atcaaaaaga aaccaatgtc
     5641 ggtaaagaaa ctaagattaa ggttgtaaaa aataaggtag ctccaccgtt taaggaagcc
     5701 gtagttgaaa ttatgtacgg agaaggaatt tctaagactg gtgagctttt gaagattgca
     5761 agcgatttgg atattatcaa aaaagcaggg gcttggtatt cttacaaaga tgaaaaaatt
     5821 gggcaaggtt ctgagaatgc taagaaatac ttggcagagc acccagaaat ctttgatgaa
     5881 attgataagc aagtccgttc taaatttggc ttgattgatg gagaagaagt ttcagaacaa
     5941 gatactgaaa acaaaaaaga tgagccaaag aaagaagaag cagtgaatga agaagttacg
     6001 cttgacttag gcgatgaact tgaaatcgaa attgaagaat aagctgttaa agcagtggag
     6061 aaatccgcta ctttttcgat ttttgattca agtttttaga ttatatatag tag
//
LOCUS       DAVGJL010000110         6040 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.111, whole genome shotgun sequence.
ACCESSION   DAVGJL010000110 DAVGJL010000000
VERSION     DAVGJL010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6040
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.111"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            183..383
                     /locus_tag="VVE42_001729"
     CDS             183..383
                     /locus_tag="VVE42_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073888.1"
                     /translation="MVASASASSTSTQAQEQVDKSELRALSQELDQRLKALATVSDPK
                     IDATQAVLLDAQKAPEDSALTE"
     gene            425..829
                     /locus_tag="VVE42_001730"
     CDS             425..829
                     /locus_tag="VVE42_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073889.1"
                     /translation="MDSLKDVKANASDSKPAQDKKDAKQGTEDSKDSDKMTETNSVPA
                     GVIVVSLLALLGVIAFWLIRRKKESEIQQLSTELIKVLGQLDAEKADKKVLAKAQNLL
                     QETLDFVKEENGSAETETKLVEELKAILDKLK"
     gene            1060..1767
                     /gene="ccdA2"
                     /locus_tag="VVE42_001731"
     CDS             1060..1767
                     /gene="ccdA2"
                     /locus_tag="VVE42_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEU3073890.1"
                     /translation="METIVFLISVFLAGVLSFFSPCIFPLLPVYAGILLDDQESAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYGMGAIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDSNRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMALPFLVLALASGLVMPYFSKIKRHMMLLKKIGGFLIVLM
                     GILLLLGQVNVLAGIFE"
     gene            1778..2335
                     /gene="sdbB"
                     /locus_tag="VVE42_001732"
     CDS             1778..2335
                     /gene="sdbB"
                     /locus_tag="VVE42_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HEU3073891.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGEEETKKTQAAQQPKQQTTVQQIA
                     VGKDAPDFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDF
                     EILTVIAPGIQGEKTVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDS
                     QGKIGKIQFGAISNADAETAFKEMN"
     gene            2710..4101
                     /locus_tag="VVE42_001733"
     CDS             2710..4101
                     /locus_tag="VVE42_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU3073892.1"
                     /translation="MNIFRTKNVSLDKTEMHRHLKLWDLILLGIGAMVGTGVFTITGT
                     AAATLAGPALVISIVISALCVGLSALFFAEFASRVPATGGAYSYLYAILGEFPAWLAG
                     WLTMMEFMTAISGVASGWAAYFKGLLSQYGIALPQALNGTFNPQAGTFVDLLPILVLV
                     LVTSLVLLNAKAALRFNSILVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGA
                     STGIMAGASLMFFGFLGFESISMAVDEVNTPQKNIPRGIVLSLSIVTILYALVTLVLT
                     GVVHYSHLNVDDAVAFALRSVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLAR
                     DGLVPAAFKELTKTSKVPKNATILTGLASAVAAGMFPLASIAAFLNICTLAYLIMLAY
                     GLIRLRKEKGMPKAGEFKTPLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSII
                     YFTYGYKHSTIEE"
     gene            4244..5161
                     /gene="adcAII"
                     /locus_tag="VVE42_001734"
     CDS             4244..5161
                     /gene="adcAII"
                     /locus_tag="VVE42_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HEU3073893.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDADVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLERVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDSEHKETYQKNAQAFIKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLTEIQEFVKTYKVKTIFTESNASSKVAETLVKSTGVGLKTL
                     NPLESDPQNDKTYLENLEENMSILAEELK"
     gene            5169..>6040
                     /gene="phtD"
                     /locus_tag="VVE42_001735"
     CDS             5169..>6040
                     /gene="phtD"
                     /locus_tag="VVE42_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtD"
                     /protein_id="HEU3073894.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRHQAGQVKKESNRVSYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVDGKYYVYLKDAAHADNIRTKEEIKRQKQEH
                     SHNHGGGSNDQAVVAARAQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKNE
                     LSASELAAAEAYWNGKQGSRPSSSSSHNANPAQPRLSENHNLTVTPTYHQNQGENISS
                     LLRELYAKPLSERHV"
BASE COUNT         1841 a         1089 c         1288 g         1822 t
ORIGIN      
        1 atcggttatt atagtaaaat gaaataagaa caggacaaat cgatcaggac agtaaaatcg
       61 aattctaaca atgttttaga agcctatatg tactattcta gtttcaatat accataactg
      121 ctcctagtca acctgcaagt cctgtcccag ctccaagctc agctccgact caagatccta
      181 gagtggtagc aagcgcttcg gctagctcaa cgagcacaca agctcaagag caagttgaca
      241 agtctgaact tcgtgccttg agtcaagagt tagaccaacg cttgaaagct ttggcaacag
      301 tatctgatcc gaaaatcgat gcaacccagg ctgtcctcct agatgctcaa aaagctccgg
      361 aagatagtgc cttgacagag taaggtctgc atactgcagt agagtctgtg aaggctgggc
      421 tgccttggac tctctcaaag atgtgaaagc aaatgctagc gacagcaagc ctgcacagga
      481 caagaaggat gcaaaacaag gaacggaaga tagtaaggat tcagataaga tgactgaaac
      541 aaactcagtt ccggcaggag tgattgtggt cagtctactt gccctcctag gcgtgattgc
      601 cttctggctg attcgccgta agaaagagtc agaaatccag caattaagca cggaattgat
      661 caaggttcta ggacagctag atgcagaaaa agcggataaa aaagtccttg ccaaagccca
      721 aaaccttctc caagaaaccc ttgatttcgt gaaagaagaa aatggctcag cagagacaga
      781 aactaaacta gtagaggagc ttaaagcaat ccttgacaaa ctcaagtaaa acatgattta
      841 tgaaatgtca gacctaaaag acattttcgt tcacaaaagc agcgatgagt aatccttgtc
      901 gttgcttttt ggctattttg ggggataaga tccgccttga ctagcaaaag aaaagattgg
      961 tccaatagtg cccgatacga ttttgcgtag cagaaagctt acaaataaaa ttgaatatag
     1021 taaaatagga ttagaattat taagaaagtt ggttctttat tggaaacgat agtattttta
     1081 atctctgttt ttctagcagg tgttttatcc tttttttctc cttgtatttt tcctcttctg
     1141 ccagtctatg ctgggatttt attggatgat caagaaagtg caaaaagctt ttctttgttt
     1201 gggagaaagg ttctctggtc aggcttgatt cgaacacttt gctttatcgc tggtatctct
     1261 ctcattttct ttattctagg ctttggtgct ggttactttg gtcatattct ctatgcaaat
     1321 tggtttcgat atggcatggg agctattatt atcattttgg gtcttcacca gatggaaatt
     1381 tttcatttga agaaattaga agttcaaaaa agttttacct ttaaaaaatc agattctaat
     1441 cgttattggt cagctttttt acttggtatt acctttagct ttggttggac accttgtatt
     1501 ggtccagttt taagttctgt tttagcactt gcggcttctg gaggcaatgg cgcttggcaa
     1561 ggagcgattt atactctcat ttacactctg ggcatggccc ttcctttctt ggtattggca
     1621 ctagcttcag gtctagtcat gccatatttt agtaaaatca agcgtcatat gatgctacta
     1681 aagaaaattg gtggtttcct cattgtttta atgggaattt tgttactatt aggacaagta
     1741 aatgttctag ctggaatttt tgaataaagg aggacaaatg aaaaaagtaa tgtttgctgg
     1801 cttaagtctc ttgtcattag ttgtattgat ggcctgtggt gaggaagaaa ctaaaaagac
     1861 tcaagcagca caacagccaa aacaacaaac gactgtacaa caaattgctg ttggaaaaga
     1921 tgctccagac ttcacattgc aatccatgga tggcaaagaa gttaagttat ctgattttaa
     1981 gggtaaaaag gtttacttga agttttgggc ttcatggtgt ggtccatgca agaaaagtat
     2041 gccagagttg atggaactag cggcgaaacc agatcgtgat ttcgaaattc ttactgtcat
     2101 tgcaccagga attcaaggtg aaaaaactgt tgagcaattc ccacaatggt tccaggaaca
     2161 aggatataag gatatcccag ttctttatga taccaaagca accaccttcc aagcttatca
     2221 aattcgaagc attcctacag aatatttaat tgatagccaa ggaaagattg gaaagattca
     2281 atttggtgct atcagtaatg cggatgcaga aacagcattt aaagaaatga actagaatcg
     2341 ataaaatctg attacaagat gtagtcagat tttggtaact tctaaaacta acttccagtt
     2401 tcccacaacc tagcttttag tatgagaaaa acgcgtgaca tcagtggaaa tccgtcttag
     2461 actaacttcc actggttttc tttttcttga tatataaggg ttcaaaagcg aaaagactca
     2521 gaaaaaagtg cacgacaaat agccctaaaa cagagtcgtt ttagagtaac ttccagtttt
     2581 gggagagaga tggaagttac tttgagaagt tacggtagtc agattttttt agaaaaacat
     2641 tttataaata ttcacatatc tcctatattt atggtaaaat agaatcatca gtttattttg
     2701 gagtcaaaga tgaatatatt tagaacaaag aatgttagtt tagataaaac agagatgcat
     2761 aggcatttga agttatggga tttgattttg ctgggtattg gagccatggt agggacaggt
     2821 gtctttacaa tcacaggtac tgcagctgca acacttgctg gcccagctct agtgatttca
     2881 atcgttattt ctgccttgtg tgtgggatta tcagccctct tttttgcaga atttgcctcg
     2941 cgagtacccg ctacaggagg tgcctatagt tacctctatg ctatcttagg agaattccct
     3001 gcctggttgg ctggttggtt aaccatgatg gagttcatga cagccatatc aggcgtagct
     3061 tcgggttggg cagcttattt taaaggcttg ctctctcaat acgggatagc ccttcctcag
     3121 gctttaaatg gtacctttaa tcctcaagca gggacatttg ttgatttatt gcctattctt
     3181 gtcttggtct tggtgacttc gcttgtttta ctaaatgcca aggcagcctt acgctttaat
     3241 tcgattctag ttattttgaa attttccgct ttagctctct ttgttttagt aggaatttgg
     3301 aatattaagt ttgataattg gagtaatttt gctccttacg gttttggaca aatctatgga
     3361 gctagtactg gtattatggc tggtgcgtcc ttgatgttct tcggtttttt gggatttgaa
     3421 tccatctcta tggctgtaga tgaagtcaat actcctcaaa aaaatattcc tagagggatt
     3481 gtcttatcgc tttctatcgt aaccattctc tatgccttgg tgacccttgt tttgactggt
     3541 gtggttcact atagtcattt aaatgtcgat gatgccgttg cctttgccct tcgtagtgtt
     3601 gggattagtt gggcagccaa ctatgtgtca ttagtggcta tcttgacctt gattacagtt
     3661 tgtatttcga tgacctatgc cctatcgcgt atgatttaca gtttagcacg tgacggctta
     3721 gtgcctgctg cctttaagga actgacgaag actagcaagg taccaaagaa tgctactatc
     3781 ttgacaggtc tagcttcagc agtagcagca ggaatgttcc cactagccag tattgcagcc
     3841 tttttaaata tttgtacctt agcctacttg atcatgctgg cttatggtct gattcgctta
     3901 cggaaagaaa aaggaatgcc caaagctggg gaatttaaaa caccattggt acctctgtta
     3961 ccaattttat cgatcatcat ttgtttatcc ttcatgttac agtataatat gaacacctgg
     4021 attgctttcc tagttgcgtt gttagtagga agtattattt acttcactta tggctataag
     4081 cattctacca tagaagaata aagtgaaagt ctattgagtt gttgacactc agtagatttt
     4141 tatatgtgaa agaaatttca aattttttct aaaaatatct tgacagatta aattttcagg
     4201 agtagaatat ttactagtta attaaatggt taaggagttg ttcatgaaga aacaaaattt
     4261 atttttagtc ctgttaagtg tctttctttt gtgcttgggg gcttgtggtc aaaaggaaag
     4321 tcagacagga aaggggatga aaattgtgac cagtttttat cctatctacg ctatggttaa
     4381 ggaagtatct ggtgacttga atgatgttcg gatgattcag tcaagtagtg gtattcactc
     4441 ctttgaacct tcggcaaatg atatcgcagc catctatgat gcagatgtct ttgtttacca
     4501 ttctcataca ctcgaatctt gggcaggaag tctggatcca aatctaaaaa aatccaaagt
     4561 gaaggtctta gaggcttctg agggaatgac cttggaacgt gtccctggac tagaggatgt
     4621 ggaagcaggg gatggagttg atgaaaaaac gctctatgac cctcacacat ggctagatcc
     4681 tgaaaaagct ggagaagaag cccaaattat cgctgataaa ctttcagagg tggatagtga
     4741 gcataaagag acttatcaaa aaaatgcgca agcctttatc aaaaaagctc aggaattgac
     4801 taagaaattc caaccaaaat ttgaaaaagc gactcagaaa acatttgtaa cacaacatac
     4861 agccttttct tatctagcga agagatttgg gcttaatcaa cttggtattg caggtatctc
     4921 tcctgaacaa gaaccaagtc cacgacaact aacagaaatt caggaatttg ttaagaccta
     4981 taaggttaaa acgattttta cagaaagtaa cgcttcttca aaagtagctg aaactcttgt
     5041 caaatcaaca ggtgtgggtc ttaaaactct gaatccttta gagtcagacc cacaaaatga
     5101 caagacctat ttagaaaatc ttgaagaaaa tatgagtatt ctagcagaag aattaaagtg
     5161 aggaaagaat gaaaatcaat aaaaaatatc tagcaggttc agtggcagtc cttgccctaa
     5221 gtgtttgttc ctatgaactt ggtcgtcacc aagctggtca ggttaagaaa gagtctaatc
     5281 gagtttctta tatagatggt gatcaggctg gtcaaaaggc agaaaacttg acaccagatg
     5341 aagtcagtaa gagggagggg atcaacgccg aacaaatcgt catcaagatt acggatcaag
     5401 gttatgtgac ctctcatgga gaccattatc attactataa tggcaaggtc ccttatgatg
     5461 ccatcatcag tgaagagctc ctcatgaaag atccgaatta tcagttgaag gattcagaca
     5521 ttgtcaatga aatcaagggt ggttatgtta tcaaggtaga tggaaaatac tatgtttacc
     5581 ttaaggatgc agctcatgcg gataatattc ggacaaaaga agagattaaa cgtcagaagc
     5641 aggaacacag tcataatcac gggggtggtt ctaacgatca agcagtagtt gcagccagag
     5701 cccaaggacg ctatacaacg gatgatggtt atatcttcaa tgcatctgat atcattgagg
     5761 acacgggtga tgcttatatc gttcctcacg gcgaccatta ccattacatt cctaagaatg
     5821 agttatcagc tagcgagtta gctgctgcag aagcctattg gaatgggaag cagggatctc
     5881 gtccttcttc aagttctagt cataatgcaa atccagctca accaagattg tcagagaacc
     5941 acaatctgac tgtcactcca acttatcatc aaaatcaagg ggaaaacatt tcaagccttt
     6001 tacgtgaatt gtatgctaaa cccttatcag aacgccatgt
//
LOCUS       DAVGJL010000111         5924 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.112, whole genome shotgun sequence.
ACCESSION   DAVGJL010000111 DAVGJL010000000
VERSION     DAVGJL010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5924
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.112"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            1..975
                     /locus_tag="VVE42_001736"
     CDS             1..975
                     /locus_tag="VVE42_001736"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEU3073895.1"
                     /translation="MSISAGIVTFLLTLVGIPAFIQFYRKAQITGQQMHEDVKQHQAK
                     AGTPTMGGLVFLIAAVVVSFLVALFSKQLTNNVGMVLFILVLYGLVGFLDDFLKVFRK
                     INEGLNPKQKLALQLLGGVIFYLFYERGGDMLSVFGYQVHLGIFYIIFALFWLVGFSN
                     AVNLTDGIDGLASISVVISLSAYGVIAYVQGQMDILLVILAVIGGLLGFFIFNHKPAK
                     VFMGDVGSLALGGMLAAISMALHQEWTLLIIGIVYVFETTSVMMQVTYFKMTGGKRIF
                     RMTPVHHHFELGGLSGKGNPWSEWKVDLFFWGVGLLASLLTLAILYLM"
     gene            1417..3522
                     /locus_tag="VVE42_001737"
     CDS             1417..3522
                     /locus_tag="VVE42_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU3073896.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTPEEF
                     AHYRATGQLPGNAEVDGKMPQQASGMKQDGVLAKLGRNLTAEAREGKLDPVIGRNKEI
                     QETSEILSRRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGL
                     EAGTQYRGSFEENVQNLVNEVKEAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAKDTYKILQGIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     ETEKDKQEKAVEAEDFEAALNYKTRIAELEKKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMGASDIERLKDMAHRLQDKVIGQDKAVEAVARAIRRNRAGFDEGNRPIGSFLF
                     VGSTGVGKTELAKQLALDMFGTQDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVIIATS
                     NAGFGYEANLTEDADKPELMDRLKPFFRPELLNRFNAVIEFSHLTKEDLSKIVDLMLA
                     EVNQTLAKKDIDLVVSQAAKDYITEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     AKHLEADMEDGVLVIREKV"
     gene            complement(3818..4300)
                     /locus_tag="VVE42_001738"
     CDS             complement(3818..4300)
                     /locus_tag="VVE42_001738"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEU3073897.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAKIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWAKLILEQGISDDAFERHVI
                     "
     gene            complement(4395..5879)
                     /locus_tag="VVE42_001739"
     CDS             complement(4395..5879)
                     /locus_tag="VVE42_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEU3073898.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYAGQPAADAFRNQLEKNGIDSYLHYPIKGYPTDMDHIISPEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSNMYHDQINGIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMLERILGKSPYA
                     SPTDMGVNMVGFAITDDEAAVEASKQEIIRRYYQTVLDFKAEKVGEAAVKKIELLMND
                     LGITPADRKVAVVARQKAEETGGPALAFELPNGEIITGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDAARAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
BASE COUNT         1666 a         1108 c         1396 g         1754 t
ORIGIN      
        1 atttccatca gtgctggaat tgtgacattt ttactaactt tagtaggaat tccggccttt
       61 atccaatttt atagaaaggc gcaaattaca ggccagcaga tgcatgagga tgttaaacag
      121 caccaggcaa aagctgggac tcctacaatg ggagggcttg ttttcctcat tgctgcagtt
      181 gtggtgagtt tcctcgtcgc tcttttttcg aaacaattga ccaataatgt tggtatggtt
      241 ttgtttattt tggtcttgta tggcttggtt ggttttttag atgactttct caaggtcttc
      301 cgaaagatca atgaggggct aaaccccaaa cagaaattgg ctcttcagct tctaggtggt
      361 gttattttct accttttcta tgaacgtggg ggcgatatgc tctctgtctt tggctaccaa
      421 gtgcatttag ggattttcta tattatcttc gctcttttct ggctagttgg tttttcaaac
      481 gcagtcaact tgacagacgg aattgacggt ctggctagta tttcagtggt gattagcttg
      541 tctgcttatg gagttattgc ctatgtgcaa ggtcagatgg atattcttct agtaattctt
      601 gccgtgattg gtggtttgct cggtttcttc atctttaatc acaagcctgc caaggtcttt
      661 atgggagatg tgggaagttt ggctctcggt ggaatgctgg cagctatctc tatggccctc
      721 caccaagaat ggactctctt gattatcgga attgtttatg tctttgaaac aacttcggtt
      781 atgatgcaag taacttattt caaaatgact ggtggtaaac gtattttccg tatgacacct
      841 gtccatcacc attttgagct tggaggattg tctggtaaag gaaatccttg gagcgagtgg
      901 aaggttgacc tcttcttttg gggagtgggg cttctagcaa gtctcctaac cctcgcaatt
      961 ttatatttga tgtaagaatg gcaccctgat gtttcagggt gtttttgctt tttaaaaaat
     1021 attggtcaat taaagtcaaa ttgcttgacc ttttctgact aatgtagtat attatgaacg
     1081 taaggtaaat gaaagcctta ctagaacact tatactcaat gaaaatcaac gagcaaacta
     1141 ggaagtgctc aaaacacggt tttgaggtta cagatagaac tgacgtggtt tgaagagatt
     1201 ttcgaagagt attatttaaa gtaaaataga aagaggtggg gtctatgttt catctagaaa
     1261 tctttagaag taaagatagt ctactcctgc ttgaaaaaga aaaaacagaa atagtaccta
     1321 gagtagcaat ctagctagtc taaatttttt aaaataaagg tcaaagatag tcaatatcag
     1381 taatcataac taagtaaata aaaagaggta aagaatatga acaacaactt taataatttt
     1441 aacaacatgg atgatttatt taaccaattg atgggtggta tgcgaggata tagttctgaa
     1501 aatcgccgtt acttgattaa tggtcgtgaa gtgacacctg aggaatttgc tcactatcgt
     1561 gcaactggtc aattgccagg aaatgcagaa gttgatggaa aaatgccaca acaggcatca
     1621 ggtatgaagc aagacggtgt ccttgctaaa ctgggtcgaa acttgacagc agaagcgcgt
     1681 gagggcaagc tggatcctgt tatcggacga aataaggaaa ttcaagaaac atctgaaatc
     1741 ctttcacgcc gtaccaagaa caatcctgta cttgtcggag atgcaggtgt tggtaagaca
     1801 gccgttgtcg aaggtttagc acaagctatt gtgaatggcg atgttcctgc tgctattaag
     1861 aataaggaaa ttatttccat tgacatctca ggtcttgagg ctgggactca atatcgtggt
     1921 agctttgaag aaaatgtcca aaacctagtc aatgaagtga aagaagcagg gaatattatc
     1981 ctcttctttg atgaaattca ccaaatcctt ggcgctggta gcactggtgg agacagtggt
     2041 tctaaagggc ttgcggatat tctcaagcca gctctctctc gtggagaatt gacagtgatt
     2101 ggggcaacga ctcaagatga ataccgtaat accatcttga agaatgcagc tcttgctcgt
     2161 cgtttcaacg aagtcaaggt caatgctcct tcagcaaagg atacatataa aatccttcaa
     2221 ggaattcgtg atctatatca acaacaccac aatgtcatct tgccagacga agttttgaaa
     2281 gcagcggtgg attattctgt tcagtatatt cctcaacgta gcttgccaga taaggctatt
     2341 gaccttgtcg atgttacggc agctcacttg gcagctcagc atcctgtaac agatgtgcat
     2401 gctgttgaac gagaaatcga aacggaaaaa gacaagcaag aaaaagcagt tgaagcagaa
     2461 gattttgaag cagctctaaa ctataaaaca cgcattgcag aattggaaaa gaaaatcgaa
     2521 aaccacacag aagatatgaa agtgactgca agtgtcaacg atgtggctga atccgtggaa
     2581 cgaatgacag gtatcccagt atcgcaaatg ggagcttcag atatcgaacg tttgaaagat
     2641 atggctcatc gcttgcaaga caaggtgatt ggtcaagata aggccgtaga agctgtagct
     2701 cgtgctatcc gtcgtaaccg tgctggtttt gatgaaggaa atcgcccaat cggcagcttc
     2761 ctctttgtag gatctactgg ggttggtaag acggagcttg ctaagcaatt ggcactcgat
     2821 atgtttggaa cccaggatgc gattatccgt ttggatatgt ctgaatacag tgaccgcaca
     2881 gctgtttcta agctaattgg tacaacagca ggctatgtgg gttatgatga caatagcaat
     2941 accttaacag aacgtgttcg tcgcaatcca tactctatca ttctcttgga tgaaattgaa
     3001 aaggctgatc ctcaagttat tacccttctc ctccaagttc tagatgatgg tcgtttgaca
     3061 gatggtcaag gaaatacagt aaacttcaag aacactgtca ttattgcgac ctcaaatgct
     3121 ggatttggct atgaagccaa cttgacagaa gatgcggata aaccagaatt gatggaccgt
     3181 ttgaaacctt tcttccgtcc agaactcctc aaccgcttta atgcagtcat cgagttctca
     3241 cacttgacta aggaagacct ttctaagatt gtagatttga tgttggctga agttaaccaa
     3301 accttggcta agaaagacat tgacttggta gtcagtcaag cggctaaaga ttatatcaca
     3361 gaagaaggtt acgacgaagt catgggggtt cgtcctctcc gtcgcgtggt tgaacaagaa
     3421 attcgtgata aggtgacaga cttccacttg gatcatttag atgctaaaca tctggaagca
     3481 gatatggaag atggcgtttt ggttattcgt gagaaagtct aagacagaat tttgaggata
     3541 aaaaagaagg agccagctga aaaaaactgg ttccttttga ggtatgacgg gcatgtcgta
     3601 tagtagaagt gtattattct agtttcaatt tactatagta gctcagaagt cggtacttaa
     3661 acgtgctata tcaaaaccag tccttgaaaa acgtggactg gtttcgtgtt tggattatta
     3721 ccttgaacga catgcgttaa aagttagttg aaccgccgta tgccgaacgg cacgtacggt
     3781 ggtgtgagag gggctagaga ttatccccta ctcgatttta aatcacatga cgttcaaagg
     3841 catcatctga aatcccttgt tccaagatga gttttgccca ttctttagca gagaagaggc
     3901 tgtggtcctt gtagtttccg caagattcga tggttgtccc tgggacatct tcccaagtag
     3961 tagtttcagc gatttccttg agcgaatcct tgataacagc tgcgatttta gcactggtgt
     4021 gacgtcccca cataatcatg tggaagcctg tgcggcaacc aaatggtgaa cagtcaatca
     4081 tgccgtcaat gcgggtacgg atgagtttgg ctaagaggtg ctcgatagtg tgaaggccgg
     4141 cagtagggat agagtcttcg tttggttgca ccaagcgaat atcataattg gagatgatgt
     4201 ctccttttgg tcctgtttct tccccaatca agcgaacata gggtgctttg acaatggtgt
     4261 ggtcaagttc aaaactttcg acaataactt cttttgacat ggtaaatcct ttcagttttc
     4321 ttctctcatt atatcataaa ggttgctcct gagacagaga gaaaacctct ccgaggctgg
     4381 agaggttgaa atctttactt acgatataag cggtcgtatt ggtagtatgg gtcaaaggtt
     4441 acgttgatac ccagtttacg aaggacattc ttgtcttcat cagtcaagat gatggttgag
     4501 tgggcttcgc ttcctttgag gttgccgagt tcttccatag cgcgggcagc atcaggattt
     4561 tctgtagctg tgatagcaag tgcaatcagg atttcatttg aatgaaggcg tggattgcgg
     4621 ctaccgagat gatcgatttt aagaccttgg attggcttaa caacttcagg ctcgattagt
     4681 tttacttctt tagcgatgtc agctgatttt ttgatggcat tgatcaaggc agcggctgta
     4741 gggccaaaga gttctgagtt cttaccagtg atgatttccc catttggcaa ttcaaaggct
     4801 agggctggtc caccagtttc ttctgctttt tggcgcgcaa cgacagcaac cttacggtct
     4861 gcaggtgtga taccgaggtc gttcatgagc aactcaattt tcttgacggc agcttcgcca
     4921 actttttcag ctttgaagtc aagaactgtt tgatagtaac ggcggatgat ttcttgttta
     4981 gaagcttcga cagcagcctc gtcatctgta atagcgaaac caaccatgtt gacacccata
     5041 tctgtcggtg aagcgtatgg tgattttccg agaatacgtt ccaacatgcg cttgagcact
     5101 gggaagattt cgatatcacg gttgtagttg acagtggttt ctccataggt ttgaagatgg
     5161 aaggggtcaa tcatgttgac atcatcaagg tcagctgtgg cagcttcata agccaagtta
     5221 actggatgat gaaggggaag attccaaaca gggaaggttt caaatttagc gtagccagat
     5281 ttgatgccat tgatttggtc gtggtacata ttggacatac acgttgccaa ttttccagaa
     5341 ccgggtccag gagcggttac gacaatcaag ttgcgactgg ttttgatgta gtcgtttttg
     5401 cccatgcctt ctggggaaat gatgtgatcc atatccgtcg gatatccttt gattggataa
     5461 tgaagataag aatcaattcc gtttttctca agttggttgc ggaaggcatc tgcagcgggt
     5521 tggccagcgt attgtgtaat gacaacggaa ccaacaaaaa tccctaattc attgaatttg
     5581 tcaatcaaac gaagaacttc ttggtcataa gaaatgccca agtcgccacg tgctttggaa
     5641 tgttcaatgt tgctagcatt aatggcaatc acaacctcaa cctgctcttt caattcttgc
     5701 aagagcttga ttttgttgtc aggttcataa ccaggaagga cacgagcagc gtggaaatct
     5761 tctaacattt tgccaccaaa ctctaggtaa agcttgccgt caaattggtt aatgcgctct
     5821 aagatgtggt cgcgttgtaa attcaaatac tgttcagaac taaaagcttg ttttttcatt
     5881 tttttacctc tggactctat tataataaaa aattggaagt tagg
//
LOCUS       DAVGJL010000112         5806 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.113, whole genome shotgun sequence.
ACCESSION   DAVGJL010000112 DAVGJL010000000
VERSION     DAVGJL010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5806
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.113"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            2..268
                     /locus_tag="VVE42_001740"
     CDS             2..268
                     /locus_tag="VVE42_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEU3073899.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            261..629
                     /locus_tag="VVE42_001741"
     CDS             261..629
                     /locus_tag="VVE42_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEU3073900.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGGVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATKLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            749..2017
                     /locus_tag="VVE42_001742"
     CDS             749..2017
                     /locus_tag="VVE42_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HEU3073901.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFGIY
                     FREKLSSPMVYGEVPVYANEDLVVESGKLTPKTSFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKVWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQD
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            2014..3291
                     /gene="tilS"
                     /locus_tag="VVE42_001743"
     CDS             2014..3291
                     /gene="tilS"
                     /locus_tag="VVE42_001743"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEU3073902.1"
                     /translation="MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETIFMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYD
                     LAIAELSNNINVEDLQQLFSYSESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKSKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEKEDELVLHYQNQVAYQGYLFSFGLPLEG
                     ELIQQIPVSRETSIHIRHRKTGDVLIKNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIMNTVLYIEKIDR"
     gene            3295..3837
                     /gene="hpt"
                     /locus_tag="VVE42_001744"
     CDS             3295..3837
                     /gene="hpt"
                     /locus_tag="VVE42_001744"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEU3073903.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKEREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVLKEEVYSN"
     gene            3853..>5806
                     /gene="ftsH"
                     /locus_tag="VVE42_001745"
     CDS             3853..>5806
                     /gene="ftsH"
                     /locus_tag="VVE42_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEU3073904.1"
                     /translation="MKKQNNGLIKNPFLWLLFIFFLVTGFQYFYSGNNSGGSQQINYT
                     ELVQEITDGNVKELTYQPNGSVIEVSGVYKNPKTSKEGTGIQFFTPSVTKVEKFTSTI
                     LPADTTVSELQKLATDHKAEVTVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGG
                     GNGRNPMSFGRSKAKAANKEDIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAAPAIIFIDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFV
                     GADLENVLNEAALVAARRNKSIIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGR
                     VAEEIIFNVQTTGASNDFEQATQMARAMVTEYGMSEKLGPVQYEGNHAMLGAQSPQKS
                     ISEQTAYEIDEEVRSLLNEARNKAAEIIQSNRETHKLIAEALLKYETLDSTQIKALYE
                     TGKMPEAVEEESHALSYDEVKSKMNDE"
BASE COUNT         2023 a          863 c         1188 g         1732 t
ORIGIN      
        1 gatgagatta gataaatatt taaaagtatc gcgaattatc aagcgtcgta cagtcgcaaa
       61 ggaagtagca gataaaggta gaatcaaggt taatggaatc ttggccaaaa gttcaacgga
      121 cttgaaagtt aatgaccaag ttgaaattcg ctttggcaat aagttgctgc ttgtaaaagt
      181 actagagatg aaagatagta caaaaaaaga agatgcagca ggaatgtatg aaattatcag
      241 tgaaacacgg gtagaagaaa atgtctaaaa atattgtaca attgaataat tcttttattc
      301 aaaatgaata ccaacgtcgt cgctacctga tgaaagaacg acaaaaacgg aatcgtttta
      361 tgggaggggt attgattttg attatgctat tatttatctt gccaactttt aatttagcgc
      421 agagttatca gcaattactc caaagacgtc agcaattagc agacttgcaa actcagtatc
      481 aaactttgag tgatgaaaag gataaggaga cagcatttgc taccaagttg aaagatgaag
      541 attatgctgc taaatataca cgagcgaagt actattattc taagtcgagg gaaaaagttt
      601 atacgattcc tgacttgctt caaaggtgat aaaatggaaa atttattaga cgtaatagag
      661 caatttttga gtttgtcaga tgaaaagctg gaagaattgg ctgataaaaa tcaattattg
      721 cgtttacaag aagaaaagga aaggaagaat gcgtaaattc ttaattattt tgttgctacc
      781 aagttttttg accatttcaa aagtcgttag cacagaaaaa gaagtcgtct atacttcgaa
      841 agaaatttat tacctttcac aatctgactt tggtatttat tttagagaaa aattaagttc
      901 tcccatggtt tatggagagg ttcctgttta tgcgaatgaa gatttagtag tggaatctgg
      961 gaaattgact cccaaaacaa gttttcaaat aaccgagtgg cgcttaaata aacaaggaat
     1021 tccagtattt aagctatcaa atcatcaatt tatagctgcg gacaaacgat ttttatatga
     1081 tcaatcagag gtaactccaa caataaaaaa agtatggtta gaatctgact ttaaactgta
     1141 caatagtcct tatgatttaa aagaagtgaa atcatcctta tcagcttatt cgcaagtatc
     1201 aatcgacaag accatgtttg tagaaggaag agaatttcta catattgatc aggctggatg
     1261 ggtagctaaa gaatcaactt ctgaagaaga taatcggatg agtaaagttc aagaaatgtt
     1321 atctgaaaaa tatcagaaag attctttctc tatttatgtt aagcaactga ctactggaaa
     1381 agaagctggt atcaatcaag atgaaaagat gtatgcagcc agcgttttga aactctctta
     1441 tctctattat acgcaagaaa aaataaatga gggtctttat cagttagata cgactgtaaa
     1501 atacgtatct gcagtcaatg attttccagg ttcttataaa ccagagggaa gtggtagtct
     1561 tcctaaaaaa gaagataata aagaatattc tttaaaggat ttaattacga aagtatcaaa
     1621 agaatctgat aatgtagctc ataatctatt gggatattac atttcaaacc aatctgatgc
     1681 cacattcaaa tccaagatgt ctgccattat gggagatgat tgggatccaa aagaaaaatt
     1741 gatttcttct aagatggccg ggaagtttat ggaagctatt tataatcaaa atggatttgt
     1801 gctagagtct ttgactaaaa cagattttga tagtcagcga attgccaaag gtgtttctgt
     1861 taaagtagct cataaaattg gagatgcgga tgaatttaag catgatacgg gtgttgtcta
     1921 tgcagattct ccatttattc tttctatttt cactaagaat tctgattatg atacgatttc
     1981 taagatagcc aaggatgttt atgaggttct aaaatgaggg aaccagattt tttaaatcat
     2041 tttctcaaga agggatattt caaaaagcat gctaaggcgg ttctagctct ttctggtgga
     2101 ttagattcca tgtttctatt taaggtattg tctacttatc aaaaagagtt agagattgaa
     2161 ttgattctag ctcatgtgaa tcataagcag agaattgaat cagattggga agaaaaggaa
     2221 ttaaggaagt tggctgctga agcagagctt cctatttata tcagcaattt ttcaggagaa
     2281 ttttcagaag cgcgtgcacg aaattttcgt tatgattttt ttcaagaggt catgaaaaag
     2341 acaggtgcga cagctttagt cactgcccac catgctgatg atcaggtgga aacgattttt
     2401 atgcgcttga ttcgaggaac tcgcttgcgc tatctatcag gaattaagga gaagcaagta
     2461 gtcggagaga tagaaatcat tcgtcccttc ttgcattttc agaaaaaaga ctttccatca
     2521 atttttcact ttgaagatac atcaaatcag gagaatcatt attttcgaaa tcgtattcga
     2581 aattcttact taccagaatt ggaaaaagaa aatcctcgat ttagggatgc aatcttaggc
     2641 attggcaatg aaattttaga ttatgatttg gcaatagctg aattatctaa caatattaat
     2701 gtggaagatt tacagcagtt attttcttac tctgagtcta cacaaagagt tttacttcaa
     2761 acttatctga atcgttttcc agatttgaat cttacaaaag ctcagtttgc tgaagttcag
     2821 cagattttaa aatctaaaag ccagtatcgt catccgatta aaaatggcta tgaattgata
     2881 aaagagtacc aacagtttca gatttgtaaa atcagtccgc aggctgatga aaaggaagat
     2941 gaacttgtgt tacactatca aaatcaggta gcttatcaag gatatttatt ttcttttgga
     3001 cttccattag aaggtgaatt aattcaacaa atacctgttt cacgtgaaac atccatacac
     3061 attcgtcatc gaaaaacagg agatgttttg attaaaaatg ggcatagaaa aaaactcaga
     3121 cgtttattta ttgatttgaa aatccctatg gaaaagagaa actctgctct tattattgag
     3181 caatttggtg aaattgtctc aattttggga attgcgacca ataatttgag taaaaaaacg
     3241 aaaaatgata taatgaacac tgtactttat atagaaaaaa tagataggta aaaaatgtta
     3301 gaaaacgata ttaaaaaagt cctcgtttca cacgatgaaa ttacagaagc agctaaaaaa
     3361 ctaggtgctc aattaactaa agactatgca ggaaaaaatc caatcttagt tgggatttta
     3421 aaaggatcta ttccttttat ggctgaattg gtcaaacata ttgatacaca tattgaaatg
     3481 gacttcatga tggtttctag ctaccatggt ggaacagcaa gtagtggtgt tatcaatatt
     3541 aaacaagatg tgactcaaga tatcaaagga agacatgttc tatttgtaga agatatcatt
     3601 gatacaggtc aaactttgaa gaatttgcga gatatgttta aagaaagaga agcagcttct
     3661 gttaaaattg caaccttgtt ggataaacca gaaggacgtg ttgtagaaat tgaggcagac
     3721 tatacttgct ttactatccc aaatgagttt gtagtaggtt atggtttaga ctacaaagaa
     3781 aattatcgta atcttcctta tattggagta ttgaaagagg aagtgtattc aaattagaaa
     3841 gaataatctt taatgaaaaa acaaaataat ggtttaatta aaaatccttt tctatggtta
     3901 ttatttatct ttttccttgt gacaggattc cagtatttct attctgggaa taactcagga
     3961 ggaagtcagc aaatcaacta tactgagttg gtacaagaaa ttaccgatgg taatgtaaaa
     4021 gaattaactt accaaccaaa tggtagtgtt atcgaagttt ctggtgtcta taaaaatcct
     4081 aaaacaagta aagaaggaac aggtattcag tttttcacgc catctgttac taaggtagag
     4141 aaatttacca gcactattct tcctgcagat actaccgtat cagaattgca aaaacttgct
     4201 actgaccata aagcagaagt aactgttaag catgaaagtt caagtggtat atggattaat
     4261 ctactcgtat ccattgtgcc atttggaatt ctattcttct tcctattctc tatgatggga
     4321 aatatgggag gaggcaatgg ccgtaatcca atgagttttg gacgtagtaa ggctaaagca
     4381 gcaaataaag aagatattaa agtaagattt tcagatgttg ctggagctga ggaagaaaaa
     4441 caagaactag ttgaagttgt tgagttctta aaagatccaa aacgattcac aaaacttgga
     4501 gcccgtattc cagcaggtgt tcttttggag ggacctccgg ggacaggtaa gactttgctt
     4561 gctaaggcag ttgctggaga agcaggtgtt ccattcttta gtatctcagg ttctgacttt
     4621 gtagaaatgt ttgtcggagt tggagctagt cgtgttcgct ctctttttga ggatgccaaa
     4681 aaagcagcac cagctatcat ctttatcgat gaaattgatg ctgttggacg tcaacgtgga
     4741 gtcggtctcg gcggaggtaa tgacgaacgt gaacaaacct tgaaccaact tttgattgag
     4801 atggatggtt ttgagggaaa tgaagggatt atcgtcatcg ctgcgacaaa ccgttcagat
     4861 gtacttgatc ctgccctttt gcgtccagga cgttttgata gaaaagtatt ggttggccgt
     4921 cctgatgtta aaggtcgtga agcaatcttg aaagttcacg ctaagaacaa gcctttagca
     4981 gaagatgttg atttgaaatt agtggctcaa caaactccag gctttgttgg tgctgattta
     5041 gagaatgtct tgaatgaagc agctttagtt gctgctcgtc gcaataaatc gataattgat
     5101 gcttcagata ttgatgaagc agaagataga gttattgctg gaccttctaa gaaagataag
     5161 acagtttcac aaaaagaacg agaattggtt gcttaccatg aggcaggaca taccattgtt
     5221 ggtctagtct tgtcgaatgc tcgcgttgtc cataaggtta caattgtacc acgcggccgt
     5281 gcaggcggat acatgattgc acttcctaaa gaggatcaaa tgcttctatc taaagaagat
     5341 atgaaagagc aattggctgg cttaatgggt ggacgtgtag ctgaagaaat tatctttaat
     5401 gtccaaacta caggagcttc aaacgacttt gaacaagcga cacaaatggc acgtgcaatg
     5461 gttacagagt acggtatgag tgaaaaactt ggcccagtac aatatgaagg aaaccatgct
     5521 atgcttggtg cacagagtcc tcaaaaatca atttcagaac aaacagctta tgaaattgat
     5581 gaagaggttc gttcattatt aaatgaggca cgaaataaag ctgctgaaat tattcagtca
     5641 aatcgtgaaa ctcacaagtt aattgcagaa gcattattga aatacgaaac attggatagt
     5701 acacaaatta aagctcttta cgaaacagga aagatgcctg aagcagtaga agaggaatct
     5761 catgcactat cctatgatga agtaaagtca aaaatgaatg acgaaa
//
LOCUS       DAVGJL010000113         5696 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.114, whole genome shotgun sequence.
ACCESSION   DAVGJL010000113 DAVGJL010000000
VERSION     DAVGJL010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5696
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.114"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..>5696
                     /locus_tag="VVE42_001746"
     CDS             <1..>5696
                     /locus_tag="VVE42_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU3073905.1"
                     /translation="AAVILAAYAPNEVVLADTSSSEDALSISDKEKVVVDKETENKEK
                     HKDIHNAIETSKDTEEKKTTVIEEKEVVSKNPVIDTKTSNEEARIKEDSNQSQGDNAH
                     SSANKGTENPKKEDRLVYIAEFKDKESGEKAIKGLSNLKNTKVLYTYDRIFNGSAIET
                     TPDNLDKIKQIEGISSVERAQKVQPMMNHARKEIGVEEAIDYLKSINAPFGKNFDGRG
                     MVISNIDTGTDYRHKAMRIDDDAKASMRFKKEDLKGTDKNYWLSDKIPHAFNYYNGGK
                     ITVEKYDDGRDYFDPHGMHIAGILAGNDTEQDIKNFNGIDGIAPNAQIFSYKMYSDAG
                     SGFAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRALRKAGIPMVVAT
                     GNYATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVASAKNQTVEFDKVNIGGE
                     SFKYRNIGAFFDKNKITTNEDGTKAPSKLKFVYIGKGQDQDLIGLDLRGKIAVMDRIY
                     TKDLKNAFKKAMDKGARAIMVVNTVNYYNRDNWTELPAMGYEADEGTKSQVFSISGDD
                     GVKLWNMINPNKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKPNVGDEKEIDFKFAP
                     DTDKELYKEDIIVPAGSTSWGPRIDLLLKPDVSAPGKNIKSTLNVINGKSTYGYMSGT
                     SMATPIVAASTVLIRPKLKEMLERPVLKNLKGDDKIDLTSLTKIALQNTARPMMDATS
                     WKEKSQYFASPRQQGAGLINVANALRNEVVATFKNTDSKGLVNSYGSISLKEIKGDKK
                     YFTIKLHNTSNRPLTFKVSASAITTDSLTDRLKLDETYKDEKSPDGKQIVPEIHPEKV
                     KGANITFEHDTFTIGANSSFDLNAVINVGEAKNKNKFVESFIHFESVEEMEALNSNGK
                     KINFQPSLSMPLMGFAGNWNHEPILDKWAWEEGSRSKTLGGYDDDGKPKIPGTLNKGI
                     GGEHGIDKFNPAGVIQNRKDKNTTSLDQNPELFAFNNQGINAPSSSGSKIANIYPLDS
                     NGNPQDAQLERGLTPSPLVLRSAEEGLISIVNTNKEGENQRDLKVISREHFIRGILNS
                     KSNDAKGIKSSKLKVWGDLKWDGLIYNPRGREENAPESKDNQDPATKIRGQFEPIAEG
                     QYFYKFKYRLTKDYPWQVSYIPVKIDNTAPKIVSVDFSNPEKIKLITKDTYHKVKDQY
                     KNETLFARDQKEHPEKFDEIANEVWYAGAALVNEDGEVEKNLEVTYAGEGQGRNRKLD
                     KDGNTIYEIKGAGDLRGKIIEVIALDGSSNFTKIHRIKFANRADENGMIPYYLVDPDQ
                     DSSKYQKLGEIAESKFKNLGNGKEGSLKKDTTGVEHHQGNEEATEEKSSFTIDRNIST
                     IRDFENKDLKKLIKKKFREVEDFVTGGKRTVEFDYQYDDKGNITAYDDGSALQYETEK
                     LDEIKSKIYGVLSPSKDGHFEILGKISNVSKNAKVYYANNYKSIEIKATKYDSHSKTM
                     IFDLYANINDIVDGLAFAGDMRFFVKDDDRIKAETKIRMPEKNKETKAEYPYVSSYGN
                     VIELGEGDLSKNKPDNLTKMESGKIYSDSEKQQYLLKDNIILRKGYALKVTTYNPGKT
                     DMLEGNGVYSKEDIAKIQKANPNLRVLSETTIYADSRNVEDGRSTQAVLMSALDGFNI
                     IRYQVFTFKMNDKGEAIDKDGNLVTDSSKLVLFGKDDKEYTGEDKSNVEAIKEDGSML
                     FIDTKPVNLSMDKNYFNPSKSNKIYVRNPEFYLRGKISDKGGFNWELRVNESVVDNYL
                     IYGDLHIDNTRDFNIKLNVKDGDIMDWGMKDYKANGFPDKVTDMDGNVYLQTGYSNLN
                     AKAVGVHYQFLYDNVKPEVNIDPKGNTSIEYADGKSVVFNINDKRNNGFDGEIQEQ"
BASE COUNT         2227 a          798 c         1129 g         1542 t
ORIGIN      
        1 cagctgcagt tattttagca gcctatgccc ctaatgaggt agtcttagca gacacatcta
       61 gctctgaaga tgctttaagc atctctgata aagaaaaagt agtagtagat aaggaaacag
      121 aaaataaaga gaaacataaa gatattcata atgctataga aacttcaaag gatactgaag
      181 aaaagaaaac aacagttatt gaggaaaaag aagttgttag taaaaatcct gtgatagaca
      241 ctaaaactag caatgaagaa gcaagaatca aagaagattc caatcaatcc caaggagata
      301 atgcacactc gtctgcaaat aaaggcacag aaaatccgaa aaaagaagat agacttgtct
      361 atattgctga atttaaagat aaagaatctg gagaaaaagc aatcaaggga ctatcaaatc
      421 ttaagaatac aaaagtttta tatacttatg atagaatttt taacggtagt gccatagaaa
      481 caactccaga taacttggac aaaattaaac aaatagaagg tatttcatcg gttgaaaggg
      541 cacaaaaagt ccaacccatg atgaatcatg ccagaaagga aattggagtt gaggaagcta
      601 ttgattacct aaagtctatc aatgctccat ttgggaaaaa ttttgatggt agaggtatgg
      661 tcatttcaaa tatcgatact ggaacagatt ataggcataa ggctatgaga atcgatgatg
      721 atgccaaagc ctcaatgaga tttaaaaaag aagacttaaa aggcactgat aaaaattatt
      781 ggttgagtga taaaatccct catgcgttca attattataa tggtggcaaa atcactgtag
      841 aaaaatatga tgatggaagg gattattttg acccacatgg gatgcatatt gcagggattc
      901 ttgctggaaa tgatactgaa caagatatca aaaactttaa cggcatagat ggaattgcac
      961 ctaatgcaca aattttctct tacaaaatgt attctgacgc aggatctggg tttgcgggtg
     1021 atgaaacaat gtttcatgct attgaagatt ctatcaaaca caacgttgat gttgtttcgg
     1081 tatcatctgg ttttacagga acaggtcttg taggtgagaa atattggcaa gctattaggg
     1141 cattaagaaa agcaggcatt ccaatggttg tcgctacggg taactatgcg acttctgctt
     1201 caagttcttc atgggattta gtagcaaata atcatctgaa aatgaccgac actggaaatg
     1261 taacacgaac tgcagcacat gaagatgcga tagcggtcgc ttctgctaaa aatcaaacag
     1321 ttgagtttga taaagttaac ataggtggag aaagttttaa atacagaaat ataggggcct
     1381 ttttcgataa gaataaaatc acaacaaatg aagatggaac aaaagctcct agtaaattaa
     1441 aatttgtata tataggcaag gggcaagacc aagatttgat aggtttggat cttaggggca
     1501 aaattgcagt aatggataga atttatacaa aggatttaaa aaatgctttt aaaaaagcta
     1561 tggataaggg tgcacgcgcc attatggttg taaatactgt aaattactac aatagagata
     1621 attggacaga gcttccagct atgggatatg aagcggatga aggtactaaa agtcaagtgt
     1681 tttcaatttc aggagatgat ggtgtaaagc tatggaacat gattaatcct aataaaaaaa
     1741 ctgaagtcaa aagaaataat aaagaagatt ttaaagataa attggagcaa tactatccaa
     1801 ttgatatgga aagttttaat tccaacaaac cgaatgtagg tgacgaaaaa gagattgact
     1861 ttaagtttgc acctgacaca gacaaagaac tctataaaga agatatcatc gttccagcag
     1921 gatctacatc ttgggggcca agaatagatt tacttttaaa acccgatgtt tcagcacctg
     1981 gtaaaaatat taaatccacg cttaatgtta ttaatggcaa atcaacttat ggctatatgt
     2041 caggaactag tatggcgact ccaatcgtgg cagcttctac tgttttgatt agaccgaaat
     2101 taaaggaaat gcttgaaaga cctgtattga aaaatcttaa gggagatgac aaaatagatc
     2161 ttacaagtct tacaaaaatt gccctacaaa atactgcgcg acctatgatg gatgcaactt
     2221 cttggaaaga aaaaagtcaa tactttgcat cacctagaca acagggagca ggcctaatta
     2281 atgtggccaa tgctttgaga aatgaagttg tagcaacttt caaaaacact gattctaaag
     2341 gtttggtaaa ctcatatggt tccatttctc ttaaagaaat aaaaggtgat aaaaaatact
     2401 ttacaatcaa gcttcacaat acatcaaaca gacctttgac ttttaaagtt tcagcatcag
     2461 cgataactac agattctcta actgacagat taaaacttga tgaaacatat aaagatgaaa
     2521 aatctccaga tggtaagcaa attgttccag aaattcaccc agaaaaagtc aaaggagcaa
     2581 atatcacatt tgagcatgat actttcacta taggcgcaaa ttctagcttt gatttgaatg
     2641 cggttataaa tgttggagag gccaaaaaca aaaataaatt tgtagaatca tttattcatt
     2701 ttgagtcagt ggaagaaatg gaagctctaa actccaacgg gaagaaaata aacttccaac
     2761 cttctttgtc gatgcctcta atgggatttg ctgggaattg gaaccacgaa ccaatccttg
     2821 ataaatgggc ttgggaagaa gggtcaagat caaaaacact gggaggttat gatgatgatg
     2881 gtaaaccgaa aattccagga accttaaata agggaattgg tggagaacat ggtatagata
     2941 aatttaatcc agcaggagtt atacaaaata gaaaagataa aaatacaaca tccctggatc
     3001 aaaatccaga attatttgct ttcaataacc aagggatcaa cgctccatca tcaagtggtt
     3061 ctaagattgc taacatttat cctttagatt caaatggaaa tcctcaagat gctcaacttg
     3121 aaagaggatt aacaccttct ccacttgtat taagaagtgc agaagaagga ttgatttcaa
     3181 tagtaaatac aaataaagag ggagaaaatc aaagagactt aaaagtcatt tcgagagaac
     3241 actttattag aggaatttta aattctaaaa gcaatgatgc aaagggaatc aaatcatcta
     3301 aactaaaagt ttggggtgac ttgaagtggg atggactcat ctataatcct agaggtagag
     3361 aagaaaatgc accagaaagt aaggataatc aagatcctgc tactaagata agaggtcaat
     3421 ttgaaccgat tgcggaaggt caatatttct ataaatttaa atatagatta actaaagatt
     3481 acccatggca ggtttcctat attcctgtaa aaattgataa caccgcccct aagattgttt
     3541 cggttgattt ttcaaatcct gaaaaaatta agttgattac aaaggatact tatcataagg
     3601 taaaagatca gtataagaat gaaacgctat ttgcgagaga tcaaaaagaa catcctgaaa
     3661 aatttgacga gattgcgaac gaagtttggt atgctggcgc cgctcttgtt aatgaagatg
     3721 gagaggttga aaaaaatctt gaagtaactt acgcaggtga gggtcaagga agaaatagaa
     3781 aacttgacaa agacggaaat accatttatg aaattaaagg tgcgggagat ttaaggggaa
     3841 aaatcattga agtcattgca ttagatggtt ctagcaattt cacaaagatt catagaatta
     3901 aatttgctaa tcgggctgat gaaaatggga tgattcctta ttatctagta gatcctgatc
     3961 aagattcatc aaaatatcaa aagcttggcg agattgcaga atctaaattt aaaaatttag
     4021 gaaatggaaa agagggtagt ctaaaaaaag atacaactgg ggtagaacat catcaaggaa
     4081 atgaagaggc taccgaagaa aaatctagtt ttactattga tagaaatatt tcaacaatta
     4141 gagactttga aaataaagac ttaaagaaac tcattaaaaa gaaatttaga gaagttgagg
     4201 actttgtaac tggtggaaaa agaacagtag aatttgatta tcaatatgac gataaaggaa
     4261 atataactgc ctatgacgat ggtagtgcct tacaatatga aactgaaaaa cttgatgaaa
     4321 taaaatcaaa gatttatggt gttctaagcc cgtctaaaga tggacacttt gaaattcttg
     4381 gaaagataag taatgtttct aaaaatgcca aggtatatta tgccaataac tataaatcga
     4441 tagaaatcaa agcaactaag tatgactcac attcaaaaac gatgatattt gatttatacg
     4501 ctaatattaa tgatattgtg gatggattag cttttgctgg agatatgcgg ttttttgtta
     4561 aagatgatga tcgaataaaa gctgaaacta aaattagaat gcctgaaaaa aataaggaaa
     4621 ctaaagcaga atatccctat gtatcaagtt atgggaatgt aatcgaatta ggagaaggag
     4681 acctttcaaa aaacaaacca gacaatttaa ctaaaatgga atctggtaaa atctattctg
     4741 attcagaaaa acaacaatat ctgttaaagg ataacatcat tctaagaaaa ggctatgcac
     4801 taaaagtgac tacctataat cctggaaaaa cggatatgtt agaagggaat ggagtctata
     4861 gcaaggaaga tatagcaaaa atacaaaagg ccaatcctaa tctaagagtc ctttcagaaa
     4921 caacaattta tgctgatagt agaaatgttg aagatggaag aagtacccaa gctgtattaa
     4981 tgtcggcttt ggacggcttt aacattataa ggtatcaagt gtttacattt aaaatgaacg
     5041 ataaagggga agctatcgat aaagacggaa atcttgtgac agattcttct aaacttgtat
     5101 tatttggtaa ggatgataaa gaatacactg gagaggataa gtccaatgta gaagctataa
     5161 aagaagatgg ctccatgtta tttattgata ccaaaccagt aaacctttca atggataaga
     5221 actactttaa tccatctaaa tctaataaaa tttatgtacg aaatccagaa ttttatttaa
     5281 gaggtaagat ttctgataag ggtggtttta actgggaatt gagagttaat gaatcggttg
     5341 tagataatta tttaatctac ggagatttac acattgataa cactagagat tttaatatta
     5401 agctgaatgt taaagacggt gacatcatgg actggggaat gaaagactat aaagcaaacg
     5461 gatttccaga taaggtaaca gatatggatg gaaatgttta tcttcaaact ggctatagca
     5521 atttgaatgc taaagcagtt ggagtccact atcagttttt atatgataat gttaaacccg
     5581 aagtaaacat tgatcctaag ggaaatacta gtatcgaata tgctgatgga aaatctgtag
     5641 tctttaacat caatgataaa agaaataatg gattcgatgg tgagattcaa gaacaa
//
LOCUS       DAVGJL010000114         5665 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.115, whole genome shotgun sequence.
ACCESSION   DAVGJL010000114 DAVGJL010000000
VERSION     DAVGJL010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5665
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.115"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(330..1178)
                     /gene="dprA"
                     /locus_tag="VVE42_001747"
     CDS             complement(330..1178)
                     /gene="dprA"
                     /locus_tag="VVE42_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEU3073906.1"
                     /translation="MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLALSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPGSILDGLSDGCHHLIQEGAKLVTSGQDV
                     LAEFEF"
     gene            complement(1264..1953)
                     /locus_tag="VVE42_001748"
     CDS             complement(1264..1953)
                     /locus_tag="VVE42_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEU3073907.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVRLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQDIIVDSKAGRILSGVSFW
                     DAPTAEKIVSFIDKAYASGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     RKLEEILKNEN"
     gene            complement(1965..2843)
                     /locus_tag="VVE42_001749"
     CDS             complement(1965..2843)
                     /locus_tag="VVE42_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU3073908.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAATHD
                     FLSIFILLAFLLVKEGKVRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFVAVANGQL
                     LGLMIVFVAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAALFLGTSLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            complement(2833..3702)
                     /gene="licA"
                     /locus_tag="VVE42_001750"
     CDS             complement(2833..3702)
                     /gene="licA"
                     /locus_tag="VVE42_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HEU3073909.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTSI
                     KTKFDKIAPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLE
                     KRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGADFGYYGVNRYQRA
                     VKGLASYGGSDEK"
     gene            complement(3719..4741)
                     /locus_tag="VVE42_001751"
     CDS             complement(3719..4741)
                     /locus_tag="VVE42_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU3073910.1"
                     /translation="MINQIYQLTKPKFINVKYQEEAIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPESEIVVIGRHWEKLELFSFAKECYITDNI
                     PEDLAFDHAFECCGGDGTGPAINDLIHYIRPQGTILMMGVSEYKVNLNTRDALEKGLI
                     LVGSSRSGRIDFENAIQMMEVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            complement(4746..5453)
                     /locus_tag="VVE42_001752"
     CDS             complement(4746..5453)
                     /locus_tag="VVE42_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEU3073911.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITKGGADRNTSIKKIIEAIDAYRPL
                     TPEDIVVTHDSVRPFITLRMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPN
                     RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
BASE COUNT         1802 a         1180 c          963 g         1720 t
ORIGIN      
        1 tcactatact ttcccttata ctcaatgaaa atcaaagcgc aaactaggaa gctagccaca
       61 ggttgctcaa agcactgctt tgaagttgta gataagactg acgaagtcag ctcaaaatac
      121 tgttttgagg ttgcagatgg aagctgacgt agtttgaaga gattttcgaa gagtattata
      181 tgaaaaaatc ctctactcta ctaatccact tagaaaaaac gcatattatc aagtgttaaa
      241 aacttgctaa tatgcgtttt attatggaaa tgttgggttc attttctctt aaaattgatt
      301 ttttcttaga attctagctt aggtcatttt taaaattcaa attccgcaag aacatcttgc
      361 ccactggtga ccaattttgc tccttcttga atcaaatgat ggcaaccgtc tgatagtcca
      421 tctaaaatgc taccaggaat agcaaagaca tcgcgtcctt cttccattgc tcgctcacag
      481 gtaatgagac tacctgaacg catcttagcc tctgctacaa tcacaccacg acaaagtcca
      541 gcaatgatgc gattacgggc aggaaaatga aatttcagag gttgttcgcc aggtccatat
      601 tcacttagag ccagatggtc attgccgatg tagtcttgca agcgtttgtt ggctttagga
      661 taaaacacat ccagtcctgt tccaatcact gcaatggttt ttccgccatt ctgaagagct
      721 gccatatgag ctgctgtgtc aatgcccttg gccagaccac tgacaataac cagttcattt
      781 tccaagcctt gaatgacttt ttcaactgac ttagctccct gtttgctaca agcacgactg
      841 cccacgaccg ctaccttcgg gaatttcagg aggtcaagat ttcccttgta aaataaaagt
      901 acaggcgcat catatatttc actcaaatcc caaggataac agtcatctaa aatagagaaa
      961 gatggaaatt tttgaaactc tttcgacaaa tgcgcatcgt ctatctgaaa ataacgttcc
     1021 ataaaaacgg ctggattacg gcaacctgag atatctgcaa tatcacccaa caaaagctcc
     1081 tgatcaacat tttcaccgta ttctagcact ttcaaaatct gttgattggt caaacctgat
     1141 ttttttaact tatagatttc atagtttgtg attttcataa ataactccat ttctttttct
     1201 actcatctat ctattcgtaa aaaatcaaaa aacatccgac tattttgtca gatgttggaa
     1261 tctttaattt tcgtttttaa gaatttcttc taatttacga tagtcttgga cactatcgat
     1321 ctcataaatg ctattgcctt ctaattcttc aacatagaca tctagctctt tgatattatc
     1381 cttaaccata ttgtcccagt agagatcaac aaattcacca cttgcataag ccttgtcgat
     1441 aaagctgaca atcttttctg cagttggagc atcccagaag gatacaccac taaggatgcg
     1501 acctgccttg ctatcaacaa taatgtcttg aaccttgtag tcatctccat agaccaagaa
     1561 ccattcgttg gtacaatctt cacgataaac actaaaataa gtcgaacgtg tcaaatcatt
     1621 gcggaacata tttttaaaga gataattgtc agcatcaata acatagctgt tggccaattc
     1681 ttcttttaca agatagagag agtaaaagtt attgtagtca gcgtatttat cattgaaaac
     1741 gaggcgaaca ccgtattttt cttttaaata atcgaattgt tctttaagat aaccaacgat
     1801 gatgatgatg tcattgattc ctttttcttt gagaaactca atttggtatt caatcaaagg
     1861 tttttgatta acctgaacca aggctttagg ggtattttca gtcataggac gcaagcgagt
     1921 tcccaatccc gctgctaaga taatggcttt cacacgaatc tcctttattc tttaataata
     1981 atataaactc cagcaatgac gacaagtgac gtcataatgg tcagcatatc taatgatgtt
     2041 cccaagaaaa gagctgcgaa caagacagtc catactacat agctcacgtt caagcctgta
     2101 gccttggctg gttgcaagcg attgatagcg atataataag ccaaatagga aatcatatca
     2161 aaggctacaa agacaatcat gagacctagc aattgtccat tggctactgc aacaaatgac
     2221 tgatgagaga agagcacaat cacaagatag gacaagaacg aagttacttg acggattaag
     2281 agggcttcga tttcactcaa ttcactttcc atggcaaaag agctaagaac actctcactt
     2341 ccccatgcaa tagcacaaac caaagcacaa agaatcccaa tgtagaaaga attaacctgt
     2401 tcaaccttat aggtctgggc aataatccct ccaataatca agacaatccc aaatacagta
     2461 tttttcgaaa tcttgtgctt caaaaagaag aaagccaata gaactgaaat cgcagggtaa
     2521 atagccgata cagatgaagc taaagaactt ccgatatact taactgcata aagattggcc
     2581 tgcataccga tagggcctgc tagcaaggct ccgatgataa cactgacatt gcgaatattt
     2641 aagaaaattg agaggcgaac tttcccttct tttaccaaga gaaaagctag taagataaag
     2701 atgctcaaaa aatcatgagt tgcagccacc acaaagggtg acaaatctgt aaaaatcgaa
     2761 aagatataag cactaaccgt tagacccaag ccccagaaaa tacctgagag aaggccaaaa
     2821 ggaactccgt ttttactttt catctgaacc tccataagaa gccaaacctt taacagctct
     2881 ttggtaacga ttcacaccat agtaaccaaa atctgcacct tgctcttcct tatatacagt
     2941 ccatagactc caaatagtat cttgtaaaat tttataaata gcaatctttt catgagaaac
     3001 cggtgtttga tcactctcat agtgagataa gaaagtttct tcctcttggg aagtgaattc
     3061 agactctaaa aagagggcag ccaaatccca cattggatca ttcattgatg aatattccca
     3121 gtcaatcaaa taaagtcgtc cttgaggtga ttcgataaag ttttcaggca ccaaatcgat
     3181 atgacaagat tttctgtcaa cacctaagtc agccagtctt ttctctaagg agaagactgc
     3241 atttctaaca gattcatagt tggcataagg aatttgttct tcaatcaagg attcgtattt
     3301 tttgatttct tcaaaaggag caaattctcc tcttaattcc ttagcagatg tatgaatagt
     3361 ttgtaatatt ggagcaattt tgtcgaactt ggtcttgatt gacgttgaat caagcgtaat
     3421 cgcagattcg atatactcat ttactttgat accagcttca atatcaaaaa gataattttt
     3481 tacatctaag cctaaatcct ttagtagttc aagattgtac ttttcatctt gtcgattgat
     3541 aagcttttct gttcctttac caaagaattt aacaatgtat tgcttatttg ttgttttggc
     3601 caaatagttt tgattggtca ttccacccag ttgttcaaca ctgaggactt cctcttcttg
     3661 actaagtaag gaagaaattt tttctttaat gattttctcc acaattaacc tccagtactt
     3721 atacttccca cttaaacact gttttaaagg ctgtgtttaa atcggttgca aagacacggt
     3781 gaatatcttt aatttctctt acaggttctt ctagataaag gatattttta agacgattgg
     3841 caaatttctt gacttccatc atttggatag cattttcaaa atcaatgcga ccagaacgag
     3901 atgacccaac caaaatcaag cccttttcta aggcatcgcg agtattgaga ttgactttat
     3961 attcgctaac tcccatcatg agaatcgttc cctgaggacg aatgtagtga atcaagtcat
     4021 taatagctgg tccagtacca tcaccaccac aacattcaaa agcatggtca aaggccaaat
     4081 cttcaggaat attatccgta atatagcatt ctttggcaaa tgagaagagt tccaactttt
     4141 cccaatgacg accaataacc acaatctctg attctggcaa agtatagttg ataatattgg
     4201 caaccacaaa agctaaactt ccatctccaa taacggcaat ccgctcccgc ttgctatgag
     4261 caagagtcaa tagacgattc atagcgtgca tgcccacact gacaaactct gtaatggctg
     4321 caaccgtatc ttcaatagca tcataagcca ccacacgatc tttagggaga gaaacaaact
     4381 ctctcataaa gccatcaaat ccactagaca agaaatgggt ccctgtcatg tagttttcat
     4441 agaattcttc atcactctgc ataggagact gattgggaat catgacaact ttttgaccaa
     4501 cttcgtaggt tccggtcggg tcagaaatga cggttccaca tgactcgtga atcattgcca
     4561 ttggaagctt tttattcaaa atcttgggat cacgttttcc ctgatagtaa cgctgatccg
     4621 catgacagac agccatgtag ttgggacgga taaggatatg attctcttgg tcaatagcct
     4681 cttcctgata tttgacattg ataaacttag gcttagttag ttgataaatt tgattaatca
     4741 ttttactagt ctttctcaat catacttttt gcaatcttca aatctgttac ggttgtaatc
     4801 ttcagatttg agtattcacc tttggccaag gccacatctt ttcctttgat cacaaagatt
     4861 ttacatgcat ctgtcaagat ttccttctct tcatcagaaa gagatccata aaggtccatg
     4921 aagtccttgc aacggaatgt ttgaggtgtt tgtccttgat aaaggtgagc acgatttgga
     4981 atatctgtaa taaattgacc attggtactt tcaacgatag tatcaaccgc ttctaccact
     5041 gtgtccactg cgtcatgatt ttgggcaagt tggatattgt cctgaatcat gcgaagtgta
     5101 ataaatggac gaacagaatc gtgggtaaca acgatatcct ctggagtaag cggacgataa
     5161 gcatcaatgg cttcaatgat tttcttaata cttgtattgc ggtcagcacc accctttgta
     5221 atgatgatac gttccttata aagaggaaga tatttatcta caagatcttc tgcatgagaa
     5281 acccagtctc catgaacacc aactacaatt ttttcaatac ttggctccaa gacaaatttt
     5341 tcaattgtat gaatcaaaat aggtcgatca cctagctcta aaaattgttt tggcaagtta
     5401 ctgatcccca tgcgtgtgcc agttccaccg gcaagaattc ctgcataaat catctatttt
     5461 ctcctttgtc ttactaaaaa aacttattct ctctattata ccacaatcta gtcctatcat
     5521 aaaagaaata ctggtcctcc ccttctagaa taggagaatt atgcagttct attattcaaa
     5581 gaaactagtc catttctagt aatgactagg aattctgtag caattactaa taaatggctg
     5641 cgaaatttta gagtccttca gaata
//
LOCUS       DAVGJL010000115         5567 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.116, whole genome shotgun sequence.
ACCESSION   DAVGJL010000115 DAVGJL010000000
VERSION     DAVGJL010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5567
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.116"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            114..302
                     /locus_tag="VVE42_001753"
     CDS             114..302
                     /locus_tag="VVE42_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEU3073912.1"
                     /translation="MATDKNRIMISLDDKNLEKLENLVEDARDRRGMRLTKSQVIELL
                     LNTVDYFDDIMGAIYSKK"
     gene            364..1122
                     /locus_tag="VVE42_001754"
     CDS             364..1122
                     /locus_tag="VVE42_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU3073913.1"
                     /translation="MVVYIRQSKLPSEVSINKYNAQVGAYLQGEEAVLYQSFSEIKEL
                     TSEDIVVDYIMETRALLKMMGLNVPVHDYPIELKEFYGRKIYAGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNGTHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYGLS
                     PLKSINFHRARWKEMVKPYFEKNEIFKIQQDVVF"
     gene            1180..2034
                     /locus_tag="VVE42_001755"
     CDS             1180..2034
                     /locus_tag="VVE42_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEU3073914.1"
                     /translation="MLGAIIGDIVGSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMRISPC
                     AWIMDCGFYAKTGMWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCR
                     EYEQSINDNPTECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQETVPQAIIAFL
                     ESRDFEDAIRNAISLGGDSDTLAAITCSIAEAAYGIPDWIKDKAYSYLDEPLKDVVRR
                     WENRIKAY"
     gene            2146..3030
                     /locus_tag="VVE42_001756"
     CDS             2146..3030
                     /locus_tag="VVE42_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEU3073915.1"
                     /translation="MKKAMVIINPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILT
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEASHVLVLLTNYFADKKIFEENKDGYANIL
                     ILKDASIFSKLSVIPDLLKGDVIANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKPKED"
     gene            3193..4932
                     /locus_tag="VVE42_001757"
     CDS             3193..4932
                     /locus_tag="VVE42_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HEU3073916.1"
                     /translation="MELSAIYHRTESEYAYLYKDKKLHIRIRTKKGDIESINLHYGDP
                     FIFMEEFYQDTKEMVKITSGTLFDHWQVEVSVDFARIQYLFELRDTEGQNILYGDKGC
                     VENSLENLHAIGNGFKLPYLHEIDACKVPDWVSNTVWYQIFPERFANGNALLNPEGTL
                     DWDSSVTPKSDDFFGGDLQGIIDHMDYLQDLGITGLYLCPIFESTSNHKYNTTDYFEI
                     DRHFGDKETFRELVDQAHHRGMKVMLDAVFNHIGSQSLQWKNVVKNGEQSAYKDWFHI
                     QQFPVTTEKLVNKRDLPYHVFGFEDYMPKLNTANPEVKNYLLKVATYWIEEFNIDAWR
                     LDVANEIDHQFWKDFRKAVLAKNPDLYILGEVWHTSQPWLNGDEFHAVMNYPLSDSIK
                     DYFLRGIKKTDQFIDEINGESMYYKQQISEVMFNLLDSHDTERILWTANEDVQLVKSA
                     LAFLFLQKGTPCIYYGTELALTGGPDPDCRRCMPWERVSSDNDMLNFMKRLIKIRKYA
                     SVIISHGKYSLQEIKSDLVALEWKYEGRILKAIFNQSTEDYLLEKEAVALASNCQELE
                     NQLVISPDGFVIF"
     gene            5207..>5567
                     /locus_tag="VVE42_001758"
     CDS             5207..>5567
                     /locus_tag="VVE42_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073917.1"
                     /translation="MGAARDFLELINHKGLPLFNPLVGDSHVNNRQEYDNTGSGNLQP
                     EKWNETAKQLYNAIMWLIILWNAKPDTPLFNFKDEVIKYKTYEPFESSIKRVNTTIKN
                     GSKGKTLTEMINGYRADN"
BASE COUNT         1860 a          835 c         1185 g         1687 t
ORIGIN      
        1 aaagatttaa agttactgtg ttaaaaatcc tctccacttt taaactaatg tattataaaa
       61 gccagttgac acaagtgtta cacctgtgat agaattaact tgaggtgatg aaaatggcaa
      121 ccgataaaaa tagaataatg attagcttgg atgataaaaa tctagagaaa cttgaaaatt
      181 tagtagaaga tgctagagat agaaggggaa tgcgactaac aaagtcccag gttattgaat
      241 tactcctaaa taccgttgat tatttcgatg atattatggg ggctatttac tcaaaaaaat
      301 aagagcaaaa ttgctattat tttttacaaa ttggtgtaac actagtgata taggagtgat
      361 gaaatggttg tttatattag acaaagtaaa ttaccaagtg aggtttcaat taataaatac
      421 aatgctcaag taggtgccta cttacaagga gaagaagctg ttttatatca atctttttct
      481 gaaataaaag agttaacaag tgaagatata gttgtagatt atataatgga gactagagca
      541 ttactaaaaa tgatgggctt aaacgttccg gttcatgact atcctattga gcttaaagag
      601 ttttatggtc gaaagattta tgctggtatt ttaggagaga ttgtgaatat acctgataat
      661 tggggaaaat ttattaagcc taaagctggt tcaaaagtct ttactggaag agttgttaat
      721 ggaacccatg atttaatagg tattggtcta cctttcgact atcctatatg gattagtgag
      781 gttgtagaat tcatagctga atggcgttgt tttgtgttag atggtcgcgt attagatgtt
      841 cgaccctata caggtgatta tcatgcacaa tttgatgcaa gtgtaattga tgaagcgata
      901 tcatgttgga aagatgcgcc aatagcttat ggactagata tcggtgttac tcgcgatggc
      961 agaacgcttg ttgttgaagt aaatgatggt tatgcattgg gaaattatgg cctatctcct
     1021 ttaaaatcaa tcaattttca tagagctagg tggaaagaaa tggtaaaacc ctattttgaa
     1081 aaaaatgaaa tttttaaaat ccaacaggat gttgttttct aaaataaata tggtatataa
     1141 ccaatataaa aagcatcaaa atgaacaagg aggaatctga tgcttggagc aattattgga
     1201 gatattgtcg gttctgttta cgaatggaac aatatcaaaa caaaggactt tcctttattt
     1261 cggaaggact gcttttttac agatgacacg gttatgacct gtgctgttgc agaagcaatt
     1321 atgaatggtg gacaaaaaga cgatttcatt gacgcgatga agaaatatgg cagaatgtat
     1381 ccgaatgctg attacggtgc tcggtttaat gcatggctaa acagcgataa ccgtgagcct
     1441 tataatagct ttgggaatgg atcagctatg cgtatttctc catgtgcttg gatcatggac
     1501 tgtggttttt atgcgaaaac tggtatgtgg ccatcatcta gaggacttac gagtctttct
     1561 gcagaggtaa ctcataatca tccagaaggt gtcaaaggcg ctatggctac agctgatgct
     1621 atctttctat gtcgttttta ctttggtggt tattgtagag agtacgagca atcaattaac
     1681 gacaatccta cagagtgtaa aagacgaatt aaggattata tagaaaaaga atacgattac
     1741 aatctatctc aaactttaga tgaaatccgt cctaactatc gttttaacga aacatgtcag
     1801 gaaactgtcc ctcaagccat tatcgccttt cttgaaagta gagacttcga agatgcgata
     1861 agaaatgcca tctcacttgg tggcgacagt gatactctcg cagcaatcac ttgtagcata
     1921 gcagaggcag cttacggtat tcctgattgg atcaaggata aggcctattc ttacttggat
     1981 gaacccttaa aggatgtagt taggcgatgg gaaaatagaa taaaagcgta ttaatctcca
     2041 gtacgtcaga tttctctttt ctgtattttc attgtataat aggattacat ttacttgcat
     2101 acgacgaaat gtaaacattt ctgtttaaaa ttaaggataa aatcaatgaa aaaagcaatg
     2161 gtaattatca accctacttc tggtggcgag aaggctttgg attacaaaga aaagctggag
     2221 aataaagcaa aagaatactt tgaatatgtt gaaaccaaaa ttaccgaaaa agcgctggat
     2281 gcaacacatt ttgctgaaga agcttctcgt gagcagtatg atgcagtggt tgtgtttggt
     2341 ggagatggaa ctgtcaatga agtcatttca ggtattgatg agagagacta cattcctaag
     2401 ttagggatta tcccaggcgg tacgggtaac ctcattacaa aacttttgga aatcaatcaa
     2461 gacatcgatg gcgcaattga cgaactggat tttgatttaa ccaataagat tgatatcggt
     2521 aaagcaaatg acaactattt tggttatatc tttagtatcg gttctctgcc tgaggcgatt
     2581 cacaatgttg aaatagagga caaaacaaaa ttcggtattt taacctatgc tgtaaatacc
     2641 atgaagtctg tcatgacaga tcaggtcttt aacattaagg ttgagacaga aaatggaaat
     2701 tatgttggtg aagctagcca tgttttggtc cttttgacaa attacttcgc tgataagaaa
     2761 atctttgaag aaaacaagga cggctatgcc aacattttga ttctgaaaga tgcctctata
     2821 ttctccaaat tatccgtcat tcctgattta ttaaaagggg atgttatcgc aaatgataat
     2881 atcgagtata tcaaagcgcg taatattaaa atctcttcag atagtgaatt ggagtcagat
     2941 gttgacggag ataaatcaga taacctacct gtagaaatca aagtcctagc tcagcgagta
     3001 gaagtatttt caaaaccgaa agaggattag tatatagaga aagccttttt taaggctttt
     3061 tgtatacttt aaaagatagt tcctttaaca acggacattc cttgcaaata gttcacaaaa
     3121 atagtatact ggattcattg agtttgaaaa cgtttgcgta aaatttgaat gaatacttta
     3181 ggagataaat tgatggaatt gagtgctatt taccatagga ctgagtcgga gtatgcctat
     3241 ctttataagg ataagaaact ccatattcga attcgaacta agaaagggga cattgaaagc
     3301 atcaacttgc actatgggga cccttttatc tttatggagg agttttatca ggatacaaaa
     3361 gaaatggtca agataacttc tggtacctta tttgaccatt ggcaggttga agtgtcagtt
     3421 gactttgcac gtatccagta tctttttgag ctcagagata cagaaggtca aaatattttg
     3481 tatggcgata aagggtgtgt ggaaaattct ctagaaaatc ttcatgcaat tgggaatgga
     3541 tttaagttgc cttatcttca tgagattgat gcctgcaagg ttcctgactg ggtttcaaat
     3601 acggtatggt atcagatatt tcctgaaaga tttgccaatg gcaatgctct attaaaccca
     3661 gaagggactt tagactggga ttcatctgtc acacctaaga gcgatgattt ctttggtggt
     3721 gatttacagg ggattattga tcatatggat tacttgcaag acttgggtat tactggacta
     3781 tatctttgtc ccatctttga atctacaagc aatcacaagt acaatacgac agattacttt
     3841 gaaattgacc gtcattttgg agacaaggag acctttcggg aactggtgga tcaagcgcat
     3901 catcgtggca tgaaagtcat gctggatgcg gtatttaatc atattggttc gcaatctctt
     3961 caatggaaaa atgtcgtcaa aaatggtgaa cagtctgctt ataaggattg gttccatatt
     4021 caacaattcc cagtgacaac tgaaaagcta gttaataaga gagacttacc ctatcatgtt
     4081 tttggtttcg aggactatat gcctaagcta aatacagcca atccagaggt caagaattat
     4141 cttttaaagg ttgcgactta ttggattgaa gagtttaata tcgatgcttg gcgtttggat
     4201 gtggctaatg agattgacca tcagttctgg aaggattttc gtaaggcagt tttagctaaa
     4261 aatcctgatc tttatatcct aggagaagtc tggcatacat ctcagccttg gctaaatgga
     4321 gatgagttcc atgccgtcat gaattatcct ttatctgata gtatcaagga ctatttctta
     4381 cgaggaatta agaagacaga ccagttcatc gatgaaatca atggagagtc tatgtattac
     4441 aagcagcaga tttcagaggt catgtttaat ctcttggatt cacatgatac agagcgaatc
     4501 ctgtggacgg ccaatgaaga tgttcaactg gttaaatcag ccttagcctt tctcttttta
     4561 caaaaaggaa caccgtgcat ttattacgga accgagctag ccttgactgg aggaccagat
     4621 ccagattgtc gtcgttgtat gccttgggaa cgtgtatcaa gtgacaatga tatgctgaac
     4681 tttatgaaga ggctgattaa gattcggaaa tacgcgtcag taatcatttc gcatggcaag
     4741 tatagccttc aagaaatcaa atctgatcta gtagctctgg aatggaaata cgaaggacgg
     4801 atcctcaaag caatattcaa ccaatcaaca gaagattatc ttttagagaa agaagcagta
     4861 gcactagcaa gcaattgcca agaattggag aatcagcttg tcatctctcc agatggattt
     4921 gtgattttct aaaaactagt tgatgaagat tatggtacat ttcatatctt atatagtata
     4981 ataaggctag ttactaaact tgtaaaggag aacttaaatg aattgtagag gacatgaaac
     5041 aagacaaaga attgttagag attttgaagt ttagcctaaa gcacatatta agctgttagc
     5101 aaatcaacaa aaacatagtg atgcaggagc aactattgaa gatgaatatt atgtatttat
     5161 cgctgagagt aaaattgatg gcaagaagga agttattcag tgttgcatgg gtgcggcaag
     5221 ggatttttta gaactaatta atcacaaagg gctacctctt tttaatccgc ttgtaggtga
     5281 ttctcatgta aataatagac aagaatatga caatacaggg agtggaaatt tacaacctga
     5341 aaagtggaat gaaactgcaa agcagcttta taatgctata atgtggttga ttattttatg
     5401 gaatgctaag ccggatacac ctttatttaa ttttaaagac gaagtaatta agtataaaac
     5461 atatgagcct tttgaaagca gtataaaaag agtaaatact actataaaga atggtagtaa
     5521 agggaaaact ctgactgaga tgattaatgg ctacagagcg gataacg
//
LOCUS       DAVGJL010000116         5323 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.117, whole genome shotgun sequence.
ACCESSION   DAVGJL010000116 DAVGJL010000000
VERSION     DAVGJL010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5323
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.117"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            81..1196
                     /gene="ychF"
                     /locus_tag="VVE42_001759"
     CDS             81..1196
                     /gene="ychF"
                     /locus_tag="VVE42_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEU3073918.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELDDEDKKEFLEVIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQAAGIIHSDFEKGFIRAVTMSYED
                     LVKYGSEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            1267..1836
                     /locus_tag="VVE42_001760"
     CDS             1267..1836
                     /locus_tag="VVE42_001760"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEU3073919.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     QADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTQVFNRVKIGIGRPKNGMSVVHHVLSKFDRDDYIGI
                     LQSIDKVDDSVNYYLQEKNFEKTMQRYNG"
     gene            1837..>5323
                     /gene="mfd"
                     /locus_tag="VVE42_001761"
     CDS             1837..>5323
                     /gene="mfd"
                     /locus_tag="VVE42_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEU3073920.1"
                     /translation="MVTLLDLFSENDQIKKWHQNLTDKKRQLILGLSTSTKALAIASS
                     LEKEDRIVLLTSTYGEAEGLVSDLISILGEELIYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIVASRLILPSPNAFKDSIVKISVGEEYDQHAFIHQLKENG
                     YRKVTQVQTQGEFSLRGDILDIFEISQLEPCRIEFFGDEIDGIRSFEVETQLSKENKT
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERDLAQYFTEELQNSKAFSDM
                     QYFSDIEQIYKKQSPVTFFSNLQKGLGNLKFDKIYQFNQYPMQEFFNQFSFLKEEIER
                     YKKMDYTIILQSSNSMGSKTLEDMLEEYQIKLDSRDKTNICKESVNLIEGNLRHGFHF
                     VDEKILLITEHEIFQKKLKRRFRRQHVSNAERLKDYNELEKGDYVVHHIHGIGQYLGI
                     ETIEIKGIHRDYVSVQYQNGDQISIPVEQIHLLSKYISSDGKAPKLNKLNDGHFKKAK
                     QKVKNQVEDIADDLIKLYSERSQLKGFAFSADDDDQDAFDDAFPYVETDDQLRSIEEI
                     KRDMQASQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVVILVPTTVLAQQHYTNFK
                     ERFQNFAVNVDVLSRFRSKKEQTATLEKLKNGQVDILIGTHRVLSKDVVFADLGLMII
                     DEEQRFGVKHKETLKELKKQVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPV
                     QTYVLEKNDSVIRDAVLREMERGGQVYYLYNKVDTIVQKVSELQELIPEASIGYVHGR
                     MSEVQLENTLLDFIEGQYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQLRGR
                     VGRSNRIAYAYLMYRPEKSISEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNLLG
                     KSQSGFIDSVGFELYSQLLEEAIAKRNGNANANTRTKGNAELILQIDAYLPDTYISDQ
                     RYKIEIYKKIRQIDNRVNYEELQEELIDRFGEYPDVVAYLLEIGLVKSYLDKVFVQRV
                     ERKDNKITIQFEKVTQRLFLAQDYFKALSVTNLKAGIAENKGLMELVFDVQNKKDYEI
                     LEGLLIFGESLLEIKE"
BASE COUNT         1801 a          788 c         1115 g         1619 t
ORIGIN      
        1 ctatatcaag gaattttagc aagtttaccc tttttgtgtt ataatattag ggattgaaat
       61 gaaaacggag aatgagaaat atggctttga cagcaggtat cgttggtttg ccaaacgttg
      121 gtaaatcaac actatttaat gcaattacaa aagcaggagc agaggcagca aactacccat
      181 ttgcgactat tgatccaaat gttggaatgg tggaagttcc agatgaacgc ctacaaaaac
      241 taactgaaat gataactcct aaaaagacag ttcccacaac atttgaattt acggatattg
      301 cagggattgt aaaaggagct tcaaaaggag aagggctagg gaataaattc ttggccaata
      361 ttcgtgaagt agatgcgatt gttcacgtag ttcgtgcttt tgatgatgaa aatgtgatgc
      421 gcgagcaagg acgtgaagac gcctttgtag atccacttgc agatattgat acaattaatc
      481 tggaattaat tcttgctgac ttagaatcag tgaacaaacg atatgcgcgt gtagaaaaga
      541 tggcacgtac gcaaaaagat aaagaatcag tagcagaatt caatgttctt caaaagatta
      601 aaccagtcct agaagacggg aaatcagctc gtaccattga atttacagat gaggaacaaa
      661 aggttgtcaa aggtcttttc cttttgacga ctaaaccagt tctttatgta gctaatgtgg
      721 acgaggatgt ggtttcagaa cctgactcta tcgactatgt caaacaaatt cgtgaatttg
      781 cagcgacaga aaatgctgaa gtagtcgtta tttctgcgcg tgctgaggaa gaaatttctg
      841 aattggatga tgaagataaa aaagagtttc ttgaagtcat tggtttgaca gaatcaggtg
      901 tagataagtt gacgcgtgca gcttaccact tgcttggatt gggaacttac ttcacagctg
      961 gtgaaaaaga agttcgcgct tggactttca aacgtggtat gaaggctcct caagcagctg
     1021 gtattatcca ctcagacttt gaaaaaggct ttattcgtgc agtaaccatg tcatatgaag
     1081 atctagtgaa atacggatct gaaaaggccg taaaagaagc tggacgcttg cgtgaagaag
     1141 gaaaagaata tatcgttcaa gatggcgata tcatggaatt ccgctttaat gtctaaaaat
     1201 taataaatgg tgtcaattag gttggaaaaa aattccaacc cttttggctt ttgaaaggaa
     1261 aaataaatga ccaaattact tgtaggcttg ggaaatccag gggataaata ttttgaaaca
     1321 aaacacaatg ttggttttat gttgattgat caactagcga agaaacagaa tgtcactttt
     1381 acacacgata agatatttca agctgaccta gcatcctttt tcctaaatgg agaaaaaatt
     1441 tatctggtta aaccaacgac ctttatgaat gaaagtggaa aagcagttca tgctttatta
     1501 acttactatg gtttggatat tgacgattta cttatcattt acgatgatct tgacatggaa
     1561 gttgggaaaa ttcgtttaag agcaaaaggc tcagcaggtg gtcataatgg tatcaagtct
     1621 attattcaac atataggaac tcaggtcttt aaccgtgtta agattggaat tggaagacct
     1681 aaaaatggta tgtcagttgt tcatcatgtt ttgagtaagt ttgacaggga tgattatatc
     1741 ggtattttac agtctattga caaagttgac gattctgtaa actactattt acaagagaaa
     1801 aattttgaga aaacaatgca gaggtataac ggataaatgg tgaccttatt agatttattc
     1861 tcagaaaatg atcagattaa aaaatggcat caaaatttaa cagataagaa aagacaacta
     1921 atacttggtt tatcaacatc tactaaggct cttgcaattg caagcagttt agaaaaagaa
     1981 gataggattg tgttattgac gtcaacttat ggagaagcag aaggacttgt tagtgatctt
     2041 atatctatct tgggtgagga actcatctat ccatttttgg tagatgatgc tcctatggtg
     2101 gagtttttga tgtcttcaca ggaaaaaatt atttcacggg ttgaagcctt gcgttttttg
     2161 actgattcat ctaagaaagg gattttagtt tgtaatatcg tagcaagtcg attgatttta
     2221 ccgtctccca atgcattcaa agatagtatt gtaaaaatct cagttggtga agaatatgat
     2281 caacacgcgt ttatccatca gttaaaggaa aatggctatc gaaaagttac tcaagtacaa
     2341 actcagggcg aatttagtct tcgaggagat attttagata tttttgaaat atcccagtta
     2401 gaaccttgtc gaattgagtt ttttggtgat gaaattgatg gtatcaggtc atttgaagta
     2461 gaaacacaat tatcgaaaga aaataagaca gaactcacta tctttccagc tagtgatatg
     2521 cttttgagag aaaaggatta tcaacgagga cagtcagctt tagaaaaaca aatttcaaaa
     2581 actttatcac ctattttgaa atcataccta gaagaaattc tttcaagttt tcaccaaaaa
     2641 caaagtcatg cagactctcg gaagttttta tctttgtgct atgataagac atggactgtc
     2701 tttgattata ttgaaaaaga taccccaata ttctttgatg attatcaaaa attgatgaat
     2761 cagtatgaag tctttgaaag agacttagcg cagtacttta cagaagaatt acagaatagt
     2821 aaagcatttt ctgatatgca gtatttttct gatattgaac aaatctataa aaaacaaagt
     2881 ccagtgacct ttttctctaa tcttcaaaag ggtttaggaa atctcaaatt tgacaaaatt
     2941 tatcaattca atcaatatcc tatgcaggaa tttttcaatc agttttcttt tctaaaagaa
     3001 gaaattgaac gatataaaaa aatggattac accattattc tgcagtctag caattcaatg
     3061 ggaagtaaaa cattggagga tatgttagag gaatatcaga ttaaattgga ttctagagat
     3121 aagacaaata tctgtaaaga atctgtaaac ttaatagagg gtaatctcag acatggtttt
     3181 cattttgtag atgaaaagat tttattgata actgaacatg agatttttca aaagaaatta
     3241 aagcgtcgtt ttcgaagaca acatgtttca aatgcagaga gattaaaaga ttacaatgaa
     3301 cttgaaaaag gggactatgt tgtccatcat atccatggga ttggtcaata tctaggaatt
     3361 gaaaccattg aaatcaaggg aattcatcgc gattatgtca gtgtccaata ccaaaatggt
     3421 gatcaaattt ctatccccgt ggaacagatt catctactgt ccaaatatat ttcaagtgat
     3481 ggtaaagctc caaaactcaa taaattaaat gacggtcatt ttaaaaaggc caagcaaaag
     3541 gttaagaacc aggtagagga tatagctgat gatttaatca aactctactc tgaacgtagt
     3601 cagttgaagg gttttgcttt ctcagctgat gatgatgatc aagatgcctt tgatgatgct
     3661 ttcccttatg ttgaaacgga tgatcaactt cgtagtattg aggaaatcaa gagggatatg
     3721 caggcttctc agccaatgga tcgactttta gttggggatg ttggttttgg aaagactgaa
     3781 gttgctatgc gtgcagcctt taaagcagtc aatgatcaca aacaggttgt cattctagtt
     3841 ccgacgacgg ttttagcgca acagcactat acgaatttta aggaacgatt ccaaaatttt
     3901 gcagttaatg ttgatgtgtt gagtcgcttt agaagtaaaa aagagcagac tgcaacactt
     3961 gaaaaattga aaaacggtca agtcgatatt ttgattggaa cacatcgtgt tttgtcaaaa
     4021 gatgttgtgt ttgctgattt gggcttgatg attattgatg aggaacagcg atttggtgtc
     4081 aagcataagg aaactttgaa agaactgaag aaacaagtgg atgtcctaac cttgaccgct
     4141 acgccaatcc ctcgtaccct ccatatgtct atgctgggaa tcagagattt gtctgttatt
     4201 gaaactccgc cgactaatcg ctatcctgtt cagacctatg ttttggaaaa gaatgatagt
     4261 gtcattcgtg atgctgtctt gcgtgaaatg gagcgtggag gtcaagttta ctatctttac
     4321 aacaaagttg acacaattgt tcagaaggtt tcagaattac aggagttgat tccggaggct
     4381 tcgattggat atgttcatgg tcgaatgagt gaagtccagt tggaaaatac tctattagac
     4441 tttattgagg gacaatacga tatcttggtg acgactacta ttattgagac aggggtggac
     4501 attccaaatg ctaatacttt atttattgaa aatgcggacc atatgggctt gtcaacctta
     4561 tatcagttaa gaggaagagt cggtcgtagt aatcgtattg cttatgctta tctcatgtat
     4621 cgtccagaaa aatcaatcag tgaagtctct gaaaagagat tagaagcgat taaaggattt
     4681 acagaattgg gctctggctt taagattgca atgcgagatc tttcgattcg tggagcagga
     4741 aatcttttag gaaaatccca gtctggtttc attgattctg ttggttttga attgtattcg
     4801 cagttattag aggaagctat tgctaaacga aacggtaatg ctaacgctaa cacaagaacc
     4861 aaagggaatg ctgagttgat tttgcaaatt gatgcctatc ttcctgatac ttatatttct
     4921 gatcaacgat ataagattga aatttacaag aaaattcgtc aaattgacaa ccgtgtcaat
     4981 tatgaagagt tacaagagga gttgatagac cgttttggag aatacccaga tgtagtagcc
     5041 tatcttttag agattggttt ggtcaaatca tacttggaca aggtctttgt tcaacgtgtg
     5101 gaaagaaaag ataataaaat tacaattcaa tttgaaaaag tcactcaacg actgttttta
     5161 gctcaagatt attttaaagc tttatccgta acgaacttaa aagcaggcat cgctgagaat
     5221 aagggattaa tggagcttgt atttgatgtc caaaataaga aagattatga aattttagaa
     5281 ggtctgctga tttttggaga aagtttatta gagataaaag agt
//
LOCUS       DAVGJL010000117         5256 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.118, whole genome shotgun sequence.
ACCESSION   DAVGJL010000117 DAVGJL010000000
VERSION     DAVGJL010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5256
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.118"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            325..684
                     /locus_tag="VVE42_001762"
                     /pseudo
     CDS             325..684
                     /locus_tag="VVE42_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379612.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            694..1059
                     /locus_tag="VVE42_001763"
     CDS             694..1059
                     /locus_tag="VVE42_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEU3073921.1"
                     /translation="MVYRYRTNLKKVFLTDSELHQLNERIDKSHCQNFSVYARKVLLN
                     PNMSFVTINTDTYDQLVFELRRIGNNINQIARAINQSRLISQEQLQELSKGVGELIKE
                     VDKEFQVEVKRLKEFHGSH"
     gene            1046..2896
                     /locus_tag="VVE42_001764"
     CDS             1046..2896
                     /locus_tag="VVE42_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEU3073922.1"
                     /translation="MVVTKHFATHGKKYRRRLIKYILNPDKTDNLKLVSDFGMSNYLD
                     FPSYEEMVEMYNVNFTNNDKLYEYRNDRQEKHQQNIHAHHLIQSFSPEDNLTPEEINR
                     IGYETIMELTGGRFRFIVATHTDKDHIHNHILINAIDCNSDKKLIWNYALERNLRMIS
                     DRISKMAGAKIIEKRFSYRDYQKYRATSHKFELKQRLYFLMQQSKSFDDFLEKAEQLH
                     VHIDFSQKHSRFMMTDRAMTKPIRGRQLSKRDLYDEDFFRMHFAKQEIASRLEFLLNC
                     VNSLEGLLTKSKELNLTIDLKQKNVIFILEENGKQFSLSHKKISDEKLYDVNFFQDYF
                     KNKEVGVSEGIENLQAQYRAFQEERDKEKVSTEEIEEAFETFKEKRDAVHEFEVKLTE
                     HQIEKLVDEGIYIKVSFGINQSGLIFIPNYQLDIMEEENQKKYKVYIRETTSYFVYNK
                     EHSDKNQYIKGRTLIRQLTNDSRVIPYRRPTVERLQEKISEISLLIELTETDKKYQDI
                     KDNLVSEIAELDIKLTQTNEKIATLNKMAEVLINSKSEGSGSQKLARHEFSKLNMTES
                     TTLEQVNEELLKLQQEFGNVLDEYEKTIRKLGQLFKVFDECINKEIMNEI"
     gene            3152..4584
                     /locus_tag="VVE42_001765"
                     /pseudo
     CDS             3152..4584
                     /locus_tag="VVE42_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886556.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4822..4956)
                     /locus_tag="VVE42_001766"
                     /pseudo
     CDS             complement(4822..4956)
                     /locus_tag="VVE42_001766"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
BASE COUNT         1793 a          871 c         1029 g         1563 t
ORIGIN      
        1 ttgttatgag agcttttgct ctcttttttc ttaccttcct ttcaataatg tgataccttt
       61 tccaaatcat ccctgttgtc ctaatatcat tcctaagaaa ataaaacctc aatttttcac
      121 tgggggtttg ggggcgaagc caccaagtta tcttatggtt agctgtcaaa actggaaggt
      181 tttggttagc tggcgatatg attttttggg tattgtgacc acaatgcctg agctcgcaaa
      241 gaccgaacaa ggactttaag aaatttcccg tatgacacac ggggtcgggg tttacccgag
      301 aaaggatgaa agtgaggaaa agagatgtct gaacagtacc gtgacattcg aaaagaggta
      361 aatctaaccg cagatgagct aaaacagatt gaaaaaatga tggaggttga taattatcgt
      421 cacttttcat cattcgtcag ggataaaatt ttgatgactg atgacaaaca gttagctgcc
      481 aaagaatgat tttccctttg gcagtctcaa aagtttgaac agatcagtcg agatgtgcat
      541 ttggttttga ttatcgcaag agagaatcac caagtgactc aagaacacgt gtcaatctta
      601 ttgacctgcg ttcaagaatt gattgcggaa gtcaatcaag tacagtcact cagtcgtggg
      661 tttcgtgaaa aatacatgag ataggaggct actatggttt atcgttatcg taccaatctc
      721 aaaaaagtat ttctaacaga ttcagaatta catcaattga atgagcgaat cgataagagt
      781 cactgtcaaa atttttcagt ctatgccagg aaagtgttgc tgaatcccaa tatgtcattt
      841 gtcaccatta atacggatac ctatgaccag ttagtatttg aattgagacg gatcggaaat
      901 aacattaatc aaattgcgcg tgctatcaat caaagccgtc tgatttctca ggaacagtta
      961 caagaattga gtaaaggagt cggagagtta attaaggaag tggacaaaga atttcaagtg
     1021 gaagtgaaaa gattgaagga gtttcatggt agtcactaag cattttgcga cacacggaaa
     1081 aaaatatcgt aggcgtttaa ttaagtatat cctcaatcct gataaaacgg acaatttgaa
     1141 attagtatct gattttggta tgagcaatta cttagacttt cctagctatg aagaaatggt
     1201 agaaatgtac aatgtcaact ttaccaataa tgacaagttg tacgaataca gaaatgaccg
     1261 acaagaaaaa caccaacaaa atattcatgc ccatcacctc attcaatcat tttctcctga
     1321 agataatctg acacctgaag aaattaaccg cattggttat gagaccatta tggaattaac
     1381 aggaggccgt tttcgtttca tcgtagcgac tcatacagac aaagatcata ttcataatca
     1441 catcttaatc aacgccattg actgtaattc agataaaaag ttgatatgga attatgcctt
     1501 ggaacgaaac cttcgtatga tttcagaccg catttctaaa atggctgggg cgaaaattat
     1561 tgaaaagcgt ttctcttacc gtgactatca aaaatataga gcgactagcc ataagtttga
     1621 actaaagcaa cgtctttatt ttttgatgca gcagtcaaaa tcctttgatg attttttaga
     1681 aaaagcagag caattacatg ttcatattga ttttagtcag aagcatagtc gattcatgat
     1741 gacggatcgg gcaatgacaa aaccaattcg aggacgtcaa ctcagtaaac gagatttata
     1801 tgatgaagat ttttttagaa tgcattttgc caagcaagag attgcaagtc gtttagaatt
     1861 tttgttgaac tgtgtcaatt ctttagaagg gttactaaca aaatcaaaag aattgaatct
     1921 aaccattgac ttaaaacaaa aaaatgtaat ctttatactt gaagaaaatg gaaagcaatt
     1981 cagtctgagt cataaaaaaa taagtgatga gaaattatat gatgtcaatt tttttcaaga
     2041 ttattttaaa aataaggaag taggtgtttc agaaggaata gaaaatctac aggcacagta
     2101 ccgtgctttt caagaagaac gagataagga gaaggtatct actgaagaaa ttgaggaagc
     2161 ctttgagaca tttaaggaaa aacgagatgc cgttcatgaa tttgaagtaa aacttacaga
     2221 acaccaaatt gagaagttag ttgatgaggg gatttatatc aaagtgtcct ttggtataaa
     2281 tcagagtggt cttattttca ttcctaacta tcaactggat attatggaag aagaaaatca
     2341 gaaaaaatat aaagtctaca ttcgtgagac aacctcatat tttgtctata acaaagaaca
     2401 ttcggataag aatcagtata tcaaagggag aaccttgatt aggcagctaa ccaacgatag
     2461 tcgagtaata ccatacagaa gaccgactgt tgaaagactt caagaaaaga tttctgagat
     2521 tagcctcttg attgagttaa ctgagacaga taagaaatac caagacatta aagacaactt
     2581 ggtttccgaa atagcagagc tagatataaa actgactcaa acaaatgaaa aaattgccac
     2641 cttaaataag atggcggaag tacttatcaa ttctaagagt gaaggttcgg gtagccaaaa
     2701 actagctagg catgaatttt ctaaattgaa catgacagaa tcaacaactt tagaacaagt
     2761 caatgaagaa cttttgaagt tacaacagga atttggtaat gttcttgatg agtatgagaa
     2821 aacaataaga aagcttggac aattatttaa ggtttttgat gagtgtatca ataaagaaat
     2881 tatgaatgaa atataaatca gactgaagac aaactcaaat ttttttggat atcttttctg
     2941 tttatggttg ccaatatcgg tttaacttct aaaacaaaca ttaataaaaa tgcattcaaa
     3001 gacgatttta cctattttac gattacgctt cgaccccaaa tcattaaagt tgtctatgct
     3061 ctatcattgc atcactggac ttgtcaagag ttaagataat tcggagtcca ctgaaaaagt
     3121 catcgtatga tggtatacta aagatgaggt gatggtatgc ttgataatcg atgttagtcc
     3181 ctattctact ctgtgtgata ttgttgttcc caaaactcat tttttacgac agctaatgga
     3241 actctgtgat tttagtttta tttatgacga gctggaaaag aattatcaac ctgacttcgg
     3301 atgtcgttcc tactctctcc ttatcatgat gttcaagtat ctcttattaa aagacatcta
     3361 taagttgtcc gatgtggatg tggtggagcg ctccttttct ggtatgacct tcaaatactt
     3421 ccttggtcta gctcctgtta ttgaaccgtc ctcattgact aaattccgaa aacttcggaa
     3481 caaagatgaa cgattacttg atttgctcat cgctaaatct gttcagattg cgatagagct
     3541 cggtctgatt aaaagtaaca ttctcattgt ggatgcgact cataccaaag ttcattacaa
     3601 tcacaagaaa cctcaagaag tcctccgaga acgctcaaaa gccttgcgta aaacgattta
     3661 tcagtattcg gaatatatca aggcagaatt tcctagtaag cctcaggaag atactctagt
     3721 agctgaatta agatatacac aagaagtaat atcagtcctt gagaagcatg acgaattgac
     3781 tggaatacca gctatctcac aaaattcaat taccttaaag aagctgtaga ggatgactta
     3841 gagcacttgg aatcctctgt caaagaagaa gcaagaattg gacataagtc agccgatagt
     3901 tccttttacg ggtacaaaga gcattttgcc atgaccgatg aacggattat tactgcctgt
     3961 gtggtgacat cgggtgaaaa aagtgatggt cctgttttag aagaacttta tcacaaatct
     4021 aaggataatg gcgtgacgat tgaagctatt gttggagata gggcctactc tgggaaggat
     4081 aatatgcaat ttaccaagaa agaacgtgtg cattgagtcg ctaggccata tccaactatt
     4141 tcaaaaggct ttcgtaggaa ggaagatgag tttttctata acaaggacgc aggtctcttt
     4201 gtttgtccag aagggcatat ggcactaaag aaggcaaaga caggaagaag gacggctcag
     4261 gaaacccact attttgacat caagcactgt caaatatgcc cttcaaaagt gggcagttat
     4321 aaagaagggg ctaagtctaa gacttatttg atacccctta agagcgatga acatattttc
     4381 cagaagaggt ttcaggaagc gccctatttt aaagaaaagg ccaaacatcg ctacaagata
     4441 gaagccaaaa atgcggaact tgaaaacaga catggtctta atgtagctag agcaccaggt
     4501 cttttaaaca tggagttaca agccgccaca accatatttg tggttaatat gaagaggatt
     4561 ataactctaa taaaccaaaa ataaggaaaa gcaaagagga atctatccaa gcaggagata
     4621 gatccctctt ttttcatttt agttgaactg tagagcttac tttttcagtg gactcgatga
     4681 ttagtgacta tttttttcaa actctattag ttattggtac cctaatgccg aatggagata
     4741 tttaggattg taatagattt caatatattt aaagatttcg ttcaatagtt gcgaaattgg
     4801 catcattgac aagttctctt tttatgtttt gtaaaaggat tccatgggtg cattatcata
     4861 tggattgcct tttcgactca ggctagactt gcatcttttc tgtttaagga gttcttgaaa
     4921 tctagctcca gtatattgag atccttgatc ggtatggccg actagttctt atttgagatt
     4981 ttctctatga taagcttgat tgaaaacatt cgttaccaac ttatcttgca ttcgatttcc
     5041 cattgcctaa ccaacaattt ttctgctata aacatatata taatgataaa taaagagtac
     5101 ctgcttttgt cggaatataa gttaaatccc ccagccagat ttcattttta ccttttgttt
     5161 gaaaacactg attaatcaaa ttgggtcgtg ttaatgagga atgccgacgg ctatagtact
     5221 tgtatttata gcggattcct ttagcataga gacaaa
//
LOCUS       DAVGJL010000118         5221 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.119, whole genome shotgun sequence.
ACCESSION   DAVGJL010000118 DAVGJL010000000
VERSION     DAVGJL010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5221
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.119"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(778..1647)
                     /locus_tag="VVE42_001767"
     CDS             complement(778..1647)
                     /locus_tag="VVE42_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEU3073923.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTENPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKAIEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIVA
                     AIAAVAVFKFLYL"
     gene            complement(1871..2392)
                     /locus_tag="VVE42_001768"
     CDS             complement(1871..2392)
                     /locus_tag="VVE42_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU3073924.1"
                     /translation="MVPKTATSTETKTITRIIHYVDKVTNQNVKEDVVQPVTLSRTKT
                     ENKVTGVVTYGEWTTGNWDEVVSGKIDKYKDPDIPTVESQEVTSDSSDKEITVRYDRL
                     STPDKPTPDPGTPKTETPVNPDPEVPTYETGKREELPNTGTEANATLASAGIMTLLAS
                     LGLGFFKKKEDEK"
     gene            complement(2350..3456)
                     /locus_tag="VVE42_001769"
     CDS             complement(2350..3456)
                     /locus_tag="VVE42_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEU3073925.1"
                     /translation="MNIILIAKLLRENTNTKANALNNGWARSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVGSGDSKLGKGGYFSTGDVLLGKDVVSYTVQVFSENNE
                     RVGVNTQSHRVQYNLPILADFSVIQDTVEPSRTVVEKIIPKLNIPEEEKGKITEEIKK
                     KKKTSELAELISENVKVRYVDEQGRLLSLKNDTGIGEKESDGTYITNKKQLIGTSYNV
                     TDKKLSSMTTTDGKYYTFKEADTNSASLTGNIVSEGRTVTLVYRESEAPTTATVTANY
                     YKEGSQEKLAESVIKADLAIGSEYTTESKTIEGKTTTEDKEDRVITRKTTYTLVATPA
                     NAYQKTVQQLTITTVRMLRKQWFPKQQPLLRRRL"
     gene            complement(3567..3821)
                     /locus_tag="VVE42_001770"
     CDS             complement(3567..3821)
                     /locus_tag="VVE42_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073926.1"
                     /translation="MLNVDQDFMSISKSNKSGSDWKKTFTVRTTNRLANDLNNVLKQA
                     DKDTPNTPTWLNSAASKAKDDDRAYKLLKTLIPGENYLSC"
     gene            complement(4161..4694)
                     /locus_tag="VVE42_001771"
     CDS             complement(4161..4694)
                     /locus_tag="VVE42_001771"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEU3073927.1"
                     /translation="MTSFKEQEPEKVAEFLDILDNLKDLPIVYIDETGIDRYLYRPYA
                     GAPRGEKVYEKISGRRFERTSIVAGQVDGEFIAPMIYKKSMTSDFFVEWFKTQLLPAL
                     KTPHVIVMGNASFHPKNILDELCIQDKHFFLPLPPYSPDLNPIEQAWAILKKKVTDLL
                     REVPTIFECLERFFKTR"
     gene            complement(4681..4896)
                     /locus_tag="VVE42_001772"
     CDS             complement(4681..4896)
                     /locus_tag="VVE42_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU3073928.1"
                     /translation="MGKERCEQGHIERKKRVVKNRKIPLEELKAFVEAHPDAFLREIA
                     AHFDCAVPSVWAALKQIKVTLKKDDEL"
     gene            complement(4863..5006)
                     /locus_tag="VVE42_001773"
     CDS             complement(4863..5006)
                     /locus_tag="VVE42_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tnpB"
                     /protein_id="HEU3073929.1"
                     /translation="MAYSTDFKQRALDYIKEGHSHVEAAKFFGVGVRTLFTWEKKDVN
                     KDT"
BASE COUNT         1476 a         1073 c          907 g         1765 t
ORIGIN      
        1 aaaaatttaa ctaattaata gaaagtacta aatagactta tccttcaagg agttttcagc
       61 attatccatc cactcacttg ctgaaaatgt aaacagtctc tggtttatat agtaaaatga
      121 aacaagaata gagcaaatcg atcaggacag tcaaatcgat ttctaacaat gttttagaag
      181 tagatgtgta ctattctagt ttcaatctac tatacaacct caaaactgtg ttttgaacaa
      241 cctgcggcta gcttcttagt ttgccctttg attttcattg agtataaaat tgcagataaa
      301 acgataacaa tcataggact cagcagagtc aatgtaggtc gaagaatagc ccccctaatc
      361 gcttatttta tagcaaaatg aaatagaaac tgaacatttt gttgaggaag gtaaatcaat
      421 tcctcacaat gtttgagaaa gaagggagga ctattccaac ttcaacccac tataaagcag
      481 acttacaaga tagggaaccc atctccgata atatatagta gaatgaaaac tgcacaattt
      541 gattggagaa ttcaaaccaa tttctcacaa tgttttagaa acaagggtct attatttcaa
      601 ctgcaaccta ctatagtcca ttgaagctgg aatagcccac tgagacttct aaaccactgc
      661 tggagaggag tttgtttaga gtttctttca tttccctaaa aaatcaaaaa gagagaacca
      721 gtctgatcct ctcttactta tagatggtgc tcactcaaat gttaaaggag ctatttctta
      781 gagataaagg aatttgaata cagctactgc cgcaattgct gctacgatag gtgctactac
      841 tggtacccaa gaataccacc attttgaatc gcctttatgc tcaccaagaa ctgattttgg
      901 aaggaaagca tgaaggagac gtggtcccaa gtcacgggct gggttcaagg caggtcctgt
      961 aggtcctcca agtgatgtta ccaaagccat aacgaggaat ccaagtgcca agtgagccac
     1021 agaaagtcct gaagcagtgt gtggagccac ctgtgcttta atagccaagt cagaaaaatc
     1081 aactgtttgt cctgcttcta ttgccttttg tttcatgaat tgaagcactt cagcaccaaa
     1141 gaagttttta gtcaaaccaa gagctgcaaa gaacaaaaca aatgaaccaa caaactcatt
     1201 aatcaaacca ttgacagttg ctgcatagcg actttctttt gtaccatggt caatacttga
     1261 aatagttgag aaagttccca agatgttatt tgggttttca gttttcaagt agaatggacg
     1321 gtatgttgcc acaactaagg cttggccaaa gatagctccc aagacttgcg cgataatata
     1381 aggtaccact tgtgcccaag ggaaaagacc gctaactgca agccctagag tgaaagcagg
     1441 gttgatgtga ttcccagata cgttaccaaa catcaaggct gggatcataa cccccatacc
     1501 ataaccaaca gcgatgacga tccagccact ttggtgacct ttcgtacctt taagttcaac
     1561 gttggcaact gcaccattcc caagaatgat caaaatggca gttcccaaaa attcagtggc
     1621 atacttcagt gcccatgtga aatccatttg ctagattctc cttaaaattt tttagatatt
     1681 ccttatttta tcaattttta ccctatttag caactgattt ctggaaatat ttttaaaaaa
     1741 ataggattca tagtttccca gttcaacgga cttgtaagcg tttttattct tcacgaaaga
     1801 cctcgttctc aaaaccagga aaaaagccca catctgtgag cttttcctgg ttcctagatt
     1861 ctaaaatcta ttatttttca tcttcttttt tcttgaagaa tcctaatcct agactagcta
     1921 acaaggtcat gataccagca ctagccaagg tagcattagc ttctgtacct gtgtttggca
     1981 attcctctct cttacctgtc tcataagtcg gaacttctgg gtctggattc actggagttt
     2041 cagtttttgg agtacctggg tctggagttg gtttatctgg tgttgataaa cggtcatacc
     2101 ttaccgttat ttctttatca ctagagtctg acgtaacttc ttgtgattca actgttggaa
     2161 tatctggatc tttgtacttg tcaatcttac cagatacaac ctcgtcccag tttcctgttg
     2221 tccattcacc gtaggttaca actcccgtga ccttgttctc agtttttgta cggcttaagg
     2281 ttacaggttg aacaacatct tcttttacat tttggttcgt aactttatca acgtaatgaa
     2341 tgatacgcgt tatagtcttc gtctcagtag aggttgctgt tttgggaacc actgtttcct
     2401 caacattctc acggtagtaa tagtcaactg ttgcaccgtc ttctggtacg catttgcagg
     2461 agttgctacc aaggtgtatg ttgttttcct tgtgataact cggtcttctt tgtcctcagt
     2521 tgttgttttc ccttcaatag tttttgattc tgtggtatac tcagaaccta tcgctaaatc
     2581 agcttttata acagactctg ccaacttctc ttggctacct tctttatagt aattggctgt
     2641 tactgtagca gtggttggcg cttcgctttc tctataaact aaggtcactg ttctaccttc
     2701 gcttacaata ttcccagtta aacttgcaga atttgtatct gcttctttaa aagtataata
     2761 ttttccgtca gtagtagtca tgctactgag ttttttatct gtgacattgt agctggtacc
     2821 aatcagttgt tttttattgg taatgtaggt tccgtcactt tctttttctc caattccagt
     2881 atcatttttc aatgatagca aacgcccttg ttcatcaaca tagcgaactt tcacattttc
     2941 tgagattagt tctgccaatt ctgaggtttt tttctttttc ttgatttctt cggttatttt
     3001 ccctttctct tcttcgggaa tatttagttt tggaatgatt ttttcaacaa cggttcgtga
     3061 tggttccaca gtatcttgga tgactgaaaa gtcagctaga attgggagat tatactgaac
     3121 acggtgactt tgagtgttta ctcctactct ttcattattc tctgaaaata cttgtacggt
     3181 ataagaaaca acatcttttc ctaatagaac atccccagta gagaaatagc cgccttttcc
     3241 tagtttgcta tctccagagc ccacttcttt cctaatctta tcagatagtt ttttaccagt
     3301 cagtacattc gttcgcacaa tccctttgtc tacccctaca aagtgggaga actttttgaa
     3361 ctcttcagaa ccagatctag cccaaccatt attaagggca tttgcttttg tatttgtatt
     3421 ctctctcaaa agtttggcga ttagaattat attcatcggc acttagagtt gctgctatat
     3481 ctgactcttg aataccaact tccttactac catttctagc ggcagtatat gtgtatttat
     3541 ttgtttctac ttctagctga ttatccttaa catgataggt aattttctcc tggtataaga
     3601 gtcttcagta gtttatatgc tctgtcatca tctttagctt tagaagcagc tgagtttagc
     3661 caagttgggg tattaggagt atctttatca gcctgtttca agacattatt caagtcattt
     3721 gctagcctat tggttgtcct cactgtgaaa gttttcttcc agtctgaacc acttttatta
     3781 gacttagata tgctcatgaa atcctggtca acattcaaca caatatcgcc ttgtttttta
     3841 gcatcttccg ttgcttctgc ttcaaattga tcatggagtt tttgttgctc tggtgtatag
     3901 tttttttatt tacacgattt aatgtactat tttttatttt agtattaaga actccattaa
     3961 tacgattttt aaactctgct tcaaaaaatc ccttaatttg ttggtgcgca agttcaacat
     4021 tcaaaccttt cttatacaag tcttcattct gaacgatttc ggctacacgg tgaatggttt
     4081 gatcgtatac cgtcttctct taaccagcag tatccttagt tttaatgact actcgataag
     4141 ttcctttaga accgttatag tcatctagtt ttaaaaaagc gttccaaaca ttcaaaaata
     4201 gttggaactt cccttaataa atccgtcact ttctttttca agatagccca agcttgctca
     4261 ataggattca aatccggtga ataaggtggt agaggtaaga aaaagtgttt atcttggatg
     4321 cagagttcat ccaaaatgtt cttgggatga aaactagcat tgcccatgac aataacatga
     4381 ggtgttttca aagcaggtag gagttgcgtt ttgaaccact ccacaaagaa atcgcttgtc
     4441 atgcttttct tgtaaatcat gggagctata aactctccgt ctacttgtcc tgcaacaatt
     4501 gaagttcgct caaaacgacg tccgctaatc ttttcataga ctttctcccc tctaggagcc
     4561 cctgcataag gacgatagag gtagcggtcg attcctgttt cgtcaatata tacgattggt
     4621 aaatctttta ggttatccaa aatatcaaga aactcagcga ctttctctgg ctcttgttcc
     4681 ttaaagctcg tcatcttttt ttaaagtgac cttaatctgc tttaaagctg cccatactga
     4741 aggaacagca caatcaaaat gtgccgcaat ttcccgtaaa aaagcatctg gatgagcctc
     4801 tacaaaggct ttcaattcct ctaaaggaat ctttcggttt ttgacgactc gctttttcct
     4861 ctctatgtgt ccttgttcac atctttcttt tcccacgtga agagagttct gacgccaaca
     4921 ccaaaaaact tggctgcctc gacatggctg tgcccctctt tgatgtaatc taatgctcgc
     4981 tgtttaaaat cagtactata tgccatagct ttattataat atgcttgttt aaaactaagc
     5041 aactatatca atgttttgtt attagaagac ttactttttt tactgaaatc gaatttttcc
     5101 taacctcaac atcatataac cccaaaattc aatctgacct ggtttgaaga gatgttcgaa
     5161 gcagataagg ttttactaaa actaaaaaaa atgatataat caaactgatt aaatgtttta
     5221 a
//
LOCUS       DAVGJL010000119         5199 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.120, whole genome shotgun sequence.
ACCESSION   DAVGJL010000119 DAVGJL010000000
VERSION     DAVGJL010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.120"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(344..1204)
                     /locus_tag="VVE42_001774"
     CDS             complement(344..1204)
                     /locus_tag="VVE42_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEU3073930.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKPGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVTKEA
                     MYKGIEQAVVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
     gene            complement(1220..2497)
                     /locus_tag="VVE42_001775"
     CDS             complement(1220..2497)
                     /locus_tag="VVE42_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU3073931.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIERLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATMINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFLVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            complement(2490..3041)
                     /locus_tag="VVE42_001776"
     CDS             complement(2490..3041)
                     /locus_tag="VVE42_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU3073932.1"
                     /translation="MNVWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKSPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLSIITHLENEASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFNE"
     gene            complement(3057..4316)
                     /locus_tag="VVE42_001777"
     CDS             complement(3057..4316)
                     /locus_tag="VVE42_001777"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEU3073933.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASVKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
BASE COUNT         1592 a         1193 c          841 g         1573 t
ORIGIN      
        1 taatataaaa aaataaattt aagattggca atacgaacgt taaacagaat tccatttctc
       61 tttttagaat caaaacgaca atattgtgga atctctacag gaacaataga ctgcgacact
      121 aaaatatcct cccttagttt tactttacta atagtatata ggaggagagc cctgaatcct
      181 agaccctttg ttatgatgcg cttacaaaca gagttggaaa aaatcttaaa agcagaaaaa
      241 cgcatgatat cagcccctaa cacttgatac aatgcgtttt attatgaaaa gatctactgg
      301 attttttatc aaaataaact tccagtagtc ttttcacttt ttattaataa gttccttctt
      361 caccttggct agtcaagata acaggtccat ctttcgtaat gacaaattgg tgttcatact
      421 gacatgacaa tccaccgtca atggtcttat gcgcccaacc agttttcata tctgtatcaa
      481 tttcccaatc gcctgtattg atcattggtt caatggttaa gaccattcct tcacgaagac
      541 ggagtccacg acctgcaata ccatagttag gaaccattgg ttcttcgtgc atagttgggc
      601 caacaccatg accaaccaaa tcacgcacta caccgtaacc acgactttca gcgtattctt
      661 gaatagccgc accgatatca ccgatacgat ttccaacaac agcttgctca atacccttgt
      721 acatagcttc tttggttaca tccatcaagt ttttgacttc ttcggacggt gtaccaacag
      781 cataagccca acatgagtct gctaaaccac cagaatagct ctgagtgtat tttttcattt
      841 gttcaacatt gttgaagttt aattttgaga catttaggtc agatttagca atgggacctc
      901 ccaaaaccat atcaactttg agcaaatcac catctttcaa gatataatga cgagggaaag
      961 cgtgagccac ttcatcgtta agagagcaac aggtagcata aggatagtcc atcatggcac
     1021 cgtcaacccc aatctgaagt ggaaggaaat tttcttcttt acaacgacgg cggacatatt
     1081 cttcaacttc ccacatatct acgcctggct taatcaaatc acgtaagcct atatgaatac
     1141 ttgctagaaa atcaccagcc ttgtccatag cttcgatttc acgagctgat tttaatgtta
     1201 tcatcttttc tcctagtttc taattaattt taacagtcac atttgctttt gaaacaatct
     1261 ggtgaccatg ataaatatcg taatcaatta tagctgaccg tctcgtatga tgaataatcc
     1321 gtgcctgaat gcgcaatata tcatctatct gaacagcctg caaaaagtag attagcatct
     1381 gctcgataat gagattgcga ccactattaa caactaaatc ttgggtcatg tgggtcagaa
     1441 tttctgccaa tacaccatta gccaaaactc catttttttc tagcataaag ggttccactg
     1501 taatgactac ttcatcatgg tgataagaga gcttttgtcc aatctgctca gaaaaagttg
     1561 gtagagccga aacttgagaa cggctcatct tctccatgac atctcgtcgc gtcacaacgc
     1621 caagcaaagt ttgattgctt cgaacaactg gtaccatttc aaagtcttct gcgatcatcc
     1681 gttgactcac attggcaata tttgtcgata atccaaccaa aaatagacta cgagacataa
     1741 ccttatcaat tgtcgtgctt ggtgatttat caccagcgtc tctcatggtt acaacgccaa
     1801 caacgacctg atgttgattg ataacaggga aacggctgct acgattctta cgaaccaagt
     1861 ccaaataatc tttaactgta tctgtctctc tcagaaaacc atactcatga ctagggcgat
     1921 aaagtttctc aactgtcaga atatcagtct tgatttggac atttgacaag gctttattga
     1981 tcatggtcgc gacggtaaag gtatcatgct tacttcttag aacaggaatc cctttttgat
     2041 tggccagttt aagcacatca tcatgaacct gaaatccccc tgtaaccaga actgcatttt
     2101 cattttccaa ggctagcaac tgaatacggg ttcggtctcc gacaatcaag agccccccat
     2161 catgaaggta agacaagata ttttgttcag tcatggcacc aattgaaaac ttactaaatt
     2221 ctctctctaa accttcttgc ccagccagaa cctcagaaga agtcacttct gcaatttcag
     2281 caaacgttaa tctctctata gcaactttct gggatttaac acgaattgtt ccacttctag
     2341 gacgggtctc cacaattcca cggttttcag cttctttaat agcccgatag gctgttccat
     2401 cactaactcc tagatgattc gaaatgctac gaacactgac ccttttacct actggtaatt
     2461 cctccaaata gcttagaatt tcctgatgct tactcattga actctccttt tacataccga
     2521 aattcaagat aatcccgcat ttttcttgtg taacgatcac ttcccttaaa ctgacggaac
     2581 aaactaaatc cagacttaag agcaactctt tggctagctt cattttcaag gtgggtaatg
     2641 atagataatt gttttaagcc aaattcctca aaagaaagct gacaaatttt tctaacaacc
     2701 tctgtcataa atccttgcga ccaagcatct tttctcaaaa aatagccaag ctcagcttct
     2761 tttttgattt catctaactt ctcaaattta atagaaccaa tcatttgttg atttttctgg
     2821 tcacaaattg cccacactcc caaaggggac ttcataaagt aattggccag tgcatattga
     2881 ctttcttcca gacttgcctg cgttgggaaa ataaattgaa gattttctgg atttgaagct
     2941 atctcgcgga agtcctgact atcactaaaa aagaaaggac gcaaatacaa gcgatccgtt
     3001 tcaaaaaaag aaaacattgc taatttggtc caaacattca taaacacctc tacggtttaa
     3061 tcctcaacaa gtctaatatc cgctcctaaa ttacgtaatt tttcgataat atcagaataa
     3121 ccacgtaaga taaactcgat attggtaatt tcagttttac cttcagccat aagcccagca
     3181 atgactagtg cagccccagc tcttaagtca gtcgctttaa cactggcccc acgtaaatca
     3241 cgtccacccg tgtacaaaat atgaccattt gttgtcgaaa tatccgcatc catctttgct
     3301 agttcaaaaa catgatttac acgtttttcg taaatcgtat cgacaattgt accacgacca
     3361 ttcgctctta gtaaaagagg ggtaagcggt tgttgcaaat cagttgcaaa gcctgggtaa
     3421 ggagctgtct taatattgat tgctttcaaa ttagactgtt cctcgacaaa aatgctgtct
     3481 tcagatacag tcattctcac tcccatttct tccaacttag caataaaccc ttccaggtgt
     3541 tcgtaaagaa cattatttat acgaattcct ttaccaactg cagcagctaa agatatatat
     3601 gttccagctt caatgcggtc tggaatcacc tgatgacgtg tcccatgtaa tctttcaaca
     3661 ccatcaataa taatgatatt agttcctgcc ccacggatat gggcacccat attattcaag
     3721 agagtagcta catcaataat ctcaggttca cgggctgcat tttcaataat agtacgacca
     3781 tttgctttaa ctgcagcaat catcgtatta atcgttgctc ccacactaac cgtatccatg
     3841 taaatacttg caccatgaag tcctgtatct ttagcagata acttcatgtt atctccctcg
     3901 tagctagcag tggcacccat agcttcaaac gccttaaggt gtaagtcaat cggacgagga
     3961 ccaagatcac atcctcccgg tagaccaact gtcgcttcac caaaacggcc taagaggctc
     4021 ccataaaaat agtaagatgc acgaagactg ttaattttac cataaggcat tggaatattt
     4081 tgaacacctc ttggatcaat ctccaagaca tcgtcataac gcttaacagt agctcccatc
     4141 aattccatga tttcgacaag actggctaca tccgaaatat ctggaacaca atccaaagtc
     4201 accacatcat cagccaatat aatagctgga attaaggcca caacactatt tttagcacca
     4261 ctaatagtaa tttcaccttg cagtggtaat ccaccattga taacaatttt tctcattcta
     4321 agttttcaat actctttcaa gatttctaat aaggtcttca aaataaagta tatcataaat
     4381 ttatcctttt ttccatcctt ttcaatttta ttggataaat cagagttgac gagttagtaa
     4441 ctgaattact aatactcaat aaaaatcaaa gatcaaacta ggaaactagc cgcaggttgc
     4501 tcaaaacact gttttgaggt tgtagatgga actgacgtag tcagtatcct acacgtacgg
     4561 caaggcgata ttgacgtgat ttgaagagat tttcgaagag tataatcttt ctcgaatcgc
     4621 tattgccctt ttttatcctc tgaaaccgag taggagctaa gactagatct cttatctaag
     4681 tatcggaata cggctgttca actaaatttt atcaagaact tctccccctt ctctcagttc
     4741 acagaagcgt tttatacata ccgtccctat atttcatgaa caagacgttt tcaggctagc
     4801 atcctatcag acaatctatt ttaaagataa ccgtgactca ggatcgcttc tatctgtcgt
     4861 tttctcttta tagtgataga cctcacggga taagtcgtac atctttcctc gtgtatacaa
     4921 gatataagga cgttaaaagg ttacagtgaa aatagaaaat cttagtacga attcaattac
     4981 tctcgtgatt tacgcatata ggttacgact acctttttgg actttagagt ttgaggccct
     5041 cttatccgct atatacgcct tattatcacg ttcctgttcg tagagccacg atttcgctat
     5101 cccttcttct gacaattctt atttccagcg tcagacttaa aacggtctat ccccagacca
     5161 ttttaatccg ctacctcacg atagtcaggc ttgggaatc
//
LOCUS       DAVGJL010000120         5187 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.121, whole genome shotgun sequence.
ACCESSION   DAVGJL010000120 DAVGJL010000000
VERSION     DAVGJL010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5187
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.121"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            320..1865
                     /locus_tag="VVE42_001778"
     rRNA            320..1865
                     /locus_tag="VVE42_001778"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1917..1989
                     /locus_tag="VVE42_001779"
     tRNA            1917..1989
                     /locus_tag="VVE42_001779"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1950..1952,aa:Ala,seq:tgc)
     gene            2114..5015
                     /locus_tag="VVE42_001780"
     rRNA            2114..5015
                     /locus_tag="VVE42_001780"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            5092..>5187
                     /gene="rrf"
                     /locus_tag="VVE42_001781"
     rRNA            5092..>5187
                     /gene="rrf"
                     /locus_tag="VVE42_001781"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1468 a         1049 c         1517 g         1153 t
ORIGIN      
        1 gttggtcaag gggttaagac accgcctttt cacggcggta acacgggttc gaatcccgta
       61 cggactatgg tatgttgcgg ttggaacact tgatgaaaaa aagtttaaaa aaacttaaaa
      121 atcttcaaaa aaagtgttga caagtgaaag tggctgtgat atactaatat agttttcgct
      181 tgagagaagc aagtgacaaa gacctttgaa aactgaacaa gacgaaccaa tgtgcagggc
      241 gctacaacat aagttgtagt actgaacaat gaaaaaacaa taaatctgtc agtgacagaa
      301 atgagtgaga actcaaactt ttaatgagag tttgatcctg gctcaggacg aacgctggcg
      361 gcgtgcctaa tacatgcaag tagaacgctg aaggaggagc ttgcttctct ggatgagttg
      421 cgaacgggtg agtaacgcgt aggtaacctg cctggtagcg ggggataact attggaaacg
      481 atagctaata ccgcataaga gtggatgttg catgacattt gcttaaaagg tgcacttgca
      541 tcactaccag atggacctgc gttgtattag ctagttggtg gggtaacggc tcaccaaggc
      601 gacgatacat agccgacctg agagggtgat cggccacact gggactgaga cacggcccag
      661 actcctacgg gaggcagcag tagggaatct tcggcaatgg acggaagtct gaccgagcaa
      721 cgccgcgtga gtgaagaagg ttttcggatc gtaaagctct gttgtaagag aagaacgagt
      781 gtgagagtgg aaagttcaca ctgtgacggt atcttaccag aaagggacgg ctaactacgt
      841 gccagcagcc gcggtaatac gtaggtcccg agcgttgtcc ggatttattg ggcgtaaagc
      901 gagcgcaggc ggttagataa gtctgaagtt aaaggctgtg gcttaaccat agtaggcttt
      961 ggaaactgtt taacttgagt gcaagagggg agagtggaat tccatgtgta gcggtgaaat
     1021 gcgtagatat atggaggaac accggtggcg aaagcggctc tctggcttgt aactgacgct
     1081 gaggctcgaa agcgtgggga gcaaacagga ttagataccc tggtagtcca cgctgtaaac
     1141 gatgagtgct aggtgttaga ccctttccgg ggtttagtgc cgtagctaac gcattaagca
     1201 ctccgcctgg ggagtacgac cgcaaggttg aaactcaaag gaattgacgg gggcccgcac
     1261 aagcggtgga gcatgtggtt taattcgaag caacgcgaag aaccttacca ggtcttgaca
     1321 tccctctgac cgctctagag atagagtttt ccttcgggac agaggtgaca ggtggtgcat
     1381 ggttgtcgtc agctcgtgtc gtgagatgtt gggttaagtc ccgcaacgag cgcaacccct
     1441 attgttagtt gccatcattt agttgggcac tctagcgaga ctgccggtaa taaaccggag
     1501 gaaggtgggg atgacgtcaa atcatcatgc cccttatgac ctgggctaca cacgtgctac
     1561 aatggctggt acaacgagtc gcaagccggt gacggcaagc taatctctta aagccagtct
     1621 cagttcggat tgtaggctgc aactcgccta catgaagtcg gaatcgctag taatcgcgga
     1681 tcagcacgcc gcggtgaata cgttcccggg ccttgtacac accgcccgtc acaccacgag
     1741 agtttgtaac acccgaagtc ggtgaggtaa ccgtaaggag ccagccgcct aaggtgggat
     1801 agatgattgg ggtgaagtcg taacaaggta gccgtatcgg aaggtgcggc tggatcacct
     1861 cctttctaag gataaggaac tgcgcattgg tcttgtttag tcttgagagg tcttgtgggg
     1921 ccttagctca gctgggagag cgcctgcttt gcacgcagga ggtcagcggt tcgatcccgc
     1981 taggctccat tggtgagaga tcaccaagta atgcacattg aaaattgaat atctatatca
     2041 aatagtaaca agaaaataaa ccgaaaacgc tgtagtatta ataagagttt atgactgaaa
     2101 ggtcagaaaa taaggttaag ttaataaggg cgcacggtgg atgccttggc actaggagcc
     2161 gacgaaggac gtgacaaacg acgatatgcc ttgggtagct gtaagtaagc gatgatccag
     2221 ggatttccga atgggggaac ccaacaggta atacctgtta cccacatctg ttaaggatgt
     2281 gaggaggaag acgcagtgaa ctgaaacatc taagtagctg caggaagaga aagcaaaagc
     2341 gattgcctta gtagcggcga gcgaaacggc agaagggcaa accgaagagt ttactcttcg
     2401 gggttgtagg actgcaatgt ggactcaaag attatagaag aatgatttgg gaagatcagc
     2461 caaagagagt aatagcctcg tatttaaaat agtctttgta cctagcagta tcctgagtac
     2521 ggcgggacac gtgaaatccc gtcggaatct gggaggacca tctcccaacc ctaaatactc
     2581 cctagtgacc gatagtgaac cagtaccgtg agggaaaggt gaaaagcacc ccgggagggg
     2641 agtgaaatag aacctgaaac cgtgtgccta caacaagttc gagcccgtta atgggtgaga
     2701 gcgtgccttt tgtagaatga accggcgagt tacgttatga tgcgaggtta agttgaagag
     2761 acggagccgt agggaaaccg agtctgaata gggcgcctta gtatcatgac gtagacccga
     2821 aaccatgtga cctacccatg agcaggttga aggtgcggta agacgcactg gaggaccgaa
     2881 ccagggcacg ttgaaaagtg cttggatgac ttgtgggtag cggagaaatt ccaaacgaac
     2941 ttggagatag ctggttctct ccgaaatagc tttagggcta gcgtcgacat tagagattct
     3001 tggaggtaga gcactgtttg ggtgaggggt ccatcccgga ttaccaatct cagataaact
     3061 ccgaatgcca atgaattatg gtcggcagtc agactgcgag tgctaagatc cgtagtcgaa
     3121 agggaaacag cccagaccac cagctaaggt cccaaaataa ttgttaagtg gaaaaggatg
     3181 tggggttgca cagacaacta ggatgttagc ttagaagcag ctattcattc aaagagtgcg
     3241 taatagctca ctagtcgagt gaccctgcgc cgaaaatgta ccggggctaa aacaatttac
     3301 cgaagctgtg gataccttta taggtatggt aggagagcgt tctatgtgtg atgaaggtat
     3361 accgtgagga gtgctggaac gcatagaagt gagaatgccg gtatgagtag cgaaagacag
     3421 gtgagaatcc tgtccaccgt aagactaagg tttccagggg aaggctcgtc cgccctgggt
     3481 tagtcgggac ctaaggagag accgaaaggt gtatccgatg gacaacaggt tgatattcct
     3541 gtactagagt atgtagtgat ggagggacgc agtaggctaa ctaaagcaga cgattggaag
     3601 agtctgtcta agcagtgagg tgtgaattga gtcaaatgct taattctata acattgagct
     3661 gtgatgggga gcgaagttta gtagcgaagt tagtgacgtc acactgccaa gaaaagcttc
     3721 tagcgtttaa acatactcta cccgtaccgc aaaccgacac aggtagtcga ggcgagtagc
     3781 ctcaggtgag cgagagaact ctcgttaagg aactcggcaa aatgaccccg taacttcggg
     3841 agaaggggtg ctgacttaaa gtcagccgca gtgaataggc ccaagcaact gtttatcaaa
     3901 aacacagctc tctgctaaat cgtaagatga tgtatagggg gtgacgcctg cccggtgctg
     3961 gaaggttaag aggagtgctt agcgtaagcg aaggtatgaa ttgaagcccc agtaaacggc
     4021 ggccgtaact ataacggtcc taaggtagcg aaattccttg tcgggtaagt tccgacccgc
     4081 acgaaaggcg taatgatttg ggcactgtct caacgagaga ctcggtgaaa ttttagtacc
     4141 tgtgaagatg caggttaccc gcgacaggac ggaaagaccc catggagctt tactgcagtt
     4201 tgatattgag tgtctgtacc acatgtacag gataggtagg agtctaagag atcgggacgc
     4261 cagtttcgaa ggagacgctg ttgggatact acccttgtgt tatggccact ctaacccaga
     4321 taggtgatcc ctatcggaga cagtgtctga cgggcagttt gactggggcg gtcgcctcct
     4381 aaaaggtaac ggaggcgccc aaaggttccc tcagaatggt tggaaatcat tcgcagagtg
     4441 taaaggtata agggagcttg actgcgagag ctacaactcg agcagggacg aaagtcgggc
     4501 ttagtgatcc ggtggttccg tatggaaggg ccatcgctca acggataaaa gctaccctgg
     4561 ggataacagg cttatctccc ccaagagttc acatcgacgg ggaggtttgg cacctcgatg
     4621 tcggctcgtc gcatcctggg gctgtagtcg gtcccaaggg ttgggctgtt cgcccattaa
     4681 agcggcacgc gagctgggtt cagaacgtcg tgagacagtt cggtccctat ccgtcgcggg
     4741 cgtaggaaat ttgagaggat ctgctcctag tacgagagga ccagagtgga cttaccgctg
     4801 gtgtaccagt tgtcttgcca aaggcatcgc tgggtagcta tgtagggaag ggataaacgc
     4861 tgaaagcatc taagtgtgaa acccacctca agatgagatt tcccatgatt atatatcagt
     4921 aagagccctg agagatgatc aggtagatag gttagaagtg gaagtgtggt gacacatgta
     4981 gcggactaat actaatagct cgaggactta tccaaagtaa ctgagaatat gaaagcgaac
     5041 ggttttctta aattgaatag atattcaatt ttgagtaggt attactcaga gttaagtgac
     5101 gatagcctag gagatacacc tgtacccatg ccgaacacag aagttaagcc ctagaacgcc
     5161 ggaagtagtt gggggttgcc ccctgtg
//
LOCUS       DAVGJL010000121         5124 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.122, whole genome shotgun sequence.
ACCESSION   DAVGJL010000121 DAVGJL010000000
VERSION     DAVGJL010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5124
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.122"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            47..724
                     /locus_tag="VVE42_001782"
     CDS             47..724
                     /locus_tag="VVE42_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HEU3073934.1"
                     /translation="MIDLDNPPYSIIDLVIWGVFVIDYSWRFFTTKRKWRFILENIFD
                     LLAILPLNAIFTVFRLGRIFRLAKLTKLLKLTRLLRIIGLTGKLERKISRFLRTNGLI
                     YILYVNIFIVLVGSSILSVVEEKSFSDSLWWALVTVTTVGYGDIVPASIFGKWLAVLL
                     MLVGIGTIGMLTSALTNFFVKDNPDEQIKLDKLQDELSSQRILLEKQSKKIEELHKMI
                     QDLIEKT"
     gene            complement(987..1973)
                     /locus_tag="VVE42_001783"
     CDS             complement(987..1973)
                     /locus_tag="VVE42_001783"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEU3073935.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGQYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            2167..4635
                     /gene="gyrA"
                     /locus_tag="VVE42_001784"
     CDS             2167..4635
                     /gene="gyrA"
                     /locus_tag="VVE42_001784"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001152996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEU3073936.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIVLRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMVGQILSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLAEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLIITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEA"
     gene            4635..>5124
                     /gene="srtA"
                     /locus_tag="VVE42_001785"
     CDS             4635..>5124
                     /gene="srtA"
                     /locus_tag="VVE42_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HEU3073937.1"
                     /translation="MSQKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIVW
                     NTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWNAQKLPVIGGIAIPE
                     LEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDNA
                     KNG"
BASE COUNT         1566 a          864 c         1139 g         1555 t
ORIGIN      
        1 acttgcttta atctctgtta ttctggtatt acttggattt gctgagatga ttgatttgga
       61 caatccgcct tatagtatta ttgatttagt aatctggggt gtttttgtaa ttgattatag
      121 ttggcgcttt tttacgacta aaagaaaatg gcgctttatt cttgaaaata tttttgattt
      181 attggctatt ctccctttaa atgctatttt tacagtgttt cgattagggc gtatctttcg
      241 tttagctaaa ctaacaaaat tactgaaact gactcgttta cttagaataa ttggacttac
      301 aggtaaatta gaaagaaaaa ttagtagatt cttacggaca aatggcttga tttatatctt
      361 gtatgttaat atctttatcg tgctagtagg aagttcaatt ttatctgtag ttgaagaaaa
      421 atccttctca gatagtcttt ggtgggctct tgtaacggta acgactgttg gatatgggga
      481 tattgttcct gcttccattt ttgggaaatg gctagcagtt ttactgatgc ttgtcggtat
      541 tggaacaata gggatgttaa caagtgcttt gacgaacttt tttgtaaagg ataatccaga
      601 tgaacagata aaacttgata aactccaaga tgaattatct agtcagagaa tattactaga
      661 gaaacaatct aaaaagatag aagaactaca taaaatgata caagatttga ttgaaaaaac
      721 ataataaaaa taaagatgag aaaaagtttg aactgaggag aaatcaagta agttttgaca
      781 caatagaacg catattatca ggtataaaaa cttgatacta tgcgttttat tgtgggaaga
      841 tgtataatgg attgaaataa gatatgaaca aatcgattag gaatttaaag cattttataa
      901 caacgtttta gagtaacggg gtactatttc atataataca ggaaaaaaca actccctgat
      961 gattcaagga gttgtctata gttaaattag tttttagaag cttcttggaa ttctggattt
     1021 ttccatgctt cgtcaataat agcttgcaat tctttagcag atgcttgcat tttttgagtt
     1081 tctgcatcgt tcaatggaat gtttactgga cgaacgatac catgtgcacc aacaacagct
     1141 ggttgaccga taaagacatt ctcaactccg tattgacctt cttggaatac tgaaagtgga
     1201 agtactgcgt tttcatcgtc aaggattgct ttagtgatac gagcaagggc tactgcgata
     1261 ccgtagtatg ttgcaccttt tttgttgatg attgtgtagg ctgcatcacg aacaccttcg
     1321 aacaattcaa tcaattcagc ttcttgaaca ttttgagtgt ctttaaggaa ttcttcaagg
     1381 tttacaccag cgatgttagc gtgtgaccaa acagcgaact cagagtcacc gtgttcaccc
     1441 atgatgtagg cgtgcactga acgagcatcc acatccaatt tttcagcaag tgcttgacgg
     1501 aaacgagctg agtcaagtga agtacctgaa ccgataacgc gttctttagg gaaaccagag
     1561 aatttccaag ttgagtaagt caaaacgtca actgggttag cagcaacaag gaagatacct
     1621 ttgaaaccag attcaacaac ttgagttacg attgatttgt tgatagcaag gtttttacct
     1681 acaaggtcaa gacgagtttc acctggtttt tgaggtgcac ctgcagtgat cacaacaagg
     1741 tcagcgtctg cacagtcaga gtattgagct gcatagattt ttttaggtga agtgaaggca
     1801 agggcgtgac taaggtcaag cgcatcacca acagcttttt catgcaattg tggaatttcg
     1861 ataattccaa gctcttgtgc aattccttgg ttaacaagtg caaaagcgta agatgaacct
     1921 acagcaccat caccgacaag gataactttt ttgtgttgtt tagttgaagt cattgtttta
     1981 aacatctcct taattttatt aggggatttt ccctagacaa cttcattcta tcacttttaa
     2041 aaaactttgt cacgaatatg ccttatagtt ctcgatgtaa acgttttagt ggtttagagg
     2101 ctgaaataga tgggaattta tggtataatg ttgttactta ctaattgtga aatgaggcat
     2161 ttattaatgc aggataaaaa tttagtgaat gtcaatctga caaaggagat gaaggcaagt
     2221 tttatcgact acgccatgag tgttatcgta gcgcgagctc ttcctgatgt tcgagatggc
     2281 ttaaaacctg ttcaccgtcg cattctctac ggaatgaatg aattgggtgt gaccccagac
     2341 aaaccccata aaaaatctgc tcgtattaca ggggatgtca tgggtaaata tcacccacac
     2401 ggggattcct ctatttatga agccatggtc cgtatggctc aatggtggag ctaccgttat
     2461 atgcttgtag atggtcatgg gaattttggt tccatggatg gagatagtgc tgccgctcaa
     2521 cgttataccg aggcacgtat gagcaagatt gctctggaaa tgcttcgtga tatcaacaaa
     2581 aatacagttg atttcgttga taactatgat gccaatgaac gggaaccctt ggtcttgcca
     2641 gcgcgttttc caaacctttt ggttaatgga gcaactggta tcgcggttgg gatggcaacc
     2701 aatattccac ctcataatct gggtgaaacc attgatgcag tgaagttggt catggataat
     2761 cctgaagtga ctaccaagga cttgatggaa gtcttgcctg gaccagattt tccaactggt
     2821 gctcttgtca tggggaaatc aggtatccat aaggcctatg aaacaggtaa aggttcgatt
     2881 gtcctacgtt ctcgtacaga gattgaaacg actaagactg gtcgtgagcg catcgttgta
     2941 acagaatttc cttacatggt caataaaacc aaggtgcatg agcatattgt tcgcttggtt
     3001 caggaaaaac gcattgaggg tatcacagca gtacgtgatg agtcaaaccg tgaaggtgtt
     3061 cgatttgtta ttgaagtcaa gcgcgacgcc tcagccaatg ttattctcaa taacctcttc
     3121 aaaatgaccc aaatgcaaac caattttggt ttcaatatgc tcgctatcca aaatggtata
     3181 ccgaaaattt tgtctcttcg tcagattttg gatgcttata tcgagcacca aaaagaagtg
     3241 gttgttcgtc gtacacgttt tgataaggaa aaagcggaag cgcgcgctca tatcttagaa
     3301 ggtctcttga ttgcgctaga ccatatcgac gaagtgattc gtatcatccg tgctagtgaa
     3361 acggatgcgg aagctcaagc tgagttgatg agcaagttta agctttctga acgtcaaagt
     3421 caagctatcc ttgatatgcg tcttcgtcgt ttgacaggtt tggaacgcga taagattcaa
     3481 tctgagtatg atgacctctt ggctctgatt gcggatttag cagatattct tgctaagcct
     3541 gaacgtgttt ctcaaattat caaagacgaa ttggatgaag ttaaacgtaa attttctgat
     3601 aaacgccgta cagagttgat ggttggacag atcttgagtc tcgaggatga ggacttgatt
     3661 gaagaatcgg atgtcttgat taccctttct aacagaggct acattaagcg tttggatcag
     3721 gacgagttca ctgctcaaaa acgtgggggt cgtggtgtcc aaggaacggg agtgaaagat
     3781 gatgactttg ttcgtgagtt agtgtcaact agcacacatg atcatctgct cttcttcaca
     3841 aacaagggac gtgtctatcg tcttaaaggt tatgaaattc ctgagtatgg tcggactgcc
     3901 aaagggctac cagtagtcaa tctcttgaaa ttggatgaag acgaaagtat tcagacggtt
     3961 atcaatgttg agtctgatcg cagtgatgat gcttatctct tctttacaac ccgtcacggt
     4021 attgtgaaga gaactagtgt taaggagttt gccaatattc gtcaaaatgg tctcaaagcg
     4081 ctgaatttaa aggatgaaga tgagttaatc aatgtcttgt tggcagaagg agatatggat
     4141 attatcattg gtaccaagtt tggttatgca gttcgcttta atcaatcagc cgttcgtggt
     4201 atgagccgta tcgccactgg tgtgaaaggt gttaaccttc gtgaaggaga cacagttgtt
     4261 ggtgccagct tgattactga tcaagatgag gttcttatta tcacagaaaa aggatatggt
     4321 aagcgtacag tcgctactga atacccaaca aaaggtcgtg gtggtaaggg aatgcagaca
     4381 gctaaaatta ccgaaaaaaa tggcttgctg gccggtctta tgactgttca aggggatgag
     4441 gatttgatga ttatcactga tacaggtgtc atgattcgaa ccaatcttgc caatatttca
     4501 caaacaggac gtgcaactat gggagttaaa gtaatgcgcc tggatcaaga tgctcagata
     4561 gtgactttca caacggttgc ggtggcagaa aaagaagaag ttgggacaga aaacgaaaca
     4621 gaaggtgaag cataatgtct caaaaaaata ataaaaagaa aaacaagcga aaaaatctgc
     4681 tgacaaatat cctagcagga tttctgatat tactgtcact ggctttgatt tttaatactc
     4741 aaattcgaaa tattttcata gtctggaata ccaataagta tcaagttagc caggtatcaa
     4801 aagaaaaatt agaagaaaat caggatacag aaggcaattt tgactttgat tctgtcaaag
     4861 ctatctcttc ggaagctgtt ctaacttctc aatggaatgc tcaaaaatta ccagttattg
     4921 ggggaattgc aattcctgaa ttggaaatga atttgccgat ttttaaagga cttgataatg
     4981 ttaatctctt ctacggagct ggtacaatga aacgcgagca agtaatggga gaaggaaatt
     5041 atagtctagc tagtcaccat atctttggtg ttgataatgc taataaaatg ttattttctc
     5101 ctttagataa tgctaaaaat ggca
//
LOCUS       DAVGJL010000122         4953 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.123, whole genome shotgun sequence.
ACCESSION   DAVGJL010000122 DAVGJL010000000
VERSION     DAVGJL010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4953
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.123"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(46..1935)
                     /locus_tag="VVE42_001786"
                     /pseudo
     CDS             complement(46..1935)
                     /locus_tag="VVE42_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
     gene            2074..3639
                     /locus_tag="VVE42_001787"
     CDS             2074..3639
                     /locus_tag="VVE42_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEU3073938.1"
                     /translation="MRKIYLSIFTSLLLMLGLVNVAQADEYLRIGMEAAYAPFNWTQD
                     DDSNGAIKIDGTNQYANGYDVQIAKKIAKDLGKEPLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTAERKQEIAFSSSYYTSEPVLLVKKDSAYASAKSLDDFNGAKITSQQGVYLYD
                     LIAQIPGAKKETAMGDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMIQVEPGF
                     KTGEEDTAIAIGLRKNDNRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSS
                     SFFSQVAKILSENWQQLLRGAGITLLISIVGTIIGLIIGLAIGVFRTAPLSENKAIYG
                     LQKLVGWVLNVYIEIFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYM
                     TEIVRGGILAVDKGQFEAATALGMTHNQTMRKIVLPQVVRNILPATGNEFVINIKDTS
                     VLNVISVVELYFSGNTVATQTYQYFQTFTIIAVIYFVLTFTVTRILRFIERRMDMDTY
                     TTGANQMQTEDLK"
     gene            3639..4403
                     /locus_tag="VVE42_001788"
     CDS             3639..4403
                     /locus_tag="VVE42_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU3073939.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IVAQTTVLKRERTEAEEIAKENLEKVGMGERYWQAKPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLILNENQRAN"
     gene            complement(4602..4817)
                     /locus_tag="VVE42_001789"
     CDS             complement(4602..4817)
                     /locus_tag="VVE42_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073940.1"
                     /translation="MAKSNFEKVESVVGWVRDKKITGYRISKETNAREMSIIALAQGR
                     AKVKNISFETALGLIDFYEKNYEKFED"
BASE COUNT         1538 a         1110 c          895 g         1410 t
ORIGIN      
        1 ctatcttcca ttatatcata ctttttcagt ctatggtagc tctctttaaa aggcaccgat
       61 actgccgcca cctccgcctc cagagaagcc accaccagaa cttacacttc cagaagatac
      121 agagtaggtg cttgctgtat ttgcgacact agcataatgg ctcatttgtg ctgttgaatg
      181 ataaaacata ctgtgccagc cataagctac atagaaattg atatctggat tttcaacttg
      241 aatctgatga accttcatca aatgactaac cttgtccgca tagtcaaata aggttgcata
      301 gaccaagaga cgattccaaa caacaatact ttccaattca gccttatcca gtcgtgcaat
      361 ctcacgtaac atattttcaa aactggtcca gagatagtag acctcagctc ccgcttcatt
      421 tagaacacca tcacgattat ctagtcgaag cttccaatga tagaaaacag acaaaactaa
      481 ccctagaaaa ccaagtattg gcaaagggag gtaaagatag ccatgaacgt ctaaactgta
      541 caagaacaaa ccaaatccga taaatagggg caagatagtc aagacaccca tacccacttg
      601 caaagtcttt tccaaaccag ttaaaggacg atagtaatct gggagccccc agaaggaaac
      661 tcgttttctc actccttctt gcatctggtt caatacctct tcaaaagaag atttgagttg
      721 aagccctctt gcttgaatcc gtttttcatc agaaactttg gctctacgat aaagactatc
      781 agataccttg taatccgcaa acaaattgga aagagtttct tcttttttac ctgaaaaagc
      841 tagatttagg cagtctttct caaagcttga caaaccatct tcttttacta gcctcaaacc
      901 aactgcatct ccttctgaaa tgatagagac attcccacgg tctatcacat ctagcaaggt
      961 agcttgaata agttgatcaa aggtgaattt tccagctccc ttgaccaagg gactcacttc
     1021 ctccaaggag gtcgagtaga ctgcttctga taaaaccata ggctctaatt ccattggtgg
     1081 ttcatagaga cgatgatttt tggcatattt gactgaagga gtggtctttc ttctataaat
     1141 aaaatagaag cagacactca ataacaagga gatagaaagt atcgaaggga ggacccaagt
     1201 aacgagttgt ttactctgat ctttttctct aacaatcgag tcttctatct tattaaactc
     1261 ttctaatcga ttccctttca atccctgatc cctagcgcta gcaaaatcgg tccgaggtca
     1321 ataggcatgc aactcaactc cacgcctagc cggaagattg tctaaacgga tagtataatc
     1381 aaggttactc ttttcaatcg ttccctctct aaaaagtttc cctgtatgga aaaagagttt
     1441 ttcagccccc ttgtctcccc ttacatgaaa ttcaaacttt tcaatagact ctgaactatc
     1501 tgtcagaggt tgccaattta attcagcgat atcatcataa aggaaaagta aattttttaa
     1561 gttccagacg aggtcaactt caactatgtc gccctcctga cctggattat agactctcac
     1621 agtataacca tccgcttctt ctgttacttc gctagtcaca tctgctagtt ctgcaccgtt
     1681 tttcgcggcc tgaacctttg gatgagggtc aatgtcaaac ccgctaggca tcttaccagc
     1741 acgtccaagt cccacgattt gtcccttaaa gtcctcctca aactgataaa ctatcttttg
     1801 tctaaattct gccgtattgt ctgcatgaat atacaaatca ccttgataag agtttatctt
     1861 aaaatcaatg gcgaaaacag aaagtggcag aaggcaaaac aagccaaaca tcagtaagaa
     1921 aaaagttttt ttcatcaatt aagccccctt tttatctttc ttatcattat agcatttttt
     1981 cgtaagtaaa ggcttactta tgttgaaaaa ttacgcattt ttcgataaaa taggagacag
     2041 ttatttttta atagattaga aataggagaa atcatgagaa aaatatactt atctattttc
     2101 acaagtctct tgctgatgct aggacttgtc aatgttgctc aagccgatga atatttacgc
     2161 atcgggatgg aagcggcata tgctcccttt aactggaccc aagatgatga tagcaatgga
     2221 gctatcaaaa tcgatggaac caaccagtac gccaatggat atgatgtcca aattgccaag
     2281 aaaatcgcta aggacttagg taaagaacct ttggttgtta aaaccaagtg ggaaggtcta
     2341 gtccctgccc ttacttctgg taagattgac atgattatcg caggtatgag tccaactgca
     2401 gaacgcaaac aagaaattgc cttttcgagc agttactaca ctagcgaacc agttttgctt
     2461 gtcaaaaaag attctgccta cgcaagtgct aaatctttgg atgactttaa cggtgcaaaa
     2521 atcacttctc aacaaggggt ctacctctac gacttgattg cacaaatccc aggtgctaaa
     2581 aaagaaacag ccatgggaga cttcgctcaa atgcgacaag ctcttgaggc tggtgtcatt
     2641 gatgcttatg tttctgaacg tccagaagca ctgactgctg aagctgcgaa ctctaagttc
     2701 aagatgattc aagtagaacc tggtttcaaa actggggaag aagatacagc tatcgctatc
     2761 gggcttcgta aaaatgacaa tcgtattagc caaatcaatg ccagcattga aaccatttca
     2821 aaggacgacc aagttgccct gatggatcgt atgatcaagg aacaacctgc cgaagctaca
     2881 acaactgaag agactagcag tagtttcttt agccaagtcg ctaaaattct ttctgaaaac
     2941 tggcaacaac tcttgcgtgg tgctggtatc actcttttaa tctctatcgt cggaaccatc
     3001 ataggtctca ttattggact tgccattggt gtcttccgta ctgctcctct ctctgaaaac
     3061 aaagccattt atggccttca aaaactagtc ggctgggttc tcaatgtcta cattgaaatt
     3121 ttccgtggta cgccaatgat tgttcaatcg atggttatct actatggaac tgcccaagct
     3181 ttcgggatca accttgaccg tacactggct gctatcttca tcgtttcaat caataccggt
     3241 gcctacatga ctgaaatcgt ccgtggtggt atcctagcag ttgacaaggg acaatttgaa
     3301 gctgcgactg ctcttggtat gacccataac cagaccatgc gtaagattgt cctacctcag
     3361 gtagtccgta acatcctacc tgcaactggt aatgaatttg tcatcaatat caaagataca
     3421 tctgtattga acgttatctc tgttgtcgaa ctttatttct caggaaatac cgtggcaaca
     3481 caaacctatc aatacttcca gacatttaca atcatcgccg tgatttactt tgtcctcacc
     3541 ttcaccgtaa cacgtatcct acgctttatc gagcgcagaa tggacatgga tacctacact
     3601 acaggtgcta accaaatgca aacggaggat ttgaaataat gacacaagca atccttgaaa
     3661 ttaaacacct caaaaaatcc tatggacaaa acgaagtgct aaaagacatt tctctcactg
     3721 tccacaaggg agaagttatt tctatcatcg gaagctctgg aagtgggaaa tcaaccttcc
     3781 tacgctccat taacctactt gaaacgccaa ctgatggaca aatcctttat catggacaaa
     3841 acgtcctcga aaaaggctat gacctcacgc aataccgtga aaagttaggg atggttttcc
     3901 aatcctttaa cctctttgaa aatctcaacg ttcttgaaaa cacaatcgtc gctcagacaa
     3961 ctgtccttaa acgcgaacgc acagaagctg aagaaattgc caaagaaaac ctggaaaagg
     4021 tcggcatggg agaacgctac tggcaagcga aacccaaaca actctcaggt ggtcaaaaac
     4081 aacgtgtggc cattgctcgt gccctttcaa tgaatcctga cgccattctc tttgatgaac
     4141 caacatcagc tctcgatcca gaaatggttg gagaggtcct caaaatcatg caagatctgg
     4201 ctcaggaagg cttgaccatg attgtcgtaa cccatgaaat ggaatttgcc cgtgatgtct
     4261 ctcaccgtgt tatctttatg gataagggtg tgattgctga agaaggtaaa ccagaagacc
     4321 tcttcactaa tcctaaagaa gaccgtacaa aagaatttct tcaacgttat ctcatactca
     4381 atgaaaatca aagagcaaac taggcagcta gccgcaggtt gctcaaaaca ctgttttgag
     4441 gttgcagata gagctgacgt ggtttgaaga gattttcgaa gagtatcaaa taaaaagaaa
     4501 aggctgcatc aaccgtgcag tctttttgct gtcctcaaaa tgaaaaggca gattctctat
     4561 ctctactact tgaaggtcaa gaatccgccg ttatccaaag attaatcttc aaatttttca
     4621 taattttttt catagaaatc aattaggcct agggctgttt caaatgaaat attttttact
     4681 tttgcacgac cctgcgccag agcaatgata gacatttcac gcgcattcgt ttctttagag
     4741 atacggtagc ctgtgatttt cttatcacga acccagccaa caactgattc tactttttca
     4801 aagtttgact tagccatgtt tttctcctat tttcttcttt ataatattta attgtatacg
     4861 tttttactac ttttgtcaat aaaaaaaaca tatattcaac gaaaataatt agttcagaat
     4921 tttcttactt cctttttaaa tccgataata tat
//
LOCUS       DAVGJL010000123         4809 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.124, whole genome shotgun sequence.
ACCESSION   DAVGJL010000123 DAVGJL010000000
VERSION     DAVGJL010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4809
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.124"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            29..478
                     /locus_tag="VVE42_001790"
     CDS             29..478
                     /locus_tag="VVE42_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEU3073941.1"
                     /translation="MTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHSSSSSSSSI
                     ESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTLSPKKKSAE
                     ATVATKSPVTITLGVVKGVDLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            489..1034
                     /gene="pgsA"
                     /locus_tag="VVE42_001791"
     CDS             489..1034
                     /gene="pgsA"
                     /locus_tag="VVE42_001791"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEU3073942.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIMLIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            1031..1858
                     /locus_tag="VVE42_001792"
     CDS             1031..1858
                     /locus_tag="VVE42_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEU3073943.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKRGEWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRQIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALALVGMLDFKKREPARLSGGQKQRVAIAGVVALRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPRELFSRNDLDQIGLDDPFANQLKKSLSQNGYDLPENYLTESELEDKLWELL"
     gene            1843..2682
                     /locus_tag="VVE42_001793"
     CDS             1843..2682
                     /locus_tag="VVE42_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEU3073944.1"
                     /translation="MGIALENVNFTYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQLLNGLLVPSQGSVRVFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPAILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            2675..3469
                     /locus_tag="VVE42_001794"
     CDS             2675..3469
                     /locus_tag="VVE42_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEU3073945.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKAMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            3528..4346
                     /gene="mreC"
                     /locus_tag="VVE42_001795"
     CDS             3528..4346
                     /gene="mreC"
                     /locus_tag="VVE42_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEU3073946.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTIVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLL
                     DMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKISQLNSNSDISAGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            4346..>4809
                     /gene="mreD"
                     /locus_tag="VVE42_001796"
     CDS             4346..>4809
                     /gene="mreD"
                     /locus_tag="VVE42_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEU3073947.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLVYDVYFFHLIGITTLLFILLGAFLHKLNSVILLNRW
                     TRMLAMIVLTFLFEMGSYLLAFMVGLTVDSMSIFIVYSLVPTMILNFLWITV"
BASE COUNT         1406 a          724 c         1070 g         1609 t
ORIGIN      
        1 cctttttgct ttatcgattt taatttttgt gacttattat gtttggaact atattcaaac
       61 tcaaccagag gagccttctc tttctaatta cagtgtggtt caatcaacaa gttcaactag
      121 ctctgttccc cactcctcaa gtagtagttc ttctagtata gaatcagcta taagtgtatc
      181 aggcgaagga aatcatgtag aaatcgctta taagacaagt aaggaaacag ttaaattgca
      241 attggcagtt tcagatgtta caagttgggt cagtgtttca gaaagcgaac ttgagggcgg
      301 tgtaacctta tcgccaaaga agaaaagtgc agaagcaaca gttgcaacta aaagtcctgt
      361 aacaattacg ttaggtgttg taaaaggtgt tgatttgaca gtagataatc agactgttga
      421 tttatcgaaa ttaacagctc agactggaca aatcactgta acctttacta aaaattaagg
      481 aaaaacgaat gaaaaaagaa caaattccca atctcttaac aataggtcga attctcttta
      541 tacctatttt tatctttatt ttaacgatag gaaattcgat agagagtcat atagttgcag
      601 ctattatctt tgctgttgcc agtattaccg actatttaga tggatattta gctcgtaaat
      661 ggaatgtggt cagtaatttt ggtaaatttg cagatcctat ggcggataag ttactagtta
      721 tgtcggcttt tattatgttg attgagttag gtatggctcc ggcttggatt gttgcagtga
      781 ttatctgtcg tgagttagct gtgacaggtt taaggctttt attggttgaa actggtggaa
      841 caattttagc agcagcaatg cctggaaaaa ttaaaacttt tagtcagatg tttgccatta
      901 ttttcttgct attacattgg actttgcttg gtcaagttct actttatgta gccttatttt
      961 tcactatcta ctctggctat gactatttca agggtagtgc ctatgtattt aaagggacat
     1021 ttggttcgaa atgaaatcaa taattgatgt aaaaaatctt tcttttcgct ataaagaaaa
     1081 tcagaactac tacgatgtga aggatattac gtttcacgtg aaacgtggag aatggctttc
     1141 gattgtaggg cataatggta gtggtaaatc aacgacggtt cgattaatag atggcttact
     1201 ggaagcagaa tccggagaga ttgtaattga tggccaacgt ttgactgagg aaaatgtttg
     1261 gaatatacgt cgtcaaatcg gtatggtttt tcaaaatcca gacaatcaat ttgttggagc
     1321 gactgttgaa gatgatgttg cttttggttt ggaaaatcag ggactttctc gtcaagaaat
     1381 gaaaaagaga gtggaagaag ctctggcttt agttggcatg ttggacttta aaaagagaga
     1441 gccagcgcgt ctatcaggtg gccaaaagca acgtgtggcc attgcaggtg ttgtagccct
     1501 aagaccagct attttaatct tagatgaagc aacgagtatg ttggatcctg aggggcgtag
     1561 agaacttatt gggacagtaa aaggaattcg aaaagactat gatatgacgg tcatttctat
     1621 tacccatgat ttggaagaag tcgccatgag tgatcgcgta ttggtcatga aaaaagggga
     1681 aattgaatca actagtagtc caagggagct tttctctcga aatgatttag atcaaattgg
     1741 attagacgat ccttttgcta atcaattaaa aaaatctttg agccagaatg gctatgattt
     1801 acctgaaaat tatttgacag aaagtgagct agaggataag ctatgggaat tgctctagaa
     1861 aatgtgaatt ttacatatca agaaggtact cccttagctt cagcagcttt gtcggatgtt
     1921 tctttgacga ttgaagatgg ctcttataca gctttaattg ggcacacagg tagtggtaaa
     1981 tcaactattt tacaactctt aaatggttta ttggtgccaa gtcaagggag tgtgagggtt
     2041 tttgatacct taatcacctc gacttctaaa aataaagata ttcgtcaaat tagaaaacag
     2101 gttggcttgg tatttcagtt tgctgaaaat cagatttttg aagaaacggt tttgaaggac
     2161 gttgcttttg gaccgcaaaa ttttggagtt tctgaagaag atgctgtgaa gactgcgcgt
     2221 gagaaactgg ctctggttgg aattgatgaa tcgctttttg atcgtagtcc gtttgagctg
     2281 tcagggggac aaatgagacg tgttgccatt gcaggcatac ttgccatgga gccagctata
     2341 ttagtcttag atgagccaac agctggtcta gatcctctag ggagaaaaga gttgatgacc
     2401 ctgttcaaaa aactccacca gtcagggatg accatcgtct tggtaacgca tttgatggat
     2461 gatgttgctg aatatgcaaa tcaagtctat gtaatggaaa agggacgttt agtaaagggg
     2521 ggcaaaccaa gtgatgtctt tcaagacgtt gtttttatgg aagaagttca gttgggagta
     2581 cctaaaatta cggccttttg taaacgattg gctgatagag gcgtgtcatt taaacgatta
     2641 ccgattaaga tagaggagtt caaggagtcg ctaaatggat agtatgattt tggggcgtta
     2701 tatcccaggg gattcgattg ttcaccgatt ggatccacgt agcaaattgc ttgctatgat
     2761 gctactgatt ttgattgtgt tttgggctaa taaccccttg acgaatctga ttctttttat
     2821 agcgacaggg atatttattg ccttgtcagg agtatctctt tcatttttta ttcagggctt
     2881 gaagtctatg tttttcttga ttgccttcac aactattttt caactatttt tcatttctaa
     2941 tgggaatgtt ttatttgagt tttcgtttgt gagaatcacg gattatgctt tgcaacaagc
     3001 tgggattatt ttttgtcgct ttgtattgat tattttcttt tcaactttgt taaccttaac
     3061 aaccatgcct ttaagtttgg catcagctgt cgaagcttta ttagcacctt taaagcgtgt
     3121 gaaagttcca gttcatgaaa ttggattgat gctgtctatg agtttgcgtt ttgtcccaac
     3181 cttgatggat gatacgactc ggattatgaa tgcacagaaa gctcgtggag tggattttgg
     3241 agaaggaagc atcgttcaaa aagtaaaggc gatgattccc attttgattc ctctttttgc
     3301 gacaagttta aaacgtgcag attccttggc tatcgctatg gaagcgcgtg gctatcaggg
     3361 tggaaaaggc agaagtcaat accgacaatt gaaatggact ctaaaggata cgctgaccat
     3421 tcttgttatt ctcgtacttg gttgttgttt atttttctta aaatcttagt agctttcagg
     3481 aattgataaa aagttactgt aacagttttt gatataaagg tagggatatg aaccgtttta
     3541 aaaaatcaaa atatgtcatt attgtttttg tcactgttct gcttgtgtca gctctcttag
     3601 cgacgactta ttcaagtaca attgtgacaa aattaggaga tggaatctca ttggttgata
     3661 gagttgtaca aaaacctttt cagtggtttg attctgtcaa atcagatttg gctcatttga
     3721 cacgaacata taatgaaaat gaaagtttga agaaacagct ttaccaatta gaagttaaat
     3781 caaatgaggt ggaaagttta aagacagaaa atgaacaact gcgccaattg cttgatatga
     3841 agtctaaatt gcaagccaca aagactttag cagcagatgt tattatgcgt tctccggtat
     3901 cttggaagca ggagttgacc ttagatgcag gtagatcaaa aggtgcttct gagaacatgt
     3961 tagctattgc aaatggtggc ttgattggga gtgtttcaaa agtagaggag aactctacta
     4021 tagtcaacct tctgacaaat acggaaaatg ctgataagat ttctgttaaa attcaacatg
     4081 gctctactac aatttatgga attattattg gctatgacaa ggaaaatgac gttcttaaaa
     4141 ttagccaatt aaatagtaat agcgatatta gtgcgggaga taaggtgact actggtggat
     4201 taggaaactt taacgttgct gatattcctg ttggtgaagt ggttgccaca acgcatagta
     4261 cagactattt gacacgagaa gtaactgtta aattgagtgc agatactcat aatgtagatg
     4321 tgatagaatt agtggggaat tcataatgag acagttgaag cgagttggag tatttttatt
     4381 gcttcctttc tttgttctaa ttgacgccca tattagccag cttctgggct catttttccc
     4441 ccatgtacat ttggctagtc attttctttt tctatttctc ttatttgaga cgatagaagt
     4501 atcagagtat ctctacctag tctattgttt tgttataggc ttggtttatg atgtttactt
     4561 tttccatcta atagggatta caactctctt atttatctta ttgggagcct tccttcataa
     4621 attgaatagt gttattttgt tgaatcgttg gacaagaatg ctagctatga ttgtgctgac
     4681 attcctgttt gaaatgggta gttatctttt ggcttttatg gtagggttga cagtagatag
     4741 catgtcgatt tttatagtct atagcttggt accgacgatg attttaaatt ttttatggat
     4801 tactgtttt
//
LOCUS       DAVGJL010000124         4787 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.125, whole genome shotgun sequence.
ACCESSION   DAVGJL010000124 DAVGJL010000000
VERSION     DAVGJL010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4787
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.125"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(28..399)
                     /locus_tag="VVE42_001797"
     CDS             complement(28..399)
                     /locus_tag="VVE42_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0218 family bacteriocin-like peptide"
                     /protein_id="HEU3073948.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGAIYIPRWAITGAITGAAYAAL
                     AAAGGGGLQLVLASYGLRSALVAGIVKGLGVLGIHIGNAFANTVIRSIASAGIGAGAD
                     WIFTNIIDGWDGRRDNQLRIG"
     gene            complement(544..1896)
                     /locus_tag="VVE42_001798"
     CDS             complement(544..1896)
                     /locus_tag="VVE42_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEU3073949.1"
                     /translation="MKLYNKSELRYSRIFFDKRPPAFAFILIISTAIILSGALVGAAY
                     IPKNYIVKANGNSVITGTEFLSAIGSGKVVTLHKSEGDMVNAGDVIISLSSGQEGLQA
                     SSLNKQLVKLRAKEAIFQKFEQSLNEKYNRMSNSGEEQEYYGKVEYYLSQLNSENYNN
                     GTQYSKIQDEYTKLNKITAERNQLDADLQTLQNELIQLQQQGDSPSLSDTTSADDKAK
                     LETKILEITTKIEALKTNITSKNSEIDSQQSNIKDMNRTYNDPTSQAYNIYAQLISEL
                     GTARSNNNKSITELEANLGVATGQDKAHSILASNEGTLHYLVPLKQGMSIQQGQTIAE
                     VSGKEKGYYVEAFVLASDISRVSKGAKVDVAITGVNSQKYGTLKGQVRQIDSGTISQE
                     TKEGNISLYKVMIELETLTLKHGSETVILQKDMPVEVRIVYDKETYLDWILEMLSFKQ
                     "
     gene            complement(1893..2360)
                     /locus_tag="VVE42_001799"
     CDS             complement(1893..2360)
                     /locus_tag="VVE42_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU3073950.1"
                     /translation="MAKLEVKDNKKLVLKSVICKKLHDTKVEDVDQEINKFHQHLQLL
                     KAQIFGPLIVKSCGTTIHDDGLITTDFEFYIQAHNAQQYSNIYDVQDSISVPYCLYVR
                     FEDSPEYLQYAYSKLDLYVYENDIQTDGIVYTVYVNSSPEKMVVDIFRPIVSL"
     gene            complement(2353..4572)
                     /locus_tag="VVE42_001800"
     CDS             complement(2353..4572)
                     /locus_tag="VVE42_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001856353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HEU3073951.1"
                     /translation="MLKKYPCTMQHDQSDCAAAVVSTVLLSYKKELSIMKIREIIGTD
                     MYGTTVSGIVSGLNKLNFTVKAVRVALEDLTPKLTFPAILQVKNDLGQNHFVVLHSIK
                     RNSKFYVAAPASGIRKMSSDELGEIYQGITLFMVPNSDFERGKLKGKGLLDLFGRLIF
                     NQKGLISTVILASFVLSIIGILSSLFSKVIMDEVIPYALKNSLYMFLIVFGIVSFLQT
                     LLSAFRQHVLLFLSRKIDIPVLMGYYDHIIHLPYSFFGSRRVGDVLTRFQDAMTIKNV
                     FTSVSISLVMDITLSVISAVVLWTINQSLFLILVFMVIVNIILIYCFKKPYKKINHEQ
                     MEANGLLNSQLIESIRNIDTIKSQHDEEQRLNKIEEKFVHTLEIGYKEGVLQNIQSTI
                     SSMTSTMGGLLFMGVGALFIIDGKMTIGDLLVFQTLSQYFTEPIQNLVGLQLTFQEVQ
                     VAVSRLQELMEVDREDIALDYSIRDFTLCDDIEFKDVTFAYGSRPPVIKDFNLRIKQG
                     EKIAFVGESGAGKSTLVRLLLRFINPSEGKIRIGENDLSDLDYGKLRKKISYIPQTIE
                     LFTGTIIDNLKIGNPSVTYEDMVRVCRIVGIHDTIQRLQNRYGSFVEEGGQNFSGGEK
                     QRLAIARALLSKADLYIFDEATSNLDSFSEQIIQDLIFNKIMDKTTIVVAHRLSTILR
                     CDKICFLENGTIVEYGTHEELMAKNGKYARMVGLQSVQVNQQIQSQAVLDTEEVTYG"
BASE COUNT         1529 a          975 c          664 g         1619 t
ORIGIN      
        1 gaaattaaaa caatcaacat aagattatta acctattctc aattgattat cacgtcgccc
       61 atcccagcca tcaataatat tggtaaaaat ccaatcagct ccagcaccaa taccagcaga
      121 tgcaatactt ctaataacag tatttgcaaa agcatttcca atatgaattc ctaatactcc
      181 taaaccttta acaatcccag ctaccagtgc ggagcgtaat ccataagatg caagaactag
      241 ttgaaggcct ccacctcctg ctgctgctaa tgctgcatat gctgcgccag taatggctcc
      301 tgtaattgcc cacctgggta tatatatagc ccccccatca aggtacatca tctcttcttc
      361 atcaatcaca acataattat ttggtaatac aagttccatt tgttttctcc tttttattaa
      421 gcatggttag gatggtttcc taacaagtaa tcgtcgactg tgtttctatc acttagctac
      481 ctagataaaa tatcctttct ctaccttatt tgtttttata ggttacctaa ggtttaaaac
      541 caattattgc ttgaaactta acatttctaa aatccaatca agataggttt ctttatcata
      601 gacaatccgc acttcaactg gcatatcctt ttggagtatg accgtctcgc ttccatgttt
      661 tagagttaag gtttctaatt ctatcatgac tttatagagg ctaatattcc cctctttcgt
      721 ttcttgggaa attgttcctg aatcaatctg tctgacttgt ccctttagtg ttccatattt
      781 ttgactattt acaccagtaa tagcaacatc aacttttgct ccttttgaaa cacgagaaat
      841 atcactcgca agtacaaaag cctctacata gtaacctttt tctttccctg aaacttctgc
      901 tatcgtttgc ccctgctgaa tagacattcc ttgtttcaaa ggtaccagat aatgcagagt
      961 accttcattt gacgctaata tactatgagc tttatcttga cctgttgcca ctccaagatt
     1021 agcctcaagc tctgtaatac ttttattgtt gtttgaacga gcagtaccta actcactaat
     1081 taattgagca taaatattat aagcctgaga agttggatca ttataggtac ggttcatatc
     1141 cttaatattg ctttgttgac tatcaatctc gctattctta gaagtaatat ttgtttttaa
     1201 tgcttcgatt tttgttgtta tttctaatat cttagtttct aacttagctt tatcatcagc
     1261 tgatgtcgta tctgataagg aaggggagtc tccttgctgt tgaagttgaa tcaattcatt
     1321 ttgtagagtt tgcaagtcgg catctaactg atttctttca gctgttattt tattcaactt
     1381 cgtatattca tcctgaatct ttgaatactg ggtaccatta ttataatttt ccgaatttaa
     1441 ctgagatagg tagtattcaa ctttcccata atattcctgt tcttcaccag aattagacat
     1501 acggttgtat ttctcattta atgattgttc aaatttttga aagatagctt cttttgcacg
     1561 taacttcacc aattgtttat ttaaagagct cgcttgtaaa ccttcttgtc cacttgataa
     1621 cgaaataatg acatctccag catttaccat atctccttct gacttatgta aagtaactac
     1681 cttccctgaa ccaattgctg ataggaactc tgtacctgtt ataactgaat ttccattcgc
     1741 ttttacaata tagtttttgg gtatataagc tgcaccaacc aatgcaccgc ttaagataat
     1801 agcagttgaa ataatgagaa taaacgcaaa agctggtggt ctcttatcaa agaaaatacg
     1861 agaataacgt aattctgatt tattatataa tttcataggc ttacaattgg tctaaaaata
     1921 tctactacca ttttttcagg agaagaatta acataaactg tatagacaat cccatccgtt
     1981 tgaatatcat tttcatagac atatagatcc aatttagaat acgcatactg tagatactct
     2041 ggactgtctt caaaacgaac atataaacaa tatggaacag agatagaatc ctgtacatca
     2101 taaatgttac tgtactgttg agcattatga gcttgaatat aaaactcaaa atcagtcgtt
     2161 attaatccat catcatgaat agtagtacca caacttttta caattaatgg accaaaaatt
     2221 tgtgctttta acaactgcaa atgttgatga aatttattaa tttcctgatc tacatcttct
     2281 acttttgtat catgtaactt tttacagata actgacttta gtaccagttt tttattatct
     2341 tttacctcta acttagccat aagtaacctc ctctgtatct aacacagcct gtgactgaat
     2401 ttgttgattc acttgaacgc tctgcaaacc aaccattcta gcatactttc catttttcgc
     2461 cattagttct tcatgagtcc catattccac aatagtccca ttttctaaaa agcatatctt
     2521 atcacatcga agaattgtag acagcctgtg ggctactaca attgttgtct tatccattat
     2581 tttattaaag attaaatcct gaataatctg ttcactaaat gaatctaagt tagaggttgc
     2641 ctcatcaaat atatacaaat cagctttact cagtagtgct cttgcaatag ccaatcgttg
     2701 tttctctcca cccgagaaat tttgaccgcc ttcttctaca aaggagccat aacgattttg
     2761 aagacgttga atcgtatcat gaataccaac aatacgacaa actctcacca tatcctcata
     2821 tgtaacagaa ggattaccaa tttttagatt atcaataatt gtccctgtaa agagttctat
     2881 ggtctgtgga atgtaggaaa ttttcttacg tagtttacca tagtcaagat ctgataagtc
     2941 attttctcct atacgaattt tcccctcact cggatttata aatcttaata acaaacgaac
     3001 tagcgtactt tttcctgctc cgctttctcc aacaaaagct attttttctc cttgtttaat
     3061 tcgcaaatta aagtccttga taacaggagg gcgtgaacca taggcaaatg ttacatcttt
     3121 aaattcaatg tcatcacata aggtgaagtc tctaatacta taatccaagg caatgtcttc
     3181 acgatctact tccatcaatt cttgaagccg actaaccgca acctgtacct cttgaaaggt
     3241 taactgcaag ccaactaaat tctgaatagg ttctgtaaaa tattgactta atgtctgaaa
     3301 aaccaagagg tccccaatag tcatttttcc atcaatgata aataaggcgc ccactcccat
     3361 aaataataac ccacccatgg ttgaggtcat tgaagatatt gtagattgaa tattctgcaa
     3421 aacaccctct ttataaccaa tttctaaagt atgaacaaat ttttcttcaa ttttgtttaa
     3481 acgttgttcc tcatcatgct gagacttaat ggtatcaatg ttacgaatag attcaattaa
     3541 ctgtgaatta agaagtccat ttgcttccat ttgttcatga ttaatttttt tataaggctt
     3601 tttaaaacag tatataagaa tgatgttaac aataaccata aaaactaata tcagaaataa
     3661 agattgatta atagtccaaa ggacaaccgc actaataact gacaaggtga tatccatgac
     3721 taatgaaata gagactgacg taaacacatt ctttatcgtc atcgcatctt gaaatcttgt
     3781 taaaacatca ccaactctac gacttccaaa aaaactataa ggaagatgaa taatatggtc
     3841 ataatatccc attaaaaccg gtatatcaat ttttcgagat agaaacaaaa gaacgtgctg
     3901 acgaaatgca gatagaagag tctgtaagaa cgacactatt ccaaatacta tcaaaaacat
     3961 atacagacta ttttttaaag catatggaat cacctcgtcc atgataactt ttgaaaagag
     4021 actcgataaa ataccaatga tacttaaaac aaaggaagct aatatgactg tactaatcaa
     4081 tcctttttga ttaaaaatta accttccaaa taaatcaaga agtccttttc cctttagttt
     4141 ccctctttca aaatccgaat ttggaaccat aaaaagagta ataccttgat aaatttctcc
     4201 cagttcgtca cttgacatct tgcgtatgcc actagcaggg gctgctacgt agaacttact
     4261 attccttttg atactatgta ataccacaaa atgattttga cctaaatcat tcttaacttg
     4321 aagaatcgca ggaaatgtta attttggagt caaatcttcc agtgctactc gaacagcttt
     4381 tactgtaaaa ttcaacttat tcagacctga aacaatacca ctgacagtcg ttccatacat
     4441 gtctgtacca ataatttccc taatcttcat gattgatagt tcctttttgt aagataaaag
     4501 aactgttgaa acaactgccg cagcacaatc tgactggtca tgttgcatcg tacaaggata
     4561 tttttttaac atagaaatac tcaattcttt agaattaatc taacttttct ttgatgtcac
     4621 ccaaacctca atcactgccc agtagttctt gaattaattt attttcaacc gtatcattct
     4681 ttgttcttga tatctttcca agcattgtag tacagcagat ttttgcccaa ggactgagcc
     4741 tcctttaaca actcaaactc ccttacatta tatcggccag ccccatg
//
LOCUS       DAVGJL010000125         4787 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.126, whole genome shotgun sequence.
ACCESSION   DAVGJL010000125 DAVGJL010000000
VERSION     DAVGJL010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4787
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.126"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            18..236
                     /gene="nrdH"
                     /locus_tag="VVE42_001801"
     CDS             18..236
                     /gene="nrdH"
                     /locus_tag="VVE42_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEU3073952.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPGKLKQLA"
     gene            317..2476
                     /gene="nrdE"
                     /locus_tag="VVE42_001802"
     CDS             317..2476
                     /gene="nrdE"
                     /locus_tag="VVE42_001802"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEU3073953.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWAELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            2664..3626
                     /gene="nrdF"
                     /locus_tag="VVE42_001803"
     CDS             2664..3626
                     /gene="nrdF"
                     /locus_tag="VVE42_001803"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEU3073954.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSAED
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEAMQDDDYNYGLD"
     gene            3808..4569
                     /locus_tag="VVE42_001804"
     CDS             3808..4569
                     /locus_tag="VVE42_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU3073955.1"
                     /translation="MLKQEKLTKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
                     QNLSNLQFSKAFVSCNGIQNGALATFSEEEGEAQRIALNNSNKKYLLADHSKFNKFDF
                     YTFYNVSNLDTIVSDSKLSDSILFKLSKHIKVIKP"
BASE COUNT         1528 a          975 c          882 g         1402 t
ORIGIN      
        1 tgaagaggag ttacacaatg gtaaccgttt attctaaaaa caattgtgtc caatgtaaaa
       61 tgaccaagcg tttcttggac agtaataatg tctcttatcg tgaaatcaat cttgatgagc
      121 aacctgagta cgtcgatcaa gttaaagagc tcggttttag cgcagctcct gttatccaaa
      181 caccaactga agtcttttca ggtttccaac caggaaaact gaaacaatta gcataatctt
      241 agtacatcat ccagaagaaa ctgcttttag ggctaactta gaagcctttc ttttgtaatt
      301 agataaggga aattttatgg gattaaaaca tcttgaagac gtgacttact tccgtcttaa
      361 taacgaaatt aaccgtcctg ttaatggaca aatcatgctt cataaagata aggaagcctt
      421 ggatgctttc tttaaagaaa atgtagttcc aaacactatg gtttttgatt caatcaaaga
      481 caaaatcaat tacctcattg aacacaacta catcgaaaca gcctttatca agaaataccg
      541 tccagagttc ttggaagaat tggctcaatt tatcaaagac caaaacttcc aattcaagtc
      601 attcatggca gcttataaat tttacaatca atatgcctta aagactaatg acggtgaata
      661 ctatcttgaa aatatggaag accgtgtctt cttcaacgcc ctttatttcg ctgatgggaa
      721 tgaagctgtt gcaatcgata ttgccaatga aatcatccac caacgctacc aacctgctac
      781 tccttccttc ttgaatgctg gacgtgctcg tcgtggggag ttggtatctt gtttcctaat
      841 ccaagtgacg gatgatatga actctatcgg gcgttctatc aactcagctc ttcaactttc
      901 acgtattggt ggtggtgtgg gaattaccct cagcaacctt cgtgaagctg gtgcacctat
      961 caaaggctat gaaggagctg cttcaggtgt cgttcctgtt atgaagcttt ttgaagacag
     1021 cttctcttac tccaaccaat tggggcaacg tcaaggtgcc ggcgttgtct acctcaacgt
     1081 ctttcaccca gatatcatcg ctttcctttc aactaagaaa gaaaacgctg atgaaaaagt
     1141 acgtgtcaag actctatcac ttggtgttgt agtacctgat aaattctacg aattggctcg
     1201 taaaaatgaa gaaatgtacc tcttcagccc ttactctgta gaaaaagaat acggtgtgcc
     1261 attcaattac atcgacatta ctgaaaaata cgatgaattg gtcgcaaatc caaatattcg
     1321 caagacaaaa atcaaggcgc gggatttgga aactgaaatc tctaaattgc aacaagagtc
     1381 tggctatcct tatgtagtca acatcgatac ggctaaccgt gcaaatcctg ttgatggaaa
     1441 gattatcatg agtaacttgt gttctgagat tcttcaagtt caagaaccaa gcttgatcaa
     1501 cgatgctcaa gaattccttc aaatgggaac agatgtttca tgtaacctgg gttcaaccaa
     1561 cgtggttaac atgatgactt cacctgattt tggtcgttct atccgtgcta tggttcgtgc
     1621 ccttactttc gttacagata gttcacatat cgtagctgta ccaacgattg accatggaaa
     1681 tagccaagcc cacacctttg gtctcggtgc catgggactg cacagctacc ttgcccaaca
     1741 actcattgaa tatggttcac ctgagtctgt tgaatttaca agcatctact ttatgcttat
     1801 gaactactgg accttggtag agtcaaacaa tattgcgcgt gaacgtggta ttaccttcca
     1861 caactttgaa aaatcagact atgctaacgg aagttacttt gacaagtatg taactggtga
     1921 atttgttcca acatcagacc gtgttaaaga actcttcaaa aatgttttta tccctggtgt
     1981 tgctgattgg gctgaacttc gcgacaaggt tcaagaagat ggtctttacc accaaaatcg
     2041 ccttgctgta gcgccaaatg gttctatcag ctatatcaac gacgtttctg cttctatcca
     2101 cccgattacg caacgtatcg aagaacgcca agagaagaaa attggtaaaa tctactaccc
     2161 tgctgctggc ttgtctacag aaaccattcc ttactacact tctgcctacg atatggatat
     2221 gcgtaaagtc atcgatgttt acgctgctgc gactgaacac gtggatcaag gactttcact
     2281 caccctcttc atgcgcagtg acattccaaa aggcctttac gaatggaaga gggaaaataa
     2341 acaaacgaca cgtgatttgt ccatccttcg taactatgcc tttaacaagg gaatcaagtc
     2401 tatctactac gtccgtacct ttacagatga cggtggagaa gtcggcgcca accaatgtga
     2461 aagctgtgtg atttagtttc tagcatagaa atattatttt tccctaagca gcactgataa
     2521 aatcaaatct actatgtcat aacataattt atgtaacatt ttaaaagtcg ggcttccgac
     2581 tttttgtgcg atactcctat agaattatgg taaagtagaa ataattgtga gttcgcaata
     2641 ctaaacacag aaagagattt taaatggaaa cttactacaa agccattaac tggaatgcca
     2701 tcgaagatgt catcgacaaa tcaacttggg aaaaactgac ggagcaattt tggctcgata
     2761 cacgtattcc cttatcaaac gacttggatg actggagaaa gctatctaac aaagagaaag
     2821 acttggtcgg aaaagttttt ggtggtttaa cccttcttga cactatgcaa tctgaaactg
     2881 gggttcaagc ccttcgcgca gacatccgta caccacatga ggaagctgtt ttcaataaca
     2941 tccaatttat ggaatctgtc cacgctaaat cttactcatc aatcttttct accttgaata
     3001 ctaaggctga gattgaagaa attttcgaat ggaccaatac caatccttac ctacaaaaga
     3061 aggctgagat tgtcaacgaa atctacctaa acggcagccc acttgaaaag aaagttgcca
     3121 gcgtcttcct cgaaaccttc ctcttctact ctggtttctt cactcccctc tactatctcg
     3181 gtaacaacaa actagccaac gttgcagaaa tcattaaatt gattattcgt gacgagtctg
     3241 ttcacggaac ctacattggt tacaaattcc aacttggttt caatgaattg cctgaagaag
     3301 agcaagaaaa actcaaagaa tggatgtacg acctactcta tactctttat gaaaatgaag
     3361 aaggctacac agagagtctt tatgacggtg ttggttggac tgaagaggtt aaaaccttcc
     3421 ttcgctacaa tgccaataaa gctctcatga acatgggaca agatccactt ttcccagatt
     3481 cagctgaaga tgtcaaccca atcgttatga acggtatttc aacaggaact tccaaccatg
     3541 acttcttctc tcaagtcgga aatggttacc ttcttggcga agttgaagcc atgcaagacg
     3601 atgactataa ctacggttta gactaattct ccatcactct caatcatcaa aaagtaaact
     3661 agaaacatca aagttataac ccaacaaaaa tatcgaaagt tttgaaaaca aacttcgata
     3721 tttttgtttt tgtttcattt tgttgttttt gtatttgaat gatgagtttg tttatggtaa
     3781 gatagtagta ggaaacgagg tgataatatg ctcaaacaag aaaaattaac caaaatttta
     3841 gagatagtaa atagtaaagg aaccataaca gttaaacaga ttatggacga aatagccgtt
     3901 tcagatatga ctgcaaggcg ctatttacag gaattagctg ataaagattt gctgattcgt
     3961 gtgcatggtg gagctgaaaa acttcgaacc aactcccttt tgactaatga gcgatcaaat
     4021 attgaaaaac aagccctcca aacggcagaa aaacaagaaa tagcccattt tgcaggcagt
     4081 ctagtagaag aaagagaaac tattttcatt ggaccaggaa caacattaga gttttttgcg
     4141 cgtgagttgc ctattgacaa tatccgcgtc gtaaccaaca gtctacctgt ttttctgatt
     4201 ttaagcgaac gaaaattaac agatttgatt ttaataggtg gaaattatcg cgatattaca
     4261 ggtgcttttg ttggtacatt gaccctacaa aatctctcta atctccaatt ttctaaagct
     4321 ttcgttagct gtaatggtat tcaaaacgga gctctagcta cttttagcga ggaagaggga
     4381 gaggctcaac gcatcgcttt aaataattct aataaaaaat atttactcgc agatcatagc
     4441 aagttcaata agtttgattt ttatactttt tataatgtat caaatcttga tactattgtt
     4501 tcagattcta aactaagtga ttcaatcctt tttaagctat ctaaacacat taaagtcatc
     4561 aagccttaat aaactgaaag agcctgggta acaactcaat ttcagaagaa aaaaagtaaa
     4621 tcttcccaca ataaaacgca tagtatcagg tgttgaaaca cctgatacta tgcgtttttc
     4681 tatatcaaag actttttgcc caattcctca aagctatgca ttgagaaatc tgcgactcgc
     4741 ttccttgttt aaagttgctc tttgattttc attgagtata agcaacg
//
LOCUS       DAVGJL010000126         4754 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.127, whole genome shotgun sequence.
ACCESSION   DAVGJL010000126 DAVGJL010000000
VERSION     DAVGJL010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4754
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.127"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(135..206)
                     /locus_tag="VVE42_001805"
     tRNA            complement(135..206)
                     /locus_tag="VVE42_001805"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(171..173),aa:Arg,seq:cct)
     gene            complement(248..595)
                     /gene="rplS"
                     /locus_tag="VVE42_001806"
     CDS             complement(248..595)
                     /gene="rplS"
                     /locus_tag="VVE42_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEU3073956.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            complement(714..1043)
                     /gene="crcB"
                     /locus_tag="VVE42_001807"
     CDS             complement(714..1043)
                     /gene="crcB"
                     /locus_tag="VVE42_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEU3073957.1"
                     /translation="MVIVYLAIACGLGALVRYFFSRYNQASKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLTLTYIGGLVA
                     IFLGILL"
     gene            complement(1037..1411)
                     /gene="crcB"
                     /locus_tag="VVE42_001808"
     CDS             complement(1037..1411)
                     /gene="crcB"
                     /locus_tag="VVE42_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEU3073958.1"
                     /translation="MKKEQFYPLGIFLAAMLGGLVRYLVSTWLPASPDFPWGTLFVNY
                     LGIFCLIYLVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDTVKLLDTGRYLSLI
                     LYLLLSIGGGLLLAYFLGRKKW"
     gene            complement(1408..1674)
                     /locus_tag="VVE42_001809"
     CDS             complement(1408..1674)
                     /locus_tag="VVE42_001809"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEU3073959.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEGVVAYKKASGKPILD
                     TKREAVIFEKVRSRVEDKRYQETIVATFSDILKCSRDYQDQNIK"
     gene            complement(1790..2233)
                     /locus_tag="VVE42_001810"
     CDS             complement(1790..2233)
                     /locus_tag="VVE42_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEU3073960.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRVFVSTGAEKGSECVKVDLSAEEEDIERLEQFAEELVAKVG"
     gene            2337..3272
                     /locus_tag="VVE42_001811"
     CDS             2337..3272
                     /locus_tag="VVE42_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEU3073961.1"
                     /translation="MEICQQILEKIKEYDTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQEVLKQYNVTDAETAAIVGAPGRIDRVSLWGIFVEQADGHYRVRLRSKIHPINEIA
                     KEHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            3326..3610
                     /locus_tag="VVE42_001812"
     CDS             3326..3610
                     /locus_tag="VVE42_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEU3073962.1"
                     /translation="MARKETMAERKYCKMKKDIHPEYRPVVFMDTTTGYQFLSGSTKR
                     SNETVEFEGETYPLIRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(3667..3885)
                     /locus_tag="VVE42_001813"
     CDS             complement(3667..3885)
                     /locus_tag="VVE42_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073963.1"
                     /translation="MSISPRFETLEQAIASKDLEKVREAFKKMNSTWTINESVVRYNS
                     IAHYGRVETAISFLPSSMETEPTDESGT"
     gene            complement(3904..4426)
                     /locus_tag="VVE42_001814"
                     /pseudo
     CDS             complement(3904..4426)
                     /locus_tag="VVE42_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1471 a         1018 c          845 g         1420 t
ORIGIN      
        1 caattttatc tgatgattgc ccaaattttt caataataaa acgcataata ttatggagac
       61 acacaaaatc acctatatta tgcgttcttt taatatcaaa gactttttga caaacttctt
      121 tgatgtctaa ttactgcccc ctgcaggaat cgaacctgca actactcctt aggagggagt
      181 tgttatatcc attgaactaa gggagctaga taaaaactct gctgaaaagc agagtttttt
      241 agtcgaatta acgacggatt tctttgatac gagctgcttt accttgaaga gcacgcaagt
      301 agtacaattt cgcacgacgt actttaccgt aacgaacaac ttcgattttt tcaacacgtg
      361 gagtgtggat tgggaagata cgctcaacac ctacaccgtt agagatttta cgaactgtgt
      421 agttttctga gattccagca cctttacgtg cgataacaac accttcaaaa atctggatac
      481 gttcacggtt accttcgaca actttcgcgt gtacacgaac agtgtcacca ggacggaatg
      541 atgggatatc tgtacgaagt tgaccttcag tcaagctttg gattaatgga ttcattttat
      601 tctcctatct ttgtcaatct tgaggaaact tcctcagcgg ataaactgta tttttgtgcg
      661 tccatcacac acaagataca gtctaccaaa ttttcacaaa aaagtaaaga aatttatagc
      721 aaaattccta aaaaaatcgc aaccaaacca cctatgtaag tcaaagtcag gtaagaataa
      781 aagaccttct tatcacttag cagtctttga agttcgtcat tcaaggtcga aaaagttgtt
      841 aaaccgccac aaaatcctgt tgctagaata gcatatactt ccttagactc tacatgattg
      901 tagaatactc caattaaaaa acaacctaaa agattagcta tgagcgttcc aagtggcaat
      961 ttagaagctt gattgtagcg ggaaaagaaa taccgtacga gtgctcccaa gccacaggcg
     1021 attgcaagat agacgattac catttcttcc tccctagaaa ataagctaag agtaggcctc
     1081 caccgatgct caaaagcaaa tacagtatca aactaagata acgccctgta tcaagcagct
     1141 tcacagtatc aagcattagg ctggaaaaag ttgttaaacc tccgcaaaaa cccgtcccca
     1201 gtgctaaaat caagccctta ctagtcccct tatagaccag atagcccttg acaagataaa
     1261 tcaagcagaa aattcccaga tagttgacaa agagagtgcc ccaagggaaa tctggactgg
     1321 ctggtaacca agtggaaact aggtagcgga caagtccgcc caacatagca gctagaaaaa
     1381 tccctagcgg ataaaattgt tcttttttca tttgatattt tgatcctgat aatcacgcga
     1441 acatttgagt atgtccgaaa aagtcgcgac aatagtctcc tgatagcgct tgtcctctac
     1501 acgacttcta accttttcaa aaataactgc ttctctctta gtatctaaaa tcggtttccc
     1561 tgaagccttc ttataagcga caactccttc aactaagtgc attcgttctt ctaacagttt
     1621 gacaatttgg tcgtcgattt gatcgatttc ttgacgaata atatctaaat ccatacagtc
     1681 tcctccttta tttgaattat tgtatcaaaa agccatcaat taggctagta aaatcccaac
     1741 tctcgataag aaaaatgcac tgattgatta catcagcgca cttttatgct tatcctactt
     1801 tagcaaccaa ttcttctgcg aattgttcca agcgttcaat atcttcttcc tcggcagaaa
     1861 gatcaacttt aacacactct gaaccttttt ctgctcctgt tgacacaaag acacggtcaa
     1921 agtcatcaac agccttacag aattcatcgt agaaggtatc tcctgagccg accactccgt
     1981 agattttgcc attcaagttg agatctgcta ggtcttcgta gaagtccatc atctcatctg
     2041 gcaattcacc atcaccataa gtataggtcg caacgatagc gatgtctgct tccaagaagt
     2101 ctgaagcgtc aacagttgta cattcatcaa catcaacatc caagcccaag tcacgtaatt
     2161 tgtctgctac aatatctgca atttcttcgg tattaccggt catactggca aatacaattt
     2221 ttgctaatgc catatcgtcc tcctcaattt atctttctcc attatatcac atctttttca
     2281 ttttaaaaat aaaaattctt gtgctacaat gaaatgagaa agaattgagg ttatttatgg
     2341 agatttgcca acaaatttta gaaaaaatta aagaatacga caccattatc attcatcgtc
     2401 atatgaaacc agaccctgat gccttgggaa gtcaggtggg attgaaagcc ttgctagaac
     2461 atcatttccc agaaaaaacc atcaaagccg tcggttttga tgaaccaact cttacttgga
     2521 tggctgagat ggatcttgtt gaagataggg cctaccaagg cgcacttgtc atcgtctgtg
     2581 atacagccaa tactgctcgt atcgatgata agcgctatag tcaaggtgat tttctcatta
     2641 agattgacca ccatccaaat gatgatgtat acggtgacct gtcttgggtc gatactagtt
     2701 caagtagcgc tagcgagatg attaccctat ttgcccaaac aacccaacta gccttggcag
     2761 atcgcgatgc tgagttgctc tttgcaggaa ttgtcggtga tacaggtcgc ttcctctacc
     2821 cttctaccac tgcacggact cttcgcctgg ctgcttattt gagagaacat aactttgact
     2881 ttgcggctct cactcgcaaa atggacacta tgagctacaa aattgctaaa ctgcaaggct
     2941 acatctacga ccatctggaa gtggatgaaa atggtgctgc tcgcgttatc ctgagtcaag
     3001 aagtcttgaa acaatacaat gtaaccgatg ctgaaactgc agccattgtc ggtgcacctg
     3061 gacgcattga cagagtgagt ctctggggaa tttttgtcga acaggctgat ggccactacc
     3121 gcgttcgctt acgcagtaaa atccatccta tcaatgaaat tgccaaggag catgatggtg
     3181 gaggccaccc tctagcaagt ggtgctaatt cctatagcct agaagaaaac gaaatcatct
     3241 accaaaagtt aaaaaacttg cttaaaaact gataaaatac ttgccaaact tttcagaatc
     3301 tgatagacta gtatggtaac aatctatggc tcgcaaagag accatggcag aaaggaaata
     3361 ttgcaaaatg aaaaaagata tccatccaga atatcgccca gttgtcttca tggatacaac
     3421 tactggttac caattcctta gcggttcaac aaaacgctct aacgaaacag ttgagttcga
     3481 aggcgaaact tacccattga tccgtgtgga aatttcatca gactcacacc cattctacac
     3541 tggacgtcaa aagttcactc aagcagatgg acgcgtggat cgtttcaaca aaaaatacgg
     3601 tctcaaataa tgataagaga acagttttgg ctgttctttt ttgtttcttg aaatcaactg
     3661 ctgttttcat gttccagact catctgtagg ttcggtttcc atgctactag gcaggaagga
     3721 aatagctgtt tcaacacgtc cataatgagc tatactattg taacgaacca cactttcatt
     3781 gatggtccaa gtggaattca ttttcttaaa agcttctcgg actttttcca aatctttgga
     3841 ggcaatggcc tgctctaagg tttcaaaacg aggacttata ctcatctgct ttcacaaagc
     3901 attctagtcc atctccgatc cttgtatgac atcttgaagt tgattcatga tatcttccaa
     3961 agttcgaaag gccttattct taaatccacg tttacgaatc tctttccaca cttgttcaat
     4021 gggttcatct ctgatgtgta tggaggaata aaggtaaaac caatattagt cggaatcttt
     4081 aaggtacttg atttatgcca tatagcattg tccataacga gtaaaaggat aagcttgtga
     4141 aagctcttat aaaaaggcgt tcatccacac tcctttttat aagcctgaaa taaggcatca
     4201 attgtaacaa attctcctgc ctctgtagcc ttcaaatgac gggcaagaaa ggctttctct
     4261 tcctcaactg tcatatatgc atggttacga ccaccacgtg tttcttgaag gagagagtcg
     4321 agtccgaact cctcatattt tttacatttc gctaaatcgt tgtttgatta cagtctaaaa
     4381 gttctataat ctctttataa gatttgccca tcagacgaaa tataataaat tgaaactaga
     4441 atagtacacc tctacttcta aaacattgtt agaaatcgat ttgactgtcc tgattgattt
     4501 gtcctattct tgtttcattt tactatagaa cgatttgaag gcgtttatga tatttagctg
     4561 tactagagtt ttttaaaagt gttttgatgg tttggatttc ttctttaatt gatttcatat
     4621 tactattata taatgctttt tgattttagt ctggtataaa tattgctttc ctccaaaatg
     4681 gtcatagttt tactggcaaa tctaacatat cacggataaa ttaacaagtg atttctgaat
     4741 tactaaacat tttc
//
LOCUS       DAVGJL010000127         4609 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.128, whole genome shotgun sequence.
ACCESSION   DAVGJL010000127 DAVGJL010000000
VERSION     DAVGJL010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4609
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.128"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(<1..62)
                     /locus_tag="VVE42_001815"
     CDS             complement(<1..62)
                     /locus_tag="VVE42_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HEU3073964.1"
                     /translation="MLVFQMRNVDKTSTVLKQTK"
     gene            296..433
                     /locus_tag="VVE42_001816"
                     /pseudo
     CDS             296..433
                     /locus_tag="VVE42_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            complement(493..684)
                     /locus_tag="VVE42_001817"
     CDS             complement(493..684)
                     /locus_tag="VVE42_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEU3073965.1"
                     /translation="MTSKLTASTCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIE
                     IKQFKVTVITEPDAKTQSR"
     gene            1206..1376
                     /locus_tag="VVE42_001818"
     CDS             1206..1376
                     /locus_tag="VVE42_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HEU3073966.1"
                     /translation="MKYFEVELENPDEFLKLQTEDFVKANRLLLRKIIQSVTVYEENF
                     VISFKSGIELEV"
     gene            1762..2979
                     /gene="mef(A)"
                     /locus_tag="VVE42_001819"
     CDS             1762..2979
                     /gene="mef(A)"
                     /locus_tag="VVE42_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(A)"
                     /protein_id="HEU3073967.1"
                     /translation="MEKYNNWKRKFYAIWAGQAVSLITSAILQMAIIFYLTEKTGSAM
                     VLSMASLVGFLPYAILGPAIGVLVDRHDRKKIMIGADLIIAAAGAVLAIVAFCMELPV
                     WMIMIVLFIRSIGTAFHTPALNAVTPLLVPEEQLTKCAGYSQSLQSISYIVSPAVAAL
                     LYSVWDLNAIIAIDVLGAVIASITVAIVRIPKLGNQVQSLEPNFIREMKEGVVVLRQN
                     KGLFALLLLGTLYTFVYMPINALFPLISMEHFNGTPVHISITEISFAFGMLAGGLLLG
                     RLGGFEKHVLLITSSFFIMGTSLAVSGILPPNGFVIFVVCCAIMGLSVPFYSGVQTAL
                     FQEKIKPEYLGRVFSLIGSIMSLAMPIGLILSGFFADKIGVNHWFLLSGILIIGIAIV
                     CQMITEVRKLDLK"
     gene            3099..4562
                     /gene="msr(D)"
                     /locus_tag="VVE42_001820"
     CDS             3099..4562
                     /gene="msr(D)"
                     /locus_tag="VVE42_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(D)"
                     /protein_id="HEU3073968.1"
                     /translation="MELILKAKDIRVEFKGRDVLDINELEVYDYDRIGLVGANGAGKS
                     TLLRVLLGELTPPGCKMNRLGELAYIPQLDEVTLQEEKDFALVGKLGVEQLNIQTMSG
                     GEETRLKIAQALSAQVHGILADEPTSHLDREGIDFLIGQLKYFTGALLVISHDRYFLD
                     EIVDKIWELKDGKITEYWGNYSDYLRQKEEERKSQAAEYEQFIAERARLERAAEEKRK
                     QARKIEQKAKGSSKKKSTEDGGRLAHQKSIGSKEKKMYNAAKTLEHRIAALGKVEAPE
                     GIRRIRFRQSKALELHNPYPIVGAEINKVFGDKALFENASFQIPLGAKVALTGGNGIG
                     KTTLIQMILNHEEGISISPKAKIGYFAQNGYKYNSNQNVMEFMQKDCDYNISEIRSVL
                     ASMGFKQNDIGKSLSVLSGGEIIKLLLAKMLMGRYNILIMDEPSNFLDIPSLEALEIL
                     MKEYTGTIVFITHDKRLLENVADVVYEIRDKKINLKH"
BASE COUNT         1514 a          724 c          955 g         1416 t
ORIGIN      
        1 tttttagtct gtttcaaaac agtagatgtt ttatctacat tacgcatttg gaataccaac
       61 atgacgaatc cctccttctt aattacaaat ttttagcatc taatttaact tcaattccta
      121 ttatacaaaa ttttaagata atgcaccact tttttataat taaaacggtt gaaaactgta
      181 ccactaataa ctcacaatag agagatgtca ccgtcaagtt aaatgtacaa aataacagcg
      241 aaatttttaa atctatttct tatcgataca aattcctcgt aggcgctcgg gacccctacc
      301 tgctaataac ttggtaaaaa gcacccgacg attacagata gagaaataca tggatagctt
      361 tacagacaaa gaactatcct taatggaatc tttagccagc ggtatcaacg aagcaagaaa
      421 catcgaagac taattaaaag aatccataca taacggaaag agccgataaa atgagattgt
      481 attaatctca ttttatcggc tctgcgtctt tgcgtctggc tctgtaatca cagttacttt
      541 gaactgcttt atttcaatta aattttcttg tctgcatttc ggacaataga gggggaattt
      601 ttttaattca gtatcttccc ttatctttaa tcgtgtttta tttccacata caggacatgt
      661 tgaggcggta agtttgctag tcaaggagta aaacgacgaa gattagcatt tacttccgcc
      721 catgcgatag ctgtccgtga ttgacaagtg ctagcacgca gacagaacgg agatagcgaa
      781 ccgctgagtg tgtcgctctg ctcgtaaaag cttagaaacc tttgaacgaa agggataatg
      841 aaagccttga ttgcaaggct ttttgcttta tggtgggtaa gtatcagagt gagaaaattt
      901 ttggaatgag tagaagtgat agctagaaat tatcagtttc tatttccatt taccctgtgg
      961 gtacgtgttt gtttccattg acaaggagtt tgtgggaata gaaatgtacc caccttgttt
     1021 gaatcaagtg aagtgtagtt gaaggaaatc tgttgaaagc aatacttcat tttaccgaat
     1081 aagtaataat ttaggcaact tcaaatcgat taaaaaaaac tattttaaag gttaagagta
     1141 gacaaaaatt gtccactctt ttttgcaaac tcaatttatc aataaatgaa atgagggaat
     1201 gtaaaatgaa atattttgag gttgagttag aaaatcctga tgaattttta aaactacaaa
     1261 cagaagattt tgtgaaagct aatcgcttgc tactaaggaa gataatccag agcgttacag
     1321 tctatgaaga aaacttcgtc atatccttta aatctggcat cgaattggaa gtatgagtct
     1381 cattccataa cttttatatt gaacatatca tcttgttgtg ttatactata aattgatata
     1441 aacaaagatg taggaggaac cgaaactatg acagcctcaa tgcgtttaag ataagctggc
     1501 aataaaaaaa gcagaatcta tacccgatga taggcttttt tgttgtgctt atttatacga
     1561 tattgagcat tcattagtta cggtgaggat attggttatt taactatacc tttatttaac
     1621 tatgtcttta atatgaatgt ttccaaattg tatgtatgca gaccaaaagc cacattgtgg
     1681 ggtttggcct gcatttttta ttgcctagaa tgctattcaa aatagaaatt caagcaaaat
     1741 aatatgcagg agataatata aatggaaaaa tacaacaatt ggaaacgaaa attttatgca
     1801 atatgggcag ggcaagcagt atcattaatc actagtgcca tcctgcaaat ggcgattatt
     1861 ttttacctta cagaaaaaac aggatctgcg atggtcttgt ctatggcttc attagtaggt
     1921 tttttaccct atgcgatttt gggacctgcc attggtgtgc tagtggatcg tcatgatagg
     1981 aagaagataa tgattggtgc cgatttaatt atcgcagcag ctggtgcagt gcttgctatt
     2041 gttgcattct gtatggagct acctgtctgg atgattatga tagtattgtt tatccgtagc
     2101 attggaacag cttttcatac cccagcactc aatgcggtta caccactttt agtaccagaa
     2161 gaacagctaa cgaaatgcgc aggctatagt cagtctttgc agtctataag ctatattgtt
     2221 agtccggcag ttgcagcact cttatactcc gtttgggatt taaatgctat tattgccatc
     2281 gacgtattgg gtgctgtgat tgcatctatt acggtagcaa ttgtacgtat acctaagctg
     2341 ggtaatcaag tgcaaagttt agaaccaaat ttcataaggg agatgaaaga aggagttgtg
     2401 gttctgagac aaaacaaagg attgtttgcc ttattactct taggaacact atatactttt
     2461 gtttatatgc caatcaatgc actatttcct ttaataagca tggaacactt taatggaacg
     2521 cctgtgcata tttctattac ggaaatttcc tttgcatttg ggatgctagc aggaggctta
     2581 ttattaggaa gattaggggg cttcgaaaag catgtattac taataacaag ttcatttttt
     2641 ataatgggga ccagtttagc cgtttcggga atacttcctc caaatggatt tgtaatattc
     2701 gtagtttgct gtgcaataat ggggctttcg gtgccatttt atagcggtgt gcaaacagct
     2761 ctttttcagg agaaaattaa gcctgaatat ttaggacgtg tattttcttt gatcggaagt
     2821 atcatgtcac ttgctatgcc aattgggtta attctttctg gattctttgc tgataaaatc
     2881 ggtgtaaatc attggttttt actatcaggt attttaatta ttggcattgc tatagtttgc
     2941 caaatgataa ctgaggttag aaaattagat ttaaaataaa caatattgga ggaatattta
     3001 tgtatcttat tttcatgtaa ctcttcctgc taaaatcgca gggttttccc tgcatacaag
     3061 caaatgaaag catgcgatta tagacaggag gaaatgttat ggaattaata ttaaaagcaa
     3121 aagacattcg tgtggaattc aaaggacgcg atgttttaga tataaatgaa ttagaagtat
     3181 atgattatga ccgtattggt ttagtaggag caaatggtgc tggaaaaagc actttactca
     3241 gggtactttt aggagaatta actcccccag gatgtaaaat gaatcgtctg ggtgaacttg
     3301 cctatattcc ccagttggac gaagtaactc tgcaggagga aaaagatttt gcacttgtag
     3361 gcaagctagg tgttgagcaa ttaaatatac agactatgag cggtggtgaa gaaacaaggc
     3421 ttaaaatagc acaggcctta tcggcacagg ttcatggtat tttagcggat gaacctacga
     3481 gccatttaga ccgtgaagga attgattttc taataggaca gctaaaatat tttacaggtg
     3541 cactgttagt tattagccat gaccgctatt ttcttgatga aatagtagat aaaatatggg
     3601 aactgaaaga tggcaaaatc actgagtatt ggggaaacta ttctgattat cttcgtcaga
     3661 aagaggaaga acgtaagagc caagctgcag aatacgaaca atttattgcg gaacgtgccc
     3721 gattggaaag ggctgcggag gaaaagcgaa aacaggctcg taaaatagaa cagaaggcaa
     3781 aaggttcttc aaagaaaaaa agtactgaag acggagggcg tttagctcat caaaaatcaa
     3841 taggaagtaa ggaaaaaaag atgtataatg ctgctaaaac cctagagcac aggattgcgg
     3901 ccttaggaaa agtagaagct ccggaaggca ttcgcagaat tcgtttcagg caaagtaaag
     3961 cattggagct ccataatcca taccctatag tcggtgcaga aattaataaa gtatttgggg
     4021 ataaggctct gtttgaaaat gcatcttttc aaattccgtt aggagcaaaa gtggcgttaa
     4081 ctggtggtaa tggaatcgga aaaacaactt taatccaaat gatcttaaac catgaagaag
     4141 gaatttctat ttcgcctaag gcaaaaatag gttactttgc acagaatggt tacaagtaca
     4201 acagtaatca gaatgttatg gagtttatgc agaaggattg tgactacaat atatcagaaa
     4261 ttcgttcagt gctagcatct atggggttca aacagaacga tattggaaaa agtttatctg
     4321 ttttaagcgg tggagaaatt ataaaattgt tgcttgctaa aatgctcatg ggtagatata
     4381 acatcctaat aatggatgaa cccagtaact tccttgacat accaagttta gaggctttgg
     4441 aaatactaat gaaggagtac accggaacta tcgtgtttat cacccacgat aaacgattac
     4501 tcgaaaatgt agcagatgta gtttatgaaa ttagagataa gaaaataaat ctgaaacatt
     4561 aaatttaagg tagtcgctgg tcagtatagt ctgttctggt tggcgactc
//
LOCUS       DAVGJL010000128         4597 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.129, whole genome shotgun sequence.
ACCESSION   DAVGJL010000128 DAVGJL010000000
VERSION     DAVGJL010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4597
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.129"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            382..1577
                     /locus_tag="VVE42_001821"
                     /pseudo
     CDS             382..1577
                     /locus_tag="VVE42_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713014.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(1956..2741)
                     /locus_tag="VVE42_001822"
     CDS             complement(1956..2741)
                     /locus_tag="VVE42_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU3073969.1"
                     /translation="MKKYQRMHLIFIRQYLKQIMEYKADFLVGVVGVFLTQGLNMLFL
                     NILFQHIPLLDGWSFHQVAFIYGFSLIPKGIDHLFFDNLWALGQHLIRKGEFDKYLTR
                     PISPLFHILVETFQIDALGELLVGVLLLLMTITSLTWTWAKVFLFLISIPFATLIYTS
                     LKIVTASIAFWTKQSGAIIYIFYMFNDFAKYPIAIYHSFLRWLISFIIPFAFTAYYPA
                     SYFLKDKDGLFNIGGLILISLIFFTLSLKLWNKGLDAYESAGS"
     gene            complement(2743..3561)
                     /locus_tag="VVE42_001823"
     CDS             complement(2743..3561)
                     /locus_tag="VVE42_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEU3073970.1"
                     /translation="MTKLWKRYKPFVSAGIQELITYRVNFFLYRIGDVMGAFVAFYLW
                     KAVFDSSHQSLIQGFTLSDMTLYIIMSFVTNLLTKSDSSFMIGWEVKDGSIIMRLLRP
                     VHFAMSYLFTEIGSRWLVFVSVGLPFVILIAGLKLLSGESFLQIVLITTVYLLSLILA
                     FLINFFFNICFGFSAFVFKNLWGSNLLKNSLVAFLSGSLVPLTFFPKIIAELLSFLPF
                     SSLIYTPVMVIIEKYSMSQMIQALSLQLFWLFIMIALSQLIWKCVQNYITIQGG"
     gene            complement(3563..4555)
                     /locus_tag="VVE42_001824"
     CDS             complement(3563..4555)
                     /locus_tag="VVE42_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU3073971.1"
                     /translation="MKMITVEHLQKNFVKTIKEPGLKGAFRSFFHPKRQVFEAVKDLS
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQENRQGYVKDIGVV
                     FGQRTQLWWDLALQETYSVLKEIYDVPDAVFQKRMDFLNEVLDLKEFIQDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLG
                     DIEQLCDRIFMIDKGREIFDGTVNQLKKTFGKMKTLSFELHPGQDYIVSHFEGLSDIY
                     VTRQELSLDIQYDSSQYQTADIIQQTLSDFTIRDLKMTDANIEDIIRRFYRKEL"
BASE COUNT         1634 a          915 c          764 g         1284 t
ORIGIN      
        1 aagttactaa aaaaagattt tggaataaac tttttcatag cgataaaagc accgtacaag
       61 ctaggaaaat aataagtaat cgcccaatct gaccttctcc agaacttgat tattcgtcaa
      121 tcactacagt tgacaaacat actaagatta gtagtgagga aatctccaac gggagagagt
      181 actcattact ttttctttgt gttaaaatag tggtgtcttg ttaagtcata gagctctcta
      241 tggtgctaga cgtcgcttga caaactggca agaaacctgt tttaggaaga attttatcaa
      301 agtatgtggg atactagacg tcgagtattg gaaataaaat cactaaaaca aggagtcatt
      361 caagacaaag gggtaaacat gatgcggaca gttttcagaa tggatgtgag taaggcaagc
      421 tcagaagtcg ctattcttgt gaatggtgaa aaggttcatg gctataccat gcccaatgat
      481 gccatcggat ttagtcgtct tctagaggat ttgaaatagg taaatacacc tgagattatc
      541 ttcgaggcac aggggtctat tctcgtcttc aggcatttct ggaggacaat agctatgctt
      601 atatacggct aaatccttta aaactaagaa gcaactagac agtttgcgag tccgtaaaac
      661 caatcagatt gacgctgaaa agctagcttc gtcacaattc atccttaacc gcaaaccaac
      721 ttatgttcaa gaagaagtct atcaaaattt gcgtgacctc agtcgattct atcagaatct
      781 gacagaagac atcgttagga ctaagaatag cctgcacaag gtgcttcaaa ttgcctttcc
      841 agaaataaag aaccttctct ctactcctac aggggaacaa tattggaacc tcgtgaccat
      901 ctttccaacc aagcattgga ggctgaaatt atcagtagtt gagctaaaag aagccatcgt
      961 aattcaactt ctaaacggat ttctgagaac agagttggac gattggctga aaagttgagc
     1021 gagctggccg aacagtccta tcttgccatt tcaaaaacct ctcccatgac agaagaggtt
     1081 aagtatcatg cacaggaact gttgagactg acaaaccgca aggatcagga cttagcacaa
     1141 atgattgaat tggctgaacc attacctgaa tacgagattc tgctctctat tcctggtatc
     1201 gcagaaacaa cagctacaag cattattggc gaattggaga cattcgtcgc tttcagtctg
     1261 ccaaccaaat caatgccttt atcagtattg acctcagaca ctatgaatct ggcaatttcc
     1321 tagctcaaga acatatcacc aagcgtggaa atccttacgc gagaaaaatt ctctataagt
     1381 gcgtccacaa tattgcttca gctagtcata ccaatccttg tcatatcgcg gacttttatg
     1441 agaaacgaaa aagacaatcg cagacgactt caaccaagcc atatacgatt gtctctatac
     1501 atcgcctcat ccggacaatg tattacctca taacgcataa caaactttac gactacactt
     1561 taacccaaag tcattaaagt catctatgtc ctatcattgt aacaccttat tttttaatac
     1621 cagataagtg tcttttaagt gtgcccaaat ttcaaaaaac agctcgcaat tatcttctaa
     1681 acattccttt gatactcatt cagaaaacct tttgagctag aatatcgtat gtcccttaac
     1741 aaatggtatg tataaaagag ctaaaaaacc actcatgata tgaacgagtg gttatgactg
     1801 aaatattaca tacctttgta tcatttttgc acaatggtca aatttcttgt ttgaaaaact
     1861 acagtaagcc taatcaaagc tctcaatgta tcattaaaac ttccttccaa agaataggaa
     1921 gttctatcaa aattcttaat tagattttac ttcttctaag accctgcgct ctcatatgca
     1981 tccaaacctt tgttccataa ttttaatgat aaggtaaaaa atattaatga gataaggatc
     2041 aatccaccaa tattgaacaa tccatccttg tctttcaaga aatagctagc tgggtaatag
     2101 gctgtaaaag caaaaggaat gataaaacta atcaaccaac gtaaaaacga atggtaaatc
     2161 gcaatcgggt attttgcaaa atcattaaac atgtaaaaaa tataaattat tgccccagat
     2221 tgtttagtcc aaaaagcaat actagctgtt acaattttta aagaggtata aattaaggtt
     2281 gcaaagggaa tactgattaa aaagagaaaa acctttgccc aagtccaagt aagactagtt
     2341 attgtcatta acagcaataa gacgcctact aacaattctc caagagcatc aatttgaaac
     2401 gtttctacta aaatatggaa caaagggcta atgggtctag tcaaatactt atcaaattct
     2461 cctttacgaa ttaaatgttg tcctaaagcc cacagattat caaaaaagag atggtcaatt
     2521 cccttaggaa tgagagaaaa accataaata aaagcaacct gatgaaaact ccagccatca
     2581 agtaagggaa tatgttgaaa caaaatattt agaaacagca tgtttaaccc ttgtgtcaaa
     2641 aagacaccca caacaccaac taaaaaatca gccttatact ccataatctg tttaagatat
     2701 tgtcggataa aaatcaggtg catgcgttga tatttcttca ttctaacctc cttgaatagt
     2761 tatataattc tgtacgcact tccaaatcag ttgggataag gcaatcataa taaacagcca
     2821 gaataattgt agcgagagcg cctgaatcat ttgactcatg ctatactttt ctataataac
     2881 catcaccggg gtatagatta aagaagaaaa tggcaaaaaa gataatagtt cggcaataat
     2941 ttttggaaaa aatgtcagag gaaccaaact tccagataaa aaggcaacta aagaattttt
     3001 caaaagattt gatccccata agtttttaaa cacaaaagct gaaaaaccaa agcaaatatt
     3061 gaaaaaaaaa ttaatcaaaa aagctaagat taagctaaga aggtaaactg tggttattaa
     3121 aacaatttgt aagaaggact cacctgataa gagctttaga cctgcaatca gaattacaaa
     3181 tggtagacca acactaacaa aaactagcca tctagaccca atttctgtga aaagataaga
     3241 cattgcaaaa tgaactggac gaagcagtcg cataataata gagccatcct ttacttccca
     3301 tccaatcata aaagaactat ctgattttgt cagtagattc gtcacaaaac tcatgatgat
     3361 atataaggtc atatcagata aagtaaaacc ttgaatgaga gactggtgag atgaatcaaa
     3421 cacagctttc cacaagtaaa atgctacaaa ggcccccatc acatccccaa tgcgataaag
     3481 aaaaaaattg acacgatacg tgattaattc ttggattcca gcgctaacaa aaggcttata
     3541 gcgtttccat aatttagtca tcctaaagct cctttcgata gaagcgacga ataatatctt
     3601 caatattcgc atccgtcatc tttaaatcac ggatcgtaaa atcagacaaa gtttgttgaa
     3661 taatgtcagc cgtttgatac tgcgagctgt cgtactgaat atcaagtgac aattcctgtc
     3721 tcgtcacata gatatcagaa agtccttcaa aatgagagac aatataatcc tgcccaggat
     3781 gtagttcaaa agagagagtc ttcatcttcc cgaaagtctt tttcagttga ttcacagttc
     3841 catcaaagat ttctcggccc ttatcaatca taaaaatacg atcacataac tgttcaatat
     3901 cacctaaatc gtgggtcgtt aaaagtatgg tcgtctcttc ttcttgatta atctgtgtaa
     3961 tggcacgtcg aatattatcc ttgaccgaaa catctaaacc aatagtaggc tcatctagga
     4021 aaagaacttt cggattatgc aataaggagg ccgcaatatc cgccctcata cgttgaccta
     4081 gagatagagt tctgacagga tcttgaataa attccttcaa atctaaaact tcatttagaa
     4141 aatccatgcg tttttgaaag accgcatcag gaacatcata aatttccttt aaaaccgagt
     4201 aagtctcctg caaagccaaa tcccaccaaa gttgagttcg ctgcccaaaa actacaccaa
     4261 tatctttaac atagccttga cggttctctt gaggaatctt tccattgata cgacaaaaac
     4321 ctgatgttgg tttcaaaatc cccgtcaaca tcttgatagt tgttgatttc cccgctccat
     4381 ttgcgccaat aaagcctaaa atttgtcctt tgggcacctc aaatgataaa tctttaacag
     4441 cttcaaagac ttgtctcttg ggatgaaaaa aagagcgaaa agctcccttt aagccaggtt
     4501 cctttattgt tttcacaaaa tttttctgaa gatgctctac tgttatcatt tttataaatc
     4561 actcctttcg tttcactcaa gttataatat caacaaa
//
LOCUS       DAVGJL010000129         4595 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.130, whole genome shotgun sequence.
ACCESSION   DAVGJL010000129 DAVGJL010000000
VERSION     DAVGJL010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4595
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.130"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            543..752
                     /locus_tag="VVE42_001825"
     CDS             543..752
                     /locus_tag="VVE42_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU3073972.1"
                     /translation="MVKNLKLKLARVELDLTQGQLADAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
     gene            742..1230
                     /locus_tag="VVE42_001826"
     CDS             742..1230
                     /locus_tag="VVE42_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073973.1"
                     /translation="MKNRFYYYQLLDEREEQLFNKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYSRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYIIYLPWIFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            1346..2806
                     /locus_tag="VVE42_001827"
     CDS             1346..2806
                     /locus_tag="VVE42_001827"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEU3073974.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIHNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEDLRFSDSDIAYLESLGYHGAFLDYLRNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGEQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DENGQMRNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISDMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            2803..3627
                     /gene="nadE"
                     /locus_tag="VVE42_001828"
     CDS             2803..3627
                     /gene="nadE"
                     /locus_tag="VVE42_001828"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEU3073975.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFV
                     LGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            4026..4577
                     /locus_tag="VVE42_001829"
     CDS             4026..4577
                     /locus_tag="VVE42_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU3073976.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSAENLTYVTWD
                     PHPDIEITRNSIRNWVASYTNLNYYKWAICLKENPEQVIGDISIVKIDEADLSCEIGY
                     VLGKAYWGNGMMTETLKAILDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
BASE COUNT         1372 a          859 c         1045 g         1319 t
ORIGIN      
        1 tttgtttatc aaagcaattg aacttcttcc tttttattct ttacctgtat ttgtgacttc
       61 aaatacgatg tctattagtc agtatcttgt gctaatcact acaatcattt tattgtactt
      121 tttcactctc aagaaaagca agaagtattc attttagttt catttagtat tattttgcat
      181 acctaaaata cagtaaaaaa atcagtcatc ttggtatgct cctgctttca ctattcaaca
      241 cgtttttgac ttatactagg ctcattttca aaagcattat ataatagtga tatgaaacca
      301 actaaactaa acaagaaata taagcaataa aaactcgttt aaaagctcct actaaagcta
      361 atactaaata aaaataaaag agtaaactag gaagtttatt tcaaacaacc taaaatactg
      421 attttcggct gaagataata ctggagtgca aattaatggg gttataataa atagctgaca
      481 gcttgtgtta gtttggaatt ttttaagagt agatgaatat taaaattata aggaggacga
      541 aggtggttaa aaatttaaaa ttaaaattag ctcgggtaga gcttgattta actcaaggtc
      601 aactagcaga tgctgtcggg gtgacgcgcc agactattgg tttgatagag gcggggaaat
      661 acaatcccag tctctcgctc tgccagtcca tttgcagatg tttagggaaa accctagacc
      721 aactattttg ggaggaagaa gatgaaaaat agattttatt attatcaatt actagacgaa
      781 agagaagaac aactgttcaa taaagcgggc tctgaaagtt tctatatctg catcgctttg
      841 tcgctcctat cttatatcat ttcagtatta gcaccaagcc tttttaattc taatatgctg
      901 ctaatcgtta tcatcatagg gacattttac tttttcaatc gtgcccgtta tctgggagtg
      961 acctactata gtcgttttca ttttacgatt ttgggttgtt ttttcctaac cttggctatt
     1021 acggctcttt tgatgttgca gaattatcaa ttcaacatag aaatttatca gcacaatcct
     1081 ttgaatttta aatacctgtc tgcttgggtc attacttata tcatttacct tccgtggatc
     1141 tttattggca atcttggtct taagagctat ggcgaatggg ctcagaaaaa atttgaacaa
     1201 gatatggatg aattggagag tggagaatag cttgttactc ttttctcaat ccagctaaaa
     1261 tgtgatataa tagtactaat ttattggaat acatgaaagt tcttgaaaat tttcatgggt
     1321 ttctagctaa ggaagtagga aaagtatgta tccagatgat agtttgacat tgcacacgga
     1381 cttgtaccag atcaacatga tgcaggttta ctttgaccaa gggattcaca ataagaaggc
     1441 ggtctttgag gtgtatttcc gccaacagcc ttttaagaac ggctatgcgg tttttgcagg
     1501 tttggaaaga attgtgaact atcttgaaga cttgcgtttt tcagatagtg atatagccta
     1561 tttggagtcg cttggttatc atggggcgtt cttggattac cttcgcaatt tcaagttgga
     1621 gttgaccgtt cgttctgccc aagaagggga tttggttttt gctaatgaac cgattgtgca
     1681 ggtggaagga cctctagccc aatgtcagtt ggtcgaaacg gctcttttga acatcgtcaa
     1741 ctaccagacc ttggtggcga cgaaggcagc tcgtattcgt tcggttatcg aagatgaacc
     1801 cttgatggag tttgggacac gtcgggctca agaaatggat gcggccatct ggggaacacg
     1861 cgcagcggtg attggtggcg ccaatggaac cagcaacgtg cgtgcgggta agctctttga
     1921 cattcctgtt ttgggaaccc atgcccatgc cttggtacag gtttatggca atgactatga
     1981 agctttcaag gcttacgctg cgacccacaa aaattgtgtc tttcttgtgg atacctatga
     2041 cacccttcgc atcggtgtac cagctgccat tcaggtggcg cgtgagctgg gtgaacagat
     2101 taactttatg ggtgtgcgga ttgactctgg ggatattgcc tacatttcta agaaagtccg
     2161 tcagcaactg gacgaggctg gatttacaga ggctaagatt tatgcttcta atgatttgga
     2221 cgaaaatact atcctcaacc tcaagatgca aaaggccaag attgatgtct ggggcgtggg
     2281 taccaagctg attacagctt atgaccagcc agctcttggt gcagtttata agattgtggc
     2341 catcgaagat gaaaacggcc agatgcgcaa tacgattaag ctgtctaata atgcggaaaa
     2401 agtgtcgacg ccaggtaaga agcaggtgtg gcgcattacc agtcgtgaaa aaggtaagtc
     2461 agaaggtgat tacatcactt atgatggtgt ggatattagc gacatgacag aaatcaagat
     2521 gttccatccg acctatacat acatcaagaa gacggttcgt aattttgatg ccgttcctct
     2581 cttggtggat atcttcaaag aaggaatatt agtttacaac ttgcctagtt tgactgacat
     2641 tcaggattat gcccgtaaag aatttgacaa gttgtgggat gagtataagc gtgtgctcaa
     2701 tccgcagcac tatccagtgg atttggcgcg tgatgtatgg caagataaga tggacttgat
     2761 tgataagatg cgcaaggaag cccttggtga aggagaagaa gaatgagttt gcaagaaacg
     2821 attatccaag agctgggtgt caaaccagtg attgatgccc aggaagaaat ccgtcgttct
     2881 attgatttct taaaaagata tctgaaaaaa catcccttcc taaaaacctt tgtactaggg
     2941 atttctgggg gacaagactc aaccttggca ggacgtttgg cgcaattagc tatggaagaa
     3001 ctgcgagctg aaacgggaga cgacagctac aaatttatcg ctgtccgctt gccatacgga
     3061 gtgcaagctg atgaagcaga tgctcaaaaa gccctagcct tcatccagcc agatgtcagc
     3121 ttggttgtga atatcaagga atcagctgat gccatgacag ctgcagttga agcgacaggt
     3181 agtcctgttt cagacttcaa caaggggaat atcaaggcac gttgccgtat gattgctcag
     3241 tatgcccttg ctggttccca tagcggagcg gtcattggaa cagaccacgc cgcggaaaat
     3301 atcacaggtt tctttaccaa gtttggtgac ggcggtgcgg atattctccc tctttaccgc
     3361 ctcaataaac gccaaggaaa acagctcttg cagaaacttg gagcagagcc agccctttat
     3421 gaaaaaatcc caacggcaga cctagaagaa gataaaccag gcctagctga cgaagtcgca
     3481 cttggagtca cctacgcaga gattgacgac tacctagaag gcaaaacaat cagcccagaa
     3541 gctcaagcga ccattgaaaa ctggtggcac aaaggccaac acaaacgcca cttacccatc
     3601 accgtatttg atgacttttg ggagtaaaaa cctccattgg aggtttttac cctgagccta
     3661 gaaatgagaa agcgaagaag gtccggggga cctttttagc ttcttgccct gaaattaaaa
     3721 agcaagaaaa acctccactg gaggttttca gcctctcatc ttgaaataag aaagtgagag
     3781 aaggtccggg ggatcttgaa cctcgagttt agaaataaga aaatgaggca gattcggtaa
     3841 ctcgaagagt tcgatttcat cgtcttaccc ctgcaacgat gactaggttt gaaaaagctt
     3901 gctagagcgc atttcaaacc aggcagcgac tgcgtcaaga aattagaaga taaactcgtt
     3961 ttctagctgt tactgagttg agccttttta ctacgagcat agaaataagg aagtgaggta
     4021 gtatcatgaa atctatcggt acgcaaatat tacagacaga acgtttgatt ttaagaagat
     4081 ttgtggagag tgatgcagaa gccatgtttc aaaattgggc ttcatccgct gagaatctga
     4141 cctatgttac ctgggatccc catcctgata tcgaaatcac tcgaaactcg attcgcaatt
     4201 gggttgcttc ctatactaat ctcaactatt ataaatgggc catttgtcta aaagaaaacc
     4261 cagagcaagt gataggagat atcagcatcg ttaagataga cgaggctgat ttaagctgtg
     4321 aaattggcta tgtgttaggc aaggcttact ggggaaatgg tatgatgaca gagactttga
     4381 aagctatctt ggacttttgt tttactcaag caggttttca aaaggtcaga gcacgttatg
     4441 ccagtctcaa cccagcttca ggtcgtgtca tggaaaaggc tggaatgtcc tatctacaaa
     4501 ccattgttaa tggtgtagag agaaaaggct atcttgcgga tcttatttat tatggtataa
     4561 gtagggaaga atgttgaatt ctattttctg tttct
//
LOCUS       DAVGJL010000130         4563 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.131, whole genome shotgun sequence.
ACCESSION   DAVGJL010000130 DAVGJL010000000
VERSION     DAVGJL010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4563
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.131"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            235..399
                     /locus_tag="VVE42_001830"
     CDS             235..399
                     /locus_tag="VVE42_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU3073977.1"
                     /translation="MGIIANVFAGLVGASAGQSLLGSWGPSIAGMALLPSIVGAAIVI
                     TVVSFFTGRK"
     gene            483..1049
                     /gene="amaP"
                     /locus_tag="VVE42_001831"
     CDS             483..1049
                     /gene="amaP"
                     /locus_tag="VVE42_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEU3073978.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNS
                     VVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVSDHRLIKNPTVHVNLRKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            1061..1231
                     /locus_tag="VVE42_001832"
     CDS             1061..1231
                     /locus_tag="VVE42_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEU3073979.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     AGLYIEKNYIDK"
     gene            1269..1877
                     /locus_tag="VVE42_001833"
     CDS             1269..1877
                     /locus_tag="VVE42_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU3073980.1"
                     /translation="MSNEKNTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGLSLEN
                     VSGLLGIDGGFFSNLKEKIVNSDDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSE
                     IREIVSSEVAKMTDLEIVEINVNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQ
                     FEKAKSGLGSGFSTVQEKVSEGVEAVKGAANGVVSHENTRVN"
     gene            1908..2111
                     /locus_tag="VVE42_001834"
     CDS             1908..2111
                     /locus_tag="VVE42_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEU3073981.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLSGDDK"
     gene            complement(2221..2493)
                     /locus_tag="VVE42_001835"
                     /pseudo
     CDS             complement(2221..2493)
                     /locus_tag="VVE42_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547411.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2533..2979)
                     /locus_tag="VVE42_001836"
     CDS             complement(2533..2979)
                     /locus_tag="VVE42_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU3073982.1"
                     /translation="MTIRFEEKVSIENAQFVCQWSNSLGKSFQEQWMGPRIPFLLTLQ
                     ALEGVFSIFDEQEFVGLIQKIRLEDSNLHIGRFFINPQKQEQDLGSQALRKFVSLAFE
                     NEDIDSISLNVFEANQRAQNLYQKEGFEIVQMVEAPVRKYIMKKGR"
     gene            3049..3708
                     /locus_tag="VVE42_001837"
     CDS             3049..3708
                     /locus_tag="VVE42_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEU3073983.1"
                     /translation="MIDFYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTRLR
                     PLDLIPILSQVFNRFRCRYCKVRYPVWYTLFELVLGLLFLLYSWELLSLGQVVLITAG
                     LTLGIYDFHHQEYPLLVWMTFHLILIASSGWNLVMVSFLALGILAHFIDIRMGAGDFL
                     FLASCALVFSVTELLILIQFASATGILAFLLQKKKERLPFVPFLLLAACVIIFGKLLL
                     V"
     gene            complement(3760..>4563)
                     /locus_tag="VVE42_001838"
     CDS             complement(3760..>4563)
                     /locus_tag="VVE42_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEU3073984.1"
                     /translation="HDLSNVVSESYHKGESIEELALYAREKLGISKDNHDLLYKLERS
                     GIYIVERLINGQADAYSAWSKLGRPYIVLGTNKSSVRRNFDLAHELGHILLHKYKDMN
                     EDGDRLEQEANYFASCFLLPKEEFLVKFEERVGKRVSNPDSYILLKSDLNVSIQALEY
                     RAFKLGLLTPKQHSYFYRQIAQKGYKMIEPLDDQIFVKKPSKVKSILDVVLSNHLVSL
                     ATIMSKQSIRLQFISEIFSVEMKFFDQYQEDRRTDRFDNIIPLYKRNNL"
BASE COUNT         1427 a          820 c          797 g         1519 t
ORIGIN      
        1 taattttaaa aattatataa tagcaacaat taaagaattt gattttttaa aattatataa
       61 taataacaat cgaaataatt gacttttcta tattaaagtt atataatagt aataatcaaa
      121 gaaattgatt ttttgatatt aaaataaaaa aggagggtag gcagtgttgt gatcaattat
      181 tgctggaggt cttattggtc tcttggcagg taaaatcact aaaaaagtag ttctatggga
      241 atcatcgcaa atgtattcgc tggtttagtc ggggcatctg caggacaatc tcttttaggt
      301 agttgggggc catcaatcgc tggaatggct ttgctcccat ctattgtagg tgcagcgatt
      361 gtgattactg tagtgtcatt ctttacaggt agaaagtaaa cttttcacca gtaaagttag
      421 caaactattt ttaaatcaat gacgggaaaa atagtttaaa tgttaaatcg aaaggattgt
      481 atatgtcaaa agcaaagaaa atatgtttca ttattttctg tattttaatc ttgacaattt
      541 tccttcctgt tttgatagat tatcatcaag ttagtgatct aggtattcat ctacttagct
      601 ggagacagaa ctccgtagtt gaattctatc ttgctagata tgtcttttgg gggacagtgg
      661 ttctatcaac tttagtttta ttatccattt tagttgtgat gttttatcct aaacgttact
      721 tggaaatcca acttgaaact aaaaacgata cattaaaatt aaagaattcg gcaatcgaag
      781 gttttgttag aagtttggtg agtgatcata gattgatcaa gaacccaact gttcatgtaa
      841 atttacgaaa aaataaatgt ttcgttcatg tagaaggtaa aattcttcct tcagacaaca
      901 tcgctgacag atgccaaata attcaaaatg aaataactaa tggattgaag cagttttttg
      961 gtattgagcg tcaagtaaaa cttgaagttg cagtaaaaaa ttaccaacca aaacctcaaa
     1021 acaaaaagac tgttagtcgt gtgaagtaag gaagtaaaaa atggaatggc ttaaacaata
     1081 tcgatatcca attatcgctg gtctcatagg cgtatttctg gcttgtttga ttgtatcctt
     1141 tggcttcttc aaaacaatat ttgtattgat tttaggagca ctgggagttg cagctggatt
     1201 atatatcgaa aaaaactata tagataaata aaaaataaaa attactaatt taattaaagg
     1261 agtttcatat gtcaaacgaa aaaaacacaa acactaacgt agaaaagaaa gatgctactg
     1321 ttgtagctca cgaaatcaaa ggggaactta cttacgaaga taaagttatc caaaaaatca
     1381 ttggtctttc actagaaaac gtttcaggtc ttttgggaat cgatggtggt ttcttctcaa
     1441 atcttaaaga aaaaatcgtt aacagcgatg acgtaacaag tggtgttaac gtagaagttg
     1501 gtaaaacaca agttgcagtt gacttaaacg ttattgttga gtaccaaaaa aatgttccag
     1561 ctttatattc agaaatcaga gaaatcgtat cttcagaagt tgctaaaatg actgacttgg
     1621 aaattgttga aatcaacgta aacgttgtcg acatcaaaac taaagaacag catgaagcag
     1681 actcagtaag ccttcaagat cgcgtatctg acgttgctga atcaacagga gaattcactt
     1741 cagaacaatt cgaaaaagct aaatctggtc ttggatctgg tttctcaact gttcaagaaa
     1801 aagttagcga aggtgtagaa gctgttaaag gtgcagcaaa tggtgtagta tctcacgaaa
     1861 acactcgtgt aaactaagat aaaataaata taacaggaga aattatcatg tcagtagaag
     1921 aaaaattaaa tcaagctaaa ggttctatta aagaaggtgt tgggaaagcc atcggtgatg
     1981 aaaaaatgga aaaagaagga gcggctgaaa aagttgtttc taaagtaaaa gaagtcgccg
     2041 aagatgccaa agacgctgta gaaggtgctg tagaaggtgt taaaaacatg ttgagtggcg
     2101 acgataaata aggttaaaag ttactttatc tttttagtaa cattagtcaa aagagtctga
     2161 gtcaagatga ttctcagaaa acaaaaagct agagattccc aattgcggaa ctctagcttt
     2221 ttaattttgc ctctttctct tattatattt cagcaggttg ttggccatga gtacgaatcc
     2281 catgtcaatt ctcacttgac gcttacctct cagatgacat ctcttataac ccaaacaaac
     2341 ctttatctgc ccaaagacag atttcatatc aatcttacgt ttagcgaaaa tttgtctacc
     2401 cttggaagat aaaagtgcct gatattcttt agtttttaaa cactggtaac gttcattcat
     2461 atacagtccc ttttgagggg ctgattcagg ttcataatcg cagtcaacat tgatttcaag
     2521 gctgtttgct ttctatctcc cctttttcat gatatatttt cgtacaggtg cttcaaccat
     2581 ttgaacgatt tcaaatcctt ctttttggta aagattctga gctctttgat ttgcctcgaa
     2641 gacatttaga gaaatactat ctatatcttc attttcaaag gccaaactaa caaatttcct
     2701 taaagcctgg ctacctaagt cttgctcctg tttctggggg ttgataaaaa atctcccgat
     2761 atgaagattg ctgtcttcta gcctgatttt ctggataagt cccacaaact cttgttcatc
     2821 aaagattgaa aagactcctt ccaaggcttg aagggtcagt agaaaaggaa tccttggtcc
     2881 catccattgt tcttgaaagg atttgcctag ggagttggac cactggcata caaattgagc
     2941 gttttctata ctcacctttt cttcaaaacg aattgtcatc ttttcctcac catcttatct
     3001 atgtttctcc attatactat ttctcccatt ttttacgaat agataagtat gattgatttt
     3061 tatttttttc tcgtcgggag cattctcgct tcctttcttg gtttggtcat tgaccgtttt
     3121 ccagagcaat ccattatcag ttcagccagt cactgcgatt cctgtcagac tcgcttgcgt
     3181 cccttagatt tgattccgat tctctcacag gtcttcaatc gctttcgctg tcgctactgc
     3241 aaagttcgct atcctgtctg gtataccctc tttgaattag tcttaggact cctctttctg
     3301 ctttactctt gggaattgct ttccttgggg caagtcgtcc taatcaccgc tggtttgacc
     3361 ttgggtatct acgactttca ccatcaggaa tatcccttac tggtctggat gactttccac
     3421 ctaatcctaa tagcttcctc tggctggaat ctggtcatgg tctccttcct tgctcttgga
     3481 attttggctc attttatcga tatccgcatg ggcgcaggag atttcctctt tttagcttct
     3541 tgtgctctcg tctttagcgt aacggagtta ctgatcttga ttcagttcgc ttctgcgacg
     3601 ggtatcctgg cctttctcct gcaaaagaaa aaggaaagac ttcctttcgt gcctttcctc
     3661 ttacttgctg cttgtgtgat tatttttggt aagctactgc ttgtctgata aaatccaatt
     3721 tctgccatat gtccttcatg aaattatttc acagttaaat tataaattat ttcttttgta
     3781 caaagggata atgttatcaa atcgatctgt tcttctatct tcttgatact gatcaaaaaa
     3841 tttcatttcg actgaaaata tttcgcttat aaactgtaaa cgaatacttt gtttagacat
     3901 tatagtcgct agactgacta gatgattact caaaacgacg tccagaatac tctttacttt
     3961 gcttggtttt ttaacaaaaa tttgatcatc caaaggttca atcattttgt aacctttttg
     4021 cgcaatttga cgataaaagt aagaatgttg ctttggagtc aataatccta acttaaaagc
     4081 tcgatactct aaagcctgta tcgaaacatt caaatccgac ttcaataaaa tataactatc
     4141 aggattgctg acacgcttgc caaccctctc ttcaaatttg actaaaaact cttcttttgg
     4201 caataaaaaa catgatgcaa aataatttgc ttcttgctcc aaacgatcgc catcttcatt
     4261 catatcttta tatttatgta aaagaatatg tcctagctca tgagctaaat caaaatttcg
     4321 acgtacagat gatttattcg ttcccaacac aatataaggt cttcccaatt ttgaccacgc
     4381 gctataagca tcagcttggc cattaattaa tcgttccacg atatagatgc ctgaacgttc
     4441 taacttataa agcaaatcat gattatcttt tgaaatacct aatttttccc tggcataaag
     4501 agccaattcc tcaatggatt ctcccttatg ataagattca ctcactacat tacttaggtc
     4561 atg
//
LOCUS       DAVGJL010000131         4518 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.132, whole genome shotgun sequence.
ACCESSION   DAVGJL010000131 DAVGJL010000000
VERSION     DAVGJL010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4518
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.132"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            68..1390
                     /locus_tag="VVE42_001839"
     CDS             68..1390
                     /locus_tag="VVE42_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU3073985.1"
                     /translation="MNNILAFLETKVAPFGEKVGNQRHLKAIREGFMMAMPLILVGSL
                     FLILISWPQEDFTNWLNSVGLLSILTTMNQSTVAIISLVACFGIAYRLSEGYGTDGPS
                     AGIIALSSFVLMAPRFSSMVYDKNGEQVKQLFGGAIPFSSLNASSLFMAITIGLVTAE
                     IYRMFIQRGITIKMPSGVPDVVSKSFSALLPGFTTFVLWALVLKGLEAAGVAGGLNGL
                     LGAIVGTPLKLIAGTLPGMILCVIVNSFFWFCGVNGGQVLNAFVDPVWLQFTTENQEA
                     VAAGQTLQHIITLPFKDLFVFIGGGGATIGLAICLFLFSKSRANKTLGKLAIIPSIFN
                     INTAILFTFPTVLNPIMLIPFIATPTINALITYVSMAVGLVPYTTGVILPWTMPPIIG
                     GFLATGASWRGALLQVVLILVSVAIYYPFFKIADKRNLEKEKATVGGK"
     gene            1392..3278
                     /locus_tag="VVE42_001840"
     CDS             1392..3278
                     /locus_tag="VVE42_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HEU3073986.1"
                     /translation="MVIRVFDQQKNTYSSFALEELSYYMNRVFKTNIELVEEKEADIF
                     VGLVNKEDRKDHVLISLDKGKGRIESNTIVGLLIGIYRMFHEFGVVYTRPGRGHDFVP
                     ELRFEDFLDKQLSIDETASYYHRGVCIEGADSFENILDFIDWLPKIGMNSFFIQFENP
                     YSFLKRWYEHEFNPYLNKEQFSNELVQELSDRLDKELQKRGLIHHRVGHGWTGEVLGY
                     SSKFGWESGLSISEEKKPYVAEINGKRELFNTAPILTSLDFSNPDVADKMVEIIKDYA
                     KKRPDVNYLHVWLSDARNNICECENCRQELVSDQYIRILNQLDRALTSEGLDTKICFL
                     LYHELLWAPQKEKLDNPERFTMMFAPITRTFEMSYADVDFDNSIPTPKPYMRNKIILP
                     NSLEENLSYLFEWQKAFKGDSFVYDYPLGRAHYGDLGYMKISQTIYRDVSYLSNLHLN
                     GYISCQELRAGFPHNFPNYVMGQMLWKKTRSYEELIEEYFSALYGENWQSVVEYLEKL
                     SIYSSCDYFNAIGSRQNDVLANHYYIAYNLADNFLPIIEENISKLLNSQKDEWKQLSY
                     HREYVVKMAKALYLQATGKTRQAQDEWRNVLNYIRGHELLFQSNLDVYRVIEVAKNYA
                     GFHL"
     gene            3446..3790
                     /locus_tag="VVE42_001841"
     CDS             3446..3790
                     /locus_tag="VVE42_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU3073987.1"
                     /translation="MKSKEEINMLGFTIVAYAGDARSDLMDALAFARDGYFEQARELV
                     ESANDSIVSAHREQTNLLAEEAYGDNFEVSFIMIHGQDTLMTTMLLYDQVKFFIDEYE
                     RIRKIEEHIGLQ"
     gene            3800..>4518
                     /locus_tag="VVE42_001842"
     CDS             3800..>4518
                     /locus_tag="VVE42_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEU3073988.1"
                     /translation="MENLQFKVLPKEFLLGAATAAYQVEGATRVDGKGINMWDVYLQE
                     NSPFLPDPASDFYYRYEEDIALAAEHGLQALRLSISWVRIFPDIDGDANVLAVHYYHR
                     VFQSCLKHNVIPFVSLHHFDSPQKMLETGDWLNRENIDRFIRYARFCFQEFTEVKHWF
                     TINELMSLAAGQYIGGQFPPNHHFQLSEAIQANHNMLLAHALAVLEFHQLGIEGKVGC
                     IHALKLGYPIDGQKENILAAKR"
BASE COUNT         1383 a          651 c          976 g         1508 t
ORIGIN      
        1 tatattgaca acgctttctg ttagtggtat atttaattta taaaaaataa aaaaaggagt
       61 tccggtgatg aacaatattt tagcgttttt agaaacaaaa gtcgctccgt ttggtgaaaa
      121 agttggcaac caacggcatt tgaaagctat tcgtgaagga tttatgatgg caatgccttt
      181 gattttagtc ggctctttat ttcttattct aatcagttgg cctcaagagg attttacaaa
      241 ttggctgaat agtgttggat tgctaagtat cttgacaact atgaatcagt caacagtagc
      301 gattatctcc ttggtcgctt gtttcggtat tgcctacagg ttgtcggaag gatatggtac
      361 agatggtccg tcggcaggga tcatagcctt atccagtttt gtattgatgg cacctcgttt
      421 ttcgagtatg gtttatgata aaaatgggga gcaggtcaag cagttatttg gcggcgcaat
      481 accattttct agcctgaatg catcttcttt gtttatggcg attactattg gattggttac
      541 agcagagatt tatcgtatgt ttatccagcg cggaattacg ataaaaatgc caagtggtgt
      601 cccagatgtg gtaagtaaat cattttcagc tcttttacct ggctttacta cttttgtttt
      661 gtgggctctg gtcttaaaag gtcttgaagc ggcaggagtt gcaggaggtc tcaacggact
      721 cctaggtgca attgttggaa caccgcttaa gttaattgca ggaacgcttc caggtatgat
      781 tctatgtgtt attgtaaact cattcttttg gttctgtgga gttaatgggg gacaagtttt
      841 aaatgctttt gtagacccag tttggttaca atttactaca gaaaaccaag aagctgtggc
      901 tgcaggacaa acactccaac acattattac attaccgttt aaagatttat ttgtatttat
      961 tggtggcggt ggagcgacta ttggtcttgc gatttgtctc ttcctattta gtaagagtcg
     1021 tgcgaataaa acattaggta agctagctat tataccgtct atttttaata tcaatacagc
     1081 tattctattt acgtttccaa cagttttaaa tccgattatg ctgattccgt ttattgctac
     1141 tcctacaatc aatgccttga ttacctatgt atcaatggct gtaggattag taccctatac
     1201 aacaggtgta atccttccgt ggacaatgcc accgattata ggaggcttcc ttgcaacagg
     1261 ggctagttgg cgaggagctc tattacaagt tgttttgatt ttggtttctg tagcaattta
     1321 ttatccattc ttcaaaattg cagataaacg caatcttgaa aaagaaaaag ctactgttgg
     1381 agggaaataa gatggttatc agagtatttg atcaacagaa aaatacttat tctagctttg
     1441 ccttagagga attaagttac tatatgaatc gggtctttaa gactaacata gagcttgtcg
     1501 aggagaagga agcggatatt tttgtaggat tagtcaataa agaggacaga aaagaccatg
     1561 ttcttatctc attagacaag ggtaagggga gaattgagtc taatacaatt gtaggtttac
     1621 ttattggaat ttaccgaatg tttcatgaat ttggggttgt gtatactaga ccagggcgcg
     1681 gacatgactt tgttccagag ttacgatttg aagatttttt agataaacag ctatctatag
     1741 atgaaacagc cagttactat cataggggag tatgtataga gggagcggat tcatttgaaa
     1801 atatactaga tttcattgat tggctaccta agattgggat gaacagtttt ttcatccagt
     1861 ttgaaaatcc ttactctttt ttgaaacgtt ggtatgaaca tgaatttaat ccatatctaa
     1921 ataaagaaca attttcaaat gaattagtac aagaattgag tgataggttg gataaagaat
     1981 tgcaaaaaag aggtcttatt catcatcgtg ttggtcatgg atggacaggt gaagttttag
     2041 gttactcttc aaaatttggc tgggaatcag gtcttagtat ttcagaggag aagaaaccct
     2101 atgtcgctga aataaacggg aaacgagaat tgtttaatac ggctccgatt ttaaccagcc
     2161 tggatttttc aaatccagat gtagctgata agatggtaga aattatcaag gattatgcca
     2221 agaaaagacc tgatgttaac tacttacatg tatggttgtc ggatgctcgt aataatattt
     2281 gtgaatgcga aaactgtaga caagaattgg tttcggatca gtatattcgt attctcaatc
     2341 aattggatag ggctttaacg agtgagggat tagatacaaa gatttgtttt ctgctttatc
     2401 atgagttgtt atgggcacct cagaaagaaa aattagataa tcctgaacgc tttaccatga
     2461 tgtttgcacc gattacaaga acatttgaaa tgagttatgc agatgtagat tttgataatt
     2521 ccatacctac gcctaaacct tatatgcgta ataaaattat acttccgaat tctcttgagg
     2581 aaaatttatc ttatcttttt gagtggcaaa aagcatttaa aggagatagt ttcgtatatg
     2641 actatccttt agggcgtgct cattatggcg atttaggcta tatgaaaatt agtcaaacta
     2701 tttacagaga tgtatcttat ctttccaacc tacatttgaa cgggtacatt tcgtgtcaag
     2761 aattacgtgc cggattccct cataattttc ctaattatgt catggggcaa atgctctgga
     2821 agaagacaag aagttatgaa gaattgattg aagaatactt ttctgctttg tatggggaaa
     2881 attggcagtc tgttgttgaa tatttagaaa aattatccat ttattcctct tgtgattatt
     2941 ttaatgcaat tggcagtcgt caaaatgatg ttttagcgaa tcattattat atagcttaca
     3001 atctagctga taatttttta ccaattattg aggaaaatat ttctaagtta ttaaatagtc
     3061 aaaaggatga atggaaacag ctcagttatc atcgtgaata tgttgttaag atggcgaagg
     3121 ctttatatct tcaagcaact ggaaaaacaa ggcaagctca agatgaatgg agaaatgtgt
     3181 tgaattatat ccgtgggcac gaattgctat ttcaatctaa tttggatgtt tatcgtgtaa
     3241 ttgaagtagc aaaaaattac gctggtttcc acttataaat cataagtata gaaaatgaac
     3301 taaggtattc agagaagatt gaccctaaat attatgaaat ttaaggattt ttaagatatt
     3361 tagggtcgac tttctatttg tatcgtagcg aagtcatttt aataatgatg tgtaaaagat
     3421 ggatcaagat tgaggaggaa gaaagatgaa atcaaaagaa gaaataaata tgcttggttt
     3481 tacaattgtc gcttacgcag gagatgcaag gtcagatttg atggatgctt tggcgtttgc
     3541 gagagatgga tattttgaac aggcaagaga attggttgag tctgcaaacg actcaatagt
     3601 gtctgcccat cgagaacaga ctaatttatt agcggaggag gcatatggag ataattttga
     3661 agtgagcttt attatgattc atggtcaaga tactttgatg acaacgatgc tattgtatga
     3721 tcaggtaaag ttttttattg atgaatatga acgaattcga aagattgaag aacatattgg
     3781 tttgcaatga ggattagtca tggaaaattt acagtttaaa gtcttaccga aggagttttt
     3841 attaggagct gctaccgctg cttatcaagt agagggtgca actagggtag atggcaaagg
     3901 aataaatatg tgggatgttt atttgcaaga aaatagtccg ttcttaccag atccagctag
     3961 tgatttttat tatcgttacg aagaggatat agctttggcg gcagaacatg gtttgcaggc
     4021 tttgcgttta tctatttctt gggttcgtat atttcctgat atagatggag atgctaatgt
     4081 attagctgtt cattattacc atagagtttt tcagtcttgc ttaaaacata atgtgattcc
     4141 gtttgtttct ttacatcatt ttgattcgcc tcagaaaatg ttagaaacag gggattggtt
     4201 gaatagagag aatattgatc gtttcatacg atatgctcgc ttttgtttcc aagaatttac
     4261 agaagtcaag cattggttta caatcaatga actgatgtct cttgctgcag gtcaatatat
     4321 aggaggtcag tttcctccaa atcatcattt tcaattatct gaagcaattc aagcgaatca
     4381 taatatgttg ttggcgcatg ctcttgcagt cctcgaattt catcaattag ggattgaggg
     4441 aaaggtaggt tgtattcatg ctttaaagct aggctatcct attgatgggc aaaaagaaaa
     4501 tattttggca gctaaacg
//
LOCUS       DAVGJL010000132         4495 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.133, whole genome shotgun sequence.
ACCESSION   DAVGJL010000132 DAVGJL010000000
VERSION     DAVGJL010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4495
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.133"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(138..896)
                     /locus_tag="VVE42_001843"
     CDS             complement(138..896)
                     /locus_tag="VVE42_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU3073989.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGFSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAVDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKVLTGEMTLMKLSGMIYDEGWLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR"
     gene            1114..1284
                     /locus_tag="VVE42_001844"
     CDS             1114..1284
                     /locus_tag="VVE42_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3073990.1"
                     /translation="MEIKEQTRKLAVSYSKYSFEVADKTDEVSNHTYGKATLTWFEEI
                     FEEYKEHHNIDV"
     gene            complement(1407..2537)
                     /gene="ugpC"
                     /locus_tag="VVE42_001845"
     CDS             complement(1407..2537)
                     /gene="ugpC"
                     /locus_tag="VVE42_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEU3073991.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFINVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
     gene            complement(2820..2905)
                     /locus_tag="VVE42_001846"
     tRNA            complement(2820..2905)
                     /locus_tag="VVE42_001846"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2869..2871),aa:Leu,seq:aag)
     gene            complement(2987..3562)
                     /locus_tag="VVE42_001847"
     CDS             complement(2987..3562)
                     /locus_tag="VVE42_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEU3073992.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDCFHPESKLFPPHNLIGTSGRNLYGDLGIFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELSE"
     gene            complement(3562..4350)
                     /gene="codY"
                     /locus_tag="VVE42_001848"
     CDS             complement(3562..4350)
                     /gene="codY"
                     /locus_tag="VVE42_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEU3073993.1"
                     /translation="MAHLLEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PVESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGKLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
BASE COUNT         1363 a          955 c          732 g         1445 t
ORIGIN      
        1 gccttccttt ggataatttt caattgttgc aatgtaatct tttaaattca tctttttctt
       61 tctttcaaag ttttttactc tctattatag catatttttt aagaaagaaa aaaggaaaag
      121 ttaacttcaa taattatcta acgttttgac gatttataac tagccatcgc aataaagccc
      181 aatttctgtt tattcttagc aaacatttta tacatagtta aaaactgctt tctattctcc
      241 tttttacaag catttacaca aattttcaaa gttcctagcc aaccttcgtc ataaatcata
      301 cccgataatt tcattaatgt catttcacca gtcaatactt tcacatcaca ataacctgat
      361 tctatcatca cctgttccca accatcttga gttaaaggac ctacatttac atgaattgct
      421 tgtgataatt cctgtctgat agactcttta gcttccttaa gaagcacatc atgtgtcaag
      481 agaagacctc caggttttaa tacccttaga tattccatta cacatttttt cttagcttga
      541 tcggcttgca tagtcagcat agcttcattt ataacaatat caaaactagc atcttgataa
      601 ggaagtttca ttgcatttgc tctttcaaaa ctgattaaat gagcaacacc tgccgttcca
      661 gcagattttt tagccacttc taaagcttga gcatccatat caacagcagt tatcttgcaa
      721 ccaaaacgct gtgccaactc aattgctgta gttcccctat tacacgcaac ctctagtatt
      781 ctcttttctt ttgaaaatcc tccttctgca attaaccaat ctgtggcacg ctttccacct
      841 ggacgtaagc gttttttccc caattttgct aaaaacttat gacctgcttc tgacatttga
      901 acacctctgt tttttcttca ttataacaca aaatgacaca aaatgatatt atctgacaat
      961 tctaatctat tatttgataa actagaatca aaagcataca agtcaccata actatagcat
     1021 aaatttatta ttacctctct agttcgaatt caattgctca cgtagtttac gcatatagct
     1081 tcacttgtag atggtaccca caaataatac tcaatggaaa tcaaagagca aactaggaaa
     1141 ctagccgtaa gctactcaaa gtacagcttt gaggttgcag ataaaactga cgaagtcagt
     1201 aatcatacgt acggcaaggc gacgttgacg tggtttgaag agattttcga agagtataaa
     1261 gagcatcaca atatagatgt ataatatgac ttccctagca aaaaggagag aagcattttg
     1321 tttctctccc tagattaact gatatctctc cactacaatt gataaagaga tatcttttct
     1381 tgtagtgctt tgaattttat ttattcttag tagattgttt tttcagtttc tacatcgaag
     1441 aagtgtgctt tgttcaagtc aaatccaagc tcaactgttg cacctgtttg caagtagtca
     1501 cgagcatcaa cttttgcaac aaactcgtct ttaccaactt ggcagtaaag gtgagattct
     1561 gaaccaagca gttctgatac agagatagtc gcttttacaa cacagtctgg gaatgtttca
     1621 aggaaagcag gttctgcatt cacgtcttct ggacggatac caaagatcaa ttcttttcct
     1681 tcgtagcctt tttcacgaag aactttcaat gctccttctg gcactttcaa acggaaaccg
     1741 tcagaaacaa tttcgctacc aaccaatttc acgttgatga agttcatagc tgggcttccg
     1801 atgaatcctg caacgaattt gttaactgga tttttgtaaa cttcttgagg agtaccgatt
     1861 tgttctacac gtccgatagt acctgtacca gcagggttct tagtagctga cataataacg
     1921 atacggtctg caagtgtcat cgcttctgtt tggtcgtgag ttacatagat agttgtagct
     1981 ccgatacgac ggtgaatttt agcgatttca gcacgcattg atacacgaag tttggcatcc
     2041 aagtttgaca aaggttcgtc catcaagaat actttcgcat cacggacaat cgcacgcccc
     2101 atggcaacac gttgacgttg accacctgaa aggtcagccg gtttacgttc caagaattct
     2161 ttcaatccaa gtatttcagc tgcttcttga acacgtttgt taatgtcttc tttgctgtat
     2221 ttacgcaatt tcaaaccgaa agccatgttg tcataaacag tcatgtgtgg gtaaagagcg
     2281 tagttttgga ataccatggc gatatcacgg tcttttggag ctacgtcgtt gacaaccacg
     2341 ccatcgatag atgcagtacc ttctgtgatg tcttcaagac cagcaatcat acggagtgta
     2401 gttgatttac cacatcctga aggtcctacg aaaacgataa attctttatc tttgatgttc
     2461 aagttgaaat cttcaactga atagtgttcg ctgtttggat attttttgta aatattttta
     2521 agattcaatt ctaccatgag gtgaactcct tttgtctttt gataattata ttatagatga
     2581 aaacgcttta tatttctatg gcaagatgac caaaaaaata aaaaagttct gtgcaacttg
     2641 cacaaaactt tagagaatat cggagaattt gaagaagata atctttatta ttcttctatg
     2701 acaaaagcag aaacaaggat cagttttccc atattttcgc tgattctcca ctacatttga
     2761 ctggattcta attttttaga gaaatacaaa agagctagct ttagctaact cttttcctat
     2821 gcggagagag ggacttgaac cctcacgacc taaagcggtc acaggatcct tagtcctgcg
     2881 cgtctgccaa ttccgccatc cccgcatcga ttactttact agtatatcaa cttttgggat
     2941 gcttgtcaac actttttttc aaattttttc attttcacca accaggttac tcagaaagtt
     3001 catttagatt ttcatctact aacttagctc cgagtgtatt tttgaaatga cctagggcaa
     3061 attgatgatt ttcaggccag atggaagcaa cagctggttt aacaatctcg atgtcatatc
     3121 ctagattata ggcatctata gctgtatgta ggacacagat atccgtcaag acacctgtta
     3181 agataacggt agacactcta cgctctctca aacgaatatc taggtcagtc cctgaaaaag
     3241 ctgagtaatg gcgtttatcc atccaaaaga cacgactgtc tgaaccatgc tcttgataaa
     3301 agatccccaa atctccatat aaattccgtc cactcgtccc aatcagatta tgaggaggaa
     3361 ataacttact ttccggatgg aaacaatcgt tttcttcatg agcatcaata gtaaagaaga
     3421 tataatctcc tcgttcaaaa gctaatcgag ttaccttgct gatggcatcc gaaatcgcct
     3481 gagctggagc acctgctgtc agcttcccac tatcagcaac aaaatcttct gtataatcaa
     3541 tcgaaattaa agcctttgtc attagtaatc tcttttcttc acttcttcaa aaatatctga
     3601 aatcaagacc ttaagatagg ttcccttcat tccaagtgag cgactttcaa taatccccgc
     3661 agactcaagt ttacgaagag cattgacaat cacagagcga gtgattccga tacgatctgc
     3721 aatcactgac gcagtcaact tcccttcatt tccatttaat tcccctaaaa ttgctgaaac
     3781 agcacggagt tcggagtaag aaagggtatt gaccgccatg gtgacagcag tacgacgacg
     3841 aatatttttc tcatcttctt cacgttggaa gttaagaagc tgaatcccaa caacggtact
     3901 ggcaatctca acaagaacca agtcctcatc ttcgaatttt ttatcattac gccaaataat
     3961 caaagaacca aggcgaatcc ccgatacatg aatcggtgca atagtcgtca agccatctgg
     4021 aaaatcatct ctactctcaa cagggaaaat actcatatca tgctcaacag gcaagtttgc
     4081 ttctgtttcg taaatcatat tagccccttg aacgtagtca tctgggaaaa tcttagtttg
     4141 gaagaattgc tctacgcgat ctgtatttgt tttataacgc ataaaatagc caagcagacg
     4201 tcccttacta ttgataatgc aggcattgca atgaataata tccgctaact gacgcgtaat
     4261 agcgttgtaa gggagctcat cctgcaactg ctcctctgag cgtttcaaaa ttgatgtaat
     4321 ttttctagtt ttttctaata aatgtgccat ttttcacctc gaatttaatc gctatcatta
     4381 taacataaaa acgtctcttt ttcaataatt atctgaaaat tccttattga cttgcattga
     4441 cttacaattt aattaaaaac cagaatattt ttaattaaat tgttcctttt ctatt
//
LOCUS       DAVGJL010000133         4305 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.134, whole genome shotgun sequence.
ACCESSION   DAVGJL010000133 DAVGJL010000000
VERSION     DAVGJL010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4305
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.134"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(42..1457)
                     /locus_tag="VVE42_001849"
     CDS             complement(42..1457)
                     /locus_tag="VVE42_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEU3073994.1"
                     /translation="MTIFPDDFLWGGAVAANQVEGAYNEDGKGLSVQDVLPKGGLGEA
                     TENPTEDNLKLIGIDFYHKYKEDISLFSEMGFNVFRTSIAWSRIFPKGDEEEPNEAGL
                     KYYDELFDELHAHGIEPLVTLSHYETPLYLARKYHGWIDRRMIHFYEKFARTVLERYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDIPKENLSKQELYQAIHHELVASSLVTKIAREI
                     NSEFKVGCMVLAMPAYPMTPNPKDVWATHEYENLNYLFSDVHVRGYYPNYAKRYFKEH
                     DINIEFAAEDAELLKNYTVDFLSFSYYMSVTQSALPTQYNSGEGNIIGGLVNPYLESS
                     EWGWQIDPIGLRIILNRYYDRYQIPLFIVENGLGAKDQLIKDELNNLTVQDDYRIQYM
                     KEHLLQVAEALQDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGNGTFNRY
                     KKMSFTWYKGVIESNGESLFK"
     gene            complement(1470..3308)
                     /locus_tag="VVE42_001850"
     CDS             complement(1470..3308)
                     /locus_tag="VVE42_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEU3073995.1"
                     /translation="MSYKDTVQKILDVIGGEKNVNRVTHCVTRLRLELKDENLVNDDD
                     VKKIPDVIGIMKKNGQYQIILGNDVANYYKEFVKLGNFESDSVVQGHKGNILERIIEY
                     IAGSMTPIIPAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDAPYYFLPLLLAY
                     SASQKLKVTSTLAMSVAGVLLHPNFVQMVQSGNPLSLFGAPVTPASYGSSVVPILIMV
                     WLMKYIEKIIAKLTPAITKSFLQPTLVLLVSSCIALVVVGPIGVIVGEGLSNLVGQMY
                     GVAGWLTLAILGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAAS
                     MAVALKSKNNNTKQIAFAAGFSALLAGITEPALYGVTLKYKKPLYAAMIGGGLAGLFA
                     GLTSVKAYLFAVPSLIALPQFIYSDVPSNIVNALIVAVISVVITFVLAYIFGIDEEES
                     SSNLEVEVGVSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFD
                     GKITNIFPTKHAIGLKSDEGVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEM
                     DLNLIKTKGYETVTPVIVTNTNDFLDVLVLPNNQTIEHSKELLVIL"
     gene            complement(3326..4165)
                     /locus_tag="VVE42_001851"
     CDS             complement(3326..4165)
                     /locus_tag="VVE42_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEU3073996.1"
                     /translation="MIIKRILNHNAVIAQSKKDIDILLFGRGIAFGRKIGDKVNPIDI
                     EKSFFLKNRDNMTRFTEMFINVPLELVYITEKIINLGKITLGNNFDEIIYINLTDHIS
                     SSIERYKEGIIISNPLRWEISKYYKEEFELGKRALQIIKKELGIELPIDEAAFIALHF
                     VNANLENNFQESYKITEIIMGIEKIIQDFYCTEFNQDSIDYYRFITHIKLFAHRLVEN
                     TTYCDDDDEDLLALMKNKYPREYECGEQVAMFIQTEYNYLLTSSELVYLMAHIRRLTK
                     NLD"
BASE COUNT         1434 a          845 c          569 g         1457 t
ORIGIN      
        1 gttgccttaa ccgtgataat atctaatcat caataaacaa actatttgaa taaggattct
       61 ccatttgatt caatcactcc tttataccaa gtaaaggaca ttttcttata tcgattgaat
      121 gtgccatttc catcatcatt tcgatcaaca taaatgagac cgtacctttt agaaagttgt
      181 gcagtggaca tagaaacaca gtcaatacat ccccaagacg tatagcccat aatttccaca
      241 ccatcttgta gagcttcagc tacttgcaat aaatgttctt tcatatactg aattctataa
      301 tcatcttgga cggttaagtt attaagttca tcttttatta gttgatcttt agcacctaat
      361 ccattttcta ctataaataa tgggatttga taacggtcat aatatctatt taaaattata
      421 cgtagtccaa ttggatcaat ttgccatccc cactcagaag actctaaata aggatttact
      481 aagccaccaa taatattccc ttctcctgaa ttatactgtg ttggaagagc agattgagtc
      541 acactcatgt aatagctaaa ggataaaaaa tctacagtat aattttttaa taactctgca
      601 tcttcagctg caaactctat gttaatgtca tgttccttaa aatatctttt tgcataattc
      661 ggataataac ctctaacatg cacatctgaa aatagataat ttagattctc atactcatga
      721 gtcgcccata catcttttgg atttggagtc attggataag ctggcatagc taataccata
      781 caccccacct taaactctga attaatctca cgagcaattt ttgtaaccaa actcgaggcg
      841 actaattcat gatgtatagc ttgatataat tcttgtttcg aaagattctc cttaggtata
      901 tctattcctc cactagtaaa cggtaattcc aaaacagagt ttacttcgtt aaatgtaagc
      961 caatatttaa ctttatcttt atacctttct aaaactgttc gagcaaattt ttcataaaaa
     1021 tgaatcattc tcctatcaat ccatccatga tattttcttg ctaaatataa tggagtctca
     1081 tagtgtgaaa gagttacaag tggttctatc ccgtgagcat gtagttcatc aaacaattca
     1141 tcataatatt tcaacccagc ttcgttaggt tcttcctcat ctccttttgg aaaaattcta
     1201 ctccatgcaa tagaagtacg aaaaacatta aagcccattt cagaaaacaa ggatatatct
     1261 tccttatatt tatgataaaa atcaatacct atcaatttta agttatcttc tgtaggattt
     1321 tctgttgctt ctcctaatcc acctttgggt aacacatcct gaactgataa gcccttacca
     1381 tcttcattat atgctccctc tacttgatta gctgcaacag ctccacccca aagaaaatca
     1441 tctggaaaaa tggtcataac tttcctccat tataatatta ccagtaattc cttagaatgc
     1501 tcgattgtct gattattagg taatactaat acatctagaa aatcattggt attcgttaca
     1561 attactggtg taactgtttc gtagccttta gtcttgatta aattcaagtc catttcaaaa
     1621 atcaactgat ttttgaaaac tctgtctcct tcttctacat gactaataaa accttgacct
     1681 tttagctcaa cagtatctaa tccaatatga attagtaact caacaccctc atcactcttc
     1741 aatccaattg cgtgcttagt cggaaaaata tttgtaattt tcccatcaaa tggtgcataa
     1801 accttacctt cacttgggat aatcgctact ccgtctccaa ttagtttatc tgaaaatgtt
     1861 ttatcctgga catcgcttaa cggaatgatt tctcctgata taggagaaaa tatcattttt
     1921 ttatttgaaa ctccaacttc aacttctaaa ttgctagaac tctcttcttc atcgattcca
     1981 aatatataag ctaatacaaa ggtaataaca accgaaatga ccgccacaat taaagcattt
     2041 acaatatttg atggcacatc agaataaata aattgaggca acgctatcaa agatgggaca
     2101 gcaaatagat atgctttaac actagtaaga cctgcaaata atcccgctaa tccaccacca
     2161 atcatagctg cataaagcgg ttttttatat tttaaagtca caccatataa tgcaggttcg
     2221 gtaatccctg caagtaaggc tgagaaacct gctgcaaaag caatttgttt tgtattatta
     2281 tttttactct ttaatgcaac agccatcgaa gcagcccctt gagctaagtt tgaccctaac
     2341 attgctggaa gaattaatac gtctggagta gcaatagatg ccgccaaaaa aataggtgca
     2401 aaagcccaat gcattccagt cataacaata aatggcataa tagcaccaag aatagctaat
     2461 gtaagccatc cagctacacc atacatttgc ccaactagat ttgataatcc ttcaccaaca
     2521 attactccaa taggtccgac tacaactaag gcaatacagc ttgatactaa taatactagc
     2581 gtaggttgca aaaaactctt agtaatagct ggtgttaatt tagcaattat tttttcaata
     2641 tatttcatca accaaaccat aataagaatt ggaacgactg atgaaccata actagctggt
     2701 gtcacaggtg caccaaataa actaagagga ttccctgatt gcaccatttg aacaaaattt
     2761 ggatggagaa gtacacctgc tacagacata gctaatgtag atgttacttt taatttttgt
     2821 gatgcagaat aagctaataa cagcggtaag aaataatatg gagcatcccc aaaaaatgtc
     2881 aaaaaagcaa tagtctgaga atctgattgc aatataccaa gcattggtaa aatgattacc
     2941 aagactttca acatacctcc ccctaacatt gctggaatga ttggagtcat ggaaccagcg
     3001 atatactcaa tgattctttc taaaatattc cctttgtgcc cttgaacaac tgaatcggat
     3061 tcaaaattgc caagtttaac gaattcttta taataattag ctacatcatt accaagtata
     3121 atttgatatt gtccattctt tttcataata cctattacat ctggtatctt cttcacatca
     3181 tcatcattga ctaaattttc atcttttaat tctaatctta aacgtgttac acaatgggta
     3241 actctattga catttttttc acctccaatt acatcgagga ttttttgtac cgtatcttta
     3301 taactcatgg tattctccta ttctattaat ctaaattttt tgttaagcga cgaatatgag
     3361 ccatcaaata aactaattca ctagaagtca gcaaataatt gtactccgtt tgtataaaca
     3421 ttgccacctg ttcaccacat tcatattctc taggatattt atttttcatt aatgctaaca
     3481 agtcttcatc atcatcgtca caataagttg tattttcaac caagcgatgg gcaaataatt
     3541 ttatatgagt tatgaatcta taataatcaa tagaatcttg gttaaactca gtacaataga
     3601 aatcttgaat gattttctca attcccataa ttatttcagt gattttatac gactcttgaa
     3661 aattattttc taaattagca ttaacaaaat gtagcgctat gaatgcagct tcgtcaattg
     3721 gaagttcaat acctaactct ttttttatta tttgtaaagc ccttttccca agttcaaatt
     3781 cttctttata atatttcgat atttcccagc gtaggggatt cgaaataata atcccttctt
     3841 tataacgttc tatgctcgaa gaaatatgat ccgttaaatt aatatagata atttcatcaa
     3901 aattattacc caatgttatt ttacctaggt taattatttt ttcggtgatg tacaccaact
     3961 ccaaaggaac gttaataaac atctctgtaa aacgggtcat attatctcta tttttgagaa
     4021 aaaaactttt ctcaatatca attggattta ctttatctcc aatttttctt ccaaaagcta
     4081 ttccccttcc aaaaagaaga atatcgatat cttttttact ttgcgcaatt acggcattgt
     4141 ggtttagtat tcgttttatg atcataaaat ttccctttta aaataaaagg aacctactta
     4201 aaaaacatca agaaacttct tctgatgtga ttaaataggt tcctgctcac ttgtattagt
     4261 gataacatcc ttttatttca atttttaatg gtgacatgaa gctta
//
LOCUS       DAVGJL010000134         4274 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.135, whole genome shotgun sequence.
ACCESSION   DAVGJL010000134 DAVGJL010000000
VERSION     DAVGJL010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4274
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.135"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            720..1712
                     /locus_tag="VVE42_001852"
     CDS             720..1712
                     /locus_tag="VVE42_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU3073997.1"
                     /translation="MAMIEVEHLQKHFVKIVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQDNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPNSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQEIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYEGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
     gene            1714..2532
                     /locus_tag="VVE42_001853"
     CDS             1714..2532
                     /locus_tag="VVE42_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEU3073998.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVLYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            2534..3319
                     /locus_tag="VVE42_001854"
     CDS             2534..3319
                     /locus_tag="VVE42_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU3073999.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLAQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISMVFFVISLKLWDKGLDSYESAGS"
     gene            3417..3635
                     /locus_tag="VVE42_001855"
     CDS             3417..3635
                     /locus_tag="VVE42_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HEU3074000.1"
                     /translation="MKDLLEWFGIPESTQAYIEGTTTLQVWTAYQESDLTGFVRLSYS
                     SEDCAEIDCLGVKKLIKVEKLGANCLLL"
     gene            3620..3817
                     /locus_tag="VVE42_001856"
     CDS             3620..3817
                     /locus_tag="VVE42_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU3074001.1"
                     /translation="MLATLESEARKKVGYLQVKTVAEGSNKDYDRTNDFYRGLGFKKL
                     EIFSQLWNPQNPCQILIKKLE"
     gene            complement(4135..>4274)
                     /locus_tag="VVE42_001857"
     CDS             complement(4135..>4274)
                     /locus_tag="VVE42_001857"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3074002.1"
                     /translation="MKIKEQTRKLAAGCSKHCFEVVDRTDEVSNHTHGKATLTWFEEI
                     F"
BASE COUNT         1186 a          736 c          922 g         1430 t
ORIGIN      
        1 cgaatcttca tcagaatgat aaaattaatc aattgatatc tgattacaaa cagaatatga
       61 aagcttttta tatcactatt gaaaaattta tacgaaatga tgaaagcctt aagatgatga
      121 aagccttaag tgttatttta caaaggttat ttcaagtcgt tccaaggtaa caagtctaga
      181 tcagattgaa gctgataaaa cgatacaaag aaaatattca agtgagctaa aaaaatttat
      241 tggattttat aatgagatta tttgtgagga aaatagtttc ctacatgtac gaaagaggtg
      301 gtcgagttga tttaggtagt cggtgcgtga gttgataatt ctcagggtat ggacttcttt
      361 ttcatgaatg aggtaaaaga gcaggtattg tttagagaca atcattctga gcatattttc
      421 tggatagagg gagtatccga ttttatgatc aaagttaata ccgccctctg gtgagaagat
      481 gagtaggttg gtaatttaaa ctattaaaca gaatttttga ttaaaagtat tattttatga
      541 gagaaatcct aattttacaa tccataggca aacgcttgca tttcgttttt tattggacta
      601 taataggttg gtataaagcc ttttgtagta ataaaatgta gaaggtgtag aaagtaagga
      661 tttagaatat ttgtagttaa aaacacaatg ttgctattcc ttacgatagg gagatagata
      721 tggcaatgat agaagtggaa catcttcaga aacattttgt gaagattgtt aaggaaccgg
      781 gcttgaaggg ggctttgcgc tcctttattc atcctgaaaa gcagaccttt gaagcggtca
      841 aggatttgac ctttgaggtt ccaaaagggc agattttagg atttatcggg gcaaatggtg
      901 ctgggaagtc gacaaccatt aaaatgctga caggaatttt gaaaccaaca tctggttttt
      961 gtcggattaa cggcaagatt ccccaggaca atcggcaaga ttatgtcaaa gatattggcg
     1021 tagtctttgg acaacgcacc cagctatggt gggatttggc tctgcaagag acctacactg
     1081 tcttaaaaga gatttatgat gtgccaaact cgctctttca taagcgcatg gactttttga
     1141 atgaagtctt ggatttgaag gactttatca aggatcccgt gcggactctt tcactgggac
     1201 aacggatgcg ggcggatatt gcggcctcct tgctccacaa tcccaaggtt ctttttttag
     1261 atgagccgac cattggtttg gacgtttcgg ttaaggataa tattcgtcgg gcaattactc
     1321 agattaatca agaggaagaa actaccattc ttttgaccac tcacgatttg agtgatattg
     1381 agcaactttg tgatcggatt ttcatgattg acaaggggca agagattttt gatggaacgg
     1441 tgagccaact caaggagacc tttggtaaga tgaagactct ctcttttgaa ctgctaccag
     1501 gtcaaagtca tctcgtctct cactatgaag gtctgtctga tatgaccatt gatagacaag
     1561 gaaacagcct caacattgaa tttgatagtt ctcgctacca gtcagctgac attatcaagc
     1621 aaaccctgtc tgattttgaa atccgcgatt tgaagatggt ggatacggat attgaggata
     1681 ttatccgtcg cttctaccga aaggagctct aggatgatca aattgtggag acgttataaa
     1741 ccctttatca atgcaggggt tcaggagttg attacttacc gagtcaactt tattctctat
     1801 cggattggcg atgtcatggg ggcttttgtg gccttttatc tctggaaggc tgtctttgat
     1861 tcttcgcaag agtctttgat tcagggcttc agtatggcgg atatcaccct ctacatcatc
     1921 atgagttttg tgaccaatct tctgactaga tccgattcgt cctttatgat tggggaggag
     1981 gtcaaggatg gctccattat catgcgtttg ttgcgaccag tgcattttgc ggcctcctat
     2041 cttttcaccg agcttggttc caagtggttg atttttatca gcgttggcct tccgttttta
     2101 agtgtcattg tcttgatgaa aatcatatcg ggtcaaggta ttgtagaggt gctaggatta
     2161 actgtccttt atctttttag cttaacgctc gcctatctga ttaacttttt ctttaatatt
     2221 tgctttggat tttcagcctt tgtgtttaaa aatctttggg gttccaacct acttaagact
     2281 tccatagtgg cttttatgtc ggggagtttg attcccttgg cattctttcc aaaggttgtt
     2341 tcagatattc tttccttttt gcctttttca tccttgattt atactccagt tatgatcatt
     2401 gttggaaaat acgatgccag tcagattctt caggcactcc ttttgcagtt cttctggctc
     2461 ttagtgatgg tgggattgtc tcagttgatt tggaaacggg tccagtcctt tatcaccatt
     2521 caaggaggtt agtatgaaaa aatatcaacg aatgcatctg atttttatca gacaatacat
     2581 caaacaaatc atggaatata aggtggattt tgtggttggt gtcttgggag tctttctggc
     2641 tcaaggcttg aatctcttgt ttctcaatgt catctttcaa catattccat tcctagaagg
     2701 ctggaccttt caagagatag ctttcattta tggattttcc ttgattccca agggaatgga
     2761 ccatctcttt tttgacaatc tctgggcact agggcaacgc ctagtccgaa aaggagagtt
     2821 tgacaagtat ctgactcgtc ccatcaatcc tctctttcac atcctagttg aaacctttca
     2881 gattgatgcc ttgggtgaac tcttagtcgg tggtatttta ttgggaacaa cagtgaccag
     2941 cattgtttgg actcttccaa aattcctgct tttcctagtt tgtattcctt ttgcgacctt
     3001 gatttatact tctcttaaaa tcgcaacagc cagtatcgcc ttttggacta agcagtcagg
     3061 cgccatgatt tacatcttct atatgttcaa tgactttgct aagtatccga tttctattta
     3121 caattctctt cttcgttggt tgattagctt tatcgtgcct ttcgccttta cagcctacta
     3181 tccagctagc tatttcttac aggaaaagga tgtattcttt aacgtaggag gtttgatgtt
     3241 gatttctatg gttttctttg ttatttccct taaactttgg gataagggct tagattccta
     3301 cgaaagtgcg ggttcgtaaa agctaaagta agactaaaat caagaaagga acttatgatg
     3361 tttgtaattg aagaagtcaa ggatgaaaat caaaaaaggc agttgtcgct gagattttga
     3421 aggatttgct agaatggttt ggaatcccag aaagcacaca agcctatata gaaggaacca
     3481 cgacactgca agtttggacc gcctatcagg agagtgattt gactggattt gtaaggttat
     3541 cctattcgag tgaagattgt gcagagattg attgtctcgg cgtaaaaaag cttatcaagg
     3601 tagaaaaatt gggagccaat tgcttgctac tttagagagt gaagctcgta aaaaagttgg
     3661 ttatctgcag gtcaaaacag tggcagaagg ttctaataaa gattatgatc gaacaaatga
     3721 cttttatcga ggtcttggct ttaaaaagtt agagattttt tctcagctat ggaatccgca
     3781 aaatccttgt cagattttga ttaaaaagct tgaataatat tacttgacat ctattctcag
     3841 agtgccatac tgtaagtgta atcgccgatt tagctcagtt ggtagagcaa ggcactcgta
     3901 aagcctaggt tataggtaga taaacgactg aggagttgaa aaaatagata ggtagaagat
     3961 aaccgttaag ccttattctt agcggttatt tatattgttt aatagcgctg atatttcatc
     4021 aattatgcct gttttcgtgt ttctggtagt tgttcaagtt tattgctact atttttgatg
     4081 gtatgaatgt gcttataatg tatctcggtt aacgaaagtt ttgaacttat actcttagaa
     4141 aatctcttca aaccacgtca acgtcgcctt gccgtgcgta tggttactga cttcgtcagt
     4201 tctatccaca acctcaaaac agtgttttga gcaacctgcg gctagtttcc tagtttgctc
     4261 tttgattttc attg
//
LOCUS       DAVGJL010000135         4182 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.136, whole genome shotgun sequence.
ACCESSION   DAVGJL010000135 DAVGJL010000000
VERSION     DAVGJL010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.136"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..1168
                     /locus_tag="VVE42_001858"
     CDS             <1..1168
                     /locus_tag="VVE42_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU3074003.1"
                     /translation="IDNVKVDDKVCEIISEIMDESYTDIQILTHKLYSKGDDIKASKT
                     IWMKKSGKEYSEAFAGTGEARIILLVNDIVNAQSNSLILIDEPEISLHPSAIYKFKEF
                     LLQECLNKKHQIIITTHSTQLIKDFPREAVKLLVKNGEKVDVIENIDYQDAFFELGDV
                     YHSRKMIYVEDRLAKYILEFVITHSGSENLKQNLVVRYIPGGANQIICNNILNSSYLD
                     SDNHYFWLDGDQNTNVSESNNLMNYLENGVVISDKIPESDNKNLDDIIKLITGCPIKF
                     NVSGNKGQKNNIELIAKQRSFIDYWAKYVSYLPFPTPEFFLANLCNSVDREGYDFSKD
                     GNGKEYFRKKTQVALGIENITSEDIFQEQRRAVSKIQPESSMFQCIKEKLEALF"
     gene            1548..2351
                     /locus_tag="VVE42_001859"
     CDS             1548..2351
                     /locus_tag="VVE42_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HEU3074004.1"
                     /translation="MQPTYNIDNPNLSYEAKRDLWRIGFGLQKVDNLVPSAYMESLAE
                     KQSRGELTYEQVYEDATAYHHTIDASTEEADLVSLRIVELLSRRGFSFSPAILLAIHK
                     ELFQDIFEPSIPVGQFRQTNITKNEPVLNGESVVYSDYSMIQMTLDYNFNQEKQVAYA
                     TLTQADVVKQIQHFISGIWQIHPFREGNTRTVTVFLIQYLREFGFDIDNIPFQQHSKY
                     FRDALVLDNAKILQRRPEFLTAFFENLLLGGQNDLSSEKMYLDLDLDLS"
     gene            complement(2467..3816)
                     /locus_tag="VVE42_001860"
                     /pseudo
     CDS             complement(2467..3816)
                     /locus_tag="VVE42_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1332 a          674 c          737 g         1439 t
ORIGIN      
        1 tatagataat gttaaagttg atgataaagt ttgtgaaatt attagtgaaa taatggatga
       61 atcttataca gacattcaaa ttttaacaca taagttgtat tcaaaaggtg atgatataaa
      121 agcttccaaa actatttgga tgaaaaaatc tggaaaagaa tattccgagg cttttgcagg
      181 aacaggtgag gctagaatta ttttgttagt aaatgatata gtaaatgcgc aatcaaattc
      241 attaatatta atagatgaac ctgaaatctc acttcatccg agtgcaatct ataaatttaa
      301 agagttttta cttcaagagt gtttaaataa aaaacatcaa attattatca ctacacattc
      361 tacacaactt ataaaagatt ttcctagaga agccgtgaaa cttttagtga aaaacggaga
      421 aaaggtagat gttattgaaa atattgatta tcaggatgca ttttttgaat taggtgatgt
      481 gtatcattct aggaagatga tttatgttga agatagacta gctaaatata ttctagagtt
      541 tgttatcact cattcaggta gtgagaatct taaacagaat ttagtagtga gatatattcc
      601 tggtggagca aatcaaataa tttgtaataa tattttaaac tcatcgtatt tagattccga
      661 taaccattat ttttggcttg atggagatca aaacactaat gttagtgaat caaataattt
      721 aatgaactat cttgaaaatg gtgttgttat atcagataaa attcctgaat cagataataa
      781 aaatcttgat gatattataa aattgataac gggatgtcca attaaattta atgtttcagg
      841 taataaaggg caaaaaaata atattgaatt aattgcgaaa caaagaagct ttatagatta
      901 ttgggctaaa tacgtttctt atcttccttt cccaacccca gagttttttt tagctaattt
      961 gtgtaacagc gtagataggg aaggttatga tttttctaaa gatggcaacg gaaaagaata
     1021 ttttagaaaa aaaactcaag tagcgttagg aatagagaat ataacttctg aagatatttt
     1081 ccaggagcaa agaagagctg tttcaaaaat acagccagaa tcatcaatgt ttcagtgtat
     1141 taaagaaaaa ttagaggcat tgttctaatt ccttagatta ggtactacat aacaaatttt
     1201 ttaaattcaa acccgcacca aaaacaacat tgcaactttc ttctcaggta atgtgttggg
     1261 atttgaacag accaacgagt tcatagtggt gtatgccaat gactttgata agtctacgcg
     1321 tcagacttat tagttcattt atccagatat atctcgataa tcatattatt tagtcaaaga
     1381 acatcccata tgagggcatg aaatgctaag tttagcttgc tcactattaa gatgaagtat
     1441 atatatttca tagaacctat tgtgaaggat ttttataatt ttcgccaatt ccatacctat
     1501 ttcacaattt accgatgaca cagaaaaaca attgaaagga tatttctatg caaccaactt
     1561 acaacattga caatccaaac ttgtcttatg aagcgaagcg tgatttatgg cggataggtt
     1621 ttggtctgca gaaagttgac aatctagtgc catcagcgta tatggaatct ttggctgaga
     1681 aacagtcccg gggtgaactg acttatgagc aggtttatga ggatgcaacg gcttatcatc
     1741 ataccattga tgcgagtaca gaggaggcag acttggtttc tctacgtatt gtagaactat
     1801 tgtctcgaag aggctttagc ttcagtcctg cgatcttact tgctattcat aaggagttgt
     1861 ttcaagatat atttgaaccc tctattccgg tggggcaatt tcgtcagacc aatatcacaa
     1921 agaatgaacc tgttttgaat ggtgaaagtg ttgtgtactc tgattactcc atgattcaaa
     1981 tgaccttgga ttataatttt aatcaggaaa aacaagttgc atatgcgaca ctaactcagg
     2041 cggatgtggt taaacaaatc cagcatttta tttcaggaat ctggcagatt catccatttc
     2101 gcgaaggaaa cactcggaca gtaacggtct ttttgattca gtatcttcgt gagtttggtt
     2161 ttgatattga taatatacca tttcagcaac attccaaata ttttcgtgat gccttggtat
     2221 tagataatgc aaagatttta cagcgacgtc ctgagttttt aacagctttt tttgaaaatc
     2281 tcttgctcgg tggtcaaaat gatttgtctt cagaaaaaat gtatctagat ttagacctcg
     2341 atctttcata atcctaatac tgagtaaaca ttgaatttta ggaaaaaatg aagtaaatat
     2401 tctcacaata aaacgcataa tagtgggtgt agaatgaact gccccccaaa aagttagaca
     2461 attaatttat ccgaaggatt tagttctgta ttgcacaggg ataagtcctt ttagttttac
     2521 cttaattcgt ttattgttgt agtaatcaat atagtctaca atagcttgtt ccaattgatt
     2581 aagtgattta aatgttttct catagccata aaacatttcc gatttcagaa tcccaaagaa
     2641 ggactccatc ataccgttgt ctgggctgtt acccttacgt gacatggatg cttgaattcc
     2701 cttaccctca agaaactgat gataagaatc gtgttgatat tgctagcctt ggtcactatg
     2761 gagaatcgta ttctcgtagt gcttctctgt gaatgcctgt tccaacattg tttgtacttg
     2821 ttctaagttg ggtgaagttg aaagattata ggcgataatt tcgctgttaa agccatctaa
     2881 aactggtgat aagtaaagct tttgagtact tgctggaatg gcaaattcag tcacatctgt
     2941 gtagcactgt tccattgttt tagagccttc aaattggcct tgaatgagat tctctgcctt
     3001 cttgccaacg tctcctttat gagaagaata ttttcgtttc tgtcgcattt tagcttgtaa
     3061 attgagtact ttcatcaagc cttgaactct tttatgattt accagataac cacgatttct
     3121 tagttctaaa tgaacccggc gataagcata atttcccttg tgtttgataa aaatggattg
     3181 aatttcagct ttaagctctt ggtccttatc tggtttatct agctgtttca agtgatagta
     3241 gtaggtccaa cgagctagtt taatggcttt tagaagaaga tctaacgaaa actcagtcat
     3301 taattcttga ataattttct gtctttcttc tttctctttt tcctccttca atcggagttc
     3361 tcttaacttt tttaggatgg cattctccgc tctcaggtac tcattttctg attgaagacg
     3421 ttctaattct gtcctctctt caggtctcgt ttttggatta cgtcccattt taggtactct
     3481 ccctcttgtt ttctcaacaa tagtataccc gtttttcctg tattgtgcta gccaaaatga
     3541 aagcattcct tggtttggga gggcataatc aagactaaca cttctttgtg aacaaccttc
     3601 cagtaaggct ttatccatca tttcttgttt caatttagaa gaatagtgac gattcttccc
     3661 ctttttaacg atttctatgc cataacggtc aattaatttc accatgtact ttagaccaga
     3721 aacatccaca ccaaatcttt ttgaaagctg tttgaagctt tgtccttgct ttcttagttc
     3781 atagatctga actttatctt cataactcaa tttcataata aaacacccca aaagttagat
     3841 ttattctgtc taacttttgg ggtgcagttc acagttgaag agccagaaaa ttaaagtgtc
     3901 ttttactcaa tcaaaaatag taacgcctat tcttttggtg aataagacta ttgtttggta
     3961 tggtgcaatg ccattattag ggaaggtagc tgagatgacc atattacgtt tggaatcagc
     4021 tagtatagtt tctgaactag tggcaggttt acgatagaga aaatttttaa aaatttctat
     4081 gtatgatttt catttcttta gtgagactgt tgccattatc acattcgaat cacacaaaat
     4141 aaaaaaattt ttataagtac ttgacaaata gattgaaata tc
//
LOCUS       DAVGJL010000136         4027 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.137, whole genome shotgun sequence.
ACCESSION   DAVGJL010000136 DAVGJL010000000
VERSION     DAVGJL010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.137"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            10..963
                     /locus_tag="VVE42_001861"
     CDS             10..963
                     /locus_tag="VVE42_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU3074005.1"
                     /translation="MKNKRIFKDFQASKMSLNIYTSPLLAFVFVFIGEFVAFTLYGIG
                     LLALIGLARNFGEAGQNLATYLQTLHQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENFLSNLLKGFSLGLALFLLTLLGLVVLGQYRLESIHLNPYSLAF
                     VVFTIPFWILQGTAEEVVARAWLLPQLASRTNLKLAILISSLFFTLLHMGNSGLTPLS
                     LVNLFLFGVAMALYLLKTDTVWGVAGIHGAWNFAQGNLFGILVSGQPSGTSLMTFLPQ
                     GNQDWLSGGSFGIEGSIMTSLVLLLLIVYLANKLKKENERM"
     gene            1040..3028
                     /gene="uvrB"
                     /locus_tag="VVE42_001862"
     CDS             1040..3028
                     /gene="uvrB"
                     /locus_tag="VVE42_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEU3074006.1"
                     /translation="MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAVAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTMGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARVEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMAYNEEHGIVPQTIKKEIRDLIAVTKAVAKEEDKEVDINSLNKQE
                     RKELVKKLEKQMQEAVEVLDFELAAQIRDMMLEVKALD"
     gene            3038..3601
                     /locus_tag="VVE42_001863"
     CDS             3038..3601
                     /locus_tag="VVE42_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU3074007.1"
                     /translation="MIYLRKLKKEDLMSLWEMAYSQLNPVWKQYDAPYYDDYQYFSNF
                     KEFELQKSESILSNSNRLGIFVDDKLVGTVSRYWVCKETRWMELGIGIYDKKFWNTGI
                     GKVAMLQWIDRTFQDYLELEHLVLTTWSGNLGMMKLAEKLRMKKEAHIPKVRYYQGKY
                     FDSIKYGILREDWEKINDGYYQINGNS"
     gene            3567..>4027
                     /locus_tag="VVE42_001864"
     CDS             3567..>4027
                     /locus_tag="VVE42_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU3074008.1"
                     /translation="MTVIIKSMETPEEIEGKSLVHWQTWRESYDDLLPAEFQDTMTLE
                     RCRFFSQKYPENTLIAMDGVKVVGFISYGNFRDETIQAGEIIALYVLKDYYGKGIAQK
                     LMKAALTALNHFSEIFLWVLKDNKRAIAFYQKMGFTFDGQEKILELGKPIK"
BASE COUNT         1170 a          710 c          934 g         1213 t
ORIGIN      
        1 gacggggata tgaagaacaa aagaatattt aaagacttcc aagcttcaaa aatgagttta
       61 aacatttaca caagcccctt gttagccttt gtttttgtct tcataggaga gtttgtggct
      121 tttactttgt atggtattgg cttgttagct ctcatcggac ttgctagaaa ttttggagag
      181 gctggtcaaa atcttgcaac ctacttgcag accttgcatc agagcttgac ggataaaaca
      241 agtgactttc gtttaatttt aggattactg gcctttggtt ttattcttaa cactgtgttc
      301 agatggacaa gaaaagttga gaaaagacct attcgaacct tgggatttta tagagagaat
      361 ttcctcagca atcttctgaa aggatttagt ctaggcttgg cactttttct tctgaccttg
      421 ttaggtttag tggtcttagg tcaatatcgt ttggaatcca ttcacttgaa tccttattct
      481 cttgcctttg tcgtctttac tatcccattt tggattttac aggggacagc agaagaagtg
      541 gtggcccgtg cttggctact tcctcaattg gcctcaagaa ccaatctaaa actagctatt
      601 cttatatcta gcctgttctt taccctgctt catatgggca attctggcct cacccctcta
      661 tctctagtaa atctcttttt attcggagtt gccatggctc tttatcttct caaaactgat
      721 acagtttggg gtgttgcagg tattcatggt gcttggaatt ttgctcaggg taatctcttt
      781 gggattttag ttagtggtca accgtcagga acgtctctga tgaccttttt accacaaggc
      841 aatcaagatt ggctatcagg tggttctttt ggcatagaag gttccattat gacaagtctg
      901 gtattactac tgctgattgt ctatcttgct aataaattaa agaaagaaaa tgaaaggatg
      961 tgacttcggt ccgtcctttt cttcgtgaaa atactataag tatgctaaaa taggaatagc
     1021 acatggagag aggattctta tgatcaatca cattacagat aatcaattta aactagtatc
     1081 aaaatatcaa ccatcaggag atcaacccca agctatcgag cagttggtgg ataacattga
     1141 ggggggagaa aaagctcaga ttctgatggg ggcgactgga acagggaaga cctatactat
     1201 gagtcaggtc atttctaaag tcaataaacc aactctggtt attgcccaca ataaaactct
     1261 ggctggtcag ctctatgggg agtttaagga atttttccct gaaaatgcag ttgagtattt
     1321 cgtatcctac tatgattatt accagccaga ggcctatgtc ccttctagcg atacctatat
     1381 tgagaaggat agttctgtca atgacgagat tgacaagctt cgccactcag ctacctcagc
     1441 ccttttggag cgtaatgatg ttattgtcgt ggcctcagtc tcttgtatct atggtttggg
     1501 ttcgcccaag gaatacgctg atagtgtcgt tagtctccgt cctggtctag agatttctcg
     1561 tgataaactc ttgaatgact tggtcgatat tcagtttgaa cgtaatgata ttgatttcca
     1621 acgcggaaga tttcgcgttc gtggggatgt ggtagagatt ttcccagctt cccgagatga
     1681 acatgccttt cgagtagaat tttttggaga cgaaattgac cgtatccgtg aggttgaggc
     1741 tctgacaggt caggtattgg gagaggtgga tcatttggcg attttcccag ctactcactt
     1801 tgtgaccaat gacgaccata tggaagttgc cgttgccaag attcaagcag aattagaaga
     1861 gcagttagct gtctttgaga aggaaggtaa attgcttgaa gcccagcgct tgaaacaacg
     1921 gacagagtac gatatcgaaa tgctgcgcga gatgggctat acaaatgggg ttgaaaatta
     1981 ttctcgccac atggatggac gtagtgaagg tgagcctcct tatacgcttc tcgacttttt
     2041 cccagatgat tttttgatta tgattgacga gagtcatatg actatgggac aaataaaagg
     2101 catgtacaat ggagaccgtt cgcgtaagga aatgctagta aattatggtt tccgtttacc
     2161 gtctgccttg gacaatcgtc ctctccgtcg tgaggagttt gaaagccatg tgcatcagat
     2221 tgtttacgta tcagcgacac ctggtgacta tgaaaatgaa cagactgaga cggtgattga
     2281 gcaaatcatt cgaccgacag ggcttttgga tcctgaggtg gaagtccgtc cgactatggg
     2341 acagattgat gatctcttag gtgaaatcaa tgcccgtgtt gaaaagaatg aacgaacctt
     2401 tatcaccacc ttgaccaaga aaatggcaga ggacttgacc gactatttca aggaaatggg
     2461 catcaaggtc aagtacatgc actcggatat caagaccttg gaacggacgg agattatccg
     2521 tgatctccgc ttgggtgtct ttgatgtctt ggttggaatc aatctccttc gtgaagggat
     2581 tgacgttcct gaggttagct tggttgccat tctcgatgct gacaaggaag gtttccttcg
     2641 taacgagcgt ggactcatcc agaccattgg gcgtgccgcc cgtaacagtg aaggtcatgt
     2701 catcatgtat gcagatacgg ttacccagtc tatgcaacgt gctatcgatg aaactgcccg
     2761 ccgtcggaaa attcagatgg cctataatga agagcatggt atcgtaccac agacaatcaa
     2821 gaaagaaatc cgtgacctga ttgccgtgac caaggcagtt gctaaggaag aggacaagga
     2881 agtcgacatc aatagcctca acaaacaaga gcgcaaagaa ctcgtcaaga aactagaaaa
     2941 acaaatgcaa gaagccgtcg aagtgcttga ctttgaactg gcggctcaga tccgtgatat
     3001 gatgttggaa gtcaaggcct tggattaggg gaatagtatg atttatttaa gaaagttaaa
     3061 gaaagaagat ttgatgtctt tatgggaaat ggcttattca cagcttaatc cagtttggaa
     3121 acagtatgat gctccctatt atgatgatta tcagtatttt tcaaatttta aagaattcga
     3181 actacaaaaa tcagaatcca ttttaagcaa ctcaaatcgc cttggtattt ttgttgatga
     3241 taaactagtt gggactgttt cgcgttattg ggtatgtaaa gaaacaagat ggatggaatt
     3301 gggaattggt atttatgata aaaaattctg gaacactggt attgggaaag ttgctatgtt
     3361 gcagtggata gataggacgt ttcaggatta cttggagttg gagcatctgg ttttgacaac
     3421 ttggtcagga aatcttggta tgatgaaact tgctgaaaaa ttaagaatga aaaaagaagc
     3481 tcatattcca aaagttcgtt attatcaagg taaatatttt gatagtatta aatatggtat
     3541 tttgagagaa gactgggaga aaataaatga cggttattat caaatcaatg gaaactcctg
     3601 aagagataga aggcaaatcc ctcgttcact ggcaaacgtg gagagagtct tatgatgacc
     3661 ttttgcctgc ggaatttcag gatacaatga cattagaaag atgtcgattc tttagtcaaa
     3721 agtatccaga aaatacattg attgcgatgg atggcgtgaa ggtggttggt tttatcagtt
     3781 atggcaattt tcgtgatgag actattcaag ctggtgaaat tattgcttta tatgttttaa
     3841 aagactatta tggaaaaggt atcgcacaaa agttaatgaa agcagctttg actgctctta
     3901 atcatttttc tgaaattttc ttatgggtat tgaaagataa caagcgcgcc attgctttct
     3961 atcaaaaaat gggttttact tttgatggac aagaaaaaat acttgaactt ggaaagccta
     4021 taaagga
//
LOCUS       DAVGJL010000137         3681 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.138, whole genome shotgun sequence.
ACCESSION   DAVGJL010000137 DAVGJL010000000
VERSION     DAVGJL010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.138"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            153..380
                     /locus_tag="VVE42_001865"
     CDS             153..380
                     /locus_tag="VVE42_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3074009.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
     gene            668..3550
                     /gene="nanA"
                     /locus_tag="VVE42_001866"
     CDS             668..3550
                     /gene="nanA"
                     /locus_tag="VVE42_001866"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored neuraminidase NanA"
                     /protein_id="HEU3074010.1"
                     /translation="MNRSIQERKCRYSIRKLSVGAVSMIVGAVVFGTSPVLAQEGASE
                     QPLANETQLSGGSSTLTDTEKSQPSSETELSGNKQEQERKDKQEEKIPRDYYARDLEN
                     VETVIEKEDVETNASNGQRVDLSSELDKLKKLENATVHMEFKPDAKAPAFYNLFSVSS
                     ATKKDEYFTMAVYNNTATLEGRGSDGKQFYNNYNDAPLKVKPGQWNSVTFTVEKPTAE
                     LPKGRVRLYVNGVLSRTSLRSGNFIKDMPDVTHVQIGATKRANNTVWGSNLQIRNLTV
                     YNRALTPEEVQKRSQLFKRSDLEKKLPEGAALTEKTDIFESGRNGNPNKDGIKSYRIP
                     ALLKTDKGTLIAGADERRLHSSDWGDIGMVIRRSEDNGKTWGDRVTITNLRDNPKASD
                     PSIGSPVNIDMVLVQDPETKRIFSIYDMFPEGKGIFGMSSQKEEAYKKIDGKTYQILY
                     REGEKGAYTIRENGTVYTPDGKATDYRVVVDPVKPAYSDKGDLYKGNQLLGNIYFTTN
                     KTSPFRIAKDSYLWMSYSDDDGKTWSAPQDITPMVKADWMKFLGVGPGTGIVLRNGPH
                     KGRILIPVYTTNNVSHLNGSQSSRVIYSDDHGKTWHAGEAVNDNRQVDGQKIHSSTMN
                     NKRAQNTESTVVQLNNGDVKLFMRGLTGDLQVATSKDGGVTWEKDIKRYPQVKDVYVQ
                     MSAIHTMHNGKEYIILSNAGGPNRENGMVHLARVEENGELTWLKHNPIQKGEFAYNSL
                     QELGNGEYGILYEHTEKGQNAYTLSFRKFNWEFLSKDLISPTEAKVKRTREMGKGVIG
                     LEFDSEVLVNKAPTLQLANGKTATFLTQYDSKTLLFAVDKEDIGQEIIGIAKGSIESM
                     HNLPVNLAGARVPGGVNGSKAAVHEVPEFTGGVNGTEPAVHEIAEYKGSDSLVTLTTK
                     EDYTYKAPLAQQALPETGNKESDLLASLGLTAFFLGLFTLGKKREQ"
BASE COUNT         1260 a          623 c          830 g          968 t
ORIGIN      
        1 aaaaatgagg tataataaat ttactaacgc ttataaaaag tgatagaatc tatctttatg
       61 tatatttaaa gatagattgc tgtaaaaata gtagtagcta tgcgaaataa cagatagaga
      121 gaagggattg aagcttagaa aaggggaata atatgatatt taaggcattc aagacaaaaa
      181 agcagagaaa aagacaagtt gaactacttt tgacagtttt tttcgacagt tttctgattg
      241 atttatttct tcacttattt gggattgtcc cctttaagct ggataagatt ctgattgtga
      301 gcttgattat atttcccatt atttctacaa gtatttatgc ttatgaaaag ctatttgaaa
      361 aagtgttcga taaggattga gcaggaagta tggtataaat agcataagct gatgtccatc
      421 atttgcttat aaatagatat tttagtttaa ttgcagcggt gccctggtag ataaactaga
      481 ttggcaggag tctgattgga gaaaggagag gggaaaattg gcaccaattt gagatagttt
      541 gtttagttca tttttgtcat ttaaatgaac tgtagtaaaa gaaagttaat aaaagacaaa
      601 ctaagtgcat tttctggagt aaatgtctta tttcagaaat cgggatatag atatagagag
      661 gatcagtatg aatcggagta ttcaagaacg taagtgtcgt tatagcatta ggaaactatc
      721 ggtaggagcg gtttctatga ttgtaggagc agtggtattt ggaacgtctc ctgttttagc
      781 tcaagaaggg gcaagtgagc aacctctggc aaatgaaact caactttcgg gggggagctc
      841 aaccctaact gatacagaaa agagccagcc ttcttcagag actgaacttt ctggcaataa
      901 gcaagaacaa gaaaggaaag ataagcaaga agaaaaaatt ccaagagatt actatgcacg
      961 agatttggaa aatgtcgaaa cagtgataga aaaagaagat gttgaaacca atgcttcaaa
     1021 tggtcagaga gttgatttat caagtgaact agataaacta aagaaacttg aaaacgcaac
     1081 agttcacatg gagtttaagc cagatgccaa ggccccagca ttctataatc tcttttctgt
     1141 gtcaagtgct actaaaaaag atgagtactt cactatggca gtttacaata atactgctac
     1201 tctagagggg cgtggttcgg atgggaaaca gttttacaat aattacaacg atgcaccctt
     1261 aaaagttaaa ccaggtcagt ggaattctgt gactttcaca gttgaaaaac cgacagcaga
     1321 actacctaaa ggccgagtgc gcctctacgt aaacggggta ttatctcgaa caagtctgag
     1381 atctggcaat ttcattaaag atatgccaga tgtaacgcat gtgcaaatcg gagcaaccaa
     1441 gcgtgccaac aatacggttt ggggatcaaa tctacagatt cggaatctca ctgtgtataa
     1501 tcgtgcttta acaccagaag aggtacaaaa acgtagtcaa ctttttaaac gctcagattt
     1561 agaaaaaaaa ctacctgaag gagcggcttt aacagagaaa acggacatat tcgaaagcgg
     1621 gcgtaacggt aacccaaata aagatggaat caagagttat cgtattccag cacttctcaa
     1681 gacagataaa ggaactttga tcgcaggtgc agatgaacgc cgtctccatt cgagtgactg
     1741 gggtgatatc ggtatggtca tcagacgtag tgaagataat ggaaagacat ggggtgaccg
     1801 agtaaccatt accaacttac gtgacaatcc aaaagcttct gacccatcga tcggttcacc
     1861 agtgaatatc gatatggtgt tggttcaaga tcctgaaacc aaacgaatct tttctatcta
     1921 tgacatgttc ccagaaggga agggaatctt tggaatgtct tcacaaaaag aagaagccta
     1981 caaaaaaatc gatggaaaaa cctatcaaat cctctatcgt gaaggagaaa agggagctta
     2041 taccattcga gaaaatggta ctgtctatac accagatggt aaggcgacag actatcgcgt
     2101 tgttgtagat cctgttaaac cagcctatag cgacaagggt gatctataca agggtaacca
     2161 attactaggc aatatctact tcacaacaaa caaaacttct ccatttagaa ttgccaagga
     2221 tagctatcta tggatgtcct acagtgatga cgacgggaag acatggtcag cgcctcaaga
     2281 tattactccg atggtcaaag ccgattggat gaaattcttg ggtgtaggtc ctggaacagg
     2341 aattgtactt cggaatgggc ctcacaaggg acggattttg ataccggttt atacgactaa
     2401 taatgtatct cacttaaatg gctcgcaatc ttctcgtgtc atctattcag atgatcatgg
     2461 aaaaacttgg catgctggag aagcggtcaa cgataaccgt caggtagacg gtcaaaagat
     2521 ccactcttct acgatgaaca ataaacgtgc gcaaaataca gaatcaacgg tggtacaact
     2581 aaacaatgga gatgttaaac tctttatgcg tggtttgact ggagatcttc aggttgctac
     2641 aagtaaagac ggaggagtga cttgggagaa ggatatcaaa cgttatccac aggttaaaga
     2701 tgtctatgtt caaatgtctg ctatccatac gatgcataat ggaaaagaat acatcatcct
     2761 cagcaatgca ggtggaccga atcgtgaaaa tgggatggtc cacttggcac gtgtcgaaga
     2821 aaatggtgag ttgacttggc tcaaacacaa tccaattcaa aaaggagagt ttgcctataa
     2881 ttcgctccaa gaattaggaa atggggagta tggcatcttg tatgaacata ctgaaaaagg
     2941 acaaaatgcc tataccctat catttagaaa atttaattgg gaatttttga gcaaagatct
     3001 gatttctcct accgaagcga aagtgaagcg aactagagag atgggcaaag gagttattgg
     3061 cttggagttt gactcagaag tattggtcaa caaggctcca acccttcaat tggcaaatgg
     3121 taaaacagcg actttcctaa cccagtatga tagcaagacc ttgttgtttg cagtagataa
     3181 ggaagatatc ggacaggaaa ttattggtat agctaaagga agcatcgaaa gtatgcataa
     3241 tcttcctgta aatctagcag gtgccagagt tcctggcgga gtaaatggta gcaaagcagc
     3301 ggtgcatgaa gttccagaat ttacaggggg agttaatggt acagagccag ctgttcatga
     3361 aatcgcagag tataagggat ctgattcgct tgtaactctt actacaaaag aagattatac
     3421 ttacaaagct cctcttgctc agcaggcact tcctgaaaca ggaaacaagg agagtgacct
     3481 cctagcttca ctaggactaa cagctttctt ccttggtctg tttacgctag ggaaaaagag
     3541 agaacaataa gagaagaatt ctaaacattt gattttgtaa aaatagaagg agatagcagg
     3601 ttttcaagcc tgctatcttt ttttgatgac attcaggctg atacgaaatc ataagaggtc
     3661 tgaaactact ttcagagtag t
//
LOCUS       DAVGJL010000138         3654 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.139, whole genome shotgun sequence.
ACCESSION   DAVGJL010000138 DAVGJL010000000
VERSION     DAVGJL010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3654
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.139"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(141..1046)
                     /locus_tag="VVE42_001867"
     CDS             complement(141..1046)
                     /locus_tag="VVE42_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001192128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU3074011.1"
                     /translation="MQSVAPLVTYLFVVAISILDGFAAPVSYAIVPRYATDLGKANSA
                     LSMTGEAVQLIGWGLGGLLFATIGLLPTTFIILVLYIISSFLMLFLPNAEVEVLESET
                     NLEILLKGWKLVARNPRLRLFVSANLLEIFSNTIWVSSIILVFVTELLNKTESYWGYS
                     NTAYSIGIIISGLIAFRLSEKFLAAKWESILFPLVAMAIVTLTILYFPNAQMFLLFSA
                     LVGMLSQLKEVPESVFLQETVEENHLVNVYSVLEVISTLAFSVFVLLMSYITESFGIS
                     ISFWLSAICLMIEAILIYIRRDYFK"
     gene            complement(1046..1318)
                     /locus_tag="VVE42_001868"
     CDS             complement(1046..1318)
                     /locus_tag="VVE42_001868"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3074012.1"
                     /translation="MSNSFVKLLVSQLFANLADIFFRVTIIANIYIISKSVIATSLVP
                     ILIGISSFVASLLVPLVTKRLALNRVLSLSQFGKTILLAILVGCLP"
     gene            complement(1774..3051)
                     /gene="ltrA"
                     /locus_tag="VVE42_001869"
                     /pseudo
     CDS             complement(1774..3051)
                     /gene="ltrA"
                     /locus_tag="VVE42_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         1168 a          769 c          592 g         1125 t
ORIGIN      
        1 aatagagtga tcctgcttca aaactccatt tcaggagaca atgaagtaaa tcttcccata
       61 ataaaacaca caatatcaag ttttttcaac acctgatact atgcgctttt ctgattttta
      121 aagacttttt aaccactctc tcatttaaaa taatctcgtc tgatataaat taaaatagct
      181 tctatcatca gacaaatggc tgatagccaa aaactgatgc taataccaaa actctcagta
      241 atatagctca ttagcaaaac aaatactgaa aatgctaatg tagaaatcac ttcaagaacg
      301 gaatagacat taactaaatg attttcctct actgtttcct gaagaaatac actttcagga
      361 acttctttta gttgcgataa cataccaact aaagctgaaa ataataaaaa catctgtgcg
      421 tttggaaaat atagaatagt cagtgtcact attgccatag ctacaagagg aaaaagaata
      481 ctttcccatt tagcagcaag gaacttttca gatagcctaa aagcaattaa gccactaatt
      541 ataataccaa tagagtatgc tgtattagaa tatccccagt aactttccgt tttatttaat
      601 aactccgtta caaaaacaag tataatggaa gaaacccaaa tcgtatttga aaaaatttcc
      661 aataaatttg ctgatacaaa aagtcttaat ctaggatttc tagcaactaa cttccaacct
      721 ttgagcaaaa tttcaagatt agtttctgac tctaacacct ccacttcagc gttaggaaga
      781 aataacatca gaaagctaga aatgatatac aagactaaaa tgataaacgt ggtaggtaac
      841 agaccaattg ttgcaaacaa gagtccacct aatccccaac ctatcaattg aacagcttca
      901 ccagtcattg ataaggctga attagcctta cccaaatcgg tcgcatagcg tggcacaata
      961 gcataggaaa cgggtgctgc aaaaccatct agtatggaaa ttgcaacaac aaatagatag
     1021 gtcaccaaag gcgctacgga ttgcattacg gtaaacatcc taccagtatc gccaataata
     1081 tagtctttcc aaattgagat aaagataaaa ccctatttag cgctaacctt ttagtaacca
     1141 acggaactaa aagactcgca acaaaagagg atattcctat taagatagga actagtgatg
     1201 tggcaattac tgattttgaa ataatgtata tgttagcaat gattgttact ctaaagaaaa
     1261 tatctgctaa atttgcaaat aattgagaga ctaacaactt gacaaatgaa ttagacatgt
     1321 agcgctcccc ctttagttac aaactttgga attaaatttg atttttaata ttttagcata
     1381 aaatagcaga aaacaaaagt gaagtttttt acatgaaaaa acaaaaacta ttgcctttat
     1441 tattcctaat tttaggagga attatgatta tcgttgttgg ctagtactaa gaagttagtt
     1501 agttagtaga ataattacat cttcaatcga aaaagagact ggaaaaagtc ttaaaaatca
     1561 gaaaaacgct caaatccttt ggcgctctaa gcttttctaa tgatagattt agatagtcaa
     1621 aaaaatatct gaaaagttcc caagatcgtc gtctcagcat caaaaatacg tgaaatttgc
     1681 ataattaaaa taaatttcag gggagttaat cgagtagggg ataatctcta gcccctctca
     1741 caccaccgta cgtgccgttc ggcatacggc ggttcaacta acttttaacg catgtcgttc
     1801 aaggtaataa tccaaacacg aaaccaatcc acgtttttcc aggactggtt ttgatatagc
     1861 acgtttaagt accgacttct gagctactaa ttgataatgg tcgccccagc cagatacctt
     1921 atctgctatc catttaggaa ctcctaactt aagcaatccc cataatcgtc tcgatttctt
     1981 cttccattgc ttccagataa tcactcgtag gcgagtacgc aagcgctcat ctatgctggc
     2041 gactatactt ttcatatttc ccaatgagaa atagtttatc catcctcgaa tagacaaatt
     2101 cagttgctca atacgtcttg ttaggtctat actccatttc ctctgtgtta gtttcttcaa
     2161 tttaaactta aatctccgaa cactatcttg atgtggacgg cttttccaac catctgatga
     2221 tttccagaac ccaaaaccta gatatttcaa ctctcttggt ctggtaatct tagtcttggt
     2281 catgtttact ttcaaaccta gccgtttctc aataaaacga ctgactgaat acatcacacg
     2341 cttagcggct gcctcgcttc cgaccgtaat cacacaatca tctgcgtagc gcacaaatcg
     2401 aagtcccctc ttttctaatt ccttgtccaa ttcattaaac atgatattgg ataagagagg
     2461 agataaattt cctccctgtg gtgtaccaac tagtgtttta taacgttgac cattaatgat
     2521 aacacctgaa tgaagatact tacgaatcaa ggattccgta tctccgtctt cgataatgtt
     2581 atgtactaag gacatcaatc tatcttgagg aactgtatcg aaaaatttct ctaggtctat
     2641 atccactatc cactcatagc cgtcatttaa gtattctaag agcttcatga tggctttttc
     2701 acatgaccta tttggtctga aaccataact cgtatctgag aaatggggtt cacaaatggg
     2761 gttcatgact tagacaatgg cctgttgaat cattctatcc ataactgttg gaattcctag
     2821 ttgacggatg cctccgtctg gtttaggtat ctcaactcta agaactggtt gaggattata
     2881 ttttctctgt tttatcagtt ccttagtcaa gcgccagttt tgtctgagat aattatccat
     2941 ctcttcgata gtcattccat caatcccagc tgagccttta ttggatttta cttgattgta
     3001 ggcttccagc atattttcgc gtgataatat cttatctagc aattttgaca tgtgcgtatt
     3061 cctttcttgt tttggtgcct gagggacatc ttatattggt gagccttcat ctagtcaata
     3121 cgtgacagac tccagttcta tcagacctct cgttttacag acacaagtga agtaggatta
     3181 cttcaattag ttgttcaccc cttcgctcca cttccattac agaagtttcc tcactactat
     3241 gggttcggct gacttctcat gattcgttgt tactacgctt tcagcgctca tgtgacctca
     3301 cgggataagt cgtacatctt tcctcgttta tacaagatat gaggacgtta aaaggttaca
     3361 gtgaaaatag gaaatctttt cacacaaacc ttagtccgaa ttcaattact ctcgtgattt
     3421 acacatatag gttacgacta cctttttgga ctttagagtt tgaagcctcc ttatccgcta
     3481 tatacgcctt actatcacgt tcctgttcgt agagccacga tttcgctatc ccttcctctc
     3541 cctgctacct cacgatagtc aggcttggga atcgctattg ggttcaccgg tagcaggtgc
     3601 ccaccgagga cttacacctc agatatacga catgcccgtc gtactacaaa aaaa
//
LOCUS       DAVGJL010000139         3627 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.140, whole genome shotgun sequence.
ACCESSION   DAVGJL010000139 DAVGJL010000000
VERSION     DAVGJL010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.140"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            33..701
                     /locus_tag="VVE42_001870"
     CDS             33..701
                     /locus_tag="VVE42_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU3074013.1"
                     /translation="MVEQYLPLYQKAFFLTLHIAVWGILGSFLLGLIVSIIRHYRILV
                     LAQVATAYIELSRNTPLLIQLFFLYFGLPRIGIVLSSEVCATLGLVFLGGSYMAESFR
                     SGLEAISQTQQEIGLAIGLTPLQVFYYVVLPQATAVALPSFSANVIFLIKETSVFSAV
                     ALADLMYVAKDLIGLYYETDIALAMLVVAYLIMLLPISLVFSWIERRLRHAGFGNPST
                     LSRK"
     gene            661..1338
                     /locus_tag="VVE42_001871"
     CDS             661..1338
                     /locus_tag="VVE42_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU3074014.1"
                     /translation="MQDSGIQVLFQGNNLLRILQGLGVTIEISILSVLLSMMFGTVMG
                     IIMTSHSRIIRFLTRLYLDFTRIMPQLVLLFIVYFGLARNFNINISGETSAIIVFTLW
                     GTAEMGDLVRGAITSLPKHQFESGQALGLTNVQLYYHIIIPQVLRRLLPQAINLVTRM
                     IKTTSLVVLIGVVEVTKVGQQIIDSNRLTIPTASFWIYGTILVLYFAVCYPISKLSTH
                     LEKHWRN"
     gene            1339..2097
                     /locus_tag="VVE42_001872"
     CDS             1339..2097
                     /locus_tag="VVE42_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU3074015.1"
                     /translation="MSETILEIKELKKSFGDNPILQGLSLEIKKGEVVVILGPSGCGK
                     STLLRCLNGLESIQGGDIFLDGQSIVENKKDFHLVRQKIGMVFQSYELFPHLDVLQNL
                     ILGPIKTQGRDKKEVTEEALQLLDRVGLLDKQHSFARQLSGGQKQRVAIVRALLMHPE
                     IILFDEVTASLDPEMVREVLELINDLAQEGRTMILVTHEMQFAQAIADRIIFLDQGKI
                     AEEGTAQAFFTNPQTKRAQEFLNVFDFSQFGSYL"
     gene            2109..2975
                     /locus_tag="VVE42_001873"
     CDS             2109..2975
                     /locus_tag="VVE42_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU3074016.1"
                     /translation="MKLFKPLLTVLVLAFALIFITACSSGGNAGSSSGKTTAKARTID
                     EIKKSGELRIAVFGDKKLFGYVDNDGSYQGYDIELGNQLAQDLGVKVKYISVDAANRA
                     EYLISNKVDIILANFTVTDERKKQVDFALPYMKVSLGVVSPKTGLITDVKQLEGKTLI
                     VTKGTTAETYFEKNHPEIKLQKYDQYSDSYQALLDGRGDAFSTDNTEVLAWALENKGF
                     EVGITSLGDPDTIAAAVQKGNQELLDFINKDIEKLGKENFFHKAYEKTLHPTYGDAAK
                     ADDLVVEGGKVD"
     gene            complement(3062..3613)
                     /locus_tag="VVE42_001874"
     CDS             complement(3062..3613)
                     /locus_tag="VVE42_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3074017.1"
                     /translation="MGITGANGSGKSVLFKLIAGLYSPSYGEVLINGENIVPERKIPA
                     NLGALIEEPGFINYYSGFKNLQYLASIRGVVGNQEINDTLKIVGLYEQKDQKVKTYSL
                     GMRKKLGIAQAIMENPSILLLDEPMNALDKSSVENMRTLFRELSSEKGTTILIASHSE
                     EDIRILCDKVYAIEDKVCTLCSD"
BASE COUNT         1041 a          811 c          674 g         1101 t
ORIGIN      
        1 ctagatggga ggttctattt ttggattgat ccattgttga acaatatcta ccactatatc
       61 aaaaggcatt ctttctgacc ttgcatattg cagtttgggg aattttggga tcctttctgc
      121 tcggtttaat cgttagtatc atccgacatt atcgaatcct tgttttggcg caagtagcga
      181 cagcctacat tgaattgtca cgtaatacgc cccttttgat tcaactcttc tttctctact
      241 tcggtcttcc ccgaatcggg attgtcctat cttcagaagt ctgtgcaacg cttgggcttg
      301 tctttttagg aggctcctat atggcagaat ctttccgaag tgggctggaa gccatcagtc
      361 aaacccagca ggagattggc ctcgctattg gtctgacacc tctacaggtc ttttactatg
      421 tggttcttcc gcaagcaaca gcggtggcac tcccctcctt tagtgccaat gtcattttcc
      481 ttatcaagga aacctctgtt ttctcagcag tggctttggc cgacctcatg tacgtcgcca
      541 aggatttgat tggtctctac tatgagacag acattgcgct agctatgttg gtagttgctt
      601 atctgatcat gctgctaccc atctcactgg tctttagctg gatagaaagg aggctccgcc
      661 atgcaggatt cgggaatcca agtactcttt caaggaaata atctcctgag aatcttacag
      721 ggattgggcg ttacgattga gatatccatc ctgtctgtcc tcttatccat gatgttcgga
      781 acagtcatgg gaatcatcat gacctcccat tctagaatca tacgattttt aacacgattg
      841 tatctggact ttacccgtat catgccccag ctggtgctac tcttcatcgt ttactttggc
      901 ttagctcgaa actttaatat caatatctca ggtgagactt cagctattat cgtttttacc
      961 ctctggggaa cagctgaaat gggagacttg gtacgtggag ctatcacttc tctccctaaa
     1021 catcagtttg aaagtggaca ggcactcggc ttgactaatg ttcaacttta ctaccacatc
     1081 atcatcccac aagtcttgag aagactgcta ccgcaggcta tcaatcttgt cactcggatg
     1141 attaaaacca cttcattagt tgttttgatt ggggttgtgg aagtgaccaa agttggacaa
     1201 caaatcatcg atagcaatcg cctgaccatc ccaactgctt cattttggat ttatggaacc
     1261 attctagtct tatatttcgc agtttgctac cctatttcca aactatccac tcacttagaa
     1321 aaacattgga gaaactaaat gtctgaaact atcttagaaa tcaaagaact aaaaaaatcc
     1381 ttcggagaca atcccatcct ccaaggactt tctctagaaa tcaaaaaagg ggaagttgtt
     1441 gtcatcctag ggccatctgg ttgtgggaaa agtaccctcc ttcgttgcct caacggctta
     1501 gaaagtattc aaggtggaga tatttttctg gatggtcagt ctatcgttga aaataaaaaa
     1561 gattttcacc tagttcgcca aaagattggc atggtctttc aaagttatga actctttccc
     1621 catctggatg tcttacaaaa cctcatccta ggccctatca aaactcaagg aagggacaag
     1681 aaagaagtaa cggaagaagc tttgcaatta ctagatcgtg tcggtttgct ggataaacaa
     1741 catagctttg cccgtcaatt atctggtgga cagaagcaac gtgttgcaat tgtccgtgcc
     1801 ctcctaatgc atccagaaat catccttttt gacgaggtga ctgcttcgct ggatccagaa
     1861 atggtgcgtg aggtgctgga acttatcaat gatttggccc aagaaggccg taccatgatt
     1921 ttagtaaccc acgaaatgca gtttgcccaa gccattgctg accggattat cttcctcgac
     1981 caagggaaaa tcgctgaaga aggaacagct caagccttct ttaccaatcc gcaaaccaaa
     2041 cgagcccagg aatttttaaa cgtctttgac tttagccaat tcggctcata tctataaagg
     2101 agattcttat gaaactattc aaaccactct taactgtttt agtacttgcc tttgccctta
     2161 tctttatcac tgcttgtagc tcaggtggaa acgctggttc atcctctgga aaaacaactg
     2221 ccaaagctcg cactatcgat gaaatcaaaa aaagcggtga actgcgaatc gccgtgtttg
     2281 gagataaaaa actgtttggc tacgttgaca atgatggttc ttaccaaggc tacgatattg
     2341 aactagggaa ccaactagct caagaccttg gtgtcaaggt taaatacatt tcagtcgatg
     2401 ctgccaaccg tgcggaatac ttgatttcaa acaaggtaga tattattctt gctaacttta
     2461 cagtaactga cgaacgtaag aaacaagttg attttgccct tccatatatg aaagtttctc
     2521 tgggtgtcgt atcacctaag actggtctca ttacagacgt caaacaactt gaaggtaaaa
     2581 ccttaattgt cacaaaagga acgactgctg agacttattt tgaaaagaat catccagaaa
     2641 tcaaactcca aaaatacgac caatacagtg actcttacca agctcttctt gacggacgtg
     2701 gagatgcctt ttcaactgat aatacggaag ttctagcttg ggcgcttgaa aataaaggat
     2761 ttgaagtagg aattacttcc ctcggtgatc ccgataccat tgcggcagca gttcaaaaag
     2821 gcaaccaaga attgctagac ttcatcaata aagatattga aaaattaggc aaggaaaact
     2881 tcttccacaa ggcctatgaa aaaacacttc acccaaccta cggtgacgct gctaaagcag
     2941 atgacctggt tgttgaaggt ggaaaagttg attagtcatt aactcttaaa aggaactgga
     3001 ttttaagctc caatcccttt ttaagatttt acctataaca tcctgagtct atctaagatg
     3061 ttcaatctga acacaatgta catactttat cttctattgc atatacttta tcacataaga
     3121 tacgaatatc ctcttcacta tgactagcaa tcaaaattgt tgtccctttt tcactagaga
     3181 gctctctaaa caatgttctc atattttcta cacttgattt atccaaggca ttcataggtt
     3241 catctagtaa aagaatagag ggattctcca taattgcttg agcaatccct agctttttcc
     3301 tcatacctag cgaataagtt ttaactttct ggtctttttg ctcatataga ccaactattt
     3361 tcagtgtatc attgatttcc tgattaccaa ctactcctcg tatgcttgcc aaatattgta
     3421 aattcttaaa gccactataa taatttataa aaccaggttc ttcaatcaaa gctcccaaat
     3481 tagctggaat ttttctctca ggaacaatat tttccccatt gattaacact tctccataag
     3541 acggactata taaaccagct attaatttaa acaatacact tttccctgag ccattcgcac
     3601 cagtaattcc tataatttcc ccctgtt
//
LOCUS       DAVGJL010000140         3580 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.141, whole genome shotgun sequence.
ACCESSION   DAVGJL010000140 DAVGJL010000000
VERSION     DAVGJL010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3580
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.141"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(89..1225)
                     /gene="lctO"
                     /locus_tag="VVE42_001875"
     CDS             complement(89..1225)
                     /gene="lctO"
                     /locus_tag="VVE42_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HEU3074018.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TLRENIRAFNHKLIVPHTLCDVENPSTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVA
                     TARGVHEFGSLYTTSSYSTVDLPEISEALQGTPHWFQFYFSKDDGINRHIMDRVKAEG
                     YKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSP
                     RDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQE
                     VAEAVDRRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEH
                     LNAELKTVMQLSGAQTIEDVKHFKLRHNPYNPTFPVDPRDLKLY"
     gene            complement(1850..3340)
                     /gene="lysS"
                     /locus_tag="VVE42_001876"
     CDS             complement(1850..3340)
                     /gene="lysS"
                     /locus_tag="VVE42_001876"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEU3074019.1"
                     /translation="MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQEL
                     KDKYANLDKEQLHDKNETATIAGRLITKRGKGKVGFAHLQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINEPFKRVHMVDAIREITGVD
                     FWQDMTLEEAKAIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            complement(3468..>3580)
                     /locus_tag="VVE42_001877"
     CDS             complement(3468..>3580)
                     /locus_tag="VVE42_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HEU3074020.1"
                     /translation="DFINTYDLEPMAQQVIPKAAFGYIASGAGDTFTSFQ"
BASE COUNT         1026 a          765 c          707 g         1082 t
ORIGIN      
        1 atttcatttt attatagttt tcgatagcga tttattcttc caatacacga agaaaaacct
       61 ccacattcag tggaggcaat ctgttttatc aatacaattt taagtcacga gggtcaactg
      121 ggaaggttgg gttgtatgga ttgtgacgga gcttaaagtg tttgacatct tcaatggtct
      181 gagctccaga caattgcata actgtcttca attccgcatt caagtgttca aagacttgac
      241 gcacaccgac actaccaccg agagccaagc cataaataac agggcgacca atagctacca
      301 agtctgctcc tgaagccaag gctttaaaga cgtgttgacc acgacgaaca ccagagtcaa
      361 agacaatcgg cacacgtcta tcaactgctt ctgccacttc ttgaagcgag tcaaaggcag
      421 ctggtccacc gtcgatttga cgaccaccgt ggttggttac ccagatacca gaagctcctg
      481 cagcaagtga acgttcaacg tcctcacggc attgtggtcc cttgacatac acaggaagac
      541 cagagtattc agcgataaat tctacatcgc gtggagacaa gcgttgttta gctgatttgt
      601 aaacaaagtc cattgattta ccagcacctt ctggcaggta ttcttcaaca atcggcatgc
      661 caactgggaa gacaaaacca ttacgcttat ccacttcacg attcccccct acagtagcat
      721 ctgccgtcaa gacaatcgct ttataacctt cagccttcac acggtccatg atgtggcggt
      781 tgataccgtc atccttacta aagtaaaatt ggaaccaatg gggtgtccct tgaagggctt
      841 cagaaatctc tggaaggtca acagtagagt aagagctggt tgtataaaga gaaccaaact
      901 catgcacacc acgcgcagtc gccacttccc cctgttcatt tgccaattta tgagccgcaa
      961 caggcgccat aatgattggt gaagatagtt tttcacctgc aaattcaatc tctgtacttg
     1021 gattttcaac atcgcaaagt gtatgaggaa cgatgagttt gtggttaaag gcgcggatat
     1081 tctctcttaa agtgaaagta tcttccgccc cactagcgat atagccaaat gctgctttag
     1141 gaataacttg ttgcgccatt ggctccaaat cataggtatt gatgaagtct acatgacctt
     1201 ctgcattgct tgttttgtat gacataaaat gccctccttg attaagtaag cgtttgcttt
     1261 ttactacaca actataataa ctcttttttc aatcattttc aagagttttg cttaaaaacc
     1321 atcttatttt ttcatacaat atactagccc tactttcctt aaactctaaa ctttagtcta
     1381 tattaagtga atcgttgaga ttttttgctg ccaggcattt gaactacttt tttatttgct
     1441 gattctatct actaatacta taaaaaaaca agtgaataag acaccagaac atcctctaat
     1501 aggacctatg gacttatatc aatcccatat atactgttta agcaagatac tatcctagtt
     1561 gacgctaact tcctatccca cttattaatt ttttatttat agtgaatctg aacaaatcaa
     1621 ttggggaatc caagtcaatt tctagtactt tttgaacagt ttcagcgtac tatcgtaaaa
     1681 tgaaataaaa catgcacaaa ttgcttaggg aatttaaacc aatttctaac aatgtcttag
     1741 aaatcaaagt gtactatttc aacttcaata tactacactt cattttctcc ttaaattcag
     1801 tctctttttg aatcaaaaaa tcctctggta agagccagag gataagaatt tatttcattg
     1861 ttgggaagag caatacatca cggatagttg ttgtatcagt gaggagcatg cagagacggt
     1921 cgataccgat tcccaaacca cctgttggtg gcataccgta ttcaagagct tcaatgtagt
     1981 catagtcgat tcctgtcgct tcatcatcac caagttcttt ggctttagct tgggcttcaa
     2041 aacggctaag ttggtcgatt gggtcgttga gttcagtaaa ggcattaccg tactccttag
     2101 tcatgataaa gagctcgaaa cggtcagtaa agcgttggtc ttcaggattt ttcttagcga
     2161 gtggagatac agctactgga tgtccataga caaaggttgg ttggattaaa gtttcttcaa
     2221 caaactcttc aaagaaggca ttgatgatgt gaccaacctc agtgtagtgt ttctcaactg
     2281 gaactttctt ctcagcagcg atagctttag cttcttctaa agtcatgtct tgccagaaat
     2341 cgacaccagt aatttctctg atagcatcca ccatatgaac acgcttaaat ggttcgttaa
     2401 tcttgatttc agtaccttgg tagttgactg ggccatcgcc cttaactgat ttagcagcgt
     2461 gttggataat gccttccgtc aagtccatga tgtcttggaa gtctgcataa gcttggtaaa
     2521 cttcgataga agtaaactca gggttatgag tagcgtccat tccttcgtta cggaagatac
     2581 ggccaatttc atagacacgt tccataccac ccacgataag gcgttttaag tgaagctcag
     2641 tcgcgatacg aagcaccatg tcaatgtttt gggcattgtg gtgggtgata aatggacggg
     2701 cagcagcacc accggcttca ttatgaagaa caggtgtttc cacttcaagg aatccttttt
     2761 ggtcaaggta acgacggatt tcagaaatga tttttgaacg agtgacaaag cgttcaaagc
     2821 tttcacggtt agaaatcaag tcaaggtaac gtttacggta aattgtttca acgtctgtca
     2881 aaccatggaa tttctcagga agaggacgaa gagccttaga caagtgtgtg atgtgggttg
     2941 ccttgataga gagttctccc atatccgtac gcatcacttc accttcgaca ccaaggaagt
     3001 caccaaggtc tgcttttttg aagatttcgt agttttcttc accgacagca tccttacgaa
     3061 cgtagatctg aatctggcct tcgcggtctt gaaggtgggc aaaaccaact tttcctttac
     3121 cacgtttggt tatcaagcgt cctgcgatag tagctgtttc gtttttatcg tgtaattgtt
     3181 ctttatcgag gttggcatat ttatctttta attcttgtga atttgcagta cgttcaaaac
     3241 gttttccgaa aggatcgatt ccttgttcgc ggagcgcagc cattttttca cggcgaacga
     3301 tctgctggtc atttagttct tccatatgtt ctgtagacat gggatcctcc tagttttact
     3361 caaaattgaa tacgatgagt cattttttgc gatactccca ttttatcata aataagcaag
     3421 aaattcacgg ataatcttta aaaactaaaa agaacctgac gctaaaatca ctggaaagaa
     3481 gtgaaagtat ctcccgcccc actagcgata tagccaaatg ctgctttagg aataacttgt
     3541 tgcgccattg gctccaaatc ataggtattg atgaaatcta
//
LOCUS       DAVGJL010000141         3553 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.142, whole genome shotgun sequence.
ACCESSION   DAVGJL010000141 DAVGJL010000000
VERSION     DAVGJL010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.142"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..1268
                     /locus_tag="VVE42_001878"
     CDS             <1..1268
                     /locus_tag="VVE42_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEU3074021.1"
                     /translation="KHLVNTQYKPEEHFSAEIGWINDPNGFVYFRGEYHLFYQFYPYD
                     SVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSGSAIVKDDRLWLMYTGHIE
                     EKTGVRQVQNMAFSDDGIHFEKISQNPVATGSDLPDELIAADFRDPKLFEKDGRYYSV
                     VAAKHKNNVGCIVLLGSDNLVEWQFESIFLKGVEHQGFMWECPDYFELDGKDCLIMSP
                     MRYQREGDSYHNINSSLLFTGKVDWGEKCFIPESVQEIDHGQDFYAPQTLLDDQNRRI
                     LIAWMQTWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQFPVKKGQYQIQIDKDCHY
                     HLGNDTDYLEFGYDSNVQQVYIDRSHLIQKILGEEEQDTSRRYVDIEAKELEVVLDKN
                     SIEIFVNQGEASLTATYYLTVPAELSRID"
     gene            complement(1478..1550)
                     /locus_tag="VVE42_001879"
     tRNA            complement(1478..1550)
                     /locus_tag="VVE42_001879"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1515..1517),aa:Lys,seq:ctt)
     gene            complement(1815..3086)
                     /locus_tag="VVE42_001880"
     CDS             complement(1815..3086)
                     /locus_tag="VVE42_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEU3074022.1"
                     /translation="MPDNLALRMRPKTIDQVIGQEHLVGHGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     ITLSNPERGFDFPIELDEDALDFIATSTNGDLRSAFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYAARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQKIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALADLKTSGHLPIPRHLRDGHYSGSKELENAQDYLYPHNYPGNWVKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDRLRKI"
     gene            3356..>3553
                     /locus_tag="VVE42_001881"
     CDS             3356..>3553
                     /locus_tag="VVE42_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3074023.1"
                     /translation="MKREVISNGNDGPSQEILIFTKQIRHWILSDQVISGKRKLFFRE
                     DTPKEILDLYENIKSKLDFAYQ"
BASE COUNT         1048 a          662 c          806 g         1037 t
ORIGIN      
        1 ataaacatct tgttaatact caatataagc ctgaagaaca tttttcagct gagattggtt
       61 ggatcaatga tccaaatgga tttgtctatt ttcgtggaga ataccatctc ttttatcaat
      121 tctatccata tgatagtgtt tgggggccta tgcactgggg acatgctaaa agtaaggact
      181 tggtgacttg ggagcacttg ccagtggcac ttgctcctga tcaagattat gaccgaaatg
      241 gttgtttctc aggctctgcc attgtcaagg atgatcgcct ctggctcatg tacactgggc
      301 atatcgaaga aaaaaccggt gtccgccaag tgcaaaatat ggcattttca gatgacggga
      361 ttcactttga aaagatttcc caaaatccag ttgcaactgg atcagacttg ccagatgagt
      421 tgattgctgc tgatttccgt gatccaaaac tctttgaaaa agatggacgc tattactccg
      481 tagtagctgc caaacacaag aataatgtgg gctgtatcgt tctactaggg tccgataacc
      541 tagtagaatg gcagttcgaa tccatctttt taaaaggggt agaacaccaa ggctttatgt
      601 gggaatgccc agattacttc gagttagatg ggaaagattg ccttattatg tcacccatgc
      661 gttatcagcg tgagggagac tcatatcata acatcaactc atcgcttttg ttcacgggta
      721 aggtagattg gggagaaaaa tgttttatcc cagaatcagt tcaagaaatt gatcatggcc
      781 aagacttcta tgcgcctcaa acattgttgg acgatcaaaa tcgtcgtatc ctgattgctt
      841 ggatgcagac atgggggcgt acccttccaa cccatgacca agaacacaag tgggcatgtg
      901 ccatgacttt acctagaatt ctaagattgg aagatggcaa actaagacaa ttccctgtta
      961 aaaaaggtca atatcaaatc caaatagata aagattgtca ttaccactta ggaaatgata
     1021 cagattatct tgaatttggt tatgacagta atgtgcagca agtttacatt gatcgtagcc
     1081 atcttattca aaaaattcta ggtgaagaag aacaggacac tagtcgacgg tatgtagata
     1141 ttgaagctaa agaattggaa gttgttctag ataaaaattc catcgagatt tttgttaatc
     1201 aaggtgaagc aagcttgact gcaacttatt acttaacggt gccagctgag ctatcacgaa
     1261 ttgattaaaa attaagttat ttctcctaaa gaaaaagttc tctttctaaa atagtggaaa
     1321 gaggactttt tgtgttttgg gtatataagc ttagtttatg gtatttgtaa aattggtgtt
     1381 ggattatgat ttaaactagt tttctaaaga atttgaaaaa aattttattt aagcaaaaaa
     1441 agccctatat tataagactt ttcctgttgt atttagatgc cccctacagg gatcgaacct
     1501 gtgacccacg gattaagagt ccgctgctct gccagctgag ctaaggaggc aaagaaaaaa
     1561 gctgtattgg tgccgaaact tcacggtttg tattgaaccc gcgcaattaa gcaggtgggc
     1621 aactcgctct aactgaagct gtttccgtgt gagacggcct acatgctgtt agaagacttt
     1681 tgtttcccta ataatacaaa aaatagtcgg tcaacactta agtgtgaagt cgtacaccac
     1741 agcgtttcta tatttatatg ataccacttt tttaaaaaaa atcaagagga aagtgcaatt
     1801 ttttgaaaag gatttcagat ttttcgcaaa cggtcgatag cttcctttct ttgagccaaa
     1861 gcccgttcat atttaccagt atcttctgct tggaaatagt gatgattacg aattttttct
     1921 ggcagatagt cttgcttgac ccaatttcca ggatagttgt gtggatagag atagtcttgg
     1981 gcattctcca gttccttgct tccactgtag tgcccatcac gtaggtgtcg cggaataggc
     2041 aagtgccctg atgttttgag gtcagcaagt gccttatcca tagctacata ggctgagttg
     2101 gattttggag aaagggccaa atcaatcacg acattggcaa tgagaatgcg ggcttctggg
     2161 aacccaatct tctgggcagc atccagagca gtcacggtat gaatctgggc ctcaggattg
     2221 gctaagccga tatcttcata ggcgataaca gtcaagcgac gagcgagact aggcagatcc
     2281 ccagcctcaa tcaagcgggc agcatagtgg agactggcat ccacatctga gccacggata
     2341 gacttttgca gggcagatag aacatcatag tgaccgtctc catccttgtc catagtgatg
     2401 tagcttctct gaagactatt ttccatgatg tctaaggtga tgtggcgaat gccctcgtca
     2461 ttctcagggg tagagagaac agccaagtcc agtgagttaa aggcagagcg aaggtctccg
     2521 tttgtagagg ttgcgatgaa atccagcgca tcctcatcta gttctattgg aaaatcaaaa
     2581 ccacgttcag ggttacttag agttatctgc agggcctctt tgacgtcttg gttagacaga
     2641 ggttccaact cgaaaatttg aactcggcta cgaatggcag gagtgacaga gaagaaagga
     2701 ttttcagtcg ttgctccaat catgatgacc agtccacttt ccaagagagg caagaggaag
     2761 tcttgcttgg tcttatctag tcgatgaatt tcgtctagca ataggacgag accaccagaa
     2821 aatttagctt cttccgagat ttcttgcagt cgctttttac tatcaactgt cgcattgaaa
     2881 gttcgaaagg catacttggt cgttccagcg atggcagagg caatactggt tttgccgatt
     2941 cccggagggc catatagaat catggaggac aggcggttgg cttccaccat gcggcggatg
     3001 atttttccat gtccgaccag atgctcctga ccgatgacct ggtcgatggt tttagggcgc
     3061 atgcgaagcg cgagattgtc tggcatagca gtcctttcta acgtggattt gagtagaatg
     3121 actcatctac tatatcttac tatgtccatt ctagcctatt agttgtgata tttcaatggt
     3181 tttgtaataa tcagggagtg agatagaatc ttttttacta tttaatttaa tgaggagagg
     3241 tatcagcatg agagatttga ttttccttaa agctcaaaat aggatagttg agcctaaaga
     3301 agtggaagtg ctgacttgag aaataatatt gctgaatgtc attgtatgaa ctataatgaa
     3361 aagagaggtg ataagcaatg gaaatgatgg accaagtcag gaaattttga tttttacaaa
     3421 acaaattcgt cattggattc ttagtgatca agttatttca gggaaaagaa aacttttctt
     3481 ccgggaagat actccaaaag agattttaga tttgtacgaa aacattaaat ctaaacttga
     3541 ttttgcttac caa
//
LOCUS       DAVGJL010000142         3540 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.143, whole genome shotgun sequence.
ACCESSION   DAVGJL010000142 DAVGJL010000000
VERSION     DAVGJL010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.143"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            44..631
                     /locus_tag="VVE42_001882"
     CDS             44..631
                     /locus_tag="VVE42_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HEU3074024.1"
                     /translation="MTSYKRTFVPQIDARDCGVAALASIAKFYGSDFSLAHLRELAKT
                     NKEGTTALGIVKAADEMGFETRPVQADKTLFDMSDVPYPFIVHVNKEGKLQHYYVVYQ
                     TKKDYLIIGDPDPSVKITKMSKERFFYEWTGVAIFLATKPSYQPHKDKKNGLLSKLPS
                     SDFQTKISHCLHCSLKLIGHYYQYRWFLLSPRNLG"
     gene            624..2201
                     /locus_tag="VVE42_001883"
                     /pseudo
     CDS             624..2201
                     /locus_tag="VVE42_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864064.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
     gene            2212..>3540
                     /locus_tag="VVE42_001884"
     CDS             2212..>3540
                     /locus_tag="VVE42_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HEU3074025.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVLIIPLSLLFTFILIFSLVATKE
                     ITVTSQGEITPTSVIASIQSTSDNPILANHLVANQVVEKGDLLIKYSETMEESQKTAL
                     ATQLQRLEKQKEGLGILKQSLETTTDLFSGEDEFGYHNTFMNFTKQSHDIELGITKTN
                     TEVSNQANLSNSSSSAIEQEITKVQQQIGEYQELRDAIINNRARLPTGNPHQSILNRY
                     LIASQGQTQGTAEEPFLSQINQSIAGLESSIASLKIQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLDQATQRLENNTLTSPSKGIVHLNSEFEGKNRI
                     PTGTEIAQIFPVITDTREVLITYYVSSDYLPLLDKGQTVRLKLEKIGNHGTTIIGQLQ
                     TIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTAKKTYFDY"
BASE COUNT         1171 a          724 c          585 g         1060 t
ORIGIN      
        1 ttatgacata atagacctat ccactatatg aaaggaattg ccaatgactt cttataaacg
       61 tacatttgtt cctcaaatag atgcgagaga ctgtggtgtc gctgccttag cctcgattgc
      121 taaattctat ggttcagatt tttctctagc tcacttgaga gaacttgcaa agaccaataa
      181 agaagggacg actgctcttg gcattgtaaa agccgctgat gaaatgggct ttgaaacaag
      241 acctgttcaa gcagataaaa cgctctttga catgagtgat gtcccctatc catttatcgt
      301 tcacgttaac aaagaaggaa aactccaaca ttactatgtt gtctatcaaa caaagaaaga
      361 ctatctgatt attggtgatc ctgacccttc tgtaaaaatc actaaaatgt caaaagaacg
      421 ctttttctat gaatggactg gagtagctat ttttctagct accaaaccca gctatcaacc
      481 ccataaagat aaaaagaatg gtctactaag caagcttcct tcctctgatt ttcaaacaaa
      541 aatctctcat tgcttacatt gttctctcaa gcttattggt cactattatc aatataggtg
      601 gttcttacta tctccaagga atcttggatg aatacattcc aaatcagatg aaatcaactt
      661 taggaatcat ctcagttggt ctggttatca cctatatcct ccaacaagtc atgagcttct
      721 ccagagatta tctcctaacc gttctgagcc agagattaag cattgatgtg attttatcct
      781 atattcgcca tatttttgaa cttcccatgt ctttctttgc gacacgtcgt acaggagaaa
      841 tcatttcacg gttcacagat gctaactcta ttatagatgc cttggcttct accattcttt
      901 ctctttttct ggatgtttct attctgattc ttgtaggggg agtcttgctg gcacaaaacc
      961 ctaatctctt cctcctttct cttctttcca ttcctatata catgttcatc atcttttctt
     1021 ttatgaaacc ttttgaaaaa atgaaccatg atgtcatgca aagtaattct atggttagct
     1081 ctgccattat cgaagatatc aacgggattg aaactataaa gtcgctcacg agtgaagaaa
     1141 atcgctatca aaatatagac agcgaatttg tagattattt ggaaaaatcc tttaagctca
     1201 gtaaatattc tattttacaa acgagtttaa agcagggaac aaaattagtt ctgaatatcc
     1261 ttatcctatg gtttggcgct caattagtca tgtcgagtaa aatttctatc ggtcagctga
     1321 ttacctttaa cacacttttt tcttacttta caactcctat ggaaaatatt atcaacctcc
     1381 aaaccaaact ccaatctgcg aaggtcgcta ataaccgttt gaacgaagtc tatctagtcg
     1441 aatctgaatt tcaagttcaa gaaaaccctg ttcattcaca ttttttgatg ggcgatattg
     1501 aatttgatga cctttcttat aagtatggtt ttggacgaga taccttaaca gatattaatc
     1561 tcacgattaa acaaggagat aaggttagcc tagttggagt tagtggttct ggtaaaacaa
     1621 ctttagccaa aatgattgtc aatttctttg aaccctacaa agggcatatt tccatcaatc
     1681 attaggatat taaaaacatt gataaaaaag tcttgcgccg tcatattaat tacctacccc
     1741 aacaagccta tatctttaat ggctctatct tggaaaactt aaccttgggc ggtaatcata
     1801 tgattagcca agaagatatt ctaagagctt gtgaattagt tgaaattcgt caagacattg
     1861 aaagaatgcc tatgggctat caaactcagc tctctgatgg agctggtcta tcaggaggac
     1921 aaaagcaacg aatagccctc gctcgtgctc ttttaactaa atctcctgtt ttaatactag
     1981 acgaagctac tagcggtctt gatgtcttga ctgagaaaaa ggttatagat aatcttatgt
     2041 ccctaactga taaaaccatt ctctttgtag cccatcgtct cagtatagcc gaacgaacta
     2101 accgtgtcat tgttcttgac caggggaaaa tcattgaagt tggtagtcac caagagttaa
     2161 tgcaagcgca aggattctac catcatctat tcaataaata aggagaatgt catgaatcct
     2221 aatcttttta gaagcgtcga gttttatcag agacgttacc ataactatgc gacagtgtta
     2281 attatacctc tttcattact atttactttc atcttgattt tctcccttgt tgccacaaaa
     2341 gaaattactg ttacttccca aggggaaatc acccctacaa gtgtcattgc atctattcag
     2401 tcgaccagtg ataatcctat ccttgctaat catttagtgg caaatcaagt agttgaaaaa
     2461 ggggacttac tcatcaaata ctctgaaaca atggaagaaa gtcagaaaac tgccttagca
     2521 actcaattac aaagacttga gaagcaaaaa gaaggacttg gaattttgaa acaaagctta
     2581 gaaacaacga ctgatctttt ttctggcgag gatgaatttg gctaccataa tacctttatg
     2641 aattttacta aacaatccca tgatattgaa ctgggtatca caaagactaa cactgaagtt
     2701 tcaaatcaag ctaatctttc caatagcagt tcatcagcta ttgaacaaga aattacaaaa
     2761 gttcaacaac aaattggaga atatcaagag ttgagagatg ctatcataaa taacagagca
     2821 cgcttaccaa ctggcaatcc gcaccagtca attttaaatc gttatcttat agcctctcaa
     2881 ggacaaacac aaggaactgc agaggagcca tttttatctc aaattaatca aagtattgca
     2941 ggtcttgaat catctatcgc aagcctcaaa attcagcaag ctggtatcgg aagtgtagca
     3001 acttatgata acagtttagc aaccaaaatt gaagtactcc gcactcagtt tttacagaca
     3061 gcctcacagc aacaactaac cgtggagaac caattaacag aattaaaagt acaactagat
     3121 caagccacac agcgtttgga aaacaatacc ttaacctccc caagtaaagg tatcgttcat
     3181 ctgaacagcg aatttgaagg taaaaataga attccaactg gtacagaaat tgctcaaata
     3241 ttccctgtca tcacagatac aagagaagta ctaatcactt actacgtatc ttctgactat
     3301 ctacctctac tagataaagg acaaactgta agattaaaac tggagaagat tggaaatcac
     3361 ggcaccacca tcatcggcca acttcagaca attgatcaaa ctcctaccag aacagagcaa
     3421 ggaaatctct ttaaattaac cgctcttgca aaactatcta acgaggatag taaactcatc
     3481 caatatggct tacaaggtcg cgtcactagt gtaactgcaa agaaaacata ttttgattat
//
LOCUS       DAVGJL010000143         3496 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.144, whole genome shotgun sequence.
ACCESSION   DAVGJL010000143 DAVGJL010000000
VERSION     DAVGJL010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3496
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.144"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            17..1426
                     /locus_tag="VVE42_001885"
     CDS             17..1426
                     /locus_tag="VVE42_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEU3074026.1"
                     /translation="MKKKLVFPNLFWWGSASSGPQTEGQYGKVHENVMDYWFKTHPED
                     FFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFY
                     NAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
                     HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLGLDG
                     KIGIILNLTPAYPRSNSPEDLEASRFTDDFFNKVFLNPAVKGTFPERLVKQLERDGVL
                     WSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMPDQYFKEYKWPERRM
                     NPYRGWEIFPKAIYDIAMIVKEEYGNIPWFISENGMGVENEARFIGENGVIDDVYRIE
                     FYEEHLRWLHKAIEEGSHCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKSS
                     GRWYRKVSDNNGFEVEIEE"
     gene            1444..2739
                     /locus_tag="VVE42_001886"
     CDS             1444..2739
                     /locus_tag="VVE42_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU3074027.1"
                     /translation="MKNSKFIDQFATFAGKLGNQIHLKTLRDAFVTVMPLYILAGLIV
                     LLNNTVFKWIFQGDTLTRFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPLAA
                     SMLSLVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGENIPPAVSRSFSVLLPVMTVISLFGVVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIGVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFALMGGSGSTLCLLIATFLVSRNAVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTGFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMEG"
     gene            2744..3052
                     /locus_tag="VVE42_001887"
     CDS             2744..3052
                     /locus_tag="VVE42_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU3074028.1"
                     /translation="MKQILLVCNAGMSTSMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNIPVDTIDMRDYGMMNGAKVLEQALAWIGEIR
                     "
     gene            3049..3357
                     /locus_tag="VVE42_001888"
     CDS             3049..3357
                     /locus_tag="VVE42_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU3074029.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEAHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
BASE COUNT         1172 a          597 c          685 g         1042 t
ORIGIN      
        1 agaaaagagg ttgagcatga aaaagaagtt agtatttcct aatctgtttt ggtggggatc
       61 tgcttctagc ggacctcaga cagaaggtca atatggaaaa gtacatgaaa atgtgatgga
      121 ctactggttc aaaacgcatc cagaagattt tttcgataat gtcggacctc ttgtagccag
      181 taactttttt catacttaca ccgaagattt ccacttgatg aaggaaattg gagttaattc
      241 tttccgcact tccatccaat ggagtcgact catcaagaat ttagagacag gtgagcctga
      301 tccaaaaggt attgctttct acaatgccat cattgaagaa gctaaaaaga accagatgga
      361 tcttgtgatg aatttacatc attttgattt accagtggaa cttcttcaaa aatacggtgg
      421 ttgggaaagc aaacatgtag tggagttatt cgtgaagttt gccaagactg ctttcacatg
      481 ctttggagat aaggttcatt actggacaac tttcaatgag ccaatggtca ttccagaagc
      541 aggatactta tatgctttcc attatccaaa tctaaaagga aagggaaaag aggccgtaca
      601 agtcatctat aatctaaacc ttgctagtgc aaaagtgatt caactatatc gctcattagg
      661 acttgatgga aagattggga ttattttaaa cttgacacct gcttatccaa gaagtaattc
      721 tccagaagac ttagaagcaa gtcgatttac agatgacttc tttaacaaag tcttcttgaa
      781 tccagctgtt aaaggaactt tcccagaaag attggtaaaa cagctagaga gagatggcgt
      841 gttatggagt cataccgaaa aagagcttca actgatgaaa tcaaatacgg ttgattttct
      901 tggagtaaac tactaccatc caaaacgtgt tcaagcacaa gcaaatcctg aggaatatca
      961 gacgccctgg atgccagacc aatacttcaa agagtataaa tggccggagc gtcgcatgaa
     1021 tccatatcgt ggttgggaaa tttttccgaa agccatttat gatattgcta tgattgtgaa
     1081 ggaagaatat ggtaatattc catggtttat cagtgaaaac ggaatgggtg ttgaaaacga
     1141 agcacggttt atcggtgaaa atggagttat cgatgacgtg tatcgtattg aattttatga
     1201 agaacattta agatggctac ataaagccat tgaagaggga agtcactgtt ttggatacca
     1261 cgcttggacc gcatttgatt gctggtcttg gaataatgca tataagaatc gttacggatt
     1321 tatctccgtt gatttagaaa cgcaaaagag aaccatcaag agctcaggaa gatggtatcg
     1381 caaagtaagt gacaataacg gttttgaagt agaaattgag gagtaaagag aggagatatt
     1441 cagatgaaaa actcaaaatt tatagatcaa tttgccacct ttgctggtaa actagggaac
     1501 caaattcatt taaaaactct aagagatgca ttcgtaacag taatgccatt atatattttg
     1561 gcaggtttaa tcgttctttt gaacaacacg gtatttaagt ggattttcca aggggataca
     1621 ttaacaagat tccaatattg gggaataaca attgcaaacg gtactttaag tatttcaggt
     1681 atgattattg ctgtaatggt tggttatttc ttagctaaaa acagagattt cgaaaacccg
     1741 ttagcagcat caatgctatc attagtttct ttaattgtga tgatgccaaa tacagtttct
     1801 gtagttcctg acggagcaaa agatgcggta aacatttcag gtgttctttc attcaacaac
     1861 acaggtacag gcgcaatgtt cgccggggtt atcgtagcga ttattgcaac agaattattc
     1921 attgaattat caaacgttaa agctttacaa atgaaccttg gtgaaaatat tccaccagct
     1981 gttagtagat catttagcgt attacttcca gtcatgaccg tcatctcctt atttggggtt
     2041 gtttcagcat tattattcaa tataactgga atgaacttaa tctcaatcat tacaatcttt
     2101 attcaagaac caattcgtca tattggtaca agcttaatcg gggtcattat tatttactct
     2161 ttaggaaata tgttatggct atttggtatt caccaagcag ttatttacag tgccatccta
     2221 gaaccattac tattaattaa cattactgaa aacatcactg cagcaaataa tggacaagcc
     2281 attccacaca tcatcaacct atcacaaata caaacattcg ctttaatggg tggtagtgga
     2341 tctacattat gtttattaat agcaacattc ttagtgagtc gcaatgctgt ctctaaaaac
     2401 gtggctaaat tatcttttgg acctggtatc ttcaatatca atgaaccagt attattcggt
     2461 tacccaatcg tttataacat ttcattagct attccattta tcacagttcc agtccttggt
     2521 attttaatca gctacttagc aacagttaca ggattcatga gtcctgcatt tatacaagtt
     2581 ccttggacta caccagtatt cttaaatgca tggttagcaa cagcagggga cgtgagagca
     2641 gttctagttc aattcatcat ctttgcactt ggagttcttc tatacattcc atttatcaaa
     2701 gttaatgaca aagttgttga acaagaaatg gaaggttaag attatgaaac aaattttatt
     2761 agtatgtaat gcgggaatgt cgacaagtat gcttgttaaa aaaatgcaac aaagtgcgac
     2821 tgaacgtggg attgaaatct ctatccaagc aaaatcaatg acagaagcta aaaagaatat
     2881 ccatgaagca gatgtcattc taattgggcc acaaattcgt tatgagttac ttgctgtgaa
     2941 agaaattgca ggaaatatcc ctgtagatac cattgatatg cgggattacg gaatgatgaa
     3001 tggagcaaaa gttctggaac aagcacttgc atggatagga gaaataagat gacagaagaa
     3061 atggaatata tctgttttca gctcattgcc aatagtggag cagccaaatc atcctttatt
     3121 gaagccatcc aactagctaa ggcaggaaat ttaaaagaag cgaaaattaa agttgaagaa
     3181 gcggaagact ccttagtaga agcgcacaag attcactcta atctcataca aaaagaagct
     3241 actggagaaa aaataggatt ctccttgcta tttatgcatg cagaagacca aatggcttca
     3301 acagagatta ttcagttgct atctaaagaa ttcatcgatc tctatcaaaa taaataggaa
     3361 cactgtcatt cccatctgtc tttattaatg acgactacat tgtaggaacc tccaaaagtt
     3421 ggacaaaatg attgaaagga ttggatcctg cattacacaa gatccaatcc ttttctttta
     3481 ctctagtttt tataat
//
LOCUS       DAVGJL010000144         3496 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.145, whole genome shotgun sequence.
ACCESSION   DAVGJL010000144 DAVGJL010000000
VERSION     DAVGJL010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3496
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.145"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(175..786)
                     /gene="rpsD"
                     /locus_tag="VVE42_001889"
     CDS             complement(175..786)
                     /gene="rpsD"
                     /locus_tag="VVE42_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEU3074030.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(981..2033)
                     /locus_tag="VVE42_001890"
     CDS             complement(981..2033)
                     /locus_tag="VVE42_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEU3074031.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVAKLEKDQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            complement(2030..2728)
                     /locus_tag="VVE42_001891"
     CDS             complement(2030..2728)
                     /locus_tag="VVE42_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU3074032.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGGETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQT"
     gene            complement(2895..>3496)
                     /locus_tag="VVE42_001892"
     CDS             complement(2895..>3496)
                     /locus_tag="VVE42_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat-containing
                     protein"
                     /protein_id="HEU3074033.1"
                     /translation="ASDKAPWSDNGEAKNPALSPLGENVKTKGQYFYQVALDGNVAGK
                     EKQALIDQFRANGTQTYSATVNVYGNKDGKPDLDNIVATKKVTIKINVKETSDTANGS
                     LSPSNSGSGVTPMNHNHATGTTDSMPADTMTSSTNTMAGENMAASANKMSDTMMSEDK
                     AMLPNTGETQTSMASIGFLGLALAGLLGGLGLKNKKEEN"
BASE COUNT          952 a          785 c          680 g         1079 t
ORIGIN      
        1 aggattatac cacattgtgt actatttttg gttcatttta ctatatcact ttggactata
       61 tcggtctagt tatgtctcaa cgattatagc tggatactaa gagaaatcaa aaaagcacta
      121 agggagaacg tcccaaagtg cttaatatca aagcctatct tattcaataa aatattacaa
      181 cattttgttg tagaattcaa cgacaagtgc ttcgttgatt tctgggttga tttcgtcgcg
      241 ttctggcaag cgagtcaatg aaccttccaa tttttcagcg tcgaatgata cgaatgctgg
      301 acgtccaaga gtagcttcta ctgcttcaag gattgctgga actttcaatg atttttcacg
      361 aactgagatc acttgacctg gagttacgcg gtatgatggg atatcaacgc gtttcccgtc
      421 aacaaggatg tgaccgtggt ttacgaattg acgagcttga cgacgagtag tcgcgagacc
      481 aagacggtaa acaacgttat ccaaacgacg ttccaaaaga agcataaagt tgaaacctag
      541 gattccgcct ttgatttttg tagcttgtac gaacaagtta cggaattgtt tttcacctac
      601 accgtaagtg aaacgaagtt tttgtttttc agccaattgc aaaccgtatt ctgacaattt
      661 agaacggttg tttggtccgt gttgtcctgg tacgtagtta cgacgtgcca attctttacc
      721 tgtacctgta agtgaaaggc caaggcgacg agcttgtttc caagatggtc ctgtataacg
      781 tgacatatga atgtcctcct gatataaata atatttcggt ggaaatagtc acttagaaag
      841 ccctgattcg tgcagatgcc cttcgcctaa acagccaagg ttacttgtca taagacacct
      901 gttgacgagc ttcatgcttt cctgctgata tttcacacaa aatctattct accatgaata
      961 gctggatttg taaaggggtt ttaggcttta ttttcactac cagagaggtt gagaacgagg
     1021 gtaaaggtac ttcctagacc gtactggctg ctgactgtga tttccccacc caattgatgg
     1081 gccaattcac gcgcaatcgc aagtcctaat ccatgaccac ctgtcttcat gttacgcgaa
     1141 gtttcgacac gataaagacg tttgaaaata ttttccaaat cctctggggc aataccctgc
     1201 ccttcatcgg tcacactgat tgaaagctgg tccttctcca gcttagccac cacttccagc
     1261 ttggttcctg gagcagaata tttaaaagcg ttatcgacca gattcaccaa gatacgagaa
     1321 agcttagcat aatctccctc aatccgggca gactctggga ttacctgcaa gtggacatct
     1381 cttctctcct gctcaatcaa aaactgaaat tcactcatgc actcaattaa gagcttgtcc
     1441 agaaaaatac tgtctttact ggtagtttcc acctgatttc tagctgtgtt tagggtcaaa
     1501 aaattcaact cctcaaccag tttattgagc ctctccgtct ggcgtccaat ggttgctaga
     1561 taatgagctt gctccgactc cttgataatc ccatccaaaa tcccttctac cgtcgcttgg
     1621 atcgaagtga taggagtctt aatatcatgc gacaactgag caatcatcaa gcccttttct
     1681 cgttcgcttt cttccaagga atcaaagctt acctgcaaat catgggacat ctcattaaaa
     1741 gtttgcccta attgctgaaa ttctacagga ccttgaacct ccaaatttga aggaaaatcc
     1801 ttggccgcta cccgcttggc atgctccttg agtttgccca acgacgtaaa gactggcaat
     1861 aggagaaaga gactaatccc agcaccgaca aggctggcaa cgatggtcat cccaagcaaa
     1921 aagtaaatct cgcctttcgc aatcagcatt ttttgaacag cccaaaaaac aaccaaaatg
     1981 gttaagaggg ttgaaataat atatccaacc aaaatataac tttttagttt catgtttgtc
     2041 ctctcggttt ctctatctta tatcccaacc cccaaactgt cttaatagtg ggcgtttggt
     2101 cactactata ttttgccagc tcctgtcgaa gagcatggat atgcacattc aaggtattgg
     2161 tgtcatccac gtagtcttct ttccagatct tttcatagag gtctgtcttg gagaaaactc
     2221 gctctggatt gctagctaaa atccacagca attcaaatga tttaacagtt aaatccagca
     2281 tttcttctcc tatttgaact tcatgactac tatgattcat ttttagattg ccaagggaaa
     2341 tcagctctgt ttcgccccca cgatgaaggc ggcgcaaaat attgtggaca cgcaaaacca
     2401 gctcacgtgg gctaaaaggc ttagcaataa aatcatctgc tcccaagctc aggccgtaaa
     2461 tcttgtcctg ttcactggtc ttagcagtaa taaataggaa aggctgctct ggtgataagt
     2521 attgaacctc actgattaaa tcataaccat ccatccgagg catcatgaca tctgtgataa
     2581 tcaaatcaat cggttttttc ttgaacagct ctagcgcttc cagtccatca tgggctacca
     2641 agacctgata acctgcctga attaagtatc tctgatgaat atctgtgatt tctacctcgt
     2701 cgtcaacgag taaaattgtc tttcccatct gtctctcctt tgataaaaac agtgctatac
     2761 tgctttaagt ataacactat ttttattgtt ttacgatgat tttaatctgc tattgaaaga
     2821 aagtcctgat tgctgattga gttgttaaac taatcttagt cagcccacta tccatcattt
     2881 atttccttag ctgattagtt ttcttctttt ttgtttttca aacctagacc accgagtaaa
     2941 cctgcaagcg caagcccaag gaaaccaata cttgccattg atgtttgagt ctcaccagta
     3001 tttggtagca tagctttatc ctctgacatc atcgtatcag acatcttgtt agcagaagca
     3061 gccatgtttt cacctgccat cgtgttggta gaacttgtca tggtgtcagc aggcatgcta
     3121 tctgtagtac ctgtagcatg attgtgattc atcggagtca cgccagaacc agagttagaa
     3181 ggtgataatg aaccatttgc tgtgtctgat gtttctttaa catttatctt aatagtgact
     3241 tttttagttg ctacgatgtt gtccaagtct ggtttaccgt ctttgttacc atagacattg
     3301 actgtagcgc tgtaagtttg agtaccgttt gctcggaact ggtcaatgag cgcttgtttt
     3361 tctttgccag ctacatttcc gtccaaggct acttgataga agtattgacc tttggtcttc
     3421 acgttttcac ctagtggaga tagggctggg tttttagcct caccgttatc tgaccatggt
     3481 gccttgtcag atgcct
//
LOCUS       DAVGJL010000145         3494 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.146, whole genome shotgun sequence.
ACCESSION   DAVGJL010000145 DAVGJL010000000
VERSION     DAVGJL010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.146"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..991
                     /locus_tag="VVE42_001893"
     CDS             <1..991
                     /locus_tag="VVE42_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3074034.1"
                     /translation="QIKYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTLRALVGFL
                     DPSSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKKVAPDVIK
                     AKVSAVAAKIKISDQQLQRNVSELSGGQQQRVALARALVLEPKILCLDEPLSNLDAKL
                     RVDLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPVEIYHNSQ
                     TEFVCDFIGDINVLTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQDFILKGT
                     IIDVEFSGVTIHYTIKVSESQILNVTSIDSQAAIRSVGESVELFITPSDVLQF"
     gene            1003..2694
                     /locus_tag="VVE42_001894"
     CDS             1003..2694
                     /locus_tag="VVE42_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU3074035.1"
                     /translation="MRHKLNLKDWLIRLGLIWFLVTFIIYPNFDLVVNVFVKGGEFSL
                     DAVHRVLKSQRALQSIMNSFKLAFSLIITVNVVGILCVLFTEYFDIKGAKILKLGYMT
                     SLIYGGVVLATGYKFVYGPYGLITKFLQNVIPSLDSNWFIGYGAVLFIMTFSGTANHT
                     LFLTNTIRSVDYHTIEAARNMGAKPFTVFRKVVLPTLIPTLFALTIMVFLSGLSAVAA
                     PMIVGGKEFQTINPMIITFAGMGNSRDLAALLAIILGIATTILLTIMNKIEKGGNYIS
                     ISKTKAPLKKQKIASKPWNIIAHIVAYGLFTVFMLPLIFIVLYSFTDPVAIQTGNLTL
                     SNFTLENYRLFFSNSAAFSPFLVSFIYSIIAATTATILAVVFARVVRKHKSRFDFLFE
                     YGALLPWLLPSTLLAVSLLFTFNQPQFLVLNQILVGSLVILLIAYIVVKIPFSYRMVR
                     AILFSVDDEMEDAARSMGASPFYTMMKVIIPFILPVVLSVIALNFNSLLTDFDLSVFL
                     YHPLAQPLGITIRSAGDETATSNAQALVFVYTIVLMIISGTVLYFTQRPGRKVRK"
     gene            2697..3407
                     /locus_tag="VVE42_001895"
     CDS             2697..3407
                     /locus_tag="VVE42_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEU3074036.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EAYNRVLVYCAENGIRMTDSRTAFGDVNSDRIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
BASE COUNT         1067 a          513 c          664 g         1250 t
ORIGIN      
        1 tcaaattaaa tatggtgatt ttgttgcaat tgataatctg aatttagata tacatgaagg
       61 ggaattcttt acatttcttg ggccttcagg atgtggtaaa tcaactactt tgagagcatt
      121 ggtaggtttt ttagatccat catcaggaag tattgaagtt aatggaacag atgtcactca
      181 tttggaacct gaaaagcgtg gaattggtat tgtatttcaa tcttatgcgc tatttccaac
      241 tatgactgtt tttgataata ttgcatttgg tttaaaagtt aagaaggtag ctccagatgt
      301 tattaaagct aaagtatcag cagtggcagc aaaaattaag atctctgatc aacagttaca
      361 gcgtaatgta tcagaattat ctgggggtca acaacaaagg gtagcattgg ctcgtgctct
      421 ggttcttgaa cctaaaattc tttgtctaga tgaaccattg tcaaaccttg acgcaaaatt
      481 acgtgtagat ttgagaaaag agttgaaaag acttcaaaaa gagttaggta ttactacttt
      541 atatgttact catgatcaag aggaagcctt gactttatct gatagaattg cagtctttaa
      601 caatggatac atcgaacagg tcggtacacc agtagagatt tatcataatt ctcaaactga
      661 atttgtatgt gattttattg gagatattaa tgttttgacc gatgaaacag tccacgaagt
      721 attattgaaa aatacaagcg ttttcttaga ggataaaaaa ggatacattc gattagagaa
      781 agttcgattc aatcgtgaaa ctgaacaaga ttttattcta aaagggacaa ttattgatgt
      841 tgagttttct ggagttacaa ttcactatac aataaaagtt tctgaaagtc agattcttaa
      901 tgtaacaagt attgatagtc aggctgctat tagatctgtc ggagaaagtg tggaattatt
      961 tatcacacca tcagacgttc tgcaatttta aggaggtgca gtatgcgtca taaattaaat
     1021 ttaaaagatt ggcttattcg tttagggtta atctggttct tagtaacatt tattatttat
     1081 ccaaactttg atctagtagt gaatgtattt gtaaaaggag gagaattttc ccttgatgct
     1141 gtacatcgtg ttctaaaatc tcagagggca cttcagagta ttatgaacag ttttaagtta
     1201 gcattttcac tcattattac agttaatgtc gtaggtattc tttgtgttct atttacagag
     1261 tactttgata ttaaaggtgc taaaatttta aaattaggtt atatgacctc tttaatttat
     1321 ggaggagtgg ttttagcgac tggatataaa tttgtctatg gtccatatgg attgattaca
     1381 aaatttttac aaaatgttat cccttcttta gactctaact ggtttattgg gtatggtgca
     1441 gtcttattca ttatgacatt ttcaggaact gctaatcata cattgttttt aacaaataca
     1501 attcgaagcg ttgactatca cactattgag gctgctcgaa atatgggagc aaaaccattt
     1561 actgttttcc gaaaagtagt gttaccaacc ttaattccaa ctctatttgc acttactatt
     1621 atggtttttc ttagtggttt atctgcagta gcagcaccca tgattgttgg tggtaaagaa
     1681 tttcaaacta taaatccaat gattattaca tttgcaggga tggggaattc tcgtgattta
     1741 gctgccttac ttgcaattat tttaggtatt gcaactacaa ttttgcttac tatcatgaat
     1801 aagatagaaa aaggtggaaa ttatatttct atctctaaga ctaaagcgcc tcttaaaaaa
     1861 caaaaaattg cgtctaagcc ttggaatatc attgctcaca ttgtagcata tggattgttc
     1921 acagttttca tgcttccact aatttttata gtattatact catttacaga tccagttgca
     1981 attcaaacag gtaacttaac attatcaaac tttactttag aaaattatcg cttattcttt
     2041 agtaatagtg cggcattctc tccattcttg gtcagcttta tttattctat tattgctgcg
     2101 acaacagcaa caattctcgc agttgtattt gctcgtgttg tcagaaaaca taaatctcgt
     2161 tttgatttct tatttgaata tggtgctcta cttccttggt tactaccaag tacactttta
     2221 gcagtaagtt tattatttac ttttaatcag ccacaatttc ttgtcttgaa tcagattttg
     2281 gtaggtagtt tggtaattct acttattgca tatatagttg taaaaatccc attttcttat
     2341 agaatggtac gtgctatttt atttagtgtt gatgatgaga tggaagatgc agcaagaagt
     2401 atgggtgctt caccttttta tactatgatg aaggttatca ttccatttat tttaccggtt
     2461 gttctctctg ttattgcttt aaactttaac tctttattaa ctgacttcga cttatctgta
     2521 ttcctttacc atcccctagc tcaaccatta ggtattacga ttcgatctgc aggtgatgaa
     2581 acagcaacat ctaatgcaca agctctggta tttgtttata caattgttct gatgattatt
     2641 tctggaacgg tattatactt cacacaaaga ccggggcgta aagtaaggaa ataatcatga
     2701 cagccactag tcttgggtta tcaaatattg aaatagttgt caggattgtt ttatcagtag
     2761 tcattggtag tataattggt ttagagagag ggagcaaatc ccagcctgca ggcatccgaa
     2821 cttatagtat tgtttgtcta gctgcatgtt tgattatgat gacgaatgaa tacgtatctt
     2881 ataaatttgg gacaggagat cctacacgat taggagctca agttatatca ggtgtgggtt
     2941 ttctaggcgc tggaacgatt cttattacag ataaaaagaa aattacaggt ctgacaactg
     3001 cagcaggcat ttgggcttcg gcaggaattg gattagctat tggagtaggt ttttatgagg
     3061 gagctctttt agtagccatt tctgtttggg gtgtgatatc catgttccaa ccactaaaaa
     3121 aatatctaca aaatcgttct aaaatgattg aattgtatat agtggttaaa tctacagaag
     3181 cctataatcg tgtattggtt tattgtgcag aaaatggtat cagaatgacc gattcaagaa
     3241 cagcgtttgg agatgttaat tctgacagaa ttgaatactt tgatgttccg gacaaaaaaa
     3301 tagcgtcatt tattactctg gaattatcag gtagatttga acatcttcga ctgatggaag
     3361 aaattgctaa tattgtaggt gttatttatg tcgaagaaat tagctgaaat ttagtaaaca
     3421 aagggacaga tgtttttgtc cttttgtttt ttagatgttt taatcatcta atttttgagt
     3481 agatttttgc tcag
//
LOCUS       DAVGJL010000146         3278 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.147, whole genome shotgun sequence.
ACCESSION   DAVGJL010000146 DAVGJL010000000
VERSION     DAVGJL010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.147"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(<1..1546)
                     /locus_tag="VVE42_001896"
     CDS             complement(<1..1546)
                     /locus_tag="VVE42_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEU3074037.1"
                     /translation="MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRF
                     LIEKGFHLIQGVYQDQGYLVRNLFVLVLFYILICWLSAKLTRSEKDIKGSGIPQVEAE
                     LKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
                     RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFG
                     LTPVLDMPDNIPPMTLDQYWIYLAMGIFLGFSGFLYEKAVLNVGRVYDLIGQKIHLDR
                     AYYPILAFILIIPVGIFLPQIIGGGNQLVLSLTEQNFSFQVLLAYFLIRFIWSMISYG
                     SGLPGGIFLPILALGSLLGALIGAICVNLGLVSQEQFPIFVILGMSGYFGAISKAPLT
                     AMILVTEMVGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSSEGEVTL
                     IEIPVSDKIAGKQVHELNLPHNVLITTQVHNGKSQTVNGSTRMYLGDMIHLVIPKSEI
                     GKVKDLL"
     gene            complement(1562..2341)
                     /locus_tag="VVE42_001897"
     CDS             complement(1562..2341)
                     /locus_tag="VVE42_001897"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEU3074038.1"
                     /translation="MATIKEIKELLVTVKELESPIFLELEKDNRSGVQKEISKRKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIAGIDEVGRGPLAGPVVAAAVILPKNCKIKG
                     LNDSKKIPKKKHLEIFQAVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFPGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKKES"
     gene            complement(2328..3179)
                     /gene="ylqF"
                     /locus_tag="VVE42_001898"
     CDS             complement(2328..3179)
                     /gene="ylqF"
                     /locus_tag="VVE42_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEU3074039.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LIKIVGDKPKLLILNKADLADPAMTKEWRQYFESQGIQTLAINSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIQIETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEEAPVIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EDLDGND"
BASE COUNT          950 a          692 c          662 g          974 t
ORIGIN      
        1 acaacaaatc tttgactttt ccaatttcac tttttggaat aaccaggtga atcatatcac
       61 ccagatacat tctggttgag ccgttaactg tttggctctt gccattatgg acttgagttg
      121 tgatgaggac gttgtgtggt aagttgagtt catgaacttg tttcccagca attttatcag
      181 aaactggtat ttcgataagt gtaacttctc cttcgctaga tacttcttct ggaagcattt
      241 tttccagcat ggcttcatag actggcgtac ctttgagcaa atccatgata atataagaaa
      301 caagagtgac aagaccaagt ggcataaggt tgcgaatatc tcctaccatc tcagttacga
      361 ggatcatagc ggttaaggga gcttttgata tggctccaaa atagccactc attcctagaa
      421 tgacaaatat agggaattgc tcttgactga caagtccaag attgacacag atagcaccaa
      481 ttaaggcacc aagcaaagaa ccaagagcta aaatggggag gaaaattcct cctggcagtc
      541 cacttccata gctaatcata ctccaaataa agcggatcaa aaagtaagct aataaaactt
      601 ggaaactaaa attttgttca gttaaagaaa ggacaagctg atttccgcca ccaatgattt
      661 gaggtaagaa gattccgact ggtatgataa ggataaaagc caagatggga taataagctc
      721 tatccaaatg gattttttga ccaatcaagt cataaactct tccaacgttt aatacagctt
      781 tctcatagag aaagcctgaa aatccaagga aaattcccat ggcgagatat atccaatact
      841 gatctagggt catgggagga atgttatctg gcatatccaa tactggtgtc aaaccgaaca
      901 tgagtagaga cacaaagttt gctacgatgc tggctgctag agttgagacc cagaaaaagc
      961 gcgaaaaatg gtgatagact tcttctacaa caaagagaag tgctgcaata ggagcattaa
     1021 aggctgcggc taaacctgct gcagctccac tggcaatcaa agagcgttcc tctactggac
     1081 tggatttgag ccacttggca attcctttac caccaactgc tccaagttga atactgggtc
     1141 cctctcgacc cagcatgagt ccactggcaa tagcaagaat acctagcaca tattttttcc
     1201 aaagaatgcc ccaccagttg agggacatga ggcctttcag ttcggcttcg acttgaggaa
     1261 ttcctgagcc tttaatatct ttttctgacc gtgttagttt ggcactgagc caacagatga
     1321 gtatataaaa caaaaccagt acaaaaagat tgcgcactaa gtacccttga tcttgataaa
     1381 ctccttgtat caggtggaag cccttttcaa ttaagaaacg aaaggatccg acgataattc
     1441 caacgatgag gccgacaatg attcctcggc caacttggga tagtatagtg ctggatgcaa
     1501 aggcgaattc tttcttagaa cggactattt cagactgttc ctccatggta tttcctttca
     1561 attaactttc ttttttacct aaaaccagtg atttaacggg ctcaaagctg gttcggtgaa
     1621 ttggggtaac tcctagtttt gtgaggcctt ccagatgttt agctgtgcca tatcctgcat
     1681 tagtagcgaa atcatagcca gggaactgct gatcgtattc cttcatcaat tcatcacgtg
     1741 ttaccttggc tactatagat gctgctgcga tagagaggga gttggcatct cctttgataa
     1801 tggaggtttg tgaaatgggc aagtccagtt tcatggcatc aatcaaaagg tgctctggtt
     1861 gagggctgag ctgggagatt gcttcttgca tggctagttt ggttgcttca tagatattga
     1921 cttggtcgat gacctgatta tctatgatac caattccaat cgacaaggct tggtcttgaa
     1981 cggcttggaa aatctccaga tgtttctttt taggaatttt cttgctgtcg ttgagacctt
     2041 taatcttaca atttttaggt aaaataacgg ccgcagcgac tacaggacca gcaagaggac
     2101 cacgaccaac ctcatcaata cctgcaatta aggtcaatcc ttgcttataa agttcttttt
     2161 cataagaaag catggattcc aagcgcaaat tttcatctaa ttcagcttgg atggctcttt
     2221 tacgcttgct gatttccttt tgaactcctg agcgattatc cttttcaagc tctaaaaaaa
     2281 tagggctttc taactccttg actgtcacaa ggagttcttt gatttcttta atcgttgcca
     2341 tcgaggtctt ccaatgtatc taaggtatag ttaccgagtt tgccatcacg gacttccttc
     2401 acgaagagac tgtaaaaacg gtcatagtca tcacggaaac cgagggcgcg ggtcatatcc
     2461 ataataatca caggcgcttc ttcttcaatt ttcatttgtt tgaagcgttc agccagcttt
     2521 tctggataat gttctttgaa ataattgata ccaaaaatgg taacctcatc cataggaagc
     2581 aactgatcct tgatagctcc agtcaatgcc aacttaagtg caacagtttc atcctcaaac
     2641 ttaggccaga gaatccccgg tgtatccaag atttccagat ctttattggt tttaagccat
     2701 tgttgacctt ttgtgacccc tggcttgttt ccaacaacag caatcttttt accagccaaa
     2761 cggttcatca gagttgattt accagcgttt ggaatcccaa taatcatggt acgcaaggtt
     2821 tcaatctgaa tcccacgttc tttctggcga gcaatcttat cagccatgag cttcttggcc
     2881 gcatctgtta caacttttac agtcacttgt tctttggagt tgatagctag cgtctggatt
     2941 ccttgtgatt caaaatactg acgccattcc ttggtcattg ctggatcagc caagtcggcc
     3001 ttgtttaaaa tcaagagttt tggtttatca ccaacaatct tgatcaacat aggattttga
     3061 ctagatagag gtaagcgtgc atcaactaaa atcgtcacaa aatcaacaaa ttttaaattc
     3121 tcctgcacct gtcgacgagc tttagacatg tgaccaggaa accattgaat agtagccatt
     3181 gagttttttc ctttcgtagc aaggatttag atcccctatt ttattttact atagtttgaa
     3241 catagagagt cttcaaatgg atgatgtcca aacagttc
//
LOCUS       DAVGJL010000147         3275 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.148, whole genome shotgun sequence.
ACCESSION   DAVGJL010000147 DAVGJL010000000
VERSION     DAVGJL010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.148"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            92..1579
                     /gene="tacF"
                     /locus_tag="VVE42_001899"
     CDS             92..1579
                     /gene="tacF"
                     /locus_tag="VVE42_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HEU3074040.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYNYGLRAISNVKDNKKDLNRTFSSLFYLCIACTILTTAVYILA
                     YPLFFIDNPIVKKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGGILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNHIVVPEYYLLTTML
                     SETSLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFVYPVDMVINLP
                     FLINTGLIVLLSAISYISLLVFTKDSIFYEFLNHVLALKNKFKKS"
     gene            1589..2392
                     /locus_tag="VVE42_001900"
     CDS             1589..2392
                     /locus_tag="VVE42_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU3074041.1"
                     /translation="MKQLTIEDAKQIELEILDYIDTLCKKHNINYIINYGTLIGAVRH
                     EGFIPWDDDIDLSMPREDYQRFINIFQKEKSKYKLLSLETDKNYFNNFIKITDSTTKI
                     IDTRNTKTYESGIFIDIFPIDRFDDPKVIDTCYKLESFKLLSFSKHKNIVYKDSLLKD
                     WIRTAFWLLLRPVSPRYFANKIEKEIQKYSRENGQYMAFIPSKFKEKEVFPSGTFDKT
                     IDLPFENLSLPAPEKFDTILTQFYGDYMTLPPEEKRFYSHEFHTYKLED"
     gene            2394..3203
                     /locus_tag="VVE42_001901"
     CDS             2394..3203
                     /locus_tag="VVE42_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU3074042.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKHDIPYFLSYGTMLGAIRH
                     KGMIPWDDDIDISLYREDYECLLKIIEEENHPRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDRFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNTPQYEGGVGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYMTPPSKEMQEWYSHSIKAYRKN"
BASE COUNT         1006 a          614 c          550 g         1105 t
ORIGIN      
        1 agatttgaaa cttcaaagtg attacttgac ttactgccct tcatttctta ttagctaact
       61 ttatgatata atgaaaaacg aggtaaattg aatgaaaagt ataaaattaa atgctctatc
      121 ttacatggga attcgtgtct tgaatattat ttttcccatc ctaactggaa cctatgtcgc
      181 gcgtgtcttg gaccgaactg actatggtta cttcaactca gtcgacacta ttttgtcatt
      241 tttcttgccc tttgcaactt atggtgtcta taactacggt ttaagggcta tcagtaatgt
      301 caaggataac aaaaaagatc ttaacagaac cttttctagt cttttttatt tgtgcatcgc
      361 ttgtacgatt ttgaccactg ctgtctatat cctagcctat cctctcttct ttattgataa
      421 tccaatcgtc aaaaaggtct accttgttat ggggattcaa ctcattgccc agattttttc
      481 aatcgaatgg gtcaatgaag ctctggaaaa ttacagtttt ctcttttaca aaactgcctt
      541 catccgtatc ctgatgctgg tctctatttt cttatttgtt aaaaatgaac acgatattgt
      601 tgtctataca cttgtgatga gtttatcgac gctgattaac tacctgatta gttatttttg
      661 gattaaaaga gacatcaaac ttgttaagat tcacctaagt gattttaaac cactctttct
      721 ccctctgaca gccatgttag tctttgccaa tgccaatatg ctcttcactt ttttagatcg
      781 cctcttcctc gttaaaacag ggattgatgt caacgttagt tactatacca tagctcagcg
      841 aattgtgacc gttatagctg gggttgtaac aggtgcaatt ggagtgagtg tgcctcgtct
      901 cagttactat ctggggaaag gagacaaaga agcctatgtt tctctggtta atagaggtag
      961 tcgaatcttt aacttcttta tcattccact gagttttgga ctcatggttt taggaccaaa
     1021 tgccatccta ctttacggta gtgaaaaata tatcggaggc ggcatcttga cctctctctt
     1081 cgcttttcgt acgattatcc tggccttaga taccattctt ggttcccaaa ttctctttac
     1141 caatggctat gaaaaacgta tcacagtcta tacagtcttt gctgggctac tcaatttggg
     1201 cttgaatagt ctcctctttt tcaaccatat cgtggttcct gaatactact tactgacaac
     1261 tatgttatca gagacttctc tacttgtttt ctatattatt ttcatccata gaaaacaact
     1321 catccacttg ggacatatct ttagctatac tgttcgatac tctctctttt cactttcctt
     1381 tgtagcaatt tatttcctga ttaatttcgt gtatcctgtg gatatggtca ttaatttgcc
     1441 atttttaatt aatactggtt tgattgtctt gctatcagct atctcttata ttagtctact
     1501 tgtcttcaca aaagatagca ttttctatga atttttaaac catgtcctag ccttaaaaaa
     1561 taaattcaaa aaatcatagg agtttaaaat gaaacaacta accattgaag atgccaaaca
     1621 aattgaatta gaaattttgg attatattga tactctctgt aaaaagcaca atatcaacta
     1681 tattattaac tacggtactc tgattggggc ggttcgacat gagggcttta tcccttggga
     1741 cgacgatatt gatttatcca tgcctagaga agactaccaa cgatttatta acatttttca
     1801 aaaggaaaaa agcaagtata agctcctatc cttagaaact gataagaact actttaacaa
     1861 ctttatcaag ataaccgaca gtacgactaa aattattgat actcgaaata caaaaaccta
     1921 tgagtctggt atctttatcg atattttccc tatagatcgc tttgatgatc ctaaggtcat
     1981 tgatacttgt tataaactgg aaagcttcaa actgctgtct ttcagtaaac ataaaaatat
     2041 tgtctataag gatagccttt taaaagattg gatacgaaca gccttttggt tgctccttcg
     2101 accggtttct cctcgttatt ttgcaaataa aatcgagaaa gaaattcaaa aatatagtcg
     2161 tgaaaatggg cagtatatgg cttttatccc ttcaaaattt aaggaaaagg aagtcttccc
     2221 aagtggtacc tttgataaaa caatcgattt accctttgag aatttaagcc ttcctgcacc
     2281 tgaaaaattt gatactattt tgacacaatt ttatggagat tatatgaccc taccaccaga
     2341 agaaaaacgc ttctacagtc atgaatttca cacttataaa ttggaggatt aggatgcaat
     2401 atttagaaaa aaaagaaatt aaagaaattc aactagccct gctggactat attgatgaga
     2461 cttgtaagaa acatgatatt ccttattttc tcagttatgg aaccatgctt ggagccatcc
     2521 gccacaaagg tatgattcct tgggatgatg atattgatat ttccctttat cgtgaggatt
     2581 atgagtgttt actgaagatt attgaagaag aaaatcaccc tcgctacaag gttctttcct
     2641 acgatacatc ttcttggtac ttccataatt tcgcatcgat tttggacact tctactgtta
     2701 tagaagacca tgttaagtac aagcgtcatg ataccagcct tttcatcgat gtcttcccaa
     2761 ttgatcgatt tacagacttg agcattgtcg acaagagcta taagtatgtg gctcttcgtc
     2821 aactagctta tatcaaaaaa tcacgagcag ttcacggtga tagcaaacta aaagattttc
     2881 ttagattatg tagctggtac gctctccgat ttgtcaatcc tcgctacttt tacaagaaaa
     2941 ttgatcaact agtcaaaaat gctgtaacca acactcctca atatgaagga ggagttggga
     3001 tcggtaagga agggatgaaa gaaatcttcc cagttgatac ctttaaagaa ctgattttaa
     3061 ctgagtttga gggccgtatg ttgcctgttc ccaaaaaata tgaccaattt ttaacccaga
     3121 tgtatggcga ttatatgaca ccaccatcaa aagaaatgca agagtggtat agtcatagca
     3181 ttaaagctta tcgcaaaaac tgattgaggg ggattataca aactactaag atagaggtta
     3241 ttcaaaaaca taattttagt agaaaatgaa atata
//
LOCUS       DAVGJL010000148         3262 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.149, whole genome shotgun sequence.
ACCESSION   DAVGJL010000148 DAVGJL010000000
VERSION     DAVGJL010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.149"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            80..3199
                     /gene="phtE"
                     /locus_tag="VVE42_001902"
     CDS             80..3199
                     /gene="phtE"
                     /locus_tag="VVE42_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HEU3074043.1"
                     /translation="MKFSKKYIAAGSAVIVSLSLCAYALNQHRSQENKDNNRVSYVDG
                     SQSSQKSENLTPDQVSQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSE
                     ELLMKDPNYQLKDADIVNEVKGGYIIKVDGKYYVYLKDAAHADNVRTKDEINRQKQEH
                     VKDNEKVNSNVAVARSQGRYTTNDGYVFNPADIIGDTGNAYIVPHGGHYHYIPKSDLS
                     ASELAAAKAHLAGKNIQPSQLSYSSTASDNNTQSVAKGSTSKPANKSENLQSLLKELY
                     DSPSAQRYSESDGLVFDPAKIISRTPNGVAIPHGDHYHFIPYSKLSALEEKIARMVPI
                     SGTGSTVSTNAKPNEVVSSLGSLSSNPSSLTTSKKLSSASDGYIFNPKDIVEETATAY
                     IVRHGDHFHYIPKSNQIGQPTLPNNSLATPSPSLPINPGTSHEKHEEDGYGFDANRII
                     AEDESGFVMSHGDHNHYFFKKDLTEEQIKAAQKHLEEVKTSHNGLDSLSSHEQDYPSN
                     AKEMKDLDKKIEEKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVG
                     IGHSHSNYELFKPEEGVAKKEGNKVYTGEELTNVVNLLKNSTFNNQNFTLANGQKRVS
                     FSFPPELEKKLGINMLVKLITPDGKVLEKVSGKVFGEGVGNIANFELDQPYLPGQTFK
                     YTIASKDYPEVSYDGTFTVPTSLAYKMASQTIFYPFHAGDTYLRVNPQFAVPKGTDAL
                     VRVFDEFHGNAYLENNYKVGEIKLPIPKLNQGTTRTAGNKIPVTFMANAYLDNQSTYI
                     VEVPILEKENQTDKPSILPQFKRNKAQENSKLDEKVEEPKTSEKVEKEKLSETGNSTS
                     NSTLEEVPTVDPVQEKVAKFAESYGMKLENVLFNMDGTIELYLPSGEVIKKNMADFTG
                     EAPQGNGENKPSENGKVSTGTVENQPTENKPADSLPEAPNEKPVKPENSTDNGMLNSE
                     GNVGSDPMLDPALEEAPAVDPVQEKLEKFTASYGLGLDSVIFNMDGTIELRLPSGEVI
                     KKNLSDLIA"
BASE COUNT         1223 a          541 c          632 g          866 t
ORIGIN      
        1 aataaccggt ttagaaaact atttaataaa gtaaaagaag ttgagaaaaa acttcatcat
       61 ttattgaaat gagggattta tgaaattcag taaaaaatat atagcagctg gatcagctgt
      121 tatcgtatcc ttgagtctat gtgcctatgc actaaaccag catcgttcgc aggaaaataa
      181 ggacaataat cgtgtctctt atgtagatgg cagccagtca agtcagaaaa gtgaaaactt
      241 gacaccagac caggttagcc agaaagaagg aattcaggcc gagcaaattg taatcaaaat
      301 tacagatcag ggctatgtaa cgtcacacgg tgaccactat cattactata atgggaaagt
      361 tccttatgat gccctcttta gtgaagaact cttgatgaag gatccaaact atcaacttaa
      421 agacgctgat attgtcaatg aagtcaaggg tggttatatc atcaaggtcg atggaaaata
      481 ttatgtctac ctgaaagatg cagctcatgc tgataatgtt cgaactaaag atgaaatcaa
      541 tcgtcaaaaa caagaacatg tcaaagataa tgagaaggtt aactctaatg ttgctgtagc
      601 aaggtctcag ggacgatata cgacaaatga tggttatgtc tttaatccag ctgatattat
      661 cggagatacg ggtaatgctt atatcgttcc tcatggaggt cactatcact acattcccaa
      721 aagcgattta tctgctagtg aattagcagc agctaaagca catctggctg gaaaaaatat
      781 acaaccgagt cagttaagct attcttcaac agctagtgac aataacacgc aatctgtagc
      841 aaaaggatca actagcaagc cagcaaataa atctgaaaat ctccagagtc ttttgaagga
      901 actctatgat tcacctagcg cccaacgtta cagtgaatca gatggcctgg tctttgaccc
      961 tgctaagatt atcagtcgta caccaaatgg agttgcgatt ccgcatggcg accattacca
     1021 ctttattcct tacagcaagc tttctgcctt agaagaaaag attgccagaa tggtgcctat
     1081 cagtggaact ggttctacag tttctacaaa tgcaaaacct aatgaagtag tgtctagtct
     1141 aggcagtctt tcaagcaatc cttcttcttt aacgacaagt aagaaactct cttcagcatc
     1201 tgatggttat atttttaatc caaaagatat cgttgaagaa acggctacag cttatattgt
     1261 aagacatggt gatcatttcc attacattcc aaaatcaaat caaattgggc aaccgactct
     1321 tccaaacaat agtctagcaa caccttctcc atctcttcca atcaatccag gaacttcaca
     1381 tgagaaacat gaagaagatg gatacggatt tgatgctaat cgtattatcg ctgaagatga
     1441 atcaggtttt gtcatgagtc acggagacca caatcattat ttcttcaaga aggacttgac
     1501 agaagagcaa attaaggctg cgcaaaaaca tttagaggaa gttaaaacta gtcataatgg
     1561 attagattct ttgtcatctc atgaacagga ttatccaagt aatgccaaag aaatgaaaga
     1621 tttagataaa aaaatcgaag aaaaaattgc tggcattatg aaacaatatg gtgtcaaacg
     1681 tgaaagtatt gtcgtgaata aagaaaaaaa tgcgattatt tatccgcatg gagatcacca
     1741 tcatgcagat ccgattgatg aacataaacc ggttggaatt ggtcattctc acagtaacta
     1801 tgaactgttt aaacccgaag aaggagttgc taaaaaagaa gggaataaag tttatactgg
     1861 agaagaatta acgaatgttg ttaatttgtt aaaaaatagt acgtttaata atcaaaactt
     1921 tactctagcc aatggtcaaa aacgcgtttc ttttagtttt ccgcctgaat tggaaaaaaa
     1981 attaggtatc aatatgctag taaaattaat aacaccagat ggaaaagtat tggagaaagt
     2041 atctggtaaa gtatttggag aaggagtagg gaatattgca aactttgaat tagatcaacc
     2101 ttatttacca ggacaaacat ttaagtatac tatcgcttca aaagattatc cagaagtaag
     2161 ttatgatggt acatttacag ttccaacctc tttagcttac aaaatggcca gtcaaacgat
     2221 tttctatcct ttccatgcag gggatactta tttaagagtg aaccctcaat ttgcagtgcc
     2281 taaaggaact gatgctttag tcagagtgtt tgatgaattt catggaaatg cttatttaga
     2341 aaataactat aaagttggtg aaatcaaatt accgattccg aaattaaacc aaggaacaac
     2401 cagaacggcc ggaaataaaa ttcctgtaac cttcatggca aatgcttatt tggacaatca
     2461 atcgacttat attgtggaag tacctatctt ggaaaaagaa aatcaaactg ataaaccaag
     2521 tattctacca caatttaaaa ggaataaagc acaagaaaac tcaaaacttg atgaaaaggt
     2581 agaagaacca aagactagtg agaaggtaga aaaagaaaaa ctttctgaaa ctgggaatag
     2641 tactagtaat tcaacgttag aagaagttcc tacagtggat cctgtacaag aaaaagtagc
     2701 aaaatttgct gaaagttatg ggatgaagct agaaaatgtc ttgtttaata tggacggaac
     2761 aattgaatta tatttaccat cgggagaagt cattaaaaag aatatggcag attttacagg
     2821 agaagcacct caaggaaatg gtgaaaataa accatctgaa aatggaaaag tatctactgg
     2881 aacagttgag aaccaaccaa cagaaaataa accagcagat tctttaccag aggcaccaaa
     2941 cgaaaaacct gtaaaaccag aaaactcaac ggataatgga atgttgaatt cagaagggaa
     3001 tgtggggagt gaccctatgt tagatccagc attagaggaa gctccagcag tagatcctgt
     3061 acaagaaaaa ttagaaaaat ttacagctag ttacggatta ggcttagata gtgttatatt
     3121 caatatggat ggaacgattg aattaagatt gccaagtgga gaagtgataa aaaagaattt
     3181 atctgatctc atagcgtaag gaatagcagt agaaaaagtc tgaatcaaaa atgaagttct
     3241 ctcaaaagtt agaaataaaa ct
//
LOCUS       DAVGJL010000149         3239 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.150, whole genome shotgun sequence.
ACCESSION   DAVGJL010000149 DAVGJL010000000
VERSION     DAVGJL010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.150"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..246
                     /locus_tag="VVE42_001903"
     CDS             <1..246
                     /locus_tag="VVE42_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU3074044.1"
                     /translation="FLLPTLTTPSVIIMDNVRFHRMGKLELLCEEFGHKLLPLPPYLP
                     EYNPIEKTWAHIKKHLKRVLPSCNTFYEALLSCSCFN"
     gene            complement(453..2711)
                     /locus_tag="VVE42_001904"
     CDS             complement(453..2711)
                     /locus_tag="VVE42_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU3074045.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRPSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLKEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPIISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGATREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KEKLVDLGYDPKMGARPLRRTIQDYIEDTITDYYLENPSEKDLKAVMTSKGNIQIKSA
                     KKAEVKSSEKEK"
     gene            complement(2940..3170)
                     /locus_tag="VVE42_001905"
     CDS             complement(2940..3170)
                     /locus_tag="VVE42_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEU3074046.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
BASE COUNT          888 a          660 c          659 g         1032 t
ORIGIN      
        1 tttctcttac caacattaac cacaccatcg gttattatta tggataatgt aagattccat
       61 agaatgggga agctagaact tttatgcgaa gagtttgggc ataaactttt acctcttcct
      121 ccctacttac ctgagtacaa tcctattgag aaaacatggg ctcatatcaa aaagcacctc
      181 aaaagggtat taccaagttg caataccttt tacgaggctc ttttgtcctg ttcttgtttc
      241 aattgactat agcagattga aataagatga gaacaaatcg attgggaaag taaaattaat
      301 ttctataaat gttttagcaa ttgtttcgta ctatttcaga ttcagtctac tatatatcat
      361 aatggacaaa gggcaagctg tcaagaaatt acagttattt tagtaaaaaa agaagcagaa
      421 aaatttcatt ttctactcct tttctatagg atttattttt ctttttcaga acttttaact
      481 tcagcttttt tggcagattt aatctgaatg tttcccttgc tagtcataac tgctttgaga
      541 tctttttcgc ttggattttc aaggtagtag tcagtgattg tgtcctcaat atagtcttga
      601 atagtccgac gaagtgggcg tgctcccatt tttggatcat aacctaggtc aaccaacttt
      661 tccttgacct tgtcagttac atccaaacga atgttgttgc tagagaggcg cttgttaaca
      721 tctgctagca tgagctcgac aatctgaagg aggttatcct tgctgagagc cttaaattcg
      781 ataatgccat caaaacggtt cataaactct gggctaaaga agttaccgag ttcaccgagg
      841 acagaattgg tacgtccttc tctagtagca ccaaatccaa cgctagcttc ggtctttcct
      901 gtacctgcat ttgaggtcat gataatgatg gcatccttga agctaacggt gcgtccttgc
      961 ccgtctgtca aacgaccatc gtccaagact tgaagaaaca tgtgcataac atctgggtga
     1021 gctttttcca cttcatcgag aaggatgaga gaatatggat tgtggcgaac tttttcagtt
     1081 aattgaccag cctcatcata gccaacataa cctggagggg cgccgaccaa cttagccaca
     1141 ctatgttttt ccatgtattc actcatatca aagcgaatca tactatcagc agaaccaaaa
     1201 agttcgatag ccagttgttt ggaaagttct gtcttaccga caccagttgg cccaacgaag
     1261 aggaagcttc cgattgggcg gttaggggta ccaagtccga cacgattacg gcgaatagcc
     1321 ttggcaatct tatcgactgc atcatcttgg ccaataacat gagacttgag atcttcggct
     1381 agatggatga gttgagattg ttctttctct ttcaaatcac caacagggat attggttttc
     1441 tgctcgataa tgtgctcaat agttttctcg ctgatgatag gagtatcctg gtctgtgatc
     1501 tttttctttt gcatttcctt atacttggca atctggtcgc ggaagtaggc cgccttctca
     1561 aaatcttctt ctcgtgtagc ttgagacttg agattttcag cctcaatcaa gcgctgatca
     1621 attactttag gatccacaaa attcaaggtc aagttcatct tagaaccagc ttcatctagg
     1681 aggtcaatgg ccttgtcagg caagaagcga tcttggatgt agcgattgga aagagttgca
     1741 gctgcttcaa tcgcagcatc tgtatattga acgtggtggt aatcttcgta tttcttttga
     1801 atccctttga gaatagtgat tgtttcgtcc accgttggtt catcgacttt aacaggctgc
     1861 atacgacgct cgagggcagc atccttttca atgatacggt attcattgag ggtagtagca
     1921 ccgactagtt gcagttctcc acgagcaagg gctggcttga ggatatttcc tgcgtccata
     1981 ttaccatcac tcgcagaacc agcaccaaca atttcatgga tttcatcgat aaagaggatg
     2041 atgtcttcac gtttgcgaat ttcttccatg agtttttgca tgcgttcttc aaattgtcct
     2101 cgaatccccg ttccttgaac taagctaacc acatccagac ggatgacttg tttaccttgg
     2161 agtttatgtg gcacatcgcc atcgacaatt ttctgagcta gaccttcgac aacggccgtt
     2221 tttccgacac caggttcacc gataaggaca ggattattct tggttctacg attgagaatc
     2281 tcgatgacac ggataatctc atcgtcgcgc ccaataacgg ggtcaatgtc tccacgacgg
     2341 gcaatttcag ttacattaat accaaattct ttcagcaggc ctttttcttg gctaggtggc
     2401 ggagtttgag cagatccacg attttgggaa ccataaccgc cgtttccacc gtaacctcca
     2461 cctgattggg ttgggggaat aggaggagta ttgctagaag gtctgaaatt gtttagatca
     2521 ttgaagaaat caccaaaggg atcgaagtca cgattgttca gatccgtcat acctttgaag
     2581 aggctattgt taggatctgt tttgataatc ttatagcagt tttgacagag gtcaatttgt
     2641 ttttgttttc cattgagatt ggtgtaaaga tgaattgttg agtcgttaat tttacagttt
     2701 tgacaaagca tacatctacc tcatttcttt ctttagcctg acttgtttaa aggtcaaaaa
     2761 tagtcaaatt ctatactctc attataacag gattggggtg taaagcaagt aaaaagattg
     2821 aagctttgag aagttgttac aatgaaaatt tttgtgaatt tggactataa aaaaatccta
     2881 tttgtgagac aaaaaggatt ttacttagac atacagggac aatcccagct tgttttagat
     2941 tagtagagaa gttcgtaaat ttccattgcg attaagtcaa tgctatcaaa cgtatacgtt
     3001 tcgcgatctt ctacgtcacg aagggtaaag attgggccgt tttcttcgtc gtggctgtac
     3061 gcaacttctg caacaacaac tccttcgcgt tcaaaattac gttttaaatt tccgccatcg
     3121 tttgccattg cttcaaggcg gttgatgatt ctaaccaaat gtgattccat tttgattctc
     3181 cttcatttct tatcgttact attttaccat aaaggaggcc aacttgacta gaaaaaact
//
LOCUS       DAVGJL010000150         3179 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.151, whole genome shotgun sequence.
ACCESSION   DAVGJL010000150 DAVGJL010000000
VERSION     DAVGJL010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.151"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..582
                     /locus_tag="VVE42_001906"
     CDS             <1..582
                     /locus_tag="VVE42_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU3074047.1"
                     /translation="NKIFESIDNLFDIIDGDRGKNYPKSDELFSEEYCLFLNTKNVTK
                     NGFSFDTKQFITKTKDKLLRKGKLERYDIVLTTRGTVGNVAYYDELIKYKHLRINSGM
                     VILRPKTPNLNQKFIIHVLRNNNYSRVISGSAQPQLPITKLKKILLPLPPLALQNEFA
                     DFVALVDKSQLAIQKSLEELETLKKSLMQEYFG"
     gene            642..878
                     /locus_tag="VVE42_001907"
     CDS             642..878
                     /locus_tag="VVE42_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEU3074048.1"
                     /translation="MVVKTRKQGNSITITIPSEFNIPSGVKYEAKLLPSGEIIFTPEE
                     LGQQVSYVSDDAFDLNLDKIFDEYDDVFKALVEK"
     gene            875..1288
                     /locus_tag="VVE42_001908"
     CDS             875..1288
                     /locus_tag="VVE42_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEU3074049.1"
                     /translation="MTIYLTEKQIEKINALAIQRYSPNEKIQTVSSSALNMIVNLPEQ
                     FVFGKSLYPTIFDKATILFVQLIKKHVFANANKRTAFFVLVKFLQLNGYRFSVTVEEA
                     VKMCVTIAVEALTDEKMTSYSKWVSEHSVREKVKK"
     gene            1406..2371
                     /locus_tag="VVE42_001909"
     CDS             1406..2371
                     /locus_tag="VVE42_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU3074050.1"
                     /translation="MESKVTIIMQEMLPLLNNEQLLALRESLEHHLVDGKKQQKYSNN
                     NLLQLFITAKQVEGCSSKTIRYYQRTIENLFNAIKESVTQLTTDDLRSYLANYQSEKD
                     CSKANLDNIRRILSSFFAWLEQEEYIIKNPIRRIKKIKTEQNVKETYTDEHLEIMRDN
                     CENLRDLAIIDLLASTGMRVGELVQLNRSDIDFENRECVVFGKGKKERPVYFDARTKI
                     HLRNYLNDRKDSHPALFVTLVGKAQRLGIAGVEIRLRKLGDKLGIQKVHPHKFRRTLA
                     TKAIDKGMPIEQVQKLLGHSKIDTTLAYAMVNQNNVKHSHQKFIS"
     gene            2405..>3179
                     /locus_tag="VVE42_001910"
     CDS             2405..>3179
                     /locus_tag="VVE42_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU3074051.1"
                     /translation="MKKVKLGQVATFINGYAFKPQDWSSEGKEIIRIQNLTKTSKGIN
                     YYSGTIDKKYIVEAGDILISWSGTLGVFQWCGRSAVLNQHIFKVVFDKIDIDKSYFKY
                     VVEKGLQDAVKHTHGSTMKHLTKKYFDNIIVPYTNLGEQQRIASELDLLSKLILRRQE
                     QLEELNLLVKSRFNEMFGDPLNNNKKFAVKTGQQCFKFSSGKFLDKHDRVFEGYPAYG
                     GNGIAWKSRKYLIDNPTIIIGRVGAYCGNVRTTHGKVWIS"
BASE COUNT         1158 a          456 c          618 g          947 t
ORIGIN      
        1 aataaaatat ttgaaagcat tgataactta tttgatatta tagatggtga taggggcaaa
       61 aattatccta aatcagatga gttgtttagt gaggagtact gtttattttt aaatacaaag
      121 aatgttacta aaaacggatt ttcattcgat acaaagcaat ttatcactaa aacaaaggat
      181 aaattacttc gaaaaggcaa acttgagcgt tatgatatag tcttgacaac aagaggtact
      241 gttggaaatg tagcgtacta cgatgaatta ataaaatata aacatttacg tataaattca
      301 ggtatggtaa tattacgtcc caagacacca aatctaaatc agaaatttat tatccatgtt
      361 ttaaggaata ataattatag tcgagtgata tcaggaagtg ctcagcctca gttaccaatt
      421 acaaaattaa aaaaaatact tctccccctc cccccactag ccctccaaaa tgagttcgca
      481 gactttgtag ccctggtcga caaatcacaa ttggcaatcc aaaaatctct ggaagaactt
      541 gaaactttga agaaatctct gatgcaggag tattttggct gatattctgc cattgtaatt
      601 acggtaatga tttgttataa tacttcaaag gaggaaatca gatggtagta aaaacaagaa
      661 aacaaggaaa ttcaatcacc attacgattc caagtgaatt taatattcca agtggtgtta
      721 aatacgaagc gaaattgtta ccaagtggtg agattatctt tactcctgaa gaattggggc
      781 agcaggtttc ttatgtatct gatgatgcct ttgacttaaa tttagataaa atatttgacg
      841 aatacgacga tgttttcaaa gctttggtgg aaaaatgaca atctatctga cagaaaagca
      901 aattgaaaaa ataaatgctt tagcaattca acggtattct ccaaatgaga aaattcaaac
      961 agttagttct tctgccttaa atatgattgt gaacttacca gaacaatttg tctttgggaa
     1021 gtctctttat ccaacaattt ttgataaagc aacgatacta tttgtccaat tgataaagaa
     1081 gcatgttttt gctaatgcta ataaaagaac tgctttcttc gttttggtca aatttttaca
     1141 attaaacggc tatcgttttt ctgtaacggt agaagaagca gtaaaaatgt gtgtaaccat
     1201 cgcagtagaa gctttaactg atgaaaaaat gacaagctac tccaaatggg tttctgaaca
     1261 ttctgttaga gaaaaggtca aaaagtaacc tagtatgctg gatttgaatg agcacaagaa
     1321 aataaatgaa cagacaatat tagaattctg taatgcagaa actgatattg tctcttttta
     1381 ttgatgaata agaaagtgag aaattatgga atcaaaagtt acaattatca tgcaagaaat
     1441 gttacctctt ttaaataatg aacaattact agcgttgaga gaaagtttag aacatcatct
     1501 agtagacgga aaaaagcagc agaagtattc gaataataac ctgttgcaac tatttattac
     1561 cgccaagcag gtagagggct gtagctcaaa aacaattcgt tattaccaga ggacgattga
     1621 aaacttgttt aatgctatta aagagtctgt gacacaactc acaacagatg atttaaggag
     1681 ttatttagca aattaccagt ctgaaaagga ttgtagtaag gcaaatttag acaatattag
     1741 gcgtatattg tcttcttttt ttgcttggct tgagcaagag gaatatatca ttaaaaatcc
     1801 cattcgacgg ataaagaaaa ttaagactga gcaaaatgtg aaggaaactt atactgatga
     1861 acatttggaa attatgcgtg ataactgtga aaatttgaga gatttggcaa taatagacct
     1921 actagcatcg acaggtatgc gtgtagggga gcttgtacag ttgaatcgtt cagatattga
     1981 ttttgaaaac agagagtgtg ttgtctttgg taaaggaaag aaggagagac cagtatattt
     2041 tgacgctcgg acgaaaattc atttaagaaa ttatcttaac gacagaaaag atagtcaccc
     2101 tgctcttttt gtaacgctag ttggaaaagc ccagagactt ggaattgctg gtgtagagat
     2161 tcgcttaaga aagttaggag acaaactcgg catacaaaag gttcacccac ataagttcag
     2221 aagaacttta gcgactaagg caattgataa aggtatgcct atcgaacaag tccaaaaact
     2281 gctaggtcat agcaagattg acacaaccct ggcctatgcc atggtcaatc aaaataatgt
     2341 caagcattca caccaaaaat tcatctctta aaagcaaatc ccgatattcc gaggaggtgg
     2401 ttgcatgaag aaagtgaaat tagggcaagt ggctactttt atcaatggct atgcttttaa
     2461 acctcaagat tggtcatctg aagggaaaga gattatcaga atacagaatc tgactaaaac
     2521 ttcaaaaggg ataaattatt attcgggtac gattgataaa aaatacattg ttgaagctgg
     2581 tgatattctt atttcgtggt cgggtacatt aggggttttt cagtggtgtg gaagatccgc
     2641 agtgttaaat cagcacattt ttaaagtggt ttttgataag atagatatag ataaatcgta
     2701 ttttaaatat gttgttgaaa aaggtttaca agatgctgta aaacatactc atggttccac
     2761 aatgaaacat ttaactaaaa aatattttga taatattata gttccatata caaatttggg
     2821 agagcaacaa agaattgcaa gtgagctgga tttgttaagc aagctgattt taaggagaca
     2881 agaacaactt gaagagctta atctacttgt aaaatcccga tttaacgaga tgtttgggga
     2941 tcctttaaat aataacaaga aatttgcagt aaaaacggga caacagtgtt ttaaattttc
     3001 aagtggaaaa tttcttgata aacatgatag agtttttgaa ggttatcctg cctatggtgg
     3061 aaatggcatt gcttggaaaa gtcgaaaata tttaattgat aatcctacta tcatcattgg
     3121 tcgtgtaggt gcatattgtg gtaatgtaag aactacccac ggtaaggtat ggataagtg
//
LOCUS       DAVGJL010000151         3164 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.152, whole genome shotgun sequence.
ACCESSION   DAVGJL010000151 DAVGJL010000000
VERSION     DAVGJL010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.152"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            292..1578
                     /locus_tag="VVE42_001911"
     CDS             292..1578
                     /locus_tag="VVE42_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEU3074052.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFAAKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     ALIMIAAIEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMEELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYDLYDMPFGKERKSL
                     QGEIPEEFSMSAVDMSMIDHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     LESPEKFEAIKQDLVDEMWKVAQRELATGFYYGIPSENEQLFGARRKIPEYKFVAEVV
                     SYDDAVQTATIRQRNVINEGDQVEFYGPGFRHFETYIEDLHDAKGNKIDRAPNPMELL
                     TIKVPQPVQSGDMVRALKEGLINLYKEDGTSVTVRA"
     gene            1593..1904
                     /locus_tag="VVE42_001912"
     CDS             1593..1904
                     /locus_tag="VVE42_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3074053.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYDCKKYYACYRCHDS
                     LENHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCPFCFSRFNPGCQNHKERYF
                     K"
     gene            2164..2892
                     /locus_tag="VVE42_001913"
                     /pseudo
     CDS             2164..2892
                     /locus_tag="VVE42_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
BASE COUNT          981 a          547 c          684 g          952 t
ORIGIN      
        1 tagactgtat caatcctttt ccattatagg agcaacagag tgggagtagt catctaagga
       61 ctaatttata tatttttacg agtcagtatc ttgggatact ggtttttact tttttagact
      121 ttttgactac ttgttaaaac tggggtaatt ttcgactgtt taatagttat tatgcaaagt
      181 ctaaaagatt agaattgtca aaacaatccg tctaggcttg attttatcct ttatttacta
      241 taaaatgaga aggaaaaatg tcaaactttt atattgcaaa taggagaaat catgacaaaa
      301 acattaaaac gtcctgaggt tttatcacct gcagggactt tagagaagct aaaggtagct
      361 gttcagtatg gagcagatgc tgtctttatc ggtggtcagg cctatggtct tcgtagccgt
      421 gcgggaaact ttactttcga acagatggaa gaaggcgtgc agtttgcggc caagtatggt
      481 gccaaggtct atgtagcggc taatatggtt atgcacgaag gaaatgaagc tggtgctggt
      541 gagtggttcc gtaaactgcg tgatatcggg attgcagcag ttatcgtatc tgacccagcc
      601 ttgattatga ttgcagcgat tgaagcacca ggccttgaaa tccacctttc tacccaagcc
      661 agtgccacta actatgaaac ccttgagttc tggaaagagc taggcttgac tcgtgtcgtt
      721 ttagcgcgtg aggtttcaat ggaagaatta gctgagatcc gcaaacgtac agatgttgaa
      781 attgaagcct ttgtccatgg agctatgtgt atttcatact ctggacgttg tactctttca
      841 aaccacatga gtatgcgtga tgccaaccgt ggtggatgtt ctcagtcatg ccgttggaaa
      901 tacgaccttt acgatatgcc atttgggaaa gaacgtaaga gtttgcaggg tgagattcca
      961 gaagaatttt caatgtcagc cgttgacatg tctatgattg accacattcc agatatgatt
     1021 gaaaatggtg tggacagtct aaaaatcgaa ggacgtatga agtctattca ctacgtatca
     1081 acagtaacca actgctacaa ggcggctgtg gatgcctatc ttgaaagtcc tgaaaagttt
     1141 gaagctatca aacaagactt ggtggacgag atgtggaagg ttgcccaacg tgaattggca
     1201 acaggtttct actacggtat accatctgaa aatgagcagt tgtttggtgc tcgccgtaaa
     1261 attcctgagt acaagtttgt cgctgaagtg gtttcttatg atgatgcggt acaaacagca
     1321 actattcgtc aacgaaacgt cattaacgaa ggggaccaag ttgagtttta tggtccaggt
     1381 ttccgtcatt ttgaaaccta tattgaagat ttgcatgatg ccaaaggaaa taaaatcgac
     1441 cgcgctccaa atccaatgga actattgact attaaagtcc cacaacccgt tcaatcagga
     1501 gacatggttc gtgcattaaa agaaggactc atcaatcttt ataaggaaga tggaaccagc
     1561 gtcacagttc gtgcttaaga aaggaaaagg aaatgataga ggcacagggt ttcttagtgg
     1621 ataagcaaac aagatgcatt cattaccata gcaagctgga tattattgct ttacaatgct
     1681 atgattgtaa aaagtattat gcttgttatc ggtgtcatga ttcattagaa aatcaccctt
     1741 ttgagccgta tcccttatct ttgatacagg ataagcctat tttatgtggt gtttgtctaa
     1801 aactactaac atataagcaa tataaagaaa gcttaagttg ccccttttgt ttttctcgct
     1861 ttaatccagg ttgccaaaat cataaggaac gctattttaa atagcaaatc atctagtttt
     1921 gaagtaggag aaaactcaat tccaagagaa aatgaagtaa atcttcccac aataaaacgc
     1981 ataatatcag gattgttcaa tacctgatac tatgcgtttt taagatttta aagacttttt
     2041 tcctagcctc tttttacttt atctggtatt ttgactactt gttaaaactg gggtaatttt
     2101 cgactgttta acagttatta tgcaaagtct aaaaggttag aattgtcaaa acaatccgtc
     2161 tagagtatgc gtgatgccaa ccgtggtgga tgttctcagt catgccgttg gaaatacgac
     2221 ctttacgata tgccatttgg gaaagaacgt aagagtttgc agggtgagat tccagaagaa
     2281 ttttcaatgt cagccgttga catgtctatg attgaccaca ttccagatat gattgaaaat
     2341 ggtgtggaca gtctaaaaat cgaaggacgt atgaagtcta ttcactacgt atcaacagta
     2401 accaactgct acaaggcggc tgtggatgcc tatcttgaaa gttctgaaaa gtttgaagct
     2461 atcaaacaag acttggtgga cgagatgtgg aaggttgccc aacgtgaact ggttacagga
     2521 ttttactatg gtacaccatc tgaaaatgag cagttgtttg gtgctcgtcg taaaattcct
     2581 gagtacaagt ttgtcgctga agtggtttct tatgatgatg cgacacaaac agcaaccatt
     2641 cgtcaacgga acgtcattaa cgaaggggac caagttgagt tttatggtcc aggtttccgt
     2701 cattttgaaa cctatattga agatttgcat gatgccaaag gaaataaaat cgaccgcgct
     2761 ccaaatccaa tggaactatt gactattaaa gtcccacaac ctgttcaagc tggagacatg
     2821 gttcgagctc ttaaagaggg gcttatcaat ctttataagg aagatggaac cagcgtcaca
     2881 gttcgtgctt aatgtagttg tttagtttta aaaaattatg caaagctcca tatacaacac
     2941 ttaaacgaga ttaaagaatg gcgaaatccc ctgatgcgca agagattagc tgtctttttt
     3001 attttttaag tgataaagtc ggagcttagg tatcaaagcc tatcaaatta aacaaagaag
     3061 cgatgtctta gatattttga aaaaaattaa taagcagaaa actctctatt attttgttgt
     3121 agagagtttt ttgttaataa aatttcacaa aatgacattt atat
//
LOCUS       DAVGJL010000152         3009 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.153, whole genome shotgun sequence.
ACCESSION   DAVGJL010000152 DAVGJL010000000
VERSION     DAVGJL010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.153"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            108..2759
                     /gene="adhE"
                     /locus_tag="VVE42_001914"
                     /gene_synonym="adhC"
     CDS             108..2759
                     /gene="adhE"
                     /locus_tag="VVE42_001914"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEU3074054.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHA
                     ARIVRDAAIAAGAPENCVQWITQPSMEATSALMNHEGVATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKARQMVEFNGLGHSAAIH
                     TADEELTKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTIHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRELAFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
BASE COUNT          956 a          618 c          623 g          812 t
ORIGIN      
        1 ttgtgaaatg tttatgaaac tgtttacaaa gttgaaataa tgcgttataa taaacttgtg
       61 aaaaaattaa caaaggatat cgttccttga aagctatgga ggaaaatatg gctgataaaa
      121 aaactgtgac accagaggaa aagaaactcg ttgctgaaaa acacgtagat gagttggttc
      181 aaaaagctct agttgccctt gaagaaatgc gtaaattgga tcaagaacaa gttgactaca
      241 tcgttgccaa agcatcagta gcagctttgg atgcccacgg agaattggct ttacatgcct
      301 ttgaagaaac aggacgtggt gtatttgaag acaaagcaac taagaacttg tttgcctgtg
      361 aacacgtagt aaacaacatg cgccacacta agacagttgg cgttatcgaa gaagacgatg
      421 taacaggatt gactcttatt gctgaaccag ttggtgttgt ttgtggtatt actccaacaa
      481 caaacccaac atcaacagca atcttcaaat cattgatttc attgaagaca cgtaacccaa
      541 tcgtctttgc cttccatcca tcagcacaag aatcatctgc tcatgcagct cgtatcgtcc
      601 gcgatgcagc tatcgcagct ggtgctcctg aaaactgtgt gcaatggatt actcaaccat
      661 ctatggaagc aacaagtgcc cttatgaacc acgaaggtgt tgcgacaatc cttgcaacag
      721 gtggtaatgc catggttaag gcggcttatt catgtggtaa accagctctt ggggtaggtg
      781 ccggaaacgt tccagcttat gttgaaaaat cagcaaacat tcgtcaagca gcacacgata
      841 tcgtcatgtc taaatcattt gataacggta tggtctgtgc atctgaacaa gcagttatca
      901 ttgataaaga aatttacgat gaatttgtag cagagttcaa atcttaccac acttactttg
      961 taaacaaaaa agaaaaagct cttcttgaag agttctgctt cggcgtcaaa gcaaacagca
     1021 aaaactgtgc tggtgcaaaa ttgaacgctg acatcgttgg taaaccagca acttggattg
     1081 cagaacaagc aggatttaca gttccagaag gaacaaacat tcttgctgca gaatgtaaag
     1141 aagttggcga aaatgagcca ttgactcgtg aaaaattgtc accagttatt gcagttttga
     1201 aatctgaaag ccgtgaagat ggtattacta aggctcgtca aatggttgaa tttaacggtc
     1261 ttggacactc agcagctatc cacacagctg acgaagaatt gactaaagaa tttggtaaag
     1321 ctgttaaagc tattcgtgtt atctgtaact caccttctac ttttggtggt atcggggacg
     1381 tttacaatgc cttcttgcca tcattgacac ttggatgtgg ttcttacgga cgcaactcag
     1441 ttggggataa cgttagtgcc attaacctct tgaatatcaa aaaagtcgga agacggagaa
     1501 ataacatgca atggatgaaa cttccttcaa aaacatactt tgaacgtgat tcaattcaat
     1561 accttcaaaa atgtcgtgac gttgaacgtg tcatgatcgt tactgaccat gccatggtag
     1621 agcttggttt ccttgatcgt atcatcgaac aactggacct tcgtcgcaat aaggttgttt
     1681 accaaatctt tgcggatgta gaaccggatc cagatatcac aactgtaaac cgtggtactg
     1741 agattatgcg tgccttcaaa ccagatacca tcatcgcact cggtggtggg tctccaatgg
     1801 atgctgccaa agtaatgtgg ctcttctacg agcaaccaga agtggacttc cgtgaccttg
     1861 tccaaaaatt catggatatc cgtaaacgtg ccttcaagtt cccattgctt ggtaagaaga
     1921 ctaaattcat cgcgattcca actacatctg gtacaggatc tgaagtaaca ccatttgccg
     1981 ttatctctga taaagcaaac aaccgtaaat acccaatcgc tgactactca ttgacaccaa
     2041 ctgtggcaat cgtagatcct gctttggtat tgacagttcc aggatttgtt gctgctgata
     2101 ctggtatgga cgtattgact cacgcgacag aagcatacgt atcacaaatg gctagtgact
     2161 acactgatgg tttagcactt caagccatta aattggtctt tgaaaatctc gaaagctcag
     2221 ttaagaatgc agacttccac tcacgtgaga aaatgcataa cgcttcaaca atcgctggta
     2281 tggcctttgc caatgccttc ctaggtattt ctcactcaat ggcccataag attggtgcgc
     2341 aattccacac aatccacggt cgtacaaatg ctatcttgct tccatacgtt atccgttaca
     2401 acggtacacg tccagctaag acagcaacat ggcctaagta caactactac cgtgcagatg
     2461 aaaaatacca agatatcgca cgcatgcttg gacttccagc ttctactcca gaagaagggg
     2521 ttgaatctta cgcaaaagct gtctacgaac tcggtgaacg tattgggatc caaatgaatt
     2581 ttagagacca aggaattgac gaaaaagaat ggaaagaaca ttctcgtgaa ttagccttcc
     2641 tggcttatga agaccaatgt tcaccagcta acccacgtct tccaatggta gaccatatgc
     2701 aagaaatcat cgaagatgca tactatggct acaaagaaag accaggacgc cgtaaataat
     2761 tgtttatcag tctagaagca agacaaaaac tcaatttgag ggaaagaacc agtaattttt
     2821 ctatgataaa aggcatccta tcaaggtttt tgaatacctg ataggatgcc tttttatgat
     2881 gttgaggcct ttttgccctt tttgaaaaac tagaatagaa acaaaatata tagtaggttg
     2941 aaactagaat agtacacatc tgcttctaaa acattgttag aattcgattt gactgtcctg
     3001 atcgatttg
//
LOCUS       DAVGJL010000153         2900 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.154, whole genome shotgun sequence.
ACCESSION   DAVGJL010000153 DAVGJL010000000
VERSION     DAVGJL010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.154"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     regulatory      100..188
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            403..1071
                     /locus_tag="VVE42_001915"
     CDS             403..1071
                     /locus_tag="VVE42_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HEU3074055.1"
                     /translation="METQDYAFQPGLTVGELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFFEADEDGYFQKAFKE
                     LKVAENDYLEVTLHPVTKAFQDLMYSAVASSDYAHLLVMLVIAEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFAEWVQFLVDELNRVGKNREDLTELQQRWNQAVALELAFFDI
                     GYDV"
     gene            1314..2096
                     /gene="thiM"
                     /locus_tag="VVE42_001916"
     CDS             1314..2096
                     /gene="thiM"
                     /locus_tag="VVE42_001916"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEU3074056.1"
                     /translation="MTSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPA
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPAVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVTIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVY
                     NIAGEIAEKHPNGHLPGTFKVEFINALYEITDEDVKEFKRVK"
     gene            2098..2727
                     /locus_tag="VVE42_001917"
     CDS             2098..2727
                     /locus_tag="VVE42_001917"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEU3074057.1"
                     /translation="MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKG
                     EGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIRNEEEFQQSKVEYVDYVGVGPVFGTQSKDDAGGTIGYEGLELMRKLL
                     PQMPLVAIGGIQTKHIKDIIKTNMDGVSIISAISYAKNIEKTVREMSEQ"
BASE COUNT          969 a          417 c          621 g          893 t
ORIGIN      
        1 actattccag tttcaatata ttatatataa agatattttt cggtcaaata tcttgctagg
       61 gctttcattt tttgctatac tggttcagta attaaataat agagacattt ggggtgcttt
      121 aggctgagat aatacccatt gaacctgata cagttaagac tggcgaaggg aaatgtgaac
      181 agttttttcg tatggtgaaa tgaacggtct aatcttgtaa gtcaactcta attcttgatt
      241 gtaattggag ttttttgttt atataaactg gataggcttg tttagttagc tctctgcaaa
      301 tgctctcgat gtgctttaaa aggatcaagt ggaattacga ttttgcttga tatttcttgg
      361 tgttttatta attactaaca agtaaagaga ggagaagtaa atatggaaac acaagactat
      421 gcatttcagc caggtttgac tgttggagaa ttattaaaaa gcagtcagaa ggattggcag
      481 gctgcaatca atcatcgttt tgttaaggaa ctttttgcgg ggacaattga gaataaggtc
      541 ttaaaggact atctgattca agattatcac ttctttgatg ccttcttatc catgctgggt
      601 gcctgcgtag ctcacgcaga taaacttgaa tccaagcttc gttttgccaa gcaactaggc
      661 ttttttgaag cagatgaaga tggttatttc caaaaggctt ttaaagagtt aaaagtagca
      721 gagaatgact atctagaagt gactttgcac cctgtaacaa aagcctttca ggatctaatg
      781 tattcggcgg tggcttcatc agactatgcc catcttttgg tcatgctggt cattgcagaa
      841 ggtctctatt tagactgggg ttctaaagat ttggctttac ctgaagtcta tattcattcg
      901 gaatggatca atctccacag aggtcctttc tttgcagagt gggttcaatt tctggttgac
      961 gaactcaatc gtgtcggtaa aaatcgagaa gatttgacag aacttcagca acgctggaat
     1021 caagcagtcg ctttagaatt agcttttttt gatattggtt acgatgtcta gagaaggctc
     1081 atgatattta caaatttgat gaggtaagac tcttcgaaaa tctcttcaaa ccacgtcagt
     1141 tctatccaca acctcaaaac agtgttttga gcaacctgcg gttagcctcc tagtttgctc
     1201 tttgattttc attgagtata aatcattcaa tgtaaatgaa aaaaattctt aacgaaagat
     1261 agaaataaat cgaaatcagt agatcgtata aagtgaaaag gaaggatttc aatatgacaa
     1321 gtttaaaatt attaaaagaa aaagcaccat tggtcatttg tataaccaat gatgtagtaa
     1381 aaaatttcac agcaaatgga ttagtagcac tgggcgcctc gccagccatg agtgagtttc
     1441 cagcagattt agaggatttg ttaaagtatg ctggtggttt attaataaac ataggaacat
     1501 tgacagatga aaattggaaa ttataccaag ctgctctgaa aattgcggag aaatataatg
     1561 tcccagcagt tttagatcct gtagcctgtg gagcaggaga atatagaaaa aaagtagcag
     1621 atgatctaat caacaattat aaactagcag cgattagagg aaatgctggc gagattgcct
     1681 ctttagtagg aatagatgtg gcatctaaag gagtagatag tgcgggcgta gataatattg
     1741 acgaaattgc tctagcagca aatgagaagt tcaatattcc aatagtagta acaggtgaag
     1801 tggatgccat tgctgttaat ggagaagtgg taacgattca taatggtagt gctatgatgc
     1861 cgaaagtcat tgggacagga tgcttattag gagctgtagt agcaagcttt atcggactag
     1921 aaaaaggtca agaattgaaa tcattagaaa cagcaatgtt ggtttacaat atcgctggag
     1981 aaatagcaga aaaacatcca aatggacatc ttcctgggac atttaaagtt gaatttataa
     2041 atgccttata cgagattaca gatgaagatg taaaggaatt caaaagagtg aagtaagatg
     2101 tttcataaag aattactaaa actatatttt atttgtggaa cgactacttg ccaaggaaaa
     2161 aatctatata cagtcgttga ggaagcctta aaaggtggta taaccttatt tcaatttcga
     2221 gaaaaaggtg agggtgcttt agaaggctta gaaaaacttg agctagctat ccaaattaaa
     2281 gaactttgta aaaaatacaa tgttccattt atcgttaatg acgatataga tttagcaatg
     2341 gaaattgacg ccgatggcgt acatgtaggt caagatgata ttggtgttga tgaaattaga
     2401 aaattgatgc cagataaaat aattggtctt tctataagaa acgaagaaga atttcaacaa
     2461 tcaaaagttg aatatgtaga ttatgtaggt gttgggcctg tatttggtac ccagtcaaag
     2521 gatgatgctg gtggtactat aggttatgaa ggtcttgaat tgatgagaaa actattgcca
     2581 caaatgcctt tagttgcaat tggtgggata cagacgaaac atattaaaga cattataaag
     2641 accaatatgg atggcgtttc aatcatttcg gcaatatcgt atgcaaaaaa tatagaaaaa
     2701 actgttcggg aaatgagtga acaatagata tctatccttg gattctaagt aagtaggatt
     2761 ttcctttttt aaaaagctat gtccagattc agcttgaatg gctaattcat caaacaggtc
     2821 atttgttttt tgcaaaagaa aaatctgctg tctttgtgag tgcaaggctg tatttccagt
     2881 tcaatttctt gaactagtat
//
LOCUS       DAVGJL010000154         2886 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.155, whole genome shotgun sequence.
ACCESSION   DAVGJL010000154 DAVGJL010000000
VERSION     DAVGJL010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.155"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(97..630)
                     /locus_tag="VVE42_001918"
     CDS             complement(97..630)
                     /locus_tag="VVE42_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEU3074058.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
     gene            complement(719..1495)
                     /gene="recX"
                     /locus_tag="VVE42_001919"
     CDS             complement(719..1495)
                     /gene="recX"
                     /locus_tag="VVE42_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEU3074059.1"
                     /translation="MKITKLEKKKRLYLMELDNGDKCYITEDTIVRFMLSRDKVISEE
                     ELKEIQDFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDIDKNIVSQVIANLKEDKW
                     INDGQYAYAIINTNQLSGDKGPYVLTQKLAQKGISKSTIEENLKEFDFSEVAQRVANK
                     LLKKYEGKLPARALQDKIIQNLTNKGFSYSDAKIAFDDLDSQVDQETTQELIFKELDK
                     QYTKYARKYEGYELKQRLTQVLARKGYDFSDIASALREYL"
     gene            1533..>2886
                     /gene="rlmD"
                     /locus_tag="VVE42_001920"
     CDS             1533..>2886
                     /gene="rlmD"
                     /locus_tag="VVE42_001920"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEU3074060.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQ
                     ALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
                     DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNR
                     QLNLTQLVKELVKDFPEVVTVAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSL
                     SPRAFYQLNPEQTEVLYSEAVKALDVDKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDI
                     IPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLL
                     DTILTYVPEKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKK
                     V"
BASE COUNT          910 a          525 c          528 g          923 t
ORIGIN      
        1 ctgctataat ggatttattc ctttttgtgt ttacacaatt tattttatac taccaaaaaa
       61 acaaggtcag gattttgttc ctgacctttg acaactttac cgattcttta gttctacata
      121 gcgcttgtac caaatgttta cataggcttc tgagaaagga ccacgtccat tgttaatcca
      181 atcaacaaga attttgacat gttcttttaa aatatagtcc aagtcatcag aataattcat
      241 tttgcgtttg tgacgctcgt actcttcaac gtccaagaga cgtttttccc catctgtaaa
      301 aattttaaca tccaaatcgt aatcaatata cttcagtgct tcttcatcca gatagtaggg
      361 gctagccata ttgcaatagt aagaagttcc attatcacga atcatggcaa tgatattaaa
      421 ccaatatttc ttgtgaaagt aaacaatagc cggttctcga gtgacccaac gacgaccatc
      481 actttcggta acaagtgtat gatcgttgac accaataatg gcgttttctg ttgtttttag
      541 taccatggtg tcccgccaag ttcggtggag actcccatca tgcttataac tttgaattgt
      601 aataaagtcg ccttcttttg gaagcttcat aactaaccaa ctttctacaa tttataagtt
      661 tatcatttac tattgtatca taaaattacc taaaatctgt gaatttcact tggaaatatt
      721 aaagatattc tctaagagcg cttgctatat ccgaaaaatc gtagcccttt cgtgctaaaa
      781 cttgagttaa acgctgcttc agttcgtatc cttcatactt tcgagcatac ttagtatatt
      841 gcttatcaag ttccttgaag atgagttctt gagtcgtttc ttggtcaact tggctatcta
      901 agtcgtcaaa ggcaatttta gcatcagaat aagagaagcc cttgttagtc aagttctgga
      961 taatcttatc ttgcaaggca cgagctggaa gttttccctc atattttttc aatagtttat
     1021 tagctacacg ttgagcaact tcagaaaaat caaattcttt caagttctct tctatagtag
     1081 attttgaaat ccctttttga gctagtttct gagtcagcac ataaggaccc ttgtctcctg
     1141 aaagttgatt ggtattgatg atagcataag cgtactgacc atcattaatc cacttatctt
     1201 ctttaagatt agcaataact tgagaaacga tgtttttatc aatatcgtat tttttcagat
     1261 attctctgac ttctttttca gtgcgtgctt taaaggataa gtggtagagg gccagattct
     1321 taccataaga aaattgagca aagtcttgaa tctctttcaa ttcctcttcg cttatcacct
     1381 tatctctcga taacataaaa cgaacaattg tatcttcggt gatatagcat ttgtcgccat
     1441 tatcaagctc catcagatag agtctttttt tcttttcaag ttttgtgatt ttcatagctc
     1501 tattataact caaaatgtga taagataggg gtatgaatct gaaagtgaaa caaaaaatac
     1561 cattaaaaat caagcgcatg ggaattaacg gtgagggaat cggcttttac caaaaaacat
     1621 tagtctttgt accaggagct ctcaaaggcg aagatatcta ttgtcagatt acttctatta
     1681 gacgcaactt tgttgaagca aaattactga aggtcaacaa gaagtctaaa tttcgaattg
     1741 tgccatcttg tactatttat aatgaatgcg gaggctgcca aatcatgcac ctgcattatg
     1801 ataagcagct ggagttcaag acggacttac ttcatcaagc gctgaaaaaa tttgctcctg
     1861 caggatatga aaattatgaa attcgtccaa ctattggaat gcaggaacca aaatattaca
     1921 gagctaagtt acaatttcag actcgaaaat ttaaaaatca ggtcaaggcg ggcttatatg
     1981 cacaaaactc tcactattta gtagagttga aagactgcct ggtacaagat aaggaaaccc
     2041 aagtgattgc taatcgctta gcagaattac ttacttatca ccagattcca atcacggatg
     2101 agagaaaagt tctaggtgtc cgtactatta tggtccgacg cgcgagaaag accggacagg
     2161 ttcagattat tattgttaca aaccgccagc ttaatttaac tcaattggta aaagagttgg
     2221 ttaaagattt cccagaagtt gtgacagtag ctgttaatac aaatacagct aaaaccagtg
     2281 agatatatgg tgaaaagaca gagattatct gggggcaaga gagtattcaa gaaggtgtac
     2341 tcaattatga attttcacta tcccctcgag ctttttatca actaaatcct gagcaaacag
     2401 aagtcctcta tagcgaagca gtaaaagcgc tggatgttga taaagaagac catttgattg
     2461 acgcttattg tggagttgga acgattggat ttgcctttgc aaagaaagta aaaacactca
     2521 gaggtatgga tattattcca gaagctattg aagatgccaa gcgaaatgct aaaagaatgg
     2581 gatttgacaa tactcattat gaagctggaa cggcagaaga gattattcct cgttggtaca
     2641 aggaaggcta ccgagcagat gctttgattg ttgacccacc acgtacaggt ctggatgata
     2701 agttattaga tactattctt acttatgtac cagaaaaaat ggtttatatt tcttgtaatg
     2761 tttcgacctt ggctcgtgat ttggtacgct tagtagaagt ctatgatctt cattatatcc
     2821 agtcggtcga tatgttccca catacagctc gaactgaagc tgttgtaaaa ttaataaaaa
     2881 aagttt
//
LOCUS       DAVGJL010000155         2800 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.156, whole genome shotgun sequence.
ACCESSION   DAVGJL010000155 DAVGJL010000000
VERSION     DAVGJL010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.156"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            355..831
                     /locus_tag="VVE42_001921"
     CDS             355..831
                     /locus_tag="VVE42_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU3074061.1"
                     /translation="MIGKNIKSLRKTHDLTQPEFARIIGISRNSLSRYENGTSSVSTE
                     LIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIEIVKERGANLLSRLYRYQDSQGI
                     SIDDESNSWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEISEKRMVA"
     gene            831..1601
                     /locus_tag="VVE42_001922"
     CDS             831..1601
                     /locus_tag="VVE42_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEU3074062.1"
                     /translation="MEIQDYTDSEFKHALARNFCSLIRGKKSSKQPIAILLRGQSGAG
                     KTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQEYGKDSVEYTKDFAGKMVESLV
                     TKLSSLRYNLLIEGTLRTVDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
                     LYIINPNQARATPKEHHDFIVNHLVDNTRQLEELAIFERIQIYQRDRSSVYDSGENTT
                     SAAEVLQEYLFGKWSKVEEEMLKSGEERLKDLTNRNGC"
     gene            complement(1955..2797)
                     /locus_tag="VVE42_001923"
     CDS             complement(1955..2797)
                     /locus_tag="VVE42_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU3074063.1"
                     /translation="MSSHTIRAYKNDLMQFYNSDYDNVESYIEQLTRSNIKTNTLRRK
                     IACMKVFYNYLKYQNIIEETPFNQLRFQFRTEKVLPKTIPYDILKSIFIYLEQKVIVS
                     KTDYQKQHAERNLLIISLLLSTGIRISELCHIHLKDINLSNKTLHIIGKGKKERILFL
                     GDQKTFNLLETYINKTRNESNDFLFPGKHSLKPLSEQSVRLVIKRIVEQNNFSRTITP
                     HMFRHSFATMLLDSDVDIRYIQQILGHSSISITQIYTHVSHSKQKEILSSFNPVSVIH
                     SEIE"
BASE COUNT          975 a          363 c          516 g          946 t
ORIGIN      
        1 acactttatt gaggtattgg atacgctttg ctaatagcct aataatcaca tgtggagtgt
       61 tgctacaacg aaaaaggtga taatccttga tttcaagcta ttttataagc attttgtctt
      121 tgtagataaa ggcaattttg acaataaaaa tcctaaaagg tgaatcgtta tagatgtatt
      181 tgtagatatc gtttgcgcat cgaaaaaatt aatacaagaa taaatattta tggctcttta
      241 ggtgactttt atagaagtaa agtttaggat agaaaaacaa gaaataatgc accatttttg
      301 gtgcgttatg cttttttatg ctataatgga attataaaaa taaaggagtt tgctatgatt
      361 ggaaagaaca taaaatcctt acgtaaaaca catgacttaa cacaacccga atttgcacga
      421 attataggaa tttctcgaaa tagcttgagt cgttatgaaa atggaacgag ttcagtctct
      481 acggaattaa ttgacatcat ttgtcagaag tttaatgtat cttatgtcga tattgtagga
      541 gaagataaaa tgctcaatcc tgttgaagat tatgaattga ctttgaaaat tgaaattgtg
      601 aaagaaagag gtgctaatct actttctcga ctctatcgct atcaagatag tcagggaatt
      661 agcattgatg atgaatctaa ttcttggatt ttaatgagtg atgatctatc tgatttgatt
      721 catacgaata tctatttagt agaaactttt gatgaaatag agagatatag tggctatttg
      781 gatggaattg aacgtatgtt agagatatct gaaaagcgga tggtagccta atggaaatcc
      841 aagattatac tgatagtgaa ttcaaacatg ctctagcacg gaacttttgc tcactgataa
      901 gaggaaaaaa atccagtaaa caacctatag cgattttgct tagagggcaa agtggtgctg
      961 gtaaaactac aattcatcgt ataaaacaga aagaatttca aggaaatatt gttatcatag
     1021 atggtgatag ttttcgttct cagcatccac actatttaga actgcagcaa gaatatggca
     1081 aagacagtgt agaatatacc aaagattttg caggaaaaat ggtagagtct ttagtaacaa
     1141 aattgagtag tttgagatac aatcttttga tcgagggaac tttacgaaca gttgatgttc
     1201 caaagaaaac agcacaactc ttgaaaaata agggatatga agtacaattg gccttaattg
     1261 cgacaaagcc tgaattgtcg tatctaagta ctcttatccg ttatgaagaa ctgtacatta
     1321 tcaatccaaa tcaagcacgc gcaactccaa aagaacatca tgatttcatt gtaaatcatc
     1381 tagttgataa tacacgacaa ttggaagaac tggctatctt tgaaagaatt caaatttatc
     1441 aacgagatag aagttctgta tatgattcag gagaaaatac aacttcagca gcagaagtgc
     1501 tacaagaata cctcttcgga aaatggagca aggtagagga agagatgctt aaatctggag
     1561 aagaaagatt gaaagattta actaatcgaa atggttgtta gaacacaaat tttaaaatat
     1621 aatctactta taacttttgt tagttgttta aggtgggcga aaatttttta gaaccaaaaa
     1681 acgcatagtc tcaggtgttg aaaaacttga aattatgcgt tttcttcagg aattgagatt
     1741 ttgcccatct ctttttatta tatttaagga tatttgaaag atgtatttta gaaaaacaat
     1801 tatggagttc ttaatacgga atatagtata aatgctgatt caaagtgaag ttttatatgt
     1861 tttttgcttt tgttgagtat aaaatctatt aactaaagtc gaactacaaa aattaattta
     1921 agatgaagtg aactgtagac tttggagatg tctcttactc gatttcagaa tgaattactg
     1981 atacgggatt aaaagaacta agtatttctt tttgttttga atgagatacg tgagtataga
     2041 tttgtgtgat tgatatagaa ctatgtccaa gaatttgttg aatatatcga atatctacat
     2101 cactatctag aagcattgtc gcaaagctat gtctaaacat atgcggtgta atagttctag
     2161 aaaagttatt ttgttcaacg attctcttta ttactaaacg tacactttgc tctgacaatg
     2221 gtttaagtga atgtttccct gggaacaaga aatcattgga ttcatttctt gttttattta
     2281 tatatgtttc taataaattg aatgtttttt gatctcctaa aaataggata cgttctttct
     2341 taccctttcc tataatatgg agtgtcttat tggaaagatt aatgtctttg agatgaatgt
     2401 ggcaaagttc agaaattctg atgcctgttg aaagtaaaag tgaaataatt agtagatttc
     2461 tttcagcgtg ttgtttttga tagtcagttt tagatacaat tactttctgt tctaaatata
     2521 taaaaatgct tttcagaatg tcatacggaa tcgttttagg caatactttt tcagttctaa
     2581 attgaaaacg caattgattg aagggggtct cttcaattat gttctggtat tttagatagt
     2641 tataaaacac cttcatacaa gcaatttttc ttcttaatgt attcgttttt atgttagatc
     2701 gagtcaactg ttctatatag gattcgacat tatcatagtc tgagttataa aattgcataa
     2761 gatcattctt ataagcacga atcgtgtgtg aactcaaacg
//
LOCUS       DAVGJL010000156         2703 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.157, whole genome shotgun sequence.
ACCESSION   DAVGJL010000156 DAVGJL010000000
VERSION     DAVGJL010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.157"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            43..1050
                     /locus_tag="VVE42_001924"
     CDS             43..1050
                     /locus_tag="VVE42_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU3074064.1"
                     /translation="MTEKLINSKPNGVFALILIELTIVLGIFIFIMGVGSENIFGIII
                     GPLLIVIAGLAHAGLKVVKPQEALVLTLFGNYTGTIKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNDNDVNLEIGKKHISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQSRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVEMALERLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            1053..1253
                     /locus_tag="VVE42_001925"
     CDS             1053..1253
                     /locus_tag="VVE42_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU3074065.1"
                     /translation="MAEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
     gene            1405..1587
                     /gene="rpmF"
                     /locus_tag="VVE42_001926"
     CDS             1405..1587
                     /gene="rpmF"
                     /locus_tag="VVE42_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEU3074066.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKIAKAASAE"
     gene            1603..1752
                     /gene="rpmG"
                     /locus_tag="VVE42_001927"
     CDS             1603..1752
                     /gene="rpmG"
                     /locus_tag="VVE42_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU3074067.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(2244..>2703)
                     /locus_tag="VVE42_001928"
     CDS             complement(2244..>2703)
                     /locus_tag="VVE42_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU3074068.1"
                     /translation="GLWYYLNESGSMATGWVKDKGLWYYLNESGSMATGWVKDKGLWY
                     YLNESGSMATGWVKDKGLWYYLNESGSMATGWVKDKGLWYYLNESGSMATGWVKDKGL
                     WYYLNESGSMATGWFTVSGKWYYTYNSGDLLVNTTTPDGYRVNANGEWVG"
BASE COUNT          885 a          539 c          481 g          798 t
ORIGIN      
        1 aagtgatatc atttagatat caaataatgg gaggtcattc ttatgactga aaaactaatc
       61 aattcaaaac caaatggtgt attcgcattg attctcattg agttgacaat cgtacttggt
      121 atctttatat ttataatggg ggttggttcg gaaaacattt ttggaattat tatcggacct
      181 ttactaatcg taattgcagg tctagctcat gctggtttaa aagttgtcaa acctcaggaa
      241 gctctggttc tgacactctt tggtaattat acaggtacca tcaaagaacc tggtttttac
      301 tttgtcaatc ccttcagcgt agcagtcaac cctgcaaacc acactcgact tggacaaagt
      361 ggtgatgtta gcacaaaatc tcctttttta ggagctaaat catcaaatga caatgatgta
      421 aatcttgaaa ttggcaagaa acacatttcc ctcaaagtca tgaccttgag caattctcgt
      481 caaaaaatca atgattgctt aggaaaccct gtagaaatcg gtatcgcggt aacttggaga
      541 gttgtcgata ccgctaaggc agtcttcaac gttgataact acaaagaata tctttcattg
      601 caatgtgata gcgccctccg taatattgtc cgcatctatc cttacgatgt gtctcctaat
      661 gtggatacta cgggtgacgg gcaagcagat gaaggtagtc tccgtggctc tagcgaaatt
      721 gttgctaacc gtattcgtga agaaatccaa agtcgtgttg aggatgctgg cttggaaatc
      781 cttgaagcac gtatcactta cctagcttat gctccagaaa ttgctgccgt tatgcttcaa
      841 cgccaacaag catctgccat tattgatgca cggaagatga ttgtagatgg tgctgtagga
      901 atggttgaaa tggcactaga acggctcaat gaaggggaat tggtagaact tgacgaagaa
      961 cgaaaagctg ccatggtttc aaatctcctt gtcgttcttt gtggcaatca tgatgcacaa
     1021 ccaattgtca acacaggaag tctttactaa agatggctga agctaaaaaa aagcagatcc
     1081 cccttcgact ctcaacaaag ttatacgctg cactcgcatc atgggcagaa gacgactttc
     1141 gttcagtcaa tgggcaaatc gaatatctcc tcacagagtg tgtcaagcaa cggaagaaag
     1201 acggaaaata cgtatcagaa accattgacg aaccatttga gattgatatt taacaccttc
     1261 tcctgtctgc cgtgtcggat aggagaattt tttcatcttt ttttgtcaag taactttact
     1321 ttacaaaaaa aatgtgttat cctagtatgg ttgatgaaaa tcagtagatt gaatcgaata
     1381 taaatatcta aaggagaaat caaaatggca gtacctgcac gtcgcacttc aaaagcgaag
     1441 aaaaacaaac gtcgtacaca ctacaaagta acagctccat ctgtaaactt tgacgaaact
     1501 actggagatt actcacgttc tcaccgtgta tcacttaaag gatactacaa aggacgtaaa
     1561 atcgctaaag ctgcatcagc tgaataatag aagggagata ccatgcgcgt aaatattaca
     1621 cttgaacaca aagaatctgg tgaacgcttg taccttactt ctaaaaacaa acgtaacact
     1681 ccagaccgcc ttcaattgaa gaaatactca ccaaaacttc gcaaacacgt tgtgtttaca
     1741 gaagttaagt aagcattcaa tacgaatcaa tacagaagaa aaacgcttga tcagcgtttt
     1801 ttctttttgt taatttcact atattgcaag ggaaatcatt cgaatgacta catttttcac
     1861 tacctttttt gaaataagaa tacggattcg gacgattaaa agtaagagaa actcaaatgt
     1921 aagttggggt tccttttttg tatatttttc aaataacgcc tctacactat ttgtagcaaa
     1981 ttcaccaact acagttgtat cttagttaaa ataagttaga atatgtaagt gagtaccaga
     2041 tataccaaga catcgtcacc atctaaggta tattcaaaat acaaaagttg accaactaga
     2101 tttctgaata tccttatata tccattctta aaattggttt aaatagcgta gtcttttaaa
     2161 ctagttttga gaatccaaaa aatcttccta catatgtaag aagatttttt agttcagaat
     2221 gattagattt agctaatgga tacctatcct acccactcac cgttagcatt gactcgatag
     2281 ccatcgggtg tagtcgtgtt tactaataaa tctcctgaat tataggtata gtaccattta
     2341 ccagaaactg taaaccaacc agtagccatt gaacctgatt cgtttaagta ataccataag
     2401 cctttgtctt taacccaacc agtagccatt gaacctgatt cgtttaagta ataccataag
     2461 cctttgtctt taacccaacc agtagccatt gaacctgatt catttaagta ataccataag
     2521 cctttgtctt taacccaacc agtagccatt gaacctgatt catttaagta ataccataag
     2581 cctttgtctt taacccaacc agtagccatt gaacctgatt cgtttaagta ataccataag
     2641 cctttgtctt taacccaacc agtagccatt gaacctgatt cgtttaagta ataccataaa
     2701 cct
//
LOCUS       DAVGJL010000157         2658 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.158, whole genome shotgun sequence.
ACCESSION   DAVGJL010000157 DAVGJL010000000
VERSION     DAVGJL010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.158"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..1670
                     /locus_tag="VVE42_001929"
     CDS             <1..1670
                     /locus_tag="VVE42_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEU3074069.1"
                     /translation="KIKVYLYTRVSTSIQIEGYSLEAQKSRMKAFAIYNDYEIVGEYE
                     DAGKSGKSIEGRIQFNRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIMQDYG
                     VNLICVEDGIDSSKDAGKLMISVLSAVAEIERENIRIQTMEGCIQKAREGKWNGGFAP
                     YGYKLEDGKLFINEEEAVAIRTIFDQYVNTTIGANGISKYLENHGIRKIPRQNGKNPL
                     FDAGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYKQVEQDEYLISEGIHEAIVSDE
                     VWQAAQVKLKSQAKKYEHVNKGKDTRTHLLSGIVKCPICGVGMFGNKCIKKKKDGTKY
                     KDFYYYGCKHRQMIRGHKCTFSKQIREELLDDAVAEVIVKIVSNPKFASMMQEKINMK
                     VDTSEIEKEIDNYQKELRKSHSTKFKLIEEIDNLDVEDKHYKRRKQDLDDRLYRMYDK
                     IDELESSLIDAKAKKQTIEAEKLTGDNIYKVLIYFDKLYKVMNDVERRQLISALISEI
                     QVYEEKQSNGQWLKSITFKLPIIEENLNIDLDNDEQVECVSLLEKRS"
     gene            1938..2504
                     /locus_tag="VVE42_001930"
     CDS             1938..2504
                     /locus_tag="VVE42_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3074070.1"
                     /translation="MVILELYQNDYSKDLVAFDSIEDGKAFVAQIPGYTLETEDGFEV
                     EYFNPKNIPDYMEIIFNGNIVPLSKFMFDPEENVNIIWKEISNLSLKNDRVIEGYSKI
                     DAYVVNNHEVKAYVETRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVW
                     HFLCHLDPMFVEIEDVEGYVKEEVGEIQ"
BASE COUNT          992 a          337 c          588 g          741 t
ORIGIN      
        1 aaaaaataaa agtatacctc tatacacgag tatctacgtc aatacagata gaggggtatt
       61 cgttagaggc acaaaaatca cgaatgaaag cttttgctat ttacaacgat tatgaaattg
      121 ttggagagta tgaagatgcc gggaagtctg gcaagtctat tgaaggtaga atacagttca
      181 atcgaatgat ggaagatata aaatctggaa aagatggagt atcttttgtt cttgtgttta
      241 agttatcaag atttgcaaga aatgccgctg atgttctttc tacacttcag ataatgcagg
      301 attatggagt taatcttatc tgcgttgaag atggcattga ttcatctaaa gatgctggga
      361 aactaatgat ttctgtttta tcagctgtgg ctgaaataga aagagaaaac atccgtattc
      421 aaacaatgga aggttgcatt caaaaagcaa gggaaggaaa atggaatggt ggctttgctc
      481 cgtatggata taaacttgaa gacggcaagc tgtttataaa tgaggaagag gcagttgcaa
      541 taagaacgat tttcgaccag tatgtaaata ctacgatagg agccaatggg atatctaaat
      601 acttagagaa tcatggaatt agaaaaatcc caagacagaa tggtaagaat cctttgtttg
      661 atgcaggtct tataagaaag atattaaaga atcctgtata taatgggaaa atagcatttg
      721 gaagaagaac tttagaaaaa gttcatggta caagaaatga atataagcag gttgaacaag
      781 atgaatatct aatatctgaa gggatacatg aagctatagt ttccgatgaa gtttggcaag
      841 ctgctcaggt taagctaaaa tctcaagcaa agaaatatga gcatgtgaat aaaggaaaag
      901 atacacgcac acacttgctt tctggaattg taaaatgccc gatatgtgga gtgggaatgt
      961 ttgggaacaa gtgtatcaag aaaaagaaag atggcacaaa gtataaagat ttttattact
     1021 atggttgtaa acataggcag atgataagag gtcataagtg tacattcagt aagcaaatta
     1081 gagaagaatt gttagatgat gctgttgcag aggtgattgt caagatagta agtaatccga
     1141 aatttgcttc tatgatgcaa gaaaaaatca acatgaaggt ggatacctct gaaatagaaa
     1201 aagaaataga taattaccaa aaggaattga gaaagagtca ttctacaaag tttaagctaa
     1261 ttgaggaaat agataattta gatgttgaag ataagcacta taagagaagg aaacaggatt
     1321 tagatgatag actatatcgt atgtatgaca aaatagatga attagaatca tcactaattg
     1381 atgcgaaagc aaagaaacag actattgaag ctgaaaaact tacaggagat aacatatata
     1441 aggttctgat ctattttgat aagctctata aagtcatgaa tgatgtagag cgtaggcagt
     1501 taatttcagc tttgatttct gaaattcaag tttatgaaga aaaacaatct aacggacaat
     1561 ggctaaaatc cattactttt aagctaccaa ttatagaaga aaatctcaac atagatttgg
     1621 acaatgatga acaagttgaa tgtgtaagtt tattagagaa acgtagttaa tcctcaaaag
     1681 gttccccaaa ctttttgagt cccactgacg catcacgctg aggcggtatc actgcttgta
     1741 cgagttgaag catcagcgaa gtaaaagtag atgttcagcc aatgcgacga ggtgagaccg
     1801 aaggtcgata aggtgtgttc tgggtatagg actcgtaaag taagtaggaa atatgatgag
     1861 atgatacaga gagcctgaat tggtcaggtg acgtgcttat ttacattagc ctgataatga
     1921 aaatggagag attatcgatg gttatattgg aattgtacca aaatgattat tctaaagatt
     1981 tagtagcatt tgattccata gaagatggga aagcctttgt agctcaaatc ccgggataca
     2041 ctctggaaac agaagatggt tttgaagtgg agtattttaa tcctaaaaac ataccggatt
     2101 atatggaaat tatttttaac ggaaatattg ttcctttatc taaatttatg ttcgatcctg
     2161 aggaaaatgt aaacattatt tggaaagaga tttcaaattt atccctcaaa aatgatagag
     2221 tgatcgaagg atattcaaaa attgatgcat atgtcgttaa caaccatgaa gttaaagcct
     2281 atgtcgaaac aagagaaacg aactatcgta aagcaaaaga cttcttagaa agccgcggat
     2341 atgaaatcga cagaagcttt ttcggaagtg aagacggtga agcgattctt tacagaaaaa
     2401 aagggatcga agtttggcat ttcttgtgtc acttagaccc aatgtttgta gagattgaag
     2461 atgtagaagg atatgttaaa gaagaagttg gagagattca atagtcaacc aaagttcctt
     2521 gtgagtatat acctttgcta gtaaaagagt caaaacgtta taacgaaggg gggaagaaaa
     2581 cccttgagtg taagtctttt tcttgtttag agggaaagta ttagagtagc cgctcataat
     2641 ttatactaga tttgataa
//
LOCUS       DAVGJL010000158         2645 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.159, whole genome shotgun sequence.
ACCESSION   DAVGJL010000158 DAVGJL010000000
VERSION     DAVGJL010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.159"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            159..1361
                     /gene="rpsA"
                     /locus_tag="VVE42_001931"
     CDS             159..1361
                     /gene="rpsA"
                     /locus_tag="VVE42_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEU3074071.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFIDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSIKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPAQEEGQKEEKRVARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
     gene            complement(1447..2645)
                     /locus_tag="VVE42_001932"
                     /pseudo
     CDS             complement(1447..2645)
                     /locus_tag="VVE42_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          810 a          431 c          598 g          806 t
ORIGIN      
        1 ttaggtaata atcaagataa aaagcaaaat atcttagggt attttatttt tataattgaa
       61 agacgtgaat gatatgaaca tgtccttgcg ggtgcttagg aaaaaaatta taagtatgtc
      121 aagtttaaga aaaacttgat tgttggagga ttttttagat gaacgaattt gaagatttgc
      181 taaatagcgt tagtcaagtt gagactggtg atgttgttag tgctgaagta ttgacagttg
      241 atgcgactca agctaacgtt gcaatctctg gaactggtgt tgaaggtgtc ttgactcttc
      301 gcgaattgac aaacgatcgt gatgcagata tcaatgactt tgttaaagta ggagaagtat
      361 tggatgttct tgtacttcgt caagtagttg gtaaagatac tgatacagtt acataccttg
      421 tatctaaaaa acgccttgaa gctcgcaaag catgggacaa acttgttggt cgcgaagaag
      481 aagttgttac tgttaaagga acgcgtgccg ttaaaggtgg actttcagta gaatttgaag
      541 gtgttcgtgg atttatccca gcttcgatgt tggatactcg tttcgtacgt aacgctgagc
      601 gttttgtagg tcaagaattt gatactaaaa tcaaagaagt taacgctaaa gaaaaccgct
      661 tcatcctttc acgtcgtgaa gttgttgaag cagctactgc agcagctcgc gctgaagtat
      721 tcggtaaatt ggctgttggt gatgttgtaa ctggtaaagt tgctcgtatc acaagcttcg
      781 gcgctttcat cgaccttggt ggtgttgatg gattggttca cttgactgaa ttgtcacatg
      841 aacgtaacgt atcaccaaaa tcagttgtaa ctgttggtga agaaattgaa gtgaaaatcc
      901 ttgatcttaa cgaagaagaa ggacgtgtat cactttctat taaagcaaca gtaccaggac
      961 catgggatgg cgttgagcaa aaattggcta aaggtgatgt agtagaagga acagttaaac
     1021 gtttgactga cttcggtgca tttgttgaag tattgccagg tatcgatgga cttgttcacg
     1081 tatcacaaat ttcacacaaa cggattgaaa atccaaaaga agctcttaaa gttggtcaag
     1141 aagttcaagt taaagttctt gaagttaacg cagatgcaga acgcgtgtca ctttctatta
     1201 aagctcttga agaacgtcca gcccaagaag aaggacaaaa agaagaaaaa cgtgttgctc
     1261 gtccacgtcg tccaagacgt caagaaaagc gtgatttcga acttccagaa acacaaacag
     1321 gattttcaat ggctgatttg tttggtgata tcgaacttta atcaaattga aaattcacaa
     1381 aatcctttgt ttactaaaca aaggattttt ctggctcttt gtcaactgta gtgggttgaa
     1441 aaaaagctaa gctcgagaaa ggacaaattt cgtcctttct tttttgatat tcagagcgat
     1501 gaaaatccgt tttttgaagt tttcaaagtt ccgaaaacca aaggcattgc gcttgataag
     1561 tttgatgaga ttattggtcg cttccagttt ggcattagaa tagtgtagtt gaagggcgtt
     1621 gataatcttt tctttatctt tgaggaaggt tttaaagaca gtctgaaaaa taggatgaac
     1681 ctgctttaga ttgtcctcaa tgagtccgaa aaatttctcc ggtttcttat tctgaaagtg
     1741 aaacagcaag agttgataga gctgatagtg gtgtttcaag tcttgtgaat agctcaaaag
     1801 cttgtctaaa atctctttat tggttaagtg catacgaaaa gtaggacgat aaaatcgctt
     1861 atcactcagt ttacggctat cctgttgtat gagcttccag tagcgcttga tagccttgta
     1921 ttcatgggat tttcgatgaa actgattcat gatttgaaca cgcacacgac tcatagcacg
     1981 gctaagatgt tgtacaatgt gaaagcgatc aagaacgatt tcagcattcg ggagtgaaac
     2041 agtctgggag actgtttcag cctgagccta gaaatttgaa agcgaagctg tttagccaag
     2101 tcatagtaag ggctaaacat atccatagta atgattttga cgcgacatcg gacggctcta
     2161 tcatatttaa gaaagtgatc tcggatggca gcttgtgttc taccttcaag aacagtgatg
     2221 atatcgagct tttcaaaatc ttgcgcaata aagctcatct ccatctcccg attgaaacag
     2281 tcactccccg gactgtttca acgtcccaag acataatctt aggcagattt ctaaaattac
     2341 actcaaagtg gaagtcattg agctttcgaa tgacagttga agttgagatg gaaagctgat
     2401 gggcaatatc ggtcatagaa atcttttcaa ttaacttttg agcaatcttt tggttgatga
     2461 tacgagggat ttggtgattt ttctttacca ggggagtctc agcaaccatc atttttgaac
     2521 agtgatagca cttgaaacga cgctttctaa ggagaattct agtaggcata ccagtcgttt
     2581 caagataagg aattttagaa ggtttttgaa agtcatattt cttcaattgg tttccgcact
     2641 caggg
//
LOCUS       DAVGJL010000159         2587 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.160, whole genome shotgun sequence.
ACCESSION   DAVGJL010000159 DAVGJL010000000
VERSION     DAVGJL010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.160"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            1..927
                     /locus_tag="VVE42_001933"
                     /pseudo
     CDS             1..927
                     /locus_tag="VVE42_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
     gene            1322..2185
                     /locus_tag="VVE42_001934"
     CDS             1322..2185
                     /locus_tag="VVE42_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEU3074072.1"
                     /translation="MIERMELGEFYKELRLARKLKQSDVACEGLTASQLSKFELGQSM
                     LSADKLILAIQGINMNFDEFGHKLNNYQESPHMRIGRKVVDRFAHQDIAALEQLLEEV
                     DQEQMAQTYRRLNAIVIKNALHSLDKSYPLTEEDSEFLTTYLYAIESWTWFELYLFCN
                     TMPFLSNQDLIFLSTSLLEKSKEFKELVHNRWYMKSGYLNIISELMERKLFSYVPIFE
                     AELERMLRPYDVFEKVLWQFLKKMQIFLQTKGSNQKEIEHFIQSLQVLENPQLTALFE
                     LRLQQYKELID"
BASE COUNT          903 a          392 c          479 g          813 t
ORIGIN      
        1 gacagattat gatgaaaaaa ttggtagttt taatacatat gaaatcgaac aaacggtatc
       61 gaagattctg agggtaaaac ctaatggaaa gattatttga aagtcaacag ttccgatcgg
      121 cttttcaaac aaattaaaaa ggctgtttga tacaaaaaat atcatttttg tccctgaatt
      181 tttgagagaa ggttgttcta tatatgataa tttatatcca agtcgcatag ttgttggaga
      241 tgagacagtt gaaggaagaa aaattgcaga gttgttcctt tcgattagta ctcatagtac
      301 tgccaatatt aaaaatgtta tgttagtttc tcctactgaa gcagaagcaa ttaagctttt
      361 ttctaacaca ttcttagctc tccgtgttgc ttttttaatg aactagattc ttttgctgag
      421 aggagaagtt taaatgctga agttgtaata aaaggtgttt gtttagatcc aagaattgga
      481 aatttttata ataatccttc ttttggattt ggaggatatt gtcttcccaa agatactaaa
      541 caattaaaaa aagaatttat agaaataaat gccccagtga tagaagcgat tgatatttca
      601 aacacaaata gaaaacagtt catagttaaa caaatattgg aacgaaagcc aaagatagta
      661 ggaatatata aattggggat gaaatataat tcagataatt acaaagagtc agctatttta
      721 agtataatta atgaactatt gattgtcggt ataaaaattt tagtatatga accaaattta
      781 aacgttagta tagataacgt aatttttgaa aagaattttg aactatttac aaaacaaagt
      841 gatctaattg ttgcaaatag atgggatagg ggccttgaag cttataaaga taaagtttac
      901 acaaggggga tctggataag agattaacta ttgatattaa aaaggcaata atttataaaa
      961 tttgtacaaa aaaatgttaa attttcacat tttcatagta tactgtttaa tagaaagtag
     1021 gttctttgta accgctccat tataaaatcc tttcactctt ccggtatact attaggaaaa
     1081 acataactgg aggatatttt gagctcacac ctcatcgttc cagtggctgt tccccaagct
     1141 cgtgtactgc ccaaaaaagt tagacattct atttagaacg aggattgagt tctgtattgt
     1201 acaaggctcg gtccttttag agtcagctta aggctggctt ttttgccctc ttaaatatca
     1261 gaatattttg acaaatgatt gtaaataatg atataatatg caaaagctag gaggtggtca
     1321 gatgattgag agaatggaat tgggagaatt ttacaaggaa ttgcgcttgg cgagaaaact
     1381 taagcagtca gatgtggcct gtgagggtct aacagcttct cagttgtcca agtttgaact
     1441 aggacagtct atgttatctg cagataagct catattagcc atccaaggga tcaatatgaa
     1501 ttttgatgag tttgggcaca agctcaacaa ctatcaagaa tctccacata tgcgaatcgg
     1561 tcgaaaggtt gtggatcgct ttgcccatca ggatatagct gctttagagc agctattgga
     1621 ggaagtcgat caagaacaga tggcgcagac ctatcgtcgt ttgaatgcca ttgtgattaa
     1681 aaatgcactc cattccttag ataaaagcta cccgctaaca gaggaggata gcgagttttt
     1741 gactacctac ctctacgcta ttgagtcttg gacctggttt gaactctatc ttttttgcaa
     1801 tactatgccc ttcttgagca atcaagatct gatcttttta tcaacctcct tactcgaaaa
     1861 atccaaggaa tttaaagagt tagtacacaa tcgatggtat atgaaaagtg gttatcttaa
     1921 tatcatctca gaacttatgg agcgcaagct attttcttat gttccaatct ttgaagctga
     1981 gctggagcgc atgctccgtc catacgatgt ttttgaaaaa gtattgtggc agtttttaaa
     2041 gaaaatgcag atttttcttc aaaccaaagg aagcaatcaa aaagagattg aacactttat
     2101 ccaatctctg caagtattag aaaatccaca attgacagcc ctttttgaat tgcgtttgca
     2161 gcaatacaaa gagcttatcg attagttaga aatgggaaaa agataataat atcagcaacg
     2221 atcgtcaata atgttaaaac ttgtttcttc atattacttc cctcctcatt agtaataact
     2281 ttattatacc agggaaataa tcaaatctac caaaatcgca aatctgaaat ttctataaga
     2341 aaaaatatca aatatgcgat tttctaaaat aagccaattt ccgtgttata ctgtccttgt
     2401 aaaacatttg aagcaaatcc taggtcgcag aagtggttta caaaaaaata atcaaaggag
     2461 aaaagaaatg cataatctaa tggaacttga attgttacct gaagagccag taattactgt
     2521 atcacgggaa gtttgtaagt ggacttgctc atttactgct ctttaagaat aactggttaa
     2581 aattaag
//
LOCUS       DAVGJL010000160         2569 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.161, whole genome shotgun sequence.
ACCESSION   DAVGJL010000160 DAVGJL010000000
VERSION     DAVGJL010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.161"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            9..698
                     /locus_tag="VVE42_001935"
     CDS             9..698
                     /locus_tag="VVE42_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEU3074073.1"
                     /translation="MEWIRLIGIAIIVVGFILKFDTIATVVLAGLVTALVSGVSLVEF
                     LEILGKEFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFI
                     RELAGFFSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGN
                     FFAQNTFVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKL
                     ISKKTRGGEQK"
     gene            695..1534
                     /locus_tag="VVE42_001936"
     CDS             695..1534
                     /locus_tag="VVE42_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 family protein"
                     /protein_id="HEU3074074.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMPNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPSDSRFIGVLAYVLGMVLFTMIMGNAFAAFTVITAGVGVPFVFALGANPIVA
                     GALAMTAGYCGTLLTPMAANFNALPAALMDMKDQNGVIKAQAGVALVMIVIHIFLMYF
                     LAF"
     gene            1549..2193
                     /gene="pcp"
                     /locus_tag="VVE42_001937"
     CDS             1549..2193
                     /gene="pcp"
                     /locus_tag="VVE42_001937"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEU3074075.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            2361..>2569
                     /locus_tag="VVE42_001938"
     CDS             2361..>2569
                     /locus_tag="VVE42_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEU3074076.1"
                     /translation="MSKKDKKIEIQVADAKVNVGKDSFEGYTLTIGKKVIGEIAELDG
                     QFAIIKNGNVDSFYKKLEKAVEILI"
BASE COUNT          792 a          383 c          563 g          831 t
ORIGIN      
        1 agtttgatat ggagtggatt agattaatag gaatagcaat cattgttgtg ggttttattt
       61 taaaatttga tacaattgca acagtagtct tagctggttt ggttacagct ttagtttcag
      121 gtgtttctct cgttgaattt ttggagattt tgggaaaaga atttagcaat cagcgagtgc
      181 tcacgatttt tatggttacc ttgcctcttg tggggctgtc agaaaccttt ggactcaagc
      241 aacgatcaat cgatttgatt cgaaagatta aaggtctgac agttggaaac ttctatacag
      301 tttatttctt tattcgagag ttagctggtt tcttttcaat tcgtctagga ggacaccctc
      361 agtttgtcag acctttggtt cagcctatgg gagaagcagc tgcagagtct caattaggta
      421 gaaagttaac agaggttgaa gatgagacaa taaaagcgcg tgcggctgcg aatgaaaatt
      481 ttggaaattt ctttgctcaa aatacgtttg taggtgctgg gggagtcctc ttgatagggg
      541 gaacattaga tcagttaggc tatgaaagca attatgcagg gattgcttct acatctatta
      601 ttgttgctgt tataacactt attgtagtgg ggatttacaa ttatttattt gataaaaaat
      661 tgatatcaaa aaagactagg ggaggagaac aaaaatgaca gagttggcaa agcaactatt
      721 agagttgacc tatattgtga ttggttgtca atttctccat acagcctatt gtagttataa
      781 agataaaaca aacccagttc gacttgggac atctgcattt tggactctat tgtctattac
      841 gtttataggt ggttcctata tgccaaatat gagtattggt attattgtaa tcctattatc
      901 gctgttaaca ttgtttaagc aagtccgtat cggaaccttg ccatccttag atgaaatgaa
      961 agccaatatt gaatctaaca ggttgaaaaa taaaattttt attccagtta tgctgatggc
     1021 aatacttgcg ttggtcttag cgcaaatgat tccagaattt agcaagattt cgattagcct
     1081 tgccgccttg tttgctacaa tttctgttct tgtgattacc aatagtcacc ctaagagtct
     1141 gttatcagaa aataatcgaa tgactcagca agtttcaaca agtggtattg ttccttcaga
     1201 tagtcgcttt ataggagttt tggcctatgt tcttggaatg gttctattca caatgattat
     1261 gggaaatgct tttgcagcat tcaccgttat tacagcaggt gttggagttc cctttgtatt
     1321 tgctctggga gctaatccaa ttgtggctgg tgctcttgcc atgacagcag gttattgtgg
     1381 gaccttattg accccaatgg ctgctaattt taacgctcta ccagcagcat tgatggatat
     1441 gaaagatcag aatggcgtta taaaggctca agcaggtgtt gctctagtaa tgattgttat
     1501 tcacatattc ttaatgtact ttctcgcatt ttagtaaagg aaaataaaat gaaaatattg
     1561 gttacaggtt ttaatccttt tggaggtgaa aagattaatc cagctttgga ggctgtaaaa
     1621 ttattaccat ctgagattaa tggggctgaa gttcgctggg tagaaattcc aacggttttt
     1681 tataagtcgt cagaagtttt agaggcagaa atattacgat atcaaccaga tgctgtactt
     1741 tgtattggac aagcaggcgg caggaccggc ttaacacctg aacgagtagc tattaatcaa
     1801 gatgatgctc gcatacctga taacgaaggc aatcaaccaa ttgatacacc gattcgtatt
     1861 gatggagcat cggcctattt tagtagttta cctatcaaag cgatggtaca agctatcaaa
     1921 aaacagggac ttccggcagt tgtatccaat agtgcaggaa cctttgtttg caatcatttg
     1981 atgtaccaag ctctttattt agtggataaa aaattcccta atatgagagc aggtttcatg
     2041 catattccat atatgatgga acaagtagta aataagccga atactgcagg aatgagtcta
     2101 tgtgatattg taagaggcat agaggtagct attgaagcta ttgtagacta caaagataag
     2161 gatttgcagt tagtaggcgg tgagactcac tgataaccta aaaaggatag tcaattatgc
     2221 ttgtttacta actattagct atgctaaatc aattgaggtt gtttacataa aactctatat
     2281 cagagaagcc tgatatagag ttttttcttg ctagttttag gattttcttg taaaatagaa
     2341 aaagtgaaga gaggtatgaa atgagcaaga aagataaaaa aatcgaaatt caagtagcgg
     2401 atgccaaagt taatgttggt aaagacagtt ttgaaggtta tacattgact atcggtaaaa
     2461 aagttatcgg agaaattgcc gaattagacg gacaatttgc cattataaag aatgggaatg
     2521 tcgatagttt ttataaaaaa ttggaaaaag ctgtggaaat tttgattga
//
LOCUS       DAVGJL010000161         2535 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.162, whole genome shotgun sequence.
ACCESSION   DAVGJL010000161 DAVGJL010000000
VERSION     DAVGJL010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.162"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..559
                     /locus_tag="VVE42_001939"
     CDS             <1..559
                     /locus_tag="VVE42_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU3074077.1"
                     /translation="EVPCEIPESWEWVRLNDITSYIQRGKSPKYSNIPIYPVIAQKCN
                     QWSGFSIDLARFIDPETVHSYQKERLLRDGDLMWNSTGLGTLGRLAIYHENKNPYAWA
                     VADSHVTVIRVLSGVINCHFIYNFLSSPIVQSVIEEKASGSTKQKELLTKTIKEYLIP
                     LPPLPEQSRIVDKIEQFFAHIDALI"
     gene            616..1413
                     /gene="psrA"
                     /locus_tag="VVE42_001940"
     CDS             616..1413
                     /gene="psrA"
                     /locus_tag="VVE42_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase PsrA"
                     /protein_id="HEU3074078.1"
                     /translation="MKDFELHLKKAGLAENTVRSYLYGVRFFLENYELKMEDLFEYKG
                     YLLDNFKPKTVNLRLQGVNKYLAFIGHDDLKLKFVKVQQKPFLEDVISHADYLFLKRS
                     LKKDGILKWHFVVWFLGATGARVSELIKLKVEHVEIGYFDIYSKGGKIRRLYIPKKLR
                     NSCLSWLESENRRSGYLFLNKFNEPITARGVAQQLKNYADKYKMNPKVIYPHSFRHLF
                     AKNFLAKYNDIALLADLMGHESIETTRIYLRKTATEQQNIVDKIVNW"
     gene            1424..2029
                     /locus_tag="VVE42_001941"
     CDS             1424..2029
                     /locus_tag="VVE42_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU3074079.1"
                     /translation="MVKLTTCYFCDYGSYYGNIPMNWVVIKIKDIFSMNTGLSYKKGD
                     LSINNKGVRIIRGGNIKPLEFSLLDNDYYIDTQFISSEQVYLKHNQLITPVATSLEHI
                     GKFARIDKDYDGVVAGGFIFQLTPFESSEIISKFLLFNLSSPLFYKQLKAITKLSGQA
                     LYNIPKTTLSELLIPLAPFEEQELITQKVEKLFEKVNQLWK"
     gene            complement(1977..>2535)
                     /locus_tag="VVE42_001942"
     CDS             complement(1977..>2535)
                     /locus_tag="VVE42_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU3074080.1"
                     /translation="NKDETTSYPIYEIPEAWRYIKFASLVNFRIGKTPPRSEATFWGT
                     EIPWVSISDMPISGYVTNTRESISKLALKSKKIDISPKGTLLMSFKLSIGKVAILDIP
                     ATHNEAIISIFPYANKENIIRDYLMIFLPLISTLGDSKDAIKGKTLNSTSISELLIPI
                     SNHEEMKRIISKVDLLFQKVSQLFE"
BASE COUNT          862 a          365 c          442 g          866 t
ORIGIN      
        1 ggaagtacct tgtgaaatac cagaaagttg ggaatgggtg aggttaaacg atataacctc
       61 ctacattcaa agagggaagt ctccaaaata ttcaaatatt cctatatacc ctgtgattgc
      121 tcagaaatgt aatcaatggt ctggcttttc tattgactta gcaagattta ttgatcccga
      181 aacagttcat agttaccaga aagaaagatt actaagggat ggtgatttaa tgtggaattc
      241 aacaggtcta ggtacgttag gacgattagc tatttaccat gaaaataaaa atccttatgc
      301 ctgggctgtt gcagatagcc atgttacagt tataagagtc ttatcggggg ttattaattg
      361 ccatttcatc tataattttc tttcctctcc tattgttcag tcagttattg aagaaaaagc
      421 gtcgggtagt acaaaacaaa aagaattatt aacgaaaact ataaaagaat atttaatccc
      481 cctcccaccc cttcctgaac aatccagaat cgttgataaa atcgaacaat ttttcgccca
      541 tattgacgca ctaatttagt ataatagttt tacgtgcggt ggaatttcta tcaaacataa
      601 gataggagtt ttacgttgaa agattttgaa ttacatttga agaaagctgg tttagctgaa
      661 aatactgtgc gttcgtacct ttatggtgtt cgatttttct tggagaatta cgaactcaaa
      721 atggaagatt tatttgaata caaaggatat ttattagaca attttaaacc taaaactgtc
      781 aatttgcgac tacaaggagt aaataaatat ctggctttta ttggtcatga tgatttgaaa
      841 ttaaaatttg tcaaagtaca gcaaaaaccg tttttagaag atgtcattag ccatgctgat
      901 tacctttttc ttaaacgtag tttgaaaaaa gatggtattt taaaatggca ttttgtcgtt
      961 tggtttttag gtgcaactgg ggctcgtgta agtgaactta ttaaactaaa agtagagcat
     1021 gtagaaatcg gttattttga catttactca aaaggtggta aaattcgtag actgtacatt
     1081 cctaaaaaat taagaaatag ttgtcttagt tggttagaat cagaaaatcg ccgaagtggt
     1141 tatttatttt tgaataaatt taacgaacca attacagcaa gaggagttgc tcaacagtta
     1201 aaaaattatg ctgataaata caaaatgaat cctaaagtaa tttaccctca ttcttttagg
     1261 catttatttg ctaagaattt tttagcgaag tataatgata ttgccttgct tgcagatttg
     1321 atgggacacg aaagtataga aactactcga atttatctaa ggaaaacagc tactgaacaa
     1381 caaaatattg tagataaaat tgttaattgg taaaaaataa caggtggtca aactgactac
     1441 ctgctatttt tgtgattatg gctcttatta tgggaatata cctatgaatt gggttgttat
     1501 aaaaataaaa gatatttttt caatgaatac aggtctttct tacaagaagg gcgatttaag
     1561 cattaataat aaaggtgtta gaattatacg tggtggtaat attaagcctt tagaattttc
     1621 tctgttggat aatgattact acattgatac acaattcatc tcctctgagc aagtttattt
     1681 aaaacataat cagctaataa cacctgtagc aacctcttta gaacatattg gaaagtttgc
     1741 aagaatcgat aaagactatg atggtgttgt ggctggtgga tttattttcc aattaacacc
     1801 attcgaaagt tcagagatta tttcaaaatt tctattattt aacttgtcct ctccgttatt
     1861 ttataaacaa ttgaaagcaa taactaaact atcaggtcaa gctttatata atattcctaa
     1921 aactacacta agcgagctat taattccgtt agctcctttt gaggaacagg aacttattac
     1981 tcaaaaagtt gagaaacttt ttgaaaaagt aaatcaactt tggaaatgat tcttttcatc
     2041 tcttcatgat tagaaatagg gattaataat tcggagatac tggtactatt taatgttttc
     2101 cctttgatag catcttttga atcacctaaa gtagagataa gtggcaaaaa tatcattaag
     2161 taatctctga taatattttc tttgttagca taggggaata tcgatataat ggcttcatta
     2221 tgagtggcag gaatatccaa tatggcaact tttccaatag ataatttaaa actcattaat
     2281 aaagttcctt taggtgaaat gtctattttc tttgatttta atgctaattt agaaatagat
     2341 tctctcgtat tagttacata accagatata ggcatatctg atatagatac ccagggtatt
     2401 tcagttcccc aaaaagtagc ttcactgcgt ggaggagttt ttcctattct gaagttaact
     2461 aggctagcaa atttaatata tctccatgct tctgggattt catatatagg ataagaggtt
     2521 gtttcgtctt tgttc
//
LOCUS       DAVGJL010000162         2482 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.163, whole genome shotgun sequence.
ACCESSION   DAVGJL010000162 DAVGJL010000000
VERSION     DAVGJL010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.163"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(<1..680)
                     /locus_tag="VVE42_001943"
     CDS             complement(<1..680)
                     /locus_tag="VVE42_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3074081.1"
                     /translation="MGKLSSHMWRLNQIIYTKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVFSIWLLHWFIIHTFFLAVVYRRRASDFFLEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLPNWDIGVLFIVDSLNACVLSLLCFMLYALG
                     ANVQMNFACVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDIGRDLFLQLLFLVFLFK
                     SIFYFTRQ"
     gene            complement(690..1442)
                     /locus_tag="VVE42_001944"
     CDS             complement(690..1442)
                     /locus_tag="VVE42_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3074082.1"
                     /translation="MKQFVQFYKKDFLAVLVYFILLLSCVLSSTVYLLRCRQYSIHPN
                     VLEWILVLLQDMTTGVYCFPFTYILFFFYLMNNYFNRLECRIRLKSIKHFTSFSFKLA
                     ALSTGIWTAILFLLIFLIAFSNGFSFSLEIKEVDFLREFYGISIANNASFFIGFFFSY
                     IAYYFFLSLLTISSFSWFKKSNMSLVFLFTFLFVESLFWIYQLNNGIIGLLPIFQYMV
                     NSNPYALIYWLALLSIIIPLTVFSVHRNWRRV"
     gene            complement(1442..2206)
                     /locus_tag="VVE42_001945"
     CDS             complement(1442..2206)
                     /locus_tag="VVE42_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3074083.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKRYLAIVG
                     STAIISSFLVFFLPYLLLLGINLLVTPYQEIYIGDYSGALKELFDSNQLLYSLVTTLW
                     YGVWGAVFSIFGLASALLVKKKIGAIFIPVAYMMVGGIFWAILGLSYLEPVTTLALGY
                     QKDISLSLVSGHLAFILFVSCLVVYGTFFLHSEDYV"
     gene            complement(2302..>2482)
                     /locus_tag="VVE42_001946"
     CDS             complement(2302..>2482)
                     /locus_tag="VVE42_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3074084.1"
                     /translation="YSGSDITPVYSLGSGSRIVMYYNGGGDNYIGSGTRLAMAPQFGN
                     HVRIHTSGSWSPDSY"
BASE COUNT         1030 a          470 c          336 g          646 t
ORIGIN      
        1 ttttgacgag tgaaatagaa aatgctttta aaaagaaaga ctaaaaatag caactgaaga
       61 aataaatctc gtcctatatc tccattccct cgattcaaaa tataaaatag ataattagtt
      121 cgatttccta caaatagacc accaaacaca atcatgagta aaaagaaact aacgcaagca
      181 aagttcattt gtacattcgc tcctagtgcg tataacataa agcaaagcaa ggatagcaca
      241 caagcattca aagaatcaac tataaacaaa actcctatat cccagtttgg taactgtaac
      301 ataaattgaa tcactagaac ttttaccatg attaaaataa gtccataaag aaaacaggtc
      361 caaatctgat acctaatcca gagcttaata gaagaaaatc gaatcacttc cagaaagaaa
      421 tcggatgctc ttctacgata tacaactgct agaaaaaatg tatgaataat aaaccaatga
      481 agcaaccata tagagaagac taaaaaagag ttttgactca gattaggccc ttttaaaatt
      541 tctataacaa gtctatcatt tccttctaac caataccata atcctaaaca aatcaatatc
      601 caaaaaagaa cataacccca aaagtacttg gtatagatta tctgattcaa cctccacatg
      661 tgactactta actttcccat ttccaacttt tacactctcc tccagtttct atgaacagaa
      721 aatacagtca atggaattat gatagatagt aatgcaagcc aataaatcaa tgcatacgga
      781 ttggaattta ccatatactg aaaaattggc aataatccaa ttatcccatt gttcaactga
      841 taaatccaga ataaggattc tacaaataaa aaagtaaaca gaaatactaa gctcatgttt
      901 gattttttaa accaagaaaa actgctaata gtaagtaagg ataaaaagaa atagtatgct
      961 atataagaga aaaaaaatcc tataaagaaa ctagcattgt ttgcaatact tatgccataa
     1021 aattctctta aaaaatcaac ctcctttatc tccaaagaga agctaaaacc attactaaat
     1081 gcaattagaa aaatcaataa aaataaaatc gccgtccaaa tccccgtact aagagctgct
     1141 aatttgaaac taaaactggt aaagtgctta attgatttca gacgaatgcg acactccaac
     1201 ctattaaaat agttattcat caaataaaaa aagaacaata tatatgtgaa cggaaagcaa
     1261 tatactccag tcgtcatatc ttgaagtaaa actaagatcc attctaatac atttggatgg
     1321 attgaatatt ggcgacagcg caataaatat actgtactag ataaaacaca ggatagcagt
     1381 aatataaaat aaaccaatac tgctaagaaa tcttttttat aaaattgaac aaattgtttc
     1441 attatacata gtcctctgaa tgtagaaaaa atgtaccata aacaaccaaa caactaacaa
     1501 ataaaataaa agcaagatgc ccactaacta aggaaagact gatatctttc tgatatccca
     1561 aagctaatgt tgtcacaggt tctaagtaag atagccctaa aatagcccaa aaaataccac
     1621 caaccatcat ataggcaact gggatgaaaa tagctcctat ttttttcttc actagcaaag
     1681 cactagctag tccaaaaata gagaacacag cgccccaaac tccataccag agagtcgtca
     1741 caagactata gagcaactga ttagaatcaa ataattcttt taaggcacca ctataatctc
     1801 caatataaat ttcctgataa ggagtcacta aaagattaat tcctaataat aataaatagg
     1861 ggagaaaaaa gactagaaaa gaagaaataa ttgcagtact acctacaata gctagatacc
     1921 tctttttaga aattcggcac aattgtgctg ttagaaaatg actctcagca tcctctatta
     1981 tctgactaga atagggcagt gtacagataa gtgcagctac taggctaatc ggtgaaaatc
     2041 ctcgaataga agaagcggca aaaaaaatcg ataaaccttc aattttataa ataccattga
     2101 aagcaaggaa atttcctaaa ctcatgcaaa gaagggtcaa aaataaagac atataaaatc
     2161 gaggtgattg aacgactcgt acaagattac aaatgaaaaa ttccatcctt actcctcctt
     2221 ataataaaaa tagggtgtag cattcttttt tcatgctaca cccacaatca accatcttta
     2281 aggcttactc tgacaagtaa gttaataaga atctggactc caagaacctg aagtatgaat
     2341 tcttacatga tttccaaatt gtggcgccat agctaatcta gtaccagaac caatataatt
     2401 gtcaccacct ccattatagt acatgacaat cctagagcca gaccccaatg aatataccgg
     2461 ggtaatatct gacccactat ag
//
LOCUS       DAVGJL010000163         2449 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.164, whole genome shotgun sequence.
ACCESSION   DAVGJL010000163 DAVGJL010000000
VERSION     DAVGJL010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.164"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            89..628
                     /locus_tag="VVE42_001947"
     CDS             89..628
                     /locus_tag="VVE42_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU3074085.1"
                     /translation="MKLLSIAISSYNAAAYLHYCVESLVIGGEQVGILIINDGSQDQT
                     QEIAECLASKYPNIVRAIYQKNKCHGGAVNRGLAEASGRYFKVVDSDDWVDPRAYLKI
                     LETLQELESKGQEVDVFVTNFVYEKEGQSRKKSMSYDSVLPVRQIFGWDQVGNFSKGQ
                     YTMMHSLIYRTALLRASQF"
     gene            695..1105
                     /locus_tag="VVE42_001948"
     CDS             695..1105
                     /locus_tag="VVE42_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEU3074086.1"
                     /translation="MYYLPVDFYRYLIGREDQSVNEQVMIKCIDQQLKVNRLLVDQLD
                     LSQVSHPKMREYLLNHIEITTVISSTLLNRSETAEHLAKKRQLWTYIQQENPKVFQAI
                     RKTMLSRLTKHSVLPDRKLSNVVYQITKSVYGFN"
     gene            1221..>2449
                     /gene="pavB"
                     /locus_tag="VVE42_001949"
     CDS             1221..>2449
                     /gene="pavB"
                     /locus_tag="VVE42_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HEU3074087.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADV
                     VNPTPGQVLPEETSGTKEGDLSEKPGDTVLTQAKPEGVTGNTNSLPTPTERTEVSEET
                     NSSSLDTLFEKDEEAQENPELTDALKETVDTADVDGTQASPAETTPEQVKGGVKENTK
                     DSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRANGTQTYKATVKVYGNKDGK
                     ADLTNLVATKNVDININGLVAKETVEKAVKDNVKDSIDVPAAYLEKAKGEGPFTAGVN
                     HVIPYELFAGDGMLTRLLLKASDKAPWSDNGEAKNPALSPLGENVKTKGQYFYQVALD
                     GNVAGKEKQALIDQFRA"
BASE COUNT          752 a          485 c          548 g          664 t
ORIGIN      
        1 aacatctata cggcaaggcg acgttgacgc ggtttgaaga gattttcgaa gagtataaga
       61 aaaaatcagt cccctaaagg agcagattat gaagttattg tctatcgcca tttctagcta
      121 taatgcagca gcctatcttc attactgtgt ggagtcgcta gtgattggtg gtgagcaagt
      181 tgggattttg attatcaatg acgggtctca ggatcagact caggaaatcg ctgagtgttt
      241 agctagcaag tatcctaata tcgttagagc catctatcag aaaaataaat gccatggcgg
      301 tgcggtcaat cgtggcttgg cagaggcttc tgggcgctat tttaaagtag ttgacagtga
      361 tgactgggtg gatcctcgtg cctacttgaa aattcttgaa accttgcagg aacttgagag
      421 caagggtcaa gaggtggatg tctttgtgac caattttgtc tatgaaaagg aagggcagtc
      481 tcgtaagaag agtatgagtt acgattcagt cttgcctgtt cggcagattt ttggctggga
      541 ccaggtcgga aatttctcca aaggccagta taccatgatg cactcgctga tttatcggac
      601 agctttgttg cgtgctagcc agttctaatt gcctgaacat actttttatg tcgataatct
      661 ctttgtcttt acgccccttc agcaggtcaa gaccatgtac tatctgcctg tcgatttcta
      721 tcgttatttg attgggcgtg aggaccagtc tgtcaatgag caagtgatga ttaagtgcat
      781 tgaccagcaa ctcaaggtca atcgactctt ggtagaccag cttgatttgt cccaagtgag
      841 tcatcctaaa atgcgagaat atctgctgaa tcatattgaa atcacgacgg tgatttccag
      901 taccctgctc aaccgatctg aaacagcgga gcatctggca aaaaaacgcc aattgtggac
      961 ctatattcag caggaaaatc caaaagtctt tcaggctatt cgtaagacca tgttgagccg
     1021 tttgaccaaa cattctgtct tgccagatcg caaactgtcc aatgtcgtct atcaaatcac
     1081 caaatctgtt tatggattta attaatataa gtgttttata agagggattt aagaaaaatt
     1141 ttaacttttt cttagtcctt tttaatttca ggagattata ctagagtcat caaataaaga
     1201 aagactctaa ggagaatcct atgaaattca atccaaatca aagatatact cgttggtcta
     1261 ttcgccgtct cagtgtcggt gttgcctcag ttgttgtggc tagtggcttc tttgtcctag
     1321 ttggtcagcc aagttctgta cgtgccgatg tggtcaatcc gaccccaggt caagtcttac
     1381 ctgaagagac atcgggaacg aaagagggtg acttatcaga aaaaccagga gacaccgttc
     1441 tcactcaagc gaaacctgag ggcgttactg gaaatacgaa ttcacttccg acacctacag
     1501 aaagaactga agtgagcgag gaaacaaact cttctagtct ggatacactt tttgaaaaag
     1561 atgaagaagc tcaagaaaat ccagagctaa cagatgcctt aaaagaaact gtagatacag
     1621 ctgatgtgga tgggacacaa gcaagtccag cagaaactac tcctgaacaa gtaaaaggtg
     1681 gagtgaaaga aaatacaaaa gacagcatcg atgttcctgc tgcttatctt gaaaaagctg
     1741 aagggaaagg tcctttcact gccggtgtaa accaagtaat tccttatgaa ctattcgctg
     1801 gtgatggtat gttaactcgt ctattactaa aagcttcgga taatgctcct tggtctgaca
     1861 atggtactgc taaaaatcct gctttacctc ctcttgaagg attaacaaaa gggaaatact
     1921 tctatgaagt agacttaaat ggcaatactg ttggtaaaca aggtcaagct ttaattgatc
     1981 aacttcgcgc taatggtact caaacttata aagctactgt taaagtttac ggaaataaag
     2041 acggtaaagc tgacttgact aatctagttg ctactaaaaa tgtagacatc aacatcaatg
     2101 gattagttgc taaagaaact gtcgaaaaag ctgttaaaga taacgttaaa gacagtatcg
     2161 atgttccagc agcctaccta gaaaaggcca agggtgaagg tccattcaca gcaggtgtca
     2221 accatgtgat tccatacgaa ctcttcgcag gtgatggcat gttgactcgt ctcttgctca
     2281 aggcatctga caaggcacca tggtcagata acggtgaggc taaaaaccca gccctatctc
     2341 cactaggcga aaacgtgaag accaaaggtc aatacttcta tcaagtagcc ttggacggaa
     2401 atgtagctgg caaagaaaaa caagcgctca ttgaccagtt ccgagcaaa
//
LOCUS       DAVGJL010000164         2359 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.165, whole genome shotgun sequence.
ACCESSION   DAVGJL010000164 DAVGJL010000000
VERSION     DAVGJL010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.165"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            84..>2359
                     /locus_tag="VVE42_001950"
     CDS             84..>2359
                     /locus_tag="VVE42_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase/lanthionine synthetase C family
                     protein"
                     /protein_id="HEU3074088.1"
                     /translation="MNNDEWVYQYPIGKFVERQGWKIHISSEYNSSHELLQDVAKICH
                     EMRIPFKHLSTEDKFIMRNGKLVSRGFSGKFITCYPNQNELESVLQRLESALKQYNGP
                     YILSDKRWDEAPIYLRYGVFRPSRDDEKKVAIDELIVGDEVVKDERLPVFKIPKGIVP
                     PDFLNKWLDKKDKKQGDFPFIIDNAIRFSNSGGIYNARLKEDGKKIILKEARPYTGLG
                     FDGTYSSERLASECKALKILNEWSETPKIYWHGKIWEHTFLGIEHMKGVPLNRWVTNN
                     FPLYEVADKTKDYLLRVSKIVEKLIDLTNKFHSENVYHQDLHLGNILVKDEDEISIID
                     WEQAVFSNDEKVVHKVAAPGFRAWRETLPSEIDWYGIRQIAHYLYMPLVTTSDLTYNY
                     VSQTRIEGKKLFESLGYTREHIDYVESLLSYLDSKCPQIENISRKKVLKPMHEIRTIE
                     SEQDIQDFIIKLLRGFTLTYGQWRKEFQSRFFPVHYYGLNFNQGIAFSDLAILWSYQQ
                     LAKKVKNFKFDDYYEIRTQVINEAVNNFKKSSLSGLFDGKIGTIWLIYEFGEIDRAVE
                     LFTTHFIEIFENSQNKNLYSGQAGILLVGLYFLSKGGIDNKLGEEILIRLREYTLNYI
                     ENPETFCKVGASDVQSNDPYENFGGLLYGHAGVAWLFGEAYKLTGESIYKNGLELAVD
                     KELVAYKVDSNNSLQYSQGHRLLPYLATGSAGLLLLINRNKEILSSKYLKYLTSLERA
                     TDVVFCVLPGLFNGFCGL"
BASE COUNT          833 a          298 c          482 g          746 t
ORIGIN      
        1 ctttcttttt cactaataat gattatagta cagatactag cataaagtac caagtaagtc
       61 tgccttttaa ttggcacgaa gtaatgaata atgatgagtg ggtttatcaa taccctattg
      121 gaaaatttgt tgaaagacaa gggtggaaaa tacacattag ttcagagtat aacagttcac
      181 atgaattgtt acaagatgta gcaaaaattt gccatgaaat gcgaattcca tttaaacatc
      241 ttagtacaga agacaaattt atcatgcgaa acgggaaatt ggttagtcga ggattttctg
      301 gtaaatttat tacatgttat ccaaatcaaa atgaattaga gtcggttttg caaaggttag
      361 aaagtgcctt aaagcaatat aatgggccat atattttaag cgataaacgt tgggatgaag
      421 caccaatata tctgaggtat ggtgtgttta ggccgtcaag ggatgatgaa aagaaggttg
      481 ctattgatga attaattgtt ggcgatgaag ttgttaaaga tgaacgttta ccagtattta
      541 aaattccaaa aggtatagtg ccccctgact ttttgaataa atggcttgac aagaaagata
      601 aaaaacaggg agactttccc tttattatag ataatgcaat tagattttca aacagtggtg
      661 ggatatataa tgctaggcta aaagaagatg ggaaaaaaat aattttaaaa gaggctagac
      721 cgtatacagg tctaggattt gatggtactt attcttcaga gagattagct tcggagtgca
      781 aagcactaaa aattctaaat gaatggagtg agacgcctaa aatatactgg cacggtaaga
      841 tctgggagca caccttctta ggaattgaac atatgaaggg cgttccacta aatagatggg
      901 ttaccaataa ttttccttta tatgaagttg ctgataagac caaagattat ctattgagag
      961 tttcaaagat agtagaaaaa ctcatcgatt taacgaataa gtttcattct gaaaatgtat
     1021 atcatcaaga tttacattta ggaaatatct tggttaagga tgaagatgag attagtataa
     1081 tagactggga acaggctgtc ttttcaaatg atgaaaaagt tgtacataaa gttgcagccc
     1141 ctggttttag agcatggcga gaaacacttc ccagtgagat agattggtat ggtattagac
     1201 aaatagctca ttatttatac atgccactag taactacatc ggatttgaca tacaactatg
     1261 taagtcaaac caggatagaa ggaaaaaaac tatttgagtc tttgggttat acaagagaac
     1321 atatagatta tgtagaaagt ttattgtcat atctagattc taaatgtcca cagattgaga
     1381 atatttccag gaaaaaagtt ttaaaaccca tgcatgagat tagaactatc gagagtgagc
     1441 aagatataca agattttatt ataaaattat tgagagggtt tacgctaact tatggacagt
     1501 ggcgtaaaga atttcaatca agattcttcc cagttcatta ttatggtctg aattttaatc
     1561 aaggaattgc gttttcagat ttagctatcc tttggagtta tcaacaacta gctaagaaag
     1621 taaaaaattt caagtttgat gactattatg agattcgaac acaggttatt aatgaggcag
     1681 taaataattt taagaaatcg tccctttctg gtctttttga tggtaaaatt ggtacaatct
     1741 ggctcattta tgaatttggg gagatagatc gggcagttga actttttaca actcatttta
     1801 tagagatatt tgagaattca caaaataaaa atttatatag tgggcaggct ggaattttac
     1861 tggttggact gtattttctt agtaaagggg gaattgataa taaactagga gaagaaatac
     1921 taattaggct tagagaatat actttaaatt atatagaaaa tccagaaact ttttgtaaag
     1981 ttggcgctag tgatgttcag agtaacgatc cttatgagaa ttttggtgga ttactatatg
     2041 gtcacgcagg ggtagcttgg ctcttcggtg aagcttataa attaactggt gaaagtatct
     2101 ataaaaatgg tctagaattg gctgtagata aagaattagt tgcttataaa gtagatagta
     2161 ataattcatt gcagtacagc caaggtcata gattactacc atatctagca actggaagtg
     2221 ctggcctgct tttacttatt aatagaaata aagaaatctt gtcaagcaaa tacttaaaat
     2281 atttaacttc tttagagagg gcaactgatg tagttttttg tgtattacca ggtttattta
     2341 acggattttg tggtcttga
//
LOCUS       DAVGJL010000165         2304 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.166, whole genome shotgun sequence.
ACCESSION   DAVGJL010000165 DAVGJL010000000
VERSION     DAVGJL010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.166"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            55..822
                     /locus_tag="VVE42_001951"
     CDS             55..822
                     /locus_tag="VVE42_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEU3074089.1"
                     /translation="MIGISLIPDLYNIIFLSSMWDPYGQLSDLPVAVVNNDKEASYNG
                     NTMAIGKDMVSNLKENKTLDFHFVDEEEGKKGLEDGDYYMVVTLPSDLSEKTTTLSNI
                     QSTAAYQSLTSEQQTEISDSVSQNLTASIQSAQSIVALVQDLQGSLENLQNQSSNLST
                     LKNQSNQVSPITSTSLIGLSSGLTEIQGDVTSKLVPASQSIASGVNAYTTGVDKVSQD
                     ASQLSEKNATLTGSLNKLVSGSNTLTQKSSRLTAGVG"
     gene            907..1245
                     /locus_tag="VVE42_001952"
     CDS             907..1245
                     /locus_tag="VVE42_001952"
                     /inference="COORDINATES: protein motif:HMM:TIGR03057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3074090.1"
                     /translation="MADGSGKLAEGGTKLTSGLEDLQTGLASLGQGLGNASDQLKSVS
                     TESKNAEILSNPLNLSKTDNDQVPVNGIAIAPYMISVALFFAAISTNMIFAKLPSGRH
                     PESRWAWLKS"
     gene            1273..1587
                     /locus_tag="VVE42_001953"
     CDS             1273..1587
                     /locus_tag="VVE42_001953"
                     /inference="COORDINATES: protein motif:HMM:TIGR03062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HEU3074091.1"
                     /translation="MAGILVYGGVQLIGLTANHEMRIFILIILTSLVFMSMVTTLATW
                     NSRIGAFFSLILLLLQLASSAGTYPLALTNDFFRSINPWLPMSYSVSGLRQTISINKS
                     FS"
     gene            complement(1688..>2304)
                     /locus_tag="VVE42_001954"
     CDS             complement(1688..>2304)
                     /locus_tag="VVE42_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEU3074092.1"
                     /translation="SSSNEDAAIQYQIEVPENSQVYLSFINLHFSNDKQKKVDILVNG
                     EKKTFTTDNVFSFFNLGYTKEKKTFNINVSFPGNSQVSFESPTFYRLDTKTFTEAIQK
                     IKEQPVTVSTSKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQAQTGFMKV
                     DIPKGKGTITLSFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
BASE COUNT          755 a          363 c          439 g          747 t
ORIGIN      
        1 ctaagtttaa agaatggaaa gcaattttaa aaaaaaccaa cctttattat tgtcatgatc
       61 gggatttctc ttattccaga tctgtacaat atcatatttt tgtcatcaat gtgggatcca
      121 tatgggcaat tgtctgactt acctgtggca gttgtaaata atgataaaga ggcttcctat
      181 aatggtaata ctatggcaat aggaaaagac atggtgtcca atttaaaaga aaataaaacc
      241 ttggattttc attttgtaga tgaagaggaa ggaaagaagg gattggaaga tggcgattac
      301 tatatggtag tgactttacc aagtgattta tctgaaaaaa caactacatt atccaatatt
      361 caatcgacag cagcttatca atcattgaca agtgagcaac aaactgagat aagtgattct
      421 gtatctcaaa atttaactgc tagtattcaa tcggctcagt caattgtagc tttagtacaa
      481 gatttacagg gaagtttaga aaacttacaa aatcaatctt ctaatctttc gactttaaaa
      541 aatcaatcta atcaagtatc acctattact tctacttctt tgataggatt gtcaagtgga
      601 ttaacagaga tacaaggaga tgttactagc aaattagttc ctgccagtca gtcgattgca
      661 tcaggtgtaa acgcatatac tacaggtgtt gataaagttt ctcaggacgc aagtcaacta
      721 agtgaaaaaa atgccacctt gacaggtagt ttgaataaac tagtttcagg ctcaaacacc
      781 ttgacacaaa aatcttctag attgacagca ggagttggtt aattacaatc aggatctggg
      841 caattagcag acaaatccag tcagttactt tcaggtgctt ctccattaga gaatagagct
      901 aataaattgg cagatggatc tgggaaacta gcagaaggtg gaacaaagtt aacttctgga
      961 ttggaagatt tacagacagg acttgcttct ttaggacaag gactaggtaa tgctagtgat
     1021 caactcaaat cagtatcaac agaatctaaa aatgcagaga ttttgtcaaa tccactcaat
     1081 ctttcaaaaa cagacaatga tcaagttcct gtaaatggaa tcgcaatagc tccttatatg
     1141 atatcagttg ctcttttttt tgcagcaata tcaacaaata tgatatttgc gaaattgcct
     1201 tcaggacgtc atccagagag ccgttgggct tggttgaaat cttgagctga aataaatggt
     1261 attatagctg ttttggcagg aattttggta tatggaggag ttcagcttat tggtttaact
     1321 gctaatcatg agatgagaat atttattctc atcatcctaa caagtttagt attcatgtct
     1381 atggtgacca ctttagcaac gtggaatagc cgtataggag cttttttctc acttattttg
     1441 cttttactac agttagcatc aagtgcaggt acttatccac ttgctttgac aaatgatttc
     1501 tttagatcta ttaatccctg gttaccaatg agctattcag tttcgggatt acgacaaaca
     1561 atctctatca acaagtcatt ttcctagctg tcatactagc tctatttact ggtttaggta
     1621 tgctagccta tcaacataag aaaatggaag aagattaaaa aaatcgaccg attaactggt
     1681 cgatttttta tgccttagat gactttcgtc tgtgattata gattccaaat agtaagagag
     1741 aagtaaagga acagattgct ccagtaataa aaccattggg aatgaaggaa agtgtaatag
     1801 ttcctttccc cttgggaatg tcaactttca taaatccagt ttgagcttgt ttaatttcta
     1861 ttttcttacc atcttggtag gcagaccaac ctttgtcata aggaatggtg aagaaaatag
     1921 atgtatcttg ttggacatca tatgtagcaa aaaccttgtt tttagaagtt gatactgtga
     1981 caggttgttc tttaattttt tgaattgcct cggtgaaagt tttggtatct aaacgataga
     2041 aggtaggaga ttcaaatgat acttgtgaat ttccagggaa actaacattg atattgaaag
     2101 tttttttctc tttagtatat cctagattaa agaaggagaa gacattatca gttgtaaaag
     2161 tctttttttc accatttaca aggatgtcaa ccttcttttg tttatcgtta gaaaagtgaa
     2221 ggtttatgaa agagagataa acttggctgt tttctggaac ttcaatttga tactggattg
     2281 ctgcatcttc atttgaagaa cttg
//
LOCUS       DAVGJL010000166         2303 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.167, whole genome shotgun sequence.
ACCESSION   DAVGJL010000166 DAVGJL010000000
VERSION     DAVGJL010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.167"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            231..1235
                     /locus_tag="VVE42_001955"
     CDS             231..1235
                     /locus_tag="VVE42_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEU3074093.1"
                     /translation="MNASFRGSASFIRDNVLVTAAHVVYDRDSRTEADSVYVIPAATP
                     NENPVGKIKVKEVRYLKEFQNTASNELTTYDLAVLILEEPIGAQLGTLGLPNNLGNLS
                     GTGATITGYPAVNSAKQMYTDSKDIVKDTGDFLMYQIDTLGGASGAGVYDSSFRLIGV
                     HVSGSSAGQMNSAVKLNEKALSFIYSVIQGHSIDGWKLISGTWYYYKNSTKQTGWQKI
                     NDTWYYLDASGKMRTDWEKVNGKWYYFESSGSMATGWKYVRGNWYYLDNKNGDMKTGW
                     YKDGSTWYYLDPTNGNMKKGWIKVGGNWYYLNSSGAMVTGSQTIDGKVYNFASSGEWI
                     "
     gene            1254..2234
                     /locus_tag="VVE42_001956"
     CDS             1254..2234
                     /locus_tag="VVE42_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU3074094.1"
                     /translation="MKLLKKTMQVGLTAFFFGLLATNTVFADTTGGQFVDKDNRKYYV
                     KDDHKAIYWHKIDGKTYYFGNKGEMVVGWQYLEIPGTGYRDNLLDNQPVNEIGLQHKW
                     YYFGQDGSLQEYVGWKELDAKDSLNVGKEYGEPFEGPEVLKYANYYFDKDHSLKTGWF
                     YDQSNWYYLAKTGYLGKDYLGGERRAGWIYDGSSWYYLDTTTAAMQTGWQYLGNNWYY
                     LRSSGAMATGWMKDGSTWYYLDGQNGDMKTGWAYVGNSWYYLRSSGAMSTGWMKDGST
                     WYYLNASNGDMKTGWFQVNGKWYYAYGSGALAVNTTVGGYYVNYNGEWVK"
BASE COUNT          769 a          307 c          514 g          713 t
ORIGIN      
        1 ctgctttgac tgttatttct agtacaggtg aagaatatga ggtaagtgaa acattacaag
       61 agagtccagg aactaataat ttttcacttc ccgatatttc accgacttat ggtaaatatt
      121 atacaaatca gtcagaagtc attattggca acaatgattt agttaagatt gggaatactt
      181 tacagtatcc ttattctaca tcagcttacg taaaagcaga atttaatagt atgaatgcta
      241 gttttagagg cagtgctagt ttcataagag ataatgtttt ggttacagca gctcatgttg
      301 tttatgatcg tgattctaga acagaggcgg attctgtata tgttatccct gcggctactc
      361 cgaacgaaaa tcctgtagga aaaataaagg taaaagaagt tcgttatcta aaagaatttc
      421 aaaacacagc ttcaaatgag cttactacgt atgatttagc agttttgatt ttggaagaac
      481 caattggcgc tcaattaggt acactaggtc ttccgaataa tctagggaat ctttcaggta
      541 caggggctac tattactgga tatcctgctg taaatagtgc taagcaaatg tatactgact
      601 ctaaggatat tgtaaaagat actggagatt ttctaatgta tcaaatagat actctaggag
      661 gtgctagtgg agctggcgta tatgacagca gttttagatt gattggtgtt catgtgtctg
      721 gtagttctgc tggacagatg aattctgctg ttaagttaaa tgaaaaggct ttatcattta
      781 tctactctgt tattcaagga cattctattg atggttggaa attgattagt ggtacatggt
      841 actattataa gaacagtact aaacaaacag gttggcagaa aataaatgat acttggtact
      901 atctagatgc ttcaggtaag atgcgtacag attgggaaaa agtaaatgga aaatggtatt
      961 actttgaatc atctggttcg atggcaacag gttggaaata tgtacgaggc aactggtatt
     1021 acttagataa taaaaatggt gatatgaaga ctggttggta taaagatggt tcaacatggt
     1081 actatctaga tcctactaat ggaaacatga aaaaaggttg gataaaagta ggaggaaact
     1141 ggtattatct aaattcaagt ggagcaatgg ttacaggtag ccaaactatc gatggtaaag
     1201 tttataattt cgcttcatct ggtgagtgga tctaatattg gaggggtata taaatgaaac
     1261 ttttgaaaaa aactatgcaa gttggactaa cagcattttt ctttggtttg ttagctacaa
     1321 atacagtatt tgcagatacc acaggtggcc aatttgttga taaggacaat agaaaatatt
     1381 atgtaaaaga tgatcataaa gcaatctatt ggcataaaat agacggaaaa acttactatt
     1441 ttggtaataa aggagaaatg gtagttggat ggcaatactt agaaattcct ggaacaggtt
     1501 atcgtgataa tttattagat aaccaaccag ttaatgaaat aggccttcaa cacaagtggt
     1561 actattttgg acaagatgga agtctccaag aatatgttgg ttggaaggaa ttagatgcta
     1621 aggattcatt aaatgttggc aaagagtatg gtgaaccatt tgaaggtcct gaagttctta
     1681 aatatgcaaa ttattacttt gataaagatc attctttaaa gacaggttgg ttttatgatc
     1741 aatccaactg gtactactta gcaaaaacag gttatttagg aaaagactat cttggtggtg
     1801 aaagacgtgc aggttggata tatgacggtt caagctggta ttatctagat acaacaactg
     1861 ctgcaatgca aacaggatgg caatatctag gtaataactg gtattatctc cgttcatcag
     1921 gtgctatggc aactggttgg atgaaagatg gttcaacttg gtattatcta gatggtcaaa
     1981 atggtgatat gaagacaggt tgggcttatg ttggcaacag ttggtactac cttcgttcat
     2041 caggtgcaat gtctactggt tggatgaaag atggttcaac ttggtactac ctaaatgcaa
     2101 gtaatggaga tatgaagaca ggctggttcc aagtcaatgg taaatggtac tatgcctatg
     2161 gttcaggtgc tttagctgtt aataccacag taggtggtta ttacgtaaac tacaacggtg
     2221 aatgggttaa gtaatgaagg ctaatgttaa actatagttg atacttaaca tagtaaaaga
     2281 gagaatcaga gtgtgattct ctc
//
LOCUS       DAVGJL010000167         2272 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.168, whole genome shotgun sequence.
ACCESSION   DAVGJL010000167 DAVGJL010000000
VERSION     DAVGJL010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.168"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            154..>2272
                     /locus_tag="VVE42_001957"
     CDS             154..>2272
                     /locus_tag="VVE42_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEU3074095.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIFSEE
                     LLMKDPNYKLKDEDIVNEVKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTPRNDGAVALARSQGRYTTDDGYIFNVSDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRGNLSNSRTYRRQNSDNTSRTNWVPSVSNPGTTNTNTSNNSN
                     TNSQASQSNDIDSLLKQLYKLPLSQRHVESDGLIFDPAQITSRTARGVAVPHGNHYHF
                     IPYEQMSELEKRIARIIPLRYRSNHWVPDSRPEQPSPQSTPEPSPSLQPAPNPQPAPS
                     NPIDEKLVKEAVRKVGDGYVFEENGVSRYIPAKDLSAETAAGIDSKLAKQESLSHKLG
                     AKKTDLPSSDREFYNKAYDLLARIHQDLLDNKGRQVDFEALDNLLERLKDVSSDKVKL
                     VDDILAFLAPIRHPERLGKPNAQITYTDDEIQVAKLAGKYTTEDGYIFDPRDITSDEG
                     DAYVTPHMTHSHWIKKDSLSEAERAAAQAYAKEKGLTPPSTDHQDSGNTEAKGAEAIY
                     NRVKAAKKVPLDRMPYNLQYTVEVKNGSLIIPHYDHYHNIKFEWFDEGLYEAPKGYTL
                     EDLLATVKYYVEHPNERPHSDNGF"
BASE COUNT          762 a          446 c          480 g          584 t
ORIGIN      
        1 gtggtcagaa caaaaaatac ttaaatggtt cataaaattc ttgacaagtt ggagatttag
       61 gagtaaacta ttaaccagtt aagtaataga gaggagtttc tgcaatttag aaatgaattg
      121 caactagaaa tatcaaatag aaagagagtt tcgatgaaaa ttaataagaa ataccttgtt
      181 ggttctgcgg cagctttgat tttaagtgtt tgttcttacg agttgggact gtatcaagct
      241 agaacggtta aggaaaataa tcgtgtttcc tatatagatg gaaaacaagc gacgcaaaaa
      301 acggagaatc tgacaccaga tgaggttagc aagcgtgaag gaatcaatgc tgagcaaatc
      361 gtcatcaaga taacagacca aggctatgtc acttcacatg gcgaccacta tcattattac
      421 aatggtaagg ttccttatga tgcgattttc agtgaagaat tactcatgaa agatccaaac
      481 tataagctaa aagatgagga tattgttaat gaggtcaagg gtggatatgt tatcaaggta
      541 gatggaaaat actatgttta ccttaaggat gctgcccacg cggataacgt ccgtacaaaa
      601 gaggaaatca atcgacaaaa acaagaacat agtcaacatc gtgaaggtgg gactccaaga
      661 aacgatggag ctgttgcctt ggcacgttcg caaggacgct atactacaga tgatggttat
      721 atctttaatg tttctgatat catagaggat actggtgatg cttatatcgt tcctcatgga
      781 gatcattacc attacattcc taagaatgag ttatcagcta gcgagttggc tgctgcagaa
      841 gccttcctat ctggtcgggg aaatctgtca aattcaagaa cctatcgccg acaaaatagc
      901 gataacactt caagaacaaa ctgggtacct tctgtaagca atccaggaac tacaaatact
      961 aacacaagca acaacagcaa cactaacagt caagcaagtc aaagtaatga cattgatagt
     1021 ctcttgaaac agctctacaa actgcctttg agtcaacgcc atgtagaatc tgatggcctt
     1081 attttcgacc cagcgcaaat cacaagtcga accgccagag gtgtagctgt ccctcatggt
     1141 aaccattacc actttatccc ttatgaacaa atgtctgaat tggaaaaacg aattgctcgt
     1201 attattcccc ttcgttatcg ttcaaaccat tgggtaccag attcaagacc agaacaacca
     1261 agtccacaat cgactccgga acctagtcca agtctgcaac ctgcaccaaa tcctcaacca
     1321 gctccaagca atccaattga tgagaaattg gtcaaagaag ctgttcgaaa agtaggcgat
     1381 ggttatgtct ttgaggagaa tggagtttct cgttatatcc cagccaagga tctttcagca
     1441 gaaacagcag caggcattga tagcaaactg gccaagcagg aaagtttatc tcataagcta
     1501 ggagctaaga aaactgacct cccatctagt gatcgagaat tttacaataa ggcttatgac
     1561 ttactagcaa gaattcacca agatttactt gataataaag gtcgacaagt tgattttgag
     1621 gctttggata acctgttgga acgactcaag gatgtctcaa gtgataaagt caagttagtg
     1681 gatgatattc ttgccttctt agctccgatt cgtcatccag aacgtttagg aaaaccaaat
     1741 gcgcaaatta cctacactga tgatgagatt caagtagcca agttggcagg caagtacaca
     1801 acagaagacg gttatatctt tgatcctcgt gatataacca gtgatgaggg ggatgcttat
     1861 gtaactccac atatgaccca tagccactgg attaaaaaag atagtttgtc tgaagctgag
     1921 agagcggcag cccaggctta tgctaaagag aaaggtttga cccctccttc gacagaccat
     1981 caggattcag gaaatactga ggcaaaagga gcagaagcta tctacaaccg cgtgaaagca
     2041 gctaagaagg tgccacttga tcgcatgcct tacaatcttc aatatactgt agaagtcaaa
     2101 aacggtagtt taatcatacc tcattatgac cattaccata acatcaaatt tgagtggttt
     2161 gacgaaggcc tttatgaggc acctaagggg tatactcttg aggatctttt ggcgactgtc
     2221 aagtactatg tcgaacatcc aaacgaacgt ccgcattcag ataatggttt tg
//
LOCUS       DAVGJL010000168         2263 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.169, whole genome shotgun sequence.
ACCESSION   DAVGJL010000168 DAVGJL010000000
VERSION     DAVGJL010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.169"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..316
                     /locus_tag="VVE42_001958"
     CDS             <1..316
                     /locus_tag="VVE42_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU3074096.1"
                     /translation="ELIVPMTYEETMTSDFFEAWFQKFFLPTLTTPSVIIMDNARFHR
                     MGKLELLCEEFGHKLLPLPPYSPEYNPIEKTWAHIKKHLKKVLPSCNTFYEALLSFSC
                     FN"
     gene            complement(309..476)
                     /locus_tag="VVE42_001959"
     CDS             complement(309..476)
                     /locus_tag="VVE42_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteinase"
                     /protein_id="HEU3074097.1"
                     /translation="MKKRILSAGLTFASALLLAACDQSGSDTKTYSSTFSGNPTTFNY
                     LLDYYADNIVN"
     gene            complement(641..2248)
                     /locus_tag="VVE42_001960"
     CDS             complement(641..2248)
                     /locus_tag="VVE42_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEU3074098.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNGDGVGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIAAIFGTMEDMDQLIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENTDSPERDYYIWRDEPNDLDSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGDKDLLTVGETWGATPEIAKLYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLDQVEDIESLNYAREALEKGVPIEEIMDS
                     IRVIGRDNARTPMQWDESKNAGFSTDQPWLAVNPNYEMINVQEALANPDSIFYTYQKL
                     VQIRKENSWLIRADFELLDTADKVFAYIRKDGDRRFLVVANLSNEEQDLTVEGKVKSV
                     LIENTLAQEVFEKQILVPWDAFCVELL"
BASE COUNT          638 a          491 c          420 g          714 t
ORIGIN      
        1 tgagttaatc gttccaatga cttacgaaga gacgatgacg agcgactttt ttgaagcttg
       61 gtttcagaag tttttcttac caacattaac cacaccatcg gttattatta tggataatgc
      121 aagattccat agaatgggga agctagaact tttatgcgaa gagtttgggc ataaactttt
      181 acctcttcct ccctactcac ctgagtacaa tcctattgag aaaacatggg ctcatatcaa
      241 aaagcacctc aaaaaggtat taccaagttg caataccttt tacgaggctc ttttgtcttt
      301 ctcttgtttc aattgactat attatcagcg taatagtcta atagatagtt aaatgtagtt
      361 ggatttccac taaaggttga tgagtaagtt tttgtatctg aacctgattg gtcgcaagca
      421 gctaaaagca aagcagatgc aaaagtcagg cctgcactaa ggatacgctt ctttatgttc
      481 gtcttctttc tccttaatag tgggaatttg taaagttaat tgaatttcga gaatgaaagt
      541 ttataaacct tggttataaa aaacaaagga tttccgcctt ttatacagtc ctcccttgtt
      601 tttatatgat ttcaatttta aatttttctg caaaaaatat ttatagtaat tccacacaga
      661 aagcatccca tggaactaag atttgttttt caaagacttc ttgagctagg gtgttttcaa
      721 tcaagacaga tttgactttt ccttctactg tcaagtcttg ctcttcattg gacaagttag
      781 ccacaactag gaagcgacgg tcgccatcct tacgtatata agcaaagacc ttatcagccg
      841 tatcaagcaa ttcaaagtca gctcgaatta gccaactatt ctccttgcga atttggacca
      901 gtttctgata ggtatagaaa atagaatctg gatttgccag cgcttcttgg acgttgatca
      961 tctcgtaatt tggattaact gccaaccaag gttgatctgt tgagaaacca gcgtttttgc
     1021 tctcgtccca ttgcataggg gtacgggcat tgtcacgtcc aataacacgg atactgtcca
     1081 tgatttcttc aatcggaaca cctttttcaa gagcctcacg cgcatagttg agagattcaa
     1141 tatcttctac ttgatccagt gtttcaaacg gatagttggt catcccaatc tcctcacctt
     1201 ggtagatata aggagttcct ctcataagat gaagcaagat tgcaaaggct ttggcagatt
     1261 tttcgcggta ttcttggtca tttccccaga ttgagacgat acgagggagg tcatgattgt
     1321 tccagaagag ggaattccaa ccgtcctcaa ctcctaactc tgtctgccat ttgttgaaga
     1381 tttcttttaa cttagcgata ttcagctctt tttgatagtg ccatttaggc tgaccttcct
     1441 gatactgaag accgatatgt tcaaactgga agaccataga caattcttgc ccctttggat
     1501 cagagtaaag cttggcaatc tctggcgttg ctccccaagt ctcccctact gtcaagagat
     1561 ccttatctcc aaaggtcgcc tgattcattt ccttgagata gggatggagc ataggaccat
     1621 tattgactac cttctcgtca ggaattttgc caatcatgtc aataacatcc atacggaaac
     1681 caccaatacc tttatcaatc cagaagttca tcatctcata aattttctgg cgaagttttt
     1741 cattttccca gttgagatcc ggctgtttct tgctgaaaaa gtggagatag tattgacctg
     1801 acttttcatc gtattcccaa gcagacccac taaagataga atctaggtca ttgggttcat
     1861 cgcgccagat atagtagtct cgctcagggc tgtcagtatt ttcacaggct tcgacaaacc
     1921 aagcatgttc atctgaggta tgattaacca ccaagtccat gatgatacga atgtcacgct
     1981 tcttagcttc tgcaatcagc tgatccatgt cctccatggt tccaaaaata gccgcaatcg
     2041 cttgataatc agcaatatca tagccattat catccatagg gctgtcataa acgggagaaa
     2101 gccaaattgc tgtgattcct agcttggcta gatagtccaa cttactggta atacctggca
     2161 aatcaccaac tccatctcca ttactatcca taaaactctt tggatagact tgatagacta
     2221 cggcattgtg ccaccatttt tcttgcatct gtttacctca tta
//
LOCUS       DAVGJL010000169         2236 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.170, whole genome shotgun sequence.
ACCESSION   DAVGJL010000169 DAVGJL010000000
VERSION     DAVGJL010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.170"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..724
                     /locus_tag="VVE42_001961"
     CDS             <1..724
                     /locus_tag="VVE42_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU3074099.1"
                     /translation="YNDKAVIENLSFNLEEGKSYALVGSSGSGKSTVAKLISGFYNVN
                     KGSIKIGGIAISEYSDEALIKAISFVFQDSKLFKKSIYDNVALANKDATKDDVMRALK
                     LAGCDLILDKFPERENTIIGSKGVYLSGGEKQRIAIARAILKDSKIIIMDEASASIDP
                     DNEFELQKAFKNLMKDKTVIMIAHRLSTIKDLDEIIVMDSGKIIERGSDKELMSKDTR
                     YKSLQEMFNSANEWRVSNERVL"
     gene            708..>2236
                     /locus_tag="VVE42_001962"
     CDS             708..>2236
                     /locus_tag="VVE42_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3074100.1"
                     /translation="MKEFYKKRFALTDGGARNLSKATLASFFVYCINMLPAILLMIFA
                     QEVLENMGKSNGFYIVFSVLTLIAMYILLSIEYDKLYNTTYQESADLRIRTAENLSKL
                     PLSYFSKHDISDISQTIMADIEGIEHAMSHSIPKVGGMVLFFPLISVMMLAGNVKMGL
                     AVIIPSILSFIFIPLSKKYQVNGQNRYYDVLRKNSESFQENIEMQMEIKAYNLSKDIK
                     DDLYKKMEDSERIHLKAEVTTILTLSISSIFSFISLAVVIFVGVNLIINKEINSLYLI
                     GYLLAAMKIKDSLDASKEGLMEIFYLSPKIERLKEIQNQDLQEGDDYSLKKFDIDLKD
                     VEFAYNKDAKVLNGVSFKAKQGEVTALVGASGCGKTTILKLISRLYDYDKGQILIDGK
                     DIKEISTESLFDKVSIVFQDVVLFNQSVMENIRIGKQDASDEEVKRAAKLANCTDFIE
                     KMDKGFDTVIGENGAELSGGERQRLSIARAFLKDAPILILDEITASLDVNNEKKIQES
                     L"
BASE COUNT          846 a          298 c          422 g          670 t
ORIGIN      
        1 ttataatgat aaagctgtca ttgaaaattt atcctttaat ttagaagaag gaaagtccta
       61 cgcacttgtc ggttcatctg gatcaggcaa atcaacagta gcaaaactta tatcaggttt
      121 ttataatgtt aataaaggaa gcataaagat aggcgggata gcaataagtg aatattctga
      181 cgaagcctta attaaagcca tttcctttgt ttttcaagat tcaaaattat tcaagaagag
      241 catttatgat aatgtagctt tagctaataa agatgcgacg aaagatgacg ttatgagagc
      301 cttaaaatta gcaggatgcg atttaatatt agacaaattc ccagaaagag aaaatacaat
      361 cataggctca aaaggtgttt atttatccgg tggagaaaaa caaagaattg caattgctag
      421 agcaatttta aaggattcca aaattattat tatggatgaa gcatcagcat ctattgaccc
      481 agataacgag tttgaattgc aaaaagcttt taaaaatctt atgaaggata aaacagttat
      541 catgattgca cacaggctat ctacaattaa agaccttgat gaaattattg tcatggatag
      601 tggaaaaatt atagaaagag gatctgacaa agaattaatg tcaaaagata caaggtataa
      661 gagcctgcaa gagatgttta acagtgcgaa tgaatggagg gtttcaaatg aaagagtttt
      721 ataaaaaaag atttgctctt acagatggag gagcaagaaa tttaagtaaa gcaacactgg
      781 cttcattttt cgtttattgt ataaacatgc ttcctgccat attacttatg atttttgctc
      841 aggaagtttt ggaaaatatg ggcaaaagca atggctttta tatagtattc tcagttttga
      901 ctttgatagc aatgtatatt ttgctttcta tcgaatacga taaattatat aacacaacct
      961 atcaagaaag tgcagattta agaataagga cagcggagaa tttatcaaaa ttacctctat
     1021 cttacttttc taaacatgac atttccgaca tttcacaaac aatcatggct gatattgaag
     1081 gcatagagca tgcaatgagc cactcaatac caaaggtggg cggcatggta ctgtttttcc
     1141 cattaatatc tgtaatgatg ctagcgggca atgtcaagat gggtttagct gtaattattc
     1201 catctatttt aagctttata tttatacctt tatctaaaaa atatcaggtt aatggacaga
     1261 atagatatta tgatgtctta agaaaaaact cagaaagctt tcaagaaaat atcgaaatgc
     1321 aaatggagat taaagcatat aatttatcga aggatattaa agacgactta tataaaaaaa
     1381 tggaagatag tgagagaata cacttaaagg cggaagtaac tacaatttta actttgtcta
     1441 tatcttcaat atttagcttt atatctcttg ctgttgtgat atttgtcggc gtaaatctaa
     1501 ttattaataa agagataaat tctctctacc ttataggata tttactagct gctatgaaga
     1561 taaaagactc tttagatgca tctaaagagg gcttgatgga aatattttat ttatcgccca
     1621 aaatagaaag attaaaagaa attcaaaatc aagatttaca agaaggcgat gactatagct
     1681 taaaaaaatt tgatattgat ctaaaagatg ttgagtttgc ctacaataaa gacgcaaaag
     1741 ttttaaatgg tgtaagtttt aaagctaagc agggagaggt cactgctttg gtaggtgcaa
     1801 gtggctgcgg taaaacaact atcttgaaac ttatatcaag actttatgat tatgacaagg
     1861 gacaaatctt aatcgatggc aaagatataa aagaaatatc aacagaatct ctttttgata
     1921 aggtctctat agttttccaa gatgtggttc tctttaatca aagcgttatg gaaaatatta
     1981 gaatcggaaa gcaagatgca agtgacgaag aggttaaaag agcagcaaaa cttgcaaatt
     2041 gcacagattt tatagaaaaa atggataaag gtttcgatac agttattggt gagaacggag
     2101 ctgagctatc aggaggagaa agacaaagat tatcaatagc cagagccttc ttaaaagatg
     2161 cgccgatatt gatcttagat gagataacag caagccttga tgttaacaac gagaaaaaga
     2221 ttcaagagtc tttaaa
//
LOCUS       DAVGJL010000170         2225 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.171, whole genome shotgun sequence.
ACCESSION   DAVGJL010000170 DAVGJL010000000
VERSION     DAVGJL010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.171"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(58..1791)
                     /gene="ptsP"
                     /locus_tag="VVE42_001963"
     CDS             complement(58..1791)
                     /gene="ptsP"
                     /locus_tag="VVE42_001963"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEU3074101.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKAAGEAYAKQKSEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            complement(1797..2060)
                     /locus_tag="VVE42_001964"
     CDS             complement(1797..2060)
                     /locus_tag="VVE42_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEU3074102.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
BASE COUNT          581 a          493 c          440 g          711 t
ORIGIN      
        1 aaattctttt taaaatatca aaaaaatccc tgtaactgag ttgcagggac ttttcgatta
       61 atcaaaatta acgtattctt tttgaagttc aagaacttct tccattgttg agcattctgt
      121 aagggcacgg tttgcgtact cttccatctt agctgtgtcg agtttcttca tcaagctgcg
      181 tgtacgaagt acagatgttg ctgacataga gaactcatcc aagcccattc cgacaagaag
      241 tggaacagct tgttggtcac cagccatctc accacacata ccagcccatt taccttcagc
      301 gtgagctgct ttgatcacat tgttaatcaa gcgtaggatt gatgggttgt atggttggta
      361 aaggtatgaa acttgttcgt tcatacggtc tgctgccatt gtatattgga tcaagtcgtt
      421 tgtaccaatt gagaagaagt caacttcttt agcaaattgg tctgcaagca tagccgctgc
      481 aggaatctcg atcatgatac caacttggat gttatccgca actgcaacac cttcagcaag
      541 aaggtttgct ttttcttcat caaagactgc tttcgctgca cggaattctt tcaagagcgc
      601 aaccattggg aacatgatac gcaattgacc gtgaacagac gcacgaagaa gagcacggat
      661 ttgtgtgcgg aacatagcat ctccagtctc agagatagag atacgaagag cacggaatcc
      721 aaggaatggg ttcatttcgt gaggcatatc gaagtaagga agttccttat ctccaccgat
      781 atccattgta cgaacgacaa caggtttacc gttcattcct tcaagaacag ccttgtatgc
      841 ttcatactgc tcatcttcag ttgggaagtc ttgagaatcc atgtacaaga actctgtacg
      901 gtaaagtcca acagcttctg caccgttgtt gttaacacct tcaacgtctt ttggagtacc
      961 aatattggca gccaactcga agtgtttacc gtcagcagtc actgtttgag catctttcaa
     1021 aagtgcccat tcagattttt gtttcgcata ggcttcacca gctgctttaa attctgccgc
     1081 ttgttcatct gttgggttga taatcacttc tccagtgatc ccgttaacag caaggatgtc
     1141 accgtcttta acgatttcag tgatgttatt tgtacctaat acagcagcaa tttcaagtgt
     1201 acgtgccatg atagctgagt ggcttgtacg tccaccaatg ttggttacaa aagcttttac
     1261 aaagtttttg tccaattgag ctgtatcaga tggtgtcaag tcatgcgcaa tcacaatcac
     1321 ttcttcattg atagaagctg ggtttggcaa ttttttacca agaaggtttg ccaatacacg
     1381 ttttgtcacg tcgcggatat ccgctgcgcg ttcttgcatg tatgggttgt cttccatgcc
     1441 ttcaaagata gtgataaaca tatctgtaac ttctttcaga cctgcttctg cattcacttt
     1501 cttcgcacgg atagtttcct tgatttggct gatcatttct gggtcagcaa gaaccattaa
     1561 gtgagcatca aaaacttgag ctgcttcttc accgagcgta cctactgctt tctcgcgaat
     1621 aacagaaagc tcgtcttgtg atgcctgtag agcggcatca aggcgagctt cttctgcgtt
     1681 tgtatcttcg actgtaatag tctcaaatga caaatccggc tgaacgagta gatatgcttt
     1741 tgcaactgca acaccgtcag atgctgcgat tcctttaagc atttctgtca tttcccttat
     1801 gccaatcctt ctttttccat tgtttctgag attgcagcga tagcgtcatc tgcatctgca
     1861 ccttcagctg agatagttac gtcagcacct tggccaacac caagactcat aacacccata
     1921 attgatttaa ggttaactga tttacctttg tactcaagag tgatatctga agcaaatttg
     1981 ctagcagttt gtaccaacaa tgttgctgga cgtgcgtgaa tacctgtttc tgccactacg
     2041 tggaaatctt tagaagccat agtttgactc tccttttgtt ctttcttttt ttgagttata
     2101 tgtgataacc cttacaattt agtattatac catcttctag gatttttttc aagcattttc
     2161 tggattttcc ttcaatttcc tttcagataa cttgctgaaa atcttctatc ctagagaaca
     2221 aaaca
//
LOCUS       DAVGJL010000171         2190 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.172, whole genome shotgun sequence.
ACCESSION   DAVGJL010000171 DAVGJL010000000
VERSION     DAVGJL010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.172"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            85..2022
                     /gene="pspA"
                     /locus_tag="VVE42_001965"
     CDS             85..2022
                     /gene="pspA"
                     /locus_tag="VVE42_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein A"
                     /protein_id="HEU3074103.1"
                     /translation="MNKKKMILTSLASVAILGAGFVTSQPTVVRAEESPQVVEKSSLE
                     KKYEEAKAKADTAKKDYETAKKKAEDAQKKYEDDQKRTEEKARKEAEASQKLNDVALV
                     VQNAYKEYREVQNQRSKYKSDAEYQKKLTEVDSKIEKARKEQQDLQNKFNEVRAVVVP
                     EPNALAETKKKAEEAKAEEKVAKRKYDYATLKVALAKKEVEAKELEIEKLQYEISTLE
                     QEVATAQHQVDNLKKLLAGADPDDGTEVIEAKLKKGEAELNAKQAELAKKQTELEKLL
                     DSLDPEGKTQDELDKEAEEAELDKKADELQNKVADLEKEISNLEILLGGADSEDDTAA
                     LQNKLATKKAELEKTQKELDAALNELGPDGDEEETPAPAPQPEQPAPAPKPEQPAPAP
                     KPEQPAPAPKPEQPAKPEKPAEEPTQPEKPATPKTGWKQENGMWYFYNTDGSMAIGWL
                     QNNGSWYYLNANGAMATGWVKDGDTWYYLEASGAMKASQWFKVSDKWYYVNSNGAMAT
                     GWLQYNGSWYYLNANGDMATGWLQYNGSWYYLNANGDMATGWLQYNGSWYYLNANGDM
                     ATGWAKVNGSWYYLNANGSMATGWVKDGDTWYYLEASGAMKASQWFKVSDKWYYVNGL
                     GALAVNTTVDGYKVNANGEWV"
BASE COUNT          832 a          375 c          479 g          504 t
ORIGIN      
        1 aaacatttga caaatattta cggggggagt atacttaata taattatagt ctaaaaatga
       61 ctatcagaaa agaggtaaat ttagatgaat aagaaaaaaa tgattttaac aagtctagcc
      121 agcgtcgcta tcttaggggc tggttttgtt acgtctcagc ctactgttgt aagagcagaa
      181 gaatctccac aagttgtcga aaaatcttca ttagagaaga aatatgagga agcaaaagca
      241 aaagctgata ctgccaagaa agattacgaa acggctaaaa agaaagcaga agacgctcag
      301 aaaaagtatg aagatgatca gaagagaact gaggagaaag ctcgaaaaga agcagaagca
      361 tctcaaaaat tgaatgatgt ggcgcttgtt gttcaaaatg catataaaga gtaccgagaa
      421 gttcaaaatc aacgtagtaa atataaatct gacgctgaat atcagaaaaa attaacagag
      481 gtcgactcta aaatagagaa ggctaggaaa gagcaacagg acttgcaaaa taaatttaat
      541 gaagtaagag cagttgtagt tcctgaacca aatgcgttgg ctgagactaa gaaaaaagca
      601 gaagaagcta aagcagaaga aaaagtagct aagagaaaat atgattatgc aactctaaag
      661 gtagcactag cgaagaaaga agtagaggct aaggaacttg aaattgaaaa acttcaatat
      721 gaaatttcta ctttggaaca agaagttgct actgctcaac atcaagtaga taatttgaaa
      781 aaacttcttg ctggtgcgga tcctgatgat ggcacagaag ttatagaagc taaattaaaa
      841 aaaggagaag ctgagctaaa cgctaaacaa gctgagttag caaaaaaaca aacagaactt
      901 gaaaaacttc ttgacagcct tgatcctgaa ggtaagactc aggatgaatt agataaagaa
      961 gcagaagaag ctgagttgga taaaaaagct gatgaacttc aaaataaagt tgctgattta
     1021 gaaaaagaaa ttagtaacct tgaaatatta cttggagggg ctgattctga agatgatact
     1081 gctgctcttc aaaataaatt agctactaaa aaagctgaat tggaaaaaac tcaaaaagaa
     1141 ttagatgcag ctcttaatga gttaggccct gatggagatg aagaagaaac tccagcgccg
     1201 gctcctcaac cagagcaacc agctcctgca ccaaaaccag agcaaccagc tcctgcacca
     1261 aaaccagagc aaccagctcc agctccaaaa ccagagcaac cagctaagcc ggagaaacca
     1321 gctgaagagc ctactcaacc agaaaaacca gccactccaa aaacaggctg gaaacaagaa
     1381 aacggtatgt ggtatttcta caatactgat ggttcaatgg caataggttg gctccaaaac
     1441 aacggttcat ggtactacct aaacgctaac ggcgctatgg caacaggttg ggtgaaagat
     1501 ggagatacct ggtactatct tgaagcatca ggtgctatga aagcaagcca atggttcaaa
     1561 gtatcagata aatggtacta tgtcaacagc aatggcgcta tggcgacagg ctggctccaa
     1621 tacaatggct catggtacta cctcaacgct aatggtgata tggcgacagg ctggctccaa
     1681 tacaacggtt catggtatta cctcaacgct aatggtgata tggcgacagg ctggctccaa
     1741 tacaacggtt catggtatta cctcaacgct aatggtgata tggcgacagg atgggctaaa
     1801 gtcaacggtt catggtacta cctaaacgct aacggttcaa tggcaacagg ttgggtgaaa
     1861 gatggagata cctggtacta tcttgaagca tcaggtgcta tgaaagcaag ccaatggttc
     1921 aaagtatcag ataaatggta ctatgtcaat ggcttaggtg cccttgcagt caacacaact
     1981 gtagatggct ataaagtcaa tgccaatggt gaatgggttt aagccgatta aattaaatca
     2041 tgttaagaac atttgacatt ttaattttga aacaaagata aggttcgatt gaatagattt
     2101 atgttcgtat tctttaggta cctatcttat gatttcagga aatgtcatta aaaaaacgac
     2161 tccttttctc taacttgaaa aatagattag
//
LOCUS       DAVGJL010000172         2190 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.173, whole genome shotgun sequence.
ACCESSION   DAVGJL010000172 DAVGJL010000000
VERSION     DAVGJL010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.173"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(<1..1448)
                     /gene="pfbA"
                     /locus_tag="VVE42_001966"
     CDS             complement(<1..1448)
                     /gene="pfbA"
                     /locus_tag="VVE42_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HEU3074104.1"
                     /translation="MKYFVPNEVFSIRKLKVGTCSVLLAISILGSQGILSDEVVTSSS
                     PMATKESSNAITNDLDNSPTVNQNRSAEMIASNSTTNGLDNSLSVNSISSNGTIRSNS
                     QLDNRTVESTVTSTNENKSYKEDVISDRIIKKEFEDTALSVKDYGAVGDGIHDDRQAI
                     QDAIDAAAQGLGGGNVYFPEGTYLVKEIVFLKSHTHLELNEKATILNGINIKSHPSIV
                     FMTGLFTDDGAQVEWGPTEDISYSGGTIDMNGALNEEGTKAKNLPLINSSGAFAIGNS
                     NNVTIKNVTFKDSYQGHAIQIAGSKNVLVDNSRFLGQALPKTMKDGQIISKESIQIEP
                     LTRKGFPYALNDDGKKSENVTIQNSYFGKSDKSGELVTAIGTHYQTLSTQNPSNIKIL
                     NNHFDNMMYAGVRFTGFTDVLIKGNRFDKKVKGESVHYRESGAVLVNAYSYKNTKDLL
                     DLNKQVVIAENIFNIADPKTKAIRVAKDSAEY"
BASE COUNT          697 a          414 c          298 g          781 t
ORIGIN      
        1 aaatattctg cactatcttt tgcaactcgt atcgcttttg ttttaggatc ggcaatatta
       61 aatatatttt ctgcgataac cacctgttta tttaaatcta ataggtcttt agtgttttta
      121 tagctataag catttactaa aactgctccg ctttctcgat aatgtacact ctctccttta
      181 actttcttat caaagcgatt tcctttgatt aatacatcag tgaatcctgt aaaacgtaca
      241 cctgcataca tcatgttatc aaaatgatta tttaaaattt taatattaga ggggttctgt
      301 gtcgacaatg tttgatagtg tgtgccaatt gctgttacta attccccaga tttatcactt
      361 ttgccaaaat aggaattttg aatagtcaca ttttcagatt ttttcccatc atcattcaag
      421 gcataaggaa aaccttttct agttaatggt tcaatctgaa tgctctcctt acttatgatt
      481 tgcccatcct tcatcgtttt gggtaaggct tgcccaagaa aacgagaatt atcaactaat
      541 acatttttcg aacctgcaat ttgaatagca tgcccttgat aactatcctt gaatgttaca
      601 ttttttatag ttacgttatt tgaattccca atagcaaatg cacctgaaga atttataagt
      661 ggtagatttt ttgctttagt tccttcttca ttcaaagcac cgttcatatc aatcgtacca
      721 ccagaataac taatatcttc tgttgggccc cattctactt gcgcaccatc atccgtaaat
      781 aaacctgtca taaaaacaat ggaaggatga ctcttaatat ttataccatt tagaattgta
      841 gctttctcat tcaattctaa gtgtgtatga ctttttaaaa aaacaatttc ttttactaaa
      901 tacgttcctt caggaaaata tacatttcct ccacctagcc cttgagctgc agcatctatt
      961 gcatcttgaa ttgcttgtcg atcatcatga atcccatcac ctaccgcacc ataatctttt
     1021 acacttaaag cagtatcttc aaattctttt ttgataattc tgtcacttat aacatcttcc
     1081 ttataactct tattttcatt agtagatgtt actgtagatt caactgttct gttgtctaat
     1141 tgtgaattgg aacgaatagt accattagag ctgatactat taacacttaa cgaattatct
     1201 aaaccattag tggttgaatt agaggcaatc atttcagcag aacgattctg attaacagtt
     1261 ggtgaattat ctaaatcatt agtaattgca ttagaagact cttttgtagc catcggtgaa
     1321 gaactagtaa caacttcatc cgataaaata ccttggcttc ccaaaattga aattgccaat
     1381 agtaccgagc aagtccccac ctttaattta cgaatactga atacctcatt aggaacaaaa
     1441 tatttcatca gaacctccaa tttttttact atttttaaca attgattatt acttttctta
     1501 aaatagatag aatgacaccg ctgtcattat ctttatcata tattttccaa attgaattgt
     1561 caaggaataa cacaagaaaa tattaaaatt tatactcttc ggaaatctct tcaaactagg
     1621 tcagtgccgc cttgccctaa gtctaggtta ctgacttggt cagttctata gtaaattgaa
     1681 actaaaatag tacacctcta cttctaaaac attgttagaa atcgatttga ctgtcccgat
     1741 caatttgtcc tattcttatt tcattttact atatctacaa cctcaaagca gggttttgag
     1801 ctatctatgg ccaacttcct agtttgctct ttagccttcc attcaaaagt gatgctatct
     1861 ttcttagctt gaatataaaa tatcccattt actatattta aatttattga tatgttgtaa
     1921 attataaatg aaagaacaaa taccttgtca tacaagcatt cgccagggat atttacatga
     1981 atatcgctca tatggcggat tgaaactaaa acactacaat atatttctta aatagacctt
     2041 ctataaaagc tagatttaac ctcgataaaa atagatgcag tgcttctaaa atactggaat
     2101 agggcatata ggtaatagta gacaagtgtt ttatagattg tctcgcagga gatctaatga
     2161 tgtatagcaa gcatcactaa acatatagaa
//
LOCUS       DAVGJL010000173         2141 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.174, whole genome shotgun sequence.
ACCESSION   DAVGJL010000173 DAVGJL010000000
VERSION     DAVGJL010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.174"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            386..766
                     /locus_tag="VVE42_001967"
     CDS             386..766
                     /locus_tag="VVE42_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0115 family bacteriocin-like peptide"
                     /protein_id="HEU3074105.1"
                     /translation="MELVLPNNYVALEQEEMMYLDGGGVGRNWWNSRGSFATVLDVDL
                     AIYSGGATIYSAYAIKKAISANRGAITRTLRSLIIKHVGSAAGHLVNTALNVALTVTG
                     FSLGGAIAYGVDWADGSLDGYIFA"
     gene            complement(1166..2029)
                     /locus_tag="VVE42_001968"
     CDS             complement(1166..2029)
                     /locus_tag="VVE42_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU3074106.1"
                     /translation="MRYDFGKVYKEIRESKGLTQEEVCGGVLSRTSLSKIESGKTTPK
                     YENMEFLLRQINMSFEEFEYICQLYQPSQRTEIMQTYLNMRSIIGTSDLVNLFQKCQD
                     YLKTHHDLPIEEIRDMLEVVIYIRQHGAGELSDHAEQVVKKLWRKIEKQDTWYESDLK
                     ILNTILFSFPIEYLHLITGKILQRLEVYKNYQHLYGLRIAILLNLSTLYLYNQDKNMC
                     KQICYTLLEDAKNKKSYDRLAICYVRIGICTDDSKLIQKGFSLLELTEETSMLSHLKK
                     EVEIYYQAKER"
BASE COUNT          651 a          298 c          440 g          752 t
ORIGIN      
        1 atggggctgg tcgatataat gtaagggagc ttgagttgtt agtggaggat aaaatgttaa
       61 gagtaatagt agttattcaa tctttagtaa taatactgct tgctgttgct ttattattat
      121 ctcggaaaaa ctttgattcg ttaaatgttg ttcttataat gatgatcgta tcacaattgt
      181 tgttattagt atatttgata aaatcaggga atcagtgatt agctgttatt tgaattaagt
      241 aaactgttaa gcttcaagca gtaactggtt ttaaatattt aaaataagat aaaggaagga
      301 agtctaattt aggtaactaa acaaagcctg cgatcacttg ttaggaaact atcctaacca
      361 tgcttaataa aaaaggagaa aataaatgga actcgtatta ccaaataatt atgttgctct
      421 tgagcaagaa gagatgatgt atcttgatgg gggtggtgtt ggtcgtaact ggtggaatag
      481 tagaggtagt tttgcaacag ttctggatgt agatttggcc atctatagtg gtggtgcaac
      541 aatttattct gcttatgcga taaaaaaagc tatctcagct aatagagggg ctattacgag
      601 aacattacgt agtttaataa ttaaacatgt aggtagtgca gctggccatt tagtcaatac
      661 tgcactaaac gttgcactaa ctgttactgg attttcacta ggtggagcaa tcgcatatgg
      721 ggttgattgg gctgacggta gcttagatgg ttatattttt gcttaaaatg aaacattttt
      781 ttgcaggaat tggtgaaata aactttgtaa gttatttatt gtatatatgt gtaggaatag
      841 cccctttttt tcacgtatat ataataggtt ctgaaatgaa ttttgtgaaa atagtgttat
      901 ctattttggg aattatattt gtttgtattt taactattgc tcgaatttat aggacttttt
      961 tctataaaga atagtttgga cttgaaatgt atttaaaaac aagaggttgg tagattatca
     1021 gcctctttct tgtcgttgaa aaagtgagat atattgactt ttgaaaaaaa tgtcataatt
     1081 gagttgggat aaacgtaaaa gtcatacttt ctcgttaaat cgttcggcga ttctatgaaa
     1141 aggtcgagga gctccctcgg cttttttatc tttccttcgc ttggtaatag atctctactt
     1201 cttttttgag atgagacagc atagaagttt cctcggtcag ctctaggagt gagaaacctt
     1261 tttggataag tttagaatcg tctgtacaaa tcccaatacg gacatagcag atagccagcc
     1321 tatcgtagct tttcttattc ttggcgtcct ccagtaaagt gtagcagatt tgcttacaca
     1381 tgtttttatc ttgattgtat agatagagtg tggaaaggtt gagcaggatt gctattcgca
     1441 agccatataa atgttgatag tttttataga cttctaagcg ttgcaagatt tttccagtga
     1501 tgagatggag gtattcaatg ggaaaactga aaagaatggt attgaggatt tttagatcac
     1561 tctcatacca tgtatcttgt ttttcaattt ttctccaaag ttttttgaca acctgttctg
     1621 cgtgatctga tagttcccct gccccgtgtt gacggatata aatgacaact tcaagcatat
     1681 ctctgatttc ttctataggg agatcgtggt gggtcttgag gtagtcttgg catttttgaa
     1741 ataaattaac aagatcgctg gtcccgataa tggaacgcat attgagatag gtttgcataa
     1801 tctctgtgcg ttgacttggt tgataaagtt gacagatata ttcaaactct tcaaaactca
     1861 tattgatttg acggagaaga aattccatat tttcatattt tggggttgtc ttgccacttt
     1921 cgatttttga taagctggtt cttgagagaa cacccccaca gacctcttct tgggtcagcc
     1981 cctttgactc acgtatttct ttatagacct ttccgaaatc ataacgcatg attttctcct
     2041 ttctgtgaaa aaagttaaca aataataaaa tcttgttaaa aatattgtat catagcaatg
     2101 taaaaagtaa aaaagaaagt cgaaataaat ttgtgaaaaa a
//
LOCUS       DAVGJL010000174         2134 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.175, whole genome shotgun sequence.
ACCESSION   DAVGJL010000174 DAVGJL010000000
VERSION     DAVGJL010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.175"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(273..569)
                     /locus_tag="VVE42_001969"
     CDS             complement(273..569)
                     /locus_tag="VVE42_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uberolysin/carnocyclin family circular
                     bacteriocin"
                     /protein_id="HEU3074107.1"
                     /translation="MKSKRMEFHNKFLIVSAMLAVISWLSLGVVSFPMLAGTLGISTK
                     AAATVVNLISAYSTVTAVISIVGAITGVGSIGSGIAATVLYILKKKGAAKAALW"
     gene            complement(1099..1992)
                     /locus_tag="VVE42_001970"
     CDS             complement(1099..1992)
                     /locus_tag="VVE42_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU3074108.1"
                     /translation="MDELKREIGYKIKTIRRSCGKTQIDICGDESELTIRQLARIENG
                     QALATLPKLLLIADKLGVSLQNIVDVKVIEIPKGFLKLKDELIHSQTYADKGRIERKE
                     AILEEIYENYYENLPEEEQLIVDVTQARFDIYGSSDVTYGLGLVEEYFQQLLKKKYFS
                     VNDLLIIELYFFCCAMGLEDKEHFEELAQKVLLCSEYEDKASLVQMEKVLLILFIQIQ
                     TEDSLIYIQTFEKIIAKTRHVFYRPHLFLLKAKYALFVDKNILEAESFYEKAISLAEL
                     LDDQVLVQRILAEKQIDFPTT"
BASE COUNT          736 a          409 c          247 g          742 t
ORIGIN      
        1 acaatggcaa gaagatagag aaaaatatag tttttcctta tatagaaagt atttattttt
       61 ttactttgga taggcagttt catattataa cgattcaaag tagcgtaatc tactaccaaa
      121 aaaagtaaaa ataatttccc ttgaaaagga agatagcttt taaaatagat tgacaaactt
      181 atgataacaa aaaacaaaag ggttatccca ataaaattaa gataacctaa aatactttca
      241 cattcttttt tagaaagtat tttcatctag ttctaccata gagctgcttt agcagcacct
      301 ttctttttca atatataaag aactgttgct gcaatacccg aaccaataga acctactcct
      361 gtgattgcac ctacaattga aattactgct gtaactgtac tataagcact aatcaaattg
      421 actacagtag cagcagcttt tgtcgaaatt cctaatgtcc cagccaacat aggaaaagaa
      481 acaacaccta agcttaacca actaatgact gctaacattg ctgaaacaat aagaaattta
      541 ttatgaaatt ccattctttt agatttcatt ttagtgtact ccttttgtat attatgtatg
      601 ttttattgag agttaattcg cacatgttgt attcctaact ctcttcaaat gttcgtctat
      661 attttatact acttttatta acatatctat aataaataaa ttactactac attttcctag
      721 attatcaaat tacttttata tcactaagac tcaataaaag ataatgtcta tttaactttt
      781 atacgattac tacctctttt tagtttacac ttcctacatc ataaataaac atactcaacc
      841 ctcacaatct ccaaataact cttctatatc attgaaaatt gagtttcatc ctctatacta
      901 atgtaggaca ttcctttaat ccaatataag aataccttgt tccattaatc tctacccgat
      961 attatagata gaaccaaatt cctatactta aaaggacaaa taacgttcca accataataa
     1021 acctcctttc tttttgagaa cgttttcttt ataggtttag tatagcaaaa acatgaaaaa
     1081 aacaacatga cttagaagtc aggttgttgg gaaatctatc tgtttttctg ctaaaattct
     1141 ctgaactaat acctgatcgt ctagcaattc tgctagagaa atcgcctttt catagaaact
     1201 ttctgcctct aaaatattct tgtctacaaa taaagcatat tttgccttta gcaaaaatag
     1261 atgaggtcgg taaaaaacat gtcttgtttt agcaataatt ttctcaaatg tttgaatata
     1321 tatcagacta tcctctgtct gaatttggat aaataggatc agaagaactt tttccatttg
     1381 aactaggcta gccttatctt catattctga acatagcaat actttttgag ctaactcctc
     1441 aaaatgttct ttatcttcta atcccatagc acaacaaaag aaatacaatt caataatcaa
     1501 caaatcattt acagaaaaat atttcttttt taataactgc tggaaatatt cttccactaa
     1561 ccctaaaccg taggtcacat ctgaactacc ataaatatca aaacgagctt gtgtcacatc
     1621 cacaattaac tgctcttcct caggcaaatt ctcataatag ttttcatata tttcctcaag
     1681 tatagcttct tttcgttcga ttcttccctt atcagcgtag gtttgagaat gaatcagctc
     1741 atctttgagt tttaggaatc cttttggaat ctctataacc ttaacatcca caatattctg
     1801 taatgatact ccaagtttat ccgcaatcaa taataattta ggaagtgttg ctaaagcttg
     1861 cccattttca atacgtgcta gctgacgaat cgtcaactct gattcatcac cacaaatatc
     1921 tatctgagtt tttccacaag atcgcctaat agttttgatt ttataaccaa tttcacgttt
     1981 caactcatcc atagtaatac tccttaagtt gattacaaag atttcttttt cttcctaatt
     2041 cttttgtcaa tcatatataa aacgataagc gaaaacagcg gtagaacaat ataactcttt
     2101 tcactcatta tcagctgaaa tcccagggtt gcta
//
LOCUS       DAVGJL010000175         2046 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.176, whole genome shotgun sequence.
ACCESSION   DAVGJL010000175 DAVGJL010000000
VERSION     DAVGJL010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.176"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(1..408)
                     /locus_tag="VVE42_001971"
                     /pseudo
     CDS             complement(1..408)
                     /locus_tag="VVE42_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(353..535)
                     /locus_tag="VVE42_001972"
     CDS             complement(353..535)
                     /locus_tag="VVE42_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU3074109.1"
                     /translation="MDLKFEGVDLEYKKAKNNLPESFWETYSAFANTNGGKIILGIDE
                     KNIDTYQRVNRLPAKL"
     gene            804..>2046
                     /gene="pcpA"
                     /locus_tag="VVE42_001973"
     CDS             804..>2046
                     /gene="pcpA"
                     /locus_tag="VVE42_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein PcpA"
                     /protein_id="HEU3074110.1"
                     /translation="MKKTTILSLTTAAVILAAYVPNEPILADTPSSEVIKETKVGSII
                     QQNNIKYKVLTVEGNIGTVQVGNGVTPVEFEAGQDGKPFTIPTKITVGDKVFTVTEVA
                     SQAFSYYPDETGRIVYYPSSITIPSSIKKIQKKGFHGSKAKTIIFDKGSQLEKIEDRA
                     FDFSELEEIELPASLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFANLSNLEKLT
                     LPKSVKTLGSNLFRLTTSLKHVDVEEGNESFASVDGVLFSKDKTQLIYYPSQKNDESY
                     KTPKETKELASYSFNKNSYLKKLELNEGLEKIGTFAFADAIKLEEISLPNSLETIERL
                     AFYGNLELKELILPDNVKNFGKHVMNGLPKLKSLTIGNNINSLPSFFLSGVLDSLKEI
                     HIKNKSTEFSVKKDTFAIPETV"
BASE COUNT          708 a          314 c          340 g          684 t
ORIGIN      
        1 attgatttgt acttccgtcg catcaatcat taccgtgtcc tcagagctga gaggagttct
       61 tgaaatcgta acaccacttt gaacaagagt tacttcaacc cattggctcc gacggagtaa
      121 gttgctttcg tggataccaa aatcagccgc aatttcttca taagtgcggt attctcacac
      181 atattgaaga gtgcccataa gaaggtcttc taggcttaat ttaggttttc gtccaccttt
      241 tgcgtgttta agttgataag ctgtttttaa tacagctaac atctctttaa aagtcgtgcg
      301 ctgaacacca acaagacgct taaatcgtgt atcagttaat tgtttacttg cttcataatt
      361 tcgcagggag tctattgact ctttggtagg tgtcaatgtt tttttcatct atcccgagaa
      421 ttattttccc gccatttgta tttgcaaatg ctgagtaggt ttcccagaaa gactctggaa
      481 gattgttttt agcttttttg tattctaaat caaccccttc aaattttaag tccatatttt
      541 tcctttgcat ctgttttttg tggttctggt atttgttcaa gttgagtgat aatatagcga
      601 attgaatttc gagagttttt actcagttaa ttttccttga cttaaaattt aaaaatgata
      661 tactgtgttt atggagagat gacaatttat aaaatgtact actctattct actagataat
      721 gattgaataa aactttttat aacaacggct agcaaagtta taattttata tctattagaa
      781 aataaatatg taaggaaatt taaatgaaaa aaactacaat attatcatta actacagctg
      841 cggttatttt agcagcatat gtccctaatg aaccaatcct agcagatact cctagttcgg
      901 aagtaatcaa agagactaaa gttggaagta ttattcaaca aaataatatc aaatataagg
      961 ttctaactgt agaaggtaac ataggaactg ttcaagtggg taatggagtt actcctgtag
     1021 agtttgaagc tggtcaagat ggaaaaccat tcacgattcc tacaaaaatc acagtaggtg
     1081 ataaagtatt taccgttact gaagtagcta gtcaagcttt tagttattat ccagatgaaa
     1141 caggtagaat tgtctactat cctagctcta ttactatccc atcaagcata aaaaaaatac
     1201 aaaaaaaagg cttccatgga agtaaagcta aaactattat ttttgacaaa ggcagtcagc
     1261 tggagaaaat tgaagataga gcttttgatt tttctgaatt agaagagatt gaattgcctg
     1321 catctctaga atatattgga acaagtgcat tttcttttag tcaaaaattg aaaaagctaa
     1381 ccttttcctc aagttcaaaa ttagaattaa tatcacatga ggcttttgct aatttatcaa
     1441 atttagagaa actaacatta ccaaaatcgg ttaaaacatt aggaagtaat ctatttagac
     1501 tcactactag cttaaaacat gttgatgttg aagaaggaaa tgaatcgttt gcctcagttg
     1561 atggtgtttt gttttcaaaa gataaaactc aattaattta ttatccaagt caaaaaaatg
     1621 acgaaagtta taaaacgcct aaggagacaa aagaacttgc atcatattcg tttaataaaa
     1681 attcttactt gaaaaaactc gaattgaatg aaggtttaga aaaaatcggt acttttgcat
     1741 ttgcggatgc gattaaactt gaagaaatta gcttaccaaa tagtttagaa actattgaac
     1801 gtttagcctt ttacggtaat ttagaattaa aagaacttat attaccagat aatgttaaaa
     1861 attttggtaa acacgttatg aacggtttac caaaattaaa aagtttaaca attggtaata
     1921 atatcaactc attgccgtcc ttcttcctaa gtggcgtctt agattcatta aaggaaattc
     1981 atattaagaa taaaagtaca gagttttctg tgaaaaaaga tacatttgca attcctgaaa
     2041 ctgtta
//
LOCUS       DAVGJL010000176         1980 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.177, whole genome shotgun sequence.
ACCESSION   DAVGJL010000176 DAVGJL010000000
VERSION     DAVGJL010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.177"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(<1..226)
                     /locus_tag="VVE42_001974"
     CDS             complement(<1..226)
                     /locus_tag="VVE42_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3074111.1"
                     /translation="MTTKKANITIEEYIEMSEVDFNEAVNYEFTSDTCQLANSIYQSL
                     FKFFDKKNFSGDLIFTWKSPSLVKEGDYIGR"
     gene            complement(255..953)
                     /locus_tag="VVE42_001975"
     CDS             complement(255..953)
                     /locus_tag="VVE42_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEU3074112.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFSHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIERDYNLYAATNGITAIQTGRLAQSGLVPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNIL"
     gene            complement(1412..1792)
                     /locus_tag="VVE42_001976"
     CDS             complement(1412..1792)
                     /locus_tag="VVE42_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEU3074113.1"
                     /translation="MESIGIGLVIVSHSKHIAEGVVELISKVAKDVPITYVGGTEGGG
                     IGTSFDQVDRVVSENPADTLLAFFDLGSAKMNLKMVTDFSDKSIIINRVPIVEGAYNA
                     AALLQAGAELSVIQIQLAELEINK"
BASE COUNT          657 a          389 c          309 g          625 t
ORIGIN      
        1 ttctcccaat ataatcccct tctttgacta atgatggaga tttccaagta aaaattaaat
       61 cgccagagaa atttttctta tcaaaaaact taaaaagaga ttgataaata ctatttgcta
      121 attgacaagt gtcagatgta aattcataat taacagcttc attaaaatca acttcagaca
      181 tttcaatgta ctcttcaata gtaatattag ctttttttgt agtcattagc taatctcctt
      241 taaaatgtga tcttttaaag aatattttta tctaaacaat ccagcaagtc ttggtaagaa
      301 tagacttcgt aagtcggctg ggcttgtgtg tgattttcga ggtgatgagg attataccag
      361 atagtgtcaa tccccgcatt attgccacct tgaatgtcgg cggttagaga atctccaatc
      421 atcagcgtct tttctttact aaatccagca atttgctggc caatcttttc ataaaagaga
      481 gcatccggct tttgagtttg caactgttct gagataaaga cttgattgaa ataaggtact
      541 agaccagatt gagccaaacg tcctgtctga atggcagtaa tgccatttgt cgcagcatac
      601 aagttataat cacgctcaat gaggctgtcc aagagatcat gagcgcccga tagtgtttgt
      661 ccctgctgag cgagataaaa ttggtaacgc tgggcaagaa aactaccgtc tttttcctgt
      721 ccaaaatgag aaaataaacg agaaaagcgc gtgttaacca gctcttgttt actgattttc
      781 ttcagctcca agtctttcca gagagccttg ttcataggaa cgtaataatc tttataagcc
      841 tgaatatccg caactccttc ttcttttaga agttgggtca aagccacatc ctcagcagca
      901 tcaaaatcaa gaagagtgtg gtcgaggtcg aagagtagaa atttgtagaa caatttgagg
      961 ttttcctttc tgaaaattca ttaagaacat tatatcataa agcacctcat acaattaact
     1021 aatttaatca cttaaaaaaa attcgaacac tttctataca actgacagct caaatctttc
     1081 agaatagaac aatactaact atcgagcacc ccgtcttcat aaatatatgt gtaattctag
     1141 acctagaatt cctataaact aaatgctttc atactcttcc aagtaattga ttgccttaaa
     1201 ttttaatttt tgaaggtttc taaagctaga atagccccat cacaatcagt tttgattgat
     1261 tcacaattta gaaacactat agtttcactc ctgttaaaat aaaaaggaac tgcataaagc
     1321 aatccctttc tgattttgaa atcatttact taacatttta tagttgagat aatcaatagc
     1381 ttatctataa aaagagttat agtaaaattc cttatttatt gatttcaagc tccgctaact
     1441 gtatttgaat aactgacagt tctgcaccag cctgaagaag agcagctgca ttataggcac
     1501 cttctacaat tggaaccctg ttgatgatga tacttttatc actgaaatca gtcaccattt
     1561 ttaagttcat tttagcagaa cctaggtcaa aaaaggcaag taaagtatct gctggatttt
     1621 cggaaacaac cctatctact tgatcaaaac tcgttccaat tcctccgccc tcggttcctc
     1681 ctacataagt aatcggaaca tctttagcta ctttactaat cagttcaaca acaccttctg
     1741 caatgtgttt ggaatgtgaa acgataacaa gaccaatacc aatactttcc atcaaaccac
     1801 tccagtttct aaaatagcag taaagagtaa tcctgatgag aatgatccag gatcaatatg
     1861 tccaagaaac cacatgctcc taagacaaga gctaacagac tggccatcaa taatagtatt
     1921 gttctttttt tcatcattac tccttaacta gtgtttaact gattaattag ccagtaaata
//
LOCUS       DAVGJL010000177         1932 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.178, whole genome shotgun sequence.
ACCESSION   DAVGJL010000177 DAVGJL010000000
VERSION     DAVGJL010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.178"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            60..1550
                     /locus_tag="VVE42_001977"
     CDS             60..1550
                     /locus_tag="VVE42_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3074114.1"
                     /translation="MNLERFLKLSIYQMIVWCGIIFLDWVVKNYQVEVIQEFNLEIRN
                     RVATDISNSTYQEFHSKSSGTYLSWLNNDVQTLNDQAFKQLFLVIKGISGTIFAVVTL
                     NHYHWSLTVATLFSLMIMLLVPKIFASKMREVSLNLTNQNEAFLKSSETILNGFDVLA
                     SLNLLYVLPKKIKEAGILLKMVIQRKTTVETLAGAISFFLNIFFQISLVFLTGYLAIK
                     GIVKIGTIEAIGALTGVIFTALGELGGQLSSIIGTKPIFLKLYSINPIESNKMNDIEP
                     NEVNRDFPLYEAKNICYKYGDKEILKNLNFCFQRNEKYLILGESGSGKSTLLKLLNGF
                     LRDYSGELRFCGDDIKKTSYLNMVSNVLYVDQKAYLFEGTIRDNILLEENYTDEEILQ
                     SLEQVGLSVKDFPNNILDYYVGDDGRLLSGGQKQKITLARGLIRNKKIVLIDEGTSAI
                     DRRTSLAIERKILDREDLTVIIVTHAPHPELKQYFTKIYQFPKDFI"
BASE COUNT          676 a          237 c          359 g          660 t
ORIGIN      
        1 aattattgct aatttatgca gcaactatta atgctctggt gttgaatgaa ttaattgcga
       61 tgaatttaga gcggtttttg aaattgtcaa tctaccaaat gattgtctgg tgtgggataa
      121 tattccttga ctgggtagtg aaaaattatc aggttgaagt gatccaagag tttaatctag
      181 agattcgaaa tagagttgcc acagacatct ctaactctac ctatcaagaa tttcatagta
      241 aatcatcagg aacatatctt tcgtggctaa ataatgatgt tcagacttta aatgatcagg
      301 cgtttaaaca acttttttta gtaataaaag gaatttctgg tactatattt gcagttgtga
      361 ctcttaatca ctatcattgg tcattgactg tagccacctt gttttcatta atgattatgc
      421 tacttgtacc aaaaatcttt gcatcgaaaa tgcgagaagt tagtctaaat ttaactaacc
      481 aaaatgaagc ttttttaaaa tctagtgaga ctatattgaa tggatttgat gtgttagcgt
      541 ccttgaatct tttatatgta ttgcctaaga aaattaaaga agcaggaatt ttattaaaga
      601 tggttataca aagaaagaca actgtagaaa cgttagcagg cgctattagc ttctttctca
      661 atattttttt tcagatatct ctcgtttttt taacaggcta tcttgcaata aaaggaatag
      721 tgaaaattgg tactattgaa gcaataggag cactaacagg tgttattttt acagcgctag
      781 gtgaattagg aggtcaatta tcctctatta ttggtacgaa gcctattttt ttaaaattgt
      841 attcaattaa tccaattgag tcaaataaaa tgaatgatat cgaaccaaat gaggtgaata
      901 gagattttcc gttatatgaa gcaaaaaata tttgctataa gtatggagat aaagaaatat
      961 taaaaaactt aaatttttgt tttcaacgta atgaaaagta tttaatttta ggtgaaagtg
     1021 gaagcgggaa atctacatta ttaaaattat tgaatggctt tttgagagat tatagtggag
     1081 aattgcgatt ctgcggggat gatataaaaa aaacctccta tttaaatatg gtttcgaatg
     1141 ttctatatgt agatcaaaaa gcttatttgt ttgaaggtac gattagagat aatattttat
     1201 tggaagaaaa ttatactgat gaagaaatac tacagtcttt agagcaagtt ggtttgagtg
     1261 taaaagattt tcctaataac attttagatt attatgttgg tgatgatggg agattactgt
     1321 caggagggca gaaacaaaaa attactttag ctagagggct aattagaaat aagaaaatag
     1381 tattaattga cgagggaact tctgctatcg ataggagaac ttcgttagcg attgaacgta
     1441 agatattaga tagagaggat ttgactgtca ttattgttac ccatgctccg catccggaac
     1501 ttaaacaata ttttactaag atatatcaat ttccaaagga ttttatttaa gaatataggg
     1561 gagggaaaca tgagattcta ttgtgaagct tatgaggtaa ttactgaaga gaaaaaagta
     1621 tcattttttt atgtagatga gaaaccctta aggtatctgt tatttgacta tatatttcta
     1681 ctagttctag ctaaaaaaaa tatcccaatc ttatgaactg caccccaaaa gttagattta
     1741 ttctgtctaa cttttggggt gcagttcatt ttgcatagtt ttggcattca tcctagatat
     1801 ccatataaaa tgtctgagat tgttgaaatg tctaaaaaaa tatcagatct aaacgataaa
     1861 aaagaatttc acaaaaagaa ctgtgagttg aggtttcaag atagtgttct tgataacata
     1921 aatagtgtcc cg
//
LOCUS       DAVGJL010000178         1888 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.179, whole genome shotgun sequence.
ACCESSION   DAVGJL010000178 DAVGJL010000000
VERSION     DAVGJL010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.179"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            162..1508
                     /gene="gdhA"
                     /locus_tag="VVE42_001978"
     CDS             162..1508
                     /gene="gdhA"
                     /locus_tag="VVE42_001978"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEU3074115.1"
                     /translation="MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVVSFRVPWVDRDGKIQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHEGTVWTYAGNYDIALPCATQNEINGEAAKR
                     LVAQGVICVSEGANMPSDLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDGRLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGIV"
BASE COUNT          594 a          354 c          379 g          561 t
ORIGIN      
        1 agatatttcc aagaaaaacg ttcgtttttt tatctatttt caaaaaaacc aaaaactgaa
       61 ttgaaagaat ttttagaaaa tttctgtttt ttctttacag tgaaaaaaaa ttctgctata
      121 atgtcttatg taataaaata tgacaataaa aggagaacga tatgacatct gctaaagaat
      181 atatccaaag cgtgtttgaa actgtgaaag ctcgtaacgg gcacgaggct gaattcctcc
      241 aagctgttga agaatttttc aacactttag aacctgtatt tgaaaaacac cctgagtata
      301 tcgaagaaaa tatcttggca cgtattactg agcctgagcg cgtggtttct ttccgtgttc
      361 cttgggttga ccgtgatgga aaaattcaag taaaccgtgg ttaccgtgtt caattcaact
      421 cagctgttgg accatacaaa ggtggacttc gtttccaccc aactgtaaac caaggaatct
      481 tgaaattcct cggatttgaa caaatcttta aaaatgtctt gactggactt cctatcggtg
      541 gaggtaaagg tggatcagac ttcgatccta aaggtaaaac agatgctgaa gtgatgcgct
      601 tctgccaaag cttcatgact gaattgcaaa aacacatcgg accatcactt gacgtacctg
      661 ctggtgatat cggtgttggt ggacgtgaaa ttggttacct ttacggtcaa tacaaacgtc
      721 ttaaccaatt tgatgctggt gtcttgactg gtaaacctct tggatttggt ggtagcttga
      781 ttcgtccaga agcaactggt tacggtttgg tttactatac tgaagaaatg ctcaaagcta
      841 acggtaacag ctttgctggt aagaaagtcg ttatttcagg ttctggtaac gttgctcaat
      901 atgctcttca aaaagcaact gaactcggtg caactgttat ttctgtttct gactcaaatg
      961 gttatgtcat cgatgaaaat ggtatcgact tcgatctttt ggttgatgtt aaagaaaaac
     1021 gtcgtgctcg tttgactgag tatgcagctg aaaaagcaac tgcaacctac cacgaaggta
     1081 ctgtatggac ttacgctgga aactatgaca ttgctcttcc atgtgccact caaaacgaaa
     1141 tcaacggtga agcagctaaa cgtttggttg ctcaaggcgt tatctgtgta tctgaaggtg
     1201 ccaacatgcc gagcgacctt gatgccatca aagtttacaa agaaaatggt atcttctacg
     1261 gacctgccaa agctgccaac gctggtggtg tagccgtttc agcccttgaa atgagccaaa
     1321 acagtcttcg cctctcatgg actcgtgaag aagttgatgg ccgtctcaaa gacatcatga
     1381 caaacatctt caacacagct aaaacgactt cagaaacata cggtcttgat aaagactacc
     1441 ttgcaggagc aaacattgct gcctttgaaa atgtagcaaa cgctatgatt gcccaaggta
     1501 ttgtttaaga tttatttgct taatcctcaa tcactacgat tggggatttt tagttgagtt
     1561 agacagttgg gaaattctaa ttaaatgaaa taaaaactga acaaattgat tgcggaattc
     1621 aaaccaattt ctaacatatt tctagaaatt aatttgactt ttctaatcga tttgttcata
     1681 tcttatttca atttactata cttacttgac cctttttata ctcaatgaaa atcaaagagc
     1741 aaactaggaa gcgagccgca ggctgtactt gagtacggca aggcaaagct gacgtggttt
     1801 gaagagattt tcaaagagta ttaaacaaaa agaaaaaccg ctactcctaa gaatagcgat
     1861 ttaatcatgt ttttaagcta ctactgta
//
LOCUS       DAVGJL010000179         1887 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.180, whole genome shotgun sequence.
ACCESSION   DAVGJL010000179 DAVGJL010000000
VERSION     DAVGJL010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.180"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            32..715
                     /locus_tag="VVE42_001979"
     CDS             32..715
                     /locus_tag="VVE42_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000507009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3074116.1"
                     /translation="MGFSPGIAVFYKESQTTKNLFKFYYFLYFTTLISYYFFFTFVYD
                     KPLLLPLIPFSIIIALVQKLYRIENQQLFLLKSKVLTILESKRDCEFNLQDYHEIWKL
                     QSKSELPCVALSYISLIKPYLSESVREQIDLLEVKRFKKINYPISLYGMLDVIKLNLY
                     LRHYNEKNKYESMLKKILEVRPDFVLIEQNIDDSLNSSQPLSLSLAISEIQLLLEVYI
                     GIKHVSIRR"
     gene            1187..1561
                     /locus_tag="VVE42_001980"
     CDS             1187..1561
                     /locus_tag="VVE42_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0224 family bacteriocin-like peptide"
                     /protein_id="HEU3074117.1"
                     /translation="MELVLPNNYVVLEQEEMMYLDGGFSIPRWPVATAINIAFNGVLG
                     GGAISLVRNYIRNYGLRRVTSAIAGAAARYVGVRVANRVAGFALSAINGFAAWMSIGD
                     AITTIWANNDVNRRDPNLNALW"
BASE COUNT          634 a          243 c          334 g          676 t
ORIGIN      
        1 gaaatactat cgaattctct ttcaccaact aatgggattt agtccaggaa tagcagtgtt
       61 ttataaggag agtcaaacaa ctaagaatct gtttaaattt tactacttct tgtactttac
      121 aacactaatt tcatactatt ttttctttac ttttgtgtat gacaaaccgt tgcttttgcc
      181 tcttattcca ttttctatta tcatagcatt agtacaaaaa ctttatagaa ttgagaatca
      241 acaattattt ctgttaaaat caaaggtact aacaatactg gaatctaaga gggattgtga
      301 gtttaatctt caggattatc atgaaatttg gaaattgcaa tcaaaatctg agttgccatg
      361 cgtggcttta tcttatatca gtttgatcaa gccatatctt tcagagagcg tgcgtgaaca
      421 aattgattta ttagaagtta agcgatttaa aaaaataaat tatccaatat cactttatgg
      481 tatgttggat gttataaaat taaatctata tttacggcat tataatgaaa aaaataaata
      541 tgaatcaatg ttaaagaaaa ttttagaggt aagaccagac tttgttttaa tcgagcagaa
      601 tattgatgat agtttaaata gctcgcaacc attatcttta tctttagcta tttcagaaat
      661 ccaacttttg cttgaagtgt atatagggat caaacatgtt tcaattagga gataaaacta
      721 gaataacatt tgctattata ggggcaagtt gcatattagt cagtatgtat cttatacctc
      781 tcattaacac ggataataaa ttttgggaaa tacttttatt tatattaggt gtattgctaa
      841 ttggaattgc gttactaagt caaaaaaaaa taaattttgt gttagtgaat acgtaattat
      901 tactattgac tgttttatgg tggaaagcat ccatagcttt ttgtaaaata atctgattgg
      961 ctaatatttg cagtaggtgt tgtctatgat aaagaaacct atcttgattg gattttagaa
     1021 ctgttaagtt tcaagcaata attggtttta aaccttaggt aacctataaa aacaaataag
     1081 gtagagaaag gatatcttat ctaggtagct aagtgataaa aacacagtcg tctatcactt
     1141 gttaggaaac tatcctaact atgcttaata aaaaaggaga aaataaatgg aactcgtatt
     1201 accaaataat tatgttgttc ttgagcaaga agagatgatg tatcttgatg ggggattttc
     1261 tattccgaga tggcctgttg caacagccat taatatagct tttaatggtg ttttaggtgg
     1321 aggagcaatc agtctagtta gaaattatat tcgtaattat ggtttgcggc gagttacaag
     1381 cgcaattgct ggagcagctg caagatatgt tggggtacga gttgcaaata gagtggcagg
     1441 atttgcactg tctgctatta atggatttgc agcttggatg tcaattggcg atgctattac
     1501 aacaatctgg gccaacaatg atgtaaatag gagagaccca aatttaaacg ccttgtggta
     1561 actagatatg ggtattaaaa tggaaaaaat ttttgttatt attttttttg tatgtctatt
     1621 tatatcatcc ataacttttt tagcctatga ttttgttagc gaagaaataa aaaagttgat
     1681 tatttggatg aatgtcgttt ttttgatttt aatcatagca atgataattt atccaaaatt
     1741 aagaaaatga taaggacact gtaaaattat gatacgtaaa ctaatcattt ttttattaat
     1801 gctccctctt tttgtcttgt ggctagagtt gcatgtccta gtgaataact tactattgaa
     1861 tctagaatct ccccttgatt ttgtgat
//
LOCUS       DAVGJL010000180         1867 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.181, whole genome shotgun sequence.
ACCESSION   DAVGJL010000180 DAVGJL010000000
VERSION     DAVGJL010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.181"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(<1..1320)
                     /locus_tag="VVE42_001981"
     CDS             complement(<1..1320)
                     /locus_tag="VVE42_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU3074118.1"
                     /translation="MISRIILQNFKRYQNVDFTCNSEVNIFVGENGAGKSTLLYAVGL
                     VLSGSHSQIERSSLSSIINQEAILEFMSTRDINQIPEVFVEIYFDELSTEISSNFNIE
                     GKHNSQKIKAFGLSLKIIPNQDYIAEITSVLNDSKWTVFPFEFYRVEFLTFSGKTYSS
                     YNKPYKFLHSMINTSLIDTQQEIQKRIHEVYLDNISAENRAKVNHQYRINSFNFLKIL
                     RKDELLKENASEFQLHFDESENSFRNSVSAVKNGVDIKNLGQGEKVLLGVENAYSHLK
                     ETVKILLIEEPENHLSFQNLQKLVRMLSSNTGVQVFIGTHSNMIASRLGVDNLLFLDN
                     GQISKLKGLKKDTVRFFKKSTNQNLLNFSLGKKIILVEGNAEYILVEKFFEILHSKKP
                     EEFNVSIISVDGLSFERYLEIATNFTDKKVVVITDNDGDYESKVQSKY"
BASE COUNT          648 a          316 c          229 g          674 t
ORIGIN      
        1 atattttgac tgtactttac tttcgtaatc accatcatta tccgtgataa ctactacttt
       61 tttatcagta aagttagttg caatttctaa atacctctca aaacttaatc catctacaga
      121 aataattgat acattaaatt cttcaggctt tttggagtgg agtatttcga aaaacttctc
      181 tactaaaata tactcagcat tcccctcaac aagaattatc ttcttaccta gagagaagtt
      241 caataaattt tgattagtag atttcttgaa aaatcttaca gtatcttttt tcaaaccttt
      301 aagtttagat atctgaccat tatctaaaaa aagaagattg tctacaccca atcttgaagc
      361 aatcatattt gaatgagtcc caataaaaac ttgaacacct gtatttgaac ttagcattct
      421 aaccaatttc tggaggtttt ggaagctcaa atgattctca ggttcctcaa taagtagtat
      481 tttaaccgtt tccttaagat gactgtatgc attttcaaca cctaataata ccttttctcc
      541 ttgccctaaa ttttttatat ctacaccatt ttttacagct gatactgaat ttctaaatga
      601 attttctgat tcgtcaaaat gaagttggaa ttctgaagca ttctccttca acagttcatc
      661 cttccttaaa atctttaaaa aattaaatga atttatcctg tattgatgat taacttttgc
      721 tctattctca gctgatatat tatccaaata tacctcatga atacgttttt gaatttcttg
      781 ttgtgtatca ataagacttg tatttatcat tgaatgtaga aacttatatg gtttattgta
      841 agaggaatag gttttccctg aaaatgtcaa aaattcaact ctataaaact caaatggaaa
      901 gactgtccat ttactatcat ttaagacaga agtaatttca gctatataat cttgattggg
      961 aattattttt aatgaaagtc caaaagcttt gattttttga gagttatgtt tcccttctat
     1021 attgaagtta gaggaaattt cagtacttaa ttcatcaaaa taaatttcaa cgaagacctc
     1081 agggatctga ttgatatccc ttgtgctcat aaattctaaa atcgcttctt ggttaataat
     1141 actagacaaa ctacttcttt caatttgact gtgactacca ctcagcacta atccaacagc
     1201 atacaatagc gttgactttc ctgcaccatt ctctccaacg aaaatgttaa cttctgagtt
     1261 acaagtaaaa tcaacattct gataacgttt aaagttttgt aatattattc ttgatatcat
     1321 acagactacc taaacctcgt atattagttt gaatagttat ttattaatat tatatcacat
     1381 aaatggtgtt aatttttcaa aattgtaatt acagataaga tgaatattgc tttattcttg
     1441 catcttaaaa agattttgac ataattgttg cataataaag caataggagc tttagcttat
     1501 attttctagt attcgtctta aagaacacca aatagataaa cactattcct aatctgagct
     1561 atctaatcta ttaaaaatca atagaacttc atacaacaag tggtaaagta gtaaatccgt
     1621 tccagatcaa aaaactcatt ctgctcttgc aagaacactt gatgttccaa caacatattt
     1681 tgaatcagta tacaagatta tgatccccta tcttcagctc tcttcaaaaa attaagattc
     1741 tgatgctgaa aaaagaagaa tccttgattt tatgagtcta gtaaatagtg aatacccaat
     1801 tgatgaaagt tttaattatc aaaactatat ttcaaatgca gaaattccag caaaatatga
     1861 aaactat
//
LOCUS       DAVGJL010000181         1854 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.182, whole genome shotgun sequence.
ACCESSION   DAVGJL010000181 DAVGJL010000000
VERSION     DAVGJL010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.182"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            310..759
                     /locus_tag="VVE42_001982"
     CDS             310..759
                     /locus_tag="VVE42_001982"
                     /inference="COORDINATES: protein motif:HMM:NF015038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU3074119.1"
                     /translation="MVNCEQLEAYRQLEEAALVGCWAHVRRKFFEATPKQADKSSLGA
                     KGLVYCDQLFSLERDWEALPADERLQKRQEHLQPLLEDFFAWCRRQSVLSGSKLGRAI
                     EYSLKYEETFKTILKDGHLVLSNNLAERAIKSLVMGRSKRVQWTLLA"
     gene            831..989
                     /locus_tag="VVE42_001983"
     CDS             831..989
                     /locus_tag="VVE42_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HEU3074120.1"
                     /translation="MSLLETAKRHQLNSEKYLSYLLECLPNEETLVNKEVLEAYLPWT
                     KVVQEKCK"
     gene            1082..1854
                     /locus_tag="VVE42_001984"
                     /pseudo
     CDS             1082..1854
                     /locus_tag="VVE42_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT          624 a          325 c          383 g          522 t
ORIGIN      
        1 ataaactcca aaaaacttga ttttggagta gttataaaaa ctcattcaga taatttatta
       61 tttgattttg atacgatccc tgaaaatata atagtagttg accattttga acttacaatc
      121 gaaaaatggt taagtaaaag tcaacagtta ctatagtttg caaattattt acagaaagaa
      181 caagaattcc taggagatta ttctggctat gttcattgtg acatgttgcg gcagtaactt
      241 aggactttag tcctctagtt ctgcctatgc gatagcagtc caaagtttag gagtaaggcg
      301 acgctaagct tggtaaactg cgaacagcta gaagcttatc gtcaactgga agaagctgca
      361 cttgttggat gttgggcgca tgtgagaagg aagttttttg aagcgacccc caagcaagca
      421 gataaatcat ccttaggagc taaaggttta gtttattgtg atcagttatt ttccttggaa
      481 agagactggg aggctttgcc agctgatgaa cgactacaga aacgtcaaga acatctccaa
      541 cccctactgg aagacttctt tgcttggtgc cgtcgtcagt cagttttatc gggttcaaaa
      601 ctaggaaggg caattgaata cagcctcaag tatgaagaaa cctttaagac cattttaaaa
      661 gacggacatc tggtcctttc caataatcta gctgaacgcg ccattaaatc attggttatg
      721 ggacggagta aaagagtcca gtggactctt ttagcctgag ctcagtttaa aaaagcgagg
      781 gtggttgttt tctcaaagtt tttgaaggag ctaaagcaag agctattatt atgagcttat
      841 tggaaacagc taaacgtcat caactaaata gtgagaaata tctatcctat cttctagaat
      901 gtcttccaaa cgaggaaact ctcgtaaaca aagaggtttt agaggcctat ttaccgtgga
      961 ctaaagttgt acaagaaaag tgcaaataag aaatctccag attaggaact atccgtgagt
     1021 tctctagtct ggagattttt caatagactt cctgcgaaac aaaatatggt ataatagttc
     1081 tatgaatgat gaagcaagta aacaactaac cgatgcacga tttaagcgtc ttgttggtgt
     1141 tcagcgtacc acttttgaag agatgttagc tgtattaaaa acagcttatc aacttaaaca
     1201 cgcaaaaggt ggacgaaaac ctaaattaag cctagaagac cttcttatgg ccactcttca
     1261 atatgtgcga gaatatcgca cttatgaaca attgcggctg attttggtat tcacgaaagc
     1321 aacttaatcc gtcggagcca atgggttgaa gtaactcttg ttcaaaggtg gtgttacgat
     1381 ttcaagaact cctctcagtt ctgaggacac ggtaatgatt gatgcgacgg aagtaaaaat
     1441 caatcgccct aaaaaaaaca attagcgaat gattctggta aaaagaaatt ttacgctatg
     1501 aaggctcaag cgattgtcac aagtcaagga agaattattt ctttggatat cactgtgaac
     1561 tattgtcatg atatgaagtt gttcaaaatg agtcgcggaa atatcggaca agctggtaaa
     1621 atcttggctg acagtaatta tcaagggctc atgaagatat atcctcaagc acaaactcca
     1681 cgtaaatcca gcaaactcaa gccgctaata gctgaagata aagcctataa ccatgcacta
     1741 tctaaggata gaagcaaggt tgagaacatc tttgccaaag taaaaacgtt taaaatgttt
     1801 tcaacaacct atcgaaatca tcgtaaatgc ttcggattac gaatgaattt gagt
//
LOCUS       DAVGJL010000182         1844 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.183, whole genome shotgun sequence.
ACCESSION   DAVGJL010000182 DAVGJL010000000
VERSION     DAVGJL010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.183"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            73..1704
                     /locus_tag="VVE42_001985"
     CDS             73..1704
                     /locus_tag="VVE42_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEU3074121.1"
                     /translation="MSINWQEILFHFLGGLGLFLYSIKTMGDGLQQAAGDRLRFYIDK
                     YTSNPFFGVLVGIGMTALIQSSSGVTVITVGLVSAGLLTLRQAIGIVMGANIGTTVTS
                     FLIGFKLGNYALPMLFIGAVCLFFTKNRTVNNIGRILFGIGGIFFALNLMSGAMAPLK
                     DLQVFKDYMIELSKNPVLGVFVGTGLTLLIQASSATIGILQNLYAGNLIDLQGALPVL
                     FGDNIGTTITAIIASLGANIAAKRVAGAHVAFNVIGTVVCVIFLVPFTVLIHWFEATL
                     NLAPEMTIAFAHGTFNITNTIVQFPFIGALAYFVTKIIPGEDEVVKYEPLYLDEHFIK
                     QAPSIALGNAKKELLHLGNYAAKAFDLSYKYIIGLDEKVAEKGHKTEEAINTIDEQLT
                     RYLIALSSEALSQKESEVLTNILDSSRDLERIGDHTEALLNLTDYLQRKNVEFSDAAL
                     KELEEVYRQTSDFIKDALDSVENNDIEKARSLVERHEAINKIERVLRKTHIKRLNKGE
                     CSTQAGVNFIDIISHYTRVSDHAMNLAEKVFAEQI"
BASE COUNT          530 a          388 c          365 g          561 t
ORIGIN      
        1 ttgttagatt tacttttatc ctttaaaata gaataggaga tattccgcga ttatttttcg
       61 gaggaaaaag aaatgtccat taattggcag gaaattttat ttcacttttt aggtggtctg
      121 gggctattct tatatagcat caagaccatg ggagacggtt tacaacaagc tgctggagat
      181 cgccttcgtt tttacattga caaatatact agtaatcctt tctttggagt tctggttggt
      241 attgggatga ctgctctaat tcagtctagt tctggtgtaa cagttattac agtcggcctg
      301 gtcagtgccg gtctcttaac cttacgtcag gctatcggga ttgtcatggg tgctaatatt
      361 gggacaactg tcacatcctt tctcatcggt tttaaattag gtaactatgc cctacctatg
      421 ctctttatcg gtgccgtctg tctctttttt acaaaaaatc ggacagtcaa taatatcgga
      481 cgcatcctct ttggtatcgg tggtatcttt tttgccctca atctcatgag cggcgcaatg
      541 gctccactca aggatttaca ggtctttaag gactatatga ttgagctaag taagaatcct
      601 gttttgggtg tctttgtcgg tactggcttg accttgctaa ttcaagcttc ttcggctacc
      661 attgggattt tacaaaacct ctacgccggc aatctaattg acctacaggg agctttgcca
      721 gttctatttg gtgacaatat cgggacaacc attacagcca tcattgcctc tttaggggct
      781 aatattgcag ctaaacgggt agcaggagct catgttgcct tcaacgttat cggaacagtc
      841 gtctgcgtta tttttctagt tccttttact gtcctgattc attggtttga agctacgcta
      901 aatctagcac cggaaatgac catcgccttt gctcacggaa cctttaatat taccaacacc
      961 attgtccaat ttccatttat cggagctctg gcttactttg taaccaagat tattcctgga
     1021 gaggacgagg ttgtcaaata cgaaccctta tatcttgatg aacatttcat caaacaggcc
     1081 ccatctatcg ctctaggaaa tgctaagaaa gagctcttgc acttaggaaa ctacgctgct
     1141 aaagcctttg acctttccta taagtacatc attggcttgg atgaaaaagt tgctgaaaaa
     1201 gggcataaaa ccgaagaagc aattaacacc atcgatgagc aattaacacg ttatctcatt
     1261 gccctttcaa gcgaagctct cagccaaaaa gaaagtgaag tgcttaccaa tatccttgat
     1321 tcctcccgtg atttggaacg gattggagac cacacggagg ctctactcaa tctgactgac
     1381 tatcttcaac ggaaaaatgt tgaattttct gatgccgcct tgaaagaatt agaggaagtt
     1441 taccgccaaa ctagtgactt tatcaaagat gctctggata gtgtggaaaa caatgatatt
     1501 gaaaaagcac gcagtcttgt agaacgtcat gaagcaatca ataagataga acgtgttctc
     1561 agaaaaaccc acatcaaacg cctcaacaaa ggcgaatgtt caacacaagc tggggtcaac
     1621 tttatcgaca tcatctcaca ctacactcgt gtatcagacc acgctatgaa ccttgctgaa
     1681 aaggtttttg cagaacaaat ctaagaacca agaagctatc catcataatt ggatggcttc
     1741 ttactttttc ctaagcaaga ctaggctgaa tgaaactgaa agagtattct gcagatatat
     1801 agtatattga aactagaata gtacacctct atttctaaaa catt
//
LOCUS       DAVGJL010000183         1820 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.185, whole genome shotgun sequence.
ACCESSION   DAVGJL010000183 DAVGJL010000000
VERSION     DAVGJL010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.185"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(<1..1618)
                     /locus_tag="VVE42_001986"
     CDS             complement(<1..1618)
                     /locus_tag="VVE42_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec-dependent serine-rich glycoprotein
                     adhesin"
                     /protein_id="HEU3074122.1"
                     /translation="MFKVMRGGVDTTQVMTETVEDKVSHSITGLDILKGIAAVGAVIS
                     GTVATQTKVFTNESAVLEKTVEKTDALATNGTVVLGTISTSNSASSTSLSASESESTS
                     ASESASTSASTSASTSASESASTSASISISASSTVVGSQTAAATEATAKKVEEDRKKP
                     ASDYVASVTNVNLQSYAKRRKRSVDSIEQLLASINAAVFSGNTTVNGAPAINASLNIA
                     KSETKVYTGEGVDLVYRVPIYYKLTVINDGSKLTFTYTVTYVNPKTKALSNISNMRPG
                     YSIYNTGTSIQTMLTLGSGLGTPSGVKNSITNKNGGQVLSYNTSTMTTQGSGYTWGNG
                     AQMNGFFAKKGYGLTSSWTVSITGTDTSFTFTPYAAKTDRIGTNYFNGKGKVVESSTT
                     SQSLSQSKSLSVSASQSASASASTSASASASTSASASASTSASESASTSASASASTSA
                     SESASTSASASASTSASASASTSASESASTSASASASTSASASASTSASASASTSASA
                     SASTSASASASTSASASSSTSASESASTSAS"
     gene            complement(1616..1729)
                     /locus_tag="VVE42_001987"
                     /pseudo
     CDS             complement(1616..1729)
                     /locus_tag="VVE42_001987"
                     /inference="COORDINATES: protein motif:HMM:TIGR03715.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="KxYKxGKxW signal peptide domain-containing
                     protein"
BASE COUNT          480 a          385 c          397 g          558 t
ORIGIN      
        1 ctgatgcact ggttgacgct gattcagatg ctgaggtgct tgatgaagct gacgcacttg
       61 tacttgctga agctgatgca ctcgttgatg ctgaagccga ggcactggtt gacgctgaag
      121 ccgacgcact cgttgatgct gaagctgacg cactggtact tgctgaggct gacgcactgg
      181 ttgacgctga ttcagatgct gaggtgcttg ctgaggccga ggcgctggtt gatgcagaag
      241 ctgacgcact ggttgatgcc gattcagaag ctgatgtgct tgctgaggct gacgcactcg
      301 ttgatgctga ctcagaagct gaggtgcttg ctgaagctga tgcactcgtt gatgctgaag
      361 ctgacgcact ggtgcttgct gaagctgagg cgctttgact agcacttact gagagtgact
      421 tagactgtga aagtgactga ctggtcgtgc tagattcaac tacctttcct ttaccattga
      481 agtagttagt tccgattcta tctgttttag cagcgtaagg ggtaaatgta aaggatgtat
      541 ccgttccagt aattgataca gtccaagatg atgttaatcc atatcctttc ttagcaaaga
      601 aaccattcat ttgggcacca tttccccaag tatacccact accctgcgtc gtcattgtag
      661 atgtattata ggatagaacc tgtccaccat ttttgttagt aatggagttc tttacacctg
      721 aaggggtacc aagaccactg ccaagggtta acattgtttg tattgaagtt cctgtattat
      781 agatagaata tccaggacgc atatttgata tattactaag agcctttgtt ttaggattca
      841 catacgtaac cgtataggta aaggtcaatt ttgaaccatc atttatcact gtcaatttat
      901 agtaaattgg aacacgatat accaaatcta caccttcacc tgtataaact tttgtctcac
      961 ttttagcaat gtttagactt gcattaattg caggggcgcc atttacagtc gtattgccag
     1021 aaaaaacagc agcatttata gaagccagca attgctcgat ggaatccact gaacgcttgc
     1081 gtcgcttagc ataagattgg agattgacat ttgtaactga tgctacataa tcactagctg
     1141 gtttcttacg atcttcttcg accttcttag cagttgcttc tgtagcggca gctgtttgtg
     1201 atcctaccac agtagatgat gcagagatac ttatcgaagc cgatgtactt gctgattctg
     1261 atgcacttgt actcgctgag gtcgaagcgc tggttgaggc tgactcagat gcagatgtac
     1321 tttccgactc tgaagctgac aaactagttg aactcgctga attacttgta gatatcgtac
     1381 ctagaactac tgtaccattt gttgccaaag catccgtttt ctctacagtt ttttcaagta
     1441 ctgctgactc atttgtaaat accttcgttt gagttgcaac ggttccactt atgacagctc
     1501 ccacagcagc tattcccttg aggatatcaa gcccagtaat tgaatgactt actttatctt
     1561 ctaccgtttc ggtcatgacc tgagtagtat caacaccacc acgcatgacc ttaaacaagc
     1621 caaaaagaga agtcgaggcc cgtagccaat gcttccctga tttgattaat ttgaaccgag
     1681 ttactcggtc tgtttctcta tattcactct tctgacgtct aaaaaatatt ttgaaatttc
     1741 ctttcgagta ttattttcat gtatggatat aaataaatta ccatactata attatagaat
     1801 agggaaccca aaatagcaag
//
LOCUS       DAVGJL010000184         1816 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.186, whole genome shotgun sequence.
ACCESSION   DAVGJL010000184 DAVGJL010000000
VERSION     DAVGJL010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.186"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(228..1802)
                     /gene="pspC"
                     /locus_tag="VVE42_001988"
     CDS             complement(228..1802)
                     /gene="pspC"
                     /locus_tag="VVE42_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein PspC,
                     choline-binding form"
                     /protein_id="HEU3074123.1"
                     /translation="MFASKSERKVHYSIRKFSIGVASVVVASLFLGGVVHAEEVGGRN
                     TPTVTSSGQDISKKYADEVESHLKKILSEIQTQLDRKRHTETVALINELQGIKKTYLY
                     NLNVLKEKSKVELPSKIKAKLDVAFDQFKKDTLKPGEKVAEAQKKVAEAKKKAEDQKE
                     EDRRNYPTNTYKTLELDIAESDVKVKEAELELVNEEAKPRNEEKIKKAKAKVESEKAE
                     ATRLEKIKTDRKKAEEEAKRKAAEEDKVKEKPAEQPQPAPAPQPEKPAEEPNPAPAPK
                     PEKPAEQPKAEKPADQQAEEDYARRSEEEYNRLTQQQPPKPEQPAPAPKTGWKQENGM
                     WYFYNTDGSMATGWLQNNGSWYYLNSNGAMATGWLQYNGSWYYLNANGDMATGWFQYN
                     GSWYYLNANGDMATGWFQYNGSWYYLNANGDMATGWLQYNGSWYYLNSNGAMVTGWLQ
                     NNGSWYYLNANGSMATDWVKDGDTWYYLEASGAMKASQWFKVSDKWYYVNGSGALAVN
                     TTVDSYRVNANGEWVN"
BASE COUNT          402 a          402 c          332 g          680 t
ORIGIN      
        1 aaatttgtcc tttctcgagc ttagctgact tcaacccact acagttgaca aagagccaaa
       61 aaaaactccg tagtctctag cgctataaat ccggctagag attacagagt tattttttaa
      121 tctaaacttc ctacaagggt atattatcta ctgaaaggat atggtatttg tagggaatac
      181 tttaaagagt tcttacagga agtcagtatt aactagttat attaagttta gtttacccat
      241 tcaccattgg cattgactct atagctatct acagttgtgt tgactgcaag ggcacctgag
      301 ccattgacat agtaccattt atctgatact ttgaaccatt ggcttgcttt catagcacct
      361 gatgcttcaa gatagtacca ggtatctcca tctttcaccc aatctgttgc cattgaaccg
      421 ttagcgttta ggtagtacca tgagccattg ttttggagcc atcctgttac catagcacca
      481 ttgctgttta ggtagtacca tgaaccattg tattggagcc atcctgtcgc catatcacca
      541 ttagcgttga ggtagtacca tgaaccattg tattggaacc atcctgtcgc catatcacca
      601 ttagcgttga ggtagtacca tgaaccattg tattggaacc atcctgtcgc catatcacca
      661 ttagcgttga ggtagtacca tgaaccattg tattggagcc atcctgtcgc catagcgcca
      721 ttgctgttga ggtagtacca tgagccattg ttttggagcc atcctgtcgc cattgaacca
      781 tcagtattgt agaagtacca cataccgttt tcttgtttcc agcctgtttt tggtgcagga
      841 gctggttgct ctggttttgg cggttgctgt tgagtcaagc gattatattc ttcttctgat
      901 ctacgagcat agtcttcttc agcttgttga tcagctggtt tttctgcttt tggttgttca
      961 gctggcttct ctggttttgg agctggagct ggattaggct cttcagctgg tttttctggt
     1021 tgaggagccg gcgctggttg tggttgttca gctggttttt ctttaacttt atcttcttct
     1081 gctgcttttc gtttagcttc ttcttctgct tttttacgat ctgtcttgat tttttctaac
     1141 cttgtagcct cagctttttc actctcaact ttcgcttttg ctttcttaat tttttcctcg
     1201 tttcgaggtt tagcttcctc gtttactagt tcaagctccg cttctttaac tttcacatcg
     1261 gactcagcaa tgtcaagttc aagcgttttg taagtattgg ttgggtagtt acggcgatct
     1321 tcttcttttt gatcctcggc ttttttctta gcttctgcaa ctttcttctg agcttctgct
     1381 accttttctc ctggtttcaa tgtatctttt ttaaactgat caaaagctac gtctaacttt
     1441 gcttttattt ttgacggcaa ctcaactttc gacttctctt ttaaaacatt taaattatac
     1501 aaatacgttt tcttaatgcc ttgcaactcg ttaattagcg ctacagtttc ggtatgtcgt
     1561 tttctatcta attgtgtttg gatctcactc aatatttttt ttagatgcga ctcgacttca
     1621 tcagcatact tcttcgatat atcttgccca ctagatgtaa ccgtgggggt attcctacca
     1681 ccaacctctt ctgcatgaac tactcctcct aagaacaagc tagcaactac tacactagct
     1741 actccaatac taaatttacg aattgaataa tgtacttttc tttcgctttt tgatgcaaac
     1801 atgtttattt ccttct
//
LOCUS       DAVGJL010000185         1782 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.187, whole genome shotgun sequence.
ACCESSION   DAVGJL010000185 DAVGJL010000000
VERSION     DAVGJL010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.187"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..1165
                     /locus_tag="VVE42_001989"
     CDS             <1..1165
                     /locus_tag="VVE42_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3074124.1"
                     /translation="NLIKPTILEKELVSVHGKNHFRADEVFSTQSITKTFIEGILKAD
                     IVIADITTLNQNAIYELGLRHAMKPKSTIIMCDHHTAKISFFDIAHLPQIRYDSDKLN
                     EVDEVNKVKKLLSEYIDSAIKSDETFTDSPAFESALYRVIINDLIDKTEIQSEEALDK
                     SIAELYDQATDLKNSEKYPEAEEIFQQILESGFIDEEILAGYLLSSYKKNESSLANLK
                     MAQEKISKYIDINATTYHKLLGIYGAIFLRIFYITKNKSDLMSAINYYRLGMNFEDRN
                     IYCARNYCANLLKIALVEKDVEVLKEFYYTSVYTAKTILGSLEKIHRKSSEYDDIWFL
                     SNQEDLMVISGLLSKPINDIEGLTERQKKTINEGSIMLSEDLQRIKTVIINGN"
     gene            complement(1162..1734)
                     /locus_tag="VVE42_001990"
     CDS             complement(1162..1734)
                     /locus_tag="VVE42_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3074125.1"
                     /translation="MTNKKIRVFLSRPNPFTENQNLFIKELIKFLRQHNIECVTLQAA
                     EYTPYEVMNSLSEMIQRSYGIIIVAFGQTFISSGTRKMGAEDNPDFFASKETHLKNKW
                     VTSVYCHIEGILALTYNLPMLSIPQENLTEEGILKKGEYSITSPEFSLETKENILMYL
                     QSEGFQKSFHAWKNLLDDKYNFIKGGEINY"
BASE COUNT          634 a          266 c          274 g          608 t
ORIGIN      
        1 taatttaatt aagccaacaa ttttagaaaa agaattagtt tctgttcatg gtaaaaacca
       61 ctttagagct gatgaagttt tcagtacaca gagtataacg aaaacattta tcgaaggtat
      121 attaaaagca gatattgtaa ttgcagatat tactacttta aaccaaaatg ctatctatga
      181 gttaggtcta cgtcatgcta tgaaacccaa gtcaaccatt ataatgtgtg atcatcacac
      241 agctaagatt agcttttttg atattgcaca cttacctcaa atcaggtatg attctgataa
      301 gttaaacgaa gtggatgaag ttaacaaggt aaaaaaactg ctttctgaat atattgattc
      361 cgctataaag tcagatgaga catttactga tagtccagcc tttgaatctg ctttataccg
      421 tgtaataatt aacgatttaa tcgataaaac tgaaattcaa agtgaagaag cgttagacaa
      481 gtcaatagct gaactttatg atcaagccac ggatctcaag aattcagaaa agtatcctga
      541 ggcagaagaa atatttcagc aaattttaga aagtggattt attgatgaag aaattttggc
      601 aggctattta ctatcatcct ataagaaaaa tgaatcttcg ctagcaaatt taaaaatggc
      661 acaggaaaag attagtaaat atatagatat aaacgccaca acatatcata agttattagg
      721 tatttatgga gctattttct tacgtatatt ttatattaca aagaataaga gtgacttaat
      781 gtctgcaatt aattactata ggttaggcat gaattttgaa gatagaaata tttattgtgc
      841 aaggaattac tgtgctaacc tgttaaaaat agccttagtt gagaaagatg ttgaagtttt
      901 aaaggagttc tactatacat ctgtttatac agcaaaaact attttaggat ctttagaaaa
      961 aatacatcgt aaatctagtg aatatgacga tatttggttt ctttccaatc aagaggatct
     1021 aatggtaatt tcaggccttc tttcaaaacc tattaatgat attgagggtt taactgaacg
     1081 acagaaaaaa acaattaatg aaggtagtat tatgttaagt gaggatctcc agaggattaa
     1141 aactgtaatt ataaatggta attaatagtt aatttcccct cctttgataa agttatactt
     1201 atcatcaaga aggtttttcc atgcatgaaa acttttttga aacccttctg attgcaagta
     1261 cattaatata ttttcttttg tttctaatga gaattcaggt gatgttatag aatattctcc
     1321 cttctttaaa atcccttctt cagttaaatt ttcttgagga atagataaca ttggaagatt
     1381 ataagttagc gctaaaatcc cttcaatatg acaataaaca cttgtaaccc acttattctt
     1441 taagtgggtc tctttggatg caaagaagtc tggattatcc tcagctccca tttttctggt
     1501 tccactagaa ataaatgtct gaccaaatgc aacaattata attccataac tcctttgtat
     1561 catctcactc aacgaattca tgacttcgta aggcgtatac tcagcagctt gtaatgttac
     1621 acattcaata ttgtgttgtc ttaaaaattt aatcaattct ttaataaata aattttgatt
     1681 ctctgtaaat ggattaggtc tgcttaaaaa tactcttatt tttttatttg tcatattcgc
     1741 tatctcctac cgataacaat ttattatcac tatacaaagc ac
//
LOCUS       DAVGJL010000186         1773 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.188, whole genome shotgun sequence.
ACCESSION   DAVGJL010000186 DAVGJL010000000
VERSION     DAVGJL010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.188"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..637
                     /locus_tag="VVE42_001991"
     CDS             <1..637
                     /locus_tag="VVE42_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HEU3074126.1"
                     /translation="FKENGEFNHFPGNTVVTNLYTKQDLMEVVDIIQSRYRELPFIDK
                     FTLTPRNSIHMTVIELLCHENRETEFWSSNLPLDTPLQEIHDYFAKQLEIFPLLDEEI
                     HMRITEMGKQNILVEPADEASAKRLEEIRTYVSEKAGVCFPNHDRYQFHISIGYLRIP
                     LTEEEEEEFTKVRAELTEILLEKIPTITVNRIDYTVFEDMRQFVPYHEKFK"
     gene            662..1729
                     /locus_tag="VVE42_001992"
     CDS             662..1729
                     /locus_tag="VVE42_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU3074127.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIAGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDVKEMPKDWT
                     ELGSKYKGKYSIYGLQGGTGRAILASILVRYLDEKGDLGVSEKGWEVAKEYFANAYTL
                     QKGESSVVKALDKESPIQYGMIWGSGALIGQKEQNVEFKVMTPEIGVPFVTEQTMVLS
                     TSKKQALAKEFIDWFGQSEIQVEYSKKFGSIPANKDALKELPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVK"
BASE COUNT          641 a          241 c          347 g          544 t
ORIGIN      
        1 gttcaaagag aatggggaat ttaatcattt ccctgggaat acagttgtaa caaatcttta
       61 tactaaacaa gatttgatgg aagttgttga tattattcaa tcacgttata gagaattacc
      121 atttatcgat aagtttactt taactccaag gaattctatt cacatgactg taattgaatt
      181 gttgtgccat gaaaatcgtg aaacggagtt ttggagcagt aatcttcctc tagatacacc
      241 tttacaggaa atacatgatt actttgctaa acaacttgaa atttttccat tgttggatga
      301 agaaattcat atgcgtataa ctgaaatggg aaaacaaaac atactagttg aacctgcaga
      361 tgaagcttct gcaaagagat tagaagaaat tcgtacttat gtctcagaaa aagcaggtgt
      421 ttgtttccct aatcatgata gatatcaatt ccatatttca attgggtatc ttcggattcc
      481 tctaaccgaa gaggaggaag aagagtttac taaagtcaga gcagaattaa ctgaaatttt
      541 attagagaag attccaacta ttactgtaaa ccgtattgac tatactgtat ttgaagatat
      601 gagacaattt gttccatatc acgaaaaatt taaataaaat aataaaaaag gagaacttat
      661 catgaaaaaa atgaaagttt ggtctactgt acttgcaacg ggagttgctc ttactacact
      721 tgctgcttgc tctggaggtt caaattctac gactgcttct tcatctgaag aaaaagctga
      781 taaaagtcaa gaattagtaa tctattcgaa ctcagtctca aatggtcgtg gtgattggtt
      841 aactgctaaa gcaaaagaag ctggttttaa tataaaaatg gttgatattg ctggcgctca
      901 attagcagac cgtgttattg ctgagaagaa taatgcagtt gcagatatgg tatttggaat
      961 tggtgctgtt gactcaaata aaattagaga tcaaaaatta ctagtacagt acaagcctaa
     1021 atggttagat aaaattgatc aatctttatc agataaagat aattattata atcctgtgat
     1081 tgttcaacca ttagttttaa ttggggcgcc tgatgtaaaa gaaatgccta aagattggac
     1141 tgaattaggt agtaagtata aaggtaaata ttcaatttat ggtcttcaag gtggtacagg
     1201 acgggcaatt ctagcaagta tcttagttcg ttaccttgat gaaaaaggag atttaggcgt
     1261 atcagaaaaa ggatgggaag ttgcaaaaga atactttgca aatgcctata ctcttcaaaa
     1321 aggtgaaagc tcagttgtta aagcgttaga taaagaaagc ccaattcaat acggtatgat
     1381 ctggggttct ggagcgttaa ttggacaaaa agaacaaaat gttgaattca aagttatgac
     1441 tcctgagatt ggtgtaccat ttgtaactga acaaactatg gttttaagca ctagtaaaaa
     1501 acaagcgtta gctaaagaat ttattgattg gtttggtcaa tcagaaattc aagtagaata
     1561 tagtaaaaaa tttggatcta ttcctgccaa taaagatgcc ctaaaagagt tacctgaaga
     1621 tacgaaaaaa tttgttgatc aagtgaaacc acaaaatatt gactgggaag ctgttggaaa
     1681 gcatttggat gaatgggtag aaaaagctga attagaatac gtaaaataag aaataagagt
     1741 tattttttat gtccaagtct ctctattatt tct
//
LOCUS       DAVGJL010000187         1756 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.189, whole genome shotgun sequence.
ACCESSION   DAVGJL010000187 DAVGJL010000000
VERSION     DAVGJL010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.189"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(<1..661)
                     /locus_tag="VVE42_001993"
     CDS             complement(<1..661)
                     /locus_tag="VVE42_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU3074128.1"
                     /translation="MVLSTSAILVACGKTDKEADAPTTFSYVYAVDPASLGYSIATRT
                     SRTDVIGNVIDGLMENDKYGNVAPSQKDYDLNSTGWAPSYQDPASYLNIMDPKSGSAM
                     KHLGITKGKDKDVVAKPGLDKYKKLLEDAVSEITDLEKRYEKYAKAQAWSTDSSLLMP
                     TASSGGFPVVSNVVPFSKPYSQVGIKGEPYIFKGMKLQKDIVTTKEYNEVFKKWQKEK
                     LE"
     gene            complement(717..1739)
                     /locus_tag="VVE42_001994"
     CDS             complement(717..1739)
                     /locus_tag="VVE42_001994"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEU3074129.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGTGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
BASE COUNT          507 a          389 c          304 g          556 t
ORIGIN      
        1 attccaattt ttctttttgc cattttttaa aaacctcgtt atattctttt gttgtaacaa
       61 tatctttttg caatttcatt cctttaaaga tatatggttc ccccttaata ccaacttgtg
      121 agtatggttt tgagaatggt actacgttac ttacaactgg aaaaccacca gatgaagctg
      181 ttggcatcaa taatgaacta tctgtcgacc aagcttgagc tttggcatat ttttcatatc
      241 tcttctctag gtcagtgatc tcagaaacag catcttctaa caatttctta tatttatcca
      301 aaccaggttt agctacaaca tccttatctt ttcctttcgt aatgccaagg tgtttcatgg
      361 cagaaccaga ttttggatcc ataatattca agtaagacgc tggatcttga tagcttggag
      421 cccatcctgt actgttcaaa tcatagtctt tttgagaagg agcaacattg ccgtatttat
      481 cattttccat caaaccatca ataacatttc caataacgtc tgtcctcgat gttcgagtcg
      541 ctatactgta gcccaatgat gctggatcta ctgcatagac ataagaaaat gttgtcggtg
      601 catctgcttc tttatcagtt tttccacaag ccactaaaat agctgacgtg ctcaggacca
      661 ctcctgctgt taagagccac tttttctatt tcataaaaaa tctcctttgg tttattttaa
      721 tctactttta caatccaacc ttctggcgct tcaatatcgc caaactgaat acccgtcaat
      781 tcattatata atttacgcgt cacaggacct acttctgttt cactatagaa tacatggaaa
      841 tcatcaccat gttgaatacc tccaattgga gaaataaccg ctgctgtacc acaggcacct
      901 gcctctacaa aacggtcaag attatcaatt ggaacatcac cctcaatagg agttaatccc
      961 aagcgatgtt ctgccaaata aagcaaggaa tacttggtaa tagatggcaa gatagatgga
     1021 ctcaatggtg ttacaaattc attatcagct gtaattccaa agaagttagc tgatccgact
     1081 tcttcaatct ttgtatgagt tgatgggtcc agatagataa catctgagaa atgacgtgac
     1141 ttggccattt ttcctggtaa gagacttgca gcatagtttc caccaacctt agccgcacct
     1201 gtaccatttg gtgctgcacg gtcgtactca tcctgaatca agaagttggt tgggaccaaa
     1261 ccacctttaa agtaatttcc aactggcata gcaaagatgg tgaaaatgta ctcttctgcc
     1321 ggttttaccc cgataatatc tccgacacca atcaaaagag ggcgaagata taaggttcca
     1381 cctgttccgt atggtggtac gtattcttca ttcgcacgga caactgcttt acaagcttct
     1441 acaaacatgt ctgtcggaac ttgtggcatc aagagacggt cacatgtacg ttgcagacgt
     1501 ttagcatttt catcaggacg gaacagttga acactgccat ccttagtacg ataagctttc
     1561 aaaccttcaa atgcttgttg tccatagtga agacttggag aagactctga aatatgcaaa
     1621 gttgcatcct ctgtaagctc tccttgatcc cattgtccat ttttgaaatg agcaagatag
     1681 cgataaggta atttcatata ggaaaaaccg aggttttccc aatcaatcgt tactgtcata
     1741 ttccactcct tattct
//
LOCUS       DAVGJL010000188         1670 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.190, whole genome shotgun sequence.
ACCESSION   DAVGJL010000188 DAVGJL010000000
VERSION     DAVGJL010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.190"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            318..503
                     /locus_tag="VVE42_001995"
                     /pseudo
     CDS             318..503
                     /locus_tag="VVE42_001995"
                     /inference="COORDINATES: protein motif:HMM:NF024172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1100..1384
                     /locus_tag="VVE42_001996"
     CDS             1100..1384
                     /locus_tag="VVE42_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3074130.1"
                     /translation="MVWYDYMIQASKQSQFNASHWFRYLRKVIFEDYSYLTNQDVEKL
                     LDSKELTRFQKISLKYAFQEHTPTHKYVISLNKPAKLTNVQKLMEKYKHG"
     gene            1377..1526
                     /locus_tag="VVE42_001997"
     CDS             1377..1526
                     /locus_tag="VVE42_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3074131.1"
                     /translation="MDKMKPVFQALNKELIQENLTLTIICVDGYVLEYHGLRATQDVD
                     AFMAL"
BASE COUNT          640 a          291 c          243 g          496 t
ORIGIN      
        1 gcgccacata gtactgaact ctctccaaaa acggttatgc ctctttgaat agggcgttat
       61 cacaagaaaa gcatctccac gtttcaactt catatggctc aaaaacaatc aattgatgct
      121 aaaacctgta cctagatgtt tcggttcata aaaccatgaa actgtaaaag tggatgaaat
      181 tgatagcgat agtcaaatca agaggcatca taactctaaa aagtcacaat atataagttc
      241 atcctcggaa aaatatcatt ctaattgttg aaatgcctac atgaaaagaa acgtcaaata
      301 ctcatgaaac aacgaataca ggtatcaaaa ctatgacaaa acaagtccct aaatttacta
      361 aagacactgc tcaactttac acctgtaaat ggttgttgta taataaagtt acaaagatgt
      421 acgaccacac tgttgtaaat catagtgttc gcgaatatat tactgatagc atttctacaa
      481 atacaattga aaaggctgga taggctttaa acgaaatatg tcagtgtttt ggcattccta
      541 gccttcatat catttaaaga attgtataga caaaattttt cccaatacag acactcgtaa
      601 caactgcttc atttttctac caacatattt aggaacagga taagatacaa gaggattaat
      661 ccatagatca gttctatacc aatctaagac aaataagcta aaaaaacgat tgataataag
      721 caaatagatt ccaaattttc tctatctgct cattttaata aacaatacta gtgtaactat
      781 ccttccagtc agaagcttgt caaatcacac cgaaaattct tctaaaattt atctcgttag
      841 gcaatcaagc aaaaactcga cgatagtaca aacattatca tacagaattg acttcctaaa
      901 ttatatactt tagtaaggtt ttcggataag aaaaaaggtt cattttacat ttctaaacat
      961 tcttttctaa gatgaaaaac agaatttttc gattgtgatt taaagcaaca agaagatttt
     1021 cagtatcatc ctatagatac gagctaatta agaaaaacta catttttgaa tataaactaa
     1081 attttatagg aggaaaacaa tggtttggta cgattatatg atacaggcat ccaaacaatc
     1141 acaattcaac gcaagccatt ggtttcgcta tttgcgaaaa gttatttttg aagactattc
     1201 ttatttaaca aaccaagatg tagaaaagtt gctagactcc aaagaactaa cccgttttca
     1261 aaaaattagc ttgaagtatg cctttcaaga gcatactcca actcataaat atgtgatttc
     1321 attaaataaa cctgctaagt taaccaatgt tcaaaaattg atggagaaat acaaacatgg
     1381 ataaaatgaa accggtcttc caagccctaa ataaggaatt aattcaggaa aatctgactt
     1441 taacaattat ctgtgtcgat ggttatgtct tagaatatca tggtttacgt gccacacaag
     1501 atgttgatgc ttttatggct ctataatatt tgtagtgggt aaccccccct atggatatta
     1561 tggagcctat tttgtgtaga aaaaaagtcc catatgacct ataatgaaaa gcgacaaaac
     1621 aactcattag aaagaatcat atggaacaat tacattttat cacaaaatta
//
LOCUS       DAVGJL010000189         1658 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.191, whole genome shotgun sequence.
ACCESSION   DAVGJL010000189 DAVGJL010000000
VERSION     DAVGJL010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.191"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            111..518
                     /locus_tag="VVE42_001998"
     CDS             111..518
                     /locus_tag="VVE42_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU3074132.1"
                     /translation="MARYGVYAVIPNPEQKQIVLVQEPNGAWFLPCGEIKAGENHQEA
                     LKRELIEELGFTAEIGTYYGQADEYFYSRHRDTYYYNPAYLYEATSFKEVQKPLENFN
                     HIAWFPIDEAIQNLKRGSHKWAIESWKKQHKIG"
     gene            683..982
                     /locus_tag="VVE42_001999"
     CDS             683..982
                     /locus_tag="VVE42_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEU3074133.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKTSVDEANIKTLYSEKLIGISIPIK"
     gene            1243..1566
                     /gene="tnpB"
                     /locus_tag="VVE42_002000"
                     /pseudo
     CDS             1243..1566
                     /gene="tnpB"
                     /locus_tag="VVE42_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          561 a          318 c          296 g          483 t
ORIGIN      
        1 cattgtgtac tatttttggt tcattttact ataataataa cgatactaga taatgaggaa
       61 aatgcaatgg caaacccaac tttcggagaa aaaaagcgaa tacagactat attgctcgct
      121 atggcgtata tgcagttatc cctaatcctg aacaaaaaca aattgttctt gttcaagaac
      181 caaatggtgc ttggttccta ccatgtggag aaattaaagc aggtgaaaat catcaggaag
      241 ccctaaagcg tgagttgatt gaagagcttg gtttcacagc agaaattggt acctattacg
      301 gacaagctga cgaatatttc tattctcgtc atcgtgatac ctactactac aatcctgcct
      361 acctctatga agcaacttct ttcaaagaag tacaaaagcc actagaaaac tttaatcata
      421 ttgcctggtt ccctattgac gaggctatcc aaaaccttaa acgtggtagc cataaatggg
      481 ccattgaatc ttggaaaaaa cagcataaga ttggctaata ctcttcgaaa ataaaattca
      541 aaccacgtca gcgtcgcctt accgtactca agtatagcct gcggctagct tcctagtttg
      601 ctctttgatt ttcattgagt ataaatcaag cgactttatt caaaaagtgc tataatagaa
      661 ttagaaaatc ggaggaaata ttatgaagct tatcaatacg acaaactcac actcgcaact
      721 ggtaaaaagt cagctagaaa gtaccgatgc aacccttgtt gaggtttatt ctgcagggaa
      781 tacagatgtt atttttaccc aagctccgct tcactatgaa atcctcatct caaacaaaca
      841 ccgtgctatt cgtgagcctg aaatcgaaac cattcaagaa tttttcttga aacgtaaaat
      901 tgataagacc tctgttgatg aagccaatat caagacactc tactcagaga aattaattgg
      961 aatttcaatc ccaatcaagt aagaaaattg agtaaaaact cgatttgaga gaaagtccaa
     1021 taagtctttc cataacaaaa cgcatagtaa caagagtttc tacacccact attatgcgtt
     1081 tttatcattt tgaaatctac tgctaatcaa tgaagaagca aagatcaaac taggcagcta
     1141 gttacaagtt acgtaagcgc gtcataacaa ggtatctatc ctaattcccc aacttccagt
     1201 atactagata aaaaataatt atcggaggaa aggtattgta ctatgataat tcaactaagt
     1261 gatttaggtc aagttcacct tgtttgtggt aagacagata tgaggtaggg aatagactca
     1321 ttagcctatg tagttaaaac ccactttgaa ttggattctt tctccggtca agtctttctc
     1381 ttttgtggtg gacgtaaaga ccgctttaaa accctttact gggatggtca aggattttgg
     1441 ctactatata aacgctttga gaacggaaaa ctgacttggc taagtacaga aaaggatgtc
     1501 aaagctctcg cacctgaaca agtagattgg ctgatgaagg gcttttctat cactccaaaa
     1561 atatagtaaa ttgaaactag aatagtacac ctctgcttct aaaatattgt tagaaatcga
     1621 tttgactgtc ctgatcgatt tgtcctgttc ttgtttcg
//
LOCUS       DAVGJL010000190         1638 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.192, whole genome shotgun sequence.
ACCESSION   DAVGJL010000190 DAVGJL010000000
VERSION     DAVGJL010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.192"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(76..1045)
                     /locus_tag="VVE42_002001"
                     /pseudo
     CDS             complement(76..1045)
                     /locus_tag="VVE42_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744512.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1206..1577)
                     /locus_tag="VVE42_002002"
     CDS             complement(1206..1577)
                     /locus_tag="VVE42_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3074134.1"
                     /translation="MIKKNHILCEIVPGSVAFACFYLSISISSVFQFLLVVINLASIL
                     VTVYLTREMRIKEFEDDDLVSPRTNQIMFIGLTGFMSIICLYRGITAGESYQQIIAYI
                     GAILCLIIILFLIWGLKYYKK"
BASE COUNT          529 a          333 c          231 g          545 t
ORIGIN      
        1 tcttgtctat aggcaaattc tagccatatg ttattacaaa caaatagtac catttcacct
       61 gtttaccatc tatttttatt tatgaattct ttgtctccac ttaatcatac ctgaagtaat
      121 taagagtgca accgctagac gtaatagatt gtaactttct tccactcctg tattaggtag
      181 taattgttta ctcaaatgac tggttttgga gggagaatca ttcccctcaa ccttggataa
      241 aggagtttgt aacttctcag tattttcaga aatcgtctga gatgactccc ctcgcttctt
      301 cttatctatc attaccaaat cttcagactg aacagttata gcgttagaag gaggaatatt
      361 atccttcaat ggctctctgt tatcactgtt attagtcgaa ttttcctttg aattcttcac
      421 ctctcctgtt ttatcatcac ggacactttc agttactgtt tcctcagaag atttcactgt
      481 ctccgtttgc ttagatgagt ttctcgaata catataatca tacacctgtt gtgcagttgc
      541 tccgtcacca acccatccag atgtcggatg tccatttctt aaatacaaaa ttgttggtgt
      601 tcctggaata ccgacttttt aaaaatagaa actctctcgc ttccccatca aaatccttac
      661 catccaaatc ataatactct aacttctttt caatcaagtt attaaattct ttcaattcag
      721 gagaaaattg acgacaatga gagcaagttt ttctaccaaa ataaataata tgttccaatt
      781 gatcccctgt aaaagattga cgaacagctt caatatctat ctttttaaag tccgtaacat
      841 tcttctcata ctcttctggg gttacaacag aaggcttatg ctccaccttt tccttagccg
      901 atggcaaatc tggcgccaca agagaactat cattcgcctg ggtagatgaa gtaataggct
      961 gttcttctgc atatgcaaga cctgcagtaa tagatgcaaa tacaagtggt acaagtaata
     1021 gcgattttat cttttgtttc ttcataagaa tactccttta catttgttat ctttatctta
     1081 ccttatttaa gattacaaat tcatctaaat cttaaatggt catcgaaaca tcttgaattg
     1141 caaaaacttc attataaaag ggtaagtatt tcaaaaacac ttaccccttt atactttatt
     1201 aagctctact ttttataata cttcaagccc caaatgagaa aaagtataat aatcaagcag
     1261 agaatagcac caatgtaggc aattatttgt tggtaggatt ctcctgctgt gatccctcta
     1321 tacaaacaaa taatagacat aaaacctgtc aagccgatga acatgatttg attagttcta
     1381 ggactaacca aatcatcgtc ttcaaactct tttatccgca tttccctagt gaggtaaaca
     1441 gtgaccaaaa tagaagccaa gttaataacc actaaaagaa attggaaaac tgacgaaata
     1501 gaaatagata agtagaaaca agcaaaggca actgacccag gaacaatctc acaaagaata
     1561 tgattctttt taatcaagaa aatctttcat ttcttttctt tctttttaat taaagaaaaa
     1621 tagatcataa ccgcaatc
//
LOCUS       DAVGJL010000191         1607 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.194, whole genome shotgun sequence.
ACCESSION   DAVGJL010000191 DAVGJL010000000
VERSION     DAVGJL010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.194"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..235
                     /locus_tag="VVE42_002003"
     CDS             <1..235
                     /locus_tag="VVE42_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEU3074135.1"
                     /translation="IFSTWICSSLMQMLLAKEKYMEWSLMTMGKDYVDVLEKLITYPY
                     MALVALGAFLGGILGAYIGKALLKKHFEKAGIV"
     gene            235..930
                     /locus_tag="VVE42_002004"
     CDS             235..930
                     /locus_tag="VVE42_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEU3074136.1"
                     /translation="MPTFSSNAPFNPNPISKILVVFITGLTVMHSINIRFELAIVCLI
                     GILLYLNGYKKTLFKWIILCGILYSLPNFMVLSELNPIVKMFLSIPIVIRMFILPFMA
                     ASFMIKTSDVGAIISSMDKLKISKNVSIPIAVMFRFFPSFKEEKKNIKIAMRVRGINF
                     KNPVKYLEYVSVPLLIISSNISDDIAKAAETKAIENPIAKTRYIRVKIQLIDFVYVLA
                     VAGLIVGGLIWLK"
     gene            918..>1607
                     /locus_tag="VVE42_002005"
     CDS             918..>1607
                     /locus_tag="VVE42_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3074137.1"
                     /translation="MVEIKNLSLDYGEEHILDDISLSIAEGECVLFTGKSGSGKSSLI
                     NSINGLAVRYDNAKTKGEIIIDGKNIKNLELYQISMLVSTVFQNPKTYFFNVNTTLEL
                     LFYLENIGLAREEMDRRLKDILEIFPIKNLLNRNIFNLSGGEKQILCIAASYIAGTKI
                     IVMDEPSSNLDIKSISVLAKMLKILKEKGISIIVAEHRIYYLMDIVDRVFLIDKGKLK
                     KTYTRSEFLKLD"
BASE COUNT          557 a          213 c          301 g          536 t
ORIGIN      
        1 aatcttcagc acatggatat gtagctcttt aatgcaaatg cttttagcaa aagaaaaata
       61 tatggagtgg tctttgatga cgatgggaaa agattatgtt gatgtattag aaaagttaat
      121 aacttatcct tacatggctt tagtagcctt aggtgctttc ttaggaggaa ttcttggagc
      181 atatataggc aaggctctat tgaaaaaaca ctttgaaaaa gcaggcattg tataatgcct
      241 actttttctt caaatgctcc ctttaatccc aatccgatct ctaagatatt agtcgtattt
      301 atcacaggct taactgttat gcatagcata aacatccgtt ttgagctggc aattgtttgt
      361 cttattggta ttttgcttta tttgaatgga tacaaaaaaa cacttttcaa atggattata
      421 ttgtgtggga tactttactc cttgcctaat tttatggtgt tatctgaatt aaaccctata
      481 gttaagatgt ttttgagtat acctattgtt attagaatgt ttattttacc atttatggca
      541 gcaagcttta tgataaagac ctcggatgta ggcgcaataa tttcatcgat ggataagctt
      601 aagatttcaa agaatgtatc catacctatt gcggttatgt ttagattctt cccatctttt
      661 aaggaggaga agaaaaacat caaaatagct atgagagtaa gagggataaa ttttaaaaac
      721 ccagtcaaat atcttgaata tgtttctgtg ccactactca ttatatcatc taatatatca
      781 gatgacattg caaaagcggc agaaacaaag gcaatagaaa atccaattgc caagaccaga
      841 tacattcgcg taaagataca gctaattgat tttgtttatg ttttagcggt tgctggactt
      901 attgtgggag gcttaatatg gttgaaataa aaaatttaag tcttgattat ggtgaagagc
      961 atatattaga tgatatatca ctatccatag ccgagggaga gtgcgtgcta tttacaggaa
     1021 aaagtggaag tggtaagtca tctttaataa attcaatcaa tggactagct gtaaggtatg
     1081 ataacgcaaa gacaaagggc gaaataatta ttgatggtaa gaatataaaa aatttggaac
     1141 tttatcaaat ctcaatgctt gtttcaactg tttttcaaaa tcctaagaca tattttttta
     1201 atgtcaatac gacattagaa ttattatttt atttggaaaa tatcggtctt gcaagagaag
     1261 agatggacag gcgtttgaag gatatacttg agatattccc gataaaaaat cttttgaaca
     1321 gaaatatatt taatctatcc ggcggtgaaa aacaaattct ttgcattgca gcttcttata
     1381 tagcaggtac aaagattata gttatggatg agccttcatc gaatttagat attaaaagca
     1441 taagtgtttt ggcaaagatg ctaaagatat taaaagagaa aggcataagc ataattgttg
     1501 cagagcatag aatttattat ttgatggaca tagttgaccg tgtattttta atagataaag
     1561 gaaagcttaa aaaaacttat actagaagtg aatttttaaa gctagat
//
LOCUS       DAVGJL010000192         1600 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.195, whole genome shotgun sequence.
ACCESSION   DAVGJL010000192 DAVGJL010000000
VERSION     DAVGJL010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.195"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..1247
                     /locus_tag="VVE42_002006"
     CDS             <1..1247
                     /locus_tag="VVE42_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU3074138.1"
                     /translation="IPKIRLSDLLIEVDSWTNFSQEFSHDSTGKPPSEQERKIIFAAL
                     LGLGMNIGLEKMAQSTPGISYSQLANAKQWRFYKEALTRAQSVLVNYQLKLPVADFWG
                     EGKTTASDGMRVPVGVSALKSDVNPHYKSMEKGATMIRSINDRHTTHHIEVASTNTRE
                     ATHTLDGLLYHETDLDIEEHFTDTNGYSDQVFGMTALLGFDFEPRIRNIKKSQLFSIK
                     SPSYYPNLSEDISGKINVKIIEENYDEIKRIAYSIQTGKVSSSLLLGKLGSYARKNRV
                     ALALRELGRIEKSIFMIDYITDSELRRRITHGLNKTEAINALARELFFGRRGKFMERD
                     IRRQLQSASALNVLINAISIWNAVYLQAAYNYLVKIDPEVTKYMKHVSPINWEHITFL
                     GEYKFDLLSIPKHLRELNIKNK"
     gene            complement(1321..1542)
                     /locus_tag="VVE42_002007"
                     /pseudo
     CDS             complement(1321..1542)
                     /locus_tag="VVE42_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT          537 a          286 c          299 g          478 t
ORIGIN      
        1 taattcctaa gataaggctt agtgatcttt taattgaggt ggacagttgg accaactttt
       61 cacaagaatt tagtcatgat tctacaggga aaccgccgag tgaacaagaa agaaaaatta
      121 tttttgctgc tttgctgggt ttagggatga atattggtct tgaaaaaatg gcccaatcaa
      181 ctcctggaat ttcttattct cagttagcca atgccaaaca atggcgcttt tataaagaag
      241 ctctgactcg tgctcaatct gttttggtta attatcagtt aaagcttcct gttgcagact
      301 tttggggtga aggaaaaacc actgcttcag acggaatgcg cgtcccagtg ggcgtctcag
      361 ctctaaaatc cgatgttaat ccacattaca aaagtatgga aaaaggagct acaatgattc
      421 gatcaataaa tgataggcat acgactcatc atatcgaggt tgcttcaact aatacaaggg
      481 aagctactca tacccttgat ggcctacttt atcatgaaac agatctagat attgaggaac
      541 attttactga tacaaatggg tattctgatc aggtgtttgg aatgaccgca ttactaggct
      601 ttgattttga acctcgcatc agaaatataa aaaaatcaca attattttct atcaaatcac
      661 cttcctacta ccctaactta tcagaagata taagcggaaa aatcaatgta aaaattattg
      721 aagaaaacta tgatgaaatt aaacgaatcg cctattcgat tcaaacagga aaagtatcta
      781 gttctttact attaggaaag ctaggctcat acgcacgtaa gaatagagta gctcttgcac
      841 tgagagaact aggtcgcatt gaaaagagca tttttatgat agattatatt acagatagtg
      901 agctacggcg aaggatcact catggactaa ataagacaga agcgattaat gctttagcta
      961 gagaactatt ttttgggcga cgcggaaaat ttatggagcg cgatattcgc cgacaacttc
     1021 aaagtgctag tgcgcttaat gtgttaataa atgcaataag tatatggaac gccgtctact
     1081 tacaagcagc ttataattat ctcgtcaaaa tagatcccga agtaactaag tatatgaagc
     1141 atgtatctcc tattaattgg gagcatatca cttttcttgg agagtataaa tttgacttgt
     1201 tatctattcc taaacactta agagaattga atataaaaaa taaataggcc ttgaaacatt
     1261 ggtttagtgg gaatttgtac cccttatcga tacaaattcc cactaagcgc tcgggacccc
     1321 aggtaagaaa aattcatcaa cggaaacatg aagtaacgat acaaggtcat aaagaacttg
     1381 tatgctgggg tgttgccctt tattttcaat attagttaag taccgtgggt caatttcaat
     1441 caatgctccc acttgttcac gagttaaacc tcgtttcaat cgagcttctt taatggctaa
     1501 accaaaggct ctaaaatcat atttatcttc ttttttacgc atagtagacc acctctatac
     1561 attttattgt tcctactgaa ttaaaaacag gtatagaaaa
//
LOCUS       DAVGJL010000193         1565 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.196, whole genome shotgun sequence.
ACCESSION   DAVGJL010000193 DAVGJL010000000
VERSION     DAVGJL010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.196"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            293..1165
                     /locus_tag="VVE42_002008"
     CDS             293..1165
                     /locus_tag="VVE42_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HEU3074139.1"
                     /translation="MKKSRKLATLGICSALFLGLTACQQQHATSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFDSQDKEEFDILFSRVPTEATTDVKVHAEKMETFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPADDGFLFVEKLTFEEPYQAIKFASKEDCYKYLGTKYADYTGEGL
                     AKPFIMDNDKWVKL"
     gene            complement(1311..1565)
                     /locus_tag="VVE42_002009"
                     /pseudo
     CDS             complement(1311..1565)
                     /locus_tag="VVE42_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
BASE COUNT          514 a          273 c          306 g          472 t
ORIGIN      
        1 tatttttcac ctctaacaat cgcaatctct tctttatcca tgaataaaaa cgatttctat
       61 ttttgtaagt aaagcatcac acgaaatcca cgaaaatgaa aacctttgtt gtgttttcgt
      121 aaaaaatttg ttgacagagc acgaaacgcc tactataatt aaatcaagtt taaggaaata
      181 acaaaaaaac aaatcggagg gaaagacgat gaacacatta aatgagaaag taatcaatat
      241 ctgtaaagca gtagttaaag aaactttaat ccaagacatt taggagaaag aaatgaagaa
      301 atcaagaaaa ttagccacat taggaatctg ttcagctttg tttttaggct tgactgcttg
      361 tcaacaacaa catgctactt ctgaggggac gaatcaaagg caaagcagtt cagcgaaagt
      421 tccatggaaa gcttcataca ccaacctaaa caaccaggta agtacagaag aggtcaaatc
      481 tctcttatca gctcacttgg atccaaatag tgttgatgca ttttttaatc tcgttaatga
      541 ctataatacc attgtcggct caactggctt atcaggagat ttcacttcct ttactcacac
      601 cgaatacgat gttgagaaaa tcagtcatct ctggaatcaa aagaagggcg attttgttgg
      661 gaccaactgc cgtatcaata gttattgtct tttgaaaaat tcagtcacca ttccaaagct
      721 tgaaaagaat gaccagttgc ttttcctaga taatgatgcg attgataaag gaaaggtctt
      781 tgattcacaa gataaggaag agtttgatat tctattttcg agagttccaa ctgaggcaac
      841 tacagatgtc aaggttcacg ctgaaaagat ggaaacattc ttctcacaat ttcaattcaa
      901 tgaaaaagct cgaatgctgt ctgtagtctt gcacgacaat ctggatggcg agtatctgtt
      961 tgtaggccac gttggggtct tagtacctgc tgatgacggt ttcttatttg tagagaaatt
     1021 gactttcgaa gagccctacc aagcgattaa atttgctagt aaggaagatt gctacaagta
     1081 tttgggcacc aagtatgcgg attatacagg cgagggactg gctaagcctt ttatcatgga
     1141 taatgataag tgggttaaac tttaatagtg ttatgatatg acagtaatct taggattttt
     1201 atttctattt ttgatgagtt ggtgggttat ttcaatcgtt aatagccaga aaccacgcaa
     1261 aaatgaaaca tttattggct atgtccaaag atacgatatt gataggaatg gctatcaatc
     1321 attaccgtgt cctcagaact gagaggagtt cttgaaatcg taacaccact ttgaacaaga
     1381 gttacttcaa cccattggct ccgacggatt aagttgcttt cgtgaatacc aagatcagcc
     1441 gcaatttctt cataagtgcg gtattctcac acatattgaa gagtggccat aagaaggtct
     1501 tctaggctta atttaggttt tcgtccacct tttgcgtgtt taagttgata agctgttttt
     1561 aatac
//
LOCUS       DAVGJL010000194         1558 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.197, whole genome shotgun sequence.
ACCESSION   DAVGJL010000194 DAVGJL010000000
VERSION     DAVGJL010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.197"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..430
                     /locus_tag="VVE42_002010"
     CDS             <1..430
                     /locus_tag="VVE42_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEU3074140.1"
                     /translation="LIEDLETQRVVMQYRAPENNRWSGYAFPGGHVENDEAFAESVIR
                     EIYEETGLTIQNPQLVGIKNWPLDTGGRYIVICYKATEFSGTLQSSEEGEVSWVQKDQ
                     IPNLNLAYDMLPLMEMMEAPDKSEFFYPRRTEDDWEKKIF"
     gene            complement(427..942)
                     /locus_tag="VVE42_002011"
     CDS             complement(427..942)
                     /locus_tag="VVE42_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEU3074141.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQSLAEHFHVTRQVIVQDIAILR
                     ADGAPILSTNRGYIYKQIDTNPYVHKLFKVKHEVEEIGQELLAIVDNGGHVQNTLIDH
                     PVYGEIETLLKLSCRRDVQHFLEQVEHSDFRPLSELTDGVHYHLVEAETQQDLHYIEE
                     ALDQLGYLVKD"
     gene            complement(954..1481)
                     /locus_tag="VVE42_002012"
     CDS             complement(954..1481)
                     /locus_tag="VVE42_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU3074142.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAVFVILASSFGFLMAGYPMVIVFRAFSHISFGALGALYLQKFPDTL
                     DKPKSSWVFNFVLAVVHALAEVLACVLFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
BASE COUNT          485 a          303 c          345 g          425 t
ORIGIN      
        1 tctgattgaa gacctcgaaa ctcagcgcgt ggtgatgcag tatcgcgccc ctgaaaacaa
       61 tcgctggtct ggttatgcct ttcctggagg tcatgtagaa aatgatgagg cttttgcgga
      121 gtctgtcatt cgtgaaatct acgaagaaac agggttgact atccaaaatc ctcaacttgt
      181 cggcattaaa aattggccac tagatacagg tgggcgctat attgtcattt gttataaggc
      241 gactgagttc tctggtaccc ttcaatcttc agaagaggga gaagtttctt gggtgcaaaa
      301 agaccagatt ccaaacttaa atctggccta tgatatgcta ccattgatgg aaatgatgga
      361 agctcccgac aagtcagagt ttttctaccc tcgccgtaca gaagacgatt gggaaaagaa
      421 aatcttctag tcttttacta aataacctag ctgatccaag gcctcctcga tatagtggag
      481 gtcttgttgt gtttcggctt cgactaggtg gtaatggacg ccatctgtca attcagatag
      541 aggtctaaag tcagaatgct cgacttgttc tagaaaatgt tgcacatctc tgcgacaaga
      601 cagtttaagc aaggtttcaa tttctccata aacgggatgg tcaatcaagg tattctggac
      661 atgcccgcca ttatcgacaa tagcaaggag ttcctgaccg atttcttcaa cttcatgttt
      721 cactttgaaa agtttgtgaa cataaggatt ggtatcaatt tgcttataga tgtagccacg
      781 attggtggat aggataggag cgccatcggc tctcaaaatt gcaatgtcct gcacaatgac
      841 ctgacgtgtg acatgaaaat gttccgccaa actttggcca ttaagggctt taggagcttc
      901 tttcaagagt tggagcaggg cttgtttgcg atcttttgtc atagcttttc cttttaacgg
      961 cgttttcgaa gcactttata gacagctagt gctaatgtat agtctaccat actatggata
     1021 attgtgccaa atccaactag tacaaataga acatagaaca tgttttctac attggtaccc
     1081 gaagttgcgt aaaaaaggac acaggctaat acttcagcaa gggcatgaac aacagccaaa
     1141 acaaagttga aaacccagga agattttggt ttatctaggg tatcggggaa tttttgtagg
     1201 taaagagctc ctaaagcacc aaaagatata tgggaaaaag cccgaaaaac gataaccatg
     1261 ggatagccag ccatcaaaaa tccaaaacta gaggctagga tgacaaaaac tgccatcaag
     1321 ggcgacaaga acatggctat aaaaatagcg atgtggctcc ccaaagtata ggaagcaggt
     1381 ggaatgacaa tcttgaaagg cataacaatt ggaatcaaaa tcgcaatagc cgttaaaagg
     1441 gctgtcattg tcataaattg tgtctttttc cgtgtattca caagaatctc ctttttaact
     1501 gcatatacac tagtatggta caataaacca gacaataaag caagaattta cttgggtt
//
LOCUS       DAVGJL010000195         1472 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.198, whole genome shotgun sequence.
ACCESSION   DAVGJL010000195 DAVGJL010000000
VERSION     DAVGJL010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.198"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            144..1340
                     /gene="tuf"
                     /locus_tag="VVE42_002013"
     CDS             144..1340
                     /gene="tuf"
                     /locus_tag="VVE42_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEU3074143.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
BASE COUNT          463 a          312 c          300 g          397 t
ORIGIN      
        1 ttaaattttt accaagaata attcacaaaa acgttgtaaa acacttgcaa tttagctgaa
       61 atttgataaa atagtaagga aagttagact gtattgccta ctgtctatct ataaaatata
      121 ttttattgga ggcttttact caaatggcaa aagaaaaata cgatcgtagt aaaccacacg
      181 ttaacattgg tactatcgga cacgttgacc acggtaaaac taccctaact gcagctatca
      241 caactgtttt ggcacgtcgc ttgccttcat cagttaacca acctaaagac tatgcgtcta
      301 tcgatgctgc tccagaagaa cgcgaacgcg gtatcactat caacactgcg cacgttgagt
      361 acgaaactga aaaacgtcac tacgctcaca tcgacgctcc aggacacgcg gactacgtta
      421 aaaacatgat cactggtgct gctcaaatgg acggagctat ccttgtagta gcttcaactg
      481 acggaccaat gccacaaact cgtgagcaca tccttctttc acgtcaggtt ggtgttaaac
      541 accttatcgt cttcatgaac aaagttgact tggttgacga cgaagaattg cttgaattgg
      601 ttgaaatgga aatccgtgac ctattgtcag aatacgactt cccaggtgac gatcttccag
      661 ttatccaagg ttcagcactt aaagctcttg aaggtgactc taaatacgaa gacatcgtta
      721 tggaattgat gaacacagtt gatgagtata tcccagaacc agaacgtgac actgacaaac
      781 cattgcttct tccagtcgag gacgtattct caatcactgg acgtggtaca gttgcttcag
      841 gacgtatcga ccgtggtatc gttaaagtca acgacgaaat cgaaatcgtt ggtatcaaag
      901 aagaaactca aaaagcagtt gttactggtg ttgaaatgtt ccgtaaacaa cttgacgaag
      961 gtcttgctgg agataacgta ggtgtccttc ttcgtggtgt tcaacgtgat gaaatcgaac
     1021 gtggacaagt tatcgctaaa ccaggttcaa tcaacccaca cactaaattc aaaggtgaag
     1081 tctacatcct tactaaagaa gaaggtggac gtcacactcc attcttcaac aactaccgtc
     1141 cacaattcta cttccgtact actgacgtta caggttcaat cgaacttcca gcaggtactg
     1201 aaatggtaat gcctggtgat aacgtgacaa tcgacgttga gttgattcat ccaatcgccg
     1261 tagaacaagg tactacattc tctatccgtg agggtggacg tactgttggt tcaggtatgg
     1321 ttacagaaat cgaagcttaa ttcgatttag ttcccagaag aacaattatt taagtcagac
     1381 actaaaagaa tcttgcttgg caaggttctt ttttggctct ttgtcaactg tagtgggttg
     1441 aaaaaaaact aagctcgaga aaggacaaat tt
//
LOCUS       DAVGJL010000196         1466 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.199, whole genome shotgun sequence.
ACCESSION   DAVGJL010000196 DAVGJL010000000
VERSION     DAVGJL010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.199"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(562..1134)
                     /locus_tag="VVE42_002014"
     CDS             complement(562..1134)
                     /locus_tag="VVE42_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3074144.1"
                     /translation="MNKLMKFISVFLTSIVLIVSAIPSVSAVYASEQVSQIETNMELQ
                     PVTSLTEEQINTLANEIQSFHPDVSQQWIKEVINRQLQGDYTIPPTYSPFRAAWQGIT
                     VNQMGALLDTAIALALGGTTAGLANLIKVKGKHAAKSAIRSAISRYLGSWFVNDVALE
                     SAMNLLSPGTYLAQLWDKNDAIPNNGRINF"
BASE COUNT          437 a          244 c          242 g          543 t
ORIGIN      
        1 gcattgtcat ttatgtacta agttatttta agaatgtagg gaaataaacc ctacattctt
       61 tttagttttt tctgttttct aaattctatt tatccaagcg cttcaacatt tcttgcttct
      121 tcgcttcaag ttctgcacgc ttttcttcga tttcggcatg ttttttctcg agttcagaac
      181 aacttgcacc attgctaaat tcttttcgcc atcaggagat agggtgagtc gacatgtcta
      241 ttactcaccc aaagcagtcc tacaaagcag gaattttctg ttactttttt ggaaatagta
      301 acgtttatac agctttgaca cttcgtatca aagcgccaaa cacactccga gggatttaca
      361 gaaagcagaa aaggaatgat ctggtataag atcattcctt ttctctcttt ttctttaagt
      421 aattatatac aatgtacgac gaagtcgtca ttgcaatgct gatccaccac ctaaagggaa
      481 ctttaaacaa cattgataag ataaagaata taaacaacga aaatacgtta tacccaatta
      541 attttattgt atatctcatg attaaaagtt aatccttccg ttgttaggaa tggcatcatt
      601 tttatcccat aattgtgcta aataagtccc cggtgataat aaattcatag cggattctaa
      661 agcaacatca tttacaaacc aactacctag atatctagaa attgctgaac gaatagcact
      721 ttttgctgca tgttttcctt ttactttaat tagatttgca aggcctgcag tagttcctcc
      781 taatgctaaa gctattgcag tatctaatag agcacccatt tgattaactg taataccttg
      841 ccaagctgct ctaaatggag agtatgtagg tgggattgta taatcgcctt gtaattgtcg
      901 gttaattact tctttgatcc attgttgtga gacgtctgga tgaaaagatt ggatttcgtt
      961 tgcaagtgta ttgatttgtt cttctgttag agaagtgaca ggttgaagtt ccatatttgt
     1021 ttcaatttgt gatacttgtt cagaagcgta tacagctgaa acacttggaa tcgctgatac
     1081 aattaacaca attgacgtca aaaaaaccga aataaatttc attaatttgt tcatgagctt
     1141 ttctcctttt tatttgtatc tgcttacatt ttattatata ctgttattat agtcaagaaa
     1201 aatatgctat tatgttaaaa aaatattttt aaaaatataa atggacggat ttattttgga
     1261 ttttatttgt tattttgacc tgcctctata ttggtaacca tgatttgttt actttcaatc
     1321 atcaagaatt ctcttttcgt ggtagcgttt ggggtctggt actggcctta tatcacttat
     1381 tgttcattga taagtttgta atttcgaatc gaaaataaag attagaacta tgattgactg
     1441 gctactttta atattgattt tggagg
//
LOCUS       DAVGJL010000197         1427 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.200, whole genome shotgun sequence.
ACCESSION   DAVGJL010000197 DAVGJL010000000
VERSION     DAVGJL010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1427
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.200"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..347
                     /locus_tag="VVE42_002015"
     CDS             <1..347
                     /locus_tag="VVE42_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEU3074145.1"
                     /translation="EIIELGEEIAIGLGANPELSRLVLIFCAVSLTAFSTSITGPIAC
                     ISFLAGPIALNIGKKRSPILAGLVGILLVLLSDIFSQNILPARYPVGVVTGLLGSPYL
                     IYLLIKMNRRNI"
     gene            347..1141
                     /locus_tag="VVE42_002016"
     CDS             347..1141
                     /locus_tag="VVE42_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3074146.1"
                     /translation="MKGLWSNNLTCGYDEKIILENINIKIPEEKISVIIGSNGCGKST
                     LIKTLSRLIKPLEGEVLLDNKSINSYKEKDLAKHIAILPQSPIIPESITVADLVSRGR
                     FPYRKPFKSLGKDDLEIINRSMVKANVEDLANNLVEELSGGQRQRVWIALALAQDTSI
                     LLLDEPTTYLDISYQIELLDLLTDLNQKYKTTICMILHDINLTARYADYLFAIKEGKL
                     VAEGKPEDILNDKLVKDIFNLEAKIIRDPISNSPLMIPIGKHHVSS"
BASE COUNT          545 a          225 c          235 g          422 t
ORIGIN      
        1 tagaaattat tgaacttggt gaagaaatag caatcggact tggagcaaat cccgagcttt
       61 caaggcttgt tttaattttt tgcgctgtat ctttaactgc tttttctact tcaattacag
      121 gaccaatagc ttgtatatct tttttagctg gtcccatagc cttaaatatt ggcaagaaaa
      181 gaagcccaat attagctgga ttggttggaa ttttactagt tttgttatca gacatattct
      241 ctcaaaatat tttaccagct agatatccag taggtgttgt aactggcttg ttaggttcac
      301 catacttaat atatttacta ataaaaatga acaggaggaa tatataatga aaggtctgtg
      361 gtcaaataat cttacctgcg gttatgatga aaaaataatc ttggaaaata taaatataaa
      421 aatacctgaa gaaaaaatat cagttattat tgggtcaaat ggttgtggga aatcaacact
      481 cattaaaacc ttgtctcgac ttataaagcc attagaggga gaagtattgc ttgataataa
      541 atcaattaat tcttataaag aaaaagattt agcaaaacac atagctatat tacctcaatc
      601 tccaataatc cctgaatcaa taacagtagc tgatcttgta agccgtggtc gtttccccta
      661 cagaaagcct tttaagagtc ttggaaaaga tgaccttgaa ataataaaca gatcaatggt
      721 taaggccaat gttgaagatc tagcaaataa cctagttgaa gaactttctg ggggtcaaag
      781 gcaaagagta tggatagctc tagccctagc ccaagataca agtatcctac ttttagatga
      841 gccaactact tacttggata tctcatatca aatagaacta ttagacctct tgactgatct
      901 aaaccaaaaa tataagacaa ccatttgcat gattttgcac gatataaatc taacagcaag
      961 atacgctgat tacctatttg caattaaaga aggtaaactt gttgcagagg gaaagcctga
     1021 agatatacta aatgataaac tagttaaaga tatctttaat cttgaagcaa aaattatacg
     1081 tgaccctatt tccaattcgc ctctaatgat tcctattggc aagcaccatg ttagctctta
     1141 ataattgaat attggaattc atttaaaagt cgcaaatttt gaactgaccc tcaaaagtta
     1201 ggccaaaaaa ctaactaaag gaggtcagtt ttttaatggc aaatacagca cagaatttaa
     1261 gataaaatta gtaaaagaat atcatgaatg aaaaatagga tatagagaat aacagcaata
     1321 ttaaaaagat caggactgat aataaacaaa aagaaagtac aacgactagt tcaaaagcta
     1381 aaacttcaag taaaaagtta ttcaagaaaa tctagaaaat actcatc
//
LOCUS       DAVGJL010000198         1419 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.201, whole genome shotgun sequence.
ACCESSION   DAVGJL010000198 DAVGJL010000000
VERSION     DAVGJL010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.201"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            83..1192
                     /locus_tag="VVE42_002017"
     CDS             83..1192
                     /locus_tag="VVE42_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU3074147.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGGFLYYLTNPIVTFLNKVCKLNRLLGILITLCTLVWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSRLYENHKIMKERERELAK"
BASE COUNT          426 a          204 c          259 g          530 t
ORIGIN      
        1 aagaaaaaaa atattcttgc tttaataatt catctgttaa gctttttgaa aatatggtaa
       61 aataaagtga gggaggtaac tcatgtttcg tagaaataaa ttattttttt ggaccacaga
      121 aattttactc ttaaccatca tcttttacct gtggagacag atgggatctt tgattaaccc
      181 ttttgttagc gtgcttaata caattatgat tccattttta ttagggggct ttctttatta
      241 tttgacaaac cctattgtta ctttcttaaa taaagtctgt aaactcaatc gtttgcttgg
      301 tattttaatt accttgtgta ctttggtctg gggaatggtc ataggtgttg tctatctctt
      361 acctattttg attaatcagt tatctagttt gattatatct agtcaaacta tttatagtcg
      421 agtacaagac ttaatcatag acttatctaa ttatcctgcg ctccagaatt tggatgtaga
      481 agctacaatt cagcagttaa acttatccta tgttgatatt cttcaaaata tcctaaatag
      541 cgtatcaaat agtgtgggga gcgtcttgtc agctcttatc agtactgttt tgattttgat
      601 tatgactcca gtttttttgg tttatttctt attagatgga cataaattct tgcccatgct
      661 tgaaagaacg attctaaaga gggatcgctt gcatattgca ggcttattaa agaatttaaa
      721 tgcgacgatt gctcgctata ttagtggagt ttcgattgac gcaatcatta taggttgttt
      781 ggcttatatt ggctatagta ttattggttt aaaatatgct ttagtttttg ccattttttc
      841 tggtgtagcc aatttaattc cttatgtggg gccaagtatt ggtttgattc ctatgatcat
      901 cgcaaatata ttcactgatc cccatagact gctgattgca gtgatttata tgcttgttgt
      961 tcagcaggta gatggcaata tcttatatcc tcgaatcgta ggaagtgtta tgaaggttca
     1021 tccaatcacg attttagttt tacttttgtt gtcaagcaat atctatggtg tagttggaat
     1081 gattgtcgca gtgccaacct attctatctt gaaagaaatt tctaagttct tatcccgttt
     1141 gtatgaaaat cataaaataa tgaaagaacg agaaagagaa ttagctaagt aaaagtcagg
     1201 agaaccctga tttttcttta ctggaagtgg cctttagatt agaagactga aaataagtta
     1261 aagtcttaaa ctaattttca cagctaagaa tagtagaagt taatctgata aaaatcgaaa
     1321 aaaccagtgg aattctgtgt cagggtaagt tccactggtt ttcatagtct attaaagttc
     1381 gaatgaaacc tatttatagt agattgaaac tagaatagt
//
LOCUS       DAVGJL010000199         1394 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.202, whole genome shotgun sequence.
ACCESSION   DAVGJL010000199 DAVGJL010000000
VERSION     DAVGJL010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.202"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(<1..258)
                     /locus_tag="VVE42_002018"
     CDS             complement(<1..258)
                     /locus_tag="VVE42_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3074148.1"
                     /translation="MSNVDKIRKIHIIVCWVYIFLSFRAIINDTEYFLLIFLAFIYSI
                     VSLPLYSVKNKIVSICLAINSILLMSFPILINKFFPESFLTY"
     gene            complement(255..608)
                     /locus_tag="VVE42_002019"
     CDS             complement(255..608)
                     /locus_tag="VVE42_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEU3074149.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGAYLSKRACQGICVALAMSPGT
                     FIALAGAAVLTKKLINYIKVGGLGGWLIGAAAGVLATAAGKIAYCIGYGVLNRGCDIN
                     GNPYPWDGFISATVR"
     gene            complement(664..>1394)
                     /locus_tag="VVE42_002020"
     CDS             complement(664..>1394)
                     /locus_tag="VVE42_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3074150.1"
                     /translation="LYALKDLYKKEALITIFPFIFIGRKKVNVRFSPYFSFHRKSLSV
                     DDLKSRMIWSFILEIAIILVFILKIPFAIIMLTTIFFWNIMDINHLVFNKTEFLFNQN
                     KWQKEDSFESDLTKTLKDKIQKSELSYSDLMSLQLYDAMNQSTFLTDSELFEDILKKI
                     EDSHNTLLCTGLVELLLYEISISKNHNWSEKIDKIRIELIRINQLDFFYYTSWLRQNF
                     DFCMNREYHKMKSRKLLLSNKKIV"
BASE COUNT          490 a          258 c          183 g          463 t
ORIGIN      
        1 ataagtcaaa aaactttctg ggaaaaactt attgattaaa atggggaaac tcattaacag
       61 aatcgaattt atggctagac agatagatac tattttattt ttcacagaat atagaggtag
      121 tgatactatt gagtagataa aagctaaaaa aataagcaaa aaatactccg tatcatttat
      181 aatcgccctg aatgataaaa atatatacac ccaacacacg atgatgtgta tttttcgaat
      241 cttatctaca ttactcatct tactgttgca gatatgaatc catcccaagg ataagggttc
      301 ccgttaatat cacaacctct attaagaaca ccatatccaa tacagtaagc tattttccct
      361 gctgctgtag ccaatacacc tgctgctgca ccaataagcc agcctccaag acctccaacc
      421 ttaatatagt ttattagttt tttggttaaa actgcagctc cagctaatgc tataaaagtt
      481 cctggactca tagctaaagc tacgcaaatt ccttgacaag cacgcttgct taaataagct
      541 cccccatcaa gatacatcat ttcttcttca tcaatcacaa cataattatt tggtaataca
      601 agttccattt gttttctcct ccttagttga aattgtttca tttgttttac aaataggacg
      661 aacttaaact atctttttat tactcagtaa taatttccga gatttcattt tatgatactc
      721 tctattcata cagaaatcaa aattttgcct taaccaactt gtataataga aaaaatctaa
      781 ctgattgatt ctgataagct ctatcctaat tttatcaatc ttttctgacc aattatgatt
      841 cttactaata gaaatttcat acagcagtaa ctcgacaaga cctgtacaca gaagggtgtt
      901 atgagaatct tcgatttttt tcaaaatatc ctcaaaaagt tcactatccg ttaaaaaggt
      961 ggattggttc atcgcatcat acagctgaag agacattaag tcagaataac ttagctctga
     1021 tttttgtatc ttatccttta acgttttcgt caaatcgctt tcaaatgaat cttctttttg
     1081 ccatttgttt tgattaaaaa gaaattccgt cttattaaaa acgagatgat tgatatccat
     1141 tatattccaa aaaaatattg tagtaagcat gatgatcgcg aaaggaattt ttaaaataaa
     1201 tactaggata atagcaattt caagaataaa actccatatc attctagatt ttaaatcatc
     1261 aaccgacaaa ctttttcgat ggaatgaaaa atagggcgag aacctcacat ttactttctt
     1321 cctgcctata aaaataaaag gaaaaatcgt aataagagct tctttcttgt acaaatcttt
     1381 caatgcgtat agta
//
LOCUS       DAVGJL010000200         1391 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.203, whole genome shotgun sequence.
ACCESSION   DAVGJL010000200 DAVGJL010000000
VERSION     DAVGJL010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.203"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            223..360
                     /locus_tag="VVE42_002021"
     CDS             223..360
                     /locus_tag="VVE42_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapB/AmfS family lantipeptide"
                     /protein_id="HEU3074151.1"
                     /translation="MAAEVLNLQLVSVQVDETDEVDGMRFSTFSTNRCGNWSAFSWEN
                     C"
     gene            1169..1375
                     /locus_tag="VVE42_002022"
     CDS             1169..1375
                     /locus_tag="VVE42_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001828718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin transporter"
                     /protein_id="HEU3074152.1"
                     /translation="MYDLKQMGNKGGCTIQLIHHFPFGMGLGHLKKDYIEFKRVGIFD
                     GKVVEVTLREPYSRDLLQVVKSIK"
BASE COUNT          448 a          183 c          274 g          486 t
ORIGIN      
        1 aattttatag tatctattat tttatttatt ttaatattta ttactaaaag ttcctatttt
       61 atctttaaga atgcaaaaat tatttcttaa aaatttgtaa gttttcgcta tataaaatag
      121 ttaaatctat tttgtatcat tgcttgcatt ttactggtgg agagagtata tgagggtgga
      181 taaagtactt aatcccaaaa atttaaatag gaggagctag ctatggcagc agaagtatta
      241 aatcttcaat tagtatctgt tcaagttgac gaaacagatg aagttgatgg aatgcgattt
      301 agtacattca gtacaaaccg ttgcggtaac tggagtgctt ttagttggga aaattgttaa
      361 ttcatttaaa ttgtaaactt tagagattct ctggagttat ggatgtcgtt ccaatatgtg
      421 cacgttggaa tgttagtgct tatatggggg gaacagaatc ctctcttgat tgaagacaag
      481 ctagtcatta ggctggtttg tctttttgtc aactgtagtg ggttgatata atagtattag
      541 tgagtgggat aaaagtttca tttagtttat tcagtacaaa tttaacgggt caagatttat
      601 atactggtgg tgtttttggg gctgagagaa gcatcttgat tttatgtgtg gtttttatac
      661 ttacagttgt tctgctccaa agagcttgta gagaagaatt agctcataaa ggagattgat
      721 tattttgata tcaaaaaaat gcacaggata acctgatgca tttttttagc gacaatgctt
      781 gctacttcct tctgtcgaat ttagacaatt ttaaataaaa gaatgaaata ttccctactg
      841 tatttttaga cgaagaacgt tagttaataa tactcaaatt tatttcaaac tctttgatta
      901 gtatgaattt agctctacag aaaaataaaa aatgtgataa aaatttcaac tactcttatc
      961 tataataaag ctacctgttc tttgaaaatt gaatccactc cgtcttgatt agcgtgcctg
     1021 tgtaagtagg gactgagagt atttccctgt gaagggcaac tggcacaaga cgtgtgtgag
     1081 aattttctaa cagacacgga gtttatcgtt tccagactca aacgatgcga caaactagat
     1141 aaaggagtta atcatgaagg tagtaaactt gtatgatttg aaacaaatgg gaaataaagg
     1201 tggttgtacc atccaattga ttcatcactt tccttttggt atgggcttag gacatctcaa
     1261 aaaagactac attgaattta aacgtgttgg tatctttgat gggaaagtag tagaagttac
     1321 tcttcgagaa ccttattcga gagatctact tcaggttgtc aagtcaataa agtaactgac
     1381 accacgagta t
//
LOCUS       DAVGJL010000201         1323 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.204, whole genome shotgun sequence.
ACCESSION   DAVGJL010000201 DAVGJL010000000
VERSION     DAVGJL010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.204"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            48..>1323
                     /locus_tag="VVE42_002023"
     CDS             48..>1323
                     /locus_tag="VVE42_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3074153.1"
                     /translation="MLVDFITNSRKQYSFINVDGISVEDISDDSNLKFYYYGQEKGEI
                     FNYQEENDLFIYYKNPLTGIEVPLENTIKVVETYEETDLIITIGNTIFQNVKRHRFPD
                     GSVQLHFGEGFKMSFDVKKKQFTFNYTRPNMLSKAIKCTQAFQELGKFGYCKLNGNII
                     ELGEQSILDITSRDLETEIEELVQISNFMENMRIQKEVDLTYFDKQSLRNLNILNLGL
                     ILKNKVALNYNESKLLHLRIANIHIITLYDFETDNIGTMIDIFTETPWCRRGEGEDSS
                     YISIFEVLEPNDWLKIDNCDFDSVISSYQRLVDNGLKFEDANNTIIKIVIAADKAEDV
                     SRSELLLNWAQCLSDWNLKYSKNCEMAIINDLQIKSRVRKLNSKEMEILSNILVNSND
                     NYELCFGLSVLLKSKPQADLFWNKLDNETKESY"
BASE COUNT          500 a          178 c          225 g          420 t
ORIGIN      
        1 aactttctgt acagattcat gaattagacg aacaaaaact gtaccctatg ctagtcgatt
       61 ttataacaaa tagtcgtaag caatatagct tcataaatgt tgatggtatt tcagttgaag
      121 atatctctga tgatagtaac cttaaattct attattatgg tcaagaaaaa ggagagatct
      181 ttaactatca ggaagaaaat gatctcttca tttattataa aaatccttta actggtattg
      241 aagtaccact tgaaaataca ataaaggttg tcgaaactta cgaggaaaca gatttaatta
      301 ttacaatagg gaatactatt tttcaaaatg taaaaaggca tagattccct gatgggagtg
      361 tacaactcca ttttggtgaa ggttttaaaa tgtcatttga tgttaagaag aaacaattca
      421 catttaacta cacaagacct aatatgttat caaaagccat aaagtgcact caggcatttc
      481 aagaactagg aaaattcgga tattgcaagc tcaatggtaa tataatagaa ctaggtgaac
      541 agtcaatatt ggatattact tcacgtgatt tagagactga gatagaggaa ttggtacaga
      601 tttcaaactt catggaaaat atgaggatac aaaaagaggt tgatctaact tactttgata
      661 aacaatctct aagaaatcta aatattctca atttaggcct tattctaaag aataaagttg
      721 ccttaaatta taatgaatca aaattacttc acctaagaat tgctaatatc cacattatta
      781 ccttatatga ctttgagaca gataatattg gaactatgat agacattttt actgaaactc
      841 cttggtgtag gagaggtgaa ggtgaagatt cgtcctacat ttcaatcttt gaagttttag
      901 aaccaaatga ctggctaaaa atagataatt gtgattttga ttcggttatc tcctcttacc
      961 aaagattagt tgataatggc ttgaaatttg aagatgctaa caacactatt ataaaaatag
     1021 ttattgctgc tgataaggca gaagatgttt ccagaagtga gttattgctt aattgggcac
     1081 aatgtctttc tgactggaac ttaaaatact caaaaaattg tgagatggct ataataaatg
     1141 accttcaaat aaaatctaga gttcgtaaat taaacagcaa agaaatggag attctgagta
     1201 acattttagt aaatagtaat gataactatg aactttgttt cggtttatca gtgttattaa
     1261 aatcgaaacc acaagctgat ttgttctgga ataaacttga taatgagaca aaagaaagct
     1321 ata
//
LOCUS       DAVGJL010000202         1320 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.205, whole genome shotgun sequence.
ACCESSION   DAVGJL010000202 DAVGJL010000000
VERSION     DAVGJL010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.205"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            89..616
                     /locus_tag="VVE42_002024"
     CDS             89..616
                     /locus_tag="VVE42_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HEU3074154.1"
                     /translation="MINEEISKEAGQAAQTIISYTIKATKESINLEKEIRKKMNETLE
                     KANGNLKSLMGDEMKIKDLYKKGQLENISIDQIDLKDLKKELNKLGVSFSVMKNKESK
                     NYEIFFQAKDIKVMEYAFKQVIAKENKKEKESILKQIKKYKDLSKNKDKTKEKGKRKV
                     KEKVKPNKKDMTREI"
     gene            743..>1320
                     /locus_tag="VVE42_002025"
     CDS             743..>1320
                     /locus_tag="VVE42_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HEU3074155.1"
                     /translation="MATNKRYYWIKLKEEFFTDKRIKRLRRISGGDTYTIIYLKLLLL
                     SLKDEGKLYYDGVESDFIKELALTIDETDDDVMVTVNYLINQGLLEVVTENDEYYLTE
                     IPNLIGSETAWAEKKRRYRQNKQRTLSLMSPTHVRQEIEIEKDIEIDKEREGEIDKKS
                     TPIFYGEFKNVRLSKEEYKNLKEKLNSHTDIM"
BASE COUNT          668 a          180 c          221 g          251 t
ORIGIN      
        1 aacaataaaa taaaaacaag caaagggaag tatagaaaaa ttaaaaccta tacttccctt
       61 ttttaattca aacaaaggag agaaaagcat gataaacgaa gaaataagca aggaagcagg
      121 tcaagcagca caaaccataa tatcatacac aataaaggca acaaaagaat caatcaattt
      181 agaaaaagaa ataagaaaaa agatgaatga aactttagaa aaagcaaatg gaaacttaaa
      241 aagtcttatg ggcgatgaaa tgaaaataaa agatctctac aagaaaggac aactagaaaa
      301 tataagcata gatcaaatcg acctcaaaga cttaaaaaaa gaactaaaca aacttggagt
      361 aagtttctca gtaatgaaaa acaaagaaag caaaaactat gaaatattct tccaagccaa
      421 agacataaaa gtaatggaat atgcctttaa gcaagtcata gccaaggaaa ataaaaaaga
      481 aaaagaaagt atcctaaaac aaataaagaa atacaaagac ctatccaaaa acaaagataa
      541 gacaaaagaa aaaggaaaaa ggaaagtaaa agaaaaagta aaaccaaaca aaaaagatat
      601 gaccagagaa atctaaaggg agtaacgaaa agttacaccc ttaaataacc ataataacaa
      661 gacttccgaa aaacagaagt ccagaattcc gaaaatcgga aatcaagaat ttcgattatc
      721 gaaaatcaag aaaggagcag atatggcaac aaacaaaaga tactattgga taaaattaaa
      781 agaagaattt ttcacagaca aaaggataaa aaggttaagg agaatatctg gaggagatac
      841 ctatacgata atctacctaa aacttctact actaagccta aaagatgagg gcaaactcta
      901 ttatgacgga gtagaaagtg attttataaa agagttagca ctaaccatag atgaaacaga
      961 cgatgatgta atggtaacag taaattattt aatcaatcaa ggcttattag aagttgtaac
     1021 agaaaatgat gaatactacc taactgaaat accaaactta atcggatcag aaacagcctg
     1081 ggcagagaaa aaaagaagat acagacaaaa taaacagagg acattgtcct taatgagtcc
     1141 aacccatgtc cgacaagaga tagagataga gaaagatata gagatagata aagagagaga
     1201 gggagagata gataaaaagt ccacccccat tttttatgga gaattcaaaa atgtaaggtt
     1261 aagcaaagaa gaatataaaa atctaaaaga aaaattaaac tcacacacag atataatgat
//
LOCUS       DAVGJL010000203         1265 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.206, whole genome shotgun sequence.
ACCESSION   DAVGJL010000203 DAVGJL010000000
VERSION     DAVGJL010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.206"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(6..680)
                     /locus_tag="VVE42_002026"
     CDS             complement(6..680)
                     /locus_tag="VVE42_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase"
                     /protein_id="HEU3074156.1"
                     /translation="MLHPIFIIRRSWDGIFHLSEWKRNEEFDFFNKMDVPYLSMSSRI
                     EVTQAINFHKKHSISLYAIISWCVMSAINSIPELLMDTDGKIVWQYNQRGCSFTTLTS
                     EDKLNFSSFTMGDNLIEFVSAFNINKQKAEEGQKPNIDKNNIAYLSCVPWIDFLHVST
                     PMNLSKIDTVPRITWGKVIQENQRYFCTVNLQINHGMGDGLHVSNFFVLLQRFVNKIN
                     EYFQKK"
     gene            complement(739..1142)
                     /locus_tag="VVE42_002027"
                     /pseudo
     CDS             complement(739..1142)
                     /locus_tag="VVE42_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          427 a          243 c          174 g          421 t
ORIGIN      
        1 aatcattact ttttttgaaa atattcattt attttattta caaacctttg caataacaca
       61 aaaaaattag atacgtgtaa tccatctccc attccatgat taatttgtaa attcactgta
      121 caaaaatatc tttggttctc ttgaataact tttccccacg ttattcgagg aactgtatca
      181 atttttgaaa gattcatagg agtgcttaca tgtaaaaaat caatccatgg aacacaagaa
      241 agataagcaa tgttattctt gtcaatattt ggtttttgcc cttcttctgc cttctgttta
      301 tttatgttga acgcagaaac aaattcaatt aaattatctc ccatcgtaaa tgaagaaaaa
      361 ttaagcttat cttccgatgt aagggtagtg aaagaacaac ctctttgatt gtattgccag
      421 acaatctttc cgtcggtatc cattagtaat tctggaatag aatttattgc actcataaca
      481 caccacgata ttatggcata aagtgatatc gaatgttttt tatgaaaatt gatagcctgc
      541 gttacttcta ttcgtgaact catagataaa taaggaacat ccattttatt gaaaaagtca
      601 aattcttcat tccttttcca ctctgataaa tgaaaaattc catcccaact cctccttata
      661 ataaaaatag ggtgtagcat tcttttttca tgctacaccc acaatcaacc atctttaagg
      721 cttactctga caagtaagtt aataagaatc tggactccaa gaacctgaag tatgaattct
      781 tacatgattt ccaaattgtg gcgccatagc taatctagta ccagaaccaa tataattgtc
      841 accacctcca ttatagtaca tgacaatcct agagccagac cccaatgaat ataccggggt
      901 aatatctgac ccactatagg cgctacgaat agaggtattt aaccttttac cgccaccagt
      961 gctgtcatta ttattaattc cagcagaggc gttttctttc tcaagctcac tagttgtatc
     1021 cgaaccccaa cgcggcaacg ttaaatcata atttccaatc tgtctagcta aaactgtagg
     1081 gagaaacaaa ctagatgcaa ttgtaactac gcaaaaaagg aatatatttt tcttcttttc
     1141 atgatatcgt cctcctaaga agtgataaac aagatagttg attgatcaat ttgatacata
     1201 ttacattttc attatatcaa taaaagaaag cgttgtcaat cattttattt aaaatattgt
     1261 catat
//
LOCUS       DAVGJL010000204         1239 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.207, whole genome shotgun sequence.
ACCESSION   DAVGJL010000204 DAVGJL010000000
VERSION     DAVGJL010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.207"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..190
                     /locus_tag="VVE42_002028"
     CDS             <1..190
                     /locus_tag="VVE42_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3074157.1"
                     /translation="NFSGQKKLIEQLYRYLCVIEEGFVIAGDNGLKITTDIASGFAGV
                     AIGLVSIMDNKLTILPQI"
     gene            223..>1239
                     /locus_tag="VVE42_002029"
     CDS             223..>1239
                     /locus_tag="VVE42_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEU3074158.1"
                     /translation="MNQSNEIFLNTIQEKTHYKRSISSSFQELKKWNSLIFRRGRYWY
                     QFDNWSIPNTGVIKEWYVEDNDWIPKVSSSSLDNSNSLKIVFDVEKYTLPDDSILQDV
                     RISSDESYIVLVVSSLRSTNLIGIKKYTMEKLFIIEDISVESSFYLGKFGVMYTRSKE
                     YGRPSKLFYKSFDSFTEEELFEENECSFRLKIVHIDSNNCFVKSVDFQKGRIFLYSFD
                     RTGFVRHSYTETVAPTPRDIALFSTEKAQYFLGLSSTEEKKDQLILRETSSGDRVSIT
                     IPYQDRARRVHCIGRYILLDCSNAANSVFYLATFKNNSLRELSVNKITFDEKTTLYEN
                     SFSDR"
BASE COUNT          423 a          167 c          240 g          409 t
ORIGIN      
        1 taacttttca ggacaaaaga aattaattga acaactttat agatatttgt gtgtaattga
       61 agaaggattt gttattgcgg gagataatgg tttaaagata acaacagata ttgcttcggg
      121 atttgcgggt gttgcaattg gattggtatc aataatggat aataaattga cgattcttcc
      181 tcaaatataa tatttagata ccattattaa ggggtcatac ttgtgaatca gagtaatgaa
      241 atatttttaa atacaataca ggaaaaaaca cactataaga ggtctatctc ttcatcattt
      301 caggaattaa aaaaatggaa ttctttgata tttaggagag ggcgatattg gtaccaattt
      361 gataattgga gtatacccaa tactggtgta ataaaggaat ggtatgtcga agataatgac
      421 tggattccta aggttagttc ttccagttta gacaattcaa attcacttaa aattgtcttt
      481 gatgtggaaa aatatacttt gccagatgat agtatcttac aggatgttag gatttcatct
      541 gatgagagtt atatagtttt ggttgtatcc tcactgcgta gcactaattt aattggaata
      601 aaaaaatata ctatggagaa gttatttatc attgaagata tttcagtaga aagttcgttc
      661 tatttgggta aattcggtgt tatgtatact cgctccaaag agtacgggag gccaagtaag
      721 ttattctata aatcttttga tagctttaca gaagaggaac tgtttgaaga aaacgaatgc
      781 tcttttcgat taaaaatagt ccacattgat agcaataatt gttttgtaaa gtcagtagac
      841 tttcagaaag gcaggatttt tctatacagt ttcgaccgga cgggttttgt tagacactct
      901 tacacagaaa cggtagcccc aacacctaga gatatcgctc tatttagtac agaaaaagca
      961 cagtactttc ttgggctctc ttcaactgaa gaaaagaaag atcaactaat tctaagggaa
     1021 acttcttcgg gagacagagt ttctattact ataccatatc aagatagggc tagaagagtt
     1081 cactgtatag gacgttatat ccttttagac tgttcaaacg cagcgaattc cgtcttttat
     1141 cttgctacat ttaaaaataa tagtttgagg gagttgagtg ttaataagat aacatttgat
     1201 gaaaagacaa cactttatga aaattcattt tctgatcgt
//
LOCUS       DAVGJL010000205         1229 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.208, whole genome shotgun sequence.
ACCESSION   DAVGJL010000205 DAVGJL010000000
VERSION     DAVGJL010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.208"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            31..>1229
                     /locus_tag="VVE42_002030"
     CDS             31..>1229
                     /locus_tag="VVE42_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEU3074159.1"
                     /translation="MITKQSAFLQNRATILEFLYRNPATSRTDIVNETGLTPATTTNI
                     IKELSEQSLIYETGDEFSEFSGSGRRRKTISITDNIPYVVGGIEINVLGIFLSLCDLQ
                     GKTLFETEILNEDYPISEINSTITNMIKTAIEYVPLETKLLGFGLSIPGHYNKDSGSI
                     ITNNSIWESFNLLNVIKRFNFPFIVKNNIDCMAIGQYLFNPHNTPDNFIFLHAGLGIY
                     TSFFTKEKIGASKNPYIGEIGHTIVELNGQYCECGKKGCLQTYISDAWLIKHAQLLFK
                     NSQLTVLKSLVKTEKDIHLDTLLTAYNLGDSALRQQIDKGVNLLATSIANLLLINPAD
                     KIYINSQLLNYQPFTHEVRDKIQDQLHFVPFTRNIEIEILPYNKHRGSIGACALAIVA
                     FFIEHSNV"
BASE COUNT          441 a          234 c          182 g          372 t
ORIGIN      
        1 taatataaaa taaaccaaag gaggaaacaa atgattacaa aacaatcagc ctttttacaa
       61 aatagagcca ctatactgga gtttctatat cgaaatccag caacatcccg tacagatatt
      121 gttaatgaaa caggacttac tccagcaaca acgactaaca tcataaaaga gctaagcgaa
      181 cagtccctca tatacgaaac tggtgatgaa tttagcgagt tttccggatc tggaagacgt
      241 agaaaaacta tttctatcac agataatatt ccgtatgttg ttggaggtat tgaaataaac
      301 gtcctaggta tctttctcag tctatgtgac ttacaaggga aaactctttt cgagacagaa
      361 attttgaatg aagattatcc tatttcagaa atcaattcca ccattaccaa tatgataaaa
      421 acagctatag agtacgtccc tttggaaaca aaattacttg gatttggctt atcaatacct
      481 ggacattata acaaagactc cggaagtatc attacaaaca actccatatg ggaatctttt
      541 aatttattaa atgtaattaa aagattcaat tttcctttta ttgtaaaaaa taatatcgat
      601 tgtatggcta taggacaata cctttttaat ccacacaata cccccgataa ctttattttc
      661 ctacacgctg gattaggtat ttacacttcc tttttcacaa aagaaaaaat aggagcctct
      721 aaaaatcctt atatcggaga aattggacac accattgtcg aattgaatgg gcaatattgt
      781 gaatgcggaa aaaaaggttg tttacaaaca tatatttcgg atgcttggtt aatcaaacac
      841 gcccaattat tatttaaaaa ttcccaacta actgtactaa aaagccttgt aaagactgaa
      901 aaagacattc atttagacac ccttttaacg gcttataatt taggcgactc cgctttacgc
      961 caacaaattg ataaaggagt caatttattg gccacttcta ttgcaaatct tctcctcatc
     1021 aatcctgctg ataaaatcta tatcaacagt caattgctta attatcaacc tttcactcat
     1081 gaagtcaggg ataaaatcca agaccagctc cacttcgttc cctttactcg taatatagaa
     1141 attgaaattt taccttacaa caaacatcgt ggaagtatag gagcttgtgc attagctatc
     1201 gttgcttttt tcatagagca tagcaatgt
//
LOCUS       DAVGJL010000206         1192 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.209, whole genome shotgun sequence.
ACCESSION   DAVGJL010000206 DAVGJL010000000
VERSION     DAVGJL010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.209"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(9..206)
                     /locus_tag="VVE42_002031"
     CDS             complement(9..206)
                     /locus_tag="VVE42_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU3074160.1"
                     /translation="MTLNEYILQYRLKQAIDKMAESPNSPLSAISDQVGFSDYKYFAK
                     VFKKYLHISPKKLKSLGRIVK"
     gene            complement(281..1057)
                     /locus_tag="VVE42_002032"
     CDS             complement(281..1057)
                     /locus_tag="VVE42_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEU3074161.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGEEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEHHIHTAIETTAFVDHEKFIDLIQYVDFIYTDLKHYNSIKHKKVTGVFN
                     QMIIKNIHYAFSQNKTIVLRIPVIPNFNNSLEDAEKFATLFNSLNIDQVQLLPFHQFG
                     ENKYRLLNRKYEMDGVNALHPEDLIDYQKVFLNHHINCYF"
BASE COUNT          392 a          172 c          202 g          426 t
ORIGIN      
        1 agaatagcct attttactat tcttccgagt gatttcaatt tctttgggga aatatggaga
       61 tactttttaa atactttggc aaaatattta tagtctgaaa atccaacttg gtcagagata
      121 gcgcttaaag gggagttggg agattcagcc atcttatcta tagcttgttt caggcgatat
      181 tgtaagatat attcatttag agtcatgggg aagtcttctt tgataacctt atatagatgc
      241 ttgttatctg caaatagcta gagcatttca aggaaataaa ctagaaataa caattaatat
      301 ggtggttcag aaataccttt tgataatcaa taagatcttc aggatgaagt gcgttgactc
      361 catccatttc atatttccga tttaataaac gatatttgtt ttcaccaaat tgatgaaaag
      421 ggagtagttg aacttggtcg atatttaatg agttaaatag agtagcgaat ttttctgcat
      481 cctctaaact attgttaaaa ttaggaataa ctgggattct taaaacgata gttttatttt
      541 gagaaaaagc ataatgaatg tttttaataa tcatttgatt aaaaacccca gtcacttttt
      601 tatgttttat agaattataa tgttttaggt ctgtgtagat aaaatccaca tattgaatta
      661 aatcaataaa tttttcatga tcaacaaagg cagtagtttc aatggcagtg tgtatgtgat
      721 gttctttagc tgattttaag atggctttag caaattcaaa ctgagcaaat atttcacctc
      781 ctgataaagt taaacctcca ccggattctt cgtaaaattc tttgtctttt aatacctctg
      841 taataatttc ttctacagtc ttttcttcac cgactaaggt gaatttctct cgttgagcat
      901 ctttcatttt ttcaggtttc attctttgag attcaggatt agaacaccat ggacagcgca
      961 gaggacatcc ttttaaaaaa acagttgtac gaatacccgg gccgtcatga attgaaaagt
     1021 gttgaatatt aaaaataatt cctttagata tttccataag agttcctcct cttcataagt
     1081 ctatcctatc acaaaaacga aagaaaaaca attacgaacg aaagtttaat ttgttgtttt
     1141 ttgcttttta ttttggtaga ataaaacgga ggtgtaaaat gaaaagattg ga
//
LOCUS       DAVGJL010000207         1185 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.210, whole genome shotgun sequence.
ACCESSION   DAVGJL010000207 DAVGJL010000000
VERSION     DAVGJL010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.210"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..>1185
                     /locus_tag="VVE42_002033"
     CDS             <1..>1185
                     /locus_tag="VVE42_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HEU3074162.1"
                     /translation="TRKRIIFPSIATLEDIISRCRDKAENNLFSILLCSLTDIQIEKL
                     ESLFQIYEETKITKLAWLKDIPGKANPESFMSICKKVEVIASMGLGTINVSHINRNRF
                     LQLARLGENYDAYDFSRFELEKRYSLLIAFLVNHHQYLIDQLIEINDRILASIKRKGT
                     RDSQEQLKEKGKLATKKLEHYASLIDALHFAKDNDSNPFDEIERIMPWEDLVQDGEEA
                     KKITGNKNHGYLEMVRNKANYLRRYTPMLLRTLSFKATPAANPVLMALTQLTDLHNSG
                     KRKIPADTSTDFVSKKWKSLVRPEEGKIDRSYYELVAFTELKNNIRSGDISVEGSMIH
                     RNIDDYLVDLSACIDSETIPDTFEDYLKDREIILDLQLQFYSTVDKRISRANLKKLEK
                     VTP"
BASE COUNT          433 a          190 c          217 g          345 t
ORIGIN      
        1 aactcgaaaa agaattattt ttccatctat agctacactt gaagacatta taagccgctg
       61 tcgagataaa gcagaaaaca acttattttc aatattactc tgttcattaa cagatataca
      121 aattgaaaaa ctagagagtt tgtttcaaat ttatgaagag acgaaaataa ctaaactcgc
      181 ttggctaaaa gacattccag gtaaggcaaa tccagaaagc tttatgagta tttgtaaaaa
      241 agtggaagtg attgcttcca tgggactcgg gacaattaat gtctcccata ttaatcggaa
      301 caggtttctt cagctagcta gactagggga aaattatgat gcatatgact tctcccgttt
      361 tgagcttgaa aaaagatact ctttacttat tgctttttta gtcaatcatc atcaatatct
      421 gatcgatcaa ctgattgaga ttaatgaccg cattttagca agtattaaac gcaaagggac
      481 acgtgattca caagaacagt taaaagaaaa aggaaaattg gctactaaaa aattggaaca
      541 ttatgcttct ttaattgatg ctcttcactt tgcaaaagat aatgatagta atccttttga
      601 cgaaattgaa cgaatcatgc cttgggaaga tttagtacaa gatggagaag aagctaaaaa
      661 aattacaggt aataaaaatc atggctattt agaaatggtt cgaaataaag ctaattacct
      721 ccgaagatac acgccaatgt tattgaggac cctttcgttc aaagcaactc cggcagcaaa
      781 tccagtcctc atggccctaa ctcaactaac tgatttacac aatagtggta aaagaaaaat
      841 accggcagat acttctactg attttgtgag taaaaaatgg aaaagccttg ttcggccaga
      901 agaggggaaa atagatcggt cttactatga gttagtagct ttcaccgagc taaagaacaa
      961 tattcgatca ggagatattt cagttgaagg aagtatgatc catcgaaata ttgatgatta
     1021 cttagttgat ttatctgctt gtattgattc agaaactatt ccagacacgt ttgaggacta
     1081 tttaaaggat cgggaaataa ttttagattt acagcttcaa ttttattcga cagttgataa
     1141 gagaatttca agagcaaacc ttaaaaagtt ggaaaaagtt acacc
//
LOCUS       DAVGJL010000208         1178 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.211, whole genome shotgun sequence.
ACCESSION   DAVGJL010000208 DAVGJL010000000
VERSION     DAVGJL010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.211"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            100..948
                     /locus_tag="VVE42_002034"
     CDS             100..948
                     /locus_tag="VVE42_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU3074163.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDL
                     AKFGYVNLVPQIKQSANYDKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAN
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQTASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSQLTEITIEAEI
                     LVGKAF"
BASE COUNT          376 a          273 c          185 g          344 t
ORIGIN      
        1 tcattctata aattggagaa ttagttttat ttctactgaa tctcccatct acttattcct
       61 aaagttgctt tcatttgcct cttttgatac acttaaacta tgaatacaaa tctcaagccc
      121 aaacttcagc gttttgcttc tgcgactgcc tttgcctgtc ctatctgtca agaaaatctg
      181 actctgttag agactaattt caagtgctgc aaccgtcatt cttttgactt ggcgaaattt
      241 ggctatgtca atctagtccc tcaaatcaag caatctgcta actacgacaa ggaaaatttt
      301 caaaaccgtc aacaaatcct agaagccggc ttttaccaag ctatcttaga tgctgtatct
      361 gacttgcttg caagctcaaa aactaccaca acaattttgg atatcggttg tggtgaagga
      421 ttctattctc gcaaactaca agaaagtcac tctgaaaaaa ctttctatgc ctttgacatc
      481 tccaaagatt cagtccaaat cgctgctaaa agtgaaccca actgggcagt caattggttc
      541 gttggcgact tggcacgact tcctataaaa gacgctaaca tggatattct gcttgatatc
      601 ttctcacctg ccaactatgg agaatttcgt cgcgttttat ccaaagacgg tatcttgata
      661 aaggttatcc caactgaaaa tcacctcaaa gaaatccgtc aaagagtaca ggaccagctg
      721 acaaacaagg agtattctaa ccaagatatc aaggagcatt tccaggaaca ctttaccatc
      781 ctatctagtc aaactgcctc tctgactaag actatcacag cagaacaact ccaagcccta
      841 ctcagtatga ctcctctcct ctttcacgtt gaccagagca agattgactg gagccaactg
      901 acagagatta ccatcgaagc agagattttg gttgggaaag cattctaaac tagacttccc
      961 aaaggtataa aacttaccga aaatcttcaa tattacaaaa acgcataaga acaggtacac
     1021 aaaccttaat cttatgcgtt ttgttatagt ttccatacac attaagggga aatagttgaa
     1081 atcagttctc ttaataacca ttgaatacca tatactacat ttaccccaaa tatgatatag
     1141 gccgtataaa tcaagtattt cgatttctcc cgtctcaa
//
LOCUS       DAVGJL010000209         1161 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.212, whole genome shotgun sequence.
ACCESSION   DAVGJL010000209 DAVGJL010000000
VERSION     DAVGJL010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.212"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            2..730
                     /locus_tag="VVE42_002035"
     CDS             2..730
                     /locus_tag="VVE42_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU3074164.1"
                     /translation="MSTKYLFIYNEIREKILCNKYTMNEQLPDEMTLAKQFACSRMTI
                     KKALDLLVSEGLIFRKRGQGTFVLSRGSSKRKLIVPERDIRGLTKISEDAHSTIDSRI
                     IHFKLEFANEFLAEKLQVALQSPVYNIYRLRIIDGKPYVLEQTYMSTDVIPGITEDIL
                     QKSIYNYIEGKLGLHIASATKILRASSSSENEQHYLQLLPTEPVFEVEQVAYLDNGTP
                     FEYSISRHRYDLFEFNSFTLRHSS"
BASE COUNT          390 a          177 c          197 g          397 t
ORIGIN      
        1 aatgagtaca aaatatttat ttatttacaa tgagattcgt gaaaagattc tttgtaataa
       61 atataccatg aacgaacaat tgcctgatga aatgacatta gctaaacagt ttgcctgtag
      121 tcgaatgacg atcaaaaaag ctttagactt gttagtttct gagggcttaa tttttagaaa
      181 acgtgggcag ggaacctttg ttctctctcg tggcagctca aaaagaaaat taatcgttcc
      241 agaaagagat atccggggac tgacaaaaat atctgaagat gctcattcta caattgactc
      301 gaggattatt cacttcaaat tagaatttgc aaatgaattt ttagcagaaa aactacaggt
      361 cgctttgcag agtccagttt ataatattta ccgcctgcgt attattgacg gtaaacctta
      421 tgttctggaa caaacttata tgagtaccga tgttattcca ggtattactg aagatatttt
      481 acaaaaatcg atttacaatt acattgaagg aaagttagga ttgcatattg ccagtgctac
      541 aaaaatcttg cgagcttctt ctagttcaga aaatgagcaa cattacttgc agctccttcc
      601 aacggaaccg gtatttgaag tagaacaagt ggcttatttg gataacggaa ctccgtttga
      661 gtactcgatt agtcgtcatc gctatgattt atttgaattt aattctttta cattacgaca
      721 ttcctcctag gagaaaatgt gaaaatgaag ccaatctttt acagactcta gtttaagaaa
      781 aatttaaaac agggcaagaa ggtcccatct atgcttaaat ggtttctctt ttctaaataa
      841 gatggcttta aaagagtgat cgttttatcc atcatgttga aaaatatctt cgtatagctt
      901 atagagtagg tactgaaatt gttcacctga tctacttctt atagttattt agttttaaat
      961 agtgtttcaa atattcttac actgacgaga agtttttgag tcttttcttg taacacatat
     1021 agtatactga ggttagaata gtagactgtg acttctaaca aattgctaga aatgaatttc
     1081 aatctcccaa tttatttgtt caaatattgt tttaatatat tgaataaatt ctgaatcata
     1141 atcatttaaa aaaattttat t
//
LOCUS       DAVGJL010000210         1149 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.213, whole genome shotgun sequence.
ACCESSION   DAVGJL010000210 DAVGJL010000000
VERSION     DAVGJL010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.213"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..662
                     /locus_tag="VVE42_002036"
     CDS             <1..662
                     /locus_tag="VVE42_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU3074165.1"
                     /translation="DDFVFLLHENCCYLAVSIGFKKELIEKHLASININPESFYSTLL
                     QPNQIILTKALERVAMEILNCKMDAPAADFFFKAKANEWISIVIDTYLNRKKYKIEID
                     DDKALEDVARFLDDHFATNVNQGTLEKISKMSGTKLKNLFKEKYCQSITEYTQRKRMN
                     VAETLLLNTELPIKEIAESVGYSSASKFSIYYKRYKGKLPSEVRNLACKHHNFKCDYC
                     D"
BASE COUNT          421 a          149 c          188 g          391 t
ORIGIN      
        1 aagatgattt tgttttttta ttacacgaga attgttgtta tcttgctgtt tccattggtt
       61 ttaaaaaaga gctaatagaa aaacatttag catctattaa cattaatcca gaatcttttt
      121 attcaacctt gcttcaaccg aatcaaataa tacttacaaa ggccttagaa agagtggcaa
      181 tggaaatatt aaattgtaag atggacgctc ctgccgctga ttttttcttt aaagcaaagg
      241 caaatgaatg gataagtata gtaattgaca catacttaaa tagaaaaaaa tataaaattg
      301 aaattgatga tgataaagca cttgaagatg tagcaagatt cttagatgat cattttgcaa
      361 cgaatgtaaa tcaaggcacg cttgaaaaaa tatctaaaat gagtggtact aaattaaaaa
      421 atttatttaa ggaaaaatat tgtcaaagca ttacagagta cactcaaaga aaaagaatga
      481 atgtagctga aactcttctt ttaaacactg aactacctat aaaagagata gcagaatctg
      541 ttggatattc atctgccagc aaattttcta tctattataa aagatacaag ggaaaacttc
      601 caagtgaagt ccgtaattta gcttgcaaac atcacaattt taaatgcgat tattgtgatt
      661 aaagtaattt tttacaagcc atgtttttat atgaatttaa gtgtatatgt gcatatatag
      721 tctgaataaa tttgatttga aaaatggata aaaaaataaa ggcagtccga agaccgccaa
      781 tatttatctc tctgcacctt tgctatggtc tttcttattt gacttagatt tgtcatctgt
      841 cttaaagctt tttatttgcc ctaatatgga ctttttatcc ttgtcttgac tctttacttg
      901 ttcttttgct tttaagagct tagaagacaa aaagagatat tatattaaaa caatatttct
      961 ccggtactgg gactgggacg ggactaaaaa tctgaaaacg tcctataatt aggtggattt
     1021 agtgatatta taaaaaatag aatctagtat tttgaatggt tttggatata ttgtacgttt
     1081 ggaaaagaag aatgggagta attttaaact aacaaaaaat gctttgaaat cagtgtttca
     1141 gagcatttt
//
LOCUS       DAVGJL010000211         1123 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.214, whole genome shotgun sequence.
ACCESSION   DAVGJL010000211 DAVGJL010000000
VERSION     DAVGJL010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.214"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(217..411)
                     /locus_tag="VVE42_002037"
     CDS             complement(217..411)
                     /locus_tag="VVE42_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pncT"
                     /protein_id="HEU3074166.1"
                     /translation="MEKIDYITLNEVELETISGGDDCFIGDIGWGLLKSIGGMIKPAP
                     YVPPVCIPKSSWNPAPPVPC"
     gene            complement(729..932)
                     /locus_tag="VVE42_002038"
     CDS             complement(729..932)
                     /locus_tag="VVE42_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin class II family protein"
                     /protein_id="HEU3074167.1"
                     /translation="MNTKMMSQFSVMDNEMLDRIEGGIFGVDDALFWAGLGYVAGSIV
                     DTAIDDFTNQCRKNPHQWFCVRV"
BASE COUNT          372 a          218 c          145 g          388 t
ORIGIN      
        1 tttatttatt agacttgttc agaaattatt tgaagcagta ttagctagtt agaaagtgaa
       61 tttttaacgt tccaaccagc cattagtttc tagttttcga ctcttgcaat aagctaaaat
      121 aatcctgaac aacacctgta tattatagtt acgaagattc aacaaaatag cgaacaagtc
      181 gattattcat tttttatata agtttacaat aactttttaa catggaactg gaggtgcagg
      241 attccacgaa ctttttggaa tgcatactgg aggaacatat ggagcaggtt ttatcatccc
      301 tccgatactc ttcaaaagcc cccatccgat atctccaata aaacagtcat ctccaccact
      361 aatcgtctct aattctactt cattcaaagt aatataatca attttttcca ttgttataat
      421 tcattccttt cgttttactc aaggcacaac acagaatgaa aaagtgttgt actctttatt
      481 ttataatgtt tataataata gtgagaaaac ctatcactac tacaaatcac ggggaggtga
      541 ataagtgagt gatatagcca atatctcgca tttttatagg tgtcattctt tctatcaagc
      601 acaagaactg tgactaggag cacgtaaact tataacagaa taagcgactc gtttgcaaaa
      661 gaatagtttc tatcactgca taggtcatta agttatataa attcttatac aatacaacac
      721 tactatatct atacacgaac acagaaccac tgatgaggat ttttccggca ttgattcgta
      781 aaatcatcaa ttgctgtatc aacaatgcta cctgcaacat agcctaaccc tgcccaaaac
      841 aaagcatcgt caactccaaa tattcctccc tctatcctat caagcatttc attatccata
      901 acagaaaatt gtgacatcat ttttgtattc atgacaaata ctcctttttt atttttgtct
      961 tgttgtaact ttgataagtt tagtatatca tcgtttttta aaatttttca tccagatctt
     1021 gaattgtcat cgaaacgtct tgaattagct tttttatttc aagccacctc tatttcaagc
     1081 cacctctaaa tgtttaaaaa aaataatttc taatcacttt ttt
//
LOCUS       DAVGJL010000212         1117 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.215, whole genome shotgun sequence.
ACCESSION   DAVGJL010000212 DAVGJL010000000
VERSION     DAVGJL010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.215"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(281..1018)
                     /gene="erm(B)"
                     /locus_tag="VVE42_002039"
     CDS             complement(281..1018)
                     /gene="erm(B)"
                     /locus_tag="VVE42_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEU3074168.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          349 a          175 c          188 g          405 t
ORIGIN      
        1 aggaacggat tttggtactt gatttactta aaaaacattt agaaaaagca ggaaaatgaa
       61 aaggtaccat aaacggtcgt ttatggtacc attcaaattt atccttattg tacaaaataa
      121 cagcgaaatt tttaaatcta ttccttatcg atacaaattc cccgtaggcg ctagggacct
      181 ctttagctcc ttggaagctg tcagtagtat acctaataat ttatctacat tccctttagt
      241 aacgtgtaac tttccaaatt tacaaaagcg actcatagaa ttatttcctc ccgttaaata
      301 atagataact attaaaaata gacaatactt gctcataagt aacggtactt aaattgttta
      361 ctttggcgtg tttcattgct tgatgaaact gatttttagt aaacagttga cgatattctc
      421 gattgaccca ttttgaaaca aagtacgtat atagcttcca atatttatct ggaacatctg
      481 tggtatggcg ggtaagtttt attaagacac tgtttacttt tggtttagga tgaaagcatt
      541 ccgctggcag cttaagcaat tgctgaatcg agacttgagt gtgcaagagc aaccctagtg
      601 ttcggtgaat atccaaggta cgcttgtaga atccttcttc aacaatcaga tagatgtcag
      661 acgcatggct ttcaaaaacc acttttttaa taatttgtgt gcttaaatgg taaggaatat
      721 tcccaacaat tttatacctc tgtttgttag ggaattgaaa ctgtagaata tcttggtgaa
      781 ttaaagtgac acgaatgttc agttttaatt tttctgacga taagttgaat agatgactgt
      841 ctaattcaat agacgttacc tgtttactta ttttagccag tttcgtcgtt aaatgccctt
      901 tacctgttcc aatttcgtaa acggtatcgg tttcttttaa attcaattgt tttattattt
      961 ggttgagtac tttttcactc gttaaaaagt tttgagaata ttttatattt ttgttcatgt
     1021 aatcactcct gaagtgatta catctataaa taaatacaga agttaaacga tttgtttgta
     1081 attttagtta tctgtttaaa aagtcataag attagtc
//
LOCUS       DAVGJL010000213         1105 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.216, whole genome shotgun sequence.
ACCESSION   DAVGJL010000213 DAVGJL010000000
VERSION     DAVGJL010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.216"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            360..>1105
                     /locus_tag="VVE42_002040"
     CDS             360..>1105
                     /locus_tag="VVE42_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019213953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HEU3074169.1"
                     /translation="MADSFHFSVNIISRGKGKSAVASAAYISGEKIKNEWDGVTHDYT
                     RKEKILVKNIILPDHIPKEFNDRSTLWNKVEMAEKNSNAQLARQFIIGLPKELSLSEN
                     KNLVERYIKENLTSQGMIVDYAIHDESQDKNGNIHCHIMTIMRPINEKGEFLAKSKKE
                     YILDEKGEKVLNKNGKPKTRKVELTTWNDTGNVEQWRENFSDLCNKYLERAGAEKRVD
                     HRSFKRQNSDYLPTIHLGSAASAMERKGIET"
BASE COUNT          499 a          159 c          197 g          250 t
ORIGIN      
        1 aatagatgat aatgtagaaa aatcatctaa agatgaaata acataattgt cccttggtta
       61 caagggcgca attatacacc ctaaagggtg cttgcgtcct gcggagtcaa acccttgcag
      121 ggagcaacaa ccattcgctt tttaaaagtc aagggtaaat aaactcgcta aggctcgccc
      181 ttgactttta aaatttgaaa tgaaagtaac aattaagccg acatactgaa acaacaaaaa
      241 ttaattcagt agtcggcttt ttttattcta tcatttcaaa acgctcattc acaaagagga
      301 tataaaaaaa ctattaagcc tccacctaaa acaacaaaga aagaaaggaa gtgataaaaa
      361 tggcagacag ttttcatttt tcagtaaaca taatatcaag aggaaaaggc aaaagtgcag
      421 tagcaagtgc agcatatata agtggagaaa aaataaaaaa tgaatgggac ggagtaaccc
      481 atgactacac aagaaaagaa aaaatattag taaaaaatat aatattgcct gatcatatac
      541 caaaagaatt taatgatagg tctaccttat ggaataaagt agaaatggca gaaaaaaatt
      601 ctaatgcaca actagcaaga caattcataa taggactacc aaaagagtta tctttaagtg
      661 aaaataaaaa cctggttgaa agatatataa aagaaaattt aacatcacaa ggaatgatag
      721 tagattatgc aattcatgat gagagtcaag ataaaaatgg aaatatccat tgtcatataa
      781 tgaccataat gcgaccaata aacgaaaaag gagagttctt agccaagtca aaaaaagaat
      841 atatcctaga tgaaaaagga gaaaaagtat taaacaaaaa tggaaaacca aagacaagaa
      901 aagtagaact aacaacctgg aacgatacag gcaatgtaga acaatggaga gaaaattttt
      961 cagatctttg taacaaatat ttagaaagag caggagcaga aaaaagagta gaccatagga
     1021 gttttaaaag acaaaattca gattatctac cgacaatcca tttaggatca gcagcaagtg
     1081 caatggaaag aaagggaata gaaac
//
LOCUS       DAVGJL010000214         1069 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.217, whole genome shotgun sequence.
ACCESSION   DAVGJL010000214 DAVGJL010000000
VERSION     DAVGJL010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.217"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            5..712
                     /locus_tag="VVE42_002041"
     CDS             5..712
                     /locus_tag="VVE42_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3074170.1"
                     /translation="MYHQFLLGNNKTLQVLKNVSLSASRGEFISILGISGSGKSTLLK
                     CISSLLEPTSGEVILNGINPYKIRNAKLSSIRRNEVSFIFQAYNLIPSLPVIENIALP
                     LRLSQKKLTIKNVENLLKRMKFNAGLNDFVGTLSGGEQQKVAIARAVIADSDIIFADE
                     PTGALDSVSREVIFELLRELVGAGKCVIMVTHDIELASKTDRALILKDGKIFKELHRP
                     SGEELYKILEVQSTTEE"
     gene            715..>1069
                     /locus_tag="VVE42_002042"
     CDS             715..>1069
                     /locus_tag="VVE42_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3074171.1"
                     /translation="MLRLVYYQFLHNKKQWLGVSPVIFVSSLVMGLAVNGVINVENNS
                     QVFVGLPDPKPIFMFPIVFGGVTLFFVLSNIINMLVEIFRDDYELLEVLGASRLQLSF
                     LVGGQIFIISSIISFI"
BASE COUNT          347 a          142 c          223 g          357 t
ORIGIN      
        1 agggctgtat catcaatttt tactaggaaa taataaaacg ttgcaagtgc tgaaaaatgt
       61 ttctctttct gcttcgagag gagaatttat aagtattcta ggaattagtg gttctggaaa
      121 gtcaacttta ttaaaatgta tttctagttt gcttgaacca acaagtgggg aagtaatttt
      181 aaatggaatc aacccctata aaatcagaaa tgcaaaattg tcaagtataa gacgtaacga
      241 agtatctttt atatttcaag catacaattt aataccttcc ctgccggtaa tagaaaatat
      301 agcacttcct ttgcgattat cacaaaaaaa attaactatt aaaaatgtag aaaacttact
      361 caaaagaatg aagtttaatg ctggcttaaa cgattttgtt ggaactctgt ctggtggaga
      421 acaacagaag gttgctatag ctagagcggt tattgctgat agtgatataa tatttgctga
      481 tgagccaact ggggctttag acagcgtttc tcgtgaagta atttttgaat tattgagaga
      541 gttagtaggg gcgggtaagt gtgtaattat ggtaacgcac gatatagaat tggcctcgaa
      601 aactgatcgt gcattaatat tgaaagatgg aaaaattttc aaagaacttc atagacctag
      661 cggggaagag ttgtataaaa tcttagaggt acaatcaact acggaggaat agagatgctc
      721 agattagttt actatcaatt tttacataat aagaaacaat ggttaggcgt ttcaccagta
      781 atttttgttt cgagtttagt tatgggattg gctgttaatg gggtaattaa tgttgagaat
      841 aattcgcaag tatttgtcgg acttccagat cctaaaccaa tttttatgtt tccgatagta
      901 ttcggaggag taacattatt ctttgtgctc tctaacataa taaatatgtt ggttgaaatt
      961 tttagagatg attacgaact tctggaggtg ttgggagcaa gtcgccttca actatcattt
     1021 cttgtggggg gacagatttt tatcatttcc tcgattatta gttttattg
//
LOCUS       DAVGJL010000215         1048 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.219, whole genome shotgun sequence.
ACCESSION   DAVGJL010000215 DAVGJL010000000
VERSION     DAVGJL010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.219"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            177..731
                     /locus_tag="VVE42_002043"
     CDS             177..731
                     /locus_tag="VVE42_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3074172.1"
                     /translation="MFEYKLQLAGQTWGYLEFETNTEKYGKVLLIIKGKKRLTNQFPL
                     VQKNKSGYLFEYAQMNTLYLNQHASYKNDEDSHSFPIQMELVSDEMIQEIEQATKNSN
                     IEKFMILTYEADSENNIISVDVVMPDARTGQLHLIQDLSEYIQSSSYHFEEAKYQDIP
                     NFSELSETEDFEIISRIEKQEGQK"
     gene            746..>1048
                     /locus_tag="VVE42_002044"
     CDS             746..>1048
                     /locus_tag="VVE42_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU3074173.1"
                     /translation="MFNGRVLKELRLLNGLSRAELAQRINLTEQAIWQFESNETKPKL
                     STKMHLANQFHVDLTYFEQEEESIRFDSSVIAFRNADLATRKTIDIQTMYLHKVDSL"
BASE COUNT          390 a          142 c          200 g          316 t
ORIGIN      
        1 atgttgaatc aggatctctt tgattcgctt gaggcacaaa aaattgtaga tactttgatg
       61 aaagggcaaa aagattatgt agatgaacgt ctagaaaaag agagacaatg atagtgccga
      121 atggttatgc atggacgcga cctaatcata ttgatactgc atttgcatca gcagatttgt
      181 ttgagtataa attacaatta gcaggacaga cttggggata tttagaattt gaaacaaata
      241 cagaaaaata tgggaaagta ttgttaatta taaagggtaa gaagcgactt acgaaccaat
      301 ttcctttggt acaaaaaaat aagagtggct acttatttga atatgctcag atgaatacac
      361 tttatcttaa tcaacatgct tcctacaaaa atgatgaaga tagtcattcc tttccaattc
      421 agatggagtt agtttctgat gaaatgattc aagaaattga acaagctact aaaaattcga
      481 atatcgaaaa atttatgatt ttaacttatg aggcggactc agaaaacaat attatatctg
      541 tagatgttgt tatgcctgat gcacgaactg gtcagttaca cttgattcaa gatttgtctg
      601 agtatattca atcaagttcg taccatttcg aagaagctaa atatcaagat attcctaatt
      661 tttcagaatt atcggaaaca gaagattttg aaattatttc aagaatagaa aaacaagaag
      721 gtcaaaagta gtaaggagta tgtaaatgtt taatggtcgg gtattgaaag aattacggct
      781 gttaaatggt ttaagtagag cagaattagc tcagagaatt aatttaacgg aacaagccat
      841 ttggcagttt gagtccaacg aaacgaaacc taaattatca accaaaatgc atttggccaa
      901 ccaatttcat gttgatttaa cttattttga acaggaagaa gagagcattc gatttgattc
      961 ttctgtaatt gcctttagaa atgcagacct agcaacccgg aaaacaatag atattcaaac
     1021 tatgtattta cataaggtag atagtttg
//
LOCUS       DAVGJL010000216         1038 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.220, whole genome shotgun sequence.
ACCESSION   DAVGJL010000216 DAVGJL010000000
VERSION     DAVGJL010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.220"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            1..873
                     /locus_tag="VVE42_002045"
                     /pseudo
     CDS             1..873
                     /locus_tag="VVE42_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          323 a          182 c          214 g          319 t
ORIGIN      
        1 cgatttggcg aaatgtaaaa aaatatgagg agttcggact cgactctctc cttcaagaaa
       61 cacgtggtgg tcgtaaccat gcatatatga cagttgagga agagaaagcc tttcttgccc
      121 gccatttgaa ggctgcagag gcaggagaat ttgttacaat tgatgcctta tttcaggctt
      181 ataaaaagga gttaggtcgt tcctacacac gtgatgcctt ctatcaactg ttgaagcgcc
      241 atggttggcg aaatattatg ccacgtccag aacatcctaa gaaagcagac gctcaaacca
      301 ttgtcgtgtc taaaaataaa atctcaatta aagaagacaa gaaagcgctt taaaaccagt
      361 agacgttttc ataaggttcg cttgatgtac caagatgaag ctggtttcgg tagaatcagt
      421 aaactgggat cttgttggtc tccaatagga gtaggtccac atgtccatag tcactatata
      481 cgagaatttc gctattgtta tggagctgtt gatgcccata caggcgaatc atttttctta
      541 atagctggtg gatgtaatac tgagtggatg aacgcctttt tagaagagct ttcacaagct
      601 tatccatatg attatttttt actcgttatg gacaatgcta tatggcataa atcaagtgcc
      661 ttaaagattc cgactaatat tggctttgca tttattcctc catacacacc agaaatgaac
      721 cccattgaat aagtgtggaa agagattcgt aaacgtggat ttaagaataa agcctttcga
      781 actttggaag atgtcataca aggactggag aaggagatga taaagtccat cgttaatcgg
      841 agatggacta gaatgctttg tgaagacaga tgagtataag gttgctttat aattcatagg
      901 tttattgtag catattttgt ttcgctggaa gtctattata caccatttgt taagcgtttt
      961 ctacaattcg ctattttttg aatcaatttt caaaaatcat tatatttctc tcctaaccta
     1021 aaagtacttt cagaacat
//
LOCUS       DAVGJL010000217         1036 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.221, whole genome shotgun sequence.
ACCESSION   DAVGJL010000217 DAVGJL010000000
VERSION     DAVGJL010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.221"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..978
                     /locus_tag="VVE42_002046"
     CDS             <1..978
                     /locus_tag="VVE42_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU3074174.1"
                     /translation="FSACSSNSVKNEENTSKEHAPDKIVLDHAFGQTILDKKPERVAT
                     IAWGNHDVALALGIVPVGFSKANYGVSADKGVLPWTEEKIKELNGKANLFDDLDGLNF
                     EAISNSKPDVILAGYSGITKEDYDTLSKIAPVAAYKSKPWQTLWRDMIKIDSKALGME
                     KEGDELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAADTSKFWIYTSKDPRANY
                     LTDLGLVFPESLKEFESEDSFAKEISAEEANKINDADVIITYGDDKTLEALQKDPLLG
                     KINAIKNGAVAVIPDNTPLAASCTPTPLSINYTIEEYLNLLGNACKNAK"
BASE COUNT          407 a          149 c          175 g          305 t
ORIGIN      
        1 tttagcgctt gttcttctaa ttctgttaaa aatgaagaaa atacttctaa agagcatgcg
       61 cctgataaaa tagttttaga tcatgctttc ggtcaaacta tattagataa aaaacctgaa
      121 agagttgcaa ctattgcttg gggaaatcat gatgtagcat tagctttagg aatagttcct
      181 gttggatttt caaaagcaaa ttacggtgta agtgctgata aaggagtttt accatggaca
      241 gaagaaaaaa tcaaagaact aaatggtaaa gctaacctat ttgacgattt ggatggactt
      301 aactttgaag caatatcaaa ttctaaacca gatgttatct tagcaggtta ttctggtata
      361 actaaagaag attatgacac tctatcaaaa attgctcctg tagcagcata caaatctaaa
      421 ccttggcaaa ctttatggag agatatgatt aaaattgatt caaaagcctt aggtatggaa
      481 aaagaaggtg atgagttaat caaaaatact gaagctcgta tatccaaaga attagaaaaa
      541 catccagaaa tcaaaggaaa aatcaaagga aaaaaagtat tatttactat gattaatgct
      601 gcagatacat caaaattctg gatttatact agcaaagatc caagagcaaa ttatttaaca
      661 gatttaggtc tagttttccc tgaatcatta aaagaatttg agagtgaaga tagttttgca
      721 aaggaaattt ctgcagaaga agcaaataag ataaatgatg ctgatgtaat cataacttat
      781 ggtgatgata aaactcttga agctttacaa aaagatcctc ttttaggtaa aataaatgca
      841 attaaaaatg gtgccgttgc tgtaattcca gataatacac cgttagcagc ctcatgcact
      901 ccaacaccac tttcaataaa ctatactatt gaagaatatc taaatctttt aggaaatgca
      961 tgcaaaaatg cgaaataaaa aacaaataaa cctaggcata atttttgtaa tctgcctagg
     1021 tcttcttatt acaata
//
LOCUS       DAVGJL010000218         1019 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.222, whole genome shotgun sequence.
ACCESSION   DAVGJL010000218 DAVGJL010000000
VERSION     DAVGJL010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.222"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            24..518
                     /locus_tag="VVE42_002047"
     CDS             24..518
                     /locus_tag="VVE42_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU3074175.1"
                     /translation="MQELDLTQSINKKVKTYSLGTKQKLALLLTLVTEPDILILDEPT
                     NGLDIESSQIVLAVLKKLALHENVGILISSHKLEDIEEICERVLFLENGLLTFQKVGK
                     DSHNFLFEIAFSSATDRDIFITKQEFGDIVQEEGLRITMSGNIQSSELFKFFNENSIK
                     VVDF"
     gene            831..>1019
                     /locus_tag="VVE42_002048"
     CDS             831..>1019
                     /locus_tag="VVE42_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3074176.1"
                     /translation="MFYNQQKNRMIYVLSNFLYAISVSIIYALNGIVLLVIVSKLGIP
                     GDLGLDFIVAIVVNTILLV"
BASE COUNT          347 a          126 c          179 g          367 t
ORIGIN      
        1 atcaagaaag atttagaagt ttgatccaag agttagattt gactcagtct attaataaaa
       61 aagtaaagac ctattctttg ggtacaaaac aaaaattagc tttgcttcta actctcgtta
      121 cggaacctga tatattgatt ttagatgaac cgactaatgg tttagatatt gaatcatcac
      181 aaatagtttt agcggttcta aaaaaattag ctttacatga aaatgtggga attttaatat
      241 cgagtcataa attagaagac attgaagaaa tttgtgagag agttcttttc ttggagaacg
      301 ggcttttgac atttcaaaaa gtaggaaaag atagtcataa tttcttgttt gagatagctt
      361 tttcatcagc tacagataga gacattttca ttaccaaaca agaatttggg gatattgttc
      421 aggaagaggg actgagaatt actatgtctg ggaatattca aagtagtgag ctttttaaat
      481 tttttaacga aaactctatt aaagtagttg atttttaaac taaaaaagag acgcttaaag
      541 atatttatct aaatcgttca aaataaagaa aggttataat catgaaatta aataaattga
      601 attttcttaa ggaaaatata agaaatttat attcatcagg cgtaatatat ctcggtttgc
      661 ttatctcgtt tataccgccg atattggtta cattctttat tctaaagact taagggacat
      721 cgcttggtat taagcatatt tcaaactttt atgctatgct cggtatgtta atggctgtta
      781 tacatgctaa tcgagtcatt agtagagatt tttcccacaa tacggtaagt ttgttttata
      841 atcaacagaa gaatcggatg atttacgtct tgtctaattt tctatatgcc atctcagttt
      901 ccattattta tgctttgaat ggcattgtgt tactagtcat agtaagtaaa ttgggtattc
      961 caggtgattt aggattagat tttatagtag ctattgtagt caatacaatt ttgttagtc
//
LOCUS       DAVGJL010000219          993 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.223, whole genome shotgun sequence.
ACCESSION   DAVGJL010000219 DAVGJL010000000
VERSION     DAVGJL010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..993
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.223"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            329..568
                     /locus_tag="VVE42_002049"
     CDS             329..568
                     /locus_tag="VVE42_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3074177.1"
                     /translation="MKLLDCILDYQERFNGKTCQVSTNYKYLEIFKVNFCLTDLHHLF
                     DLYKITRDYASQTKPAIQAGVFILEDFRNILCTMM"
     gene            complement(583..>993)
                     /locus_tag="VVE42_002050"
     CDS             complement(583..>993)
                     /locus_tag="VVE42_002050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3074178.1"
                     /translation="LQKIDKDTTFALFDSLFKHSIIFLHSYKNISDERACFLISLNNK
                     KRKLKPADNLYSHFISFTSVLRTLAYFLALVKCRVTGLNTPLLSLRKPKTADLLFLSS
                     KNMTSNFIVFLDKSLATMQQLASLDIEDIADKLT"
BASE COUNT          340 a          138 c          185 g          330 t
ORIGIN      
        1 taaaagtaat atagtagatt gaatctggaa tagtccgtta tgactgttaa aatatttcta
       61 gaaattaatt tgactttcct aatcgatttg ttcacatctt gtttcaatct actatacttt
      121 ctcgttaaac cgttcggcaa ttcatggtga catggacaca tagtagtaag cacactatgt
      181 ggcttggcag agctaaaaac tgttcccttg cgataagcct aataagcaca acatagggag
      241 ttagagaagc cgactctaat caaccacttt gggcaatagt gagaactgcc ccgtgctttt
      301 ttattttgag aaaatatgga gtttgtcgtt gaaattactt gattgtattt tggactatca
      361 agaaagattc aatggaaaaa catgtcaagt atcaacgaat tataagtatt tagagatttt
      421 caaagtgaat ttttgtttga ctgatttaca tcatttattt gacttataca aaatcacacg
      481 agattatgct agtcaaacaa aacctgctat tcaagctggt gtttttattt tagaagattt
      541 tagaaatatc ctatgtacaa tgatgtaatc gaaaggatat ctttatgtga gtttatcggc
      601 gatatcttct atatctaagc tagcaagttg ttgcatcgta gctaaggatt tatctaaaaa
      661 tactatgaaa ttggacgtca tattttttga ggataaaaat aaaagatccg cagttttagg
      721 tttacgaaga gacaaaagcg gagtatttaa accagttact ctacatttta caagcgctaa
      781 gaaatatgct aaagttcgta aaacagatgt gaaagaaatg aaatggctat aaagattgtc
      841 cgcaggctta agcttgcgtt ttttgttgtt taacgaaatt aaaaaacaag ctctctcatc
      901 ggaaatgttt ttataggaat gaaggaagat gatgctatgt ttaaaaagag aatcgaaaag
      961 ggcaaaagta gtatctttat ctattttttg gag
//
LOCUS       DAVGJL010000220          988 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.224, whole genome shotgun sequence.
ACCESSION   DAVGJL010000220 DAVGJL010000000
VERSION     DAVGJL010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.224"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            632..>988
                     /locus_tag="VVE42_002051"
     CDS             632..>988
                     /locus_tag="VVE42_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEU3074179.1"
                     /translation="MNYEGSKQLTDARFKCLVGVQCTTFEEMLAVLKTAYQLKHAKGV
                     RKPKLSLEDLLMATLHYVREYRTYEEIAADFGIHESNLLRRSQWVEVTLVQSGVTISR
                     TPLSSEDTVMIIDATEV"
BASE COUNT          320 a          145 c          181 g          342 t
ORIGIN      
        1 tgttaagcta tatttatttg gttctttagt ttgattgttg ttgagtataa ctatttttgt
       61 atagatgaaa atcttgttat tgttcaagat gttttgatga ccattcatga tttgagtaat
      121 ccaaggtcaa aatgagtgct atactagcag tgtaaagatt cttgttcaac taagataaat
      181 cacacattta ggaggaaatt ttatgaagaa aaaaatactt atcattttca tcttgtatct
      241 aatcatgtcc atctttcttt atccgcttag ggaaagtatt tggtatcagc tattttatac
      301 catagcctat gtgattgcag ttatgatcta ttttgcttta actaaaaaga aaggagcaaa
      361 gaaatgaaaa cttttcttgc taaaaaacgg aacatcttcc tttcgtttgg gggttgaagt
      421 attataaaaa gtagaagtta atgaagtata aaagggtaag tgtttttgca atacttaccc
      481 ttttataata gattccctgc gaaacaaaat atggtatagt atagtagatt gaaactagaa
      541 tagtacgcct ctacttctaa aacattgtta gaaatcgatt tgactgtcct gattgattta
      601 tcctgttctt atttcatttt actatagttc tatgaattat gaaggaagta aacaactaac
      661 tgatgcacga tttaaatgtc ttgttggtgt tcaatgcacg acttttgaag agatgttagc
      721 tgtattaaaa acagcttatc aacttaaaca cgcaaaaggt gtacgaaaac ctaaattaag
      781 cctagaagac cttcttatgg ccactcttca ctatgtgcga gaatatcgaa cttatgaaga
      841 aattgcggct gattttggta tccacgaaag caacttactc cgtcggagcc aatgggttga
      901 agtaactctt gttcaaagtg gtgttacgat ttcaagaact cctctcagct ctgaggacac
      961 ggtaatgatt attgatgcga cggaagta
//
LOCUS       DAVGJL010000221          986 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.225, whole genome shotgun sequence.
ACCESSION   DAVGJL010000221 DAVGJL010000000
VERSION     DAVGJL010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.225"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(822..>986)
                     /locus_tag="VVE42_002052"
     CDS             complement(822..>986)
                     /locus_tag="VVE42_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HEU3074180.1"
                     /translation="LMHNLLSLSSAKEEYINRVLNVLDKEYGGILRYINTELGISSQE
                     IEELKDRYLF"
BASE COUNT          336 a          174 c          119 g          357 t
ORIGIN      
        1 aattggataa actatagatt tcagcaaacg aagttatccc aatataaaaa tactttaata
       61 actgaagata ttgacaggaa gatattaaat tcctcattac tatcctttac tatgcagtat
      121 aattttaata tcatttacct acttccaaga tgctgaaaaa tactaagtca cactattatg
      181 atgttattat ttccattttt attttaacag atgagtcaat tctttcaact accccctccc
      241 ttataactat attcatgaag tattcttgat aataaacctc atttaacttc tcaaagtagt
      301 cagcttctgc aactgctatt atctcataac agattgattt ggtttcaata aatggaagct
      361 agaagccttg tcagctaccc tgcacagttg gtgttttttc atttttctct tcaaaaaaat
      421 tggtcggaag ttataataaa aaggtgatgt gtttgtggtg agttgaatta agagacaaac
      481 tcactttgtc ttaactttta aattgcctaa caaaaatacg gagattattc tctaagcttt
      541 aactgcaaca ttatgtctct acaaccaacg ataagtccag aaatctaccc aaataaccaa
      601 acaacctaca tttgttgaac tttgatgaca agtaggaaat tttacatttg ataaacacta
      661 ttttgtacgc attttgcaca taatttaacg tgctttccat caatttagtt aaggacttac
      721 atagaaaaca atccgttttt gcttatgtta aattagtaga attagacaaa aacaaaaccg
      781 ccgttcgcac tgttatatat tcatttttag tacttactat tctaaaaaag atatctatct
      841 ttcaattcct caatttcttg actacttatt cctagttctg tattaatata tctaagtatt
      901 cctccatatt ctttatcaag gacattcaat actcgattaa tatattcttc ttttgctgaa
      961 ctgagtgaaa gtaaattatg catcaa
//
LOCUS       DAVGJL010000222          984 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.226, whole genome shotgun sequence.
ACCESSION   DAVGJL010000222 DAVGJL010000000
VERSION     DAVGJL010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..984
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.226"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(37..120)
                     /locus_tag="VVE42_002053"
     tRNA            complement(37..120)
                     /locus_tag="VVE42_002053"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(84..86),aa:Leu,seq:caa)
     gene            complement(130..201)
                     /locus_tag="VVE42_002054"
     tRNA            complement(130..201)
                     /locus_tag="VVE42_002054"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(167..169),aa:Gln,seq:ttg)
     gene            complement(212..284)
                     /locus_tag="VVE42_002055"
     tRNA            complement(212..284)
                     /locus_tag="VVE42_002055"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(249..251),aa:His,seq:gtg)
     gene            complement(299..369)
                     /locus_tag="VVE42_002056"
     tRNA            complement(299..369)
                     /locus_tag="VVE42_002056"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(335..337),aa:Trp,seq:cca)
     gene            complement(374..454)
                     /locus_tag="VVE42_002057"
     tRNA            complement(374..454)
                     /locus_tag="VVE42_002057"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(418..420),aa:Tyr,seq:gta)
     gene            complement(467..539)
                     /locus_tag="VVE42_002058"
     tRNA            complement(467..539)
                     /locus_tag="VVE42_002058"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(504..506),aa:Phe,seq:gaa)
     gene            complement(543..616)
                     /locus_tag="VVE42_002059"
     tRNA            complement(543..616)
                     /locus_tag="VVE42_002059"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(580..582),aa:Met,seq:cat)
     gene            complement(630..719)
                     /locus_tag="VVE42_002060"
     tRNA            complement(630..719)
                     /locus_tag="VVE42_002060"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(681..683),aa:Ser,seq:tga)
     gene            complement(802..875)
                     /locus_tag="VVE42_002061"
     tRNA            complement(802..875)
                     /locus_tag="VVE42_002061"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(839..841),aa:Ile,seq:gat)
     gene            complement(910..980)
                     /locus_tag="VVE42_002062"
     tRNA            complement(910..980)
                     /locus_tag="VVE42_002062"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(946..948),aa:Gly,seq:tcc)
BASE COUNT          276 a          272 c          228 g          208 t
ORIGIN      
        1 ggaacgaaga ataacaacgt tcctaacact atactatacc ggcggccggg gtcgaaccgg
       61 cacgtccttg cggacactgg attttgagtc cagcgcgtct gccaattccg ccacgccggc
      121 aaatagtaac tggggtagct ggattcgaac caacgcatga gggagtcaaa gtcccttgcc
      181 ttaccgcttg gctatacccc aatataaaat aggcgagtga tggggatcga acccacgcat
      241 gccagagcca caatctggtg tgttaaccac ttcaccacac ccgccataat tctattaaca
      301 cgggcagtag gaattgaacc cacactgaag gttttggaga ccttagttct acctttaaac
      361 tatgcccgta aaatggaagg ggagggattc gaacccccga acccgaagga gcggatttac
      421 agtccgccgc gtttagcctc ttcgctaccc ttccaaaata tataaatggc gcgagacgga
      481 atcgaaccgc cgacacatgg agcttcaatc cattgctcta ccaactgagc taccgagcct
      541 tattgcggga gcaggatttg aacctacgac cttcgggtta tgagcccgac gagctaccga
      601 gctgctccat cccgcgttaa taatataaaa ggaggatgtg ggattcgaac ccacgcacgc
      661 ttttacacgc ctgacggttt tcaagaccgt tcccttcagc cggacttggg taatcctcca
      721 aaatagttta tacgggaaca agcccgtgtc ctcttagtga acagataata ttccaatccc
      781 ctagtaatgg actaaacact atgggcacga gtggactcga accaccgacc tcacgcttat
      841 caggcgtgcg ctctaaccac ctgagctacg cgcccaagtc aaaaacttgg tacaaagaac
      901 aaagttcaaa gcgggtgacg agaatcgaac tcgcgacaac agcttggaag gctgtagttt
      961 taccactaaa ctacacccgc taaa
//
LOCUS       DAVGJL010000223          949 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.227, whole genome shotgun sequence.
ACCESSION   DAVGJL010000223 DAVGJL010000000
VERSION     DAVGJL010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..949
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.227"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            792..>949
                     /locus_tag="VVE42_002063"
     CDS             792..>949
                     /locus_tag="VVE42_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3074181.1"
                     /translation="MDNDWNGLADLIANLIAKYAGALDLDNLPDPTPAKNQEMKNSFD
                     MAKTQIET"
BASE COUNT          383 a          121 c          179 g          266 t
ORIGIN      
        1 caaaaccact atgtgacaat cttaaaatgg tatgaagaag ataaagacaa actaagacag
       61 aaaggtttaa ataaaaaaat gaattatgat gtaggagaat ctttatagat aaaaagaggt
      121 ggaaaggcac tattaaagac tttaagttta aaaaggtatc aaagtatgcc agagataaaa
      181 aaatataaat atgacacttt aaaggcgatt agaaaattaa agaaatgcta gaagactttt
      241 agatgacagt taccaagagg gtctaatttt cactgctact tatgcagtag gaattggacc
      301 tttttatata agcaaattct atttcaaaat tggttaccaa gaaagacttc ttttcactgt
      361 taagtataag agggtaattt atagaatttt gttacatcaa gataaatatt ttctgatggt
      421 caaattttaa aaagattaat aactctcgat aaattttgaa ccttaacaat tgaatagacc
      481 aagacaagac gttaattatt tttggagcgt aataacctat ccgtcaagat ctttcggctg
      541 aataattcgg caaaaaaagt tctgctaagc aaaaagaaag tgagcgataa gtaagagttt
      601 gaatataggg agggataccc tatccgctat gaagaaaatg aggataatga tacttctaag
      661 gttgaagccg gctcatccgg aatagaggcg gaggtgagat tcctatgttg ctaatccaaa
      721 gcacttgtca atgtcaaaaa acttaattaa acaaataatt attagatata aaactttaga
      781 ggaggtatat attggacaat gactggaatg gattggctga tttaatagca aatctgattg
      841 caaaatatgc tggtgcttta gatttagata atctacccga tccaacgcca gcaaaaaatc
      901 aagagatgaa aaatagtttt gacatggcaa aaacacaaat tgagacgga
//
LOCUS       DAVGJL010000224          931 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.228, whole genome shotgun sequence.
ACCESSION   DAVGJL010000224 DAVGJL010000000
VERSION     DAVGJL010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.228"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..511
                     /locus_tag="VVE42_002064"
     CDS             <1..511
                     /locus_tag="VVE42_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002402802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEU3074182.1"
                     /translation="SLQIDALTHYGIDKLFQEKVTGAKKDRPQLEEMINLLREGDSVV
                     IYKLDRISRSTKHLIELSELFEELSVNFISIQDNVDTSTSMGRFFFRVMASLAELERD
                     IIIERTNSGLKAARVRGKKGGRPSKGKLSIDLALKMYDSKEYSIRQILDASKLSKTTF
                     YRYLNKRNA"
     gene            515..>931
                     /locus_tag="VVE42_002065"
     CDS             515..>931
                     /locus_tag="VVE42_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /protein_id="HEU3074183.1"
                     /translation="MAMKRILTTSQREQLLSVDHLSEEDFKAYFSFSDYDLEVINQHR
                     GKVNKLGFAIQLCLARYPGCSLSNWPIKSTRLTSYVSRQLHLDAIDLNSYDHRNTRAN
                     HFNEILEVFNYHRFGSANTQKQLIEYLIELALENDDS"
BASE COUNT          320 a          151 c          167 g          293 t
ORIGIN      
        1 tagtttacaa attgatgctc ttactcatta tggaattgat aaattatttc aagaaaaagt
       61 aactggtgcg aaaaaagacc gaccgcaatt agaagaaatg atcaacctac tacgtgaagg
      121 agattctgtt gtcatttaca agttagatcg aatttcacga tcaactaaac atttgattga
      181 actttctgaa ttatttgaag aacttagtgt caattttata tctattcaag ataacgtaga
      241 tacttcaacg tctatgggaa gattcttttt ccgagttatg gctagtttag cagaactgga
      301 acgggatatt attattgaac gaactaactc tggtcttaag gcagccagag tccgaggaaa
      361 aaaagggggc cgtccaagta aaggtaagct atcaattgat ttagctttaa aaatgtatga
      421 cagcaaagag tattctattc gtcaaattct tgatgcctct aaattaagca aaacaacctt
      481 ttaccgttac ctcaataaaa ggaatgctta agatatggct atgaaaagaa ttttaactac
      541 ttcacagcgt gaacaacttc tttctgtaga ccacttatca gaagaggatt ttaaagcgta
      601 ttttagtttt tctgattatg atctggaggt tattaatcaa caccgtggaa aggtcaataa
      661 actaggattt gcgatacaac tttgtttggc ccggtatcct gggtgttctt taagtaattg
      721 gccgattaaa tcaaccagac taacttctta tgtgagtcga cagctccatc ttgatgcaat
      781 tgatttaaat tcatatgatc atagaaatac acgtgcaaat cacttcaacg agatcttaga
      841 agtattcaac tatcatcgat tcggtagtgc taatacacaa aaacagttaa tagaatattt
      901 aattgaacta gctttagaaa atgatgactc t
//
LOCUS       DAVGJL010000225          891 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.229, whole genome shotgun sequence.
ACCESSION   DAVGJL010000225 DAVGJL010000000
VERSION     DAVGJL010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..891
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.229"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..611
                     /gene="pfbA"
                     /locus_tag="VVE42_002066"
     CDS             <1..611
                     /gene="pfbA"
                     /locus_tag="VVE42_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HEU3074184.1"
                     /translation="NIELLRVSDNLVVSENSIFGGKEGIVIEDSKGKITVLNNQFYNL
                     SGKYISFIKSNANGKEPVIRDSDGNFNIVTENGLYKIVTNNLSDKNEKEKNKEEKQSN
                     SNNVIDSNQKNGEFNSSKDNRQMNDKIDNKQDNKTEEVNYKIVGDGRETENHINKSKE
                     IVDVKQKLPKTGSNKIMELFLTVTGIGLLLTLKGLKYYGKDK"
     gene            595..891
                     /locus_tag="VVE42_002067"
     CDS             595..891
                     /locus_tag="VVE42_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3074185.1"
                     /translation="MVKINKICSIQGSSVENEDIVGSQNQYFWIIGGATDLYNSKEEI
                     GYSVSEVVHILSESLSVNCKESKTLKQIFETALLEVKDEIGLNSYELTEYSKMK"
BASE COUNT          383 a           88 c          165 g          255 t
ORIGIN      
        1 caaatattga attattacga gttagtgata atttagtagt ctcagagaat agtatattcg
       61 ggggtaaaga aggaattgtt attgaggatt caaagggtaa aataaccgtt ttaaataacc
      121 aattttataa tttatccggt aagtatatat cattcatcaa atctaatgca aatgggaaag
      181 aacctgttat acgtgatagc gatggtaatt tcaatattgt aacggagaat gggctttaca
      241 aaattgtaac aaataattta agtgataaaa acgaaaaaga aaaaaacaaa gaggaaaaac
      301 aatctaattc aaataatgta attgatagta accagaagaa cggagagttt aactcaagta
      361 aagataatag acaaatgaat gacaagatcg acaataaaca agataataag acagaagaag
      421 taaactataa aatagttgga gatggcagag aaactgaaaa tcatattaat aaatctaaag
      481 aaatagtaga tgtaaaacaa aaattaccaa agactggttc gaacaagatt atggaactat
      541 tcttaacagt gacaggaatt ggtttacttt tgacactaaa agggttgaag tattatggta
      601 aagataaata aaatttgttc gatacaagga agttccgtag aaaatgagga tattgtaggt
      661 tcacaaaatc agtatttttg gattattggt ggagctacag atttatataa ttctaaagag
      721 gagataggtt attcagtctc agaagttgta catattttat cagaaagttt gtcggttaat
      781 tgtaaagaat caaaaactct taaacaaata tttgaaactg ctttactcga ggttaaagat
      841 gaaatcggtt taaactcata tgaattaaca gagtatagta aaatgaaata a
//
LOCUS       DAVGJL010000226          891 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.230, whole genome shotgun sequence.
ACCESSION   DAVGJL010000226 DAVGJL010000000
VERSION     DAVGJL010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..891
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.230"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(<1..860)
                     /locus_tag="VVE42_002068"
     CDS             complement(<1..860)
                     /locus_tag="VVE42_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000528564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU3074186.1"
                     /translation="MGNTNDDFIKSIIYKRIPRTIFAILAGSSLAISGVLMQSVTRNP
                     IADPGILGINTGASLSVVIGLSFLGISSSISHISFAIIGGLVSAIFVYAIAVSGKAGL
                     TPIKLALSGTCVSMALSSFVSFLILPNNNVLDKFRFWQIGSLGAATLSSISTLLPFII
                     LGHLIAIFISSDLNALAMGDEMAVGLGVNVNRIRSLAIIASVLLCSSITAIGGPIGFV
                     GLIVPHFCGLFISKDIRTMTISSSFIGAELLLICDIIGRMLGKPGEIEVGIITAIIGG
                     PVLIYVTMKN"
BASE COUNT          321 a          172 c          131 g          267 t
ORIGIN      
        1 ctatttttca tagttacata aataagtact ggacccccga ttattgcagt aattatccct
       61 acttcaattt cacctggttt acctaacata cggccgatta tatcacatat aagcaagagc
      121 tctgcaccta taaaagatga agaaatggtc attgtgcgta tatctttgct tataaataag
      181 ccacaaaagt gaggaactat aagacctacg aagccaatag gtccaccaat tgcagtaata
      241 cttgaacata aaagcacact tgcaattatt gcaagtgatc ttatcctatt aacattaact
      301 ccaagaccaa cagccatttc atcacccata gctaaagcgt ttaaatctga tgaaataaat
      361 atagctatca agtgacctaa aattataaaa ggtagtagtg tagatataga agataatgta
      421 gctgctccaa ggctacctat ttgccaaaat ctaaatttgt ctaagacgtt attattcggt
      481 aaaattaaaa aacttacaaa actgcttaaa gccatactaa cacaagttcc tgataaggca
      541 agttttatag gggtaaggcc tgcttttccg cttacagcaa tcgcgtatac aaaaattgca
      601 cttactaagc caccaatgat tgcaaaactt atatggctta tgcttgatga aattcctaaa
      661 aaagaaagac caattactac actaagactt gctcctgtgt ttataccgag tatacctgga
      721 tcagctattg ggtttctagt aactgattgc atcaatacac cgcttatggc aagactagaa
      781 cctgctaaaa ttgcaaaaat agttctaggt attcttttat atataattga tttaataaaa
      841 tcatcatttg tattacccat tccaaaagct gctaaaaaat ctctgatatt a
//
LOCUS       DAVGJL010000227          883 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.231, whole genome shotgun sequence.
ACCESSION   DAVGJL010000227 DAVGJL010000000
VERSION     DAVGJL010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..883
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.231"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            76..258
                     /locus_tag="VVE42_002069"
     CDS             76..258
                     /locus_tag="VVE42_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HEU3074187.1"
                     /translation="MANDNKSHYLIYRVLGISFEEGENIDLYQNKGRFLYKYAGSFLE
                     EAAVLSFNEKFGTENT"
     gene            268..633
                     /locus_tag="VVE42_002070"
     CDS             268..633
                     /locus_tag="VVE42_002070"
                     /inference="COORDINATES: protein motif:HMM:NF021048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HEU3074188.1"
                     /translation="MPNSENTKPKTFEIDCLVGEKHAYEIKWWDATTDGDHITKEHTR
                     IKVIHNKGYIPIRLMFYYPNRTQAIKIQQTLETLYNGIGGKYYYGDSAWEHLRAVTGI
                     DLLSILTDIANKKTGVKSK"
     gene            630..>883
                     /locus_tag="VVE42_002071"
     CDS             630..>883
                     /locus_tag="VVE42_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEU3074189.1"
                     /translation="MTVLKGDNLEILKTIESSSIDLIYMDPPFFTQKTQKLSNNKNIM
                     YSFEDTWTSIEDYKEFLSVRLEECKRVLKNSGSIFVHCDK"
BASE COUNT          366 a          111 c          146 g          260 t
ORIGIN      
        1 aaagaaaaaa tcgaaaaatt agctaagaaa tattctgata acttaaacat caaagttcaa
       61 gagagagttc gtgaaatggc aaatgataat aagagccatt atttgatata cagagtttta
      121 ggtatttcat ttgaagaagg agaaaatatc gatttgtatc aaaataaagg tcgtttttta
      181 tacaaatatg ctggttcatt tttagaagaa gctgcagtac tatcctttaa cgaaaaattt
      241 ggtacagaaa atacttaaaa agttaacatt cctaattctg aaaatacaaa acctaagact
      301 tttgaaattg attgtttagt cggagaaaaa cacgcatacg aaataaaatg gtgggatgca
      361 actacagatg gagaccatat aactaaagaa cacactagaa taaaagttat tcataacaaa
      421 ggatatatac caattcggtt aatgttctac tatccaaata gaactcaagc tataaaaatt
      481 cagcaaactt tagaaacatt gtataacggt attggaggga aatattatta tggagattct
      541 gcctgggaac atttaagagc agtgaccggt attgatttac ttagtattct aacagatatt
      601 gcaaataaaa aaacaggggt aaaatcaaaa tgacagtatt aaaaggagat aacttagaaa
      661 tattaaaaac tattgaatcc tcaagtattg atttaatcta tatggaccct cctttcttta
      721 cacagaaaac ccaaaaatta tctaataaca aaaatattat gtattcattc gaagatacgt
      781 ggacttcgat tgaggattac aaagaatttt tgtctgtaag attagaagaa tgcaaaagag
      841 tgctaaaaaa tagtggcagt attttcgttc attgtgataa aat
//
LOCUS       DAVGJL010000228          872 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.232, whole genome shotgun sequence.
ACCESSION   DAVGJL010000228 DAVGJL010000000
VERSION     DAVGJL010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..872
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.232"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            15..>872
                     /locus_tag="VVE42_002072"
     CDS             15..>872
                     /locus_tag="VVE42_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase"
                     /protein_id="HEU3074190.1"
                     /translation="MDRLQTMLNKIQVDTYHKNGWLFVKYSNNKLTQGWKLHVSSQLK
                     DACNIFYIVAQELEKERCNYKVLDCLDELKKLNSPREVSPTANKFITIYPSSRKQAKR
                     IILNLKEKLEKYKAPRILSDFQCGEYSPIHYRYGAFKAIKNYNFSENKIVYMMKNSRG
                     ELVEDLRLSYPYLPEGVKDLFTDMEKNKLFKPSPEIPENHPLNHYNIECILKKSNRGN
                     VYRAIEKSSNQKVIIKHARPYVYSWNDGFSAIDELKSEAANLKVFQDKSYTTDLIEEF
                     YVNEDYFVVQ"
BASE COUNT          348 a          106 c          152 g          266 t
ORIGIN      
        1 tatttattat atttatggat agattacaaa caatgctgaa taaaatacaa gttgatactt
       61 accataaaaa tggatggtta tttgtgaagt atagtaacaa taaacttaca cagggatgga
      121 aacttcatgt aagcagtcaa ctaaaagacg cttgtaatat tttttatatt gttgcacaag
      181 agctggaaaa ggaaagatgt aactacaaag ttctagattg tttggatgag ttaaaaaaat
      241 taaattctcc tagggaagtt tctcctactg caaataagtt tatcactata tatccgtcta
      301 gtaggaagca ggccaaacga ataatattaa atctaaaaga aaagttagaa aaatataaag
      361 ctccaaggat tttatcagat tttcaatgtg gtgagtattc tccaatacat tatagatatg
      421 gtgcgtttaa agcaattaaa aattacaatt tttctgaaaa taaaattgtc tacatgatga
      481 aaaatagtag aggagaatta gtagaagatc tgagattaag ttatccatat ttgccagaag
      541 gggttaagga tttatttact gatatggaaa aaaataaatt atttaaaccc tccccggaaa
      601 tcccagagaa tcacccttta aatcactata atatcgagtg tattctaaaa aaatccaata
      661 gaggaaatgt ttacagagct atagaaaaaa gtagtaatca gaaagtgata ataaaacacg
      721 cacggccata tgtttatagt tggaatgatg gtttctctgc aattgatgaa ttaaagagtg
      781 aagcagcaaa tttaaaagta tttcaggata agtcctatac aacagattta attgaagagt
      841 tttatgtcaa tgaagattac ttcgtagtac ag
//
LOCUS       DAVGJL010000229          854 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.233, whole genome shotgun sequence.
ACCESSION   DAVGJL010000229 DAVGJL010000000
VERSION     DAVGJL010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.233"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(<1..162)
                     /locus_tag="VVE42_002073"
     CDS             complement(<1..162)
                     /locus_tag="VVE42_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEU3074191.1"
                     /translation="MNQDRNKLLSKIAYLYYIENLNQSQIAAKLGIYRTSISRMLTEA
                     RNAGIVKIEI"
     gene            complement(175..>854)
                     /locus_tag="VVE42_002074"
     CDS             complement(175..>854)
                     /locus_tag="VVE42_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU3074192.1"
                     /translation="EKLNVSKVTIRKDLDKLESKGLLHREHGYAVLNSGDDLNVRLSI
                     NYEIKRKIVQEAVKLVSDNETIMIESGSTCALLAEEICKQKRNVTIVTNSFFIANFVR
                     AYDSCRIIVLGGEFQKDSQVTVGPLLKEMIQTFHVCQAFVGTDGYDKEMGFTGKDLMR
                     SEVVQYISAVSDKVIVLTDSSKFDKRGTVRRFALSQVYEVITDEKLSKQNIATLENAG
                     IMVKVVS"
BASE COUNT          261 a          177 c           91 g          325 t
ORIGIN      
        1 tatttcaatt ttaacaattc ctgcattcct tgcttctgtt aacattctac taatagaggt
       61 tctataaatt cctaattttg ctgctatttg tgactgattt aagttttcaa tataatacag
      121 ataagcaatt ttagaaagca gtttattcct atcttgattc atacacttaa cctcttacga
      181 aactacctta accattatcc cagcattttc taatgtagct atattttgtt tagaaagttt
      241 ttcgtctgtt attacttcat agacttgact taaagcaaat cttcttactg tacctctttt
      301 atcaaattta cttgagtcag ttaggacaat gactttatcc gacactgctg aaatatattg
      361 aactacctca ctgcgcatta aatcttttcc tgtaaagccc atctctttat cgtagccatc
      421 tgtcccaaca aaagcttgac acacatgaaa agtctgtatc atttctttta ataaaggtcc
      481 tacagtcacc tgtgaatctt tctgaaactc accaccaaga acaataatac gacatgaatc
      541 ataagctctc acaaaatttg ctataaaaaa tgaatttgtt acaatcgtaa catttctttt
      601 ttgcttgcaa atttcctcag caagtaaagc acaggtcgat ccagattcta tcattattgt
      661 ttcattatct gacaccaatt ttactgcttc ctgaacaatt tttctcttaa tttcataatt
      721 aattgacaaa cgtacattta agtcatctcc actatttaat acagcatatc catgctctct
      781 gtgtaataaa ccttttgact ctaatttatc taaatctttt ctaatcgtta ctttcgatac
      841 atttaatttt tccg
//
LOCUS       DAVGJL010000230          841 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.234, whole genome shotgun sequence.
ACCESSION   DAVGJL010000230 DAVGJL010000000
VERSION     DAVGJL010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.234"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(<1..786)
                     /locus_tag="VVE42_002075"
     CDS             complement(<1..786)
                     /locus_tag="VVE42_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016648332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3074193.1"
                     /translation="MALKSVITLTSGAIFYFVSGMFSHILGFRLETNLRKRGIDGLEK
                     ASFRFFDLNPSGQIRKIIDDNAAQTHQVVAHMIPDSSQAIITPVLVLALGFIVSIRVG
                     IILLALTIIGGLILGAMMGEQEFMKIYQESLSKLSAETVEYVRGMQVVKIFKANVESF
                     KSFYKAIKDYSKYAYDYSLSCKRPYVLYQWLFFGLIAILIIPIVYFMTSLASAKVILL
                     ELIMILFLSGVLFVSFMRMMWYSMYISQGNYAVDTLEALYEDMQ"
BASE COUNT          292 a          161 c          119 g          269 t
ORIGIN      
        1 ttgcatatct tcgtaaagcg cctctaaagt atctactgca taatttcctt gagaaatata
       61 catggagtac cacatcattc tcatgaatga aacaaagaga actcctgata aaaataaaat
      121 catgataagc tcaagtaaaa tcacctttgc gctagctaag ctagtcataa aataaactat
      181 aggaataatt aaaattgcaa tcagtccaaa aaataaccat tgatacaaaa cataaggcct
      241 tttacaagat agggaataat cataagcata ctttgagtaa tcttttatcg ccttataaaa
      301 gcttttaaaa gactctacat ttgctttaaa tatttttaca acttgcattc ctctcacgta
      361 ctcaacagtt tcagcactta gtttagatag ggattcttgg tatatcttca taaattcttg
      421 ctcgcccatc attgccccta aaattaagcc accaattata gtaagagcaa gcaaaattat
      481 gccaactctt atacttacta taaagccaag tgcaagtaca agtacgggtg tgattattgc
      541 ctgagaacta tcgggaatca tgtgtgctac cacctgatga gtttgtgcag cattgtcatc
      601 tataatcttt cttatttgac cagatggatt taagtcaaag aacctaaaac ttgctttttc
      661 cagaccatcg attccccttt ttcttaaatt tgtttcaagc ctgaatccca agatatgtga
      721 aaacattcct gagacaaaat aaaatatcgc tccacttgtt agtgtaataa cagattttaa
      781 tgctatgctc tctgcaccgg ataaatttga attaattatt aacttatcta gaaatttgta
      841 g
//
LOCUS       DAVGJL010000231          833 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.235, whole genome shotgun sequence.
ACCESSION   DAVGJL010000231 DAVGJL010000000
VERSION     DAVGJL010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.235"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..779
                     /locus_tag="VVE42_002076"
     CDS             <1..779
                     /locus_tag="VVE42_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU3074194.1"
                     /translation="VEYNLKKKFLTYFDFWTYSMGDAFICVSETMKKRLESIIKNKKI
                     VAINNGINVSNSDLDVVGVQDFKKEFCIPNNKKIICYVGRLDPEKRPDRFLEFAEKLF
                     LVREDVIFIMAGNGSMWAALKEKICHLKCRDNFRLLGEIYPATIVYQISDLLYIPSDT
                     EGIPMCVLESMSQGTPVLASNVGGLSEIIEHRVDGFLFEKEDVEGVCACANFLLNDSE
                     YLKYIGENSKSKIRKHFSVQKMFVETMRVYDELLEKSSHG"
     gene            772..>833
                     /locus_tag="VVE42_002077"
     CDS             772..>833
                     /locus_tag="VVE42_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU3074195.1"
                     /translation="MDRNIDQELVSIIIPTHNRY"
BASE COUNT          304 a           73 c          176 g          280 t
ORIGIN      
        1 gggttgaata taacttaaaa aagaaattct tgacttattt tgatttttgg acatattcta
       61 tgggagatgc ttttatttgt gtatctgaaa ctatgaaaaa aagattggag agtataataa
      121 aaaataagaa gatagttgca ataaataacg gaattaatgt atcaaatagc gatttagatg
      181 tagtaggtgt tcaagacttt aaaaaagaat tttgtattcc aaataacaaa aaaatcattt
      241 gttatgttgg aaggttggat ccagaaaaaa ggccggatag attccttgaa tttgcagaaa
      301 aattattttt agtcagagaa gatgtgattt ttattatggc tggaaatggt agtatgtggg
      361 ctgctttgaa agaaaaaatt tgccatttga aatgtagaga taatttcagg ttacttggtg
      421 aaatctatcc agctactata gtttaccaaa tatcggattt attgtatatt ccttctgata
      481 cagaaggaat acctatgtgt gttttagagt caatgtcaca aggaactccg gttttagcta
      541 gtaatgttgg cgggttaagt gaaattattg aacatagggt tgatggattt ttatttgaga
      601 aggaagatgt tgagggagtg tgtgcttgtg ctaatttttt actcaatgat tctgagtatt
      661 tgaaatatat aggtgagaat agtaaatcaa aaataagaaa acatttttct gtgcaaaaaa
      721 tgtttgtaga aaccatgaga gtatatgatg aattattaga gaagagtagt catggatagg
      781 aatatagatc aagaattagt aagtattata ataccaacac ataataggta tga
//
LOCUS       DAVGJL010000232          831 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.236, whole genome shotgun sequence.
ACCESSION   DAVGJL010000232 DAVGJL010000000
VERSION     DAVGJL010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.236"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..>831
                     /locus_tag="VVE42_002078"
     CDS             <1..>831
                     /locus_tag="VVE42_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEU3074196.1"
                     /translation="EKILSFDLIENTLKTEFERPIIKSNNTKYFSKVIWTKKDSYDVS
                     IPISLFWKSEDTDELPRRKKCILSVYGAYGKNDNSDLDEIMLSIIDAGFIYAIVHVRG
                     GGYLGGEWYRSGKALNKWNSIRDFIEGVNYLRENDVIDSKRLGLITSSAGGIIAGAVL
                     NEEKNLLQSILLFSPFINPYDTLQNPNDPLSKTEIAEWGDIRDPEVKAYIKSYSPMQN
                     IEKARDSNTVIVNILGEKDPYINNNEVIEWSKKLNSIGVKSLLYLNKAAGHGGFTPSD
                     V"
BASE COUNT          301 a           95 c          172 g          263 t
ORIGIN      
        1 cgaaaaaata cttagttttg atttaataga aaatacatta aaaactgaat ttgaaagacc
       61 catcataaaa tcgaataata ctaaatattt ttctaaggtg atttggacta aaaaagacag
      121 ttatgatgta agcataccga tttctttatt ttggaaaagt gaagatacgg atgagctccc
      181 aagaagaaaa aaatgtattc taagtgtcta tggggcttat ggtaagaatg ataattctga
      241 tttagatgaa attatgttat ctataataga tgcaggtttt atttatgcta tagttcatgt
      301 aagaggtgga ggctatttgg gtggtgaatg gtatcgctca ggaaaggcgt taaacaaatg
      361 gaattctatc agagatttta ttgagggagt aaattattta agggaaaatg atgtaattga
      421 cagtaagcga ttaggtttaa taacttctag tgcaggtgga ataattgctg gtgcggtgtt
      481 gaacgaggaa aaaaatttat tgcaaagcat tctcttattt tcgccattta taaatcctta
      541 tgatacactt cagaatccaa atgatcctct ttctaagacc gaaatagcag agtggggaga
      601 tattagagac cctgaagtaa aggcttatat aaagtcatat tcgcctatgc aaaatattga
      661 aaaggcacga gactcaaata ctgttatagt taatattttg ggtgagaaag acccatatat
      721 taataataac gaagtgatag agtggtcaaa gaaattaaat tctattggag ttaaaagttt
      781 gttgtattta aacaaagcag ctggtcatgg aggttttact ccatcagatg t
//
LOCUS       DAVGJL010000233          828 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.237, whole genome shotgun sequence.
ACCESSION   DAVGJL010000233 DAVGJL010000000
VERSION     DAVGJL010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.237"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            1..828
                     /locus_tag="VVE42_002079"
                     /pseudo
     CDS             1..828
                     /locus_tag="VVE42_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          250 a          179 c          145 g          254 t
ORIGIN      
        1 ctagaattct ccttagaaaa cgccgtttca agtgctatca ctgttcaaaa atgatggttg
       61 ctgagactcc cctggtaaag aaaaatcacc aaatccctcg tatcatcaac caaaaaattg
      121 ctcaaaagtt aattgaaaaa atttctatga ctgatattgc ccatcagctg gccatttcaa
      181 cttcaactgt cattcgcaag ctcaatgact ttcactttga gcatgatttt tctcggcttc
      241 caaagattat gtcttgggat gagtatgcct tcactaaggg aaagatgagt ttcattgcgc
      301 aagattttga taatcttaat attatcactg ttcttgaagg cagaacacaa gctgtcatcc
      361 gaaatcactt tcttcgctac gatagagccg ttcgttgtca agtgaaaatc attacgatgg
      421 atatgtttag tccttactat gacttggcta aacagcttcg ctttcaaatt tctaggctca
      481 ggctgaaaca gtctcccaga ctgtttcact cccgaatgct aaaatcgttc ttgatcgctt
      541 tcacattgta caacatctta gccgtgctat gagtcgtgtg cgtgttcaaa tcatgaatca
      601 gtttcatcga aaatcccatg aatacaaggc tatcaagcgc tactggaagc tcatacaaca
      661 ggatagtcgt aaactgagtg ataagcgatt ttatcgtcct acttttcgta tgcacttaac
      721 caataaagag attttaaaca agcttttgag ttattcggaa gacttgaaac accactatca
      781 gctctatcaa ctcttgctgt ttcactttca gaataaggaa ccggagaa
//
LOCUS       DAVGJL010000234          807 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.238, whole genome shotgun sequence.
ACCESSION   DAVGJL010000234 DAVGJL010000000
VERSION     DAVGJL010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..807
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.238"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
BASE COUNT          267 a          100 c          155 g          285 t
ORIGIN      
        1 aatagcaagt aatagaaata tggattaaat cttagaacgg cttaaaatca gacatgataa
       61 aggaaagcta tctaggtaac caagtactat aaacacagtc gaggatcact tgttaggatt
      121 acattttaat catgcttaat aagaatggag aattcctatg gggattgtat tactctgaag
      181 ttttgaatac cttagttctc gtgattttga gctattctag ctttagaatc cttcagaacg
      241 taaaatttta agcagtcaaa gacttagaat agcccgaaaa catttatttt ataacaattc
      301 taagattaga attgctcata tatatgtatt tgagctgctc gatagttagg acagctctat
      361 tctgagaaat ctgacctatc aatgcttcag aatagtattt cgcaaatggt ttttattgaa
      421 gaggtgattg ttttatgata gtctaaaata aaactgattt atggagtaaa tggagaatcg
      481 tcacatgaaa catttttttg caggaattgg tgagataaaa cttgttggct acttattgta
      541 tatatgtgta ggactagccc ctctttttca tatatatgtc ataggttcgg aactgagttt
      601 tgtgaaaata gtgttatcta ttttgggagt tatatttgta agtatgttaa ctattgctcg
      661 aatttatcgg acttttctat aaagaatagt ttggacttga actgtatcta aaaacagagg
      721 ttggtcgatt atcagcctct ttcttgtcgt tgaaaagagt gagatatatt gacttttgaa
      781 aaaaatgtca taattgagtt gggataa
//
LOCUS       DAVGJL010000235          797 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.239, whole genome shotgun sequence.
ACCESSION   DAVGJL010000235 DAVGJL010000000
VERSION     DAVGJL010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..797
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.239"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(<1..599)
                     /locus_tag="VVE42_002080"
     CDS             complement(<1..599)
                     /locus_tag="VVE42_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEU3074197.1"
                     /translation="MSNNTKYIFLSPHLDDAIFSCGDYISKLTSEGEIVLVITIFSGY
                     PLSQQLQPSAKQFHKLCNLGKYPIEERKKEDRLACERLQCDFRHLSYYECLYRKDRNG
                     NFLYRHIYSELKNEDTLKNDIIKELLMHLDDKCVVYCPLSLGDHVAHVFVNSIGRALE
                     FMRYKVIYYEDFPYVSDSSMVSYMGKTKELKMYQEELDE"
     gene            complement(611..>797)
                     /locus_tag="VVE42_002081"
     CDS             complement(611..>797)
                     /locus_tag="VVE42_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3074198.1"
                     /translation="FNKIVNGLIQNIEENLEDDIERILKYLYICLFNEIFIIKNKVNF
                     FDDVEFNQTLSEFLDKL"
BASE COUNT          275 a          156 c           87 g          279 t
ORIGIN      
        1 ttttcatcaa gttcctcttg atacattttt aattctttag tttttcccat ataactaacc
       61 atactactat cacttacata tgggaagtcc tcataatata ttactttata acgcataaat
      121 tcaagcgccc ttccaatact attcacaaaa acatgagcaa catggtcacc aagtgaaagc
      181 ggacaatata cgacacattt gtcgtctaaa tgcattaaca gctcttttat gatatcattc
      241 tttaatgtgt cctcattttt taattcacta tagatatgac ggtatagaaa attgccattt
      301 ctatctttcc tatagagaca ttcatagtac gataagtgtc taaaatcaca ttgtagacgt
      361 tcacaagcta acctgtcttc tttcttcctt tcttcaatcg gatatttccc aaggttacac
      421 aacttatgaa attgcttagc agagggctgt agctgttggc tcaaagggta accagaaaat
      481 atagtaataa caagtacaat ttctccttct gaagttaatt ttgaaatata atcaccacag
      541 gaaaaaattg cgtcatctaa atgtggagat aaaaagatat acttagtatt gttactcata
      601 accattccct ctacaattta tctaaaaact cactaagtgt ctgattaaat tccacatcat
      661 caaaaaaatt caccttattc ttaataatga atatttcgtt aaataaacat atatataaat
      721 atttcaatat cctttcaata tcatcctcta aattctcctc aatattttgt atcagcccat
      781 ttacaatctt attaaaa
//
LOCUS       DAVGJL010000236          762 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.240, whole genome shotgun sequence.
ACCESSION   DAVGJL010000236 DAVGJL010000000
VERSION     DAVGJL010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..762
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.240"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..598
                     /locus_tag="VVE42_002082"
     CDS             <1..598
                     /locus_tag="VVE42_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU3074199.1"
                     /translation="EYQIKNLSYKFTDDECLSLKDISFKLGKIYGIIGSNGRGKSTLL
                     RCLIGLEKKSKEEIYFKGEKLSKKERLKNSSLVMQDVNHQLFTDEVFNELRLGVKNFD
                     EEKAKIILKDLCLDEFIERHPMSLSGGQKQRLAIASVMCKNSPFVFFDEPSSGMDYSN
                     MIKISELINKYKTMDKIIFIVSHDIEFLNEVADEIFEL"
BASE COUNT          298 a           83 c          129 g          252 t
ORIGIN      
        1 agagtatcag ataaaaaatc ttagttacaa atttactgat gatgagtgtt taagcttaaa
       61 agatatttcg ttcaagcttg ggaaaattta tggcataata ggatccaacg gacgaggaaa
      121 atcaacgctt ttaagatgtt taataggtct tgagaaaaaa tcaaaagaag aaatttattt
      181 taagggagag aagctatcta aaaaagaaag actcaaaaac tcttcacttg ttatgcaaga
      241 tgtaaatcat caattattca cagatgaagt attcaacgag cttagattag gagtaaagaa
      301 ttttgatgaa gaaaaggcga aaatcatttt aaaagattta tgcctggacg aatttattga
      361 aaggcatccg atgagtttat caggagggca aaagcaaagg cttgcaatag catctgttat
      421 gtgcaagaat tctccatttg tcttttttga cgaaccttca agtggtatgg attattccaa
      481 tatgataaaa atatctgaac tgattaataa gtataaaacc atggataaaa taatttttat
      541 tgtttcccat gatatagaat ttttaaatga agtggcagat gaaatttttg aattgtaaaa
      601 tagagtctgt taaacatgtc tatagaacta gtaaattgtc taaaaattta aaagatacta
      661 acacatattg atatttatta gaaaattaac ttcaggattt ttacagtatt tataaacttt
      721 ataatatgta tttatagtat taataatttc catatcttta gg
//
LOCUS       DAVGJL010000237          758 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.241, whole genome shotgun sequence.
ACCESSION   DAVGJL010000237 DAVGJL010000000
VERSION     DAVGJL010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..758
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.241"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(198..335)
                     /locus_tag="VVE42_002083"
                     /pseudo
     CDS             complement(198..335)
                     /locus_tag="VVE42_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT          230 a          178 c          141 g          209 t
ORIGIN      
        1 agtcagtaac catacgcacg gcaaggcgac gttgacgtgg tttgaagaga ttttcgaaga
       61 gtattagaaa gctcgatgga ctattccaga ttcattccac tagatttcga aatcgagtag
      121 gggataatct ctagcccctc tcacaccacc gtacgtgccg ttcggcatac ggcggttcaa
      181 ctaactgtta acgcatgtca ttcaaggtaa taatccaaac acgaaaccag tccacgtttt
      241 tcaaggactg attttgatat agcacgttta agtaccgact tctgagatac taattgatag
      301 tggtcgcccc agccagatac cttatccgct atataggcct tattatcacg ttcctgttcg
      361 tagagccacg atttcgctat cccttcctct gacgattctt atttccagcg tcagacttaa
      421 aacggtctat ccccagaccg ttttaatccg ctacctcacg atagtcagac ttgggaatcg
      481 ctattgggtt caccggtagt aggtgcccac tgaggactta cacctcagat atacgacatg
      541 cccgtcgtac atcaaaaaaa gggctctata atatttgtag tgggtaaccc ccctatagat
      601 attatggagc ctattttgtg tagaaaaaaa gtcccatatg acctataatg aaaagcgaca
      661 aaacaactca ttagaaagat tcatatggaa caattacatt ttatcacaaa attactagac
      721 attaaagacc ctaatatcca gattttagac atcatcaa
//
LOCUS       DAVGJL010000238          730 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.242, whole genome shotgun sequence.
ACCESSION   DAVGJL010000238 DAVGJL010000000
VERSION     DAVGJL010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.242"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..231
                     /locus_tag="VVE42_002084"
     CDS             <1..231
                     /locus_tag="VVE42_002084"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3074200.1"
                     /translation="AKPTYPDKILVKNPDNIKDTEKNAIIEKLKEANKNHPAGAPTFA
                     KGEGEHANDIVATYSDGTTYYVPLNDVTKYAR"
     gene            374..544
                     /locus_tag="VVE42_002085"
                     /pseudo
     CDS             374..544
                     /locus_tag="VVE42_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT          263 a          115 c          151 g          201 t
ORIGIN      
        1 gctaaaccga catatccaga caaaatctta gtgaaaaatc ctgataatat aaaagataca
       61 gaaaaaaatg ccattattga aaaattgaaa gaggcaaata aaaatcatcc agcaggagct
      121 ccaacctttg ctaaaggtga aggagagcat gcaaatgata ttgtagcaac ttattcagat
      181 ggtacaactt attatgtacc gttaaatgat gtgacaaaat atgcgaggta gtggctgtac
      241 cactcactta ttcacctccc cgtgatttgt agtagtgata ggctttctca ctattattat
      301 aaaacaaaat aaagatcaca acactttttc attctgtgtt gtgccttgag tgaaacgaaa
      361 ggaatgaatt ataaatatga aaagtatagt cactagcata gatgagcgct tgcgtactcg
      421 cctacgagtg attatctgga agcaatggaa gaagaaatcg agacgattat ggggattgct
      481 taagttaggg gttcctaaat ggatagcaga taaggtatct ggctggggcg accactatca
      541 attatagtaa aatgaaataa gagtaggacg aattgatcag ggcagccaaa tcgatttcta
      601 acaatgtttt agaagaagag atgtactatt ctagtttcaa tctactatag tagctcagaa
      661 gtcggtactt aaacgtgcta tatcaaaacc agtccttgaa aaacgtgaac aggtttcgtg
      721 tttggattat
//
LOCUS       DAVGJL010000239          726 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.243, whole genome shotgun sequence.
ACCESSION   DAVGJL010000239 DAVGJL010000000
VERSION     DAVGJL010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.243"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            22..627
                     /locus_tag="VVE42_002086"
     CDS             22..627
                     /locus_tag="VVE42_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEU3074201.1"
                     /translation="MKNGKDVTYRGSANFIKDNVLITAAHNIYKHEFGKDAEELYVTP
                     ARTPNSVPFGRIKVKEVRYLKEFRNLNSKDAREYDLALLILEEPIGAKLGTLGLPTSQ
                     KNLTGITVTITGYPSYNFKIHQMYTDKKQVLSDDGMFLDYQVDTLEGSSGSTVYDASN
                     RVVGVHTLGDGANQINSAVKLNERNLPFIYSVLKGYSLEGW"
BASE COUNT          249 a           89 c          156 g          232 t
ORIGIN      
        1 gtagagtcag aatttcgtgg tgtgaaaaat ggtaaagatg taacttatcg agggagtgcc
       61 aattttataa aggataatgt ccttattact gctgctcata atatttataa acatgagttt
      121 ggtaaagatg cagaagaact atatgttact cctgctcgta ctccaaatag tgtgccgttt
      181 ggtcggataa aagtaaaaga agttcgttat ttgaaggaat ttagaaattt aaattctaag
      241 gatgcaaggg aatatgactt ggctttatta attctagaag agcccattgg tgcaaaatta
      301 gggactttgg gtcttcctac tagtcaaaaa aatttgacag gaataactgt gactatcaca
      361 ggctatccat catataattt taaaattcat caaatgtata cagataaaaa acaagtttta
      421 agtgatgatg gcatgttctt ggactaccaa gttgatactt tagaggggtc tagtggatct
      481 acagtttatg atgctagtaa ccgcgtagta ggagtgcata ctttaggaga tggagctaat
      541 caaattaaca gtgcagttaa attaaatgaa cgaaatttgc catttattta ttcggttctt
      601 aaaggttact ctcttgaagg atggtgaatg ggttaggtaa tgaaggctaa ttgtaaactg
      661 tgatggatac ttaactttgt ataataggtg gataaaagtc tttaaaatag gaaaaaacgc
      721 atatta
//
LOCUS       DAVGJL010000240          725 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.244, whole genome shotgun sequence.
ACCESSION   DAVGJL010000240 DAVGJL010000000
VERSION     DAVGJL010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.244"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(<1..>725)
                     /locus_tag="VVE42_002087"
     CDS             complement(<1..>725)
                     /locus_tag="VVE42_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3074202.1"
                     /translation="LPDIQFQTSAVGCIITVVLISFLAFLSGCFYTFKKIRNRKSSKI
                     RHVLSIVKRILLLAGFSVIWLLSLQQIFQDSTILAKAQIIFNIVILDIVIIYQLSPFI
                     QSCFIKLLSIIIFRNNFMFIVSKWNLLYCKSYIKSISAAITGAILLISSFQMISQNIL
                     SQFQDDSDLELKVAFIVYVGAPILIVLANIISIAFLSSHQERIEIQQFEILGTSNYQM
                     VKIKVGEAIFLTFVTSLIAFLLN"
BASE COUNT          288 a          113 c           95 g          229 t
ORIGIN      
        1 tgttaagtaa aaatgcaatt aatgaagtaa caaatgtcaa aaatatagcc tcaccaactt
       61 taatcttaac catctggtaa ttagaagttc ctaaaatttc aaattgctga atctcaatcc
      121 tttcttgatg cgatgacaaa aatgcaattg aaataatatt tgcaagtact atcaaaattg
      181 gtgctcctac atagacaata aatgctactt ttagctctaa atcactgtca tcttgaaatt
      241 gagatagtat attctgagaa atcatttgaa aactagaaat tagtaatata gctcctgtaa
      301 ttgcagcact gatagatttt atataagact tacaatatag taaattccac ttcgaaacaa
      361 tgaacataaa attatttcta aatataatta tagaaagtag tttgataaaa catgactgta
      421 taaaaggaga taattgataa ataatcacaa tatctaagat tacaatattg aatattatct
      481 gggccttcgc taaaattgtg ctatcttgga aaatttgttg caaagaaagc aaccagataa
      541 cactaaaacc agccaatagc agtattcttt ttactattga aagaacatgc cttattttag
      601 aactcttcct atttctaatc ttcttgaacg tataaaagca accacttaga aaggctaaaa
      661 atgaaatcaa cactactgta atgatacatc caacagcact cgtttgaaat tggatatcag
      721 gtaat
//
LOCUS       DAVGJL010000241          723 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.245, whole genome shotgun sequence.
ACCESSION   DAVGJL010000241 DAVGJL010000000
VERSION     DAVGJL010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.245"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..>723
                     /locus_tag="VVE42_002088"
     CDS             <1..>723
                     /locus_tag="VVE42_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HEU3074203.1"
                     /translation="AKNAYFYTPEEPSMIIREYYKKQGIFIISNEENLGQETSEKQNK
                     LYCRTKDFLEVLSENRSQDVNNADDLWNQLAFLDRLSFIDAKDFSRYSDLKKRALNWD
                     DEYCWFGNDQLRFEIDGHEELRIMVSKKSLLNRFLDMEIGEPRDLKGNRFLSKAFKLY
                     EEKQYSLAKAKFRELANIAFRQKDYFNFLVCEFNFQQIQIIDRYEKTPSYAEPLYDGE
                     LSELTEQILNSVTGDEKKIIEF"
BASE COUNT          270 a           81 c          139 g          233 t
ORIGIN      
        1 atgctaaaaa tgcttatttt tatactcccg aggaaccgtc tatgatcatt cgagagtatt
       61 ataagaagca aggaattttc attatctcta atgaagagaa tctaggtcag gagacatcag
      121 aaaaacagaa caaattgtat tgcaggacaa aagatttttt agaggttcta tcagaaaacc
      181 gaagtcaaga tgtaaataat gctgatgatt tatggaatca actggctttt ttagatagac
      241 ttagttttat tgatgcgaaa gatttttcta gatattctga tttaaagaaa agggctttaa
      301 actgggatga tgagtattgc tggtttggaa atgatcaatt aagatttgaa attgatggtc
      361 atgaagaatt acgaattatg gtatctaaaa aatctttatt aaatcgcttt ctagatatgg
      421 agattggtga gcctagggat ttaaagggga atcgattctt aagtaaggct tttaagttgt
      481 atgaagaaaa acaatattct ttagcaaaag caaaatttag agaactagct aatatagcat
      541 ttcgtcagaa agactatttt aatttcttag tttgtgaatt taattttcag caaattcaaa
      601 taattgatag atacgaaaaa acacctagtt atgcagagcc actttatgat ggagaattaa
      661 gcgaattaac agaacaaatt cttaactcgg ttacaggaga tgagaaaaag attatagaat
      721 ttt
//
LOCUS       DAVGJL010000242          723 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.246, whole genome shotgun sequence.
ACCESSION   DAVGJL010000242 DAVGJL010000000
VERSION     DAVGJL010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.246"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            282..500
                     /locus_tag="VVE42_002089"
     CDS             282..500
                     /locus_tag="VVE42_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001859328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEU3074204.1"
                     /translation="MKKISVTVIGYFEINIDENITDILYVNGTAILYPYLRSIVSIVS
                     AIDSSEAMLLPTINVLELLDKSQPFEEE"
BASE COUNT          235 a          100 c          132 g          256 t
ORIGIN      
        1 cgtcagttct atccacaacc tcaaaacagt gttttgagct gactacgtca gttctatcca
       61 taacttcaaa acagtgtttt gagcaacctg cggctagttt cctagtttgc tctttggttt
      121 tcattgagta taacacattg ttagaagttg gtttaaattt cctaatcagt ttgttcacat
      181 ttaccttcga tatattatat cccatagtta aggttggtca tacagatgat tatagtcatg
      241 gagccgtaaa acttagtgtt tctttagttg acaaagatgc catgaaaaaa atatctgtaa
      301 ctgtaatagg atattttgaa ataaatatag atgaaaatat caccgatatt ctatacgtaa
      361 atggtactgc tattctttat ccttatttac gttctattgt ttcaatagtt tcggcaattg
      421 atagcagtga agcaatgttg ctacctacca ttaatgtttt agagttacta gataaatctc
      481 aaccttttga agaagaataa tttattagct cactaaattg agggtaagga aaagtaaaag
      541 cagtaagaaa aatgtcttgc attatacagc aaccttttgg gaatgagtag atggattgaa
      601 taaaatttga ttaagagtgg atgatttatc tgtagattat tattggacag ttagtcttga
      661 agtagtctaa gaattaggtt ataatcagta gaagccttgc taataatgag gaggttagtt
      721 tat
//
LOCUS       DAVGJL010000243          716 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.247, whole genome shotgun sequence.
ACCESSION   DAVGJL010000243 DAVGJL010000000
VERSION     DAVGJL010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.247"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..133
                     /gene="blpO"
                     /locus_tag="VVE42_002090"
     CDS             <1..133
                     /gene="blpO"
                     /locus_tag="VVE42_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="HEU3074205.1"
                     /translation="SQFSVMDNEMLACVEGGDIDWGRKISCAAGVAYGAIDGCATTV"
     gene            237..356
                     /locus_tag="VVE42_002091"
     CDS             237..356
                     /locus_tag="VVE42_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEU3074206.1"
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKIFSKSSKKDTDSTL"
BASE COUNT          218 a           91 c          154 g          253 t
ORIGIN      
        1 gtcacaattt tctgttatgg ataatgaaat gcttgcttgc gttgaaggtg gagatattga
       61 ttggggaaga aaaattagtt gtgcagcagg ggttgcatat ggcgcaattg atgggtgtgc
      121 aacaacggtt tgacatttct attgggacca tttgctatag gaataggtgt aactggtgct
      181 gcaggtggag ctatacttgg aggtgtagcc tatgcagcaa catgttggtg gtaattatgg
      241 attttaaaag ttttattatt ggtttagtag ttggtatatt tggtccttat atggatgact
      301 taattagaaa aatattttca aaatcttcca aaaaggatac ggatagtact ctctaaaaaa
      361 taaatttctc ccctgtattt gctagcttaa tttacagggg agtttcttta atattgtttc
      421 gcctgtcaaa atgatatgtg atagcaggat gaaaagtcta caaacaagtt ggtatcaata
      481 caatataaag aggaaagtat gtttttcatg cttgcttttt taatttttac aattcaagag
      541 gttttgatga ccatttatga tttgagtgat ccaaggtcaa aataagtgct atactagcag
      601 tgtaaatgtt cttgctcaac taagataaat cgcacattta ggaggaaaac ttatgaagaa
      661 aaaaatattg attattttcg ttctgtatct gatcatgtcc atctttcttt atccgc
//
LOCUS       DAVGJL010000244          715 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.248, whole genome shotgun sequence.
ACCESSION   DAVGJL010000244 DAVGJL010000000
VERSION     DAVGJL010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..715
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.248"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(<1..660)
                     /locus_tag="VVE42_002092"
     CDS             complement(<1..660)
                     /locus_tag="VVE42_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001839792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3074207.1"
                     /translation="MVRDVIPINLVQEYCQGQPYGLLKKMLKRIRLSREISLLLATII
                     VYFFTHRILPLSVFTFIFSYILLFAQSYLGGNTAWIGNRRLIIDDEFEKILLSKSYIK
                     EISSARYSEYLTCEYKNFTPIILLAIFENLLDSYLLQNQSEVDLDIFYKVLPLLYKEK
                     YTMGFNYFVSLNYLLYKVGFLGIIYDNEALRDLSKQYLNKNISELQDGSFEGGIQDAV
                     AS"
BASE COUNT          256 a          117 c          103 g          239 t
ORIGIN      
        1 agaggcaaca gcatcttgta taccgccctc gaaagaacca tcttgtaatt ccgaaatatt
       61 tttgtttaaa tattgttttg acaaatctct taatgcttca ttatcgtaaa tgattcccaa
      121 aaatccaacc ttatatagta aataatttaa acttacaaag taattgaatc ccatagtata
      181 tttttcttta taaagcaaag gtaaaacttt ataaaaaata tctaagtcaa cctcactctg
      241 attctgaagc aagtaggagt caagcaaatt ttcaaaaata gctaacaata tgattggtgt
      301 aaagtttttg tattcacatg tcaagtattc tgagtatcgt gcagatgata tttcttttat
      361 ataacttttt gaaagcaaga ttttttcaaa ttcatcatct attattaatc ttctatttcc
      421 aatccaagcg gtattaccac ccaaataaga ttgtgcaaat agcaatatgt aagaaaatat
      481 gaacgtaaat actgaaagag gtagaattct atgcgtaaaa aaatagacga taatggtagc
      541 caggagtaaa gatatctctc gtgacaatcg aattcttttc agcatctttt ttaacagtcc
      601 gtaaggttgt ccttggcaat attcttgaac taaattaatc ggaatgacat ctctcaccat
      661 ttctggaaag tagagtaagt ttataggttg aaatctccag ctcccatcat aagta
//
LOCUS       DAVGJL010000245          698 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.249, whole genome shotgun sequence.
ACCESSION   DAVGJL010000245 DAVGJL010000000
VERSION     DAVGJL010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.249"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(56..226)
                     /locus_tag="VVE42_002093"
     CDS             complement(56..226)
                     /locus_tag="VVE42_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3074208.1"
                     /translation="MKKENEYVILTTASLGVMIGIVFAIFLDFPVEYGISLGLLNGIV
                     LGSLIVYKNNKN"
     gene            complement(239..685)
                     /locus_tag="VVE42_002094"
     CDS             complement(239..685)
                     /locus_tag="VVE42_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU3074209.1"
                     /translation="MNRSILDNITLKHEVTSQKIEEVCKAVQIYDEIMAMPMKFNTII
                     SEMGSNISGGQRQRIALARALINNPSIVILDEATSALDTINEERITKYIQSQGCTQII
                     IAHRLSTIKNADIIFVMKGGKIVESGNHKYLMTLGGEYYSLYTKRK"
BASE COUNT          219 a          147 c           76 g          256 t
ORIGIN      
        1 ttataatttc aactatagcc atctctactc cttagtttac agcaaaaaat tatgcttaat
       61 tcttattgtt tttgtaaaca atcagcgaac ccaatactat tccattcaac aagcctaaag
      121 aaataccata ttcaactgga aaatctaaaa aaattgcaaa cactattcca atcatcaccc
      181 ccagtgaggc tgttgttaaa attacatatt cattttcttt cttcattttc tttacacctc
      241 atttcctttt tgtatataag ctgtagtact ctccaccaag agtcattaag tacttatgat
      301 ttcctgactc aacaatctta ccacctttca ttacaaaaat aatatccgca ttcttaatcg
      361 ttgacaatct atgagctata attatttgag tacaaccctg actttgtata tactttgtta
      421 ttctttcctc attaatagtg tctaatgcac tagttgcttc atctaaaatt acaatactag
      481 gattatttat taatgcacgt gccagtgcta tccgttgcct ttgcccacct gaaatatttg
      541 accccatctc tgagatgata gtattaaatt tcatcggcat agccatgatt tcatcataga
      601 tttgaactgc tttacaaact tcctctatct tttgtgaagt aacttcgtgc tttaaagtta
      661 tattatcaag aatacttcgg ttcaataaaa atgaatcc
//
LOCUS       DAVGJL010000246          636 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.250, whole genome shotgun sequence.
ACCESSION   DAVGJL010000246 DAVGJL010000000
VERSION     DAVGJL010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.250"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..356
                     /locus_tag="VVE42_002095"
     CDS             <1..356
                     /locus_tag="VVE42_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3074210.1"
                     /translation="ESGHMKQALEEIFEYIRSANKFYDDMKPWALRESDIEKCKEVLA
                     TCVIIILNLGQMLNPFIPFSGKKIEDMFKTKLNTWNYISNLPNKLSDVSMLFDRIDLK
                     KIDEEVLELQQTSSR"
     gene            457..>636
                     /locus_tag="VVE42_002096"
     CDS             457..>636
                     /locus_tag="VVE42_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HEU3074211.1"
                     /translation="MNNLTLLKEYNFRDLGNHLTQTGQKIKPKTLFRSSKLFGISKID
                     VDLLQSYGITKVIDFR"
BASE COUNT          248 a           82 c          106 g          200 t
ORIGIN      
        1 ttgagagcgg gcatatgaaa caggcattag aagaaatttt tgaatatatt agatcggcta
       61 ataaattcta tgatgatatg aaaccgtggg cattgcgaga gtccgatatt gaaaaatgta
      121 aagaagtctt agctacttgt gtaatcatta tacttaattt aggacaaatg ttaaatccat
      181 tcattccttt ttctggtaaa aagattgagg atatgtttaa aacaaagcta aatacttgga
      241 attatatttc aaaccttcct aacaagctat cagatgtatc aatgttgttt gataggattg
      301 atttgaaaaa aattgacgag gaagttttag aattgcaaca aacatccagt agataattca
      361 aaaatactcg aaagtagcct tttttaattt aaattaacaa ccggagatgt tgaataaaat
      421 aaagatggtt ttttgagcat attatggaga aaagcattga ataatctaac tttactaaaa
      481 gaatataatt ttagagatct tggaaaccac ctcactcaaa caggacaaaa aataaaacct
      541 aaaacattat tccgttcaag taaactattt ggaatctcta aaattgatgt cgatttgtta
      601 cagagctatg gaataacaaa agtcattgac tttcga
//
LOCUS       DAVGJL010000247          625 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.251, whole genome shotgun sequence.
ACCESSION   DAVGJL010000247 DAVGJL010000000
VERSION     DAVGJL010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.251"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..>625
                     /locus_tag="VVE42_002097"
     CDS             <1..>625
                     /locus_tag="VVE42_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEU3074212.1"
                     /translation="LQLYEKKFLYTKKIKQLYCTFDNQFLPDRFVEGKCPNCGTHSRG
                     DQCDNCSAILDPIDLVDKRCSICSNEPEVRETEHFYYVFSEFQNLLETYLNDAEETVR
                     WRKNAINLTKRYLREGLPDRAVTRDLPNGIPVPIDGFRDKKIYVWFEAVAGYYTASVD
                     WAQKLQNNITDFWNNRTKSYYVHGKDNIPFHTIIWPAILSGLEIEPLP"
BASE COUNT          216 a           92 c          118 g          199 t
ORIGIN      
        1 ttttgcaatt atacgagaag aaatttttgt atacaaaaaa aattaaacaa ctctattgta
       61 catttgataa tcaattctta cctgataggt tcgttgaagg aaaatgcccg aattgtggaa
      121 cccattcaag aggagatcaa tgtgataatt gctcagcaat tttagatcct atagatttag
      181 ttgataaaag atgttctatt tgttccaatg aaccagaagt tcgtgagaca gaacactttt
      241 attatgtttt tagtgagttt caaaatttgt tagagactta cttaaatgat gctgaagaaa
      301 cagtgagatg gcgaaaaaat gcaataaatc taactaaaag gtatttacgg gaaggtttac
      361 ctgatcgagc tgttactcgt gatctaccaa atgggattcc agttcccatt gatggtttta
      421 gagataaaaa aatatatgtc tggtttgaag cggtagcagg ttactacaca gcaagtgttg
      481 attgggcgca aaaacttcaa aataatatta ctgatttttg gaacaatcgg acaaagtcat
      541 attatgttca tggaaaagat aacatcccat ttcatactat tatttggccg gcaatactct
      601 caggtttaga aatagagcct cttcc
//
LOCUS       DAVGJL010000248          615 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.252, whole genome shotgun sequence.
ACCESSION   DAVGJL010000248 DAVGJL010000000
VERSION     DAVGJL010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.252"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(1..615)
                     /locus_tag="VVE42_002098"
                     /pseudo
     CDS             complement(1..615)
                     /locus_tag="VVE42_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          198 a          105 c          132 g          180 t
ORIGIN      
        1 gactttcgat gaaactgatt catgatttgg acacgcacac gactcatagt acggctaaga
       61 tgttgtacaa tgtgaaagcg atccaacacg attttagcat tcgggagtga aacagtctgg
      121 gagactgttt cagcctgagc ctagaaattt gaaagcgaag ctgtttagct aagtcatagt
      181 aaggactaaa catatccata gtaataattt tgacgcgaca tcggacggct ctatcatatt
      241 taagaaagtg atctcggatg acagcttgtg ttctaccctc aagaacagtg atgatattga
      301 gattgttaaa atcttgcgca atgaagctca tctttccctt tgtaaaagca tactcatccc
      361 aagacataat ctcaggaaga caagaaaaat catgtttaaa gtgaaagtca ttgagcttac
      421 gaataacagt tgaagttgag atggaaagct gatgggcaat atcagtcata gaaatctttt
      481 caattaactt ttgagcaatc ttttggttga tgatacgagg gatttggtga ttcttcttga
      541 cgatagaagt ttcagcgacc atcatttttg aacagtgata gcacttgaat cgacgctttc
      601 taaggagaat tctag
//
LOCUS       DAVGJL010000249          608 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.253, whole genome shotgun sequence.
ACCESSION   DAVGJL010000249 DAVGJL010000000
VERSION     DAVGJL010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.253"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..81
                     /locus_tag="VVE42_002099"
     CDS             <1..81
                     /locus_tag="VVE42_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickase"
                     /protein_id="HEU3074213.1"
                     /translation="GKLKKLLSDKEKLSPKKWNEEKILLM"
     gene            407..580
                     /locus_tag="VVE42_002100"
     CDS             407..580
                     /locus_tag="VVE42_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019118552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3074214.1"
                     /translation="MNKRQELIDELIKANEDGTYKTYKSTEEIKAMNNEEIQIIYSNM
                     KNYLSDKRTHINY"
BASE COUNT          310 a           71 c           99 g          128 t
ORIGIN      
        1 gggaaattaa aaaaactcct atcagataag gaaaaactaa gtcctaaaaa gtggaatgaa
       61 gaaaaaattc ttttaatgta aaatcttgaa gaaataaata aagaaaaaga caagataaaa
      121 gatgaatacc aggaaattaa tcatataaaa tattcagtag attttgtaaa caaagaactc
      181 ggtatagatt tatccataga aatagataag ctaataaaac agggagaaaa accaagtgta
      241 atagcacaga ttaaaaagtt ccaagaccaa gtcaataaag ataatgaata cagagaaatg
      301 atgaaaaaca agaaaatgga tcaagaaaga taagacctgg taaaaatatc ttatccaagc
      361 tataatatga ccaaacaact aaagtcagat ctaggaggta atagagatga ataaaaggca
      421 agagctaata gatgaactca taaaagcaaa tgaagatgga acatacaaaa cctataaaag
      481 cacagaagaa ataaaagcaa tgaacaatga agaaatacag attatatatt ccaacatgaa
      541 aaactatcta tcagacaaaa gaacacacat aaactattaa aggtggaaag gtagtgtaag
      601 ctattttt
//
LOCUS       DAVGJL010000250          598 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.254, whole genome shotgun sequence.
ACCESSION   DAVGJL010000250 DAVGJL010000000
VERSION     DAVGJL010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.254"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..153
                     /locus_tag="VVE42_002101"
     CDS             <1..153
                     /locus_tag="VVE42_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU3074215.1"
                     /translation="KIVNGLQLPYSNAKLEATNNLIKLIKYNAFGFRNFENFKKERTK
                     FVLSQA"
     gene            complement(213..598)
                     /locus_tag="VVE42_002102"
                     /pseudo
     CDS             complement(213..598)
                     /locus_tag="VVE42_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          192 a          128 c          100 g          178 t
ORIGIN      
        1 aaaatcgtca acggccttca attaccttat tccaacgcca aactggaagc caccaataat
       61 ctcatcaaac ttatcaagta caatgccttt ggttttagga actttgaaaa cttcaaaaaa
      121 gaaaggacga aatttgtcct ttctcaagct tagcttttct tcaacccact acagttgaca
      181 aagagccgaa aaatgtctcg cctctaatat agtcaattga aacaagaaca agacaaaaga
      241 gcctcgtaaa aggtattgca acttggtaat acctttttga ggtgcttttt gatatgagcc
      301 catgttttct caataggatt gtactcaggt gagtagggag gaagaggtaa aagtttatgc
      361 ccaaactctt cgcataaaag ttctaacttc cccattctat ggaatcttgc attatccata
      421 ataataaccg atggtgtggt taatgttggt aagagaaact tctgaaacca agcttcaaaa
      481 aagtcgctcg tcatcgtctc ttcgtaagtc attggagcga ttaactcacc attcatttgt
      541 tagacctgca accaaagaaa tcctctgata tcttcttcca gatactttgc ctcttctt
//
LOCUS       DAVGJL010000251          590 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.255, whole genome shotgun sequence.
ACCESSION   DAVGJL010000251 DAVGJL010000000
VERSION     DAVGJL010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.255"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(7..486)
                     /locus_tag="VVE42_002103"
     CDS             complement(7..486)
                     /locus_tag="VVE42_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU3074216.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWDQEGMLCLHELISR
                     EEGLVDDIPRLRKYFKTKFRNRILDYIRKQESQKRRYDKEPYEEVGEISHRISEGGLW
                     LDDYYLFHETLRDYRNKQSKEKQEELERVLSNERFRGRQRVLRDLRIVFKEFTIRTH"
BASE COUNT          158 a          139 c           80 g          213 t
ORIGIN      
        1 gacttcctag tgggtacgga tagtaaactc cttaaacaca atgcgtaagt ctcttaatac
       61 tctttgacgc cctcgaaatc gttcattgct taagacgcgt tctagttctt cttgtttctc
      121 tttactttgt ttgtttctat aatctcttag tgtttcatga aagagataat aatcatctag
      181 ccagagacct ccctcactta tacgatgact gatctctccc acttcttcat agggttcttt
      241 atcgtatcta cgcttctgac tttcctgctt acggatatag tctaaaattc gatttcgaaa
      301 cttggttttg aaatatttcc ttaaacgtgg aatatcgtct accagtcctt cttctctact
      361 aatcaattca tgtaagcaga gcatgccttc ttggtcccaa tccgacaatt cccataaatg
      421 aaggtaatat tcatttctac acttataaac agtcccttgg acttcttcat acaattcttt
      481 aatcataatt ttcccctttc tagatacagt ctaacagatt agaaaacaca tttgttccat
      541 tttctccatt ctgttccctt tagctcctaa actgcacaaa aaagagaggc
//
LOCUS       DAVGJL010000252          585 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.256, whole genome shotgun sequence.
ACCESSION   DAVGJL010000252 DAVGJL010000000
VERSION     DAVGJL010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.256"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..585
                     /locus_tag="VVE42_002104"
     CDS             <1..585
                     /locus_tag="VVE42_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU3074217.1"
                     /translation="KLSIDFSFKSPFRVLLTGTSGSGKTTILNLINGSLKPQKGYVNL
                     LSHGKKSSDSIPTVDQTPYIFDTTIRENVTLFQNEYFSDDQIIEVLKKVNLYEELEKI
                     DILNYQCGENGSNLSGGQKQKIALARALIRNNKVYLFDEISANLDNDNSNSIHDILFN
                     LGISFIEVSHHYDLNDKRYTDIYKLENGTLFKIK"
BASE COUNT          230 a           66 c           93 g          196 t
ORIGIN      
        1 aaattaagta ttgacttctc atttaagtca ccatttaggg tattattaac aggaacttct
       61 ggtagtggga aaacaacgat tttaaattta attaatggtt ctttaaagcc acaaaaaggt
      121 tatgtaaatt tgttatcaca tgggaaaaag agttcagatt caataccaac agttgatcag
      181 acaccatata tttttgacac tactattcgt gagaacgtaa ctttatttca aaatgaatat
      241 ttttcagatg atcagataat tgaggtgtta aaaaaggtaa atctatatga agaattagaa
      301 aagatagata tactaaatta tcaatgtggt gaaaatggta gtaatttgtc tggaggtcaa
      361 aaacaaaaaa tagctttagc tagagctctg attagaaata ataaagtgta cttatttgac
      421 gaaatatcag ctaatttaga taatgataat tcaaattcca tacatgatat tctgttcaat
      481 ttaggtattt catttattga agtttcacat cattatgact taaatgacaa gagatacact
      541 gatatatata aattggaaaa tggaacactt ttcaaaatta agtga
//
LOCUS       DAVGJL010000253          581 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.257, whole genome shotgun sequence.
ACCESSION   DAVGJL010000253 DAVGJL010000000
VERSION     DAVGJL010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.257"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            158..442
                     /locus_tag="VVE42_002105"
                     /pseudo
     CDS             158..442
                     /locus_tag="VVE42_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996568.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
BASE COUNT          232 a           71 c          106 g          172 t
ORIGIN      
        1 tatcgcttac caatatttgc ttttatattt gctaaacttt cagaaaaaaa gttggagtat
       61 atgatgctag gtgactgcgt catgttagta aacgaaatgg aaataactga tcatagagta
      121 gataacttat ttgaaaaagg gaaaaatgag ataaaagatc catgtagtag atttagcaat
      181 aggacatata aaagctttag aaaaagtatc tgaaaaaaca gatgtttata tctataacct
      241 atgctcggga gaaggcacta gtgtattaca acttgtaaat acatttgaaa gtgttaataa
      301 gatccctatt ccttataaaa tagttccaag acgttcagga gacgttgcaa cttgttatgc
      361 aaatgcagac aaagcatata aggaattaaa ttggaggaca acaaaatcga ttgaagacat
      421 gtgtagagat acatggaatt agcaatcaaa aaatcccaat ggctataatt gatgatactg
      481 tctatatgag tgctgtattt tggaaaaatt acttaacctt ttaatgaaat gcatatttaa
      541 tgaaagataa attaaaaaaa atgtttgaca gcgcttgaca a
//
LOCUS       DAVGJL010000254          578 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.258, whole genome shotgun sequence.
ACCESSION   DAVGJL010000254 DAVGJL010000000
VERSION     DAVGJL010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.258"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(<1..>578)
                     /locus_tag="VVE42_002106"
     CDS             complement(<1..>578)
                     /locus_tag="VVE42_002106"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3074218.1"
                     /translation="LAEAEALVDADSEALVEADSDAEALVDADSDAEVLDEADALVLA
                     EAEALVLAEADALVDAEAEALVDAEADALVDAEADALVLAEADALVDAEAEALVDAEA
                     DALVLAEADALVDAEAEADVDADSDALVDAEAEALVLAEADALVDADSDAEVLAEAEA
                     LVDAEADALVDADSDAEVLAEAEALVDAEADAL"
BASE COUNT          138 a          197 c          130 g          113 t
ORIGIN      
        1 agtgcatcag cctccgcatc aacaagcgcc tcggcctcag caagcacctc agcatctgaa
       61 tcagcgtcaa ccagtgcgtc ggcttctgca tcaaccagcg cctcggcctc agcaagcacc
      121 tcagcatctg aatcagcgtc aaccagtgcg tcggcttcag caagcacaag tgcttcagct
      181 tcagcgtcaa caagtgcgtc tgaatccgca tcaacgtctg cctcagcctc agcgtcaacg
      241 agtgcgtcag cctcagcaag tacaagtgcg tcggcttcag cgtcaaccag tgcctcagct
      301 tccgcatcaa cgagtgcgtc agcctcagca agtactagtg catcagcttc cgcatcaacg
      361 agtgcgtcgg cttcagcgtc aaccagtgcc tcggcttccg catcaacgag tgcatcagct
      421 tcagcaagta ctagcgcctc agcttcagca agtacaagtg cgtcagcttc atcaagcacc
      481 tcagcatctg aatcagcgtc aaccagtgcc tcagcgtctg aatcagcctc aaccagtgcc
      541 tctgaatccg catcaacaag tgcctcggct tcagcaag
//
LOCUS       DAVGJL010000255          574 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.259, whole genome shotgun sequence.
ACCESSION   DAVGJL010000255 DAVGJL010000000
VERSION     DAVGJL010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.259"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..>574
                     /locus_tag="VVE42_002107"
     CDS             <1..>574
                     /locus_tag="VVE42_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU3074219.1"
                     /translation="IHQFGYTNHRIKLILSNEDLGATNARNIGIKNSRGKYISFLDDD
                     DEYMPDRILKLMACFKKSRMKNLALVYSYGIIIYPNGTREEEKTDFVGNPLFVQMVHN
                     IAGTSFWLCKKEVLELINGFEKIDSHQDGVVLLKLLAQGYQIDIVREFLVNYYAHSKE
                     NGITGVTQKTINADEEYYNYCRKYFNLLSF"
BASE COUNT          212 a           63 c          111 g          188 t
ORIGIN      
        1 ttatccatca atttggctat accaatcata gaattaagtt aattttaagt aatgaagatt
       61 taggtgcaac taatgccaga aacataggta tcaaaaattc tagaggtaag tatatatcat
      121 ttttagacga tgatgatgaa tatatgccag atcgaatttt aaagttgatg gcttgtttta
      181 aaaagagtag aatgaagaat ttagctttag tttatagtta tggaataata atttatccaa
      241 atggtacacg agaagaggag aagaccgatt ttgttggaaa tcccttgttt gttcaaatgg
      301 ttcacaatat agcaggtacg tcattttggt tgtgtaaaaa agaggtgcta gaattaatta
      361 atggttttga gaaaatagat tcacatcagg acggtgttgt tttattaaaa ctacttgctc
      421 aaggatacca aattgatata gtgcgagaat tcttggtgaa ttactacgct cacagtaaag
      481 aaaacggtat cactggagtg acacaaaaaa caattaatgc agatgaagaa tattataatt
      541 actgtaggaa atattttaat ttattgagtt tcaa
//
LOCUS       DAVGJL010000256          561 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.260, whole genome shotgun sequence.
ACCESSION   DAVGJL010000256 DAVGJL010000000
VERSION     DAVGJL010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.260"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            166..>561
                     /locus_tag="VVE42_002108"
     CDS             166..>561
                     /locus_tag="VVE42_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEU3074220.1"
                     /translation="MEDYCKEKNNISFKATFGDIKWSLLNKQAHRNTVSGDFGCLSNT
                     RKAQGRHLEQEGNIKHALIYYIESLIITSSGLENNFSATDYPVYYPDSIPDYSLKHIQ
                     TLMESLSDDDYDFAFDEALFRFSILNANHF"
BASE COUNT          221 a           80 c           79 g          181 t
ORIGIN      
        1 ttagctactt ctggaaaaaa agcagaatta attacaagaa taattaataa tgttaacatt
       61 gaaaatttag atattccgtt cgaatttaaa ctaacaaaag aagcacaaaa tcttattatc
      121 gaacatagtg actatatcaa agcatactat gataaagaca taactatgga agattattgt
      181 aaagaaaaaa acaatatctc ttttaaagca acttttggtg atataaaatg gagtctctta
      241 aataaacaag ctcataggaa tactgtatca ggagattttg gatgcttatc taacacacga
      301 aaggctcagg gaagacattt ggaacaagaa ggtaatatta aacatgcttt aatatattac
      361 atagaatctt tgataattac tagttcagga ttagaaaaca atttttcagc cactgattat
      421 ccagtatatt atcccgattc gatacctgac tactcactaa aacatattca aacattaatg
      481 gaatcattat ctgatgacga ttatgatttt gcttttgatg aagcattatt tcgcttctca
      541 attttgaatg caaatcattt t
//
LOCUS       DAVGJL010000257          560 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.261, whole genome shotgun sequence.
ACCESSION   DAVGJL010000257 DAVGJL010000000
VERSION     DAVGJL010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.261"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            79..>560
                     /locus_tag="VVE42_002109"
     CDS             79..>560
                     /locus_tag="VVE42_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RICH domain-containing protein"
                     /protein_id="HEU3074221.1"
                     /translation="MFKSNHERRMRYSIRKFSVGVASVAVASLFMGSVVHATEKEGST
                     QAATSFNRGNGSQAEQRGELDLERDKAMKAVSEYVGKMVRDAYVKSDRKRHKNTVALV
                     NQLGNIKNRYLNEIVHSTSKSQLQELMMKSQSEVDEAVSKFEKDSFSSSSSGSSTKPE
                     T"
BASE COUNT          211 a           81 c          123 g          145 t
ORIGIN      
        1 aataatgtag caaatttcat atacaattca tattagcagg atatactgta gttaattaaa
       61 attatggaga atatagacat gtttaaatca aatcatgagc gaagaatgcg ttattccatt
      121 cgtaaattta gtgtaggagt agctagcgta gctgttgcca gtctttttat gggaagtgtt
      181 gtacatgcga cagagaaaga gggaagtacc caagcagcca cttcttttaa taggggaaat
      241 ggaagtcagg cagaacaacg tggagaactc gatttagaac gagataaggc aatgaaagcg
      301 gtcagtgaat atgtaggaaa aatggtgaga gatgcctatg taaaatcaga tagaaaacga
      361 cataaaaata ctgtagctct agttaaccag ttgggaaaca ttaagaacag gtatttgaat
      421 gaaatagttc attcaacctc aaaaagccaa ctacaggaac tgatgatgaa gagtcaatca
      481 gaagtagatg aagctgtgtc taaatttgaa aaggactcat tttcttcgtc aagttcagga
      541 tcctccacta aaccagaaac
//
LOCUS       DAVGJL010000258          530 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.262, whole genome shotgun sequence.
ACCESSION   DAVGJL010000258 DAVGJL010000000
VERSION     DAVGJL010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.262"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            141..359
                     /locus_tag="VVE42_002110"
     CDS             141..359
                     /locus_tag="VVE42_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001207345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU3074222.1"
                     /translation="MALNYKPLWIQLAKKGLKKTDVIAMAGLTTNVMAQMGKDKPITF
                     KNLERICKALSCTPNDIISFEDNFSDEE"
BASE COUNT          232 a           61 c           93 g          144 t
ORIGIN      
        1 taaaacaatt tatggcaaaa taaggacgga ataacacaac aaattctcta aaacaaatca
       61 ctaaatcaat gtaagattga atgaaatcaa tatttatgct ataattaaat aaatctaatg
      121 aagaaaaaaa gagggatatt atggcactta actataaacc attatggata cagttagcaa
      181 aaaaaggact aaagaaaaca gatgtaatag ctatggcagg acttacaaca aatgttatgg
      241 cacaaatggg aaaggataaa ccaattacat ttaagaattt agaaagaata tgtaaggctt
      301 tatcttgcac tcctaatgat attattagtt ttgaagataa ttttagtgac gaggaataga
      361 aaatgacttt aaggacagaa gatcaagtta gggattatgc aagagaagta taggctttaa
      421 tgaagttgaa gaaaacatca atcaaggtac tggtcaaata actactttta atcaattagg
      481 cttcaaggga tattcagata agccagatgg ttggtattta cctaaaaata
//
LOCUS       DAVGJL010000259          526 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.263, whole genome shotgun sequence.
ACCESSION   DAVGJL010000259 DAVGJL010000000
VERSION     DAVGJL010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.263"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(<1..188)
                     /locus_tag="VVE42_002111"
     CDS             complement(<1..188)
                     /locus_tag="VVE42_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3074223.1"
                     /translation="MKNKRYFFDTILMILLLISTIFCVSPVFIKLDILGTPSHAILTF
                     VLAIPLFYILSQCLHTLL"
BASE COUNT          183 a          104 c           69 g          170 t
ORIGIN      
        1 aatagcaaag tatgtaagca ttgagacaaa atatagaata acggtatcgc caatacaaag
       61 gttaagatgg catgagaggg ggttcctaag atgtccaatt ttataaatac tgggctgaca
      121 caaaatatcg tgcttataag cagtaaaatc attaaaattg tgtcaaaaaa ataacgctta
      181 tttttcattt agtctacttt ccattgttaa ataatactac tgctaacacc aagccaactt
      241 agcacattgc atcacctaat cctgcgctac ttgtccataa tcctttaatg tttctactaa
      301 aattattcca atttccccca tcaagatagc ttatttatcc tagacaatgc ctactgcaac
      361 agtataaata agattcatca gccatctttt ctggtaatca tatgggtagc ctaatgatat
      421 ttctagttac tcaaaaatat ctagctatac tagagttatt tctaatccct aggacacttg
      481 ttaatccaaa tataacaaat tttcacccct taataggact ctttct
//
LOCUS       DAVGJL010000260          522 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.264, whole genome shotgun sequence.
ACCESSION   DAVGJL010000260 DAVGJL010000000
VERSION     DAVGJL010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.264"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(144..>522)
                     /locus_tag="VVE42_002112"
     CDS             complement(144..>522)
                     /locus_tag="VVE42_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEU3074224.1"
                     /translation="RNKNGQADTNQTEKPSEEKPQTEKPEEETPREEKPQSEKPESPK
                     PTEEPEESPEESEEPQVETEKVEEKLREAEDLLGKIQDPIIKSNAKETLTGLKNNLLF
                     GTQDNNTIMAEAEKLLALLKESK"
BASE COUNT           98 a          109 c           91 g          224 t
ORIGIN      
        1 agaggtatac tattctagtt tcaatctact atattttgca cattttcatg aaaaaaatga
       61 gaactagaac tcacattctg ctctcatttt tcgttttccc gttctcctat cctgttttta
      121 ggagttagaa aatgctgcta cctttactta ctctccttta ataaagccaa tagtttttca
      181 gcttctgcca taatagtatt gttgtcctgg gtgccaaata gtaaattatt ttttaatcct
      241 gtgagagtct ctttggcatt ggacttgata attggatcct ggatttttcc aagtaaatct
      301 tcagcctctc tcagtttttc ttcaaccttt tcagtctcga cctgaggttc ttctgattcc
      361 tctggtgatt cttctggttc ctctgttggt tttggagact ctggtttctc gctttgcggt
      421 ttctcttctc gaggggtttc ttcctcaggt ttttctgtct gaggtttctc ctcgcttggt
      481 ttttccgttt gattggtatc agcttgacca tttttgtttc tt
//
LOCUS       DAVGJL010000261          522 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.265, whole genome shotgun sequence.
ACCESSION   DAVGJL010000261 DAVGJL010000000
VERSION     DAVGJL010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.265"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(51..203)
                     /locus_tag="VVE42_002113"
     CDS             complement(51..203)
                     /locus_tag="VVE42_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3074225.1"
                     /translation="MTTNKDNDTSERSGCQRKRTAGEMPMNDLTSHTHGGNYTCYKEE
                     KVWNNQ"
     gene            complement(334..>522)
                     /locus_tag="VVE42_002114"
     CDS             complement(334..>522)
                     /locus_tag="VVE42_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU3074226.1"
                     /translation="KNLREDKDLSQCQIAQYLHCSQSAYSRIESGKQDIPTSFLKQLA
                     KLYQVTTDYILEMDKFSC"
BASE COUNT          154 a           98 c           83 g          187 t
ORIGIN      
        1 atttcttgag tataaaaagt taagcattga tagttgcata gtatacttta ttactgattg
       61 ttccaaactt tttcctcctt gtaacatgta taatttcctc cgtgggtatg actggttaaa
      121 tcattcatgg gcatttcacc cgctgtcctt tttcgctggc agcctgaacg ttcagaagta
      181 tcattatcct tatttgtagt catggcagat agagaaccac tctattttat agctaataat
      241 tatcgctcct atcacggcgt atcaagctaa agtgctcgac aaataagaaa gtcccacctc
      301 ggtttattca gttttcaaag ggcttaggat ttattaacaa ctaaacttat ccatctctaa
      361 aatataatct gtagttacct gatataattt agctaattgt ttcaaaaatg atgtaggaat
      421 atcttgcttg ccactttcta ttctagaata agcagactgt gaacaatgaa gatactgtgc
      481 aatttgacac tgggacaaat ctttgtcttc tcttaaattc tt
//
LOCUS       DAVGJL010000262          521 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.266, whole genome shotgun sequence.
ACCESSION   DAVGJL010000262 DAVGJL010000000
VERSION     DAVGJL010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.266"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..>521
                     /locus_tag="VVE42_002115"
     CDS             <1..>521
                     /locus_tag="VVE42_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HEU3074227.1"
                     /translation="YSFGKIKVTEEAKDYLLNRITYLQERLEITEDENLRELKNMLIF
                     LPLVDDIDIEKVIEILNNQTLYYNWSEEFRRIIKIVLDNMDVIDKDSLKSKIIGIVNK
                     HLNEILEKNFSLYNSVYPLYSQLLEYCSTDQETAIIVLEKFNTDILRIKYKNDDIKNI
                     IEYSDLICHLFKY"
BASE COUNT          211 a           56 c           77 g          177 t
ORIGIN      
        1 actattcatt tggtaaaata aaagttactg aggaagcgaa agactacttg ttgaatcgaa
       61 ttacttatct tcaagaaaga cttgaaatta ctgaagatga aaatcttcga gagcttaaaa
      121 atatgctaat ttttcttccc cttgttgatg atattgacat agaaaaagta atagaaattt
      181 taaataatca aacactgtat tataattgga gcgaggaatt taggagaatt attaaaattg
      241 tcctcgataa tatggatgtt attgataaag attcattgaa atctaagata attgggattg
      301 taaataaaca tttaaatgaa attttggaga aaaatttttc tttatataat tcagtgtatc
      361 cgctctattc acagttacta gaatactgct caactgatca ggaaactgca ataatagtac
      421 tcgaaaaatt taatacagat attttaagaa taaaatataa aaatgatgat ataaaaaata
      481 tcatagaata ttctgacttg atatgtcact tatttaagta t
//
LOCUS       DAVGJL010000263          512 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.267, whole genome shotgun sequence.
ACCESSION   DAVGJL010000263 DAVGJL010000000
VERSION     DAVGJL010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.267"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..237
                     /gene="blpM"
                     /locus_tag="VVE42_002116"
     CDS             <1..237
                     /gene="blpM"
                     /locus_tag="VVE42_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpM"
                     /protein_id="HEU3074228.1"
                     /translation="EQFHEMDITMLSSIEGGKNNWQTNVLEGGGAAFGGWGLGTAICA
                     ASGVGAPFMGACGYIGAKFGVALWAGVTGATGGF"
     gene            253..456
                     /gene="blpN"
                     /locus_tag="VVE42_002117"
     CDS             253..456
                     /gene="blpN"
                     /locus_tag="VVE42_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpN"
                     /protein_id="HEU3074229.1"
                     /translation="MNTYCNINETMLSEVYGGNSGGAAVVAALGCAAGGVKYGRLLGP
                     WGAAIGGIGGAVVCGYLAYTATS"
BASE COUNT          141 a           63 c          153 g          155 t
ORIGIN      
        1 gaacaatttc atgaaatgga tataactatg ttatctagta ttgaaggagg caagaataat
       61 tggcaaacta atgtcttaga aggtggtggt gctgcttttg gtggctgggg gttagggaca
      121 gctatttgtg ctgcgagtgg tgttggagcg ccatttatgg gagcatgtgg atacatagga
      181 gctaaatttg gtgtggctct ttgggcagga gtaacagggg caacgggtgg attttaacaa
      241 aaggagactt gtatgaatac ttattgtaat ataaatgaaa caatgctgag tgaggtttat
      301 ggaggtaatt ccggaggagc agctgtagtt gctgctttag gttgtgcagc gggtggagtg
      361 aaatatggga gacttctagg accatggggc gctgcaatag gaggaattgg aggagcagtg
      421 gtttgtggat atttagccta taccgctaca tcataaaaat gaaagctaaa ttttatgaag
      481 gctatattct ttattattct cttcgctttt ca
//
LOCUS       DAVGJL010000264          507 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.269, whole genome shotgun sequence.
ACCESSION   DAVGJL010000264 DAVGJL010000000
VERSION     DAVGJL010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.269"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            98..>507
                     /locus_tag="VVE42_002118"
     CDS             98..>507
                     /locus_tag="VVE42_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU3074230.1"
                     /translation="MSEYWFSTNVDQIDEVDGKQCLIYSYYNVKASRNVEVLKGRSGT
                     KKGLDYWEPYAPQKQYEMERLPKNKYIGSSSTDRWDGIEKNVVFCDCKEYVSAFDLFF
                     YHYNFKKISTQRSKQDFIRLRSKPVADILKNNTS"
BASE COUNT          190 a           59 c           84 g          174 t
ORIGIN      
        1 aaattttaat ttccctatta ctttattagt tggtagaaat ggagtaaata aaacttcaat
       61 tttacaagct ttatatggtt ctccagccaa taaaagtgtg tctgaatatt ggtttagtac
      121 aaatgttgat caaatagatg aagtggatgg gaaacaatgt ttaatctatt catattataa
      181 cgttaaagct tctagaaatg tagaagttct taaaggccgt tctggaacaa aaaaaggatt
      241 agattattgg gaaccatatg ctcctcaaaa acaatatgag atggaaagat tacccaaaaa
      301 taaatatatt ggaagttctt cgacagacag atgggatggc attgaaaaaa atgttgtctt
      361 ttgtgattgt aaagaatatg tttctgcttt tgatttattt ttctatcact ataattttaa
      421 aaaaatttct actcaacgta gtaaacaaga ttttataaga ttacgatcta aaccagttgc
      481 tgatatttta aagaataata caagtag
//
LOCUS       DAVGJL010000265          506 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.270, whole genome shotgun sequence.
ACCESSION   DAVGJL010000265 DAVGJL010000000
VERSION     DAVGJL010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.270"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..418
                     /locus_tag="VVE42_002119"
     CDS             <1..418
                     /locus_tag="VVE42_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEU3074231.1"
                     /translation="KNKADQDSKPDEDKEHDEVSEPTHPESDEKENHAGLNPSADNLY
                     KPSTDTEETEEEAEDTTDEAEIPQVEHSVINAKIADAEALLEKVTDPSIRQNAMETLT
                     GLKSSLLLGTKDNNTISAEVDSLLALLKKSQPVPIQ"
BASE COUNT          194 a           81 c          104 g          127 t
ORIGIN      
        1 taaaaataag gcagaccaag atagtaaacc tgatgaagat aaggaacatg atgaagtaag
       61 tgagccaact caccctgaat ctgatgaaaa agagaatcac gctggtttaa atccttcagc
      121 agataatctt tataaaccaa gcactgatac ggaagagaca gaggaagaag ctgaagatac
      181 tacagatgag gctgaaattc ctcaagtaga gcattctgtt attaacgcta agatagcaga
      241 tgcggaggcc ttgctagaaa aagtaacaga tcctagtatt agacaaaatg ctatggagac
      301 attgactggt ctaaaaagta gtcttcttct cggaacgaaa gataataaca ctatttcagc
      361 agaagtagat agtctcttgg ctttgttaaa aaaaagtcaa ccggttccta tacagtagta
      421 aaatgaatgg agcatatttt atggagaagt aacctttcgt gttacttctc ttttttagaa
      481 aaacgtaaca gaaacttgac aaaagt
//
LOCUS       DAVGJL010000266          504 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.271, whole genome shotgun sequence.
ACCESSION   DAVGJL010000266 DAVGJL010000000
VERSION     DAVGJL010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.271"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            3..>504
                     /locus_tag="VVE42_002120"
     CDS             3..>504
                     /locus_tag="VVE42_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEU3074232.1"
                     /translation="MGDESYSFSTLIKVLNSETVPGASFSIMEIRLPKLLAGIIAGWS
                     FGLAGFIFQTMLRNPLASPDIIGVTSSSSIAAVFCILVLKTNSLTTGIISITCGLTSS
                     LILFLLAKKDGFSAARLIILGIGFQTVTRAGTSFLLLKVARYELQEVMRWLSGSLSFT
                     KLDDIPL"
BASE COUNT          157 a           73 c           90 g          184 t
ORIGIN      
        1 ttatcggaga tgaaagttat tctttttcaa ctttgattaa agtcttaaat agtgaaactg
       61 ttcctggagc tagtttttcg attatggaaa ttagattacc aaaattatta gcaggaatta
      121 tagctggctg gtcttttgga ttggcaggat ttatctttca aactatgtta agaaatcctc
      181 ttgcaagtcc tgatataatc ggtgtcacaa gttcttcatc tattgcagcg gtcttttgca
      241 tattggtatt aaaaacaaat agtttaacta ctggaattat ttcaataact tgtggactaa
      301 catcatcttt aatattattt ttactagcta aaaaagatgg tttttcagca gcaagactga
      361 taatattagg cattggtttt caaactgtca caagagcagg cacctcattt ttattgttga
      421 aagtagcaag atatgaatta caagaagtta tgagatggct cagtggctct ttatctttta
      481 caaagttaga tgacatacct cttg
//
LOCUS       DAVGJL010000267          504 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.272, whole genome shotgun sequence.
ACCESSION   DAVGJL010000267 DAVGJL010000000
VERSION     DAVGJL010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.272"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(<1..486)
                     /locus_tag="VVE42_002121"
     CDS             complement(<1..486)
                     /locus_tag="VVE42_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU3074233.1"
                     /translation="MKIENESKSYDKCILKNISLEIDKGIYRVEGENGSGKSTLLQLL
                     AGLETFDSGLKNCVSKDVLYLDTNQLGVVPFTIEENLQLLCDSFQIHLSFEDKEQINT
                     FFDNKIGDNYMTASTGTKFKLGLSLIFAKNWDYIFIDETLSTVDARSIDMIAKRLISM
                     KE"
BASE COUNT          168 a           94 c           58 g          184 t
ORIGIN      
        1 ttctttcatg gaaattaagc gtttagcaat catatcaata cttcttgcat ccactgttga
       61 taaagtctca tcaataaaaa tataatccca atttttagca aaaattaagg ataagcctaa
      121 tttgaatttt gttccagtag atgctgtcat atagttatct ccgattttat tatcaaaaaa
      181 agtattaatt tgttctttat cctcaaatga tagatggatt tgaaaagaat cacacaacaa
      241 ctgcaaattt tcttctatag taaatggaac aaccccaagc tgattggtat caaggtatag
      301 tacatctttg gagacacaat tctttaaacc actatcaaaa gtctctagcc cagctaaaag
      361 ttgtagaagg gtgctctttc ctgaaccatt ctctccttca actctgtaaa ttcccttatc
      421 aatctctagt gatatatttt tcaatataca tttatcataa cttttagatt cattttcaat
      481 tttcataatt taattccctc tatc
//
LOCUS       DAVGJL010000268          490 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.273, whole genome shotgun sequence.
ACCESSION   DAVGJL010000268 DAVGJL010000000
VERSION     DAVGJL010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.273"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(<1..391)
                     /locus_tag="VVE42_002122"
     CDS             complement(<1..391)
                     /locus_tag="VVE42_002122"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3074234.1"
                     /translation="MRKSGFLIYYVLFCIVCFLLGLLLSFILKNETFATIPPKLELQF
                     TLTVLQANSTNFLLYLFCFPISPLLQLFDLLSLGIQLGISGQMIGIVATIKGILPHAL
                     IEIPNFIWYQGMSQYLLYRLLKDRGIKN"
BASE COUNT          205 a           85 c           56 g          144 t
ORIGIN      
        1 tatttttaat tcccctatcc ttcaataatc ggtataaaag atactgactc ataccttgat
       61 accaaataaa attcggtatt tcaataagag catgaggtaa tattcctttt attgtagcta
      121 ctattccaat catctgacca gatataccca attgtattcc taaagatagc aaatcaaaaa
      181 gttgcaataa gggcgaaatc ggaaaacaga atagatatag caaaaaattt gtagaatttg
      241 cctgtaaaac tgtcaatgta aattgtaatt ccagtttcgg aggtatagta gcaaaagtct
      301 cattttttaa tataaagctt aaaagtaaac ctagtaaaaa acagactata caaaataaca
      361 catagtaaat cagaaaacca ctttttctca tctaatgccc cctactaaaa ttacaaatat
      421 tatcagaaaa caatataaaa ccagaaaact ccatctcaat aaactaactg tacctttaat
      481 taaaaataga
//
LOCUS       DAVGJL010000269          476 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.274, whole genome shotgun sequence.
ACCESSION   DAVGJL010000269 DAVGJL010000000
VERSION     DAVGJL010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.274"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..440
                     /locus_tag="VVE42_002123"
     CDS             <1..440
                     /locus_tag="VVE42_002123"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEU3074235.1"
                     /translation="LMADGNAYSYAVREIVQYATDKKVDMIVGPEARGFIVGCPVAFE
                     LGIGFAPVRKPGKLPREVISADYEKEYGVDTLTMHADAIKPGQRVLIVDDLLATGGTV
                     KATIEMIEKLGGVMAGCAFLVELDELNGREKIGDYDYKVLMHY"
BASE COUNT          120 a           84 c          125 g          147 t
ORIGIN      
        1 ctttgatggc tgatggaaat gcttatagct acgctgttcg tgaaatcgtt cagtatgcta
       61 ctgacaagaa agtcgacatg atcgtgggac ctgaagctcg tggatttatc gtgggttgtc
      121 cagttgcctt tgagttggga attggttttg cgcctgttcg taagccaggt aaattgccac
      181 gcgaagttat ttctgctgac tatgaaaaag agtacggtgt cgataccttg actatgcacg
      241 cggatgccat taagccaggt caacgtgttc ttattgtaga tgaccttttg gcgacaggtg
      301 gaactgttaa ggcaactatc gagatgattg aaaaacttgg tggtgttatg gcaggttgtg
      361 ccttccttgt tgaattggat gaattgaacg gccgtgaaaa aattggtgac tacgactaca
      421 aagttcttat gcattattaa tgaaaacagt ccctagggct gttttctcta cactag
//
LOCUS       DAVGJL010000270          462 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.275, whole genome shotgun sequence.
ACCESSION   DAVGJL010000270 DAVGJL010000000
VERSION     DAVGJL010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.275"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
BASE COUNT          162 a           53 c           99 g          148 t
ORIGIN      
        1 ttagtgtaat tgagaaagga gaaatgattg tgattgatgt tggctaggtt atgttcaatg
       61 attcctaccg tctcaaatct tgtcagtaag gaaaaataaa ttcttcaaaa gtagagatta
      121 caaggcttgt ttaagaaaga attcaaagac cttgacaaat aaaaataaaa tggttattat
      181 aaaaaatggt ctgaaataga tgatgatact tttcgaaaat ctttttacgt cagctcagct
      241 ttgccttgct gtgttttgag caagctacgg ttagcttccg agtttgattt tcatttacta
      301 gaaatgaaac tgatgagaga tatcagtaga catctgagtc aggatattat ggaaaatgat
      361 aaaaagagct cgtgagattg gcatatcaga ctactaaagt attgagtttg ttaggatttt
      421 agcgactagt tagctgggaa aggaagatat ttgtgacaaa ta
//
LOCUS       DAVGJL010000271          458 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.276, whole genome shotgun sequence.
ACCESSION   DAVGJL010000271 DAVGJL010000000
VERSION     DAVGJL010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.276"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            104..>458
                     /locus_tag="VVE42_002124"
     CDS             104..>458
                     /locus_tag="VVE42_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA helicase"
                     /protein_id="HEU3074236.1"
                     /translation="MDNKRLIHAVAGSGKTTKIIESIDPQKRNLILTYTETNQNTIRA
                     KLIEKFGYIPESTFIFGVFEFLYSFCLVPYLGKRPKGINFDYKTQGKFDKTAIDNTGR
                     IVQNQLSKSLLRGSLI"
BASE COUNT          174 a           57 c           82 g          145 t
ORIGIN      
        1 tcagggaaaa ggctgaattt gcactgagat tactagaaca aatggaaact gataatataa
       61 gggaaaaatt tattataccc aattatatta aagaggcgat tgaatggata ataaaagatt
      121 gattcatgct gttgctggaa gtggcaaaac aactaaaata attgaaagta ttgatcctca
      181 aaaaagaaat cttatcctta cgtatacaga gacgaaccag aatacaatca gagcaaagtt
      241 gattgaaaaa tttggctaca ttcctgagtc tacttttatt tttggtgttt ttgagtttct
      301 ttatagtttt tgcttagtgc catatttagg gaaacgacca aaaggaataa attttgatta
      361 taaaactcaa ggaaaatttg ataagactgc tatagataat actggaagaa ttgttcagaa
      421 tcaactttct aaatctctat taaggggttc attaatct
//
LOCUS       DAVGJL010000272          441 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.277, whole genome shotgun sequence.
ACCESSION   DAVGJL010000272 DAVGJL010000000
VERSION     DAVGJL010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.277"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..>441
                     /locus_tag="VVE42_002125"
     CDS             <1..>441
                     /locus_tag="VVE42_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lanthionine synthetase"
                     /protein_id="HEU3074237.1"
                     /translation="NLQIENETLINYLLSLNMNRESRILNSNEFGEFVSPISFQHGLS
                     GYLFYLSHMRDISENGRIIEWIISEGNQNKGNVYLNGCSILFGSSGYLWVLLEFYKKI
                     TSNNPSRELIIDKIRDIYKDLISSYQKVEVFDFALGKSGILLSL"
BASE COUNT          159 a           47 c           77 g          158 t
ORIGIN      
        1 tcaatcttca aattgaaaat gaaacgctta ttaactatct tctttcgtta aatatgaata
       61 gagaaagcag gatactaaat tcaaatgaat ttggagaatt tgttagtcca atatcgtttc
      121 aacatggctt atcaggatat ttgttttatt tatcacatat gagagacatt tctgagaatg
      181 gtagaattat cgagtggatt atctctgaag gaaatcaaaa taaaggaaat gtgtatctaa
      241 atggttgttc aattttattt ggttcttccg gatatctttg ggtattatta gagttctata
      301 aaaaaattac cagcaacaat ccaagtagag aattgatcat agataaaatt agagatattt
      361 ataaagactt gataagtagt tatcaaaaag ttgaagtatt tgactttgct ttaggaaagt
      421 caggtatttt attgagtctt a
//
LOCUS       DAVGJL010000273          418 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.278, whole genome shotgun sequence.
ACCESSION   DAVGJL010000273 DAVGJL010000000
VERSION     DAVGJL010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.278"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            1..418
                     /locus_tag="VVE42_002126"
                     /pseudo
     CDS             1..418
                     /locus_tag="VVE42_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436304.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          129 a           83 c           73 g          133 t
ORIGIN      
        1 tcaaatgaag aaatatgact ttcaaaaacc ttctaaaatt ccttatcttg aaacgactgg
       61 tatgcctact agaattctcc ttagaaagcg tcatttcaag tgctatcact gttctaaaat
      121 gatggtcgct gagacttcta tcgtcaagaa gaatcatcaa attccttgta tcatcaacca
      181 aaagattgat cagaagctaa ttgaaaagac ttctatgact gatattgccc atcagctttc
      241 catctcaact tcaactgtca ttcgcaagat caatgatttt cactttgagc atgatttttc
      301 gcgtcttcct gagattatgt cctaggacgt tgaaacagtc tggggagtga ctgtttcaat
      361 cgggagatag agatgagctt tattgctcag gactttgata agctcaatat cataactg
//
LOCUS       DAVGJL010000274          412 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.279, whole genome shotgun sequence.
ACCESSION   DAVGJL010000274 DAVGJL010000000
VERSION     DAVGJL010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.279"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            32..>412
                     /locus_tag="VVE42_002127"
     CDS             32..>412
                     /locus_tag="VVE42_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU3074238.1"
                     /translation="MPKRYLLDGKTPRISATNIDNGILGYYEDIDDKNYRVFENFISV
                     SFLGAVFYHKYKASLDMKIHCLKLKNKELNKEVAFYLTSIIRQALKNTEYKDQISSTV
                     LPDIKIKLPIDSRGTPDWNYMERYI"
BASE COUNT          173 a           51 c           60 g          128 t
ORIGIN      
        1 aaaatcgagt caagtaccaa caggctcatc tattccaaaa agatatttac tcgatggaaa
       61 aactcctaga attagtgcta caaatattga caatggtatc ttaggttact acgaagatat
      121 agatgataaa aattatagag tttttgaaaa ttttatatct gtttcatttt taggagcagt
      181 cttttatcat aaatataaag caagcctaga tatgaaaatt cattgtttaa aattaaaaaa
      241 taaagaattg aataaagaag tagcattcta tttaacttca attattaggc aagctttaaa
      301 aaatactgaa tataaagatc aaatatctag cacagtattg ccagatatca aaattaaatt
      361 accgatagac agtagaggta ctccagattg gaattacatg gaaagatata ta
//
LOCUS       DAVGJL010000275          411 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.280, whole genome shotgun sequence.
ACCESSION   DAVGJL010000275 DAVGJL010000000
VERSION     DAVGJL010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.280"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
BASE COUNT          160 a           81 c           43 g          127 t
ORIGIN      
        1 cttacaaaaa tacccttgga atcaatcaat ggaaaataaa atattgcaac tagaagcgaa
       61 taaagtcccg ctaataaata tttctgatca tctttcattc aaaacaccat attatttact
      121 aagaatacat caatacaata gagcaactct gtaagtcaat atttgataaa gcaaagcttt
      181 aaccccttcc ccagacgtta gcttttcttc aacccactac agttgacaag gagcccaaat
      241 tattgataaa aactttagtc atcatcgtct ctataaaaat attttctata cactcttgat
      301 aataccaaaa ttaatgccaa tatcatacca agagctataa taatagaatt tattagattt
      361 attttagtat atccaatcac tagtccaata attggactag cagacatgct t
//
LOCUS       DAVGJL010000276          410 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.281, whole genome shotgun sequence.
ACCESSION   DAVGJL010000276 DAVGJL010000000
VERSION     DAVGJL010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.281"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
BASE COUNT          147 a           46 c           80 g          137 t
ORIGIN      
        1 aaaaattttt aaagtcttcg gagaagaaaa cagaaaaatc tgttaaaaaa taatcaaaac
       61 tataaatgat gaatctgaat caaaattatt ttgcgcatgt aaataggagt cttatagtaa
      121 cgagtcaaaa aaggagtaac tatgaatcgt aatttagaac ggtgttatct attctgacta
      181 ggaatagatc ataccagagg tggcttagaa atagcgggga cattagaaat tgaaggaact
      241 ttaaatagga tgtcgtaaat gttgctatca atgatttatt tgtcccaagc ttgcctaggg
      301 tgtcagtaaa aaatcatttt cctttcatat catattttta gtaggtagga tgtttgttct
      361 gtctattttt tgccaaaaaa agtgcagttg aagtgtttat ttggagtttt
//
LOCUS       DAVGJL010000277          407 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.282, whole genome shotgun sequence.
ACCESSION   DAVGJL010000277 DAVGJL010000000
VERSION     DAVGJL010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.282"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..>407
                     /locus_tag="VVE42_002128"
     CDS             <1..>407
                     /locus_tag="VVE42_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lanthionine synthetase"
                     /protein_id="HEU3074239.1"
                     /translation="GYLAVDISPTNFILDDFGRVRLIDLENFTNKNNTVRRAATKFMI
                     NTDCPSKICTIQQDYFALAMLSFAIMREHVLIFEGEDVLLNITALDKIKFCMYSEKEI
                     GNITKSQFNWLMYLLNLSEQEKLDRFEKMKYIN"
BASE COUNT          146 a           48 c           78 g          135 t
ORIGIN      
        1 gggatattta gcagtggata tttctccaac aaactttata ttggatgatt ttggtcgtgt
       61 taggttaata gacttagaaa attttacaaa caaaaacaat actgttagaa gagctgctac
      121 aaaattcatg ataaatactg attgtccctc aaaaatctgt accattcaac aggattattt
      181 tgcacttgca atgttgagtt ttgctattat gcgcgagcat gttttaatat ttgaaggaga
      241 ggacgtctta ttaaatataa ctgctttaga taaaatcaag ttttgtatgt atagcgagaa
      301 agaaattggg aacataacta aatcgcaatt taattggttg atgtatttat tgaatttaag
      361 tgaacaagag aagttagacc gattcgagaa gatgaagtat ataaacg
//
LOCUS       DAVGJL010000278          395 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.284, whole genome shotgun sequence.
ACCESSION   DAVGJL010000278 DAVGJL010000000
VERSION     DAVGJL010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.284"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(106..381)
                     /locus_tag="VVE42_002129"
     CDS             complement(106..381)
                     /locus_tag="VVE42_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEU3074240.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVEAVFAAVADYLAAGEKVQ
                     LIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFKAGKALKDAVK"
BASE COUNT          106 a           85 c           65 g          139 t
ORIGIN      
        1 ttcggaacat tcaacttttt aactctattt attactagat ttcataatta aaaagcctac
       61 tgaccaagct agaaagcttg atacaatagg ctttttaaag actgattatt taacagcgtc
      121 tttaagagct ttaccagctt tgaatgctgg tactttagaa gctgcaattg tcatttcttt
      181 accagtttgt gggttgcgac ctttacgttc tgcgcgctca cgaacttcaa agttaccaaa
      241 accgatcaac tgaacttttt caccagctgc aagatagtca gctactgctg caaatacagc
      301 ttcaactgct gctgctgagt ctttcttagt caattctgta gcttctgcta ctttagcgat
      361 caaatcttgt ttgtttgcca tgttaatgat tcctc
//
LOCUS       DAVGJL010000279          363 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.285, whole genome shotgun sequence.
ACCESSION   DAVGJL010000279 DAVGJL010000000
VERSION     DAVGJL010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.285"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
BASE COUNT          133 a           68 c           51 g          111 t
ORIGIN      
        1 tgttctacga tttttactag ataaaactgt aaattctgaa ggaaggatca cttcttcaac
       61 agaatttgga aatttcgtaa gtaatttatc attccaacac ggaatagctg gactactgtt
      121 tcctctaaat aaattgtacc ccccagaact ggattctaaa atactctcta tcatcaagaa
      181 ggcagtgaca attagaacga cacacacata tgaatatcaa tactcactgc tatttggtga
      241 tgcaggctat ctatagttac tcctacattt attttctatc agtaaaaatc aatactatct
      301 acaattagca aacgtcaccg ctaaaaaatt aatagagaat tatgatactc tagaggaaat
      361 aga
//
LOCUS       DAVGJL010000280          357 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.286, whole genome shotgun sequence.
ACCESSION   DAVGJL010000280 DAVGJL010000000
VERSION     DAVGJL010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.286"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
BASE COUNT          136 a           73 c           41 g          107 t
ORIGIN      
        1 gctatagttt tacgcccata aatacccatc aaatttaccg tctacgatat ctaaaccata
       61 tgcaatggct cctcctaatg agaagacttc agcaacagta gccgcaatat taagagcatt
      121 agtaatagtt gccatactaa ctccaccaat atatcttgtt ataacactta gttacctaga
      181 taagatatcc tttagcttgc ttccaaaatt taagtctgca cctataatat gagttcatat
      241 taatactgct aaatcctatc tatagtaatg tcaaacacct aaataaagtc atctctccaa
      301 ttcattcaag aaatgaagaa aggcaaaaaa tctttaaaat aagaaaaatg catatta
//
LOCUS       DAVGJL010000281          342 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.287, whole genome shotgun sequence.
ACCESSION   DAVGJL010000281 DAVGJL010000000
VERSION     DAVGJL010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.287"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..296
                     /locus_tag="VVE42_002130"
     CDS             <1..296
                     /locus_tag="VVE42_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU3074241.1"
                     /translation="KPEVKPQPEKPKPEVKPQPEKPKPDNSKPQADDKKPSTTNNLSK
                     DKQPSNQASTNEKATNKPKKSLPSTGSISNLALEIAGLLTLAGATILAKKRMK"
BASE COUNT          144 a           72 c           59 g           67 t
ORIGIN      
        1 caaaaccaga ggttaaaccg cagccggaaa aaccaaaacc agaggttaaa ccgcagccgg
       61 aaaaaccaaa accagataat agcaagccac aagcagatga taagaagcca tcaactacaa
      121 ataatttaag caaggacaag caaccttcta accaagcttc aacaaacgaa aaagcaacaa
      181 ataaaccgaa gaagtcattg ccatcaactg gatctatttc aaatctagca cttgaaattg
      241 caggtcttct taccttggcg ggggcaacca ttcttgctaa gaaaagaatg aaatagtgat
      301 tgattcattc aaaaatgttt gtaaaaagta ttcggcttaa aa
//
LOCUS       DAVGJL010000282          333 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.288, whole genome shotgun sequence.
ACCESSION   DAVGJL010000282 DAVGJL010000000
VERSION     DAVGJL010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.288"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(<1..>333)
                     /locus_tag="VVE42_002131"
     CDS             complement(<1..>333)
                     /locus_tag="VVE42_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3074242.1"
                     /translation="KISQFRSKVSQSPWIKYLEISSFILTIFPSILYGTVDNRVLALI
                     FLIKICVDTTIKFLFIRLFDTSTLMKRRIFFLFALDVIAYLFLGYLLVIQKAGYLFSV
                     LLLFSNFSV"
BASE COUNT          150 a           50 c           39 g           94 t
ORIGIN      
        1 aactgaaaaa ttagaaaaaa gaagtaaaac cgaaaacaaa tagcctgctt tttgaatcac
       61 taaaagatat cctaaaaata agtaagctat aacatccaat gcgaaaagaa aaaatatcct
      121 cctcttcatt aaagtacttg tgtcaaataa tctgatgaat aaaaatttta tcgtagtatc
      181 tacacaaatt tttatcaaaa agataagagc caatacacga ttatcaactg taccataaag
      241 aatagatgga aatatagtta atataaaact agaaatctct aaatatttta tccaaggaga
      301 ttgagacacc ttgcttctaa actgactaat ttt
//
LOCUS       DAVGJL010000283          333 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.289, whole genome shotgun sequence.
ACCESSION   DAVGJL010000283 DAVGJL010000000
VERSION     DAVGJL010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.289"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..>333
                     /locus_tag="VVE42_002132"
     CDS             <1..>333
                     /locus_tag="VVE42_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA helicase"
                     /protein_id="HEU3074243.1"
                     /translation="DFESDDFDWLLSLSNLNAEVFLFGDYYQKTFSTSRRGNKGKRIH
                     SSFENWLKVISDSGYEIDLSSLSKSYRCPKIVCDFIVDNLAIEILSQREEKVSSQITL
                     IDSQKKIES"
BASE COUNT          115 a           36 c           61 g          121 t
ORIGIN      
        1 ggattttgag tcagatgatt ttgattggct tttaagttta agtaatctta atgcagaagt
       61 atttttattt ggagattatt atcaaaaaac ctttagtact tcaaggcgag gtaataaagg
      121 aaaaaggatc cattcgagtt ttgagaattg gttaaaagtt atttctgatt caggatatga
      181 aattgattta tcgagtttgt caaaaagtta tcgttgtccc aaaatagttt gtgactttat
      241 tgttgataat ttagctattg aaattttatc acagcgagaa gaaaaggtat cttcccaaat
      301 tactcttatc gattcacaga aaaaaataga atc
//
LOCUS       DAVGJL010000284          328 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.290, whole genome shotgun sequence.
ACCESSION   DAVGJL010000284 DAVGJL010000000
VERSION     DAVGJL010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.290"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            30..>328
                     /locus_tag="VVE42_002133"
     CDS             30..>328
                     /locus_tag="VVE42_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU3074244.1"
                     /translation="MSYYDFVKDYMKVDECKNNEKYSSAGHTFCWKKENSTYAEGLYW
                     FYEGGSFIIDIHDFYIKEEVVQNSTYSMSDYVSIYSSYIVSANGEKFNPYQTITA"
BASE COUNT          122 a           35 c           64 g          107 t
ORIGIN      
        1 ggaatattgt ctaaaaggga tagtgtgtta tgtcatatta tgattttgta aaagattata
       61 tgaaagtaga tgaatgtaaa aacaatgaga agtattcaag tgcaggtcat acattttgtt
      121 ggaaaaaaga gaattcaact tatgcagaag gcctatattg gttttatgaa ggaggcagct
      181 tcattatcga tatacatgat ttttatatca aagaggaagt agttcaaaat agcacttata
      241 gtatgtcaga ctatgtttca atttattcaa gttatattgt cagtgcaaac ggagaaaagt
      301 ttaatccgta tcaaactatt accgcaaa
//
LOCUS       DAVGJL010000285          322 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.292, whole genome shotgun sequence.
ACCESSION   DAVGJL010000285 DAVGJL010000000
VERSION     DAVGJL010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.292"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
BASE COUNT          114 a           40 c           50 g          118 t
ORIGIN      
        1 agctagaaag gagtttactg tatcaaatct gtacagtaag attaaaatca tgaaaaagaa
       61 aacaatagca attatatagt gaaatgaaat agaaatagga taaaacaatc aggacaatca
      121 aatcaatttc tagcaatgtt ttagaagtcc agatgtacta ttctagtttc aatctattat
      181 acaatgtgtt ttgtatctca tagctcctta tatagctctt cagttatgta gtattaacag
      241 aagtttagtg ggtgagattt tcattatttt ccttattctg ttttgtttgt aggtctaagt
      301 ctttttatca ctttgaaaaa ct
//
LOCUS       DAVGJL010000286          322 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.293, whole genome shotgun sequence.
ACCESSION   DAVGJL010000286 DAVGJL010000000
VERSION     DAVGJL010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.293"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            17..>322
                     /locus_tag="VVE42_002134"
     CDS             17..>322
                     /locus_tag="VVE42_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4365 domain-containing protein"
                     /protein_id="HEU3074245.1"
                     /translation="MANNRQIETLGVSYLTTFINRHSLLQTYFGSNDKTPVWDGEIHV
                     LKTSSEKRSEIFGKVPVQIKATRQQKNKLKSFSLDISDLELYSKNGGVVLFVVWLSED
                     "
BASE COUNT          113 a           37 c           66 g          106 t
ORIGIN      
        1 ttatcgaggt tagaatttgg caaataatag acaaattgag acattaggtg tttcctatct
       61 gacaacattc ataaatagac atagtttact tcagacttat tttggtagta atgataaaac
      121 tccagtctgg gatggtgaga ttcatgttct taaaacttct agtgagaaaa gaagtgaaat
      181 atttggtaaa gtgcctgttc aaattaaagc tacaaggcaa cagaagaata aattgaaatc
      241 cttctcgttg gacataagtg atttagaatt atattcaaaa aatggtggtg tagttttatt
      301 cgtagtttgg ttaagcgagg at
//
LOCUS       DAVGJL010000287          302 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.denovo.294, whole genome shotgun sequence.
ACCESSION   DAVGJL010000287 DAVGJL010000000
VERSION     DAVGJL010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.denovo.294"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
BASE COUNT           93 a           48 c           52 g          109 t
ORIGIN      
        1 gaatgagcca ctatttattt aatatgtatg atgagggaat gactcaaaaa cttcttgtcg
       61 atataagaat atctgagata gtattttaaa acgaaaggac tataaaagct catttccttc
      121 gcacttattt tatagagatc tatcattata ctctttagaa atcaaaaagc aacacacctt
      181 gtcatcgttt tatcgtaaat ctgttaatga gggggctagt cttatctgca atctcaaatc
      241 agtgttttga gctgaatggc actcgttttc tagtttgctc tttgatttta attgggcctt
      301 ag
//
LOCUS       DAVGJL010000288         8927 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.guided.085, whole genome shotgun sequence.
ACCESSION   DAVGJL010000288 DAVGJL010000000
VERSION     DAVGJL010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8927
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.guided.085"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            32..994
                     /locus_tag="VVE42_002135"
     CDS             32..994
                     /locus_tag="VVE42_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU3074246.1"
                     /translation="MIVDPKAERGRWKETLPEISHEINIVTLTSDEKNKGLLDPYVIM
                     KNPKDSESLAIDILTFLTGISSRDSDRFPVLRKAIRAVTNSEVRGLLKVIEELRVEGT
                     QISTSIADHIESFTDYDFAHLLFSDGYVEQSISLEKQLNIIQVADLVLPDKETSFEEY
                     TTMELLSVAMLIVISTFALDFIHTDRSIFKIVDLDEAWSFLQVAQGKTLSMKLVRAGR
                     AMNAGVYFVTQNTDDLLDEKLKNNLGLKFAFRSTDLNEIKKTLAFFGVDPEDENNQKR
                     LRDLENGQCLISDLYGRVGVIQFHPVFEELLHAFDTRPPVRKEV"
     gene            997..3180
                     /locus_tag="VVE42_002136"
     CDS             997..3180
                     /locus_tag="VVE42_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEU3074247.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTALGAGSAIASATGKQGQSGSGSSARTQADHSRPD
                     GKEKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENV
                     RDLTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQE
                     QYHDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATST
                     TTADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            3177..4178
                     /locus_tag="VVE42_002137"
     CDS             3177..4178
                     /locus_tag="VVE42_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEU3074248.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            4175..5107
                     /locus_tag="VVE42_002138"
     CDS             4175..5107
                     /locus_tag="VVE42_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEU3074249.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            5426..7345
                     /gene="tet(M)"
                     /locus_tag="VVE42_002139"
     CDS             5426..7345
                     /gene="tet(M)"
                     /locus_tag="VVE42_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEU3074250.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSATHEIILSFLG
                     KVQMEVTCALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYTDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            7709..7954
                     /locus_tag="VVE42_002140"
     CDS             7709..7954
                     /locus_tag="VVE42_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3074251.1"
                     /translation="MRQIGVSYSGFVDESYTLLSLFDDVEQIEKDNRLQTAIDVVREQ
                     FGFLAIQKGTVLTEGSRNIERSKLIGGHSAGGLEGLK"
     gene            7951..8286
                     /locus_tag="VVE42_002141"
     CDS             7951..8286
                     /locus_tag="VVE42_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imsC-like protein"
                     /protein_id="HEU3074252.1"
                     /translation="MKQEKNTVQFSEIRSKGCNDIEMLERFLHGIVETATSKLRQRKL
                     KTTEISIRLVHAKSENRLPLEFTFSIKPTSSSVIIYTEVINRFKECYTGGGIQGFTIQ
                     FDKNTLASA"
     gene            8299..8667
                     /locus_tag="VVE42_002142"
     CDS             8299..8667
                     /locus_tag="VVE42_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3074253.1"
                     /translation="MIDRSYLPFQSAREYQDTKMQKWMGFFLSEHASALSDDTNKVTY
                     MSDLSLEKKLLLLSQVYAGQLRTRIQVIEKNKRVSYTGTIPSLTKDFILIKTTTGHIN
                     LKLKDIISIELVEEVLYESA"
     gene            8654..>8927
                     /locus_tag="VVE42_002143"
     CDS             8654..>8927
                     /locus_tag="VVE42_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3074254.1"
                     /translation="MNQLEFQRNHLQMDYYSESYQDFERDFYRYSNMNIPLTFLTDDI
                     LKTMATSRKNYFVLNKEKSRDNRDHFFIFEVSTVDENPLIYHYTYKK"
BASE COUNT         2939 a         1629 c         1826 g         2533 t
ORIGIN      
        1 ctattatgca gtgctttatg gggcacaagc agtgattgta gacccaaaag cagaacgtgg
       61 cagatggaaa gaaaccttgc cagagatttc ccatgaaatc aatatcgtca ctctgacttc
      121 tgatgagaaa aataaaggct tacttgaccc ttacgtgatt atgaaaaatc ccaaagattc
      181 tgaatcactg gcaatcgaca ttttgacgtt ccttacgggg atttcatcac gagattctga
      241 ccgtttccct gttctacgaa aagccattcg tgcagtaacc aatagtgaag tgcgaggtct
      301 tctgaaagtg attgaggaat tacgggttga gggaactcaa ataagcacca gcatagccga
      361 ccatatcgaa agttttacag actatgactt tgcacatctg ctctttagtg atggatatgt
      421 ggagcagtct atcagcttag aaaaacaact gaacattata caggttgcgg acttggtact
      481 tcccgacaag gaaacttcct ttgaggaata taccactatg gagcttttat ccgttgctat
      541 gctgattgtc attagtacct ttgctttaga ctttatccat acagaccgaa gcattttcaa
      601 gattgtagat ttagacgaag catggagctt tttacaggta gcacaaggaa aaacactatc
      661 tatgaagctg gttcgggctg gtcgtgctat gaacgctggg gtatatttcg tgacccaaaa
      721 tacagacgac ctcttagatg aaaaactgaa aaataacctc ggcttaaaat ttgcatttcg
      781 ttccactgac cttaacgaga ttaaaaagac cttagccttt tttggtgtag acccagagga
      841 cgaaaacaat cagaagcgat tgcgtgattt ggaaaacggg caatgcctta tcagtgattt
      901 atatggtcgt gtcggtgtga tacagttcca ccctgtattt gaagaactgc tccatgcctt
      961 tgataccaga ccacctgtgc gaaaagaggt gtaaatgtga aaccatcaat agtaaacaga
     1021 ataaaatcaa actggacgct gaaacgtcta ggtaaagtgg caatgacagt ggctttcaca
     1081 cttgtgattg ccatttttct tttagccatg ctgggaacgg tggttcaagc tgcgggcttg
     1141 gtagatgata cggtcaatgt ggcaaatgaa tacagccgat acccacttga aaactatcaa
     1201 ctggattttt atgtggataa tagctggggc tggcttccgt ggaactggtc ggacgggatt
     1261 ggaaaacagg tcatgtatgg actatatgcc attaccaatt ttatttggac aatcagtttg
     1321 tatgtttcca atgcgacagg ttacttagta caggaagcct attccttaga cttcatttcc
     1381 gctacagcag attccattgg taagaatatg cagaccttag ctggtgtgag tgcaaacgga
     1441 ttttcaacag agggtttcta tgttggattc ctcttactct tgattttggt tcttggggtt
     1501 tatgttgcct atacgggact gataaagaga gaaaccacaa aggcaattca tgccattatg
     1561 aattttgtgc tggtgtttat cctatcggct tcctttattg cctacgctcc cgactacatt
     1621 aaaaaaatca atgacttttc atcagacatc agtaatgcca gtttatcact tggcacgaag
     1681 attgtcatgc cccattccga tagtcaaggc aaggacagcg tggacttaat cagagatagc
     1741 ctgttttcca tacaggttca gcaaccgtgg ctactgcttc aatacaacag ttcagacatt
     1801 gaaagtatcg gtattgaccg tgtggaaagc ctgctctcca ccagcccaga ttccaacaat
     1861 ggcgaagaca gagaaaaaat tgttgcggaa gaaattgaag acagaagcaa taccaatcta
     1921 accattacaa agaccattaa ccgtttaggt acagtcttct tcctatttgt cttcaatatt
     1981 gggatttcca tatttgtatt cctattaaca ggaatcatga ttttctcgca ggtacttttt
     2041 atcatctatg ctatgtttct gcctgtgagc tttattttaa gcatgattcc atcatttgat
     2101 ggtatgtcaa aacgagccat aacaaagctc tttaatacca ttttgacacg agctggaatc
     2161 acattgatta ttacgacagc atttagtatt tcaaccatgc tctatacctt atcggctggt
     2221 tatccgttct ttttgattgc ttttctacag attgtgacct ttgcaggaat ctacttcaag
     2281 ctgggcgatt taatgagtat gttttctcta cagagtaacg attctcaaag tgtgggaagt
     2341 cgtgtgatga gaaaacctcg tatgcttatg cacgctcata tgcaccgcct acagcgtaaa
     2401 cttggacgct ccatgacagc tttaggtgct ggctctgcca ttgccagtgc tacaggaaaa
     2461 caaggacagt cgggttcggg gagttctgca aggacacaag cagatcactc ccgaccagac
     2521 ggaaaggaaa aatcaacact tggaaaacgt atcggtcaaa ccatcggtac agtagctgat
     2581 accaaagaca gaatggtaga cactgctagt ggtttgaaag aacaggttaa agatttgccg
     2641 accaatgcaa gatatgcagt atatcaagga aaatccaaag taaaagagaa tgtccgtgat
     2701 ttaaccagta gtatttctca aaccaaagcg gacagagcca gtggacgcaa ggaacagcag
     2761 gaacaaaggc gaaaaaccat tgcgaagcgt cgctctgaaa tggaacaggt caaacagaaa
     2821 aaacagcctg cttcttctgt tcatgaaaga ccgactacaa gacaagaaca atatcatgat
     2881 gaacagacct caaaacagtc taatattcag acttcatata aggaatctca acaagccaaa
     2941 caagagcgtc cagcagttaa gtccgatttt tcaagtccaa aagtggaacg ccaaggcaat
     3001 accgttcaag aaaaaaccgt tcaaaagcca gcaacttcaa ccactacagc agatagaact
     3061 tcacaacgtc caatcacaaa agaacgtccg tctactgttc aaagagtacc actacaaaat
     3121 acaagaagta gaccaccaat caaaaccgcc accattaaga aagtcggtaa gaaaccatga
     3181 agttgaaaac tttagtgatt ggtggttctg gattattctt gatggtcttc tcactgcttc
     3241 tgtttgttgc cattttattt tcagatgaac aggacagcgg aatttccaat attcattatg
     3301 gaggtgtgaa tgtttccgca gaagtgctgg ctcataagcc tatggtagaa aaatatgcca
     3361 aagaatatgg cgttgaagaa tatgtcaaca tacttcttgc gattatacag gtggaatcgg
     3421 gcggtactgc ggaagatgtt atgcagtcct cggaatccct cggtcttcca cctaattcat
     3481 tgagtacaga agaatccatt aagcaaggtg tgaagtattt cagtgaatta ttagccagta
     3541 gcgaaaggct cagtgtagat ttagaatcgg ttatccagtc ctacaattat ggtggtggtt
     3601 tcttagggta tgtggctaat cgtggaaata aatatacctt tgaactggct caaagtttct
     3661 caaaagagta ttcaggtggc gaaaaagtgt cttaccccaa tcccatagcc atacctatca
     3721 atgggggctg gcgatacaac tatggcaata tgttttatgt gcaactggta acgcagtatc
     3781 ttgtcacaac agagtttgat gatgatacgg tacaagccat catggacgaa gcactgaaat
     3841 atgagggctg gcgatacgtt tacggtggag cttccccgac tacttctttt gattgtagcg
     3901 gactgacaca atggacgtat ggaaaagctg gaattaactt accacgaacc gcacaacagc
     3961 aatatgatgt gacccagcat atcccactat cggaagcaca agctggcgat ttggttttct
     4021 ttcattctac ctataacgct ggctcttata ttactcatgt tgggatatac cttggcaata
     4081 accgtatgtt tcatgcaggc gacccaatcg gttatgccga cttaacaagc ccctactggc
     4141 aacagcattt agtgggagca ggacgaatca aacaatgaga aaggaagatt taatgatgaa
     4201 atttagaaaa aatcagaata aagaaaaaca gataccaaag gaaaagaaac ctcgtgtcta
     4261 taaggtcaat cctcataaaa aggttgtgat tgccttgtgg gtacttttag ggcttagttt
     4321 cagctttgcg atattcaagc actttacagc tatagatact catactattc acgaaacaac
     4381 tatcatagaa aaggaatacg ttgatactca tcatgtagaa aattttgtag agaactttgc
     4441 gaaagtctac tattcatggg agcaatccga taagtccatt gataatcgaa tggaaagtct
     4501 aaaaggctat ctgacagatg aacttcaagc tctcaatgtt gatacagtac gcaaagatat
     4561 tcctgtatcg tcttctgtaa gaggatttca gatatggacg gtagagccaa ctggcgacaa
     4621 tgagtttaat gtaacctaca gtgtagacca gctcattaca gagggagaaa atacaaagac
     4681 cgtccactct gcttatatag tgagtgtcta tgtagatggt tctggaaata tggtactggt
     4741 taagaatccg accattacca acatacctaa gaaatcaagt tataaaccaa aagccattga
     4801 aagtgagggg acggttgatt ccattacaac caatgaaatc aatgagtttt taacgacgtt
     4861 cttcaagctc tatcctacag cgacagccag tgaactttcc tactatgtga atgacgggat
     4921 attaaaacca atcggaaaag agtacatctt tcaagaactg gtaaatccta ttcacaatcg
     4981 taaggataat caagtcacgg tatcgctgac agtggagtat atcgaccagc agaccaaagc
     5041 aacgcaggta tctcaatttg atttggtact tgaaaagaac gggagtaatt ggaagattat
     5101 agaataacaa atattggtac attattacag ctattttgta atcacgtact ctctttgata
     5161 aaaaattgga gattccttga caaatatgct cttatgtgct attatttaag tatctattta
     5221 aaaggagtta ataaatatgc ggcaaggtat tcttaaataa actgtcaatt tgatagtggg
     5281 aacaaataat tggatgtcct tttttaggag ggcttagttt tttgtaccca gtttaagaat
     5341 acctttatca tgtgattcta aagtatccgg agaatatctg tatgcttttt atgccctttt
     5401 gggtttttga atggaggaaa atcacatgaa aattattaat attggagttt tagctcatgt
     5461 tgatgcagga aaaactacct taacagaaag cttattatat aacagtggag cgattacaga
     5521 attaggaagc gtggacaaag gtacaacgag gacggataat acgcttttag aacgtcagag
     5581 aggaattaca attcagacag gaataacctc ttttcagtgg gaaaatacga aggtgaacat
     5641 catagacacg ccaggacata tggatttctt agcagaagta tatcgttcat tatcagtttt
     5701 agatggggca attctactga tttctgcaaa agatggcgta caagcacaaa ctcgtatatt
     5761 atttcatgca cttaggaaaa tggggattcc cacaatcttt tttatcaata agattgacca
     5821 aaatggaatt gatttatcaa cggtttatca ggatattaaa gagaaacttt ctgccgaaat
     5881 tgtaatcaaa cagaaggtag aactgtatcc taatatgtgt gtgacgaact ttaccgaatc
     5941 tgaacaatgg gatacggtaa tagagggaaa cgatgacctt ttagagaaat atatgtccgg
     6001 taaatcatta gaagcattgg aactcgaaca agaggaaagc ataagatttc agaattgttc
     6061 tctgttccct ctttatcacg gaagtgcaaa aaacaatata gggattgata accttataga
     6121 agtgattacg aataaatttt attcatcaac acatcgaggt ccgtctgaac tttgcggaaa
     6181 tgttttcaaa attgaatata caaaaaaaag acaacgtctt gcatatatac gcctttatag
     6241 tggagtacta catttacgag attcggttag agtatcagaa aaagaaaaaa taaaagttac
     6301 agaaatgtat acttcaataa atggtgaatt atgtaagatt gatagagctt attctggaga
     6361 aattgttatt ttgcaaaatg agtttttgaa gttaaatagt gttcttggag atacaaaact
     6421 attgccacag agaaaaaaga ttgaaaatcc gcaccctcta ctacaaacaa ctgttgaacc
     6481 gagtaaacct gaacagagag aaatgttgct tgatgcactt ttagaaatct ccgacagtga
     6541 cccgcttctg cgatattatg tggattctgc gacacatgaa atcatacttt ctttcttagg
     6601 gaaagtacaa atggaagtga cttgtgctct gctgcaagaa aagtatcatg tggagataga
     6661 aataaaagag cctacagtca tttatatgga aagaccgtta aaaaaagcag agtataccat
     6721 tcacatcgaa gttccaccga atcctttctg ggcttccatt ggtttatctg tatcaccgct
     6781 tccgttggga agtggaatgc agtatgagag ctcggtttct cttggatact taaatcaatc
     6841 atttcaaaat gcagttatgg aagggatacg ctatggctgt gaacaaggat tgtatggttg
     6901 gaatgtgacg gactgtaaaa tctgttttaa gtatggctta tactatagcc ctgttagtac
     6961 cccagcagat tttcggatgc ttgctcctat tgtattggaa caagtcttaa aaaaagctgg
     7021 aacagaattg ttagagccat atcttagttt taaaatttat gcgccacagg aatatctttc
     7081 acgagcatac accgatgctc ctaaatattg tgcgaacatc gtagacactc aattgaaaaa
     7141 taatgaggtc attcttagtg gagaaatccc tgctcggtgt attcaagaat atcgtagtga
     7201 tttaactttc tttacaaatg gacgtagtgt ttgtttaaca gagttaaaag ggtaccatgt
     7261 tactaccggt gaacctgttt gccagccccg tcgtccaaat agtcggatag ataaagtacg
     7321 atatatgttc aataaaataa cttagtgtat tttatgttgt tatataaata tggtttcttg
     7381 ttaaataaga tgaaatattt tttaataaag atttgaatta aagtgtaaag gaggagatag
     7441 ttattataaa ctacaagtgg atattgtgtg ctgagagctt tcttctatac taatagacga
     7501 aagggtgtga aaatgatttt taaatgatac tgtggaacgg aacagtagcc ctagtattga
     7561 ctactgtcgt ttctattcat attggctatt ctaggactga gatgaaaaaa tctataaatg
     7621 ctcagaataa aattgaaccc gcaaatctcc ccaaaacaat ggtgagtcat gtacttgtat
     7681 tattccgaaa aaatacacct ctggtgcagt gagacaaatt ggtgtatctt atagtggctt
     7741 cgtagatgaa agctatactc tactatcact ctttgatgat gtagaacaaa ttgaaaaaga
     7801 taatagactt cagacagcta ttgatgttgt cagagaacag tttggttttt tagccataca
     7861 aaaaggaacc gtcctaactg aaggttccag aaatattgaa cgcagtaaac ttatcggtgg
     7921 tcattccgcg ggtggattgg agggattaaa atgaaacaag aaaaaaatac agtacaattt
     7981 tcagaaatcc gtagcaaagg atgtaatgat attgaaatgc ttgaaagatt tttacatgga
     8041 atcgttgaaa cagcaacttc aaaacttcgt cagagaaaac tcaaaacaac tgaaatatcg
     8101 atacgactag tacatgctaa atctgaaaac cgattaccat tggaatttac atttagcatt
     8161 aagccaacaa gctcatctgt gataatctat actgaggtaa tcaatcgctt taaagaatgt
     8221 tacacaggtg ggggaattca aggttttacg attcaatttg ataaaaatac ccttgcctct
     8281 gcatagaaag gatttgatat gattgaccgt tcatatttac catttcaatc agcaagagag
     8341 taccaggata caaagatgca aaaatggatg ggctttttcc tatctgaaca tgcatcagca
     8401 ctctctgatg atacaaacaa agtaacgtac atgtctgact tatcactaga gaagaaatta
     8461 ttactcctca gtcaagtata cgccgggcag ctacgcacac gcattcaagt gattgaaaaa
     8521 aacaagcgtg tttcctacac tggaacaata ccaagtctga ccaaagattt cattttgata
     8581 aaaactacaa caggtcacat caatttgaaa ttaaaagaca ttattagtat tgaacttgtc
     8641 gaggaggtgc tctatgaatc agcttgagtt tcagcgtaat cacctacaaa tggactatta
     8701 tagcgagagc taccaagatt ttgaacgtga cttctaccgc tactctaaca tgaatattcc
     8761 attgaccttc ctaactgatg atatcctaaa aacaatggcg acttcacgta agaattactt
     8821 tgtcctcaat aaggaaaagt ccagagataa ccgcgatcac ttcttcatat ttgaagtaag
     8881 taccgtagat gagaatccgc taatctatca ttatacatat aagaaaa
//
LOCUS       DAVGJL010000289         1838 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.guided.184, whole genome shotgun sequence.
ACCESSION   DAVGJL010000289 DAVGJL010000000
VERSION     DAVGJL010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.guided.184"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            42..212
                     /locus_tag="VVE42_002144"
     CDS             42..212
                     /locus_tag="VVE42_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HEU3074255.1"
                     /translation="MKYFEVELENPDEFLKLQTEDFVKANRLLLRKIIQSVTVYEENF
                     VISFKSGIELEV"
     gene            598..1815
                     /gene="mef(A)"
                     /locus_tag="VVE42_002145"
     CDS             598..1815
                     /gene="mef(A)"
                     /locus_tag="VVE42_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(A)"
                     /protein_id="HEU3074256.1"
                     /translation="MEKYNNWKRKFYAIWAGQAVSLITSAILQMAIIFYLTEKTGSAM
                     VLSMASLVGFLPYAILGPAIGVLVDRHDRKKIMIGADLIIAAAGAVLAIVAFCMELPV
                     WMIMIVLFIRSIGTAFHTPALNAVTPLLVPEEQLTKCAGYSQSLQSISYIVSPAVAAL
                     LYSVWDLNAIIAIDVLGAVIASITVAIVRIPKLGNQVQSLEPNFIREMKEGVVVLRQN
                     KGLFALLLLGTLYTFVYMPINALFPLISMEHFNGTPVHISITEISFAFGMLAGGLLLG
                     RLGGFEKHVLLITSSFFIMGTSLAVSGILPPNGFVIFVVCCAIMGLSVPFYSGVQTAL
                     FQEKIKPEYLGRVFSLIGSIMSLAMPIGLILSGFFADKIGVNHWFLLSGILIIGIAIV
                     CQMITEVRKLDLK"
BASE COUNT          567 a          279 c          375 g          617 t
ORIGIN      
        1 gcaaactcaa tttatcaata aatgaaatga gggaatgtaa aatgaaatat tttgaggttg
       61 agttagaaaa tcctgatgaa tttttaaaac tacaaacaga agattttgtg aaagctaatc
      121 gcttgctact aaggaagata atccagagcg ttacagtcta tgaagaaaac ttcgtcatat
      181 cctttaaatc tggcatcgaa ttggaagtat gagtctcatt ccataacttt tatattgaac
      241 atatcatctt gttgtgttat actataaatt gatataaaca aagatgtagg aggaaccgaa
      301 actatgacag cctcaatgcg tttaagataa gctggcaata aaaaaagcag aatctatacc
      361 cgatgatagg cttttttgtt gtgcttattt atacgatatt gagcattcat tagttacggt
      421 gaggatattg gttatttaac tataccttta tttaactatg tctttaatat gaatgtttcc
      481 aaattgtatg tatgcagacc aaaagccaca ttgtggggtt tggcctgcat tttttattgc
      541 ctagaatgct attcaaaata gaaattcaag caaaataata tgcaggagat aatataaatg
      601 gaaaaataca acaattggaa acgaaaattt tatgcaatat gggcagggca agcagtatca
      661 ttaatcacta gtgccatcct gcaaatggcg attatttttt accttacaga aaaaacagga
      721 tctgcgatgg tcttgtctat ggcttcatta gtaggttttt taccctatgc gattttggga
      781 cctgccattg gtgtgctagt ggatcgtcat gataggaaga agataatgat tggtgccgat
      841 ttaattatcg cagcagctgg tgcagtgctt gctattgttg cattctgtat ggagctacct
      901 gtctggatga ttatgatagt attgtttatc cgtagcattg gaacagcttt tcatacccca
      961 gcactcaatg cggttacacc acttttagta ccagaagaac agctaacgaa atgcgcaggc
     1021 tatagtcagt ctttgcagtc tataagctat attgttagtc cggcagttgc agcactctta
     1081 tactccgttt gggatttaaa tgctattatt gccatcgacg tattgggtgc tgtgattgca
     1141 tctattacgg tagcaattgt acgtatacct aagctgggta atcaagtgca aagtttagaa
     1201 ccaaatttca taagggagat gaaagaagga gttgtggttc tgagacaaaa caaaggattg
     1261 tttgccttat tactcttagg aacactatat acttttgttt atatgccaat caatgcacta
     1321 tttcctttaa taagcatgga acactttaat ggaacgcctg tgcatatttc tattacggaa
     1381 atttcctttg catttgggat gctagcagga ggcttattat taggaagatt agggggcttc
     1441 gaaaagcatg tattactaat aacaagttca ttttttataa tggggaccag tttagccgtt
     1501 tcgggaatac ttcctccaaa tggatttgta atattcgtag tttgctgtgc aataatgggg
     1561 ctttcggtgc cattttatag cggtgtgcaa acagctcttt ttcaggagaa aattaagcct
     1621 gaatatttag gacgtgtatt ttctttgatc ggaagtatca tgtcacttgc tatgccaatt
     1681 gggttaattc tttctggatt ctttgctgat aaaatcggtg taaatcattg gtttttacta
     1741 tcaggtattt taattattgg cattgctata gtttgccaaa tgataactga ggttagaaaa
     1801 ttagatttaa aataaacaat attggaggaa tatttatg
//
LOCUS       DAVGJL010000290         1623 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.guided.193, whole genome shotgun sequence.
ACCESSION   DAVGJL010000290 DAVGJL010000000
VERSION     DAVGJL010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.guided.193"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            91..1554
                     /gene="msr(D)"
                     /locus_tag="VVE42_002146"
     CDS             91..1554
                     /gene="msr(D)"
                     /locus_tag="VVE42_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(D)"
                     /protein_id="HEU3074257.1"
                     /translation="MELILKAKDIRVEFKGRDVLDINELEVYDYDRIGLVGANGAGKS
                     TLLRVLLGELTPPGCKMNRLGELAYIPQLDEVTLQEEKDFALVGKLGVEQLNIQTMSG
                     GEETRLKIAQALSAQVHGILADEPTSHLDREGIDFLIGQLKYFTGALLVISHDRYFLD
                     EIVDKIWELKDGKITEYWGNYSDYLRQKEEERKSQAAEYEQFIAERARLERAAEEKRK
                     QARKIEQKAKGSSKKKSTEDGGRLAHQKSIGSKEKKMYNAAKTLEHRIAALGKVEAPE
                     GIRRIRFRQSKALELHNPYPIVGAEINKVFGDKALFENASFQIPLGAKVALTGGNGIG
                     KTTLIQMILNHEEGISISPKAKIGYFAQNGYKYNSNQNVMEFMQKDCDYNISEIRSVL
                     ASMGFKQNDIGKSLSVLSGGEIIKLLLAKMLMGRYNILIMDEPSNFLDIPSLEALEIL
                     MKEYTGTIVFITHDKRLLENVADVVYEIRDKKINLKH"
BASE COUNT          575 a          245 c          371 g          432 t
ORIGIN      
        1 attttcatgt aactcttcct gctaaaatcg cagggttttc cctgcataca agcaaatgaa
       61 agcatgcgat tatagacagg aggaaatgtt atggaattaa tattaaaagc aaaagacatt
      121 cgtgtggaat tcaaaggacg cgatgtttta gatataaatg aattagaagt atatgattat
      181 gaccgtattg gtttagtagg agcaaatggt gctggaaaaa gcactttact cagggtactt
      241 ttaggagaat taactccccc aggatgtaaa atgaatcgtc tgggtgaact tgcctatatt
      301 ccccagttgg acgaagtaac tctgcaggag gaaaaagatt ttgcacttgt aggcaagcta
      361 ggtgttgagc aattaaatat acagactatg agcggtggtg aagaaacaag gcttaaaata
      421 gcacaggcct tatcggcaca ggttcatggt attttagcgg atgaacctac gagccattta
      481 gaccgtgaag gaattgattt tctaatagga cagctaaaat attttacagg tgcactgtta
      541 gttattagcc atgaccgcta ttttcttgat gaaatagtag ataaaatatg ggaactgaaa
      601 gatggcaaaa tcactgagta ttggggaaac tattctgatt atcttcgtca gaaagaggaa
      661 gaacgtaaga gccaagctgc agaatacgaa caatttattg cggaacgtgc ccgattggaa
      721 agggctgcgg aggaaaagcg aaaacaggct cgtaaaatag aacagaaggc aaaaggttct
      781 tcaaagaaaa aaagtactga agacggaggg cgtttagctc atcaaaaatc aataggaagt
      841 aaggaaaaaa agatgtataa tgctgctaaa accctagagc acaggattgc ggccttagga
      901 aaagtagaag ctccggaagg cattcgcaga attcgtttca ggcaaagtaa agcattggag
      961 ctccataatc cataccctat agtcggtgca gaaattaata aagtatttgg ggataaggct
     1021 ctgtttgaaa atgcatcttt tcaaattccg ttaggagcaa aagtggcgtt aactggtggt
     1081 aatggaatcg gaaaaacaac tttaatccaa atgatcttaa accatgaaga aggaatttct
     1141 atttcgccta aggcaaaaat aggttacttt gcacagaatg gttacaagta caacagtaat
     1201 cagaatgtta tggagtttat gcagaaggat tgtgactaca atatatcaga aattcgttca
     1261 gtgctagcat ctatggggtt caaacagaac gatattggaa aaagtttatc tgttttaagc
     1321 ggtggagaaa ttataaaatt gttgcttgct aaaatgctca tgggtagata taacatccta
     1381 ataatggatg aacccagtaa cttccttgac ataccaagtt tagaggcttt ggaaatacta
     1441 atgaaggagt acaccggaac tatcgtgttt atcacccacg ataaacgatt actcgaaaat
     1501 gtagcagatg tagtttatga aattagagat aagaaaataa atctgaaaca ttaaatttaa
     1561 ggtagtcgct ggtcagtata gtctgttctg gttggcgact ccattgttaa agagtataaa
     1621 gac
//
LOCUS       DAVGJL010000291         1068 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3471
            SAMD00182593-rid19694623.guided.218, whole genome shotgun sequence.
ACCESSION   DAVGJL010000291 DAVGJL010000000
VERSION     DAVGJL010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182593
            Sequence Read Archive: DRR189356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925884.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925884.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 18:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 53 of 124
            Pseudo Genes (internal stop)      :: 42 of 124
            Pseudo Genes (multiple problems)  :: 26 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182593-rid19694623.guided.218"
                     /strain="SP3471"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            131..868
                     /gene="erm(B)"
                     /locus_tag="VVE42_002147"
     CDS             131..868
                     /gene="erm(B)"
                     /locus_tag="VVE42_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEU3074258.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          398 a          176 c          169 g          325 t
ORIGIN      
        1 aaataaatac gttagattaa ttcctaccag tgactaatct tatgactttt taaacagata
       61 actaaaatta caaacaaatc gtttaacttc tgtatttatt tatagatgta atcacttcag
      121 gagtgattac atgaacaaaa atataaaata ttctcaaaac tttttaacga gtgaaaaagt
      181 actcaaccaa ataataaaac aattgaattt aaaagaaacc gataccgttt acgaaattgg
      241 aacaggtaaa gggcatttaa cgacgaaact ggctaaaata agtaaacagg taacgtctat
      301 tgaattagac agtcatctat tcaacttatc gtcagaaaaa ttaaaactga acattcgtgt
      361 cactttaatt caccaagata ttctacagtt tcaattccct aacaaacaga ggtataaaat
      421 tgttgggaat attccttacc atttaagcac acaaattatt aaaaaagtgg tttttgaaag
      481 ccatgcgtct gacatctatc tgattgttga agaaggattc tacaagcgta ccttggatat
      541 tcaccgaaca ctagggttgc tcttgcacac tcaagtctcg attcagcaat tgcttaagct
      601 gccagcggaa tgctttcatc ctaaaccaaa agtaaacagt gtcttaataa aacttacccg
      661 ccataccaca gatgttccag ataaatattg gaagctatat acgtactttg tttcaaaatg
      721 ggtcaatcga gaatatcgtc aactgtttac taaaaatcag tttcatcaag caatgaaaca
      781 cgccaaagta aacaatttaa gtaccgttac ttatgagcaa gtattgtcta tttttaatag
      841 ttatctatta tttaacggga ggaaataatt ctatgagtcg cttttgtaaa tttggaaagt
      901 tacacgttac taaagggaat gtagataaat tattaggtat actactgaca gcttccaagg
      961 agctaaagag gtccctagcg cctacgggga atttgtatcg ataaggaata gatttaaaaa
     1021 tttcgctgtt attttgtaca ataaggataa atttgaatgg taccataa
//
